LOCUS       DAXLPI010000001        71098 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXLPI010000001 DAXLPI010000000
VERSION     DAXLPI010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.001"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            178..408
                     /locus_tag="ACF3T5_000001"
     CDS             178..408
                     /locus_tag="ACF3T5_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFD3630648.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            505..1806
                     /gene="murA"
                     /locus_tag="ACF3T5_000002"
     CDS             505..1806
                     /gene="murA"
                     /locus_tag="ACF3T5_000002"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFD3630649.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1819..1995
                     /locus_tag="ACF3T5_000003"
     CDS             1819..1995
                     /locus_tag="ACF3T5_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFD3630650.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2130..2426
                     /gene="yidD"
                     /locus_tag="ACF3T5_000004"
     CDS             2130..2426
                     /gene="yidD"
                     /locus_tag="ACF3T5_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFD3630651.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2611..3078
                     /locus_tag="ACF3T5_000005"
     CDS             2611..3078
                     /locus_tag="ACF3T5_000005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3630652.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3163..4047
                     /locus_tag="ACF3T5_000006"
     CDS             3163..4047
                     /locus_tag="ACF3T5_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFD3630653.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4161..4397
                     /locus_tag="ACF3T5_000007"
     CDS             4161..4397
                     /locus_tag="ACF3T5_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFD3630654.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4485..4565
                     /locus_tag="ACF3T5_000008"
     tRNA            4485..4565
                     /locus_tag="ACF3T5_000008"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4519..4521,aa:Tyr,seq:gta)
     gene            4572..4643
                     /locus_tag="ACF3T5_000009"
     tRNA            4572..4643
                     /locus_tag="ACF3T5_000009"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4604..4606,aa:Gln,seq:ttg)
     gene            4768..4938
                     /locus_tag="ACF3T5_000010"
     CDS             4768..4938
                     /locus_tag="ACF3T5_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630655.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            5158..5865
                     /locus_tag="ACF3T5_000011"
     CDS             5158..5865
                     /locus_tag="ACF3T5_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3630656.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5924..6568)
                     /locus_tag="ACF3T5_000012"
     CDS             complement(5924..6568)
                     /locus_tag="ACF3T5_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFD3630657.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6694..6981
                     /locus_tag="ACF3T5_000013"
     CDS             6694..6981
                     /locus_tag="ACF3T5_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630658.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7089..7409
                     /locus_tag="ACF3T5_000014"
     CDS             7089..7409
                     /locus_tag="ACF3T5_000014"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3630659.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7490..8804
                     /locus_tag="ACF3T5_000015"
                     /pseudo
     CDS             7490..8804
                     /locus_tag="ACF3T5_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            8801..9520
                     /locus_tag="ACF3T5_000016"
     CDS             8801..9520
                     /locus_tag="ACF3T5_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFD3630660.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            9648..10199
                     /gene="raiA"
                     /locus_tag="ACF3T5_000017"
     CDS             9648..10199
                     /gene="raiA"
                     /locus_tag="ACF3T5_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFD3630661.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10421..12955
                     /gene="secA"
                     /locus_tag="ACF3T5_000018"
     CDS             10421..12955
                     /gene="secA"
                     /locus_tag="ACF3T5_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFD3630662.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13081..14182
                     /gene="prfB"
                     /locus_tag="ACF3T5_000019"
     CDS             join(13081..13152,13154..14182)
                     /gene="prfB"
                     /locus_tag="ACF3T5_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFD3630663.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14287..14973
                     /gene="ftsE"
                     /locus_tag="ACF3T5_000020"
     CDS             14287..14973
                     /gene="ftsE"
                     /locus_tag="ACF3T5_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFD3630664.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14966..15850
                     /gene="ftsX"
                     /locus_tag="ACF3T5_000021"
     CDS             14966..15850
                     /gene="ftsX"
                     /locus_tag="ACF3T5_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFD3630665.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            16002..17450
                     /gene="cls"
                     /locus_tag="ACF3T5_000022"
     CDS             16002..17450
                     /gene="cls"
                     /locus_tag="ACF3T5_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFD3630666.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17787..18644
                     /locus_tag="ACF3T5_000023"
     CDS             17787..18644
                     /locus_tag="ACF3T5_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFD3630667.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18759..19679
                     /gene="pstC"
                     /locus_tag="ACF3T5_000024"
     CDS             18759..19679
                     /gene="pstC"
                     /locus_tag="ACF3T5_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFD3630668.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19679..20563
                     /gene="pstA"
                     /locus_tag="ACF3T5_000025"
     CDS             19679..20563
                     /gene="pstA"
                     /locus_tag="ACF3T5_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFD3630669.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20578..21384
                     /gene="pstB"
                     /locus_tag="ACF3T5_000026"
     CDS             20578..21384
                     /gene="pstB"
                     /locus_tag="ACF3T5_000026"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFD3630670.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21400..22158
                     /gene="pstB"
                     /locus_tag="ACF3T5_000027"
     CDS             21400..22158
                     /gene="pstB"
                     /locus_tag="ACF3T5_000027"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFD3630671.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22171..22848
                     /gene="phoU"
                     /locus_tag="ACF3T5_000028"
     CDS             22171..22848
                     /gene="phoU"
                     /locus_tag="ACF3T5_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFD3630672.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23425..24117
                     /locus_tag="ACF3T5_000029"
     CDS             23425..24117
                     /locus_tag="ACF3T5_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFD3630673.1"
                     /translation="MLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISIL
                     FVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVI
                     GSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPIS
                     VLFFLTVRRLKR"
     gene            24114..24881
                     /locus_tag="ACF3T5_000030"
     CDS             24114..24881
                     /locus_tag="ACF3T5_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFD3630674.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24981..26549
                     /gene="liaX"
                     /locus_tag="ACF3T5_000031"
     CDS             24981..26549
                     /gene="liaX"
                     /locus_tag="ACF3T5_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFD3630675.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26846..27166
                     /locus_tag="ACF3T5_000032"
     CDS             26846..27166
                     /locus_tag="ACF3T5_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFD3630676.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            27166..27516
                     /locus_tag="ACF3T5_000033"
     CDS             27166..27516
                     /locus_tag="ACF3T5_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFD3630677.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27683..28621
                     /gene="hprK"
                     /locus_tag="ACF3T5_000034"
     CDS             27683..28621
                     /gene="hprK"
                     /locus_tag="ACF3T5_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFD3630678.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28636..29466
                     /gene="lgt"
                     /locus_tag="ACF3T5_000035"
     CDS             28636..29466
                     /gene="lgt"
                     /locus_tag="ACF3T5_000035"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFD3630679.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29510..30535
                     /locus_tag="ACF3T5_000036"
     CDS             29510..30535
                     /locus_tag="ACF3T5_000036"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFD3630680.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30550..31488
                     /gene="galU"
                     /locus_tag="ACF3T5_000037"
     CDS             30550..31488
                     /gene="galU"
                     /locus_tag="ACF3T5_000037"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFD3630681.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31613..32053
                     /locus_tag="ACF3T5_000038"
     CDS             31613..32053
                     /locus_tag="ACF3T5_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFD3630682.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32056..32658
                     /locus_tag="ACF3T5_000039"
     CDS             32056..32658
                     /locus_tag="ACF3T5_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFD3630683.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32717..33823)
                     /locus_tag="ACF3T5_000040"
     CDS             complement(32717..33823)
                     /locus_tag="ACF3T5_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HFD3630684.1"
                     /translation="MNNVKINELRQWMAQHQVELSYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34033..35034
                     /gene="ccpA"
                     /locus_tag="ACF3T5_000041"
     CDS             34033..35034
                     /gene="ccpA"
                     /locus_tag="ACF3T5_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFD3630685.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35371..37716)
                     /locus_tag="ACF3T5_000042"
     CDS             complement(35371..37716)
                     /locus_tag="ACF3T5_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFD3630686.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37995..38897
                     /locus_tag="ACF3T5_000043"
     CDS             37995..38897
                     /locus_tag="ACF3T5_000043"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFD3630687.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39110..40885
                     /locus_tag="ACF3T5_000044"
     CDS             39110..40885
                     /locus_tag="ACF3T5_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFD3630688.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40951..42537)
                     /locus_tag="ACF3T5_000045"
     CDS             complement(40951..42537)
                     /locus_tag="ACF3T5_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFD3630689.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42788..43012
                     /locus_tag="ACF3T5_000046"
     CDS             42788..43012
                     /locus_tag="ACF3T5_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFD3630690.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43154..44905
                     /locus_tag="ACF3T5_000047"
     CDS             43154..44905
                     /locus_tag="ACF3T5_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFD3630691.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLMLDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44905..46689
                     /locus_tag="ACF3T5_000048"
     CDS             44905..46689
                     /locus_tag="ACF3T5_000048"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3630692.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIITRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTSDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMMKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPIEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVEHGNHETLLAQNGLYADMYKLQSSGI"
     gene            47015..47866
                     /gene="licT"
                     /locus_tag="ACF3T5_000049"
     CDS             47015..47866
                     /gene="licT"
                     /locus_tag="ACF3T5_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFD3630693.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEDLQVHLNDSVYIALTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDKAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRCYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47961..49847
                     /locus_tag="ACF3T5_000050"
     CDS             47961..49847
                     /locus_tag="ACF3T5_000050"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFD3630694.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVRVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTVARKFKVNEFVGMLLGAALMNPNLSLEVLSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVNYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAMPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKDEAGETDDIQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(49887..50789)
                     /locus_tag="ACF3T5_000051"
     CDS             complement(49887..50789)
                     /locus_tag="ACF3T5_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFD3630695.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51020..52423
                     /locus_tag="ACF3T5_000052"
     CDS             51020..52423
                     /locus_tag="ACF3T5_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFD3630696.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLDIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETITSIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETIGRLKEEGIEVQ
                     DELARELSSNTHIMEEV"
     gene            52423..53856
                     /locus_tag="ACF3T5_000053"
     CDS             52423..53856
                     /locus_tag="ACF3T5_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFD3630697.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWDSRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            54001..54927
                     /gene="trxB"
                     /locus_tag="ACF3T5_000054"
     CDS             54001..54927
                     /gene="trxB"
                     /locus_tag="ACF3T5_000054"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFD3630698.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55142..56869
                     /locus_tag="ACF3T5_000055"
     CDS             55142..56869
                     /locus_tag="ACF3T5_000055"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFD3630699.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EENHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAKKVASYE
                     AIIKKMTEE"
     gene            56884..57234
                     /locus_tag="ACF3T5_000056"
     CDS             56884..57234
                     /locus_tag="ACF3T5_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFD3630700.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(57326..58186)
                     /locus_tag="ACF3T5_000057"
     CDS             complement(57326..58186)
                     /locus_tag="ACF3T5_000057"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFD3630701.1"
                     /translation="MTSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58278..58706
                     /locus_tag="ACF3T5_000058"
     CDS             58278..58706
                     /locus_tag="ACF3T5_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFD3630702.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58868..59044
                     /gene="rpsU"
                     /locus_tag="ACF3T5_000059"
     CDS             58868..59044
                     /gene="rpsU"
                     /locus_tag="ACF3T5_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFD3630703.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59071..59517
                     /locus_tag="ACF3T5_000060"
     CDS             59071..59517
                     /locus_tag="ACF3T5_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFD3630704.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59671..61050)
                     /gene="brnQ"
                     /locus_tag="ACF3T5_000061"
     CDS             complement(59671..61050)
                     /gene="brnQ"
                     /locus_tag="ACF3T5_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFD3630705.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
     gene            61756..62727
                     /locus_tag="ACF3T5_000062"
     CDS             61756..62727
                     /locus_tag="ACF3T5_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFD3630706.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62751..64943
                     /locus_tag="ACF3T5_000063"
     CDS             62751..64943
                     /locus_tag="ACF3T5_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFD3630707.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKVNDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64960..65436
                     /gene="ybeY"
                     /locus_tag="ACF3T5_000064"
     CDS             64960..65436
                     /gene="ybeY"
                     /locus_tag="ACF3T5_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFD3630708.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65420..65815
                     /locus_tag="ACF3T5_000065"
     CDS             65420..65815
                     /locus_tag="ACF3T5_000065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFD3630709.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65830..66729
                     /gene="era"
                     /locus_tag="ACF3T5_000066"
     CDS             65830..66729
                     /gene="era"
                     /locus_tag="ACF3T5_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFD3630710.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66872..67684
                     /gene="recO"
                     /locus_tag="ACF3T5_000067"
     CDS             66872..67684
                     /gene="recO"
                     /locus_tag="ACF3T5_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFD3630711.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68068..68985
                     /gene="glyQ"
                     /locus_tag="ACF3T5_000068"
     CDS             68068..68985
                     /gene="glyQ"
                     /locus_tag="ACF3T5_000068"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFD3630712.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68987..71062
                     /gene="glyS"
                     /locus_tag="ACF3T5_000069"
     CDS             68987..71062
                     /gene="glyS"
                     /locus_tag="ACF3T5_000069"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFD3630713.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGKASRGHRFLGEDVTFTHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23274 a        12470 c        15187 g        20167 t
ORIGIN      
        1 gggactgcga caagactttt gtctcagtcc ctttttttgc atatttttag catttatttc
       61 ctaaaaaatg taaggggatt cctatatttt taagtatact tgtttttcct tttatacatt
      121 ttatggtatt atcattttat tagttattat aaaacaatcg ttagaaagga cgtcattatg
      181 caagtatacg gaattgatgc ggtgcttcga atcgtgagtc atttggcgtt tatctactta
      241 gccttctggt ctttacgttc gttacgcatt gaaacttttt tcagaccaat gcataatacg
      301 caaattagaa tggcaatcgt acttttttca atttttttag ggtacactgc aagtaatttt
      361 ttcttagaag ttatcgcttt atgccgaaat cttttcgtta gcctgtaaat tttaaaaaaa
      421 ggtactattt taaaagcaag gatgttataa tagaaaagat tttgagtaag tgtatgctca
      481 ttggaatttt ttggagggaa caacatggaa gagatcatcg taagaggtgg caatcaatta
      541 aatggaacag taagaattga aggagctaaa aatgctgttt tgcctatttt agctgctagt
      601 ttgttagcag aagaaggtat tacgacatta gacaacgttc cgattctttc tgatgtcttc
      661 actatgaatc aagtgattcg tcacttaaac gtagatgtag attttgatga acaaaagaat
      721 caagtaacaa tcgacgcttc aagacaatta gaaatcgaag cgccttatga gtatgttagt
      781 caaatgcgtg cttcaatcgt tgtgatgggt ccgctacttg ccagaaatgg acatgcaaaa
      841 gtagctatgc ctggtggatg tgcgattggt aaacgaccaa tcgatttgca tttaaaagga
      901 tttcaagctt taggagcaaa aattattcaa aaaaacggct atatcgaagc aattgcggat
      961 gaattgatcg gtaacacgat ttatttagat ttccctagtg taggtgctac tcaaaatatc
     1021 atgatggctg ccgtaaaagc aaaaggaaca accatcattg aaaacgtagc tcgcgaacca
     1081 gaaatcgttg atttagcaaa cattttgaat aaaatgggcg ctcaagtcta tggagctggt
     1141 actgaaacaa tgcgtattga aggcgtggat catttacatg cagtcaacca ctcaatcgta
     1201 caagatcgta tcgaagcagg tacctttatg gttgctgctg caatgacaca aggcaatgtg
     1261 ctgattgcag atgctatctc tgaacataac cgcccattga tttctaaatt gatcgaaatg
     1321 ggtgcagaaa tcattgaaga agaaggcgga gtccgtgtca tcggaccaaa acatatctta
     1381 cctacagatg taaaaacgat gcctcatcct ggtttcccta cagatatgca agctcaaatg
     1441 acagctatcc aattagttgc agaaggtaca agtgtagtga ctgaaacagt gtttgaaaat
     1501 cgtttccaac atttagaaga gatgcgccgt atgaacgctc atgtcaaaat cgacggaaat
     1561 gtagcaatca tggatggaaa ccatgaattg caaggtgcag aagtatatgc aacagacttg
     1621 cgggcagcag cagcattagt tttagctggc ttgaaagcaa atggaatcac acgtgtgcgt
     1681 aacttgaatt atctagaccg tggatattat aatttccata tcaaactaca gcaattaggt
     1741 gcagacgttg aacgtgtaga catggatcaa acttctgctg aaaaaacagc acaaacgatt
     1801 gcttaaggaa gtggctgaat gagtaaccaa cgatatatct tattaacttt gataaagatt
     1861 ttaatggtga tcttattgct tatccttctt tttgtggccg gaacgatgat cggatatggt
     1921 gtcatcggtg gcggcaatcc atttaaagta tttcagccaa gtttgtggat tcatattcga
     1981 gacttttttc attgataaat aagaatcaca gtggttctag tcgatatgaa gtgagccatg
     2041 aagcagaaca tgataaaaga aaacagaagg gaacgttgac gttagtcgcg atcccttttt
     2101 ttagtaaaag aaaagaagga atctaattga tgaagaagat attgatttat ttagttagag
     2161 gatatcagcg ctttatttcg cctgcatttc cacctagctg ccgttattac ccaacctgtt
     2221 ccaattatat gataaaagca attcaaaccc atggcgcgct aaaagggacg gtaatgggga
     2281 ctgcacggat cttacgctgt catccttttg tcaaaggcgg tatcgattat gtaccattga
     2341 cattccgatt aacgcgaaat aagaaagata aaaaagaacc agattataaa aaggttgtga
     2401 aaaatacggg tagcactgag cgataaagaa acaatttgga aaatagcttc ttatattttg
     2461 caaaattgtg agttattgcc gaagtgttgg attttgaaca ccgtcaaaaa aaaggttgtg
     2521 aaaaatacgg ctagcactga gcgataaaga aaaagaggaa atttattgtc aaagtaacaa
     2581 ctccgcaact acttatatgg gggacaaaaa atgatcgaag aactaaacaa gaaagattta
     2641 ccatggtcct tgttattaga tgcagatcct gataaagaaa aagttcaagt atatgtttcc
     2701 agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa taggtgtact gatctatgtg
     2761 gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa ccccaacgta tcaaggaaag
     2821 gggattggcg gaaaattact tgagacagct tttcagaagc tttcacaact cgcttccagt
     2881 caaactcaga tcatcatacg aaccggcagt actagcagtg cggcgttaca tctctatcaa
     2941 aaaatgggct atgtggagat tggtcgagtc aaagactatt ttatccatca ttactctgag
     3001 cccatttttg aaaatggaga tcaattaagg gatcaagtga ttttagcaat tcacttacct
     3061 aaaaatagct ttaactaata aaatttcaat tcaagaaaat gctttcaaat gctaaataga
     3121 tacattacaa tagctttagc aaacaaaaag gagaatattt ttatgggaat attagttttt
     3181 gattttggtg gatcggctgt caaatatggt tgttgggatg gcaaagagat taaaggtaag
     3241 ggacaatttg caacgcctga atcttgggaa gaaatgaaag cgcaattatt tcaagtttat
     3301 aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa tcagctctcc tggagtagtg
     3361 aataatgaga aacaggtgat tgagggaatc agtgcgattc cttatattca tggatttaat
     3421 attttccgag atttggaaac tctgtttcaa cttccagtaa cgattgaaaa tgacgccaac
     3481 tgtgccggaa tggctgaatt ttatgaaggt gctgcccgcg attatcaaga tgttgctttt
     3541 gttgtggcag gaactgggat tggcggggcg ttgttcacga aaggacaaat caataaaggg
     3601 gcacatcttt acggtggaga atttgggttg atgttcttaa atggcgataa gacattcagt
     3661 aaacttggta cagcggtaca gatggcttgg cgttattgtg aacgcaaagg tctcgataaa
     3721 aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa ctggagatgc aatagcaaaa
     3781 gaagaagttg agagttttta tacgtatctt acaaagggat tattcagtat tcaattttcg
     3841 tttgatccag aagtgatcgt attaggcggc ggggtttctg caaaagaagg gctcattgat
     3901 gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg atctgcatga tttcgaaccg
     3961 cagattttat tatgtgaata cagaaacgat gctaatcttg taggtgcagc agcaaactat
     4021 ctcgctgtac gtcaagggga gaattaagag ctttagagaa ggtgcagctt gcataaaaga
     4081 agtgaatgaa atgaatagga gatttttacc taaggaatgt tttatttgct gcagtatgat
     4141 aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa agatattgaa gtgcgtatag
     4201 aagaagtgaa aaaagatatc aaaggtaaga attatgaagt cactcagtta tttataggga
     4261 aaaaaatgat tggtgagatc ttagcctatg gaccaaaaga atatgaaatt ttctttggtg
     4321 aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat agaaacagtg attcgacact
     4381 ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa taaagggttg caaaaatctg
     4441 gaaaattagg tattataact aagtcaggtt gatacaaacc tttcggagga gtagcgaagt
     4501 ggctaaacgc gacggactgt aaatccgttc cttagggttc agtggttcga atccactctc
     4561 ctccatttca ttggggtata gccaagcggt aaggcaaggg actttgactc cctcatgcgt
     4621 tggttcgaat ccagctaccc cagtatacca caatcgtaag ctctcgttag ttcgagagct
     4681 cttttttttg ctttgctacc ttgttccttg aagattctgc tgatttaaga tagagttaag
     4741 ttaactacaa gaattggagg gatccacatg aaatggaaag aaaaagtaga cgaagcagct
     4801 gaaaagttat atgatttggt caaatcagaa aaatacaata ttgaagtgaa tattccgaaa
     4861 aaaggcggaa aagcggtccg ggtaaaatca aaacgcccaa cgaatcatac aaaaaaatgg
     4921 atggctaaaa accgctaata ataacaaaag atccataagt cggcattttc atcgatttta
     4981 gggtcttttg ttattttcta ggttggacat atctaaaaaa ttgtttctga caataggagt
     5041 aggaagaacg atgtgtgtct aaatcctctg ttgatggata gaaaccttta tctttttcag
     5101 ttgtgaatca ggtatatacc agttataata gaagaaacga aaggacgggt gtacgttatg
     5161 tcgaatcaat tacctgtata tatacagatt catgaccaat taaaaaatga aatcgaacaa
     5221 ggtatatgga aagtcggaga ccgtcttcct tcagaaagag aattagcagt gaaattcaat
     5281 gtcagccgta tgacgttgag acaagcaatt caaaacttgg cagatgaagg aatcttagaa
     5341 agaaagatcg gttcgggtac atatgttgca cgagaaaaag tacaagaaaa aatgtctggt
     5401 gcaacaagtt ttacagaaat tatggaatca caaaatcgag taccgtctag tcggacaatc
     5461 tcttatttct tgacttcgcc aagctcaagt gaaatggaaa aactaaactt gagtaaagat
     5521 gacacgatct tgcgaatgga acgtattcgt ttcgcagatg atgtccctat ttgttttgaa
     5581 gttgccagtg tgccacaaaa gctgatcgat ggctacagca aagcagagat tactgaatcg
     5641 ttgtatcgca cgctagaaga aaaaggcggg aaaaagatcg gtccagcgaa tcaaacgatt
     5701 actgctgtgt tggcttctga gaaaattgct gactatttag aggtaaaacg aggagatgca
     5761 gttcttcgtt tgcgccagat ttcttatttt gaagacggta cgccgtttga gtatgtgcgt
     5821 acgcaatacg taggaaatcg ttttgagttt tatttggaaa aataaaaaac aggtgatccc
     5881 tgattccgta ttagaaataa aagagaatca gaatcacctg tttttatata tgatcagtta
     5941 ccagttgctc gtgataagaa acaggaccct cttcaaattg gacttgtccg ttcagcagct
     6001 caatgatgga ttctttaaag tggtcagcac ttttttcatc tacagcacag atgacgctaa
     6061 cttgatcagt atattggatt tcttttataa aaaaatgttg gagttcaaga taattctgta
     6121 attttcctag ttgcggatac gtgatcatca cagagatctc ctgttgcaag gtaccttcta
     6181 cgattccaat gtcagacaat gcttgagaga ccgcatgagt gtatgctcgt attaatccgc
     6241 ctgtgccgag tttcgttcct ccaaaatatc gagtgactac tgcagctaca ttgatcagtt
     6301 gatttttttt gaggacttcc aacatcggta tgcccgccgt tccacttggt tctccatcat
     6361 cgctgcttcg ctggatttca tttttttctc caatgatgaa ggcgctgcag ttatggttcg
     6421 ctttccaatg ttcttttttt atttcttgaa tgaaactttt tgcttcgtcc tctgattcta
     6481 ttcgtctaag agaacagata aagcgtgatt ttttgatctc gatctcactt tgtccatttt
     6541 cttttattgt acgatagcgc tcgatcatct tgtcacctct tttttcattt tctttattca
     6601 gtataaataa agttctaggt tgtttcaaca ctgtaacggt tgcgcaattg ctgtagtatg
     6661 cgataataaa gacaaaggca ggtggatctg gggatgcagg aacgaatgaa aaagtacgat
     6721 gcaattacgc attatttgaa gaacaatggc ggctctcaag taacgttgac ctttacccaa
     6781 tttgacgaac tgctgtttcc cagtaacgga cttcccaaaa cagcaaggga aagtacagat
     6841 tggtgggcaa atgattataa acaccctgaa aaaggtgcct atggctggat aaatgcaggc
     6901 tatgaagtag ttgtcatcaa tttggacaaa gaatatgtcg tgttcaataa attagtgaaa
     6961 tctagttggc tatttgatta aataaaccgc gtttagaaaa cgttgacaaa tttctttctt
     7021 tttatataat agagtaacag acgcgaagaa aacgtttgcg tagtttgttt taaaaaggag
     7081 agaaaagtat ggctaaaaaa acaatcatgc ttgtatgttc ggctggtatg agcacaagtt
     7141 tactagtaac aaaaatgcaa aaagctgctg aagcaaaagg aatcgactct gatattttcg
     7201 ctgtgtccgc ttctgacgca gataacaatc ttgcaaataa agacgtggat gtgttgttat
     7261 taggcccaca agttcgcttt atgaaagctg attttgagaa acgtctggaa ccaaaaggca
     7321 ttcctttaga tgtcatcaat atggctgatt atggtatgat gaatggcgaa aaagtgttag
     7381 atcaagcaat cacgctcata ggtgaataaa tagaacagta taactatcga acaagcgatc
     7441 caaaaaatag atcgcttgtt ttttcgtata tttttttagg gcgaataata tgaatcattt
     7501 ttggggtaga aggcttctga cgagagatat taaagcaata gactgtgagg aggtaaattt
     7561 accaaattat gagaagatcg cagcagtgga acgtttgagc aactggattc tttgtcatcg
     7621 ctgtggaatg agaacgtccg tcattgatgg acagcttgca gattacggtt atttttgtac
     7681 acattgtcta tatttagggc gatgcgacac tcaacaagat ttatatttgt ttgatcagcc
     7741 tgaagcaaaa cctcgagaag tcgtcttttt tttggacagg acagcttact gaaaaacaaa
     7801 cggaaatctc aaagagaata ctttgccatt cagaaaaaag acatcatctt atttgggctg
     7861 taacaggtgc agggaaaaca gagatgcttt atcctatttt agtggaaact ttaaaagctg
     7921 gaggaagagt agcgatttgt acaccgcgag tcgatgtttg taatgaattg tttttacgct
     7981 atcgttatgt ttttccagaa gaaaaaatca cgttgctcca tgggaataca gcaacggctt
     8041 atacctttac ttcttttgtt atctgtacga ttcatcagct ataccgtttt tatcggtcct
     8101 ttgatttaat agtgatcgat gagatcgatg cgtttcctta taacggggat gcaggattag
     8161 ctcatgctgt gcaaacagcg attaaaccag acggacgatt catctactta agtgccacac
     8221 ctgataaaaa gttactaaag gagatcaagc agacgtttga catccacaaa cttgctttac
     8281 gctttcatcg gcgtttgctt cctgaacccg tcttggtttt catgaataat tggcgacaaa
     8341 aatgcaagaa caagaagaag ataaaatctt tggtgcggat cattcatcaa ttaattaaag
     8401 aaaaccatat ccttttattt tgtccaagta tcacgatgat gcatcgtttg aaagccacac
     8461 ttcaagaggc tcttccgaga tatgagatca cagatgtttc ttcaagagac aatcagcggg
     8521 cggaaaaagt ccagaaaatg cgagaagcaa aatatgacat actgttaacc acgatgatcc
     8581 ttgaacgtgg agtcacattt gaaagaatat ccgttatcgt tttaggagcg gatcatccgg
     8641 tttttactaa atcttctttg gtacaaatcg caggaagagc cgatcgtaaa gggccttata
     8701 cgaacagtca agtatatttt ttctatgaag aaaaaactgc agctattcga aaagcttgtc
     8761 aggaaatcaa agaaatgaat gaagaaggga agaagatact gtgaggtgca gttgctgcag
     8821 taaagatatc gtccaaaatt taacattcag cgagattatt ggtgtttcga aatacagatt
     8881 acctcagact tgttcagttt gtcgcgcaca atttcattta ttggaaacaa gaggctgttc
     8941 tggctgtcaa aaaccaatcc atttcaatct agagagtacg aaaaagaaag taaattctat
     9001 ccccgtttac tgcagtgact gtcaagaatg gaagaagaaa tatccgacct atgattttca
     9061 gcaccgtgcc ttttttgtgt acgatgaaaa tatgaaggcc tggctcaatc aatacaagtt
     9121 tatgggagat atccgactag caggcacctt tgcatataaa tggacgcaga taaaaaaaga
     9181 atacccgaca tacatctatt gtccaatacc tttatctgaa aaaaggcgaa cagaaagagg
     9241 attcaatcaa gtaagcgaga tgttgaaagc agcaagaatt ccttttcaga tgctactcaa
     9301 aagagagaag catctgctgg ctcaagcaaa aaacacgcga aaggaaagat tggagatgcc
     9361 tcaacctttt gaacttgcag tcaccttttc taagatacaa aataaaaaaa tattgattat
     9421 tgatgatgtc tatacaacag gacagactct tttccacgca gcagattgtt tgcagatcgc
     9481 acagccgaaa atcatccgaa ctatttcttt ggcgagatga attagatgtg gaaatttgat
     9541 agcgatttaa tttgcaaata acctagagtt cgatataata gaattaagaa gagggaaaga
     9601 gtggtccttc tccttcttga ttgtggtaga cgaaaggggt aatttttatg tttagatata
     9661 atgtacgcgg cgaaaatatc gaagtaactg aagcgattcg cgactacgtt gagaaaaaag
     9721 taggcaaact agaacgctat ttcagtgacg cacccgatgc aacggcgcat gtgaacttaa
     9781 aagtttatac agaaaaaaca gcaaaagtgg aagtaacgat cccattacct tatcttgtat
     9841 tacgtgccga agaaacttct ccagatttat acgcaagtat cgatttggtt gttgacaaat
     9901 tggaacgcca aatccgcaaa ttcaaaacaa aaattaatcg taaatcccgt gaaacgggca
     9961 tgaatactgc aaatgctgct gctttattca atgaagagga cgaaaacgaa aatgattctg
    10021 aattagatat cgtacgtacg aaacgccttt cattgaaacc aatggatagt gaagaagcag
    10081 tcttgcaaat gaacatgctg ggacataact tctttatttt tgaagatgca gaaacaaatg
    10141 gtacaagtat cgtttaccgc cgtaaagatg gaaaatacgg actgattgaa acagactgat
    10201 cagccttact taaataacaa cgaaaacttg cagcagctac tgacagctgc cgcaagtttt
    10261 tttgagtttc ctcctctttt agatgaaata tgaataaatt atgaagttct tactttaata
    10321 cgcttaagat atttcaatta tctataacta atgataaaat gaaaagtaaa gtctacatag
    10381 tctggataat acacagaagg aaaaggagat tacacaacgc atggccaatt tgattcgttc
    10441 acttatcgaa aacgataaga aagaactaaa acgtttagat agcattgcaa aaaaagttga
    10501 agcatacgct gaccaaatgg cagcattaac tgatgaacaa ttacaggcaa aaactgctga
    10561 attcaagggc agataccaaa aaggagaaac attagatcaa ttacttccgg aagcttttgc
    10621 agtcgtccga gaagcagcaa aacgtgtatt aggtttatat ccataccacg tacaattgat
    10681 gggtggaatc gtgcttcacg atggaaatat ccctgaaatg cgtactggtg aaggtaaaac
    10741 cttgaccgca acgatgccag tatatctaaa tgctctgact ggcgaagggg tacacgtagt
    10801 taccgtcaat gaatatttag caacccgtga ctcgacggaa atgggcgaac tttataattt
    10861 cttaggatta acagtggggt tgaatatcaa ctcaaaatct tcggatgaaa aacgcgaggc
    10921 ttataattgt gatatcactt atagtacgaa taatgaatta ggatttgact acttgagaga
    10981 caatatggtc gtttaccgcc atcaaatggt acaacgtccg ctgaattatg ccattgtcga
    11041 tgaagttgac tcaatcttga ttgatgaagc gagaacgccg ctgatcattt cagggcaagc
    11101 agaaaaatcc acagctttat atacaagagc agacaacttc gtgaaacgtt tgaaagaaga
    11161 agaagattat aaaatcgatg tacaatcaaa aacaatcagt ttaaccgaag cagggatcga
    11221 aaaagcagaa gaaacattcg gtttggataa cttatacgac attgaaaata ctgcattgac
    11281 ccatcacttg gatcaagcat tgagagccaa ctatatcatg cttttagata tcgattatgt
    11341 tgtccaagac ggaaaagtac tgattgttga ccagttcact ggtcggatca tggatggacg
    11401 ccgttattca gatggacttc accaagcaat cgaagctaaa gaaggcgtag agatcgaaga
    11461 cgaaacgaaa accatggcga cgatcacttt ccagaactat ttccgtatgt acaagaaact
    11521 tgcgggaatg actggtacag ctaaaacaga agaagaagaa ttcagagaaa tttataacat
    11581 ccaagtatac cagattccga caaatcgtcc ggttatccgt gatgatcgtc cagatttgct
    11641 ttacccaaca ttacaaagca agttccacgc tgtcgtgcaa gatatcaaag atcgctaccg
    11701 caaaggtcaa cctgtgttag tcggaactgt tgcggttgaa acatctgagt tattatcaga
    11761 tatgctgaat caagaacgta ttcctcatga agtgctgaat gcgaaaaatc actttaaaga
    11821 agcagagatc atcatgaatg ctggtcaaaa aggtgctgtg acgattgcta ccaacatggc
    11881 tggtcgggga acagatatca agttaggact tggggtaaga gaacttggtg gtctagcagt
    11941 tatcgggaca gaacgacatg aatctcgtcg tatcgataac cagcttcgag gacgtgcagg
    12001 acgtcaagga gaccctggta tgtcacagtt ctacttgtca ttggaagatg atttgatgaa
    12061 acgattcggt tcagaacgaa tcaaatcttt cttagatcgg atgaagatcg gtgaggaaga
    12121 tgctgttatc caaagcaaga tgctgacgaa acaagtagaa tctgcacaaa aacgtgtaga
    12181 aggaaataac tatgacacac gtaaaaacgt cttacaatac gatgatgtga tgcgtgaaca
    12241 acgtgaagta atctataaac agcgacaaga agtaatcatg gaagaaaaaa gcttatccaa
    12301 gccactaatg aacatggtca aacggacaat cagtcgtgta gtagatgcgc atacacaatt
    12361 agagaaagaa aattggaatt tggaaagtat catcgatttt gccggcagta ctcttgtcca
    12421 tgaagatagt attactcttg cagatatcga aggaaaaaca gctcaggaga tcaaagagta
    12481 tctaaacgaa cgagcacaag aagtatttaa taccaaagcg gctcaactaa atggtccaga
    12541 gcaactgttg gaatttgaaa aagtcgtgat cttgcgtgtt gtggactcta aatggacaga
    12601 ccatattgat gcaatggatc aattacgtca gtcaatcggc ttacgtgctt atggacagaa
    12661 caatccatta gtcgaatatc aaacagaagg ctataagatg tttgaagata tggtaggcgc
    12721 catcgagtat gaagtcactc gaatctttat gaaagctgag atccgtcaaa acgtacagcg
    12781 tgaacaggtt tctcaaggaa cagcttctca ctcagaagac ggagatgcca aagaaaatag
    12841 taatactagc gcgaaaaaac agccaatcca tgtgaacaag gtcggaagaa atgatccttg
    12901 tccttgtgga agcgggaaaa aatataagaa ctgtcatgga aaaaatgcaa actaatttct
    12961 gacagcagga ttggtgagac aaattctttt tttaagaatt cgtctcacct tctttctgtt
    13021 atttgctgat tctgttagac tagatataat gaaaaaagta tagtgaaaga aggaaaaaac
    13081 atggaaatat ccgaaattcg aaatctcctg acagaaatgc aggaaaaggt cacaagcttc
    13141 aggaggtctc tttgacttag accagctgga agaagacatt gcggaagcag aagcaaaaat
    13201 ggctgaacct ggtttttggg atgatagcga agctgctcaa aaagtgatca gtgaaaacaa
    13261 tgcaaataaa gaaacttatg atcaattcca ttctcttgct gaagaactgg aagaaatcga
    13321 attgatggtg gagatgttgc aggaagaacc tgatgcagcc atgcaagaag aagcagaaac
    13381 acgtatcaat gcgctagatg aaaaaatgaa gacatatgaa ttatctatgt tgttagatgg
    13441 tccttatgat cgaaacaacg ccatcgttga attgcatcca ggggctggcg gaacggagtc
    13501 ccaagattgg ggcagtatgc ttttacgtat gtatacacgc tgggcagagc agcatgggta
    13561 tcaagtagag acactagatt atcaagcagg tgatgaagca ggaatcaaga gtgtgaccct
    13621 actgatcaaa ggacacaacg catttggtta tctgaaatca gaaaaaggtg ttcatcgcct
    13681 tgtacgtatc tcgccttttg actcagcgaa acgacgtcat acttcttttt gttcggtaga
    13741 tgtgatgcca gagcttgatg aaacgatcga tatagaaatc aatccagatg atttgaaaat
    13801 cgatacgtac cgagcaagtg gtgcaggtgg tcagcacatc aataaaacag aatctgctgt
    13861 acggatcact cacttgccta caggaacagt agtagctagt caggcacaac gttcacaatt
    13921 gaaaaaccgt gagcaagcga tggggatgtt aaaagctaag ctttatcaat tagaagtgga
    13981 aaagaaagaa caagaagcag ctgcacttcg aggagaacaa aaagaaatcg gctggggttc
    14041 acagatccgt tcttatgttt tccatccata ttctatggta aaagaccatc ggacaaacgt
    14101 agagacagga aatgtccaag cagttatgga cggtgacctt gatttattta ttgacggcta
    14161 tttaaaaatg catctacaat aagaacccat tgttaaattg aaataaaatt gtaagatttt
    14221 gcaagcatgt tgaaacattg ccatgctata atagctaaag actgaaaaag atatggagag
    14281 aacgccatga ttgaaatgaa ggatgtaatg aagaagtact caaatggtac gaccgctatc
    14341 cgcaatctct caattgtcat cgaacaagga gagtttgtct atgtggttgg tccgtctgga
    14401 gccggtaaat ctacattcat caaattgatg tatcgagaag aaaaagcttc aaaaggaact
    14461 ttgaacgtcg caggtcatga tctaatagca atcaaaaacc gcgaagttcc ttatctacgc
    14521 agagaaatcg gcgtggtgtt ccaagactat aaattattac caagaaaaac agtatatgaa
    14581 aatgttgcgt acgctatgca agttatcggc agaaaacctc gggatatcaa aaaacgagta
    14641 atggaagtgc tggaccttgt tggtttgaaa cacaaagttc gagtctttcc tagcgaatta
    14701 tccggtgggg aacagcaacg tgtctctatt gctcgggcaa tagtgaatac gcctaaagta
    14761 cttatcgcgg atgaaccgac aggaaatctg gatccagaaa actcatggga aatcatgaaa
    14821 ttattagatc ggatcaatgc ccaaggaact actgtcgtga tggctacaca taacagtacg
    14881 atcgtaaata cgattcggca ccgagtaatt gctatcgaaa acggccgcat tattcgagat
    14941 caagcggaag gggaatatgg atacgatgat tagaaccttt ttccgccatt tattagaaag
    15001 tatcaagagt ttgaaacgta acggctggat gacagttgct tcagtcagtg cggtaacgat
    15061 cacattgact ttagtcggtg tattcatggc agttatcatg aacgcaacta aattagctca
    15121 agatatagaa ggaaacgttg atgtctctgt attcgtagat atcggtacaa aagacgaaga
    15181 gatgaaaaag ctagaaaaag aattgaatga cttagatcat gttaaaaaag ttgaattttc
    15241 aagcaaagaa caggaattga aaaaaatcca aaacgaaatg ggtgattcct ggaaattgtt
    15301 tgaaggtgac aataacccgt tatatgatgt gtatgtagta agtgctactg atccttctta
    15361 tacaaaacaa gtagcagaag aagcagcaga tttgaagaat gtttctcgtt ctgattacgg
    15421 tggagcttcc tcagatcgga ttttccaaat cgctggtgca gttcggacat ggggattagt
    15481 tgcagcggca ttgctgctgt tcgtagcgat gtttctcatt tcgaacacga ttcggatcac
    15541 gattttgtct agacagcgtg aaattcaaat catgcgttta gtcggtgcaa aaaatggata
    15601 tattcgctgg ccgtttttcc ttgaaggtgg ctggatcggt ctgatcggtg cgattcttcc
    15661 gattttgatc atcacatttg gttatgcatg gtttttccaa ttgatcaatc catcattatt
    15721 acgttcccat tattcattga ttcatccaca aaatatcgtt tggaaaatca atcttttaat
    15781 ggtcggcatt ggtgtgatca ttggttcact tggctcgatc atttctatga gacgtttctt
    15841 gaaattctaa taccacaata taaataaaat gagaaagttg gacagtttcg ttccagcttt
    15901 ctttttttat tggctaggag aatagaagga agcggctttt ttccctttac tttaaaaaac
    15961 aacttgggta ttcttaagtt ggtagaagaa gaggtgaagc cgtgaaaatt atcgttgaca
    16021 atttttttac gatccttttg attatgaata ttttgctttc ctttatcatc gtttttcgag
    16081 aaagaaagga aactgctcaa acatgggcat gggtgctcgt tttgatgttc attccagttg
    16141 taggatttat tttatatatt tttttaggaa gagggatttc caaagagaaa atctttgatt
    16201 tgaagatcca agggaaaatc ggtaagaacc tggaaattga agaagacaaa caggcattga
    16261 tgcgagggct ttatcctcat ccgcctactg gacaagtaga tgtgaaacaa ttgatctata
    16321 tgctcacggt atttgaaagt acgctctata cgactaaaaa tgaaatcact ttatttacag
    16381 atggtcgtga aaaattcgat gcgctgattg aagatatcaa gcaggcagaa gaccatatcc
    16441 acttccagta ttatatttat cgcagtgatg cattaggtga agaagttcga gatgcattga
    16501 cagaggcggc aagacgaggg gtaaaagtca gagtccttct tgatgcatgg ggttccacac
    16561 aagtttcttc cagctttttt cagaatttga aaaaagcagg cggagaagtc gcatttttct
    16621 ttcctctatt tgttccgtat attaatcctc ggatcaatta tcgcaatcat cgtaaaatcg
    16681 tagttattga tgggaaaatc ggatatacag gtgggttcaa tgttggaaat gaatatctag
    16741 gattggtaaa gaaatttggt tattggcgag acaatcattt gcgtatttac ggagaagcgg
    16801 tgtatacctt gcaaaaccgt tttttgatgg actggaattc tcagcataca aaagaagtag
    16861 gctattctcc caaatttttt ccgtccattc actctactgg tgatatagct gttcaaatcg
    16921 tgacaagcgg accagatacc gaacatgaac aaatcaaaat gacttatttg aaaatgattt
    16981 cgttagccaa acgggagata ttgatccaaa cgccttatta cataccggat ggatcgattc
    17041 atgaagcact gaagttggct cttctttcag gcgtaaaagt tcatatccaa atacctaaca
    17101 aaccagatca tcttctcgtt tactgggcaa cgtattcttt cgctgcagag ctaatcgagt
    17161 atggagcaag gatcgaaaca tatgagaatg gatttattca tgcaaagacg atgattatcg
    17221 atggtggcat tgtttcagtt ggctcagcaa atatcgatgt tcgttcattt cgcttggact
    17281 ttgaggtcaa tacactggtt tatgatgaac gaatggcttc acgtgtaaga aaagcttttt
    17341 ttgaagactc gaaaatttct acgcatctca caaaagaaat gtatgaaaac cgaggaataa
    17401 tcatcaagat gaaagaagga ttagcaagat tgatttcgcc gctcctttaa aacataaaaa
    17461 ggacatgaga accatcaaca gatggagatc tcgtgtcctt ttctaacggg gccatttctg
    17521 agagtacgtc agaaatggag aaagcgtctc gcatgagcga aattaacttt ggggtatctc
    17581 ttgttttttg gggaaagcaa gaaataagct aatatatgct attaaaagag acacatttca
    17641 aagccagtat agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta
    17701 ttttgttatt taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca
    17761 tactttagga ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag
    17821 gattagctat tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg
    17881 ctgttggctc ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag
    17941 aaaatcctgg aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag
    18001 tacagtcagg agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga
    18061 tcgatgcctc agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga
    18121 acaaggatat tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg
    18181 agatcaaaaa ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg
    18241 cttcaggaag cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg
    18301 ttcaggcaca agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg
    18361 gtgcaatcag ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta
    18421 acggtgtaga accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg
    18481 agcatatgta cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct
    18541 tatctgatga agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata
    18601 tgaaagtcga acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt
    18661 acaaacttag ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact
    18721 gagtgagtat gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat
    18781 tattaacaaa atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat
    18841 gtatcgcgct gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat
    18901 tggccacctt ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga
    18961 atccaagcca aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag
    19021 gttcgtttat tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag
    19081 cagtattcat gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg
    19141 agctgcttgt aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc
    19201 cttttgtcag aagtattttt ggcgggactg ggtttggtat cttagctggt acatttgtgt
    19261 tgttcgttat gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc
    19321 cacggcacta tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca
    19381 aagtcgtgct acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc
    19441 gtgcgtttgg tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa
    19501 caggtcttac tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata
    19561 caatcatggg aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga
    19621 tgtcattatt ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat
    19681 gaacgcaaaa cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat
    19741 cgtattgatt ttagcagctt tactacttta tatcttagtt cggggaatac cgcacatttc
    19801 atggtccttc ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca
    19861 gctatttaac tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt
    19921 aggtgcgggt atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg
    19981 cacgtcgatt gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt
    20041 ggttttcgtt attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac
    20101 tgtattcaac ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta
    20161 cacacaacga gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt
    20221 ggtggttcca gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat
    20281 ttttggtgaa gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt
    20341 tacgaactgg aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac
    20401 attagctgtc catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt
    20461 ttcggctggc gcttctgccg tgttgattat tgctgtttta ctgttcaact tcggcgctcg
    20521 tgcgattggc aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg
    20581 aaaaaatata actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca
    20641 ctatatacgg atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat
    20701 ctggaatttg aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct
    20761 acgtatcttc gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg
    20821 aaaatcatgt atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc
    20881 aaacggattg gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat
    20941 attacatttg ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt
    21001 gaaacaagct tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt
    21061 gccttggctt tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg
    21121 aaacctgata tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga
    21181 acggttgaag aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat
    21241 aacatgcaac aagcagctcg tatcagcgac tatactgcct ttttctattt aggaaaagta
    21301 atggaatatg acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat
    21361 tatgtgtccg gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga gattatttca
    21421 tctaaagatc ttcatctgta ttatggaaag aaagaagcac taaaagggat cgatctaagc
    21481 ttcaatcaag gggaaatcac tgcgatgatc ggtccttctg gttgcggaaa atccacttat
    21541 ctgcgttctt tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt
    21601 ttatataaag acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa
    21661 atcggcatgg tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc
    21721 tatggattga aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa
    21781 agcctgaagg cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta
    21841 tctttatccg gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg
    21901 gaaatcatct tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc
    21961 gaaagtatgc tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg
    22021 tctcaagctt cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa
    22081 tacaatgata cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt
    22141 tcaggtcgat ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg
    22201 ctgaatctcc ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag
    22261 tcagttcgtg catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat
    22321 ataaatatca ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg
    22381 caacagccag ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat
    22441 ttagaacgta tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg
    22501 cagacgagaa taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa
    22561 atggtggaca acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc
    22621 gccaatatgg atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa
    22681 aatatgcagg aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt
    22741 tatctagaac ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct
    22801 acagggaaaa tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg
    22861 attatgccgt ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct
    22921 gcatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    22981 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    23041 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    23101 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    23161 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    23221 ttgattacag gaaaactgat gaacacggat caaaaaggag aatcgattta tttatcggtt
    23281 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    23341 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    23401 ttcacaagta tcttcattgg cgccatgttg cttattggtg gaaaacaggg tctatttgct
    23461 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    23521 acagaaggga tttctctatt aaatatatct atcctgttcg tcgttatagg gatgattatt
    23581 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    23641 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    23701 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    23761 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    23821 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    23881 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    23941 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcgtt caaagaaacg
    24001 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    24061 ttatcgatcc ctatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    24121 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    24181 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    24241 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    24301 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    24361 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcaatcc
    24421 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    24481 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    24541 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    24601 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    24661 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    24721 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    24781 ctattcttac cacattgtcc gcactacttg ttcttccttt aacctgcgtg actacagcat
    24841 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    24901 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    24961 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    25021 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    25081 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagatcagg
    25141 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    25201 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    25261 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    25321 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    25381 gcgagttgac agaagaagaa ttagcacagc gtcagacgtt agaagcagaa atcaaagaat
    25441 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    25501 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    25561 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    25621 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    25681 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    25741 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    25801 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    25861 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    25921 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    25981 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    26041 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    26101 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    26161 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    26221 ttggtgcgac agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta cgtcttacga
    26281 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    26341 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaaca
    26401 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    26461 aattccgtcg tgtttcagat ggtgaaatcg cgcaagtgca actttcaacg acaacaggaa
    26521 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    26581 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    26641 gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa ttatcgccga
    26701 gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt aatttgtgac
    26761 cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat tttcttcatt
    26821 taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ctaataaaaa
    26881 tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt
    26941 tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa ttattttata
    27001 cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa
    27061 caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg
    27121 caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa
    27181 cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc
    27241 catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg
    27301 tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg
    27361 tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa
    27421 cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc
    27481 aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg
    27541 cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa
    27601 acgttttttt taattggtag tacttttggc tagacaatga taaaatgata gagaacactc
    27661 taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa
    27721 attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg
    27781 ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg
    27841 tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg
    27901 tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg
    27961 attaccagct ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg
    28021 ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct
    28081 tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat
    28141 tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggcca
    28201 tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg
    28261 agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga tcattgatgt
    28321 aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta
    28381 tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt
    28441 tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt
    28501 tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc
    28561 aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta
    28621 gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt
    28681 cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat gtggcttagc
    28741 agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg
    28801 ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga tcttccccag
    28861 tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc
    28921 ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg
    28981 tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg
    29041 ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt
    29101 ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca
    29161 acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga
    29221 aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt
    29281 ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc
    29341 cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg
    29401 cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt
    29461 atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag
    29521 agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa
    29581 cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca
    29641 acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac
    29701 caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc
    29761 aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca
    29821 tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga
    29881 aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga
    29941 agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa
    30001 atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat
    30061 cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca
    30121 cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat
    30181 cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggaat
    30241 aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt
    30301 gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg
    30361 tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg aaatgcccat
    30421 cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga
    30481 aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt tttgaaggag
    30541 cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg aacaagattt
    30601 ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc
    30661 cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat cgttacagga
    30721 aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta
    30781 agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg
    30841 catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc
    30901 tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga agacgaagta
    30961 ccattgacac agcaattaat cgatgattac gaacgtactc atgcttctac gatcgccgtt
    31021 atgcaagttc ctcatgatga aacttcaaaa tacggaatta tcaatccagg agaagtattg
    31081 gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca
    31141 agtgatttag ctattattgg acgttactta ttgactccag aaatttttga tgtattgtcc
    31201 gagcaacaac caggagccgg aaatgaaatc caactaactg acgcaatcga tacattgaat
    31261 aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt cggtgacaaa
    31321 tttggtttta tgaaaacaag tattgaatat ggtctaacac atccagaagt aggtgcacct
    31381 ttacgtgagt acatcctgtc tttaggtaaa acattagaag cagaaggaag caagatcgat
    31441 cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataacc cttttttcta
    31501 acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa
    31561 aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg
    31621 aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg tttttatcgt
    31681 tcgtgtgttg ttgcaagttt ctaaaacttt agaaaaagta gatcagactg tagcagaagc
    31741 aaatacgacg atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac aagtagaagg
    31801 cttactggtc aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg caacgatcga
    31861 tccattgttc acagcagttg ctgatttaag tgaaagtgta tcagaactta atcattctag
    31921 taaaaacatc gcgacgaaag tcggcggaat cggtaaaacg acagctaaag cgacggttgc
    31981 cggaaaagtc ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac
    32041 aggaggaaaa taaaaatggc taaaaaaggt ggatttttat taggtgcagt agtcggaggc
    32101 acagccgcag caatcgccgc gttattgtta gcaccaaaat ctggtaaaga acttcgggat
    32161 gaattagctg cccaagcgga tgatttgatg gatatggctt cagattacac agatatggcg
    32221 aaagaaaaat cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa
    32281 caagcaggtg atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca
    32341 ggaagtgaat ctgacgatat cttgttaaat cttaaagaac aatcagctga cttatctaat
    32401 cgtttcaaaa aaacggcaga agatatgaaa aaacctgcag atgaattagg aagtattgct
    32461 aaagatactt cagaagatat catgattgat gtaaaagacg ctgctggtaa agcaggagat
    32521 actgtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgactcaaa agaaatgatg
    32581 aaaatggcaa aagaagatgc gaaagaagct aaaaaagaaa taaaagatac aatgacagat
    32641 tcatctacaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    32701 ctcacctttt ttactcttat ttttctaaaa caagtaattc ttttggtgta gtcgtgaatg
    32761 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    32821 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    32881 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    32941 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    33001 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    33061 tgtagatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    33121 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    33181 tcgtatttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    33241 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcaatttct gcaatgatcg
    33301 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    33361 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    33421 ggatgatgga agttagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    33481 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    33541 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    33601 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    33661 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    33721 atccggaaaa gtaagcgatg tggccagggt cgctgatata agacaattcg acttggtgtt
    33781 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    33841 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    33901 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    33961 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    34021 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    34081 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgttaa accagcaaca
    34141 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    34201 ggcttggcaa gtaagaaaac aacaacagtt ggtgtaatca tccctgatgt cagcaatatg
    34261 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    34321 atcttagcaa attcagatgg caacgaccaa aaagaagtca atgtattgaa taacttactg
    34381 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    34441 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    34501 gtaggtagcg taaacattga ttatgtatcc gcaacaaaag atgcagtaaa caaattggca
    34561 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc catcaatgga
    34621 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    34681 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    34741 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    34801 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    34861 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    34921 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    34981 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    35041 gacaaccatt cagaggttgt gtgcctgccg tttaactctg cgttacaagg agcaatttca
    35101 aaaaacagca tctcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    35161 caccgtttat ttgggaaaag gggttatgac aaaattttgt tataatcctt tttttgtgtc
    35221 ttaaaaaaga gagaaagtgg ttgaaattac atttatcgta tataaaacaa tccgtgtata
    35281 gatgctgcat tttgttcacc accatcaaaa aaataaggga ctgtgacaat actattgtca
    35341 caatccctta ttactgtgta ttctattaaa tcaatcatct ttctcatctg attctttagt
    35401 tgcttgattc gctcttgcat aagctgaagc ggtgttccag taatctgtat agttttcatc
    35461 ggtaccgcca acgccaaatt taaaggcgct tttcttcggt ccatttttcg cccatagcga
    35521 ttcaactgtt tttcctggtg tttggatgga ttttttattg acagtaactt ttccaggtgt
    35581 ttgaccagta aatccagata cttttttctt catcacatca gatgataatt cgaatttctc
    35641 atcagtgcca aaaatgtcag gttttgcttg gtaagcttga ttgatcagat tggcaagata
    35701 attagccgaa cgtatccttg ctttgctgtc cattccagta ttgtcatcgt gccctgccca
    35761 gctactgatg gtaatagacg gagtagagac aattagccat gaatctcggt attcattggt
    35821 agaaccagtt tttccgaccc aatctgcttt tcctagattt ccgtttaagc ttgagatggc
    35881 atctttgaat ggtgtagtga tttttgcatt gatcacacta cgcatcatgt cattcatgat
    35941 cgatgccgtt gcttccgaat agatccggac agagttgctt tcgtgtttat aaattacatt
    36001 tcctgcgtta tctgtgatgg aatcgatgat atagccttct tcatagacgc cgccgtttgc
    36061 taaagcctga aatccgttgg tctgttgaag cgtcgtgaca tctactgtac ctaaaggagc
    36121 agattcaacg ccccaattgt catttgccgg gtagttcatc tttgccaagt agtgctcgta
    36181 agcatattgt ttcgatccac cggaattcaa cacatcttga tataagtgat aagccgggat
    36241 attgttggac cattctagtg actcacggac ggtttggaag gtgttcgatc cttcgttagt
    36301 agcattgacg attttctcgc cagcattttc tccttcttgc caagttgttg cataatctga
    36361 tacacgggat tctgatccga taagccccat gtcgattgca ggtccataaa caagaactgg
    36421 tttgattgat gagccagctt gacgttctgt atcgaaggca tggttatttt gattctgact
    36481 gtaattccgt ccgccaacga atccgtagat gcggccagtt ctgttatcca tcaatacatt
    36541 tccggtttct acttgactgc cagcaccatc atctaacatg tatccataat tttcaacgcc
    36601 attttgcaat gcagcataga catctttatc gattgtcgaa tggattgtat atcctttatt
    36661 ttggatcgtt tgctgtgcct tttgataata agcgtcactg accgatgaat cagacatatc
    36721 agcgttgtct ttttcagcca attgttgtgc aatgattctt gttgcttcat tcatcacagt
    36781 ataatacaag aattctctgt cagtctgttc tgcgatttgt tgtggtagaa agtcttttgt
    36841 aaggtcatag gcttttgctt cttcgtattg ttctttcgta atttggcctt cacgatacat
    36901 actgaataaa acaaaatctt ttctggcaag gccggcagaa agatcttctt ttagcgcgcc
    36961 tgtgttcgtg tatggactat acgtgatcgg gctttggggt aatccagcga tataagcagc
    37021 ttgcggcagc gtcacatctt ttgcatctac accaaagatt ccttttgcag cttcctggac
    37081 tccagcaatg ttttgtccct tattattacg accaaatggt gagacattca aatacgtagc
    37141 aatgatctca tcttttgaaa agtatttttc tacttgtgca gaaagaatga tctcgtttgc
    37201 tttacgtttg aatgttgttt catctgtaag gatctgctgt ttgactaatt gctgtgtcaa
    37261 agttgatcca ccagaagagc caataccagt agcttcagat actaatgctc gtaaaacagc
    37321 tttaggaacg taaccttggt gtttatcgaa gtattcatcc tctgtgctga taattgcagt
    37381 tttcaataaa ggtgaaatct tatcagatga aatagtcgtg cgcatcagat ctgtctggat
    37441 ttttgatatc tctgtattat ctgcataaac aatctttgac gtttctgtaa tgtttcctag
    37501 ttctgtctgc agtgcttttt tagtcggcaa ttgtgtgtct tcgacgagat aggcaaaata
    37561 gccagcgcct attccaatac ctagagctgc gaacatcaaa agaagaacag taataaaaac
    37621 agtcaatgat tgaaaaactc taaaaataat cttaggaaca aaccattttt ctttttttct
    37681 tctattctgt ttttttcgag tatttttgcg tggcaaaagg gtcacctcaa ctaatggttt
    37741 tatttcgcta attatagcaa ataatagaac aaaaaaacag acaaggctgt ttttggcagg
    37801 aatttctttt ttaaaagagt taaaacgtga gaaaatgatc tttattataa aagagttctt
    37861 aagcttttct ttagagaaaa tttcagttac tatggagaac agggcaaaga aaaatcaatc
    37921 attttctttg tatatcatct taccaatata taggaattgt tagtaacaat tgcctttcaa
    37981 ggaggaatcc atccatgtta ttaggctcac acgtcagcat gagcggaaag aaaatgctgt
    38041 taggagcagc agaagaagca gccagttatg gatcttcgac ttttatgatc tatacaggcg
    38101 cccctcaaaa cactagaaga aaagccatcg aagagatgaa tattgatgcc ggaaaagcgt
    38161 acatggcaga aaaaggtcta acaaatatcg tcgttcatgc cccttatatc attaatttgg
    38221 ggaatacgat caaaccagaa atgtttcctt ttgccgtcca atttctgaga gaagagattg
    38281 ttcgtgcgga agctttaggc gctactcaaa ttacacttca cccaggtgct catgtagggg
    38341 caggaacaga agccggaatt gctagaatta ttgaaggctt gaatgaagtt ttgacaaaag
    38401 aacagacggc acaaatcgct ttggagacaa tggcaggtaa aggtacggaa atcggtcgaa
    38461 catttgagga attagcgcaa atcatcgatg gagtaaaatt gaatgaaaaa ttatccgtta
    38521 cttttgacac ctgtcatacg aatgatgccg gttataatat taaagaagac ttttcaggtg
    38581 taatggaaga gtttgataag atcatcgggt tagatcggct taaagtactc catatcaatg
    38641 attctaaaaa tccgcaaggt tcgcataaag accggcacgc caatattggt tttggaacaa
    38701 tcggattcga cacgctgaat aagatcgtcc atgatccaat gtttgacaat atttcgaaaa
    38761 tcttggaaac tccctatgta ggcaaagata aaaagaaagc ttacgcacca tatggaaaag
    38821 aaatcgccat gttcaaagcg caaacattcg atccagaagt gtttcctgag ttattagcag
    38881 aagaagtgat actctaagat tttctatctg acagcagcaa aaaaggacca ggcacattgc
    38941 cttgtccttt tttgtttaag aaacaaagga gtactttttg cagtcttttt catcatgcat
    39001 cttctccccc ctctttttaa atttagacaa acaaggtata ctggtagaaa agtaaaaaaa
    39061 cgaaaagttt cacaagcaag tggcaaaacg tgattggaag gacgaaaaga tgctgaacac
    39121 tttaaaaccc ttgatcaacc aactaaaaaa gaaacataca tactttattc ttggcatagc
    39181 cctattattt acaattattc gtttgtattt atatagtaaa gcaatctatg gaacagacat
    39241 gtcggcaacc tatgatgacc agctgctgat tcgctatgct caaaatattt tggacggtaa
    39301 atggctaggg gagtatacga ctacaacact ttccaaagga atctcctaca gcttattttt
    39361 agtacttgca cataaactgc atctttctta tgcagttttg ttcggcatgt tcagtatttt
    39421 tgccagtata ttggttgcac tcgcgtttaa accgattgtg aagaacaagt atcttctttt
    39481 aagtatctat ttttattttt tgttttctcc gattagtttt acacatgaat attccactcg
    39541 aacatatcgt aatgccctcg tgattccggc tgttgagatc attatcgctt gtttattagc
    39601 tatttattac cgcaaatacg gttctagcag aaaattgctg ccatggtttt tagggctttc
    39661 tgtcgctttt cctttctttt ggttcattcg cgaagattct atctggcttc ttccttttac
    39721 tgtagtagct ttgggtatca gtttcgtcca gatcttgcta gggcaagact tttctttcaa
    39781 agaagtaaag aagttaaaaa aacgactctt tcttctctcg aaacgggaat ggctgaaact
    39841 ttcattgttt ttacttccta tagttggctt ttccatcgtc tatcaatgga tcgaatataa
    39901 aaatgagaca acatacggta ttgcaaccat caatgaccgt tccagtggag aatttggacg
    39961 tttgatgaaa caactgatcc gaatagatga tgaaacgaac ttgaataaag aaaacagtga
    40021 gatatgggtc tcacatgctg cactaaataa agcattagcc gcatctccta cattccatac
    40081 tgtttctacc cgtattgacg aattatatac gactcacgcg tggactcaag gtggaaaagt
    40141 aaaagaatta cccggagata tcatcttttg ggcattgcga gatgtggtaa gcgaatctgg
    40201 ttattaccaa aacaatgcaa aagaaaccaa tgatttttgg aaaaaagtca accaagaatt
    40261 agaagaagcc tatcacaagg gaacaatcaa aaagaaaaaa gagttctatc tgatgggatc
    40321 aggagatgga aagacgatga gtgatctgcc aatcgtattc gatttccttc aatcaggata
    40381 cgcttatgca tggcgatata aagatttcca tcaaggagga aattatagtt tcggaacgca
    40441 agaacaagtc aaacaggctc aagaattatt aaaccttccg ctaatggaga attggatcga
    40501 tagtaatgaa ccgagaacac ctaatttcca attaacaaaa tcagcaaaag tcgcgaatgt
    40561 catcataaaa atttaccaag ttctggctat tcccgtgctg attgcttcaa tagcagggat
    40621 attacttatg atactaggaa ccgtatttgc aaaagctgat cgtgcctatt atagttcctc
    40681 tttgattgtg gtcaccggat taatactgac acaattggtt tttcttttcg gtgtctcatg
    40741 gttttgttct tatgcgccag aaaaaaagga ctatttttta agtgtctaca ctggtgcagg
    40801 cgtgccatta acgcaattca tcgtagtaac aagcatgcta gttttcagta aattaaatct
    40861 gaaaaaagct ttatcttcta aataaaaaga cccttggtag ctagattttt aacctaacta
    40921 tcaagggtct ttgtgtggaa aaatcagttt ttattcgatt tcttctaccg tttcctgcaa
    40981 aagctgttca tattttttgt ttcctttata taaatcacca atcgtccatg ccaacaggag
    41041 aaggacaaga acaatgatta cataagcgat gtgatcagcc aatgctagtt gagcggaagt
    41101 ggcttgctcg ccgaagaaac tttcgatctg tcctggtaat cctcgtaagt tcaagtaagt
    41161 tagactaatc acagagaacc agccaagaag ttttacgatg aatgaatttt taaatcggct
    41221 acccatctcc gcttggctgt tggtcatcat cagtaaagga atcatcgaaa acggcaacgc
    41281 aaaagccaag aacacctggg attcattcat taagttattc aaagcggcat gttcttcgat
    41341 cgttcctttt gtacttgtaa acaaaacaca aatcaataca ggaatcacag aaagcaatcg
    41401 tgtgaccagt cttcttagcc aaatcgggat acgcatatgg atgaatcctt ccatgatgac
    41461 ttgaccggtc aacgtaccag taatagtaga gttctggcca gaagcaagaa gtgctacagc
    41521 aaataaagtg gacaagatac cagattttgc tacagcaatc aaaataccat tgctcatagc
    41581 tgatggatca gataaagctt catacagacc aaaaaaggaa ggatcttgga cagaaccggt
    41641 tttgaagaca gcgacaccca tgatcaatag cagtgcattg actagaaaag ccattgacaa
    41701 ctggatatta gaatcccaag tagaaaaacg tacagcttgt gctacatctt cttcgttttg
    41761 gtgatcgatc ttccgagttt gtgaaactgc tgaatgaaga tataagttat gcggcatgac
    41821 tgttgcacca ataatcccta aagctccagt cagcggagtt tcgccgccga tgaccggtga
    41881 gtctgagaat gtatgactgt tagggataaa gccagcaaat aaactgtgcc aatctgggtt
    41941 agacaagcca acttgataga caaaaacgaa taaaatgacg aaaattaagc agacaaccag
    42001 tgcttcgatc ttacgaaacc cgacttttgt cagtagtaag agaacgaaaa catctaatac
    42061 agtaataaag acggcaatca ccaatggaat atcaaaaagt aagtagaggg cgattgctgc
    42121 accaatgact tctgcaatat ctgttgccat aatggctaat tcagtcaaga tccataaaat
    42181 gacacctaat gtcttacttg tgcgtgctcg gattgcctgt gccaaatcca tttggctcac
    42241 gattcctaat tttgctgcca tatactgtaa aagcatagca atcagactag acatcaaaat
    42301 aactgacatc aataaatatt ggaaattttg tcctccggtg atcgaagtag accagttacc
    42361 cgggtccata tacccaactg ctactaacgc accaggtcca gagtaagcaa gaagtgtttt
    42421 aaaaaaactt ttcccctttg gtacatcaac tgttccattt acttcagcta aagataaacc
    42481 atttgtatgt tcaataagtc gatgcttatg aaattgttta ttggattgat tattcaagaa
    42541 aaaaccacct ttcctattta tctacgatta cattctacac ctcaaaaaga aaaaattaaa
    42601 gaaaaagatt cggtacgcct aatttttttt catggcgcgt cttgctagat gacgaaagaa
    42661 attttgttct cctctaaaaa aaccactatt ttttgcatga aactaaagaa tacgcctttc
    42721 ttttttcaat aaactttagt aaaataagtc attgagagca attcggaaat agaaaggaga
    42781 gatttatatg ttatcaacag gtattgtcgt acttattgct attattgctt tgctaatcgg
    42841 cgcagtaggt ggctttttcc tagcgcgtaa atatatgaaa gactatttag aaaaaaatcc
    42901 tcccgttaat gaggacatgt tgcgatcaat gatgatgtca atggggcaaa aaccatctga
    42961 gaagaaaatt cgccagatga tgcagcaaat gaagaaccaa gggaaaaaat aggttctgac
    43021 ccaacgactt tttacgggct gattttttca gtccgttttt tatttcgtaa tttgttagac
    43081 tattcagatt attcagcaaa aaagacaagc acagacaatc ctaggctctt gataaagaca
    43141 aaggaggagt aaaatgtcga tttttagaaa attaggctgg ttttttcgtc aggaaaagaa
    43201 acattattta attggggttt tctcgctatt cgcagtggcc ttggtacagt tagttcctcc
    43261 aaaagtgatt gggatcatca ttgatgagat cgccgataaa aatttatcaa taaagatcat
    43321 tttcgggtgg atcttcctat tgattatcgc tgctttttta caatatcttt ttcgttatat
    43381 ctggcggacg aatatttggg gaagtgcagc tcgattggag aaagaacttc ggcagcagtt
    43441 attcaaacat tttacagaaa tggacaatct cttttaccaa aaatatcgga ctggggattt
    43501 gatggctcac gcaacgaatg atttgaatgc gatccagaat gtagcaggag ctggtatctt
    43561 gacctttgca gattcagtga ttacgggcgg aacaacgatc attgcgatga ttttgtttat
    43621 tgattggcgc ttaacactga ttgcgctgat tcctctgcct ttgctggccg tcacttcacg
    43681 agtgctgggg tcaaaactcc atgatgcttt tcgtgattcg caagaagctt tttctagtat
    43741 caatgataag acacaagaaa gtatcactgg gatcaaagtc attaaaacat ttggacaaga
    43801 aaaagaagat ctagcggatt ttgaagaaaa gattgaagat gcaattatta aaaacaaacg
    43861 agtcaatttt ttagactcct tatttgatcc attcattacc ttgattatcg gtctgtctta
    43921 tgttgcaaca attattttag gtggaaagta tgtcttggaa ggcacgatct cgatcgggca
    43981 gatggtctca tttattagtt atatcggtat gcttgtttgg ccgatgtttg cgattggtcg
    44041 attgttcaac gtattggaaa gaggaaatgc ttcttatgat cgcgtcaatg aacttttgca
    44101 tgaaaagacg catatcgtgg aacaactggg tgcagtgcag accccggcaa aaggcgagct
    44161 gaatgtagct atttcctctt ttaattatcc gaaagaagaa tcaccagcat tagaacaaat
    44221 caagtttgcg atccaagaag gagaaacatt aggtattgtt gggaaaactg gtgcgggaaa
    44281 aacgacgatc atcaaattac tgatgcgtga atacgatgat tatcaaggga aaatccaatt
    44341 tggcggacgt tcgatcaaag aatattcatt gaatgcttta ttaggatcaa tcggctatgt
    44401 gcctcaggac cacttcttgt tttcgatgac cattcgagat aatatccgtt tttctaatcc
    44461 aaaactttca gaagaagaag tagagcatgc ggcttcttta gcatttatca acaatgaaat
    44521 caaggaatta ccaaaaggtt atgatacctt agttggagaa cgtggagtct ctttatctgg
    44581 cggacagaaa caaaggattt cgattgctcg agcattgatc aaaaatcctg aattattaat
    44641 gttagatgat gcactttctg cagtagatgc caaaacagaa gaagctatct tgcataattt
    44701 aaaagaaata agaaaagaga aaacaactat cattgcagct caccgtttaa gtagcgtaat
    44761 gcatgcaaaa gaaatcattg ttgtcgatca agggaaaatc attgaacgag gaacccatca
    44821 tgagctgctt gcactaggcg gctggtatga acaaatgtgg gagaaacagc aattagaagc
    44881 gaagatagaa ggaggcgagg cctgatggat gaaaattatc aatcggaatg gacaaaatcg
    44941 attccactta aagaacagaa aaagatcatc acacgactga tgaagttcgc caaacctttt
    45001 agaagaacgt tcatcgttgc tatcttattt gcgtttgctc tgtctgtgat caatgtccta
    45061 ttgccaagga tcatccaaac ttttatggat gatcatctgg ctaaacagtc ggctacgaca
    45121 caagttattt tgttttttgc tggtatgtac ttatttggag tcatagtaaa aagtatcatt
    45181 tggtttttcc aatggtattt gtattccatg gcttcattga agacctatca gtatattagg
    45241 gtaaaattat ttgaaaagct gcatacacta ggtatgcgtt attttgatca gacaccggca
    45301 ggttcaaccg tttctcgagt gacgagtgac acagaaacgc tatttgaatt ttggtacgtc
    45361 tttttgatgg tattgactgg gatctttgca gtcgtttctt cattcattgc catgtttcaa
    45421 atcaatggag aaattgcctt gtggactttg gtgttcttgc caattttagg aatcgtcatc
    45481 tggtattacc agaaatttag ttctcgtatt taccggaata tgcgagaaaa attaagtcag
    45541 ctgaatacga aattgaatga atcgatttcc ggcatgcgta ttatccagca gtttcgccaa
    45601 gaagctcgtt tagctaaaga atttgaacaa gtgaacaatg agtatttaga cacgcgttat
    45661 gcgatgatga aaacgaattc gattctatta agtccgatca tcaattttct atatgcactg
    45721 gcgattgcgt tggtgttgac tttgtttggg atcgatgcgt tgcattctcc catagaagtt
    45781 ggactgatct atgcatttac gacctatgtc cagggatttt ttaatccaat gtctcaaatg
    45841 atggactttt tgagcatctt cacagatggg atggttgctg gtagtcgtat cttgaagatt
    45901 atggatacag aagaatatgc accacaacaa aaccctggag cagatcaaat gattacttca
    45961 ggtaagattg agtttcgcaa tgtaagcttt tcttatgatg gagagcacaa tgtattaaac
    46021 aatatctctt ttgttgctaa tccgggagag accgttgcat tagtgggaca caccggaagt
    46081 gggaaaagct cgatcatcaa tgtgttgatg cggttttacg aattttatga aggtcagata
    46141 ttgattgatg atcatgacat tcgagaatat ccaatggaag agttacgtaa gaaaatgggg
    46201 ctggtcttgc aagatgcttt cttgttttat ggggatatcg caggaaatat tcggatgatg
    46261 aatccagcta tcacagacga acagatacgc catgcggctg agtttgttca agcaaacaaa
    46321 ttcatcgatg aattaccaaa aggatatcac tcaaaagtga tcgaacatgg tgccagctat
    46381 tctagtggac aacgacagct gatcactttt gcgcgaacaa tcgtgactga tcctaaaatt
    46441 ctagtattgg atgaagcaac agcaaatatc gatactgaaa cagaaggatt gatccaagaa
    46501 ggattggcga aaatgcgaca aggacggaca acgattgcga ttgcccatcg gctgtcaacg
    46561 attcgagatg cagaattgat tttagtttta gacaaaggtc atatagtaga acacgggaat
    46621 catgaaacac ttctagcgca aaatggtttg tatgcagata tgtacaaact gcaaagcagt
    46681 ggtatataaa agatgttgtg acagtagcat tttgacctaa gcactagagt gaaaataaaa
    46741 catagcttcc catatttttc atctgtgaga gttagtgtcg tagttaccag atttaaaaaa
    46801 gacgacgcca gctagtcaga agtgcctagt agcccctaag aaagcgttga caaaatcgtt
    46861 tgctttcgat aatacaaaga aaacgataca acttggatag tgattgccta atgcaagcta
    46921 aacctgatga gtctcccatg gtttcatgga cggctttttg ggtttttctt tttgttttgg
    46981 atacagccct atatatcatg gaaaggagca aaacatgctg attgagaaag tactgaataa
    47041 caacgtcgta ataaccaggg atgaaagaaa tcaggaaatg atcgtgatgg gaaaaggttt
    47101 ggcattcaaa aagaaagtgg gtgacccgat ttttgactca tgcatcgaca aaaaatttca
    47161 gttgacaaat gacggacttt cacaacagtt ccaagaattg gcacaagcga ttcctcttga
    47221 gtacttagaa atttcttgtg aaatcattga atatacaaaa gaggatctac aagtccattt
    47281 gaatgattct gtgtatattg cattgacgga tcatttgtat acagctatcg aacgtcaaaa
    47341 aatggggatc tttgtgccaa atgtattgct gtatgatatc aaacaactgt ttcctaaaga
    47401 gtatgccatt gggaaaaaaa cagtgaagaa aatctcggat aaataccaca tcaagttatc
    47461 agaagacaaa gccggattta tcgcattgca tttagttaat gcacaaggca ttcaaagcca
    47521 gcaagacaat acctacctgt ttaccacaac aatacaggat attttaaata tcgtccgctg
    47581 ttactataag attacttttg atgaagattc agtccatttt tttcgatttg tcacacacct
    47641 acgttttttt ctgacacgtg ttctagatgg agcgaaatca gaagatggag aagtttctga
    47701 ggaattgttc caagttattc aaaaaaaata taaggatacg tttggttgcg ttgagaaaat
    47761 agcagacttt ttaaccgggg ctttcgacta ccagatgtcc acggatgaaa aaatgtattt
    47821 aacgatccat actgcgcgtt tagtgcatga gaatcgttgt aaataaataa aaaggaggac
    47881 aaattaacac gaacgtatga aataagggta gtgacattca gttggctaaa ccttccacta
    47941 attaattgga gggaacagaa atgggaaaat accaagaatt agcgagcaaa attgtagaaa
    48001 atgtcggcgg gaaggaaaat atcaatggtt tgacacattg tatcacaagg cttcgtttca
    48061 aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa aaatatggac ggtattgtga
    48121 cggtaatgcg agcgggagga cagtaccaag ttgtcatcgg gaatcatgtg cgagtcgtgt
    48181 ttgaagaggt tataaaagca ggaaacctta cttttgatga ggctgtttca acagaaaaaa
    48241 tgagaccgtt tgatatgctt attgatggaa tatcgggatg tttccagcca ttccttgcta
    48301 tcctggctgc tggtgggatg attcgtggac tgacagcatt acttgtcttc ttaggcgcgt
    48361 ttgatcgcgg ttctggaaca tttgttatgt tcgacaatat cggtgattct gtattccaat
    48421 ttatgcctgt tattatcggt ttaacagttg cccgtaaatt taaagtaaat gaattcgtag
    48481 ggatgttgct tggcgcagca ttaatgaatc ctaacttgtc gctggaagtt ttgagtggtg
    48541 cagcagaagc accactgacg accatatttt ctggtactat ttttgaagca cctatttatc
    48601 aaacagtatt tggcattccc tggattgcgc gtaactacgc ttcttctgtc attcccatta
    48661 tctttattgt gttgttagct agccaaattc agaagccaat taaaaagtta gtacctgaga
    48721 tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat tacaatgccc ttaggttttt
    48781 tactcgttgg accagcattc acattcgcaa ctgatatttt aatggctgga tttgaaacac
    48841 ttttagcact ttcgccagtt aattatggag caatcgtagg cttcttctgg caaattctgg
    48901 tgatgtttgg tctccattgg gcaatcgtac caatgggatt gatgcaattt tctgtgaatg
    48961 gctggcaaaa tatcatgaca cctgttgcag tagtgtcttt tggacaaact gcagcactta
    49021 ctgcactcta ctttaagctt agaaacccaa aagacaaagc aatcgcaatg ccagccattg
    49081 tttctggtat tgtggggatt acagagccag ctatttatgg gtttacattg ccacgtaaaa
    49141 aaatatttat ttttacctgt gtcggtggtg cgttaggggg cgcgtatagt gggttaatga
    49201 atttaacttc ttggaatcaa ggtggactag gcatctttac gattcccaac tatattagac
    49261 cagatggtga tttaacagat gtgattaatg tattaattgg gatcgccatt gcgatggtgg
    49321 tatcctttgc gctgactttt ttcttttgga aggatgaagc aggtgaaaca gatgatatac
    49381 agaaaagatc gggtaaagaa attgtaaaaa caccgataca aggacaaatc gctccactaa
    49441 atgcagcgaa ggatgcagct tttgctcaag gaacattagg tcgagggatc ctgatctacc
    49501 ctgaaaaagg agaagtacgt gccccttttg acggaacagt tatgaccctg ttcccgacga
    49561 aacatgcaat cggaatggtt tctgaaacag ggctggaatt gctgattcat gtcggattgg
    49621 atactgtgca gttggaaggg aaatattttg aaagcttagt gcagcaagga gaaagagtga
    49681 aacaaggcga cctgttgcta cgcttcgaca aaaagaaaat cgaaaaggaa ggatacagtt
    49741 tagaaacacc tgtgatcgtc accaattata cggattattt ggatattttg gaaaaccaag
    49801 gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg gttaggtgaa
    49861 gaatggacta ggcttaacta ttctttttat tccacttctt tattttcaaa attgaaaggc
    49921 tctttcatga tggctgagct gatagggcgt ttatcgaatt tttgaccgac agaaattaga
    49981 tggactaaat tctgctgata attttctgca aaaacacaac tatataacac gtctagaaga
    50041 taacaaattg atgtattgat cgtaaaatta ccaatttttg aatacagtcg ctcgcgtgtc
    50101 gtaagtcgca aagcggctgt actcattgca gatagcgttg aatcaccgat actggtcaaa
    50161 gctaaaattg gaacatcatt ttgtttcaat atttctgcgg tacgaaggat cacattgttt
    50221 tcacctgtat aagaaatcag aatcccgcac atatcgctcg tgcaattgta cgcttcatag
    50281 aatgattcac caaaagtggt gacaattgtg acatccattt tgatgcgctt cattttcagc
    50341 atgaaatctt gagaaatcaa agtattcgag ttactggcga agatcacgat tttttttgcc
    50401 tggagcagta aatacttagc tttttgcagt tggtcatgat gaatgagaga aagtgtatct
    50461 tgaatcgtca tctgttctaa agaagccatt ttatttgcga tcgtcataat accgtctgtg
    50521 gtggaaaaag gaagattcgc atctacgtct tgaaaattcc cttcaagata ggctgtttca
    50581 gctaaaaaag cattacgaaa actgacccag ccatcaaacc ccattttttt tgctacacgg
    50641 atgagtgtgg acgggtggac ataacatcgc tcagcgatct cttggatggt catttcgcca
    50701 agtgctggag aatcgcttaa gagaaactgg atcacttcat tttctgaagg agaaaaggag
    50761 atttctttga ttttttcacg taataacatg tagatacctt cttcttattt ttttacgtaa
    50821 attataactt ttttgaaaat gatttgtaaa cggatttata ctatataact taaaatagac
    50881 attttttgac aagatattta caaaaatcca ctagaaaaca gtaacttttg taaatgttac
    50941 tggaaagaaa cggatttcta ttgtttgttt tgtttacaaa aaagataatt aaagtgtaaa
    51001 aaagtaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg gtggaggcag
    51061 cagttacacc cccgagttga tggaaggatt tatcaaaaga tatgaggaat taccgatacg
    51121 agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgatatcg ttagtcagat
    51181 gtctcagcgt atgtgggatg cttctccgta tgaagtcaag atctatgcaa ctctggatcg
    51241 ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg gtcaattaga
    51301 tgcacgaatc aaagacgagc ggatccctag ttattacggg atgatcggac aagaaacaaa
    51361 tggtgctggc gggatattca aagcttttcg taccgtgcca gtggtcttgg caatcgttga
    51421 agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc caagtgggat
    51481 ggtgacagaa gcagttatca gatatggtaa atgggataaa gtgattggat tatgcaatgt
    51541 gccagtaatg gcgatgatga cagaaccagc aatgcttggc aagaaaaaag aagagctgat
    51601 ctacaagttt gccggtctaa atcatttcca ttggcacaaa gtagcagata ataaaggaaa
    51661 tgaccgcacg gcagaattga ttgatcgttt atacgatgaa gacaatggat tgccgaaaaa
    51721 tatctttgat atcccgtttt tcaaagaaca attaaaacaa atgaacatga tcccttgtgg
    51781 ctaccaccgc tattactatc gtgaagaaga aatgctggaa catgctttag aagaatatca
    51841 aacgattggt acaagagcgc agcaagtgaa agaaacagaa gcagagctgt ttgaattata
    51901 caaagatcca gaattagacc ataaacctga acaattgcaa aaacgaggtg gcgcttatta
    51961 ttcagacgct gcctgtgaaa cgattacttc aatctatgga aataaagaaa cacagatcgt
    52021 tgtatctaca aagaatgatg gagctgttcc tgacctgtcg gcagattgtg ttgtagaagt
    52081 cactgcctat gttggcgcac aaggagcgag aaatgtagcg tttggttctc ttcctgctgc
    52141 tgaaagaggc tggttgcagg taatgaagaa tatggaattg ctgacgatcg aagcagctgt
    52201 gactggtgat tatgggacag ccttacaggc gtttacgatc aatccactta tacgatcagg
    52261 aaagacagca aaacggatca tggatgagtt gtttattgca caccagcagt atttaccgca
    52321 attcaaggaa acaatcggcc gattaaaaga agaaggaatc gaagttcaag atgaactggc
    52381 aagagaatta tcaagtaata cacatataat ggaggaagta taatgggatt cagaaaagat
    52441 tttttatggg gcggtgctac agcagccaat caatgtgagg gcggttatga tgaaggtgga
    52501 cgcggactag caaacgtaga tgttgtgcca atcggaaaag accgttttcc tgttatcacc
    52561 ggaaagaaaa aaatgtttga atttgatgag gaccattttt atccagcaaa aattgcaatc
    52621 gatatgtatc atcgttataa agaagatatt gctttgtttg cagaaatggg gttcaagact
    52681 tatcgaatgt ccatcgcctg gagtcgtatt tttccaatgg gggatgagaa agagccgaat
    52741 gaagctggct tgaaatttta tgaggatgtc tttaaagaat gccaaaagca cggaattgag
    52801 ccacttgtga cgattacgca ttttgattgt ccaatgcatt tgatcaaaga atatggtgcg
    52861 tggcgttcca gagaactcgt tggtttttat gaaaatctgt gtcgagtcat cttcaatcgc
    52921 tataaaggac ttgtcaaata ttggctgaca ttcaatgaga tcaatatgat ccttcatgca
    52981 ccattcatgg gcgcagggct gtattttgaa aaaggagaaa atgaagaaca aatcaaatat
    53041 caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc tcatgaagta
    53101 gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc atacacttgc
    53161 aatcctgaag atgtttggga ttctagaaaa tcagatcgag aaaattattt cttcattgat
    53221 gttcagtcac gaggagaata cccagcttat gcattgaaag aaatggaacg aaatggcatt
    53281 cagatcgaaa tggaaccagg ggatgccgca ttgctgaaag aacatactgt tgattttatc
    53341 tccttttctt attattcttc ccgagtttcg actaccgaca acgaactctt ggaacagact
    53401 gcaggaaata tctttgcttc tgtcaaaaat ccatatctgc aagcaagtga atgggggtgg
    53461 caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg ttatcaaaaa
    53521 ccattattta ttgtagaaaa cggcttaggt gcagtcgata cgccagatga aaatggttat
    53581 gtggaagatg attaccgaat cgattacctg gcagcgcata tccaggcgat gaaggatgca
    53641 gtagaactag acggagtcga tctattaggc tatacgactt ggggatgtat tgatttagta
    53701 tcagctggta ccggtgagat gaagaaaagg tacggattca tttatgtcga tcgtgataac
    53761 caaggaaatg gcacattagc ccgttcaaag aaaaaatcat tcaactggta taaaaaagta
    53821 attgattcaa atggagaaaa tctttcgaac gattgatcaa tgatttataa aagtctattg
    53881 tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc agaacaatag
    53941 actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga gaatctacat
    54001 atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc tttatatgct
    54061 tcacgttcaa acttatccgt attgatgatc gaacaaggtg cccctggcgg ccaaatgaac
    54121 aatacggcag aaatcgaaaa ttatcctgga ttcgattcga tcatgggccc cgatctagct
    54181 tataagatgt atgaaagtgt ctcaagattt gggacagaaa atgcttatgg aatcgttcaa
    54241 gatatcaagg atcacggcag ccataaagaa gtcatctgtg aagataaaag ttacgaagga
    54301 aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc tggcgaggaa
    54361 gaatttgctg gacgaggcgt ttcttactgt gcagtctgcg atggtgcgtt tttccgtaac
    54421 aaacgtctag tagtcatcgg gggtggggac tcagcagtag aagaagccat ctatttgaca
    54481 caatttgctt ctgaagtagt gatcgtccac cgtagagatg aattacgtgc acaaaagatc
    54541 atccaagacc gtgcatttgc aaatgaaaaa atttccttta tgtgggatac agtcgttgac
    54601 gaaatcgttg gaaacgaaat ggtagtgaca ggtgtgaaag cacgtaatgt caaaaccggc
    54661 aatgaatttg atttagcagc agacggagta ttcatctatg ttggactgga tccattaact
    54721 gcacctttta gagaagcagg tttgacgaac gaagctggct ggattccaac agacaacgaa
    54781 atgaaaacga atatgccagg cgtttttgcc atcggagatg ttcgtgaaaa agatttgcga
    54841 caaatcacta cagctgttgg tgacggcggt attgctggac aacaagtgta taaatacttg
    54901 gagtctttag aagaaacaac agcataagtt taaaaagagt gaaagaacgg ttttaattgt
    54961 tcttttactc tttttttttc gcgtgaaatc cactgaatcc gatttatgtg aatacttgca
    55021 atcatgcttg ataaaagaat cctttcttga tttaagcgtt ttttggtcct catactttca
    55081 tatctaataa gtgaatgcta tacttattaa gaaccatatt tcagaaagag gtgttatttt
    55141 catgtcttgg gaacaagtgt atgaacaatg ggtacatgaa gaaaatttag aagaaaactt
    55201 aaaaaaacaa ttaaatgatc tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc
    55261 accgttggaa ttcggtactg caggcatgcg gggaatctta ggacctggga tcaatcgaat
    55321 gaacatctat acggtaagac aagcaacaga aggactagca cgtttcatgg atacacaagg
    55381 tgaagaaaca aaacgccgag gtgtcgctat cgcttatgac tcacgccatc agtcaccaga
    55441 atttgcaatg gaagcagcca aaacactggc acatcacaat atcccgtctt atgtattcga
    55501 aagtctgcgt ccaacaccag agctttcttt tgcagttcgt catttgaaga cgtttgcggg
    55561 aatcatggta accgcatctc acaacccagc ggcttacaat ggatataaag tctacggaga
    55621 agatggcgga caaatgcctc ctgcagacgc agatgcattg acgaaatatg tccgtgaagt
    55681 cagcaatccg ctaaaagtcg aagtattatc tgacgaagaa gctaaacata gcgggttgat
    55741 cacgatcatc ggagaagaag tggacgctgc ttatctagaa gaaatcaaag cagtgacgat
    55801 tgatcgtgaa ttggttgaaa caatggggaa agatttgaaa cttgtgtaca cgccacttca
    55861 tggaacaggg aaaatgttgg gcgaacgcgc gttgaaacaa gctggattcg aacaattcgt
    55921 actagttccc gaacaagctg tggcagatcc agatttcact acggtcaaat cgccaaatcc
    55981 agaagaacat tcagcttttg aatatgctat acgacttgga gaaaaagaag gagctgactt
    56041 gctgattgca acagatcctg atgcagaccg tttaggcgca gcggttcgtt tgccagatgg
    56101 ttcttatgaa gtattgactg ggaaccaaat cggagcattg tttgctcaat atattttgga
    56161 agcacatcaa tcaagaggca ctttaccaga aaacgcagcg gtattgaaat caatcgtttc
    56221 cagcgaattg cctacagcaa ttgcggaaca ctacggtgct accatgttca atgtgttgac
    56281 agggtttaaa ttcattgctg aaaaaatcca acaattcgaa gaaaaccatt cacatacttt
    56341 tatgtttgga ttcgaagaaa gttatggtta tttagttaaa ccttttgttc gcgataaaga
    56401 cgcgatccaa gcattgcttt tattagcaga agtagcagct tactataaaa aacaaggcaa
    56461 aacgttgtat gacggcttgc aaacgatttt tgaaacctat ggctatttcg cagaaaaaac
    56521 gatttctgtg acaatgagcg gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa
    56581 attccgtaaa gaagcaccga cagaatttgc cggagcaaaa gttattcaaa cagaagactt
    56641 taaagaattg acgagaacag accgtgaagg aaacgtagaa aaactcacaa caccgccatc
    56701 cgacgtattg aaatatacat tggaagaaaa tggctggatt gctatccgcc cttcaggaac
    56761 tgagccgaaa atcaagtttt atattggtgt aaaaggaagc tctacagaag acgcagccaa
    56821 aaaagtagcc tcttatgaag cgattatcaa aaagatgaca gaagaataaa aaacttgaga
    56881 ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt tcaagttgga ggtgaatgga
    56941 ttgccagaca cgcaaaaatc aaaagtagag aaagtcagtc aattattcaa aatgttgagt
    57001 gatcccactc gtcttaaaat tctgctatat ctgaaagacg gggaacaaaa tgtgacagca
    57061 atcacccaag cagtggaaat ggagcagtct gctgtatcgc atcaactacg tttattaaga
    57121 gaaaatcatg tcgtcaagtc tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat
    57181 tctcatgtac tggatatttt gaaccagaca ctcaaacatg tggaacatca ataacgatag
    57241 gtgcaaatag ataaacactg gagtgtgaca acgaaatttc ttcgttgtca cactccagtg
    57301 ttttttaaga aaaagacaaa aatgatcaat cgcttttttc tgttgtttca gttccataat
    57361 aactatgatc ttctaggtct aaagcattca aaataagcga agccgtttct tcaatagata
    57421 aagatgctac attgatcact acacatccta gtttttcata aagatcattc gcaaaatcga
    57481 gttcagctct aattttttcg atatcggaat aagcagtatc agggtttaat ccgtaagagc
    57541 gcatccgttc tttacggata ttatttaaaa tatctgggtt attcgttagt cccacgattt
    57601 ttttaggatc gatctcccaa agctgtttgg gaatatgtgc ttgtgggatc aaaggtaaat
    57661 tggcaacttt taaatttttg ttagctaaaa acaagctcaa aggcgttttg gaagttcggg
    57721 atacgcctag caaaacgaca tctgcttcta aaaaaccacg aggatctttc ccatcatcgt
    57781 attttacagc aaattccatt gctttgatcc gtttgaaata gttctcattc aataaatgga
    57841 gggcaccagg ttgccgaatt ggcttgatac cagttaaacg ttcaatttct gatatcggtg
    57901 gcgtcatcac atcaaaacta aataattgat gttcttcaca aaaagaacgt gttatctcga
    57961 tcagttcttg attgattaat gtatgcagga caagtgcttt ttcttctaat gcatcttgca
    58021 atgcctgcct taatgttttt tcatctgtca cgaacgttct gcggaatagg ttgaactcaa
    58081 cggtaggata ctgagccata gtagcttgcg ctagttttgt cgcagtttct ccagcagagt
    58141 cggagatgac aaaaaaagta acgatttctg tagaatcgga agtcatggat aaaacctcac
    58201 ttgcattttt tttatgttta ttataccata ctctagaatg cgtacttcgt aatcattttg
    58261 atttggagga ataagtcatg aaaaaacaga cattgatcga acaatcatta gaaacattga
    58321 aaaataacgg ttttaaatat actaaaaaaa gagaagcatt gttaacttat ttagtgaagc
    58381 gtaaccgcta cgtatctgct aaagaagttt ttgactatat gaatcaggaa ttcaaagggg
    58441 taagttatga tacgatttac cggaatctcc atgattttgc agagatcgat ttactggaag
    58501 aaaccgattt aaatggagaa atgaagtttc gttttcattg ctgtcagggg gaaatcggcc
    58561 atcaccatca ttttatctgt acagtgtgcg gaacaacgaa agaaatccat atgtgtccga
    58621 tgaattattt cgaagaacag ctaggtggat gcacgatcga agggcataga ttcgagattt
    58681 tcggaagatg tgaaaattgt caataaggat ttaaataaaa taacatttga taatttaaaa
    58741 aaaaagcaag atttccttga cggtcaaaac agcattagat ataatattta caggacagtc
    58801 ttgttttttg agatatattt caataacaat tacggttact ttttaagctc ggagggaggg
    58861 aattaacatg tcaaaaacag tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg
    58921 cttcaaacgt tccgtgtcaa aagcgggtac tttacaagaa tctcgtaaac gtgaattcta
    58981 cgaaaaacca agtgtaagac gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt
    59041 ctagttctgt gatgaattgg agttggtagt gtgtcactac taagtagatt aaacgatgac
    59101 atgaaagcag cgatgaaagc gaaagataag gaatctttac aagtcatccg aatgatcaag
    59161 tcatctatac aaaacgagca gattaaagaa ggtcacgact tgacagaaga agaagaacta
    59221 acggtgttgt ctcgtgaaat gaaacagcga cgtgattctt tgcatgagtt tgaagaagca
    59281 ggacgtgacg atttagctga aaaagtaaaa agtgaaattg taatagtcga aaaatatatg
    59341 ccggaacaac tttccgacga agaaattcga caacttgttc aagaagcaat cactcaaact
    59401 ggtgcttcat caatgaaaga atttggcaaa gttatgggtg caattatgcc taaagtcaaa
    59461 ggaaaagcag atggaaacca agtgaatgcg attgtgaaag aactattaca atcttaattt
    59521 tatcatttaa actgcggaca gtgattatcc gcagtttttt gtgttcgtaa aaaaagaact
    59581 aaaaaacagc tagaagaaag aatcttattc tttcttctag ctgttttttt cctgtttaat
    59641 atctcttatg ttaaattgtg tttgatttgg ttattcttct ggtaactcac ttgcttcttg
    59701 tttatatcct gatacgcgat atacataata aaagagcaag ctgccagcgt aaccaagcaa
    59761 cgttgggacg acccagccaa gacctaatgc agcaaaagga acataatgat gataaaatcc
    59821 tagtaagctt gctacgacag tttgctgact cactaatgca ggcgaatttg ccaacatatc
    59881 caatacggct ggaacggcag aaaagaacat cgttacttga tagacaaatg gactcttttg
    59941 gaagaatttt gctgttaagg aaagtaagat taacgcaagt gacaatggat acaaaagcat
    60001 caatactggt aaagaccatt ggatgatatt atccaatccg gcattcgcaa cgacaaaaga
    60061 gacaaaagtc gttaaccgca gccatgtcat ataactgact ttagggaaca gtttatggaa
    60121 atcttgtgca aaagaaacaa cgagtcccat agcagtagtg aagacaccta atgtgaccat
    60181 tacccctaag aagctagcac cgaagttacc taaataaaaa ttagtgatct gagaaagtgc
    60241 agttccgcca ttttctgata aagacatctt attcaaactc atcgcaccga gaaggaccag
    60301 accaaaataa atcatgactt ctaacgcaac gctcaggcta ccagcttttg ctgttaattc
    60361 tgtgactttt ttatctttaa aaccaagatc acgaactgca tggacaaaag tgacacttaa
    60421 agcaagtaaa gctactgcat caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc
    60481 gttcgtctga taagaatctg cagcagattg gtttagattc cccatgggtt ggaataaaga
    60541 aacgacaaaa atgatagcta ataatgcaag aaacaaggag tttaaatact ttccgacata
    60601 tttgaccaaa cgtgttggat gtacagtaag gaaataagca agtaaaaaga aaagagcaga
    60661 gaataaaaac atacctaatg aatcatattt ttcaggcaaa aagggtttag cagccatttc
    60721 aaaagcagtt gctgctgtcc gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag
    60781 gatcaagaac aaggcagcat accattttcc gacaggttta gctaagtcat acagaccatc
    60841 actttttgtc actactacag ctaagatagc taataatggg aaaagagcgc ctgagatggc
    60901 aaatcctaca ccagcgctga tccagtttga tccagccatt tgacctaagt gtacaggaaa
    60961 gatcagatta cctgacccga agaacatccc gaagataagt gaactgacca tcaataattg
    61021 cttccatgaa taagtttttg tttcttccat aaatttttcc tccaataatt atcattttga
    61081 gtaacaaaat aaacgttgca gaaactagaa ataccactaa tatcagattc catgaaaacc
    61141 accaccttaa aatataaaaa aagccctttt ccacttggga aaagggctct agattctaga
    61201 acggtaccac cttttttcgc ttaaaaaagc ttcttcattc acggacgctt ttcgataaag
    61261 ttatccgcta gcctgataat ggaggcagcc acaatgatct acgttttcga cattgactcg
    61321 gaaatgatgt tcgtctattc gatttattgt catttctcac ttacatgact cactgggaat
    61381 aaatcactga atagcctact gttttcctcg acgctttgct gtatatttat ttgttgacgt
    61441 tataatacac ctgttttttt tgaaatgcaa gaatatttca aatattttgt tttctttttt
    61501 tgaaacaaga aaaataccca gagaatactt gctaagttag acggaatact tgtcctctat
    61561 cttatgcata gcttacatga tcagcttttt ttattcaaac gagaccttta gagaatggaa
    61621 gcggctagtt ttttaacgtt tcttggtact tatggttaag caactctttt agaacctttt
    61681 cttatttgct atatagtttc ttctgagtat ggtaaaatag gaaagaatta atcgaaaaaa
    61741 aggggatgtc tatttttgac ggaagagtct agttctttag agattagatt gacggctgct
    61801 gatgatgtca gcatgctttt aggtgcacac gacaagcata taaaactgat tgaggaaaca
    61861 acggaaacaa ccatccatac acgtggagaa atcattcaga tcattggtga gcaatcccag
    61921 atttccttgg ctgcctcagt cattcggaca ttacaggaat tgatcaaacg aggaatccac
    61981 gtaagtacgc ccgatgttgt gacggcattg aaaatggggc aaaaaggtac attagattat
    62041 tttgttgaga tgtatgagga agagatcgtc aaagaccgta atggaaaacc tatacgggta
    62101 aaaaacagcg gccaaaagaa atatgtcgaa tcggtagcca aacatgatat cgtatttggc
    62161 gtaggaccag ccgggacagg aaaaactttt cttgcagtag tattagcaat cgctgccttg
    62221 aaaaaaggtg aagtgcaaaa aatcattttg acgcggcctg ctgtggaagc aggtgaaaat
    62281 ctaggctttc ttcccgggga tctaaaagaa aaagtggatc cttatctgcg gcctgtttat
    62341 gatgctttat accaaatttt tggattggat catacgaacc gattgatgga acgaggcgta
    62401 atcgaaatcg cacctttagc ttacatgcgg gggcgaacat tggatgatgc attcgttatc
    62461 ttggacgaag cacaaaacac gacagtcgct cagatgaaaa tgttcttgac ccgtctcggt
    62521 tatcaatcaa aaatgattgt caatggggat acaagtcaga tagacttgcc tcgagggacg
    62581 acaagtggtc ttgttcatgc agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt
    62641 aattttgaag caagtgacgt tgttcggcac cctgtagttg ccgaaatcat caaagcttat
    62701 gaaaaggcag atctccatca agaataagct ttgcttagga ggataagcac atgttgaata
    62761 aaacactgca gaaactacaa gaaaaacttg gaaaggcagc attgcctttc ctgctgttct
    62821 tgttttttat cattatctgc ggcgttacat ttagtagtgt caaacaaaag gtcaatgatt
    62881 tcagagaagg ccaagtagca gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg
    62941 aagaaaccga acaaaaaaga aaactggcag cagaagcagt cactccagaa tacacttacc
    63001 aaaaagatct tgcagatgac cagaacaatc ggatcaagca actatttgaa ttgatcgata
    63061 agacgaatga tgccatcaac aaatcttatg acgaaaagat tgataaggca aaggaagatg
    63121 aaaatatccc aaagcctact acagaagagc ggatcgccgc gctgaagaaa aattttgaaa
    63181 atgtgaatgc agaaaatgtt gtgttttatc aaaaactacc gaacaatttt tattcgacga
    63241 tatttgaaat gactgaaact gagattcata ctgtgaggga tgagagtctg aagctcattg
    63301 acgaacagat gagcaaacaa gttcgtgaaa gtgaattgga agcttttaaa caagaagcag
    63361 aagagcaaat ccaatatttg aatgtcacac cagcacaaca acaaatgatt cgctacttag
    63421 tggatcaagg tatcgtagtg aatgacgtat tgaatgagaa aaagacagaa gaattgaaac
    63481 aatccgcacg agaagctgta cagccagtga tgatcttcca aggagaaatc attgttcgtg
    63541 aaggtaatca aatcgatgct tcagctatga aaaaattgga attactagga ctgactaatc
    63601 aaacaacttc gatttttccg ctaattgcca tggttttagc tgttttgctt caaatcggcg
    63661 tactgattta taattcactt caattccaaa atgcaggaga aagaacaaaa tacgtgcttt
    63721 tctatgtgac agctatgtca gtcagcatag tgctgatgaa atttttccaa ttattccaaa
    63781 cggaacaagc tacttatatc ccgctgtttt atcccgctgc ttttgcacca cttgttttaa
    63841 gctttttctt gaatcgaaga gcagggatca tggctggtat tttccaagca gtatccgcac
    63901 ttttcatttt ttatgattcg attggcacga actttctaac gattatcttg atgtcttatg
    63961 ttttctctgg gctcatggca acgattgtca gacgcaaacg gatctcagaa caaggattat
    64021 ctgctgccat gtgggtcatt atttttcctg tgatgatgga tgtcattttg atcgtctatc
    64081 aaggaatgag ctttggtgat gcaacgactt ggttaatgct cgtctgcggg ttcactggtg
    64141 cactcttctc attcttgctg acgatcggtc tacacccata tattgaattg atggtcaatg
    64201 atgacagtgt aattgtatta aatgaactca gcaatccaaa tcatccttta ttgaaacagt
    64261 tgctggaaga agcgccaggt acttatcatc atagtatgat ggtagctaat ctaagtgcta
    64321 acgctgtagc agaaatcggc ggacgttctt tgctaactcg agtagcttgc tattatcatg
    64381 atatcggcaa gatcaaacat gctagctttt tcgttgaaaa cttgccttca ggagcggaaa
    64441 acccgcacaa tttcttattg ccagaagaca gtaaacatat tatctttgga catgtgacag
    64501 atggagccaa aatattggaa gattataata tgccgcaaat ggtgatcgat atttgcaaac
    64561 agcatcatgg aaccacgcta atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg
    64621 aagtaacaga agagcaattc cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga
    64681 tcgtcaatat tgctgacagt tgtgaagctg cagtacgggc aatggatcat ccgacgagcg
    64741 aaaaaatcga aagttttgtc cataatctta tcgaagagcg tatttccgat ggtcaattag
    64801 acgatagtgg actgaccttg aaagaaatcc gaaaagtgga aaaatcatta atcagcggtt
    64861 taagtagcac cttccattct cggatcaaat atcctaaaat gaaaacagaa gttgagaaga
    64921 taaaggaaga acaagaagaa tagaaagaag cgatgtataa tggatattac ctttatggat
    64981 gaaacaaacc aagtctcaga agagaaaatg aaagaaatcg atgacttgct gcaatttgca
    65041 gcatcttatt taaagctgcc agaggaaaca gaaatgtctg ttactttcat ggacaatgcg
    65101 gcaatccaag taatcaatcg tgattaccgt ggaaaagatg caccgacaga cgtcatcagt
    65161 tttgctctgg aagaagaagg cgaagacgaa atcccagtga tttttgaaga tggagagaat
    65221 ccattacctc gtgtactagg agacatcatg atctctattg aacgtgctgc tgaacaagca
    65281 gaagcttacg gccattcata tgatcgggaa cttggctttt tagcggtaca tggattcttg
    65341 catatcaacg ggtatgacca tatgacacca gaagatgaga aagaaatgtt tggtttacag
    65401 aaagagatct tggatgccta tggactcaaa agataaaaac gtagagaaaa acaaacattt
    65461 cctgatgtct gtggaatttg ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa
    65521 tatgaggaaa catgttgcgt tcggtattct tgctttattt atgggcgtga ttttccagct
    65581 tgatcggata gagtggttat ggctgctttt agctgttttt ctcgtttgga tagtagagat
    65641 catcaatact gtttttgaaa atgtagtgga catgtttacg gattttcatt ttcatccgat
    65701 tgggaaaaaa atcaaagata tggcagctgg agcagtttta gtgaccgctt gttttgctgt
    65761 catcattggg ttgattttat ttgtccctaa aatatatcat ttgttgttta attaaaaagg
    65821 agaacgtata tgccagaaca taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta
    65881 ggaaaatcca cattattgaa ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag
    65941 gcacagacaa ctagaaataa aatccaagga gtatatacta caccagaagc tcagctgatt
    66001 tttatcgata caccaggaat ccataaacca aagcatcgtc taggagattt tatggtagaa
    66061 gcagcttaca gtgcgttgaa agaagtggat gcagtcttat ttatgatcag tgctgatcaa
    66121 aaacgcggcc ggggagacga ttttattatt gaacgtctaa aaaacgtgca ttctcctgtt
    66181 tatctgatta tcaataaaat cgataagatc catccagacg agttgctggg aatcatcgaa
    66241 gattattcga cgcagatgcc ttttgcacaa gtgattccga tctctgctac agaaggaaac
    66301 aatgtcgaac gcttaatgga tgtattagtc agtgaaatgc cagagggccc acaatatttt
    66361 ccagatgatc aagtgactga tcatcctgaa tacttcatcg tttctgaatt gatccgtgag
    66421 aaagtcttgt ttttaacacg agacgaagtg cctcattctg tcgcagtcgt tgttgattcg
    66481 atgaagcgaa acgagaatga taaaatccat atccaggcca cgatcatcgt cgaaagagat
    66541 agccaaaaag gaatcattat cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa
    66601 gcaagaaagg atatcgaaaa tcttttagga gacaaagtat ttctagaact ttgggtcaaa
    66661 gtccaaaaag actggcgaga caaaaaagtc tatctgcaag acttcggcta ccgaaaagat
    66721 gaatactgat attctgaact tcttataggt taagtataga aataaactgg ataaaaatcc
    66781 gcacgttgag aaaaacggcg gaagatcgaa acataaataa tctggatcgg cagaatctgc
    66841 ttttccagat ttatttttta ggagggagaa tatgagccag cttgcagaat cgaaaggctt
    66901 gattttatct agccgtaatt acaaagaaaa agataaatta gtcaaaatct ttacggaatc
    66961 ttctggcaaa atgatgtttt atatcaaagg aatccatcgg caaaatcagc cattagctcc
    67021 ggctttgctc ccctttaccg aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc
    67081 gtttttaagc agtgtcaaag acgtccatgc gtttcggaac atccaagaag atatcttttt
    67141 gagcggttat ggtacctatt tgctaaatct agtagatgca gcaatcgaag atcatcaata
    67201 tgacccgcat ttattccaat ttacgcaaca agctctgcag cggatggatc aaggagatga
    67261 tgcagaaatc atcacgaata tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc
    67321 aatttggact cactgtgtcg tatgcggaga aacaaaagga aaatttgact attcttccaa
    67381 atatggcggt gtgatatgcg agaaacactg gccaatggac gaacaccgtt atcatgcaga
    67441 tcctcgtgct gtttatttcg tccgaatgtt ttcggctatt tcttatgata agatcagtgg
    67501 aatccacttg aaagaagaaa cgaagcaggc aatccgtgac ctcattgacc agttgtatga
    67561 tgaatatgtc ggtattcatc taaaaagcaa aaagttcatt gatcaaatga agtcttgggc
    67621 acacgtatta aagacgccag agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga
    67681 ataaatgaaa gatacaacaa aaaaatcaaa actatttgac aaatacaggg acttccgtct
    67741 attattaacc gtagattaaa tagatcgttc actgagagga tctattatat aaacaagaaa
    67801 actgcaaaat gaatacgtac attgaccaag atcagtaaaa aacacgtggt ttagcgagtt
    67861 cgggagagtg gaagccggac ccaaacgatt tttgaagagg cacttgcgag tacgataaat
    67921 gaagctgccg aatctttatt cggaagtagg gtggaaccgc gaaattattc gtccctatgt
    67981 ctgtaatcag acatagggac tttttgtcgt tttctttcct gtattcttat tgacagcaac
    68041 gcttaaaaat tgaaaggggc aaaacagatg agtaaaaaat tgaccgtcca agatatgatt
    68101 ttaacattac aaaaattttg gtcatcaaac ggctgcatgc tgatgcaagc ttatgataca
    68161 gaaaaaggag caggtacgat gagcccatat acattcttac gggcaatcgg accggaacca
    68221 tggaatgctg cttatgtaga gccatcccgc cgaccagctg acgggcgtta tggagaaaac
    68281 ccaaaccgtt tgtatcagca tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat
    68341 atccaagagc tttatttaga aagcttgcgt cttttaggaa ttgatccatt agaacacgat
    68401 attcgttttg ttgaagacaa ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg
    68461 gaagtatggc ttgatgggat ggagatcaca caattcacgt acttccagca agtcggagga
    68521 ctaccttgta aaccagttac ttctgagatc acttatggac tagaacgtct tgcttcatat
    68581 attcaagaag ttgaaagtgt ctatgatctg gaatgggctg atggcgtgaa atatggcgag
    68641 atcttcaaac aaccagaata tgaacattcc aaatattctt ttgaagtaag cgaccaagac
    68701 ttgctactaa gtaattttga ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat
    68761 cttgttcatc cagcgtatga ttatattttg aaatgcagcc atacattcaa cttattagat
    68821 gcccgaggtg cagtatccgt aacagaacga gcaggttatc tttcccgtat ccgcaacatg
    68881 gctcgtgctg tagccaaaat ctttgtggca gaacgcgaaa aattaggatt ccctttattg
    68941 aataaaaaca cagcaaatcc agaaaacatg aaggaggctg aataagatgg caaaagactt
    69001 attacttgaa atcgggctgg aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca
    69061 attagaagaa aaaacagcaa aatttttaga tgaaaaccaa ttgacttatg atacgatcga
    69121 aacattttcc acaccgcgcc gtttagcaat caaagtaaca aatattccag aacgacaagc
    69181 ggacagcgaa gaagaagtca aaggaccagc caaaaaaatt gctttggacg cagatggaaa
    69241 ctggacgaaa gcagctgaag gatttgtccg aggacaaggt ctgacgacag atgctatcac
    69301 ttttagagaa ttaaaaggcg tggaatatgt ctatgtaacg aaatacactc acggcaaaga
    69361 agcaaaagaa gtattgacag gtttagcaga tgtcattaaa agcctcactt tcccagtaac
    69421 gatgcattgg gcagaatacg attttgaata tatccgccct attcactgga tcgtggcatt
    69481 gttagatgac gaagtaatcc cattcgaaat cttggatgtc aaaacaggca aagcctctcg
    69541 aggtcatcgt ttcttaggcg aagatgtaac atttacgcat gcaaaagaat acgaagaaaa
    69601 attaaatgaa caatttgtta ttgcttcacc gcaaaaaaga aaagaaatga tcatcacaca
    69661 agcaaacgaa ttagcagaaa aaaatcactg gatcattgat ctagatgaag atttactaga
    69721 agaagtcaac aatctagtcg aatatccaac tgtcttttct ggcgacttcg aagaaaaata
    69781 tctatctgtt cctgaagaag tactagtcac ttctatgaaa gaacatcaac gttatttcga
    69841 agtacggaac gatcaaggta tgttgcttcc tcattttatt tctgctcgta atggcgatcg
    69901 ggtaaaattg gaaaatgtgg tacgaggcaa tcaaaaagta ttgactgctc gtttagaaga
    69961 tgcggaattc ttttataatg aagacaagaa agcttcaatc gaccaatacg ttgaaaaatt
    70021 aaaaactgtt actttccatg aaaaaatcgg tagcatctat gagaaaatgc aacgggtaca
    70081 agtcatcgct cgcatcatag gcgaacaagt aggactatct gaggaagaat tgatggatct
    70141 gcaacgcgct gctgaaatct ataaatttga tctagtaacc aacatggtag gagaattccc
    70201 tgaactacaa gggatcatgg gagaaaaata tgctctgctt aaaggcgaga aaccagccgt
    70261 agcaacagcg atccgtgaac attacatgcc tatatccagt gaaggagatc tacctgagac
    70321 tacagtaggt tctgttttag cgattgctga taaattagat agtatctttt ctttctttgc
    70381 agttgggatg atcccaagtg gctcaaacga tccatatgct ttgcgccgtc aagcatatgg
    70441 tgtcgttcga atcatcgaat cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac
    70501 gattagtgaa gttatcagta aagatacgga tcgttttggc attggcttgt cagcgggaca
    70561 acaacaggtg atcgacttta tcaaaggccg tctgcgccaa ttattgacaa ctaagaatat
    70621 tcgtcatgat gtcatcgaag ctgttttgaa cgcagaacaa aaagatctga caaaagtatt
    70681 tgcagcagca caattgttca agcagcatct tgcagatgaa gacttcaaac catcgatgga
    70741 agctttgaca cgtgtcgtga acttagcgaa aaaagcggag cttgaaaaac aatcagaagt
    70801 agaccctgaa ctgttcgaaa acgaagcgga gaaagaactg cacaaagcag tagaggcaat
    70861 gaaaacaagc tttgctgacc aaacgatcaa cgaaaattat gatcagcttg taagccttcg
    70921 cccactgatc gaaaattatt ttgatcagac gatggtcatg gcagaagaac cagcagtacg
    70981 taagaaccga ttaacgcaat taagtcaaat tgctgccatg gctttgtcac tagcaagttt
    71041 agatcaaatc atcacaaaat aatttctaat atgtaaataa accagagaac aggcacaa
//
LOCUS       DAXLPI010000002        59388 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXLPI010000002 DAXLPI010000000
VERSION     DAXLPI010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.002"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            50..2491
                     /locus_tag="ACF3T5_000070"
     CDS             50..2491
                     /locus_tag="ACF3T5_000070"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFD3630714.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2821..3525)
                     /locus_tag="ACF3T5_000071"
     CDS             complement(2821..3525)
                     /locus_tag="ACF3T5_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630715.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3712..3957)
                     /locus_tag="ACF3T5_000072"
     CDS             complement(3712..3957)
                     /locus_tag="ACF3T5_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630716.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(3974..4825)
                     /locus_tag="ACF3T5_000073"
     CDS             complement(3974..4825)
                     /locus_tag="ACF3T5_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFD3630717.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            4991..5995
                     /locus_tag="ACF3T5_000074"
     CDS             4991..5995
                     /locus_tag="ACF3T5_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFD3630718.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6236..6592)
                     /locus_tag="ACF3T5_000075"
     CDS             complement(6236..6592)
                     /locus_tag="ACF3T5_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFD3630719.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            6960..8147
                     /gene="tuf"
                     /locus_tag="ACF3T5_000076"
     CDS             6960..8147
                     /gene="tuf"
                     /locus_tag="ACF3T5_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFD3630720.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8335..10926)
                     /gene="mprF"
                     /locus_tag="ACF3T5_000077"
     CDS             complement(8335..10926)
                     /gene="mprF"
                     /locus_tag="ACF3T5_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFD3630721.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(10952..11713)
                     /locus_tag="ACF3T5_000078"
     CDS             complement(10952..11713)
                     /locus_tag="ACF3T5_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD3630722.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            11880..12512
                     /locus_tag="ACF3T5_000079"
     CDS             11880..12512
                     /locus_tag="ACF3T5_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFD3630723.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            12571..13431
                     /locus_tag="ACF3T5_000080"
     CDS             12571..13431
                     /locus_tag="ACF3T5_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFD3630724.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13616..15931
                     /locus_tag="ACF3T5_000081"
     CDS             13616..15931
                     /locus_tag="ACF3T5_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFD3630725.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16113..17954
                     /gene="dnaG"
                     /locus_tag="ACF3T5_000082"
     CDS             16113..17954
                     /gene="dnaG"
                     /locus_tag="ACF3T5_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFD3630726.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            17978..19087
                     /gene="rpoD"
                     /locus_tag="ACF3T5_000083"
     CDS             17978..19087
                     /gene="rpoD"
                     /locus_tag="ACF3T5_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFD3630727.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19304..21445
                     /locus_tag="ACF3T5_000084"
     CDS             19304..21445
                     /locus_tag="ACF3T5_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFD3630728.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            21641..22504
                     /locus_tag="ACF3T5_000085"
     CDS             21641..22504
                     /locus_tag="ACF3T5_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFD3630729.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22609..23082
                     /locus_tag="ACF3T5_000086"
     CDS             22609..23082
                     /locus_tag="ACF3T5_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFD3630730.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            23178..24065
                     /gene="xerD"
                     /locus_tag="ACF3T5_000087"
     CDS             23178..24065
                     /gene="xerD"
                     /locus_tag="ACF3T5_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFD3630731.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            24084..24455
                     /locus_tag="ACF3T5_000088"
     CDS             24084..24455
                     /locus_tag="ACF3T5_000088"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3630732.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            24452..25222
                     /locus_tag="ACF3T5_000089"
     CDS             24452..25222
                     /locus_tag="ACF3T5_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFD3630733.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            25206..25775
                     /gene="scpB"
                     /locus_tag="ACF3T5_000090"
     CDS             25206..25775
                     /gene="scpB"
                     /locus_tag="ACF3T5_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFD3630734.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            25781..26497
                     /locus_tag="ACF3T5_000091"
     CDS             25781..26497
                     /locus_tag="ACF3T5_000091"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFD3630735.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      26579..26703
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            26826..27437
                     /locus_tag="ACF3T5_000092"
     CDS             26826..27437
                     /locus_tag="ACF3T5_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFD3630736.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            27815..29338
                     /locus_tag="ACF3T5_000093"
     CDS             27815..29338
                     /locus_tag="ACF3T5_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFD3630737.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            29575..30915
                     /locus_tag="ACF3T5_000094"
     CDS             29575..30915
                     /locus_tag="ACF3T5_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFD3630738.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            31069..31806
                     /locus_tag="ACF3T5_000095"
     CDS             31069..31806
                     /locus_tag="ACF3T5_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD3630739.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(31971..32192)
                     /locus_tag="ACF3T5_000096"
     CDS             complement(31971..32192)
                     /locus_tag="ACF3T5_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFD3630740.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            32223..33254
                     /locus_tag="ACF3T5_000097"
     CDS             32223..33254
                     /locus_tag="ACF3T5_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3630741.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            33241..34644
                     /locus_tag="ACF3T5_000098"
     CDS             33241..34644
                     /locus_tag="ACF3T5_000098"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFD3630742.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            34710..35345
                     /locus_tag="ACF3T5_000099"
     CDS             34710..35345
                     /locus_tag="ACF3T5_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFD3630743.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            35394..36074
                     /gene="cmk"
                     /locus_tag="ACF3T5_000100"
     CDS             35394..36074
                     /gene="cmk"
                     /locus_tag="ACF3T5_000100"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFD3630744.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            36209..37438
                     /gene="rpsA"
                     /locus_tag="ACF3T5_000101"
     CDS             36209..37438
                     /gene="rpsA"
                     /locus_tag="ACF3T5_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFD3630745.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            37620..38930
                     /gene="der"
                     /locus_tag="ACF3T5_000102"
     CDS             37620..38930
                     /gene="der"
                     /locus_tag="ACF3T5_000102"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFD3630746.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            39173..39448
                     /locus_tag="ACF3T5_000103"
     CDS             39173..39448
                     /locus_tag="ACF3T5_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFD3630747.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            39683..40729
                     /locus_tag="ACF3T5_000104"
     CDS             39683..40729
                     /locus_tag="ACF3T5_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630748.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            40731..41990
                     /locus_tag="ACF3T5_000105"
     CDS             40731..41990
                     /locus_tag="ACF3T5_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFD3630749.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            41996..42577
                     /locus_tag="ACF3T5_000106"
     CDS             41996..42577
                     /locus_tag="ACF3T5_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFD3630750.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(42659..43534)
                     /locus_tag="ACF3T5_000107"
     CDS             complement(42659..43534)
                     /locus_tag="ACF3T5_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFD3630751.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            43682..44014
                     /locus_tag="ACF3T5_000108"
     CDS             43682..44014
                     /locus_tag="ACF3T5_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFD3630752.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            44126..45334
                     /locus_tag="ACF3T5_000109"
     CDS             44126..45334
                     /locus_tag="ACF3T5_000109"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFD3630753.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            45488..45943
                     /locus_tag="ACF3T5_000110"
     CDS             45488..45943
                     /locus_tag="ACF3T5_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630754.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            46305..46580
                     /locus_tag="ACF3T5_000111"
     CDS             46305..46580
                     /locus_tag="ACF3T5_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630755.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            46593..48671
                     /locus_tag="ACF3T5_000112"
     CDS             46593..48671
                     /locus_tag="ACF3T5_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFD3630756.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            48834..50717
                     /locus_tag="ACF3T5_000113"
     CDS             48834..50717
                     /locus_tag="ACF3T5_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFD3630757.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            50729..51676
                     /locus_tag="ACF3T5_000114"
     CDS             50729..51676
                     /locus_tag="ACF3T5_000114"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFD3630758.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            51696..52223
                     /locus_tag="ACF3T5_000115"
     CDS             51696..52223
                     /locus_tag="ACF3T5_000115"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFD3630759.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(52286..52939)
                     /locus_tag="ACF3T5_000116"
     CDS             complement(52286..52939)
                     /locus_tag="ACF3T5_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFD3630760.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            53071..53913
                     /locus_tag="ACF3T5_000117"
     CDS             53071..53913
                     /locus_tag="ACF3T5_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFD3630761.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            54071..54967
                     /locus_tag="ACF3T5_000118"
     CDS             54071..54967
                     /locus_tag="ACF3T5_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFD3630762.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            54970..55683
                     /locus_tag="ACF3T5_000119"
     CDS             54970..55683
                     /locus_tag="ACF3T5_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFD3630763.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            55703..56221
                     /gene="msrA"
                     /locus_tag="ACF3T5_000120"
     CDS             55703..56221
                     /gene="msrA"
                     /locus_tag="ACF3T5_000120"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFD3630764.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            56218..56445
                     /locus_tag="ACF3T5_000121"
     CDS             56218..56445
                     /locus_tag="ACF3T5_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFD3630765.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            56597..57148
                     /gene="lepB"
                     /locus_tag="ACF3T5_000122"
     CDS             56597..57148
                     /gene="lepB"
                     /locus_tag="ACF3T5_000122"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFD3630766.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(57231..57773)
                     /locus_tag="ACF3T5_000123"
     CDS             complement(57231..57773)
                     /locus_tag="ACF3T5_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFD3630767.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(58010..58408)
                     /locus_tag="ACF3T5_000124"
     CDS             complement(58010..58408)
                     /locus_tag="ACF3T5_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD3630768.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            58680..>59388
                     /locus_tag="ACF3T5_000125"
     CDS             58680..>59388
                     /locus_tag="ACF3T5_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3630769.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT        20133 a        10220 c        12384 g        16651 t
ORIGIN      
        1 atttgttttt acagcaaaga atggtatatt tttgtaggag gtggcttact tgaaagaagt
       61 cgttatgata gatgctgcaa gaacgccgat aggaaagtat cgcggaagtt taagtccatt
      121 tactgcagtt gaattaggca cacttgtgac taaaggatta ttagataaaa caaaactgaa
      181 aaaagataaa atcgatcaag taatattcgg aaatgtcctg caagctggta atggtcaaaa
      241 tgttgccaga cagattgctt tgaacagcgg tctccctgta gatgtcccag caatgacgat
      301 caatgaagtt tgcggatctg gtatgaaagc agtgatctta gcacgccagc tgatccaatt
      361 aggtgaagca gaacttgtga tagccggcgg aacagagagt atgtctcaag cacctatgct
      421 gaaaccgtat cagtcagaaa caaatgaata tggtgaacca atttccagta tggtcaacga
      481 cggattgact gacgcatttt caaatgcaca tatgggatta accgcagaga aggttgcaac
      541 acaattttct gtgagcagag aagaacagga tcgctatgcc ttgtcgtccc agttgaaagc
      601 agcacatgct gtcgaagccg gtgtattttc tgaggagatc atcccagtca agatttctga
      661 tgaagacgtg ttatctgagg atgaagcagt tcgtggaaat agtacattgg aaaaactggg
      721 cacgttacgt acagtattct cagaagaagg aactgtaaca gcaggaaatg cttcgccatt
      781 gaatgacggt gcctctgtgg tgatccttgc atccaaagaa tacgcagaaa ataataatct
      841 gccttattta gcaaccatca aagaagtagc ggaggtcggt attgacccaa gtatcatggg
      901 aatcgctcca atcaaagcca ttcaaaaatt gacagataga tcaggaatga atttatcaac
      961 aatcgatctt tttgagatca atgaagcatt tgcagcttct tctatcgtag ttagccaaga
     1021 actgcagtta gatgaagaaa aggtcaatat atatggagga gcaatcgcat taggccatcc
     1081 aattggtgca agcggtgcac gtatcttgac tacattagct tacggattgc ttcgtgaaca
     1141 gaagagatac ggtattgctt cattatgtat tggtggaggt ttaggcttgg cagtcttgtt
     1201 agaagcgaat atggagcaaa cacataagga tgtgcaaaaa aaaaagtttt atcagcttac
     1261 gccatctgaa cggcgcagtc aattaataga aaaaaatgta ttgacacaag aaactgcgtt
     1321 gatttttcag gaacaaacac tttctgaaga gctctccgat cacatgatcg aaaaccaagt
     1381 gagtgaagtg gaaatcccaa tgggcatcgc gcagaatttt caaatcaatg gtaagaaaaa
     1441 atggattccg atggcaacag aggaaccatc tgtcattgca gctgcaagta atggtgcaaa
     1501 aatctgtgga aatatctgcg cagaaacacc tcagcggctg atgcgagggc aaatcgttct
     1561 atcaggaaaa tcggagtatc aagcagtgat caatgcagtt aatcatcgaa aagaagagct
     1621 gattctttgt gcaaatgaaa gttatccttc aatcgtgaaa cgcgggggcg gtgttcaaga
     1681 tatctctact cgggaattca tggggagttt ccatgcatac ttatctattg attttttagt
     1741 ggatgtaaaa gatgcaatgg gcgctaacat gatcaactcg attcttgaga gtgtggcaaa
     1801 caagttacgg gagtggttcc ctgaagaaga gattctattc agtattttaa gtaattttgc
     1861 tacagaatca ttggcttctg cctgttgcga aatcccattc gaacgcttag ggagaaacaa
     1921 agaaatcggt gaacaaattg ccaaaaaaat ccaacaggca ggggaatatg cgaagcttga
     1981 cccttaccgt gcagcaacgc acaacaaagg gatcatgaat ggtatagaag ctgtagtggc
     2041 agctacagga aatgatacac gtgcggttag cgcttcgatt cacgcctacg cggcaagaaa
     2101 tggattatac caaggattaa cagactggca gatcaaaggg gataaactag ttggaaaact
     2161 aacggtgcct ttggctgttg ccacagtagg cggagcttcc aatatcctgc caaaagcaaa
     2221 agcatcttta gctatgttag atattgattc agccaaggaa ctagcacaag taattgctgc
     2281 agtagggtta gctcaaaacc tggctgcttt gcgtgcatta gttacagaag gaatccaaaa
     2341 aggccacatg ggacttcaag ctcgttcatt agcgatctca attggggcta ttggtgagga
     2401 aatcgagcaa gttgcgaaaa aattacgtga agcagaaaag atgaaccagc agacagctat
     2461 acaaatattg gaaaaaatcc gggaaaaata aaaaagcaat atctttagaa gaataaagca
     2521 agatttacag ttattttatt tactgcaaat cttgcttttt tctctatcag aaaatatgtt
     2581 tattattaga attttttata gttaaggtta ggcatcttaa gaaatcaggc aactccaaga
     2641 tatcttcagg tagaagagaa gtcgcctaac attgtaggat cataggtgat ttgttggaca
     2701 ggtcaagctg atatttgagc ctgatgttag aaggaatttc tttttacttg ctgtagaaat
     2761 tattatatga ctaaaaaggc tgggatttta aatcccagcc ttttatgaca aatagaattt
     2821 ttatcgatca gtaatcgctg tcgctgcaaa ttgttcgatc aaaggaatca aagtcaattt
     2881 gactttgtat ttccgttcga gtgctttttc gataacgaac tcattttcag tagtcataga
     2941 aatagttgct aatttacgag gttcatcagc atttttttca ttaataacta cttcatgttc
     3001 attttgttct gtgtaaaatt taggatactc agcaacggct tttgaaaaat cagcaaaact
     3061 taaagcggca ttcgtatcag ttgagcgtgt attttcagtt gtgtccgctt ttggtttttc
     3121 cgttttttct tctggcacac tgactgtttc ttctgtaact ggtgtcgcaa cagcttgcgc
     3181 agttggttca tcgtttttca atcgttgatc attccagtaa gacacgattt caataagaag
     3241 attgtcacgg aatcgttcga aaatcccgtt ttctcctgaa cgaattctct ttaatacagt
     3301 attgaagttg tcatcgattg gcacgttgat ccgtgtatct tcatttgttt tcccagctaa
     3361 tccgatcgtt aagatttgac ggtttttggg atcaagcatg acttgtaacg gcaaaccata
     3421 gcgtcgcaag ctctcttctt ctagagaacg gaataattgt cctcttacac catccaatga
     3481 aggacggaat tgatttaata atttttgttt ctgttttttc ttcatatgtt ctctccttcg
     3541 ctcataccct ttcgattata agaaaaaaac agccaaatga aaaggagtaa tgcaaaaaaa
     3601 cttgcgtata cgcaaattac aacagaaaaa tttgcgtatt ttcccgtttt ttgaaagaaa
     3661 aataaaaaat ggaacggatt atgtatatat acataatccg ttccattctt tctatttttt
     3721 cggtatcaag gcaaaaacag gtagttcagg tgaataaatg acttcatgtt cgtgccaggg
     3781 tttgaccatt agcgaatgat cgaaccgttt tttcagttct tcgatgattt gatcatgtgt
     3841 ccagtttctt gcaggaaaat gttggatttt ctctgcatcg atcaaaaaga caaaatcgga
     3901 atgaaaagtc tgggtaactt cttccgagag agaattcaag cgttcgattg gatccataaa
     3961 atcacctttc gacttaaaat tgggtagtat ctgggtcttt tgctttgccg actacaccat
     4021 ctaactgatc gatttgtttc atttcttctt ctgttaattc aaaatcaaat aattcagtat
     4081 tttctttgat tctgcttggt gtcactgact taggaagagg aaggaattcg tgttgcagcg
     4141 accaacgcaa agcaacttgt gcaatcgtct tgtcatgtgc ttcagcgatt tgtttcattt
     4201 caggcacatc gaagatttta ccagtgccta atggactata tgcttcgagg atcatatcat
     4261 gtttttttgc atactcaact acagttggct gtaattcgcc tggtgcaagg aaaacttgat
     4321 tgaccatcgg cataattttt gctgtttttg ctagtgtatc tagatggtgg ggtaagaaat
     4381 tgctcacacc gattgctttg attttacctg cttcatacag ttcttcaaat gctcgccatg
     4441 tatctgcatt tgcttgttcc caattatcac gaaaagcaac agggtttggc cagtggatca
     4501 aaaacagatc taaataatct gtttggagct tcttcaatga ttcttcaaaa gaagacatca
     4561 caagttcata gctgtggttt gcattccata attttgttgt caagaatatc tcttctctag
     4621 gaattccact ttccttaatc gcttggccga cactttcttc attcttgtat ccttgggcag
     4681 tatcgatatg gcgataacct gcagccagcg cttctttaac agaggaaaca gcgacatccc
     4741 catcaggtgt ttgccatgtg ccgaatccta ctaccgggat ttcgtaacca tttaatagac
     4801 gataagtgct tgttaatgaa ttcatacttt cagctccttt tttattcgtt caactataag
     4861 tgtagcgcat ttttcgaaaa aaagaatcca ttttgtttct actcttgaaa tttttgttta
     4921 aaagaaaatg attttgattc tggtattttt gatttttttt ccgtaaacta agacagaagg
     4981 gaatgagaaa atggatatct atttaaaaaa agcaattctg catattattg acagagaaac
     5041 tggttctcca gtcttttcac aaaaagagct ggatctgaca aaagaatata ttcgtgattt
     5101 tcttcaaaag aagatccaaa aaatctcttc tgctcaaaca aaaactggac aactagcaga
     5161 agaaagcatg atcgttgaat cacttaaaat cgctcaagaa aattttgtgg aagcaagtga
     5221 agcaatcgtt cagcggtggt tcgatatcta ccaagaaagt gaagaagcac ctagtgcaga
     5281 tgtatttgtt gttttgtatg aattagatac agtgatgtac ttatctttat tgaaagtcaa
     5341 ttatcgtgag gcttacactc actttgttga agcagacgaa gcagggatcg acaaccgctt
     5401 gattttgaat cgagcaattc taggggcgaa aacacaaaaa gcagatgaag cattcgctgt
     5461 gaatttgact gattggacct acgaattaat tgaaaaaaaa tatgagtttt ctggcaaaaa
     5521 agagctttat ttttctggaa gagtgatcga aagtgttcct gcaccttctt tagaagaaaa
     5581 tgtaaaagtg atccaaaaag tagccaagca tcttggaaaa aaatttgaga cagaagaatt
     5641 tgatatcatg gctgatttga aagaagctgt ctatgatacg atagaagaaa aaggtcggtt
     5701 ggatcatgag atgattgcgg aaaaagtctt caaggaaaac atcacggcaa aaatggcttt
     5761 taaagaagaa gtacaagagc aaggatttgt cccagaagca cctcctgtaa aagaagtaca
     5821 agaaatttct gagaaaaaat ttggaaaaca aaaattcaag ctatcaaacg ggatcgaact
     5881 gatcgtacca gtagatgtat atcgcaaccc agatttgatt gaatttgtga ataatccgga
     5941 tggtacgata tccgtgatga tcaaaaatgt ggatgaagta ttgaatcgtt tataaaaatg
     6001 actagaacat taggtgttca agtaccagtg atcagttaaa tgaattgtgt caagagaagg
     6061 atcggaaaag acgaatataa caaaaaaaga gcgtgagaca aaagtaaaga acacttttgt
     6121 ctcacgctct aaacacgtat aaacggcgga agcagaagca actccttctt atttcttgga
     6181 gttaaacgct tctgtcccag cctcttttta tctaataaaa ttttaagacc atttactaat
     6241 tatcgatttt ttctgccgca tttttaggca cttcagaagc ctttatttct tcccagctta
     6301 atacaccttt acgaggattg actttgagtt taagatacgc attcatcttt aatggatgtt
     6361 ctctttcttc gcgaagcgtc acctgttttt tttcaccatc tttattataa gccgcttgct
     6421 tgtaggtata agtcgtgaat ttctctccgt tatctgcttg ggtagctgac atttcgccat
     6481 tcgtcgtgat ttttgtataa taagcttcgc cgccgtaaaa ataatgataa gctgcaaaac
     6541 ttccgccgag aaatatgcct aaaacgacaa gtacgccaat taattttttc atgtacttca
     6601 cctctttcct tattattaac gaaatagaag atttcgtcta ttgaaagttg ttggcaacag
     6661 ctgaatttta aacttttcct cattttgttc ttattgcttc aaaagaaagt taaggactat
     6721 cttgaagttt ttctcaaaaa acacattttt ttatttataa aaggtacttt tttcgcgtat
     6781 aagacaagcg agtgctctta gttgactttg accaatattg acgtataata agcttagttc
     6841 gaatcttaca aaaagtaagt cggacgtatt ctttttgatt tagtcaaaaa aagactataa
     6901 taaaaaggtg agagctgaaa tttttcagtg atctatatta tttaggaggt ttgtaaatta
     6961 tggcaaaaga acattatgac agaagtaaac cacacgttaa cattggtacg attggacacg
     7021 ttgaccatgg gaaaactact ttaactgctg cgatcactac agtattaggc aaaaaaggcc
     7081 tagcaaatcc tcaagactat gctagtattg atgcagctcc agaagaacgt gaacgtggga
     7141 ttacgatcaa taccgcacac gtagaatatg aaacagaaaa acgccactac gcgcatattg
     7201 atgcaccagg acacgcggac tatgtgaaaa acatgattac aggggcagca caaatggatg
     7261 gtgcaatctt agttgtttct gcaactgacg gtccgatgcc tcaaacacgt gaacacattt
     7321 tattgtcacg ccaagttggt gtaaaatacc tgattgtttt cttgaacaaa gttgatttag
     7381 tcgatgatga agaattgatc gatttggtag aaatggaagt tcgcgagtta ttgagcgaat
     7441 atggtttccc aggcgatgac actcctgtga tcaaaggttc cgcattaaaa gcattgcaag
     7501 gcgatccaga tgctgaagct gctattatgg aattgatgga tacagtagat gaatatatcc
     7561 caacaccaga acgtgataca gataaaccat tactattgcc agtggaagac gtcttctcaa
     7621 ttacaggtcg aggaactgtt gcctcaggtc gtattgatcg tggtgctgtt cgtgtcggtg
     7681 atgaggtaga gatcgtaggg atcaaacctg aaacacaaaa agcagttgta acaggtgtag
     7741 aaatgttccg taaaacgtta gattacgggg aagctgggga taacgtaggc gtgttgttac
     7801 gggggatcca acgtgacgat atcgaacgtg gacaagtgct tgctaaacca ggttccatta
     7861 ctccacatac aaaattcaaa gcagaagtgt acgtgttgac aaaagaagaa ggtggacgtc
     7921 atactccatt cttcaacaac tatcgtccac aattctactt ccgcacaact gatgttacag
     7981 gaacaatcac attgccagaa gatacagaaa tggtcatgcc tggtgacaac gtgacgattg
     8041 atgttgactt gatccatcca atcgctgttg aaaacggaac gacattctca attcgtgaag
     8101 gcggacggac agtgggttca ggtatcgtta cagagatcga agcataagaa acgcgactcg
     8161 aatattttgg taatgaaaaa tgtttaaaat aaaagagcgg tccaaacagg atttctgttt
     8221 ggaccgctct ttgttcttca attgtataag aagagattgt agaagcatga actttcgttt
     8281 tacttcagtc tcttttattc atcatgagag tgttcatcta tcggtatgca gtttttaata
     8341 ctttcttcgt atccattttt tcaaaccact tattctacct tgtgtctgct caactggcgc
     8401 attatcaata atcagcaaag caatcataac atatgcaatc caactatctc tagaatatag
     8461 agtataacgt gagacccagt ttgtagcgta cttgctttta tattctctta atccatggaa
     8521 agaataaaaa tgagaaccaa actcgtaaac caagtatgca atcctttcct gtaaaaaact
     8581 ctttctggaa gttccgacat ttgaaagagg ggccatccct aaattgaacc attggatatg
     8641 ttcttctttc atataggcaa acaaatgcaa gaacaagaaa tccatactgc cagaaggtgc
     8701 ttttttagga tcatatcgca tcagatcaat cgttcctact ttattgttcg tgtaagtagg
     8761 catgatcgtt gcaaaagcga caatttcttc ttgggtattt ttgacaactg caataggcgc
     8821 tctttgtaga taatcttcag agaaaaagcc taaagaaaac cctttttcct ttcgtgaacc
     8881 caaccaatta tcagagatat ttttgaaagt actcatttgc tcagtagaaa atggtggttg
     8941 taagacatca aatgtaaatc cttctcgttc gatccgattc aagacagcac gagtgccctt
     9001 catttttttc cctgaagttg taaaactatt caggtctaca taagcttctt cccccatctt
     9061 aatgaaatcg tagccgaatt catgcaaaat cataacaatc tcttcgcttg tttcgtagaa
     9121 aactggtaaa tagcatagtc gatctgtttc ttcgataaaa gcttcgattg cttctggaaa
     9181 atcttctttc ttaccagaag gatcacccat tacaatacat ttattattga agcaagcgaa
     9241 ttggagaaga acagttggtt caccgtcttt ttcataggca aacatccgct tatcttttaa
     9301 gaagatcaat tgactatcgg agtttcctcc ataagttgta aggatcttca gggccttttc
     9361 ttcattaaag gcttcgccaa tctgtttctt ttctccttga aggaaatgaa caaatagaac
     9421 gatcatgaaa ctaacagcga tgatggccaa caatcccgaa aaccaaatct tttcagaagg
     9481 aaataagaaa aaggaaataa aatggtgacg ccgatgaggg aaatccggca gattgtatac
     9541 cccaataacg atgtaaagca aggacagtgc accaataatg attccatcga tcgtacgcca
     9601 ctcccatgaa tagatcaatt gttcccgaaa taattcgttt ttagaagcaa gaatgatcag
     9661 aagcagaata gacaagaaaa ccaccggtgt gaaactaaaa tcacttaata aaacataaag
     9721 taaagctaga acaatcaaga agattgtagg caaatatgct cgtttcacac gtgctgcgat
     9781 ccctctgccc ataatgatta aaagaaatcc caatagtata ctaggaaatt ggataataaa
     9841 atgaaatttc aatgggttca aactatgaag ccagcgaaac tctgtaaaag cttgcgggat
     9901 agtggcagaa agtactagca tgattccaga aaagtataat aagacaacca cgattttatg
     9961 cgcaatttct gttgctaatt gtttagggat accagaataa cgctgatcaa aagtggaacc
    10021 gatatttttg aagaaaaaga cgattccaat caaaaacgga atgatgtagt agaacaggcg
    10081 atagagcaag atccacacaa cgactatttc tcttggcaca ccaatagcgg acaacccgat
    10141 aatcatcatt acatcgaaac tcccaatttc tcctggaatc atagacacga ttcctatcac
    10201 tgaagcagca atgaataacg gaattgtttg ccacagatca atccggattt ccattaaata
    10261 accaacagaa aggaaactaa acaggacacc ggtccattcc aacaatgaaa ctagcaatag
    10321 ctgtccttga gttttaagtg aggtaccacc aaggaatcct ttctttttga aagtagtaaa
    10381 taaaaagacg acaggaaaat atagaccgcc gccgatcaac catatccaat attgcttcaa
    10441 aaaaggagca actggcgtga agactacaag taaaaaagaa agcagactgt aaatagaaag
    10501 tccagccatc aaaaacaaga aaactttcga taatgcttgg atcacgtttc tgctctcttt
    10561 cttttgtcca tatagttcag agcgaagccc cacactaaca aacccgccga aaccggcaat
    10621 attgttgatt gtattgatca gccagcttgt ctcaaacaag taacgaggat tctgcttttg
    10681 ttcaagcatc cgattcaaaa taatatcata accaatcata ggtaacacag agacaagacc
    10741 aatcaacagc ataagtccgc ttttccatac aggtatagtg gcaaacgttt ctcctaattg
    10801 ctggatagat agagttttac cgatggacat caattcaccg acaataatga caatgacaga
    10861 aatcaaaaag attgttttga acagtcctag gtggtttttc agccattgta tggtattttt
    10921 taacaaataa atcaccttct attcctaata gttatgtttc agaaagaaat tgaagaactt
    10981 ctcgattgaa gcgttcagga tcttttctag ctaattcatg tccttgttct ttcacaagca
    11041 caaaggaagc ttttggtata gttttagcta tatacaaaga atgttttagc ttgattacat
    11101 ctttttttcc cacgatgatc aaagtggggg agttgatttt ttttaaatca ttttctgtaa
    11161 gcccgatgtc atgcagcaaa agtttgataa ctaaaagatt tttgcgaaga cttgggcgaa
    11221 ataaagagag taaccatacc catgcataat gaatattact gatgactctg gcagaaaaac
    11281 gaacgccttt aacaagcgtg tttccagaat ttaaaatcaa tcgatgaact tttttaggga
    11341 agctgctggc aaataccagt gctagatttg ctccatcact aaatccaaga aaatctgcct
    11401 gatcgatttt ttccagcaac atgatcgtat tcagatcctc ggccattaac tgaaaattca
    11461 acatagatgc ttcgtttgtc gaacgaccat gtcctcggct atctactaga tacacggtat
    11521 agtagcgttc gagtacaggg acctgttctg aaaaaaagcg gccgctgccg tcattgccat
    11581 gcagtaaaaa gagagggaag ccttgaccgc tcttttcata gtaaatttcg ctcccatctg
    11641 gcatttttgc atatttcttt tcttttttca cataatcacc tactgtgtaa agtataacga
    11701 ttatatcagt cattgggcaa ttgtttttac tgaaaaacaa cgatcatttt cgtaagatga
    11761 caaaaaatac aggaaaattc attttttctt aaggcgtaca cagaaatcaa ggaagaaata
    11821 gccaagaaga taaagaaata gtaagataaa aaaagatgtc aaaataacta ggtgatgaaa
    11881 tgaagcgaaa acgattatct attatccgta ttctttttta catgatcatt ctgacggtta
    11941 ttggtgcggt gttgttttta ggaggaagaa cttatcgttc ggtaaaacga gtggaaagct
    12001 atcaaataga aataacaaca gctgcaaaaa attatcatat ggaaaactac gagaatttga
    12061 tcaaggcgat aatattgacc gaatcaaaag gaaaagggac agatctcatg cggagctcgg
    12121 aaagtctgca tggagaagct gagatcattg ataatccagc tgacagtatt gatcaaggtg
    12181 tcaaatattt ggcggaaatg atagtagaag caaagaaaca aggatgcgat ttatggacag
    12241 ctgtccaagc atataatttt gggaaagatt atattgccta tgtaaaaaat catggcggag
    12301 aaaacacagt agaattagca gaagcgtatt ctaaagatgt gctgagccca cttttaggaa
    12361 atgaggaagg gaaacaatac cgatattggc ggatccagtc cttgctttac aatggcggct
    12421 atctctatca taatggaggt aacatgtttt atcctgatat tgtcaaaatg aatgaaaaaa
    12481 taatagagac ctatgaaaaa gtttttaagt aaaatagcag gaaatgttca atcatgaata
    12541 aaattaaatg aaagaaggag tgcgcaaata gtggaaaaaa ggcaaaaaat ggaaaatcaa
    12601 aaagtggggt acacaaccaa agactggatg ttacggtttg tgaagggaat gtttatcggt
    12661 tcaggattta tcttgccagg agtcagcggt ggtgcattag cagctgtctt tggcatgtat
    12721 gagcggctga tttctttttt agcccacctt acaaaaaatt tcaaagaaaa tgttttgttt
    12781 tttctgcctg ttggtctagg cggagtgaca ggcatctttc tactttcttt tttagtcagt
    12841 tttttattag gtgcgtatga aacaacgata ttatggtttt ttgtcggatg tatcgctgga
    12901 actgttcctg cgttatggaa agaagcaggg aaaaaaggaa gagatacttc tgatgtgatc
    12961 atcatggttg ttacttttgt tctggcttat ttgtttttat tatacggcgc tcaattattt
    13021 gacgggcaag tcgatcagaa ttttggaaca tggctgattg ccggcggatt gattggtttg
    13081 ggtatgatcg tcccaggatt gagcccttct aattttttgg tgtatatggg attgtataaa
    13141 gcgatggctg acggatttaa agaagtacga cttgaagtga ttgtgccgat cggaattggt
    13201 gggattcttt gtgttcttgg attgtcaaaa atcatggatt acattttctc aaaggcatat
    13261 agcaagctat tccatttcat tttaggtgta gtttttgctt caacgattat gatcattcct
    13321 accgattacg cgggattggg agttgctggg gtactagctt gtcctgttct ttttatagcc
    13381 ggtacagcac taggttggtg gatgagccgt ttggaggaac aatataagta aagatagatt
    13441 gataataaaa acagcacacg ggtgattcaa acaaaaaggt tggcatcttt tttgtaaaat
    13501 cattcgtgtg tttttctata aattttataa aaacaagttg tttttaatct tggaacacgc
    13561 tagacttaaa cacgtgatta tttttgaaag ttggcggaaa atggagaaaa agaaaatgaa
    13621 tggacttaca cagcaagagg tagttcagaa aatggcttct ggccagcaaa atgactacca
    13681 ggaagatgca tcaaaaagta caaaacaaat attcagcgat aatatattga cgttattcaa
    13741 ttttctgaac tttggtattg ggatttgtct gttatttgta ggtgcgtatt ccaacatggc
    13801 gtatttagca attattgtcg tcaatatcgt tattggaatt tatcaagaaa tacatgcaag
    13861 aaatcttgta cggaaattat cgattgtttc ccaatcagaa gtactagtga tccgggacgg
    13921 taaagaacaa tcgatttcta ctaaagagct ggtattagga gatttggtaa aaatcggagc
    13981 aggagaacag ataccagccg atttacaggt tatatctgga cgggcagaag caaatgaagc
    14041 tcttttgaca ggcgaatctg atttgattgt caaagaaact ggagctgaat tgctttctgg
    14101 aagctttttg acaagcggtt ctgtcatggc tgaagttatc cgagtaggtg cagataatta
    14161 tgctgtccgc ttgactagcg aggcaaagac tcacaaagct attcgttctg aacttgtaga
    14221 ttcgattcgc aaagtatcca aattcactag tttcgtgatt attcctttgg ggatcatact
    14281 atttttggaa gcactgttca tccgcgatgc ttctatcaaa atttctgtta ttgcatctgc
    14341 agcagctttg ttaggcatgc ttccaaaggg tttggtattg ctaataagta ttgcattaac
    14401 gacaggggta accaaactgg caaaaaaacg aatccttgtt caagacatgt attcaattga
    14461 aacgctggcc catgtcgata cattatgctt ggataaaaca gggaccatta ctgaaggaaa
    14521 aatgagtatc cagcgggtag aagcactaca tccgctgtac gagaaagcca tcccagaaat
    14581 tatggggacg tatctggcag aaagcagtga taacaacatc actatgcggg ctatgagaga
    14641 ttattttgag ctttctaatc ggtatgatgt gacaaacaaa gtcgcatttt cttctgatcg
    14701 caaatggggc gcgatggaat ttccagaact gggagtcgtg tacgtaggag cgcctgaacg
    14761 tttggtggaa gaagatcggc ttccggacag tctaaaaaca gcccaagcga atggctatcg
    14821 cgtacttatg ttagggatcg ctccaaatca gcaattgaac gaagaaaaac cagcatttgt
    14881 tgagccggtt gctctgtttg aaatcgatga tccaatccga aaaaacgctc aagaaacact
    14941 agcttatctt catgaggaag gtgtggatct gaaagtgatt tctggagaca atccttttac
    15001 tgtctccaat atcgctagac gtgcaggatt accgaattat gatgcgtatg tcgatctttc
    15061 tcaaattact gaagaaatgg aagtgcgaga agctgctcat cgctatacgg tttttggacg
    15121 agtgagtcct cagcaaaaga aattgctggt aaatgaattg aaagaaagcg gcagaacggt
    15181 tgcaatgaca ggcgacggtg tgaatgatgt tttggcgtta agagaagcag attgtagcat
    15241 tgctatggca gaaggtgatg gagcaacacg tcagattgcg aatctggtat tgttggactc
    15301 tgattttact acattgccag aagtcctgtt tgaaggacgc cgagtggtca acaatgtgac
    15361 gaaagtctca ggcatctttt ttatcaaaac gatttattca tttattttat cgattatatg
    15421 tgccgttaca gcaattggat ttccgtttat ccctatccaa atcacgctga ttgatttagc
    15481 gattgaaggg tatccttcct tctttttatc ttttgaacaa gataaaagaa aaatcactta
    15541 ccgttatttg cctacagcac ttattaatgc tttaccaaat gccttgcttg tagtagtgaa
    15601 tatcattgcg gtgtatctac ttggaaaaac attcgcatgg ggaaatgctg aaactactac
    15661 tttgatgtat tacttgctga ttgggatcag ttgcatggca gttgtaaaag cttgttatcc
    15721 cttcaaccca ttgcgcatct tcctagcagt aacgacgatc gtagggatat atgtagctgc
    15781 aatgctgttc catggaattt tggaagtaaa cttagggttg ggagagagcg taggatactt
    15841 ctttgtattc atgctcgtaa atattgttct tcgagtattg tactggaagg ctgatattca
    15901 aaatcagtta ctgaaaagag tagcaaattg atgcttaatg agatagaaat gtgttatact
    15961 tgaaaaagca atgaaatatg atacaatcaa acatgtataa ttgtgtcctt taaaatcgca
    16021 ctgatttcag ttagtgcgat tttttaccgg ttattctcct gaaatatctt ctgttaaacc
    16081 caaaagatat caatagagca gggggtggaa aaatggctca acggattcct caagaggtta
    16141 tcgaaacagt acgcagtcaa acgaatatcg ttgaggtgat tggacaatat gtccagttga
    16201 aaaagtctgg taaaaattac ttggggctgt gtccttttca tgaagaacgt acaccgtctt
    16261 tttctgttgc agaagacaag cagatttttc attgctttgg ttgtggtaag ggcggaaacg
    16321 tgtttacttt tcttcaagaa ctagaaggtc taagttttcc agaagcagtg gtaaaagtgg
    16381 cagacttcga acagataccg ttagaagatc aatggcgtca gcgtactata gctgtccaaa
    16441 atcctgaatc tgctacgggg aaactgattg ctgctcatga aaaagcagcg gaaatctatc
    16501 atcacatgct attgcataca aaagctggac agccagcttt agaatatttg caaagtcgtg
    16561 ggttgtcttt agaattaatt gaagaattca acataggttt tgcacctaat gagcgttctt
    16621 ttcttcagca agtattcaag aatgaatcaa tttcaaatga attgttagaa cgctctggat
    16681 tgtttgtcac gcatgatgat ggccgtatgt ctgatcgttt ttatcaaaga gtgatgtttc
    16741 cacttcgtaa ttctcaaggc cagacgatcg gtttttcagg acgctggctt gcacctgaaa
    16801 aagggcagga aaaagatcag cccaaatact tgaatagtcc agaaacagaa ctgttcaata
    16861 aaagacaagt tttatttaat cttgataaag caagaagcga tatccggaaa aacggagaaa
    16921 ttttgttgtt cgaagggttt atggatgtga ttgctgcatg gcaggctggt atcaaaactg
    16981 gattagcttc tatggggaca agtctaacag ctcaacagat ccaacaaatg gaaaagctga
    17041 cgaaagaatt ggtcttttgc tatgacggag acaacgcagg ttttgaagca accaatcgag
    17101 ggattgaatt gcttcggcaa aacagtcgct tagcactttc tgtcgtagtc gttccagaaa
    17161 agcttgatcc tgatgaatat ctccgtaagt atggagaagc atctttccaa gaattagtgg
    17221 ctcatgggcg cgaaacagtc tttactttta aatcacgtta tttaaaacaa gggaaaaatt
    17281 tagataatga aaaagacaag atcgaatacc tcgaagaatt actagttgaa cttagccgag
    17341 ttgtttcacc aatcgaacaa gatatgtatc tatctcaact gtcaacagag tttcagatcg
    17401 gcagagagac gatacaaaaa caactgcgcg atcttcgaag gacaaatcag caaaatcgtc
    17461 aagaacctca ggaaagtcaa cacactcagg ttacgagaca agaaactgca cggaaaaaga
    17521 gacctttgac gcaagtagaa aaggcagagc agattctatt atatcgtgca tttaaagaag
    17581 tttctgtccg taatatttta aaagaacgag aatttcagtt tattcatgat gcatatgcag
    17641 aagtttattt tttattcgat gcatatgtct cacaacatta tgaattcaac cttgcagagt
    17701 ttttagactt tttgcaagat gaaaatttaa ggcggttggt ggtggaaatc gaatatttac
    17761 cggtggcaga agaaagttcg ccacgagaaa tccaagactt gctagatgtg atcagcaaat
    17821 ctggcttagc tgatgaaata acattaaaaa aacaaatgca gcaagaagcc agacgaactg
    17881 gaaacaaaca gcttgagctt gaattgacga ttgaaatcat caatttaacg aaacagttaa
    17941 aacaagccaa atagctactc aaggggggcc ttcttttatg gcaaatggaa cagataaaaa
    18001 aacgtatgaa gcagcagtag cagctttcat caaagaaaac aaacccaaac gacaagttgt
    18061 atatgatgat ttatccaatc agcttgctac accattcact ttgaatgcag atgaaatgga
    18121 caaattgatc caaaagattg aagacgcagg tatcagtgtg gtggatgaaa atggtgatcc
    18181 atcaattcac agcttgaaga gtgctgagaa aaaagctgaa caagcaaaaa cagaagatct
    18241 atctgcgcca accggtgtaa aaatcaatga cccggttcgt atgtatctga aagaaattgg
    18301 ccgtgtctcc cttttaactg cggaagaaga agtagcgtta gcattgaaaa tcgaagaagg
    18361 cgaccaagaa gcgaaacagc gtctggcaga agcaaacctt cgtttagtag taagtatcgc
    18421 taaacgttat gtaggacgag gcatgcagtt ccttgacttg atccaagaag gaaacatggg
    18481 actgatgaag gcagttgaaa aatttgacta ccgcaaagga tttaagtttt caacctatgc
    18541 tacatggtgg attcgtcaag ccatcactcg agcaatcgct gaccaagcac gtacgatccg
    18601 tattccagtt catatggttg aaacaattaa taaattgata cgtatccaac gtcaattgct
    18661 gcaagacctt ggaagagaac ctacgccaga agaaatcggt gcggaaatgg atcttccaac
    18721 agaaaaagtc cgagaaatct tgaaaatcgc gcaagaacct gtttcacttg aaacgccaat
    18781 cggtgaagaa gatgactctc acttaggtga tttcatagaa gatcaggaag caacaagtcc
    18841 agcagaacac gcagcttatg agttactaaa agaacaattg gaagatgtac tagatacatt
    18901 gactgaccgg gaggaaaatg tccttcgctt acgttttggt ttagatgatg gccggacgag
    18961 aacattggaa gaagtaggga aagtatttgg tgttactcgg gaacgtattc gtcagatcga
    19021 ggctaaagca ttaagaaaat tgcgccatcc ttctcgttca aaacaattaa aagatttctt
    19081 agaataagca tgaactattg accagaagag aaatcagtaa aactgtttgg tttattttaa
    19141 aagaaaaaaa gcttgagact acgcagtctc aagctttttt ttctatagga aggaaattca
    19201 ttcattattt ttaagtaaat cctaaaaata agctattggc gtgacgtata gaatatgata
    19261 gattaaatgg tagcgtaaac ctagtaaagg agccgatcgc taagtgatga aaaaatgtcc
    19321 aaattgcgga tcgaaaaaaa tggagcagaa tactatctgt ccagactgcg gtcatcaaat
    19381 aactgcagaa gatcagcaaa taaaagaatc aattgaagaa acaaatacag atgcagctat
    19441 gcaggataaa tcaactgaag atgcagtctt tcctgataca gaattaaacg atccaatcga
    19501 atggtcggaa ttgaaagatt tacctctaga atctgtaatg gaattattcg aaaaagcaga
    19561 accagaagac gaagaaccac ctgctcacga tcaagtaact aatatagaga aaaatgaaca
    19621 gaagaaagca actgttcatg agacttcgga agaatcagga aagaatcaag aagaaacgct
    19681 tgaatcaaaa caaaaaagaa gagtagctga actgaaagaa atcgtcgata atgaggaaga
    19741 aaattcgata ctatccgctt atatcaaagc acatcgtgaa gatacaaaag aagagcacgc
    19801 gaaagagctg ctcaaaatga tcagtgaaaa aattgcacag gaaaacaaag tagagccaga
    19861 acatgaagct gaaaaagaca ctgaaaaaga cattagtgga gtaaacgctg caggtaaaga
    19921 atcattaaaa acgaatgatc taaaaacgaa tgatctaaaa acggatatcc cggaaaaaac
    19981 tgaaacattg tctgaaaaac aatcagaaga cgagaaatcg aatgggttgc cggaagcaga
    20041 taaaaaagaa atgctcgaaa aagcagacaa gaaggcaaca gagtcggatg gaataaataa
    20101 tgaagccgat gctgaaactc aatctgcaag tgaagatagc actgctcttg aagctaaaat
    20161 agaaaaagaa gagaaccagc ctgagctgat tgaagcagaa acagaaccaa taacaatcgt
    20221 gccaaatact gaaaaaacgc ctcaagaatc aaacccagaa aaaaaagatt tagataagac
    20281 acaaaagaaa aaatcaaaaa aagtgccgta tctcgcttta gcagcagttc ttctactagg
    20341 tgggggaggc tgggcttatt atgaccatca tcaaaaagta gaagccgaag tagcagcaga
    20401 agcaaaaaga cagcaacaaa tggaagaact tcatgagaaa cttgctgcct tttatacaga
    20461 tgatcaccat caatttattc gtgcagatat gattcatcaa gatttatcaa aactaaaaaa
    20521 tgatttatcc agtcttaaga atgaaaaagg atattcagag ttagaaacaa cttaccaaga
    20581 tatccaatct aagatccgag atatccaaaa agtgaatgag tggttcatag cacctgccat
    20641 cgtcgatgat catctagcag atgacatcaa gttgaaagca gataaagaga tccaagcaat
    20701 cgaaacggat aacacagatt ttggaaaatt gattcaacaa gcacaaacag aagctaaaaa
    20761 tcaatatgct gaattgcaaa cagctaaaga aaaaacagct atagtcttta aagacggaaa
    20821 agtagtggat tcagcaacac gcgaccaata caagacggca aaagaagcag tagaaaaagt
    20881 gaaaaataca gatctatctg ctcagttgaa tgaacaattg caacaagtag ataagatgct
    20941 cactgataaa gagaaaaaag aagaagagga aaagaaaaaa gcagaagaag cgaaaaaagc
    21001 agaaaaagcg aaaaaagcag ctgaagaaca agcaaaacaa gccgctcaag cagcagctgc
    21061 agctcaagcc gctcaacaag ctgcggtcgc acaagcagca cagcaagaag cggcatcctc
    21121 gaccccttcc tcttcaaact cagcgaatca gccaatcaaa tctgccagag caagtgatat
    21181 tgccgatgca tctaatccag catggacgtg ggcaccaggt gtccaaagca aagtgattgc
    21241 tacatgtatc caacgtggat atatcgttga aggaggatac agattagaaa aagccagaat
    21301 cgaaaatggg gaaggctatt acaatctata tgccacttca acaaaatctg cattgatgaa
    21361 aggcattgga gaaagtgctt tgccatttta catcgtaacc atcaactgta aaactggatg
    21421 gttcggagga aatgggagca attaatttta ggcaaatgaa agaggctgtg agcctgccgt
    21481 ttaacaccgt ttattcgaaa aaggggagta tgacacaact gttgtcatac tccttttttc
    21541 acctttccaa accaaggctc agctattcaa gtagataaca ttatgttatg atagcaaaga
    21601 aatgtattaa gaaaaccttt cactatagag ggagaaaaaa atgaacgatt tattagcaac
    21661 tatatttaca ggtattatta ttgatgaaaa tgaaaaaaac tactttgtcc aaaaaaacgg
    21721 acaaaccttt ggcttaagca aatcagaagg tgaacacaaa atcggtgaag cagtggaagg
    21781 attcggctac caaaatcaaa aacatgaaaa tcggatcact acacaaattc ctaaaagccg
    21841 tatcggtcat tacgcttttg gaatcgttac tgctacacga aaagatttag gtgcgtttgt
    21901 cgatatcggt cttccagata aagactttgt cgtatctctt gatgaattgc cgacaatgac
    21961 agagctatgg cctaaaaaag gggatcgctt gatgatttcc cttagagttg atgcaaaaga
    22021 ccgtatttgg ggcagtttag cagatgaaaa aatattcaaa tctttaagca aatatggaac
    22081 agaagaaatg aaaaacaaag atgtgatagg aacagcatac cgcttgaaat taacaggtac
    22141 gtacgtttta acggaagatt tctatcttgg gtttatccat ccttctgaac gatataatga
    22201 acctcgtcta ggcgagcaaa taaaaggtcg agtgattggt gtccgaccag acggtgtact
    22261 gaatctttca ttgaagccaa gagcatatga agctatatca gatgatgcac aaatgatttt
    22321 aaccatttta gaacgttctg cagatcagca aatcaacttt acagataaat cggatccaga
    22381 agaaatcatg aaagcttttg gtatcagcaa aggagcattt aagcgcgcat taggtaatct
    22441 tttgaaacaa ggattgatta cacaagaaaa cggtgtgaca aaactagtca aaaaatctaa
    22501 ttgagaatcc cttgcatgtt tttcttaact agcttataat aattttaatt aacatagtgt
    22561 tatatctttt tttatttgta attattataa attgttgggg ttgagtaaat ggtcatgaat
    22621 gctatttcgg cgatgaaaaa aacaaaacag cagctacatg aagcggggtt taagctgaca
    22681 ccgcaaagag aagctactct gcttgtgctt ttggaaaatg agaaagagca tttatctgca
    22741 gaagaaatct tctttttagt gaaaaaaaag agtccggaaa ttggacttgc tactgtttat
    22801 cgaacccttg aaatcttgac agaattgaaa attgtcgata aagtaagttt taatgatgga
    22861 ttagcccgtt atgatttgag aaaagaaggc gccaaacatt tccatcatca tttactatgc
    22921 ttgaactgcg gcacgatcga ggaagtagaa gaggatcttt tacttgaagt ggagcaagta
    22981 gtagaagaac gttatcattt tttagtaaaa gatcatcggc taacgttcca tggaatctgt
    23041 caggaatgct atgaaaaaga aaaaagaaac cagtccgtct gagtgtctag acggcttttt
    23101 ctttgctcag attatggaaa aagactatca gaatgaagcg gtatctgata tgatagagag
    23161 gcatgagggg gattattatg aaagagcaaa tcaacgatta tctccactac ttgaatattg
    23221 aacggggatt gtccgccaat acacgacaaa gctatgaacg tgatttggaa cagtacttgc
    23281 attttctaca ggagcagaaa attgcttcat ggcaggatgt cgatcgcttt ttagtcatcc
    23341 gttttctgga gaagttgcat gaagaaaaaa aagcgacagc aactgttact cggatgatct
    23401 caagtctcag aagattccat cagtttttac ggcaagagcg tatcactgat catgatccga
    23461 tgcaacatat cgatacgccg aagaaagcac agaaattacc aagtacattg actctgacag
    23521 aagtagaaaa gctgattgaa gcacctgata cgacaaaaac gttagggata agagatcgtg
    23581 caatcttgga agtcatgtat gctacaggga tgcgcgtcag tgaattgatt ggattgaaat
    23641 tgggagatct tcatctttct cttggactgg tacagacagt tggaaaagga gataaagagc
    23701 ggatcattcc tttaggtgat tatgcgatcc aatggctgga acgctactta gacgaagcac
    23761 gtccattact tgtaaaggac gcatctgaaa tgcatgtatt tgtcaataat catgggaaag
    23821 gtctttctcg gcaaggaatc tggaaaaatc tgaagcagct agtacgagag gctgggattt
    23881 acaaagaagt aacaccacat acgctgcggc atagctttgc cacacatctg ctagaaaatg
    23941 gagctgattt gcggacagta caggaattgt taggtcacgc ggatatctct acgactcaga
    24001 tttatacaca tatcacgaag aaaagaatga cggatgttta taagcagcat tttccaaggg
    24061 cttagtcata ggaaggtgag aagatgttaa cacattaccg gaaagaaaac cgtaaaattg
    24121 ctatgggact attgagtttc cataagaaac tgacaggtaa aacttctttg ttgaaggaga
    24181 ttgatacgta cgaatctgaa gacgattatg aacttttttt ctttacgcca gaaggttcaa
    24241 ccaacattca agggattatt ggtgtttact gggcaacgaa cgatcagctg atcatccatg
    24301 acatttcttt gaatccgtct tatcgcggtg agaatatggg ctttcaaatg ttagatgaat
    24361 tacaggaaaa gtacccggat gtctccatcc gtgcaactga aatcacagag ccttacattg
    24421 gaaaatggct agcaaagaaa aagaagaaag agtgaatgag ttgacggaga tcaatattaa
    24481 attagatgta ttcgaaggtc ctctcgatct gctgcttcat ctgattcagc aaatggaaat
    24541 cgatatatat gatataccga tcgctacgat cacggagcaa tatatgaatt atatccatac
    24601 aatgaaaaca ctagagctag ctgttgcagg agaatactta gtgatggctg ctacattgat
    24661 ggcaatcaaa agcaagacat tgcttcctgt ccaagaattc gtcacagatg atgaagaatt
    24721 atgggaagaa gatcctcgag aagaattggt cactcagctt ttagaatacc gtaaatataa
    24781 atatgcagct gaaagactga cagaaaaagc agaggaacga agtctttatt acacaaaaga
    24841 acctatgaac atcgatgggt tagcggatgc tgaaaagccg ttgaagcgag gacaagtcaa
    24901 aaaaatcgat ttgatccgtg ccatgcagaa aatcttagaa cgtaagaaat tttctcaaaa
    24961 agtggaaaaa acgatcacgc ccgatgacac aacgatcgaa gaacgaatgg tatttatcga
    25021 agagcgtata aaaagaaatg cgcatggctg tactttcgat tctttttttg agtcaccttc
    25081 taaaagtgaa gttgtcacta cttttatggc tttgctagaa ttgatgaaaa aagggcagat
    25141 cattgcgcaa caaaaagaaa actatgaatc aattatgctt tatccagcag taggagatac
    25201 cgaacatgac gaaattaagt gaacttgaag cccttctttt tattgttggc gaggaaggga
    25261 tcagtaatga agaattaagc ggactgttaa atctcagcga agcagaagtc acaagtttag
    25321 tgaaagaatt aaaagaatct tacactcaaa actcgcactc agctttgcat atttttgaaa
    25381 cggaaagcca ttatgtatta acgactaaaa aagagctggc tcccttactt aagcgatatg
    25441 cgcaagggac atcaaacaca ttatctcaag ccgctgtcga aaccttagca atcatcgctt
    25501 ataaacagcc aatcacaaga gctgaaatcg agcagattcg aggagttcag gtttcaggag
    25561 cggtacagcg tctagctgcc cataaactga ttgaagaaaa aggtagagta gaagggccag
    25621 gtcggccgat attatatgga acaactggct acttcttaga ttattttggt ttgaaatcat
    25681 tagaagaact gcctgataca caagaaatgg aaatttcatt tgaagaagac gtgtcaccag
    25741 atctattctt tgatggaatg aataaaggag aataattgta atggaaagat tgcagaaagt
    25801 aattgcccac gctggcatca cttctcgtcg aaaagcagaa gaattcatcc ttgcaggacg
    25861 tgtaaaagta aacggacaag ttgtaaggga attagggata aaagttggga aacatgacac
    25921 tgtggaagtg gatgaagtcc caatttatca agaagaatac ggttattatt tattgtacaa
    25981 acctaaagga gttatttctg ctgttgcaga tgacaaagga agaaaagttg ttacggatct
    26041 tttgccgatg gtaaaagaac gtatctatcc tgtaggtcga ctggattacg atacttcggg
    26101 gatcttgcta ctgacaaatg atggagattt tgctcagcgc ctgacacatc caaaacatga
    26161 agtggataaa gtttacgttg caaaggtaaa agggatcgct acaaaatcga tgcttgcacc
    26221 tttgacaaaa ggaataaaga tcgaaggacg aaaaacatct ccagctaaat tccaaatttt
    26281 atcagtggat cctaagacaa accacagtat cgtggaattg acgatccacg aaggacgtaa
    26341 tcatcaagtg aaaaatatgc ttcaagcagt tggtcttcct gtgcaaaaac taaaacgtga
    26401 aaaatacgga gatctgactt tacaaggcct acgtcctgga gattaccgtg aactgaacaa
    26461 aaaagaagtc agcagtttgc tgaaccactc aaaatgaaca cttacttttt ataagtgtta
    26521 gatgttgcgt tttttgaata gatatgtata atgaatatgt aaacaaaaat tatataaggt
    26581 tcgtcttcag gggcagggtg aaattcccga ccggtggtta tagtccacga cctattttgc
    26641 aaaaaatagc tgaatcggtg aaattccgat accgacagta tagtctggat aaagaagata
    26701 gagcttattt gacgacgctt gatcaaataa ctctaactct attttccctg gaaaatagag
    26761 ttttttcatt tggagagaaa cgtcaaaaag ttcgctgaag aggagtccta acaggaggat
    26821 ttcaaatgaa gaacacccgt gtacagaaaa tggttgcagt agcgatgttt gctgcgatcg
    26881 cgttagtttt acagtatttt gcttttccga tcatgccggc ttttagtttt ttgaagatcg
    26941 attttagtga tatcccggta ttgatcagca tgttcttatt cggtccagcg gccggagttg
    27001 caacagcgtt cttgcgttca gtattacatc tgctgactac aggtttttct ccggataatt
    27061 tggtaggaga cactgctagt ttcttatcaa caacgatctt tacattcccg atttattatt
    27121 tctttagtgt aaagaaacag cataaaggaa gaaacaaatt tttaggtgtc attacaggaa
    27181 ccatcgctct aactattttt atgagtatcg caaattattt cgttattacg cctctttact
    27241 taatgttttt aggactgaac gcaacacaga tgctaggcat gtctttagca aactatgtac
    27301 tgattgggat cgttcctttc aacttgatca aaggattcat cgtgagcgct gtctttttag
    27361 tgctgcatgc gcaacttctt ccatggttaa gtcgaaaaca acatcagttc gaacaacgtc
    27421 atacagcagt caaataatta tcaaaaagat caagaaattt cttggtcttt ttttttggaa
    27481 aaaaatacaa gacatgcaat aatgtaaacg ttttatttgt ttgttttgag tatttttttg
    27541 atttatgcat aaaaatcaat caattctttg caaatgtgaa attcttctcc ttttaaaaga
    27601 cgaaaatgtt tgtttataaa aaaaaggaaa cgctacaatg aaaaagaaga aagctttaaa
    27661 aacctgaaaa aagaggaggg aatagggaaa aacgaacttt ttgaacaaaa gttttacaaa
    27721 atgcgtgtta ttatttttgt tctgatactc tttttgataa aataacatta gagccaaaat
    27781 tgttgtctct aacggaaaag aaaggaagaa gaagatggaa caaacaaaaa agaaaaaatt
    27841 tcaaatgcct tcagcgtata cggtcttatt tattattatt gcagtaatcg ctgtacttac
    27901 ttggtttata ccagctgggc actatagtgt agacgatgca ggcaacatcg tcgctggatc
    27961 atatgaacga gtacaacaaa atcctcaagg gctgtgggat attttcatgg cgccaatcaa
    28021 tgggatgtta ggtgtaccag cgggggagct aactaaagaa acacctgctg caatcccaat
    28081 ctctttcttc attttggttg ttggtggatt tttaggtatt atcaacaaaa caggggcact
    28141 agatgccggg attgcttcag ttgtgaagaa attcaaagga aaagaaaaaa tgcttattcc
    28201 agtattgatg ctgttatttg cattaggcgg ctctacttac ggaatggcag aagaaacgat
    28261 tccattctat gctttactga tcccagttat gatggctgta ggctttgata cagtagtagc
    28321 agtagcaatc gtattagtag ggtctcaagt aggctgtcta gcttcaacgg taaatccttt
    28381 tgcaacaggc gtagcttcac aagctgttgg gatttcgatg ggtgaaggta ttggtcttcg
    28441 attcttgatg ttcgttattt tgttagctat ttctattgta tatgtgtatc gttatgcatc
    28501 aaagatcgaa aaagatccaa caaaatcatt agtatacaac caacgtaaag aagatatgaa
    28561 gcatttcgat attgaagcaa tcggtcgtca agaagtaatc acgaaaaaac aaaaacatgt
    28621 taatgtattg ttctttatta cttttggtat catgatcatc agtttgattc cttggacaac
    28681 cttgaatgcg aatttcacga tttttgaatc attgacaaac tggttaacaa accttccggt
    28741 actaggtact gttctaggta aaagcatgct tccattaggt gactggtact tcccagaaat
    28801 tactatgtta ttcttagtaa tgtccatcgt agtagcaatc acttatggca tgaaagagtc
    28861 tgaatttgtc tctgaatttt tagctggtgc ttctgatttg atcggtgtag caatcgtagt
    28921 agctgttgct cgtggtatcc aagtggttat gaacaatgga ttaatcacag acaccatctt
    28981 acattggggc gaacaaggat taagttcatt atcttcaagt gtattcatca ttattacatt
    29041 tattttctat atccctatgt cattcttgat tccttcaact tccggcttag ctgctgcaac
    29101 aatgggaatc atgggaccta tgggtgaatt tgccggcgtc ggtaaggatt tagtaatcac
    29161 tgcttatcaa tctgcatctg gtttagtaaa cttaattaca ccaacatctg ctatcgtaat
    29221 gggtgctgta gcaattgctc gtttcgatgt ttcagtgtgg tggaaattta ccggtaaatt
    29281 aattgcgata ttatttgtag ccatctgtgt gatcctaggt gtagcagcaa tgttctaaac
    29341 ctagacaaat acaggtacaa ggtctatcat aagaatgaag aaaccgataa acggctatcg
    29401 ataagcacgt ggacaagtca atagctccat tcagtgagtg ttttttcttt catcatttgt
    29461 taagaacatt ctgcttgctg atggagcagg cttaaaacgt cattatttga gaagaagagg
    29521 ttgtgtcgga ggaatttgac atgacccttt ttaacatatt aggaggataa tatcatgaaa
    29581 aaatttgtga cagaacaaca tcatgaacaa gcattagaat cattgagcga attgatccga
    29641 attccttccg tactagatga agcagattca ggacaaggac atcccttcgg taaaaaagtg
    29701 attgaagctt tggataaagt attggaaatc agtgaaaagc taggatttag aacatttaaa
    29761 gatccagaag gctattacgg ttattcagag attggctctg gagatgaatt attcggtatc
    29821 ttgtgtcata tggacgttgt tccagcaggg gacgaaaata attgggaaac aaaaccattc
    29881 gatccaacta taaaagacgg ctggttagtt ggtcgcggat cccaagatga caaaggtcct
    29941 tctattgcgg caatgtatgc cgtcaaagct ttgattgatg ctggcgtgga attcaagaca
    30001 cgtattcgtt ttatctttgg tacagatgaa gaaaatttat ggcgttgttt agaaaaatat
    30061 aacgaaaaag aagaaggaat tacgcaagga tttgcgccag atgctgaatt tcctttaatt
    30121 tatgcagaaa aaggcttatt gcaagcttac ttgacaggac caggaacgaa cgagttttct
    30181 gtaaaagctg gaggagcgtt gaatgttgtt cctgatgcag ctccttactc tggagaaaag
    30241 ctttctgaag taaaagaagc gttgaaaaaa cacgaattcg atttcgaaga acaaggtgaa
    30301 ggcatcgtcg ttcttggtaa aagtatccac gcgaaagatg cagcacaagg agtgaatgct
    30361 atttctcgtc tggcaattgc tctttcagaa gtattcgatt ttggcccaat caatttctta
    30421 ggaaaattag ttcaagaaaa tgcaactggt gaagctgtcg tgggcaaaac agaagatgaa
    30481 caatccggcg agctaacgat gaactttgca agtttggaaa tcacaccaga acaaacaaaa
    30541 atcggtgtag atatgagaat ccctgttaca ttcaagaaag atgatttagt agctaaatta
    30601 actaaaacag cagaaaaata tgggttgact tatgaagaat ttgacttcct tgattcatta
    30661 tacgtgccac tagacagtga gttagtgaaa aatctattgg ggacatatcg cgatattact
    30721 ggagacatga ctgaaccgtt tgtatcaggc ggcgctactt ttgctcgtac gatgaaccag
    30781 tgtgtggcat tcggtgcgat gttccctgat actcctgact ttatgcacca ggcgaatgaa
    30841 cgatgggaat taagtagtat gtataaagca atggaaattt atgcagaagc tgtgtatcgt
    30901 ttatgtgcta aataaaaata aacatacaaa aagggcggat attcaaatgg atattccgtc
    30961 tttttttatt tgatgctgga aagtttgcat ttctctctta ttttttttaa ggtataatca
    31021 tacttgatta agaaaaaggg gtcactcaga tagaaaaggt gggagaaagt gaaaatattt
    31081 agacgaatac taattggctt agtcatcatt ttaggattac tcggtggagc cggttattac
    31141 tatatacagc aaaatacgta cgaaccatcg tcagaagcgc aagtgttggt aaaagaagga
    31201 aaaaacaaag aagattactt attttttgaa ggagagaaaa agaatcatgg aaattctcca
    31261 ttacttattt tttatcctgg tgctctcgta gagccagaaa gttatagtat ttgggcgcaa
    31321 gatttagcaa aaaacggctt ttcagtcgct attgtcaaaa tgccacttga tctagcagtg
    31381 atgggcggaa acaaagcgga taaggtcaaa gaagattttc caaattccga ttatgtgatc
    31441 ggtggccatt cattaggtgg cgtaatggca agtcgttatg tcaataagaa tcattcagac
    31501 aaacatttaa aaggggtatt cttcatggca agttatcccg acaaaaaagg aacattgaca
    31561 gaatcagctg ttcctgtttt atcgatcacg gcgacaaaag ataaagtatt gaatcaggaa
    31621 gcttatcaag aagctaagca gtatttaccg gaaagaacag agtatgtatc aatcaatggc
    31681 ggaaatcatg gaggttttgg cagctatggt gagcaaaaag gagatggaga ggcagaaatt
    31741 tctaacgcag accagcaaac tgtgatcgtc aatacattgg aaaactggct gaaaaagttg
    31801 aaataatata aatgggtcct atagggtccg tgaaaaaact agtcaattaa ctatagatag
    31861 caaatgaatc tttcaaaata agtcgggatt tcacggaaca agagaaacag atcccggctt
    31921 attttcaagt ttagagaact tagtgtacgt ttttaaagtt tcgtttatat ttatatttta
    31981 ttttctaata aaatcgctcg aactggacat ttttgatacg ctttttttac atatgtttct
    32041 tcttccggca tcacgaactg ttgatgagcc tctggttctt gggcaaataa aacgattcct
    32101 tcttcatcat aatcgaaaat atctggcgct tcgatttgac aaagaccgca tgcaatacag
    32161 cgttcaggta caagtttaca tagtagtgac atttctaacc atcctaactt aaggagaata
    32221 atatgattgt ttttatttta tcgctatttt catcaggaca caagctgcgg attagttctc
    32281 tttaccaact ccttgtggga aaacgaacga cttcagtact tatttatgga tttacccacg
    32341 aacttttgtt tatccataac agttttccag aattgaagca ggataagttt tatcaaattt
    32401 tgcaaaaaat tgcgcagcaa ggttggatcg aaataaatga aaatgaagca aagctaacgc
    32461 ctgcaggggc tgatatgttg tccgaacatc aaatagaaca tactggtttg cgttttgacc
    32521 gctatggcag aacaggggaa actagttggc ggctcattaa atttgctgtt caagtaattt
    32581 ctaatctggc aacgggtatt caagactatc ttccagcaga gacaagtcct ttttatactt
    32641 tccaactaaa aaaatggctt agtgaaagcc aattgccacg tgaaattttg attgatactg
    32701 cgtatgagag tttggtgcag ctgttttcgg agataccaga agaagcagca gattttttag
    32761 ctaaccaatt atctggaaat gagcgaacag ggctgcttcc ttatcagctg gcaaaaaaca
    32821 atgatgagtc agcggtttat ctgcaacaaa gcagatgtat ccaccttttg ttagcacaaa
    32881 tcgaaaaacg gccagattcg ctttggcacg ctttgatcga ttcattatta cagcaaaatt
    32941 tcaatcaaag catgatgatc accaagcaga tgtttatgaa tggtcagaca attgaccaga
    33001 ttatggcaat ccgtcattta aaaagaggaa cagtaacaga tcatcttatt gagtgggcct
    33061 tgttttttga tgattttcca tacgagcgga ttctctctgc agaaacaatc gaacgtttgg
    33121 aaccaaataa ggattccgtg cgagagtggc gctttagcga atggaatgta gatggacaat
    33181 tagattatgg agaatttcgt ttgtatcaaa tctatctatt gagaaaggaa gcgatacaga
    33241 atgttaataa atgaattaaa aaaatatttt ggatttgatt catttcgtcc cggccaagaa
    33301 gaaatcattc aggcgttgct tgatggagaa gatactctcg ctattttgcc tacaggaacg
    33361 ggaaaaagcc tttgctatca attgacgggg tttttaatgg aaggtctggt catcatcgtg
    33421 tcaccgctgc tttctctaat ggaagatcaa gtgacgcaat tacaaaaacg aggggagaaa
    33481 cgagtagctg ctttcaatag tttactttcg caatctgaaa gaagatacgt cctccgtcat
    33541 ctatcgcaat ataaattttt gtttttgagt cccgaaatgt tgactaatcc ttctctttta
    33601 gagcatctga aaaaacaaaa tattgcgtta tatgtagtag atgaggctca ttgcgtgtct
    33661 caatggggag ttgatttccg tccagaatat caacagttag gaaaaatccg aaagctttta
    33721 ggaaacccgg tcacactggc actaacggcg acagcaacag atttagtagc aagagatata
    33781 cgtcatgtac tatttgatcg accaccaaaa gagatcagac aatcagtcaa tcggcaaaat
    33841 atctcgcttt ttgttcggaa aacgcagcaa aaagaacaag aacttgaaca cttcatggaa
    33901 agagcacaag gagcggcaat catttattgt gcaacaaaaa aagaagtcga acgattgtat
    33961 catttattca gggaacgttt tacagttgga tactatcacg gtggtcttga tgcagctcaa
    34021 agaagacaac ttcaacagca gtttgtaaaa aatcagctgc agtttctcat tgctacgaat
    34081 gcatttggta tggggatcga taaatctgat atccgttatg tgatccatta tgatttgcca
    34141 gacagtctag aaaactatgt acaagaaatc ggtcgagcag gcagagatca ggaagaaagt
    34201 gcagcaattc ttttttatca aagtggggat gaacgtatcc attatttctt taatcaactt
    34261 tctagagaac agcgacaaag ttttgagctt tatttggaat atgccacaga gcaagctccc
    34321 tttgatgagc tgcaaaaaaa atggatggag ctgatccagc agtctgaaaa accagaaaat
    34381 tgggtggaac ggctcaaacg tcaggaaaaa gaaaaagaat tccgcctgca gcagatgctt
    34441 cgctatatta atgaagaaaa ctgccgcaga aaattcatct tagcttattt tggcgaaaaa
    34501 ctctccgaaa agccgcagaa ttgttgtgat attgatggag caaaagcaga acttctgcca
    34561 aagaggaagc ttttttctaa gactgaacaa cttcattggg aaacgatttt actaaattta
    34621 tttaaaaaaa acatggaaca ttgatttttt aggacaagac actttttcct atggtataat
    34681 acacacgata gagtttagga ggtaaaacag tgagcagaaa agatagacat caatctacag
    34741 aaaataacga aacacaagaa ccatgggaac aaccaatcta cgatacagat gatgaagttt
    34801 catcaagaag ttctcagaga caacaaaaga aaggaaatac attatttctt tcgctatttt
    34861 tgattttact ggtcttatgt atcgctattc cagcaggtgc ttatttctgg atcagaaatg
    34921 gatcaagcaa caatgcaaca gcagcaactt cttctgaaac ttcgacatca gtagtagaaa
    34981 gttctacaag tacaacaaaa gaggagtcaa ctgagacttc aacagtagaa tctagtacaa
    35041 tcgcggaaga atctactgtg tctcaagata caggtgtaac gtcagaaagc agtattgcag
    35101 cagagacaac accgacttcc tcaagtgagg caacttatca agcaggggat tcaagcagca
    35161 atgcagcaga aacaacacct tcttcatcag ctgcagccgg ttcaacaacg actgttcaag
    35221 caggcgaagg tccaaaacaa gtggctgagc gtgcagggat caccactgat caattgtttg
    35281 aattgaatgg cttagatcca aacaacttta tgctttaccc tggtcaagaa ttaagaatta
    35341 aataataata gccgcgcttt gcggcttttt ttctgagaaa aaggagttat atcatgacac
    35401 aaatcagtat tgcaatcgac gggcctgctt catctgggaa aagtaccgtt gccaagatat
    35461 tagcaaaaga attgaattat atttatacag ataccggtgc catgtatcgc gcagtaactt
    35521 atttagctat tacgaacaat gtatcttttt cagatgaaga agggctagtt tcgctgatca
    35581 aaaagtatcc tatctctttt tcgcaacaag aagatggaca gcatgtattc atagcatcaa
    35641 aagatgtgac tttagaaatc cgtcaaccag atgtcacaaa agctgtttct gaagtctctg
    35701 cacatggaaa agtcagagaa gaattagttg ctgtccaacg aaaaataggg gaacgcggtg
    35761 gcgttgtcat ggacggaaga gacatcggga cagctgtatt accgaatgca gaagtgaaaa
    35821 tctttttagt agcaagcgta agtgagcgtg cagaaagacg tttcaaagag aaccaagaaa
    35881 aaggaattcc gactgatctt gaaacactga cgaaagagat acaggaaaga gaccattatg
    35941 attcaacaag ggacgtctct ccgctgaagc aagcagagga tgctgttcgt atcgatacga
    36001 ctggcaaatc gattccagaa gtcgttgcag caataaaagc tgttgttttg aaaaaaggtt
    36061 atcttctttt ttaaattaag aaaaaaaatg aaaaacaatt gttttttcaa ggaaaacgaa
    36121 cgaaatcgct ttattttttt tgaataatca attaaactaa aaacagtacc gtacttacgg
    36181 cagaatgttg gttaggagga caagtgttat gacagaattt gatcaaaatc aagagaacaa
    36241 ccagtcaatg gaagacgcaa tgaaaagcgt acaagaagta aaagttggcg atgttgttaa
    36301 gggcgaagtt ttagtcattg aagacaaaca agtgattgtc ggaatcgaag gggctggagt
    36361 tgaaggagtc gttccggcaa aagaattatc aactttgccg gtagaagata tcagcgaagt
    36421 agtaaaagtc ggtgatgtat tagatttagt cgttatcagt acgatcggca aagataaaga
    36481 aaacggcagc tatctacttt caaaacgccg tttagatgct aaaaaagttt gggaagatat
    36541 cgaaagagat ttccaagagg gcaaggtcat cgaagcacct gttacaaatg ttgtcaaagg
    36601 cggtttagtc gttgacgtcg gtgtacgtgg ttttgtccct gcatcaatgg tggaagatca
    36661 ttttgttgca gacttttcag actataaagg caaaacattg agctttaaaa tcatcgaaat
    36721 cgaaccatca gaaaatcgtt tgatcttatc gcataaagcg gtagtggaaa acgaaagaga
    36781 agaaaagaaa aaagaaattc tttcaactat ccatgaggga gatgttctca gtggacgtgt
    36841 tgcacgtctt actgattttg gcgcatttgt cgacctaggc ggaattgacg gtctagtcca
    36901 tgtctcagag atttcacata gtcatgtagc aaaaccaagt gatgtattgg ctgtcaatga
    36961 tgatgtaaat gtcaaagtgt tgtctatcaa tcctgaacaa gaacgtgttt ccttgtcaat
    37021 caaagacaca cagcctggac cttggtcaga aatcgataca aaagctccag ttggcacagt
    37081 attagacgga acagttaaac gtttgacaag cttcggtgcc tttgttgaag tgttccctgg
    37141 agtagaaggg ctagtccata tctctcaaat ttcacataaa catattgcga caccacatga
    37201 agtgcttcat gaaggtgacg aagtgaaagt gaaagtatta gagatccatc cagaagaaca
    37261 tcgtattgca ttgagtatca aagctttaga agaaaaacca gcttcagaag aagaaccaaa
    37321 aaatgttgag tcttatgaat taccagaaga aaatactgga tttactatgg gagacatttt
    37381 aggtgatgcg ttgaaagaag aagtagaaga aaaagcaacc gatgattctg aaaaataatt
    37441 gattttgtat ctgtgactga gatgaaagca gctgcttttg tctcagtttt tcttttgaaa
    37501 agcgattagt atttcaataa ggaataaaaa tacttcgttt aattgacaat ctatgcttga
    37561 aagttggcaa tgcattcgtt aaactaagtg agattggaaa cttgtgagga ggaaataata
    37621 tggcaaatcc aacaattgca atcgttggcc gcccgaacgt cggtaagtct acgattttta
    37681 accgcatcgc tggtgagcgt atctcgattg tcgaagatac tcctggagta actcgtgatc
    37741 gtatttatgc aaaaggtgaa tggttaggtc gagaattcag cgtgatcgac acaggcggaa
    37801 ttgatcttgg cgatgaacct tttatggatc agatcaagca tcaagcagaa atcgcgattg
    37861 aagaagcaga tgtaatcatt tgtgtagtca gcggacgcga aggcgtgaca gacgcagatg
    37921 agatggttgc gaaaatttta tacagaagta ataaaccagt gattttagca gtcaataaag
    37981 tagataatcc agaaatgaga aacgatatct atgaatttta ttccttaggg ctaggcgatc
    38041 cttatcctgt ttcaggaagc catggactag ggattggtga tattttagat gaagcagtga
    38101 agtatttctc tgaagagact gaagaagagg acgacgacac gattaagttt agtttgattg
    38161 gtcgcccaaa tgttggtaaa tcctctttga tcaatgcgat tttaggagaa gaacgtgtaa
    38221 ttgtttcgga tattgaagga acgacccgag acgcaatcga cacccacttc gtttctgaaa
    38281 gtggtcagaa attcttaatg atagatacag ctggtatgcg taaacgcgga aaagtatacg
    38341 aaaatactga aaaatacagc gtgatgcgtg caatgcgtgc cattgaccgt tctgatatcg
    38401 ttttgatggt actgaatgct gaagaaggga ttcgggaaca agacaagcga gtagcgggtt
    38461 acgcccatga agctggtcgt ggaattatta tcgtcgtgaa taaatgggat cttgtaaaga
    38521 aagaaaccaa tacaatgcgt gatttcgaac aagagattcg tgatgaattc cggtatcttg
    38581 attacgctcc aatcgttttt gtttctgcag tgactaaaca acggttggaa cgtctgcctg
    38641 aaatgatcga acaagtaagt atgaaccaga atcttcgtat ctcttcggct gtgttgaatg
    38701 atatcattat ggatgctgta gcaatcaatc caacaccaac agataaagga aaacgcctga
    38761 agatttttta tgctacacaa gtcgctgtca aaccacctac atttgtcgtt tttgtcaatg
    38821 aagaggaatt gatgcatttt tcttatgaac gtttcttaga aaatcaaatc agaaaagcat
    38881 ttacttttga agggacgtcc atccgaatta tccctcgtag gagaaaatag gcattttacc
    38941 atatcgacgt ttttttttca aaagaaaaag acagtttcgt ttgttacgtt tctatgacta
    39001 aaaaaagcat gtgaaaattg gtaaaaacct tgctattaag cggttcatat gatatcgtga
    39061 taacagaata ttgaagttaa tcaatatttc tttttaaact tctggaccac tcagaaagtt
    39121 ttaactaaat gatatttcta tcatatctct tgtggaggag gtgaatctat ccatggcaaa
    39181 caaagcagaa ttaatcgaaa aagttgcttc agctactgat ttaactaaaa aagatgcaac
    39241 tgcagcagta gatgctgtat tttcaactat tcaagatgct ttagctaacg gcgaaaaagt
    39301 tcaattaatc ggttttggta actttgaagt tcgcgaacgc gcagctcgca aaggtcgtaa
    39361 cccacaaact ggtgaagaaa tcgaaattcc agctagcaaa gtacctgcat tcaaaccagg
    39421 taaagcatta aaagacgctg ttaaataaga cagttgaaaa agcgcttgtg gctctagggc
    39481 tacaagtgct tttcttttta tttcctagca gaaaaggaat aaataagaga cagagattcc
    39541 gaatccgtat gtcatacata gaaaacacat gtattttatt taatttttat ttatttgcaa
    39601 gttatgaggt gagctttgct cttatctttt ctagaatttg cttactaatg gtaaaataaa
    39661 aataagaaga ggagggatgc gtatggaact tgattatcaa gtagaagatg cgttgacgaa
    39721 agtgttttac ggagaaacgc tagtcagtca gctaagagaa ctttccattg atcagccaat
    39781 cttattttta acgaatcagc gctattatga tttgtttgca gataagatca acccgttatt
    39841 tgcaaatcaa gcaaacaatg actggtatat ttgtgccaac actcaatgca atcatttaac
    39901 agaattaaag aacctgcttg atttcgtcaa acgctatccc gaaaatcaat ccatgtttat
    39961 catcggattt ggaaatgaag ggatcatgga tctggcaggt ttttttcaaa aacatacaac
    40021 ccttgattca tcactatgga tgatccctgt atcgatccgt tctctaagcc gagcattaac
    40081 accgcagcgg acgattttgc agctaccaaa tcgtgtagtg ctgcaaacca tcaatctgcc
    40141 agaacgcatc gtttatgatc aaacaatcag cgagcggcag gtggacggaa agcagattga
    40201 ctttttgact tttatctgtt gcggaatgat ttgtgatcat cgttttttgc aaaatctgta
    40261 taaaaactat ccaaataaaa agcaattaac gactcggccg tttgccggga tgatcgaaga
    40321 aatgatctat ttttatcaat tagaggcaga aaacctgtca aattatggta aactgtttga
    40381 acaagctttt tatctgaccg aaaatggcca tctactttct gctagtatga aaaaattttt
    40441 aggaatgatg tttcatttcg tgtggaatct ccaagcatat ccttttgatt tccaactaaa
    40501 aaactttttt gtgtggctgg acttgcttgg ctatccaatc cactggctgg aacagatttc
    40561 aaagatggag tatttggaaa aagttctttt tttggcagaa aaaagcaaaa aaatacttgt
    40621 tttagaaaaa ataggtataa tagacggata ccaacatcca aacgaaaaag aattattgag
    40681 aacaatgacg gtatttcaac aaatcgtcag tgagataaga gggaattgaa atgaatacaa
    40741 atagcgaaaa aatgttgcaa gctttatcag atgaagactt gtcacaagca caaatctttt
    40801 tagaaaaagc attaaaagaa gacccagcag atatattagc tgaactaggc gaagaattgc
    40861 tggctattgg ctttttgatc gaagcaaaac agatttttga acaactgatc aaccaatacc
    40921 cagaaaatga cggactgaac atcccgttag cagaaattgc aatcgaagat aatgagatcg
    40981 acttagcttt tgaatacctg gaaaaagtgg ataagcaaag tgattactat ccgcaaagct
    41041 tattggcgat agctgatttg taccaagtta tcggaatccc tgaagtgagt gaagcaaaat
    41101 tgaaagaagc agctgggttg cttcctgatg aaccattgat ccagtttgcg ctagctgaat
    41161 tgtatttttc agttgatcgt ttcaaagagg cagcagtggt ttatgaaaca ttgttggcaa
    41221 gcgggataaa tgaaatttca ggaatctcga tgcaggaacg tttaggacaa tccttaagta
    41281 tgcaaggaga attcgaagca gctatcgtac ctttagaaga agcactgaaa gaagaaagga
    41341 cagatgatcg actgttccag ctagctttta cgaatcttca attgaaagaa aatcagcagg
    41401 caatcaacta tctggaagaa cttcgagaag ttaatccgca ttatcaatcg ttatatctat
    41461 atctaggaca agctcttcaa gaagaagaaa tgattgaaga agcacaaaca gtgttagaag
    41521 aaggaatcaa agaaaatcct taccaagtcg aactttatca cctggcctcg gaaaatgctt
    41581 tccgtctgca tgatagacag cgagcagaag aacttttact gaaagcttta gaagtaggag
    41641 acaaacaaga tgaaacatta cttactttaa gtaatcttta cttggatgat gaaagatatg
    41701 aagatgtgat acaagtgatc aatcagatgg aagaaactgc taatccttat gcagaatgga
    41761 acctagccca tgcttataat gaattagaag actttaccgt tgcagctgta cactatgaac
    41821 aggcttatca tgaactatca catgaaccag atttcttgaa agagtatgcc tttttcttaa
    41881 gagaagaagg gcagctgaaa agagcgcaag aattattgac ttattattta acacttgaac
    41941 caggagatat ggaggcactg tcattattag atgacctaac agagaggtga gaaaaatgct
    42001 aattgatgta cgggaaaaga agaatttttt aacgtggatg gtcaatcatg tatcttttag
    42061 ccgaagagaa gttttttgga tcttgaatta tcttgccaat catgaagcga ttttaagcaa
    42121 tgtccacttt gttgaagggg cgaattttgc ggatcgagga ctgcagatca gtgatctatc
    42181 tgttgaaggg gaacccatga aattgttttt gcagggaaaa gaatttacgg acacagacca
    42241 gatttttcat gaaatccgtt tgaattggaa aaagccttta tatgtggaat gtatattcga
    42301 aaatgcttgg cagacaagag aatatctatc tatcttagaa gacaatccat tttcttcttg
    42361 ggattcttca gtcagtgaag aagatactga attaattgaa cgatattttc aagaaaaaga
    42421 agaagaggca aaactaaaaa tgctttatgc tcaaatcgat caagcactag aagatggaga
    42481 tagggatgct tttttgaaat tatctgatga agttaaccga aagatccttt tgcagacaca
    42541 aaagaaaagt aaccgggtaa gtgaacataa caattagtta aaaatagtat agtactaaaa
    42601 aagacaaagg tcagctggat gtggaatctt tcctcatagc ctatctttgt cttttttctt
    42661 atagtttttt cagtaatctt gtctgtggct tcaaataagt aaagccttgc ccttccactt
    42721 catgcacatt gatgattgaa aggaaagctt tttcgtcaat ctcgtggacg atcctcttag
    42781 cttcagttaa ttcactagag ctaacgacca tatagatgat tttttttgaa ttaccagaga
    42841 atgcgccttc tccagaaagg aaagtcacac ctctttgcag accggtcatc agtagtggtg
    42901 caatctcttc gcttttgttg gagacgacaa gtagaccttt tgcagaatat ccgccatcta
    42961 agatcgaatc aatcacacga ctgaaaacat acgaaacaat caacgtatac atcatacgtt
    43021 tcaagtcgat ataggttaat gaagcaagta agacgatgat atcaaatgcc aatagggaac
    43081 gccccatagt gatgccgtaa ttttgttcta aaatccgagc gataatatca ctgccgcctg
    43141 ttgttccacc catgcggtag acgatcccgc ttcctacacc tgcaactaaa cccgcaagta
    43201 gagaaacgat cagtagatca tgctgtaaat tgatttctag tggaaccctt tgccagatcc
    43261 ataaaaagaa tgacaaagaa agtgtaccta gaacagtata ggccaaggaa cgtttaccga
    43321 ggatctttcc tcctagtaaa atcaatggga tgttgattaa taaagtcgta taagctggat
    43381 caataccaaa taaagctctt aagatcaagg tgatccctga gactccccct tctgctaaat
    43441 gattagcgat gttcaagtaa acaagaccaa atgcataaat acttgttcct ataatgataa
    43501 aaagaacttc ttttactgtt tttcgtttga ccatatgaat tccagctttc aattatgtat
    43561 tttcacagaa tttccattta gaaattcctt cctaaatgta gcatggaaat tcgattttct
    43621 caattgctct tatctaggat ttgcgttaaa gttttggtaa gatagttttg gagggaaaaa
    43681 gatggataaa aagcgttctt tatacagtat gcagcaggaa gtggatgaat atatccagca
    43741 attcaaggtg gggtatttct ctccattagc tcagatggct cgattaactg aagaagtagg
    43801 tgaattggca agagaagtca accacactta tggagaaaaa agcaaaaagg cttctgaacc
    43861 agttaactcg gtagcagaag aacttggtga tgttttattc gttacgatga tcatggctaa
    43921 ttcattgaat atcgacttaa ctgaagtttt tgaaaaaaac atggaaaaat tcaatcaaag
    43981 agaccataat cgctttgagc gtaaggacga gtaaacaaga tgaagtggtc ctggccttga
    44041 agactctgaa acggacttgt caaacagtca ccatgcaact gagtctttat ctcaggacaa
    44101 gaaacacgca ggaaaggttg gaaaaatgaa attaaaacaa ttacctctgg aataccaaaa
    44161 ggcaattcca gttctgaaaa aattagagaa tgcaggatat gaagcttatt ttgtaggtgg
    44221 aagtgttcgt gatatcttgc tgaaccagcc aatccatgat gtggatatcg caacaagcgc
    44281 atttccagaa gaaatcaaac agctttttcc aaaaacgata gatattggga tcgaacatgg
    44341 aactgtgttg gtattagaaa atgatgaaca atatgagatc accacatttc ggacggaatc
    44401 aacttatcag gactaccgta gaccagatca tgtcgaattc gttcgttcgt tggcagaaga
    44461 tttgaaaaga agagatttta cgatcaatgc gtttgctttg aaagaagatg gagaaatcat
    44521 cgatttgttc gatggtctgc aagatctaga agaacagatc ttgcgagctg taggcaatcc
    44581 tcatgagcgg ttccatgaag atgccttacg aatgatgcgc gggttgcgtt ttgtcagtca
    44641 actagggttc aaattagagg aagaaacctt ctacgccatc aaagaaaacc acgagctatt
    44701 aggtaaaata tctgtagaac gtataaatgt ggaattcatc aagatgctct tgggaaatga
    44761 acgggccgcg ggaatccatg catttattga aacagagtgc tacatttgct gtcccggttt
    44821 gaaagaagaa ggcgaagcac ttttacgttt tgctgaatta ccatccgtcc cattgaaaga
    44881 agagagccaa gcatgggcat tattgatcag ccaactggat ctaaaagaaa atgagattcg
    44941 ttcctttttg aaatcgtgga agtgttctaa ccaaatgatt aaagacgttc aagcacttgt
    45001 ccaaggacta cgacaacgag aaactggagc tttggaacca ttcgaattgt acttcttagg
    45061 caaaaaacat gcactgcaag tagaagaatt gatgatctat tttgacgaaa cacctcagat
    45121 ggatgccgta ggagaatcct atgatcgttt acctatcaag tcattaagcg aattagcagt
    45181 agatggtaaa atcttgctag aagaaactgg aaaaaaacct ggtaagtggg ttagtgaagc
    45241 tttgcaatta gctgaaaaag ccgtcgtaga acaaagaata aaaaatgata aaaaacaggt
    45301 tttggatttt ttatcgaaag aaaaattgat ttaaaaaaca ctagactgta tctgctcagc
    45361 tttgttggct acttaaagtg ggcagatacg tcattagaag aaaaaacata agatgtgacc
    45421 acttacataa atttgtgcta aactgaggta gtacaagaat aaattacatc ataaaggagt
    45481 ctttttaatg agtaaagaaa tgacagcgct aaaattttat tttagaaatg gagaaacctg
    45541 gacgatcaac cgccgtcata tcggcgatct ttggatcaaa caaatcacaa caagttttgg
    45601 acgaatcaat ggcagtgaat ttgttgaaat ccatccgtgt gctggattta aaatcgaaat
    45661 cttccatgaa ggggacgcag ttgcgacaca tgatatcaac ctaggcggac tggaaatggg
    45721 catgtttaat cgcgcattga aatacgaaga tatcgagcga atggaaatcc tttaccgaaa
    45781 tggcacacct gatcttgtgt acttcccata tctagataaa ggaacagaag gattagacaa
    45841 tcaatatcaa tcaacaaaaa tcagcgaaaa aacaggtaac ttgtatatcg tgatcaatcc
    45901 agatcaacgt gtagaagatg tatacgggga atttttcgaa taaatcgatt tttacgcaaa
    45961 aatgaggctg tgaaagagat gtgtcttatt actactgata ggctgtctct tccacaccgt
    46021 ttattcagaa taagggactg tgacaagatt tttgtcgcag ccccattttt ttgttgtgat
    46081 aaaagaacaa gaaaggcagg aatgggaaag taaacatcct tttgtcagaa acttttatct
    46141 ttcttaagtt ttacttgata tctgatacta tttggaatag aatgaggcgt tgaaaaattt
    46201 atggaaattt ctaactagca ataaacgatg aaacaatgaa atatttttca tccaaaaaga
    46261 actttgctat aatgatggaa gtttgtgtat aggagatctg gaaaatgaga gagagttttg
    46321 aacaacaaaa aaaactgttg catgatcgct atggcgccct ttcgatggat gaccgccgtc
    46381 aaatactttg taagttgcgt aaaagaaata tattgatgta tcgtcagttg gaacgtctga
    46441 aacatgatct gcttcgtttg gagtcaaaac gtgtacagtg tgaattagag gggaaccaaa
    46501 cacaggttga agtagtcgaa accaaaattt taaagaaaaa agaacaattt ttaaaaatgc
    46561 ttacacaaaa taaaaaataa aggggatgga cgatggaatt tgctgagtta gttattgtat
    46621 ttgcagtaac gatcacctta tcgaacatag ctagccgcat tttgccaatg attccagctc
    46681 cactgatcca aattttcctt ggagtggtat taggtctgac agagtgggga caatcaatcg
    46741 attttgaacc tgaattgttc ttagttatga ttattgcacc attgttattt agagaaggag
    46801 aaaaagcaga tatttcttcg attctcaaaa atttcgggac aattttgttc ttggcatttg
    46861 gaggagtcat tttgaccctt gtcggggtag gtgcgacgtt atcattcctg ttgccaagtg
    46921 tcccacttgc tgcctgcttt gcttttggag cagcgttagg tccgacagat gcggtagcag
    46981 tcagttctct ttctggacga gtgaatatcc ctaaaaaggc gatgcatatt ttggaaggag
    47041 aaggtctttt gaatgatgca tctggtgtta ctgccttcca gtttgctctt ggtgcgctga
    47101 tcaccggatc tttttcagca gtaaacgcgg ggatgagtct agtagtttct agtattgggg
    47161 gagctttgat cggctttttg cttgtctggt tcaaacaaaa aataattcat ttgatcgaaa
    47221 aagcatcagc acaagatgtc acagggtatc tgctgatcga acttttgctt ccttttctag
    47281 cctatgtatt ggcagaggca tttgatgtat ctggaatcat tgctgcagtg gcagcaggta
    47341 ttctccaagc ttctggtttt aggaaaatct ctgtgtttga tgcagaattg tcgagcttgt
    47401 cccacagtac atggacaact attgctttta cactgaatgc attagtattt atatttttag
    47461 gaatcgaatt gacgcaagtg ttctcgccag tatggggaga cggtttatat ccaaacggtc
    47521 ttttattagc gattatcgta ttaatctctg tcatgctttt tgtgatcaga ttcatttcta
    47581 tcagtctttt ttatgtattc aaagatggca gtaaaaagtt caaaaaacag ttgaacgaga
    47641 tcttgatctt aacatttggc ggtgtaaagg gaacagtcag tttggctacg atttttattt
    47701 tgccattttc aatcaataat atgatgtttt accagcggtc tttgcttttg tttctgacgg
    47761 cgggtgtcat cttagtgact ttggtcatag ggatcattgt cttgcctatg ctgacggaaa
    47821 cagaggaagc gaaaagcacg gatttgaatg ctttgatgat tttagaagag gtcgttgaag
    47881 ttttgcgaaa agaaataaag gaaattgatc gtaatacaaa agaattttta gccactgaag
    47941 cagtgataga aaattatcaa gaacggatac gtgatctgta cttggaagat ttaaccgatg
    48001 atgaaaagca agaagtacaa gaaatacaag cactgatttt gtcgatcgaa cgggatggat
    48061 tggatgaaag ttatcgttcg ggcaagcttt cttctaatgg ttatcgtttt tactcccgtt
    48121 ttctttctcg attcgaacat tctatcacaa gccagatttt gtcttttatc ggcttttggt
    48181 tcattgttgt ccgtaggtta atgcgaatcg ttctccatcc gaaaatgttt tggcaaagaa
    48241 gatatgctga tcgtcagaca tttattagtg atgaagatat ccaagaaatc agagagacat
    48301 atcaaaaaaa tactcagttg attattgaaa gtttagataa cttgactgat atttatgatg
    48361 aaacgatggt tcaatttttt atccagcaac gcaaacttga aggaataaaa atgaactcgg
    48421 ggaatttgat ttcttcgtgg atgatccagc aagatagtct gtttacgaaa aaaatgctaa
    48481 gaggttatta tcttgaaaga aaaatcattg atgaatacga agttgctgaa aaaatcacga
    48541 cgttttctgc gaacagctat cgcaggaata tcaatttgtt agaatcttat acgatgaaca
    48601 aaccatctga taacttttct ttccgatttg cgtttcgagc aaagtcaaaa aaaacgagtc
    48661 caataaaata aaaaggtaaa aattttcaag ttttataaaa aacaaaacat atcagaacag
    48721 gatcgaatcg gtcctgttct ttttttgaga acacaaaaag aacaaatacc aaaaaaattt
    48781 tcctgaagtt cactattttt atgatacaat atccaagata aggagggaac atgttgaaag
    48841 aattaaaagt aactgacctc aaaaaaactt atggtgaaaa agatttgttt gaccagattt
    48901 cttttttggt ccacgaaaaa gaccgtatcg ggttgattgg gacgaacggg acgggtaaaa
    48961 caagtctctt aaatattatt gccggtatcg atagcagcga cggtgatcgg caaacagtgt
    49021 tttatccaac tgattaccgt attggttatc tttctcaagc agctgaattt tccgatgaat
    49081 taactgttct ccaagcagtc tttcaaggaa acagcccctt gatccaaact gttcgagcat
    49141 atgaggaagc attgatcgaa ctaggggaaa acggagaaga ccctgatgtg caaaaacgtt
    49201 atgctaaagc agaagaacaa atgaataaag aagatgcttg gactacagat accaacgcaa
    49261 agattatttt gcaaaaatta ggaatcagtg aattagataa aaaaatcagt actttatcag
    49321 gtggtcagaa aaaacgagtt agtctggcgc aagtgttgat tgatgaaccc gatttactat
    49381 tactagacga accaacaaac catttagatt atgaagcgat cgagtggttg gaaaactatt
    49441 tgaaacaata ccgtggcgca ctattgatgg ttacccacga tcgttatttt ctagatagag
    49501 tagcaaaccg aatcttcgaa ctttcatttg gaaaattata cgagtataaa ggaaattatg
    49561 aagcttacgt cttagaaaaa gcagaacgtg atcgtgtaga agtagaacaa gaagaaaagc
    49621 gcaagcggct ttataaacag gaattagctt ggatgagggc tggcgtacag gcacgtggca
    49681 ccaagcagca agcacggatc aaccgtttcg aagatctaaa ggagaacttg taccaagtaa
    49741 accaagaaga tgatttagag ctgaacctag cgacacaacg tttagggaaa aaagtactgg
    49801 aaatcaaaga tggcagttac cgtataaatg accaaacact gctagaacat ttagatttat
    49861 tgattcaatc tcgggaaaga ctaggtatta ctggtaaaaa tggtgcggga aaatcgacat
    49921 tgcttaatat tctagctggc agaattcccc tggatagcgg tacaatgtct atcggtgaaa
    49981 cggttcatct tgcgtattat acacaagaaa atgaagaaat ggcaccagat aaacggatga
    50041 ttgcttactt acaagaagct gctgaagaag caaaaacagc agatggcagt caaatcggag
    50101 tagcagaatt acttgaacga tttttatttc caagatttat gcatgggaca ctgataggca
    50161 aactttcagg cggagaaaaa cgccggttat ttttgttgaa actgctgatt cagcaaccta
    50221 atgttttatt attggatgaa ccgaccaatg atttggacat agcaacattg acgattttgg
    50281 aagattattt tagaagtttt ccaggtgctg tgatcactgt ttcccatgac cgttatttct
    50341 tggacaaggt agcagataag ttattagtat tccaaggaaa cggaaaacaa gaactttatt
    50401 acggaaacat gtccagttat ttactgaagc aaaaagagac ccagcagcct gctgaaaaag
    50461 cgaaaccaaa aatacagagc aaggaacctg ctgaaaagaa gaaattatct tacatggaac
    50521 aaaaagaatg ggaaacgatt gaagatgaga ttgcagaact tgaagaaaaa atctcattgc
    50581 tccaagaaga gatgaatcat caaggagata atttcactcg attacaagaa ttgcaaaacg
    50641 acgtatctga aacagaagca caacttgaag aaaaaatggc acggtgggaa tacttgagtg
    50701 aatgggtaga agattaggag gaaaaaggat ggaacaagcg tatcttgact taggaagaaa
    50761 attgttggaa gaagggcatc gtaaagaaga cagaacaggc acaggcacat taagcttatt
    50821 cggataccaa atgcggtttg atctgcaaaa agggtttcct ttgttaacaa cgaaacgagt
    50881 accttttgga ttgatcaaaa gcgaattgct ttggttttta aaaggagata cgaatatccg
    50941 ctatctatta caaaataaaa accatatttg ggatgaatgg gcatttgaac gctacatcaa
    51001 aagtccagac tataatggac cagatatgac tgattttggc catagatgtt tgacagatcc
    51061 tgaattcaat gaagaatata aaaagcagag caagcttttt tgcgagaaga tcctaaccga
    51121 tgattccttt gctgaaacat acggcgatct tggacacatc tacggctatc aatggcgtca
    51181 ctgggaaaca aaagacggtg gctttatcga tcaaatcaaa caagtgatcg aagcaatcaa
    51241 aaagactcct gattctagaa gattgatcgt ttctgcatgg aatccggaag atgttcctag
    51301 catggctttg cctccttgtc atacgatgtt tcaattttat gttcaagagg gacggctaag
    51361 ctgccaattg tatcaacgca gtggggatgt gtttctaggc gttccgttca acattgcaag
    51421 ttatgcgttg ctgactcacc taattgcaca tgaaacaggt ttagcggttg gcgaatttgt
    51481 tcacacatta ggagatgctc atctttatat caatcatatc gaacaaatga aagaacaatt
    51541 gagccgagaa atacgcgcat ttccgacttt agttttgaat caggaaaagc agtcagtatt
    51601 tgattttgag atggaagata tccaattaga aggatatgac ccacatccgg cgatcaaagc
    51661 tcctatcgct gtttagaatc agaaaggatg agaaaatgtt agctgcaatt tgggcacagg
    51721 atgaaaacgg attgattgga aaagaagacc gtcttccttg gcgtttgcca aatgatctga
    51781 aattcttcaa acaaatgaca gaagcaaata cactagtgat gggacgaaaa acattcgaag
    51841 ggatgggtag ccgtccgttg ccgaatcgtc agacgattgt tttgacgaga gattcttcct
    51901 ataaagcaga aggcgtgcat gtcatgcata gtgtggaaga cgtgcttgct ctggaaaaag
    51961 aaacagacgg tatctttttc atcgctggag ggtcagctgt ttatcaagaa tttctgccat
    52021 tttgtacgat attataccgt acagtcatcc atcatgtatt cgatggagat gcttattttc
    52081 cacctgttga ctggtctgat tggtcactga tcaatactag tcaaggagaa atggatgaaa
    52141 aaaatcgtta tccgcatcaa tttgaaacgt atcagcgtaa tgagaatcct gtaaaaagcg
    52201 ggacaggcga attgacgata taaaaaagct atggatagac tagctgttat ttagagctgt
    52261 cccatccata gctttatttt ttttattaag taaagaataa aacagagaag tacataaaac
    52321 ctgcacctaa cataacaaac aaatgccaaa caacatgcat atagcggata gaattcaagc
    52381 tataaaagaa tgcgcctatc gtgtaagaaa gcccgcctgc taccatcaaa ataacaccag
    52441 tcattccaag agagtgataa agaggaacag cagccacgac acagagccac cccataaaaa
    52501 tatagatgat cgtagatact tttgatactc tctcccgttt atgcaacgtc aacgctttat
    52561 atataatgcc aagaatcgca aacaaccaga ttaatacaaa taaagtccaa cctagccatc
    52621 ctcggatact cagcaaacaa aaaggagtat aacttcctgc aatcaaaaga aaaatcgaac
    52681 tatgatcgaa tacttggaac accttttttg cttttgtaaa aatcaatgaa tgaaacaaag
    52741 tagacgttaa aaataaaaga atcatcatcg atccatagat agcataagag acaatatgga
    52801 gggaggaatg caagtgggca ccttttacta aaagaaccac tagtcccgcg atactcaatc
    52861 cagtcccaat tccatgggtg actgcattaa acacttcatt taatatttca tacgttcgat
    52921 taaatttcgt tttttccatg aaaacggctc ctttactagg tgtaattaat gaaaaatcat
    52981 tatttgtctg ttatactaaa aatagaaatt gtcttacatg tagtttaaca tgaataaggc
    53041 atcttgttaa agaaagagag agagaaaata atgacaaatg taaaaatcgt aacagattct
    53101 tcatgtacga tggaaaaaaa tgtacgcgat gctttaggca ttcgtgtcat gccgctatcc
    53161 atcatgatcg acggaacagt atatcccgat gatgaccaac tagacggcga aagatttatg
    53221 gaaatgatgg cgaaagcaaa aaatctgccc aaaacaagcc aaccaccaat tggagaattc
    53281 atctcgatgt atgatgagct aggagaagac ggcagtgaag tgatttcgat ccatatgaca
    53341 aaaggcttga gtggaacagt agaagcagca agacaagcca gtaatctaac aaaaacgaaa
    53401 gtaacggtta tcgacagtga taccactgac caaggattat cttttcaggt aattcgagca
    53461 gctgaattag caaaagaagg taaaacagta gagaaaatcc ttgaagaagt caaaaaagta
    53521 caagacaata caaaattata catcggaata tccacactag ataacttggt aaaaggtgga
    53581 agaatcagcc gaacaactgg tttgctgtct agtgttttga acatacgcgt ggtgatgaat
    53641 ttcgcccatt ctgaattaat ccctgtaact aaggggcgag gaaagaaaac gttcgataaa
    53701 tggtttaata atttgaaaaa tgaactgaaa gaattgccaa atgtgcgaca aatcggtatt
    53761 tcacatgcgg atgcaaaaga attggcgcaa gaattcaaag agggtttgca agaattattc
    53821 ccggacatgc atatccctgt actgcatacg aatccggtta tcgctacgca tactggtaaa
    53881 gacgcgtttg caatcatgta ttatgtagat tagtcgaaaa gaggataagc cctcagcctg
    53941 aaaggcttgc agcttattct ctttttcttt ataaaattag gaaaataatt gttttctttt
    54001 ttcataaaga ctaccttttt gataaaattt ttgataacat tagaaccaat cgaatacaag
    54061 aggtgaaaaa atgaaattaa cgaaacgaca tttgatcctc gttttgttcc cattattaat
    54121 tgcgatcatc atctatgcag gtttagctgc ttctataccg aaagctggcc cactaatgaa
    54181 accagtaact actgtgagag aagatccaaa taaaaaagaa aaacttcact acatagctat
    54241 cggagattcg ctgactgaag gagtaggaga tcaaaccaag caaggaggat tcgttcccct
    54301 tgtcgcaaat gatctaaaag accgttataa tttgaccgcg gttgaaacag aaaactatgg
    54361 tgtaaatggt gaacgaagtg accagatctt aaagcgagta aaaacaaaag aagaaattcg
    54421 aaacaatatc aaaacagctg attttatcac actgactgtt ggcggtaatg atttgatgaa
    54481 agttatccaa aataatttat tcgggttaac ggtcaattcc tttaaaaagc cactgaaaaa
    54541 ctatcaggaa aatgtcacag aactattgga agaatttcga tccctcaata aaaccgcacc
    54601 tatttacatt ttaggcatct acaatccatt ttatttgaac tttcctgaga tcactgacat
    54661 gcagacaatt gttaacaatt ggaacaaagg aacagaaacg gtgacaaaaa aaatgccgaa
    54721 ctgctacttt atcccaatca atgatttgct ttatcaagga ttgaatcagg aagtaggtgt
    54781 cacagaagaa ggaacagatc aaacaactag cacgacagaa aatctaaatg gcatcaaaaa
    54841 taatgtgctc tatgaagaag atcatttcca tccaaataat ttaggttatc aattgatggc
    54901 aaatgcagtg agagatcagc tgatcgagac acaaaaccaa tggttagaga aggaggatga
    54961 tcaataaaca tgaaaacaag atctgacgta tcttcaaaca aaacaaagaa aacaaattac
    55021 tggaaattcg ccttccttat ccttcttggg gtagtacttg gttcgaccgt atttttaggc
    55081 agccgtattt ttgctaatcg ggaaccagaa ctcccagaaa taccagctct tacggaaaga
    55141 caaggagatc ctgtcttaac gatcaattca aataaagaaa aagtcaatca aatcatcagc
    55201 tttttcctta gtgagtatca aaaagatagt gatattgaat ataaatttta tttggaaaat
    55261 gaagcactac tcaatggtac tttcgaagta ctaggttttc ctatcgattt ctatttatat
    55321 tttgatccgt atgtgatgga aaacggaaat gtccaactaa aagctagaag tctttctatc
    55381 ggaacattga gtttgccaat caaagatgtg atgaatatga tcaagagaaa ctataaatta
    55441 ccagattgga tcgaagtcaa tacagatgat ctgactatca tgcttaggtt ggatcaattc
    55501 cgaatgcaaa acgggatgta tattaaagca gataaaatcg acttagtaaa tgatgacatt
    55561 cggtttagtc tatatttgcc aaaagatacc tctgatgctt caaacaactc aaataccaac
    55621 aaaactagca ccagcagtac tgaaagcaag acaaaagaaa ataaaaaatc atcggatgag
    55681 tagtattaga gggggaatca taatggaacg agcaatattt gcaggaggct gcttctggtg
    55741 tatgatccag cctttcgaca cgcttcctgg tatccataca gtgatgtcag gatatactgg
    55801 agggcatgta ccagatccga cttatgaaca agtaaaaagc caaacgactg gacatacaga
    55861 agcagtggag attatctatg atccaacatt gatttcatat gaacaattag tagaacttta
    55921 ctggcagcaa actgatccaa ctgacgcttt cggacaattc gaggatcgcg gtgacaatta
    55981 tcgcccggtg atcttttatt tgacagaaga acagcggatc attgctgaaa aaagtaagaa
    56041 acagttagct gaaagtggac gtttcgaaca gccaatcgtt acaaaaatcg aaccagccca
    56101 accattttat ctagcagaag aagaccatca agagtattat aaaaagcatc ctgaaaattt
    56161 taaaagaaac catgcacgcc gggctgcctt tatcgaagaa aattggagga acgaagggtg
    56221 aatcgaagct tttttcatta tttaatgaca ctgcgaggcg gtggcacaca tgatgcttta
    56281 tctgttttcg ctacagaagc cggtaaagac atccaatttc ctaagcacag ctctagctac
    56341 gaagaaattt ctaattatct ggaactgaat gtcgactatt tgccgtccat ggatatcttt
    56401 gacgaagcat gggaaaaata tttagaaaat aatcatgaca tataagagat agttttgtac
    56461 tttatgatca aaaaagtttt ttgtccaata gcctaattga aagaattatt tttatttcag
    56521 ctatctagtc gcattttttt gatatttttc ttataataaa gaaagaatga aaaaaagagg
    56581 gatggattga tagagattga caaaaaaaca acgctatatt aatcgcttct ggcttgtatt
    56641 caaatactta cttgtttctg tattccttgc gttcatgttg cggggatttt tattcatccc
    56701 ggtaccagtg gaaggcaatt caatggaaaa cgtgttgaag caaggtgata tggttgtgat
    56761 ggaaaaattt tcggagatca gacgatttga tattgttgtt tttcaattag cagatggcac
    56821 gatttatatc aaacgagtaa tcggtcttcc aggagaaaat gtcagctacc aaaatgatca
    56881 attgaagatc aatgggaaag tagttaagga accttatcta actaaaaacc tgaaatcaga
    56941 tcatgcaaat gcatcctata cgacggactt tacactacaa gagctaacgg gacaatccaa
    57001 gcttccggaa gataattatt ttgtacttgg agataatcga agagtttcaa aagatagtcg
    57061 ttcgtttggc acgatcaata aaactgatat tctaggtaaa gcaagatttg tctattatcc
    57121 acttgatgaa ataaaatgga tccagtgaga aacaataaag cgtgcagtct gttgagactg
    57181 cacgctttat tgtttaatcc aaaatcagtt tactacctta gtacgtaaaa tcaaacagct
    57241 cgtttaaaca cttttctagc ctctaaagtt ttgataaatg aataaacaac cattatttga
    57301 atagggattt ggatcatatt cctgatgaat ctacctagaa aaattacttt gaaatctgca
    57361 ccaaacaaaa cagacatcag ccaagaattg ataaaaaggc tgactgggat cataatcaat
    57421 aattgggcag cgatgacgga aggcaggttt atctttttat gatataaaaa taagccatta
    57481 ataaatccac caaggaaagc cgtgataaga aaagcagctt gaaacgttcc tgaaccaaat
    57541 atcgtgaaat ttaaagtgtt cgacaacatt tcaagcaaac cagcccagat cggtccatat
    57601 agataaccca taagcgccat acttaaaaat ccgaatgtga tttgaaaata agcaggacca
    57661 atcgctatct tgtataagat gacatcaagg gctaataaaa gagcagaggt cgacaacaat
    57721 cttgcattca tttcgggcaa tctaaaaacg tcttttgcaa tcaaagtgtt caaaatattc
    57781 tctcctttgt cagttaataa taaaaggata ccataaaatc accaatgtgt catgacacat
    57841 tggtgattta catacactgg catctaacaa ttaaaaaaag ggtgagaaga ctgcttttat
    57901 aaactgtaaa aaagagattg tacgaataaa aaaaatagag gcggagaata cttatcgtat
    57961 catccgcctt tgtttttttg ttataaaaaa cgtcagatta gttatctgct tacatcttca
    58021 tccatacggg ttctaatgta tttccgtcta gatcttggac ttctaatgaa aacatttctt
    58081 cttcaggcat tcccatatct acttcgtgga aagtaccacc attttctttt gcaatttgag
    58141 caaatttttt tacttcatcc gcattttcaa cactaaacga aatcaaagca ccacttgttt
    58201 ttttattatc agctatttct ttatctttta taaatttttt ataaaaatca tgagagagaa
    58261 gcattatcca aaaagtgtca tcccatacca ttgaaccagc ttcttcagta gaaaattctt
    58321 catttttttt aaaacccagc ttttcataaa aagcaacgga acgattgaca tcagcaacag
    58381 gaaaattaac aaaaaccata ctattcattt gtttcgtctc ctttctgttt tgttcgtcta
    58441 tcatctctta tttaggaaac gctgtcaaaa atttagattc atatatagaa accaaaataa
    58501 aaatttgcat catcaaaaaa gtgaagatgt gatagaatga ggactggttc actttcactt
    58561 aactaataga tggaatttct ttataacgcc gattgtaaaa ttaagctaga caactaaaaa
    58621 agtcatttgt gctacactca aaatagttcc gtccaaagaa ttataaggag tgagtacaaa
    58681 tgacctatac ccatcttaca acggatgaac ttgtaatgat agagtcttat ttcaaaataa
    58741 atcaatctgt tgcgaaaact gcgcatttct tgaatcgttc aagacaaacg atccataaag
    58801 tatacctgtt cttcaagcaa ggaaaatcag ccttagagta ctatcaacag tataagaaaa
    58861 acaaatcaaa ctgtggtaga cgcccgcttg ttttacctga ggaacaatca gaatatattc
    58921 aaagaaaggt tgttcaagga tggacacccg atgtgattgt tggtcgtgca gcgtttccta
    58981 ttcgttgttc tgctcgtacc atttatcgta tgttcaaaaa ggggctattt aatccttctg
    59041 acttaccgat gaaaggcaag cgtaaaccaa atggacatca agaaagacgt ggaaaacaag
    59101 ctttccgtcg ctctattcat gaacgtgaaa aagattatag ccaattctca aatgagtttg
    59161 gtcaccttga aggtgacact atcgtaggtt tgaaacataa aagtgctgta attaccttag
    59221 ttgagcgatt atcaaaagtt atcatcacat tgaaaccgtg tggtagacaa gcgattgata
    59281 ttgaaaacaa attaaatcat tggtttgaat ctgtaccgaa aaatctattc aaatccatca
    59341 cttttgattg tggaaaggaa ttttcaaatt ggaaacagat cagtaatg
//
LOCUS       DAXLPI010000003        55368 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXLPI010000003 DAXLPI010000000
VERSION     DAXLPI010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.003"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..382
                     /locus_tag="ACF3T5_000126"
     CDS             <1..382
                     /locus_tag="ACF3T5_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3630770.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
     gene            452..1510
                     /locus_tag="ACF3T5_000127"
     CDS             452..1510
                     /locus_tag="ACF3T5_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630771.1"
                     /translation="MNVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTF
                     YPFFQLLRGEEINRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKL
                     SVFSIISLAELIFSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLD
                     MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRA
                     MIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLV
                     ILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQ
                     FGLTVLGICTFIITVINR"
     gene            complement(1750..1833)
                     /locus_tag="ACF3T5_000128"
     tRNA            complement(1750..1833)
                     /locus_tag="ACF3T5_000128"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1797..1799),aa:Leu,seq:gag)
     gene            1906..2067
                     /locus_tag="ACF3T5_000129"
     CDS             1906..2067
                     /locus_tag="ACF3T5_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630772.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            2216..3433
                     /locus_tag="ACF3T5_000130"
     CDS             2216..3433
                     /locus_tag="ACF3T5_000130"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFD3630773.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            3617..4453
                     /locus_tag="ACF3T5_000131"
     CDS             3617..4453
                     /locus_tag="ACF3T5_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3630774.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            4529..5677
                     /gene="nagA"
                     /locus_tag="ACF3T5_000132"
     CDS             4529..5677
                     /gene="nagA"
                     /locus_tag="ACF3T5_000132"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFD3630775.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            5955..6956
                     /locus_tag="ACF3T5_000133"
     CDS             5955..6956
                     /locus_tag="ACF3T5_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFD3630776.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
     gene            6975..7994
                     /locus_tag="ACF3T5_000134"
     CDS             6975..7994
                     /locus_tag="ACF3T5_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFD3630777.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(8096..8812)
                     /locus_tag="ACF3T5_000135"
     CDS             complement(8096..8812)
                     /locus_tag="ACF3T5_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD3630778.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(8781..9566)
                     /locus_tag="ACF3T5_000136"
     CDS             complement(8781..9566)
                     /locus_tag="ACF3T5_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD3630779.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(9557..10306)
                     /locus_tag="ACF3T5_000137"
     CDS             complement(9557..10306)
                     /locus_tag="ACF3T5_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFD3630780.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            10604..11128
                     /locus_tag="ACF3T5_000138"
     CDS             10604..11128
                     /locus_tag="ACF3T5_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFD3630781.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            11144..12643
                     /locus_tag="ACF3T5_000139"
     CDS             11144..12643
                     /locus_tag="ACF3T5_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFD3630782.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(12731..13333)
                     /locus_tag="ACF3T5_000140"
     CDS             complement(12731..13333)
                     /locus_tag="ACF3T5_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFD3630783.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            13437..14393
                     /locus_tag="ACF3T5_000141"
     CDS             13437..14393
                     /locus_tag="ACF3T5_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFD3630784.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(14698..15654)
                     /locus_tag="ACF3T5_000142"
     CDS             complement(14698..15654)
                     /locus_tag="ACF3T5_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HFD3630785.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITINKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            15828..16388
                     /locus_tag="ACF3T5_000143"
     CDS             15828..16388
                     /locus_tag="ACF3T5_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630786.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            16381..17205
                     /locus_tag="ACF3T5_000144"
     CDS             16381..17205
                     /locus_tag="ACF3T5_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFD3630787.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            17215..17832
                     /locus_tag="ACF3T5_000145"
     CDS             17215..17832
                     /locus_tag="ACF3T5_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFD3630788.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            17850..18533
                     /locus_tag="ACF3T5_000146"
     CDS             17850..18533
                     /locus_tag="ACF3T5_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFD3630789.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            19216..20499
                     /locus_tag="ACF3T5_000147"
     CDS             19216..20499
                     /locus_tag="ACF3T5_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3630790.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            20515..21402
                     /locus_tag="ACF3T5_000148"
     CDS             20515..21402
                     /locus_tag="ACF3T5_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3630791.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            21419..22255
                     /locus_tag="ACF3T5_000149"
     CDS             21419..22255
                     /locus_tag="ACF3T5_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3630792.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            22258..22881
                     /locus_tag="ACF3T5_000150"
     CDS             22258..22881
                     /locus_tag="ACF3T5_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630793.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            22878..24170
                     /locus_tag="ACF3T5_000151"
     CDS             22878..24170
                     /locus_tag="ACF3T5_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFD3630794.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGNVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      24529..24626
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            24742..25323
                     /locus_tag="ACF3T5_000152"
     CDS             24742..25323
                     /locus_tag="ACF3T5_000152"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFD3630795.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            25326..26633
                     /locus_tag="ACF3T5_000153"
     CDS             25326..26633
                     /locus_tag="ACF3T5_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFD3630796.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            27049..27537
                     /gene="purE"
                     /locus_tag="ACF3T5_000154"
     CDS             27049..27537
                     /gene="purE"
                     /locus_tag="ACF3T5_000154"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFD3630797.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            27530..28654
                     /gene="purK"
                     /locus_tag="ACF3T5_000155"
     CDS             27530..28654
                     /gene="purK"
                     /locus_tag="ACF3T5_000155"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFD3630798.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            28667..29962
                     /gene="purB"
                     /locus_tag="ACF3T5_000156"
     CDS             28667..29962
                     /gene="purB"
                     /locus_tag="ACF3T5_000156"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFD3630799.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(30142..30519)
                     /locus_tag="ACF3T5_000157"
     CDS             complement(30142..30519)
                     /locus_tag="ACF3T5_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630800.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            30775..31503
                     /gene="purC"
                     /locus_tag="ACF3T5_000158"
     CDS             30775..31503
                     /gene="purC"
                     /locus_tag="ACF3T5_000158"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFD3630801.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            31503..31757
                     /gene="purS"
                     /locus_tag="ACF3T5_000159"
     CDS             31503..31757
                     /gene="purS"
                     /locus_tag="ACF3T5_000159"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFD3630802.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            31759..32430
                     /gene="purQ"
                     /locus_tag="ACF3T5_000160"
     CDS             31759..32430
                     /gene="purQ"
                     /locus_tag="ACF3T5_000160"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFD3630803.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            32431..34653
                     /gene="purL"
                     /locus_tag="ACF3T5_000161"
     CDS             32431..34653
                     /gene="purL"
                     /locus_tag="ACF3T5_000161"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFD3630804.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            34638..36077
                     /gene="purF"
                     /locus_tag="ACF3T5_000162"
     CDS             34638..36077
                     /gene="purF"
                     /locus_tag="ACF3T5_000162"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFD3630805.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETNFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            36100..37152
                     /gene="purM"
                     /locus_tag="ACF3T5_000163"
     CDS             36100..37152
                     /gene="purM"
                     /locus_tag="ACF3T5_000163"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFD3630806.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            37149..37727
                     /gene="purN"
                     /locus_tag="ACF3T5_000164"
     CDS             37149..37727
                     /gene="purN"
                     /locus_tag="ACF3T5_000164"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFD3630807.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            37724..39259
                     /gene="purH"
                     /locus_tag="ACF3T5_000165"
     CDS             37724..39259
                     /gene="purH"
                     /locus_tag="ACF3T5_000165"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFD3630808.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            39275..40549
                     /gene="purD"
                     /locus_tag="ACF3T5_000166"
     CDS             39275..40549
                     /gene="purD"
                     /locus_tag="ACF3T5_000166"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFD3630809.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            40671..41585
                     /locus_tag="ACF3T5_000167"
     CDS             40671..41585
                     /locus_tag="ACF3T5_000167"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFD3630810.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(41718..43283)
                     /locus_tag="ACF3T5_000168"
     CDS             complement(41718..43283)
                     /locus_tag="ACF3T5_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3630811.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(43276..44727)
                     /locus_tag="ACF3T5_000169"
     CDS             complement(43276..44727)
                     /locus_tag="ACF3T5_000169"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD3630812.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(44741..45757)
                     /locus_tag="ACF3T5_000170"
     CDS             complement(44741..45757)
                     /locus_tag="ACF3T5_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3630813.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            45977..47065
                     /gene="chvE"
                     /locus_tag="ACF3T5_000171"
     CDS             45977..47065
                     /gene="chvE"
                     /locus_tag="ACF3T5_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFD3630814.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            47119..48645
                     /gene="mmsA"
                     /locus_tag="ACF3T5_000172"
     CDS             47119..48645
                     /gene="mmsA"
                     /locus_tag="ACF3T5_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFD3630815.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            48647..49852
                     /gene="mmsB"
                     /locus_tag="ACF3T5_000173"
     CDS             48647..49852
                     /gene="mmsB"
                     /locus_tag="ACF3T5_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFD3630816.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            50109..51086
                     /gene="guaC"
                     /locus_tag="ACF3T5_000174"
     CDS             50109..51086
                     /gene="guaC"
                     /locus_tag="ACF3T5_000174"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFD3630817.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            51350..52774
                     /locus_tag="ACF3T5_000175"
     CDS             51350..52774
                     /locus_tag="ACF3T5_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFD3630818.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(52816..53073)
                     /locus_tag="ACF3T5_000176"
     CDS             complement(52816..53073)
                     /locus_tag="ACF3T5_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630819.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            53235..54344
                     /gene="pdhA"
                     /locus_tag="ACF3T5_000177"
     CDS             53235..54344
                     /gene="pdhA"
                     /locus_tag="ACF3T5_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFD3630820.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            54347..55324
                     /locus_tag="ACF3T5_000178"
     CDS             54347..55324
                     /locus_tag="ACF3T5_000178"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFD3630821.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        17731 a         9455 c        12065 g        16117 t
ORIGIN      
        1 aaaagaaatc tgtgatgact ttaaggctgt ttatcaagct aactcaaaag aagaagcgaa
       61 taccttctta tccggcatga ttgagaaatg gaagaaaaac tatcctaaag tgacgcagtc
      121 actcatagaa aaccaagact tattaacttt ttatgatttt ccacctagca ttcgtagaac
      181 catttactca accaatctaa tcgagtcttt caataagcaa attaaaagat acagccgtag
      241 aaaagagcag tttcaaaatg aagaatcact agaacgcttt ctagtcagca tttttgatac
      301 atacaatcaa aaatttctaa acagaagcca taaaggtttt caacaggtaa ccgatacatt
      361 agtttcaatg tttactgagt aactaattat tttgcaggag gacaatttat ttacacaaaa
      421 ttattgacgc tcccagtatg tttttgagaa aataaatgtt aaaaatttat tattggtttg
      481 ctctatatac tgtttaatac cattgacagt aatgggggaa accggttgga gagcaaccac
      541 cttgaactat cagtggccag tagcatttag tttactaact ttttatccgt tttttcaact
      601 cttaagagga gaagaaataa atagaaaaat atattgggta agtattcctt tattgatatt
      661 tttaactaat caagaacaag tcaacgcctg tttttttgtt ctaacaagta tagttagttt
      721 atatttgatt gtcaatggac gatataatta taagttatca gtattttcaa taataagctt
      781 agcagaatta attttctctt tgacaactcc agggaatgca ttacgtgctg cacatgaaat
      841 taataaatgg tttcctgaat ataaaaattt caactttctt aataagttag atttaggtat
      901 ttcatcattt ggaaagccgt tttttttaga catgaatatc ttatttttat tgctattttt
      961 cttaattttc cttctaacat ataggaaatg tcaaaactat tatgtgcgta tattgactgc
     1021 attaccgttt ttcttaaacc tgattattta ttttggtaat acaatgggtc aaagctttac
     1081 ttacgtcaat ggaaataaaa gggcaatgat ttggagtagt agtaacctga ataatctctt
     1141 tacagagtta ggaacgaaac tgtcactttt ttatccaggt acttggatag ctactttagt
     1201 agtcttggca ttattactat gtttaattgt tggaatttat ttaagttttg acaataagaa
     1261 aacttctatt tttttagtta ttttgatgat aatgggattt tgttcaaggt taattatggg
     1321 attttctccc acagtatggg cttccggaat gcggacgtac tatatacttt atgttgttat
     1381 cgccatctta gtactaatgg cagttaaaga gctaatgaaa agcatgagtg tacaaaaaaa
     1441 tgaatttatg caatttggat taaccgtgct tggtatttgt acctttataa taacggtgat
     1501 aaacagataa tatacaaaaa atccctcgct ttagtattag gtgagggatt ttactcgttt
     1561 ttcgattaat tgttttaaca cttgcaagtt tctacgagta gcttgtttcc gaccttcata
     1621 tactcacctc aggctgttta catgattgta cgttcttttt gtaaaggata cagtgatatg
     1681 tatataatgg cataaagaaa cgttcaaaca aaaaaactcg tgaagcaaaa ttaacttcac
     1741 gagttgtagt gcagatgatg ggactcgaac ccacacgagc taacgctcac atgaccctca
     1801 atcatgcttg tctgccaatt ccaacacatc tgcgtaataa taacacacta atgatagcga
     1861 ggttttacat agttgtcaag ctcaaaaatg ggaggactta ttaagatgga gaaatatgac
     1921 ggagaatttt caggactggg aatgatctta ggtgtattga ttggacttgc attcggacgt
     1981 tttttgcttg gttttatgct aggaatcatt tgtggaattg caatggattg ggcagcgaat
     2041 ctatggaatg actatcatga caattaagtt ctcaacgctt gtttgtgttt ttttaatact
     2101 tacgtttttc ctaaataagt ggcaaaattt tctaggatgg agtatgatat tgaaatagta
     2161 aataacaaaa aatttggaaa gttctaactt tttgaaatga gggattggct aagtgatgag
     2221 agaatttgaa aaatccaaca aattagaagg tgtcagttac gatgttcgcg gtcccgttct
     2281 agaagaagcg gagcggatgc aagaagaggg cattagtatt ttaaaattga acacgggaaa
     2341 tcccgcacct tttggatttg aagcaccaaa cgaagtcatt cgtgatttga tcatgaatgc
     2401 ccgtgactca gaaggctact cagattccaa ggggatcttt tctgctagaa aagcaattga
     2461 gcaatattgc caactgaaac attttccgaa tgtgacgatc aatgatatct acaccggcaa
     2521 cggagtgagc gaattgatca cgatgtgtat gcaggggttg ctggacaatg gagatgaagt
     2581 actggttcca atgccggact atccgttgtg gacggcttct attgcattag ctggaggcac
     2641 acctgttcat tatatttgcg atgaagaggc agaatggtat cctgatattg atgatatcaa
     2701 atcaaaaata acgagtcgga caaaagctat tgttatcatt aatcctaata atcctactgg
     2761 ggcactttat ccaaaagaat tattagaaga aatcgttgaa gtagctcgac aaaataacct
     2821 gattatttat tccgacgaaa tctatgatcg cttagtgatg gatggtctag aacatattcc
     2881 gattgctaca ttagcgcctg atttgttcgt tgttacgctg aacggtctat ccaaatctca
     2941 tcgtgtagca ggatttcgag taggctggat ggtcctaagt ggagataagt cacacgtcaa
     3001 aggctatata gaagggctga atatgttgtc ctccatgcgt ttgtgttcca atgtattgtc
     3061 tcagcaaatt atccaaaccg cgctaggcgg ttatcaaagt gtcgatgagc tgctcctgcc
     3121 aggcggaagg atctatgaac aaagagaata tatctataat gcaatcaatg atattcctgg
     3181 tctttcagcc gtaaaaccaa aagctgcttt ttatatattc ccaaaaattg atacaaagcg
     3241 attcaatatt ttggatgatg aaaaattcgt tttggacttt ttacatgaac atcatattct
     3301 acttgtacat ggtggtggat ttaattggaa ccagccagat cattttcgaa tcgtttatct
     3361 tcctaaaatg gatgatttga aacaaacagc taagaaaatg agagaattcc tagcaaccta
     3421 tcgccaaaaa tgattttaaa aaccaaattt ttcagtagga gggccacaga caaacacact
     3481 gaggctctct ttctagtgga gttttcttaa ataactaaag tatattattt agttgcaagt
     3541 cttttcttga agtttgcgac ttcctaaagt aaaatacgaa tagaatgcga atacgcatat
     3601 tttggggagg atacttatgg aaacgttaat cgagtccttt ggaacgatca ttaaagagat
     3661 tcgaaaagaa caaaaaatga cgcaaaaaat gctttcgcat gatatttgtt cacaaagtgt
     3721 attaagtcgg atcgaaaata atgaggaact gccgaatgta ttggtgatga tgcagatttg
     3781 tcaacggcta ggggtaacga ttgatcatgt gatgaatctc tctcgaaaaa ctactcggac
     3841 aaacgataaa ttatttgaac tgttggatag ttgtttccaa aaacgtgatt atcggaagtt
     3901 agaacaagta ctaaaatctt ctgatatttc tgaaaatctg tatcaagcaa ctgactttca
     3961 acgctattat tattatctcg gtgtttgcga attcacgttg cgtcaaaata tctctcagtc
     4021 cctttattat ttgaaagaag cattaagcta ctctagccaa aaagaaagaa tccatgtttc
     4081 tgacacagaa atacaactga ttagttgtat cggaaaaata tatggtgtgg caggaaaaac
     4141 ggctgaagca cgctactatt tgagtcgaag catccaactg ctgcatcaat cagctgatga
     4201 acgatcaaaa agtgaatttt cgcaaatttt ttataattat ggaaatttct tattccatca
     4261 aaatgaaatc gacgaagcgt tagaacaggt gaatcaagga atctattggg ctcaagagaa
     4321 gaatagctac tattacctca atgatttatt tgtgcttaaa agcttaatat acaagcgtaa
     4381 agaagaacca gaaagagcag cattctatga aggcttagca caagctgtga aacaaatttc
     4441 aaaaaatttg taaatggtgt agaccattct tgacttcttc tctaaataag agtactttag
     4501 aggtagaaaa acaaagggag agaatagaat gagaactttt atctatgctg acaaattctt
     4561 tttagcgtca ggtgtcaaac atgcaggcta tcttgaaatt accgatggac tttttggtga
     4621 atacaccaaa gaactacctg agggagaagt caacatcatt gaccaaagcg gcaaatggat
     4681 tgctcctggt ttggtagata cacatattca cggctatatg aatcatgatg taatggataa
     4741 tgatgcagaa ggcatcaaag tgatgtctga aggtctgtta tcttgtggag tgacttcatt
     4801 tttaccaaca acattgacgt caagcaaaga acgtttaaaa gatgtagctg agacaatcgg
     4861 caaaatgtat caggaagcac caggagctaa aatcaaagga atctattttg aaggtccttt
     4921 cttcacagaa gaacacaaag gggcacaaaa cccaagttat ttcggggatc ctgatttgga
     4981 tactttccat gaatggcaag aagcttcagg tggtttaatc aaaaaaattg ctttagcacc
     5041 ggaacgtcaa ggtgtagaag agtttgtcaa acaagtgact gaagaaggag tagttgtttc
     5101 tctaggacac agtaatgcta cactagaaga agcgcaaaaa gctgttgaag caggcgcaag
     5161 tgtatttgtc catgctttca atggtatgcg tggattgaat catcgcgaac caggtatggt
     5221 gggtgcgtta ttatctttag aacatgtctt ttctgaattg atttgtgatg gccatcatgt
     5281 acatccaaat gctgcgggaa tcctaatgga aaaagcagga catgatcatg tcgcattaat
     5341 tacagactgt atgatggccg gtgggatgcc tgatggaaat tacaatttag gcgaatttcc
     5401 agtagtagta aaagaaggaa cagcacgtct agaatctggc aacttagctg gaagtatatt
     5461 gaaattgaaa gaagcaatta aaaatgttgt agactgggga ttagcaacac cagaacaagc
     5521 agtcatgatg gctagtctga ttccagcaat cagctgtaaa atcgatgata catgtggaaa
     5581 aatcgctcaa ggacgcgatg cagactttat cgtattgaac cctgatatga cattggaagc
     5641 aacttatcta gatggcgtgg aacgtttcca cgcataatat aatttaagtt aaccttaaaa
     5701 ctaaacttct taagtttggt tttaaggttt tttttgtgtt ctagtaaaaa tcactttaca
     5761 taagtttcaa aagtttcttt aatttcctat tgttcaataa gtaacaatgt aaagataaaa
     5821 ttaaaaatgt taatacgtat tagcaaaata aaagcttttt ttattgtgaa taaatgaaca
     5881 taattaggag gagaaagatg aacttatttg acatttttcg atcgactttg acagatatgt
     5941 cgattgtcag tgcaatcact tcaacagttt ttattatatt attagggttt gtttgtagaa
     6001 aaaaggggat tttttctgaa tctgttggaa aaattctatc gaaagtcgtc ttaacagtgg
     6061 ctttgccagc actggcattt cgagctttca tgcaagatat caatacagaa ttactgatgc
     6121 aaggaatcaa tgtcttgatt tggggattag ttatttatat tattcttatt ctagtttcaa
     6181 aacctttttt cttaagctat aaaggggata agcaaacaac gttgcgtgtc ctgacgatct
     6241 ttggttctac gacctttttc ggaacgccta tcgtcagtgc gatttatggt cctgtcggcg
     6301 tgatgtattc ctcaattttc aatattggct accgaatctt tttgtattct tatggataca
     6361 taaaaatgag tggcttgaag atggaagcta aaaatatcaa agcgatgttc gtcaatccaa
     6421 tcattatcgc tacttttgcc ggccttttca tttggctgtt ccaagggtat ctgccacaag
     6481 tgtcagttac aggtgcagaa ggagagataa gccaagtagc gtttttgcga attgaccaga
     6541 ctgcaccgtg gctatttcag ccgatggatt atttagctgg tttaagttca ccattggcgt
     6601 ggctagcaat cggcgcaaca ttaggtgaag taagcttcaa aaaagctgca tctgaaaaaa
     6661 cttcatggta ttacagtcta gtcaaagtgg tatttgtacc tgcattgaat atcgtgattc
     6721 tagcagtttt gacggcaacg aatattctac cagtcaattt tgaagcactg gcaacaatcg
     6781 ttatcatgat ggctacacca gctgcaacag tggcagcagc ctatgctatc agttttgata
     6841 gggaggcact tttagcctcc aatgcatcct tactgtcaac tatttttgct gtggtactga
     6901 cacctgtatg gatcgttgta ttacaaatga ttcatggcat aggtttattt tcataagaaa
     6961 tggaggaaga aagtatgaga atcgcatgtt acggtgttcg tccgaacgag atagattttt
     7021 ttgaaaaatt gaatatttac cattatgatc tcagtcttta tgaagaactg ttgacacatg
     7081 acaatatcgt gtcagcaaag gaccatgatg ctgtattgtt gaggggaaat tgtatagcag
     7141 atgagaccaa tcttgcaaaa atgcaggaat atgggattcg ttatgttttt acacggactg
     7201 tgggagtcaa tcatatcgat ctacaagcgg cagctgattt tggtatgaca gtagcccgtg
     7261 taccatcgta ttcgccaaat gcgatagcag agttatcttt gacttttgcc atgatgcttt
     7321 tgagaaatac tgcgtatacg acgatccgaa catctttcaa agattttcga gtggatgagc
     7381 aaatgtttag tcgtgaaatc agaaattgca cagtagggat cataggaaca ggcaggatag
     7441 ggttgacgga agctgttttg tttaagggac taggagccaa agtgatcggc tacgatattc
     7501 atcagacaga agctgctaaa aaagtattga cgttccactc gcttgaagag ttattagcca
     7561 aaagcgatat tgtcagtatt catgtacctc atatcccagg tgaaaacgat caaatgatca
     7621 atgaaggatt tttagctcaa atgaaaaaag gtagcatctt gattaataca gcccgtggag
     7681 agcttcaaga taatgaagct attttaagag ctttgaaaag caatcattta gctggttttg
     7741 caaccgatgt ttttgcaaat gaaacagagg tttttttccg atccttcaag ccatgggaga
     7801 cgattccaga ccctgctatc cagcaattag tagaaatgta ccctagagtt cttgtaaccc
     7861 cgcatgtagg ttcaaataca gatgaggcat tatcaaatat gatttcgaca agtttcgaga
     7921 actttcgtga aataatagaa actggaaaaa cgaaaaatga ggtatctctt cctaaagccc
     7981 gtcaattaag ataagaaaac atggatattc gtcaataaaa tgtttaaaaa tagtccaagt
     8041 aaacagttgt tacttctgta tacttggact attttgtatg ggataattcg atgactcaac
     8101 agtaatctgt gagctttttc cgcccattct ttgtcaattc ataacaggtt agcggccggc
     8161 cgatttccaa atatttgatt ctttcgttca atatatcttg ttctactaaa aaattcaaat
     8221 atttcttgac ggatatacgt gacagtgagc ttttttgagc cagttctttt gttgaaaaaa
     8281 tagtatcttc ttttagaata aatgcactta ttttttttaa agtcagtttt gacagacctt
     8341 taggcaaatc gatgaatgat ggtatttcgt gaatgggagt ggtgtggttg aatacttgat
     8401 cgatcaattt ctgatcggct ttttcagtcg tttggattgt ctgagagaaa gcaagatact
     8461 tttcgatcgc cagctgaaaa cgttgcaaag tgaacggttt gatcaaataa tctaaggcac
     8521 cgtagcggat cacattcttt atggtttcga tatcatctgc agcagtgatc aaaatcactg
     8581 ggatatcatt tccttgctgt ctaagccaag ataaaaaatg gataccactt tcgccaggca
     8641 tgtatacatc aagcagcagc agatctgcgt ttttttgtct gaaatatttt tttccttctt
     8701 ctacatttct gaaactttcg attacgttca tattagggat gctttccaca aaagtacgat
     8761 tcaattctgc aaccattgga tcatcttcga ttatcaggac atttgtcatc attttgatct
     8821 tccttttttg gtaattctag atagacgatt gtaccgccat ttgtttgttt ggaaaattct
     8881 aaaaatccac cgtggcgttt gacgattgaa tggatgagat tcaatccaaa cccacgattg
     8941 attcccttcg tcgaaaaacc tagcgtaaat attttttctg agatttcttc tgatatcccg
     9001 acaccgtcat cgcagacggt cacgatgaat acttcagctt ctgtgtcata gtttaaatga
     9061 atccggacct gtccgtttct ttcatgtttt tgtagcacgg cttcttttgc attatcaagt
     9121 aagttcccta acacttgcaa aagtgaatgg atggagtcat ccatctccat tttagggata
     9181 aaagaatcgc tttctaacag catttcaaca gattgttctt tggcttcgtt tattttgcct
     9241 aataaaaaac cggcaattgc aggtactttg atcagacttg agacttggcc aatctctttt
     9301 ttataatttt gatttagctg ctggataaaa tgggtgactt cttcatactt cttcaaatca
     9361 atcaaaccag aaatgacatg aagtttattc atgaattcat gggtttgagc tctcatggag
     9421 tttatatatt gtttcgtccc cccaagctcc tccattaatt tcattacttc tgatcgattt
     9481 cgtaaagttg cgactgctcc taaaaaacgg tcattgctaa aaagaggtgt aacgctagaa
     9541 atagtttcta tcccatttaa atacatggat tgagtccgaa cggtcgtttg atgctcaatc
     9601 gtctctttaa acaaaacgtc atatagatta tcactgattt ttttcctact tcgaaatcca
     9661 atagctgctc tgtttggtag aaaatgtttt ttgcaggtgt attgatcaag aggatagttt
     9721 tatcagaaga gatggctaag attccttcat ctacttcatt ttcgataatc gttttttcac
     9781 ttaactgttc tgctatttct tgtggttcaa gtccaagcag tacttttttc attttttggg
     9841 caagaaaaac ggcgcctatg atgcctacgc taagtccaat aatcaatgaa aaaacaatca
     9901 ttttttgtcc atctttgact tgattgtcaa tcgtgttgtt tgttaagcca acacaaactg
     9961 caccaataat ctcttgatca taataaatag ggacgaaata gcggaagccg aaccctaaca
    10021 ttccttcttt ttcagagaaa aattcttgtc ctccaatcac ttttttcaca tcttcatgac
    10081 ttgagaaagg aagaccgatg taacttgggt ctgcgtgaga atatcgaatg ccttcagggt
    10141 ccatgacaac gataaaatct aaattcgatg caatcatgat ttcatttgtt attccttgaa
    10201 tcgcatgtgt cgggtcacca gtctgcagct ttttttggat gagtttatca ttggcaacga
    10261 ttttagcaat gctagagatt ttatctgaca cactagatcg gttcatcgaa agaataaaat
    10321 tcctgatcaa tatggcagaa acaatcaaag aaacgaatgt ggagaaacaa acgacaagga
    10381 tgatcgtgtt ctggagcttt aatggttttt tcttaaaaaa aggaaacata ggctacctcg
    10441 aaagaattta ttgtgaaaat tttaacatga aaacaaaaat ttaacaaatt gtcaacgata
    10501 aacacaacct tcctataaaa aacaccactg agtcggtaaa agaagctata cagaattttc
    10561 aaacattggt atgatagacg tagaaatgga aggaggcgtg tatatgatag ggggaatcgc
    10621 acttgagacg atttattggt atacattgct gatttgtgca ggattggcag tcctattgat
    10681 tatttttgga gatgtattcg attttgatgg cccgcttgat ccgatgctta ttatcccttg
    10741 gttaactttc acaagcttat ttggttttct aggtgagcgt ttaacagatc aatcatcttt
    10801 atttttattt attgctagtg gaggcatatc gatagtgttg gtatttttgc ttaatttcta
    10861 tatattgatg ccgatgaaac atgccgaatc aacgatgtcg tcttctgaaa aaactctgga
    10921 aggacaagta gctgttgtga tcacaccgat ccctgtacga ggtatgggag aaattcagtt
    10981 gaaaagtgtt accggttcca ttagtcgtcc agcttgtttt tacagccccc aagaacagac
    11041 agctgagcgg ggaacgaaag tattgatcat tgagatcaag gaaagaatct gttatgtcat
    11101 gccttatgaa agcatgataa aagcttaaag gagcgtgtgt taaatggaaa taccagttgt
    11161 ttttatcgcg atcgcaatcg cagctttgat tttactgatg ttgctgatcg tctttgtagc
    11221 gaagtaccag acagcaaaac cggatgaagc gttgatcatc agtggaagtt atttaggaag
    11281 caaaaatgtg cacattgatg aaagcaataa caaaatcaaa attgtgcgtg gaggtggcgc
    11341 attcgtcttg ccagtttttc aacgttcgaa tcggatcagt ctattatcga gtaaattgga
    11401 cgtctccacc cctgaagtgt atacggaaca aggcgtaccg gtgatgtgtg atggtacttc
    11461 gatcatcaaa atcggttcat ctgtagaaga gattgctaca gcagcagaac agtttttagg
    11521 caagacaaga gaagaactgg aaaatgaagc aagagaagta ttagaaggac acttgcgctc
    11581 gattcttggt tctatgactg tagaagaaat ctatcaaaat cgcgataagt tcagtcaaag
    11641 cgtacaagaa gtcgcttctg tagaccttgc aaaaatgggg ttgatcatcg tttctttcac
    11701 tatcaaagag gtcagagaca aaaacggtta tcttgactct ttagggaaac caagaatcgc
    11761 ccaagtcaaa agagatgcag atatagcaga agcggaagct ctaaaagaaa cgcgtatcaa
    11821 aaaagctcaa gcagaaaaag aatcacaagc agctgaatta cagcgacaaa cagagatcgc
    11881 agaatcatta aaagaaaaag aactgaaatt agcaacctac aaacaagaac aagatgttgc
    11941 taaagcaaaa gcagaccaag cctacaattt agaaagtgcc cgagcacaac aacaagtaat
    12001 cgaacaggaa atgcagatta aaatcgtaga acgtcaaaaa caaatcgaac tggaagaaaa
    12061 agaaatcaca cgtcgtgaaa agcaatatga ttcggaagtg aagaaaaaag cagatgccga
    12121 tcggtacgct aaagaacaag aagcacaagc tcaaaaagtc aaagaagtga ctgaagccga
    12181 agcagaacga ttccgtgtgg aagcactggc agaagcggaa gccaataaga cacgtttagc
    12241 tggacaagca gaagccgaag cagcactagc aaaaggaaaa gcagaagccg aagcaaaaca
    12301 aaagatcgcc aatgccttca aggaatatgg cgaagcagct gtcttgagta tggttatcga
    12361 tatgctaccg caattgatgc gcgaagcagc acagccgcta ggaaatatcg aaaaaatctc
    12421 tgttgtcgat acaggaagca gttcaggaga aaccagtgga gcgaaccgag tgacaaatta
    12481 tgccacaaat ttgctttctt ctactcaaga aacactaaaa gcgacaacag gacttgactt
    12541 gaaggaaatc gtagagaatt tgtcgacacg tgggactact agccaagtaa atatgtatga
    12601 agcgccaaaa gaggcagaac aaaactcgat gaaggaagaa taaagtagta cacttcaccg
    12661 ttcaatcaaa aatggaggaa gactttttaa agttcttctc tccatttttt tattttattg
    12721 caaaagaaaa ttagtcctcg ctatttttct tttgtttttg gatcttagct tctatctctt
    12781 gtaaatttgt tcttagcaat cgttctttct tttggagttc gaccaaatct tctgatagtt
    12841 cttccttctt ttctgtttca ggaggacgaa gaaagaaatt gataccgatt gcaatgaatg
    12901 tccctataaa agtatctagt acacgttgga tagcatacaa agcggattcg ccttgaggga
    12961 tgctcaacgc aatcagcaac aacgtagcta ttgcggaaat gataccggaa ttattgttaa
    13021 taccatctga cacgacgatc acaataataa ctagaatagg aagcaagatc aattctacca
    13081 aaaaatcatt gtgaaaatag cttttgacaa aaaaataaat gatagctaat gtaccaccta
    13141 atgtattgcc gatgatccta gactttccaa aagaaacggt tgtagtcaag tcctgacgca
    13201 aagaaaaaac agcagataat gcagcaatca tcgggattcc tcgttgagtc aaatgaaaaa
    13261 acaagataca taaaaaaaca gctaaggcac ttttgatcgt acgcatacct agtcgaaaac
    13321 ggccaaattg catagagatt ctcctttttg gtctttttac tatcatacct aaacttttgt
    13381 ctgatagcta gtttttcatt attcaagtat aattaaaata aaaaaggggg aagtaaatgc
    13441 gtataggaat gcgaacagtc aaaacagtga tcagtgtctt cttttcgatg gtcacagcgg
    13501 gtagcttatc gctgttgtat tggccagctg cagggatcat tgcattatta agtgtaggaa
    13561 ataccaagcg ctctacattg cgtacgggca tgaatcgaat cgcagccttt atattagcta
    13621 cggcaacagc actgttcagt ttttttgctg ttgggtatac tatttttggc ttcagtcttt
    13681 ttttattcct gttcatcccg cttgcctctc gcttcaaatt aaccgaaggg atagtagtaa
    13741 attcggtttt ggtcactcat tatttagtgg aggaaaacat gtcttggcag ctgatcggca
    13801 atgaagcggg attaatggcg attggattag tatttgcttt gcttgcaaat gtttacatgc
    13861 cagatcgtac gaagcagctg aaggaaaacc aaatacagat cgaaaaagaa tttcgaaata
    13921 ttttgagaca aatggcagaa tttcttttat cagaaaataa agaagatgtg cagatcaaat
    13981 gcgaacattt actgacattc attcgcgaaa gccaagaaga tgccagagaa catcaagaaa
    14041 actactggct cagacagccg ctatactacg aaacttactt ctctatgaga agagcacagg
    14101 ccaatgtgat taaagacatg ctggaaaatc tagagagaat ccagcaacca gcttattatg
    14161 gaaaacatat ctatggttta ttgatttata ctgcagaaac attttcagag tcaaatgatg
    14221 gcagacaaat tttaacacgt attgaagaag tttatgtatt gtatcggcaa atgcctctgc
    14281 cgactagtcg ccctgaattc gaggatcgag cagaattgtt ccagtttttg cagtctttta
    14341 aaagttttat tgaaatcaag gcagaattct cccaacaaaa aatccaaaaa tagaggttgt
    14401 gaattcagct aatattcagg tcaaaaagct gatttcacaa cctcttcttt aaacggtgtt
    14461 ctcctgtcaa ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgtttttt
    14521 gaaattgctc cttgtgactc ggagctgaac ggtaggctca caacctcttt cttaaacggt
    14581 gttcaaaagc tttcctctgc ggtaataagt ccagccaaac gaaaaatagg aggagctatt
    14641 tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct tttaaaatta
    14701 catatttttc catagaactt gcatttgatc caagatttcg atgacatctt ttgtcaagaa
    14761 gagagagcga ttgggcaagt caccttcaat catgtgtacc atattcttta tttcgtaatt
    14821 cattgcttca gatgtattcc cgctttcgat gaattccttt gtaccatctg taaataagat
    14881 ctccgcacga tcagcacgcg ggtaatcagc aatcgtcaca taaccgttct caaaggcaac
    14941 gataccgact tttggcattt ttgcctgaaa agtcaagctg acagcagcaa gttcattttc
    15001 tttgttacgc aagatagtga cggattgttc gtccacgcct gtttcaaaag gaaccattgt
    15061 agaggcaact acttcaggtt gagagctcaa aaagaaacga gcgaaagata cagcatacgt
    15121 accgatatcc aacagagctc ctccagctaa ttctggattg aagaaccggt ttttagggtc
    15181 tggttctttg taactgccga aaggtgcttg gatcattttt aaagccccca gttttcctgt
    15241 atccattatt gagcgtaact gctgataaag tggcatgttg aagattgtca tcgcttctgc
    15301 taagatcaaa ttcttttctt ctgctaagcg catagcgtca gccagttctt ttttatttat
    15361 cgtgatggct ttttcacata gaacgtgttt gcctgcttcc aatgctgcca agatatggtc
    15421 gatatgctgt ctatttggta cagcaatgta cacgatgtcg atttcagcat cgtctaacat
    15481 ttcttgatag cttccgtaag cttttggaat attgtaacgg tgagcgaagt tttccgcttt
    15541 ttctgaagtt ctggaagcaa ctgctgttag ttcgctcgtc tcttgattga agctttcggc
    15601 aaactcatgt gcgatggtac caagaccaac gattccccat tgatattttt tcatctgaaa
    15661 acgctccttc atagttggat tgagtttctt gtctgtctat tattataaca aaaatcacga
    15721 agaataaaga ggagaggact tgaaacatta aaaaaaatga aagcagcgat ttaagaggtg
    15781 ctttttgatc aaaacttaag tagaatagtt aaaaggaggg gataaacgtg catcaagtat
    15841 atattgatct tattatgagt gtattattag aacagtttga aactgaaaaa aattttttag
    15901 atgaatactt gctgatcagt ccagaacaat ggcaaaaatg gaaagatggc gagaaaaatc
    15961 tgccatctga agcgatgcag aaagtaaaaa atcttttcac tgattatgaa tggatgctaa
    16021 cgcaaaagat tttgcgtcaa acgattattt ttcccgaaaa acgaaacgtt gccgttgcag
    16081 agtacaagca aatcaaaaca cgcatcgcgc aaaaatggct tagcagcaaa gcagcaaaag
    16141 tcgagctgat ccctttgaaa aataatcagc aggctggtag ataccttgac ttgaaagtaa
    16201 gtattcaata tgatgaatgg ggctatgatg atattttgaa ttttcgtctg ccagcagatg
    16261 ttcaaaaaca gatcgaagga gaaaaaatcg aacttctcga ttgggtgaat gaaaatctgg
    16321 aagaaactta cgttcatgag gcgaatgggg aacgtgaaga acaacgaaag gatttttcaa
    16381 gtgaataaat ttttaggatt tggagcagtt gtgattatga tcgcagcaat gggctatgtt
    16441 atttttagcg atgatgattc gcatgttcaa gccggcaatc aagcagtcga acgaacaagc
    16501 accaaaacaa ctgctagtaa agaaaagaac agtcaaacaa ccagcagcaa ggtaaaggac
    16561 aaagaaacaa aagatcgtac gtccaaacaa ttgccagatg ttaaaacaga cgattggaag
    16621 ctagttttgg ttggtccaga tcacaaatta gaaaaagaaa ttgatgagtc tactcaattg
    16681 gcatcattaa gtaacggata cttgatcgac aaacgcattg ctccagaata tgaagcattt
    16741 gccaaggctg ctgaagcagc tggatttcca ttagtgatgg tctcagctta ccggtctgta
    16801 tcatcccaac aacaagtttt cgaacaaaat gtccaagatg tgatgagtcg tcaaggtgta
    16861 tcagaagagg aggcgacaaa gattactaaa gaaacgatta cggagccagg atacagcgaa
    16921 caccacacag gtctggcagt cgatgtcgtc gatcaaaact ggtacaacag ttatccttct
    16981 actgtattgg atgcaagtta cggagatcag cctggagcta aatggatcgc agataatgct
    17041 gcgcagtatg gatttatcgt tcgttatcca aaaggacacg aagatataac taagatcaca
    17101 tatgaacctt ggcacttacg ttatgtcggt aaagaaaacg ctgagtatat caccaaacac
    17161 caattgacgc tagaagaata tctgaatgaa ttaaatgaaa agtaggaatt gatcatggcg
    17221 aaaaaaaagt ggaaaaagca aaagcgcaag aatatccgtt ggccagcagt ggttgccggt
    17281 gtagcagtga tactcttagc ctttgttttt tccttgaaga atctgtcttc tccttttact
    17341 gataatcagc aagaaaatga agcgcaatcc catcagcaat ttattgaacg gcttgttccg
    17401 catgcccaag aactacaaga tggttatgga gtgcttccta gtatcatttt aggacaagct
    17461 atattagaat caaattgggg aaaaagtacc ttggctagca aatacaataa tcttttcggg
    17521 atcaaggctt atggggatca gaagaaagta agcctggaaa caaaggaatt tgttaacgaa
    17581 gaatggataa ctatccaagg agacttcaaa gtgtatgatt catgggaaca gtccatggat
    17641 gaccatacac aactgttcgt ccaaggagta gattggaatc ctgcgctgta tgaaaaagtg
    17701 attacggcca ccaactatca ggaagccgca caagcattgc aagatgcagg ttatgcgaca
    17761 gaccccggtt atgcgcaaaa aatcatccag gtcatcgaga cgtatcaatt gaatcagtat
    17821 gaccggagat agcaaaggag aagtggctga tggcagaaca atttacgcca gaaatcatga
    17881 cggaattgag aaaagatatc gtaagagtac cggaaatcat tttagaagca agcgggatca
    17941 agatatttgg aaaattgatc cgttcgatca tctttactac ggatattgcg atcatacgta
    18001 atacaaatgc gaatgcagta attgctgtgt atccttttac acctcatcca gcgattacta
    18061 aaagcattat tgaggcagct gacattccag ttttctccgg tgtaggtggt ggtttgacac
    18121 aaggtgctcg ttcttcctac atgagcatgt tcgcagaagc gcaaggttcg ttaggggtcg
    18181 ttttgaacgg tcctacgcct ttagagacag taaaagaagt ttgtcgagta gttgatattc
    18241 cagttatcag tacggtaacg tcgaaatata cagaaataga tgaaaagtta gcagctggta
    18301 tttcgattat caatatcagt gcaggtaaag aaactgcaga agcagtacgc cattttcgta
    18361 aaaaatatcc tgaacttcct atcattgcga caggtggtcc aacggaagaa agtattgctg
    18421 agaccattca agcaggagca aatgccatca catatacgcc accaagtagc ggagagcttt
    18481 ttagtcgtaa aatggaaaaa tatcgggaaa ctgagaaaaa gaatcatgaa taacaggtga
    18541 gaatcaaaag acgaggaaga gcagatagtg gctcttcctc gtcttttgta ctccagtact
    18601 atttgttcag aattcaaaaa aaagagaaat ttttttgttt ttttacctgt aatgtacata
    18661 agaagagtta aaaccttttg ttattattag cattctaaca aaaaaatgtc ttgatttatt
    18721 gaagcgcttt ctatagaatg aaaaacgaat aaaaactaaa aaaggcaccc gacaatttag
    18781 tagcagctaa attgtcgggg cttgaaaagt cagcgggaac tgaccaatca agttgccata
    18841 gtcaatgctt gtgcatcgac gtgtatattg tacctaattt catacagaat tactagttat
    18901 ttttctgttt tttagggaag agcgcacacg aagaagcatg cttatttagc tatgtcaagg
    18961 atattgggca gttgtctgat atcctttttt ctttgcgtaa ttatcctccc atcattgaag
    19021 caagaagtgt ttttagcgat gaaagaagaa tcagcactat ctaatttttc ttgaaagtat
    19081 tgagttgttc atcctaggca gaacgataag gcaagtcttg gtttttattc cccttgaaaa
    19141 agatcgttta ataaatatgt tctgtataaa aatacagtat attgaaaaat aacaatggaa
    19201 gtagaggtgc agagaatgaa gaaaagaatg agaatcatct tagctttaag tgttatgtta
    19261 ttcttcttga cggcttgtgg gcaagaagat gacaaaaaaa tcacgctgaa catggcttgg
    19321 tggggttcgc aagtccgaca cgatgcaaca gtaaaagtaa tcgagcttta tgaagaaaaa
    19381 aatccccatg tgacgatcga ttatgaattt tatgattatg aaggatatta cacaaaatta
    19441 ggtacactag cagcatcaga tgatatctgg gacattttcc aactgaacaa tcaatttcct
    19501 caatacatca atcagattga accattagat gaatatgtgg agcaagggat aattgatatt
    19561 tctaatgcag atgaatccta tttagcaacg acgacttacg acgggcaatt agttggactg
    19621 tctaacgggg tgaattcgtt tgcgattgct tacaataaaa atatatttga acaattaaat
    19681 atccctgaac cagcttcaaa ttggacatgg gatgaatttg aacaaatcag ctatcaaatc
    19741 actgaagaat taggaatcta tgctatttca agattcgaag attttatagc tggctgtatt
    19801 attcaaatac cacaagttga gaaaggattg aatttttata atcgggaaga tctatctaac
    19861 agcttgggat ttgaaaatcc agactatcta acagattttt tccagctgcg taaaaatctg
    19921 gtagacaaag gggcccatcc agaaccaggt ggtggagctg caagtacgac ggatctgtct
    19981 tttcaggcgg ttagggatag agaagcggca atggtgtttc tatcaagtaa ccagttgaca
    20041 gaaatattgt caggggctcc tgaagatatg gagattcagt tgattaatcc acctcgcaga
    20101 aaagcagatg gagaatctgg tatcccttta cgttcttcgc aaatgctgag tatggcaaaa
    20161 agctcagccc ataaagaaga agctgctaag tttattgatt tctttcaaaa cagtgaagaa
    20221 gccaatgaaa ttttaaaagg agaacgtggt gtaccaatca tgtcgaatat tcgagaacag
    20281 ctcgctcgtg cagatgatgc aactttgcaa gcgacatttg attatttaga aatgatttcc
    20341 gatatggaca acggcgaagt gaatgtcttt gatagtgctt tgaatccgga aattcaagac
    20401 caatacaatc ttttagttga aaaagtcatt tatgaagaag taacagcaga agaagcagca
    20461 gagagtcttt atgagtttgc gtcaaacatt ctaggatagc agcaggagga aggcatgaaa
    20521 aaagaaaaac taaaacggtt cttggatcaa gacaatgtgg tcggttacgt atttgcttct
    20581 ccattcatcc taggatttct attgataaca gtgataccga tgatcttgtc actttattat
    20641 tcgttcaccg attatcgttt gggaaatcca gtctcatgga tcggcttgga taattatata
    20701 aagatgctga gtgatgcccg ttttttgaac tccatccgag tcacttttct ctatgtattt
    20761 gcatctgtac ctgtgaaact gatctttggt ctattgattg cttatctttt aacgaggaat
    20821 atcaaaggaa cgactttctt tcgttctttg tattatgtgc ctagtttgat cggtggcagt
    20881 atttctatcg ctctagtttg gcgggagttg ttttctaaaa acgggttagt gaatattttc
    20941 ctggaacgat taggcgcacc tgcgctttcc tggtttagcg atcaaaaact ggtcatgatc
    21001 cctcttgttt tgatgtctgc ttggcaattc ggttcggcaa tgatcatctt tgctgcaggt
    21061 atcaaagaga tcccaggctc gtattatgaa gcggcagaga tcgatggagc gaacaaatgg
    21121 cagacgtttt acaagatcac gttacctctt ctttcgccga tcattttgta taacttggtt
    21181 atgcagacaa tcgcggcgtt tatgactttt acgcaggcat ttatcattac ccaaggtggt
    21241 ccgaatgatg ccacgaactt ttatgctctt tatgtgtata accaagcttt ttcttttaat
    21301 tctatggggt atgctagtgc tatttcgtgg atcatgctgt tgattatggg agctgtcacc
    21361 tttctgattt tcaaatcatc aaaaaaatgg gtcgttgtgt gaatgagagg aggaagaaat
    21421 gaataataca gtaacgaaat ggtttcaata cggttttatt ttgctattag gtctagtcat
    21481 gatttatccg gtcttatgga tgatcgcagg ttcttttaaa acggatcaag agattatgag
    21541 tggttcgctg aatttgattc cagagacttt tcaatgggaa aattacgcaa aaggatgggc
    21601 ggggtttgct ggaatttcat tttttacttt ctttaggaat tcgtttgtca tctctgcagt
    21661 ttcgacgata gggacagttc tgagctcgac ttgtattgct tacgcattat cgagaatcga
    21721 tttcaaagga aaaagattct ggttcgtcgt catgttagta acgatgatga ttcctgcaca
    21781 agtgattttg attccgcagt ttattattta caatcggctg aatttggttg ggacgtatgt
    21841 gccgctgatt ttaccacatt tttttggaca agcatttttc atttaccaga tcatgcaatt
    21901 catggtcagc atccccaaag agttagatga atcagcgatt attgacggat gcagcaagta
    21961 tagtgtcttt accaaaatta tttttccttt gctgaaacct tcgatcatca cgacgatcat
    22021 catccagttt tattggaaat gggacgactt catgggacca ttgatttatt taaacaagcc
    22081 gagatcttac acggtatcga ttgccatcaa attatttgca gacgctggat ctagtacgac
    22141 ttacgcgagt atgtttgcga tgtctacgtt gtcgctgcta ccggtttttc tgattttctt
    22201 attcttcaat aaatatcttg tccaggggat cagtacaagc ggtctgaagg gctgaatatg
    22261 aaaaagccaa tagaagagat gtttctttac aagctcaact actatgtctg tcggattttt
    22321 ttaaccagtc ttatcagtaa tgctcttttc gctttagttc taggatgttt gttcacttta
    22381 ggattagtgc tgtctgctcg tgttgtctat ctgatttatt ttttgatgat gtatccttct
    22441 tattatctat ctgtccatta cttggcagaa aaaaaggtat gttggaggaa aggaaaaatc
    22501 aaacggatgt gtttcatcat tacgttgttt cagctgtttg tcattatttt tgcagttgat
    22561 tggtatttat tagtgggaaa gattggagct tggtatctga ttccttttct attgtgtctg
    22621 ttgctttttg tatcccatag tttttttagt acgctaattg atatctgcaa agaagataaa
    22681 gcagatagtc ttgtttattt gatcaaaagg aatatctggc tgttgtcaaa aaacgtcaag
    22741 gggtttctgt ggtatttatg ccttttgtat ggactgttct ggtgtataca gcatgatttt
    22801 ttactcactg cattactgta tccagggctg ccatttgtca cagtattttg cttaaaagaa
    22861 aaggagaatg actttaaatg aagaaaaaat atgtacaagt cggacttggt gggagagcgc
    22921 gtttttttta tgaggcaatt gcctctacgt atcatcaaac ttgtgaacta gtcggttttt
    22981 gtgatgtatc tcaaacaagg atggattatg caaatcggat acttgtgaac aagtatggac
    23041 agaaaaaagt aaagacatgt atgccatcac aatttgagca actactggat gacgtacggc
    23101 cggctgaagt cattattacg tcagtagatc gaacgcatca tgattatatc attcgtgcaa
    23161 tgaaaaaagg atatgatgtt atttgcgaaa aacctctgac tatcgatgag gaaaaagctc
    23221 aagaaataat aacggtccaa aaacagacgg gaagaagcct gcgagtcact tttaattatc
    23281 gttatgcacc gtatcacacg aagataaagg aactgataga aagtgatgtg attggtaatg
    23341 tgttttctat ccacttcgaa tggctgttaa atacgcaaca cggggcagat tatttcagaa
    23401 gatggcatcg aaataagcac aatagcggcg ggcttcttgt tcataaatct acccatcatt
    23461 ttgatctagt gaatttttgg ttgaacagcc gtccccaaaa ggtatttgca ttcggtggat
    23521 taaactttta tgggatgcat aatgcggaga aacgcggagt agaaatattt tacagtcgat
    23581 gctatggaag cgaactagct aaagctgatc catttgctat agacctgtca gccaatgagg
    23641 aattgcgaga aatgtattta gaagcagaaa aagacagtgg atatattcgt gaccgcagtg
    23701 tattttccga tgatatctct attgaagata cactaggcgt aacggtcaaa tatgaaactg
    23761 gtgcgatttt gacgtattcg ctaaacgcgt atatgccgtg ggaaggattc aatgtttctt
    23821 tcaatggcag caaaggacgg atcgaaatga aagtgatcga aaagtcttac atcaatgcag
    23881 gtggagataa aaaggatgaa ggagcagccg ttcttcagtc catccatgtc tatccgatgt
    23941 tcggtgagcc atatgaggta atcgtcgaac aagaagcggg cggacatggc ggaggagatc
    24001 cgcgtttatt agatgatcta tttggtgaag aaaaagaaga agatccgtgg aatcgggcag
    24061 caactcacgt agatggtatt ttgtcgattt tgacaggaat cgcagcaaat cattctattg
    24121 cttcaggaaa agcagtagtg attgacgagc ttgtcagctt aaaagaataa gaatagattt
    24181 acggaaatta cttcccgtag tttataaaat cttgccttat tgccgatagg ctgtcttttg
    24241 aacactattt attcggaaaa aggggctatg acatgctttt tgtcatgcct tctttttatg
    24301 tgggctgctt ttttagatag gagaaaataa aaataactat ttaaacccga acaaaaagga
    24361 ataatgcatt aaaaatcatt agattttaca aaaaaataag taaaccacgt tgtcagtttc
    24421 agaaagtagt gttagactgt tttcaatatt tgaaacaaca ttttttattc aaatatggca
    24481 aactgccttc acgtgcaaaa cagttgcaag aaaatgaatt tacactatta ttagttttac
    24541 atacctactt atatatcgtc ataatatgga tgacagtttc tagccagtac cgtaaatgct
    24601 ggactataag taaaagattg gctatttcac ccttcttttc ttatgataaa gtggagggtg
    24661 tttttcttta acagaaacat agtaggtttt tgcacaaaag cggttatttc ggtattaata
    24721 aatgataaag gagcaaataa agtgaaagaa ttagttgaac gtattcaaaa agacggacgt
    24781 atattaggcg agggcgtatt aaaagtcgac agcttcgtca ctcaccaagt agaccctgta
    24841 ttgatggaac aaatgggaaa acggtttgca gaagtattta aagaacaagg aatcacgaaa
    24901 gttgtgacca ttgaagcttc tgggatcgca ccagctttat ttgctgcaag agaattagat
    24961 gttccaatga tcttcgctag aaaagcaaaa agtttgacga tggatgaaga attattgaca
    25021 gcttctgttt attcttttac gaaacaagtg acaagtacga tttctatttc tcgtaaattt
    25081 ttatcttcag aagacaaagt attgattatc gacgatttct tggcaaacgg acaagcggca
    25141 aaaggattga tcgaattatg ccagcaagct ggagcgcaag tagaaggaat cgggatcgtc
    25201 atcgaaaagt ctttccaaga tggacgtcag ttacttgaag aaatgggatt gagagtcgtt
    25261 tctcttgcgc gtattgcttc cttgacgaat ggacaagttg agtttcttga ggaggacgca
    25321 taatcgtgga aaaagaaaca caaaatggaa aagctgcggt tttagggctg cagcatctgc
    25381 tagcgatgta tgcaggagca gtagcggtgc ctcttttgat cggtacagga ctaggcttta
    25441 atcaagaaca gatgacttat ttgatttcaa ttgatatctt tatgtgtgga ttagcgacat
    25501 tattgcaatt gacagttaac cgatttttcg gaattggctt gcctgtcgtt ttaggatgtg
    25561 cgatccaagc agtagctcca ctgatcttga tcggaaccaa tgaaggagta ggagctatct
    25621 acggatctat tatcgcttct gggatctttg ttgtgttagt ttctggtgtc ttttctaaaa
    25681 ttaaaaaact attccctcct atagtaacag gaacggttat caccgttatt ggtttgactt
    25741 tgattcctgt agcaatcgag aaaatgggtg gaggtaatgc aacagcagct gattttggtg
    25801 acaaaacaaa tctgctactt gcatttgtga cgattctcct aattgtaggc gtgcaaatgc
    25861 tggcaaaagg gttcattcgt tcaatcgcgg tattgattgg tttagtcgga ggaagcatct
    25921 tagctgcttt catgggaatg attgatctga gcgcgattgg agatgcgccg ttgttccatg
    25981 ttccacaacc cttttatttt ggtaaaccaa ctttcgacgt atggtcgatt ttactaatga
    26041 tcatcatttc catggtcagc atggtagaat caacaggtgt atattttgca cttggtgaca
    26101 ttactgggaa aaatgttgga gaagaagaat tgaaaaaggg atatcgtgca gaaggacttg
    26161 ccgttatctt aggcggtatt ttcaatacat tcccttatac tggcttttct caaaatgtcg
    26221 gattggttca actttcggga atcaaaacaa gaaaacccat ctatttctct gcattcttct
    26281 tgattgtttt aggattattc cctaaaatcg gtgcgttagc acaaatcatc cctgagccgg
    26341 tacttggtgg aggaatgtta gtcatgttcg ggatggtggc agtccaagga atccgtatgc
    26401 tgttggaagt tgatttcagc aatgataaaa atctattgat tgttgctgtt tctgttggat
    26461 ttgggctggg tttcaatgtt acgccgacat tattcagtca aatgccgcaa acattgcaaa
    26521 tgttcactgg aaatggcatc gtaatgagta gcattacggc tatcatattg aatttgatct
    26581 ttaatggatt gaaaaaagat gaagaaatat tggaagaagc cttcaagaag taatagagca
    26641 aaagaaaggt tgtgaaatca gcgttttgac ctgattgtta gagaaacaaa cggaaaaata
    26701 gttcctcgta ttttgccgtt tgtttgagct aataccgagg gtcagctgat tgaacaccgt
    26761 tgaaggaaga ggtcgtgagc ctgccgttca tctccgagtc acaaggaaca atttcaaaaa
    26821 aaagcttctc atgtttgttg aatattgttc cttgtgaccg aggagttggc aggagaacac
    26881 cgtttatcca taaaaaggaa tgtgacctaa cttttgtcac attccttttt tttatcttta
    26941 ataaacacga acaataaaat gtttttcttt gttaacattc gaaatttctt tgacatcaaa
    27001 aaaagtgatt gttaaaatat gaatgtagaa aaactgggag gaaaaacaat gacagcaaaa
    27061 atctctgtga ttatggggag tacttcagat tgggaaacaa tgaaagaagc atgtcaaata
    27121 ttggatgaat tcggtgtgtc ttatgaaaaa aaggtcgttt ctgctcatcg aaccccagat
    27181 ttgatgtttg aatatgcaca aaacgctcga aaaaaaggaa tcaaaatcat tattgcagga
    27241 gcagggggag ccgcgcatct gccgggtatg gtcgcatcaa aaacaacttt gccggttatc
    27301 ggtgtaccgg tccaatcacg aacattaaat ggtttggatt cgcttctttc gattgtccaa
    27361 atgcctggag gagtacctgt agctactaca gcaatcggaa aagcgggagc aattaatgct
    27421 ggactacttg cgatacagat gctttccatg tatgatttag aattggaggc taaattggct
    27481 gaaagacgtt caatgttggc taaaactgtt atagaaagta gtgatcaact tggttaaacc
    27541 tttgttgcct ggttcaacga tcgggatcgt cggtggcggt caattaggtc gtatgatggc
    27601 gattagtgca aaagaaatgg gctttaaagt aggtgtgctg gatcctgtga gcgattgtcc
    27661 agccgcacaa gttgcagatt ggcatattga aagtgtctat gatgacacct ttgcgctgaa
    27721 agagcttgcc agaagaacag atgtcattac ctatgaattc gaaaacgtca gtgtagaggc
    27781 gttaaatgcg attttaccca tgtcatttat tcctcaaggg acagacttgc ttgcgattac
    27841 gcaagaccgc ctgttagaaa aatctttttt ggaaacgaat aacatcgtga ttgctcctta
    27901 tgcaacgatc gtcagtccga cggatatcca agatgcgatt gacgggatcg gctatccttg
    27961 tgttttgaaa accactcgag gcggctatga tggcaagggg cagtatgttt tgaaaagtcg
    28021 ggcggatcta gctcctgcca tggacctttt aagagaagga acctgtgttt tagaagcatg
    28081 gatcccgttt gaaaaagaga tttcgatcat ggtggcagga aatggtcaag gagatttcac
    28141 gacttttccg gtcgtggaaa acatccacca caacaatatt cttcatgaaa cgattgctcc
    28201 agccgcgatt gatcaggatg tgatcgagga ggctgagcgt attgcccgtg tgattgcaga
    28261 agcagtgagc ctttcaggtg ttttaggggt agaaatgttt ttaacaaaaa caggcgggct
    28321 atatgtgaat gaattagctc ctcgtcctca caattctgga cattattcta ttgaagcttg
    28381 ttctatgagt cagttcgatg cccatattcg aggtatttgt ggttggccat taggagaaaa
    28441 agtacgtttg ataagcaatg cagtaatggt caatgttttg ggagaacaac tgctggaaag
    28501 ctatcgcttg attgagaata agccagactg gcaattccat ttctatggaa aaaaacaagc
    28561 aaaaaaaggt cgcaagatgg gacatatcac tatcttgaca gaagacatcc agcaaacgct
    28621 ggaagaaatc aaacaaacga atatttggga ttagaggaga aaaaacatgt tagaacgtta
    28681 tacacgccct gaaatgggga atatctggac ggaagagaac aaataccgtg cgtggctcga
    28741 agtcgagatt ttagcagatg aagcttgggc agaacttggt gaaatcccgc aagaagatgt
    28801 gaaaaaaatt cgtgagcatg catcatttga tatcgatcgg atcttggaaa tcgaaaaatt
    28861 aacacgccat gatgtcgttg cgtttacacg agcggtttct gaaacattgg gagaagaaag
    28921 aaaatgggtc cattatggtc taacaagtac ggatgtcgta gatactgctt atggctatct
    28981 catcaagcaa gcaaatgata ttttacggga agacttgaaa cgttttacgg aaatcattgg
    29041 cgaaaaagca aaagaacaca aatatacagt gatgatgggt cggacacacg gtgtccatgc
    29101 tgaaccaact acttttggat tgaaacttgc cacatggtat tcagaaatga aacggaacat
    29161 cgaacgattt gaacatgcgg caaaaggagt agaagcagga aaaatctcag gagcagtggg
    29221 aacgttcgca aatatcccgc catttattga agagtatgta tgtaaacaat tggggatccg
    29281 tccacaagaa atttctactc aagttctccc tcgggatcta catgctgaat atttctcagc
    29341 gatggcgctt attgctacaa gtattgaacg ttttgcaaca gagatacgcg gattgcaaaa
    29401 atccgaaaca cgtgaagtag aagaattttt tgcgaaaggt caaaaaggtt cttctgctat
    29461 gccgcacaaa aggaatccga tcggttcaga aaatatggct ggtctagcca gagtggtgcg
    29521 cggacacatg gtcactgcct atgaaaatgt cacattatgg catgaaagag atatttctca
    29581 ttcttcagca gaacgaatca tcttgcctga taccacgatt ttattgaatt acatgttaaa
    29641 tcgttttggc aatatcgtta aaaatctaac ggtctttcct gaaaacatga agcggaatat
    29701 gtctgcaaca tttggtttga tttatagcca gcgcgttatg ctgaaattga tcgataaggg
    29761 aatgacaaga gaagaagctt atgatcttgt tcagccaaaa actgcgtacg cttgggacaa
    29821 ccaagtgcag ttccgcccgc tgttagaaga agatccaacc atcacatcgg tattatctaa
    29881 agaagaaata gacgatgcct ttgattatca tcatcatttg aaaaacgttg acgtgatatt
    29941 caaacgagta ggtttggaat aaagaagagt gagttgcaaa attcaattaa aatagaatag
    30001 caaaaataac caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata
    30061 ttttcttatc aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt
    30121 tacatacaaa aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg
    30181 gttatggaga tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa
    30241 taagatctgg atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga
    30301 aagttcgcct ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg
    30361 tttggttgct cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata
    30421 aacgtctttg atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc
    30481 tgataaaaaa agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc
    30541 cattatccaa atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt
    30601 gttgatggta taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct
    30661 taaatgtaca tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa
    30721 acgaacaaaa aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag
    30781 aagaaagaac ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac
    30841 ttgatttgga tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag
    30901 ataagtggaa aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact
    30961 caaagaggaa taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa
    31021 aaagtacaaa ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca
    31081 aaacgtctag gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat
    31141 aaggatgacc agttggatga tccatttatc aatgaagagc atatctattt cttacaactt
    31201 gcaaagccgg aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg
    31261 accgaattat ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg
    31321 tcgaatggac aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat
    31381 gaacgtacga atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc
    31441 ccagtctatc aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta
    31501 tgatgtattt cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg
    31561 aggcagtaaa aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag
    31621 gaaaatactt tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa
    31681 cgatttgtga caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa
    31741 aaatggagga agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac
    31801 gcagatatgt tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat
    31861 gatgagtcct ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt
    31921 gactatttga gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt
    31981 tttgctgaag aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa
    32041 gcaggacttt tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact
    32101 gtaccattga aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata
    32161 atctatcttc cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa
    32221 gaactaaaaa aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg
    32281 gaaaacattg cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca
    32341 gaacgtgcag tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt
    32401 ttaaaaaact tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa
    32461 gagatcaaga aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg
    32521 atcgaagaag atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt
    32581 atgtggagcg aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact
    32641 agtggacctc atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat
    32701 ggactagcag ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat
    32761 gaaggagctg cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt
    32821 ccaattgcgt tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat
    32881 ttactagaag aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact
    32941 gtaggcggcg aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg
    33001 tgcgtaggtt tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat
    33061 tcgatcatgt atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc
    33121 tcagaagaat ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct
    33181 tttatggaaa agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct
    33241 ctaataggta tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct
    33301 tcaaaagctg gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg
    33361 atgacacctt atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa
    33421 tcaggagagg aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact
    33481 atcggtaaag taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca
    33541 aatctgccag tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa
    33601 ccagctcgaa tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct
    33661 ggacaagtat tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac
    33721 gaaacttatg attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct
    33781 gtgatgaggg ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc
    33841 tatctgtatt tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat
    33901 attattgcta gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct
    33961 gataaaccag aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt
    34021 gaaacgttag gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt
    34081 caagcaatct atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat
    34141 atcactactc aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt
    34201 gcagattttg atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc
    34261 cgaaactttg atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa
    34321 aacggattga ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca
    34381 gaatcagctt tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa
    34441 ttatttgcag aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga
    34501 ttcgaagaaa tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata
    34561 atcgaaattt cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc
    34621 tgggaggatg caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct
    34681 tcggtgtttg gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc
    34741 agcatcgagg acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc
    34801 atcgagattt ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa
    34861 caggagaagc tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca
    34921 atattcagcc ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta
    34981 acttgatcaa tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt
    35041 ctaactccga tacggagata ttgatgcatt tgatccgacg aagtgaagaa acaaattttt
    35101 taaataaatt aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga
    35161 cagaaacggc aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac
    35221 aaatgaaaaa cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg
    35281 cacgttttat aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc
    35341 agattgattg ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt
    35401 ttgccagacc agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg
    35461 gacggaattt agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt
    35521 cttctttgtc agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat
    35581 tagtaaaaaa tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc
    35641 aaggtgttcg aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta
    35701 tggtcgatga ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag
    35761 aagcaggggc aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt
    35821 tttacgggat tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg
    35881 aaatcaaaga atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg
    35941 aatcgatcgg tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg
    36001 gagattatcc gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa
    36061 aagtatcgtt cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca
    36121 tacgcaaaag ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag
    36181 catgtcaaaa aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt
    36241 tttgatttat cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg
    36301 gggacgaaat tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc
    36361 gtagcgatgt gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac
    36421 tacttagcaa cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca
    36481 gatggatgtg ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt
    36541 atgtactcag gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct
    36601 caattattga caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc
    36661 gggattcatt caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt
    36721 tctttatcat caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca
    36781 acgaaaatct atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct
    36841 gcacatatca ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg
    36901 gctgcacaaa tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa
    36961 tatggtgaaa ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc
    37021 ctggcagtcg aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa
    37081 aaagtttatg aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga
    37141 gaagaacgat gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag
    37201 ccgattatct atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc
    37261 ctgaagcata tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac
    37321 ctaaagaatt tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa
    37381 aaaagatcga tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga
    37441 aaaattatga taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt
    37501 tgcatggcat acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt
    37561 atatcgacca aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac
    37621 aggaggacac gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc
    37681 cagaagtgat tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat
    37741 cagtgtatca gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt
    37801 agaaatcatt tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct
    37861 ttcgatcgaa gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca
    37921 tcctaaaatc catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat
    37981 ggaaacggaa aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga
    38041 aacgattatg aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg
    38101 tcctagtatg ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc
    38161 atctgactat gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt
    38221 cagacaaaaa ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc
    38281 tcaatactta acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga
    38341 acgaaaacaa acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc
    38401 cgctcttctg gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc
    38461 ttataataat atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc
    38521 aacggtagtc gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt
    38581 agaagcgtgg gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt
    38641 tttgaatcga gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat
    38701 catcattgcc ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat
    38761 ccgattactg acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc
    38821 agtcttaggt ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg
    38881 gaaagtcgtg acaaaacgta agccgactcc agcagaagca aaagcattag tgtttgcttg
    38941 gagagctgtc aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt
    39001 aggaatcgga gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc
    39061 gcaagagaaa atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc
    39121 ggttgaatat gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa
    39181 agaccaagat tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt
    39241 tcggcatttt agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg
    39301 tggtcgtgaa catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta
    39361 ttgtgcgcca ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga
    39421 gcgagatcat tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt
    39481 tggtcctgaa gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa
    39541 aatcttcggt ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca
    39601 attaatggat acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa
    39661 agcagcagcg tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc
    39721 agctgggaaa ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca
    39781 aatgttgaac cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt
    39841 ggcaggagaa gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt
    39901 catttcgcaa gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat
    39961 gggtgcttat tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga
    40021 agttttaaaa ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt
    40081 atatgcgggt ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt
    40141 cggtgatcct gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat
    40201 cgatgattta ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt
    40261 aggcgttgtg gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc
    40321 agattttctg gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa
    40381 actagttgga gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga
    40441 agcacagaaa aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg
    40501 tacagatatt ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt
    40561 ccatttataa aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca
    40621 taaagagtgt ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa
    40681 ttcaattagg tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca
    40741 acaatgcgga agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt
    40801 ttgaccatgc agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag
    40861 caaaaacatc aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag
    40921 gtgtgatgtt tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc
    40981 gtctgaaaat ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac
    41041 cagaggaagt agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg
    41101 gcgtcagcaa tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat
    41161 tagtagctaa tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc
    41221 atgtcaatat gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc
    41281 gattgcatga tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg
    41341 tatttatcgg gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta
    41401 aatatcaaac gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata
    41461 tgcaagtgat cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag
    41521 aaattaatat gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac
    41581 cttaaagatt gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga
    41641 aagctgtttt cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat
    41701 tgtacggatt tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct
    41761 tttggagaaa tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa
    41821 ccaatttcaa aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct
    41881 aattcgattc tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa
    41941 atcgtactga aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata
    42001 tctggatcgg taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat
    42061 ttgttccttg aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc
    42121 gaacggtaaa gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga
    42181 accatttctt gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag
    42241 acaaataaac gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg
    42301 atgtacttag atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa
    42361 aagacagaat caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt
    42421 tctttaaggg aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat
    42481 tcgctgattc tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt
    42541 tctaattcat ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca
    42601 agagaatcaa ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt
    42661 tgattctccc taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct
    42721 gtactgactt ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg
    42781 aaagattcag caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc
    42841 aagaaatgat tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc
    42901 tgcaaatcta aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc
    42961 ggtttaagca gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg
    43021 tcgaatccac taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa
    43081 aaagtcaacc cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag
    43141 tcgataattg aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga
    43201 taataagaac caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct
    43261 tcgatgataa aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat
    43321 catcagtgtg acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc
    43381 atttccgtaa taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa
    43441 atctgaatcg atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat
    43501 gccaatcccg ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat
    43561 acgaataaat cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa
    43621 tggctgcaag atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt
    43681 atagtcaagc atatggccgt atcttatttt taagatgact agataatcat tgatatggct
    43741 aatttctttt tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc
    43801 ggaaagtgca tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat
    43861 aatggcatcc aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac
    43921 ttcactttgt gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc
    43981 ttccgccatt ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt
    44041 taaacgaaag ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat
    44101 cggttcttgg acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt
    44161 actaagaact ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt
    44221 ttttttgctt gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag
    44281 ttgagtgaca gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc
    44341 caagatatta ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga
    44401 tacatctaac gtactaattt ctttaagtgt atttgtatta tttttgctct ctcgaatatc
    44461 attcctaagt tcttctagga tattttcctc attgaactca tctacggtta cttggccaga
    44521 aatcaagtcc cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc
    44581 tttttggata tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat
    44641 gctcagtagc aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat
    44701 ctgttttatg cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga
    44761 tgttagctat attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg
    44821 tttttcctgt ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga
    44881 catcaggttt acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat
    44941 cttcttgtcc atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt
    45001 ttatcgcagc taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt
    45061 cggataaatc atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa
    45121 aatcaccgac aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt
    45181 tttttagggt tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg
    45241 gtgtggagtt ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg
    45301 tgaagtgatt gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa
    45361 tgtgggtgac gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag
    45421 ggatccctgc tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag
    45481 taaaaatgat catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt
    45541 cctgattcat gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga
    45601 taattgattc agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa
    45661 cgactagtgg gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga
    45721 ttacaacaag ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt
    45781 gttagtacta tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa
    45841 aatccaagaa acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa
    45901 atcccataaa aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg
    45961 aaatggggga taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt
    46021 gaccgcaagc attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg
    46081 gtatgtagga atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa
    46141 catggtctcg gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa
    46201 agtagaaaat caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt
    46261 gattgcttct atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga
    46321 tatcaaggtg atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc
    46381 gacttttgat aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg
    46441 attgaaagaa ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa
    46501 caacgcacta atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg
    46561 tcagttggtc gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga
    46621 tggttctacg gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac
    46681 attggatgct gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa
    46741 aggcgtgggg tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac
    46801 agttgctggc gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac
    46861 acgtatctta gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt
    46921 tccggtcaat gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc
    46981 aaatacagta tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta
    47041 taaagaatcc gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc
    47101 agagagagga taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa
    47161 ttttctggaa tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat
    47221 gcattgtgcg gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg
    47281 tatccatatg gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa
    47341 gatttgaaag atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt
    47401 ccttatcttt caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg
    47461 attgattggg aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct
    47521 atcgacccga atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata
    47581 gctaaagcat tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg
    47641 aatgaagaag aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta
    47701 acctccatca ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc
    47761 attttacggg atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat
    47821 attattcgcg gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat
    47881 attggagaag tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag
    47941 cgaatcataa acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct
    48001 ggactgatgg gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga
    48061 agcaagatat ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct
    48121 tcagccattg caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat
    48181 ctgctgatgg atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa
    48241 ggagtattag ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt
    48301 ataaaaacaa actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa
    48361 gtcgttttat ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc
    48421 cgtgggatag atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca
    48481 aatggcaaag ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat
    48541 cgaatctata cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat
    48601 caggaagtct tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa
    48661 aaatcttacg gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt
    48721 tggtcgctac agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac
    48781 ggatggtatt ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat
    48841 attagtacta gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt
    48901 agggtcagtg atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat
    48961 gaacccgtgg atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca
    49021 aggtttttgg gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct
    49081 gatgtttcgt ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc
    49141 tgttttccaa aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca
    49201 cttcttgaca ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg
    49261 aacaagaaat cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt
    49321 aaaggcggta tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca
    49381 agggtatccg attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac
    49441 aaataaaaca gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga
    49501 actatcaggg atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat
    49561 ggcagcttta gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg
    49621 tacatcgatg gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg
    49681 cggcattgga acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa
    49741 tggcatgtcg attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt
    49801 gcttttagcc gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc
    49861 cggttattca gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag
    49921 cgaacattca ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt
    49981 ataaattctc tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct
    50041 aaaaaaagcc ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg
    50101 ataatgtaat gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg
    50161 taaatagccg ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc
    50221 cagtagtgcc tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg
    50281 aaaatggtta tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca
    50341 aaaaaatgaa aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat
    50401 attcctttat agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca
    50461 tcgcgcatgg acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc
    50521 ctgaaacttt tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa
    50581 atgcgggcgc agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa
    50641 tcaagacagg ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag
    50701 cagctcgcaa gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat
    50761 ctgtccgttt tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat
    50821 ctcctggcga aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt
    50881 cagaattcca aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca
    50941 aaggatcttt gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt
    51001 atgctggagg aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt
    51061 cgatttttaa tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag
    51121 ttgtcctgca ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt
    51181 ggctggaatt attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg
    51241 tgataagatt tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt
    51301 aaattcggta aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa
    51361 agtagagaaa aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat
    51421 cgaaaacgga aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact
    51481 ttttggtgtg aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt
    51541 actaacgagc aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc
    51601 tgttccaaga atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta
    51661 tgagcaaaaa ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc
    51721 tagcgagatg ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga
    51781 aatcttagcg gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat
    51841 caaacggtat ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac
    51901 tggtggcggt cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg
    51961 gatagctgta gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac
    52021 gcgtttattt gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc
    52081 gatccataaa aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg
    52141 aaaagtgatg agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct
    52201 tccaattatt gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct
    52261 tcctatacga acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt
    52321 aggaaaaaca acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc
    52381 tcaagcgcaa gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa
    52441 tgtatttaat gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa
    52501 aagccgactg ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt
    52561 aggtggttac tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga
    52621 aaaagtagta gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa
    52681 tggaatcaga atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa
    52741 gatgaaaatt ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa
    52801 tataacagct tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata
    52861 taaagaaact aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa
    52921 atgtttgcaa caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt
    52981 ttgattagaa aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt
    53041 attcactaat gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg
    53101 cttttagctt gtgtttctca taaatccatg ataatttatt aatgaagacg gttaaatagt
    53161 gatacagttt tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga
    53221 ggaatgtgtg acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg
    53281 aagtcaatgc agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca
    53341 aagatttagt acctgattta tccgatgatg aactcgttga attaatgact catatggtat
    53401 ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt
    53461 ttgcaccaac tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag
    53521 aagatgttct tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt
    53581 tatctcaagc ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat
    53641 tgaaagctct accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg
    53701 cattaggttt gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg
    53761 gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg
    53821 ccgtatttta tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg
    53881 cagcaaaaac acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg
    53941 gaatggatcc gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg
    54001 gaaatggacc agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg
    54061 gtgacgatcc aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc
    54121 cattgatccg cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag
    54181 aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag
    54241 taccaaaaca aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa
    54301 tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac
    54361 aatgatccaa gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt
    54421 tgtttttggt gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca
    54481 ggaaaagttc ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg
    54541 attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg
    54601 ttttattttc gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat
    54661 gagcggcact agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac
    54721 accagaattg cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt
    54781 agtgattcct tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa
    54841 cgatccggtt gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc
    54901 ggatgaagct tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt
    54961 atctattatc acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc
    55021 aaaagaaaat atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga
    55081 aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg
    55141 acaagcgggc gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt
    55201 agaagcccca atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga
    55261 aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt
    55321 ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa tttaaatt
//
LOCUS       DAXLPI010000004        51916 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXLPI010000004 DAXLPI010000000
VERSION     DAXLPI010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.004"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            159..530
                     /locus_tag="ACF3T5_000179"
     CDS             159..530
                     /locus_tag="ACF3T5_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFD3630822.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(804..1496)
                     /locus_tag="ACF3T5_000180"
     CDS             complement(804..1496)
                     /locus_tag="ACF3T5_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3630823.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1499..2284)
                     /locus_tag="ACF3T5_000181"
     CDS             complement(1499..2284)
                     /locus_tag="ACF3T5_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFD3630824.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2521..3921
                     /gene="lacG"
                     /locus_tag="ACF3T5_000182"
     CDS             2521..3921
                     /gene="lacG"
                     /locus_tag="ACF3T5_000182"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFD3630825.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            3946..4263
                     /locus_tag="ACF3T5_000183"
     CDS             3946..4263
                     /locus_tag="ACF3T5_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFD3630826.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4276..5949
                     /locus_tag="ACF3T5_000184"
     CDS             4276..5949
                     /locus_tag="ACF3T5_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFD3630827.1"
                     /translation="MDGIIKQIEKGKAFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            5963..7594
                     /locus_tag="ACF3T5_000185"
     CDS             5963..7594
                     /locus_tag="ACF3T5_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFD3630828.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8085..9650)
                     /gene="guaA"
                     /locus_tag="ACF3T5_000186"
     CDS             complement(8085..9650)
                     /gene="guaA"
                     /locus_tag="ACF3T5_000186"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFD3630829.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9709..10632)
                     /gene="coaA"
                     /locus_tag="ACF3T5_000187"
     CDS             complement(9709..10632)
                     /gene="coaA"
                     /locus_tag="ACF3T5_000187"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFD3630830.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10828..11433
                     /locus_tag="ACF3T5_000188"
     CDS             10828..11433
                     /locus_tag="ACF3T5_000188"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3630831.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11741..12757
                     /locus_tag="ACF3T5_000189"
     CDS             11741..12757
                     /locus_tag="ACF3T5_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3630832.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            12898..14199
                     /locus_tag="ACF3T5_000190"
     CDS             12898..14199
                     /locus_tag="ACF3T5_000190"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFD3630833.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14245..14907
                     /gene="deoC"
                     /locus_tag="ACF3T5_000191"
     CDS             14245..14907
                     /gene="deoC"
                     /locus_tag="ACF3T5_000191"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFD3630834.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            14928..15326
                     /locus_tag="ACF3T5_000192"
     CDS             14928..15326
                     /locus_tag="ACF3T5_000192"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFD3630835.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15392..16474
                     /locus_tag="ACF3T5_000193"
     CDS             15392..16474
                     /locus_tag="ACF3T5_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFD3630836.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16686..18248
                     /locus_tag="ACF3T5_000194"
     CDS             16686..18248
                     /locus_tag="ACF3T5_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3630837.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18241..19374
                     /locus_tag="ACF3T5_000195"
     CDS             18241..19374
                     /locus_tag="ACF3T5_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3630838.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19374..20330
                     /locus_tag="ACF3T5_000196"
     CDS             19374..20330
                     /locus_tag="ACF3T5_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3630839.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20429..21595
                     /gene="deoB"
                     /locus_tag="ACF3T5_000197"
     CDS             20429..21595
                     /gene="deoB"
                     /locus_tag="ACF3T5_000197"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFD3630840.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21619..22437
                     /locus_tag="ACF3T5_000198"
     CDS             21619..22437
                     /locus_tag="ACF3T5_000198"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFD3630841.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22454..23161
                     /gene="deoD"
                     /locus_tag="ACF3T5_000199"
     CDS             22454..23161
                     /gene="deoD"
                     /locus_tag="ACF3T5_000199"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFD3630842.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23459..24145)
                     /gene="gpmA"
                     /locus_tag="ACF3T5_000200"
     CDS             complement(23459..24145)
                     /gene="gpmA"
                     /locus_tag="ACF3T5_000200"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFD3630843.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24317..24898)
                     /locus_tag="ACF3T5_000201"
     CDS             complement(24317..24898)
                     /locus_tag="ACF3T5_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3630844.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25031..25714)
                     /gene="rpiA"
                     /locus_tag="ACF3T5_000202"
     CDS             complement(25031..25714)
                     /gene="rpiA"
                     /locus_tag="ACF3T5_000202"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFD3630845.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26033..26446
                     /gene="rpsL"
                     /locus_tag="ACF3T5_000203"
     CDS             26033..26446
                     /gene="rpsL"
                     /locus_tag="ACF3T5_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFD3630846.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26530..27000
                     /gene="rpsG"
                     /locus_tag="ACF3T5_000204"
     CDS             26530..27000
                     /gene="rpsG"
                     /locus_tag="ACF3T5_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFD3630847.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27094..29181
                     /gene="fusA"
                     /locus_tag="ACF3T5_000205"
     CDS             27094..29181
                     /gene="fusA"
                     /locus_tag="ACF3T5_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFD3630848.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29353..30540
                     /gene="tuf"
                     /locus_tag="ACF3T5_000206"
     CDS             29353..30540
                     /gene="tuf"
                     /locus_tag="ACF3T5_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFD3630849.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            30947..31255
                     /gene="rpsJ"
                     /locus_tag="ACF3T5_000207"
     CDS             30947..31255
                     /gene="rpsJ"
                     /locus_tag="ACF3T5_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFD3630850.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31286..31915
                     /gene="rplC"
                     /locus_tag="ACF3T5_000208"
     CDS             31286..31915
                     /gene="rplC"
                     /locus_tag="ACF3T5_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFD3630851.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            31943..32566
                     /gene="rplD"
                     /locus_tag="ACF3T5_000209"
     CDS             31943..32566
                     /gene="rplD"
                     /locus_tag="ACF3T5_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFD3630852.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32566..32856
                     /gene="rplW"
                     /locus_tag="ACF3T5_000210"
     CDS             32566..32856
                     /gene="rplW"
                     /locus_tag="ACF3T5_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFD3630853.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            32895..33728
                     /gene="rplB"
                     /locus_tag="ACF3T5_000211"
     CDS             32895..33728
                     /gene="rplB"
                     /locus_tag="ACF3T5_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFD3630854.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33771..34049
                     /gene="rpsS"
                     /locus_tag="ACF3T5_000212"
     CDS             33771..34049
                     /gene="rpsS"
                     /locus_tag="ACF3T5_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFD3630855.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34072..34419
                     /gene="rplV"
                     /locus_tag="ACF3T5_000213"
     CDS             34072..34419
                     /gene="rplV"
                     /locus_tag="ACF3T5_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFD3630856.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34433..35089
                     /gene="rpsC"
                     /locus_tag="ACF3T5_000214"
     CDS             34433..35089
                     /gene="rpsC"
                     /locus_tag="ACF3T5_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFD3630857.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35092..35526
                     /gene="rplP"
                     /locus_tag="ACF3T5_000215"
     CDS             35092..35526
                     /gene="rplP"
                     /locus_tag="ACF3T5_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFD3630858.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35516..35704
                     /gene="rpmC"
                     /locus_tag="ACF3T5_000216"
     CDS             35516..35704
                     /gene="rpmC"
                     /locus_tag="ACF3T5_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFD3630859.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35729..35995
                     /gene="rpsQ"
                     /locus_tag="ACF3T5_000217"
     CDS             35729..35995
                     /gene="rpsQ"
                     /locus_tag="ACF3T5_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFD3630860.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36053..36421
                     /gene="rplN"
                     /locus_tag="ACF3T5_000218"
     CDS             36053..36421
                     /gene="rplN"
                     /locus_tag="ACF3T5_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFD3630861.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36458..36766
                     /gene="rplX"
                     /locus_tag="ACF3T5_000219"
     CDS             36458..36766
                     /gene="rplX"
                     /locus_tag="ACF3T5_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFD3630862.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36794..37333
                     /gene="rplE"
                     /locus_tag="ACF3T5_000220"
     CDS             36794..37333
                     /gene="rplE"
                     /locus_tag="ACF3T5_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFD3630863.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37352..37537
                     /locus_tag="ACF3T5_000221"
     CDS             37352..37537
                     /locus_tag="ACF3T5_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFD3630864.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37573..37971
                     /gene="rpsH"
                     /locus_tag="ACF3T5_000222"
     CDS             37573..37971
                     /gene="rpsH"
                     /locus_tag="ACF3T5_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFD3630865.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38003..38539
                     /gene="rplF"
                     /locus_tag="ACF3T5_000223"
     CDS             38003..38539
                     /gene="rplF"
                     /locus_tag="ACF3T5_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFD3630866.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38700..39056
                     /gene="rplR"
                     /locus_tag="ACF3T5_000224"
     CDS             38700..39056
                     /gene="rplR"
                     /locus_tag="ACF3T5_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFD3630867.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39077..39577
                     /gene="rpsE"
                     /locus_tag="ACF3T5_000225"
     CDS             39077..39577
                     /gene="rpsE"
                     /locus_tag="ACF3T5_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFD3630868.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39592..39771
                     /gene="rpmD"
                     /locus_tag="ACF3T5_000226"
     CDS             39592..39771
                     /gene="rpmD"
                     /locus_tag="ACF3T5_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFD3630869.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39815..40255
                     /gene="rplO"
                     /locus_tag="ACF3T5_000227"
     CDS             39815..40255
                     /gene="rplO"
                     /locus_tag="ACF3T5_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFD3630870.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40255..41550
                     /gene="secY"
                     /locus_tag="ACF3T5_000228"
     CDS             40255..41550
                     /gene="secY"
                     /locus_tag="ACF3T5_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFD3630871.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41612..42259
                     /locus_tag="ACF3T5_000229"
     CDS             41612..42259
                     /locus_tag="ACF3T5_000229"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFD3630872.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42450..42668
                     /gene="infA"
                     /locus_tag="ACF3T5_000230"
     CDS             42450..42668
                     /gene="infA"
                     /locus_tag="ACF3T5_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFD3630873.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42701..42817
                     /gene="rpmJ"
                     /locus_tag="ACF3T5_000231"
     CDS             42701..42817
                     /gene="rpmJ"
                     /locus_tag="ACF3T5_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFD3630874.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42835..43200
                     /gene="rpsM"
                     /locus_tag="ACF3T5_000232"
     CDS             42835..43200
                     /gene="rpsM"
                     /locus_tag="ACF3T5_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFD3630875.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43228..43617
                     /gene="rpsK"
                     /locus_tag="ACF3T5_000233"
     CDS             43228..43617
                     /gene="rpsK"
                     /locus_tag="ACF3T5_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFD3630876.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43697..44635
                     /locus_tag="ACF3T5_000234"
     CDS             43697..44635
                     /locus_tag="ACF3T5_000234"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFD3630877.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44663..45043
                     /gene="rplQ"
                     /locus_tag="ACF3T5_000235"
     CDS             44663..45043
                     /gene="rplQ"
                     /locus_tag="ACF3T5_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFD3630878.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45315..46154
                     /locus_tag="ACF3T5_000236"
     CDS             45315..46154
                     /locus_tag="ACF3T5_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFD3630879.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46130..46999
                     /locus_tag="ACF3T5_000237"
     CDS             46130..46999
                     /locus_tag="ACF3T5_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFD3630880.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            46989..47786
                     /locus_tag="ACF3T5_000238"
     CDS             46989..47786
                     /locus_tag="ACF3T5_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFD3630881.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            47989..48735
                     /gene="truA"
                     /locus_tag="ACF3T5_000239"
     CDS             47989..48735
                     /gene="truA"
                     /locus_tag="ACF3T5_000239"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFD3630882.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49190..51859)
                     /locus_tag="ACF3T5_000240"
     CDS             complement(49190..51859)
                     /locus_tag="ACF3T5_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFD3630883.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        16461 a         9525 c        11304 g        14626 t
ORIGIN      
        1 attaaaaata ttatagaatc aacactcaga gaacgggttg gtttttaata gatttaataa
       61 gtactgataa tgaaatttct cctggttggg aaaacgaagt tggttaccct gtagaagaat
      121 tttttcaaga ttactttgcg gagtatgccg ataggataat gagagattac gtaacagata
      181 aaactcaaga agtcatcaat caatcccagt cattaaatga attatctgat aaggcgaaag
      241 aagaagcagt tgccattatt tcttcaggga ttagtgacta tattgatatg agtggtgaaa
      301 tcaattatgg cgataaagac aaaattgtac gcatgataaa agcttttatc aaaacttttg
      361 atgtgaaggc agatcttaag tttaatgata cttacctagt aggaaaatta attaatatcc
      421 ttgggacaga gaaagatacg gaaacgttta tttcctcttt gtctatgttg tttacagata
      481 agcatttttc aactcagctt ggtggagagg aactagtgga aatcttttaa ccattacgcc
      541 cagcattaat aaaagtatat caaaaaagta ctcctgatga tttttaagat tggtttgaga
      601 aataatgtga aaacactatt tttaatgtta attatagata gagatgaacg cgtcatatgg
      661 ttaaaagaat gaaaaagtca aagatacact gctatatttc actaaggata tagcagtgta
      721 tctttcatgt cgtttcactg gcagtttttt ataaaaggag gtgtgcattt cagttgcttt
      781 tctttggtag ttagttgaat cgtttaagaa ttaacggatg tggcagacag gtgctctgtc
      841 caaaaaccag taaaatcttt gatggttcca tctttattta gctgtgcctg ttcaaatact
      901 gttttgtttt taccgaatgg atcaattgca ataattgttc cgatccccgc acgtttggca
      961 gcaagtaatc ctgagtaagc atcttcgata acaagacaat cagccggagc taagctcagt
     1021 ttgtctgccg ctttaagaaa aatatccggt tgcggtttcc cagggaaaga gccgtcgtca
     1081 tagacaatct tgtcccagtc aaaccagcgg gctaaatcga aaatatcaaa ataaaaagca
     1141 acattctctt tgacagtagc cgtggcaatc gttaatggaa tctgttggtc agtcagctgg
     1201 tcaagagtcg cttcaagccc ttttgtcaac tgaagctcgt tttcattttg caagcaaagc
     1261 atacgataat agctttcttt ttcttctgac aatacggcga tctcagaggc ggtcaaatgc
     1321 tctgaaatga aatgtttcaa aatctccgta ttcgtccgac catgaatatt ttttaaaata
     1381 tcctcgtctg ataaaccatc ttttgtatga tcatgtatca tatgcagcca agcagcttca
     1441 tgcagatgag agtccaaaaa cattgttccg ttgaaatcaa atataatccc tttcatagtt
     1501 acgctcctat agtggtattt tcggttgaat cagcaatttg cagatcgaca tattgtgcat
     1561 agtgctcttg aacctcccgc gagatctggc gatccgtaat gagcagatcg aaatcatgca
     1621 aattataaaa cacataaaaa tcatctcgat tgaatttttt attatcaacg agtagatatt
     1681 tcttacggga attatttaga gcgatctgct gtgcttctcc ttcttcggta ctgtaagtag
     1741 agatcgcttc gttgtgaacg ccatttgaac tgataaatgc ttttgtaaac tttagtcttc
     1801 ttaaattgtc gttggtgatc ggtccgacaa atgcgccggt atttttccgg aaatcaccgc
     1861 caatcagtag aatttctggt ccatctgctt gagcaagaat atcaaaaact ggataattat
     1921 ttgtaatgat ccgaattttc ttgttcagta aatgcgtcgc caatagttcg attgttgtac
     1981 ctggtcctaa gaaaatcgtt tctccctcgt gaatcaagga ggctgctgtg gcagcaattt
     2041 cttttttttc ttgaatctgc agagtggctt tttcagtgtg agaaagttct tgatctaaag
     2101 tgtaggaaag actttgagcc cctccatgga cacgcagcag cttgcccgat ttttctaatt
     2161 catctaaatc tctgcgaacg gtcatatcag aaacatctaa ttgattgatg atttcattaa
     2221 cggtaaggat tcctttttta ttgaccattt ctacgatagt aagcaatctt tcttttttca
     2281 acattgtctt cacctctaat ttaaggataa tctatcatat aaacggacaa cagtcaatct
     2341 aattcgaaca aaagtaaaca tttagggtgg tttttatgtt tgttacaata gattttattg
     2401 ctgttttctg gtttttatga ttatttgtgt ttgtttttgt tgacaaatgt tttggatgcg
     2461 tttacaatgc aatcagttag aaagagcgaa aactgattat agatttgagg agagatgtac
     2521 atgcttaaat taccagaaga ttttattttt ggcggagcca cagcagccta tcaggttgaa
     2581 ggagccacta aggaaggtgg caaaggcgct gttgcttggg atgacttttt agaggaacaa
     2641 gggcgattca gtcccgatcc ggcaagtgat ttttatcatc aatatgcaaa ggacattgaa
     2701 ttgtgtgaac gttttggtgt aaatggtcta agattatcca ttgcttggag ccgcattttt
     2761 cctgatggag ctgggaaacc aaatccagaa ggaatcgctt tttatcatcg tgtgttcgaa
     2821 gaatgtaaga aaagaaacgt aacgccgttt gtgacgttgc atcattttga tacccctaaa
     2881 cgtttgtttg accatggaga ttttttgaat cgagaaacaa tagaagcatt tgtttcctat
     2941 gccatttttt gttttcatga attcaaagaa gtcaaggtgt ggagtacctt taatgaaatc
     3001 tatccagttg caaccaatca atatttgtta ggtgttttcc caccaggaat caaatatgat
     3061 tttacaaaga tcgtcgcctg tcttcataat atgatggttg ctcatgcccg tgtagtgaac
     3121 tatttcaaag aaaacgaact tccgggtgaa attggtgtag ttcactcatt agagacgaaa
     3181 tatgcggcaa cagacgcgcc ggaagataag catgctgcct ttttagacga tgcattgtcg
     3241 atccgtttct tattagatgc tacctattta ggctattatt caactgaaac cttgactgct
     3301 cttgatgaga tatgtgaagc aaatcaggcc agctatcatt ttcctgaaga agatttcgtt
     3361 gaattgaaaa aagcatccac gcgtaatgac tacttaggga tcaatcacta ccaatgccat
     3421 tttgtcaaag cttacgatgg agaaaatgca atccatcata acggtacggg tgaaaaaggg
     3481 acctctgttt ataaagtgaa gggaatcgga gaacgtattt ataaagaagg aattcctcga
     3541 actgattggg actggctgat ttatccagaa ggtctatatg acttgttgct gcgaattaaa
     3601 agcgattacc cacattacaa caaaatctat atcactgaga acgggatggg gtataaagat
     3661 cagtttgaag atggcattat tatggatcag ccgagaatcg actatttgag agtctatttg
     3721 gaatccttaa gcaaggcgat aacagccggg gtaaatgtga aggggtattt tttgtggtcg
     3781 cttatggatc ttttctcatg gacaaatggg tataacaaaa ggtatggctt gttttatgtt
     3841 gattttgaaa cccagaagcg ttacccaaaa gaatcagctt actggtacaa gttggtcagt
     3901 gaaaccaaaa cgattattta ataagtgata atggaggaag aaaagatgaa taaagaggaa
     3961 ttacaaatgc ttggttttga gatcgttgca tattcaggcg acgctcgcag tacattattg
     4021 aaattattga aagaagtaag acaaggaaac tttgaacatg ttgattcggc gttgaaagaa
     4081 gccgacgaaa atttaaccct tgcacataat tcgcaaacaa aaattttagc ggaagaagca
     4141 tctgggaaag agatggagat gggattcatt tttatccatg ggcaggatca tttaatgacc
     4201 actttattgc tgagagatct gattcaagac tttattgtac tgtacagaca gaatcaagga
     4261 taggagaaga acgctatgga tggaatcatt aaacaaatcg aaaaagggaa agcatttttt
     4321 gaaaaaatct cacgtaatag ttatttagga gccgttcgtg acggattttt aacagcgatg
     4381 ccagctattt tattttcaag cattttcatt ttgattgctg cggttcctga gatttttggt
     4441 taccaatggc ctgaatctgt ctccacatgg ctatggaaag tctataatta ttcgatgggt
     4501 gttgtagggt tactcgtttc agcaaccact gctcgctgct tggctgattc gatgaatcgt
     4561 cgcttaccga aaaacaaagt aatcaacacc acctctgtga tgctggcctc tgtggtaagt
     4621 ttcctgttgt taagcgtctc tccgattgaa ggtggatttt caacggaata tatggggaca
     4681 aagggactac tggcttcctt tgtcgctgca tttttaacag ttaatttata taagttttgt
     4741 gtgctgaaag atattaccat taaaatgcct aaagaagttc ctggaacgat ttcgcaaacc
     4801 ttccgagata tttttccttt ctctttttca gtatttgcgg ccgttttaat tgatttaggc
     4861 attcgacaaa tcttccaaat ctcatttgca gaagcaatca ttacggtgct gcagccgcta
     4921 tttacagcag ctgacgggta cttaggaatc gctattatct ggggagcgat ggcgctgttt
     4981 tggtttgtcg gtgttcatgg accgtcgatc gtggagccgg caattgcggc aattatctat
     5041 gccaatgtgg aaaccaatct gcaattgttc aattccggtg aacaagcttc taaagtactg
     5101 acggtgggtt tagggaattt tgtaggaact atgggaggaa ctggggcaac attggttgtt
     5161 ccttttatct tcctactctt tgctaagtct aagcagttga aggcagttgg gaaagcttcc
     5221 tttattccag taagtttcgc tgtcaacgaa ccattgctat ttgcggcacc catgattttg
     5281 aacccttatt tttttattcc atttttgtta acacctgttg taaacgtttg cctgttcaag
     5341 ttttttgtcg atgtattggg catgaacagc tttatgtacg ttttgccttg ggcaactcca
     5401 gcaccgatcg gtttggttct aggaacgggc atgggtatct tagcttttgt tctagcagcg
     5461 gttctaatcg tcgttgactt cctgatctat ctaccatttt gcaaagcata cgatgctgtc
     5521 ttagtagaga aagaagcaca aaaagcagag gtgatcgcaa ccgaggaagg tgcttctgcc
     5581 gatgtacgtg aggaagtacc tgtggcagcg agtcaagtta tcagcgatgc aaataaagaa
     5641 atcaatgtgt tggtactttg tgctggcgca gggacaagtg cgatgttagc taatgcttta
     5701 aaaaaaggtg catctgaatc gaaagcaccg atcagtgcaa tggcgggtgc ttatggttct
     5761 cattacgaaa tgctgcagga ttttgatatg gtggtcttag caccgcaagt caattcttac
     5821 tatgatgata tcaaagaaga caccgatcgc ttaggaatca agctggttgc gacaaaagga
     5881 gcagaatata tcggattaac gagagatcct gaaaaagctg ttcagtttat tctgaaccaa
     5941 tttcaataaa ggaggagcca taatgaaaat gggaatttgg ttgcgtaaag ctgttttatt
     6001 tgcagctgtg ttaggggctt tgggggctgt ttcaaaaact gttgaagcat ctgccagcga
     6061 atttattcgc ggagcagata tttcgatcct gtcagatatg gaaaaatctg gtgcaaaata
     6121 ttatgaagac gatatttcaa aagatgcctt agagatttta aaaaataatg gtgtcaacta
     6181 tgttcgactg cgtttgtggc aggatcctta taatagtaat ggagaaagtt atggagcagg
     6241 gacaaatgat ctggaaacga cgattgacct tgcaaaacga gcaaaaaatc tgggacttaa
     6301 ggtgctgctg gattttcact acagtgattt ttgggttgat cctgggaaac agaatcttcc
     6361 taaagcatgg caaggtctga cctttgaaga aatgaatacg gcattgtacg actatacaaa
     6421 gaacgttctg tcggaaatga agcagctgga cgtttatcct gatatggttc agatcggcaa
     6481 tgaactgaat agcggaatgc tctggcctta tggcaagtct tggggagaag gcgggggaga
     6541 atttgatcga ttggctgctt ttttgaagtc tggtatccaa gctgttcgag acactcaacc
     6601 taaaacaact ccagtaatgc tccacttagc tgatggtggc gatacaggag catttacttg
     6661 gtggtttgat gaaatcacga gtcgaggtgt gtcatttgat ttaattggag tgtcgtatta
     6721 tccgtattgg cacggttcaa tgaatgaact tcaaaccaat atgaatgcga tcagccaacg
     6781 gtatggtaaa gaagtgatcg tagttgaaac agcttatggg catacaacag cgaatgccga
     6841 tactatgcca aatgcttttg gtgaagccga agcagctgcg gggggatatg aaccaacacc
     6901 gacaggacaa gcagagtatt tgctggatct tgccgatcgg attcaggcag taccgaataa
     6961 tcgcggggcg ggcttcttct attgggaacc tctttggtac aacggaaatg tgagttgggc
     7021 aacacaagca ggtatgaatt atctcagtgt tcaatccact atgggaaacg aatgggacaa
     7081 ccaagcgatg tttgattttg ctggaaacgc cttgccaagc ttgcgtgcgt ttaaacaagc
     7141 aggggcgcaa ataaatctag ttaaaaatgc aagtttcgaa agcgatggac caacgaccag
     7201 tccttccaat tggcaaacct ggtttcaaca aggaaccgct aagggtacga tcaaaacgga
     7261 agcgggcact ttttttgggg actacaaact aactttttgg caggatcaag catacgaggc
     7321 atcagcttat caaaatgtta gtggcataag caaaggaact tacacattga gtgcctatgt
     7381 aatgtctggg ggagagtttg atactaatca gttatatatc aaaaattatg gtggagcgga
     7441 actaaatact gcggttgtta aaacaagtga atggacaaaa attacaatca aagacattcc
     7501 aattacaaca ggtgtttgtg aaattggggt ttatagcaag gcttcaggaa atgcttggac
     7561 gaacgttgat tttgtttcat tggtgaaaca atagactgcg gatcgtttcg attcaagatc
     7621 ggaggttccc ttcagccaca tcgttagcgt agaaggcagg aatcgaacct gcacctcgcc
     7681 tttgccaaaa ataaccagaa acgatttcca gtcattttta ccaaaacgag taaagcacca
     7741 gacttcatct acactttgta ttatgctaaa gagagttcaa cgatttcgta agaagcaatg
     7801 aaaaaattgc aaaaagttgt gcagataaca taaagtttgt ttaagaaagg gtaaccttag
     7861 aattccgtcg tgaatatgat ttgagcactt agatggatag aaaaaagttc tcggaaaaca
     7921 gttcgttttt cgagaacttc tttttatgat aagaatgaaa caaattaact tatattatag
     7981 tctcttttgg tagggaacca tcttattttt aatgaaaatc aagcatttta gctattgaag
     8041 aatgttgatt tatagggatt ccccctccat attatgttgg ctctttattc ccactccact
     8101 gttgctggtg gtttagaagt gatgtcatac acgatacggt tgacgtgggc tacttcgttt
     8161 acgatacgta cagagatttt ttgcaagact tcccatggga tacgggcaaa gtcagcggtc
     8221 attccgtcga ttgaagttac agcacggata ccgactgtgt agtcgtaagt acgtccgtca
     8281 cccataacac cgacagagcg gatccctgga agaacagtga aatattgcca gatatcacgg
     8341 tcaagtccag cagctgcgat ttcttcacgc aagatagcat cagattcgcg tacgatttct
     8401 agcttgtctt cagtgatttc gccaagaaca cgaataccaa gaccaggacc agggaatggc
     8461 tgacgccaaa cgattgaatc aggcatgcct aactgagtcc ctaattcacg gacttcgtct
     8521 ttgaataatg tgtttaatgg ttcgatcaat tcaaattgca tatcttctgg cagtccacct
     8581 acattatggt gagatttgat cgtctgtgct gtttctgtac cagattcgat aacgtcagta
     8641 tacaacgttc cttgtgctag gaaagagacg ccttcttcac cagcaagttt tgttgcttcg
     8701 tcatcaaaaa catatacgaa ttcattccca atgattttac gtttttgttc tgggtcagat
     8761 acacctttca acttgtctaa gaagcgatct cttgcattga ctttgatgat gttcaagccg
     8821 aattttccgc ctagactttc cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg
     8881 tcaacaaaga tagatgtcaa ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca
     8941 ctagaatcaa cgccacctga aagaccaagc aagacttttt tgtcaccaac ttgttcacgg
     9001 attttttcta cttgcatatc gataaagtta tccattgtcc agttaccttc acagtgacaa
     9061 acatcaaagg caaagtggcg cagcaattcg attccgtatt cggaatgacg tacttctgca
     9121 tggaattgga taccataaaa attacgtgtt gtgtcttgga tagaagcaat cggacaatct
     9181 ttgcttgttc cgactgtttc aaagccttca ggaactttag ttaccaagtc accatggctc
     9241 atccatactg tttgttgttt tggtgtttct ttaaacaaga cagcttgatc gcttgttact
     9301 tctaacaagg ctttcccata ttcgcggttt ttagcaggct ctacggaacc gccaagtttg
     9361 tacgtgatca attgcatacc atagcaaatc cctaatacag gaatacctaa attgaagatc
     9421 tcaggatcga tcccaaatgc attctcatca tatacactgt ttggtccgcc agaaagaatg
     9481 attccctttg gattcatcgc ttttacttct tctgcagtaa tgcggtggct caataattcg
     9541 gaaaagactc caagatcacg aatacgtctt gtgatcaatt ggttatactg actaccgtag
     9601 tctaatacga taatcttttc aacgttcgtc atatcggcaa cgtttgtcaa atttattacc
     9661 cctatccctt taaattttat ctcaaaatag tcaaactatt ttaaaatgct aatattttcg
     9721 catgaaaatc gaatcgatga tatgattttc agttttatgg agaattatat cggctcttcc
     9781 tcgtgtaggc aagatatatt cctgtaaatt tttcaaattc acatttttcc atacatcttt
     9841 tgccatttta aaggcttctt cacgatctcc gatagcaaaa cgataataat aattctctgg
     9901 attttggaaa gccgtatcca gtaacgcacc aaaacgatcc aaataccatt tttcgatcag
     9961 cacgggatcg gcatcgacat agacagaaaa atcaaaaaag tcgctgacgt agatttgctg
    10021 attcgcaggt aattgaagcg tattgatccc ctcaacgatc aagatatcag gctgctggat
    10081 caattcgtac ttgtctggaa tgatatcata cacatcatgt gagtaaacag gtacttttat
    10141 ctcttcctga ccagatttta cacggttcaa gaaatcgatc agcatctcca tatcataact
    10201 ttcaggaaat cctttacggt ccatgattcc acgctctttt aatatacgat ttggataaag
    10261 aaatccgtca gtcgtgatca gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt
    10321 ctgcagcagt ctggctgttg tactttttcc aacggctaca cttccggcga tcccgataat
    10381 aaaaggcggt acttctacgt atttatgaag aaataaacct ttactcaaga acaaagattc
    10441 aaattctttc atataaagat aaatcagatg agcaagaggt acatagatat ctcgaacatc
    10501 ctgcatggaa atgcggtcat tcaaactttt gatattatct agttcatctt gtgtcaaagg
    10561 tgcttgccca ttacgataaa attctcgcca ttcttctctt gccacacggt agtagttcat
    10621 tggatcattc atattagcct cctgcgttct cccatcaatg gaactatttt aacacaaaat
    10681 aaacgcaaac cccaccactt aagagagatt gccgatcttt ttgtataatc tttataatag
    10741 aaagaaacgg agtcttattc ttgacttgcg cacggataca taaaaggata gactgttgtc
    10801 atgaaagaaa tgagaaggat ggaagacatg acaaatcatt actattctga acgcccagat
    10861 acggcgcacg attttgaaca atgggctttt gagttaaaag gcagaacctt tcaatttgta
    10921 acagattctg gtgtattttc acgtgaaaca gtggattatg gttcacgtgt cctgattgat
    10981 gctttcgatt gggaagagtt acctgatgaa ggaaagatct tagatgtggg atgcgggtat
    11041 ggaccgattg ggttagcttt ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt
    11101 aatgaacgag cggtaagtct agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt
    11161 gatatccatc aatcgaatat ctatgaagca gttcatgaag aaacctatgc agctatcgta
    11221 agcaatccgc cgatccgtgc tggaaaaaaa gttgtacatg aaattttgac aggcgcatat
    11281 cctcggttga agaagggcgg tacactgaca attgtgatcc agaagaaaca aggagcgccg
    11341 agtgcgcaaa agaaaatgga agaaacattt ggtaatgcag aaatcgtcac aaaagataaa
    11401 ggatattata tcatcaaaag tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa
    11461 agagtggaac tgggcgtgca tgtacgggta cgtatcagtt ctttttttgt tgagaagttt
    11521 ttaaaaacaa agaactattt tttcttcaaa aagaaatagt aggaacagag tcgttgtttt
    11581 ttacgataaa ataaataaga tacagaactt gtatagaagg ctgccaagtc ggtagataaa
    11641 ggaggttttt atcaaaaaac atactttcct tttttctcta ttcttttctt gggggttttg
    11701 gtacactgat agggaattta gatagaaggg agccaatagg atgaagctga ccatcagaga
    11761 tatcgcagaa atggcaggcg tatcagtaac aactgtctca cagattttga ataacaaagg
    11821 aagcagattt agtgataaga caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta
    11881 taagcccgat tatttcgctt caaatgtgat caatcgacat tcaaagacca tcggaatgat
    11941 cgtccctgat gtcactgatt tcttcttctc caaactgatc gaaggtgtag agagctattt
    12001 gaatcctttg ggctatgtga tcatgttatg caattctcgt catagccaag aaaatgagat
    12061 caagtattta caagagcttt cccaccgctc agtggacgga atcttgctgg ccacaccaaa
    12121 tattttacct gagaaatatg cattagacag cggtttttat caaaaaatgc caatcatttt
    12181 aatcgatcgc ggattgaatc gtcgggataa cggacggctt atcgtaaagg aatatgaggg
    12241 cgcctatcaa gctgtttcgc tgtttattga aaatggccat acgaagatcg gcatgctgaa
    12301 ggaaaccact ggatactatc aattagaaga acgtttcaac ggatatcgtc atgcgctgaa
    12361 agatcatggc ctgcctttta atgggaaatt tgttgaacat ggagagttga ctgtccaagg
    12421 tggatatact gcatcaaaaa aattactcaa acataaagat atcacggcta ttttttgcgg
    12481 gaatgatgcc atggcgatcg gatgctatca ggcgatcgat gaattgggca aaaaaatccc
    12541 agaagatatc tcagtcatag gattcgatgg attaaagctg tcagaataca tgattccgca
    12601 attgacgaca gtgcagcagc caagttttga tattggattt tatgctgcac gtttcctgat
    12661 cgatacgatc gaatttcctc aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat
    12721 tattcgtgag agcgtaaaag tattgacaaa gggctaactc ggcggtatat tgcttatgta
    12781 agcatttaca aaggctagtt agtgcttttt ttatttttag taaaccagtt tactaaagtg
    12841 gtttaccttt cctaaaaata atgtataata ccgaatgaag acaaggagga tcaggcaatg
    12901 cgaatggtgg atctgattga aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc
    12961 gagtacatcg tcacgaacta tacgaatgga aagattccag attatcaagt cagtgcatta
    13021 ttgatggcaa ttttttatca agacatgaca aatgaagaaa tcacgaactt aacattggcg
    13081 attgctaatt ctggtgatgt catcgatttg agttctcttg aaggaataaa ggtagataag
    13141 cattcgacag gtggcgttgg tgacacgacc acattgattt tagcccccct agtagctagt
    13201 gtcggagtga cagtggctaa aatgtctgga cgcggtctag gttacacagg tggtacacta
    13261 gacaagctag aagcaattcc cggatttcag atcgagcttt cggatgaaga atttgtacgc
    13321 attgttaatg aaagtaaagt agcggtaatt ggtcaatcag gaaacttggc accggcggac
    13381 aaaaaattgt atgccttgcg tgacgtcaca gcgaccgttg attctcttcc gctgattgcc
    13441 agttcgatta tgagtaaaaa aatcgcggca ggagcagatg caatcgtttt ggatgtgacc
    13501 actggagacg gtgctttcat gaagaacatc gaagatgcaa gacgtctggc aaagaccatg
    13561 acgagcattg ggaaactggc taaccgagaa acagtggcgg ttatctcaga tatgtcagaa
    13621 cctcttggcg aagctatcgg aaatagtcta gaagttgtcg aggcaatcga aacattacaa
    13681 ggaaatggtc cagaagatct agtagaaatg tgctacgcct taggaagcca aatggttgtt
    13741 cttgctggca aagctaagac aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa
    13801 tcaggtaaag cgttagcaaa attcaaagaa atgatccaaa atcaaggggg agacccagcg
    13861 atcgtggaac atccagaacg aattttgact gcacgctaca caatggagct tcctgctaaa
    13921 caaagcggag ttgtgtcaaa aatcgtggca aatgaattag gaattgctgc aatgatgcta
    13981 ggtgctgggc gtaaaaccaa agaagaagat attgatcacg ctgttggatt gaaactgcat
    14041 aaaaaaattg gtgacactgt gacaaaaggt gagtcactat tgacgatcta tagtaacgac
    14101 gaagaaatca cttctgtcat tgaattatta tataaaaata ttgaaattgg tgaatcggca
    14161 atgaagccaa cgttgattca tgacatcatc acagaataaa ggaaaaaagc ggactttacc
    14221 gttttaaaga aaaggggaaa agaaatggaa ctaaaccgaa tgatcgacca tactattttg
    14281 aaagcagacg catctaaaga agctgtgatg caaattatcg aagaagcaaa aaaataccat
    14341 ttttattctg tctgcatcaa tccagcatgg gttgcgctag cgaaagaaca attacaaggt
    14401 gagccagtag ccgtatgtac agtaatcggg tttcctctag gagcaaacac acccgagaca
    14461 aaagcatttg aaacaacaga tgccatcaat aacggtgcag atgaagtcga tatggtcatc
    14521 aatatcggtg ctttgaaatc aaaggatgat caacaagtcc aaaaagatat cgaagcagtt
    14581 gttgaagcag caaaagatcg tgcgcttgtc aaagttatta ttgaaacatc gttattagat
    14641 agagaagaaa tcatccgagc ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa
    14701 acatcaactg gtttctcaac aggcggagca aaggtagagg atgtccgttt gatgcgtgaa
    14761 actgtaggac cagaaatggg tgtcaaagct tccggcggta tccataacga agaagaagca
    14821 atggcaatga tcgaagctgg tgctacaaga atcggaacaa gcgcaggagt agcaatcgta
    14881 tctggttcaa ctggtgaagg ctattaattt tttcaaagga gaagctcatg gcagaactca
    14941 aacaagaatg gatcaacaca gctattgaag cgttagataa agcctatgtt ccttattccc
    15001 attttccggt aggcgcttgt ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca
    15061 ttgagaatgc atcatttgga ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag
    15121 tatctgaagg agaaaaaaca tttacgcatc tagtcgtagc ggggcacacc cccgatccta
    15181 tctctccttg tggtgcatgt cgacaagtaa tggcagaatt ctgttcccct gatatgccgg
    15241 ttacgctagt aggagacaat ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt
    15301 atgcatttac tgaaaaagat ctataactca tgttcattca gtcagctgac tgttgaaaaa
    15361 taaaacaatt attttaggag gctttacaga aatgaaaaaa gcaaaattat ttggtttagg
    15421 tgctgtcgca ctagcagcag ggttattttt aggtgcatgc ggcaacaacg gctctactga
    15481 ttcatccggt ggcaaaagct catcagacac aacaactgca gcattgatca cagacacagg
    15541 tggtgtagac gaccgttcat tcaaccagtc agcttgggaa ggactagaaa aatgggggaa
    15601 agatcaagga ctttcacgag gaaatgatgg cttccaatat ttccaatctt caaatgaatc
    15661 agattacatc ccaaatattg accaagcatt gaatgctgga ttcaaaacga tttttggtat
    15721 tggttataaa ctaaaaccag cgatccaaga acaagcaaca aacaatacag gtacgaactt
    15781 tgttattatc gatgatgtca tcgatggatt agataacgtt gtatctgcaa ctttcaaaga
    15841 caacgaagct tcttatcttg caggtgttgc agcagcttat acgactgaaa caaacgtagt
    15901 cggctttatc ggcggtgtaa aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc
    15961 cggtgttgat gcaggtgcga aagaattagg aaaagaaatc aaagtattga atcaatatgc
    16021 tggtgacttc tctgctccag ataaaggacg ttcaattgct caaggtatgt atgcacaaaa
    16081 cgcagatatc atcttccatg cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa
    16141 atcattgaac gaatcaggcg acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc
    16201 agacgaaggc gaatacacat taaacggcga aaagaaaaac ttcacattga catctacact
    16261 gaaagcagtt ggaacagtag tagaagattt ggcacaaaaa tcagcggacg gtaaattccc
    16321 tggcggcgaa cacactgttt atggattgaa agaagacggt gttggtctaa cagaaggaca
    16381 actttctgac gaagcgaaaa aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga
    16441 cgtaaaagtt ccagaaactc ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca
    16501 catgtttgac aaggctatgg tttgattaga acaagaattg ttcatttatc caagctgcct
    16561 tgtgacagtt tggataaggg aacaactccc tgaagtaatg tttgtttcaa aagaagaacg
    16621 ctcactaagc gtgaatctta gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa
    16681 aaaaggtggt tcaagaaaat attgtcatag aaatgcagaa tattaccaaa gaatttggaa
    16741 cattcaaggc aaatgacaac atcaatttac aagtaaaggc aggagaaatt catgcgttgc
    16801 ttggagaaaa cggtgctggc aaatctacat tgatgaacgt gctttccgga ttattagagc
    16861 cgacatcagg gaaaattttg atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag
    16921 ccaaccaatt agggattggg atggtccatc agcactttat gcttgttgat gcctttactg
    16981 taacagaaaa catcgtgttg ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa
    17041 aagcacgaaa agagatccaa aaagtttctg aacagtatgg attgtcagtc aacccagatg
    17101 cttacgttcg tgatatttca gttggaatgg aacaacgggt agaaatttta aaaacacttt
    17161 accgaggagc agatgtactg atttttgatg agccgacagc tgtattgacc cctcaagaaa
    17221 ttgatgaatt aatcgtgatc atgaaggaat tagtcaaaga aggcaagtcg atcattttga
    17281 ttacgcataa gttagatgaa atcaaagcag tagctgaccg ctgtacagtt atccgccgtg
    17341 gaaaaggaat cggtacagtc aacgttaaag acgtaacctc acaacaatta gccgatatga
    17401 tggttggaag aacggtttca ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg
    17461 ttttgtctat tgaaaatcta gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact
    17521 tgaacttaga ggttcgtgct ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc
    17581 agtcggagtt gatccaagct ttgactggtt tgcgaaaggc agaaagcgga catatcaagc
    17641 taaaagggga agacatcact aataaaaaac cccgaaaaat cactgaacat ggtgtaggac
    17701 acgtgccaga agaccgtcat aaatacgggt tagtcctaga tatgacatta tctgaaaata
    17761 tcgcactaca aacctatcat caaaaacctt acagtaaaaa cggcatgctg aattattcag
    17821 taatgaatga acatgccaga gaattgatcg aagaatatga tgttcgaaca acgaatgaac
    17881 ttgttcccgc aaaagcttta tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag
    17941 tcgaccgaga tcctgatctg ttgattgttg caaatccaac tcgtggactg gatgtaggtg
    18001 cgatcgaatt tattcataaa cgtctgatcg aacaaaggga caaatacaaa gcagtgttac
    18061 tgatcagttt cgaattagaa gaaattttaa atgtttcgga tcgtattgct gttatccatg
    18121 aaggagaaat tgtcggtatc gttgatccga aagaaacatc tgaaaatgaa ttaggtctat
    18181 tgatggcggg ctatacctta gaagaagcac gaaaagagtt agaaaagggg gctggcgcac
    18241 atgaatgatc gcagcgaaaa attccgtaat ttattagtgc ctgtactttc agttgtttta
    18301 ggattgatct taggcgcgat attgatggct gcttttggtt ttcagcccat ccaaggttat
    18361 tcttctatgc tagatgcagc ccttggttcg caaagaggga taggtgaaac gttaaggcaa
    18421 gcaacgccgt tgatttttac agcattaggt ttttctgtag ctaactcagc tgggtttttc
    18481 aatattgggc tttctggaca agcactttgc gggtggatcg tcagtgtctg ggtggcactt
    18541 gcgtttccag atctgccaaa agttgttttg cttccgttat gcgtgatctt aggtgcctta
    18601 gctggtgcga tggcagcggc tgttccaggt ttacttcgag cattttttgg aacaagtgaa
    18661 gtgatcgtaa caatcatgat gaactatatc ttattatacg taagcacatt tgctttacat
    18721 gaagtgatgc ctgaaagcta tcgctcaagt ttggactctt ccaatatgat cagtcaaaat
    18781 gcctcactgc ggttaccatg gttaacacaa gcttttggcg gctctcgcgt aaactcagga
    18841 ttatttttag caatcatcgc gcttattttc gtttggctgc tgatgaagaa aacgacttta
    18901 ggatttgaaa tccgttctgt tggattgaat ccttttgctt ctgagtatgc aggaatgagt
    18961 agtaaacgta cgatcgtttt gtcgatggtc atttctggcg ctttggcagg gcttggcggt
    19021 gtggttgaag gattaggaac gtatcaaaac ttctttgttc aaacgacttc gctatctatt
    19081 ggatttgacg gaatggctgt ttccttgctt ggttctggat cagcaatcgg tattctttta
    19141 tcagcgatct tgttcagtat tttgaagatc ggtggacttg gcatgcagac aggagcaggt
    19201 gttccgtttg agatcgtcaa tgtatcgatt gctttgatta tcttctttgt cgggatcaac
    19261 ttcctgattc gtttcgtcat ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt
    19321 gcgacaatag aatcgaaacc aacgaatcaa aaccatgaag ggggcgaact ataatgtcga
    19381 ctatcgaatt aattgccttg atccttactt ctacactggt ttattcaaca ccgctaattc
    19441 taacgtcact aggcggaact ttttctgaac gaagcgggat cgtaaacgta gggctagagg
    19501 gaatcatggt catgggtgct tttagttcca tcgttttcaa cttgacgatg agtccatcac
    19561 ttggttctat gacaccatgg atcgctattt tagtgggtgg cgtcgtaggt atcctatttt
    19621 ctctgattca cgcagtagca acagtcagtt tgcgtgctga ccatattatc agcgggacag
    19681 taattaatct gatggcacct gctttaggtg ttttcttgat caaagcatgg tatggtaaag
    19741 gtcaaactga taacatcacg cagaatctgg ggtatttttc tttcccagga ttagcagata
    19801 ttccggtcat tggaccaatt ttctttaaaa atacattctt acctgcttat tgtgctattt
    19861 tgattgctgt cctagcttgg gtgatcatct tcaagacgaa attcggcttg cgcctccgtt
    19921 cagttggtga aaatcctcaa gctgccgaca cattaggtat caatgtatat gctatgcgtt
    19981 attcaggcgt actgatttct ggcttgttag gcggtatggg cggtgctgtt tttgctcagt
    20041 cgatttccgg taacttttca gctggtacga tcgttggaca agggttcatc tccatggctg
    20101 ctatgatctt tggtaagtgg aatcctttag gggcaatggg tgcctcattg ttctttggtt
    20161 ttgctcaaag cttgagtatc attggcgcac aattaccagt tatttcttcg attccgccag
    20221 tattgctgca aattgctcca tatttgctga caatcattgt gctggttgtt ttcttaggga
    20281 aagcttctgg tccaaaagca aacgggaaga actatatcaa atcaaaataa tcttttagcg
    20341 aataaaaagg gtgggttacg ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa
    20401 taaaaataaa aagaaagaag gaataatcat gtttaaacgc gtacatctga tcgtcatgga
    20461 ttcagtagga attggagaag caccagatgc cgataagttc ggtgatgttg gttctgatac
    20521 attaggacac attgcgaaag aagcaggatt gaacgttcca catttagagc agcttggttt
    20581 aggaacgatc cgtcctcttg aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac
    20641 gaaattagaa gaagtttctg tagggaaaga tacgatgaca ggccactggg aaatcatggg
    20701 attgaatatc aaaactccat ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa
    20761 acaaatcgaa gaattttctg gccgtaaagt cgtatgtaac aaaccttata gcggaacagc
    20821 tgttattgac gattacggtg aacaccaaat gaaaacaggc gatttaattg tatatacttc
    20881 tgccgatcct gtccttcaga tcgcggcaca tgaagaaatc attcctttag aagaattata
    20941 tcggatttgc caattcgttc gtgatataac aaaagatgat ccgtatatga ttggccgaat
    21001 cattgcacgt ccgtacgtgg gtgaaccagg agacttcaca cgaacaagca atcgccatga
    21061 ttatgcgttg aatccattcg gacacacagt ccttgattct ttgaaagaag caggaaagga
    21121 cgtgattgct gttggaaaga tcaacgatat tttcaacgga caaggaatca ctgaatctgt
    21181 ccgcacgaaa agcaatatgg atggcgttga ccagctgctg aaagttatga aaaacgattt
    21241 ttcaggaatc agctttacca atttagtaga ttttgatgca ttatacggtc atcgtcggga
    21301 tgtagtcggc tatgcgcatg cgattgaaga atttgactta cgaattccag aattgcttga
    21361 agcgatggaa gaagacgact tgctaatgat tacagccgat cacggaaacg atccgacgtt
    21421 tactggtacc gatcatacgc gagaatatgt accgttattg gtttacagta aaaaaatgaa
    21481 agaacaaggg gcattgcctc aaggatatta ttcagatatt tcagcaacga ttgctgagaa
    21541 ctttagtgtt tcttcgactg aaaatggcga aagcttcttg aaattattaa aataatcttt
    21601 ttaaatagga gaaagcaaat gaccaagtta agcgagatgt tacaagaaac gacccagttt
    21661 atccttgata aaggagtaaa agaaattgat tttggtttga ttctaggttc aggattaggt
    21721 gaattagcag atgaaatcga agatgctgta atgatttctt atgatgagat cccgttcttt
    21781 cctgtttcga cagtagttgg gcatgcagga aagctagttt atgggtctct ggcaggcaag
    21841 aaagtgttag ccatgcaagg gagattccac tactatgaag gtcattccat gcaaacagtg
    21901 acctatccag tacgtgtgat ggccggacta ggggcccatt cagtcattgt gactaatgca
    21961 tgtggtggtg tcaacgaatc atttgtacca ggagatttga tgctgattac cgatcatatc
    22021 aattttactg gtgaaaatcc tttgatcggt ccgaatgaag atgcaatggg ccctagattt
    22081 ccagatatga gtcaagctta tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa
    22141 aaactaggtt tggatcttaa agaaggcgta tatatgggct attctggacc aacttatgaa
    22201 acgcctgcgg agatccgaat gtctcggaca ataggagccg atgcagtagg gatgtcgact
    22261 gttccagaag tcatcgtagc agctcacagc ggattaaaag tgttaggaat ctcttgtatt
    22321 accaacttag cagcaggtat gcaagcgaat ctgagccatg aagaagttgt agaaacaact
    22381 cagagagtca aacaatcttt taaagcattg gttaaagaaa cattgacatt attgtaatag
    22441 gaggaacaat aaaatgagtg tgcatattga agcgaaagaa ggcgagatcg ccgataagat
    22501 tttacttccg ggagatcctt tgcgagctaa atatattgcg gagacttttt tggaagatcc
    22561 agtttgttac aatcaggtac gcggtatgtt aggctttacc ggcaaatata aaggagaacg
    22621 tatttctgtt caaggaacag gaatgggaat gccatcagcc accatctatg cccatgaatt
    22681 gatccagtcg tatggtgtaa agaaattgat tcgagtaggt acatgcggtg cattgtcaaa
    22741 agacgtccat gttcgtgatt tagtactagc acaaggtgca gcgaccagtt cttcgatgat
    22801 cgaaaaaaat ttccaagcct ttcattttcc tccaatcagt gatttcaatc tattactgaa
    22861 agcatatgag atagctaaag aaaagggcta tacggttcat gtaggaaatg ttctttcgga
    22921 agattctttc tacaaagatg atttgacaga gacgttccaa ttagctgaac ttggcgtttt
    22981 aggagtggaa atggaagcag cggctcttta ttacttaggc gcgaaatacc atgtgcagac
    23041 attaagtttg atgaccgtta gcgaccattt gatcaccggt gaagaaacaa cagctgcaga
    23101 acgtcaatct acgttcaatg aaatgatcga agtaggattg gaaacagcga tagcagaata
    23161 agaaggaaat gaaaaagctg ccctgcatta agtaataaga acatccaagc aaaaatagct
    23221 cctcatgttt ttgacttggc tggacttatt attgcaaatg taagcttttg aacaccgttt
    23281 accaagaggt tgtgaatctg ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca
    23341 gcttttcatg tttgatgaat attgtgactt gtgaccgaga agttggcagg agaaaaccgt
    23401 ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc ccttttttat agtgtttttt
    23461 ataggtagta ttttttagca actgttaagt catcatttaa ttcataaaca agtggttgac
    23521 ctgttggaat ttcaagatcc atgatgtctt cgtcagagat accttcgata tgtttagcta
    23581 gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg
    23641 gagcaatttc atcttgccag aatggcaaag cgcgttctaa tgtcactttc aagttttctc
    23701 caccaggaat atcgcgtttg tctaacattg cgtaacgacg gtcatttgct gcagaacctt
    23761 cgtcagtttc ttccattaat ggaggaagag tatcgtaaga acgacgccag atgtgcactt
    23821 gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa accttgtaat ttaccatagt
    23881 gacgttcgtt taagcgccat gatttgattt gtggtaccca aagttgatct gaatattcta
    23941 agatcaagtt acatgttttg attgcacgag ttaaaacaga agtgtaagca acatcaaatt
    24001 cgattccagc ttctttgatt ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg
    24061 ctagatttac atcagcccaa ccagtaaatt ggttcaatgc attccattca ctaagaccgt
    24121 gacgagaaaa aactaatttt ggcatgttta gctctccttt taagataggt ttatatttct
    24181 gaaattttca gttctgtttt attgtacaat tttttggaag agatttccat cgaaaaatta
    24241 gataagattc tatgagaaaa caagtagaga atataaaaaa acgaacagtg cccacgcaac
    24301 tgttcgtttt taggtattag atgcataatt gctcgatttc tttttcaggt aaattgatat
    24361 atttataaca tgttccaact gaaacaccgc attttgaaga aataaattgg atcgtttctt
    24421 ttttttcata atagagacgt cggatttttt tgatcgtttt tgcatcgatt tttggtcttc
    24481 cgccgatttt tcctttttta cgagcttcat ctaatccaac taatgtacgt tctttgatca
    24541 atgcgcactc catgtttgct aggttttcca ttaattggaa atagattttt cccatttcgc
    24601 gcgtatcgat tccttcatca agactaatca agtgcagccc gcgatctttg aataattgcg
    24661 tgagttctgt taattgccgc gtagatttgc ctaatcgatg aagttggcaa atgactagtg
    24721 catcgccttg ttgcatgtca gcgatcactg actccaaatg aacagcagta gaatcatttt
    24781 gttccacttc atatgtctca tggaaaattt cttgacaacc aaagtcagat aattgatcaa
    24841 cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc ataaccaata attttcatgg
    24901 tgattccttt ctagcagtga ttttctaact ggatataaca gtaaaaatgt agcataaagt
    24961 acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct ttttttattg gatgatcaag
    25021 cagtttttct ttatttacga cggattgcgt caagaatctc aacgcctttt tctgagccga
    25081 caactacttt agaaaccatg tttaggaaaa gcccatgttc tactacccca acaagttcat
    25141 ctaaataggt agctaagctt tctggctgtt cgatgacttc tagatgcaga tcaatgatgt
    25201 aatgaccacc atctgtggtt aagacttctc cgttttcgtc tgtacgcaag acgggttgga
    25261 atcttttttc ttcaaaaata tgaagtagct gttgggagcc ataagggatc acttcaactg
    25321 ggagtgggaa cttcccaagt ttatcgacta atttagaaga atcaacgatc caaatcgttt
    25381 cttttgagta cgtagcgact attttctcga aaagcaaagc agcgccgccg cctttgatcc
    25441 cttgaaaatc agcactgatt tcatcagcac cgtctattgt caaatcgact acggggactt
    25501 catcaatact ctttaacggt attcctaatt tttccgcttg ttcttttgtc gcatttgaag
    25561 tagtaactcc tgtgatacgt aaaccttctt cacgcatacg gcgaccaatc tcttctacca
    25621 tataataggc ggttgaaccg gtcccgagac ctacgatcat cccgtccttt acgaatttcg
    25681 cggcttcaat gccagctaat tgttttaaat tcatctttgc gtccaccttt ttttagtttg
    25741 agcatcgtgc tttcctcact attttagcgt gatttacggt aaaaagatac cattcttttt
    25801 tattccaata aagctttttc aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg
    25861 tgatattcta acaaaggtgc tttatctaca ggtttctgtg aagaaacgtg ctgactgttt
    25921 atcaaagcat atttgataag gatgcagaca ttgcatttat tttttatcac aaaaaaagaa
    25981 ccacctggat gtgtggaact agacgcaaat cgtgggaagg aggaaacaag gaatgcctac
    26041 aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc
    26101 tttgaacaaa ggatataaca gctttaagaa aactcaaact aacgtaaact cacctcaaaa
    26161 acgtggggta gcaactcgtg tgggtacaat gacacctaaa aaaccgaact cagctttacg
    26221 taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt actgcttata tcccagggat
    26281 tggtcataac ttacaagaac acagtgttgt attattacgc ggtggtcgtg taaaagactt
    26341 accaggggta cgttaccata tcgttcgcgg tgctttagat actgctggtg ttaacgaccg
    26401 taaacaaagc cgttctaaat acggaactaa aaaacctaaa gcttaatttt aattattaaa
    26461 caaatgaaac ttgcttctac tatatagaag taattactat gaacaatatt tcggaaggag
    26521 gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg atgttttacc agatcctatt
    26581 tataactcaa aattagtaac tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggg
    26641 attgcagcta acattatcta taatgccttt gacttgatca aagaatctac aggtaacgat
    26701 ccattagaag tgtttgaaca agcaatgaaa aacgttcaac ctgttcttga agttaaagca
    26761 cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag ttcgccctga acgccgcgtt
    26821 actctaggtc ttcgttgggt agtaaactac gctcgcttgc gcggtgaaca cacaatggaa
    26881 gaacgcttag caaaagaaat catggatgct gcaaacaaca caggtgcttc tgttaaaaaa
    26941 cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa
    27001 gattctctct gtttggtcgg cttttataag tcgacctcag atagtgtcct tatttttagc
    27061 aactagatag taaagagagg agaaacaagg agaatgacca gagaattttc tttacaaaac
    27121 actcgtaata tcggtatcat ggcccacatc gatgctggta aaacgactgc tactgaacgt
    27181 atcttgtact atactggtaa aatccataaa attggtgaaa ctcatgaagg agcttcacaa
    27241 atggactgga tggaacaaga acaagaacgt ggtattacga tcacttctgc tgctactaca
    27301 gcacaatgga agggtcaccg aatcaacatc atcgacacac caggacacgt agacttcaca
    27361 gttgaagttg aacgttcatt gcgtgtactt gatggtgctg taactgttct tgatgcacaa
    27421 tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag caacaactta tggtgtacct
    27481 cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg acttcttata ttcagtatca
    27541 actttacatg accgtttgca agcaaacgct cacccaatcc aattaccaat tggtgcggaa
    27601 gacgacttca caggaattat cgaccttgta acaatgaagg ctgaaatgta tacaaacgat
    27661 cttggaacag agatcgaaga aacagaaatt cctgaagaat accgcgaatt agctgaagaa
    27721 tggcgcgaaa aattggttga agctgtagct gaaactgatg aagaacttac tttgaaatac
    27781 ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt
    27841 aacgtagaat tctacccagt actatgtggt tcagcattta agaacaaagg ggttcaatta
    27901 ctattggacg ctgttcttga ttacttgcca tcaccgttag atatcccagc tatcaaaggg
    27961 attgacccta aaactgatga agaagtagaa cgtcctgcag acgacagcgc tcctttctca
    28021 gctttagcat ttaaagttat gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg
    28081 tactctggtg ttttacaatc aggttcatac gtacaaaacg caacaaaagg caaacgtgaa
    28141 cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt cagaaatctc tgaagtttac
    28201 gctggagata tcgctgctgc tgttggtttg aaagacacaa ctactgggga cacactatgt
    28261 gatgaaaaga gtctagttat tcttgaatcc atggaattcc cagaaccagt tatccaagtc
    28321 gctattgaac ctaaatcaaa agctgaccaa gataaaatgg gtgtggcttt acaaaaacta
    28381 tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc
    28441 gctggtatgg gtgaacttca cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa
    28501 gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg aaactttccg tgctggcact
    28561 caagccgaag gtaagtttgt acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg
    28621 attgaattta caccaaatga agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt
    28681 ggggttgttc ctcgtgaata cattccagct gttgaaactg gtttgaaaga tgctatggaa
    28741 aatggtgtct tagctggcta tccattagta gatatcaaag ctaaacttta cgatggttca
    28801 taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt
    28861 gcagcagcta agaaagctaa tccagtaatc cttgaaccaa tcatggctgt agaagttgtt
    28921 atccctgaag attatttagg tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt
    28981 gaaggtatgg aagctcgcgc tggcggccaa caagttgttc gtgcgatggt tccacttgct
    29041 gaaatgtttg gttatgctac aactcttcgt tctgctactc aaggtcgtgg tacatttaca
    29101 atgacatttg accactacga agatgtacca aaatcagtac aagaagaaat cattaagaaa
    29161 aacggcggaa aagctgaata atttcttgaa accggatagt cctgcaaaga gattatccaa
    29221 agagactata tttttaatat agtttcgtgt aaaataatta tcgatgatat gggcatgtgg
    29281 tgtaccttgt gtacatcata tacctatcag acaaataaaa aatatattgt atttttagga
    29341 ggacatttta aaatggcaaa agaaaaattt gaccgttcta aaccacacgt taacattggt
    29401 actatcggac acgttgacca tggtaaaact acattaacag ctgcaatcac aactgtacta
    29461 tctaagaaaa acggcggcca agctatggct tacgatcaaa tcgatggtgc tccagaagaa
    29521 cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt atgaaacaga tacacgccac
    29581 tacgctcacg ttgactgccc aggacacgcg gattacgtta aaaacatgat cactggtgct
    29641 gctcaaatgg acggagctat cttggtagtt tctgctgctg acggcccaat gcctcaaact
    29701 cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac
    29761 aaagtagaca tggttgatga cgaagaatta ctagaattag ttgaaatgga agttcgtgac
    29821 ctattaacag aatacgaatt ccctggtgac gatgttcctg tagttgctgg atcagctttg
    29881 aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt
    29941 gacgaataca tcccaactcc agaacgtgac aacgacaaac cattcatgat gccagttgaa
    30001 gacgtgttct caattactgg acgtggtact gttgctacag gtcgtgttga acgtggacaa
    30061 gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca
    30121 gttactggtg ttgaaatgtt ccgtaaattg ttagactacg ctgaagctgg agacaacatt
    30181 ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac gtggacaagt tttagctaaa
    30241 ccaggtacaa tcacacctca tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa
    30301 gaaggtggac gtcatactcc attcttcact aactaccgtc cacaattcta cttccgtaca
    30361 actgacgtaa caggtgttgt tgaattacca gaaggaactg aaatggttat gcctggcgac
    30421 aacgtaacaa tggaagttga attaatccac ccaatcgcta tcgaaaacgg tactaaattc
    30481 tcaatccgtg aaggcggacg tacagttggt gccggtgtcg ttacagaaat caaagcttaa
    30541 ttcacttaag ttttaaagta aagcaacatt tattcggacg taatcgctcg gaacgaaggt
    30601 cttaggatct ttgttccgag cttttttatt tattgcagga tcaggaaaaa agacaggagt
    30661 attataaata gaagaaacac gaaataaaag agtcagtatt ttctaaatga aactttatga
    30721 aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga gtttatatat attataatga
    30781 caaaggtgcg tttactttag tgaattgcac agacgtaatc agaatgattt cgtcggctaa
    30841 gaaacgagag gttgcgacac gcccggacgc tttgccacga acgagcgtga cggaaatttt
    30901 cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg aacaagatgg caaaacaaaa
    30961 aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta gatcaatcag cggataaaat
    31021 cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt ccaattccat taccaactga
    31081 acgcagtctt tacacaatca tccgtgcgac tcataaatac aaagattcac gcgaacaatt
    31141 cgaaatgcgt acacacaaac gtcttatcga cattgtgaac ccaacaccaa aaactgttga
    31201 tgctttgatg aagctagact taccatctgg tgtaaacatc gaaatcaaat tataaaacta
    31261 aaacaaaaat aatggaggtg tactcatgac caaaggaatc ttagggaaaa aagtgggaat
    31321 gacacaaatc ttcactgaat ctggtgaatt aattccagta actgttgtag aagctactcc
    31381 aaacgtagta ctacaagtga aaacaatgga aacggatggc tacgaagcta ttcaagttgg
    31441 ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg aaaggtcatg ttgcaaaagc
    31501 aaacacggct cctaagcgct tcattcgtga attcaaaaat gttgagctag gagaatatga
    31561 agtaggaaaa gaaattacag tagatgtttt ccaagcagga gacattattg atgtaacagg
    31621 tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc cacggacaaa gccgcggacc
    31681 aatggctcac ggttctcgtt accatcgtcg tcctgggtca atgggtccag ttgcacctaa
    31741 ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt ggcaaccgcg taactatcca
    31801 aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt atcttgatca aaggtaatgt
    31861 tcctggagct aaaaaatctt taattactat caaatcagct gtgaaagcta aataattgta
    31921 ggaagaaagg aggaactaag gaatgccgaa tgtagcatta ttcaaacaag atggaagcca
    31981 aaacggtgaa atcacactta acgaagaaat cttcggaatt gaacctaatg aaagtgttgt
    32041 ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa ggaacacacg cggttaaaaa
    32101 ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt caaaaaggaa caggtcgtgc
    32161 acgtcaagga tctatccgct caccacaatg gcgtggaggt ggcgtagtct ttggaccaac
    32221 accacgttct tacagctaca aacttcctaa aaaagttcgt cgtttagcaa tgaaatctgt
    32281 attgtcagaa aaagttgctg aaaacaactt ggtagcaatc gaaggcttga acttcgacgc
    32341 accaaaaaca aaagaattca aacaagttct tgctaactta tctatcgact ctaaagtatt
    32401 ggttgttcta gaaaaaggta acgactttgc tgctttatca gcacgtaact taccaaacgt
    32461 ttctgtagta acttctgata atgtgagtgt gctagacgtt gtatcaaata caaaagtgtt
    32521 agcaacacaa actgctctta ctcaaattga ggaggtgctt gcataatgaa cttactagac
    32581 gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg ccatggatga caagaaatac
    32641 acttttgaag tggacactcg cgcaaacaaa actttagtaa aacaagcggt agaagctgct
    32701 tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac gtgcgaaatt caaacgtatg
    32761 ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga tcgttacatt gactgaagat
    32821 tcaaaagaaa tccaattatt cgaagctgct gaataagcac taatagataa ctcattaaat
    32881 taggagggaa aaacgtggcg attaaaaagt acaaacctac ctcaaatggt cgtcgtaaca
    32941 tgacaggttc tgattttgct gaaatcacaa catcaacacc tgaaaaatca ttgttacaac
    33001 cattgaaaaa ccatgctgga cgtaacaaca acggccgcat cactgtacgt catcaaggtg
    33061 gcggacacaa acgccaatat cgcgtgatcg acttcaaacg taacaaagat aacgttgttg
    33121 caactgttca aacgatcgaa tatgatccaa accgttcagc taacatcgcg ttagtacact
    33181 atgaagatgg agtgaaagct tacatcttag cacctaaagg actagaagtg ggtatgaaac
    33241 ttgtttcagg tccagaagca gatatcaaag tcggaaatgc tcttccatta gaaaacattc
    33301 cagttggtac tgttatccac aacattgaaa tgaaacctgg taaaggtgga caattgatcc
    33361 gttctgctgg tacaagtgct caagtacttg gtaaagaagg caaatacgta ttgatccgct
    33421 tgaactctgg tgaagttcgt atgatcttag ctgcatgccg tgctacaatc ggttctgtag
    33481 gtaacgaaca acacgaatta atcaacatcg gtaaagctgg acgctctcgt tggatgcgta
    33541 aacgcccaac tgtacgtggt agcgtaatga accctaatga tcacccacac ggtggtggtg
    33601 aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc ttggggtcaa ccagctcttg
    33661 gttacaaaac tcgtaacaaa aaagctaaat cagacaaact tattgttcgt cgtcgcaaaa
    33721 ataaataatt actttgacca tgtgtcgcac attttgagag gaggttcacc atgggtcgta
    33781 gtttaaagaa aggacctttt gtcgatgagc atttaatgaa aaaagttgaa gctcaagcag
    33841 gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg ttcaacagtt ttcccaagtt
    33901 ttgttggata taccatcgca gtttatgatg gacgtaaaca tgtaccagtt tacatccaag
    33961 aagacatggt aggacacaaa ttaggtgaat ttgcacctac aagaacttat cgtggacatg
    34021 ctgctgacga taagaaaacg aaacgctaat ttgaggggag gataaatcca aatggcagaa
    34081 caaatcacat cagctaaagc aactgcaaaa acagttcgca cttcacctcg aaaagcccgt
    34141 ttagtaattg accttatcag aggtaaaagt gtcgcagatg caatttcaat cttgaaattc
    34201 acaccaaaca aatctgctgg aatcattgaa aaagtattga tgtcagcagt tgcgaatgca
    34261 gaaaataact ttgacttaga tgttgaaaac ttggtagtaa gcgaagcttt tgttaacgaa
    34321 ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat cagcttctcc aatcaacaaa
    34381 cgtacaagtc atatcacggt agtcgtatca gaaaaataag gagggataat cagtgggtca
    34441 aaaagtacat ccaattggaa tgcgtgtagg catcatccgc gactgggatg ctaaatggta
    34501 tgctgaaaaa gagtatgctg agttcttaca cgaagattta agaatccgta aatttatttc
    34561 aactaaactt gctgatgctg ctgtatcaac agttgaaatc gaacgcgcaa caggtcgcgt
    34621 aaacatttca attcacacag ctaaaccagg tatggttatt ggtaaaggcg gttctgaagt
    34681 cgaaagccta agaaaagaat taaacaaatt aactggtaaa agaatccata tcaatatcgt
    34741 ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt gaaggaatcg cacgtcaatt
    34801 agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc atccaacgct caatgcgtgc
    34861 tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta aacggagcag atatcgctcg
    34921 ttcagaagga tactcagaag gaactgttcc tcttcatact ttacgtgcgg acattgatta
    34981 cgcatgggaa gaagcagata caacatacgg aaaactagga gttaaagtgt ggatctatcg
    35041 tggagaaatt cttccaacta aaaaaaacac tgagaaagga gggaaataat catgttagta
    35101 cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa tgcgcgggga agctaaaggc
    35161 ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg ttgattcaca ttggatcaca
    35221 aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt acatgaaacg tggtgggaaa
    35281 gtatggatta aaattttccc tcacaaatct tatactgcca aagcaattgg ggtacgtatg
    35341 ggttctggta aaggggcacc tgaaggatgg gttgcaccag taaaacgtgg taaaatcatg
    35401 tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag cgttacgtct agcttctcac
    35461 aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa tgggtggtga atcgaatgaa
    35521 ggttaaagaa atcagagaat taaccactgc cgaaatgctt gatcaagaaa aacaattaaa
    35581 agaagaattg tttaatctta gattccaatt agcaacaggt caattagaaa acactgcacg
    35641 tattaaagaa gtacgtaaat cgattgcacg catcaaaact gtgttgcgtg aacaagctaa
    35701 gtaattatgg aaggaggcca ttaagcgtat gactgaagga agaaatcaac gcaaagttta
    35761 tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt accgttgctg tcgaaacaaa
    35821 gaaaaaccac cctatctatg gcaaacgcat gaactattct aagaaataca aagtgcatga
    35881 tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt atggaaactc gtccattatc
    35941 agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa gcagttatta tctaataaca
    36001 cgagattttc ctatatagat tgaatgcaaa atctgaaagg aggatacaca acgtgatcca
    36061 agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca cgtgaaattt tgacgatcaa
    36121 agtccttggt ggatctggtc gtaaaactgc taacatcggt gatgtgatcg ttgctacggt
    36181 taaacaagca acgccaggtg gggttgtcaa aaaaggtgac gtcgttaaag ccgttatcgt
    36241 tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat attaaatttg atgaaaatgc
    36301 tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca cgtatctttg ggccagttgc
    36361 acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca ctagcaccag aagttttata
    36421 atcttgagac acttatcaaa ggaggtgcga aacagttatg tttgttaaaa aaggcgataa
    36481 agttaaagtt atcactggaa aagacaaaaa caaagaaggc gttgtactag cagcgttccc
    36541 gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt aaaaaacacc aaaaaccttc
    36601 tcaagctgct cctcaaggtg gaattgttga gatggaagcg ccaattcatg tttctaacgt
    36661 gatggtggtt gactctactg gtgtagctgg ccgagttggc tacaaagaag tagacggcaa
    36721 aaaagtccgt gtttctaaaa aaaccggtga agttttagat aaataaaagt ttaaggaagg
    36781 agggtcttac tgaatgaacc gcctaaaaga aaaatatctt aaagaagtaa ctccatcatt
    36841 gatggaaaaa tttaactata gctctgttat gcaaacacct aaagttgaaa aaatcgtcat
    36901 caacatgggt gttggtgatg cggtatcaaa cgctaaaaac ttagataaag cagttgaaga
    36961 attaactttg atcactggtc aaaaaccaat gattacaaaa gctaaaaaat caatcgctgg
    37021 attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt acattgcgcg gagaaagaat
    37081 gtacgaattc ttagacaaat tggtttctgt ttcactacca cgtgtacgtg acttccatgg
    37141 tgtaagtaaa aaagcctttg acggtcgtgg aaactatact ttaggtatta aagaacaatt
    37201 gatcttccca gaagtagatt atgatttagt agataaagta cgtggtatgg acatcgttat
    37261 tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg ttgacacaat taggcatgcc
    37321 attccaaaaa taattaaagg aggcgaacta cgtggctaaa aaatcaatga ttgctaaaaa
    37381 caaacgtcct gcaaaatttt caacacaagc ttatactcgt tgcgaacgtt gcggacgtcc
    37441 acattcagtt tatcgtaagt tccatctttg ccgtatttgc ttccgcgaac ttgcctataa
    37501 aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa gtaaattcgc ataagaagga
    37561 ggtaaaagat caatggtcat gacagatcca attgcagatt ttctaactcg tatccgtaac
    37621 gcaaacatgg ttaaacatga gtctttagaa gtacctgcat caaaaattaa acgtgacatc
    37681 gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg aatacatcga agatgacaaa
    37741 caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg gcgaacgtgt tatcacaaac
    37801 ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca aagctgacga agtaccaaaa
    37861 gtattgaacg gtttaggtat cgcaatcatt tcgacttctg aaggtgttgt cactgataaa
    37921 gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt acgtatggta atcaagtaga
    37981 atctacaagg aggtgtcttt aagtgagccg tatcggaaat aaagtcgttg ttatccctga
    38041 aggagtaaca gttactcaag acggaaacaa cgttacagtt aaaggaccaa aaggtgaatt
    38101 gactcgtact ttttcagcag atatcaaaat gaatatcgaa ggaaacgaag taactttcac
    38161 tcgtccaaac gacagtaaag aaatgaaaac aattcatgga acaactcgtg cgaacttcaa
    38221 caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg ttagaactaa tcggggttgg
    38281 gtaccgtgca caattacaag gatcaaaact tgtattgaac gttggttact cacatccagt
    38341 agaaatcaca ccaccagctg gagtaactgt tgaagtacca gctaacacac aagtaattgt
    38401 taaaggtgcg aacaaagaag aagttggcga attagctgca aacattcgtg gaactcgtcc
    38461 tccagaacct tataaaggta aaggtattcg ttatgttggc gaatatgtac gccgtaaaga
    38521 aggtaaaact ggtaaataat cgtttagaaa aattgcatag aagccagcag caggaaaaag
    38581 gcatttggct gaagtttacc ttcaaaaaaa tacattttta cttgtgccgc agcttctatt
    38641 tttcgaaacg tatttactgc ttgaaatagt agtacaataa aaataaagag gtgacaattg
    38701 tgattacaaa accagataag aacaaaacac gtcaaaagag acatcgtcgt gtacgtaaca
    38761 atatctctgg tactgctgag cgcccacgct tgaacgtttt tcgttctaac aaaaacatct
    38821 acgctcaaat tattgatgac gtagcgggtg taacgctagc aagtgcctct accttggata
    38881 aagaaatttc aggtggaaca aaaacagaag cagcacaagc tgtcggtaaa ctagttgctg
    38941 aacgtgcagc tgaaaaaggc attaaagtag tagtctttga ccgtggtgga tacctttacc
    39001 atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa tggactagaa ttttagaaga
    39061 aggaggaata ccattcatgg tttatatcga tccaaaacat ttggaattag aagaccgcgt
    39121 tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga cgtcgtctac gttttgctgc
    39181 tttagttgtt gtcggtgaca aaaacggaca cgtaggattt ggtacaggta aagctcaaga
    39241 agttcctgaa gcaatccgta aagcaattga agatgcgaag aaaaacttag ttgaagtacc
    39301 tatggttggt tcaacaatcc cacacgaagt aatcggtgct tttagtggtg gtagaatcct
    39361 aatgaaacct gctgtagaag gttctggggt tgctgctggt ggaccagttc gtgccgtatt
    39421 agaattagct ggggtagcag atatcacttc taaatcttta ggctcaaata caccaatcaa
    39481 cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa cgtgcagaag aagttgcaga
    39541 acttcgcggc aaatcagttg aagaattaat cggataagga ggacaacaat aatggctgaa
    39601 ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc aaaaccaacg cgatacagtt
    39661 aaagcgttag gtcttacaaa gattaacagt tcagtggtta agccttctaa tgaagcaatt
    39721 aaaggcatga tcaatactgt ttctcaccta gtggacgttg aagaaattta atttagaaaa
    39781 atcataacta gaacattaag gaggtgccaa acctatgaaa cttcatgaat taaaacctgc
    39841 tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt acttcatctg gtaacggaaa
    39901 aactgccgga cgcggacaaa aaggacaaaa agctcgttca ggcggcggtg tgcgtttagg
    39961 atttgaaggg ggacaaacac cattgttccg tcgtttacca aaacgaggat tcacaaacgt
    40021 gaaccgtaaa gactatgcag tcattaatct tgatgtattg aaccgctttg aagacggagc
    40081 tgaagtaact cctgttgctt tagttgaagc tggaatcgtg aaaaacgaaa aagctggaat
    40141 caaagtatta gctaatgggg aactaaacaa gaaattaact gtgaaagcag caaaattctc
    40201 taaagcagct caagaagcaa ttgaagcggc tggtggatca atcgaggtga tctaatgttt
    40261 agactattaa aagatgcttt taaagtaaaa gacattagat cgaaaatcct atttactgta
    40321 tttgtgttgt ttgtatttcg cctaggtgca catattactg taccaggcgt aaatgctgct
    40381 tcattgcaaa acctttcttc tttgccattt ttcaacatgt tgaacttggt aagtggtagt
    40441 gcgatgcaaa acttttccat tttctcaatg ggggtttcac catacattac tgcttctatc
    40501 gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg ttgaatggtc gaaacaaggg
    40561 gaagttggtc gtaaaaaact gaatcaagcg actcgatatc tgacattagt attggcattt
    40621 atccaatctg tcggtatcac tgctggattc aatcaatacg ctcaattagg tttcgtgaat
    40681 aatcctaacg tagcaactta cgtaacgatc ggtttgattc taacagccgg tacgatgttt
    40741 gttacttggc taggagaaca gatcacagaa aaaggtatcg gaaatggcgt atcaatgatc
    40801 atttttgcag gtatcatttc tagattgcct gaaagtatca gagaaattta tgaagattac
    40861 tttataaata ttgatccttc agacatatgg aaatctgcta tttttgtggc cattttacta
    40921 gtagcggtct tgttgatcgt gacattcgta acatttttcc aacaagctga acgcaagatc
    40981 ccgatccaat atacgaaacg cgtagctggc gcgcctacaa gcagctatct gccgttgaaa
    41041 gtaaatgcag ctggagttat tccagttatc tttgctagtt cgtttattgc tacgccaaat
    41101 gctgttttac aagcattgag cggtagattc ggttctgaaa actggtacaa agtgatgatg
    41161 gagatcttta actataatac agcacctggt gctacgattt atacgattct gatcgttgcc
    41221 ttcactttct tctacgcctt tgttcaagta aaccctgaga aattagcgga aaacttgcaa
    41281 aagcaaggaa gttatatacc aagcgtacgc cctggtaagg ggactgaaga gtatgtctca
    41341 agattactga tgcgtttaag tacagttggt gcagtcttcc ttggtttagt ggcattgctt
    41401 ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa tcggattagg cggtacaagc
    41461 ttactgatcg ttatcggtgt agctttagaa actgcgaaac agttagaagg cttaatgctg
    41521 aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt tgaggtttcc cctcaacaca
    41581 cgttctcaca catttattag gagggaaaac aatgaacctc attttaatgg ggctgccagg
    41641 cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct tatggtattc cgcacatctc
    41701 aacaggagat atgttccgcg cagcgatgca aaacgaaaca gctcttggcc tggaagcaaa
    41761 gtcttatatg gataaaggtg cattagttcc tgatgaagta acaaatggaa tcgtgaaaga
    41821 gcgactagct gaaccagata cagagaaagg cttcttatta gatggctttc cacgtacgct
    41881 agatcaagca aaagctctgg acgcaatgct aaaagatttg aataaaaaaa ttgatgctgt
    41941 catcgatatc catgttggtg aagaaatctt ggtcgaacgt ctagccggcc gatttatctg
    42001 cagcaattgt ggagcaactt accataaaat tttcaaccct acaaaagttg aagacacatg
    42061 tgatcgttgc ggtgggcatg aattctatca aagagaagat gataaacctg agacggttaa
    42121 aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc ttggcttatt ataaagaaca
    42181 aggtttgcta aacactattg atggtgctcg tgaaatcgat gctgtatttg cagatgtaca
    42241 aaaaatcatt gaaaaatagc acgattgccc gtagccgact tgcattatgc agttactctt
    42301 gtcaagttca ataaattatg atagaatatg tcagtccgac ttctagagaa tcctctagat
    42361 gatccatttt gagaattttg aggtgggaac tgcagtttcc gccattttat cgtgtgaaaa
    42421 tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga agatatgatt gaagtcgaag
    42481 gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt tgaactagaa aacggacacc
    42541 aagttcttgc tactgtttca ggtaaaattc gtatgcatta catccgtatc ttacctggtg
    42601 acaaagtaac ggttgaattg tctccatacg atcttaaccg tggccgtatt acttatcgct
    42661 ttaaataatt gtactccgta aaactacagg aggtattatc atgaaagtaa gaccatcagt
    42721 aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa ggacgcgtta tggtgatttg
    42781 cccagcaaat ccaaaacata aacaacgtca aggataattg gaggtgctat aaatatggct
    42841 cgtattgcag gagtagatat tcctcgtgat aaacgtgtag tagtttcact tacttatatc
    42901 tatggtattg gtaacactac tgctaaaaag atcttagctg atgctggtgt atctgaagat
    42961 gttcgcgttc gtgaattaac gaatgaacaa acagatgcta tccgtgcaga agtagataaa
    43021 ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga acatcaaacg tttgatggaa
    43081 atcggttctt accgtggaat ccgtcatcgc cgtggattgc ctgttcgtgg acaaaacacg
    43141 aaaaacaatg cacgtactcg taaaggccca gctcgcgcta tcgctggcaa gaaaaaataa
    43201 ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa aagtgaatcg taaacgccgt
    43261 gtgaagaaga atatagaagc aggtattgca catatccatt ctacattcaa taacactatc
    43321 atcatgatca ctgatactca cggaaatgca ttagcgtggt catcagctgg ttcattaggt
    43381 ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa tggcagcaga aactgctaca
    43441 aaagcagcaa tggaacacgg attaaagact gttgaagtaa cagttaaagg acctggttct
    43501 ggacgtgaag cagcaattcg ttcattacaa gctacaggtt tagaagtgac tgcaattcgt
    43561 gacgtgactc cagttcctca taatggtgcc cgccctccaa aacgccgtcg tgtttaatga
    43621 gtgccgctca ttttgagttt tatcagtaac gtcattgaac aagacacttt tcgttttgaa
    43681 aggggtaaag ataagaatga ttgagttcga aaaaccaaga atcgcaaaaa ttgatgaaga
    43741 aaaagattat ggcaagttca tcgttgaacc tcttgaaaga gggtatggga ctactttagg
    43801 gaattcccta cgtcgtattt tattatcttc tttgccaggt gctgccatca ctaatattca
    43861 aatcgatggt gtactgcatg aattctctac catcaaaggt gttagagaag acgtcactca
    43921 aatcatcttg aacattaaag gcttagcgct gaaaatgtat ggccaagaag aaaaaaccct
    43981 tgaaatcgat attaccggac ctgctacagt aactgctggc gatattatcg ttgatagtga
    44041 tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc tctgaaggag ctacgttcca
    44101 tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa gcagatgaaa ataaaaaaga
    44161 agatatgcca attggcgttc ttccagtcga ttcaatctac acacctgttc gtcgtgtgaa
    44221 ctatcaagtt gagaacacac gtgttggaca ccgtgaagat tttgataaat tgacgatgga
    44281 gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg agtttgtctg cgaaaatcat
    44341 gactgaacat ttggatattt ttgtgaactt aactgatgaa gcaaaaaatg ctgaaatcat
    44401 ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa atgacgatcg aagagttaga
    44461 cttgtctgtt cgctcttaca attgtttgaa acgtgcagga atcaatactg tacaagaact
    44521 tacaaataaa tctgagcctg aaatgatcaa agtccgtaac ttaggacgta aatcattaga
    44581 agaagttaaa gcaaaattgc acgacttagg tttaggttta cgtaaagaag attaaaacct
    44641 tttacgaagg agggaaacca acgtgagtta tcgtaaacta ggacgcacat ctagccaacg
    44701 taaagcgatg ttgcgtgatt taacaactga tttattaatc aacgaacgta ttgtgactac
    44761 tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa atgatcactt taggtaaacg
    44821 cggagatttg catgcacgtc gtcaagctgc ggctttcgta cgtaatgaag ttgccagcgt
    44881 gcgtgaagaa aatgaggaca tcgtcattga atcagcttta caaaaactgt tcaacgatat
    44941 tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc attttgaaga cagaaccaag
    45001 acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc taaaatcaca ctaataacat
    45061 cactttatag atgattcttt tagagtgtta tgatgttgga agatattttt ccgagtctag
    45121 ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa gcactcatca taaagtgttt
    45181 ttttatacct tcaattacct gtcatgacag atatgtttga aggcataaaa aaacatttta
    45241 agcgagaaat aattgaaaat tttgtctttt tttgatagtg ttagtaggaa ctttaggatg
    45301 aagaaggagt aatcatggag ccaattattg aattagaaaa gataaattat aaataccagc
    45361 cagatgatct tcgccccgca ttgaaagatg tctcctttac aattgataaa ggtgagtgga
    45421 tcgctattat tggacacaat ggttcaggaa aatcgacact tgccaaaacg atcaatggat
    45481 tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca aatattggat gaagaaaata
    45541 tttggacgat ccgacagatg gttggaatgg tttttcaaaa tccagacaat cagtttgttg
    45601 gttcgacagt agaagatgac gttgcttttg gtcttgaaaa tcaagggatt cccagagaag
    45661 aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag aatggctgag tttgcttcac
    45721 gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg
    45781 ccttacgacc tgatatcatt attttagatg aagcaaccag tatgctagat ccagaaggtc
    45841 gtgaagaagt gatctctacg atcaagaaga tcaaagaaga aagtcaattg accgttatat
    45901 ctatcactca tgatattgat gaagcagcta atgccaatcg gatactagtg atgagacaag
    45961 gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc tgctggtcct gaactgatcg
    46021 acttaggtct agatctacca tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg
    46081 ttgatgtgcc cagtgaatat atgacagaag aaaggatggt ggattggtta tggacatccg
    46141 ttttgaacaa gtagacttca cttatcagcc gaatacacca tttgaacaaa gagccttatt
    46201 tgacatcaat atgacgatca aagaaaacag ttatactgca ttagtagggc ataccggaag
    46261 cgggaaatca accttacttc agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt
    46321 ccatattgga gaacgagata ttcagccgga tacggataat aagaatttaa agcccatccg
    46381 aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag ctattcgaag aaacggtagc
    46441 aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc gaagaagaag cactagtcct
    46501 agcaaaagaa acattagaac aagttgggct ggatgaaagc tatttggaac gttcgccatt
    46561 tgaactttca ggagggcaaa tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc
    46621 ggaagtactt gtattggatg aaccaacggc aggacttgat ccccagggca gaaaagaaat
    46681 gatggagatg ttttggcgtc ttcataagga acagcagatc actattgtat tagttacgca
    46741 tttaatggat gatgtagcca attttgcaga ttatgtctat gtgttggaaa agggacgaat
    46801 cgttaatagc ggggagccgc aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca
    46861 actaggcgta cctactgcca ccgagtttgc agaagaatta atggctaaag ggatgaactt
    46921 tgctaccttg cctttgacag ctgaagaatt agcagacgct atcgtccatt atgctggagg
    46981 aaatgcccat gatgaataaa ttgatatttg gtcgttatat gcctggagat tccttgatcc
    47041 atcgattgga tccccgagct aaattattag ccagttttta ttttatcgga atcatttttt
    47101 tagcaaataa ttgggaaacg taccttttgt tggcattttt cacattactt tctgtcttct
    47161 tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg cccattgatc tggctaatcc
    47221 tcttcactgt agctttgcag gtcttgttta caagtggcgg agaaacttat tggagttggg
    47281 gtgttttcca tatcactgaa tttggtgtga tgaacggcct ctttattttc tgtcgattcg
    47341 tgttgatcat ttttatgtcg actctactca cgcttactac accgcctttg gaattatcgg
    47401 atgcgattga gtacttgctt cgtccattga aagccgtacg gttcccagtc cacgaaattt
    47461 cattgatgct gtcgattgct cttcgttttg tgcctacatt gatggacgaa acagaaaaga
    47521 tcatgaacgc tcagcgcgct cgcggtgttg attttggtga aggaaaccta gttcagaaaa
    47581 tgaaagcagt cattcctttg ctgattcctt tatttgtcag cagcttcaac cgtgcggaag
    47641 atcttgcgac tgctatggaa gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc
    47701 gtgtattgca ttggcataat agagacacga tggttatcat tgcttttgta ttattaactg
    47761 tgctactgat ctttttaaga ggataaacgt tcagtgggcc gctatctatt cttttcttgc
    47821 tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa gaaatattag ggtctgtggc
    47881 agactaaggt cacaggcctc tttcttttat ctgaaaagaa agaacgaaaa aattttatag
    47941 aaaaatcatt attccagata accttttagg aacaaaggag agaaaaacat ggtacgttac
    48001 aaagcaatca ttgcgtacga cggaacaaat tttaatggat tccaaaaaca gccaaatggg
    48061 cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa tggcaaatgg aaaagaaatc
    48121 acggtctttg gttcgggaag gacagatgcc ggagtacatg cgatcgggca agtaatccat
    48181 tttgattatc cagaggagcg tccgttagaa cgaatgcgtt tcgcgctaga tacccagtca
    48241 ccagaggata ttgctgtaag acaagtagaa attgtttcag aagacttcca cgcaagatat
    48301 ttggtcaaag aaaagacgta tcaatttcga gtggatatag ggaaaccacg cagtcctttt
    48361 cgacgccatt atgcaagtta ttttccttac ccacttgatt tggaaaaaat tcagcgggcg
    48421 cttcctgatt tattagggac tcacgatttc acctcttttt gtgcatcagg aagctcgata
    48481 gaggataaaa tacgtacgat ctacgaggct aagatggagg taaatgaaac aaaagacgaa
    48541 ttgttgttta catttagggg aaatggcttc ttgtataaga tgatacggat cttagtagga
    48601 acgcttttga aaattggcaa tggccgattg cctgaagata gtatcccaga tattattgcg
    48661 aaaaaagatc gtaatgcagc aggacctaca gcacaccctg aaggacttta tttgtatgaa
    48721 gtgaaatatg aataagaggt tatgatagaa gtggtcagct tcaagaaata agaaggtatt
    48781 tacgaaaatt acttctcaaa attttgcaaa atttcggctt attttcgaag aagctacttc
    48841 tggaacaccg tttaaggaag aggtcgtgaa atcagcgttt tgacctgagt attagagaaa
    48901 caaacgggaa aatagctcct catattttgc cgtttgttga gctaataccg aaggtcagct
    48961 gattgaacac cgtttaagaa agaggtcgtg agcctgtcat ttaactctga gtcacaaaga
    49021 gcaatttcaa aaaacagctt ctcatctttg atgaatatcg tgacttgtgg acgaggcgtt
    49081 gataagtgaa tcatcttttt gttacctatt ttttctgtaa ccaaaaaacg atggaacgtt
    49141 gttttgattg cccttggtgc ggacagtctg aacaggttcc atcgtttctt tattttctac
    49201 gtcttcttct gtgtgtgttt cgtggttgtt cttctattgc tggatcaatc gtgccataaa
    49261 aggggcgatt gcggtcaagg taccagacat aaagaataaa caatgcaata cataggacaa
    49321 aaaggattgc acctatcgta aatccttcag gcaagtaact gaaagtgaca gtatgtttac
    49381 ctgctggaac ttttatcatc aagaatgcgt ctttgaaatt gctgaccttg actggtttcc
    49441 catcaatttt agctttccat cctttgtcat aaggaatagt tgtcaccaac atggaatctt
    49501 cagcggtatt tacagtccca gaagcttttc tgccagtggc ttccatttcg acaccttttt
    49561 ctttgatctg attcattgcg gcttgatagg ctacaacatc tagaccaacg actttaggct
    49621 ccatgaaact tatttctttc gtcccataaa aacttgcagt aaatgtcact gttgtctctt
    49681 gaggataata gccaagatca tagtattgac cagaaatgtt gatttgcgtt tttcgcgatg
    49741 taccattgac agtgatcgtc gcactagaac tttctaattg cgcaaaattg gttgggaaca
    49801 aacttaagta agcttgtgta tttgcaggca catggacttc ccaagtgagc tcttttggct
    49861 ggttatctgc tgtttctgag taagtgacgc cagtcgttgt ctttgtgatc gtcgtattca
    49921 actgattctt caaagtaggt gtatagaatg taaaatactg ttcatttaga ccagacaatg
    49981 tattgatcaa gtttgtctga ctggtcaaat tatctgtcac tggctgctca acatcattaa
    50041 cggacaacgg tgcagtaaat gctagtggca atgcattgac attctgatac agtgtatagt
    50101 cgccgctttg accagcttgg cggaagccat atttactgaa cgtgctttcg tttttagaaa
    50161 tgttatattt gattcctgta aacccatcca tcagcaaagt attattgtta taacggatat
    50221 tcaaatttgt ccctcttgaa cggaaaccta atttatccag gtaagaggaa gaattacgat
    50281 ttcttataga agaaaacaaa ctgataccac tgtaaccata gttgatcgaa tcgtttgcag
    50341 atactggatc cagattttcc aagcggtaaa acgagtcgga atcttttttt gtttcattta
    50401 caagagcttt caagtcagga taaggatcag tatataaact gcgggaagca taattccaat
    50461 catcaagaat gccatggatc atactgttcg tattgaccag tgcttcagca gacatgatca
    50521 ataataacaa tatactcaga tgattgaatg aaagtttttt atgctgataa aatcccacag
    50581 caaaagcata aatccctaag aaaaccactg tcaatacaaa ggaagtcatc gtcacatagg
    50641 tgtagcttcc ttcaggtttg atgccccatg ccaccgcaaa gagagcagct acgaccaaaa
    50701 tgattcctaa caacagccca cgatcgtctt gttctatctt ttcccatcct tttcctgcaa
    50761 gcaggagcac taagaaagag aacagatagg cataacggaa taaaaacata ttcggtgcat
    50821 gcataccatg ccagaacaaa ttcaatggaa caagataaaa acttgcgata agtatggcga
    50881 aaagactgcc gtacaaaaat ttatttttca acggtacttt ccgactggca aaataaaaca
    50941 gacagaggac taatggtagc agacctgcgt aaacgaacgg aatcgatccg tattttgtcg
    51001 tgtcataaac accaatcata tttttcatga ttagatccag ataagaagtc gcttctgttt
    51061 tcaacgtagt gatctgactc agtgcctcgc catttgaccg aagatccagc actgctggca
    51121 atacaatgat cattgaagcg ccgcctgcca gtaaagaagt gataccataa ggaacaatcg
    51181 tttttttata ttctgaccag tttgtcaata gacgggcgat aaaatataag aaagaaaata
    51241 cgccaatcat aaaacccata taaaaactgg ataaaaacaa gagtaaataa ctgataaata
    51301 aaagtttcgg ccgtttaaga tccataacac gatgaatccc caaaatcact aatggaagat
    51361 aaaaaaatgc atccagccac atgatgatct ccgaatgtgc agtgataaat gagatcaatg
    51421 cgtaactgac gctaagtgca acatgccccc agcgaggaat cttgaacgta cgtgcaagaa
    51481 accaaaaagc caatccggca cagccgacct taagcaaagt cagaacatac agtgcatctg
    51541 gcatcatctg gtttggaaaa aagaacacca gtggcgtgaa gatcccgcct agatagtaag
    51601 aaattaaaga taaataattc aatcctagag aagcattcca tgtataaaaa atactttgct
    51661 ttccaagaag aacatttcgg aagctggcat gaaaattaga aaactgtgaa aaggcatcac
    51721 ttgccaaaat acttctgctg cttccaggat agattccgat acttagataa acaagtgcta
    51781 aaatcaaaaa cggaatcagg aaactagctg ctgcgtagcg tccgttttct ctaaagaatg
    51841 actttaaatt ttttttcata tttattttcc accaaatttc tatgaagttg tattcagttt
    51901 atcatatcct tgtttt
//
LOCUS       DAXLPI010000005        50875 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXLPI010000005 DAXLPI010000000
VERSION     DAXLPI010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.005"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            70..3348
                     /locus_tag="ACF3T5_000241"
     CDS             70..3348
                     /locus_tag="ACF3T5_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFD3630884.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3889..5796)
                     /locus_tag="ACF3T5_000242"
     CDS             complement(3889..5796)
                     /locus_tag="ACF3T5_000242"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFD3630885.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5926..6801
                     /locus_tag="ACF3T5_000243"
     CDS             5926..6801
                     /locus_tag="ACF3T5_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFD3630886.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6881..7855
                     /locus_tag="ACF3T5_000244"
     CDS             6881..7855
                     /locus_tag="ACF3T5_000244"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFD3630887.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8035..9180
                     /locus_tag="ACF3T5_000245"
     CDS             8035..9180
                     /locus_tag="ACF3T5_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFD3630888.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9317..9661
                     /locus_tag="ACF3T5_000246"
     CDS             9317..9661
                     /locus_tag="ACF3T5_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFD3630889.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9932..11218
                     /gene="efmA"
                     /locus_tag="ACF3T5_000247"
     CDS             9932..11218
                     /gene="efmA"
                     /locus_tag="ACF3T5_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFD3630890.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11356..11955)
                     /locus_tag="ACF3T5_000248"
     CDS             complement(11356..11955)
                     /locus_tag="ACF3T5_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFD3630891.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12359..13480
                     /gene="mnmA"
                     /locus_tag="ACF3T5_000249"
     CDS             12359..13480
                     /gene="mnmA"
                     /locus_tag="ACF3T5_000249"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFD3630892.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLIYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13726..15147
                     /gene="arcD"
                     /locus_tag="ACF3T5_000250"
     CDS             13726..15147
                     /gene="arcD"
                     /locus_tag="ACF3T5_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFD3630893.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15161..16279
                     /locus_tag="ACF3T5_000251"
     CDS             15161..16279
                     /locus_tag="ACF3T5_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFD3630894.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16339..17631)
                     /locus_tag="ACF3T5_000252"
     CDS             complement(16339..17631)
                     /locus_tag="ACF3T5_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFD3630895.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17781..18341
                     /locus_tag="ACF3T5_000253"
     CDS             17781..18341
                     /locus_tag="ACF3T5_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3630896.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18470..18847
                     /locus_tag="ACF3T5_000254"
     CDS             18470..18847
                     /locus_tag="ACF3T5_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFD3630897.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18780..20852
                     /locus_tag="ACF3T5_000255"
     CDS             18780..20852
                     /locus_tag="ACF3T5_000255"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFD3630898.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21063..22010)
                     /locus_tag="ACF3T5_000256"
     CDS             complement(21063..22010)
                     /locus_tag="ACF3T5_000256"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD3630899.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22256..22720
                     /locus_tag="ACF3T5_000257"
     CDS             22256..22720
                     /locus_tag="ACF3T5_000257"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFD3630900.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22782..23825)
                     /locus_tag="ACF3T5_000258"
     CDS             complement(22782..23825)
                     /locus_tag="ACF3T5_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFD3630901.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24202..25167)
                     /locus_tag="ACF3T5_000259"
     CDS             complement(24202..25167)
                     /locus_tag="ACF3T5_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFD3630902.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25401..26246
                     /locus_tag="ACF3T5_000260"
     CDS             25401..26246
                     /locus_tag="ACF3T5_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD3630903.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26437..27624
                     /locus_tag="ACF3T5_000261"
     CDS             26437..27624
                     /locus_tag="ACF3T5_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFD3630904.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27630..27986
                     /locus_tag="ACF3T5_000262"
     CDS             27630..27986
                     /locus_tag="ACF3T5_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFD3630905.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27988..28326
                     /locus_tag="ACF3T5_000263"
     CDS             27988..28326
                     /locus_tag="ACF3T5_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFD3630906.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28402..28658
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28721..29755
                     /locus_tag="ACF3T5_000264"
     CDS             28721..29755
                     /locus_tag="ACF3T5_000264"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFD3630907.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29748..30434
                     /locus_tag="ACF3T5_000265"
     CDS             29748..30434
                     /locus_tag="ACF3T5_000265"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFD3630908.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30458..31306
                     /locus_tag="ACF3T5_000266"
     CDS             30458..31306
                     /locus_tag="ACF3T5_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFD3630909.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31503..32276
                     /gene="sufC"
                     /locus_tag="ACF3T5_000267"
     CDS             31503..32276
                     /gene="sufC"
                     /locus_tag="ACF3T5_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFD3630910.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32289..33575
                     /gene="sufD"
                     /locus_tag="ACF3T5_000268"
     CDS             32289..33575
                     /gene="sufD"
                     /locus_tag="ACF3T5_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFD3630911.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33572..34807
                     /locus_tag="ACF3T5_000269"
     CDS             33572..34807
                     /locus_tag="ACF3T5_000269"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFD3630912.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34794..35264
                     /gene="sufU"
                     /locus_tag="ACF3T5_000270"
     CDS             34794..35264
                     /gene="sufU"
                     /locus_tag="ACF3T5_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFD3630913.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35269..36660
                     /gene="sufB"
                     /locus_tag="ACF3T5_000271"
     CDS             35269..36660
                     /gene="sufB"
                     /locus_tag="ACF3T5_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFD3630914.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKAVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36755..37897)
                     /locus_tag="ACF3T5_000272"
     CDS             complement(36755..37897)
                     /locus_tag="ACF3T5_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3630915.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37936..38670)
                     /locus_tag="ACF3T5_000273"
     CDS             complement(37936..38670)
                     /locus_tag="ACF3T5_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD3630916.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIIEEFDNNILLLTSSIEEQLEEMIDEGIHSQVEDMNYPEEAVNEIIDN
                     INKLKQTISSIGKKYTKKKMKGGDTI"
     gene            38905..39180
                     /locus_tag="ACF3T5_000274"
     CDS             38905..39180
                     /locus_tag="ACF3T5_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3630917.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            39278..41275
                     /locus_tag="ACF3T5_000275"
     CDS             39278..41275
                     /locus_tag="ACF3T5_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630918.1"
                     /translation="METPMINIPINVDKERLLEKPVIEDEKKRIRKRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNKSEEFLETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKALSINLVHNSAFAIQKSIRYSENLEKFKIVFLLKEAITDYSKMIAVYEYLKKI
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHDDHNPSASIFTSKTGHSIFYCHSSNCGISENFIGVVMLILEKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMEDKLFRTINNYLEEMRRVYDALLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKWNKLLSFMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLKKVHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNFRSELSKEKQLILDEAHEVLQDLSWQGTYLMTEKELINTVC
                     ERLRNVGNNSVENALKENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELIASGVELVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41351..41686)
                     /locus_tag="ACF3T5_000276"
     CDS             complement(41351..41686)
                     /locus_tag="ACF3T5_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD3630919.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            41812..42027
                     /locus_tag="ACF3T5_000277"
     CDS             41812..42027
                     /locus_tag="ACF3T5_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD3630920.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            42064..42426
                     /locus_tag="ACF3T5_000278"
     CDS             42064..42426
                     /locus_tag="ACF3T5_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFD3630921.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADEVKKSTEQTFS
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            42472..42798
                     /locus_tag="ACF3T5_000279"
     CDS             42472..42798
                     /locus_tag="ACF3T5_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630922.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            42999..43775
                     /locus_tag="ACF3T5_000280"
     CDS             42999..43775
                     /locus_tag="ACF3T5_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630923.1"
                     /translation="MMKKNYLKTGIFLVIGTILVIQLSGCRNKDKDFYGYWSGTAEKI
                     PVDYNFKITKDRVIELQKNGEMVDFKYTLRHKKFDSTTFRSIMISKDELVKAIPELKE
                     TTGISNITFMPIDGEKDKLTVLVNLDEHLSTATLYRTTKDESPIGKVEKKNDTISRQT
                     VDKKIDTFIKTYAAIPPTWKLVNEDKGITINTDTEIPTDAQSLSDLLANLTGVSNAVS
                     EQVGKDIPLRLLENGKKDYSVVFKNGKVTDYNEIFEKVIE"
     gene            45531..46316
                     /locus_tag="ACF3T5_000281"
     CDS             45531..46316
                     /locus_tag="ACF3T5_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HFD3630924.1"
                     /translation="MVEDMVMVEEEQGRLIKSKKRVQKHGEVFTPQRTVKDMLDLPGI
                     KEACEELQTTFLEPSAGEGAFLVEVLTRKMAMVAREYNESLEQYENYSLLALSTLYGV
                     ELLEDNAQTCVMNMFQVFNDLYREQSKKHGTAMKRKVQDSAKTLISRNIKQGDFLTKR
                     TPDDNPIIFSEWTPKTLRKGQKKITVNRTEYSLEDIYSGVTREFGEAYSALPVFEQLD
                     LFSFDDEVDEEVSNDPILRYVAMPITEVYKEEMEEIDDDSVEI"
     gene            46294..50691
                     /locus_tag="ACF3T5_000282"
     CDS             46294..50691
                     /locus_tag="ACF3T5_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019788126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFD3630925.1"
                     /translation="MTTALKSKAKYFDYLPKYGSKLEHFLGIPNLVDAINTYRVTVNQ
                     IPNEVAIGTRRYSEVAARKIASNVGIDQQSMKYVDVLRELRVRNNITPTIADLFHKLR
                     KYGNVAAHENGIIPVSEAVESLVTLDTLLRVFARNLSGDKSLNPKEEITDEMFVITYN
                     TFDRKLIYIQSAKDADGTWKMYQGLEKIGDASVPENYEADFTPNSEYLREYASRRIKQ
                     YMTTAGVPFVLHWAQLAVNKKNQFFRDHDVHEVLKRSGYNPQTIGEDDTGKPNEWFSV
                     TVDVAKKAIQAVKDGKGSLDSEKDDFDEFKINFRPEQKDAIEQTKTVFKTKDEMLWNA
                     KMRFGKTLSSLQVIKESQYKKVLIMTHRPVVSDGWFTDFKKIFTDGSYIYGSKQDGEK
                     IQNLVDTEKPFIYFASIQDLRGSEWAGGKAGDKNYEFLAIDWDFIIIDEAHEGNETEL
                     ANNVKAKLRKEHTKVLELSGTPFNLFDKYDEDNIFTWDYTMEQEAKEKWSVEHPNEPN
                     PYEGLPKVSMFTFEIPNKFDYFDENKAFNFREFFRVKEDDETKLLHEGDVRKFLDYIT
                     TNDEKTNFPFSKPEFRENLRHTLWLMPGVKEANAFERVLETHPIFKDYNIANVVKTGD
                     SEHASDSDLELVRKAIGNPAKSKTITLTVRKLTTGVNVPEWTGVFFLSNTESPTSYLQ
                     AAFRAQTPFNHAELGIKKNCYIFDFAPDRALKIMSGSVGLTSKKGKVNSSEQKKKLER
                     MLNFLPILGKQGNVMKEFSVDRMLTALKKAYAEKAVRTGFEDSSLYNDNLLNLDQADL
                     TKFEDLKKIVGSSKPTKVKDFIVSDNGMSEEEYEKASRAEKKPKRQRTKEEQEAIDRA
                     KRLREQRNKMISILRGVSIRIPMMIYGMDIDIKDDVTIEMFLNLVDEASWNEFMPEGL
                     SKGRFREFIEYYDADVFVEAGRIIRRRAKSYDELDVIERTRKIGELFSTFKNPDKETV
                     LTPWRVVNMHLGNTIGGLSFFDEEYQNSTENGQPIVRWRKTDCTDDIFKENRKILEIN
                     SKTGLYPLYAATSMYYVQLLKQSDINAGRVDSEAIWEKVLSEQIFVISKTPMAKTITK
                     RTLIGYNAWKVNVEYSSELVTSMKRSINDGLTNLKGVYKNVKFDVIIGNPPYQEKDGG
                     SGASATPLYNLFVETSVKLSERYVSMITPSKWFAGGKGLDKYRDFMLNSSSLKVMVDI
                     VNGKEIFPNTSIGGGVSYFLWDKKYNGLTQYTNQHDGVSNTQERKMNEFPVFVRYNEA
                     ISAIHKIGEHASVGDFVISRNPFGLPTKIRGEEVQDSRNSIKVISSGGVGFVPLEDIT
                     KGQDMVGKYKVISSSLTSEHAGEPNKNGMFKVISTNRVIKPNEVVTDSYLVLFSADTE
                     TEAINYLKYLRTRFYRFLLLQAVTSIHLSKDKFMFIPVQDFTSGSDINWNVGIEEVDM
                     QLFKKYSFDASEILFIDQTIVPILHEEYY"
BASE COUNT        16824 a         8878 c        10717 g        14456 t
ORIGIN      
        1 ttgacagtaa aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga
       61 ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg
      121 ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg
      181 aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga
      241 actagtgaag atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat
      301 gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga
      361 gaaagcacaa gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct
      421 tccacgaata acggcggaaa attccagtca agcggatcag atggaatgac ctattattat
      481 caagcaatcc ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg
      541 acctatacga atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat
      601 catttgtttg ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa
      661 tatgtctggg actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg
      721 cgacttggat tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg
      781 ccagctcctg ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg
      841 gaaaaagaaa caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc
      901 acgaactcga ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca
      961 tattattacg atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa
     1021 aaattatacg cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg
     1081 acgatcaagg tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg
     1141 caagaaagac ctactcgctt gattgatccg atttacactg tgacctctag taatcattca
     1201 ggaaaccaag atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa
     1261 aataaagcaa caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg
     1321 gaatttgatt atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg
     1381 ccagataaaa actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag
     1441 attcttcata cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa
     1501 gggtcagatg caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga
     1561 tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga
     1621 ttgacgattc caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag
     1681 aaaaatccag aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca
     1741 ctgttcagtc actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa
     1801 aaaggattac agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat
     1861 ggaaatacag gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg
     1921 cataacttag tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc
     1981 gatggatttg cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg
     2041 tatcataatg cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga
     2101 aaagtaatca tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga
     2161 gaaggtaacg gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg
     2221 atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat
     2281 attatcgtca atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc
     2341 tcttcagcag gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat
     2401 cttgagatga gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg
     2461 ttgaactact attggaatga acgagagaaa caatcagaaa atactcttgg acataccata
     2521 tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg
     2581 cgctatgaaa atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt
     2641 caaacagaaa aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga
     2701 gaaacctcac cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca
     2761 aaaccaaatg aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat
     2821 gcagaaggaa atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca
     2881 aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt
     2941 gaaacacagt caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa
     3001 ggagaatata cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct
     3061 gctcatacag aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg
     3121 cccccgggaa atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa
     3181 ccaaatggaa aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact
     3241 caaggactcc caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt
     3301 ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc
     3361 cttttatgat gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata
     3421 gatgtctcac ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc
     3481 tgttcttatt acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa
     3541 tcagctgacc ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt
     3601 tttttctcta atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca
     3661 aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca
     3721 aaatttttct tactacttga aactaagcag ctttttcaca acctctaata catggtattc
     3781 cagaagtaac ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc
     3841 ccaaattcct tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg
     3901 cactcttttt tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata
     3961 gaattcgtca aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc
     4021 gatttgcagt aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca
     4081 attgacaaag aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca
     4141 gcttagaccg cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc
     4201 gtcagcaaaa ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg
     4261 tgtaagatct tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc
     4321 gttattatca aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt
     4381 agcagagctt gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac
     4441 gatcacattc cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc
     4501 tgaacgatcc actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa
     4561 aacaagagta tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc
     4621 gaataatgct gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat
     4681 gacggctttt tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata
     4741 aacggcgatc atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata
     4801 tttttttccg tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc
     4861 tgcttttttc tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct
     4921 gacttggcgg aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat
     4981 ccagcttttg aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta
     5041 cactggatca gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt
     5101 tttcaagtcc agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat
     5161 tttgctgtaa tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa
     5221 ttgcactgct tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa
     5281 taactgaaca gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga
     5341 tgcataatac gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt
     5401 ttgcgcattc ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa
     5461 cagccattct ggaaattgct tattcaattc gtcgaaagac tgcaatgatt caactttttc
     5521 gattataggt tgaagcggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag
     5581 ttgataatat gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag
     5641 cagatcgtct gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc
     5701 gcctgtagct ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc
     5761 ttcatagaaa tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt
     5821 cgtgtgccca ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa
     5881 ttcgaacaat caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg
     5941 cgatcccaac tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt
     6001 aaaaaattag ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct
     6061 ctggtttttg aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat
     6121 tctccagcaa aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat
     6181 gaagagccaa tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc
     6241 gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt
     6301 tttttgaggt ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca
     6361 gcagttctcc ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt
     6421 cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa
     6481 ccactaggac ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag
     6541 aagaatggca atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca
     6601 aaacgtatac gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca
     6661 tatggcaatc attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat
     6721 tacaaagtac acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac
     6781 ggaacagttt cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg
     6841 ttaaactatc gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga
     6901 tgacacgttg aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa
     6961 agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc
     7021 catcaatatc gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc
     7081 acctgtcaat gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag
     7141 tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc
     7201 gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc
     7261 aactcgagta ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc
     7321 tgtagataat ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac
     7381 gggagatgac atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct
     7441 ttcagaatat ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga
     7501 gggtggttat gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt
     7561 gaacacagga gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga
     7621 agtatatgcc tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa
     7681 tgcaccaatc aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc
     7741 agaaaactta acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca
     7801 tgaaaataca ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa
     7861 aaaaagaggg aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt
     7921 ttgtcacagc tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc
     7981 tttggtttgt tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat
     8041 gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt
     8101 gaagcgatga cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt
     8161 ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa
     8221 gcaaaacctc acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc
     8281 ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa
     8341 catcctgctg ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat
     8401 ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa
     8461 gatacgatct tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc
     8521 aaagaaatcg gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa
     8581 gcgtacggaa accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct
     8641 gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat
     8701 atccctgcat tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat
     8761 ttagcaggga tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa
     8821 gcacgtaaca acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac
     8881 gaaatccctt atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt
     8941 cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct
     9001 atttctactg gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca
     9061 atgtatggag aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga
     9121 aataccgaag acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa
     9181 ctagtgaagt cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa
     9241 cttaactgaa aaacctagta aaaatcagga taaactttta taataaagag aaactaattt
     9301 tatcgaggtg aaaatcatgg catttgaaac aacagcaacg gtactgggcg caccaattag
     9361 ctatcgtttg catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac
     9421 aaacggaggc aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg
     9481 attcaaatta aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac
     9541 agcaaatgga ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga
     9601 taaattttat tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg
     9661 atctgtggat ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt
     9721 gtaaggacga cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt
     9781 ttaatatttt gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat
     9841 ttagggaaaa aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct
     9901 atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac
     9961 gaattggaag agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag
    10021 tatggtcgtc caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat
    10081 tttgagtttt gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg
    10141 accgctagtg gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc
    10201 gattttcgcg cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct
    10261 agtttttgtg tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca
    10321 gtcgattatg ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg
    10381 aatggtccaa tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt
    10441 cgttcctgtc aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt
    10501 attgattttt gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat
    10561 ttttaaagat gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat
    10621 aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt
    10681 gatgacgcta gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta
    10741 cgcagtcgga atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg
    10801 aatgaaacct attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt
    10861 ggttccaaac gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg
    10921 tgcaacgcca tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa
    10981 tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg
    11041 attattattt gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc
    11101 cggaatcggg attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta
    11161 tgataaagaa ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta
    11221 tggaatggag ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt
    11281 ggcaataagc ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga
    11341 tatagggcag ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat
    11401 ttgttgctca gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg
    11461 tgcgtctacg cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc
    11521 atgaagattg cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata
    11581 ttgttgatgg tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc
    11641 gtcagtaagc ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt
    11701 ccctttgatc aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt
    11761 tgctttttca tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc
    11821 ttttattact gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa
    11881 gagccgcgga gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact
    11941 gataaagtaa ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta
    12001 tctgagcaac tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc
    12061 gataagaagc aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg
    12121 aaaggctggc cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt
    12181 gattttttgg ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta
    12241 cgacttgata attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa
    12301 aagttgcgac cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat
    12361 gacagacaac agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt
    12421 aacagcactt ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg
    12481 ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa
    12541 agtagctgct caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga
    12601 tcgtgtattt gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt
    12661 gatgtgcaac aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc
    12721 cgattatgta gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca
    12781 catgcttcgt ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca
    12841 agaacagctt tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc
    12901 aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt
    12961 tattggtgaa aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa
    13021 tatggtcact cttgatggtg aagtcaaagg acaacatgct ggcttgatat attatacgat
    13081 tggtcaacgt caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt
    13141 tgtaggaaaa gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc
    13201 attgtatgcc acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc
    13261 aaaagaattc aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt
    13321 tcgcttattg gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtgcaatcac
    13381 tcctggacaa gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga
    13441 ccacgcatat caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa
    13501 acagctatgg ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca
    13561 ataaaattaa tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc
    13621 caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat
    13681 cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg
    13741 acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt
    13801 gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca
    13861 tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa
    13921 aagcgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta
    13981 ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt
    14041 gcaacgatgc tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat
    14101 gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat
    14161 ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta
    14221 ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt
    14281 tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg
    14341 cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt
    14401 gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg
    14461 ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa
    14521 gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga
    14581 aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg
    14641 tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg
    14701 aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca
    14761 atcttgacta atctattctt attgactttc ttagttactg attatgccta tcaatttgcc
    14821 tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc
    14881 cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt
    14941 tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc
    15001 tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg
    15061 ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg
    15121 cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg
    15181 tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc
    15241 gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt
    15301 tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt
    15361 caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa
    15421 tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt
    15481 tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc
    15541 tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga
    15601 agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg
    15661 cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc
    15721 atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat
    15781 cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc
    15841 aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta
    15901 tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt
    15961 gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat
    16021 tttaaaacaa tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc
    16081 tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt
    16141 agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg
    16201 actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt
    16261 cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc
    16321 agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac
    16381 tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac
    16441 aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccac gataaccaaa
    16501 tgcactggaa acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga
    16561 ttggttataa ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc
    16621 ttggacagca gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa
    16681 ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc
    16741 aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc
    16801 tatcaaagtg gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc
    16861 atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga
    16921 tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc
    16981 tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg
    17041 gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt
    17101 agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc
    17161 tgtaacagaa ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc
    17221 agttgcgaca agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg
    17281 caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat
    17341 tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca
    17401 ccaaggagag ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa
    17461 gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat
    17521 gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg
    17581 ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc
    17641 ttctttctga aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca
    17701 atgaaagtct cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt
    17761 ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact
    17821 gctttcgaga actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca
    17881 aacgcttgga tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca
    17941 aaacagcgct aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg
    18001 tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt
    18061 tttcaaatca agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt
    18121 tattagatgg aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat
    18181 taacgaaaga agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc
    18241 tttccttacg ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct
    18301 atttttggat gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta
    18361 acaattttaa gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt
    18421 gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt
    18481 aattaccatg ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat
    18541 tgaagaagaa gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg
    18601 gaacgaaatc catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt
    18661 ttggaatgac ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa
    18721 gggacttgcg ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta
    18781 tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg
    18841 gtgctaatca aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc
    18901 ttggtcattt attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc
    18961 aaatggacac gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag
    19021 gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca
    19081 ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc
    19141 atttcaataa accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa
    19201 ctggattcaa gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg
    19261 cccgggcaaa agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt
    19321 acgcggataa tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc
    19381 aagaaaagaa aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg
    19441 gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag
    19501 cacaaaaact tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa
    19561 aattaaaaac ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca
    19621 atcaacgcta tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga
    19681 cccataagat cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa
    19741 gtacgatgaa agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt
    19801 tgaaaaacgg agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc
    19861 accatgcact tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc
    19921 gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag
    19981 tcagccaaac aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc
    20041 gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg
    20101 aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta
    20161 aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag
    20221 ctacgcgtgc agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc
    20281 aaggactgca agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag
    20341 taacaccaga tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga
    20401 aacaagcagg aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga
    20461 tcaaatcaag caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat
    20521 gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt
    20581 cagattgcaa ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat
    20641 gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg
    20701 atggaacgac agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg
    20761 aagaacgacg cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag
    20821 cagcagcact aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag
    20881 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa
    20941 tttcggctta tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg
    21001 tgacaagact tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta
    21061 tattattggt gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa
    21121 acaggacgct gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga
    21181 cataacaatt ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat
    21241 gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg
    21301 acacaagaca aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg
    21361 ataccatcaa tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca
    21421 cgttcgcggt tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct
    21481 tttgaaaatc ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc
    21541 aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg
    21601 tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg
    21661 gtgccgacac aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg
    21721 ggcggatctt gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat
    21781 ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa
    21841 ggataagctg aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca
    21901 ataaacaaat attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga
    21961 ggaacagttt tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc
    22021 ctttcaatcg tatgtttata tatggtttac attcaaaatt taataaacct taaataacac
    22081 ctttaaaatt tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag
    22141 tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa tttttattag ttggcaagta
    22201 gttttggata gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa
    22261 aatacacaca cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt
    22321 atcttttcag ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca
    22381 agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat
    22441 aaagagaggt acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg
    22501 agaaatccga acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc
    22561 gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat
    22621 cgggcaaaat ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa
    22681 acgccaattg ttgttgttag aaggatatct gcaaaattaa aaacagttgt gattcggaca
    22741 ttgttccatg cgagaatcac aactgttttt tcatgttcat tctaaacttt gatcagccaa
    22801 ataaatagta aagccatcac tgtttggaga agcccgacag ctaatccata agataagaag
    22861 cgtgtacctt ctttcatgaa ttttgcaaag tctaaacgca gaccaattgc cgctaaagca
    22921 gtgatctcaa accagctgct gataaaatgt gccgattggt caataactac aggtaaagaa
    22981 aacaagctgt tgaccaaaca aaagatcaca aaccctaata cataccaagg aagcggtaat
    23041 tttcgttttt tctgcgtatc agttggcgtt tcaaacagag ctggttgttt cttgtgcatg
    23101 aatttttcaa acagatagac aacaacaacg agcatcataa tacgtagaat cttaaatagc
    23161 atggcgaatt cgacaacaga attgtcaagt aagcttgccc cagcgactac ttgaccaaca
    23221 gattggagtg taccacccaa aagtgcactt ttttcaatga gatttccacc aaacaactgt
    23281 aatccaagaa aagggagagt caacatcatc acagtaccca gtagattgac tagtgtgatg
    23341 atctgtcctt tttcttcctc tttcgcatgg atggaagggg cgattgaagc aatagcagaa
    23401 gaaccacaaa cagcgtttcc tccagccatc atcaaagaag aagtatcgct gaattgcagt
    23461 tttttaccaa tcaagtaagc accgatgata gtgactgaca tttgcagtaa cacaaaaaca
    23521 attcctttta caccaatttg agcaatcgtt tgagctgtga cagttgcacc tagtaaaaaa
    23581 acagagaatt ctaatagttt gctttcagca aattttgtgc cgattgctag aacaggctgt
    23641 ttaaaaaaag tatttcctaa taaaataccc aataaaatag caatagtcgc tccacctaac
    23701 tgtggcagat aaatagcaag aagcctgctg aaggcagcga tggcaaaaga aagcagtaaa
    23761 ccgggtaaga tttcgcggac cgctgaatcc tttacagatt ttattggatg tgacaatgat
    23821 ttcatttgat cggttccttt cgtcaataaa tactaaaagc atactcgaac atagtagcac
    23881 taaaccagta ttttgacaaa agaacaagag gtcgtgacaa aagtcttgta cgacctcttg
    23941 tttcgaataa acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
    24001 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttcttttac
    24061 gacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccagtaca
    24121 aaaacatgaa cagctgtttt tgtttggccg ttcttattac ttgatgcaga acagcttttt
    24181 cacaacctat tgaatcgatt attaagcgat ttttttagaa acaaattttc catgtttgct
    24241 atgttcttta attaagaaac gtacgtaatg agcaagaacg tagaagacaa ctacacttgc
    24301 aaacagacat tccgcaactt ttaagaaatc caatacgcta ctatagatac ctaaactgtt
    24361 gaagtaatga acagtagtaa atatagctgt tgcagagata acaagcggga atgtaaaggc
    24421 tgcatagctt ggataaaaac gaagcttgaa caaacgtgga agcatagaca gcacaacgaa
    24481 atacaatact tgagataaag cgaacaatgc tacgacaaga taaaaattag gttcagcaaa
    24541 gtttttaaga taacctgtta ggcaaagaga accaggtgct gcaacgatcg taatcagcgg
    24601 acgtgtcgga tgagccatat ttcttacgaa aaagacacgt ttgatgatga tcggcaataa
    24661 gaacgcatag cagatcaatg aaatccaaaa gaaaattttt ccaataagtg ggaagaagtt
    24721 cccggcagtt acagtgatga ccccaaatcc aacgtaaatg ataaaccagc ttggataaat
    24781 tgcttcaatt gaaagatcag ctttgataac atggtgataa ttgaaataaa agacaaggat
    24841 cacttggaag ataacagcgc acagccaaat gaatttgaca attggtgcaa tactttcata
    24901 tgaaacaaaa taagtacaaa taaccatgag tgacatcgta aaagttggtg aaacggaggc
    24961 gatgattgga tcatgcaacg tttgtttcgt gtgttcgaat aagataaaca atttaacaaa
    25021 aatgaatatc ataagaatca tagagatacc gccagttaga ttgcctaaag caacatggtg
    25081 atagtcttta aacagatttc caagggaagc taacccaagg atcaacccgc agatcgggat
    25141 cggaataatt ttcaaaaagt tcttcatata taatattctc ctttttattt aatagatttt
    25201 agtaccaaga tgaacgaata cattgtaaaa tgaataggaa tgtatgatta cgagcaagca
    25261 gacagctgat tgtcttcatc taagttacta gttggatcag caactgtttt gttgtacatt
    25321 tctattatag atggtttttt gtatatttaa atcaaatagt ttttatagta attatcataa
    25381 aaagttatgg aagtggtgcc atgtttaagt tattaacgac ttttcgagca gtatatgaaa
    25441 caagaaattt ttccagagca gcagaagtac tgtttttatc tcaaccagca gtctcgaatc
    25501 aaatcaaaca gttggagcgg gaattgaata tcgatctttt tcaacgtaac ggaagaaaag
    25561 agatgcttcc aactaagcaa gccgacttat tatatgaacg gacactggtt ttgttagaag
    25621 aatgggaaga aactcagcaa aaattgctca atgaacaaaa tgaagaagaa gtatgtcgga
    25681 tcgttgcttc ccacaccttt gctgtctatc ttttgcctca gttgatgaaa catctgatca
    25741 aaaggtttcc taaagtgcat tttgagatcg aaatcacgaa ttctcatgaa tcattagaac
    25801 aagtagcgaa acatgaatcc gattttggtt ttatcgaaaa gccattagag acttccggcg
    25861 tacaaagata ctctttgatg gaagaccagc tagtcatcgc tggggaccca aaaagcaaat
    25921 tgtggcttgt aagagaagca aactctggtg tttttcatta cacgcagcgt tattttgaag
    25981 agatgaatat ccaagataag aagatgatcg tgaaaagtaa cgatgtaatt gttgcgttgc
    26041 tgaaggaagg aatcggtcgc tcgattttgt ctaagcgctc tgttccagaa aatgtgccaa
    26101 tgactccgtt gaatttccat cgatattttt atttcatcca acgaaccaat ctggtttctg
    26161 atcagctgct agagattgcc gaagagatcc ttcgatacaa tgaaagcaca gaaacttctc
    26221 acagcgagga gaatctcagc tggtagataa ttgcatgtat caaaaaaagt tctatgatac
    26281 actaaagata agcagtaggc ttttgtactg catttgctca agcaactgca caaacggaaa
    26341 gttaattttt cccaaatgtc tatggaaaga ctttccatta tctaactgga ccgctataat
    26401 aatcagcagt atggttttta agaaaaggga tgaaacatgg aaaaaaaatt aaaagcttcg
    26461 aacgataatc gtattgatta tggcgtgatt ttaccagtct ttctgttatg tttgattggt
    26521 ctgctctcat tatatgtagc acttagccat gatccgaaca atccagcagt cttgaaaggc
    26581 gtagggatgc aagttgtctg gtatttagtt ggagcggcag caatcgttgt catcatgcat
    26641 ttgaattcca aatggatctg gaaactgact ccttaccttt atggtttggg gttagtggta
    26701 atgggactct tgctcaaatt ctatgatcag aatttagctg cttcaacagg gtctaaaaac
    26761 tggtttcgat tcggtgcgtt tactttccag ccagcagagt tgatgaagat tgcgtacatc
    26821 ttgatgatgg cactgatcgt aactaaacat aatacgcaag taaaagaacg gacgatgaaa
    26881 tcggactttt ggctgattgg gaagctcttg atcgtgacaa taccggtttt agcattgatc
    26941 atggcccaag atgactttgg aacgatgctt gtctttcttg cgatctttgg cgggattttc
    27001 ctgatgtctg gaatctcttg gcgcatcatc gcacctgtag taatcttggc agtagtcgtt
    27061 ggagcaggaa cgatcttctt ggttacgact gaaggcggaa gagacttgct ttataaagtt
    27121 ggattcaaat cttatcagtt tgcacgtatt gattcatggc tggacccttt ccatgatacg
    27181 tcaggaatga gttatcagcc ggcccaagga cttttggcaa ttggtaccgg tggcttgttt
    27241 ggtaaaggat tcaacgtaag caatatctat gtgcctgttc gtgagtccga tatgattttt
    27301 accgttatcg gtgaaaactt cggctttatc ggtggtgcat tcgtcatttt cttgtatttt
    27361 attttaatct atcgtatgat tcgggtgtgt ttcgatacga ataatgaatt ttacgcctat
    27421 atcgcaagcg gtttgatcat gatgctgctg ttccatgtct ttgagaatat tggagcaaat
    27481 atcggattac ttcctttaac agggatccca ttgcctttca ttagtcaggg aggatcctcg
    27541 attttaggga acatgatcgg tatcgggttg atattatcga tgagatacca aaatgaagca
    27601 cctgcaagat cttcaaggag gtaagaaaat tgtacacctt ttactggtac ccgaaatgtt
    27661 caacatgcaa aaaagccaaa gcgtggttag accagcatgg cgttagttat caaaccgttg
    27721 atatgatcaa agaagtaccg aaggcctcaa cgattgagac ttggctagaa acgagtcaag
    27781 taccaatccg tcgcctgttc aacacaagcg ggatgaaata tcgggaactt ggactgaaag
    27841 atcaagtagg cgagtttacg gcttcaaaag cgagccaagt cttatcgaca gatggtatgc
    27901 tgatcaagcg accactgatc ttacgagagg accagcttgt tgcaattgga ttcaatgaaa
    27961 aaatttatga aggagaactg atataaaatg gcaggcaagc atttgaagaa aaaggacaac
    28021 ctttggatct tatttaatgg aaaagaatac tgtgttggtt tgacaaatga agcgcaagaa
    28081 gaacttggag acatcacgtt tgccaatctg ccaaaagcag gacaatcgct aaaaaaagat
    28141 gaacctctga tcgaagttga agcggaaaaa gcggtcaatg aatttgccag cccgctaacg
    28201 ggtacagttt cttctgttaa tgataaaatc ggtgaagata tcaatatctt gaatgattca
    28261 gatgaaatga atgcgtggat cttaagtttc aaagatgttg atccaaaaga atttgacgaa
    28321 ttataaaatc agaaaatcac ttgaaaaaag ttgacactga ttcttccagt tgctataatc
    28381 ataagcaatc ctatatgaaa cgagaaagct ttgaaaagaa gagtacatgt cacggacgtt
    28441 tgaagagagc cctgtttgct gaaaatgggt aacgaaaatg agatggaaga tggtcttaga
    28501 gcagagtaag cgagcgtaag caaacttact acgggagtgc ccgttacagc gcgacagtat
    28561 acggaaagaa gctaagtcca agtactggaa gaggctgtta ttgcgaagta acggtaaatc
    28621 aaggtggtaa cacggaagtt caagctttcg tccttttgct taaggcaaga gggcgaaggc
    28681 ttttttgttt gaaaaatcat aagagaaaga tgtgaatggc atgtcattaa tccaactaac
    28741 gaatgtcaaa aagacattca atgggaaaaa cggaaacatc caagccgtca atgacgtttc
    28801 actaaacgtt gaaaaagggg atatttatgg aatcgtcggc tattctggcg caggaaaaag
    28861 tacattagtc cgtctcctga atggacttga gcttcccacg agcggcgaag tgatcgtaaa
    28921 cgatcaagat attacaaaat taaaaaacaa agaattacgt acattccgaa agaaaatcgg
    28981 tatgatcttc cagcatttca acctgttatg gtcaagaact gtacttgaaa atatccagct
    29041 gccgctggaa ttagctggcg taccaaagag caaacgtaaa gaacgggcag aagaactgtt
    29101 gcgtttagtc gggttagaag gacgcggaca agcatatcct tcacaacttt ccggcggaca
    29161 gaaacaacgc gtcggtatcg cacgagcatt ggcaaatgat cctgagatcc tgctttgtga
    29221 tgaggctact agcgcattgg acccgcaaac aacagaagaa gtattagact tattgttagc
    29281 gatcaataag aaattgaatt tgaccatcgt cttgatcaca catgaaatga atgtgatccg
    29341 caaaatctgc aacaaagtcg ctgtgatgga gttggggaaa gtcgtagaag aaggagacgt
    29401 attgactgtc ttcagacaac cgaaaaaaga agtaaccaaa cgctttgtac aacaagacat
    29461 cgaaccagaa gctgattcga cagaactatt agctgaactc atcaaagaaa atccaagcgg
    29521 ccgtttggca actttgacct ttagcgaaag aaatgccaac gagccagtca tttcccaagc
    29581 gattcgacgc tataacatcg atgtcaacgt tgtgtatgga aagatcaaac aagtgaaaga
    29641 aggttccttt ggttcattga ctgtcttgat gactggtgaa accaaagaat tagatcaagc
    29701 agaagtgttc atcaaagaac aaggagtagg aattgaggtg atccatcgtg gataaaagct
    29761 tttcagaaac atatctagat tttaaacaaa tcaacccgga aacgatgaat caggctatcc
    29821 ttgatacact atatatgaca ttaatctcaa tggtcttagt gacgatcatc ggattagttt
    29881 tgggattgat cctttactct attggacgca aaaacaaacc gtatgctcga atcatttatg
    29941 gaatcgtatc gatcatcagt aatattttcc ggtcaacgcc atttatgatc ctgatggttt
    30001 tgatcattcc atttactaaa gcaatcgtag gcactatgct aggtgcgcaa gcagccattc
    30061 cagctcttgt tttatccgca gcaccatttt atgcccgtct agtagagatt gcttttcgtg
    30121 aagtcagccc tggtgtactg gaagcagcag atgcaatggg agcaagttac tgggaaatca
    30181 tctggaaggt cttgatacct gaaagtattc ccgcattgat ttctggatta acagttacaa
    30241 cgatctcgat gatcggattt actgcgatgg ccggcgcgat cggcgcaggt ggacttggag
    30301 gtcttgcttg gcaggaaggc taccagcgtg gtaatctaac agtgacattc gttgcgactt
    30361 taattatttt actaatcgta tttatcgttc aaggaatcgg tgatttctta actaagaaaa
    30421 cagacaaaag ataataggat atagggggat gtaggatatg aaaaagaaaa tttttggatt
    30481 tgcagcagcg cttttgctga ctgtaggatt agcagcatgc ggtaacaaca gcgacagcaa
    30541 agattcagca aataaagcag acgacacgac attgaaagtc ggcgcttcac cgacaccgca
    30601 cgcagagatt ttggaacatg tgaaaccatt attgaaagat gaaggaatcg atcttgaaat
    30661 cgtcaaattc gatgactatg tattaccgaa ccaatctttg gaagaaggcg acattgatgc
    30721 caattacttc cagcatattc cttatttgaa caaagaaatc aaagaaaaag gatatgactt
    30781 tgtcaatgcc ggtgctgtcc acatcgaacc aatgggcttg tactctaaaa aagtcaaaga
    30841 tgtttccgaa ttaaaagatg gtgcaacaat cattacttca aattctgaat cagactgggg
    30901 acgcatcatc acaattctac aagatgctgg cttagtcaaa gtcaaagatg gagtagatct
    30961 ggaaacagca acattcgatg atattgaaga aaaccctaag aacttgaagt tcaaccatac
    31021 gatcgaccca tcacttttag cttctacgta ccaaaatgat gaaggtgacc tagttgcaat
    31081 caacgcaaac ttcgcttacg gcgcagggtt gaatccatta gaagatgccg tattgcttga
    31141 aaaggacaac tctccttacg tgaatattat tgcgactcgt aaaggtgatg aagacagtga
    31201 caaaatcaaa aaactgattg aagtgcttca ccgcaaagaa gtccaagaat ggatcacaga
    31261 caaatggggc ggctctgtta aaccagtaaa ggctgatgca aaataaggta tggaacaaaa
    31321 aagatgagaa ataagtctgt aatggcttat ttctcatctt ttttatacct attctaattt
    31381 agaaatcaaa attgtttaat aataaataaa agatatttga ttttagtaag gaaggtcatt
    31441 gcgcttcatt ctcaattagt ctaaaatgag tgagaacgaa agagaatttg ggggtataat
    31501 gcatgtctgt cttagaaata aaaaatctac atgtgtcgat cgaggaaaaa gaaatcctta
    31561 aaggcgtaaa cctaacaatg aaaaccggag aaatccatgc catcatggga cctaacggca
    31621 caggaaaatc taccttatct gccgctatca tgggcaatcc aaactatgaa gtaacagaag
    31681 gcgaaatttt gtttgacggg aaaaatgttt tagacttgga agtagatgaa cgtgcgcgac
    31741 ttggcttatt tttagctatg caatatccaa gtgagatacc aggtatcacg aatgcggaat
    31801 tcatgcgtgc agcgatcaat gcgaaacgtg atgaaaacga caaaatttct gttatggcat
    31861 ttttgaaaaa gttagatgaa aaaatggctt tactaaatat gccagaagaa atggctgaac
    31921 gttatctgaa cgaaggtttt tctggcggag aaaagaaacg gaatgagatc ctgcaattgt
    31981 tgatgttaga acctacattt gcaattttag acgaaatcga ttcaggtctg gatatcgatg
    32041 ctttgaaagt cgttgctaaa ggagtaaatg aaatgcgcgg agacaatttc ggtgcattga
    32101 tcatcaccca ctatcaacga ttattgaact atatcacacc tgatgtcgtt cacatcatga
    32161 tggaaggccg agtagtcatg acagggaatg ctgacctagc taaacgtctg gaagcagaag
    32221 gctacgctgg tatcagtaaa gaattaggaa tcgactataa agaagaagaa gcgtaagggg
    32281 gagcaagaat gaaagaaaaa aactggctcg attatcttga cgcggtaaat gatttttctt
    32341 taagtaaagg cgaacctgat tggatgagaa ccttccgtca agacgcacta gcgaaagcag
    32401 atgaactgcc attgccgcat atcgatcggg tgaaattcca tcgctggtcg ttatttgatg
    32461 tgaaagaaac acaaaccatc agtgaaacag gcacgattcc ggcatttgat gcaatgaaag
    32521 acaatcctgt gcttgtccaa caaggttcat ggacgatttt tgaacaactg cctgtggaat
    32581 tagcagaaaa aggagtcatt tttaccgact tgttcactgc aatgatcgaa tatccagaac
    32641 tagtccaaga atattatatg aaaaaagcag taaacatgaa cgaagaccaa ttgactgcac
    32701 tccatgtagc ttttatgaac agcggtattt tcctgtatgt gcctaaaaat gtcgtgatcg
    32761 atgaaccact cgagtcctta ttcattcaag atggcgcatc cgatgagcac ttcttcaagc
    32821 atgtgttgat cgtggcagat gaacatagtg aattttctta tctggaacgt ttccagacaa
    32881 ccaaagaaca agtagcaaaa tcatcaggta atatcatcgt ggaagtcatc gctaaagctg
    32941 gttcaaaaat caaatattca gccgtagatc aattaggaga aaatattact tcttatatga
    33001 atcgccgtgg gcacatttta cgagatgctt ctgtcgactg ggcaattggt gtcatgaatg
    33061 acggtcatgt gatcgctgac tttgactcag atttagcagg agaaggtgca cacgcagaag
    33121 tcaaaatcgt ggcaatcagc tcgggcagac aaatccaagg aatcgataca cgagtaacca
    33181 ataaagcccc tcatacgatc gggcatattt tgcaacacgg agtgatccga gaaaaaggaa
    33241 cattgacctt taatggtatc ggccacatct tgaaaggggc aaaaggtgca gatgcccaac
    33301 aagaaagtcg tgtcttgatg ctttctgata aagcacgtgg tgatgcgaat cctattcttt
    33361 tgatcgatga aaacgaagta actgctggac atgctgccag tgtaggacga gttgatccag
    33421 aagaaatgta ctatctgatg agtagaggat tgcataaaga agaagcagag cgcttagtga
    33481 ttcgaggatt cctcggttct gtcttaacag caatccctgt agaacaagtt cggaaagaat
    33541 tagtcgaagt aatcgaaggg aagttgaatg gatgatcgat gctaaaaagc tacgcaatga
    33601 ttttcctatc ttatttcaag acgtcaatga tgaaccttta gtttatctgg ataatgcagc
    33661 gacgacccaa aagccaaaac aagtactcca ggcattagcg tactattacg aacacgataa
    33721 cgccaatgtt catcgcggtg tccatacgtt agcagaacgc gcgacccatg attatgaaca
    33781 agcaagagaa aaagtacggg cgttcatcca tgcaaaagaa acagcagaag tgctgtttac
    33841 tcgtggtacg acgacaagct tgaattgggt agccagaacc ttcggcgacc gctatatcaa
    33901 agcaggagac gagatagtgc tctcttacat ggaacatcat tctaatgtta tcccgtggca
    33961 gcagttggca aaacgaacag gtgctgtctt gaaatatatc gaactaacag aagaaggctt
    34021 cctggatatg gaggatgcag cgaaaaagat tacggacaaa accaaaatcg tctccattgc
    34081 tcatgtatcc aatgtcttag gtgtcttcaa tcctgttcaa gaattagctg aacttgtcca
    34141 tgaaaacggc ggggtcatcg tagtagatgg tgctcaatct gccccgcaca tggccattga
    34201 tgtccaagca ctagactgtg atttctttgc tttcagcgga cacaaaatgt gcggaccaac
    34261 aggtattggt gttttatatg gtaaacgaaa atggttagaa gaaatggaac ctgttgaatt
    34321 tggtggcgaa atgatcgatt ttgtcgaatt atacgacagt acatggaaag aactaccttg
    34381 gaaattcgaa gcagggacac caaatatcgc cggcgctatc gcattaggcc atgcaatcga
    34441 ttatttgcaa aaactcggta tggacaatat ccatcgatat gaagaagaac tagccgctta
    34501 tgtgttgcca aaattacaag caattgatgg attgactgtt tatggtccac aagacccgaa
    34561 ttttcggaca ggagtcgttg cattcaattt ggatgggtta catccccatg atgtcgcgac
    34621 agctttagac atggaagggg tagccgtcag agcaggtcat cattgtgcgc aaccgctatt
    34681 gaagtacctt gaagtatcag cgactgctcg cgcgagcttt tatttctaca acacaacgca
    34741 agacgccgac cgtttagttg aagcaatcat ggcaacaaag gagtttttcc aacatgggac
    34801 tatctaaatt agacaattta taccgtcaag tgatcttgga tcattcttcc catccgcatc
    34861 atcgtggaag cttgactgac tcgacaagca agatcgaatt gaacaacccc acatgtggag
    34921 atgtgatcga attgcaagtc gatgtaaaag acggaatcat caaaaatatc gcctttgacg
    34981 gcagtggctg ctccatcagt acggcaagtg cctcgatgat gacagacgcc gtcatcggaa
    35041 aatcaataga agaagcagaa catttaactg tagactttct acagcttgtc caaggaaaag
    35101 aagtcaaaga tcccgaccaa ttaggcgatg ctgctatgct tggtggtgta gctaaatttc
    35161 ccgctcggat caaatgtgcg acacttgcct ggaaagcact aggaaaagca atggaaaacg
    35221 atggtaccgc agctttccat cattaccaca aggaagagga gtgagaacat gggcgtacca
    35281 gaattagaag aatataaatt cgggtttcac gatgatgtcg aaccggtttt cagtacagga
    35341 gaaggcttga cagaagaagt gatccgtgag atgtcaagaa tcaaaggcga accagaatgg
    35401 atgcttgaat tccgtctgaa atctctagaa acatttaaca aaatgccaat gcaaacatgg
    35461 ggccccgacc tttcagacat cgatttcgat gccatcaaat attaccaaaa accaagtgac
    35521 aaacccgctc gtgactggga agacgtacca gaaaaaatca aagatacatt tgaaaagatt
    35581 ggtataccag aagctgaacg agcttatcta gccggtgctt ccgcacaata tgaatcagaa
    35641 gtcgtgtacc ataatatgaa agatgaattc gaaaagttag gaatcgtctt tactgacact
    35701 gactctgctt tgaaagaata ccctgaatta ttcaaagagt acttttcaaa acttgtaccg
    35761 ccaaccgaca acaaattagc agcgctgaat tcagccgtat ggtcaggtgg gacctttatt
    35821 tacgtgccaa aagccgtcaa agtcgatgtt ccattacaaa cgtacttccg gatcaatgcg
    35881 gaaaacacgg ggcaattcga acgtacgctg atcattgttg acgaaggagc aagcatccac
    35941 tatgtcgaag gttgtacggc acctacttat tcaagtaaca gcctgcatgc cgccatcgta
    36001 gaaatcttta caagaaaaga tgcgtattgc cgttacacaa cgatccaaaa ctggtcagac
    36061 aatgtgtaca acttagtaac caaacgagca aaagcatacg aaggcgcaac cgttgaatgg
    36121 atcgacggaa acctaggtgc aaaaacaacg atgaaatacc caagtgttta cttagatgga
    36181 aaaggtgcac gaggcaccat gctctccatc gcctttgcag gagcaaacca aatccaagat
    36241 acgggtgcaa aaatgatcca caatgcacca aatacatcta gctctatcgt ctctaaatca
    36301 atttcaaaag acggcggaga agtaaactat cgaggacaag taacctttgc caaaaacagt
    36361 gcgggttcga tctctcatat cgaatgtgac acgatcatca tggacgaatg gtcgaaatcc
    36421 gatacgatcc cattcaatga gatccacaac agccaagtct ctttagaaca cgaagccaaa
    36481 gtctcaaaaa tctcagaaga acaactctac tatctgatga gccgaggcct gaccgaatct
    36541 gaagcaactg aaatgatcgt catgggcttt gttgaaccat tcaccaaaga acttccaatg
    36601 gaatatgcag tcgaactcaa tcgattgatc agttatgaga tggaaggcag tgtaggataa
    36661 acttgttata ggagctagaa tccttggtat ataagggttc tagctttttt attttgtcta
    36721 tatactagaa atgaccagct tttgaccagc tcttttactg atgagccatc atatcagcaa
    36781 agtcttgacc tgttttctta ggtgcattat ttgtaatatg tgcatagatg tctaatgtca
    36841 ctttactatt ttcatgccct aatcgttgtt gaactacctt gacagattct cctgcttcta
    36901 ataataaact agcatgtgta tgtctaaacc catgaggtgt aatacgaggc aagttatatt
    36961 ttttcaaaat atatcttaac caatcattaa cagtgtttgg aatgtataga gtgtttttaa
    37021 ctgttgtaaa aacatgttgc tctttagagc tagtattgta tccaagtttt aaatactctt
    37081 ctttttgctg taatttccag ttgttgagga tagaaagagt ctcagtatct aaagagataa
    37141 ctcgtctaga agctcttgtt ttcggagatt gaattataac tttaccaaat tcatctacag
    37201 caagagtttt attgacatgt agttctttat taaacgtgtc tatgtctttc cactgtaaag
    37261 cgagtacttc actctttctc atacctgtgt atgccaataa tctgaagaag gcgtattgtt
    37321 tcatgttacc aaaatcttta aaagcatcca gtaaattatt aagctcctct tttgagtaaa
    37381 atttacgatt tttctcatac tctttttttc gtggaagaag tgttttttcc ataggattat
    37441 tgtcaatata ttgcattgca accccgtagc gtaaaatttg tgcagtctgt tttctcatat
    37501 aatcgtaact ttcatattta ctgtgccatt catctaccac tttttgacaa tatgggacag
    37561 agatattcga tagtttaagc ttaccaaatg ctggaagtat ttgattattt gcgtatctta
    37621 ctgagattgc gattgttgaa gctttaacac tggtacgctg atgagttatc cattcattat
    37681 acaggtcttc aaatgtcata tcaggtgctt tctttttaac accgtataat atttgatgtc
    37741 tgtactcgtc ataatctttt tgagcttctt gtttgctact aaaacctctt ctcgtagtat
    37801 ttattttttc accagttcgt ggatcggtgg caatgtgtcc tttatacatc cacgccactt
    37861 gaccgttttt ctttttgtac tttgatattt ttgccatgaa atcatctcct cttagtttac
    37921 aaggagtact taccactaga tggtatcacc tcccttcatt tttttcttag tgtatttttt
    37981 cccaattgat gaaatcgttt gttttagctt gtttatatta tcaataattt cgtttacagc
    38041 ttcttcggga taattcatgt cttccacttg actatgtatc ccttcatcta tcatttcttc
    38101 tagttgttcc tctatactgc tagtgagcag aagaatgtta ttgtcaaatt cttctataat
    38161 agcattgatg aataattctt ctatttttaa gatattgaaa agactaattt tttcattttt
    38221 aactttatta caagtcttgt tagcaataaa ttcttgcatc tctggttcta atttggaata
    38281 tctatccagc catgtatcta aattagagtg tactggtggg aactgttgac gaagatgctg
    38341 gaacaggttt ttatctggaa atgttatacc atctagtaca atgctatctc tataaagaat
    38401 aattcccaaa atatattctt ctacattgcc acaaactaac tcctctatac tcatatttcc
    38461 caaatcagca attcgggata atcgctctgg tattggtaaa taggttccct tttcccattc
    38521 gtagatagtt tttttgtcaa tcaattgacc attgttaatc ataagaccaa atgctggttg
    38581 tgttaatttt ttactctttc ttatagcttt tatttttgtt ccgagtatct tttttcgttc
    38641 taaataattt attggtggac gctttatcat attgaccaca cctcctaaaa ttagtatacc
    38701 tcaaaaaggg ggtgttgtaa ataaattcac acaacatatt gacacccctt ttgtatcgag
    38761 gtattatgtg tatgtcgatg aactgataat cattttttat ttgattaatc ggttaataac
    38821 aacaaaatac ttttattttg atcaaataat acgaagatga ttcttagaaa atttgttcaa
    38881 gtaatcacga aaggaggggg aaatatgtct ctacaaatca ttgatagcat tgtttctata
    38941 caagttgaac aacaagtgaa agagataatt acagtgctaa agcctatgct agaagacatt
    39001 atttctggtg ttactctaga gtatatggac tatcagcagg caagtgaata tctaggtgta
    39061 tcagtaggaa cgattagaaa atatgtttct caatacgggt tacctgttat aaaaattgat
    39121 acggtcatta gattcaaaaa atctgatatt gatagtttca tggaacagta taaaggttga
    39181 gtaaacaaat aagactaaaa catatagtaa taaagagttt gtgtttatta tctgtatgtt
    39241 ttagtcttta acagaatttt ataaggagga aacacaaatg gaaacaccaa tgattaatat
    39301 tccaattaat gtagataaag aaagactatt agagaaacca gttatagagg atgaaaaaaa
    39361 gagaatacga aaaagatgtt tagataacta cagagaagtt acaagagatg agtttgtaga
    39421 gatactaaat tcatcacaat catttattcc aagtaagaca aagaataaag gcaacaaaag
    39481 tgaagagttt ttagaaacta gagtaattat tctggatgtc gataacactg ttaaagatga
    39541 gaaggggaag gtagtagatt taagtaaaga tgatagtaga tacttaagca tagaaaaggc
    39601 tttatctatc aatttagttc ataattctgc gtttgcgata caaaaaagta ttcggtactc
    39661 tgaaaatcta gaaaaattca agattgtctt tctattaaaa gaagcaatta ctgactatag
    39721 taaaatgatt gctgtttacg aatacttgaa aaaaatcata cccgggtgtg atgacaatgt
    39781 aaatgcttcg aatagaatgt tttttggagg atataagtca gatgcagtga taataattaa
    39841 tgaagacaac atgttagata ttactgagct tccgattgat tttaccatga catgttctaa
    39901 aaattgtcaa agtgattcag ggagagagta tgaactagtc aatgaaactg attttgttaa
    39961 gttgataaaa aatgacgaca aagaagaaat gaaacagtgg tttaaagact gcctgtttga
    40021 ttcctccgac atgtctttta atgagattta tgaaagattg ttaaccattg atatgaataa
    40081 attacttaag tcaagtaaaa acttaagatg tctgtttcac gacgaccata atccatctgc
    40141 tagtattttt accagtaaaa ctgggcattc cattttttat tgtcattcca gtaattgtgg
    40201 tatcagtgaa aattttattg gggttgtgat gttaattcta gagaaagata gtcgggtaga
    40261 aacatttaat tggctattag agaatctaga cctgtatcct acacattttc gaaaattgaa
    40321 agaggaatca aaagtacttt ttgacactct tgaatctatg gaagacaagc ttttcaggac
    40381 aataaataat tatctagagg aaatgcgacg tgtctatgat gccttgcttt ctgatgttaa
    40441 tttttttgat gatagtaagg aaagtgtgac ttgtatttta tcaggagaac aactagctaa
    40501 aaaaatgagt tcttactatg gtaaaactta tgacatagat aaatggaata agttactatc
    40561 atttatgaca tttataggat tgataaaaaa attagatgat gaagatgttc ctttgaagat
    40621 gctcgaatac ctaaataggt taaaggaaga gaaagcgtta ggaaacttta atcataagag
    40681 aagcaatgtc tatcgattag acgtttttaa tgcaacttct gtgattcaaa aattgaatga
    40741 agatatcctt ccacttctga aaaaagttca ttttacatat caatattttt cttatgaatg
    40801 ggttaagata tgttttaacg aaaaagaggc taaaagagta tttcctcaaa attttcggtc
    40861 agaactcagc aaggaaaaac aattgatttt agatgaggca catgaagttt tacaggactt
    40921 atcgtggcaa gggacatatc taatgacaga gaaagaactt attaacactg tttgtgaacg
    40981 tctacgtaat gttgggaata acagcgtgga aaatgcttta aaagaaaaca gaggctactt
    41041 tgttaaacaa ggattcactc ttgaacgagc ttctaaggat attaagaaac tatacaatgt
    41101 gaaagcaagc agtagttttt tagtctacct agcaaaagga gcagggatag atgaagtaat
    41161 aaaagaattg atagcatctg gtgttgaatt agttcctctt tatcaattta caagtgtaac
    41221 aagagatgat ggacggagag taaggaagag aaaagaccca attgtattaa actagacagg
    41281 ttatccatag ctcttaatct tatttcatct caatacaccc accgttaaag ttcggtgggt
    41341 tatttatttt tcaatctttc aatagctgat tgcgttcatt ttcttgcctt tttaatagct
    41401 tcatatagtc aattgttcga actgcatctt cagtgctaag agaacgatag agagccaaaa
    41461 actcttcctc ataatctacg acaacattgt ctagaaaatg gcttacaggg gttcctaggg
    41521 ctctagaaag agctgttaag ctatcgaggt taggctctgt tacagaattt tcccatcttg
    41581 ctactgcctg ttgtgtagag aagatttttt cagctaattc tttttgtgtc cacccgagag
    41641 cttttcgctt ttctctaatg acctttgcat attgtttctg tttcattaat acacccccat
    41701 ctcagtatag tacaacaagt tgttgtattt gtaatggtat ttgaagtttt tatattttat
    41761 gagttgaaat acatcatatt gttgtatata ataaaacagg aggggagaat catgaaaaaa
    41821 gtaagtaatc tcagccgata cagaaaagaa aaaggacttt ctcaaacgga gttagcaaaa
    41881 aaaatgaatg ttacacagca atgtgtaagt tcgtggcaaa cagggagaac gattcctaaa
    41941 ccctatcaaa tgaaaatgct ttctgagata ttaagtgtac caataaacga gctgttttct
    42001 gatgtgttta ataaagtaaa cagttaacta ttaagcaatt gaatgatttt aggaggagaa
    42061 gacatgtatt gtagaaaatg tggtaaagaa ataacagatg aaagtaagta ttgccagttt
    42121 tgcggagcat tacaagaaac agatgagaca actggagcag acgaagtaaa gaaaagtact
    42181 gagcagacat ttagtgaaat gaaggaacaa atcgtacatg gacaagagac agggaagctg
    42241 tatttgatta ttggttggat ttctatggta gtatcgttag catttattcc tgttgtattt
    42301 ggtgctgttg ctgttattat gggctactta tatcgagaaa aagatgagaa aatgggtacg
    42361 attttgatga tagcaggggt agctggagca atctttggtg tacttttagg gatgtcaatg
    42421 tactgataag agggataaga gcaaattatt ttggagatag gagataaaaa gttgaataaa
    42481 gtaaatatga acaagaagtt tgtcattata ggtatcgttg tcttggctat tataggtatc
    42541 attttttcaa aaatgacagg tacatctgga ttagagaagt catctaaacc attggtgaca
    42601 cagattttac aagaacagta caacttaaat aggtcatgtg atgatgtgac gattactcat
    42661 gaaaatggag ataacacata ccgggctaaa gctacgttag acaatggaag tgctatcaat
    42721 ataaatgtcg agtactatcc aaagaaagac cgtgtatatg tagagatacc atatactgag
    42781 gtgttaatgt taaattgatt taaatgtatt atgtataccg tccaaacagg aactcctaaa
    42841 ggtgtaagct tactaaggag tttttttgat tccctgtaaa taaaatacta atatagatga
    42901 gaaaaataat gttataataa ttgatggaaa ttgtttttaa agagctttaa aaattgggtt
    42961 cttacattgt tgattaaatg ctttaaaaga ggagctttat gatgaaaaag aattatttaa
    43021 aaacaggtat atttttggtt ataggaacta tactggttat tcaattatca ggttgtagaa
    43081 ataaagataa agatttctat ggatattggt caggaactgc tgagaaaatt cctgtagact
    43141 ataattttaa aataactaaa gacagagtaa tagaactaca aaaaaatggt gaaatggtgg
    43201 attttaagta tactctaaga cataaaaaat ttgattcaac tacatttcgt tcaatcatga
    43261 tatcaaaaga tgaattagtt aaagcgattc cagaattaaa ggaaaccacg ggaataagca
    43321 atataacgtt catgcctatt gacggtgaaa aagataaatt aactgtgttg gtaaatttgg
    43381 atgaacactt atcaacagcc actctttata ggactacgaa agatgagagt ccaattggta
    43441 aggtagaaaa gaaaaacgat acaatatcaa gacaaactgt agataaaaaa attgatacgt
    43501 tcataaaaac atacgcggct ataccaccta cttggaaact tgtaaatgaa gataagggaa
    43561 taacgataaa tactgataca gagattccga ctgatgctca atctttatca gatttattag
    43621 ctaatctaac aggagtcagt aatgcggtgt cagagcaagt cggtaaagat attccactta
    43681 ggttacttga aaatggaaaa aaagattatt ctgtggtatt taagaacgga aaggtaactg
    43741 attacaacga aatatttgag aaagtcattg aataatggtt tttctatctt tttattaact
    43801 accctacccc gttgtttttt gaccgtgttt cgagaggaat cattagaaag cataggctaa
    43861 ctaggggttt ctttttcttt tattttttgg gaatccgctt aagtgaaatg tatctgaaat
    43921 gaaacggaga taaagtgatt ttttgctgta atgacaaggt tttagctgtt tcagatacca
    43981 tgtaacccaa aatagtttat tgagaaaata ggagagtatt tgatactaac tagtagggaa
    44041 aaatgagata aaaagtaaat ggaatcattt caggtaatct gaaataaaaa aatatacagt
    44101 cctgtggcac tttacctatg ggactgtata ttttttactc tcagcgtttt ttgactccgt
    44161 gtctgactcc gttttattga ttttatatgt tattcagtga ccctttaaca ggtcaatttt
    44221 tatgtttttg tcttcgtatg actcagtttg atacctagtg atttccatgg taatacggga
    44281 tgttgaacaa gctagagaag ttctgcaggg gttaattgca aacaattcag ataaacaaat
    44341 tacaatagat tatttcaaga gggaaattaa aaaatcagag gaaaaatatt tagatttaaa
    44401 gtcggatatc ctattagcag acgatatagg ttcactaact gtcgaagaag atagcctatt
    44461 ctacgtctat tgtgcattga cttcgtaaaa gaagacgtct tttccaggta tacagttctt
    44521 ttatctactt catatgaaaa tttgggtaca aacgacaagg aagtcagttt tctggtcaga
    44581 ctatcgtacg aacatcttgt tgtggtattt acactcagct ttgatgatga gtacaagagt
    44641 ttttcagcga acaagacaga acatcgaaag aggtttccag acgaagtttt acaggctggg
    44701 ctgaagtatt atcaagaagg actcaccatt aagaagtctt tatcgagaat ataagaagat
    44761 acaggtatag aagtcagtcg gtcaacattc tgtgattacc taaacagcaa ggagtaacag
    44821 gtgaacggga aaaagagcac agggagggct atgttcactg gctgaagaag caagataaac
    44881 ctgtttatga cttgttcttt gaaataaaga aagctttaga tagtcagtca ttagagaact
    44941 tcattgatgg gtatgatgaa aagttatcta atgagcaaat gattgcgata atgaaagagg
    45001 tgtttcgtca gtatgggtta tcaaataaac gtaggaaaga ggtttttgac cacgttaaga
    45061 caataatgag cttttaccac acgaccctcg gctaaatcta aagagggcga aaaagggacg
    45121 agtggtaaaa tttaatggga atagctaaaa gtaaataata ataagtcaaa gactgcctag
    45181 tttggtggtc ttttttcatg ttcataaaaa taaaatagga cagacgaaca tcttcgctaa
    45241 ctcccctgtg ttgcaagagg caaactctat aattctccag ttaagctata ttttgttctt
    45301 tattttgaga tagaggaaaa aacaaaaatg tgttaaaatt gaacataagc ataattagaa
    45361 attgtcgagt tttttcaagt aaaaatcttg acttgtaagg aggaaaaggg catgatgaca
    45421 cgcaatcaca gtactagcca gcagtctgcc caaaagccca tatatgaaag tgataaccga
    45481 ttcgtagcat ccgtttcggt tatttgtgtt gggaagatgg gtgattttta gtggttgaag
    45541 atatggtgat ggttgaagag gaacaaggac gactaatcaa aagtaaaaaa cgtgtccaga
    45601 agcacggtga ggtatttaca ccgcaaagaa ctgtcaaaga tatgttggat ttgccaggca
    45661 taaaagaggc ttgcgaggag ctgcagacga ctttcttaga gccttcagct ggagaaggtg
    45721 ctttcttagt tgaggttttg actagaaaaa tggcaatggt tgctcgtgaa tataatgagt
    45781 ccctagaaca gtatgaaaat tactccttac tggcgctttc aactctatat ggcgtggagt
    45841 tactcgaaga caatgctcag acctgtgtaa tgaacatgtt tcaagtattc aacgatttgt
    45901 acagagaaca atcgaaaaaa catggtaccg caatgaaaag gaaagtccaa gacagtgcaa
    45961 agactttgat ttctaggaat atcaaacagg gagattttct tacaaaacga acaccagatg
    46021 ataatccgat tatttttagt gagtggacac ctaagacgct aagaaaagga cagaaaaaaa
    46081 tcactgtaaa tcgaactgaa tattctttgg aggatattta ttcaggggta acacgagagt
    46141 ttggagaggc atactcagct ctgcctgtat ttgaacagct cgatttgttt tcatttgatg
    46201 acgaggtcga tgaagaagtt tcaaatgatc caatattacg ttatgtagcg atgccgataa
    46261 ctgaggttta taaggaagag atggaggaaa ttgatgacga cagcgttgaa atctaaagct
    46321 aaatacttcg attacttacc taaatatggt tctaagctag aacacttcct tgggattcct
    46381 aatttagtag atgcaattaa cacgtaccgt gtaaccgtta atcaaatacc taatgaagtt
    46441 gctattggaa ctagacgtta ttcggaggta gcagcacgga agattgcttc aaatgtgggt
    46501 attgaccagc aaagtatgaa gtatgttgat gtacttcgcg agcttagggt cagaaataat
    46561 attactccta cgatagctga cttatttcat aagttgagaa aatatggaaa tgttgcagca
    46621 catgaaaatg gaattatacc tgtttcagag gcggtcgaat ctttagttac tcttgatacc
    46681 ttgctaagag tctttgcacg caatctgtct ggggataaat cgttaaaccc aaaggaagaa
    46741 attacggatg agatgtttgt tatcacctat aacacctttg accgtaaact tatttacatc
    46801 cagagtgcca aggatgctga cggcacatgg aaaatgtacc aaggactgga aaagattggg
    46861 gatgcatcag taccagagaa ttatgaggca gactttactc ctaatagcga gtatttaaga
    46921 gagtatgcaa gtagaagaat aaagcagtac atgacaacag cgggggtacc ttttgtcctt
    46981 cattgggcgc aattggctgt taataagaag aaccaatttt tccgtgacca tgacgtgcat
    47041 gaagtattaa agcgttcggg ctataatcct caaacgattg gcgaagatga cacagggaag
    47101 cctaatgagt ggttcagtgt aacagttgac gttgccaaga aagcaatcca agcggttaaa
    47161 gatggtaaag gtagtcttga ctcagaaaaa gatgactttg acgagttcaa aattaatttt
    47221 agaccagaac aaaaagatgc tattgagcag actaagactg tattcaaaac gaaagatgag
    47281 atgctttgga atgccaagat gcgttttggt aagacgcttt cttctcttca agtgattaaa
    47341 gagagccaat acaaaaaagt tcttatcatg actcatcgac ctgttgtaag tgatggttgg
    47401 tttacggatt tcaaaaaaat cttcacagat ggttcgtaca tctatggttc aaaacaagat
    47461 ggagaaaaga ttcaaaacct tgttgatacc gaaaaacctt tcatctattt tgcaagtatt
    47521 caggacttac gcggttctga gtgggcaggt ggaaaagcag gggataaaaa ctatgaattc
    47581 ttagcgattg actgggattt tattattatt gatgaagctc acgaaggaaa cgaaacggaa
    47641 ttagctaata acgtaaaggc taagttgcgg aaagagcata ccaaggtctt agagctttcg
    47701 ggtacaccat ttaacctttt cgataagtat gatgaggata atattttcac ttgggattat
    47761 actatggagc aagaagctaa ggagaaatgg tcagtagagc atcccaatga acctaaccca
    47821 tacgaaggtt taccgaaagt atctatgttc acctttgaaa taccaaataa atttgattat
    47881 ttcgatgaaa ataaggcatt taacttccga gaattcttta gggttaagga agatgatgaa
    47941 acaaagttgc ttcatgaagg tgacgtacgc aagtttcttg attatattac tacaaacgat
    48001 gaaaagacta attttccttt ctcaaaacca gagtttagag aaaacttacg gcatacttta
    48061 tggctgatgc cgggggttaa agaggcaaat gcttttgaaa gagtgctcga aacgcaccct
    48121 atattcaaag attacaatat tgcaaacgta gtcaagacag gtgacagtga gcatgctagt
    48181 gattcagatt tagaattggt gcgtaaagct attggtaatc cagctaagtc taagacgata
    48241 accttaacag ttcgcaagct tacaacagga gttaatgttc cagagtggac aggggtattt
    48301 ttcctttcaa acacagaaag tcctacatct tatctgcagg cagcgttccg tgcacagaca
    48361 ccttttaacc atgcagagct tggaattaag aaaaactgct acatctttga ttttgcacct
    48421 gaccgtgctt taaaaattat gagtggaagt gtcggactaa ctagcaagaa gggaaaggtt
    48481 aatagttcag agcaaaagaa aaaactagag cgcatgctaa atttcttacc tatccttggt
    48541 aaacagggga atgtgatgaa agaatttagc gttgaccgaa tgcttacggc actgaagaag
    48601 gcttatgcag aaaaagctgt acggactggc tttgaagaca gctctcttta caatgacaac
    48661 ctattaaatt tagaccaagc tgatcttacc aaatttgaag accttaaaaa aattgttggc
    48721 tcaagtaaac caaccaaagt gaaagacttc attgtttcag ataacggtat gagtgaagag
    48781 gagtatgaaa aagcttcgag ggcagaaaag aaaccaaaac gtcaacgtac caaagaggaa
    48841 caggaagcaa tagatagggc aaagaggctt cgagagcagc gaaataaaat gatttccatt
    48901 ttgaggggcg tttctattcg catacctatg atgatttatg ggatggacat tgatattaaa
    48961 gatgacgtta ctattgagat gtttcttaat ttggttgatg aggcttcatg gaatgagttc
    49021 atgccagagg ggttaagtaa agggcgattc agagagttta ttgagtacta tgatgcagac
    49081 gtttttgtgg aggcaggacg cataatccgt agacgtgcaa agtcttatga tgagttagat
    49141 gttattgaac gtactaggaa aataggtgaa ttatttagca cttttaaaaa tccagacaag
    49201 gaaaccgtat tgactccatg gcgagttgtt aatatgcacc ttggtaacac tatcggtgga
    49261 ctttccttct ttgatgaaga ataccaaaat tctacggaga acggtcaacc aatcgtgcga
    49321 tggaggaaaa ctgactgtac agatgatatt tttaaggaaa atagaaagat tcttgagata
    49381 aactcaaaaa cagggctata tcctttatat gcagcaacga gtatgtacta tgttcagctc
    49441 cttaaacaaa gcgatattaa tgctggaagg gtagattcag aggcaatatg ggaaaaagta
    49501 ttatcagagc aaatttttgt catttctaaa actccaatgg ctaagacgat aacgaagagg
    49561 actttgattg gttataacgc atggaaggta aatgttgaat actcaagtga attagttacg
    49621 agtatgaaac gttcaatcaa tgacggttta acgaatttga aaggagttta taaaaacgtg
    49681 aaatttgatg tgataattgg taacccacct tatcaggaga aagatggagg ttcaggagct
    49741 agtgctacac cgctatataa tctatttgta gaaacctctg ttaagttatc agaacgctat
    49801 gtttcgatga ttactccttc gaagtggttt gcaggtggta aagggttaga taagtaccgt
    49861 gactttatgc ttaacagctc tagcttgaag gtgatggtgg atatagtaaa tgggaaagaa
    49921 atattcccaa acaccagtat tggtggcggt gttagctatt tcttgtggga taagaaatat
    49981 aatggattga cacagtatac taatcaacat gatggtgtat caaatacaca agagcgaaaa
    50041 atgaacgagt ttccagtgtt tgtgcgatat aatgaagcca tttctgcaat tcacaaaatt
    50101 ggggaacacg ctagtgttgg tgactttgtt atatcacgta acccatttgg gcttccaacg
    50161 aaaattcgag gggaagaagt gcaggattcc agaaattcaa taaaagtaat ctctagtggt
    50221 ggggtaggct ttgttccact ggaagatatc actaaaggac aggatatggt tggaaagtat
    50281 aaagtcatta gtagctcact gactagcgaa catgcaggtg aaccgaacaa aaatggtatg
    50341 tttaaggtga tttctactaa cagagttatt aaacctaatg aagtagttac agattcatac
    50401 ctagtgctat tttctgctga taccgaaacc gaagcaatta attatctgaa gtatttacga
    50461 acacgttttt acagattttt attacttcaa gcagtgacat ctattcacct atccaaagat
    50521 aagttcatgt tcataccagt acaagatttt acctcaggtt cagacattaa ctggaatgta
    50581 ggtattgaag aagttgatat gcagttgttc aagaaatatt ctttcgatgc aagtgagata
    50641 ttgtttattg accaaacaat cgtcccgatt cttcacgagg aatattacta agagaacaag
    50701 ctaacaaatg aggcttagaa ccctgttttc ttgattaatt gccccacttt aaatcaagat
    50761 ttaactgtct attatcaaca ggtcaaggta aaataaagga tgttagcaag taaagagatt
    50821 gatttgagtc tacatcgaac aagggaataa tccaataaaa aatgatttag aatat
//
LOCUS       DAXLPI010000006        50398 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXLPI010000006 DAXLPI010000000
VERSION     DAXLPI010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.006"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..418
                     /locus_tag="ACF3T5_000283"
     CDS             <1..418
                     /locus_tag="ACF3T5_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFD3630926.1"
                     /translation="ICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANIFNHLKKML
                     NVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADIS
                     FAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            699..1919
                     /locus_tag="ACF3T5_000284"
     CDS             699..1919
                     /locus_tag="ACF3T5_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFD3630927.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1916..4636
                     /locus_tag="ACF3T5_000285"
     CDS             1916..4636
                     /locus_tag="ACF3T5_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3630928.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4620..5564
                     /locus_tag="ACF3T5_000286"
     CDS             4620..5564
                     /locus_tag="ACF3T5_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFD3630929.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5633..6643)
                     /locus_tag="ACF3T5_000287"
     CDS             complement(5633..6643)
                     /locus_tag="ACF3T5_000287"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFD3630930.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6823..7167)
                     /locus_tag="ACF3T5_000288"
     CDS             complement(6823..7167)
                     /locus_tag="ACF3T5_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFD3630931.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7160..7594)
                     /locus_tag="ACF3T5_000289"
     CDS             complement(7160..7594)
                     /locus_tag="ACF3T5_000289"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFD3630932.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7732..8469
                     /locus_tag="ACF3T5_000290"
     CDS             7732..8469
                     /locus_tag="ACF3T5_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3630933.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8462..9673
                     /locus_tag="ACF3T5_000291"
     CDS             8462..9673
                     /locus_tag="ACF3T5_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3630934.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9877..10659
                     /locus_tag="ACF3T5_000292"
     CDS             9877..10659
                     /locus_tag="ACF3T5_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFD3630935.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10729..11391
                     /gene="trmB"
                     /locus_tag="ACF3T5_000293"
     CDS             10729..11391
                     /gene="trmB"
                     /locus_tag="ACF3T5_000293"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFD3630936.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11489..13243)
                     /gene="ade"
                     /locus_tag="ACF3T5_000294"
     CDS             complement(11489..13243)
                     /gene="ade"
                     /locus_tag="ACF3T5_000294"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFD3630937.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13375..14304
                     /gene="comGA"
                     /locus_tag="ACF3T5_000295"
     CDS             13375..14304
                     /gene="comGA"
                     /locus_tag="ACF3T5_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFD3630938.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14261..15310
                     /gene="comGB"
                     /locus_tag="ACF3T5_000296"
     CDS             14261..15310
                     /gene="comGB"
                     /locus_tag="ACF3T5_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFD3630939.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15307..15585
                     /gene="comGC"
                     /locus_tag="ACF3T5_000297"
     CDS             15307..15585
                     /gene="comGC"
                     /locus_tag="ACF3T5_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFD3630940.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15560..16030
                     /gene="comGD"
                     /locus_tag="ACF3T5_000298"
     CDS             15560..16030
                     /gene="comGD"
                     /locus_tag="ACF3T5_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFD3630941.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16002..16349
                     /locus_tag="ACF3T5_000299"
     CDS             16002..16349
                     /locus_tag="ACF3T5_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630942.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            16312..16737
                     /gene="comGF"
                     /locus_tag="ACF3T5_000300"
     CDS             16312..16737
                     /gene="comGF"
                     /locus_tag="ACF3T5_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFD3630943.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            16709..17068
                     /gene="comGG"
                     /locus_tag="ACF3T5_000301"
     CDS             16709..17068
                     /gene="comGG"
                     /locus_tag="ACF3T5_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFD3630944.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17240..18247
                     /locus_tag="ACF3T5_000302"
     CDS             17240..18247
                     /locus_tag="ACF3T5_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3630945.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18272..19456
                     /locus_tag="ACF3T5_000303"
     CDS             18272..19456
                     /locus_tag="ACF3T5_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFD3630946.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19893..21476)
                     /gene="abc-f"
                     /locus_tag="ACF3T5_000304"
     CDS             complement(19893..21476)
                     /gene="abc-f"
                     /locus_tag="ACF3T5_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFD3630947.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(21581..21709)
                     /locus_tag="ACF3T5_000305"
     CDS             complement(21581..21709)
                     /locus_tag="ACF3T5_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630948.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(22054..22197)
                     /locus_tag="ACF3T5_000306"
     CDS             complement(22054..22197)
                     /locus_tag="ACF3T5_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFD3630949.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22419..22898)
                     /locus_tag="ACF3T5_000307"
     CDS             complement(22419..22898)
                     /locus_tag="ACF3T5_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFD3630950.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(22971..23255)
                     /locus_tag="ACF3T5_000308"
     CDS             complement(22971..23255)
                     /locus_tag="ACF3T5_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630951.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23333..23527)
                     /gene="ssrS"
                     /locus_tag="ACF3T5_000309"
     ncRNA           complement(23333..23527)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACF3T5_000309"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23599..24885)
                     /locus_tag="ACF3T5_000310"
     CDS             complement(23599..24885)
                     /locus_tag="ACF3T5_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFD3630952.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25023..25694
                     /locus_tag="ACF3T5_000311"
     CDS             25023..25694
                     /locus_tag="ACF3T5_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFD3630953.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25660..26148
                     /locus_tag="ACF3T5_000312"
     CDS             25660..26148
                     /locus_tag="ACF3T5_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFD3630954.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26161..27108
                     /gene="prmA"
                     /locus_tag="ACF3T5_000313"
     CDS             26161..27108
                     /gene="prmA"
                     /locus_tag="ACF3T5_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFD3630955.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27109..27873
                     /locus_tag="ACF3T5_000314"
     CDS             27109..27873
                     /locus_tag="ACF3T5_000314"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFD3630956.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28106..30319
                     /locus_tag="ACF3T5_000315"
     CDS             28106..30319
                     /locus_tag="ACF3T5_000315"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFD3630957.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30330..30776
                     /gene="dtd"
                     /locus_tag="ACF3T5_000316"
     CDS             30330..30776
                     /gene="dtd"
                     /locus_tag="ACF3T5_000316"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFD3630958.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30947..32206
                     /locus_tag="ACF3T5_000317"
     CDS             30947..32206
                     /locus_tag="ACF3T5_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFD3630959.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32295..33242)
                     /locus_tag="ACF3T5_000318"
     CDS             complement(32295..33242)
                     /locus_tag="ACF3T5_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFD3630960.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33636..34928
                     /gene="hisS"
                     /locus_tag="ACF3T5_000319"
     CDS             33636..34928
                     /gene="hisS"
                     /locus_tag="ACF3T5_000319"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFD3630961.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34943..36712
                     /gene="aspS"
                     /locus_tag="ACF3T5_000320"
     CDS             34943..36712
                     /gene="aspS"
                     /locus_tag="ACF3T5_000320"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFD3630962.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36821..37696
                     /locus_tag="ACF3T5_000321"
     CDS             36821..37696
                     /locus_tag="ACF3T5_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFD3630963.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            37962..39845
                     /locus_tag="ACF3T5_000322"
     CDS             37962..39845
                     /locus_tag="ACF3T5_000322"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFD3630964.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39920..40204)
                     /locus_tag="ACF3T5_000323"
     CDS             complement(39920..40204)
                     /locus_tag="ACF3T5_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFD3630965.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40194..40928)
                     /locus_tag="ACF3T5_000324"
     CDS             complement(40194..40928)
                     /locus_tag="ACF3T5_000324"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFD3630966.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41057..41680
                     /locus_tag="ACF3T5_000325"
     CDS             41057..41680
                     /locus_tag="ACF3T5_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFD3630967.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41872..42993
                     /locus_tag="ACF3T5_000326"
     CDS             41872..42993
                     /locus_tag="ACF3T5_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFD3630968.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43000..46128
                     /locus_tag="ACF3T5_000327"
     CDS             43000..46128
                     /locus_tag="ACF3T5_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3630969.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46147..49341
                     /locus_tag="ACF3T5_000328"
     CDS             46147..49341
                     /locus_tag="ACF3T5_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFD3630970.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49487..50194)
                     /locus_tag="ACF3T5_000329"
     CDS             complement(49487..50194)
                     /locus_tag="ACF3T5_000329"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3630971.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16456 a         8857 c        10499 g        14586 t
ORIGIN      
        1 tatttgttca atcccttata ataatattga ggaaattata gaaaaggatg ctatcagcat
       61 ttacaaattt tttattttta ttcctaatcg tcaattgaaa gctaatatat ttaaccattt
      121 gaaaaagatg ctaaatgtgt ctgttactga atcggcacct gtaaatattg aaatcgtacc
      181 tcaaaatgta tctaaaggtg tggtgtttga tcgtttggaa tcaatctaca atttaaagaa
      241 acctttgaga atcgcgatcg gagatagttt aaatgactta tctatgtttg aaagtgcaga
      301 tattagtttt gcaatgggaa atagtcatca agaaatcatg aaaaaagctg attatgtcac
      361 tgtttcaaat gataaagacg gagtatctca agcatttgaa agaattctta ccatataaac
      421 taattaaggt agtatagaac atagaattct aaatacggaa aaatggagca taaattccag
      481 tacaaatagt acttcttcag tgtcaaacga agcgaatata acatagatat ttaggattgt
      541 gacaaaaatc atgtcacaat ctttttttcg aataaactta ttttttgaag tcggccattt
      601 atcgtaattt ctttgcataa tacgaacaaa ttttcggtta ttgatgaaaa aaaacattga
      661 aaatggtatg ataaaagaaa aaaaggagga aatttttaat ggtgaaattt atccatgcgg
      721 cagatttaca tatggatcgg tcatttgaag gactgacaac tcttgataaa actgtacagg
      781 aaaaactatt gaagacgaat ctgaccgttt tatcaaatat cattgaacag gcaataataa
      841 ataacgttga ttttgtttta cttgcaggag ataattttca tcaaaatcgt ccttcattaa
      901 aaatacagaa acatttttca gaacagatga aacggttaga aaagaaccat atacctgtat
      961 ttataatatt cggaaatcat gatttctatc aaaaagagcg gtattggttc tcctttccta
     1021 aaaatgtcca tttatttaca tccgaaacgg tagagacaaa aaaaataacg ataaaaagtg
     1081 gagaaactgt ctctctcagt ggatttagtt atcgtcaacc gtggattcaa aaagacaaag
     1141 tgatggaatt tccttctaga gaactcacag attaccatat tgggttgtac cacggggaac
     1201 ctggcgtctc tcaaaaagga aactatgctc ctttccaacc ctcaaagatg caagaaaagg
     1261 gatacgatta ttgggcttta gggcatatcc atgtgccaac ggtcttaaat gaaaagcaaa
     1321 caattgttta tccaggtgcg cctcaaggtc atactaaaaa ggaacaagct tctacatcta
     1381 ttctactagt tgaattaagt aaaggaagtt gccaaataca tccgattaaa gtagcagagg
     1441 tctattggaa gacaaaggaa atatcattga ggaaagcaag aacgacaaaa gaggtcattg
     1501 ttcacattcg tcagcagctt tcaaaagtag aggatggatt tagtttaatt gagataaaat
     1561 taaaagatta tgaccatctc aatacagatg ttttggaacg gatacaatca ggagagctat
     1621 tagactattt gttagaggaa ttctcagata ggataaatga tttctttatt tggcgtattt
     1681 ctctcataga gaatactttt tatacgaaga ccccacttgc tgcttctgct aaattgatgg
     1741 aacagttatt tcaatactat cagacacctc aagatttcca acaaatttta aatgaagtat
     1801 atagtcatca agaagcggct agaatattga gcgaactgcc ggaatatcaa gaagaaacac
     1861 ttgaaaaagc caagcagctg cttaatcaag attttttatt tgaggaggat caggaatgaa
     1921 gattttaagg atcgagattg ctgcttttgg aaaatggcgt caaaaaagtt ttgactttta
     1981 ttcaggcaat cagctgatct atgggggaaa cgaagcagga aaatcaacga tttatcaatt
     2041 tattcaagca atattatttg gtttcccttc aaaaggccgt aaaaaaaagg attatacacc
     2101 aaaagacggc tcagcctatg gcggcaaaat atggcttaaa catcctgtat atggtgaatt
     2161 tgctgtcgag cgctacaagc agcaaaatcg cggaaaatcg aaggtgtggc tgggggatca
     2221 agtgggaagt gatgagttac tagaaaaact gatcgcacct ttgacgaaag agttattcca
     2281 gcaggtattt actttccagc aagagcagtt gatggaacta gatcatttga gagaaaaaga
     2341 gctacatgat gcgctgattt ctttaggcat cacgggcagt tctcaagtat tccaaaagcg
     2401 gcaagaatat tatcagaaag cacaacaatt attcaagaaa aaaggccaaa aattactaat
     2461 caatcaaaaa ttaaatgaat ggaaagagct gcaagaaaaa attcatcaaa aacaagaaca
     2521 ggaagcttat tttggcaagt tgatccaaca agaacaggaa tacgagtcgc aacagcagca
     2581 gttattggca caattaaaag aggcgaacaa acaattttca ttagccagac agcaagaact
     2641 taatttttct ttatatgaag aatggcaatc tctcaaaaaa aacagcacta caggtcttcc
     2701 tgatgagcaa cttcttttag atctggagtc tttctccaaa cggtatcagc aaacaaatga
     2761 gcgtttggaa gaattagaag caacactgga aaagcatagc gggatggatc agcagtcagc
     2821 cagatattat ttttatttag agcaggagca ggaactaaaa gatctccagc aacttcaatt
     2881 tacagcggaa caattaaacg aagaatggca aagattacaa attgagacta gagaaacaaa
     2941 ttcagaattg aagattttgg aacaaaggtg gcattggcag aaaaatccgt taccggaaga
     3001 atttttatat gaagaagaat ggaagcatct tctggctgaa gaaagaagga ttgctgatct
     3061 agaaagccag atttctgtgc gtctccaaat agctaaagaa cagcaagaga tgattgaagc
     3121 agaagtgaat gacttggaag agacctatcc agcattatta caaaatcagt cagagatagc
     3181 accagtctct tcatctaaac cgtatatgct gatggctggc ggagcaataa tcattgcagg
     3241 tatatttgta ccaatgccct tgaaagtgat ccttttacta attggtgcag gcatgattgg
     3301 ctatttcttc tacaaaaggc aaccaaaaat taaaactaaa aatgatttct ctgagataaa
     3361 aggaatgtgg caagaaaaac taggacaact tgatctagcc ttagctcaat tagctgaagc
     3421 agaagaaaaa caaaagagaa ctggagaaga aaaacagatt cttcagcaag aagtatatcg
     3481 ttttgcggat catcggcact taggaaagat ggatacgctc aaactcatga aagaatatgg
     3541 acaggaagtc atagattatc agaaattata tggcgtattt gagcggaaac ataaaagaga
     3601 acaggaaata caagaaaaac tacgacaaat cgaccaaaaa tttgagtttg ttaaagaatg
     3661 gttaccgata caagataaga caatttcaga aaaaatggta ttgctttccc gttttataca
     3721 agagatggaa cagatgaaat ttgccagaag ctatcagcaa aatactttat tgaagcaaga
     3781 aatcaatcaa ttgaaaaaga aacaaaaagt atggatacaa gaatacagcg aacaattgac
     3841 tcaagcaggc ttatcttatc cttcggaagt acctgtcttt ttacaaaaag ccaaacaagc
     3901 cgaacagcag atgaaaagat acaatgaatt atcggaaatg cttactccac tatttcctga
     3961 agagctgaca aaagagaaat tatctgtaag actgcgccaa ctgaaagaaa agcaatccca
     4021 actccagcaa gaagaaaaag aagcaagtga aaatcggcaa cgactccaac ttcaagtaga
     4081 agagttgcaa agagacggca cattggacga actttatcaa caagctagcc ggcaaaaagc
     4141 aaaattacag gaattactgg aggaatgggg agcacttgaa atcgccggga cattcttagg
     4201 tgatctttca acagagatga gtgaaagaca attaccccag ttgttaagag cagcaggtga
     4261 ttatttaggt attttaacaa aaggaaatta tcagaagata ttactgaaaa atgatacact
     4321 gactttaaca gacggatata cagaatttcc catctatgag ttatctacag gtacgaaaga
     4381 tcagctgatt atggcgatga gatttgctta cctctcatta gaagctaacc gttcgatatg
     4441 cccgatcatc attgatgatg gctggcttca ttatgatcat cagcgaaagt atcaattagc
     4501 tgaactttta gctaaatttg gagaaaagta tcaaataatt tgtttttctt ctgataaaga
     4561 aatggtaagc tattaccatg agatgaacca aatcgtaaaa caattagaag gagcaaataa
     4621 tgaaaaaaat tcgtgattta gtaatcgatg agcagtttga acaatttgtc ctgatcaaaa
     4681 gtgcagatgt aagaatcgct aaaaacggaa aaaaatttat tgcttttact ttccaagata
     4741 cttcaggtac tatcgatggt aaatactggg atgcttcgga ggaggaaatc aataaattta
     4801 cagccggcac agtcgttttt ctaaacggta aaagagaggt ttatcaagga aaccctcaag
     4861 taaagattct tcatatgcgc ttaacaaggg tagatgagcc aggagagcct agtttataca
     4921 tggaaagagc ccctttgaag aaagaagaaa tggaagaaga aatcaatcaa ttgttgtttg
     4981 agatcaccaa tgcgcattgg aatcgtattg ttcgctatct acttaataaa tatcaaaaac
     5041 aattttttga gtttccggca gcaaaaagaa accatcatgc atttgccagt ggattagctt
     5101 atcatacttt gacaatgctt cacttaggga aatcaatcgc cgatgaatat cctgagctga
     5161 atcgagcatt gttgtatgct ggtatcattt tgcatgattt aggaaaggtg attgaacttt
     5221 ccggtcctat gtcgacagaa tatacgcttg caggaaattt gatcggtcat ctggtattga
     5281 ttgacgaaga aataactaaa gcctgtcttg cgttgaaaat cagcgaagct gacgaagatg
     5341 ttcttgtatt acgtcatatg gttttagcac accatggctt attagaatat ggctcacctg
     5401 tacgtccaag aataatggaa gcagaaatat tacatcaaat cgacaatatc gatgcttcga
     5461 tgcagatgat gttgacttcg attcgtcaaa cagaaccagg aaaatacacc gatcggattt
     5521 tcggtatgga caaccgaagt ttctatgtac caaaagattt atagcaaaaa tttatttttt
     5581 tcattcaaaa caaaaaaagc gagacataag tcttcgcttt ttttgttttc ttttattttg
     5641 atgaatcagt tgtttcttca gttgaagatt cttttgttga atcagttgat tttgtgtcag
     5701 aactctttgt tgaagctgta gtttcacttg agtctttcgt acttgatgaa gttgttgtga
     5761 atgcactcaa cacattttca aaggcatcat ctttgatttt tacatttgca tcttttaatt
     5821 cttcaccgat tactttagtt gtgaatgcat tgtcacttag tttagtttca gtagcgatat
     5881 cttttagttg atctttgtat ttatccatgt cattgccttt attttggttt ttcaccattt
     5941 tgacaacgta gtattctgtc gcataagaag taggatttgt tgtagtaata acatcggaga
     6001 tctcaccgtc tttcaatttg aaagctgctt cttttacttc tgcaggaata gttgttgtcg
     6061 ttgagtcgaa tttaacttta ccgccatctt cttttgtctc tgtatcagtt gatttgtctt
     6121 tggctaattt agagaaatca tctccatcat ccgctgattt tttcacatct tttgcttcat
     6181 cttcacttga taatttgatg atttgtgctt ctacttctgg atggaaagat ttccaagcag
     6241 ttttcaaatc gtcatctgta atatctacgt gagctttcaa gccagcttca aaagctaaat
     6301 tgttacgaat atattcttta taagtatctt tcgtatatcc agcagcttct aattgtgatt
     6361 caaaagtatc acctaatgat ttcgcttgtt tgtcgtattc tgcatccact tgcttatcgg
     6421 ttactttatc gccatatttg ttattgaata ctttataaat gatcatccgt tgtactaagg
     6481 attggttcga tgattcaagt tttgcttcat catagaaatc agatacagtg atcgttccgc
     6541 ctttcatcgt tgcgatatct ttattagtat cgcctgaaca agctgctaga gttaaaacag
     6601 ccaacgcact agtagctgcc aaaataattg attttttttt cattaaaatt tgcactcctg
     6661 taattagtta ttatttttat atattaacta taccatatcc catttccttt aaaaacgttg
     6721 tcgtggtagt ttcaaacaaa attcaaaatt tcatcacatt cctaggaaat acgcaaactt
     6781 ttctcactta tatagttaaa tctgttgaga aaataatcat tattatattt gttcaggata
     6841 ctctggcaga tcagctttta gtttttctac ttgttcattg atttgtgtga tacgtggttc
     6901 ggcttggaat ttaaaatctt cgatatcttt tttgattgct tcttggaaag caggaatcac
     6961 agattcagct gtttcttttg tttcgatcaa tgcgctttta aaatgattca ggctgttatt
     7021 taaatcattt gtcagagttg ttgcttcttc caattcttcg atcagtttct ttcttgtttc
     7081 attcccgctc ctgggcgcca ataacagacc tccgacagtg ccagcagcag ctccaaataa
     7141 cagtcctttg aaaaagtttt taatcatcgc aggacacctg ctttacgatt gtttcttgaa
     7201 tagcttttag ttcttccgat ccataactat cttgatgatt gacaaagtga atagaaaaat
     7261 catcctcttt gctgtagcga gggactaaat ggatatgtga atgaaaaaca gattgatagg
     7321 ctagttcacg gttgttattc aagatattaa gacctttcat ttctggaaaa gctttttcca
     7381 atgcttgagc aatcttaggc acacgcgaaa aaacctctcc tgcaagttcg ggatcatatt
     7441 caaaaatatc tgctacatga cgtttaggca caactaatgt gtgtcctttc gttgcttgag
     7501 agatgtctaa aaaagcatag accttgtcgt cttcatatat tttatagctt gggatttctt
     7561 gattgatgat tttgcaaaaa atacagtttt ccatataaaa catcctttct ataaagaata
     7621 aatacttcag cttaacttta ccataagtta aagctattgt aaggaaaaaa acaaaatacc
     7681 gtcattttct tcgtggtata ataaataaaa ataaatggcg gaggaaaaag aatgagttta
     7741 attatcgatc atgtcacagg ggggtacgga catctccctg tgttaaaaga tatcagtttc
     7801 gatgtacagc ctggagagat agtagggttg attggactga acggagcagg gaaaagtacg
     7861 accatcaagc acattatcgg tctgctggaa gcttcaaaag gaacgataga aatcaatcat
     7921 ctatccttaa agaaaaatac ggaagaatat cgcaaacaaa ttgggtatat tccagagact
     7981 ccttctttat atgaagaatt gacattgaga gaacatatcg aagtcactgc gatggcctac
     8041 gatataccta tggatatagc aatgaaaaga gcagaaaagt tgttggagac ttttcggtta
     8101 tctaatcgtc ttgactggtt cccagctaat ttttcaaagg gaatgaaaca gaaagtgatg
     8161 atcttatgcg cctttttgat cgagccttct ttatatatca tcgacgaacc atttttaggc
     8221 ttggatccac tagctatcaa tgcattgctt gaattgatgg tagaaatgaa aaaagaagga
     8281 gccggtattt tgatgtctac gcatatttta gctactgcag aaaaatattg tgatcgtttt
     8341 gtggtcttac ataacggaga gatacgagca gacggttctc ttgagatgtt gagaaaagaa
     8401 tttcagctgc cggattcttc tttggacgaa atctatattg ctttaacaaa ggagcaggca
     8461 catgagtagt ttttttcaaa aacgtttagc aagacatcaa aagaaaatga tgaaatatat
     8521 gcgttatgta ttgaacgatc attttgtttt agtttgtctt ttcttgattg gcggtgtggg
     8581 attatattat tctaattggt tgaaaaccct gcagccaccg tttgcaattg gagggctcat
     8641 aatctctgta ttttggatgc tctgtttatt cgtgggtaag atggctactt ttgcagaggc
     8701 agcagatatt gtttttttac ttcctaaaga aaaagaaatg caaacctatt tggaacgagc
     8761 ttttcgctat tcttgcattt ttccgttgat ctttttgata ttggcatgcg gattggccat
     8821 gccattagtg gtggttagta cggagcaatc cttttcgatg tttttcgtct atctgatcat
     8881 attatggagt ttgaaattca gtcatctgca aatcaagaga gcagaattgt ttcgactaga
     8941 tcaacaagtt attcgttatt ggaaattagg ctggtttttt tcttctctga tctttttgtt
     9001 tctatcattt tattggctaa tatgggcagg aatagcaggt gcattagtac aggcaagtct
     9061 atattactgg tttttgtgga agcaacaaga aaaaggattg gattgggaga aaatggtgca
     9121 tgtagaagaa cataggttgc atcagatcta tcaatttatc aatttattca cagatgttcc
     9181 tgaaattacg gcaaagatca aaagaagacg ctatttggat cctatattga ctcatattaa
     9241 aagagaatca aaaaacactt atctttactt gtttgcaaga agattggcaa gaggttctga
     9301 ttttagtggt ctttatttcc gattagtaat tataggtggg ttattgattg ccggagtagt
     9361 cgacttttat tttagtctga ttatcggttt cttattttta tatttgatcg gtttccaact
     9421 gttaccttta gcgaaccagt tccgctatat ggttctcgtt cagctttatc caatccataa
     9481 gaatcagaag aagcaggcga ttcagtatct actcacatgg gtattaatta tcgcgtcggt
     9541 tatttttggt ttgattgctg cagtcgtatt agatgggcag cagcgatggg ttccgctagc
     9601 ggcttatttg attgttacta tgctctttgt taaactatat gtaccaactc gtttgaagaa
     9661 gatgtcagac tgaaaacaaa gctgtgtagg gtaaaaatga gaatgaaaaa tagagcgttg
     9721 acagtaagaa tgctgttcgt tctatttttt ttgagaaaag ctggtttttt gaatggcaat
     9781 taaatgaaaa agaacgtata tgtgcttgac gtgtagtgtt caggcgacta gaataataag
     9841 tactgcaaaa caaagagtaa gaggacggga gaatgcatga tggagtttca gttagaccaa
     9901 gagtggcggt tacgtcccat caaaggcgac acaggaaaaa catacatagg catgcgtgat
     9961 gaggacaaag tctttattaa acgaaacacg actccgatgt tggctgctct ttcaagggaa
    10021 ggaattacac caaaacttgt ttggacaaaa cgaacgggga atggcgatac tttgacagca
    10081 caagagtggc tggaaggtcg cttgctgact cctaatgaga ttggtcaaag aaacgacgtg
    10141 attgatgttt tgtaccattt acatcattca aattctctta aaaccatgct gacccgtatc
    10201 ggtggtcgtg tcatgacgcc ggagaagatg ctgcaaaaat atgaaaaaga attaccggaa
    10261 gaactccaga aaaatcaatt tctgcaaatt gtccatcgtt ttcttcgtga gcagatcccg
    10321 tcaatagata aggaaaactt atcagtagta cacggagatg taaaccatcg taattggtta
    10381 gtatgtaaaa attatttata tctagtcgat tgggattcaa tcatgtttgc agaccctgcg
    10441 gtggatattg caacgatttt aggaaattat gtgccactgt ctagctggaa tcagtggctg
    10501 atcagttatg gcatacgccc gactgacgaa atgcttgaaa aatttcattg gtatgcggcc
    10561 atgaatttgc tccaagaaat caccagatat tgcaaacgtg aagatcatcg acggatgaac
    10621 gaagaaattt tacaattaaa acgaattttc agtggctgag gtttggccca aactctgcgg
    10681 agatttggcc agaccttttt aattagagga aaggaatgga gataaataat gcgtgtccga
    10741 aatcgtccgg gagcagcgga aatgttagcg gctcacccga attttgtaat tagtgatcca
    10801 acattgtgga agggaaaatg gaatgaattg tttgaaaacg atcatccaat ccacattgag
    10861 attggtatgg ggaaaggcca gttcatcact ggaatggcaa aagcccatcc tgaaatcaat
    10921 tatattggag tagaaatgca ggtcagtgtt gtttcgattg cactagacaa gttgatcgaa
    10981 caaccgcttc ctaacctgaa acttctacat gtggatggat cagcgttgac agagtatttc
    11041 gctgacagcg aagtagatca aatctatctg aatttttccg atccatggcc aaaaaaacgt
    11101 catgaaaaac ggcgattgac atataagact tttcttgctg tagatgaaca gattcttcgt
    11161 ccaaatggag aaatccattt caaaacagat aatcaaggat tgtttgagta ttctctggca
    11221 agtttttctc aatatggcat gatcctcaaa caggtatggt tagatttaca tcaaagtcaa
    11281 tttgaaggca atattatgac ggagtatgaa gaaaaatttt cttcaaaagg tcaacgtatt
    11341 tatcgagtag aagctcaatt tcaagataag accaaaaaga aaagtctgtg aaactaactt
    11401 tctgctttca taaaaacatt aatatccata aataaatctc agaaaatgaa cattacagat
    11461 gtaatgtatt ttctgggatt tatttgattt aaaatgtatt tatttcatcg gtttttccac
    11521 aaggtggaat caattggaag cgagaaaatg aaaataatcc tttgtcggtg atcttcaatt
    11581 cgggtatgac agataatgtt aagaaagaaa gtgtcaaaaa aggatcaaag gttttagctg
    11641 ctcctagttg atgtgcttta gttattaatt cattcaactg ttcatagacg atcgaaaaag
    11701 gttcctgtgt catgattccg cctataggaa gagaaacaca agctatttcc tgctgtttgt
    11761 tgacaacggt catcccgcca cctttttgaa tcaaaagatt agcagcatac agcatatcct
    11821 cgtcgtttgt cccaacgaca actagattat gcgagtcatg ggcaatcgtt gtcgctatcg
    11881 ctccttgttt cagctgaaat cctttcacta tgcctaatcc aatgtttccc gtttgatggt
    11941 gacgctcgat caccgcaatc tttaattggt ctttttcgat tgaaggaatg aatcgttcgc
    12001 cttgtcggtc cactttttct accaaatctt tagtcactag actattcggg ataatttcga
    12061 tcacatgggc ttgtgaagaa gaaagaagca gctctaacga atgtggattt aatggttgag
    12121 cgtttagttt tggcagacaa cacgtatatg aatttgaatg atcagcaaat aaatggtctt
    12181 ggagttgccc agattgaaca actaatgttc ccttatagaa aacttcatga attggcagct
    12241 tggtaagatc gtctgttaag aagaaatcag cttgataccc aggggcaatc gcacctagtg
    12301 ttttcaaatc aaaacattct gctgcattca atgttgccat ctgaatggct tgtaatggat
    12361 cgattccaag tgcaatagct tgacggacga tatgatcgat tgatccttct tttactaaat
    12421 cattgatcag tttatcatcg gtgacaaaga agcatcttct tgaattttca ggtgtgatcg
    12481 ccggtagtag cgatgccaga tcttttgcaa cggtcccttc tcgaaccatt aaatacatac
    12541 caagatccaa acgttcttta gcttcttttg cagttgtagc ttcatgatcc gtccgaatac
    12601 ctgctgcagg ataaacattt aactcttcca tgccgatacc agctgcatgc ccgtcaattt
    12661 ttttcttatg ctggtgcatc aattggatct tttctaggat gtctgtttca ttatttgcta
    12721 ctgcttgata gttcatgact tcagctaagc ctaaaacttc gggtttttgt aaaaaagggg
    12781 ctaatttttt tgcatttaaa tttgcaccat tggtctcaaa aggggttaca ggtacacaag
    12841 aaggtagcat aaaaaagata ttcatgagtg tttgtcttgc atcttctatc ataaactcaa
    12901 gacctgtact cccagcaatg tttcctattt catggggatc tgtcactaca gtagtaactc
    12961 catgaagcaa ggatactttt gaaaattcgc ttggtgataa aagtgagctt tcgatatgca
    13021 catggccatc gatgaatcct ggcactagat attgcccatt agcattatgt acgctttttc
    13081 cttcgtattc accgattcct acgatcattc cttcgcagat tgctacatct ttttccatga
    13141 tttctttcgt aaagacgttg acgatcttag catgcttgat cactaggtca gcaggctttt
    13201 ttttacttgc tacttcgata tatttcctta atgttgtttc caatggagtg cctcccaaga
    13261 taaattttta taagtataac ataaattcgg cttttgccaa tatttttttg taaattcatt
    13321 ttatttgaaa caaaaagatc aaaaagacgt atgttttttg tggaggggag aataatggaa
    13381 ataaaacgat tatcggatca aatgataagt gaaagttttg ccaaacatgc ccaagacgta
    13441 tatatattgc cagcttcaga agtattttac atctattttc gctatggaaa aaaacgaaca
    13501 ttctggaaga aagtctcttc ggcagatgga cagcaactga tctcaagatt taagtatttg
    13561 ggacagatgg atatcggaga aaaaagaaaa gctcagttag gtgcatttac ttattcttta
    13621 gaaaaacagg aagttcgtct gcggttgtct tctgtaggag attaccttca acgagagagt
    13681 ttagtgattc ggctattata cggtactttt gaaacacccc atcgttgctt tagttctcga
    13741 gattttgcaa ctattttaca gaaaaccgtc agacgtggat tatacctttt cagcggacca
    13801 gtcggatcag gcaagacttc tctaatgtat cggttggctt tggaagaaaa attgcaagtg
    13861 attactatcg aagatcctgt agagatagaa gaaccgtttt ttctgcagct acagatcaat
    13921 ccgaaaatag cacagactta tgatacactt ttgaaactcg cattacggca tcgtccagac
    13981 ttgctaatca ttggcgagat aagagatgaa tcaactgcac atgctgcggt aagagcagca
    14041 ctaacagggc accgggtttt tgcaaccgta catgcacgca atcttgaagg tacattggtc
    14101 agaatccatg aactgacaaa gaaaaaagaa gagctatctg aatgtttagc tggtgtcatt
    14161 tatcaagaac ttttggaaga tgccaatcac catccttctg ttttatggag ttatcaattt
    14221 atggaggaag aatcagtaaa aaaatcatgg gaggaaagtt atgaagaagc tcaggaaaat
    14281 atttggcagt cagaactatt ctaaaggatt aacaagaagc cagcaaaacc agtttgcaca
    14341 aaccatggga gaactgcttt tatctggttt tagtctgcaa gaatcccttt atttattact
    14401 cacgatccgt tcgtttccac ctgttatttt ggcaaatatc caacatcagc taaaggaagg
    14461 gcaagcgttt agtgaggtac tttcgcaaat aggatatgga gcagagcaac tggtacagat
    14521 cgagttagct gaacgtcatg ggaatctgcc gacaactttg ttggaaattg ccaaacagtt
    14581 tcggcttgtt gaaggatttc gcaatgattt gaaaaagctg cttgcatatc cgatgctatt
    14641 gctcaccttt ttgctaggta tcttgtttag tatgcggttg ttcgttttgc ctcagcttat
    14701 tggttcagga atggtcatgc aagagcattg gggtattcag ctgatacaag tctttcattg
    14761 gtatgttcta ctattcatcc tagcggttac tctattggct ttactagtaa ggtatcgtct
    14821 gatgaagttg tctcctgttg atcagagtga atggataagc agacaattat tgattggacc
    14881 gatatattcc ttgtatcaat cggcttattt gtcactagag attggaaagc tttttcatga
    14941 aggtttcgaa ttaaggcaaa tcatagtctg cttaaaggaa acgaaacaag aaagtctgat
    15001 tcagcatctt gcctctaagt tgattcaagg attggaggca ggtatgtcat tggctgagca
    15061 gttccagaat tatcgttttc tatcagaaga attcagtaaa attgttttac agggagaggc
    15121 aaagggaagt ttaggtaaag aactgatttt ttacagcgaa tggacaagaa aacagctttt
    15181 caaacgtctt catcactgga tgcattttat tcagccaatc atttttttgg gcgtagcggc
    15241 tttgatttta ttagtttatg ccgctgtttt attacctgtt tacggaagca tagaggaggt
    15301 tttgccatga aaaagagaaa aatatcttat tatagcggtt ttacattgat cgaaatgcta
    15361 atcgtcttac tgatcatcag tgtattagtg ttgttgttcg tccctaattt gtcgcgttat
    15421 aggaatcatg tagaccaaga aagtcgtgaa gcgatcatcc agttggtaga cacacaaaaa
    15481 gagctctatg cgcttcaaaa taatggaaga gttcctactg tggaggaatt gttgaatgaa
    15541 ggctatatca aacgagaaca tgcagaaatc tatcagcggc cttgaaggta aggttttagc
    15601 atcaggatat acgttgtttg aatcactctt actgatttta ttacttacaa cgatcctttc
    15661 ttttccagtt ctcgcatttt ctgcatggaa gaatgaattg gcagtctatc aatttttcgg
    15721 ccaatttgaa aaacgaatct attctacgca aaaaatcgct attgtgaatc agatccaaac
    15781 aggcttttat tggaatagtg aagagaatca aatcattttt catgttccga atccagataa
    15841 aataaattgg gaagtattag atattcctga tgagattacc ttgaaacgcc atgcttctat
    15901 tacttttgct gctagaaccg ggaatgaaag ttcactaaaa gcttatcaat tttattggga
    15961 agaaaaagag cggacgatta cgtatcaatt ccagataggg agtggtcgct atacaaaaag
    16021 aattgaataa aacaggattt gttttgttgg agtcccttac agcgcttatg atcgcttctg
    16081 tctgtgtcta cttagtgagc tttggccagc tgtttttgct tcaggcagaa aagaaaagtg
    16141 gggaggaact gctatttatt cgaatgattt atgaagatat caagacgcag cggatgtatc
    16201 acaaaagacc agagcgaagg gaagaaatga tcccatacga tatccaaatt gtcgataata
    16261 atggagtaat cgaaaaagtg tttgtcaata tggagaaaga agcgatcagt attgaaaaaa
    16321 caagtaattc cagcatttac gctgattgaa tgtctaattg gactgatgat attgagcagt
    16381 ctattgctca cattcaatct tttgattcag caaaccatcc aagtaagtga atttttgcag
    16441 cgtaaagatc aaaaagagtg gctaatattt ttagcacaat tagaagaaga actcaagagc
    16501 agtcacaatg tctctgtgaa aaatcagcaa ctgcactaca cgatcgagga taaacagtat
    16561 atcatcgaaa gatatcaggc aatgatacgt aaaagaagaa cttccggcgg acatcaacct
    16621 atgttaacaa gtatcactga gcttcaatta ttggaaaaag aacacacaat ttctttttat
    16681 gtacactttg aaaatggaga agatggatat gccatatgga caaaaaacga tcagtagaaa
    16741 atgttataag ggctctattt tattttctgt cttgctttta tttcttttat gtacgttttt
    16801 attgatggca gcattagaaa gctaccgttt gtcaatggat cttaatatac gaatcaaaaa
    16861 ttattatata gcaaaaataa tgaagcagat ggctttatcg gaacagtcta cgcttgccga
    16921 aagttcggaa ggagtttttt actacacaac tggatctgta acatatcagt gggtacaaca
    16981 gtccctattc ttagaggtgg aagtctcacc gtttatcttt cggttcacag aagaagtaaa
    17041 aaatgataca gatactagta cagaatgatg gaatgttcgt gaaaacgccc tcttttctgt
    17101 tataacactg tttttaataa aaaaatgttt gccgtttcac aaataaaaaa cagtgaaaac
    17161 gattgaaatt ttgtcaattg ttcgatagaa ttaaacaggt gaaaaaattg ttataatata
    17221 tctagaaatg aggtgcacca tgttaccaga aaaaatggaa caagggttta atcaaaacct
    17281 ggaagctatt cagcttttac agaatgcatt gggaacttcc tttttagaag cctatgtcga
    17341 aaatgcagaa aatttaatag atgattatca agtacgtgtg gtggatggag tgccgacaaa
    17401 agagacaacg caacggatca cagctcttta tgaagagttg aagaaacttt ctttcgagcc
    17461 agaagaatgg cgcagactgt ctcaattact tttattgaag ggcagccaga cagaacattt
    17521 gcaggcgaac catcaactca ctcctgatag tatcggtttt ttatttgttt ttcttattga
    17581 acaactatac acaaataaaa aagcaccggt taagattctg gatattgcgg caggaatggg
    17641 caacttgtta ttaacagttc ttttaaacct atcgaatgcg gggtaccaaa cagaaggaat
    17701 tggtgtggat attgatgata cattgttggc tgtcgcagct tctacaagtg atttgacaca
    17761 agcaaatgtt caatactttc atcaagacgg gttgcaggaa ttattaatag atcctgtcga
    17821 ttttgcaatc agcgatttgc caatcggtta ttatccgaat gacgaaaaag caaaagagtt
    17881 tttaacaagt accgaagaag gccatagtta tgcacatcat ttattgctag agcaatcaat
    17941 gaaatatgtg aagccggatg gttttggttt gtttttgatg ccgagtggat ttttagaaac
    18001 ggatcaaagt gaagaaatca aaaaatggtt taaagaagaa ggttatcttc aaggaatgat
    18061 tcaattgcca gatgaattgt tccgtaataa acaatcgcaa aaaagtattt tgatcttaca
    18121 gaaaaaagga ccacaagcac aacaagtaaa agaagtcttg cttgtgaaac ttgcttcatt
    18181 aaaagaacca gagaaggtga cggaattttt caacgagttt aaaaactgga aatcatctgc
    18241 tctgtaatca ttcaacgaga aggagagtca tatgtctaaa acaattgcaa tcaatgctgg
    18301 gagttcaagt ttgaaatggc aattatatca aatgccagaa gaaaccgtag ttgcgaaagg
    18361 gatcgtcgaa cgtatcggtt tacaagattc tatttttacc atcaagtacg gcgataatca
    18421 aaaattcgaa caaatcatgg atatcgaaaa ccacgaagta gcagtaaaaa tgctgttaga
    18481 tcagttgatt gaactgcaaa ttttaagctc ttatgatgaa atcaccggag tagggcaccg
    18541 tgttgttcac ggaggagaaa cttatgggga ttcagtagtg atcgatgaga aagtaatgga
    18601 ccgcattgct gaattagcag aatttgctcc tttgcataat cctgcaaact taatggggat
    18661 caaagcgttt aaaaagattt tacctgacat tttgagtgtg gctgtattcg atacatcttt
    18721 ccactcaaca atgccaaagc aaaattattt atatagctta ccggtgtctt attatgaaga
    18781 tttcaaagta agaaaatatg gcttccatgg cacaagtcat cgttatgtgt cggaacgtgc
    18841 ggcaaaatta ttgaacaaac cgatcgaaga actaaaaatc atcacttgcc acttaggaaa
    18901 tggtgtatcg atcacagcag tcgatggagg taaatccatg gatacttcta tgggattcac
    18961 accattagct ggggtgacaa tgggtacacg ttcaggtgat atcgatccag ctgttctccc
    19021 atatttaatg gaaaaattag agattgatat tgatgaaatg atcaatgttt tgaacaagaa
    19081 atctggtctg ctaggcctaa caggattgtc tagtgatatg cgtgatcttg aaaagaacta
    19141 tgaaaaagaa catattcgtt tagcttatga cattttcgtg gaccgcatcc gaaaatacat
    19201 cggcggttat gtgacaacga tgaatggtgt tgatgcaatt gtctttactg ctggtatcgg
    19261 cgagaatgac ggacatgtac gtagtgaaat catcaaaggt atgtcttggt ttggttgtga
    19321 aatcgatcca gcgttgaatg aattgcgtgg tgaagaagta gatatctcta caaaagaatc
    19381 aaaagtaaga gtattagtga tcccaacaga tgaggaacta atgattgccc gtgatgtgga
    19441 gcgtctgcgc ggctaagcca aagcattcgt taggataaaa gatgaaacgc atgcaagagg
    19501 gaattcttgc atgtgttttt tttgcggaga agaccgcaaa atggtaattt aacttcaatt
    19561 tcccttcttt tttacaggat cttcttaggg aattttgctt ggaaaagtgg tcaaaaggga
    19621 aaagactggc attttattta ttgattttca ggaaaatccc aatattttat tttcattcat
    19681 ctttataagg gacattacat atgtaaaaag aaaaaacatt tttagatcag cctcgtgggc
    19741 tgtcgacttt attcttcgct ttatgtttag ctagaaaacc gatccgaaac attcattgat
    19801 cgaataataa aaacttgatt ttccacctgt actttgcctg aatcgtgaaa gcacaggtat
    19861 gaaaatcaag ttttttgttg cgaaaaaacg tcctagtcaa tcagttggcc gagcggctgg
    19921 cggctgatct gctcctgatc aataacgagt gcttcttgga agtgttgctg caaaaagtag
    19981 cggtcatggc tgactgctag gagcgttcct tgatagtctt gcagcatttc ttcaatttct
    20041 tctcgagcat agctgtcgag gtgattggtt ggttcatcca aaatcaggac gttgattttt
    20101 tctaaaaaga gtttcagcaa cgccaaacgg attttttcac caccggataa atctttgatc
    20161 cgtttaccca catcgctttg ataaaagcca cttttggcta attgccgtcg agcgacttct
    20221 tcgtttccag tcaatgtgat ggcgtaagcc agcaaacggc tatcgggatc ggcaaactgc
    20281 agctgctgcg gcaaataacc gatttgcaag ctactaccta atttgatggt gccaccgtaa
    20341 ggcttttcct caccgagtaa taatttgagt aaggttgatt ttcctgcacc attgtcgccg
    20401 atgatggcta aatgatctcc acgatagact gcaaaactgc tgtctttgaa tagtacttga
    20461 tcagcgtact gctggtgcaa gttttgaatg atgaaaactt ctttgccaga ttttccacca
    20521 ttagacaagc tttgcagccg atttttcggg ggaggtggcg gtttgacgag tgtcagtttc
    20581 gccaggcggc gttccagttc tttggctttt ttgaagaagg actcattgtc tccttcatgt
    20641 gcccactgac gaaaacgacg aatcatgact ttcaaccgct ggacttcttt ttgctgcagt
    20701 tcgtaatttt ttgtcagctc agccaagcga gcttctttca agactcgaaa acggctgtag
    20761 tttccgggat actcaatcaa tgcaccttct tccagttccc atgtttttgt ggtgacttga
    20821 tcgagaaatt gccgatcatg agaaacgacc agaaccgatt tttttgtatt ctgtaaatag
    20881 ttttccaacc agcgaatact gttaagatct aaatgattgg tcggttcatc gagtaaaagt
    20941 aaatcggcat cggctgctaa aattttggct aaagcgactt taactcgttc gcctccgcta
    21001 agttctgtta accgtgcttc ctgacggtgt ggaatcccta agccttgcaa gatcgctgca
    21061 atccggtctt ctaatacgta accgcctagt tcttcaaagc gatgctgcag ctcaccgtaa
    21121 cgggccagac acttctctaa gtttgtatct ggttctgtca tcaatgcttc gtagcgactc
    21181 atttgctgtt tgaggtcctg tatctcagcg gcagaatgga gcaagtagtg gttgacggtc
    21241 gcttcggaaa ctgtaagttc ttgttcgacc gtcccgatct ttaagccttt tttgcgactg
    21301 ataacaccgc tgtcgacacc ttctctgccg gtcaagattt gtaaaaaggt tgatttgcca
    21361 gttccgttga taccgactaa ggcaattttt tcaccagctt caatttgaca ggataaatcc
    21421 tcaaataatg gattcccgcc gattgttttt ttaatagaaa ttgcttgtat gatcatgatc
    21481 tctctccttg agaccactca aaaaggccat ggttatccct ggcctgtgtt atttaaactc
    21541 agattatcat gagaaaaata ccttgagatg gccattgatt ttacgtgaat tcaactgttt
    21601 tggacaaact tgtcccgtca acagcaactg taaaaccaat tgcatggcta aatgcgatat
    21661 ggtatcgttg gtaattttca ttacttgtta ttttagtcat gcgcatcata gtattttccc
    21721 tccttcgtgt agttgtcttt tccagtgtac ctgaaagtgt cagaaagttc aactagcgct
    21781 gacatttact taatatttat cgatgtgtaa ttctgtcaga gagctttttt tatctgactg
    21841 agggtaatgt ctcgtggacc acatcattgt attctgagct aaaccagcgt tcaggcgaaa
    21901 tcatatggtg tttgataaaa aactttgtct acatgacttc cttttgccat ttgcttaata
    21961 ccgttgcatt aaacagctct tctctttgga aatctcacca acatcgcgtt caggagtatc
    22021 gttgtggttg caatggcaag cggtagcctt tcatcaattc ttgaatgatt taccgaatgt
    22081 agcacttgcc ttctcgcggt acccgaaccc gaaataaaat tttagtttgg cgataaaatc
    22141 ggtatccgcc atcgatactt catatttttt cgtatcagat tcatcggaaa cttccacatt
    22201 gacgaaccaa taagctttgg catgcttagg tcgttttaaa ccacaccagg gcgactaatc
    22261 gccctggtgt tcactttttt tattttactt gaactcattg actgttttcc ctttatttta
    22321 aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg gttttttgtg
    22381 caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt gtggtcttta
    22441 gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc tgcgtttcta
    22501 ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa tcctgtggct
    22561 cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat aatttgtttt
    22621 ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt cgcattgctt
    22681 tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag cattccatca
    22741 aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag aagttctccg
    22801 ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc atcagaacca
    22861 tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc ctcaagtcaa
    22921 tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat ttactgcttt
    22981 ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat aattacgatc
    23041 agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat acccattacg
    23101 cctaaaacag catatgcagc accgtaaaat tgtaggaatc cttgattttt atcatttttg
    23161 ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac gaataataat
    23221 actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa aataagcaac
    23281 aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga tggtaatctc
    23341 cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag gtgggtccca
    23401 tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac acaagacggg
    23461 tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc gaacatcaca
    23521 gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga gctcgcagaa
    23581 acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt ttatacggta
    23641 atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat aatactgagc
    23701 attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa aatgatgagg
    23761 atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc tatctctcaa
    23821 atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg catctaatgc
    23881 catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt ctgctagtgg
    23941 gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca ctgtacgagc
    24001 cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca gacgccggca
    24061 aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg cagcatccac
    24121 atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag catctccatt
    24181 tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca gtgtaagatg
    24241 aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc cattcaatgc
    24301 actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt cgtctaactg
    24361 gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga ctgccaactg
    24421 gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc ggatcgctgg
    24481 attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc ggccactttc
    24541 taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt catccaataa
    24601 gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat ccttttttga
    24661 atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga tcgcacttgc
    24721 gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga gcatgcgagc
    24781 atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac caacgacttc
    24841 atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat tttcctcctt
    24901 cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa gaacagcctt
    24961 ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag taggtgagaa
    25021 aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg ccagaagtgg
    25081 cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga ttagctggat
    25141 atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat agctatggtt
    25201 taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc tacctttaca
    25261 cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt cctcaaggag
    25321 taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa aatcgtggcg
    25381 gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca ttagcaattc
    25441 ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt tttgagaaaa
    25501 aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac aaaggtgtat
    25561 ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct ttacaaagaa
    25621 aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa ggatctatta
    25681 acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc tttagattgg
    25741 gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa taaagcacaa
    25801 tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga atttgaagac
    25861 ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta tctagcagta
    25921 atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt agtggaatat
    25981 ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga tgaagacgat
    26041 gacgaagctg tattcgagct tcactgggat aatcaacaac tagcagaatt agtagaagta
    26101 aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg agtgacagat
    26161 atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc agttgctaat
    26221 attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga tgttgaacat
    26281 tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc gctgaaagaa
    26341 ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat cttgcctttt
    26401 atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa aaataaagta
    26461 acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata ctatcatccg
    26521 gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca gacagaagat
    26581 cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg cactcatccc
    26641 acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga aacattgcta
    26701 gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg tgcaaaagat
    26761 gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa tatggatatg
    26821 aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg aatcgagatt
    26881 gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat gatcccagat
    26941 gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat tgaagagaag
    27001 aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca aattttccag
    27061 caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat gcagcggtat
    27121 tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga aaactatcac
    27181 cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc attcaatgat
    27241 caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta tctgaaagaa
    27301 attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc gagtggctat
    27361 cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg agcgcaagct
    27421 tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa acgtatgaaa
    27481 cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca tcggcagatc
    27541 gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct agatgattat
    27601 gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc aaatgcagcc
    27661 aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg accagaaggt
    27721 ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact ttgtggatta
    27781 ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc tgctagctat
    27841 caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa agagtatcga
    27901 cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat ttatacaatg
    27961 gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa aaaaacgtat
    28021 aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc cgttttaccg
    28081 aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg gacccggtgt
    28141 cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg agaaagcatg
    28201 cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac cctacattat
    28261 tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc atacagtggc
    28321 tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag atctgagaga
    28381 agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag ggaaaataaa
    28441 atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc tagcaatggc
    28501 tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca tgcgtacgtt
    28561 gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag aaatctatgc
    28621 gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag atacagcact
    28681 tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga cgaagagaga
    28741 agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga cagaagagct
    28801 ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct atcgtaaaat
    28861 gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc gagtgatcgt
    28921 agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat ggaaaccaat
    28981 gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc aatctttgca
    29041 tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa cacaggaaat
    29101 gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag gaaaaaatga
    29161 aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga tactcgaact
    29221 tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag atatctttag
    29281 tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa aaggatccgg
    29341 accattggac tttgcataca gtatccatac agatatcggt aacaaaacca ctggtgcaaa
    29401 agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata tcattgagat
    29461 catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag ttgctactag
    29521 caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag aaaatgtgat
    29581 caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc ctaaagatat
    29641 tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa cagaagatga
    29701 tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca atcgtctgac
    29761 tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag aagaaatcat
    29821 gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg gcgttgtcat
    29881 tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga ttcctggtga
    29941 tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc gagattgtcc
    30001 gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg aatgggaaga
    30061 tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct ataatcgttc
    30121 aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc tcaacagcgt
    30181 tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg gtatccagaa
    30241 tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg tctacagtgt
    30301 acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg gtaagtaaag
    30361 ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta cttgttttat
    30421 taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa aaaattgcac
    30481 aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa gatgtgggag
    30541 gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa ggaaatcgac
    30601 caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa tcattcaatg
    30661 aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca gatatgtcgg
    30721 tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac aaataaagta
    30781 atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact gataaactct
    30841 aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc ggaaaaaaag
    30901 ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga aagaagtacc
    30961 agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat acaacatgtt
    31021 ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt tcaataaaga
    31081 agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa ggaatttcaa
    31141 acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag gtggagtaca
    31201 gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc taatgacacc
    31261 ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag cacttgaagc
    31321 acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta catctatttg
    31381 cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa aattagcaga
    31441 atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca taaaaggtgt
    31501 cgagaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag ctcgacaaaa
    31561 tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca cagatttaga
    31621 tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca tccgtccaac
    31681 ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta gaccagattt
    31741 gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc aagaaatgag
    31801 acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag tagaagtaga
    31861 tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa cagaccatga
    31921 tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag ggaaagcaaa
    31981 agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa attttcgaca
    32041 aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt tttccgatca
    32101 gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc cattcaatca
    32161 gtttccgact tatccgaata gccgagcgct actttacaag aattagatta gcataaactt
    32221 atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta ctataaattg
    32281 ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata atcttcattg
    32341 gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg cttagctcca
    32401 ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag ttgcgcagga
    32461 gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa aatttctctg
    32521 aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc aaattgtttt
    32581 tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa atagggaaca
    32641 aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg ccgatggatc
    32701 aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc tttcaattgt
    32761 tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt taattcgtgc
    32821 aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat ataaaaaatg
    32881 tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt gagaaactca
    32941 ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt agcgatgacg
    33001 acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc ttcggaacgg
    33061 ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa agctgtacga
    33121 actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc tccaacggca
    33181 tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct tatttctgcc
    33241 atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct aaactaaaac
    33301 gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt ttagtaggtt
    33361 ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt acaagagagg
    33421 atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct ggagatggac
    33481 gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa atctttcaaa
    33541 attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca agggtgattt
    33601 ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga ccaaaaggaa
    33661 caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa acagcacgtt
    33721 tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa cactatgaag
    33781 tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg tatgattttt
    33841 atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca atcgttcgtt
    33901 cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa actttttata
    33961 tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa ttccatcaaa
    34021 tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc atggtgatgg
    34081 cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc aattcattag
    34141 gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt ttaccttttg
    34201 aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta cgcgtattgg
    34261 acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc ttagattatc
    34321 taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat gcattggctg
    34381 ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca catacgattt
    34441 ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc gcaggcggac
    34501 gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc ggatttgcaa
    34561 tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc cctgcattta
    34621 atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa gcattgaaag
    34681 tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg aaccgtaaag
    34741 caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg gtgatcggtg
    34801 aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga aaagaaaaaa
    34861 gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa atgactttat
    34921 tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat tagtttccga
    34981 acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac gagaccttgg
    35041 gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg tctttaatcc
    35101 tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat acgtgatcga
    35161 agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa tggccactgg
    35221 tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa ccccgccatt
    35281 tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc gttatttgga
    35341 tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa caaaagcgat
    35401 tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc tagggaaatc
    35461 tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg gacatttcta
    35521 tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg gattcgaccg
    35581 ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc gtcagcctga
    35641 atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc aaacttacac
    35701 agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca cattgccatt
    35761 tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac cggatacacg
    35821 ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat tcaaagtctt
    35881 ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag gcgctgcaga
    35941 tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat tcggagctaa
    36001 ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg ctaaattcat
    36061 gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg acttgctgat
    36121 gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc ggacacgttt
    36181 aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg taactgattg
    36241 gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc atccattcac
    36301 gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag tctatgcaga
    36361 agcttatgat atcgtcttga acggttatga attaggcgga ggttctatcc gtattcatac
    36421 tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac aaatggaaga
    36481 acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg gcggtattgc
    36541 attaggattg gaccgttttg caatgttatt agccggagaa gataatattc gtgaagtcat
    36601 tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa gtatggtttc
    36661 gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat agccgataaa
    36721 aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa ttgaaaaaca
    36781 ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa cattcgaaaa
    36841 ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg cgttgatcgc
    36901 atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg gtattactgg
    36961 tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag taccggtatc
    37021 aacaactttg tatttactga atataccttt gtttgtattg gcgtggttaa aaatcgggaa
    37081 gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta tccatatttt
    37141 accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg gggctgtgat
    37201 gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc tagatatcgt
    37261 gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga tctacttcaa
    37321 tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt tttacagtac
    37381 gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac aaaagaagat
    37441 gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc aaaactgtat
    37501 gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg ataagcagac
    37561 agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag caatgaaagc
    37621 ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg gtcgtttcta
    37681 tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg tagacagacg
    37741 ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa ctgctttttt
    37801 atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt tatgggatga
    37861 tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt aatacccgta
    37921 tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa caaaaaaggt
    37981 taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct gtggtgggtg
    38041 ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc gttccgattt
    38101 ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg gaacaaaatg
    38161 gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat ccggcacttg
    38221 tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg ctgttaaaca
    38281 atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg caaatcaata
    38341 acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat atctggtctt
    38401 tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg ttgatggtat
    38461 ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc ctctccgcca
    38521 tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat tacggtaccg
    38581 acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg ccaagtacgc
    38641 gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct ggaggaatct
    38701 cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat ttcatctatt
    38761 acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg acagcacacc
    38821 ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt ggcaaacgaa
    38881 gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca aaggtgactg
    38941 atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt ctaatcatca
    39001 gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat ggtcgtacat
    39061 tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa ccatgggttg
    39121 ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca ccgaaaaacg
    39181 ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa aaattagcag
    39241 aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg atccttgata
    39301 agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta acggcatttg
    39361 gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca aaagtagtgg
    39421 tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa aaattaaagg
    39481 aacaagattt gttgaaagac actgttctga ttggactagg aacgaatggc tcattctcgg
    39541 aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac tgggtcaatg
    39601 tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa cagatgacga
    39661 aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac catagtgact
    39721 ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac attcacttag
    39781 tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca aacgtgacag
    39841 aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag tttttttata
    39901 tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag tgtgtctgta
    39961 aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct tttgttgctt
    40021 cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt ttttgaagac
    40081 gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa agatcggttg
    40141 tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa tgttcatttg
    40201 ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta agtgatcaat
    40261 ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac taacaaaata
    40321 tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc aattcgatag
    40381 tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat agtaaagcgt
    40441 ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt cgtatgaatc
    40501 tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg cagccctttg
    40561 aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac agataagctg
    40621 tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag cgcgatacta
    40681 cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc cgcctgggtt
    40741 cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac aatcttccct
    40801 tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa agaaaacact
    40861 ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg ttcgtcttcg
    40921 tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac tatttttatt
    40981 aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg accataagaa
    41041 gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag tactattagc
    41101 cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag atgagaatta
    41161 catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg tagctgcagc
    41221 acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag ttctgcgatt
    41281 tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac caagcgtcat
    41341 taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt ttcccagtgg
    41401 gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa tgtctaaagc
    41461 gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt tcaagcgtca
    41521 gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga aaacggataa
    41581 agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat tggacaaaga
    41641 agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt ttatgcgtgg
    41701 gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa acgagggaga
    41761 gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca ggtgtcatgt
    41821 aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg aatgcgtttt
    41881 ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt gattgaagac
    41941 cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt agaggcaatc
    42001 gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt agaattatac
    42061 aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc tatatcagga
    42121 aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta ttcgaactat
    42181 tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa cgtacaattt
    42241 ttctttcttc cttactttga accaattgca gcgagattat attttgaaga tgacaatatc
    42301 cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct atttgattca
    42361 gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa atctgattcg
    42421 gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact tacaccgttt
    42481 gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga taatgctcgc
    42541 tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa aggcgtatgg
    42601 atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc cctgcatgat
    42661 attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta tcagtccgtc
    42721 aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc gaatatgatg
    42781 aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa cggtcgagca
    42841 gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa attagcagaa
    42901 gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa atggctcgga
    42961 gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa aaaattacaa
    43021 cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt gacagaagca
    43081 ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt tgatgggatc
    43141 accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga gatgcgttct
    43201 ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca tcagcagatg
    43261 agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg aaacggaaca
    43321 aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa agaaataaaa
    43381 caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa tctcgatgcg
    43441 aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt tttggtttca
    43501 tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt tttccaacag
    43561 ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga tcatttagag
    43621 caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga accagcagca
    43681 gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga aaaacaaatc
    43741 acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact agaaaattcc
    43801 tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt aataaacaag
    43861 caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg gattgggtat
    43921 tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat aaaagttgaa
    43981 aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat gactacgcag
    44041 aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac aaaaatcaaa
    44101 gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga atccttacgt
    44161 agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga attgaatgcc
    44221 gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct tcaagaaaat
    44281 ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt aaaatatcta
    44341 gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc gctcgcacag
    44401 ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca gctattgaaa
    44461 gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca aattgctcgt
    44521 cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac ggaacacccc
    44581 atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt agaacaacaa
    44641 ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa aagtgaagca
    44701 catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat ggcaacgaat
    44761 atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc atttcctgag
    44821 ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt agatagacga
    44881 aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca agcagaaaag
    44941 ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa tcagctccag
    45001 caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga tagtaactgt
    45061 ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa tcaactagct
    45121 gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg gacacagctt
    45181 gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga acaagaaaga
    45241 atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat ctgtgcattt
    45301 gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta tcatgaagag
    45361 caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac tgagacattc
    45421 ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat cgaaataaag
    45481 caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat ctacgatgaa
    45541 tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca attacagcag
    45601 ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg gtatgtgctt
    45661 caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg tttaacgaaa
    45721 aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa aaccggatta
    45781 gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac cctgtctggc
    45841 ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt gatccagaca
    45901 caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg gtcattagat
    45961 gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga aggaagaatg
    46021 atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca aataagaatt
    46081 gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa agggagattg
    46141 gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca atacttgcaa
    46201 gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca tgcagaagtc
    46261 ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat agattactgc
    46321 caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt ggaactttat
    46381 ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct agaaccggta
    46441 aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa aacacaaaac
    46501 aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat tgagactaat
    46561 cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc attggcatca
    46621 ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta cacagaaaaa
    46681 gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc gtttcaaaaa
    46741 gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca attattaggt
    46801 acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct cccagtagaa
    46861 caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct gcagataaat
    46921 gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc attcaaagtg
    46981 gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt tttctttatg
    47041 cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata tcaacaaacg
    47101 gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt agtgttcaag
    47161 aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga gatcggcgac
    47221 gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga atttattttc
    47281 tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta tggtgaagtc
    47341 atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca ggtgttacgt
    47401 gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta ccagaaaaat
    47461 gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt tttccgcaca
    47521 gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt gcgagagttg
    47581 tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg ttcatggctc
    47641 gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt cctgcaaagc
    47701 ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc ttttgattca
    47761 gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat acgtacagaa
    47821 gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca attggaaaaa
    47881 agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat tcatccagaa
    47941 agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta tcaaaagcaa
    48001 ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt agccgatgaa
    48061 atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa agaagaacgg
    48121 aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg gcaggcagag
    48181 gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa aaaagaacgt
    48241 gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag cttaagacag
    48301 gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga ggcacaaatg
    48361 gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt acctcagcgg
    48421 tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc attgtttgca
    48481 acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc cgcagaagaa
    48541 attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt gttgaaagac
    48601 ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca aaaaactgtt
    48661 tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc tgaagcattc
    48721 aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat ttgctgcgat
    48781 cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca agtaaaagat
    48841 ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca atttacaggg
    48901 atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt ttatttaaga
    48961 ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc tggcgaaaaa
    49021 gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac gggagcagat
    49081 acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc tgctggacgt
    49141 gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc tgaaggaacg
    49201 attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca aaaagtgatc
    49261 caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat gattttaagt
    49321 gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt aagaaagcct
    49381 ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg gattccattt
    49441 tatcccagct tccttgctat cattttttta tttctatttt taaaggttat acaaagattg
    49501 tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa attggagtct
    49561 gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca ctttttgcac
    49621 ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga agcatatgaa
    49681 attctttctc agccagctga aaaatctctt tttctggttt ttggaaacct gttgcttgag
    49741 aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc agtttttttg
    49801 tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt tccttcaaga
    49861 aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct tcctcgtaaa
    49921 ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa tcttttagtg
    49981 attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc attacttttg
    50041 ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata tctgcatctg
    50101 aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga tcatacatcg
    50161 tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt cggttttcat
    50221 tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa aagctgataa
    50281 ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt tatatttcag
    50341 tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat taatcatt
//
LOCUS       DAXLPI010000007        49571 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXLPI010000007 DAXLPI010000000
VERSION     DAXLPI010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.007"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            148..1599
                     /locus_tag="ACF3T5_000330"
     CDS             148..1599
                     /locus_tag="ACF3T5_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFD3630972.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1948..2580
                     /locus_tag="ACF3T5_000331"
     CDS             1948..2580
                     /locus_tag="ACF3T5_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFD3630973.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3168..4607
                     /locus_tag="ACF3T5_000332"
     CDS             3168..4607
                     /locus_tag="ACF3T5_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3630974.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4902..5120
                     /locus_tag="ACF3T5_000333"
                     /pseudo
     CDS             4902..5120
                     /locus_tag="ACF3T5_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            5369..5584
                     /locus_tag="ACF3T5_000334"
     CDS             5369..5584
                     /locus_tag="ACF3T5_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630975.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5801..6057)
                     /locus_tag="ACF3T5_000335"
                     /pseudo
     CDS             complement(5801..6057)
                     /locus_tag="ACF3T5_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6244..7155)
                     /locus_tag="ACF3T5_000336"
     CDS             complement(6244..7155)
                     /locus_tag="ACF3T5_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFD3630976.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7174..8178)
                     /locus_tag="ACF3T5_000337"
     CDS             complement(7174..8178)
                     /locus_tag="ACF3T5_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFD3630977.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8175..10298)
                     /locus_tag="ACF3T5_000338"
     CDS             complement(8175..10298)
                     /locus_tag="ACF3T5_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFD3630978.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10303..12750)
                     /locus_tag="ACF3T5_000339"
     CDS             complement(10303..12750)
                     /locus_tag="ACF3T5_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD3630979.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12737..13126)
                     /locus_tag="ACF3T5_000340"
     CDS             complement(12737..13126)
                     /locus_tag="ACF3T5_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFD3630980.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13186..13689)
                     /locus_tag="ACF3T5_000341"
     CDS             complement(13186..13689)
                     /locus_tag="ACF3T5_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFD3630981.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13702..13926)
                     /locus_tag="ACF3T5_000342"
     CDS             complement(13702..13926)
                     /locus_tag="ACF3T5_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630982.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14030..15940)
                     /gene="ltrA"
                     /locus_tag="ACF3T5_000343"
     CDS             complement(14030..15940)
                     /gene="ltrA"
                     /locus_tag="ACF3T5_000343"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFD3630983.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16686..16823)
                     /locus_tag="ACF3T5_000344"
     CDS             complement(16686..16823)
                     /locus_tag="ACF3T5_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFD3630984.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16820..18004)
                     /gene="mobT"
                     /locus_tag="ACF3T5_000345"
     CDS             complement(16820..18004)
                     /gene="mobT"
                     /locus_tag="ACF3T5_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFD3630985.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18263..18553)
                     /locus_tag="ACF3T5_000346"
     CDS             complement(18263..18553)
                     /locus_tag="ACF3T5_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630986.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18543..19685)
                     /gene="dcm"
                     /locus_tag="ACF3T5_000347"
                     /pseudo
     CDS             complement(18543..19685)
                     /gene="dcm"
                     /locus_tag="ACF3T5_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19761..19925)
                     /locus_tag="ACF3T5_000348"
     CDS             complement(19761..19925)
                     /locus_tag="ACF3T5_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630987.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(19918..20592)
                     /locus_tag="ACF3T5_000349"
     CDS             complement(19918..20592)
                     /locus_tag="ACF3T5_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFD3630988.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20670..22010)
                     /locus_tag="ACF3T5_000350"
     CDS             complement(20670..22010)
                     /locus_tag="ACF3T5_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFD3630989.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22093..22428)
                     /locus_tag="ACF3T5_000351"
     CDS             complement(22093..22428)
                     /locus_tag="ACF3T5_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFD3630990.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22438..22812)
                     /locus_tag="ACF3T5_000352"
     CDS             complement(22438..22812)
                     /locus_tag="ACF3T5_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFD3630991.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22825..23139)
                     /locus_tag="ACF3T5_000353"
     CDS             complement(22825..23139)
                     /locus_tag="ACF3T5_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFD3630992.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23252..26479)
                     /gene="fss3"
                     /locus_tag="ACF3T5_000354"
     CDS             complement(23252..26479)
                     /gene="fss3"
                     /locus_tag="ACF3T5_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFD3630993.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27336..27590
                     /locus_tag="ACF3T5_000355"
     CDS             27336..27590
                     /locus_tag="ACF3T5_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3630994.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
     gene            27754..28671
                     /locus_tag="ACF3T5_000356"
     CDS             27754..28671
                     /locus_tag="ACF3T5_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFD3630995.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            28664..29446
                     /locus_tag="ACF3T5_000357"
     CDS             28664..29446
                     /locus_tag="ACF3T5_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFD3630996.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            29447..30127
                     /locus_tag="ACF3T5_000358"
     CDS             29447..30127
                     /locus_tag="ACF3T5_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD3630997.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            30120..31016
                     /locus_tag="ACF3T5_000359"
     CDS             30120..31016
                     /locus_tag="ACF3T5_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD3630998.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            31228..33150
                     /locus_tag="ACF3T5_000360"
     CDS             31228..33150
                     /locus_tag="ACF3T5_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFD3630999.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            33151..33597
                     /locus_tag="ACF3T5_000361"
     CDS             33151..33597
                     /locus_tag="ACF3T5_000361"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFD3631000.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            33613..33927
                     /locus_tag="ACF3T5_000362"
     CDS             33613..33927
                     /locus_tag="ACF3T5_000362"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFD3631001.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            33953..35002
                     /locus_tag="ACF3T5_000363"
     CDS             33953..35002
                     /locus_tag="ACF3T5_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFD3631002.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            35014..37617
                     /locus_tag="ACF3T5_000364"
     CDS             35014..37617
                     /locus_tag="ACF3T5_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFD3631003.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            37734..39383
                     /locus_tag="ACF3T5_000365"
     CDS             37734..39383
                     /locus_tag="ACF3T5_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFD3631004.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            39699..40634
                     /locus_tag="ACF3T5_000366"
     CDS             39699..40634
                     /locus_tag="ACF3T5_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631005.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            40627..41397
                     /locus_tag="ACF3T5_000367"
     CDS             40627..41397
                     /locus_tag="ACF3T5_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631006.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            41812..42795
                     /locus_tag="ACF3T5_000368"
     CDS             41812..42795
                     /locus_tag="ACF3T5_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFD3631007.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            43116..44153
                     /locus_tag="ACF3T5_000369"
     CDS             43116..44153
                     /locus_tag="ACF3T5_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFD3631008.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(44242..44565)
                     /locus_tag="ACF3T5_000370"
     CDS             complement(44242..44565)
                     /locus_tag="ACF3T5_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFD3631009.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(44552..45259)
                     /locus_tag="ACF3T5_000371"
     CDS             complement(44552..45259)
                     /locus_tag="ACF3T5_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFD3631010.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            complement(45558..46982)
                     /locus_tag="ACF3T5_000372"
     CDS             complement(45558..46982)
                     /locus_tag="ACF3T5_000372"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFD3631011.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            47157..47870
                     /locus_tag="ACF3T5_000373"
     CDS             47157..47870
                     /locus_tag="ACF3T5_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3631012.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            48019..49350
                     /locus_tag="ACF3T5_000374"
     CDS             48019..49350
                     /locus_tag="ACF3T5_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFD3631013.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT        15345 a         9312 c         9315 g        15599 t
ORIGIN      
        1 ttaaaggcag taacaggatt aaacattgaa acataatcgt tactaaatga aatcaagaaa
       61 tatactgtat tactcaattc attgaaaata tttatcataa tttttaatag aaaacactat
      121 taaagagaaa aaagagaggt aaatcaaatg aaaaaatctt taaaacttgc atcagcagtt
      181 atattaatgt cccaactcct tatagcaaca cctttccatg tgttggcaag cgaaaattca
      241 acatacaaaa ttgcaaaaga aagaaccgag acgactgaaa tgagtaattc cgatgtaaca
      301 gcagagagta gtgaactttt gacaacttca tcaagtgaaa gtagtacgac ttcaaatgcc
      361 gagtcttcaa caacttcatc aagtgaaagt agtacgactt caaatgccga gtcttcaaca
      421 acttcatcaa gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcgtcaagt
      481 gaaagtagta cgatttcaag taccgaatct tcaacaactt caagtagtac aagtgaaata
      541 acttcttcga aagtgacaaa accaaaccat atagaatctt caaaaaagga agcgccaaaa
      601 acggaaggac aaacaaaaac tttttcgaca ccacagcaaa tagaatcaac agaatctttc
      661 caatcacaac caatactatc tggtaatgaa gaaattcatt ttgacaaaaa tgaatctgta
      721 gaaaatttta ttatgaaaat tggtgagtct gcgagaaaaa ttggtcagaa atatgattta
      781 tatgcttctg ttatgattgc acaagcgatc ttagaatctg ccagtggtca aagccagtta
      841 gctcaggcac cgaattacaa tttatttggg ataaaaggaa cttataatgg aaactttgtc
      901 attatggtaa caaacgaaga tttgggtaat ggaacgcttt atacgaccca atcaaaattt
      961 agagtgtatg aaaactatga ggaaagcttt gaagattatg ccaaattatt aactaaggga
     1021 attagtggaa ataaagattt ttatgcagga gcattaaaag ctaatagtaa aacatatcga
     1081 gaagcaacta aattccttac aggacggtat gcaacagata cacaatacta tttgaagcta
     1141 aatgaattaa ttaaaaccta cgatttaacc aactatgata aagaagttaa aggtccacaa
     1201 atgagtaaga ctggatacca agtgcctatt cataattatt caataaccag tttgtttgga
     1261 aatcgtggaa atgaatttca ccgaggactt gatatggcag ctacacaggg tgagccgatt
     1321 catgcagtta aagctggtaa ggttgttaaa gcagagtttc actactcttg gggaaattac
     1381 gtggtgatac aacacgaaga tggatcaacc gcgttatatg ctcaccagca acaataccta
     1441 gtaaaaactg gagataatgt tttgcaagga caaataattg gctatgtagg aagtactggg
     1501 aatagtactg gcccacacct tcatttggaa atttgcaaag atagtagtct ttctcagagc
     1561 atgttagttg atccgaaaac cttattattc ggagactaaa aaaggtaatc aatctgacaa
     1621 agtaacaaga aaatggttgg attcttagac ctttgatcag gatattgaga ctggaattga
     1681 tatctcgatt acgatgggtt tgacaaataa aacaagtcca ttatcgaata tcgagtgtat
     1741 tattgccgtc tttatgctca tattttgagc aggtttcact agatgaaaac aatttatcta
     1801 tcttgaggat ttcgtgtacg tatcgttcag tcttgtattg taaattagtc ataaaatcag
     1861 actaagaaaa attggaaata tttttttctg atttattaaa agttaattga aattatatgg
     1921 tacttctttc ttgggagggt aggtgcagtg aaggaaagat atggtcacct tactgttgtt
     1981 gaggagatta ctcataatgg atataaaaaa tatcgttgcc tatgtgactg tgggaatgaa
     2041 acgattgtat atcgatcaaa cttactaaca ggtagaacca aaagttgtgg atgtggaaat
     2101 tataaaaata ggcataaata taaaaatttg acaaataaaa cttttggtaa gttagttgca
     2161 atcaagccaa ctaaactgcg aagtgaagga actgttgttt gggaatgtca atgtctgtgt
     2221 gggagaaaaa agttagtatc ggcaaagcga cttctaaaag gtgatgtaaa aagctgtgga
     2281 tgtttgaaat caaaaaagca tcacattgaa aataaaaaat tcggattact tactgcaaag
     2341 tatcctttgg acaataactg ttcatggaaa acaaaatggg tttgtcattg tgagtgtggc
     2401 agtatgtgtg agataagtta tagtaactta ttttttggac atactcaaag ttgtggatgt
     2461 ttaaaaaggc aagaaacaag gacatgtgtt gaagggacag taatagagtg tctaacgtca
     2521 aaattaccga aaaataatac aagtggtgtt aagggagttt atcgttctaa agagaaatag
     2581 attgcgtata taactttaaa aaaaaagagg tactatttag gatcgtatga ttctattaag
     2641 agtgcagctc aagctagaaa agatgctgaa aataaattgt ttcaaccttt gattgacaaa
     2701 tatcgaggta atcattaatt attgtggata ataattaaca gtgatatata aacatctata
     2761 atatattatt catactattg atatttagta taatcctatg gaattgaata tcatagcaat
     2821 gtatttgaaa agcaaattgg aaaacaaata ggtgcaacac cataaatatg aaaaaattgg
     2881 gaaaaagtag aatggaaatt tgcttacgtc tcacgagata gctcacacta ttaatctgaa
     2941 gaacaatgaa gtcgactttt agaaattgct agaagaaagt aaagcgaatt atcactagtt
     3001 actatagagt aagttcaaat aaagcatctc caacttacat ggttacatga ggatatcaaa
     3061 tcgaaattac tcaagcttcc tatggctaga gtcgcgagaa gttgttgggc agtagacata
     3121 ggttatattg ttcttagcta cacgatcatt agaaaggaaa tcctattatg tatagttatc
     3181 aagaagatgc aaaagtccaa aaaaatcgct ggtggatttt gattgctata tctatgttta
     3241 ccttcatgtc cacattggat ggcagtattg tcaatattgc actacctacc atctcagatg
     3301 atatgggcat accaatgaat caagcggaat gggtagtgtc cgtttatctg atgattgtct
     3361 gttcttgttt gttgctgttt ggtaaaattg gcgatagccg tgggaaaatc aaagtatttc
     3421 ggattggtac gctgatcttt gtcttgggat cgctgttgtg tgggttcaat cattcgctag
     3481 gtattttatt gttcgcacgg gtggtacaag ccattggtgc tagtatgact atggctacaa
     3541 atgccggaat catcacggaa gtctttccca tacatgaacg tggtcgggca ttgggagcaa
     3601 taggtgcgtt tgtttcatta ggctcgattg ctggaccagg tcttggcggc ttgattttgt
     3661 cccaattttc ttgggcgtac attttttgga tcaatgtacc ggtgggaatc gtaacgatgt
     3721 tgattgctga aaaattctta ccaaaagata tcaccatgag cggccataca atcgatggag
     3781 ggggctttgc tgccattgcg gctacgatta tcagcttttt tggagcaatc tttttggggc
     3841 aagaaagtgg ctttttccat ccagtccccc ttgtactgtt cgtaatttca gtgattgcct
     3901 ttgtcagctt tttaaaagtt gaaaagaaag cagcctctcc attgatttct ttcggtattt
     3961 ttaagaacaa gatttttacc atgagcttga ttacagcctt gttgattttt tcttccaact
     4021 tctttgtcaa tgtagtgatt cctttttatc tgcaaaatgc tcgcgggttg agtcctagct
     4081 atgctgggct gttgatgatg gtctttcctt tgatgatggt cgtcggggcg cctttaagcg
     4141 gctacctgac cgaccgggtt ggcccggaga ttttggtatt agtaggcttg ggcttattgt
     4201 cggtaaccca ggtcatgtat atgtttttgt cagtggactc tcctttgtgg ctatacattg
     4261 tcgctactgc catcatgggt gccggcaatg ccttgttcca gtcccccaat aatacgatgg
     4321 ttatgagctc ggtcactatc gaaaacttgg gactggcagg ctcgatgaat tcctttgccc
     4381 gaaatttggg gatggtcagc gggatttcgc tttctacgac ggtcttatat caagcgatgt
     4441 ctgctaagat gggccagcaa gtaactaact atatcgcaaa tcggccggat gtctttttat
     4501 atggaatgaa gattaccttt ttaggcagct ttgttttgtg ttttgttgcc tttttcctga
     4561 ctatctggcg tatcaaaaaa caaggaggta ttcagaaaaa tgtctgaaaa aaccactgtt
     4621 gaagaatttt tggaggcctg cgtatgggtg agtgggattt ccatggatga tggaagatga
     4681 agtgaacttc taaaagccga aaaactcaag tattatcttc aaaatactag tgctcaatga
     4741 gatgcttgat ttgatcacaa gaagactgcg aaagccgctg gcaaccgcaa gctaggtatc
     4801 ccaccgatgg actacgtttt agaggctact ggttatgaat acggagccaa cgctctcatc
     4861 tgcataagaa tacatcaccc agattatgta aagattttcg atacttctgt caaaaaatca
     4921 ttaagataga taaatgattt ccgtctagtc aaattggttc agaatgtaga cacacagatg
     4981 gtaagaagtc actcacaatt cgagaatgga cttgtcctat ttgccgtata catcataatc
     5041 gaaacgtcaa cgatagtatc aatattttga ccaagggcta tgaatacaag cattgcctta
     5101 gaaaacatca agaaccttag gaacaacggg gatagcttgg ttaataagag ataccgctgt
     5161 ttaggcttgc aagtatactc ttttatcaag aattccgtgg ctttagtcat gagaggctta
     5221 atactatgaa aattttcttt agcatttttg atttaggttc cttttctaat tataataaga
     5281 tgaccactac agtaacctac tgaaatatat aaacagccac tgtagtacca ttttcaaagt
     5341 agtctatgag aggagaatct tcattcttat gaacgctgga attgcggata tgaatttaat
     5401 aaaaaaaacc cttaatgatt ttacttccaa ttcaatatca aaaggtactg gtatcaattt
     5461 aagcacaata aaaaagctga aaagtggtga aagatcagtg gaaaagctga atcttttaga
     5521 tgctataaaa ataacggaat ttgctatgaa aaatggaaaa gctgagattg aaatatggag
     5581 ataattatcg tctagtgaat tacttgccac ttagctgaat ttaacgatcc ttggtgcttg
     5641 aagggggaga ttcttgggaa tagagcatac ttgcaagcct atgtctttac taacaacgat
     5701 atctcttatt aaccaagcta tccccgtagt tctaaggctt ttacttttat ctaagcaata
     5761 atcgtattca aaggaaattt ttgaccctta gaaagtctca ttttatcaga cgtgagctta
     5821 ggcattgctg aattttgtta attcggtttt aggttgtgct ttgagcataa tatgaaagta
     5881 ttctttatca cgatttcatt catctaaggt aatgtgatgt gattctgaaa ttctttcaaa
     5941 ggcagttttt gcataagctg acattacatc atcaatgaca tgtttacaat gtttcactac
     6001 taaaataagg gggtaaccaa gaagaaaaac tgaatgatta tttgtatcta attccatggt
     6061 atatcaacca tttcctatat agactgatta tgacatggat taaaataaaa aggcactatg
     6121 tcttaattgt cggttttcga gttaccgaat ggcattcatc acccacctat agaggatggg
     6181 cgacttctgc ctgaaataag ttacactact tataacttct aataaaatta gattgagtca
     6241 ttattattta acatcaacga ttttccagtt tccatttttt tgtaatacta atttgtactg
     6301 aaagtagcta gtggtttttg atacattatc aaggtatttt atctttgtac tcactagaaa
     6361 attttttccg ttttgtagat aaactggatt ctctaactcc gaaaacttta agtcaatatt
     6421 gattggtcgc atcgcaccat tttcaacata atactccaat tctttttcag aagctgatgg
     6481 ataaagttta aaaaatgttt ctaaaaaatc ggtgatttct tccgttgttt tggcatcaat
     6541 ttctgaatcg ttttctactt gttttgcttc atattttgcc ttgtctggct ttccaatcat
     6601 tgttggcaaa ctagttacta aatgattcct gttttcatct tcaaagattg tcactcgata
     6661 tgcagaagaa attgattgcg tcttttttcc ctctgtaata tgttgctcca ctaaaaatga
     6721 aacgacaaat tcttccgagt gtttttcaac atctagaatt tttacagatt gaacttcgga
     6781 actcgtggga atatctgctc gaaccatatc ttggattaaa gcgagaccgt tttctgtaag
     6841 gtactgttcg agattactca ttctcttttc aattgcttct ttgttatttt tccatgaaaa
     6901 atattctttc gcaaaatcct ctgtaaaact ttcaacgcca gaagtattca ctaccttctc
     6961 ttttataacc actttttcat gagttgtatg ttgatcaatg gcggtgaaat ttttataaac
     7021 gccaaaagca agactacaag acaataaaag ccaaagggca agcaccattt ttcgatgctt
     7081 acccacggat acaactcgtt cttttttctt ttttgggctc ttttcctttt gattccgttc
     7141 aatttttatc ttcattcttt ttcctctttt ctcttatttg tatcggccag ctccgataag
     7201 atgctgccgc caatagtttt ccgttagatt cgtccacccc actggatctc ctgcatgaaa
     7261 catgcgattc tcccctgcgt aaatgcccac atgggtcaca taagtaccgg catcataggt
     7321 tgaatgaaag aaaactaaat cacctggttt ggcatctttt agatcaatat gagtcatggc
     7381 atcatattga tcctgagcca cccgaggaag tttcaggtca actttggcat aacaccattg
     7441 ggtcaagcca ctacaatcaa accccgtttc tggacttgag ccaccaaaca cataggccgt
     7501 tccttcatac ttaaatgcct catcaaaaat ttcttggact attttgtcat caaactgagt
     7561 tgtagttagg tattgcttca ctaataaaca gtaaaacata ttgccataat tataccgcca
     7621 cccaccattt tccttaatcg cgattgggtt ggggtaatcg actttttgac cacctgattt
     7681 ttcctttgaa aaagattcag ctaaagcaaa actatgcttc ttcccgtttt tcccgacatg
     7741 atctagatac ccgccaccat agttataggc ctgaataata ctatcttgat cacaccctag
     7801 ttctttggaa cgcttaacca aagacgcaaa atagagacag ccttgtttga tggatgcctc
     7861 ggtacttaat gaattaggtg gtaatccggc tgactcagaa ctttgcataa cgtcttccat
     7921 tgtgccacca gattccactt gaataatcgc caaaagaatt ggaatatact ctgttatatc
     7981 cgattctttg gcatactttt ccaccattgc acggtgtttc aatacttctt ctgagacact
     8041 agttccgcca ggagaagtgc cagaactacc atttccctca ctgtcttcac caaacaacaa
     8101 acccacacac atcaaaatac ccataaaaga gatggtcaga aaagttaatg aaataacacc
     8161 cactttcttc aagttcattt tttctcacct gaccttgatt ttggacgatg gttcattctt
     8221 ttttgtactt tttgattggt ctgctttggc agagtggacc tctgtttcac ttgttggaaa
     8281 gaatttttct tacttgctga ttggcttttc ccttttgaaa tgatttcttg actagttagt
     8341 tttcgtttga tttcgggatt cagatttgct ctcacaatcc gctttggtgc ttcttttttc
     8401 agcatcatat ctttctgagg ttgagctgct ttatctattg gaatattcgc ccttcttttt
     8461 ccctgacgat ttgttgcttc actcagtatc ttgcgttttt cgtccatttt cgcatttctt
     8521 cgttgtcgtt gtttttctcg caaatccact cgatccgctt tttcttggaa cagtccgcgc
     8581 ttgaattctt ctggtgcttt tttcgacttt tcgattcctt tatggagatt gtatttcaaa
     8641 ttcgttggcg tattccttac ctgatccttt acttgtttag tcttatcttt caccaaagct
     8701 tgggtatcca aaactttgcc gatttttctc ccagccaaat tcattcggga ttgtttctta
     8761 ttatttgaaa ctggattctc cttagttaag tcagatagta tttcgtgatc gttcggtaat
     8821 cgtgaattct ttcgtagaga agaaccggat ggcttaaatt tggattttga ttgttccttt
     8881 gccaacttat ttccgactgc tgcaccacca ccaaaagcca aagctcgtcc cacatttcga
     8941 ttgagttgcc gcaattttcg attcattaac atttgcggtt ttcgcatgac gcgtcttccc
     9001 attgactggc tatcattact ctggaggttg aacatactca taatgtcgcc caatttgaaa
     9061 tagataccgg caaaggtcac aatttgcaga aaagcaacca tgaaaaatgg ataggttgcc
     9121 gaaatattga aaaacatcgt ggagatgcta aatgcagtcg taatcaccaa agtaacaccc
     9181 gctcgcatca taatcgtatt gaataaacga ataatcgcct tctttcctaa actttcataa
     9241 gtcggcaaca tggacaataa gaaactaatt ggcagaaaca tcgcaaagat aatgaagaga
     9301 atttgcgaga acaacataat ccctgttaga agaaaaacaa agaaggaaat gccaatattg
     9361 aacaatacca aaaagaccac ggtccctaaa cggttcatgg tcttggtaat actgagattc
     9421 gcattgtcat tgtcttcaat ctctgccttg accgcttctt cccgatcttt gcctttgttt
     9481 tcatctggac tcacagatag aatcttgttg acccggtctt caccaatttc ttctatgttg
     9541 gaatcatcaa attgcagtag taaccaaggt tgttggactt gaatcgaaaa caaactatcc
     9601 cgaatcaaat ccacactatc ttttccttgg ctttctgaat tgggtacaac gatctcggtt
     9661 ccaagggtaa gggccgcttc actaacatct gagctaaagt cattgatttt tgtgatatag
     9721 gtaggcgcat aagcaatgaa cgaacccgat agtagaaaaa tcatcacaaa attaatcacg
     9781 gctcgaactg ctttcgtggt ttctcgtttt aataatccgg tatacgccac atacaccccc
     9841 agagctaaaa tcatcaataa tagaaaccca aaataaaatc cggaggtctg gagtccattt
     9901 tcagtaatac cagccaatgt ttggatattc tttccaatac tttcagccgt atccgaaatg
     9961 aaatctaatt tgtaagcctc ttgaaccaca taacccgtgg cgttcgacaa atacaaactc
    10021 accgtccaaa tgaaattagt aatggcataa agaccataca tcacgctttt ccctaagcca
    10081 tcaccccaat tccaaggcaa ccaatcccaa gagctatcca caaagaaatc tagttgatag
    10141 ttgttcagtg aatatttgga atatagattt cctgcttcaa ctgtatcatc aactagccct
    10201 gtcgcctcgg ctaccgtacc aactaaactc aatagaacta tcagagtgag tatgctaata
    10261 aaaacgattc ccacaatccg aaaaagtttc tttttcatcc cctcactcca ttctctcttg
    10321 aacaggtgga cgggtatcaa aggcatggaa gagttcttca aagactggat gtacctgaac
    10381 cacgcctacc cgaccataca aatcctgcat caagcattgg ccattttcca aatctcgtag
    10441 gcgtttttga ttgctttcat cttctcgatc caagccaaag aattccaagg ttttcttgat
    10501 ttcatgaata tcggtactgc gaaacgcaaa cttcaacccg atattattct tcaagcgttc
    10561 atccagtaaa tcatcactat tttgcgtgac aaagtaaacc ccggcattca tagcccgccc
    10621 agcacgaacg agcttattcg ataatgtttt cccttgcgct acttgaagaa aactccacgc
    10681 ctcatctaag tctacaattt tgaagatttc tcgattacta tggataaagt ccaaggcaaa
    10741 agtggaaatg acgattagca tggaaacact aagcaattcc atggtggtat attcttcaaa
    10801 agtggtttct gcatctggta aaaccaaatc cgctacttga atgatattta actgcctatc
    10861 taaactaatc gactgggtaa tcgaaccatc tgagaaaagc aaatgggcaa aatcataatc
    10921 ggtaaaggac tcgatgtgat ccgcgatatt ttccgcaact ggtgagcctt cttttcgaag
    10981 ctcattaatt acttgtaata atccccgttg attgctttgg gtaaccgtcc gaatggcctt
    11041 acgcaacact ggaaacttct ccccatctcg actagaaatt cctgttaaaa aggtcagaat
    11101 atcaattgct agactttcag aatccttctt tttctccata atcacataag gatcaagtaa
    11161 tccttgattt tttggcttac tagttaagtt gagaatattg atttcatccg caatttctgg
    11221 caaggtttct ttccagccac cccgttcccc ttttggatcg acaatcacag cttgtccacc
    11281 aaaaaggacg gcgtaataaa ctaagagatt gttactaaag gactttccgc caccgagtga
    11341 accgaggaat gccgctgcta gcgcattggt aactgaatcc ttgacccctt gtgaggccaa
    11401 tgccggtttc aggtaaacat tgcgccccgt atctacgtta taaccaaagt aaatgccttc
    11461 caactcccct aacatctgag tggcgccaaa accaagtccg gcgagaaaat cagacgtaac
    11521 atactggata taatcattca tgtatcgctt actagaaggt aagaattctt catgtagccc
    11581 catcatatcg ccaaaaggcc ggactagttt tacattggta tcatcataaa aatctagcac
    11641 ttcatcacac cggcgcttta gttcatctac tgattcggca cttactcgaa ccacatagct
    11701 cagtttgtac atggattctt tggactgatc caaggtagtt tccaattcat ctacggaatc
    11761 caatgcatct aagacattgg aatttgtttc attgtcagat tgataggcat ggttgtccaa
    11821 gtcttttaat tcctttttct tattgcgaac ggtagcaagt gcctttttgt tcgtcacaat
    11881 ctccacattc atactagtgt caattgggaa tgtaaattgt tgttgctggt aatagaaaag
    11941 ttctgaggag ggaaactcca tttcccccac aattgtatta attgttaagt acgctacata
    12001 tgattcatga ttctcatgtt ccatgcataa gtaccggggc ttttcttcaa tcaaacaacg
    12061 actgggacgt aataagtcgt aacgtttcac caaagtttcc gacttcagct ttttctttgg
    12121 tagctgaaat tcatattctt caaatggtgt acccttttcg ccatagatat gttcaatcaa
    12181 atacatcaga tcactcggtg tgactcgtct gactttaaat cttcgagcca atttactttc
    12241 cattagtttt tcaagcttgg tatatcggcg aatttcttca ttggataagg aaacaaaatc
    12301 gcccatcaaa tgatgattca ctccatagac aaattcttga aagccggaga agaaagattt
    12361 cttgagattc tttagattga cctcttcatc tgtcgcaatc agtttgaagc caataaaaaa
    12421 gcgataatca atctgactat cgccaatcat ggatactaat gcctctgtct gtaaatcaat
    12481 tcgttgtttc gctacttctg ttaatcgccc ggtaatttcc ttttttgatc gttcttgcgt
    12541 ggcacgaata ctactttctg tggcaatttg taaggcatga atcttgcctt cacgattttg
    12601 cgcaatcaat tgacgaaagt tatcgtggac ttgatatttc tgttcaggtg acaaaaagga
    12661 gtaattgtaa ggtattaact catagtaggc aaaacattct ccctcttgat taaagacaag
    12721 attattttca atatacttaa tcgggtacat agttcacact ccttaccaga gtaatgggtt
    12781 cgtcccatcg tttcttctgt tctttgactt tctttcctgc gaaagtgacc ttaaatcgta
    12841 agtgatagct aataaaagat cgtaaaaatc ccaatggttt cttaccgtca aacgtttttt
    12901 gcgacatgaa ccaagtaact gccaccggta tacctaagta ttttaaaaag gccccctcta
    12961 tcatagatag tggaggcaca tgggcaaata cgatgacaaa cagcaaggat aaaacaaacc
    13021 aagccatctg gttaaaagtc acaggaaaag gcagttgaaa gtcattaatt gcataaatga
    13081 ccttttcaac tgcccaaata ctggtgtagc tttttatttt cttcatcaaa tcattccttt
    13141 aaaaagaagg atagtttaga aatcaactac ccttcaataa ttttgttaat atggatactc
    13201 aaaaacacct cgactcgtta ccaaataatt gcctccaatc tccaagtctc taccacacgc
    13261 atcaaaatca atatacgtct tcaagtgttc tggtacttct ccaaaatcac cagcttctac
    13321 aatcaagaaa tatgctacat cactcatatc atcgcaatct ggataataga taatatcatc
    13381 cacatgctct accatctcct caaaagaatt gaagaaagca tgttgaatct cagaagcaac
    13441 ttcaccaact ggcgttcctt caagttcctc cgctaagcca cacaaccgat taatttcctc
    13501 aattggcgta tactcatcaa tctcaaaggg aagttcataa tcatgaattg catattcctc
    13561 gtattcgtca tttaacccaa ttcgttcttt cacttcatca aaatcgacag gtggggtaaa
    13621 ccacgccccc accaattcac cttcgttata tttcccgagg ttcgcaatat aaacacgcat
    13681 ctgttccatc gtgcttcccc cttacgcacc gacaattcgg ttaaacaaac tgagtaagac
    13741 atccttcacc ccagaagcat taaagaccaa tccgaccgca atcaaagcaa tcaccaaaaa
    13801 gccaatcagt ttggaaaact cccgcttaaa tcctaaaaac aaaccaatga ccacaattgc
    13861 cagtaagact aaggattggg cattggttaa aaaccattga tatatggata ggtaagttcc
    13921 tgatattata aatatctcaa acttttcccc ccattcacac ggagcgtgcg actttcaccg
    13981 cactccgcgt tccatcgtgt ttatattgaa aagtatttat gtagtagacc tatatcaagt
    14041 ttaattcttg attgccagct aatactctat atttatttag tcttatgagc tggactttat
    14101 ttagcttcaa tagagctata tatttttgta ttgtttcttc tgttttagcg tgtattagac
    14161 ggtgtaccgc ctcttttacc aaaataagat ttttataatc gtcatttcca ccagctccag
    14221 ttggtacaat atgatgacaa tgtatttcat tagcttgttg aaattcttcc agagttatag
    14281 cacattttcc atgttgagca gaaaatagcg aaattcgatt gtctgcgtat tctattgtgc
    14341 gattttccaa agataatttc attaactggt acatcagtaa tttatcaaca cttagattgg
    14401 tgtggataaa ttttcttccc tctagagtgt agcaattcgc taaatacacc tttgccattg
    14461 gttttctgtg ttggatatat ccaacaggga aaataggttc gccctcgcca gcaacatacc
    14521 gaagcatttt agatttacca tatcttgttg cctcctttgt cgtaagttgt ctgcctgttt
    14581 ttctcaatct gccccttttg tttgtaccta atctgttagt gaagatagcc attacagctc
    14641 tattcagttt catacaatcc agatttacat tcgttgcaat ctgataataa ttttgcatac
    14701 ctaataccat tgaattataa agtcttgttt cttctaattc cgttttccct tttgagggag
    14761 aagcaatttt ctttgcctgt gataccagct tgtctttttg gtttttgaat tgtttatcac
    14821 ttatatgtga tttaacaact aacttctttc ttctcggtat cattttaatc ttgaaaccaa
    14881 gaaagtcgga gtatcttttt ctaacattta caattcttgt tttttcttgt gatacttcca
    14941 attttaatcg ttgttcaatc cattgagtga ttgctatttt ggctttttca gcactttcct
    15001 tgtagcggca gaaaactcta aaatcatctg cataacgaac catatacatt tcctttaact
    15061 ttgttttctt catttctttg taagcgttgc ttttacatgg gctaccgctt ttattgatat
    15121 gaaccacata gttttttgta acaggatttt cctgccattg gctttctacc caatggtcta
    15181 actcattaag aacaatgttt gctagtagtg gggatattat tcccccttgc ggagttcctt
    15241 tctctggata tgttattgta ccgttagtca ttttgattgg agcttttaat atccttttta
    15301 aaatgaatat caagtgcttg tcccgtattc ccatagccca tatctgtttt atcagcttgg
    15361 aatggttcac gttgtcaaag aaacccttaa tatcaaattc aatcacataa tgtagctggc
    15421 tgatttgtag gcggttatag gttgctttta ttgcattttc aactgaccta ttaggtctaa
    15481 aaccatagct gttttcacta aatttagctt cgcagattgg ttctaatact tgttttatac
    15541 attgttgtat aagcctgtcc cacatacaag gaatacctag aggtctggtt ttaccatttg
    15601 gttttggtat ttcttttcgc ctcactggct taggtcgata cccatgtgga ctgcctgcaa
    15661 caatgtatct tacttttttg acaactgttt ctgctggcaa tttgccaata tctttaatta
    15721 ttgtaccgtc tgttcctgct gttttactgc ctgcatttgc tttgatattt cgatatgcca
    15781 gtaatatatt ttctctcgat aggataatat ccataagatg agtgaaaata tctccattct
    15841 tacttttggc atataaatca tcaaaggtgt tttgcatttc gtaatattca gcgtgtctta
    15901 aatcttctac acacaatgtt tcatttttat ttttaggcat aaggcttcac tcctttactt
    15961 gaaattgagt gagctttttt atcttactcg taatccttat tgaaacaaca taatactttt
    16021 ctaatatata cacgacttga gggcattcct ccattctcat tacaagaatt tcttaggttc
    16081 gccctctgct tttcagcatt agttcaactg cttattgttc ttcgtcagtt tattctcttg
    16141 atgagaaccc aatacctttc ctcgttccga taatcttatc tatacatata tatccttagg
    16201 tcactagcta taagcctgct ggcttgatac cgcctgtaac gttatatggc atttcataac
    16261 ctatattctt actttgccac accagctacg atttctcgta cctttgcatt tctacaaagt
    16321 tcaaatttag acccgtacat tcgctgtttc gtcagttcct tatagaacat tctaaccata
    16381 gatattttat agacccccga cctgttggat acgctaccca cctctggata gttttcgata
    16441 gaagatatat gttcatcaac tattacggta tctttcagcc gtctttaccg agcttcaaac
    16501 attctgagtt tcctcataca atgcttgtcg gaggctcagg gcaggcgttt cagagcgtta
    16561 cttcatcatt ccacctgtca gattatcagt tctcctaata accaaacttt ttcgttcttt
    16621 tattagtgcc ttagcttttc actaaggaac gagtcgcaca taaattttgt ccgaaattca
    16681 tcttgtcact ttcctttctt ttccattgct cgatccgctg ctgcacttgc attcatcaag
    16741 cacatcacag taacaccaag catactgcca ccaaatacca aaaaaatatc ttttacaatt
    16801 cccatgacga ttcctctttt catataatca aattttcaat tgccatactt tgttgctcca
    16861 aaatcttttt atgtcggtct gataagcgtg cattctgtac catatcctgg atataggttg
    16921 tccgattcaa tcgatccaac tccttagcca ttttccacgt aggggcgact tgtcggccta
    16981 gccaattcaa ggttcgttca atggtatacg gttttggctc ggaagttaat tgaatctctt
    17041 tccgattccg tccaataaaa cgcccccatt gttcattcaa gggccagttg gttcgccttt
    17101 taccttctac tttatccgca aatcgcaagt aacgattgat aatggaaaac gtggtgctct
    17161 caatgctacg accttttaac aaatcttgaa tcgcatgaaa agcacggtca tttttcagtc
    17221 gaatctcaaa acgatttttc gtctcagttt ggtctaatgg aatccctaac ttgatatatt
    17281 gttcatagtc tttttcatac aaacagaaat aaacctcact cttgagactg ccgatatata
    17341 aggtattgcc catgccgtcc ttttcatcag cctttaaaag ttccccggaa cgataacttt
    17401 taaaggtacg aaacaaggag atacattctt ccttctgaca ttttttcgtt agctcaggta
    17461 tatccaatag ccccactcga tcattgattg ccaagtccaa gcgtttcatc acgccaccgg
    17521 cctttagaca atcctcgaaa aagtcgtacc agctacgctt ttgagcgagt aaaaaagttt
    17581 caaattgtcg gcaacctcgc ccctttaatt ctaaaagcac acctttatct tcttcatgag
    17641 acaacatcac cacaatatca cccatgacat actgttcttg ataggagtaa aaggcataat
    17701 cttcatggag catatactca aatttcaaat ggagaatttc ttgaataatt tttcgtgcat
    17761 ccgttgtggg aaagcgaatc cttacatagt caaacagtaa cgtcaaaggc aaatcgggat
    17821 taaagctttc cagttggtca aaaattttcc tcatggtttt tatagatgcg gttacgccac
    17881 cattttctat ctgatttaag tacggccgac taagtccaca agccgtagcc aatttatttt
    17941 gtgaaacgcc gtattcgttg cgtcggtctt ttaatagccg ataatctaaa ttccgttgtg
    18001 ccaagttctc acctccaaat tcaaggtgtt aactttttca aatgagttaa cacctataca
    18061 aattacttac tatctttgat acaacaagca ttcttaatgg ttgcttactt attttttgtg
    18121 tttttgtacc cccctgttag atacgggggt gagacatcat ggtcggctaa cgccgtccat
    18181 gaacaggcac agcaccacgg aggatttctg cttcgcgaat cctccgcggt cagcgcctaa
    18241 cagtcagttg tgttggttta ggttactcat ctgtaatacc atttacttcc aactctgctt
    18301 tcatttctaa caagtattga cgaatatctg gttcttttgc ttggtccgca atccgctcaa
    18361 attctgcaat tacttgattt atttcaattc gagcatccgt ctcctcattc attaaatatg
    18421 gatcgccagc gactttgaca ccattcttgc gaatatcaaa ttttaaccgt cgcaattgtt
    18481 cttctaattc tgggtctgac tgaatctttc caatcaccca ataaatttct ccaatcaact
    18541 catcataaac catgtacatc caactctttt aatcttctgg caatatcaaa aatgaccgga
    18601 actgttacag agtttccggc ttgcttatac aactgactat ctgaattgac ttgagaatct
    18661 cgatcaaaag cccaatcggg aaagccctga agtcgccaac actctctagg tgttagcttc
    18721 cgaatttgat actgatccgt gacgatcgct tgttggtctc ctgttagtaa ggtatgaaca
    18781 ctctgttttc ccagacgacc tcggcgactt gtggaattag gaaaagccag attcacactg
    18841 tcttctggct tcacctcttg atattctgtt tcattttttc gaatcatgat ttttgtacca
    18901 ccttgtttcg ttgacaaggt gggggcggtt ccagtaactt cataaactcg attaccttgt
    18961 tcaaactttc ccggtaatag tcctgctaca gagattccat cccttgctgg cagtgcgatt
    19021 ttgattcctt catcacctgt taaggttggc gctacgccat ctgaaccata gacttgtcca
    19081 cccattcctc gattggaagg attgatattt accaaatttc tgatgttttg atcagatgtt
    19141 gaagacggtc caatgatagg aaatacttgt tcggtacatg cgtctcgaga atgtccgata
    19201 aggaatatcc gctcccggct ttggggaatg taggcctttg agttaagcac ctaccactcc
    19261 acgtcatacc ccaatccatc cagcgttcgg aggatcgtct cgaacgtagc ccctccttcg
    19321 tgattgagca atcctcggac gttctcaagg aataaaagct taggtcggag aagagtggtg
    19381 aaccgagcga tttcaaagaa taaagtacct cgagtatcgg aaaatctttg ccgctttccc
    19441 gcaattgaaa aagcttggca cggaaatccg ccgcaaagga tgtccactgg tccgagggat
    19501 tgaataaact catctgatac acttgtgatg tcatgcattt ccacctcctt acttgtatca
    19561 tgaattgctt tataactacg tctagcaaat ttatcaattt cacaaaaacc gatacaatga
    19621 tggctcgcct gttccatacc taaacgaaag ccaccaatac cggcgaataa atctaaaaat
    19681 ctcatacatt acctcattca ttgcaaagaa aaaagccaag aacattttgc tcttggctca
    19741 tacattatta atttgtccta ctatttttta tttaatccaa ctaatttccg ttgtttttca
    19801 ttggcctgat cggctacata tttaatcacc gttttgaact cttttgcagt agcattttcc
    19861 gataaatccg caatgctctt attgtatttc tttacaattt tcgttacttg ttctttctca
    19921 gtcatgtaat tgaacctcca aatctttcct actataccct agtggtaaaa ataattctag
    19981 attatccgta gcataatgaa cttcggaaat attattcgta taatctttta ttatgatatg
    20041 aagcaataca tctggatgtt tactctttac ttttgcaatg ttctctcttg atttgaaaaa
    20101 agcgttaata aatgcctttt tcggcacatg acgtccctct gcaatttcac gttcttgtgt
    20161 aaatttccaa gcgattttag ggtcttgata aacatagaaa atcgtcactc gaaaatgttt
    20221 tttcaacgcc cgcgagatat ttttttcagc acttaatatt gcaaaagttg catccagaat
    20281 aaaagaatag ccatgatcta agacatatct aaatgcctgc tctaccaacc aagaagatgc
    20341 tttttgaaag agactagagt tttttccatt ataatcagga aactgggtgc gaaagtaatc
    20401 cgcatcaata atcacatggt tatcataatt ctcagctaat ccaatggcaa cctctgattt
    20461 tccagcccca ggactccctg ccataaaaac tgcgtctcgt ccaacttcgg gggatttccc
    20521 tttgataata taagaaataa acttatcctt atttgctttt gcatactcta aataagaatc
    20581 attactttcc atattaatta tcccctttct tcctactata taaaagtatt atacgttact
    20641 tgaagtataa agtcagtatt gaagatcact caatcattca tcttttctaa tttactaatt
    20701 gaatctaaga aatcataccc ttttggtaca agtggcgtat aaaactcact aatcacactg
    20761 ccacctgtat ccacataacc tcgacctttg atgtgcttca taaagaagtt tttttcgacc
    20821 tccccaaaca tcatggaata tccgagttca ctcatacgac ccaaagccac gcgaaagttg
    20881 aattgatcgc ggattccgtc tcctaaatat ttcgcatccg gcctttggca agccaaaatc
    20941 agaaagaaac cagattgacg acccaacatc acgatttgtt tgagcttatt caaaatcacc
    21001 gcactttctt tcgtcgttaa catctccata taagccacgt attcatcaaa gattaaaaag
    21061 ttgggtggaa gtccaagata ggcataattc tctcctgttt tgtagttaga catttctttc
    21121 attgccttgc tacgagccat catacgttca tagaaatctt ccacacaagc agaaatttct
    21181 tccttttgtg aataaacatg aggcatcacc gtacctaaat cagctaaatc tgcatttttg
    21241 ggatcgagga taaacaattc tgcatctgac ttcaatagtg cttcgataat ggtgaggagg
    21301 aaataggtct tccctccacc cgtgccacca gcgattaaca tatggggtaa agaatcataa
    21361 gcccatactt gatttttcat caatcgcaga gtcccatttt ctgccactac ttcgtctatc
    21421 ccaattcgat tggcgatcat atcatacagt aaagtatact ccacatagga gtctttgagt
    21481 tccttttcta ctaactcaca atacagtccg ctttctaact tcttttccaa ttttaaaagc
    21541 tgatcttggt atttcccaag tgaaatttga acgcgaatag acagtaatcc atctttcatt
    21601 cgataacaga ttttggggaa gtaactgata gtctctctgg ttcggctact attcaaatct
    21661 ttgaaaaagc cttcactttg agtttgtttc acttcatacc aatggttctc tagaatcatc
    21721 cgagctagct tttgtcgatg ccacaagctt cgccaatgat caagaaaaaa gtgaatataa
    21781 agcaatatca tagctcctga aaaagcgaga cttccaagca gacatactaa atcttttata
    21841 gaccaaacga tttcaatagt agaaagtgat agcttagaaa tatcaaattg tctaaactct
    21901 tgaaaataaa agacgctcat ccaaccgatg aacagagcga gtagccagcc aagaacaaag
    21961 tgataaacca agtgctgatc tcctgcgcgt atgcgatgtc ctttgtacat tttgatggtt
    22021 tccacctcca atcgaaaaag cccaatagga tttcattagg caatttcaaa aagttatgct
    22081 agtcatttac tgctacgagg aggggagttg tttcgtttca accaattgat ttaaaaagcc
    22141 ttcaatagca tccggcatct ccacaccaat ttcttccaaa atggattctg cctgccgaca
    22201 taagttttca tttacttcga caactataaa gaaagactct tcttctccta accagtctag
    22261 tacatcagag tcttcttcac tgtcgctatt ttctccaagt aaaccttgat gtataatcag
    22321 aaacattttt tcatcagagg ttaatgcttt aacgagttgt tcctcagtca gctcgccttt
    22381 tccatattga tcgagacaat ctaaaatatg ttcaacatta atttccataa tcttcaccta
    22441 tttctttctt tcattttggt tcgtaggatt tccttgattc ggctgccgct tcaagactaa
    22501 atcatccgcc ttaatgaacc aattaacggt agttccttct cgaaaaacat aattggcaac
    22561 ggtatccacg attggattga ccaactctac ctctgcatca taagcaaact cgcgtaaggg
    22621 tacattagct gggatactaa cttgaatcat tcgcccttgt gcagaggatt ttaaattata
    22681 ggttcgttct tttacctcat taccaattgt tccatcctca ttttgcttcc gtacttctcg
    22741 gcgtaatgcg gaaaaacgca acttcccaaa tgttaaacct gaatctacga caataccttc
    22801 tgttaatctc atcttctttt cctcctatgc tttcactaaa tcatcaacat acaacacgta
    22861 atcagtaaac ccacgattct caatctcata gcctttggca accaaatggg gattcacggc
    22921 ttttagcggt tggtcttgat caaaatcctt ttcaccggct tccgcagcta ctaccacctc
    22981 aatatcatcg gcccgttgct tactggaata caaatgataa ctgcgaccaa tcaccgtagt
    23041 attccgtcca tatccttctg ttataatttc tccttcaccg gcataactta actttccaaa
    23101 ggtttcagcc atatctggca cgacaaattt caattccatc ttcttttcct cttttcattg
    23161 ttagttttgg atacaaaaat agcgcgacta cttttttagg aggcggtcgc gcacgttggt
    23221 tcttaggatt tacttgtatc atctcatcac cttagttccg gcgattccag aaataatagc
    23281 ccgccgtcgc aaaaatcaaa gtaaaaccaa tgaacagtaa aacattggaa tttttctccc
    23341 cggtttgagg gaaggtcttc actgtgggac tagattcttg agagccactt ggaacagttg
    23401 gtgtttccgg ttgtttcggc gtacttagta tggttggaac ttcttttggt gttaaggttt
    23461 gagatttttc tttcaaatct tcattgtgtt ttccattgat attcccatct ttatcgtaat
    23521 tgatttccgc aaaagtaaac ttggttcctt ctggatattt tccggtatct acttttttcg
    23581 caagcacagt tttggtgaat tctttatcat ttacttcata atcaatcttg ccagatttcc
    23641 aaatttcttt gtttgtacca tccggtaata aagcatacag aattgtttcg aaagcttctt
    23701 ttgagccatc cagtacatca tgagtaatcg acacatcatc aaacatatcc accacatcac
    23761 catgagtaaa ggtctgagaa ccgttttcta ggtgggcctt tgtttggata gaaacatggt
    23821 gttccactgt acaactgaca gtttgggctt ggttattcaa gctaacatcc ttcgctactg
    23881 gcttatcgcc tgcttcaaaa gcttccttat tttcatacac atagttgaat aagacaatag
    23941 ttttatttaa aacttgttcg gcggttaatt tatgctgaat tttccaagtt cctgttttct
    24001 ctttgttcgc caacgttgcg cttgtttctg caatcaccac tggtttggct ttttcatctt
    24061 tttcttgggc agcttttgtc gcatccacat caatggcttg cgctaccaca taccagtctt
    24121 ctttgatttc atgtagattg taactcaatt tatcaactag ctcggtagaa tccagagaag
    24181 ttaattcttt gtttccgtct ttccaagtcg caagagaagt taaactatct tcttcctcag
    24241 gcaaatcata gagcattaaa cggttcaaac taacagaaaa tgctttgttc gtcagcttct
    24301 cgtaaggaac ggtcaccgtt ttaattggtt gtttttggtc ttgttcggta atggtaaaga
    24361 catactcact cttcacaaag tcttctttgt tttccttaaa agtagaacgg atttctaatg
    24421 gcgtaatctt ttgaaaacct tctggggctt ctacttcttc aagtaaataa tccccataag
    24481 gaagattttc aaacttgcca taaccatcaa accctaattg ctcgttataa gctgtggtcg
    24541 cttcatcttc ggcacctgtg atttcattgg tcccttccaa tggcgacact ttaaaggtca
    24601 gatcattgaa gccagtttcg gtagtgcccg cagctgatcc agcaaattta aagaaatcaa
    24661 agccaaagcg aatcacttgt tcttttgccg taacatttcg ggtaatcacg gcattttttt
    24721 cgctatcatc cacctttttg atggatacag gatactttgt ttcatcaagg ctatatcctt
    24781 cgggcgcttt tgtttcttgg caataatact cgttgatagc taaatgttta acggcaactt
    24841 ggttcttttc gtctaaagtc aaagtgaccg tttcatcaga acctttcgtt cccttcgcta
    24901 actctggttt aaaagcctca ctccatttaa cagcttttcc atccttagca gtaaacaagg
    24961 tgtattcagc accctcaaag actgccttac cttgtgcttt atccccagta tctttgtctt
    25021 ctttcgtcaa agtggtttcg ccagtgactt cttgattttg cccttttacg ttactggtaa
    25081 caggggctac ggtttgattg gcatacttca attcgacttt tacaggtttg aacgtattca
    25141 cgaacccatg actagcttta gtttcggtca cataataggt tcccaattcc aacgcattag
    25201 caatctcttt tggtgtttcc gcatgaccgt tttcatccgt ggtcatttct tgaacgattt
    25261 caccggttgg actgtctttg cgaatggcaa acgtattacc agctaaggaa taattgtcat
    25321 tccaaagatc actccctgtt tcaactccgc ttttatccag aatgatttgc cctttttccc
    25381 gtgcgttttt tgaagtcacg taaatggttt cgcctgcttt aatggtggta gtcatggggg
    25441 tggtatcaat cgtataaggc gccggtactg atttttcggt aatggttact tttgtaccat
    25501 gaggaatctc atccaatgta gcaatgcctt ctttgtcagt agtcacgtct tttgcaggta
    25561 aagtttttcc aaagtctaaa tggaaaaccg ttcctggtac aacatcaccc gattctttat
    25621 cgactttttt gatctttaaa gaaccctccg tttccacatc aattttgatt gtgtaggtat
    25681 taggtttatc aatcgccccg gccattaaag tctgttggcc agccttttta taggcgacag
    25741 gtgttccggt accagcagat ttcttaagcg tcagcacacc actttctttt gaattggcat
    25801 ttggggtgat tatcaactga ttaccattca cacgataatc aatgtttgcg gcattttcca
    25861 caacctcatc gaactctgac aaattcagat tattcgtatc cgtcacagta gtcgattgcc
    25921 ctaatactgt tttagccgta ctattatgga aggttggttt cttctgataa tcgtcaatgg
    25981 cttgatttat ttttgcctca atggctgcga tatttacggc actaccatct gatcgtttta
    26041 ttgagtgaag ggtataccca ttaacttctt gccagatcat cttctgtgcc acaatatgag
    26101 tatctgcatc tgtacctact tttttccata aaacggaaac taatttggct ttatcggaca
    26161 tatctggtaa cgggtttttc tcatagccag gagtgaactc attgtaaatc gggactcctg
    26221 gttcaataca gaaaatatct tgtttaaagc cgttgtacac tgcataatgc ggaacagtga
    26281 cttctgacat tggacgacca tcactaaaag ttccttcaac agcatattga tgtgctaaat
    26341 ctagttctac ggttaccgtt tgtggatggg taatttcatc agcaaaagta atcgtaggag
    26401 caattgctcc aacaattgct tgaacagcca aactcaaaat agttaataca cataatttct
    26461 ttttttgttt tttatacatg aaatgttctc ctttcaaatt aagcacaaaa aaataagatg
    26521 ctcgcttgaa catctttgtt tataaagtct ttttaaaatt tttattgact gaatagaatg
    26581 gatttgagaa tgaatgagcc taaaaattta atctaaaata cgaaatatac tgcctttaaa
    26641 ttagaacaca ttttagaaca caaacatttc aaatttaatc aaatataata aaatatattt
    26701 acgggaaaac ctttataaat caaggttttt caaacttatt caatgatacg aagaaaaaac
    26761 caggtcttaa gaaagcccgt aaagcttcac agttctcaaa acgttaattg cgattatcgt
    26821 tcgagttcac gagttacgag aatcgtactg ggcgaagcaa tgctgagcgc agttacctta
    26881 atcctaactt ttctggcact ttccacaatt tggaaagtgc cttttttgtg tttttagcta
    26941 tgttaatagg taggattctt ggtctataat atcattcgat ctataaacaa acgggaaaaa
    27001 gataaagtat catcaaacaa tattatttat ccgcttctga ggttgtgaca gaagtggtca
    27061 gcttcaagaa ataagaagga atttacaaaa attgcttctc atgtttgata aatattgtga
    27121 cttgtgaccg aggagttggc aggagaacac cgtttattcg gaagaaggga ttgtgaaaag
    27181 tcttttgtca taacctctta tttggtagct aattgtaaat cccaaacggc atgtattatt
    27241 tttcataagt ccgtttttaa ggattaatgt tttcttttgc tttttatatg gtctggcttc
    27301 agcgagaagt tccaacgcat atacaaggac gacttatgtc aacgataaca acgattacca
    27361 tggtggccag tccacttgga ttagcactgt ttggtgcgct ctttgattgg aagacaacgc
    27421 aaccacgtct gcgagacagt ctaatttttc tgggagggag tcttgctata gttatcctta
    27481 taaccggtct ggtaaaatta ctcaagcttg atctgaaaga agcaaaaatt ttcagtgaag
    27541 aggaatacag agaaacgact ttggtcgaaa agaaagaatc aggaaactga tacaagcgta
    27601 taacaaactg cctgataata ggaaagacac gtattgtccg attgtttttt ttgacattca
    27661 attggaaaat ttacactttc tttatacatt tctggctgct ttctttatac ccgtcaacta
    27721 tacttcactc atcaataaga aagaaggata agtatggatt ttggaaatga aacggtcttg
    27781 aaagcaactg gtataaccaa aaaatacggt gctgccaaag cattagataa agtatcaatc
    27841 gaaatcaaac gtggaatgat ctatggattg attggggaaa atggagcggg taagtctacc
    27901 tttatgagaa ctattatggg tttgatctcg attgatgaag gttcgatcga attatttgga
    27961 acgacggatt tgcaggcagc aagacgacgt atgggtcaat cgattgaaac ccctgctctt
    28021 tatccagaat taactgcaag agataaccta cgtatccaag cagcgaacgg cggtgtgtcg
    28081 gatagagaaa tcgaagacct gctgaaaatg atgcgattgg aaaatacagg gaaaaagaaa
    28141 gcaaagaatt tctctttagg tatgcgccag cgtttagcta ttgcaaatgc actgatcacg
    28201 aatccagaat ttttgatttt ggatgaaccg acgaatggga tggatccagc agggatggca
    28261 gaaatgcgag aaatcatcca acgtttggta aaagaacggg gcatcaccgt cttgctttct
    28321 agccacttgc tagatgaatt gtcacagatc gccactcact atggtatttt gcatgaagga
    28381 cacttgatca aagaattatc gaaagaagaa ttggcacaag aatctcgtca atttataaaa
    28441 attgacactt cggcgacgga acaagcagta acggttctcg actctcttgg ttatagggat
    28501 tattttgtac aatctagccg tgttatccag ctttttgaag gaattgacca agtagcagcc
    28561 atcaatcaag cattggtaga agcaaaagtt cctgtggatg gtattcacct agttgggcaa
    28621 aaactggaag attatttctt acaattaact ggaggcaatc cacatgttta atctattaac
    28681 tgctgaaaaa ataaaagtat ggaagagccg taaaatgtgg gtctcactag gactgatggt
    28741 cgtacttccg cttttttatt cttggaacga atggtatatg catacaaaat acggcaatga
    28801 attaaaccaa gcaacggata cagtcatcaa cggtgcaaca gggattttaa tggtagaaaa
    28861 aatggctgca tggattcttc tggctttcgc agcatttgcg tgcttttata ttggggaaga
    28921 atttcaaaac ggtacgattc gcaatacgtt atctttagga cggaatcgaa tgacttatta
    28981 tgtgtcgaaa cttctcgtta cgcttttgat cacaacactt ggcgtggttt tgatcacggg
    29041 actagccatg cttgcttaca cacttgcttt cggatttggt gaagttgagg gaatcaaaaa
    29101 ctatggaaat tatgttctaa aagtatttcc tgtattgctt ctgctgacat tagggacatt
    29161 atctgttcca gtggctttga cattcatcac tagaagtacc agtgtcagtt tgcttctttc
    29221 ttttctttat atcatgggaa cggccttcat tcccggtgtt ttcgcaaaaa tcaaaggctt
    29281 ggaatttcta acggagtggt ttacggaaac gtggctcatg tatacggact ttgcgcaaca
    29341 agcaacttat tcgcaagcac cgaaaatgat tctggtcagt ctagtaacaa tcgtggtatc
    29401 atcagctcta ggaatgttta ttttccaaaa atctgacatt aagtaggtgg tcctgctgga
    29461 aactattttg attatcgaag acgatgaagc tgttcattcc ttactggagg aggttcttga
    29521 acagagatac aaacttcttg atgcttattc aggtacggaa ggaaaattat tgttaagcac
    29581 atatcctgtc gatttgatcc ttctagatct catgcttcca gggctttcag gtgaagcatt
    29641 gttagcagaa atccgacaga catcgaatgt tccgatcatc gttctttcag caaaaagcga
    29701 tcaaagagat aaagtttcct tgttggcagc aggagcggat gactatgtga cgaaaccatt
    29761 tgatatcgaa gaacttttgt tacgaatcgg catccaactt cgtcatcaga cttctgtcca
    29821 agtaaaagat ctgtcgcaac gcatctatta caaggaaatc ttactggaca aagagtcgag
    29881 ggatgtaaat gtgaatggtg tggctgtcca tttgacagga agagaattcg atctgctgaa
    29941 gcttttttta gaacatccga aaaaagtatt ttcgcgagca aatctttatg aaacagtttg
    30001 gcaagaacca tattttgata gtgagaaaac agtgaatatg catatttcca atcttcgagg
    30061 gaagctagca aaggcacatg ctgtgtatat tcatacggtg tggggagtgg ggtttagatt
    30121 tgattaaatg gctgttgctt ctcgttgtta tctttatggg aagttttatt ttcttcctgc
    30181 aaagggaaat caagagatta actaagaaaa tcgccactct ttctgaacga caagaatatg
    30241 gtggtcgtct ttcagtcgaa tttcgagaaa agaatctaat ggacctagta gatgcactga
    30301 atacatggtt ggatgaaaat gaacaaaaag gacaagcatt tcaagaaatg gaagaaaaca
    30361 tcagactatc gatcgtcggc ctttctcatg atctcagaac tcctttgact gctattaacg
    30421 gttatgtaca attactggaa caggtagagg atacggagaa acgggagaag tatttaacga
    30481 tcatcaaaca atctgttgca agattactca gtatgacgga tcagttttat gatcttgctc
    30541 gaatagagac gaagcaaaaa gaaatggatc tccatacgct ttctttttct aaagaggtgg
    30601 aagaagtatt tttctctttt tatgaacaat tttctgagaa aaatatcgag gtagcgttcg
    30661 ctgaacactc gataaataca actgtattag cagatccgct catgttgctg cgagtgctgc
    30721 aaaatatcat acagaattta ttgcgttatg cagatggaca agcaaaggtg acattttcaa
    30781 aagaggatcc attcattcga ttaacagtgg ccaatcctat caaaccagga acgcaaacct
    30841 ctgttgaaaa agtcttccag cgtttttata ccgaaaatac cagccgcaca aatacggaat
    30901 ctagcggaat cgggctttac ctgtcaaaaa aattggtgga aggaatggga ggagagatga
    30961 ccgctagatt agacgggggc atcttctcca tctccgtcaa gttgcggagg gtttaattat
    31021 acaagattag tgagaactag tagaaaaatg tattatctta tatatattta atagagaaaa
    31081 aagcctgtga tataaatatt gcaggctttt ttattgattt gaaataatca agaatattag
    31141 aaacttctac attaatcaag aactttcttc tgtatgaaaa tagagataat aattgtacac
    31201 ttaagctgta ttcaggaggt gaagataatg ggcatcgaat ttagcgaacg gcaaatgaaa
    31261 atactatact ttttgattga aaatgagcac acaacttaca aagaattatc agaacaatta
    31321 tatgtatcta cgaaaacaat tagcaaagat attttaattt tgaaagaaat aatccaaaaa
    31381 atgccagatg tacaaatgtt ggttcaacct aaaaaaggcg tttcgcttat tggaaaaaaa
    31441 gaaacacttc gaaatttatt atccagtcat aaaatcaata tcgcagactc tccggaggac
    31501 cgtctaactt atgtgtttgc taaattattg aatactaatg attctctaaa gattcaaaat
    31561 cttgctgacg aattatacgt gagcaggtcg acggtagaaa gcacaatgaa agatgttaga
    31621 aaaagactaa acaagcatgg gatgtcctta gtttcagatc gaaatggcgt gcgcatcaaa
    31681 gctgatgaaa aaagtaaaag aaggttgatg tcagaagttg tgaactatta ttggggtggc
    31741 tttacagcat ctaacagtaa aacagatctc ttagacttga atatcaattt cattggtgaa
    31801 gcgaataatt tcttaaataa ggacatattg aaaaaagtag ccgaattgct taatcgatac
    31861 atgaataaga cacaattgaa atttacagat tatgaatttc aaactttagc tatccacctt
    31921 gctattgcga tagaaaggat tgaaacaagc aatttcttat cacgtagtaa taattctgaa
    31981 gaagaaatct caaagaatac acttatttta atcaaaatga ttgaaaaagc attttcaatt
    32041 attgtgccaa tagatgaaca agcttatgtt gacattcaca ttacttcgat tgaaaaaaat
    32101 gctatgaatc acgacttaga tcaaacgaag ctttcctatt tagatatggg tgttgagcta
    32161 caagagttag taagaattaa attaaatgaa ttttatcctg atgaagaatt gatcaataat
    32221 ctagtattac atctcaatgc agcagttaaa agactcaaat tgggagtaaa tatctataac
    32281 ccttatacgg acaaaatcaa atattctttt aaaaggtcat ttatgataag tgtagattta
    32341 ttagaggaaa tcgaagagag attctgtata cattttaatg aagatgaaat cgcttacgta
    32401 acactccata ttcaatcact tttggatcgt tacaaacctg acaaaacgaa agtgatatta
    32461 gtttgtagta gcggatatgg aacatcaaaa ttattagagc aaagaataaa taacggtttt
    32521 gcagatatgg ttgagataga agatgtacta agcatcaatg aactacaaga tagaaaagtc
    32581 actgatgaac ttgttataag cacgattccg attgaaaata caaattttcc tgttattgtt
    32641 gttagtcctt tgatgttagg ggcagatgtc agaaaagtaa aacagtatct acagttaaat
    32701 gaacaaacag aagatgcttt tctaaattta ttagataaac gattttgctt ttttacccac
    32761 caaataaagg ataatcaagt gaacgtttta aatataatta ccaaaaaatt ggtaaaagaa
    32821 aattacgcga aagaagggat actagaggga gcaatcaaaa gagaaaaact atcttcaaca
    32881 gcgatgaatg attttgctat gccgcatatc gaagtagatt ttataaaaaa gccagtaatc
    32941 tgtgtctacg ttaatccaga aggaattgat tgggatggga cacttgttca ttctgttttc
    33001 ttttttgcac ttaatgaaaa agtaaaacct gaattgaata aaatttatga aacattcaat
    33061 gaaatcgctt cgaatcatga gttattagca aatttaaaaa cgagttcgtc gtttgaaaaa
    33121 ttagtttata tattgaaaga aggcttataa atgatcagtg aaaaaatcaa tataaataat
    33181 atagtgatag atttaaatgc atctgataaa aaaagtgtag tcgaaaaatt atcgaaattg
    33241 ttagagacta atgggtttgt gtctgaccac aaacaatttc aaaaagacgt atttgacaga
    33301 gaaaaccata tgaccacagg aattgggaaa aatatcgcca ttcctcatgg caaaagccta
    33361 gcagtaattg aatcaacagt ggctgtagcc aagttaaagc ggtctgttga atggggcgca
    33421 ttagatgatc aaccagttga tctcgtcttt ttgttagcaa taaaagacac tgataaaggt
    33481 actaatcatt tacgtatttt agcagaatta tctggaagat tgatggatga cgagtttgtt
    33541 aaaagaataa aagattcgag aaataaaaaa gaattggtaa aagctctatc attttaaaag
    33601 gaggaatttc atatgacaaa aatagtagca gtaacagcat gtatcgcagg gattgcccat
    33661 acttatatgg caaaagctaa tttagaaaag tttgcgaaga agaaagggtt agatattaaa
    33721 gtagaaacac agggggctat ggggatagaa aacaaacttt caatttcgga tgtttccgag
    33781 gcagatgttg tcatatttgc agtagataca aatgtttcag aacgaggtcg ctttgacggt
    33841 aaaccgattt taacagtagg gacctcagag gtagtaaaag atggtgaaga gacgattgat
    33901 gaagcaattg cattaattaa caaataaaaa atatatcgtg ggggagttac taatggaatt
    33961 caagaaacaa ggaagagaaa ttctaaatca tttccaaagt ggggtttcct atatgatccc
    34021 gcttgtcgta gcagcagggt tactaacttc tattgcagtc atttttggtg gcacaggtgt
    34081 ttgggaccag acggatacat tctggggagt cctgcgaatg attggtcaga cgggattaca
    34141 gtttatcgta ccgatgattt cagcctatat cgcatattca attgctgacc gtcccggtct
    34201 agcaccagct tttatcactg ggatgatgtg tcaaaatctt ggtatgggat ttattggagg
    34261 aatggttgct ggattagctt gtggctattt agctaatgca ttgaaaaaag taaaagtacc
    34321 gatgcaagtt attagtctaa aatcaattat gctgattcct tttgttacta cattagttgt
    34381 tggaatcata ttatggtatg tactagctac gcctattcaa gcaatgacaa caggactgac
    34441 taattggcta gaaggcatgt caggagcaaa tgctgctttg atgtcggcta ttttaggagg
    34501 aatgatggcg tttgatatgg gaggacctat caataaaatc gcatatgctt ttggggttgc
    34561 ttctttttca agcggaagct ttggacctag tacagccatg ttattaggaa ttgctattcc
    34621 gccatttgga atgtttttag caacaatttt aaacaaaaaa ctttatgatg aatcggaacg
    34681 agataatgca aagacagcta tgattatggg ccttgtgggg attactgaag ggacgatccc
    34741 gtttgccgtg aaagatcctt tacgagtaat accaagcatc gtggtgggca ctgcagtagc
    34801 atgtggtcta aatggatttt ttggtgttac tcaagaaaca atgctttcaa cctttatggc
    34861 aattcctttt gttacgaata tacctttata tttagtatca attgttattg ggggagtggt
    34921 tacagcgtta atggtcaatg cactaaaatc catcaagtat aaaaaagaac aacaagtaaa
    34981 ttcagtagaa gaaggaaagt aggagaatct gatatggtaa atgcacatat aattcaacac
    35041 acgcactggg acagagaatg gtattttaca aatgaagatg ccatcgtatt gtctgatcag
    35101 gtctttactg aagttctaga tgaattggaa aggaacccta aagtaaactt ttgtttagat
    35161 ggacaaactt caatcgtcga tgagtatttg gagatcaatc cgcaaaagct taaggttatc
    35221 caacgactga tgaaaacagg acaactattc gtgggaccgt ggtatgcaca aacagatgcc
    35281 ttattagtgg atgctgaatc aattttaaga aacttaatga ttggaatcag tgatacgatc
    35341 cttaaatatg gagatccaat gatggtaggg tatttgcctg acacgtttgg gttcaatgcg
    35401 aatttgccta cacttttgca tcaagtagaa atcgataatt tcatgtgttg gcgaggatta
    35461 aactttgaaa cgatggttcc atctccttat tttatctgga aaggattggg agataaattt
    35521 gtttatgcca tgaattttcc atttggttat atgactggaa tgatgacctt agacgcaatt
    35581 aaagatattc ctgaatttgc aaaagaaaag ttggatgaag cagttgaaat gcttcatatg
    35641 caaggaaaaa acaaagatat tttagtacct tcgggtgtag atcaaaagtc aatgatttta
    35701 aattttgatc aagtcgtaga gaagttgaat aaatacagta aattccacaa tattatttca
    35761 gattatcctt cttatgtaga agttatcaga aacaaaaaag aattacctga atataaaggt
    35821 gaacttcgtc agccggttta ctctcgtgtt catcgttcta ttggctcagt aagaacaaaa
    35881 atgaaaatag aaaattttaa attggaacaa aaaattctaa aacgagttga gccgctcatg
    35941 ctgattgcag aaagaaatgg tatccatatc agtaaaggcc tacttcgtcg gttatggaaa
    36001 aagattttgc aaaaccaggc tcatgatagt attggtggct gtgtttcaga taatgttgcg
    36061 gaagatattt tccatcgaat caaagaagct aatgagatag ctgacggaat cgaaaacctt
    36121 attgtaaaac ggatggctga tgcattaaag ctaaaatcga atgaagtcct tgttttcaac
    36181 acaacgactg agaattatag tggatcgaaa atcgtgcatg tagtagctga ttcaaagaaa
    36241 atacacttca aaggtagtcc ctataatatc attgaaaaag aagtttattt tccagaacgg
    36301 aaaaatgctt atcggatgat tgctacaggt ttcgaatatt ttgatgaacc agcttattat
    36361 gaacttgaca taaaaataaa tgtaaatgtg ccagcactag gatacactgt tgttgaattt
    36421 gagaaaaata atgaaaaatt agaaacagca atcgagatag acaaaactga gataagtaac
    36481 aataaatata aactttcttt taaagatggg caattgaatt tgatagttgg agatagacaa
    36541 tatcttgatt tcgttcattt gatcgattca gcaaatgatg gcgatacgta tgattattca
    36601 ccattagaag gagatacaga actatctcta aaattagaaa cagctaaagt atataaagat
    36661 tctttacaag aaacattagt tgtttacgga aaagctcaac ttcctaaaga tttaaaagat
    36721 cgtttatctg aaaaacccga aatggaagaa atatcttatg aaatatcgtt cagtctggga
    36781 gaaagtcaaa tcgttgaagg cacactcaaa attcataata ctgtttactc gcatcgtatg
    36841 cgcttagcaa ttaatgtttt agataaatct gaaaaatcaa ttgcacaaat tcaaaacgga
    36901 tttattgaaa atacccctga tgtcattcct ccgaattggt cagaaactat ggtagagaaa
    36961 cctgttaatt tagaaatatt cgataagagt gtctctgtgg cagaaaatga tcattatttt
    37021 acgttttatg cagatggttt aaaagagtat gaacgtatag aaaatcaatt aatcataacc
    37081 ttattttcaa caacagggga acttggaaaa cctaatcttg cttggcgacc tgggcgagct
    37141 tcaggagata cgacaaatga agggcatgtc atgatggaaa caccgctagc tcaagagata
    37201 ggtgagtata aagtcacttt tggatttaat gtagaagaag gaagatttaa tgagttcaaa
    37261 gtggcaaaac aggcagaaaa acggttagaa caatctattt cttatcaaaa gcagaaactg
    37321 aatgtattta ttcacaggtt agataacaag atttggccat tacaaaatga acttgatgtt
    37381 cctcgtaaat tttcattgat tgaattacct gaagacttaa ttgtatcatc gatttatcct
    37441 tcttattttc atgaacaagg atttgttctt cgcttagcta atccaacaga acaagaaaaa
    37501 atagtacctg aggctatcct ttcccttggg acagttgtca atgcactgga gaataaacaa
    37561 gaattaacaa caattcctcc ctatgattat ttgtcaattt tgataaatga gagataaata
    37621 tgtaatatac aagaaaaaaa caccttttat tttgaaaggt gtttttttac cctcttttca
    37681 aatagcgcaa cagtgctata ctaaagctaa caaataaagg aacggagagg gatatgggtc
    37741 aaagagaatt tatcgtttta attattattg cagctgttat tttattagtt gctttagaca
    37801 ttttttcacg tttgaaatta aaggaaacag ttcgtagcaa atgggaaaaa attccttatc
    37861 aaccgagatt tgataaagaa gaaagcttga aggaagcatg gctcacggag aagaagttcc
    37921 gttcatggga tagcgaaatc gatgatctta cttggtatga cctggatatg ttcgaagtat
    37981 tcgaaggaat caacagtacc tattctagtg taggttcaga agcgttgtat caacgtttac
    38041 gcagctttga ttttggtgaa gatcagcagt tagaaaaact gattgctttt tatcaggaaa
    38101 atcctcaact gagagaaaaa atccaatatc aattcgctcg tttaggaaaa aaagatcata
    38161 attttgcaaa acaatatcta gcggatggaa aaagtaaaaa gattggacgc gtaggtttat
    38221 ttatttttct tggcttgctt ccgctggtgg gtttgggttt gcttctttta ggacaagtac
    38281 taggaatcta tttggcaatc ggtgggttag tattcaatac ggtctattat ctagtgaaga
    38341 aacaaacact agaaacagag ctgaatagca tgagttattt ggtttcaacg atttcagccg
    38401 ccaatcagct agcaaaactg ccgacaccac ttcaagaaga gctgaaacag caaactaaac
    38461 cattaagaga catccctaaa ttaggttttt catttcgtat gaagagtggg agtgaagcag
    38521 agttgatttt tgaatatgtc aacatcatgt tcatgctgcc atttatctcc tatcatttcg
    38581 ttctcactaa attagaaaag cattcaactg aagccatcaa gctgtgggaa ttgctgggag
    38641 aattagaagt agccgctgca atattgaatt accgtactta tatgcccatc acttgccgac
    38701 cagagtttac tgagggcggt gtgacagcga aagatatcta tcatccattg ttaaaagaag
    38761 cggtagtgaa tccggtcaac tggcagaaga atacattagt cacgggttcc aatgcatcag
    38821 gaaaatctac gtatgtcaaa agtatagcca tcagctgtat tctagcacag acgatccaaa
    38881 cagcgattgc agaacagttt accctgcaac ctggacatgt gttgacttcc atggcaatcg
    38941 aagatgattt atttgaaggc gacagttatt ttgtttcaga aatcaaatcg atcaaacgtt
    39001 tgttggacca agtggcttcc aaagaacgct gctactgctt cgtagatgag attttaaaag
    39061 ggacaaatac gatcgaacgg attgcttctt ccgcttcagt cgttcggtgg cttgcggagt
    39121 atccatcttt agcatttgtc gccacacatg atatcgaatt gacagagatt ttaaagaatt
    39181 attgtgagaa tgtccacttt gaagaacaag taacagaagg aaaaggaatc agttttgatt
    39241 tcaaattaaa agaaggacct tcaaggaccc gaaatgcgat tgccttgctg aaagttttgg
    39301 attacccaga gactttagta gaaaccgcaa aaacggaagc cgcgttattt gatgaaaagc
    39361 ggcagtggca tgtattggga taggcgaagt gatcgaaagt atttattgga tcaaaaatct
    39421 tggcttttgc ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt
    39481 ttgtctcacg ctccttttgt tgcacatatg aatagtaatc ctttatcttc gatttgtttt
    39541 ttctgaaaag gttgtctgac ttcgtccgat tgatgtcttt cattaggaga ttttcctgag
    39601 cccagcaacc tgatttttcg ctggaaccaa gttgtttttt catctgctgt attccatgat
    39661 acaataaaga atcatgtcta taaggagata ggaaataaat ggcaaatgcg cttgaaataa
    39721 ataatttgaa aaaggtttat ggaagtgggg tcgaagcatt gcgaggaatc gatctttccg
    39781 tgaaagaagg agatttctat gcgctattag gaccaaatgg agctgggaag tctacaacga
    39841 tcggaatcat tacttcttta gtcaataaaa catctggaaa agtgaatgtc ttcggttatg
    39901 atctggatac ggatctagtc cgagcaaagc aacagattgg attagtcccg caagagttca
    39961 atttcaatcc ctttgaaacg gtccagcaaa tcgtcgtgaa tcaagcaggc tactacggtg
    40021 tctctcgcaa agaagcgatg aaacgcagcg aaaaatattt gaagcagtcc aatctttggg
    40081 aaaagagaaa tgtccgtgca cgtatgcttt ccgggggaat gaagcgaaga ctgatgattg
    40141 ctagggcatt gatgcatgaa ccacgtttgc tgatcttgga tgaaccaaca gcaggagtcg
    40201 atattgaatt gagacgcgag atgtgggagt ttctccgaga attgaacgaa aatggtacaa
    40261 cgatcatttt gacgacgcat tatttagaag aagctgaaat gctttgtcgc aacatcggga
    40321 tcatccaatc aggtgaactg atcgagaata ctagtatgaa agagcttcta tccaaactgc
    40381 aatttgaaac atttatcttt gatttagcgc catatgagac caaacctgtg attgaaggat
    40441 acgcttgtac atttgaagat gaacagacaa tcgctgtcga agttgaacga aatcaagggg
    40501 tgaacggaat cttcgatcag ctgacaagac aaggaatcaa agttctttct atgaggaata
    40561 agtcgaatcg tttagaagaa ctgtttttga aaatcacaga agaaaataat catacggagg
    40621 agaaaaatgt ttaatcttta ttttactgca ttaaaaagtt tagctatcaa ggaaacgaac
    40681 cgctatttgc gtatctgggt ccaaacactg gttccgcctg tgatcacgac atccttgtac
    40741 tttgttattt tcggaaaaat gatcggtggt cggatcggtg acatgggtgg tttttcatat
    40801 atggaattca tcgtgcctgg tttgattatg atgtctgcta tcactagttc gtatgccaac
    40861 gtttcttcgt ccttcttctc tcaaaaattc cagaaaaaca tcgaagaaat cttggtagcg
    40921 cctgttccta cgcatgtgat catttggggc tttgtgattg gcggagtggg tcgcagtatt
    40981 ctcgtcggct ctctcgtaac gatcatttcc ttgttctttg taccacttca tgtgtattca
    41041 tggttcatgg tcatcattac attattgatg acggcaatct tgttttcgtt agcaggactg
    41101 ctcaacggga tttttgctca gtcttatgac gatgtatcga tcgtgcctac atttgtcttg
    41161 cagccgttaa cttatctagg tggcgttttc tatgccatct ctatgttgcc gccattttgg
    41221 caagctgttt cgaaagtcaa tccgatcgtt tatatgatct tcggtttccg ttacggattc
    41281 cttggaacaa tcgatgtgcc agtagcaaca tcgatgatcg tacttgtgct attcatcgtt
    41341 gttttgtacg ccgttacttg gtatttgatc aataaaggaa gaggattgag aagttagaaa
    41401 agaggttgtg aaatcagcgt tttgacctga gtattagaga aacaaacggg aaaatagttc
    41461 ctcatatttt gtcgtttgtt gagctaatac cgagggtcag ctgattgaac accgtttagt
    41521 ggagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc
    41581 tcctcctatt tttcgtttgg ttggacttat taccgcagat gcaagctttt gaacaccatt
    41641 tattccggaa aaggaattgt gataagattt ttgtcacagt ctttttttgc tcaatcagaa
    41701 acatctttta agctgttcac tttgcttctt tttttgtttc ctagtataat gacaatgttt
    41761 ttgagaaaga ttgcgacttc actgcttgat agaatggaga gtaacaaata aatgactact
    41821 aatttttgga aagagctgcc aaagcctttt tttgttttag caccaatgga agatgtaaca
    41881 gatgttgtat ttcgccatgt cgtaaagcat gcagcagcac cggatgtctt ctttactgag
    41941 tttacaaact cagacagttt ctgtcatcca gatggaaaag acagtgtacg aggaagattg
    42001 acctttacag aagatgaaca gccgatggtt gcccatattt ggggagacaa gccagaattt
    42061 tttagagaaa tgagcattgc tatggcagaa atgggcttcc aaggaatcga tatcaacatg
    42121 ggctgcccag tccctaatgt agcagaacgt ggaaaaggca gtggcttgat ccttcgccca
    42181 gaagtagcgg cacaattgat tgaagcggct aaagctggcg gtttgcctgt cagcgtcaag
    42241 acgcgtatcg gctatacgga aatgtctgag atggaagaat ggatcagtca cctacttcat
    42301 caagatatag ccaatctttc tgtccatttg cgtacgcgta aagagatgag taaagtagat
    42361 gcacattggg aagtcatccc taaaatcatt gctttacgtg accaaatcgc tccgcaaaca
    42421 ctgatcacga tcaacggaga tatcccagat cgccagacag gagaagaatt ggccgaaaaa
    42481 tatggcgtag atggcatcat gattggccgg gggattttca agaatcctta tgcatttgaa
    42541 aaagaaccga gggaacatac agaaaaagaa ctgatcggac tacttcgtct gcaactagat
    42601 ctgcaagatc attattcaga aatcatcccg cgttccatca caggactaca tcgtttcttt
    42661 aaaatctata tcaaaggatt tccaggagca aatgacttgc gagttcagtt gatgaatacg
    42721 aaatctactg atgaagtacg agagatctta gacacctttg aaaaagaaca tccaacgctt
    42781 ttcagtgaac aatgatttct tgcttgaaca ccgtgaatta gaggtcgtga aaaagctgtc
    42841 ctgcatcaag taataagaac agccaaacaa aaatagctcc tcatattttg tcgttttttg
    42901 agcgaatacc gagggtcagt tgattgaaca ccgtttaaaa gaggttgtga caaaagtctt
    42961 gtcgcaacct cttttttaac caaaaaagtg caaatagttt actttaagta agagaataat
    43021 tatgatgaat aaaagtggaa caaatagcat actatatata ttgaaatgaa aactgtatgg
    43081 atgctccaac ttgatgaagc taaggaggaa ttcgtatgtc agaaaaggaa ttaatgaagg
    43141 tagtcggttt ttatgaaggg ttgtcgatcg atgatccaaa aagctttatt gatgaaaaag
    43201 aacagatacc tgtacccagt tcacgggatc ttctggtaaa agtcaacgca gtatctgtta
    43261 atcctgtcga tacgaaactt cgccaagata acgggataag aaatgcttta cgtatcttag
    43321 ggtttgatgg agtcgggaaa gttgttgctg taggtgatga agtgcaaaaa ttttctgtcg
    43381 gcgatcgcgt tttttacgca ggtacaacga caagagcagg aagcaatcaa gaataccaac
    43441 tggttgacga acgtattgtc gctcttgcac caaaaaatct ttcagatgaa gaagccgcag
    43501 ccttgccgtt gacctcattg accgcttatg aattgttatt tgaaaaattt ggtctgactc
    43561 cagaagaaaa tgccaaccgc gggaaaaaaa tcttagtgat caacggagct gggggcgtcg
    43621 gttcgatttt gaatcaacta gcgcattggg ctggcctaga agtctacgcg actgcaagtc
    43681 cgaagaattt tgaatggctg aaaaaaacag gtgtagacta tccaatcgat tatcatcacg
    43741 acttaagtaa aagttttcgt gaattaggaa tcgatcaagt agagtatgtc gctgtattat
    43801 tcgatattac ccgctacttt gatcaaatca aacaccttgt ccgaccattt ggacatgtgg
    43861 gaacgatcgt agggatcaaa gagccgttag atataggtgt ttggaaaaac caatctatca
    43921 gcttcgactg ggagtatatg ttcgctaaaa cagactataa ctatgaaatc gaaacgcaag
    43981 gagaagcttt agcgcacatt gctgctttag caaatgaagg gattatccgc agtacattga
    44041 gcaaggtcta ttcagatggg atgaatgcga agaacttgaa acaagcaacc aaagacgtag
    44101 aaacaggaca tatattagga aaagtcgttg ttagtggtcc gttcaacgga taagcaagaa
    44161 tacgccgatt tttttcttat ttgactttga taaaaacaag agcgcatgac agtaatttgc
    44221 cataagctct tgttttttta tttataataa gcgaatcaac gaaagggaaa gaattcctac
    44281 caagacgatc accaataagc tttttgaaag aatagcagct aaaagtgtag gaactgaagc
    44341 caaagcattt tcgagattga tctgcggcag atgtccgata ttttgggtaa atagactgct
    44401 gaaccataaa gcagacataa tgacgatcgg gacaaaactt aaatactcaa gcaacggttg
    44461 cggcaaatcg aattttttca gtaagacaaa tggtaggatc ctagaaagcc aagttgctaa
    44521 agtacaacct aaaatagtca gcaatgtaaa ctcaaaagaa ggcatgtttg atccacaccc
    44581 ccaatccgca cccaatcagt gttacaacta aaatcactaa gttacttggg ataaaaatca
    44641 gtccgacata taccagaatc aaagtgagac caatcaggat caactgtaat cgtttagagg
    44701 tgtttctatc agaaatgact tgaagataaa gtaacccgat aaacatagcg acgatggcga
    44761 aatccaaacc aaactttttc ggatcagtaa taaaattccc taaaagtgcg ccaacaagag
    44821 aagaacccac ccacgtcaaa taagaaatcc aattagctgt attcatccaa cgaaagttta
    44881 acttttgtcc agtatagttc aatttattca ttcccagtgc gaaactttca tctgtcagca
    44941 atgtgccaat cagcagattt tgaagcagcc gatttttctt gaaataagga gcaatcgtca
    45001 tactcatcag aatcattcgg gaattgacta gaaaggtcga gagaacgatg gagagcagtg
    45061 gactatggct agcaagcatg ctgaccgtga taaattgggc agaaccagca tagatcagca
    45121 gggacatcaa cgtgacgacc agcggatgaa acccggcagc tttcccaacg ataccaaatg
    45181 caagtccaat accgatataa ccaaaaacgg taggtaacgt gtctttgatc gcagttttta
    45241 tatctaactt ttcatccaat aacaaaactc ctttagcacg acataaattt tcataattgt
    45301 cgtcaaactt gtgaatattc taaattttta atgaagactt gtcaacttca attttaaaca
    45361 aataaaaaag actggacgta gtttaaaaaa atcttctcaa gttagatttc cagacctaac
    45421 ttctgaggat cactacattt gtcacagtct cttatactga caagtataaa tgaagtatga
    45481 agaaaaagta tttgttattg gtatttctca ttattcttgt tttctaatgt gttcacttca
    45541 agtggaattt tagaatatta ttcaactaac aagtctacat cgtatctaaa tccatttgta
    45601 tcacttaatt gcttgtaaaa ttcgccactt tttttaattg ttcttttttg agtagtaagg
    45661 tcgagtgaaa ccagcccata tcggttttta taggcgttga tccatgacca gcaatcaata
    45721 aatgtccaaa gatgataccc cttaatgtta catccttcat ttattgcctt ccataagtaa
    45781 gcaagatgat ctttgatata ctcaatccga taatcatcaa ttatttgccc tgtatcatcc
    45841 atgaaacgtt cttcatccgc aacgcccatt ccgttctccg agataaatgc ttcgatgttt
    45901 ccataatggt tcttgatatc catgcacaaa tcataaattc ctttttcgta aatttcccat
    45961 ccccggtatt cgttcattcg tcgtccaggc atgatatgtg gttcgaaaaa ccactctggt
    46021 aaaaatggac tagatgtatc caccatagaa gaacgagatt tgacgcgacg tggctcgtaa
    46081 tagtttagtc caagaatttg agcggtattt tcgtagatac atacaagatc ttctttcgta
    46141 taatcttcag gaagctgatt atattcttgt aataacagga taagcttttt agaaaaattc
    46201 cctttaacca taggatctaa aaagctccgc gtataaaata aatcagcgat ttctgctgct
    46261 ttcacatcag caggattttg actacgagga aaagtaggac tcaaatttag aataacgcct
    46321 attttactag gaagagctag ttctttaaat acttgtattg cttttgcgtg tgcaagtata
    46381 gtgaagaatg ctgcttgcgc tccgcgttta aagtcaatga ggtttggata atgaaaatct
    46441 tcaagataag tacctaaaat aggcccaagt ggttcattga aggtaaacca atgacaaact
    46501 aagtcaccaa attctttaaa acaagtttca gcgtaatgaa cataagcatc aactacttca
    46561 cgtgattccc atcctccttt atcttgaaga gtagcaggca tgtcaaaatg atataagttg
    46621 gcaaatactt tgattccgtt attattcatt tcggaaaata attttcgata gaaagaaatg
    46681 gctttgggat ttacttttcc ggtaccgcca tttggaaaca ttcgtgccca agaaatagaa
    46741 acgcgaaagg agttatgccc agtttttttc attagttcta tatcttcttt atattgatgg
    46801 atgtgatcgg ttgtaataga ggaatcgatt cgttgataaa accggtaagg ttgttcttta
    46861 aaccagcgat cccagatagt ctctgctttt cctgtttctc cttttccttc tgcttgctca
    46921 gctgaccaag cggaacccca ccaaaaatca tctggaaaag ttagcttttt catagttgtc
    46981 ataatttttt tacctcctgt ttccacttaa tgataacacc gcttacaact taaagtaaat
    47041 agttttgttt aaatatatat atctttttta ttgtgtgtat aattttgatg tggattttat
    47101 cggtttattt atgttaaagt atagatgaag atatgttttt gattggaggg tcaccattga
    47161 aaaaaattgc gaagtatatg gaaatttact tagatataaa gaaaaaaatt gtaaatggag
    47221 agtataaaat tggtgagaaa cttcctacag gagatgaatt ggcagttatt tatcatacaa
    47281 gcaagttaac agtaaagaaa ggcgtagact tacttgtttc cgaaggagtt ttgcgaagta
    47341 gaagtgggta tgggactgaa gtcgtcagaa caccaattga taactcacga gttcttggtc
    47401 caaacgaagg tttgtttagc gtggttggtg aagaacacgt agattcagaa atccatactt
    47461 tctcaattga acttccatcg aaaaaagtag ctgataaatt aagtatcagt gtaaaagact
    47521 atgtatataa tattgtacga agccgtttta ttgattgtca accatactcg attgagcaaa
    47581 cattcatgcc attagcagtc ataccaggct tggaaccaaa acatttaaag aaatctgtat
    47641 atgcttatat cacaaatgaa ctgggtctgg aaataaagac ttcgcacata cgtatcaaag
    47701 gagatactgc gaatgaattt gacgcaaaaa tattaaaaat agatccaggt gaatttatga
    47761 ttgagataga caaagttgtt gctctatcgt ctggtaaacc tttcgagtat tcggtatcga
    47821 gacatttata tcaagacttt gtttttgaag ctgtttttgt tgaaaaataa gctcgggaaa
    47881 attatttttt cagagcttat ttttttatac taaaaaagat atatatattt aattaaaagt
    47941 atttactttt tttgataagt gatgtattat ttgggtggaa aaagtaatcg attacaataa
    48001 gcataaagga gaaaaaatat gggcaaaagt agcaggaagg acattgtatt tgaaaaattt
    48061 ggtatagttg cgactaagtt ggggaatcag atccatttaa gaactttgcg agatgcgttt
    48121 gctacattta tgccatttat gatgttagca ggatttgtga cacttattaa ttcagtgatc
    48181 ttagaaccaa caggattcat gggggaaatt gtcgatacta gtacattgac cacgttacaa
    48241 caaattggta cttcaattgc caatggtaca ttaagcataa ctacattatt agttgtagca
    48301 gcagtatctt atcatatgtg tgcaagcaga aattatacaa atcatattgc agcggtatta
    48361 gttgctattt cgacatttgt tgtattaaca cctatgcaga tgatgtttac tcctgaaaat
    48421 gcggataaag ctgttgaagt gacgggtgtg atacctatat cctatacagg agcttcagga
    48481 atgtttgtag gtatatttgt tggattagcg gctacagaat tatttattaa actttctaat
    48541 aataagaaac tacagattca gctatccgga aatattcctc ccgctgtttt aaagtctttt
    48601 aatgtattaa tcccaatcat tattacgatt acctcgtttg ctttattgtc atttataatc
    48661 attcaattgt ttaatatgga tataaataag ctaattacca cgatagttac agagccatta
    48721 agtaaaatta caactggttt accaggattt ttgttaatta catctattgc taatctattt
    48781 tttggatttg gtatccatca agcagtaata tcaggttcat tgttagatcc gtttttgatt
    48841 cagaatatgc aagaaaatat ggcagcttat gcaaacaaag agcaaattcc acatattatc
    48901 aatatggcct ttaaagatac ctttgcggta atgggtggat cgggaaatac gattgcttta
    48961 ttgattgcaa tatttatctt cagtcgtaga aaagattata aggactttgc taaattatcg
    49021 gtaactcccg ctatattcaa tataagcgaa cctattattt ttggattgcc tattgtattt
    49081 aatatcagtc tgattattcc ctttgtgctt gcaccgattt tttctttgac agtagcatat
    49141 tttgcaactg ctgtgggatt aattaatcat gtagtagtgc aaattccttg gactacgcca
    49201 ccagttatat cgggattttt agcaacagcc ggagactggc gagcagctgt tttacaaata
    49261 ttaattatag ccgcaagtgt gtttatatat ttaccgtttt tacgaattga cgaacacgtg
    49321 acaactaaat taagtgaaaa tgaaacataa tttactatga gataacaaaa attaacacaa
    49381 gaagtcgttg gaatataaat agccagtaat ttcaataagg atcatatttt ttgataagta
    49441 cgaacatgta gaaaaacaaa cattttttgg taaggataat cattattttt cacaagacaa
    49501 acctatctgt gtatatacgt ctatttagta tatgcaaaga taggtttttg gtggtgaaaa
    49561 ataaagactg t
//
LOCUS       DAXLPI010000008        49483 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXLPI010000008 DAXLPI010000000
VERSION     DAXLPI010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.008"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(271..1998)
                     /locus_tag="ACF3T5_000375"
     CDS             complement(271..1998)
                     /locus_tag="ACF3T5_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFD3631014.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1991..3184)
                     /locus_tag="ACF3T5_000376"
     CDS             complement(1991..3184)
                     /locus_tag="ACF3T5_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFD3631015.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3371..4015)
                     /locus_tag="ACF3T5_000377"
     CDS             complement(3371..4015)
                     /locus_tag="ACF3T5_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3631016.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4109..4243)
                     /locus_tag="ACF3T5_000378"
     CDS             complement(4109..4243)
                     /locus_tag="ACF3T5_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFD3631017.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4245..6377)
                     /gene="feoB"
                     /locus_tag="ACF3T5_000379"
     CDS             complement(4245..6377)
                     /gene="feoB"
                     /locus_tag="ACF3T5_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFD3631018.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6374..6847)
                     /locus_tag="ACF3T5_000380"
     CDS             complement(6374..6847)
                     /locus_tag="ACF3T5_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFD3631019.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7248..7472
                     /gene="nrdH"
                     /locus_tag="ACF3T5_000381"
     CDS             7248..7472
                     /gene="nrdH"
                     /locus_tag="ACF3T5_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFD3631020.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7631..9790
                     /gene="nrdE"
                     /locus_tag="ACF3T5_000382"
     CDS             7631..9790
                     /gene="nrdE"
                     /locus_tag="ACF3T5_000382"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFD3631021.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9840..10805
                     /gene="nrdF"
                     /locus_tag="ACF3T5_000383"
     CDS             9840..10805
                     /gene="nrdF"
                     /locus_tag="ACF3T5_000383"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFD3631022.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10894..12033)
                     /locus_tag="ACF3T5_000384"
     CDS             complement(10894..12033)
                     /locus_tag="ACF3T5_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFD3631023.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12342..13055)
                     /locus_tag="ACF3T5_000385"
     CDS             complement(12342..13055)
                     /locus_tag="ACF3T5_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFD3631024.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13309..14010)
                     /gene="nagB"
                     /locus_tag="ACF3T5_000386"
     CDS             complement(13309..14010)
                     /gene="nagB"
                     /locus_tag="ACF3T5_000386"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFD3631025.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14244..15776
                     /locus_tag="ACF3T5_000387"
     CDS             14244..15776
                     /locus_tag="ACF3T5_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFD3631026.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15967..17169)
                     /locus_tag="ACF3T5_000388"
     CDS             complement(15967..17169)
                     /locus_tag="ACF3T5_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3631027.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17251..17940)
                     /locus_tag="ACF3T5_000389"
     CDS             complement(17251..17940)
                     /locus_tag="ACF3T5_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFD3631028.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18046..18414)
                     /gene="rplL"
                     /locus_tag="ACF3T5_000390"
     CDS             complement(18046..18414)
                     /gene="rplL"
                     /locus_tag="ACF3T5_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFD3631029.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(18467..18970)
                     /gene="rplJ"
                     /locus_tag="ACF3T5_000391"
     CDS             complement(18467..18970)
                     /gene="rplJ"
                     /locus_tag="ACF3T5_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFD3631030.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19174..19301)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19380..20069)
                     /gene="rplA"
                     /locus_tag="ACF3T5_000392"
     CDS             complement(19380..20069)
                     /gene="rplA"
                     /locus_tag="ACF3T5_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFD3631031.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20173..20595)
                     /gene="rplK"
                     /locus_tag="ACF3T5_000393"
     CDS             complement(20173..20595)
                     /gene="rplK"
                     /locus_tag="ACF3T5_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFD3631032.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            20938..21606
                     /gene="sdaAB"
                     /locus_tag="ACF3T5_000394"
     CDS             20938..21606
                     /gene="sdaAB"
                     /locus_tag="ACF3T5_000394"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFD3631033.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            21619..22491
                     /gene="sdaAA"
                     /locus_tag="ACF3T5_000395"
     CDS             21619..22491
                     /gene="sdaAA"
                     /locus_tag="ACF3T5_000395"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFD3631034.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(22582..23682)
                     /locus_tag="ACF3T5_000396"
                     /pseudo
     CDS             complement(22582..23682)
                     /locus_tag="ACF3T5_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(23774..24661)
                     /locus_tag="ACF3T5_000397"
     CDS             complement(23774..24661)
                     /locus_tag="ACF3T5_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD3631035.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(24738..25283)
                     /gene="nusG"
                     /locus_tag="ACF3T5_000398"
     CDS             complement(24738..25283)
                     /gene="nusG"
                     /locus_tag="ACF3T5_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFD3631036.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25384..25554)
                     /gene="secE"
                     /locus_tag="ACF3T5_000399"
     CDS             complement(25384..25554)
                     /gene="secE"
                     /locus_tag="ACF3T5_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFD3631037.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(25578..25730)
                     /gene="rpmG"
                     /locus_tag="ACF3T5_000400"
     CDS             complement(25578..25730)
                     /gene="rpmG"
                     /locus_tag="ACF3T5_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFD3631038.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            25892..26536
                     /gene="cbpA"
                     /locus_tag="ACF3T5_000401"
     CDS             25892..26536
                     /gene="cbpA"
                     /locus_tag="ACF3T5_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFD3631039.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(26592..27095)
                     /locus_tag="ACF3T5_000402"
     CDS             complement(26592..27095)
                     /locus_tag="ACF3T5_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFD3631040.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27108..27154)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            27692..28657
                     /locus_tag="ACF3T5_000403"
     CDS             27692..28657
                     /locus_tag="ACF3T5_000403"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFD3631041.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(28756..29928)
                     /locus_tag="ACF3T5_000404"
     CDS             complement(28756..29928)
                     /locus_tag="ACF3T5_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631042.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(29915..31060)
                     /locus_tag="ACF3T5_000405"
     CDS             complement(29915..31060)
                     /locus_tag="ACF3T5_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631043.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31074..31998)
                     /locus_tag="ACF3T5_000406"
                     /pseudo
     CDS             complement(31074..31998)
                     /locus_tag="ACF3T5_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32115..33194
                     /locus_tag="ACF3T5_000407"
     CDS             32115..33194
                     /locus_tag="ACF3T5_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFD3631044.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33191..33819
                     /locus_tag="ACF3T5_000408"
                     /pseudo
     CDS             33191..33819
                     /locus_tag="ACF3T5_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(33929..34156)
                     /locus_tag="ACF3T5_000409"
     CDS             complement(33929..34156)
                     /locus_tag="ACF3T5_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD3631045.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34259..36847)
                     /locus_tag="ACF3T5_000410"
     CDS             complement(34259..36847)
                     /locus_tag="ACF3T5_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFD3631046.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(36867..37526)
                     /locus_tag="ACF3T5_000411"
     CDS             complement(36867..37526)
                     /locus_tag="ACF3T5_000411"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFD3631047.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(37575..38078)
                     /gene="trmL"
                     /locus_tag="ACF3T5_000412"
     CDS             complement(37575..38078)
                     /gene="trmL"
                     /locus_tag="ACF3T5_000412"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFD3631048.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38299..39165)
                     /locus_tag="ACF3T5_000413"
     CDS             complement(38299..39165)
                     /locus_tag="ACF3T5_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFD3631049.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39274..39915
                     /locus_tag="ACF3T5_000414"
     CDS             39274..39915
                     /locus_tag="ACF3T5_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFD3631050.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(39977..41338)
                     /gene="mgtE"
                     /locus_tag="ACF3T5_000415"
     CDS             complement(39977..41338)
                     /gene="mgtE"
                     /locus_tag="ACF3T5_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFD3631051.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41356..42255)
                     /locus_tag="ACF3T5_000416"
     CDS             complement(41356..42255)
                     /locus_tag="ACF3T5_000416"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFD3631052.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42257..43054)
                     /locus_tag="ACF3T5_000417"
     CDS             complement(42257..43054)
                     /locus_tag="ACF3T5_000417"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFD3631053.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43032..43736)
                     /locus_tag="ACF3T5_000418"
     CDS             complement(43032..43736)
                     /locus_tag="ACF3T5_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFD3631054.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            43867..44442
                     /locus_tag="ACF3T5_000419"
     CDS             43867..44442
                     /locus_tag="ACF3T5_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFD3631055.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            44560..45237
                     /locus_tag="ACF3T5_000420"
     CDS             44560..45237
                     /locus_tag="ACF3T5_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFD3631056.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45316..47124)
                     /gene="pepF"
                     /locus_tag="ACF3T5_000421"
     CDS             complement(45316..47124)
                     /gene="pepF"
                     /locus_tag="ACF3T5_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFD3631057.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47300..48298)
                     /locus_tag="ACF3T5_000422"
     CDS             complement(47300..48298)
                     /locus_tag="ACF3T5_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFD3631058.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(48524..49177)
                     /locus_tag="ACF3T5_000423"
     CDS             complement(48524..49177)
                     /locus_tag="ACF3T5_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFD3631059.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        14794 a        10394 c         8723 g        15572 t
ORIGIN      
        1 gtatataaac tatgtatatt ctttaatatt ttttgagaat gcacttcgta cataataaac
       61 tttaatcaca ttagtctttc atacctacga tcgatctctt tgactcgcac ccataaattc
      121 ataatagttt gtcggcgtct ttggacgttt cgaagatgag ctacgcattc tcttttttta
      181 tagacaaaaa agatagagac gaacatggag cttccaaatt tggatatttg atccaatttt
      241 ggaagctcca tttatttcgt gtctgtcttt ttattttttc cagctttcga ctaattcttg
      301 gttctctttg atccagtctt ttgctgcttg ctgtggatct tttccattgt tgatttccaa
      361 cataactttt tccatatctt tttctgacca atggaaattg tctaatactt tgtaagcttc
      421 tggctggtct tctttcaatc cttttcgaac catcgtatgg atctgctctt cgcttcccat
      481 tccgttttca ggatcttcta agtatttcag atcatatttg gcaaacatcc aatgaggtgt
      541 ccatcctgta actacaattg gttcgtgttt tttgatcgct tgacctagtg caacggtcat
      601 tgctcctgaa gaagatgtct ctactttcca gtctttcaag ttgtcatatt tttggatcgt
      661 gttttcagca gccgtaacta caccagctcc tggctcgatt cctatgattt tcttacctgc
      721 ctgatcggtt aactcatcga tcgaatttac atccatgtag gctggcactg ctaaaccaac
      781 ttttgctcct gtcaaattgg cacctaggtc ttcgacttga tctttatatt gtgcatattg
      841 tgaaccatga gttttaggta gccaagctga aaccatggca tctgactcgc catttgaaac
      901 agatttccac atgattgaat tatctaatgg cgtcatcgtt acatcatagc ccatttgttt
      961 caatacttca ccgactacat ttgttgaagc aacttctgta tcccattcaa cataagataa
     1021 gttgatacgt tttgtttctt tggcagatga aaaactccct ataccaatcg cacctaagat
     1081 cactactact acagcaccaa tgatcagtcc ttgacgtttt ttacttttct gtgttgttgc
     1141 tcctgcagct tttttattgt ttggctggtt caaatgctgt gtaaaacggt caatgatgat
     1201 tgctaagata actaatgcaa ccccgttgac aaatccattc ccgacttgtg ctctttgcaa
     1261 tgctgataat acgccgcgtc ctaagccggg tgcaccgatc atagaggcaa taacgaccat
     1321 tgaaagtgac agcattgttg tttgattgat ccctgccatg atcgtacttt tagctaaagg
     1381 caattctagt ttgaatagtt tttgacgact tgtgctcccg aatgaatcag aagcttctac
     1441 taattcttta ggaacttgtc gaatccctaa attcgtaaaa cgaacggttg gtggcagagc
     1501 aaagatgact gaggcaaata cccctggaac catcccgata ccgaaaaatg caactgctgg
     1561 aatcaaataa acgaatcctg gcattgtctg cataaagtca aggatcggtt tgataatact
     1621 ttgggcttta ctgctttttg ccatcaagat ccctaatggt accccaatga tgatcgagat
     1681 cacactagat aaaagaacaa gtgtgacagt attcatcaaa tcattccata gaccttggtt
     1741 ataaataaac catagaccga ttaatgtgaa caacgtaagt cctggacgtt tcttagaaat
     1801 aaagaaagct gctaccgtca ataacaagat aaacaaagga gctggaatca ctaacagtaa
     1861 atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt aaaaatgaaa ataggccaga
     1921 aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag tttgccactg gtaattgata
     1981 gttcaataga ttattcatgt tcactcacct cgcttgtttc tgctaatgct tcaatcacac
     2041 tacctcggat gacgacacct tttaattttc catcctctac aactgcaagt ggagtcggtg
     2101 agtcataaat aatattgaaa atatcactta ccaccatgtc tttcccaatc accgtcacat
     2161 tttcatctag gaagtcgatc aatggacgtt tttgcttcct agcttctaac gcttgatctg
     2221 cagtgatgat ccctttcagg tgtctctttc tgtcaacagc catcagcatg ctgacttctt
     2281 cattacgcat ccttgtcaat gcaacgtttg gtccatcaga ttcgatattc gtcgtcaatg
     2341 caggtaccat gatgttttga gccgtaagca ctttggaacg atcgacttct tccacaaatt
     2401 cacgtacata atcatttgct ggattggtca atatttcttc acctgttccg atctgcatga
     2461 tttcgccgtc tttcatcaag gcgattctgt ccccaatccg taaagcttca ttcaaatcat
     2521 gggtaatgaa aataatcgtt ttttgaacat tggcttgcag gtccagtaat tcgtcttgca
     2581 tttctcgccg gatcagcgga tcaagagccg agaacgcttc gtccatcagc aagatctcag
     2641 gatcatttgc taaagctctg gctaggccaa cccgttgttg catcccacca gataactgac
     2701 taggatattg gtctttaaat gaaagcaaac tagaattttc taatgctttt tctgcttttt
     2761 cttgtcgttc ttctttaggt acaccacgga cttctaaccc gtattctgta ttttccaaga
     2821 tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat attgatttta tgacgacgga
     2881 cttctcgcaa ttcttctttg cttaacttcg atacatcttc tccatcaata tagatactgc
     2941 ccgaagttgg ctcaatcaga cgattcaata gacggatcaa tgtggatttc ccactcccgg
     3001 aaagccccat gatcacgaag atttctcctt cttttacgtc aaaattcacg tcatacacac
     3061 caaccgttgc accggttttc tttaaaatct ccgttttcgg ctggtgatca ttcatcatat
     3121 ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa atgttttact tctactttta
     3181 ccaaacaaat tctccttttt tatgtaaatt catttttctt tctgaaaaaa gtgaccacac
     3241 tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga aatttgcttg tccaaactga
     3301 aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc aagttaatat ttttttaata
     3361 tttcacacaa tcattctgca agctgatgct ccgggtaaaa aaagtttgtc atacgctgta
     3421 gtgtatattc atttccaata aaatgaacga tgtcccctgc cgtaaattta gcataggggc
     3481 caggtgaaac aagcagttct tcattatgag aaattgcgat aactgtggcg ccagtcgcct
     3541 gccagatatt caactcgcta attgaacggt ccagatgcat tgctttttct gataaagata
     3601 attcaaacgg aagtaaagga ttcatcttat gtattcgctt cgtttgattg accagctgat
     3661 ccaataattg agaaaaatga ttcagctcat cttgttgctt ctccacactt gccatgattt
     3721 ctgcttttgt atcttggatg gattgtacat cttgatattg caataggaaa tcttttgctt
     3781 tttccttcga agcaacaaat gccccacttc catgccgtac ttccatgatt ccaagatcta
     3841 ccagcacatt gatagccttg cgggtagtct caggagaagc attgtacgta ctggcaagtg
     3901 ttgagcgggc atggatcttc tcacccactt tatatcggtt ttcaacgata cgttcggcta
     3961 aatcaacagc aatctgctgg tagcgcgggc ggacgatttt tttctttcca gacattttct
     4021 ttcctctttc tttttttact ttccctcatc ttactatgct ctagcaaaat aagcaaaaaa
     4081 ggggagaata gcctcttgca acgactgatc atcgcttgat ctgttccttt tttactgggc
     4141 aagctgtttg acagtcatca caattcttac cggattttaa acgagtatag acaattgcgc
     4201 ctgcacttcc aaagattaac acacttaata agaatgtcgc catcctattc ctctccttct
     4261 aatgacaata tagtataaaa ctggttcttg gattttggct gacggaacaa aacataaacg
     4321 agtcctatca tcagtaaaat agcaagatac gtccctaacg ctacctgtcc accctccaat
     4381 aagtgtccca actgataaat cacaaaacta actaaataag ctaaaccgca ttgatagcct
     4441 atggcgatcc aagtccattt cttggcattc atttcccgat ggatagcccc aattgccgca
     4501 aaacaaggcg cacaaagtaa attgaaggct aacaatgaat aagctgctgc gggagtatat
     4561 gtgtgttgta gcacttgcca gatttctctg ccattttcgg atacttccgc atgaccaaac
     4621 aaaataccaa atgtgccaat aatattttct ttagcgacca atcctgtaat cgctgcgact
     4681 gttccttgcc agtttcccca accaagcggc tgaaaaacaa ctgccaggat tcgcccgaaa
     4741 aaggctaaga tactttggtc ttcagaaaca ggttgagcat aaaaattata agaagagctg
     4801 aaccagatca cgatactaga aacaaagata atcgttcctg cttttttaac aaaagacttg
     4861 ctgcggtcat aggtttgtct taggaccgct gaccattgag gaaaatgata tgcgggcaat
     4921 tccatgataa aaggagctgt atctccagca aacaaacgtg tttttttcaa gctgattcca
     4981 gagaaaataa ttgcagtcat accaagaaaa taggcagaag gagctaccca agattgatga
     5041 ggaaaaaagg cccctgctac taaggcaata atcggcaact ttgcagaaca aggcatgaag
     5101 gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt caatcgtgcg actggccatg
     5161 acgcctggca ctccacatcc tgtagcaatc agcataggaa tgaaggattt tcctgaaaga
     5221 ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc tcgccatata tccgcagtct
     5281 tccagaaagc tcaagcataa aaacaatata atcaattgcg gtagaaatcc taacactgct
     5341 cccacgcctg cgatgatgcc gttcaagatc agctcctgca tccatcccgc tacttgccat
     5401 tcagctaaca atctgccaac atgttctggt aaccatgtac caaacaatgt gtcattgatc
     5461 caatctgtcc ccattgttcc tactgtttga atagccagat aatagatcag ccacatcaca
     5521 aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa tctgatcgat tttgtcactc
     5581 atcgataatt ggaaagaatc atttgacaca acacagagtg tacatagttg ggtaataaat
     5641 tcataacgtt cattgaccag aatcgtctca ctatcgtcgt ccaatagttt ctctgttaac
     5701 tggatcagtt gctcgatctc tttttgctga gaaactgcta tctcgtaatc ctctcttacc
     5761 ttctgatctc gttcaaaata tttcattgca taccagcgtt tcttgccgct ttgttctcct
     5821 agtacatctt caatttcatg taaagctgct tctaaacgtg gatcatatgt acagggaaca
     5881 ggaatagaag gccgcttttt tgcagactcc tgaatctttt cgattagttt ttcgattcct
     5941 tgctttttca atacgctgat tggtaaaact ggtacaccaa gaccatagga aagcttttct
     6001 agattgattt tttttccttc ttttttgatg acatccatca tattcagacc aatgatcata
     6061 ggaatgtcta aatccaataa ctgcgtagtt aaataaagat ttcgttctaa attggtagca
     6121 tcaataatgt tgatgatcag ttcaggctgt tcctgaacca aataatctct tgcaatcact
     6181 tcttctggtg tataaggaga caaagaatag atgccgggta ggtcctgaat atgaatgttc
     6241 ggattttttt tcaacgatcc ttcttttttt tctactgtta cgcccggcca attccctaca
     6301 tattgattcg aaccagtcaa gagattgaac aagctagttt tcccgctgtt tggatttccg
     6361 actaacgcga tattcattct ttttcctccc tttgtatcaa gattagttcg gcctctgact
     6421 ttcgtaacgt cagttcatac cctcgcactg tcagttcgat tggatctccc agaggggcta
     6481 gttttcggat aactattttt acatttcgcg tcaatcccat atccattaac cgtcgcttca
     6541 ctgcgccttg tccgtggatt gctacgacga tcccagtctc tcctgctttt aattgatcaa
     6601 gagaagtcca gctttcagag gaagcagtct ttttattgac aaagatttga cctagtattt
     6661 cccgacttac tgccactcga tgacttttta ccagtaaaat ggcattgttg cctgataaat
     6721 tgacgacgac gattttactt ccggcaacga tcccgatatt ttgcaaatgg cgttttgttt
     6781 tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt ctgctttgcc tgcgctaagg
     6841 tctgcatatt gattcctctc ttgtgaaata aatcatgtat taaaatcatt cattatatgt
     6901 tctaattttc ttacgtgtta cgcaaataaa tgagaatcat tctcacatag aatactctcg
     6961 gtaattgaga atgtcaatta tacttgtgat attatgagcg aatctttagt attgatagat
     7021 aaaaatgaga aaaaaatatt tcttcatagt tttccacaat tttttctcta ttaaatttgc
     7081 tattttccca cacgatactt gttttcagct cttttttcaa aaacacaaaa cttagtattc
     7141 cacagtttcc acaacacaat atgtagtgtt tgttgtttga cttttttact aagagtttta
     7201 tactatctat tatagataat aagaatgtgt tagaggaggc actcaccatg aacgtaaaaa
     7261 tcttttctaa aaataattgt atgcaatgta agatggtcaa acggttttta acagaaaatc
     7321 aaattgcttt tgaagaagtc aatatcgatg aacagcctga agcagtagac tggttgaaag
     7381 cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc agaaacagtt atcggattcc
     7441 gtcctgatca gttgcgtaaa cttgccagct aacatgacta aaagaatcaa aacgtgatac
     7501 atggatttga agggcttgag acagcaccag tttttgtcgc aaacttccaa atcctttttt
     7561 aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca tcaaatacga aatgaaggtt
     7621 gggcgtacgt atgagtctta aaaatttaca agatgtcagt tatttcaagc tgaataatga
     7681 aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag gataaagaag ctttagttgc
     7741 cttttttgag gaaaatgtca aaccaaatac gatgactttt ctatctatta tggaaaaaat
     7801 caattacttg atcgatcaag aatatataga agcagaattc attcaattgt attcaccatc
     7861 atttattgaa aagctatatc aatttttgga cgagcaacat ttttctttca aatcttttat
     7921 ggctgcttac aaattttatt ctcaatatgc gttgaaaacc aacgacggtt ccgcttatct
     7981 ggaaacttat gaagatcgag tagccttcaa tgcgctttac tttgcgaacg gggatgaaga
     8041 attagcactt gctcttgcag acgaaatgat ccatcaacga tatcagccag ctacaccttc
     8101 tttcttgaat gctggacgta aacgtcgggg cgaattggtg tcttgtttct tgactcaagt
     8161 cactgacgat atgaactcta tcgggcgctc aatcaactct gctttgcaat tgtcccgtat
     8221 tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa gccggcgctc ctatcaaagg
     8281 atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa ttattcgaag atagtttcag
     8341 ctattctaat caattaggtc agcgtcaagg agctggcgtg gtttatttga acgttttcca
     8401 tccagacatt attgctttcc tttcagcaaa aaaagaaaat gcagatgaaa agattcgcgt
     8461 aaaaactctt tctcttggcg tgatcgtacc tgataaattt tatgaattga cacgtaaaaa
     8521 cgaagatatg tatttattta gtccatatag tgtcgaaaaa gaatatggtg tcccattctc
     8581 ttatgtaaat attacagctg agtatgatca tctagttgca aatccaaaca ttcgcaaaca
     8641 aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa ttacaacaag aatctggcta
     8701 tccttacgta atcaatattg atacagcgaa ccgcgagaat ccaatcgatg gcaagatcat
     8761 catgagtaat ttatgttcag agattttaca agttcaaacg ccttctgtga ttaacggcaa
     8821 acaggaatat gaagtattag gaacggatat ctcatgtaac ttaggctcga caaatatcgt
     8881 gaatttgatg caaagcccta actttggtca atctgtgcgt acgatgactc gtgcattgac
     8941 atttattaca gatgcatctg atatcgatgt cgtaccaccg atccaaaacg gaaataagct
     9001 gaatcatacg attggattag gtgccatggg cttacacact tatcttgcaa aagaacaaat
     9061 ggaatatggc tcagaggatt cattagactt tacaaatatt tacttcatgc tgttgaatta
     9121 ttggacactt gttgaaagca acaatatcgc tcgtgaaaga aaacaagttt tccataattt
     9181 tgaaaaatca gcttatgctg acggcactta tttcgaaaaa tacgtaactg gagaattcca
     9241 gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt tttatcccaa ctgctgaaga
     9301 ttggaatgct ttgaaacaag cagtaatgaa agatggactg tatcatcaaa atcgcttggc
     9361 agtagcacca aatggctcaa tttcatatat caacgatacg agtgcaagta ttcatcctat
     9421 cactcgcatg atcgaagaac gccaagagaa gaaaatcggt aaaatttatt acccagcgcc
     9481 ttatctatcc aatgagacga ttccgtatta cacttctgct tatgatatgg acatgcgtaa
     9541 agtaatcgat gtttatgcaa tggcacaaca acacgtcgat caaggcatga gtttgacttt
     9601 attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg aagacaacaa cgaagcaaac
     9661 gacacgtgat ttgaatattt taagacatta cgctttccac aaaggaatca aatccattta
     9721 ttacgtacgt acctttactg atgatgcaga agaaatcgga agcaatcaat gtgaaagctg
     9781 cgtgatctaa tatcatagat agattcgctt attcatattt taattaaagg agcattctca
     9841 tgtcagaaac atattatgaa gcaataaact ggaatgaaat tgaagatatc attgataaat
     9901 ccacttggga gaagctgaca gagcaatttt ggctagatac acgtatccca ctttcaaatg
     9961 acctagatga ttggcgtaca ttgtctcaat tggaaaaaga tacagtagga catgtatttg
    10021 gcggtttgac actgctagat actgtccaat ctgaaagtgg catggatcag ttgcgtaaag
    10081 atgtccgcac tccccatgaa gaagccgttt tgaacaatat tcaattcatg gaatccgttc
    10141 atgccaaaag ttattcttct atttttagta cactgaatac gaaaaaagaa atcgaagaaa
    10201 tttttgactg gacaaatacg aatccttatc tgcaaaaaaa agctgagcgt atcaatgaaa
    10261 tctacaaaca cggtacaccg ctagaaaaga aaattgccag cgtcttttta gaaacattct
    10321 tgttctattc aggtttttat actcctcttt attatttagg aaacaacaaa ttggctaacg
    10381 tagcagagat catcaaacta atcatccgag atgaatctgt acatggaacc tacatcggtt
    10441 acaaattcca attaggattc aacgagttac ctgaagcaga acaagaaacg ttaaaagact
    10501 ggatgtacaa tcttctttat gagctttacg aaaacgaaga acgttataca gaagaattat
    10561 acgatccaat cggctggaca gaagaagtca agacattcct acgctataat gccaacaaag
    10621 cattgatgaa cctagggatg gatccactct ttccagatac tgccaatgat gtcaacccaa
    10681 tcgtaatgaa cgggatttca acaggcacaa gcaaccatga cttcttctca caagtcggaa
    10741 acggctattt gttaggtcat gtagaagcga tgaaagacga cgattatttg attggattgg
    10801 aataaggatt tattttaatt caaaaatttt tcgaaaaata aaaagagttt agcgtacaga
    10861 tattttaaat gtacactgaa ctctttttta tgcttattca cttgtttctt ccagcacttt
    10921 tggactgtct aacttttccg aaacatgttc cattcttctc aataaataga ttttccggat
    10981 atttaatatt atcgtcttaa tcactgcata gacaggtatg caaatcagca tgcctaatat
    11041 tcctgcgaca cttcctgctc caatcaatac caagatgatc gtcaaaggat ggatattcat
    11101 tgatttccca aataacaacg gcttgatcaa atttccatct aattgttgga tcactaaaat
    11161 cgacaacgcc atatacaaag cttgaaatgg agagataaac aaaccgacaa tgattgctgg
    11221 taccgctcca ataaaagggc cgacatacgg gataatattc gtgattccgc aaatgaccgc
    11281 aagcaacaag ctataaggct gatcgaatat tctcattgcc aaaaagctca ttacaccaat
    11341 aatcaaagca tctaagatcg tactgctgat ataggcagat aatgtttcgt ttaattcttt
    11401 taccgtttgg cgtaactctt tacgaatcgc tttcggaaag aagtgagaca acgcatcaat
    11461 aaatttgtgc ccatccttga acataaatag caaaataaat ggaacggtaa ataacaaaat
    11521 aaaaaattgc atcataaatg aaatgatttt tgagacactt gcagataaac tgactaatat
    11581 gatattgaac aaattactaa gagataaatt tgctgatgct aattgttctt ccaaattgaa
    11641 atttttaaac tcttcccgct gcgccaattc atttaaccat ttgcttgttt catctgcata
    11701 cattggcaag ctctgggtca attgaattgt ctgttccatt aactgaggca ctacattcat
    11761 gacagttaaa acaactaaaa tgattacgac caaaaacgtc aataaaaaac cgaacactct
    11821 cgggatccga tgcttttcga gaaaaacaac gattggatcg aacatataat agataaaacc
    11881 agcaacaagc aatggtaaaa aaactgccga aagcattttc aatattggac taaaaatatt
    11941 cggcatttgt aaaataaagt acacgccgat gatcgtgagt actagctcca ctgtccagaa
    12001 aaataatttt gattgtttaa aacgtgattg catacatctc ctccaaggtc ctttgatact
    12061 tctattctat catttaacaa agagttacgc gctgagattt tcatttgctt aaaaaaaaca
    12121 aaaaaaataa acatatttta atgagtagac gagtacttaa acaaaaacac ggtttggagt
    12181 agtgacaaaa attatactgc agtccctttt tccaaataaa cggtgttcaa aagcttgcat
    12241 cttcgtaata agcccagcca aacgaaaaat ataaggaact atttttattt ggctgggctt
    12301 attacttgat gtaggacatc tattttacaa cctcagatca tttattgcgc aatactgact
    12361 aaatgcaact ttgtgatatt tttgtgtacg gacaactcct cgatcacatc ggcataagtg
    12421 agcccttttg gaagatcaat cgtatagata tttttgtaca ctcggtagtc atcaacgaat
    12481 tttacgtcaa aattcacatc ttcgattacg atatttttct cttcaaaata atcggtaaga
    12541 aaatctttgg tttcttttct atggataaat tggatttcta actttttagt cgtaggtaca
    12601 tggatcacgc gtttcactac tgtgagtgca atagcaatcc cgataaaact gatgatcgca
    12661 atggaatagt aacccatgcc gatggcgatc cctaatccag caattgccca taaagaagcg
    12721 gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga tcgttcccgc gcctaagaaa
    12781 ccgacaccac tcaccacttg agcaatcagc cttgcttcat ctgaacgaat cgttccagca
    12841 agctgtggat actctaaagc atctcttaat gcaccactag cgatctcaac ttggatcaat
    12901 gcaatgatac atgctcccat acaaactaaa atatgcgtac gcatacctgc tggacgattt
    12961 ttatattgtc gttcaaaacc gattgctccg ccaaatagca tagcgatccc taaacgtaaa
    13021 ataatctctg gaattgtcaa taccatatca ttcatctaaa caccctccag tccaatatat
    13081 catgtctgaa aggagcagac aaaaaaagtg caatttttac ctcttacgaa aaaaggactg
    13141 tgacaaaatt tttgtcatag tcctttttat gaataaacgg tgttcaatca gctgaccctc
    13201 ggtattactc aacaaacggc aaaatatggg gagctatttt tcctttgtct tctctaatac
    13261 tcaggtcaaa acgctgattt cacaacctct ttagcctact attgaaaatt acaacttact
    13321 tgctgcttct tcatcgatga tcacaacaac attgtcgtga ttttgcaata cacttgctgg
    13381 caaatgattc gttacaggtc cttcaatcat ccctttgatt gcatcagctt tgctttcgcc
    13441 aaatgccatc aagacgattt ctttactttt cataattgaa ccgattccca ttgaaacagc
    13501 ttttgttggt acatcttctg ctttatcgaa gaatcgtttg tttgcattga ttgttgattc
    13561 tgttaagtca actactgaag tcgttccgtc aaatgaagca ccaggttcat tgaaaccgat
    13621 atgtccattt tgcccgattc ctaagatttg gatatcgatt ggatgtgcat cgatgatttg
    13681 atcatacttt ttgcatgctg cttccagatc ttctgctttt ccatctggta caaacgtttc
    13741 tttgaatggt tttttatcaa ataattggac atccatgaaa tgacggtagc tttgttcatc
    13801 tgaaccgcct agtccaacgt actcatctaa gtttacagaa gtcatttcac tgaaatccaa
    13861 atcgcttgaa gtcatttctt tatataaagt aactggtgtg cttcctgttg ccaatcctaa
    13921 tacttttgca cctttgttca taccttcttt gatgatttca aatgcttttt ttccgccttc
    13981 ttcagcgttt tttacacgaa tgatttccat agtgattgct cctttatatt ttggtatagc
    14041 ccaattatag cattcttttt ttctttttac aataattagt atagaccaaa ttgcattttt
    14101 ttcattttct tgacaataaa taggaatccg tgttaggttt cataagatgg ttcaatcaaa
    14161 caatccatgt tgcatgaaaa ccaagcaagt gattcactgt ggacgcttta tgtctgcagt
    14221 ttttttatga aaaaggagtt attatgagta tactgacatt aaacaacatt acccagcaat
    14281 ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt aaatgctggc gaacatcttg
    14341 ggctgattgg acaaaacgga gccggtaaaa gtacattgat taaaatcatc actggtgaga
    14401 tcttaccaga tgacggacag atccattggc aaaaaaatat tcacaaaggt tacctcgatc
    14461 aatatgtgga agtcgatgaa acattaacaa tcgaagaatt tctaaaaact gcgtacgcaa
    14521 aagaatacga aaaggaagaa aaaattgctt ttctttacca aacatatagc gagaccttcg
    14581 atgattcctt gctggaaaaa gctgggaaac tccaaacgga attagaccaa ggagtgtttt
    14641 atcaaatcga caccttagtt gctgagatga gcagcgggtt ggggatcgat gtcttaggtt
    14701 tagattcact gttgaaaaat ctaagtggtg gacaacgttc aaaggtgatt ttagcaaaac
    14761 tacttttaga aaacccagat gttctgatct tagatgaacc aaccaatcac ttagatgatc
    14821 agcatatcca atggctgacg cagtttcttc aggattttga cggtgcgtat atcgtgattt
    14881 cccatgacca agaatttctt gatcagatca ccacccatat cgcagacatc gagtttggaa
    14941 agattactaa gtatactggc catctaaaac aagcattgaa acaaaaggaa caaaatcgcg
    15001 aaagctattt gcgccaatat catgctcaac aaaaacaaat cgaaaagacc gaagcttata
    15061 ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa aagccgacaa aaacaattag
    15121 ataaaatcga acggctgacg cctccagctt cagcaagtaa agctgtcttt gattttccat
    15181 tctcgcctat tgtcacgaca ttagcagtgg aaacaactga tttagtcatc ggatatgatc
    15241 gaccgctact tgcacccatc aatttgacca tccgctttgg tgaaaatatt gccattcgag
    15301 gcttcaacgg gattggaaaa tctactttat taaaaacact gatcggtgag attgaaaaaa
    15361 taagtggcga tttccacttc ccagataaca caaaaatcaa ttacttttca caagatctgt
    15421 actgggaaaa tccattagaa acaccactac aatatttaag cagtcaattt ccaaaagcga
    15481 ctatcaaaga attacgtcgc caactagcta aagcagggtt agttaatcaa ttagcaagtg
    15541 aacctctcac tacattaagt ggtggggaac aaactaaggt gaaacttgca cagttgacaa
    15601 tgaaccaagg taatttactg attctcgatg aaccgacgaa ccatatcgat caagaaacga
    15661 aagaaagctt acaagacggg atacagaaat ttccaggaac agtaatcatc gtctctcatg
    15721 aacaagaatt ttatcaagat cttgtgcaac gagtcattga gattgaacca aaataaacgt
    15781 ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt ttgtcacaac tccttcttct
    15841 gaataaatgg tggccaatta gctgaccttc ggtattagct caaaaaacgg caaaatatga
    15901 ggagctattt ttatttggct attcttatta cttgatgcag aacagctttt cacaacctct
    15961 tttcgtctat tttttatttt cgagatttct ctttccttta aaaacaactg tttgaaagaa
    16021 gaacgcacat ccttgaaaaa caagcccgct aaccgttaca cctaaccagc catactgctg
    16081 ccacattgtc gtccctagca atgaaccagc cgcacctcca ataaagtaca taaacataaa
    16141 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
    16201 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
    16261 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
    16321 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
    16381 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
    16441 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
    16501 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
    16561 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
    16621 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
    16681 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
    16741 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
    16801 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
    16861 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctgc
    16921 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
    16981 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
    17041 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
    17101 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtt ttttttcatt
    17161 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
    17221 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
    17281 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
    17341 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
    17401 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
    17461 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
    17521 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
    17581 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
    17641 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
    17701 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
    17761 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
    17821 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
    17881 taccagtatc agaatgattc ccaacgagat caatatcaat cggattattt tctttttcat
    17941 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
    18001 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
    18061 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
    18121 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
    18181 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
    18241 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
    18301 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
    18361 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
    18421 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
    18481 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
    18541 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
    18601 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
    18661 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg
    18721 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
    18781 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
    18841 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
    18901 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
    18961 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
    19021 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
    19081 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
    19141 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
    19201 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
    19261 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
    19321 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
    19381 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
    19441 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
    19501 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
    19561 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
    19621 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
    19681 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
    19741 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
    19801 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
    19861 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
    19921 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
    19981 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
    20041 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
    20101 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
    20161 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
    20221 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
    20281 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
    20341 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
    20401 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
    20461 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
    20521 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
    20581 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
    20641 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
    20701 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
    20761 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
    20821 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
    20881 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
    20941 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
    21001 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
    21061 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
    21121 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
    21181 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
    21241 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
    21301 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
    21361 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
    21421 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
    21481 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
    21541 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
    21601 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
    21661 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
    21721 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
    21781 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
    21841 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
    21901 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
    21961 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
    22021 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
    22081 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
    22141 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
    22201 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
    22261 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
    22321 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
    22381 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
    22441 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
    22501 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
    22561 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
    22621 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
    22681 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
    22741 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
    22801 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
    22861 cgcaggacct ccatctagtt gacgtccacc atgattggat acccagatac catctgcccc
    22921 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
    22981 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
    23041 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
    23101 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
    23161 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
    23221 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
    23281 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
    23341 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
    23401 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
    23461 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
    23521 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
    23581 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
    23641 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
    23701 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
    23761 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
    23821 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
    23881 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
    23941 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
    24001 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
    24061 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
    24121 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
    24181 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
    24241 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
    24301 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
    24361 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
    24421 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
    24481 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
    24541 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
    24601 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
    24661 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
    24721 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
    24781 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
    24841 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
    24901 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
    24961 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
    25021 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
    25081 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
    25141 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
    25201 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
    25261 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
    25321 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
    25381 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
    25441 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
    25501 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
    25561 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
    25621 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
    25681 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
    25741 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
    25801 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
    25861 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
    25921 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
    25981 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
    26041 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
    26101 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    26161 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    26221 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    26281 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    26341 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    26401 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    26461 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    26521 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    26581 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    26641 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    26701 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    26761 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    26821 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    26881 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    26941 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    27001 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    27061 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    27121 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    27181 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    27241 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    27301 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    27361 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    27421 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    27481 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    27541 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    27601 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat
    27661 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
    27721 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
    27781 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
    27841 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
    27901 cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga
    27961 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaga
    28021 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
    28081 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
    28141 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
    28201 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
    28261 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
    28321 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
    28381 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
    28441 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
    28501 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
    28561 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
    28621 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
    28681 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
    28741 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
    28801 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
    28861 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
    28921 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
    28981 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
    29041 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
    29101 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
    29161 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
    29221 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
    29281 gtttcgttta ctgatctctt tgcgattaaa tgctagattg atgacgctga aaccgctaaa
    29341 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
    29401 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
    29461 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
    29521 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
    29581 tatcttgatc gtcggcatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
    29641 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
    29701 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
    29761 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
    29821 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
    29881 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
    29941 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
    30001 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
    30061 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
    30121 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
    30181 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
    30241 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
    30301 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
    30361 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
    30421 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
    30481 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
    30541 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
    30601 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
    30661 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
    30721 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
    30781 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
    30841 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
    30901 ttccagtatt aatcgtttcg agattgccac cttgatcaat ctcgccaaag gccgccataa
    30961 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
    31021 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
    31081 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
    31141 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
    31201 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
    31261 attgttcgct gacaacacgt tttctgggag taatgggatg tcgatcgtca aacgatccgc
    31321 cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct gtccttgatc
    31381 catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg ttgtatagac
    31441 aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat tccgactttg
    31501 ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt gggcaatccc
    31561 acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac gaaacgaaac
    31621 atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc ttttctcttt
    31681 ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta gttcgtcaaa
    31741 gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat tttcaggagt
    31801 cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag aaagcataca
    31861 gttgatggca gtcgatttcc cactgccgtt tggtcctaac agacctaaga cttctccttc
    31921 ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt tgactaattt
    31981 atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat ctgactataa
    32041 taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca tttgtcacaa
    32101 tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt ctactggtta
    32161 tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc tatttattga
    32221 tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat tggattttcc
    32281 aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt ctgccagcga
    32341 cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg ttaggttttt
    32401 tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc gtaattttgc
    32461 tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt gagaaaaagt
    32521 acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa caaaataaag
    32581 cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa cggaaccgaa
    32641 ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt ttacaaattg
    32701 gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa aatttaagtg
    32761 ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc catgagacat
    32821 cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct tctgttacga
    32881 ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc ttaatggtca
    32941 tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt gttttacatt
    33001 ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc agcccacaaa
    33061 aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa gaacaaaagg
    33121 gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt ttaccaaggg
    33181 agggacacca atgataacag tagtcattat tgagatgatc catttgtgac gaaatcgtta
    33241 caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca ttgtgccaag
    33301 gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    33361 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    33421 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    33481 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    33541 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    33601 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    33661 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    33721 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    33781 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    33841 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    33901 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    33961 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    34021 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    34081 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    34141 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    34201 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    34261 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    34321 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    34381 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    34441 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    34501 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    34561 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    34621 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    34681 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    34741 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    34801 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    34861 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    34921 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    34981 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    35041 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    35101 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    35161 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    35221 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    35281 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    35341 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    35401 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    35461 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    35521 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    35581 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    35641 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    35701 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    35761 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    35821 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    35881 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    35941 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    36001 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    36061 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    36121 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    36181 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    36241 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    36301 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    36361 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    36421 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    36481 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    36541 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    36601 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    36661 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    36721 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    36781 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    36841 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    36901 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    36961 gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa ctcacttagc
    37021 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    37081 agtgggccat ctttatgttg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    37141 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    37201 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    37261 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    37321 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    37381 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    37441 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    37501 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    37561 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    37621 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    37681 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    37741 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    37801 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    37861 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    37921 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    37981 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    38041 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    38101 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    38161 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    38221 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    38281 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    38341 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    38401 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    38461 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    38521 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    38581 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    38641 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    38701 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    38761 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    38821 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    38881 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    38941 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    39001 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    39061 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    39121 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    39181 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    39241 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    39301 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    39361 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    39421 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    39481 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    39541 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    39601 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    39661 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    39721 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    39781 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    39841 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    39901 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    39961 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    40021 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    40081 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    40141 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    40201 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    40261 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    40321 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    40381 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    40441 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    40501 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    40561 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    40621 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    40681 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    40741 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    40801 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    40861 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    40921 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    40981 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    41041 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    41101 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    41161 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    41221 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    41281 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    41341 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    41401 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    41461 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    41521 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    41581 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    41641 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    41701 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    41761 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    41821 ctttgcaaaa agcatcaaac cagatgtatc ccgatctagt cgcgtcacca catggattac
    41881 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    41941 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    42001 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    42061 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    42121 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    42181 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    42241 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    42301 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    42361 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    42421 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    42481 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    42541 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    42601 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    42661 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    42721 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    42781 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    42841 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    42901 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    42961 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    43021 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    43081 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    43141 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    43201 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    43261 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    43321 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    43381 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    43441 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    43501 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    43561 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    43621 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    43681 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    43741 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    43801 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    43861 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    43921 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    43981 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    44041 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    44101 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    44161 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    44221 accggaaagc ttacaacaaa acgggctgaa ttccctattg aagaaggact tttagctatt
    44281 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    44341 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    44401 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    44461 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    44521 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    44581 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    44641 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    44701 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    44761 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    44821 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    44881 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    44941 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    45001 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    45061 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    45121 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    45181 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
    45241 aatacgtcga ctcccttaca agagagccga cgtatttttt tcgtttctat tcgagaaaat
    45301 caaaaataga aagccttata aacgatcgat caattcttct aattcattta gacgttgttc
    45361 aaacatcgac attgcatctt caatatatgc agcctgtgtc atatcaactc ctgctttttt
    45421 cattacttcc acaggataat cgctgttacc tgcttttagg taagccaagt agttctctag
    45481 tgcctctggt tcctggttta gtattttctt cgccagtgct gaagcagcag aaaagccagt
    45541 cgagtattgg aaaacatagt aattataata aaaatgcggt atccgtgacc attcgaattt
    45601 aatttctggg tcttcttcga ctgctggacc ataatatttt gcattcaatt taccataact
    45661 atcacttagg tattcactag tcaatggcac acctttttca tcttccgtat gcatgaaatg
    45721 ctcgaattca gcgaattgtg tctggcggaa aactgttcct ttaaacccat ccaagtaatg
    45781 gttgagtaca taagcccgta cacgagggtc tttttctgtt tccaataaat actccgttag
    45841 gatattttca ttcgttgtcg aagcaatttc tgctaaaaag atggagtagt cgccgtacac
    45901 ataaggctgg ttcgaacgag tgaaataact atgaacacta tgtcccattt cgtggacaag
    45961 cgtaaatagc tgatccagcg tatcatgcca attcaataaa atatatggat ttgtgtcata
    46021 gcttcccgaa gaataagcat cgcttcgttt ccctttattt tcgacaacat cgatccaacg
    46081 ttcagagaat gctttttcta cgatggccat gtattcttca cccattggtt tcagtgcttc
    46141 taaagctttt tcttttgctt cttcgtacgt aaaggtaatt ggagcttccc ctaagaccgg
    46201 tgtataaaga tcatacatgt gcagtttttc cacttctaat aaacgtttcc gtaattccat
    46261 gtatcgatgt aacaaaggca aatgcttgtt taccacgtct accaaagtat cgtatacact
    46321 ttcaggaata tgattattgc tcaaagacgc ttctctggca gagctgtaat tacggacttt
    46381 cgctttaaaa ttatgtcctt ttatatgtgt gcctaaagta gaagcaaatg tatttctaaa
    46441 ttgttcgtaa acactgtaca atcccttaaa tgctgcttcg cgcacccttc gatccgtgct
    46501 ttctagcaac tgaccataca cgccatgaga taattggact atttcaccat tttctccttc
    46561 aatcgttgga aaaactagat ctgcattatt caaaacagca aatgtatcac ttgaagcatc
    46621 aaagatttca cctgctccag caagaagaga ttcctgctca gcagataaga catgggctcg
    46681 attatctact atttgctgga tataatggcg atagacttcc aattttggtt cttctttgaa
    46741 atattgccaa atctggtcat ctgacaattg caatatttct ggttcaaacc aggaaacggc
    46801 ttcactaact ttcgaaaaca gactgcttgc tcttgcataa agcgcttggt aatctgtatt
    46861 tccagtgtct tgatcgtttt taaggtgcgc atatacataa atgacttcag tttggcgata
    46921 aaccctcaat acgaattcaa tcgcatttaa aaattgagaa gcgccttgat caagtgttcc
    46981 tttgtgtttt tcagattgtt ttaactcttc tgataattcc aaatatttct catcaaactc
    47041 ttggtcgctt gaaaagatct tggtcaagtc ccaagttaaa ttttcaggca attcttctcg
    47101 ttttggcaac tgctttactt ccatgaaatc cactcctttt cttcccttag ctctatctat
    47161 caaaatagat catagtctaa catccacatg tcacagcatc taacttttct actcattcat
    47221 gtgtctgctt ccatcaattt tagcataact aaatcttgtt ttacatagaa aaccgattcc
    47281 tagcctattt ttatacttac tattttttca aagaagacaa gtgtatgcat tcgttaaaaa
    47341 actgctgata gaccatctct tcatcaatca taggaaaaag ccattccctt ttatgttctt
    47401 ttatttggca aagccatgag ttgaaatcag gaactttctg gttttccaag gcttcttcgt
    47461 acaacattcg ataaagaaaa acctgttctt gtaaataaaa gaaaaaccga cttttttgat
    47521 agatccacga tgatagatgc aaaagatgtt tatgacgcaa gtagcattgt tcttgtatgg
    47581 atactgtatt cttttgcttg tgaataagtt ggcgtgtcag ccatttcttc ccatcttcac
    47641 tcactttttc tatttttttc atttggttca ttttaatttc gttactagtg aatatttctt
    47701 taaggtccct actagaaaaa gtccagcagc tactgcaaaa ggaaatatgc cctgatacag
    47761 tctctgtaat atgatgatac aaataaagct tttgtttctt gagatcagct ttccagcaat
    47821 gcactcctct ttttctatta tagtagcagc aacttttttc tatcaatgag aattgcgatt
    47881 gtccaagaaa agaattacca agtatccacc atacttggta actgttattc aaataatttt
    47941 gggttctttc aataaatctc tggtgagaca gtctagaaca ttggatttct attgctagat
    48001 tcgccaacaa aatatccggc ctttgccgca acctcggcaa ataggcttcc aaaaatactg
    48061 gttctactga ctgtcccagc caatccatga agcattcctt taactgtaag tgctcttttg
    48121 attctctttc actaaagacc gaacagttat ctgatttttg atgggcaaaa tgagcacatt
    48181 ttttctttcc ctttttcaaa atcactcttt ttcgacagcc acgacaataa tatttctttc
    48241 ctttttctgc atgtttagca aaacagcact tattatattc attcattgca agcagcattt
    48301 ttctccctct gaacttattt acgtctaaga atttatttac acgaaaaaag agggcgtgaa
    48361 aaagctattc tgcatcaagt aataagaaca gctaaacaaa aatagttccc aatatttttc
    48421 gtttggttgg gcttgttacc gcagatgcaa gcttttgaac acatttatac gaaaaaaaga
    48481 ctgcgacaca atttttgtca cagtctctta ctataatgct gatttataca aaataaaatt
    48541 tcgttcgctc gatagcatta cgcgccatca gacattgtcc atattcattc aatgtttctg
    48601 gtgtacgatc gctcttttca gcaaactcca aaattcgagc aatctgattc tctacatccg
    48661 cttggttgag attttccatc cagaagtgaa cgactaggta atagcgctct tcgaataaat
    48721 acaaatcagt ccaagcagac tctagttcga catctgttgc aagttggatc acttgttcaa
    48781 aatcttcaaa agcaaaagtt gcttgtcttt cctgttcttc tagggcgctt ccagataatt
    48841 cttcctccgg atcgctgcca ataatctgct gacgaagaaa gtcactcaca tcttctgaat
    48901 caacagtaga atcttctgat tgagaaattt cttcatccgg aagattttta ctgataaaca
    48961 gttctaatcc gtctcctttt ggcaaaactt ggaaagtgac agcttcgctt cctcggaact
    49021 cctcttcgat atcaacttct tccaaaatac tataaaagaa attttcgatt tctttgtgat
    49081 tgccaagtaa atccaaaaat gaaacgcccc gttcctctaa atcttcatga ccaattagta
    49141 cacggatcgt attttcatta atatgttcca tttccatttt gctacacctc actttgccac
    49201 aatgtttaat atagattcat tgtagcgaaa tcagcttaaa tgtaaagaaa aaaaatctcg
    49261 ctgttaagcg aatttaataa aaccagcgga tttttcaacc tttcaagcta tcttatttaa
    49321 ctataacatt tttggcagac gaatactata tagccgactt ttcagctaat tttccctctc
    49381 tatttttcgt gcaatcatca tcttctacta cctttttata catctaatca agaatacttt
    49441 ttgtaaaaac aaaaaaactt ggaaaaacta tctttgtaat agt
//
LOCUS       DAXLPI010000009        46219 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXLPI010000009 DAXLPI010000000
VERSION     DAXLPI010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.009"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            160..840
                     /locus_tag="ACF3T5_000424"
     CDS             160..840
                     /locus_tag="ACF3T5_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFD3631060.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="ACF3T5_000425"
     CDS             930..1964
                     /locus_tag="ACF3T5_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFD3631061.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="ACF3T5_000426"
     CDS             1978..2313
                     /locus_tag="ACF3T5_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631062.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="ACF3T5_000427"
     CDS             2297..4777
                     /locus_tag="ACF3T5_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631063.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="ACF3T5_000428"
     CDS             4833..6392
                     /locus_tag="ACF3T5_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3631064.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="ACF3T5_000429"
     CDS             6389..7753
                     /locus_tag="ACF3T5_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFD3631065.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="ACF3T5_000430"
     CDS             7924..8994
                     /locus_tag="ACF3T5_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631066.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="ACF3T5_000431"
     CDS             9009..9677
                     /locus_tag="ACF3T5_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631067.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="ACF3T5_000432"
     CDS             9697..10275
                     /locus_tag="ACF3T5_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3631068.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="ACF3T5_000433"
     CDS             10676..11587
                     /locus_tag="ACF3T5_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFD3631069.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACF3T5_000434"
     CDS             complement(11965..12882)
                     /gene="rnhC"
                     /locus_tag="ACF3T5_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFD3631070.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="ACF3T5_000435"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="ACF3T5_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFD3631071.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="ACF3T5_000436"
     CDS             13579..14127
                     /locus_tag="ACF3T5_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFD3631072.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="ACF3T5_000437"
     CDS             14242..16602
                     /locus_tag="ACF3T5_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFD3631073.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="ACF3T5_000438"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="ACF3T5_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFD3631074.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="ACF3T5_000439"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="ACF3T5_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFD3631075.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="ACF3T5_000440"
     CDS             19108..20007
                     /locus_tag="ACF3T5_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631076.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="ACF3T5_000441"
     CDS             20000..21262
                     /locus_tag="ACF3T5_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631077.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="ACF3T5_000442"
     CDS             21423..23321
                     /locus_tag="ACF3T5_000442"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFD3631078.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="ACF3T5_000443"
     CDS             complement(23651..24370)
                     /locus_tag="ACF3T5_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFD3631079.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     misc_feature    24560..24607
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            24707..25039
                     /locus_tag="ACF3T5_000444"
     CDS             24707..25039
                     /locus_tag="ACF3T5_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631080.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="ACF3T5_000445"
     CDS             25026..27029
                     /locus_tag="ACF3T5_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFD3631081.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="ACF3T5_000446"
     CDS             27048..27518
                     /locus_tag="ACF3T5_000446"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFD3631082.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="ACF3T5_000447"
     CDS             27539..28123
                     /locus_tag="ACF3T5_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HFD3631083.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="ACF3T5_000448"
     CDS             28138..29124
                     /locus_tag="ACF3T5_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFD3631084.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="ACF3T5_000449"
     CDS             29114..29425
                     /locus_tag="ACF3T5_000449"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFD3631085.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="ACF3T5_000450"
     CDS             29447..31228
                     /locus_tag="ACF3T5_000450"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFD3631086.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="ACF3T5_000451"
     CDS             31221..32597
                     /locus_tag="ACF3T5_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFD3631087.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="ACF3T5_000452"
     CDS             32604..33239
                     /locus_tag="ACF3T5_000452"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFD3631088.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="ACF3T5_000453"
     CDS             33269..34624
                     /locus_tag="ACF3T5_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFD3631089.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="ACF3T5_000454"
     CDS             complement(34816..36210)
                     /locus_tag="ACF3T5_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFD3631090.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="ACF3T5_000455"
     CDS             36302..37273
                     /locus_tag="ACF3T5_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFD3631091.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="ACF3T5_000456"
     CDS             37270..38052
                     /locus_tag="ACF3T5_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFD3631092.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="ACF3T5_000457"
     CDS             complement(38221..38592)
                     /locus_tag="ACF3T5_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD3631093.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="ACF3T5_000458"
     CDS             38726..39169
                     /locus_tag="ACF3T5_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFD3631094.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACF3T5_000459"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="ACF3T5_000459"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFD3631095.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="ACF3T5_000460"
     CDS             complement(40040..41407)
                     /locus_tag="ACF3T5_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFD3631096.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="ACF3T5_000461"
     CDS             complement(41391..42221)
                     /locus_tag="ACF3T5_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFD3631097.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="ACF3T5_000462"
     CDS             42384..42821
                     /locus_tag="ACF3T5_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFD3631098.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /gene="rpoE"
                     /locus_tag="ACF3T5_000463"
     CDS             42897..43517
                     /gene="rpoE"
                     /locus_tag="ACF3T5_000463"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFD3631099.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="ACF3T5_000464"
     CDS             43706..44212
                     /locus_tag="ACF3T5_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFD3631100.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="ACF3T5_000465"
     CDS             44229..45128
                     /locus_tag="ACF3T5_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFD3631101.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="ACF3T5_000466"
     CDS             45215..46099
                     /locus_tag="ACF3T5_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFD3631102.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        15236 a         7977 c         9766 g        13240 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaatttt
//
LOCUS       DAXLPI010000010        44554 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXLPI010000010 DAXLPI010000000
VERSION     DAXLPI010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.010"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            138..584
                     /gene="nrdI"
                     /locus_tag="ACF3T5_000467"
     CDS             138..584
                     /gene="nrdI"
                     /locus_tag="ACF3T5_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFD3631103.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(649..2631)
                     /locus_tag="ACF3T5_000468"
     CDS             complement(649..2631)
                     /locus_tag="ACF3T5_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFD3631104.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(2645..3541)
                     /gene="murQ"
                     /locus_tag="ACF3T5_000469"
     CDS             complement(2645..3541)
                     /gene="murQ"
                     /locus_tag="ACF3T5_000469"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFD3631105.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(3544..4605)
                     /locus_tag="ACF3T5_000470"
     CDS             complement(3544..4605)
                     /locus_tag="ACF3T5_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFD3631106.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(4816..5343)
                     /locus_tag="ACF3T5_000471"
     CDS             complement(4816..5343)
                     /locus_tag="ACF3T5_000471"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFD3631107.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(5410..6912)
                     /gene="eat(A)"
                     /locus_tag="ACF3T5_000472"
     CDS             complement(5410..6912)
                     /gene="eat(A)"
                     /locus_tag="ACF3T5_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFD3631108.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(7170..8291)
                     /locus_tag="ACF3T5_000473"
     CDS             complement(7170..8291)
                     /locus_tag="ACF3T5_000473"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFD3631109.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            8417..9358
                     /locus_tag="ACF3T5_000474"
     CDS             8417..9358
                     /locus_tag="ACF3T5_000474"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFD3631110.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(9508..10404)
                     /locus_tag="ACF3T5_000475"
     CDS             complement(9508..10404)
                     /locus_tag="ACF3T5_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFD3631111.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            10673..12013
                     /locus_tag="ACF3T5_000476"
     CDS             10673..12013
                     /locus_tag="ACF3T5_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD3631112.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            12231..12674
                     /locus_tag="ACF3T5_000477"
     CDS             12231..12674
                     /locus_tag="ACF3T5_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFD3631113.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(13178..14596)
                     /gene="nhaC"
                     /locus_tag="ACF3T5_000478"
     CDS             complement(13178..14596)
                     /gene="nhaC"
                     /locus_tag="ACF3T5_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFD3631114.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(14999..16222)
                     /locus_tag="ACF3T5_000479"
     CDS             complement(14999..16222)
                     /locus_tag="ACF3T5_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFD3631115.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(16253..17092)
                     /locus_tag="ACF3T5_000480"
     CDS             complement(16253..17092)
                     /locus_tag="ACF3T5_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFD3631116.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(17109..17789)
                     /locus_tag="ACF3T5_000481"
     CDS             complement(17109..17789)
                     /locus_tag="ACF3T5_000481"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFD3631117.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(17791..18864)
                     /locus_tag="ACF3T5_000482"
     CDS             complement(17791..18864)
                     /locus_tag="ACF3T5_000482"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFD3631118.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(18920..19158)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(19201..19434)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19511..20182)
                     /locus_tag="ACF3T5_000483"
     CDS             complement(19511..20182)
                     /locus_tag="ACF3T5_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3631119.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(20269..22917)
                     /gene="mgtA"
                     /locus_tag="ACF3T5_000484"
     CDS             complement(20269..22917)
                     /gene="mgtA"
                     /locus_tag="ACF3T5_000484"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFD3631120.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(23259..23433)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            23606..24043
                     /gene="ndk"
                     /locus_tag="ACF3T5_000485"
     CDS             23606..24043
                     /gene="ndk"
                     /locus_tag="ACF3T5_000485"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFD3631121.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(24065..24931)
                     /locus_tag="ACF3T5_000486"
     CDS             complement(24065..24931)
                     /locus_tag="ACF3T5_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFD3631122.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(25091..27184)
                     /locus_tag="ACF3T5_000487"
     CDS             complement(25091..27184)
                     /locus_tag="ACF3T5_000487"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFD3631123.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(27933..28556)
                     /locus_tag="ACF3T5_000488"
     CDS             complement(27933..28556)
                     /locus_tag="ACF3T5_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631124.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHVGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(28715..30112)
                     /locus_tag="ACF3T5_000489"
     CDS             complement(28715..30112)
                     /locus_tag="ACF3T5_000489"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFD3631125.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(30144..31115)
                     /locus_tag="ACF3T5_000490"
     CDS             complement(30144..31115)
                     /locus_tag="ACF3T5_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631126.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            31489..31914
                     /locus_tag="ACF3T5_000491"
     CDS             31489..31914
                     /locus_tag="ACF3T5_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631127.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(32402..33103)
                     /locus_tag="ACF3T5_000492"
     CDS             complement(32402..33103)
                     /locus_tag="ACF3T5_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFD3631128.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(33119..33559)
                     /locus_tag="ACF3T5_000493"
     CDS             complement(33119..33559)
                     /locus_tag="ACF3T5_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFD3631129.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(33559..34134)
                     /locus_tag="ACF3T5_000494"
     CDS             complement(33559..34134)
                     /locus_tag="ACF3T5_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFD3631130.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            34375..34584
                     /locus_tag="ACF3T5_000495"
     CDS             34375..34584
                     /locus_tag="ACF3T5_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFD3631131.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(34982..35494)
                     /locus_tag="ACF3T5_000496"
                     /pseudo
     CDS             complement(34982..35494)
                     /locus_tag="ACF3T5_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(35620..36321)
                     /locus_tag="ACF3T5_000497"
     CDS             complement(35620..36321)
                     /locus_tag="ACF3T5_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3631132.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(36429..37871)
                     /locus_tag="ACF3T5_000498"
     CDS             complement(36429..37871)
                     /locus_tag="ACF3T5_000498"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFD3631133.1"
                     /translation="MQSRFSEKFLWGGATAANQLEGAYDRDGKGLSVADAMPGGKQRF
                     AIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPN
                     GDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYE
                     RFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVA
                     SSKAVKIGHELRSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGE
                     YPAYTARTHKKYGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSE
                     GNLLGGVKNPFLEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDG
                     TIIDDYRIDYLRRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDL
                     DDEGNGTLNRSKKKSFDWYKEVIASNGVNL"
     gene            complement(37885..39750)
                     /locus_tag="ACF3T5_000499"
     CDS             complement(37885..39750)
                     /locus_tag="ACF3T5_000499"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFD3631134.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(39871..40722)
                     /gene="licT"
                     /locus_tag="ACF3T5_000500"
     CDS             complement(39871..40722)
                     /gene="licT"
                     /locus_tag="ACF3T5_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFD3631135.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(41032..41745)
                     /locus_tag="ACF3T5_000501"
     CDS             complement(41032..41745)
                     /locus_tag="ACF3T5_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFD3631136.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(41813..42139)
                     /locus_tag="ACF3T5_000502"
     CDS             complement(41813..42139)
                     /locus_tag="ACF3T5_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFD3631137.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(42262..42489)
                     /locus_tag="ACF3T5_000503"
     CDS             complement(42262..42489)
                     /locus_tag="ACF3T5_000503"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFD3631138.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(42811..44064)
                     /locus_tag="ACF3T5_000504"
     CDS             complement(42811..44064)
                     /locus_tag="ACF3T5_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFD3631139.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLIGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            44125..>44554
                     /locus_tag="ACF3T5_000505"
     CDS             44125..>44554
                     /locus_tag="ACF3T5_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD3631140.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGS"
BASE COUNT        12798 a         9373 c         7623 g        14760 t
ORIGIN      
        1 aaaaacttta atagaaatat aattctaccg gcttttttct cattatacaa gcaaagcgcc
       61 tagtcccttt ccaaattaca ccaccaaaaa acttaaaaaa gatgctatac taaaaggaaa
      121 gaattggagg atgatccatg aacattttat atatatcgat ttcaggaaat acgcgtgcct
      181 ttgccaaaca tctagcagaa tatgctgaaa aaatgcatgc agaagatcct gtcaatcctg
      241 aagttacttt aaaagaaatc catgaaaata gtgattttac gaaagaaaca gaaccatttt
      301 ttactttcgt tcctacttat ttagacggcg gaaatggttt agataacggt gatacagaaa
      361 tcttgaccga aacgatgcgt gaatatctgg aacatgaaga caatcataaa ctttgtcttg
      421 gtgtagtcgg tagcggaaat aaaaacttca ataatcagta ttgtctaaca gccaaacaat
      481 atgcacaaac atttggcttt cctttcttgg cagattatga attacgggga acaccatctg
      541 atgtgacacg aatttatcag attttagcag aaaacaataa ataataaaaa agaagaagct
      601 gaaaaataga tttcagtttc ttctttcgtt tctattgttt ctatcatatt aagctttgac
      661 atccatcaca ggtgcttttg cttttgcttt acctgttttt tccaagacaa agttttttac
      721 acgatccata ttggttacca agacgatcat ggatgtttct ttgcctgctg ctttgatcgc
      781 ttctaagtca gcttctgcta caagcgtatc aggtgtcact tgctgccctt ctgttacttt
      841 cacatcaaat ggcgtaccgc ctaagtcaac tgtgttgatt cccatatgaa gcaagatttc
      901 taaaccattt ggcgttgtga tcccaatagc gtgtttcgtt gggaagacag agctgactac
      961 cccagtaact ggagaataga tatgtccgtt ttctggttct acaaagtagc cttcacccat
     1021 catcttttgt gcaaaaactg gatcacttgc tttatctaaa gaaacaactg ttccgtcagc
     1081 aactgatgag aggatagtca cactttcagc tgaagtttga ggtactactt tttcaggcac
     1141 ttcatttact gtggctgttt cagtcgctaa ttcttctttt tctaaacggt ctttaggaat
     1201 accgaagaag aatgtagcaa cgaagccacc agcataagct gccaataatc caaggatgta
     1261 gcctaaccat ttaccgtcag agatcaatgg gatcaaagcg gctcctgaag gtccaatagc
     1321 gatagccccg acatttccga tcattccgat cactgccccg ccgatacctc caccgataca
     1381 agcagtaata aatgggcgtc ctagaggtaa tgttactccg taaatcaatg gttcgccgat
     1441 tcctaggatc ccgactggta aagctccttt gatcatttct gttaattctt tatctttttt
     1501 gcagcgaatc cataatgcca atgctgcacc cacttgtcct gcaccagcca tcgcaagaat
     1561 tggaagcaat agcgtacgac cagtttcatc aatcatttgc acatggattg gagtcaaaat
     1621 ttgatgcagt ccgaacataa ccatcggcaa gaataatgtg cctagaacga agccggcaaa
     1681 tgctccacca acgccaagta cccagttgat cccaccgacc atgccatcag aaatgaaacc
     1741 tgctaaaggc atgatgaaga aaatttcaat cagaccgatg gcaatcaatg aaagagttgg
     1801 tgtaacgatg atatctaccg catcaggtac tactttatgc agttttttct caacgattga
     1861 caatagccaa acagcgaaga tgacacctag gatcccacct tgtccagctg caagagcttc
     1921 accagtaaat aagtttttca gtggtgcttc cggattcatc cctgtcagca tcacaacggc
     1981 cccgataact ccaccaagag ttggtgttgc accaaaaact tgtgcagcat tgatacctgt
     2041 atagataact aggtagctaa acatcccgtt tttcaagatg ttcatcacat cgatatattg
     2101 ttgccatgtt gctgcatcaa gtgttccggc tgtgactaag tttgctaaaa ctgcggcaat
     2161 ccctgccacg atcccagtac cgacaaatgc aggaatcatt ggcacaaaga tattagaaat
     2221 gtcttttaat accgatttga ttttagatgg tttttgtttc gatttttgtg ccgctttcat
     2281 ttcttgtgcc cgttcgttta cacggtcttt tccagatgct gatgaatttg ctgtagctgt
     2341 acttacagaa gaaagttttt ctcctaattt cacgcctgcc aaatcaacca tttgctgtgc
     2401 tactttgttc actttcccag gtccgatgat gacttgaagc tgttcatctt ctactacacc
     2461 aagcactcct ggaatcgctt ttaattcaga gacatttaca agatcatcat tgatgatctt
     2521 catacgcaca cgagtcatgc agtggacaat gctttctaca ttgcccatgc cgcctgcccc
     2581 tttataaatt tgttttgcca gttcttccaa agtcagttcg ttttttgcca tggtcttttc
     2641 cccctcataa ggtccctttt acaaatccgt ttgcttcagc taattttgct tgagcttcct
     2701 ctttatcact atttgtcaaa atcatgacga tagccagctt aacattttga tctgcttctt
     2761 caaagtactt ttcagctgtt tcgtaatcag cttctgtcgc ttgcatgatg attcgttttg
     2821 agcgttcgat tagtttttca tttgttggtt tgacatcgac catcaagtta ttgaatactt
     2881 tacctatacc aatcatactg atagtagaaa tcatattcaa gataagtttt tgtgctgttc
     2941 ctgacttcaa tcgagtagag cctgtcaaaa attctggacc tgcatcgact tcgattggga
     3001 aatccgcgtg tttactgatt tcggcttgtt tattgcaaga aatcgttcct gtgacagcac
     3061 cgatcgattt tgcgtaatct aatccaccga tcacatatgg tgtacgaccg ctagctgcga
     3121 taccaataac tatatcattt tttgtcaaat tcagcttgat tagatcttct tgccctagct
     3181 cataagaatc ttcggcccct tcgacagcta cagtcattgc ttcttctcca ccagcaatta
     3241 gacctatgac catttccggt tctacaccga atgtcggtac gcattcagca gcatctaaca
     3301 cacctagtct tccacttgtt ccggctccca tataaatcag acgtccacct tttttaaagg
     3361 actcaatcgt gttagcaatc actggttcga tacttgtcag ttctttttca accgcagcgg
     3421 ccactttctg atcttctttg ttcattagtt gaacggcttg agaaatactc atctcatcta
     3481 atccaaaagt ttcttggttt ctgcgttccg ttgttaggtt ctctaaattg attttgctca
     3541 tttttatctt ccttcttttc ttataaattg atagttcata ccagctttga tttggttgat
     3601 caatggtagc tcttctttga taactttagc tactacattc actttttcat ccgctggcaa
     3661 atcatatttg gttaattgga tctcacccat atagcgcaaa tacttttcgt tatctaaagt
     3721 aatagaccct ttcatacgcg tagctgtatt tcttgctggg ataggcggaa tctctcgaaa
     3781 acgtgcatct acgcttcgta tgacatctct tgcatcgtct tgccgattcg tatgcttccc
     3841 taatatatat tcgtaaaatt cttcatctac aggttccgta tggagcaaga ctgtctgttc
     3901 tttttggaaa gaagcaaatt gctcctgaac gttttctgac agacctgcat ctccgatgta
     3961 aacaatatcc gtgttacttg ctaatagatc tagagcgcct gcaagaggat ggatccctcg
     4021 atgttcttcc aaagttggca atccttgata tagtgggcct ctcaactctg tatctcctgg
     4081 tacaaaacct tggacaagga gaccttgaat tttgagccat tgattttttt tctgatacca
     4141 ttctttgtcc agccctgttt cgggacgtgg ataataatta tgccaagctt caatctgtga
     4201 gaaatcagct tctgcttctt tcaattcatc gatatcttta ggcgtgatcg tgctagcatt
     4261 caaactgatt ttcaattgat gtgaccagtc ggcaatctgc tgattggaaa tatgataatc
     4321 cattcttaaa cctgttacac caattgcttt caaaggcgtt agatcttcta atgaaaaacc
     4381 cgctcgttcc aatgcctctc ctgaaatatc gaccatcaat tctagtttat tctttttcgc
     4441 ttcgcttcct agttcgatca atcgcttttg atatgccgaa acatcatctt ctggtatatg
     4501 catggaagtg aaaataccga caaagccatt ggatgccatt tggcggatat actgtttctt
     4561 ctcttctgac aaagaatcgt tcataaatat tgaaaagcca aacatattat ccctcttttt
     4621 ctgtatccgt cttcatttct atgactagag tatatatcta aagaaacaaa atttcaatac
     4681 ttttattaaa ataattaaat ttcatttcta aaggttgagt ttatattatc aaaagaattg
     4741 tcataaccta aaaaaggcac tgctaagcaa aagcagtgcc ttgaaatatt ttatttaaat
     4801 aacacaaaaa cataattact cataagaaac caattctgta ttatctacca tgaatgtaaa
     4861 gaacccctca ttaggcatcg gatgaaaata agcattgatc actcttatca aggcattatg
     4921 gcaaactaac agatagacat tttcttcgtc ttcgatgcat tctttcaata aaggaacgat
     4981 ccgtgcatac acatctaaga ctgactcccc atttgggaat cgtacagcga acgctaaacg
     5041 tgctttttga aagttctcat ctttgcttgg cagtccatca taatccccaa aatccatttc
     5101 taccaatcgt tcatcaacgg tcagcggcaa gctgagacga tcagcaactg cttgtgctgt
     5161 atctcttgct cgttgaagag gggaatggat gattttcgtg attggaacag gcaaatctac
     5221 taccttttct gctagtttct gtgcctgcat ctgtcctttt tcagtcagcg gcacatctgc
     5281 ccgtccgcaa actaatcctt gtgcgttcca agttgtttct ccgtgtctcg ttacataaag
     5341 catgtgattc tctccttcgt tggcaaagtt ccccttaacc ataccttgtt gttcttgaga
     5401 ttacaagggt tagattctct caagagaaat aatctcagta gatactttat ccaggaaggt
     5461 ttgatcatgt tccactagta acatggcagg tttcacgttc aagatcagtt gttctaattg
     5521 ttcttgattg aagacatcca aataattcaa tggttcatcc catgtatata gttcagctgg
     5581 ctgtgataaa gatttagcca attccacttt tttccgttgg cccatactca tctgctcgat
     5641 cttgttatga aaaacatctc tttccatccc aagctttcgg aggttgttca aaaatgcttg
     5701 gtagtctact tggtttttct ctgcaaattc cgccaacgtt cctcgattgt cttcataatt
     5761 ttggcttgca taactaatgc tcagatgttt tggctggtat ttttctccta taaccttgcc
     5821 gttgaatgcc cccagaagat aatggataat ggatgacttt cctgcaccgt taggacctga
     5881 aatcgccacc cgctgatgag gctcgattgt aaaatgaatt ggctcgaata acagattttc
     5941 ataccctaat tgaagatctt ctacgcttaa aagtcgctta tggtgagacg cttggctatt
     6001 catcgtcaaa gaatcgatat attcgatatc ttttaatagt ttttctttct cgctgatctg
     6061 ggtctccatc cgattcacga ttgctttgga tcgtttcatc gtccgtgcag catcagcacc
     6121 cactgctcct ttattcactc gtctagattc agtatcgatg aatccgactt gtttctttgt
     6181 tttatctcct tctcgggaac gagaccattc ggctttttca gctgctgttt tctttagcct
     6241 actgacttct tttttcaatt tttcattttg agccatttca aattcatcac gaagtttttt
     6301 ctgttcttca tagatagaga aattcccttg ataaagttcc agttgacttt tttcgattgc
     6361 taaaacatgg tccactactt catcgataaa tccccggtca tggctgacca cgatgaagcc
     6421 ttgttttttc tttttcaaat aagccgctac ttgttttcta ccagagatat ccaaatgatt
     6481 cgttggttca tcgattaacg ggaaatgagt gtcatccaca aataaaagtg ccagtaggac
     6541 ttttgtcttc tctccccccg atagtgtgct gaattctctc cataagattt caggatctgt
     6601 ttgcatcaat tggagctctc tttcgatttc ccatatctca aaatccgtaa tatcatttaa
     6661 cacgtaatag gttaaacgtt ctttatcttt tgtctgttgc gggaaatagg caaattcttg
     6721 ctgatggatt acttgccctt gataaggtag tttgttttgt agaatattca gtaaagttgt
     6781 ctttcctcga ccgtttcgtc cgataagtcc tagtttccat tgtgtgtcaa aatttagatt
     6841 tgcttgttca aataataatg tgccttggct gtcgtagccg aatgtcagat tttttatttc
     6901 gattttagac attttccatc acctcgtaaa aaaagaccag tcctattttc agaactggct
     6961 aaagaggatg caagggttgg atacacacaa aaagacccat acatcgaaaa aaccaagcca
     7021 atcccgaaaa tctgcacact gcttccttgt ctccacaagg tacagtctga atgcagcttt
     7081 catcaatcga cttaattttt caatgcacgg atccctcact ttgtcctata tttgaaatta
     7141 cgatagcata aagaaaatga ttttgtcaac tacttcggga ggatggtgaa aaatctgcta
     7201 agtgtctgtc ctaagatttt tgttttttct aatgtttgcc tgtgttccat tgcttcttct
     7261 aaacctattg gacccaaatg aatacttaag acaattggga gaaaatcaga aagtccttct
     7321 agctcttggt ctctacttcc gcaaatagcg attattggtt ttcgatattt ttttgcaagc
     7381 actgctacac cgtatggaac ttttccgtgg atcgtttgct gatcgatccg cccttctcct
     7441 gtaatgatga catctgcttg ttcgatcttt ttttccaatc cagtgatttg gctgactaaa
     7501 tcgaaaccag aaaccatctt cgctcccaac aaaaaaaggg ctccaccaat accgcctgct
     7561 gctccacttc ctggaatatc atctaaatcc acctcccaga aatcttttat ttgctgagca
     7621 atctttcctg cttgtttatc taagaaagct agcgtcgtat ctattcctcc tttttgtctt
     7681 ccaaattgcc aagcagctcc ttgttgacct gtatatggat tggtaacatc tgcagcgata
     7741 atcaatttta ctttagaaac aagaacgttt cttcgatcaa aatgaagttt tttagtagat
     7801 aataaaggat ttccttctgt gtacccttct aaagaagcac ccaaggattg gagtaatcct
     7861 agaccaccat cgctacaccc gcttcctcct aaagtgacaa taatttgttc tacttcttga
     7921 agcagtgcat ctttaataag ttctcctaag gcataactgg aagcaagtcg gatagtttga
     7981 tccgttggtt tatcaagtag atctaacccc aatgcttgtg cagattcgat gactgcggtg
     8041 ttttttcctt gccaagttgt caataaatag ctgatttttc gctgacggaa taagagatcc
     8101 atcccatcaa tggtacgcca tgttcccctg agcgcgtaat agattgcttc tgcagtattt
     8161 tctccgccat ctgcaatcgg cacattgatt tttcttcctt tacgatcttc ccagccattt
     8221 agaactgcct gatttagttc aaatgaagaa gcacttcctt taaatgaatc aattgccgcc
     8281 aaaatatcca tctgtatccc tcactttctt cttaagtaag acactttttt tcattttttt
     8341 caaacaaaac aatgctattt tcttccattt ttctatatac taaaagggaa ctcattgaat
     8401 aaaggagaga aaaaacatga aaatcgcaat agctggggcc ggagcaatgg gcagccgaat
     8461 cggattgatg ctacatcaaa gcggaaatga ggtcctcttg atcgatcgtt ggcctgctca
     8521 cattgaagcc attcgtacaa atgggttgat tgctgacttt aacggtaaag aagtggtcgc
     8581 aaaactgcca atctattcac cagaagaaat catcgaaagc aacgaacacg tggacttgat
     8641 cgtagcatta acaaaagcca atcaattaga tgatatgttt tgttcgatcc aatccattat
     8701 tactgacaat acttatgttt tgtgtttact aaatggatta ggtcatgaag atgtactgga
     8761 aaaatatgtg ccaaagaaaa atatcttatt tgggatcacg atgtggacag ctggtctagc
     8821 tggtcccgga aaagtcacat tgttaggtga tggagaaatc gaactagaga acttggaacc
     8881 agaaggcgaa gcatttacaa aaaaagttgt agaagtcttt caagaagcaa atttaaatcc
     8941 gatctatagc cataacgttc gttattccat ttggcgaaaa gcttgtgtaa atggaacatt
     9001 gaatggttta tgcacgattt tggattgtaa tattgctgaa ctaggcgcgc aaaaagcggc
     9061 agagagcatg gttcgaacaa tcgtatctga atttgcttct atcgcagcaa aagaaggaat
     9121 catactagat caagaagaag tgtaccaaca tattgcttct acttatgatc ctgacaacat
     9181 cgggttgcat tacccatcta tgtaccaaga cctgatcaaa aaccatcgtt tgactgaaat
     9241 cgactacatc aatggtgcca tttggcgaaa aggacaaaaa tatgatattg ctacaccata
     9301 ttgtgccttt ttgacccaat tagtccatgc aaaagaagga atccttggcg cagaataaaa
     9361 ggctctctga tgagaatcag ctgaatcagc agctggttct ctttttttaa gcccaataaa
     9421 agatcaaaaa gaaaacacaa ctctcatgat gccataagaa ttgtgcttgt ttgtttcagt
     9481 acataatctc ttcaaaatgt aggtttttta tttgttttca acagaggaga ggtcatattc
     9541 gtattgctca taaatagtgg aatcgttagt gataaatcgt tttgcccaat tttctgcatc
     9601 ataaaattcc aatagctctg ataggattgg cttaaatggt gtatgcagaa attccggtaa
     9661 ttcatctaaa tcacgttctc gaagcaaaaa aatattttca ggacgataaa aatgataatg
     9721 agcaatcgct tgattatccg tcactagttt cttttgaaaa tacatggatt caaatgtccg
     9781 caaagtcact ccttgctgac cctcccgcaa cagttccaga atcccgcgcg attcccctgt
     9841 ttttttcagg tactcttcat aagtaagata tccaggttgt tcattccctc gatgtggaag
     9901 aatgaaatag ttggtcgtaa catctctttt tataaattcc tgcttatagc tttctgctcg
     9961 ttcttttctc ccattattcg ttgcaccaaa aaatagatcc gtctttggtt cgctagaaga
    10021 aaaattcata tgtttcgaat aaaatgaact attatgtttc agcccaagtt cttttgcttc
    10081 caaaaaatca aaatgataaa tctcatcgat tgccggttca tcttgtgcct gcttcagcat
    10141 cgcctcgtgt tctgctttga agtgattcca aaaaaataac acaatttttg cattggtaaa
    10201 tgggcgcacg cgtcgagcca tctccatatc aaatccataa tctaaaaaaa cgatcagctg
    10261 ataatttgcc aactgttgtc tagctgattt aatgaattcc gactgtagta aatcttttcc
    10321 ggaccaaaca tctaaagagc ggatggaagg caaaaaataa agtgatttat tttttgcaac
    10381 tagcagtacg tcatttttgt tcatcttttc cgcctcactt tctctgtcac tttttcctat
    10441 gatagcctgt ttaaatagta aaaagccagt aaaacgtttc ctatatttgt atgaaaattt
    10501 aacatttgta tgaaaatatg atgaaataaa tcattcttct attaaaattc gcctaaaagt
    10561 tttctgaata gttgtaattg tctgaaaatt tgttatcatt agtcattgtt aaatattgct
    10621 atttattctt aatataaatt ttgattgtga atttaaaaga aagaggtcat ttatggaaga
    10681 taaaaaattg aaaaaacaac ttagctccag acatatcacc atgttagctt taggtggagc
    10741 aatcggtgca ggtctattta aaggaagtgg cgaagcgatt gggatcgccg gcccttctgt
    10801 attgattgct tttttgattg gtgggctgat tttatatatc gtgatgaaag gtctgggaaa
    10861 aatcgtttta agcggtggcg acacgcatca cggattatct ggtttgatac gcccttatct
    10921 aggtgctcat tctgctgatt ttactgattg ggtctactgg tctatgtgga ttatcaatat
    10981 catcgctgaa gctgttgcag ctgcttcctt tttgcagttg tggttcccgc acattccagc
    11041 ctgggtattt gtttttatgt tggctgtttt aactacgatc atcaatcttt attcggtacg
    11101 gttattcgca gaaacagaat attggctggc ttttgctaaa atcagtgtca ttatcttact
    11161 gatcattttt gctacttatc ttgtcggtca gcaaatgttg ggcacaggcg ttttccctac
    11221 ccttcagcaa ttgactgacc atggtgggtt tactcctcat ggtatgaagg gaatcatcag
    11281 ttctttgctt gtagttatct attcttacgg cggttctgaa ctgattgcta tcacggtaag
    11341 cgaagcagat gatcctaaac gtgcgattcc taaagcaatc aaaggtgtca tgggaagaat
    11401 catttccttt tacatcattc ctttgttttt gcttttgatt ctttttcctt ggaatacttt
    11461 agcaggcacg aatgtcagtc cttttgtgat ggtatttgaa aaaatgaata tcccatttgc
    11521 agcggatatc gtcaattttg tgattgtcct tgctttgttc tcatctatca attcgggtgt
    11581 gtatgcatct tcaagaattt tgttcttccg tttgaaggat cgaaaaggtt cctctagaaa
    11641 actagctgtt ttaaacaagc atcaagtacc gcaacgtgct gttttatttt gtaccagtgt
    11701 tctttatctt ggtgtagctc tttcttattt tgtcggtgac aaattatttg gctatctggc
    11761 aggttccctt tcgtatacag tattattgat ctggatcatt atcagtgcgg catcctttgt
    11821 actggcacta agaaaaggaa cattatggga taaaggaatc agcttttttg cattagctgt
    11881 cttaggattg attttccttg gtatcctgtt cacgaactcc attggggtaa ctgtattgac
    11941 tggcctgctt tacctgttca tttatttcag ttatcaaaaa aagaatgatg cgttcgtatt
    12001 gacgaatgaa tgaaatcgat agaaaatttt attttattta tgtggaatag atggagctat
    12061 tgaagaaagg agttgtgaca aaagtcttgt tgcaactcct ttttccgaat aaacagtctc
    12121 atgacctctt ggtaaacggt gttcaaaaac cctctgtgca aaataattga ttgttctcca
    12181 tgctcatgac atgatagaag aaaggaatga aaaaaaggag tttgttttaa atgaaaaagt
    12241 attggaagtt tttgttgatg atcggtgttt caacggtagt tatgtttggt atgatgtact
    12301 tgaatgtcta tgcgctcgat catatcttct tcagtcgtac acgagtattc atggcgttga
    12361 tgatgggtgc agtcatggca gtcatcatgc tgttgttcat gtggaaaatg tatgaaaaca
    12421 aaaagataaa tgttatgatc ttagccgcta gtcttcttgt ttttgccggt tccttgttta
    12481 tggtacgaag ccaaactact gtcaacgaca cagcttggat gaaggccatg attcctcatc
    12541 attctatcgc tatcctggca agtaaacgag ctgatttaaa agatcctgaa gtaaaaaaat
    12601 tagcggaaga aatcattcag gcacaagaag aagagatcga caaaatgaaa aaaatgatca
    12661 aagaaatgga atgaccatta tccgtccaaa taaaaaaaga ggatgttcca gaagttatca
    12721 gcttcaagaa ataagaagaa gctacttctg gaacgccgtg aataaggggt tgtgaaaaag
    12781 ctgccctgca tcaagtaata agaacagcca aacaaaaata gcttctccta tttttcgttt
    12841 ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattaaga ggttgtaaaa
    12901 tcagcgttct gacctgagta ttagagagac aaatggaaaa atagttcctc atattttgcc
    12961 attttttgag ctaataccga gggtcagctg attgaacacc gtttatcgag aggttgtgaa
    13021 aaagtcgctt agtttcgcgt attaagaaaa attttgtgaa atggctttcc acatttttgt
    13081 ataaatttgg cttaataccg agaaactggc ttttgaatac cgtttattcg taaaagggat
    13141 tgtgacaaaa gtcttgtcac aatccctttt tatgttttta ttgttctaat tgcgctgtct
    13201 tgcttactgc ttcttgcggt ttcacaaatt tttttgagat tccccaacct gtgaaggcac
    13261 tgaccatcga aaacacagga gaaagcaaac tgaaaaatac gaatggcaag taagtgagtg
    13321 taggaatacc aaatgtactt gctacgaaac ttccggcaat tccccaaggc actaagtagt
    13381 tgattactgt tccaccatct tctagcgttc tacctaatac agttggatca aatcctgctt
    13441 tttcataagt ttctttaaat gcattaccag gcagaataac agataggaat tgttcaccaa
    13501 taaaaatatt tactccgata cttgtcatca atgttgtaaa aatcaaattt ggtgtgttcg
    13561 tcatctttct tgatactcgt tccatcacgg ttgagatcaa gccaaattcg atcaacaatc
    13621 cacctaatga caaagttaga ataatcaatg acacagtagg catcatactt tcgattcctc
    13681 cgcggctaag cagtgaatcg atttgttggt ttccagtatt tgaaacaaac cctgattcga
    13741 tgaccccagc caacttcgtt aaggaagttt gtgggttttg aataaaaatc attcctactg
    13801 ctacagcgat attcaacaaa atcgttaaaa ttgccggcac ctttttccat gcacacagta
    13861 acattaaaaa taaaggaatc agtgaccaga ttgaaatagt aaagtttgct tctagaacat
    13921 cagtcacttc acgaatagat gaaagacttg cggctttact tgtgtgtcct aaaatcatga
    13981 ataaaaagaa agaaacaaca aatgccggga cagttgacca catcatatgc ttgatgtgtt
    14041 tgaacaaatc ggcatcaaca atagctgctg ctagatttgt tgattcggat aatggagaca
    14101 tcttatctcc aaaaacggca cctgaaacaa tagctccaac aaccagcgct ggatttaagc
    14161 ctagtgttgt cccaataccg aaaaatgcaa tcccaatcgt tgacataaca gtaaaggcac
    14221 tccctacagc gctacctacg attgcacaca caacaaatac agaagggaca aaccactgga
    14281 cactgatcaa tttgaaccca atgaccatta gagttggaat gatccctgct tgtatccata
    14341 cagcaatcaa tgaaccgact aaaataaaaa taaagattgg gatgattcct ggtttgatgc
    14401 cattgatgat tccttcgtgc agccaatctg tcgggtatct ttttatcact gcatacaaca
    14461 tgaccattcc gatcgctgct aagatcgtga tattaggtga gacctttaat ccgacaacgc
    14521 caatcgcaat gcctaaaaca ataaccatta atacaagtgt tgcttctaaa aaacttggtg
    14581 ttcctttttt tcccatgata atctctcctt ttttagttgt tcttatgttc taaaaaagtt
    14641 aagatatcgg attactccgc cgcaattcat ccgcgattcc tcctttataa aaataaaaag
    14701 acctgtttta aactaaggac gaatgactcc gcggtaccac cttaattaaa aacagatctg
    14761 tttttcactt tcaaactttt attcaaatgt atggactttc catgtaattc gtcaattagg
    14821 cttgatcggt tcacagcgac caccgacttt ctgaacaaaa cacctaaccg ctactgcgtg
    14881 aaaagttgac ttgtacgaag tttaccttcc ataagatcat ttgtcaatga aaaagaaaat
    14941 attttttcat ttttctgaaa attattttta taactttcac acacaagttt attttttact
    15001 agaaagtagc gccctcttgt ttttttcttg ctactatttc ttgatgttct tgcttgattc
    15061 tttctaatag ctttggctct ttcaacagat caagagctgt attggcgagt agttctgctg
    15121 caatcgcaat agaagcaagg cctttgtcac ttttagcagc agctttgaat tcttctgtgt
    15181 gtccggcaat atatgtctca gagattgcta ccgtaggctg gattgttggt atgacttggc
    15241 tgacatttcc tacatcagaa gaaccgagac ttgttctttg ttccgtttct atttcttcat
    15301 ctggaacacc tgcttctttt gcatgctgga agaataagtc atcaaaagaa ggagtaacga
    15361 tgacatcatc aactgcattt tggaacaatc caaactcaaa agtcgtgccc gtacttatcg
    15421 cagcacctcg tacgatattt tccacttttt catatacttt atctaatgtt tgccgatttc
    15481 ctgcacgtaa atagaaacgt ccagcagcgt aatctggaat gacgttggca gctactcctc
    15541 cgtctgtaat gatcccatgg atattgacat cttttggcaa ttgcaagcgt agagcgttga
    15601 ttccattaaa cacttggatc aatgcttcca aagcatttac tcctttttca ggtgctgctg
    15661 ctgcatggga agcgacaccg taaaatttga tgtcgactgg atcattcgct aaagattctg
    15721 tcgttttccc ataacgaaaa gctggatgga cgcataacgc tacatcgaca tcctcaaaaa
    15781 aaccttctcg aacaaagctg cctttagctg atccgttctc gcccccttct tcccctgggg
    15841 tgccatatac acggatttct ccaccagttt catcaatgaa ttgcttcact atgctggcgg
    15901 ctaatacgga gtaagtacca aacaaattat gcccgcatgc atgaccaata cctggtaaag
    15961 catcgaattc tgccaaaaag gcaattgtgg gaccgggttt attggaaata taacgtgcat
    16021 caaaggctgt tcggtgtcca gctacttctt tttctaccca aaacctttct ttttctaatt
    16081 gtttaatcaa cgctgctgag gaataaaact catagttact tacttcagga tgttcgtgga
    16141 tatctagagc aagtgcttga tattctggta aacgatattg gattagatcc ttcaattggt
    16201 ttgttgagat tgttgttgtc ataaaaaatt cctcctatgc tagtcatttt tcttattttc
    16261 tcccgaatgt ttcccatgca ggcacgcttg atccttttga tgttttctcg attactttga
    16321 ctgtgtcttc tgtttgataa gcgtctacta ttttttgata cgttttattg ttttcatctt
    16381 cttttcttgc aacgatgata ttcacataag gacgtgcatg gtcatccact ggttctaaga
    16441 agatggcatc agaagcagga ttaaatccag catctacagc tacaccgcta ttgatcactg
    16501 aaatgtctac atctgacaaa gcacgtgctg tttgggaagc atctaattca gaaatgtcta
    16561 gattttttct attctcggta atatcagaaa cagtgggcgc ttgtcctttt gccttatcca
    16621 ctttgatcaa tccagcagtt tgcaataaca gaagagctct gccgccattt gtaacatcat
    16681 tagggattgc tacttttgct ccgtctttga tatcagctac atccttcaaa gtgttagaat
    16741 aaattcccag cggtgcattg acagtatttc cgatcgatac taaatcagtt ccgtgttctt
    16801 tattgtaatt gtctaaaaag aattgatgtt ggaaagaatt caaatcaatc gatccatctg
    16861 ctaaagccac atttggttga ttgtaatctg taaatttcac gtattccaaa ttaatgttct
    16921 cctttttcaa acggtctttc acgttgtccc atacatctgt atcttctcca ataatcccta
    16981 gttttacgat agtttctttt ttgtcagttg tatctgcttt tccttggcta caccctgcca
    17041 accaaataat tgctacaaat gtaatgacta caaaacgaat aatttttttc attttttctc
    17101 ctccactttc aatgtgtaat ttttttaatc agttgattac tgataaattg acttaaaaaa
    17161 actaagatta gaatcaataa tgtagccaca atcgttacat ctgtttggaa tcgattatac
    17221 cctctcgata tagcaagatt ccctaatccg cctgcaccta ctgctccagc catggtagtc
    17281 agtccgatca cgttgatgat agtcaacgcc gagacccgaa tgataccagg caaaccttct
    17341 aacaaataca cacgtaaaat gataccgatt ggactggttc ccatcgcttc tgccgcttcg
    17401 atcaccccag gatcaacttc tagcagtgcg ttctcgattt gacgagcaaa aaacggaaca
    17461 actccaaaaa tcaatggcac taaagctgct ttcgcaccaa tcgtcgttcc aactaagaaa
    17521 cgagtagtca atgctaaaag agaaagcaag ataatgaaag ggatggaacg aatgatattg
    17581 atcagcttat caagtatttc aaatagcaga cggttagcta gtaatccatt agggcgtgtc
    17641 actactaacg tcacacctaa gatgattcca atcatcccgc caatgaatgc tgtccaaaaa
    17701 gacatatata gtgtttgctt cgtcgcttcg acaaattcat ccggaatcaa ataaacattg
    17761 ggcaaatatg ttttaaagaa atcagtcatt ttatcttcct ccttcgatca ctttgatacg
    17821 actatctaaa tcgttttttt gtaaaatatt tatttttaca ccagatttga tcaaataatc
    17881 caatgctttt ttccgctgtt tggtttctcc agctaaagta acgatcaaac tccctaacgg
    17941 cgtttcttgt aataactcta cattgccata taaaatattc gctgttactt gatatttgct
    18001 gtacagatga gcgattagag gttcgttcgt ctgatttcca atataactta attccaccag
    18061 ccatttattg gcgatctgat cggcaaaagc agaatgggca atgatcgttt ctaatgcttg
    18121 gtcaagatga gtagctgttc gaataaaatc tttggtcaat tcttcttcag gattgctgaa
    18181 gatctggatg ctgctacctt tttcaacgat tcttccatct tccataaccg ctaccttgtt
    18241 gcagatttct ttgacaactt gcatctcatg agtgatcagg acgattgtta aacctaactg
    18301 gcgattcaat ttttttaaca atgcaaggat ctgaagggtc gttttcggat caagtgcact
    18361 ggttgcttca tcacataata ataccttagg ttcattcgcc aaagctcgtg cgattgctac
    18421 tcgttgtttt tgtccccccg acagttgttt aggataagca gatgcttttt cttccaaacc
    18481 gacaagttcc agtagttcat tcactttttg acgacgttcc tgtttcgatt ttccagaata
    18541 ctttaaagaa aaatcaacgt tgtcaaaaat agttcggctt tccattagat tgaaatgctg
    18601 aaagatcata ccgatggcct ttctttcttt tcgcaattct ttgggagaga gctgactcag
    18661 attcgtctga ttgacaatga cttctcctgc tgttggtttc tgcagcaaat tcatcacacg
    18721 aactaacgta cttttccctg cacctgagta accgacgatt ccataaacat cgcctttttc
    18781 aatcgttaaa tctacagaat ctactgcctg tatctctttt tctttttgtt tgaaacatac
    18841 agaaacatct cgcaattcaa tcattaaaat tcctccttat ctaaaaaaag cgtcctcaag
    18901 aaaatgaaaa tgtcattttc ttgaggacgc ttttttgcgt gttaccacct catgttcaag
    18961 ctgtgttcac acacaactcc tcttcaagta cgatcatact ctagcaagat atcggtcgca
    19021 gccgtcatcg ccttacatct atgctcggga tgaattgctc gatggccatt ttccaatagt
    19081 cgttccttgc ttcttctcag ctaccgaagc tctctgtcaa aggttcttct atcgtactta
    19141 ccatttcaat gcaaataata ttcaaaaaaa aagtcctttt actaacataa actatgtcag
    19201 taaaaggacg acgtatcgcg gtaccacctt ttttcggaag tttccttccc tcaagcccaa
    19261 catctcaaaa agagagattg gcgttcgttt taacggtcga acagttccga atttgcctac
    19321 ttatcgacaa attattctcc aaggccattt tcagcttttc ttacttgact cttctcacca
    19381 gccagagcct ctctgcaaag tttccaaact tactttcctt ttcaacgaaa tcgatatttt
    19441 gttgttggga ttaatctaca tgaggaaaac tgaaaagtca agtacgaaat tcaaattatt
    19501 ttttctatat ttatttttct gtttttttat ttaacatttc aataacagtt cgaagatctt
    19561 ttccttccgc aagaagtttc aatttttcct tttcttcgac ggtataattt cggacaaaac
    19621 tggaatcagg tatcaaaaca acgcctaatc ctgctcgtga agcagctgtt gctcctacta
    19681 aagaatcttc cactgcaaag gctgaactag cttctaatcc tgttcgttcc aaagctgtca
    19741 gataaggaat cggtgaaggc ttgttctccg acacttcgtc tccgaatgta aacgtgtcca
    19801 agtaatcagc taatttaaag tgatccaaga tatctgttgc tcttttcgta acagtggtcg
    19861 tagcaatgcc tagtttcata tgacgttctt ttaagtctgc caatatttct tttgcatatg
    19921 gttttaagtg gatggctccg cttgtcagct gttgataaaa atattcttca cgcttcgctc
    19981 ggacttcctg tactttttca gggctacctg ctgctttcac taaaatatca aaactttgtt
    20041 tccagttttt tccgccccaa gcgtcaacga tttcttttgg gatatggata tcaaattgct
    20101 gaaatcccca aagccagcca tcacgatacg ttttttctgt gtcaactaac aaaccatcta
    20161 aatcaaatat tgcacctttc atctatacat taatcctttc caaaacaata cacatagttt
    20221 agcacgactg gtccaaaaag aacagaagca aagaaccagc cttctcgttt ataagagttc
    20281 ttggaatcga cggatataga ttttcttcac agctgtcaca agcgccagat aagcgatgac
    20341 tgtagcaaat aaccataacc agtaattgcc aggaagtggc atcaagccta attcagcacc
    20401 aaaacttgta aaaggaagga cagaaccgac aataattcct aatgtagtga taacaaacat
    20461 ggcaaatgtt gcgttgcttt ggataaatgg tactttcggt gtacgcagtg catggagcac
    20521 taaggtttgt gtccatagtg attcgacaaa ccatcctgca tggaataatg cgatgaaaat
    20581 cactcgctgt gaactatcca atgtatggaa acttccgccc actacagcag gacaaataac
    20641 aaaatacatc aacaagtaag tagtaatatc aaatatcgaa cttgttggac caaagtaaac
    20701 catgaattcc ccaatactag atgcattcca tttttttggc tcgtccaaat attctttatc
    20761 catccgatcc catggcaagg aaatgcagga aacatcatag atcagattca aaaacaaaag
    20821 ttgtaaaggc agcattggta aaaatggcag gaacgtactt gcaatcagca cggaaaacat
    20881 attgccaaaa ttagaacttg ctgtagcctt gatatatttc atgatattcc caaaagtttc
    20941 tcgtccagaa agaaggcctc tttccaaaat cgtcaaatct ttttctaaaa ggatcacgtc
    21001 tgcagattct ttcgcaatat ccacagctgt atctacagaa ataccgatat cagccacttt
    21061 cattgcagga gcgtcattga taccatctcc taaaaatccg acagtatgac catttttacg
    21121 taaagctgtc gtcaaacgtg ctttttgctg aggattcaat tttacgaata cttcatggtc
    21181 ttcagctact tgtgctaatt catgatcact catttccgag attttctcac cggtaaccaa
    21241 ttcattgatc tctagaccta cttgacggca cactgaacgt gtaactaatt cgttgtcccc
    21301 agtcagaact tttacagcta caccatgttc tttcagagct ttcaacgctt gtttcgttgt
    21361 ttcttttggt ggatcaagga aagcaagata accaatcaaa accatgtctg actcatcttt
    21421 gactgaaaat tctccaacga cgcttggatt ggtcttttgt gcgacaccaa tcactcgtaa
    21481 gccatcttca ttaagtgact gtacttttgc aagtacggct tcttttcgtt cttttgttaa
    21541 tggtgtaaca gtaccgccaa gatcaataaa tttagaaatg ttcagcattt cttcaatcgc
    21601 gccttttgtg atcatttgtg ttttaccagc tggatcagca accacaacac tcatacgacg
    21661 acgttcaaaa tcaaaaggaa tttcatccac tttttgataa ttgatcttat ctgtatctaa
    21721 agtttctgtt gcttcatcaa tgatagccac atctaacaaa tttttcagcc ctgtttgata
    21781 gtagctattt agatacgcat ggcgcaaaac acgatcatct tcatttcctt cacaatccat
    21841 atgatattca agaatgattt tatcttgtgt caatgtcccc gttttatccg tacataacac
    21901 atcgattgca ccaaagttct ggatggcatt caaatttttg atcactgttc cttttttggc
    21961 catcacactt gctcctttga ccaaattcgt cgtaacgatc attggaagca tttcaggtgt
    22021 tagaccaact gctacagata gtccaaaaag aaaagcttct agccaatccc cttttgtaag
    22081 cccattgatg acgataactg ctggagccat caatgacata aatttgatca gtagcatgga
    22141 tgttttatgg atgcctaatt caaagcttga ttgaattggt ttttcagata gcgtttctgc
    22201 tacatgacca aatagtgttt catttccaac agaaacaatg attccttccg cacttccact
    22261 gatgacatta cttcccataa agacaagatt ttcgtaactt gtttcgcttt gctgatcgtc
    22321 ttcacatact ttcgcatgct tttctactgg atagctttcc cctgtcaatg ctgcttggga
    22381 aatgaataaa tcctttgcgg acaataaacg catatccgct ggtatcatat cccctgctga
    22441 aagccgaaca agatctccac agacgatttc ttccatagga atctctgtgt actttccatt
    22501 acgttttaca gctgccgtta ctttcacaag tgatttcagt ttttcagccg cttgattgga
    22561 acgtacggac tgaatcaacg tcattgtccc gctgacgata accatagtga agataatggc
    22621 tacaccgaaa agatctttat ctccaggagc tgctaataca tattccgtga tgaatgaaat
    22681 aattcctaat gcgatcaaca ctaatgtaaa tggcgtgatg tatgctttca atatttctac
    22741 aatgaatggt gtttttttgc catatgaaat cgcattttca ccgtaatttt ctcttgaacg
    22801 ttcgatttgt tcttctgtca atccatttgc tgatgcatgg aatagttcaa ataattcttg
    22861 tttcgttttt ccagcaaaag ctgcatagat tttttgtgta tctttctttt tttccatctc
    22921 aatctctcct ttgtgtccaa aagacagctg ttctcgctgc ttttggtctt tagctattca
    22981 tcggatcgat agagaaaaaa ctgaaagggg ctgagaacag atagataata gccctggcct
    23041 ttcctctaga tccttgtttt tcgaatgact gtcatccatt tgttctcatc tcctttacta
    23101 agtaatttta attcatgact tccaatcatt tttcgaatag ttatttttcc aattagacca
    23161 cctccttttt atttttcacc gatggggatt tgcggcaaca aaaaaacctc tcgccaaata
    23221 ctcagcgaaa ggtttgaaaa agcatattaa aaaaagcttc tcagatctca tcgttgagtt
    23281 ttgacactgt acaacgtaaa aagcagatac ctgcttttag ctaccttatg aaaagcctta
    23341 tctcgacaat tcctgttcta cccattggcg tctctcgacg tttttgggca gtagcctatg
    23401 tttgtatagg agcctcacct aacaaatctt attcggtttt ccttcgttaa tataacaact
    23461 aaaaaagagg atgtcaaatc aattttccat gtttttcata ctttttttaa aatacacacg
    23521 ttatattcct gtctcacgcg gttttatttg tctcattttc cacctcccgc tataatagaa
    23581 atatagtagg aaacgaggtg ctgttatgga aagaacactt gttattatca agccagacgg
    23641 cgtgagacgc catttagtcg gaagcatcat ccaacgtttt gaagcaaagg gactggccat
    23701 tgctgaaatg aagtttgaaa ctatgactcc tgaattagca aaagaacatt atcagcactt
    23761 gactgagcgc agtttttttg atgaactgat cgattacatg acttccggtc ctgtcgttta
    23821 tcttgtgctg gtcggcgagg aagtgatcga tatcgtccgt aagatggttg gagcaacaaa
    23881 agctgccgat gctgttccag gtacgatccg aggagactac gcgctgccgg ggactgaaaa
    23941 catcattcac gcttctgatt cccgtgatgc tgcggtaaaa gagatcgccc gctttttccc
    24001 gccattcgat ataaacaaac cgtctgaaaa agcggcgaag tgatacaaat tctaaactgc
    24061 tggtctagtt gctgggttca gcaatggcca gcagtttttt ctctaaggac ctccgttgat
    24121 tctttcctaa catatattcg agcactgtga agaattcttg gtagtctttc gtcttttctt
    24181 cccctaaagc agcagaaatc aatctttgat aataatctgc catcaatgtt tcatagatca
    24241 ctgtacttct ttctttatgt gataagctcc gtttcatatc ttcattcaca ctacgtgcct
    24301 ttttcaaccg accattttct atataaaacg aagtcaggtt cattaaaaaa gtaaagagat
    24361 actgctcatg attgttcaac tgtgctaatc gtcgcattct ttttttgatt ttcaaataat
    24421 tgttctcaat gaaccgatca ctgaatatcc aaggaaaatc agctagaata gatagttcaa
    24481 attgaccata gtctggtatc tgcatcaagt attcttcaat gattcggcat tcataacgta
    24541 aaaattcaaa ttctgtctca gatacttctg ttttttcgac catgtgaaga ttcaaaacca
    24601 aataatacag atcttctgtt ttcttaaaat gcctacggtt attgctgatc tctttataag
    24661 gatcaaagct gtcttcttgt acccgatcta gcattcgtct ttttttcttt aactttattg
    24721 gttcatccag ctggtttcgc atccattcat attctgcaag tgtcatgttc attcgctcta
    24781 aaaatgatct caatgttaca aatgacattt ttttcactga atttgtctca atcccgacta
    24841 gagaagtctt aaaacagatt ccttccgcta attgggactg tgtcaacttc ttgttgactc
    24901 gaagtttctt gatcaattca ccatcataca taatagttct cctttttaat ttaaattttt
    24961 ccacattcaa taaatagata cgttaaaaag gaaaaataca cgaaaaaaat aaaaaaatgt
    25021 ttttttcata aaaaatacca accttcaagc attagaatgt tgtctaacta ctgaaggttg
    25081 gtttgattgt ttattgatta tttttttgct gttcaacgtc tgttcttgtt actggatata
    25141 agttggttgt tgttttatta ccattttttg aaaagacact tgtcgaatgt tcgccttttt
    25201 gcttttcgat tgcttcatta cgttccgctt catttttata gtcatatttt ttcggatcaa
    25261 tcgtctcaaa tccgtttggc acataaaacc gcaagagatt ttcctgattc agcttatctg
    25321 aaagacttaa cgcagtcttg actttttcct gatctttgga gatttcctca tcgattcctg
    25381 ctgctttcgt atcgatgatt tctcctgtct gattattgta agcttttcct cctaggacgg
    25441 tgtatttggg tgtcacaaag ttcccatttc gaaaagcaac tacttgatca tgctgagaag
    25501 ataataagtc tgtaccaaaa tgaagataat ctttatcatc gattcccaat agatgtagta
    25561 atgtcggcaa aacatcgatt tctccaccgt attcatgatt gactgtccct ttagtataac
    25621 caggcatatg gatcatcaat ggcactcgtt gcatctgtgc gttgtcaaat tcatcccatg
    25681 tatctggatc tttaccaaga gcgcttgcta aatctttatt atctgtattc gagatcccaa
    25741 aatgatcccc gtaaatcaca aagattgaat tttggtaaat accagatttt ttcaaatacg
    25801 taaaaaattg ttccaatgat tgatccagat aatgtgccgt tcgaacataa ttatctactg
    25861 tgctattgcc agtattcaaa gatggaaagt cgaaattctt gtcatccgta taataaggcg
    25921 tgtggttggt tacggaaaga aatttcgtgt aaaacggctg ttgtagatgc tcaagatatt
    25981 gggcagattc tctgaataaa tctttatcta atatcccata acctaacgtt tcgtcggatt
    26041 catcaaaata agatcgatca aagaaattgt cataacctaa gtttttatat acatgatctc
    26101 tattccaaaa actagctaca ttcccatgga aaacagcgct tgtatagcct tgcttttgtc
    26161 caaggatcgc tggtgcggct tgaaagacat tgtctgaccc aagttctgtg aataatgagc
    26221 cttgaggtaa accaaatgtg ccagtttcca acatattttc agcatcactt gtctttccct
    26281 gtcctacttc atgaaagaaa ttgtcaaagc tgatcgttgc ttgattctgg aaaatactat
    26341 ttaaaaacgg tgtgacttct tgtccgtcta ccttcatatt aatcaaaaac tgctggaaac
    26401 tttctaaatg gagaacgatc acatttttcc ctttagcgat cccaaatgtt tcgggatttg
    26461 gctctgcata atgtttcttt gtataatcta aaacttcatc aatcccatta ctgcttgcat
    26521 gcgcgcgaac actgctagtc attcctgttt tgattccgtc ataaatagta tacgcatcga
    26581 ttcctagata tttgacaata tatgaacggt caaatgttcg ctgcagcaat tgtggacggt
    26641 tggcttcgct cagcgctaga tttcctgaaa aaataagccc tgaaagacag gtaacagcga
    26701 tagccatcgg tttgccgaca gggttgcttt tgataggtat tttcttgacc ttcaaccaaa
    26761 ccagtagccc aatgaacaca gcgatatcca gccagtagat gacatcatgg ggcttcatca
    26821 aagaaaagga gcttcctgac aatccttgcg acactttcga aaagcctaat acagatttga
    26881 ctgtaatgaa atctgtgaat tcccgataaa aaataatatt caagtacaac aaaagcgtat
    26941 ttaaaatatc aatgatcaaa agaatagcta acgctggctt tggcttttta atatacaaag
    27001 aaaggccaaa aaacaataag gtagtcgcaa ttgggttgat ccacaagata aagtactgga
    27061 tcgtaccttc cacacctaat gaaaaatctg catagtaggc aatgatcgtc ttcagccaga
    27121 aaaagaaaac agctgtcaaa acgattgcta aacgattttg gcacaatttt ttaagttgtg
    27181 tcacgtatat cacttctctc tattcacatt ttttcttatt ttgctcctga aaaatgaaaa
    27241 tttcctaaaa aagttgttat cttaagattt ttttattctt tctaaagatt attccgtcca
    27301 cttttcacag cataaaaccg ctgcgatgta agcgattaaa ctaatgaaga gataaaagtc
    27361 ttgtggtgac cgcttcactc cacggtattc aaaagccagt ttctcggtat tcgtacaaaa
    27421 aaagaggctg tgacaaaatt cttgtcgcag ctccttattt cttgatgcag gacagctttt
    27481 tcacaacttc ttccttaaac ggtgttctcc tgccaactcc tcggccacaa gtcacaatat
    27541 ttataaaaca tgagaagctg ttttttgaaa ttgttccttg tggctcagag ttaaacggca
    27601 ggctcaagac ctcttgatac acggtgttca atcagctgac cctcggtatt agattatgtc
    27661 aggacgctaa ttttacaacc tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat
    27721 aagcccaacc aaacgaaaaa tataaggagc tattttttga aattgctcct tgtggctcag
    27781 agttaaacgg caggctcaag acctcttgat acacggtgtt caatcagctg accttcggta
    27841 ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc gtttgtttct ctaatactca
    27901 ggtcaaaacg ctgatttcac aacctctgtt tttcattgtc cataggattg atattgtcct
    27961 tgtgatgact cattcgtaat aattgaggct tgattttctt cacttcccgt tatcgtttgt
    28021 ccactgccag aatctgaagg acttgttcct gtcgactcgt ttttttgaga agagacagtt
    28081 gttttcttga cttgttcatt tgtctttttc cattccgcta aacgatcggc gaccactttt
    28141 ttgtctactt tttcagaata agcggtgatc acttttccat ttttcaaacg ggctactttg
    28201 tcgccaacat gagtcaaaag caaaacggtc aaatgcttgt ctttaaattg atattgtctg
    28261 agagaaatcg cactaagtac gtcatcgata ttaataaatt gattttcttc ttttcctttt
    28321 ggcaaataaa tgtccaattc gcctttttcg taattggcag ttattttata ctccgcccac
    28381 caataagttg agaattcttt tttgaaatta tctactgatt ctttgcttgc tacgattttt
    28441 tctgtttctt tctgagcaag atctgctgtc atttttcctg aaaatgctgc ggataaaata
    28501 agcagtaaga aaaaaccgaa taaccaaaag agaagaaacg gtcttttttt cctcatgctt
    28561 tcctccactc ctatttaatt ttgactattc tattatatac aaaaaatgaa atagaaagac
    28621 aaaaaagttg aaaaagatcg taaataattg aacttctttt tcacaaaatg gtaagaatgg
    28681 agaatttcac gcatttgctc tcaactattc tttcttaagc ctcaaatccg tttttatctg
    28741 atacgtcttt gaaccaatga cccgattttt tgattgttct ttttccatct tgtgctaagt
    28801 ctactgaaat aaagccatat cgattcttat atgcattggt ccacgaccag ttatccatac
    28861 atgtccacat atgatagcct aaacaattgg caccttcttg aatggcttga tggacatatt
    28921 tcagatgaga ttggacaaat tcgatccgat aatcatcgtg gatcatgcca tcttctttga
    28981 taaatcgtgc ttcgttttct acacccatac cgttttcaga aataaagcag tcgatatttc
    29041 cataatgatc tcgtacattg atcaaaatat catagattcc ttgttcatag atctcccatc
    29101 cgcgataagg attcatttta cgaccaggca tttcataatt gtcgaaataa tcctcaggca
    29161 ttggaccgtt tttagtatcg attggtgttt cttttgcttt tacccggcgt ggctggtaat
    29221 aattgatacc taaaagatct actgtatttt ctttgataac cgtcaaatcc tctgcatgga
    29281 tcacaggcat gagatcaagt tcttttacta tctcaatcaa ttcacttgga aactcgcctt
    29341 ttacagctgg atcaaggaat gaacgattga agaacgcatc tgctatttgc gctgctttaa
    29401 catcttcagg gtttgattcg tctcttggat agcttggtgt cagattcaag atgataccaa
    29461 taacaccacc ttgctccatt tcatggtaag atttgatggc taacgctgaa gccaacgctt
    29521 catgatagcc aacttggatc gcttttttca tatctgactc ttctggataa tgccatcgat
    29581 ataaataacc tgcttcgact ggaacgattg gttcattatg agtaaaccat ttttttactt
    29641 tcgaaccaaa caattcaaaa caacttttgg catatttttc ataggcttca accgtttctc
    29701 ggttcaacca tccgcctttt tcttgcagat acatcggcat atcgaaatga tacagattca
    29761 caaagggttc gataccgttt tcaatcaact catcaaaata acgatgatag aaacgaacgg
    29821 cttcttcgtt gattgttcca gattcatcag gtatcaaacg gctccattgg atagatgttc
    29881 gaaatgaatt atgcccagtc tgtttcatca actgtacatc ctcttgatac ttcgtataaa
    29941 cttgcgaggt tttttcaggt cctacttgat caaagaactt ttgcggttct tttttgtacc
    30001 aataatcaaa aatgttctct ccttttccat cgcctgaaat ccttccttct gtttgtgggc
    30061 cacttgctgc agaaccccac caaaaatttg ctggaaattg atattttttc atcaaagcac
    30121 ctctttttct tttttagttc gttttagttg attccatgta tgatattttc catcacaatc
    30181 actttttcat tttcttcctc ttcttttttg attcgtttta acagctgttc tgctgctcgt
    30241 tccccgattt tatctgttgg tggcgtgatc gtgattttcg gaaggatact cagatcgttt
    30301 ttatctaaat aatcatagtg actgaacgca atatccttcc cgattctgac accttcttga
    30361 ttgaaacatt cgatggcacc aatcgacata ttgtaattgc tgacaaaaat agcagttgcc
    30421 cctttttcta ataatgcttt tgccccgtaa tagccgcttt ttctagaata gtctcctgta
    30481 aagacctcta attggatcga tggatcattt tggaccgctt ctttggctcc tgctaaacgt
    30541 tctcttgcta cgtagtctgt ttgagtggca gcaataaatc cgattttttt atgtccttgt
    30601 tcaccatgat gagcgatcac ttttgctaca ctttctcgat cattcactaa tattgtatca
    30661 atacctgctg gtccttctgg acaattcaat gcgataactg gaatactcat ttttgtgagc
    30721 atttcaaatc cagaccactc ttcttcggat aagaagacaa tcagcccatc gatcacatgc
    30781 ttcatcaggt attcaatttt ttgttcaatt ttttctggct gatcggcaaa cccgcttaat
    30841 aataagctat acccattttc ttcagctact ctttcgatcc ctgagacaac ttccgaccca
    30901 aaacggctgg aaatcccgtt tatcaaaagt ccaatagaga agcttcgatt attcttcaac
    30961 cctttagcaa tgatgttttc tttatagtcc aacgctgaaa tcgcttctgc tatttttttc
    31021 atgttctttt ccttcagttg ctgaccattc aagtaacggg aaactgttcc cactgaaaca
    31081 ccagccatct tagccacatc acggatcgat gccatacatt ctcactcctg ccagttacat
    31141 ttgatttctc tttcttgttg ttcgattatg atgaccgcat tctttgcata gtgttgcttg
    31201 tcatcaatat ccatataact aaagtcttca gtagagcaac ttcctctcac tattaattat
    31261 acttaaacaa gtatttgaac cggttcattt ttaatatagc atttttttaa aagattgcaa
    31321 cactttttta aaacgctttc tgaaagtggc ggattttttg cctttttaaa attttattct
    31381 aaaatttact ctatttgtgg gtgttttgtg cgtttttctc ttccatccgt ttgaagtttt
    31441 acattccaat ggtataattt ctttatccta tcaaatgggg gaagtatttt gaagaaatta
    31501 atcgtactat ttattttgtc tgcgggactt ttgacattga ctggctgtga tttgtttggc
    31561 ggctctgacc aatcctcgga cgaaacaacc gattcttctg tcttaaaagc ggaaaaagca
    31621 gataccacca acagtgtata tacgctaaaa gtacgtaaag atatggattt atccgcggga
    31681 cagttgacat ttacagataa aaaaatggaa tggacacgca cgtatcagac acaaggaact
    31741 gattcttctg aatcatctga agaaaaaaca gtgctgacag atatcaagat cactaccaaa
    31801 gatgacgaat atattatttc aggaaaagaa aataaaaaag aaacaacagt catatttaca
    31861 aaaatcggta attaccgttt gaaggatgag gatggcaaca tctacagtct atagtgccgt
    31921 ctgttggatc ttcttttgtt tctccccttc ttttctgcta tagtaaagaa gcaagatgat
    31981 tgtaaaggag acgaaatgga tgatcgatag tcctgattat tgtacatgta ataacaacaa
    32041 aaatatccat acagaattta atgaccaagg aagcttgagc atatgcgaca actgtcacaa
    32101 actagtcaaa gattcattta aaacatttga atcttatcct gttcaaaatt atgtaaaaag
    32161 tagaagaaga taaacaaatc caagcaaaac tcactatggc tgatttttat gaacttcaaa
    32221 ctcagcttct tcctttgatc aagcttcatc agtaaaacaa acagtggatc atttcagttt
    32281 acaaatcaaa gagtaaagat ttatttattt caaatatttg aatataaaat aaccaaacga
    32341 tagaaaggct tattctacat cacgtagtaa tttttctttc ccgtcgtttg gttttttatt
    32401 tttagcctaa ttcttgacct tttgcatgct ggttatccgt aaaatatttt ccagcaggct
    32461 ttccttccaa ttcagctaat ttttcttgga tctgacggat cacctttttt gcatcttctg
    32521 gattttctac aaaatcgaat ttatccccat ccacttgtat tttaggacat acatcaaaat
    32581 cttcatacca ttgattatag cgggtgttca acgtttcata ataggtatat aacgtctcat
    32641 cgctttctag ctgttcatag tcacgcccac gttttttgat acgttctagc atcgtgtcca
    32701 aagaaacttt gatatggacc atcaaatcag gccgtttttt tggagcaaca tcatctaact
    32761 cgtttagcat cgtatctaat aagctatcgt actgctgtac ttcgatatcc gttacccgac
    32821 ctaaatcagc gtttagatgg aacaacaggc tatcttcata gatggatcgg tctagcacat
    32881 tgtcatcgtt cactaaagca tttttcattg ctaaaaatcg cttgttcaaa aagaagattt
    32941 gcaataaaaa agcgtactgc tcaggatttg aataaaagag cggcaatact tcgttatctt
    33001 cgacattttc ataaaatggt ttagaatcaa tctcttttgc aagcatatcc gtcaaactgc
    33061 tcttacctgc accaatcgtt cctgaaatca aaatcactga catacaaccc ttcctcttct
    33121 ataagtactc gcctacgtaa gtttcaacag gcaatgtttc aaaatcatat tcacgtacag
    33181 cttggtctga ttttaaatac gtatggagac tttgtgtaat catcaagttc gtaggtaccg
    33241 tctcttcttg tacaatgatc atcggttttt tcaacatagt tgccacacct aattcgtaag
    33301 ccgttcctgg atcgattgtt tgatgctcaa agtcaaggat agcaacaaca aattctgcat
    33361 ttgtcagttc ttccatatct ttttcgtata cttcctgcgc ccatccagcg ctgaatagtt
    33421 cataattgct ttcttggtga tgacgtggag agtagaaact tgtgactgtt ttgttttcct
    33481 ccaacgcttt ttcgatacga cttactcgat cgatttgctc ctctgaaaaa aatggtcctg
    33541 ctaaatatat ttgactcatt attttgcctc cttcaataat aactgatttt taatagatac
    33601 tgctcgtaat tcattgacca gttccaatgg attgttggaa aagattactt gatccaacca
    33661 ttttttatcc ataaaatcct cttttgcaaa agtttctaat aattgttgca agccgctgta
    33721 ataaccgcca atattcaata ttccgattgg cttgtcgggc gtcagaccga ttgctatcca
    33781 agaaagaagt tgtgagaatt cctccaatgt acctagacct ccagggaaaa cgaggacagc
    33841 atctcccttt tcaaataata atacctttct ttcatcgatc gtttctgtag gaataaatgt
    33901 agtaacatct tttccaggca attcagtttc aaaaagccca ttcggataga tacctgtgac
    33961 ttttccgtta ttttccagta ctgttttact aatcactccc ataatcccgg agtatgaacc
    34021 accgtaaacc agttcgattt cttcttgtgc catgtatttt cctaacgttt gcgcaataaa
    34081 tttgtaactt tctttattac caaaacggga accgcaaaaa acagtaagtt tcataaaaaa
    34141 acactcccta actcaaaagt ctatcttcat tctacacgtg tttttttaaa ataaaagact
    34201 agattttgta gttttttagt ggtatatagg atagacgcca ctatatatag tatttagaaa
    34261 aaaatcattt ttcagccatt ttatagataa aacaaaaata aataacgtgt tttcatcgat
    34321 atgaaatgac ttacttatag aagatactgg gatttcgtcc tatatgccaa taacgtgtat
    34381 tattttaaac aacaaatact tgtttctttt attaacgaaa aaatcatttt gttctcgtgt
    34441 tatgtttcac tgataaatga tgtttctcta aataaaaaag ccgacgaact aaacgatcaa
    34501 aatttgatca tttttgttca tcagcttctc ctaaaaatct ttttgttaac gataaatagc
    34561 tggatttgta aaaatatcac ttgaatcgtc actgctggaa gaaaattcag tgataaccgc
    34621 acctgttgcg cctgcctgga actagtgttt gatattgggc tcgaacgtgt actgatctct
    34681 agcatgaaga ataatttctt tttttactgt ataataactt tctttccttt ttggaatagt
    34741 acaggaaagt ttttttgttt cctttccttc gacataaggt ataccagacc ctttcgacaa
    34801 cgaaaggttc tcatttttcc ttcatcgtca tttcaaggag ggtgccgatg ctctggacag
    34861 tttcgatcgc tcctaaacca aaatctgtat attcgattct ttctaactct gattctttca
    34921 taccattcct gtctcaaggt acatctattg tacttttttc ttgtaatcca ttctgcctct
    34981 cctaactaat tgtcaaatca tcccaagata tgtcttcttc ctctttttct tcttggatga
    35041 tttctgcatt cggatctact ggttttttca ataataaaat ggttattcct gtgacaaaag
    35101 aaccagctaa caatgcacca atgaatgccc atggtctggt catcgtagcg actgcaaaca
    35161 ttcccgatgc cggaatacgg ctatctgcgc cccaaaacat gcttaatccg ccacctactg
    35221 tcccaccgac tcccgttgct atgacattgc gtgccaagtc gttcaatacg atagggaaac
    35281 atccctctgt gatctgacag atccccatag gaaaagcggt cttcaatgta tcgatttctt
    35341 gacgattgag tatattttta cgaatgattt tccctaagaa ataagcgatc gtcaagccga
    35401 atggggttac catcgaagct aaaatcaata cagtgattgg ttcatagatt ccttctgaaa
    35461 atcgtgccac acccccttat ccagaataaa tggtaatcca gaaacagccg tgtatctgcg
    35521 gtaataagcc taaccaaacg aaaaatatga gaagctattt ttgtttggct gttcttatta
    35581 cttgatgcag ggcagctttt tcacaacctc tttttttatt tatgtttcat aatcaatatg
    35641 gtaaggatgc agctccgtat ggtagaccgc ctccgaatat tcaatcattt ccccaatttg
    35701 cgtagacgat ttccgtatcc gtttgatgcc gatctgtgcg tctgttttca gaattccttg
    35761 ttcttcttct gttaaaaata ctgccgagaa atcatcagaa aatgagtcaa tagtatatcc
    35821 ttttttcgtc agcaaacgat acaaagattc tttgccaaat tcttctctgc tgaattgcat
    35881 catttcttgt ggaagataat gaacaaaata aatataaggc tgattgttca aataatataa
    35941 gcgcttaaaa caaacagctt gctcaccgaa aaaggaaaac aaaggatctt ccttctttat
    36001 atggaccaat tgcaaatcca gcgtttcttt ccggacttca tatcgggagt tctccaagat
    36061 ttgcgtaaag cttgccgctt tggataatcg attgtaagga cgattgctta gaaccgtcgt
    36121 cccacgacca cttttctttg cgacatattc atctgatgcc agcagttcga ttgcctttcg
    36181 gacagtgatt ttgctgactt gaaacagttc ttctaattca ctttctgtcg gcaacatcga
    36241 accgactgga tattttcctg agaaaatatc ttctttaatt ttatctgcga cttccaagta
    36301 tagtacacgt gtccttttca taacgtcacc ctttgcttta ctattactaa aagaatagca
    36361 cataaaaaat aaaaaaagaa cacaaatcac atgtgagaag tgtgttcttt ttcattcagc
    36421 tgctttaatt acaagtttac gccatttgag gcaattactt ctttatacca atcaaaagat
    36481 tttttctttg atcgattcag tgtcccattt ccttcatcgt ctagatccac atagataaag
    36541 ccgtaacgtt tactcatttc tcctgttgat gcactgacaa gatcgataca tccccaaggc
    36601 gtatagccca tcaaatctac accgtctgct actgcattag ccatcgcttc gatatgacga
    36661 cgtaaataat cgatccgata atcatcaata atcgtgccat cctcttccat atgatcaatg
    36721 gctccaagac cgttttcaac aatgaataat ggtttctggt aacgattgta taattcattc
    36781 aatgcgattc tcagcccttc tggatcgatc tgccagcccc attcacttgc ctcaagaaac
    36841 ggatttttta ctcctcccag caagttccct tctgaagttg ctgcttgtgg atcagtctca
    36901 ttgattgccg tagacatata ataactaaat ccgatgtagt ccactggata ttcttttagc
    36961 aactggaagt ctttttccgt ttgctctaat ttttccggat cgactccata ttttttatgg
    37021 gtacgcgctg tgtatgcagg gtattctcca cgtacttgga catccgtgca gaaaaaattg
    37081 aattcttggt tttggatcat tgttgctgcc tgattgactg gattggcatc gatgctgtaa
    37141 gttgttgcat agataatcat gcagccgatt tggatatccg aacgcaattc gtggccgatt
    37201 tttacggctt tgctacttgc aacgaattga ttatgccatg cttggaaaat attttggtac
    37261 tctcctgctc cattgctttt tactaatcct tgacttaaag ctggaaagtg aaaggccgaa
    37321 ttgatttcat tgaaagtcat ccaatatttg actttactgc tgtatctctc taatactgtc
    37381 tgagcaaatc gttcatagaa gtcgatcagt tttcgatttt tccagccacc gtattctttt
    37441 gccaaatgaa gaggcatttc gtaatgagaa atcgtgatca caggttcaat atcatatttt
    37501 aagcactcat caatcagctg atcataaaat tctaatcccg cttcgttagg agtaccttcg
    37561 tctccatttg gaaaaattcg tgtccaggca atcgaaaaac gataacattt gaatcccatt
    37621 tccgcaaaca aggcaagatc ttctttgaaa cgatcgtaat gatcaattcc tcgatgattc
    37681 gggtaaatat atttttcttg atcaatcgtc cagtcaaatt cttcactgcc gataatcgca
    37741 aagcgctgct tacctccagg catagcatct gcaacggata gcccttttcc gtctcgatca
    37801 taagcccctt ctaattgatt ggcagcagtt gctccacccc ataaaaattt ttctgaaaaa
    37861 cgtgattgca tcctgatttc ctccttattt tacaattgcc agaacatctt cgtttgcaat
    37921 aatctctttt tggtctaatt caagtatatc ggcaaatgca tcagtattcg taatgacaac
    37981 tggagtgatt acgctatagc cagctgctct aattgcttcg atgtcaaaac gaatcaataa
    38041 atctcctgct ttgacgagat ccccttgttt gactaagatc tcaaagccat tcccatttaa
    38101 ttctactgta tccatcccga tatgaatcaa aatctccgct ccgtcagtcg ttgtgatccc
    38161 tacagcatgt ccagttggaa aaatagtcgt gatctctccg tctgcaggtg caactagttc
    38221 tcctacttct ggatcaatag cgataccttt tcccatcacg ccagaagaaa aaacttggtc
    38281 tggtacttca ctcaatggca gtactttacc cgttaaagga ctttttaagg tatgccgtgc
    38341 tgaagtaatc ggttctcctg cattcgcatg tttgttttct aattgattgt cttgtgtttg
    38401 ttcgtcaaat cctaaaatca atgtacctac gaatgcaaga acaaaagcaa ttcctgtagc
    38461 tattactgct actgtcacat ttgactctac accatccacc gtattaatga aagtcggaat
    38521 cgtcaataag ctgaccaaac tgctggagaa agcttttact ccggaaaatc cgataatagc
    38581 tccaccaata cctccagaaa tacaagcggc aatgaatggt ttcttcaaag gtaatgtcac
    38641 accatacaca gttggttcag tgattccaaa taacgaagtc actgtagaag aaatccctaa
    38701 tgctcgaagt ttcgtatctt ttgttcgcaa tgctacagct aaagcagccc caccttgtgc
    38761 caagatggct ggaagaagca ttggcatcaa tacatcaaac ccatattgtt cgatattcaa
    38821 aaacatgatc ggtacgaatc cccaatgcat gccgaacatc acgaatactt gccataaact
    38881 tcccatgatt agacctgcta cgattggact aaatccataa atcgagttga agagaccgcc
    38941 taaagcatta ccggcaaccg tcccgattgg tcctaaagca agaaatgtta atggaaccat
    39001 cactactaaa acgacaagcg gaactaaaat catttgcaga aattgcggaa tcactttttt
    39061 tacgaatggc tctaatttac tttgaaccca tacggctaga atgattggta tgacagaaga
    39121 agtataacca gatggtgaaa gaatcactgg gatcccaaag aaactgatag cttctcccgc
    39181 accagcaact gctgtgatag ctggatacac aagtgccata gcaattgcta ctgctaaaaa
    39241 ctggttggct ttgaactttt tagctgctgt aaaagcaagc atgacaggta agaaggtaaa
    39301 gacaccatct gctgcagcaa acaatatttg ataagtaccg ctatcagctg tcagccagcc
    39361 catcaccgtt gccaatgata agaatccttt caataccccg gcggctgcca tcgcccctag
    39421 aaaaggtgta aatatactag agataatatc gatcagacga ttcaaaatat ttttggactc
    39481 ttcttttcca tcctccgaaa ctgcaagacc ttcctcatcc acgatcgatt gataaacatc
    39541 tgctacatta ctgccaatca ctacttgata ctgaccgcca ctttgtacga cttggatcac
    39601 gtccgggtct tcttttaact tttgcgtatc tgctaaagat tcatctttta atctgaatcg
    39661 caaccgcgta gcacaatgta ctaaactttt gacattcttt tgtcctccta ccgcttccca
    39721 tacacgacga ccaactgctt gattatccat tgttttttcc ttctttcttt tttatttaaa
    39781 aaataaaaac ccagattgaa aaaaacagtc acaaacattc gtgaacctat tttttcaatc
    39841 caggttttgc ctgccataca gtaacaatcc ttatgattgg atgctttttt tctcacttac
    39901 tactctttga atatgaatcg ttaaataaat ctgctctgcg atccccattt cctgtttttg
    39961 cgtttgtcga agatactcat tgatccgttc gacacaatga aacgcttttg gatactttcc
    40021 ttgaactaat gcgtagagaa attcatcttg ctcgtcagat ccttcccctt tgagataacg
    40081 ctgggcaaaa aactgcagat gagtaatgat acgctgataa ttcaaagatt cttcatcgaa
    40141 cacgatgccg aagaatttac tgatgatact cagtatatct cttaccattg tggtagcggt
    40201 catcgtcact tgcatgtctt cgtttccttg ttggctattc acaaaatgaa aggcaataaa
    40261 accggcttca tcctcagaaa gttccacacc cgtcttttgt ttcacgaaca aaagagcctc
    40321 tttcgctgct tgatattctt tcggataaaa tttttttgtt tcaaataata atggattagg
    40381 cagttggata ttttcttttg ctcgtgtgat cgcataatga agatgatcgg tcaatgtcag
    40441 ataaatatta ggcgaaaatt caacttttag tttatcttca gccagttcga ctacttgttt
    40501 gacccattcg atctcctctg ccggaatatc agccatcagc tgctggaatt ggtttttagc
    40561 aaaagaatct cttaaaacaa attctttttg gaccgcttct ggcgaaatct cgtccccaat
    40621 ctttttttga aaagctagtc cttttcccat acaaacgact tcttccccga atccattttt
    40681 cgaaatgacg acattgttat tcaaaatttt atcaattttc attttgcttc gccctctcaa
    40741 gaaaaataaa aaaacctaga attcgcctac tgtttcttag ctgaattctg gttttgcctg
    40801 ccgtacagta acaatccatt tcttattcaa aatcatcata ccatcgaatt tttatttatg
    40861 caagcctttt catataaaaa ataaaaaaac tggaacacaa gtctatctag aaaacaacca
    40921 aatcagagat gcacctcttt gcgtttctct ttttttcagc aaattggttt ttccttatcg
    40981 attcatatcc tttggctgtt tttaatctgt aacgacttat gtcccagtat cctaaaatgt
    41041 ttttgtctct agattgaaag cagatctcat atcatctgtc gcatctttta tcgatgtaac
    41101 actttccagt tctccttgta caactagtcg cgtgacggcg tccatgtcgc cacaagtaat
    41161 tagtgtgaca atttcttttc cctcgacttc attcagtaat tgggtatctg taggagcaac
    41221 tttttcttta aaaaatgttt tgtatgtata cacattttct aaatctgtca aatagatctt
    41281 ggcacccatc tctaaatttt ctagcggtgt aaacagcaat tcagggttcg ttgcacgatg
    41341 gcttgctaag gcataatttc cttttcccat ttcttgatct ggagataatg ttcctgctcc
    41401 gtaaagtagt gacgtattat ccaagccttt gaaaaccggt aaattgattg aaactgaagg
    41461 gactgcgata ctagcaatca ctggtagatc tgttcctctc atttgcgacc tcatgacccc
    41521 ttctgtcgtc attggttcta ccgcatcaaa gtcaaaactg acatctttat cgttattctt
    41581 tttcagatct tcttttgtta cattcgctat agcatattta tctcctgtct ctctgacaaa
    41641 ataatccttg atctgttcat tgaaaatcaa agctagtcct actaataaca agaggaacaa
    41701 gaggatattg actagccatc tttttgcttt tccttttttt ttcattttct ctccctccag
    41761 actcatttta ctgtttttct tttgctcttt ccaacagccg aatgacacat ttctacaact
    41821 ctgtgtattt ttttcgtaca cctgcgcttt tttctacagg atatttcaaa ttatttttga
    41881 tcaatttttc ttcaatgatc tcatctagat ccaggtgaag gttgtctgcc atcatgtatg
    41941 aatagatcaa tacatctgcc agttcttctt tgattcgttc taattgtgtt tcttttactt
    42001 cttccggcgt tttccattga aaaagttcta acaattcaga tgcttccaaa gaaatagaga
    42061 tagccaaatc tttttcgtta tgaaattgtc gccaattacg ttcatctcta aactggttga
    42121 ctttttccat actattcatt tgcttgcctt cttccaatca cttcttactt gattaatcat
    42181 accatttttt tacataaaaa atagatgatg cttttaaaaa aactcctgtt ttcttttatt
    42241 taaaataaat gtcatagaaa atcaataatt taggtagcca ccatgtgtat gcagtagcgt
    42301 gtcttcttcc cttgaggctt tttgtttttc gatacgttct tcttcccaaa cagacaacgc
    42361 tactagaatc tctcttaatg attttccttc ttcggtgagc gagtactcta ctttaggcgg
    42421 gacttggtta taggcttttc gatggatact ttcatcttgt tctaactctc ttaactgctg
    42481 ggtcagcatt ttttttgtga tcgtagggat ttgcttgctt ctccacgcct aaaataagcg
    42541 atattgctct tccctttatc tggtgtaagc aaagcagtta gcattttgac gatcgtgggt
    42601 tccgcttttc acttcttatc tacttagtat catgaagtat actttcgtac taatactaag
    42661 tagcggaagt gtcaactact ttgtttaact acatagcaaa agaaacagaa gggtgacaac
    42721 ggtcatcacg cttctgtttc ttttgaatat gctgtgttgt tgtctctatc tccgtgtaga
    42781 ctttcctcaa acttatcctt tgacagtaag tcagtaggct gatttgctct ttctagatgc
    42841 cagatacatt tctacaaaaa aatagtgatt ctgttttgaa aattcgattt ttagttctgc
    42901 attttcgtaa acatccaaca tttttttcgc actgtataga cctaacccgc catttttttt
    42961 agaggttttt cctcttttga ccatctccga tagcgtcaaa ttcgttggat ttgccttgtt
    43021 cgctatacta aaacgtgatc cctctttttc ttccagcaga ttgatcgtaa tcggactctg
    43081 atcggctaca cgatgatcga atgcgttaga aataaaaata gataaacagc gaataaaaac
    43141 aaccagatca atcgggattg ttttcacttc gacaggaata actacttgga tcgggatttt
    43201 ctccgattct gctttttcta gaaattttaa aaagacaccc ctgacagctg gggatacaat
    43261 atttgttagc tgttgtattt gcggtttttc aaaaatagct gcagaatgat cttctaatat
    43321 ttctaaaaat tccaacgttt cttttttatt ttcctgaaga gtaattttta atgaaaacag
    43381 tagactttta taatcatgtt tgaattcctc tagatgatgg taatattctt catttatttg
    43441 atacagttga agcgattcct gatgttgtct ttcttttact tggaaactga tcaacataat
    43501 ggaaaaaaac aaagaaaaaa aactgatgac tatcgacaaa agagatagaa gcagataatt
    43561 agatgagcgg aagtgaacac ttcccaatac caagtaaatc acgccccagc ttaataaaat
    43621 acaaatacct ccaaaatagt gttcagttgg ataagtaaaa agcaagttga gaaatctcgt
    43681 ctgtttgtct atacttttta ctaatagata gaaaataaat gcgataagta gtgcaaaaaa
    43741 acttgctgtt ccaagatgtc tctttcctaa ataatccatc agatcaaaaa ccagaaaaaa
    43801 gctgagtcca gtgatagcaa aaaaaagatt actgatcaaa cagctgataa acaggttttt
    43861 cgtagtaaaa taaaaaagaa acatcaataa taagccaatt aggtaaggaa agctcacttt
    43921 agggatagga agtaaaagtg tcaccaccat caaagctata caaccaaagc tgttttttac
    43981 tagcagttgt ttcgaaaaga ataaatagat acctaaatct aaaatcaaag taaaggtcaa
    44041 ataaagtgtc actgttgcga ccatcttatt cctccattgt tttgtttcaa aatgtacaca
    44101 tattttttta ataggagcct gcatatgaaa aagtatttta tcatagaaga taaccagttt
    44161 cacagcgaat ggatcatcag agaattggtc aatcatcata tcgagaatat cgtgcagctc
    44221 acttctatct ctcaaatcaa tcagttgatc cagttgggca ctattgataa tcacgatgtt
    44281 ttctttatcg atatcgactt gaagcaatca ctcacaggta ttgatgtcgc cgaacaaata
    44341 cgtaaaaaaa atgcacatag taatattgtt tttttcaccg cttatacgaa ttttgcagta
    44401 gagatcatca atcggaaaat ccttccgatt ggatatatca gaaaagacag gaatttatct
    44461 gaacaaattc gtcaagtgct agaacagatc aattggaaag aacagcagat cgtaaatgtc
    44521 gataaaatct ttattgatca aaaaggtgga agtc
//
LOCUS       DAXLPI010000011        43668 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXLPI010000011 DAXLPI010000000
VERSION     DAXLPI010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.011"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(307..2688)
                     /locus_tag="ACF3T5_000506"
     CDS             complement(307..2688)
                     /locus_tag="ACF3T5_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFD3631141.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2678..3325)
                     /gene="recU"
                     /locus_tag="ACF3T5_000507"
     CDS             complement(2678..3325)
                     /gene="recU"
                     /locus_tag="ACF3T5_000507"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFD3631142.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3389..3928
                     /locus_tag="ACF3T5_000508"
     CDS             3389..3928
                     /locus_tag="ACF3T5_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFD3631143.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4018..4437
                     /gene="gpsB"
                     /locus_tag="ACF3T5_000509"
     CDS             4018..4437
                     /gene="gpsB"
                     /locus_tag="ACF3T5_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFD3631144.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4459..4826
                     /gene="rnpB"
                     /locus_tag="ACF3T5_000510"
     ncRNA           4459..4826
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACF3T5_000510"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5035..6201
                     /locus_tag="ACF3T5_000511"
     CDS             5035..6201
                     /locus_tag="ACF3T5_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFD3631145.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6247..7743
                     /locus_tag="ACF3T5_000512"
     CDS             6247..7743
                     /locus_tag="ACF3T5_000512"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFD3631146.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYGIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     LLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7824..8507
                     /locus_tag="ACF3T5_000513"
     CDS             7824..8507
                     /locus_tag="ACF3T5_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFD3631147.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9152..10342
                     /locus_tag="ACF3T5_000514"
     CDS             9152..10342
                     /locus_tag="ACF3T5_000514"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFD3631148.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10444..11397
                     /locus_tag="ACF3T5_000515"
     CDS             10444..11397
                     /locus_tag="ACF3T5_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFD3631149.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11466..12815
                     /locus_tag="ACF3T5_000516"
     CDS             11466..12815
                     /locus_tag="ACF3T5_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD3631150.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12998..13321)
                     /locus_tag="ACF3T5_000517"
     CDS             complement(12998..13321)
                     /locus_tag="ACF3T5_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631151.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13450..14613
                     /locus_tag="ACF3T5_000518"
     CDS             13450..14613
                     /locus_tag="ACF3T5_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFD3631152.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14683..16032
                     /locus_tag="ACF3T5_000519"
     CDS             14683..16032
                     /locus_tag="ACF3T5_000519"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFD3631153.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16077..16508)
                     /locus_tag="ACF3T5_000520"
     CDS             complement(16077..16508)
                     /locus_tag="ACF3T5_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3631154.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16896..19538
                     /gene="alaS"
                     /locus_tag="ACF3T5_000521"
     CDS             16896..19538
                     /gene="alaS"
                     /locus_tag="ACF3T5_000521"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFD3631155.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19738..20334
                     /locus_tag="ACF3T5_000522"
     CDS             19738..20334
                     /locus_tag="ACF3T5_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFD3631156.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20456..21604
                     /locus_tag="ACF3T5_000523"
     CDS             20456..21604
                     /locus_tag="ACF3T5_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFD3631157.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21642..22055)
                     /locus_tag="ACF3T5_000524"
     CDS             complement(21642..22055)
                     /locus_tag="ACF3T5_000524"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFD3631158.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22134..22565
                     /locus_tag="ACF3T5_000525"
     CDS             22134..22565
                     /locus_tag="ACF3T5_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFD3631159.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22664..22897)
                     /locus_tag="ACF3T5_000526"
     CDS             complement(22664..22897)
                     /locus_tag="ACF3T5_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD3631160.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23080..24750
                     /locus_tag="ACF3T5_000527"
     CDS             23080..24750
                     /locus_tag="ACF3T5_000527"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFD3631161.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24897..25283
                     /locus_tag="ACF3T5_000528"
     CDS             24897..25283
                     /locus_tag="ACF3T5_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631162.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25270..25968
                     /locus_tag="ACF3T5_000529"
     CDS             25270..25968
                     /locus_tag="ACF3T5_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFD3631163.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25973..26437
                     /gene="lspA"
                     /locus_tag="ACF3T5_000530"
     CDS             25973..26437
                     /gene="lspA"
                     /locus_tag="ACF3T5_000530"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFD3631164.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26434..27345
                     /locus_tag="ACF3T5_000531"
     CDS             26434..27345
                     /locus_tag="ACF3T5_000531"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFD3631165.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27580..28119
                     /gene="pyrR"
                     /locus_tag="ACF3T5_000532"
     CDS             27580..28119
                     /gene="pyrR"
                     /locus_tag="ACF3T5_000532"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFD3631166.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28133..29416
                     /locus_tag="ACF3T5_000533"
     CDS             28133..29416
                     /locus_tag="ACF3T5_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFD3631167.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29429..30355
                     /locus_tag="ACF3T5_000534"
     CDS             29429..30355
                     /locus_tag="ACF3T5_000534"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFD3631168.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30374..31654
                     /locus_tag="ACF3T5_000535"
     CDS             30374..31654
                     /locus_tag="ACF3T5_000535"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFD3631169.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31659..32735
                     /locus_tag="ACF3T5_000536"
     CDS             31659..32735
                     /locus_tag="ACF3T5_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFD3631170.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32740..35925
                     /gene="carB"
                     /locus_tag="ACF3T5_000537"
     CDS             32740..35925
                     /gene="carB"
                     /locus_tag="ACF3T5_000537"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFD3631171.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35979..36755
                     /locus_tag="ACF3T5_000538"
     CDS             35979..36755
                     /locus_tag="ACF3T5_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFD3631172.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36752..37684
                     /locus_tag="ACF3T5_000539"
     CDS             36752..37684
                     /locus_tag="ACF3T5_000539"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFD3631173.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37681..38397
                     /gene="pyrF"
                     /locus_tag="ACF3T5_000540"
     CDS             37681..38397
                     /gene="pyrF"
                     /locus_tag="ACF3T5_000540"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFD3631174.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38404..39033
                     /gene="pyrE"
                     /locus_tag="ACF3T5_000541"
     CDS             38404..39033
                     /gene="pyrE"
                     /locus_tag="ACF3T5_000541"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFD3631175.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39021..39335
                     /locus_tag="ACF3T5_000542"
     CDS             39021..39335
                     /locus_tag="ACF3T5_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631176.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39373..40014
                     /locus_tag="ACF3T5_000543"
     CDS             39373..40014
                     /locus_tag="ACF3T5_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFD3631177.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40057..40749
                     /locus_tag="ACF3T5_000544"
     CDS             40057..40749
                     /locus_tag="ACF3T5_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFD3631178.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40746..41648
                     /locus_tag="ACF3T5_000545"
     CDS             40746..41648
                     /locus_tag="ACF3T5_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD3631179.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41806..43512)
                     /gene="efbA"
                     /locus_tag="ACF3T5_000546"
     CDS             complement(41806..43512)
                     /gene="efbA"
                     /locus_tag="ACF3T5_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFD3631180.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13990 a         7693 c         9487 g        12498 t
ORIGIN      
        1 tcaaaaccat atattgtatt ttttatttga taataacaat gggattatta agaagtggca
       61 ctaaataaaa gactagtgtg tatttagccg atttaattat aaacaagtaa atggataaag
      121 tatatgtaat gataaaagaa aaatggattt tctaattttt aagctaaatg ttagatcaca
      181 catgtatatg ctggatagtc ttataaaaaa tcatgtaata cagcttggta catgattttt
      241 atttgtagct gttttttaca tagacacaaa aaagaaacca cgcttgtaga cgtggtttct
      301 tttttactat ctgtttccag aagctgaggt gctgctggat ggtgtatggt catttgcagc
      361 atccgctccg ctagttgctg gctcactgga agaagcagga ggagcagatg aagacggtgt
      421 cgagctttct tcggatgaag aagaaggaac cgttgttgaa gacgctggct cgcttgtttc
      481 tgctgtcgtg ctttcttttg aggactcagc agttgattcg ctttgactag tagagctaga
      541 gctttgagtc gttgtctctg ttgttgagct tccaggagtc tggacatagc tggaagatgg
      601 aatagttgtt gatggtgtaa ttgcattcga tctagctgta tattgatctt tgtagtacag
      661 ttcaccgccc acacgaatta atccgcttgg catctcccag tctgtatttg tcacgttagc
      721 tgaaacatac tgcataagct cgcgataaac atcagaggct acgtgtgacg attctgaagt
      781 gacaggtgtc atttttttat tgtaacctgt ccacacggaa atcgaataat ttggcgtata
      841 gcccgcaaat aggatatctg gatacacccc agaagaaata ccaagttttg catactcgtc
      901 atctgtgtaa ttggatgttc cggtcttacc ggcttgatat aacccagcga tttgcgcatt
      961 cgtaccagtt ccttcagtga tcgtatcttt taaaatgtct gtgatcatat aagctgtttc
     1021 tggagacatg gctgtttttc catctggctc ataagtttct tctgttccgt cttggaagac
     1081 gattttatta acatattgag gtttgtaata agtccctcca ttggcgaaag ccgcataggc
     1141 tgctgccatt tttaaggaag aagctccgta ttttgtccca tcttgttctt ccgtattact
     1201 agagatcgca ttcgattgat ggatcgtact gtattcgatc ccaagatttt ttaagaaaga
     1261 agccacttta tctgaaccga cttcattgaa taatttgact gctggaacat tacgggataa
     1321 gtaaagtgct tggcgtaaag taatggtgcc catatattga ttatcccagt ttcctacagg
     1381 agtcgaagtc ccttcatagt tatacggtgc gtcagtaata gtttttccag tagaatattt
     1441 caaatattca aaagctggtc cgtaatctgt gacaggtttc atcgtagaac caaaatcacg
     1501 ggaagtattc actgctaagt tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc
     1561 aatctgggct tttactttcc cggtattggt gtcgataagg gtggaggcaa cttgcatctc
     1621 gtcgtctggg taagagacat actgatccgt gttgacgata tcatatagtt tcttttgggc
     1681 gtccagatcc aaattcgtat aaatctctaa accgtctgtg tagacattct tatctgtttt
     1741 ttcttgtact tcattgatga cttctttgac ataattatcg acgatcttag tattgtcatc
     1801 ggattgagtt agttcttgca gaccatccgt taccggtaca ttaacagctt gatcatattc
     1861 tgtttgagag attttttcgt tttgaagcat cgtatacaaa acagtatctc gtcttttctt
     1921 cgcctgatct ggataaacat aaggatcata ggcggaaggg gcttgaggca tcccagcaag
     1981 caatgctgtt tgcggtaaag aaagttctga taatttctta ccaaagtaca tctcagaagc
     2041 tgtctccatc ccgtacaagc cgtttgacat atacacttta tttacgtagt aggtcaagat
     2101 ctcttgtttc gatttttttt gttccaatct tacagccatc cacgcttctt gggccttgcg
     2161 tttcagagtc tgatcctcgg cactggtgga gaagaaggaa agtttgatca attgctgtgt
     2221 caaagtactt ccgccttgca gtccgccaga ggtaaagtta gagagggcag aaccgatgat
     2281 ccgaataggg tcaaccccga tgtgtttata aaaacggcga tcttctaccg acacgatcgc
     2341 atcttctaat gttttcggta attcattcgc agaaattttt tcacgctttt ctgcgcctaa
     2401 atcttcgaat agttcgccat cttgtgtata tagtttggaa gatacggtag cgtctaattt
     2461 tttatccgtc aattcgggcg catcttttgc gtaataccag aaaagtccta cacctgctaa
     2521 aaagaggata cagaaaaatg agagtattcc taaaaggatc ttgataaata atcctttttt
     2581 atgtgttcca gaggactttc cagaaccttt gccagaattc ttcttcacac tctttttagg
     2641 agtaggctgt ttctgtcgtc tagagcttct agtttgttca tttgccattc ttgctttctc
     2701 cttcaataga taatgttgat aaatagaagt caaccgcgtc caaataagga atccgcggag
     2761 aaataccagt tttgatctct attcctagtt cttcgatcaa agataaaggt agtgatttgc
     2821 gtcctttttc cttttgggcg tcccagtaag caatcaaatg aacactattc aaataaaaac
     2881 agcgcttcaa agacgaaaac cataacaaga caaagcagac cccattttgt tcgaggcatg
     2941 ctttcatatg ttcgatttga tgggcatgga agtttttaaa gggaaaagat gttttgttct
     3001 tggtttcttt tgcttcaaag tccaagtaaa agcctcgata gacgccatta tagtctgtag
     3061 ttgaggcttg ggtaaagtag gcttccttga tcacagcagc acttctctta ggatagtcta
     3121 ctttcacgat ttggataggg gtaggctttt tatggataac cgccaacccg cgggaaagat
     3181 aatatttatt actttcattg attgcttctt caaagcgcat cccccgattg ccgaattcag
     3241 tgatctgatt tcttttttct tgaatttttt cgacgggaga ggcattctta taataaggtt
     3301 ggccatttgg atagcgcaat accatcttta atcacactcc taggccacat tatagcaaaa
     3361 aagcggatag aaagaaaagg aatctgagat ggataagtca aaagtaatct atgtgtctgg
     3421 gtatcgaagt ttcgaactcg gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa
     3481 aaatgtttta aaaaaagaac tccatcagtt agcagaagaa gggctggaat gggtacttgt
     3541 cagtgggaat ttgggcgtcg aactatgggc tgtgcaaata gtagcggaat tgaaagaaga
     3601 atatccggaa ctatctcttg gtattttgta tcctttttct gattttggta ataactggaa
     3661 tgaagggaac caagaaaaaa aacaattagc agaacaatta gcagattatg tcgaagctgt
     3721 tagccatcaa ccctaccaat cacctagtca gctaaagaat cacactcgtt ttatacttga
     3781 gcatacagtg ggctgtctgc tgatctatga tgaagaattc cctggaaaaa cgaagtattt
     3841 ttttgaagat gcacagaatt accaagcaga tcatccctac gaaattcggc tgatcagtat
     3901 ggacgatctt caaaatttta gtgaataatc gatattgtgt ttgatttatc aacttttttc
     3961 ctatacaatg aaaaaggtag aaatgttatt acgtaaatga aaactagagg tgtaaatatg
     4021 gcgaatttgg tttatagtcc taaagacatt ttacaacaag aattcaaaac aaaaatgcgc
     4081 ggctatgatc ccgttgaagt tgatgaattc ttagacaata tcatcaaaga ttatgaaact
     4141 tacagcaaag aactccttgc tttacaagaa gaaaacgatc gattgagtgc aaaagtagct
     4201 caattatcta aaactcaagg agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag
     4261 agcgcagcag tgaccaattt tgatatctta aaacgcttgt caaatcttga acgtgaagta
     4321 tttggtaaaa agcttgatca acaagcaagt gcagtcaaac cagcacaacc taatccaaat
     4381 aactacacaa atgcagacac aagcttagat gataatgaaa aaacacgtca attttaattt
     4441 ttgattaatg aatttgcggt tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa
     4501 gtccatgctc gcacaggctg tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta
     4561 aggtactctt tggagtaacg gcaggaaaac aagctaaggc ttcggctatg cttaagtatc
     4621 cttgaaagtg ccacagtgac gaagcggctg gggaaaccca tccggtggaa cgcggtaaac
     4681 ccctcgagcg agcaacccaa acaatggtag gggcgctttt tgtctggaag aagaacagga
     4741 caaaaaggca gtataactgc agatagatgg ttaccgcttt gttttttctc cctggaaaag
     4801 caaagtacag aacatggctt atagaaaacc gcaaacatca gggaattagg tcgtgacaaa
     4861 agtaatgcgt tataagaaac aagaaaaaca tttgaaagct atttttcaat acttcagatg
     4921 aatcgttctt attacaaaac aaacttttgt atcaccggtt attcggaatt tgagtaaaag
     4981 tgtggggtag gactgatgtc ccacctcctt tttttataaa taaggagatt gtgtatggaa
     5041 aaaaacagga agttcaacct cgtcgcaact gcggcaagcg ggctagaagc actggttggt
     5101 aaagaattaa gagatcttgg aatcgaatgc caagttgaga acggacgtgc acgttttgaa
     5161 ggaacaatgg aaacgatcgc tacagctaat ttatggttac gtacagcaga tcgagtaaaa
     5221 atcgtggttg gggaattcga tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca
     5281 ttgccttggg aagattttct tccactagat gcagcttttc ctgtagctgg aaaatcgatc
     5341 aagtcgaaac tatatagcac acctgattgc caagcgatca caaaaaaagc aatcgttgaa
     5401 cgtctccgta cctattatca ccgtccagct tctgtaccac tgacggaaac tggcgcacat
     5461 tttcagttgg aagtagcttt gctgaaagac catgtgatgg taactctgga tacgacagga
     5521 ccaagtttat tcaaacgtgg ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac
     5581 atggcagcag cacttattat gttgacaaat tggcgaaaag accgtccatt ttatgatcct
     5641 gtatgtggtt caggtacgct ttgtattgaa gcagccttaa taggccataa tatcgcacca
     5701 ggattcaatc gtgagtttgc ttgcgaatct tgggattggt tcagtcaaga aatcatggaa
     5761 aatgttcgat cagcagccga agaaaaagca gattatgata ttgaattaga tattactggt
     5821 tcagatatca atggacggat gattgaaatt gccaaagcca atgcagaaga aatcggatta
     5881 ggctcttcga ttacctttaa gcaacaagca gtcaaagact tcaaaacgga taaagaatac
     5941 ggggtcatcg tggctaatcc gccttacgga gaacgtctag gagaagaaga aactgttcgg
     6001 aaattatata aagaaatggg cgatgtattc cgtccattaa aaacatggag taaatatatc
     6061 ttgactagcg acttagcgtt tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa
     6121 ttgtacaatg gtgcattgcg tacggatcta ttccagtatt ggggaactcg tccgcctaga
     6181 aaaaaagaga aagacgaata atcttactaa ttgattcatt caattggatg tttggaggaa
     6241 tagggaatga aagaaaaaga gtttttacaa gaagtaaaag aaatcaatct tctacaacaa
     6301 gctttaggaa tcctcgattg ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac
     6361 cgaagtgaag tagacagcta tctttacggc atttattttt caaaaaaaat cggtccaaaa
     6421 atcaaagaag ccattcgcta tttttctgaa catccggaag aattgtcgga agttggaact
     6481 gccgtttttg ccaaagtaaa agaagagtat gatttggaac atcgcgtacc ggaagatctt
     6541 atgagaaaat atacagctgc cgtttcttca gcacacggac aatggcaaaa agctagagaa
     6601 acacaacaat tttctgattt tcaacacgca ttgaatcaaa atattgaatt aacaaaacag
     6661 ttgatacctt actggagaaa agatgaaaag acgaattatg atgttttgct gaatcaatac
     6721 gaacccggga tgactgtcga aatccttgat aaggtatttg atcagcttaa agaaggaatt
     6781 ctttcgatcc gccgtacgct ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat
     6841 cggagaatga cgaaaaaaca acagaagaga tttgtagtga aagtaatcga acaacttggt
     6901 tatgatttta gtcgtggtcg tttagatgac actgtccacc catttatgac tggaattaat
     6961 ccgaatgatg cacgaatcac gacacgatgg aacgaaacag atttcaaaat ggctgttttt
     7021 ggagtgatcc acgaggctgg tcatggtatg tatgaacaag acatcgatga taaatatgcg
     7081 tatacaccgg tttatgaagg tacatcaatg ggtatccatg aatcacaatc gttgtttaac
     7141 gagatcatta ttggaagtag tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa
     7201 tgtgcagaag gaacattcga tgatattcct tttgacaaat tttatcgttt attgaaccat
     7261 acaaaatcaa gtttggttcg tatcgaggca gacagtttga cttaccctct ccatatcatc
     7321 attcgttatg aaatcgaaaa aatgattttc aatgaagggg tagatgttgc tgaactacct
     7381 gaaatatgga atcaaaaata tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa
     7441 ggcatcctac aagacgttca ttggtcaggt gcaagctttg gttattttcc ctcttatgca
     7501 ttagggtaca tgtatgcggc acaattactt cattctttgc agcaagaaat agatgtggaa
     7561 aatgttctag cgtcagacga ttattctcca atcaaaaact ggatgtcaga gcatatccat
     7621 caatatggtg catctagaaa accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt
     7681 aatccgcaat acctgatcga ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa
     7741 taaaatacaa tttacaaaga tagctgaaat tcatctaatc tatagatgtt ttcagctatt
     7801 tacttaagaa agaagtggaa cgaatggata aattacaagt gatcgctgat tatagtatgg
     7861 accaattagc gcatgatcaa acaggacatg ggagcgatca taccaaaaga gttgtcaagt
     7921 tagctgaaaa aattttggag acggaaccgc aggcagaccg ctttgtgacg cttgcagcag
     7981 cttacctaca tgatacgatt gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac
     8041 agcttcgtgt attccttcgc tctttgccaa tagtagagga acagatctcg atgatttttt
     8101 ccattatcga aaatatgtcc ttttctaaaa atctctctga agcggccgaa ctatctctgg
     8161 aaggtaagat cgttcaagac gctgatcgta tcgaagcatt aggagcaatc ggcattttgc
     8221 gtactgctta tttcggcggt ggacatgggc acccgatttt tgatcctgaa ctgtatccgc
     8281 aaacattcaa ggacaaagaa aattaccgaa aaggcacgac agtgatcaat catttttatg
     8341 aaaaactgtt cttgctgtct gataaaatga atactgatta cggatatgaa gaagccaaac
     8401 gaagagaaac cttcatgcgt gaattcttag aagaattttt tctggaatgg tctattgacg
     8461 atgaaaattt tactccagac aattggatca aaaataacaa aaaatgatgg aggttgtgac
     8521 agaagtggtc agctccaaga tatttcggat cattttccgt aaaagttact tctggaacac
     8581 tgtttaccaa gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa
     8641 aaatagcttc tcgtgtttga tgaatattgt aacttgtgat tcggaattgg caggagaaca
     8701 ccgtttattc ggaagaatag gactgtgaca agacttttgt cacagtcctt ttttcgaatt
     8761 caacaagtaa aacttttaag aaatattcag agagtaaaat taaaaattgc tgataaacga
     8821 agatggaaat tttatgtatt tttattggtc tatgcaataa ataaattaaa tattcagaat
     8881 acaattgatt ttaacgaaag ggcttctatc aatggttgtg ttgtatttat ctgcccaggt
     8941 agagtaaaat agatcattat ctaaaaggta attgttcata ctatataagt catttgaaag
     9001 taaaaagata ctgtaaataa aaaatgtggt taaataattc tcataatcag tgtaagcgtt
     9061 atcaaaaata tagcatactt gctttgtaga tgaatgacac gatatatgtt tggtatgttc
     9121 tgcaagacag atgaatatgg aggtggagac aatgtcttcc aaagaactaa caatgtattt
     9181 agagaaagaa ttggatcaac tgaaagaaaa ggggttatac aatacgatcg acatactaga
     9241 aagtgaaaat ggtgcttgta tcatcgtaga cggaaaaaag atgatcaatc tagcatccaa
     9301 caattatctt ggatttgcca atcgcgaaga attgaaaaaa gcatgtattg aagcaaccga
     9361 aacttatggt gtaggagcag gagcagtccg aacgatcaat ggttcgctga aaatccatca
     9421 gcaattagaa gaaaaaatcg cagaattcaa agggacagaa gcagcaatcg cctttcaatc
     9481 tggattcaac tgcaatatgg gcgctatctc tgcagtaatg acaaaagaag atgctatctt
     9541 atcggatgaa ttaaaccatg catctattat tgacggttgc cgcttatccg gagcaaaaat
     9601 catccgaatt aaacatcaag atatgaaaga ccttgagaaa aaagcaaagg aagcgataga
     9661 aagtaagaaa tataaaaaaa tcatgtatat cacagatggt gttttctcca tggatggaga
     9721 cattgcacga ttgccggaaa ttattccgat tgttgaaaaa tatggactga tcacctacgt
     9781 cgatgatgcg cacggctcag gcgtaactgg aaaaggtgca ggaaccgtca agcattttgg
     9841 attaagcgac aaaatcgata tgcaaatggg cacgttatcg aaagctatag gtgttgttgg
     9901 gggttatgta gctggatcga aaacactaat cgactggttg aaagctagaa gtcggccatt
     9961 cctattttct acttcgctaa ctccaggtgc agctgcagct gcattggctt cgattacttt
    10021 gatgcaagaa catccggaac ttgtcgaaaa agtttgggaa aatgctaatt attttaaaga
    10081 agaattgaaa aaagtaggct ataacatcgg tatgtctgaa acacctatta ctccagtcat
    10141 tttgggagac gaaaaagtga ctcagacttt ctctaagaag ctgatcgaac atgggattta
    10201 tgctaaacca atcgtttatc caactgtgcc tcttgggaca ggcaggatca gaaatatgcc
    10261 gactgcggaa catacgaggg aaatgcttga tgaagcagta gcagtttatc aaaaaatagg
    10321 aaaagaaatg gaaattattt aactaaattt agttttttcg tctatttaaa attagagaac
    10381 atgttcacgc atatattgtt aatcatctga agatagtagt aaaaaagatc agggaggatc
    10441 atcatgaaaa aagtattggt gacaggatgt ttaggacaaa ttggttcaga gttgatcaca
    10501 cggctacgtc aagaactagg cagtgaaaat gtcattgcga cagatatacg catgcctgaa
    10561 gtcattgaga aaaacgatat ctttgagatc ctagatgtaa tggattacga aaaaatgcgc
    10621 agcatagcag aacaatatca agtggacaca ttgattcatt tggctgctct tttgtcagct
    10681 gttgcagaag caaaaccaca attagcctgg aatttgaacg tgactgggct ggtcaatgca
    10741 ttggaagtcg ctagagaatt ccagctgaaa ttttttacgc caagttcaat cggcgcgttt
    10801 ggtccaaata ctccaaaaga tcatacccca caagatactc ttcagcgtcc tacaaccatg
    10861 tatggggtaa cgaaggtagc tggagaacta ctatgtgatt attattatgt gaaatatggc
    10921 gtagatactc gtggtgtccg tttccctgga ttgatttctt atgcaacctt gccaggaggc
    10981 ggtacgacag actacgcagt cgatatttat tattcagcaa tcaaagaagg aaagtacact
    11041 agtttcattg atcaaggtac atcaatggat atgatgtata tgccagatgc gatcgatgcc
    11101 attataaaac tcatgaatgc agatcctgat cgattggttc atcgaaatgc cttcaatata
    11161 tcggcgatgt cttttgaccc ggaagagatc aaagcatcta ttcaaaaaat cataccagat
    11221 tttgaaatgg attatgatgt ggatcctgta cgtcagtcaa tagcagaatc ttggccaaat
    11281 tcactagatg tttcttgtgc gcaagaggaa tggggctttt cgccgaaata cgacttagat
    11341 gtgatgacaa aggatatgtt ggaaaaactg aaagaaaaat tggtagagac ggtataaaac
    11401 aagaaccaca tccaaaaagt tttttatact tttgggattg gctatggtat gaaaggggac
    11461 aagggatgga agaatatcaa gccacgccag taaaagaagt aaaaatagaa aatgaattaa
    11521 aacgtacgat gggatttttt actgctttat cgactgttat gggcacagtg atcggtgcag
    11581 gggtattttt taaagccgca agcgtagcag aagttacagg atccgccagt ttgcatatgt
    11641 tttcttggtt tctcggtggt atgatttctg tctgtgcagg actaacagga gctgaactgg
    11701 cagctgctat tccagaaaca ggagggatga ttaaatatat cgaacggatt tacggaaata
    11761 cagcagcttt tttattgggc tgggcgcaag tcgtgattta ttttccagca aatgtagcag
    11821 ccttgtcgat cattttcggt acacagttcg tcaatctatt tggtctttcc cagtcaatga
    11881 ttgttcccgt agctgttaca gctgctgtat cgatcctgtt gatcaatttt ttaggttcta
    11941 aagctggagg ggcttttcag tcgattacgc ttgtctgcaa gcttattcct ttgtttgtga
    12001 tcgtcatttt cggtttgttc cgacaagaag gagtagactt ccaactgttt ccgatccaag
    12061 caggagaaaa tctttcattt ttctcagcct tgggagcggg cttgctagca acgatgttcg
    12121 catacgacgg ttggatacac gtaggaaata tttcgggaga attgaaaaag ccggcgaaag
    12181 atttgccaaa agcaatttct ttaggaatca tcggaatcat gatcgtttat ttattagtaa
    12241 atgcggtgtt cttaagaacc gcatctatcg acggagtcgc aggaaacagc aatgcggcaa
    12301 gtgacgttgc aaaaatgatt tttggcggct tcggtggaag actggtcaca gttggtatct
    12361 tgatttctgt ttatggaacc atcaatggct atacccttac tggaatgcgt ctcccttatg
    12421 ttatggcaaa agaaaacaac ctgcctttta gcaagctctt tgctaaactt catgataaaa
    12481 ctaaagttcc tgtagccgca ggtatcttag agctagtcat cgctattggc atgatgatga
    12541 tcggtggttt tgatacacta acggatatgc tgatctttgt catttggatt ttttatacga
    12601 tggtctttgt tggtgtcatt cttcttcgta aaaaagaacc tgatttgttt cgaccataca
    12661 aagttccaat gtatccgttt attccactag tagcaatcat tggagggact tttatcgtat
    12721 caagcacgct tatcactcag acctttttag cttcgatggg gatagtattg acacttgctg
    12781 gtatcccgat ttatctctat ttaaaacgta gatagctaaa acattcgaat tttattagaa
    12841 ctgcacttct ctgccaaaca aaatatttta tttaaatgag gagtacgtgg aagaaatgtc
    12901 aaaagtactc gttaattttg atcacgtaaa tgtcgttttg tctacgatga aatagcaaag
    12961 taaaagacag aaatcgggct caaccgattt ctgtctttta ctttgatttg cgtaactgaa
    13021 ctattcggcg tacgccgcga taaatattta ataaaaccaa gcaaccagaa atgaaaatgg
    13081 gaagccaaat cagatagaca gggtagccaa gcactttaga caaagcatgt ggagtcattt
    13141 tatccatttc aagaagtgct tgctgccaag tctgaagatt gaagactagc atgaaacccg
    13201 aaataatcag gagaataaat cctaaataat gaattgtagt tctttttctt acataagcga
    13261 tcgtagcata aaggaacagt aaaaaaatag gtacgccaaa aacaaagtac atattcagca
    13321 tctcgacacc ccctttgtgt ttatcttact ttactttgaa agaaatgaga aataatagcc
    13381 tcgttacttg cttgaaacag caaaggactg ctaagataag agagatacaa agaatcaagg
    13441 aggagtcata tggcaagtta tatcgaaccg attcgttttg cgctgattgt ttttccattt
    13501 ttagccttag cgatctcatc tgtttttttt atttatgaat atcgaaagta tggaacattt
    13561 ctagtaacaa gggcagttat tttgtattct ttcgtttttt atttattgtg tgcgtatttt
    13621 ttggtgatct taccacttcc ggcaaaggag acggttgccc aaatgacagg gccaaagatg
    13681 gagctgcatt tatttgcttc atggcaacat ttcagagaca acacggtatt ggtcttgtct
    13741 gaacctagta cctacctgcc ggcaatgaag cagagtgtat ttttggaacc agcttttaat
    13801 gttttattac tcataccatt tggcatttat ttaagatatt atttccggct ttcttttatg
    13861 aaaacgatga ttgctagttt ctgcctttct ctattttttg aattgacgca gctaagtgga
    13921 ctttatttta tctacccaag accataccgc ttatttgatg tgaatgattt agtgacaaat
    13981 acattgggtg gaatcattgg ttttgtcgtt acgccgttat ttacatttat gcttcctaca
    14041 agaagacgaa tggatgaact ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc
    14101 ttggtcgctt atttgatcga ctggacattt ttatctctta ttaatttggt agcggcgatt
    14161 ttctttaaaa ttgcgttcga ttcaggggca gttacttctc atatctggtg gcttttatta
    14221 gaagtagggg tatactttat gattgtaccg tttataacca atggatggac acttggcaaa
    14281 aaaattgtgc gtatccgttt ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg
    14341 atgatccgct acgggtatct gtactttctt ttttatggtt taagctggtt ggggcagttg
    14401 cttcagcaaa tcatgaattc gacaacgaat acggtgttgc agatgagtgc agtattcatt
    14461 tctctcggtg tcttgttact acaaggtatt tttattttaa atatcttgct atcatttttg
    14521 agaaaaaaac ggattctgtt ttatgaaaaa gctagtcaca catatactgt tagcacgatt
    14581 ggccaaacaa aggatgatga ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta
    14641 taatgccaaa gtcgtgataa tttaaagaga agaggtgcac gaatgaataa ttttgatata
    14701 ttccgcttca aaccgtttat caatgaagcg ttaaccgcaa agggcttcaa acagcccaca
    14761 gaggtccagg aacgtttgat tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct
    14821 caaactggta caggaaaaac acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca
    14881 gaaaaagagg aagtacaagt tgttattaca gctccaagta gggaactagc tgcacagatc
    14941 tatgaagcag ctacacaaat cgctaaattt tctacaccgg agatccgtgt ctcgaatttt
    15001 gtcggcggta cagacaaaca gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt
    15061 gtagtaggca cgcctggtag aatattagac atgatgaatg aacaagcgct gaagattcac
    15121 accgcatttg catttgttgt cgatgaagcg gatatgacat tggatatggg ttttttaaat
    15181 gaagtagacc aaattgcaag tcgattacca gaaaagctgc agtttttagt tttttctgcc
    15241 actattccag aaaaacttcg tccattctta cgaaaataca tggaaaatcc aatcattgaa
    15301 gaaatcaaac caaaagcagt tatttctgaa gacattgaaa attggctgat ttcaacaaaa
    15361 ggtaaagatg agaatcaaat gatttatcaa ctgctgacaa ttggtcatcc ttatttagca
    15421 atcgtatttg ccaatacaaa acagcatgtg gatgaaattg ccgattactt gaaaaaccaa
    15481 ggcttgaaag tagcaaagat tcatggagac atcaccccaa gagagagaaa acgagtgatg
    15541 cgacaagtac agaatctgga gtatcaatat gttgtagcga ctgatttagc tgctagaggg
    15601 atcgatatcg aaggtgtctc tcatgttatt aatgcagaaa ttccagaaga tctagacttc
    15661 tttatccatc gtgtcggacg tactggacgt aatggcttaa aaggaacggc cattacgtta
    15721 tattcgccaa atgatgagca agccattgat gcgatagaaa aattgggcgt ctcatttgaa
    15781 ccaaaagaaa tcaaaaacgg ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa
    15841 cgagaaaaat caaaagatgt attagatccc acgctgattg gtcttgtgaa gaagaaaaag
    15901 aaaaagatca agcctggtta taagaaaaaa atcgattggg caattgcaga aaaaaataaa
    15961 aaagaacgca aaatcgaacg cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat
    16021 tcacataaat aaaaaacaag aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa
    16081 gctagacttt ttttcattcc gatacaaagt acttcatctt caataaaagg ttctgtgcaa
    16141 gtatgatacc cttgtttttc ataaaaatct acagccgtca tttcagctac tacataacta
    16201 gtctgacaac cttctgaaac cgcccgtttt tcagcagctt caagcaactg accgcctaac
    16261 ccttttcctc ggtattcttt tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt
    16321 ttcttttcat aacgcaaagt agctactggt cttttttgat caagaaatag ataataggtt
    16381 tgttgcggga tatcttttcc gtcgaattct agttctgcag gaatcccttg ttctaataca
    16441 aaaacttgat atctcaaaaa gcagctagct gcgcgtatcc attcatccgc tccaactatt
    16501 tttttcatca tattcctcct tgtcatgaat agcagagatg tattgattcc tttattctta
    16561 tcgtaccaaa aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt gagatattga
    16621 cagtgatcaa tagaatgcgt atactaatta agtacgaaaa ggatatgatt tgttttttaa
    16681 gactcacaga aaattctttc ttggctgaaa aaagaatagt cactaatcaa attgctccct
    16741 ttttctattt tggtagaaat accgacgcag ctcagcgtta tcgagtttaa gaggtaatga
    16801 ttacacatca ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg tgatggtgtt
    16861 tttttgtatt taatttatag aaagcaggta atgacatgaa acaattaagt agtgcacaag
    16921 ttcgacaaat gtttcttgat ttcttccaat ctaaaggaca tacgattgaa cctagtgcgt
    16981 ctttgatccc cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt gcaacattga
    17041 aaaaatattt tgatggttca gttgtgccag aaaatccaag aatcacaaat gcccaaaaat
    17101 ctattcgaac aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg
    17161 agatgctggg taatttttcg attggtgact atttcaaaaa tgaagccatc cactgggcat
    17221 gggaattttt gactgatcca aaatggatgg cattcgatcc agaaaaactt tatgtaacag
    17281 tttatccaaa agatacagaa gcaaaacgca tctggcatga agaagttggc ctgccactag
    17341 atcacattat tgatgtagaa gacaacttct gggatatcgg tgcaggccca tgtggtccgg
    17401 atacggaaat tttctatgac cgtggagaag cttataacga tttagcagaa gacgatcctg
    17461 aaaactatcc aggaggagaa aatgaacgtt atttagaaat ctggaactta gtattttcag
    17521 aattcaacca taagcctgat aacacatttg aaccacttcc tcataaaaac attgatacgg
    17581 ggatgggatt ggaacgcatg gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag
    17641 acttgtttat gcctattatc cacgccgtag aaaagctaag caaacaagta aaatatggtg
    17701 aaaacccagt aacagatatt tcttacaaag taatcgcaga tcatattcgc gccctttcat
    17761 ttgctattgg cgacggtgca ttgccatcga atgaagggag agggtatgtc ttacgccgtt
    17821 tgcttcgtcg tgctgtcatg catgggaaaa aattaggtat tgaacaagca ttcttatatc
    17881 gtttagtccc tgttgttggg gaaatcatgg taagttatta tcctgaagta cttcaacaaa
    17941 aagaatttat tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa acaatcaatg
    18001 aaggtttgga aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac gggacgactc
    18061 tagcaggaaa agacatcttc aaactatacg acacatatgg cttcccggta gaattaacgg
    18121 aagaggtggc agaagatgaa ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg
    18181 atgcacaacg cgaacgtgct cgctctgccc gcagtactga aaaatcaatg ggggtacaat
    18241 ctgctttatt gacagatatc aaagtagaaa gtaaattcat tggatacaca gaacaagaat
    18301 cagatagtca attattggtc atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag
    18361 gtgaagctca aatgatcttt gatcaaacac ctttctatgc agaaatgggt gggcaagtag
    18421 cggatcaagg atggatcatg aatgaagtag gcgaaatcgt agcagttgtt acagatgttc
    18481 aaaaagcgcc taatggtcaa tttatgcaca ccgtagaagt aaaagcacca atgagtgaag
    18541 gacaaaccta tcatctattg atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata
    18601 cagcaaccca tctactgcac aaggcattga aagaagtact tggcgagcat gcaaatcaag
    18661 ctggttcttt agtcgctcct ggacatttac gattcgactt tacccacttt ggtcaaatca
    18721 cttcagatga attagctgaa atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc
    18781 cagttgaaac aattgaaaca gatatcgata cagccaaaaa catgggtgca atggctttgt
    18841 ttggtgaaaa atacggacat gacgttcgag tagtaaatat tggcggctgg tcaatcgaac
    18901 tgtgcggagg cacacatgta gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg
    18961 aatcaggaat tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg
    19021 aactacttga agaagaagaa aaacaactaa aacaagtagc aacaattgtt aaatcacctc
    19081 aattgaaaga agttgtttca aaagcggaac aattgcaaca gcaagtaaga gagctacaaa
    19141 aggaaaatga acaattagct agcaagttag cgaatcaaca agctggtgat gtattcaaag
    19201 atccagaaac gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg aaagatatga
    19261 atcagctgcg tcaattagct gaccaatgga aacaaaaaaa tatttcagat attcttgttt
    19321 tagcaaacga acaagaagga aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg
    19381 aagctggact aaaagctggc gacttgatta aagcaatcgc accgaaagta ggcggtggcg
    19441 gtggtggacg tccagacatg gcacaagctg gcgggaaaaa tcctgctggt attccagatg
    19501 cacttaaagc tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa
    19561 acaaaagttc aaacattgaa cgaaacatga ttatgggggc gaagacaaac aagtcttgcc
    19621 ctcatacttt tttcaattgg atggcgcatc taatcaaatg gtcggattcg gatggtcttt
    19681 agttgacgaa agtgacagaa atttttacga ttagtataaa taaaaagggg tgaaaaaatg
    19741 aatagtagaa ataagacctt tcgactagtc ttacgtgcga tcttgttagc aatcatcatc
    19801 gtacaagcga tggttccttg gctggggttt attccattag gattcattag tttaacaatt
    19861 atccatatca cagtaattat tgctgcagta gtattaggcc caaaagatgg catggtgatt
    19921 ggcttgtttt ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac aacgccattt
    19981 gatacattag tgttcacgaa tccgattatc tcggtagttc ccagagtact tgttggctta
    20041 gtagcaggct tggttttcca ctggatttat caaaaaaata aatcaataac gatcagctca
    20101 gcctttgcag gggtactggg ttcattggtc aatacagtct tagtgttagg attcatggga
    20161 ttattttata caggtgcaac ggcagatgcc tatggtgtag actctgctct tttatttaaa
    20221 accttagcag gggttgcagc aatcaatggg ataccagaag caataggtgc gggaatcata
    20281 acaccattac tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga ataatgacca
    20341 tcttttaata gtaataggac gcacgataga tagaaaaagg aagagaggag aggaaaacaa
    20401 tcgtcctctc catcttttta cctgagcaac aagcaaacta gaaggggtaa aaaacttggc
    20461 taagaaacaa gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa aaggagtact
    20521 agcgggtatc ttaggattgg ttacgaatct gatcttgttt gtctctaagc taatgatcgg
    20581 gcttttttca ggaagtgtct ccattatggc tgatgcagtg aacagtctat cagatactgc
    20641 ttcatccata ttgactttga tcggatttaa aattgctgca aagccagcag atcaggaaca
    20701 tccttatgga cacgaacgct tcgaatatat cagtgggtta tttgtttcga tcatcattac
    20761 ttatgtagga tttcaatttt tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt
    20821 ctctttaaca cccatcgtct tttttgtcct aatcatttcc atctttttga aatttgcaca
    20881 aggcaaaatg tatcaggtta ttgcgcaatc cattgattct gaaacattga gagctacagc
    20941 aacagatagt tataatgatg tctttactac actggctgta ttgctttcag cagcaatcga
    21001 atgggtaact ggatggcgta ttgatggata tataggtttt gctttagcat tatatatcct
    21061 ttatagtgga ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac
    21121 gccagcagag attgaagaaa tggaagagca tttaaatgct tgcgatgata tacttggcta
    21181 tcatgattta ttggtgcata actatggacc caataaacgc tttgcatcag tccacatcga
    21241 agtagatgaa tcaatgaatt tgaatgtggc tcataagatt attgatctga tagaaaaaga
    21301 ctttaaaaag tctttggatg tggaacttgt ctgtcacttg gatccaatgc ctgttgataa
    21361 cgaaaaatac tataatatct tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg
    21421 gcttaatatt catgatttta gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt
    21481 ggtccctgag aaattccagt ttgttgatac agaactagaa agaatcttac gtagtcaaat
    21541 agaagaaaaa atcggagatt accaattgga aattacgttt gatcacaatt atcttttata
    21601 ttaaatgaga aactataaaa agactcctta aagtttttaa tttaaagagt ctttttttct
    21661 tttaattgtt tatggagagc ctgacgtgca agattatctg ctcctttatt tttgttttcg
    21721 ggcaaccatt gcaggatcaa taaagaaaat tcggatagaa gttcttggat tctttgaagg
    21781 tagggaagga agagctcgtt cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg
    21841 ctgtctgaaa aacagaaaat cgtttcattc tgtaagtcgt ttttctttag aaattctaaa
    21901 gtttttaaaa agacttcgaa ctctgcttga tggtttgtcc ctatagaaag cgggatagat
    21961 agctgttctt gtttaccttg atagacaatc aagattccac ctccactagg accagggttg
    22021 ccttttgtag aggcatcaat gtatactttt aacataaaaa gagattctcc tttttgttca
    22081 aactaatgtt atggtaagat tatatcatta ctgaatcggt gagaggttag agaatgaaaa
    22141 aaaatattcg atggcagcct gaactggcac atacgatcat ctattggtca ttgaccttga
    22201 tcattctgtt ttttagtctg attctttctt tagaaaacac aggtccttat tggaaaagca
    22261 attttgtttt aggtattttt cttgtttttc tatttctagg tttgcggcgt tcttttattt
    22321 taaaatcaaa ctctgtgatt atacgttatg ctgctttttg gaaagaccga gaaatatctg
    22381 tcggacagat tcgatctttg acgccaacta atactggggt gcaaatgatg ttgcaagatg
    22441 agaaagcccc atctgttttc atgatgcgaa aaaaagatca agtagtcctt tttgatcatt
    22501 ttgaaaaaaa cggacccaaa tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa
    22561 gctaatttta aaaatcagaa cctaaaaaaa cgaattttta caggaaaaga aaagagtgag
    22621 acgtacggtt catcccgttt agcgtctcac tctatttgtt tatctattca tgcatactag
    22681 tatcggtagt ctgaacgttg cctttcttga catgggcagc ttgtgttcct cgattccctt
    22741 cgatgatctc aaattcgacc acttgatttt cttccaaagt tttgaatccc tcttcttcaa
    22801 tcgcagtaaa atgtacaaaa atttcttctg tgtcatcata actgataaag ccgtatcctt
    22861 ttttgttgtc aaaccatttt acaattcctg tggtcaaagc actcacgacc tttctaaagt
    22921 ctgatcatag ccttcattat aaagagtatt tataagtacg tcaaataaat taaaaacctg
    22981 taattttatg ttttttttct gaataaaaaa aggtataatt ggcatatctt aacaaagaag
    23041 atattttgtt gaataaagtt cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt
    23101 tcacaagaaa cagagctaga accgattgtt gaagtggcta gtaaattaga tcttaaggaa
    23161 gacgatttgg aactgtatgg gaaatataag gcgaaaatca atttttctgg aatcaatcga
    23221 tcaaaggata acgctgaggg gcatttgatc ttagttactt ccatcaatcc aacaccagct
    23281 ggcgaaggaa agtcaacgat cactgtaggg ttaggagacg cgctcaataa aatcaataaa
    23341 aaatctgtta ttgcattgcg agaaccctca cttggtcctg tgatgggaat caaaggtggt
    23401 gctgcaggcg gaggatatgc acaggtattg cctatggaag atatcaactt gcattttact
    23461 ggtgacatgc atgctatcac aacagctaat aatgcattat ctgctctttt agataatcat
    23521 attcatcaag gaaatgaatt gaacatcgat gcccgccgag tgatttggaa acgggtagtc
    23581 gatctcaatg atcgtgaact gcgtaaagta attgttggct tgggtgggcc aatccaaggt
    23641 gttccaagag aagatgggtt tgatatcaca gttgccagcg aaatcatggc tatcttgtgt
    23701 cttgcggcag accttgaaga tttaaaggct cgtcttgcaa ggatcgttgt cggctataca
    23761 tttgaccgtc agcctgtgac tgctggagac ttgaaagcag aaggcgctct ggcattactg
    23821 ctgaaagatg caatcaagcc gaatcttgta caaacaattt acggaacacc agcttttgtc
    23881 cacgggggac cgtttgccaa tatcgctcat ggctgcaata gtattttagc taccaagaca
    23941 gcattgagat tagcagatta cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga
    24001 gagaaatttt tagatatcaa agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt
    24061 gtcgccactg tccgtgcatt gaaaatgcat ggcggaatga aaaaagatga acttaaaaat
    24121 gagaatcttg atgctttgaa agtaggtttt gcaaatttaa agcgccatat acgtaatatg
    24181 gaacaatatc agctacctgt gatcgtcgca atcaatgaat tcgtaacaga tacagacaat
    24241 gaactcacac tgttggagca cttgtgtgaa aatcagggga ttcttgccaa gagagcgagt
    24301 gtatgggcag atggagcaga aggcggcgtt gatttagcaa aagctgtagt acgattgatt
    24361 gatagaaaag aagctaatta caagccatta taccgcctag aagaaacgat acaagaaaag
    24421 acagaaacaa tcgtcaagaa aatttacgga ggaaatggtg ttgttttcag caaaaaagcg
    24481 caaaaacaaa tagaagagtt tacgaagaat ggatgggaca aactgccgat ttgtatggca
    24541 aaaacacagt attcattttc tgatgatcct tcactgctag gtgcaccgga agatttcacc
    24601 atcacgatcc gcgaaatcat cccaaaatta ggggcaggat ttttagtagc cttgactgga
    24661 gatgttatga cgatgcctgg tttgcctaaa aaacctgctg cattgaatat ggacgtgaca
    24721 aatgacggag aagtgttggg attgttttaa gcttgattat aatctaccat cgaaggagct
    24781 gtattttgac tacagcttct ttttttagac gatagggaat taattttgtt tacactagga
    24841 tcattctttt tctttattgt attttgagct atactaaagc ataataggag ggtatgatga
    24901 aaacgacatt tataagaaaa accggttttt acggtatggg tagctcgatt cagctaagaa
    24961 tcaacaatga aaaacgcagt gttctcagtc atgaacaaaa gataacgctt gatctggaag
    25021 tgccctttac gatgcaggtc accttttatt ggctaaaaag ccccgtttat acagtgaaag
    25081 aagcaaagcc tgcttatgtg atcacaatga attacttgat tcttcaaatc tatccgtttt
    25141 tatttctagc aactgggttg ggggccgttt tcctacaaaa tttatttttc agtttttttg
    25201 cagttattgt gttgattgcg ttctttttct atataaaaaa ccgggcatat ttgatcaagg
    25261 aggaaaatta tggcaaactc tgatcttttt ttaatcagtt tcaatcgaat cgaaaaatgg
    25321 atgagagacg agatgggaaa tgcacgaaat atgggattta ccgaacttgt cagacgccta
    25381 gctcaaagaa aacaattaat gatcagaaaa tacgaagatg atcttttaca gcttgctcag
    25441 ttaaggaatg cgatcgtcca tgatcggatc gcagtcgatt ttgtcattgc agagccaaat
    25501 gattggacag tcaagcgaat ccagctgatc gaacaagaac tgattcagcc tgaaaccgtg
    25561 ctgcctcgat ttgcaaaacg tgtaacaggt tttgaacaag atcttccgtt acttgaactg
    25621 ttaaagatag tagcagaaaa aaggtattca caatttccgt tatacaataa aggaaaattt
    25681 gtagcattga ttactttacg taatatcggg ttttggctag caaaagaaag ccaaaaaggt
    25741 ccggttgatt taacaaataa acgagcgttg gatttgatca tccaaaacgg aaagtacaca
    25801 aattatcatt ttgttccagc taaaacacat atttacgagg ttgaagcgat gtttcgtgag
    25861 caagggactt tggaagccgt acttatcact aaagatggga atcccgatgg taatctgctc
    25921 gggatcgtac gacctcgtga tatttataaa caaatagaaa aggattgatt ttttgttaat
    25981 tatttattgg atattcagcg ctttgctagt aggagtggat cagttggtca aatggtggat
    26041 cactgataat ctagcgcttg gagagacgaa aaatcttata ccaggtattt tgtcactgaa
    26101 tcatattcga aacactggag ccgcatggag tatgctagaa gggaagatgt ggttctttac
    26161 tatagtgaca ctcattgcag tcgttgtgat tctcacattg atgattaaaa atcgtgaaaa
    26221 aggtaatcgc tggttcatga ttggattgag tttgattcta gctggtgcga ttggcaattt
    26281 tatcgaccgt attcgtcttg gctatgtagt agatatgttc cagacagatt ttatgagctt
    26341 tccgattttt aacgtagcag atgtgacact tgtatgtgga gtgatttgta tgttaattta
    26401 tattatttta gatgaaaagg atcaaagaaa aaaatgacag aacttaaaac gacgattcac
    26461 catgaatctg gtaggatcga taaagtattg actaatttat tcaccgatta cagtcgttcg
    26521 caaattcagc tgtggctgaa aaatggagcg gtttctgtca atggacagcc agtcaaagca
    26581 aattacaaag taaaacaagg agacgagatt actgtcttga tccccgaacc agaaacatta
    26641 gatgtggtcc cagaaaatat cccgttgacg atcgtttacg aagatgaaga tgtagcggtg
    26701 ataaacaaac ctcaaggaat ggtcgttcat ccttcagcag gtcatacgca tggcacaatg
    26761 gtcaatgcgc tgatgtacca tatcaaggat ctttctacga tcaacggcgt tattcgtcca
    26821 ggtattgttc accgaatcga caaagatact tccggactgc taatggttgc caaaaatgac
    26881 caggcacacg aatcattagc tcagcaatta aaagataaga cctctttgcg caaatatgtt
    26941 gctttagtcc atggagagat tcctcacgaa aaaggaacca tcaatgcgcc aatcggccgt
    27001 tcgaaggaaa accggaaaat gcaggcgata agagaagatg ggaagtcggc tgtcacacat
    27061 tttactgtat tggaacgttt cgaaggattt actctggttg aattaaaact agaaaccgga
    27121 agaacacacc agatccgtgt gcatatgaag tatatcggtt atccgttagc tggagatcca
    27181 gtatacggac cgaagaaaac tttgaaaggc aatggtcagt ttctccatgc gcaaatgtta
    27241 ggttttacgc acccgactac aaacgaaaag atgatctttg aagcacctct tccagaagtt
    27301 tttgaaaaaa ctttagaaaa attaagaaaa aacattgcat tttaatttag ataacggtat
    27361 agtaattaac aacgaaagca taaaacttaa tagaattcct ttaagtttag tcccgtgagg
    27421 ctaagaaggg tgggagcaag gatacgtatg gtgcgccata agtagattgc tcattgacat
    27481 agtatagcta tcagtgtatc agtatacgct cagtctaaac attcctcctg tcctcttagg
    27541 gcaggagttt ttttatgcaa aaaagtaggg ggaatgaaaa tgcaagcaaa agaagtagta
    27601 gatcaggtaa ccatgaagcg agcattgact cgaattactt atgagattat cgagagaaac
    27661 catagtatcc aagatatcgt attggtaggt atcaaaacac gaggaatcta cattgcatca
    27721 agaattgccg aacgcctgaa gcagctagaa gatatcgata ttcctgtagg agaactggat
    27781 atcactttat accgagatga caaaaaagaa aatccagaag aaccggaact tcattcttct
    27841 gacatcccag tttccttaga aggaaaagaa gttattttga ttgatgatgt tttgtataca
    27901 ggacgaacga ttcgagcagc aatggacgca gtaatggatt ttggcagacc aagaaaaatt
    27961 tcattagcag tcctggtcga tcgtgggcat cgagaattgc caatccgtgc agattatgtt
    28021 gggaagaaca ttccaacatc tcgggcggaa gagattttag tagagatgca agagcttgat
    28081 ggacaagatc gcatcatgat tttgaaagag gaagattaga ggagagagaa acatgtcgga
    28141 gaaaaaagga tttcataatg atgacgtggt gttagatatc cacgatcgcc caaccccagg
    28201 acattgggta ggattaagtt tacaacactt gttcacgatg ttcggagcaa ccgtgttagt
    28261 ccccatctta gtaggtatcg atccaggtat tgcgctagtc agttcaggac taggtacatt
    28321 ggtgtactta tttactacta aaggaaagat acctgcgtac ttaggaagca gcttcgcttt
    28381 cattgcagcg atgcagcttt tgatgaaaac agatggttat ccagcgattg cacaaggagc
    28441 agtcacaact ggattagtct atttgattgt tgcgctgatc atcaaaaaga tcggttcagc
    28501 atggctggat aaaatcttgc ctccaatcgt cgtcggtcca gtcatcatgg ttatcgggtt
    28561 gggacttgcg gcaaatgcag caacaaatgc tatgtataat cagggcacgt atgatttcaa
    28621 gtatattgct gtagcgctga tcacattagg attaacaatt ttcttcaaca tgtggctaag
    28681 aggttttctc ggattgattc cgattttact aggtattatt agcggataca tcgtagcctt
    28741 gctgtttgga atcgtggata ctaagccaat cgtagacgct gcctggtttg ctgttcctaa
    28801 ctttgagatt ccctttgtac aatatgaacc aaagctatat cttggagcaa tcacaacaat
    28861 ggcacccatc gcttttgtga cgatgacaga gcatatcggc catcttatgg tattgaataa
    28921 actaactaaa cgtaatttct ttgaagaacc gggtcttcat aagacgttga caggtgatgg
    28981 attagcacaa atcgtggcag cctttgtcgg aggtcctcct gtaacaagtt acggtgaaaa
    29041 cattggtgtt ctggctatca cacgtgtcca tagcgtgttt gtgatcggtg gagctgcagt
    29101 atttgcagtc attttaggat tcgtaggaaa attaagcgct ttgattttaa gtatccctgg
    29161 accagtcatc tcaggaatca gttttatctt atttggagta attgccgcaa gtggaatgaa
    29221 gattttaatc gagaacaaaa tcgattttga taaaaagaaa aatctgttga ttgcttcagt
    29281 tatcttagta gtaggaattg gcggactcgt ctttgaagta ggcacattta cattgtcggc
    29341 aatggcatta gcaactgtac ttggcatcat tttaaatctt atcttaccag aaacttcacg
    29401 aagcgaagaa aactaaggag gaaacattat gatcatcaaa tcagaacgta tcagcttaaa
    29461 gcatttattg accgttgagg cacttactga tcaagaagtt atgggcttga tccgcagagg
    29521 gcaggaattc aagcaaggtg ctaattggac tccgcaaaag cgccagtact ttgctacaaa
    29581 cctctttttc gaaaatagta ctcgcacaca taaaagcttc gatgtagcgg agaaaaaact
    29641 agggctagaa gtgattgaat ttgaagcaag cacaagctct gtccaaaaag gggaaacact
    29701 atacgataca gtactgacga tgtcagcatt aggcgtagac gtagcggtta tccgtcatgg
    29761 tgatgagaac tattatgacg aactgataca aagtaaaaca atccagtgtt cgattatcaa
    29821 tggaggagac ggaagcggac agcatccaac ccaatgcttg cttgatttga tgaccattta
    29881 tgaagaattt ggtcattttg aaggtctgaa agttgccatc gtaggtgata tcactcactc
    29941 tcgcgtagct aaatccaata tgcagatgtt aaaacgtcta ggcgcacaag tattcttctc
    30001 aggtcctaga gaatggtacg acgatgaatt cgaagtgtac ggacattata tgccattgga
    30061 tgaactacta gatcaagttg atgtcatgat gatgcttcgg gtgcaacacg aacgccatga
    30121 tggaaaagaa agtttctcaa aagaaggcta ccatcaggaa catgggttaa cagttgagcg
    30181 tgcgaagaag ttgcaaaaac atgcaatcat tatgcaccca gcaccagtta accgtgatgt
    30241 agaattagct gattcattag tggaaggatt acagtcgcgg atcgttcagc aaatgagcaa
    30301 tggagtattc gtcagaatgg caatcttaga agcagtgcta aatggaaaag cgtaattata
    30361 ggaggatgaa aaaatgaaaa cactcattaa aaacggtcag atcaatacac gaaaaaatat
    30421 gacgacacca gcagaaattt ggatagaaga tgggcgaatc aaagcaatcg gtacaggatt
    30481 ctcagaagcc gaatttgatg aagtgtttga tgcaaaggga caattgatca cacctggttt
    30541 agtggacgtc catgttcatt tgagagagcc aggatttaca tacaaggaga cgatcgaagc
    30601 tgggactcgc tcagctgctc gtggcggttt cactactgta tgcgcgatgc cgaatttaaa
    30661 tcctgtacct gatacggcag aaaagctgag acaagtatat gacatcatta gaaaagatgc
    30721 agttgtgaag gtattgcagt atgcgccaat cacagaaaat ctgcgaagtg agaaattagt
    30781 tgatcaagaa gcactgattg aagaaggtgc ttttgctttc acaaatgacg gtgtaggtgt
    30841 ccaaacagct gggacgatgt atctggctat gaaagaagct gcaaaaaaca ataaagccct
    30901 agtagcccat acagaagatg aatctttatt atttggcggc gttatgcacg ctggcaagaa
    30961 ggcagaagaa cttggtcttc ccgggatctt aagcgtaacc gagtcttctc aaattgctag
    31021 agatctgttg ctagctgaag caactggtgt tcattaccat gtttgccatg tttctactaa
    31081 agaaagtgtc cgtgtaatca gagacgcaaa aaaagcagga atccacgtaa cagcagaagt
    31141 ttcaccccat catcttattt tgatcgacga agatattccc gaagatttcg gcttttggaa
    31201 aatgaacccg ccgctaagag gacgggagga cagagaagct ttgatcgaag gcttgcttga
    31261 cggtacgatc gattgcatcg ctacagacca tgccccccat ggattagaag aaaaaagcca
    31321 aagtttcatg aaatcaccat ttggtatcgt aggaagcgaa acagcgtttc aactgatcta
    31381 tactcatttt gtagaaactg gtcgattcac gttggaacaa gtgatcaatt ggttggcagt
    31441 taaaccggct gagatttttg gtttgaatgc aggaacgcta acggttggtg cccccgctga
    31501 tgtagcagtt tttgatatta cacaaacttg cacgatcgat aaagaagact ttttatcaaa
    31561 aggggagaat acacctttta tcggctggaa agtaaaaggc gaaacacaaa tgacttttgt
    31621 caacggaaaa cttgtgtggc agaagggaga ataaaagtat ggaacgtttg cttatattag
    31681 aagatggaac agttttcgaa ggaaaggctt ttggtgcaga tgctagtgtc ttcggagaaa
    31741 tcgtttttac aacaagtatg actggttatc aagaaactat cactgatcaa agttttaacg
    31801 gtcaaatcat tacatttacc tatcctatgg tgggtaatta tggggtaaat agagatgatt
    31861 atgaatcgat ttctccaaca tgcaaaggag tagtagtaaa agaacatgca cgtgtagctt
    31921 caaactggcg aaaccaaatg acgctagatt catttttgaa acaaaaagga attcctggaa
    31981 tatcaggtat cgatacgcgc gcccttactc gcaaaatccg tgaagtcgga acgatgaaag
    32041 ctagtatagt tgatgctgga gattccttcg aacatgcatt tgatcagctg aaagcaaccg
    32101 ttctagcaac caatcaagtg cagcaagtct caactaacaa accttatcca agtccaggaa
    32161 caggtcggaa agtcattgtc attgacttcg gcttgaaaca tagtatttta cgagaattga
    32221 gtaaaagaaa ctgtaacctc acagtacttc cctataacac agatgccgag acgattttag
    32281 cgctatcacc agacggagtc atgctgacga atggacctgg tgatccaaaa gatgtgccag
    32341 aagcaatcga aatgatcaaa aaaatccaag gaaaagtacc gatttttggt atttgtctcg
    32401 gtcaccagtt atttgcttta gccaacggtg cagataccta taaaatgaaa ttcggacatc
    32461 gaggattaaa tcacccggta cgagaagtag cgaccggaag aattgatttt acttctcaaa
    32521 atcatggcta tgcagtcgat gaagccacga ttgatccaga aaaactcatc gtaactcatg
    32581 tagaagtaaa tgacggaaca gtagaaggag tcaaacacag agattatccc gctttcagtg
    32641 tgcagtttca tccagatgct gcccctggtc cgcatgatgc actacattta tttgatgaat
    32701 ttatggaaat gatggacgca ggaaaggagc agtaaacaga tgccgaaacg tacagatatc
    32761 aagaaaatta tggtgattgg atctggtccg atcatcattg gtcaagccgc agaatttgac
    32821 tacgcaggaa cacaagcctg ccttgcctta aaagaagaag gatatgaagt ggttttagta
    32881 aattctaatc ctgcgacgat catgacggat aaagaagtgg cagatcaagt atatatcgaa
    32941 ccgatcacac ttgaatttgt atcaaggatt cttcgaaaag agcgtccaga tgctctcctt
    33001 ccaactttag gcggacagac aggcctcaat atggcaatgg agctggcagc atcaggtatt
    33061 ttagaagaat tgaacataga acttctagga acaaagcttt ctgcgatcga tcaagcagaa
    33121 gatcgtgatc tattcaaaca gttgatgaaa gaattgaatc agccaatccc cgagtctgaa
    33181 atcgtcaata cagtagaaga agctgtagat tttgcaaata aaatcggcta tcctgtcatc
    33241 gttcgtccag cctttacttt aggcggaact ggtggaggta tgtgtgaaaa tgaagaagaa
    33301 ttacgtatcg tatcagaaaa tggattaagt ctttcccccg ctacccaatg tttgattgaa
    33361 cgaagtattg caggttttaa agaaattgaa tatgaagtga tgagagattc ggctgataat
    33421 gcaatcgttg tctgcaatat ggaaaatttt gacccggtgg gtatccatac aggtgattct
    33481 atcgtatttg cccctagtca aacattgtct gaccatgaat atcaaatgct tcgtgatgct
    33541 tctctttcga ttattcgagc acttaagatc gagggaggct gtaatgtcca actcgctctt
    33601 gatccacata gttttaatta ttatgtgatt gaagtcaatc cgcgtgtttc tcgttcatct
    33661 gcgttagcaa gtaaggcgac tggctatccg atcgctaagc tagctgctaa aatagcgctt
    33721 ggattaacat tagacgaaat gaaaaatcct gttacaggaa cgacttttgc agaatttgaa
    33781 cctgcattag attatgttgt agctaaaatt cctcgctggc cgtttgataa gtttgaaaac
    33841 ggagaacggc ttttaggcac gcagatgaaa gcaactggtg aagtgatggc tatcggtcgg
    33901 aatatcgaag agtctttatt gaaagctgtg cgttctttag aaatcggtac acaccatatc
    33961 gaacttcctg agttggcaga catcacggac gatgacctaa tccaaaaagt ggttcgagca
    34021 caagatgatc gcctgtttta tgtagctgaa gctattagaa gagggtacac catagaagaa
    34081 ttagcggatt tgacaaaaat cgatttattc tttttagata aactgttgca tatcgtagaa
    34141 atcgaacagc agttgaaaca gcagccgttg gatgacaaga tattgaaaac agcgaaacaa
    34201 aacggtttta cagacaaaaa agtggcggag ctgtgggaaa taaacgaaga agccgttcgc
    34261 cagatgagaa aagaaaatga tttgattcct gtatataaga tggttgacac ttgtgcagcg
    34321 gaattcgaat caaaaacacc ttatttttat agttcctatg aatatgaaaa tgaaagttca
    34381 cgatcagaaa agccctcaat tctagtactg ggatctggtc ctatccgtat cggacaaggg
    34441 gttgaatttg actatgcgac tgtgcattct gtcaaagcaa tccagcaggc aggatacgaa
    34501 gcaatcatca tgaacagcaa tcctgaaaca gtttcgactg acttttctat ttcggataaa
    34561 ttatattttg agccattgac cttcgaagat gtgatgaatg tgatcgagct agaacagccg
    34621 ataggagtaa tcgttcaatt tggtggtcag acagcaatca acttagccga acctttgact
    34681 gctgctggtg taaaaatctt agggacaacg atcgaagact tggatcgtgc ggaaaatcgt
    34741 gatttattcg agcaagcgct gaaagagttg gatattcctc aacctttagg ggatactgct
    34801 acaagtaaag aagaagccgt ggttatcgct tcgagaattg gttatccagt tcttgttcgt
    34861 ccaagctatg tattaggcgg acgtgcgatg gaaatcgttg aaaaccagcg agatttagaa
    34921 gattacatgg aacatgctgt gaaagcatct ccagaacatc cagtactagt ggaccgttac
    34981 ttgcttggtg gtgaatgcga agtggatgcg atttgtgacg gtgaaacagt actcatccca
    35041 gggatcatgg agcatattga gcgtgctggt gtccattcag gggattcaat ggctgtgtat
    35101 ccacctcaaa cattatctga atcgatcaag aaaacgatcg aagattacac gatccgctta
    35161 gcattaggtt tgaattgtgt cgggatgatg aatatccagt ttgtcatcca tgaaaatgag
    35221 gtttatgtga tcgaagtcaa tcctcgagca agtcggactg ttccattctt aagtaaggtg
    35281 acaggaattc caatggcaca aattgcgacg aaagctattt tgggagagaa actaaagaca
    35341 cttggttatg aaaacggact ttatccagaa acaaaagaag ttcatgttaa agcacctgtc
    35401 ttttctttta cgaaattgca gaaagttgat acttacttag ggcctgaaat gaaatcgact
    35461 ggagaagtaa tgggatctga cagacaatta gacaaagctt tgtacaaggc attcgaagct
    35521 tctggattac acttgcctga atatggagcc gtattattta cgattgcgga cgaaacaaaa
    35581 gaagaagcac tagaattagc taaacgtttt acagaaattg gctatagtct gataggaacg
    35641 aaacatacag ctgcttattt cgaaaaaaat ggtctggtcg ttgaaacagt agcgaaaatt
    35701 tctgaggaag cagcagaaaa aaatgtagtc gacttaataa gagatggtaa aacacaagtg
    35761 gttgtcaata cgattgataa agatcgggga aatgcttcta aagacggttt catcatccga
    35821 agagaagcag ttgaacatgg tattccattg tttacttctt tagacacagc agatgcgatc
    35881 gttcgagtga tggaatcccg tgctttttca actaacccta tttaacggcg agcacatgtt
    35941 aagacaaaaa gacgaaaaac cgttgcagga ggggacagat gaaacaagaa atgatggaaa
    36001 tcgttcagca aaaacagctg gcaccaaaga tttttgaaat gacattgaaa ggtgatctag
    36061 taaaagaaat gcagcaacca ggacagttct tgcatattct cgtgccgcgt gcagacttgc
    36121 tattacgccg accgattagc ttgaatcata tcgaccacga aacaaatact tgccgtatca
    36181 tttatcgggt ggaaggtgag gggactagag tcttctctac tctttctgcc ggagaccatt
    36241 tagatgtcat gggtccttta ggcaatggtt ttgatttgtc caatcttcat acaggagacg
    36301 aagtatttat aatcggtgga ggaatcggtg tccctcctct atatgagtta tcaaaacagt
    36361 tagtcgaaaa aggaattaag ccaacgcatt ttttaggttt tgccacacat gaagtcgttt
    36421 attatgaaaa agaatttaat cgtctaggag aaacaagaat cgctactgac gatggaacat
    36481 acggtatgca cggaaatgtc ggtaatttat tgttaggaac gaagacggaa cctacagctg
    36541 tttttgcatg tggttcaaac gggttattga agacagtgga acagctgttt tcttcgcatc
    36601 aaaatgtcca gttatcatta gaatcccgca tggcatgtgg gataggggct tgctatgctt
    36661 gcgtttgtca tacgccggaa gatgaaacag gaacaaaaag tgtgaaagtt tgtgacgaag
    36721 gaccgatttt taaaatagga gaggtaatta tatgaatgag aatcctttag cggtcaaact
    36781 tcctggccta gaattaaaaa atcctatcat gccggcaagc ggctgtttcg gttttggcaa
    36841 agaatacgga aaatactatg atttgaatca gcttggatcg atcatggtca aggcaacgac
    36901 tccaaatgcc cgctttggca atcccacgcc aagagtggcg gaaacaccat ctggcatgtt
    36961 gaatgcaatt ggtttgcaaa atcctggtct agacgttgtt atgcataaac ttcttccaga
    37021 attagaggag tacaatgagt tacccatcat cgcaaatgtt gctggtgctt gtgaagaaga
    37081 ctatgtagaa gtttgtagca aaattggcga agcaccaaat gtgaaagcaa tcgagctgaa
    37141 catttcttgc ccaaatgtca aacatggcgg aattgctttt ggaacagatt ctgatgttgc
    37201 ttttcaatta acacaagcag taaagaaagt ttcttctgtt cctgtctatg tcaaattgtc
    37261 cccaaatgta acagacatcg ttccaatcgc tcaggcaatc gaagctggag gggcagatgg
    37321 gtttacgatg atcaatactc ttcttggtat gcggattgac cttaagacaa gaaaaccgat
    37381 tttagccaat caaacaggtg gactctccgg accagcaatc aagcctgtcg ctattcgttt
    37441 gattcaccaa gtagcaagta tatccagcct tccaatcatt gggatgggcg gcgtgcagac
    37501 agtggatgat gttcttgaga tgtttatggc tggagcaagt gctgtagcta tagggaccgc
    37561 gaatttcacc gatccgtata tctgtcctaa actaattaaa gagctccctg tgcgaatgag
    37621 cgaactaggc atcgaatcgc tggaaagatt aatcaaagaa gtgagagagg aacgtgaacg
    37681 atgaatcaac tagaccaacg accaattatt gcattagatt tttcaactag gcaagaggta
    37741 gaagactttc ttcgattttt cccgaaagaa gaaaaattgt ttgtgaaaat cggaatggaa
    37801 ttgttctacc aagaaggacc tgaaatcgta cgttatctaa aagaggctgg tcataatgta
    37861 tttttagatc tgaaacttca cgatattccg aatacggtcg aaaaagcaat gcggggattg
    37921 gcaaagcttg gtgttgatct cacctgtgtt catgccgctg gcggtattcg tatgatggag
    37981 gcggcaatgc gaggattaga agaggggact ccagaaggtg gaaaacgtcc gctacttcta
    38041 gctatcactc agcttacttc tacaagtgaa gaagaaatgc atgccgatca actaatcgaa
    38101 gtttctttag aaaaaagtgt gatccattat gctagctgtg ccaaaaaagc aggattagat
    38161 ggtgtggttt cttctgcttg ggaagtagaa gcgatcaaag aaatggcagg agacgaattt
    38221 gtttgcttaa ctccgggtat aagaccagaa ggaacagtcg caggagatca gacaagagta
    38281 gtcacacctt ctcaggcaaa aaagatcggc tcaactttta tcgttatagg tcgaccaatc
    38341 actcagtcaa ctaatcctta taaagcatat caaacgattc aaacagaatg gagctaacct
    38401 aaaatgaatg ttgaacaaag tatcgcaaaa gacttattag aaattgaagc agtattttta
    38461 aatccaagta atccttttac atgggcaagt ggaatcaaaa gcccaattta ctgtgataac
    38521 cgaatcacga tgagctatcc aaaagtccga aaagagatcg caaaaggatt agcaagcaaa
    38581 atcaaagaag cttaccctga agtacaagtg attgctggaa ctgcaacagc aggaatccct
    38641 catgccgcat gggtagcgga aatactggat ttgccaatgg tctatattcg aagtaaggcg
    38701 aaagatcatg ggaaaggtaa ccaaatcgaa ggacgtatat ttgaaggtca gaagatggtt
    38761 gtgatcgagg atttgatttc tacaggcgga agtgtcttgg aagcagctga agcagcaaaa
    38821 agagaaggtg cggatatcct tggtgttgcg gctatcttta cgtatgagtt gccaaaagga
    38881 aaagcaaatt tcgaaaaagc cgaaatccct cttatgacgt tgaccaatta ttctgtattg
    38941 attgaagcag ctttagaaga tcgttatatc gatgagcaag aattaactct gctaaaagaa
    39001 tggaaaaaag atcctgaaaa ttggcagatt taagcgcgct tgatgaagat agagaaatcc
    39061 gatttgtgtc tccagaatca tcaattggaa tagcaaaaat ccaaaaactg attgcgagaa
    39121 gcatcttttc atcaggtatg aaaagaaact tctatcgtga tcagtttttg atttttttaa
    39181 aattcatttt tctaagactg gttagcagaa agaagagaat aatctcttct cttttttcta
    39241 ttatatatag attttctcta ttttttggta agggagctgt ttcaaaaatg gtaaagagcg
    39301 gaaatgttgc gaatatcagt tatttaatga tatgatattc ggaaaaagaa atctcaaagg
    39361 gaggtaactt ttatggggtt gaatgaattt attttaggat tgaacaattt agagacgatc
    39421 acaagggcac ctggattttt taaatataca gagcatacag ttgcagcaca ttcgtatcgt
    39481 gtagcatcta ttgcgcaagt tctaggagac attgaagaag cgaatggaca accgatcgat
    39541 tggaaactgc tctatgaaaa atcattgaat catgactaca cagagcgttt tattggtgat
    39601 atcaaaacgc cggtgaaata cgcaagcaaa gagttaagag ggatgctgca aacagttgaa
    39661 gaaaagatga cggacgaatt catcaaccaa gagattccag ctgagttcca agaagtttat
    39721 cgccgtcggt tgttcgaagg aaaagatgaa accgtagaag gagaactatt agccatttca
    39781 gataaggttg accttcttta tgaatctttt gatgaaatca tcaaaaataa cccggaaaaa
    39841 gtttataagg agatgttcat tgaggcagtg actacgatta aaagctttga acatcggcct
    39901 tctgttcagt atttctttaa agagatattt ccggaactat tgaaccagga attttatgga
    39961 aaagaggcgt ttttagaaac cgtctcaacg attgtaaaga aagaagaaaa ataaaaatga
    40021 tatgagatgc agggggaagt atagttgaaa aagaatatgg atgtcgaatg gggctattct
    40081 ggagacaaag gtccggaaaa ttgggcgagt ttatgtgatt ggtttgcgag aggtgcggaa
    40141 tttccattgc agtcgcctat tcatttgacc aagggggaag aaaccaaaat agaaggagac
    40201 acgctttcct ttcattatca aaagcaacgg tttactgaca aggaattcaa aaacacgatt
    40261 catttagtac cttttgacca actcagttat gtagagttca agaaagaacg ctattatttg
    40321 acagatattc atttccactt gccttctgaa catatcatca acggaaaaca ggaagatatt
    40381 gaatttcatt ttgttcatat gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac
    40441 cagctaatgg tacaggaagg ttggctgtat aatcaagaaa atggggaaag ttggaactta
    40501 gaaaaacatg agcattgggt aaatccggat gtgttctttc cggagcaaaa aagccatttc
    40561 cattatattg gctcattgac aactccgcct accagcgggc caatccaatg gtttgtaatg
    40621 gatcatgtag gaaaactgaa ccaagagttt cttctcccct tcgatggtca gtatgctcga
    40681 ccgaacaacc gaccaatcca gcccttgaat ggaagagaaa tttattattt cgaggagttt
    40741 gagcaatgaa tattcagcag atgaaatacg tggtcgctgt agcaaacaat ggtagtttca
    40801 gagaagcggc aaagaaaatg tttgtctccc agccaagtct ttcagctggt atcaaagaat
    40861 tagaaaccga gttagggatc acgctgttta ctcgaacgaa tcgaggggca tatttaacag
    40921 aagaagggca agacttctta aatcgtgccg agaaaatatt ggtccagctt gatacattag
    40981 aaactcacta tctaacgagt gaaaagagag acaatttttc gatcgctgcc cagcattatg
    41041 atttcttagg tcctttgacc gcacagctac tcgaggaatt caaagacgag atcaaacaat
    41101 ttcggatcat ggagacgaca acagcgagag tgatcgaaga agtcaaagag cagcacagtg
    41161 agatcggcat tatttatatg aatgagcata atcaagctgg tatcagaaga tatttacaac
    41221 aaggagagct aagtggccgc agcattggaa aattcaaaac gcatattttt ttaaggcaag
    41281 gacatccatt agctgaacac gcagagatca ccaaagaaga attgttatct tatcctcaag
    41341 ttcgttttac acaggatgga aataatttcc cgtattttta tgaagacctt atcgagatcc
    41401 ctgatcaaga aacagtgatt tatacgagtg atcgaggtac tttgatgaat atcgttcttg
    41461 aaactgatgc ttacgcttca ggatctggta ttgtgattgg agggattcgc gagcacttgc
    41521 ggttaatccc tttggcagaa ggcgaactaa atgaatttta tatcatttat tcggcgaaac
    41581 gtccgctcag tgaaatcgct caaagattta tcaaagagct tactcggtta ctggaccagc
    41641 agagctaaga aaagcagatg agaacgctgt ttattgaaca ccgttgatca aaaaagaggg
    41701 gttgtgacag aattttgtca caactcctct ttttgtagca aacaatttat aactaattgc
    41761 tgttgtggga aagtacgcaa atatgattct agaaacactc attgattacc tgttttgttt
    41821 catagctgta attttttctt cttctggtgt cacgataatc gtcttttggt tttcataaat
    41881 aacataacca ggcttggcgc catttggttt tcggatatgc tttacctgta caagatcaac
    41941 cggcacttgg gcagagtatc ggtattttga aaaataggca gctaactcgg cagcttctgt
    42001 aattgtttca tctgagggct tgtcactttt aatgatgacg tgcgaaccgg gtatattttt
    42061 tgcatgaagc caatagtcgg tttttttagc tgtcttcatt gtcagctggt cattttgaag
    42121 attgttttta ccaacaagaa tgagcgttcc gtcagaagaa aagtattgat caggctgaga
    42181 tggtttctta cgtttttgtt tttttaagtt cttctttttc aggtatcctt ctgaagtcaa
    42241 ttcttcttta attgcttcga tatccattgg gccggcaatt tccagttgag ataagacaga
    42301 ttcaagatat tggatttcat ctttggcttc ttggatttgt tcgccaatca gcttaacagc
    42361 gttcttcagc ttttggtatc gatggaaata tttctgtgca ttttgatttg gtgtcaatgc
    42421 cggatctagt gctattttga ttggccgatc ttcttcataa taattaggta aaacaacttc
    42481 ttttgcgccg cgtggtactt gtgtcagaaa agtagtaagt agttcgccat ttctacggta
    42541 attctcagcg ttttcggatt ctttgagtgt ttgttcacgt tttttcaatt tatttttatt
    42601 tcgttttagt tcattttcga tttttcgaat cagttctccg ccttgttgtt ttgcccgatc
    42661 tttctctgct ttttcgtgat aatagctatc taataattga ctgagtgttg gataagctgt
    42721 tacagcagat gcctgttcgg acaatacctg ataagcaatc ggtgtgaaaa aatctttatt
    42781 ttccgtttcg taaaaagtag gaataggctg atggctgatg gctgagaaaa attcgtccca
    42841 tacaaccatc ttttcattag gacgttccag taatcttttg gtcagctctt gagcgatatc
    42901 gaaaccgatt ccctgaaact gacgttggat tgcttttgga tctagttcag tttgggaaag
    42961 ccgctgaaag attttttctt tctcggagct aaatgggttc aagacctctt gttttggcgg
    43021 agcaacatat tctacaccag gaagcaaaga acgataagtg ttttgagagc ttccaatgtg
    43081 tttgatcgca tccagtattt ttccagtttc tcgattaact aagacgatcg tactatgacg
    43141 tcccattaat tcaacgatca atatgatatt ttgtagatca cccaactcgt ttcgtttagc
    43201 aaagtggaaa tggatcactc gatcattttc gatttgttcg atgtcttcta aaatagcgcc
    43261 atccaaatat tttcgaagca tcatcacgaa gttaggcgga ttatcaggat tctgataatc
    43321 gatttgggta atctgtacac gagcataact tgggtgcgca gataataata atcgatggtt
    43381 tttgccgcgt gaacgaatca ccagcacgat ttcattttca taaggctgat ggattttcga
    43441 gatccggcca gatagtaacg tttcacgcaa ttcgttgacc atcgcgtggg taaatacacc
    43501 atcaaaggac atggttcatt cctcatttct tttcataagt atattcatta taacgaaaaa
    43561 aagaagaaaa accaatttgg ccgaatagta aagcaaaagt ctagaaaaat cttggactaa
    43621 ttacataaaa aacagtgttt ctatcgttga aatccaaatt tttaggta
//
LOCUS       DAXLPI010000012        40424 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXLPI010000012 DAXLPI010000000
VERSION     DAXLPI010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.012"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            212..604
                     /locus_tag="ACF3T5_000547"
     CDS             212..604
                     /locus_tag="ACF3T5_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD3631181.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="ACF3T5_000548"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="ACF3T5_000548"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFD3631182.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2698..2943
                     /locus_tag="ACF3T5_000549"
     CDS             2698..2943
                     /locus_tag="ACF3T5_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631183.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3164..4774
                     /locus_tag="ACF3T5_000550"
     CDS             3164..4774
                     /locus_tag="ACF3T5_000550"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFD3631184.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4751..4963)
                     /locus_tag="ACF3T5_000551"
     CDS             complement(4751..4963)
                     /locus_tag="ACF3T5_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631185.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            5066..5935
                     /locus_tag="ACF3T5_000552"
     CDS             5066..5935
                     /locus_tag="ACF3T5_000552"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFD3631186.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="ACF3T5_000553"
     CDS             6100..7371
                     /locus_tag="ACF3T5_000553"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFD3631187.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="ACF3T5_000554"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="ACF3T5_000554"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFD3631188.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="ACF3T5_000555"
     CDS             8762..9025
                     /locus_tag="ACF3T5_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFD3631189.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="ACF3T5_000556"
     CDS             9470..9919
                     /locus_tag="ACF3T5_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3631190.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="ACF3T5_000557"
     CDS             9976..11394
                     /locus_tag="ACF3T5_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFD3631191.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="ACF3T5_000558"
     CDS             11394..12254
                     /locus_tag="ACF3T5_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFD3631192.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="ACF3T5_000559"
     CDS             12428..13201
                     /locus_tag="ACF3T5_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFD3631193.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="ACF3T5_000560"
     CDS             13348..13713
                     /locus_tag="ACF3T5_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFD3631194.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="ACF3T5_000561"
     CDS             complement(13861..15069)
                     /locus_tag="ACF3T5_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3631195.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="ACF3T5_000562"
     CDS             complement(15088..15366)
                     /locus_tag="ACF3T5_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631196.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15559..16215
                     /locus_tag="ACF3T5_000563"
     CDS             15559..16215
                     /locus_tag="ACF3T5_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFD3631197.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16657..17454)
                     /locus_tag="ACF3T5_000564"
     CDS             complement(16657..17454)
                     /locus_tag="ACF3T5_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFD3631198.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="ACF3T5_000565"
     CDS             complement(17451..18635)
                     /locus_tag="ACF3T5_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFD3631199.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="ACF3T5_000566"
     CDS             complement(18660..19907)
                     /locus_tag="ACF3T5_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFD3631200.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="ACF3T5_000567"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="ACF3T5_000567"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFD3631201.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="ACF3T5_000568"
     CDS             21887..22201
                     /locus_tag="ACF3T5_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFD3631202.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="ACF3T5_000569"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="ACF3T5_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFD3631203.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /gene="tmk"
                     /locus_tag="ACF3T5_000570"
     CDS             22886..23530
                     /gene="tmk"
                     /locus_tag="ACF3T5_000570"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFD3631204.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="ACF3T5_000571"
     CDS             23545..23874
                     /locus_tag="ACF3T5_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFD3631205.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="ACF3T5_000572"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="ACF3T5_000572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFD3631206.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25686
                     /locus_tag="ACF3T5_000573"
     CDS             24862..25686
                     /locus_tag="ACF3T5_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFD3631207.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25679..26026
                     /locus_tag="ACF3T5_000574"
     CDS             25679..26026
                     /locus_tag="ACF3T5_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFD3631208.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26095..26967
                     /gene="rsmI"
                     /locus_tag="ACF3T5_000575"
     CDS             26095..26967
                     /gene="rsmI"
                     /locus_tag="ACF3T5_000575"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFD3631209.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27076..28197
                     /gene="dinB"
                     /locus_tag="ACF3T5_000576"
     CDS             27076..28197
                     /gene="dinB"
                     /locus_tag="ACF3T5_000576"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFD3631210.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ACF3T5_000577"
     CDS             complement(28251..28853)
                     /gene="nrdG"
                     /locus_tag="ACF3T5_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFD3631211.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ACF3T5_000578"
     CDS             complement(29168..31357)
                     /gene="nrdD"
                     /locus_tag="ACF3T5_000578"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFD3631212.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31683..32450
                     /locus_tag="ACF3T5_000579"
     CDS             31683..32450
                     /locus_tag="ACF3T5_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631213.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32440..34482
                     /locus_tag="ACF3T5_000580"
     CDS             32440..34482
                     /locus_tag="ACF3T5_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631214.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34763..34912
                     /locus_tag="ACF3T5_000581"
     CDS             34763..34912
                     /locus_tag="ACF3T5_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFD3631215.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34923..36437
                     /gene="dltA"
                     /locus_tag="ACF3T5_000582"
     CDS             34923..36437
                     /gene="dltA"
                     /locus_tag="ACF3T5_000582"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFD3631216.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36434..37639
                     /gene="dltB"
                     /locus_tag="ACF3T5_000583"
     CDS             36434..37639
                     /gene="dltB"
                     /locus_tag="ACF3T5_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFD3631217.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37684..37917
                     /gene="dltC"
                     /locus_tag="ACF3T5_000584"
     CDS             37684..37917
                     /gene="dltC"
                     /locus_tag="ACF3T5_000584"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFD3631218.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37920..39188
                     /gene="dltD"
                     /locus_tag="ACF3T5_000585"
     CDS             37920..39188
                     /gene="dltD"
                     /locus_tag="ACF3T5_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFD3631219.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39329..40411
                     /locus_tag="ACF3T5_000586"
     CDS             39329..40411
                     /locus_tag="ACF3T5_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFD3631220.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
BASE COUNT        13015 a         7089 c         8463 g        11857 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgacttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaaacaaaaa agatgcttat gatgcattga aaatcgcaaa agaaaaagtg
    25141 cagttgcgtg agttggaaat gaaaatggtc aaagccgaat acacatttga tcgaaataaa
    25201 ttgatttttt ctttcacagc ggacgggcga attgacttta gagaattggt aaaagatctg
    25261 gccgctgtat ttcgtactcg tattgagctt cgacaaattg gtgtccgcga tgaagcaaag
    25321 ataataggcg gaattggtcc atgtgggcgt ccattatgct gttcttcttt tttaggcgat
    25381 ttcgtgcctg tatcaatcaa aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa
    25441 atttccggtt tatgcggacg cttgatgtgc tgtctgcaat atgagagcga agaatacgag
    25501 acagcgaaaa aagaactccc tgactttgga aaagtaattg ctacacctga tggtaaaggg
    25561 aaagtcgtag ggttgaatct gcttagccgt atcgttagta ttcggctgac tggacgggaa
    25621 aatcctgtcg aatatgaatg ggatgaattg aaagaattta tccaagtagg tgagatgaat
    25681 ggataagaga tcattatacg acggattgaa tcaattagaa tcagagttga gaaatacatt
    25741 aacagaactt gttgaaatga agcatgcttt gcatgagatc gtagaaaaaa atacaacttt
    25801 ggagatggaa aatcaacgtc tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc
    25861 tccagctgaa aatgtgaagc aggaactttc taagtctcgt atgaatcttg aaaaaattta
    25921 tgaagaagga ttccatgtct gctacgattt atatggatca agaagggaca atgacgagcc
    25981 ttgtgcgttt tgtctagaag tgatctatcg cgaaagcggt cgatgaccaa taacaagagg
    26041 agactggagc ggagaaatac tgctccagtc tttttattaa ggagattgtg acagatgtat
    26101 aatcaaaaaa gtttcaaggg aaatccggta tatggggatt tatatcttgt accgacgcca
    26161 attggaaatt tagaagatat gagctacaga agtgttcgta tgctgcaaga atccgatctt
    26221 atcgcaagtg aggatacgcg taatacacaa aagttattga atcattttga aatccataca
    26281 ccccaaaaaa gtttacatga acataattat aaagaacgtg tccctcaatt gattgctttg
    26341 ttaaaagaag gaaaaacgat tgctcaagtc agtgacgcag gaatgccttc catcagcgat
    26401 cctggacatg agctagtttt agcctgcata aaagaaggga tttctgtgat ctctatccca
    26461 ggacctacag ctgggatgac ggctttgatt gcttcagggc tagtgcctca accattttta
    26521 ttttatggct tccttccacg taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa
    26581 gaacagacag ttacgctgat tttttatgag tcaccttatc ggatcactgc tacattgacg
    26641 aatttcttag aaatttttgg aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc
    26701 catgaagaat accttagagg aagtacggaa gagctactcg cttatatcga agaacatcca
    26761 gtaaagggtg aatgttgtct gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa
    26821 gttgatgaag agttgggaac gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa
    26881 aaaccaaatg ctgctatcaa agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc
    26941 taccgtcagt accatgaatt agactgagat agtataagtt gtctataatg aaagactaat
    27001 gaggaaatca gctactaact tttcaaatct cttgaacgcg aacgaatgtt cttgtataat
    27061 gaataagagg tgaggatgat ggagctgcaa tttcctttac ataatgatac atcaagaaaa
    27121 atcattcata ttgatatgga tgcttttttc gcttcggttg aagaacgaga tcatcctgag
    27181 tttcgcggac atccattagt aattgccaga caccccagtg acactggtgg gcgtggagta
    27241 gtgactacag caaattatga agcaagaaaa ttcggaattc attcagcaat gagtgcacaa
    27301 aaagcgtatg agctttgtcc gcaagccatc tttaagcctg gcgaccatca taaatacagc
    27361 gatatttcca aacaagtacg ggaagtattc tatcgttata cagatattgt ggaaccagtt
    27421 agtatcgatg aagcgtatct tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc
    27481 aaaatcgccc gaatgatcca agcagatatt tgggagagtg tccagctaac atgttctgcg
    27541 ggagtaagtt acaataagtt tttggccaaa ttagcatcag actatcaaaa acctagagga
    27601 atcacggtag ttgcccctca agacgctgtg ccatttttaa aagctttgcc aattgaaaaa
    27661 tttcatgggg tagggaagaa aactgtacct agaatgcatg agctagggat ttatacagga
    27721 aaagacctat atgaatgcac agaaatgatg ttgattcgaa attttggaaa aatgggctat
    27781 tctctttatc gtaaagtcag aggaatccat gattctccag taaacgttac aagagagcga
    27841 aaatctgtgg gaaaagagca tacttatggg cagccgttgc aaacggaaga agcagtgctg
    27901 actcagttgc gccaactagc tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat
    27961 gggaaaacag tagttttgaa ggtcaggtac acggactatt ctactgtgac caaaagagtg
    28021 acattgccag aatatatcta taaaaaagaa gcactctttt atcaagcaag ccttatatgg
    28081 gaggaaatct tgggagtaga aaaaggaatc cgattgttag ggattacttt gacgaatctg
    28141 gacccgatga cttatgaaaa catcgtcctt ccattatggg aaaatcaaga aatataaaaa
    28201 cagctttgag agttgaaacc ggaaatccgt tttctctccc aaagctgttt ttatatcaag
    28261 tcttgctttt tgatttgttc ataggtttct gttgcatccg tacatttttc ccaaatcaca
    28321 acttgattgg attctaaaga tttttggacg tcaatgatcc gctggttgct acttccgcga
    28381 aattgtagtt ttaggtcacg tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt
    28441 tgcgaaagga gttctaattt atcttctgaa tcttgtagca attcatcaaa ggtatagcct
    28501 gtccatgacc agatatcttt tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc
    28561 agacaaacat ctgtattcaa aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa
    28621 tcgttgcgca agtcagcgat gatttgttct tctaattcag gtgtgaatgg tttgccatat
    28681 cggaaatttt gtgcagcttt gttgaagcag ccttcgcagg caaataagca gccactaacg
    28741 taaagactgt tgcggacacc ttcaccatcg acaaaattaa acggtttgta gtcagcaata
    28801 tagttttggc tgtagtcttt tgctaaccat tcttttggtt ttggatttct cataaaaaaa
    28861 cttctttcat ttaaggttca aagatatagc tattgaagat aaaatctact cgaccacata
    28921 aataagaaca aactgaccta aatccattgt tcaagaaagc ttgttcttat tttgatagag
    28981 ggcgttcaaa gatacaaagc catcgaaaga gggtggtgaa aaaagacttg tcatagcctc
    29041 ttattctgaa taaacggtgt tcaaaagctt gcatctgcag taataagccc aactaaacga
    29101 aaaatatgag gaactatttt tgtttagcta ttcttattac ttgatgcagg acagcttttt
    29161 cacaacctca tttcatgtgt ttcacacgtg aggaaatttc tttatggcgg ccatgtacca
    29221 tcggacgagc ttgcggattt cctaaatagc cacaagtacg tttgactaca tcacatgttt
    29281 ttggatcatg atttccacat tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac
    29341 cttcgaaacc acattgatag caatgatcga ttggcgtatt cgtaccaaga tacccgattt
    29401 tgtcatatgc aaaatcccat acagcttcta aagctttcgg attttgttgg agcatagggt
    29461 attcacagta gtggatgaat ccacctgaac aatattttgg ataatccttc tcaaattcca
    29521 atttttcaaa tggcgttggg tttttacgca catcatagtg gaaactgttt gtataatatt
    29581 ctttatctgt gatattttcg atgatgccga atttttctgt atccaaacgg cagaaacggt
    29641 cagttagact ttcacttgga gttgaataga cactgaaatg ataaccatat tcatcccccc
    29701 atgcatctgc attagctttt aattctttga cgatatcaag tgtgaaagct tttgcttctg
    29761 ggtttgtttc ccattcgcca ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc
    29821 ctaacgatac agtagctcga cggtttttga ataattcatt gacagaatcg gtacgtgaaa
    29881 gacgtttccc gaaagcacca tacatatata aaattggcgc atttgctgga atggcttctt
    29941 tacaacgttc tacacgatag agaagcgcat ctttcatgat ttctaagcgg tcagacagta
    30001 actgccagaa tttattctga tctccttttg attcgatcgc gattcttggc aaattcaatg
    30061 taacaacacc taaattcatt cgtccgacat ttacttcttg accattttcg tctttccacc
    30121 cttgcaagaa tgaacgacag cccataggta ctttgaaact tccagttaat tcaacgattt
    30181 tatcgtaatt cagaatatca ggatacattc ttttcgttgc acattccaat gctagttgtt
    30241 tgatgtcgta attaggatct tcagggttta agttcacacc ttttttcaaa gtgaagatca
    30301 gtttagggaa gatagctgtc cgcttttcac tccctaatcc attgattcga atctgtaaaa
    30361 ttgctttttg aatttctctt tcaaaccagt tttctcctag tccaaatcct aatgaagtga
    30421 aaggagtttg tccatttgaa gtaaataagg tattgatttc atattccaaa ctttgcatag
    30481 catcgaagat atcttttttg gtttttgctt ttgcatattc tttttggcgt tctggcgaat
    30541 cgatccattc ttccgcgtct tttagatgtt ttttatagtt caattctgca aaaggagcaa
    30601 gcaattcatc tgttcggtcg gcagaacagc caccatattg acttgacgct acattggcaa
    30661 taatctgtga gatttgtgct gtggcggtct ggatagattt tggcgattcg acttcggcat
    30721 tgccgatttt gaacccattg ttcagcatgc ctttgaaatc aatcaagcag caattcgtca
    30781 tcggtgtata aggatgataa tccaaatcat ggtaatggat atcgcctttt tgatgcgcat
    30841 ttgctatatg tggtggcaac attttcagtc cgattgattt tccaacgatc ccggctgtca
    30901 gatctcgctg tgtattgaaa acattactgt ctttatttgc gttttcgttc acgacagttt
    30961 ggtccttgtt gattagcttt tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa
    31021 aatctcgttt ggtacgataa ttgatgtact cttctgctaa ttcatattcg tttttttcta
    31081 gtaatatatg ttccacgatg ttttgaatct catagatttt gacattttca gcaaaccggc
    31141 ttttgatttc ttggacaacc aagtctgcaa tcatttgaat tttttcataa gtaagtggtg
    31201 ataatgagcc gtgaattttc ttttctgctt tgatcagtgc ttcattgatc ttttgttcat
    31261 cgaaatcaac atggcggcca tcgcgtttga tgactttcat ctgggatgtc tctggctgct
    31321 gagtaacgac agttttttct tcataattta ctttcattat cttcaactcc tgctaaattg
    31381 ttcttgtcta tcataaaaca aaatcgggga aggagcaact aaatatcggc tatatatagt
    31441 attcaatgtg ctgtataatt ggatttctat actatatgta gtgtatattt tattgtgaaa
    31501 aaaggtgaaa acttgtagct ggtggatttg ttttttgcat atttaatttt gctgaataaa
    31561 ataattgaat aaaatttttt attcagtcca agtaaaagtg acaaaaacat aagagcaaat
    31621 tattcgtaga tatgctacta tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag
    31681 aattggaaaa gatgatcgaa gtgaaacatc taagtaaaag ctacggcagc aaactttctc
    31741 ccgtcaaagc cttagataat atctcattca cagtagaaga aggcgagttt gttgggatta
    31801 tgggcccaag tggtgcagga aagacaacgc tgatgaatat cttgtccacg atcaatttgc
    31861 ctacgatggg cagtgtctcg atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc
    31921 tcagtgactt tcgtcgaaaa aaactaggat tcatttttca agagttcaat ttgatcgata
    31981 ccctaaatgc aaaagacaat atcctattgc ccctagcagt tgaacggatg actaaagagg
    32041 aaatggaaaa gagagtaaga catgtagctc aattgttgaa tatagaagaa ctgctcaaac
    32101 ggtatcccga tgaactttct gtcggacaga gacaacgaat tgcagctgct agagcacttg
    32161 ttgttcagcc acaagtgatt tttgcagatg aaccaactgg ctctttggac tcgaagtcgg
    32221 ctacagaatt gttgaactac ttgaagacca tgaatcaaaa agaaaaagca acgatactgt
    32281 tagtcaccca tgatccatat acagcaagct actgcgatag aattttattt attaaagatg
    32341 gtgtgatttt ttctgaagtt gtcagaagag ggacaagaag agagttcttt gagaaagtaa
    32401 tagacatgca ggcgacgatt ggcggaggag gaaaaatgaa tgctgtataa actagccgtt
    32461 aaaagttttt ttcggcagtc acgaggctat cttgtttatt ttttcagttt gactttatcc
    32521 gtaatgatct attattcctt cagcgcaatg acctacgatc aatctttagt cagaagagca
    32581 agtcaagata cttcgataga tggtggattg agttttggtg gatttatcat tactgttgtt
    32641 ttgctttttt tcgtattcag tgccaaccga tttttcttga atagaaggca aaaggaaata
    32701 ggcatctatc agctttttgg tatgaataaa ctgcagatat caggtatata tgtgttagaa
    32761 atcatgataa ttggattgtt cgcctgtatt tctgggatat tactcggtat tattttttcc
    32821 aaactctttt caatgatttt ggttcgaatg atggatatgg atcttactag tccatttttt
    32881 atctccattc catcagtagt agatacttta tttgcttttt tcatcatcct tttagcagta
    32941 tctgtatatt caatatggaa aatatggcgc tatccaatga ttcgaagttt tggagaaaaa
    33001 gaacaagcag atagttcaac aatgcgcttc aggacaaggc accgattact cgcagtcatc
    33061 gggttactac ttttaacgag cggatatttt ggagctagtc attttaggga aatcacttca
    33121 tggatgatca gttatcatta tcttgataca ttagttttct tttttccttt tttaattttt
    33181 ttcgtttgtg tggtcggtac ttatttgttt ttctgttata catttcggtt atttttgatc
    33241 atattgagta aatggaagat ttattactat ggtatcaatt ttttgataat gggtaacacg
    33301 caagttcatt tgctcaaaag ttggcgtaca aattctctga ttacagtcat tctcggaatt
    33361 tctttaatga caattggcgg cacggctagt atctctacta ttttatccta ccaggagaaa
    33421 gtttcctccc cgatggacta tcagtttgat gttgagacag agaaaaaaat caagccgatt
    33481 ctagcagagg aagggcagaa gataactaaa gaaatcacct tgcattataa agtcattggg
    33541 agtatctata gtcaaaatct cgaagaacct atgtttcaaa ctgctaatct gatcacggaa
    33601 acacaatatc aagccttcag aaaagttaat ccagatttgc cgaacatttc tttgaaatca
    33661 cctggtcata ctgttttgct tgatcaaatg caaactcttt ttcggaatat ttcattatat
    33721 ggatctagta ttggactgcc agataatcaa gaattgaaga ttcaacaaat gcttccaagt
    33781 gtattaggtg acgaaacaat ggtatatcta agtcctgtac tgattgtaga tgataccgtt
    33841 tttgaaaaag caaaaggtgt ggattatcaa gtagtcaatg tagatgtatc aggtggaaat
    33901 aatgaaaaaa tagatgaacg actgagcaat gacctgtcga taaagtggct caatgctgtt
    33961 tattattctt atgatatcgt aaatggaagt ctacaaggag aaattagcac aaagaaactt
    34021 ccggatgacc aaatgaagga agcacaattt agccaagaaa gctctcgttt gaatttctct
    34081 tccagctatt cgaaattgcg tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca
    34141 ctatttgtcg ggatgatcgt gattattgct actggtagta ttttgacagt ccggcaatta
    34201 gcagaagcag aggaagaaaa agaaaattat cacctattga ctaagctggg gattccacaa
    34261 agaaggatca gacggatgat cttcgagcaa aacgcactta ccttctttcc acccatgata
    34321 ttagggatca ctcacgcagt atttgcaatc aatgtcttca ctcagtatat aaaaactgcc
    34381 gattactggt tggcttatct tgtgagtggc ttattgatcc ttatctatct tttgctttat
    34441 gtcattacga gtcagttata ttgtcggatt atcgaggaat gatatacgga ataagacaag
    34501 ataataaata gaactaaatt ttgaatacca tttatttgga ataagaggtt gtgacacgat
    34561 ttttgtcaca acctctttat tttttatatt ttttaaaagt acgagaaatt ataaagaaaa
    34621 aagcagtgat ttgatgtgct atttttataa aagctatgtg acaaaactgt tagtaaaaaa
    34681 acggtttctg taagctagac acatgtttct ttttgtcaaa attagttaca ctttgtgtga
    34741 ggttatatga ggagggaatc caatgaacaa atggcgttct aatccaaaat tgaattattg
    34801 gggaaaattc atcattcgaa caatttttta tagtgcagta ctgctgttcc tgatttatct
    34861 gtatcactat aaaaatattc aaggaggaac ttttatctat aacgaatttt aggaggaaga
    34921 gtatggaaat caaaacgatt attgaagcaa tcgatgattg gggaataaat gaacctgatc
    34981 gagttgctta tcaggcggat gagacgcata cttttggtga attgaaacaa gcatcagacg
    35041 cacttgctta ttatttagaa aaaaaggtag aaggcgatgg accaattgtc gtttttggca
    35101 atttggagtt tgaaatgatt gtctcatttc taggggttgt caaatctggc catgcctatt
    35161 tgccgattga agagcataca cccaaggagc gtatcctatc gatctttcgg gtggctaaac
    35221 catctatggt catcagtatc ggggactgga tagaagatat tccttcggtt cctgtcataa
    35281 caaaaaaaga atttcaagaa atcaagaaga taccagtagg attttatccc agaaatagcg
    35341 taaaaggtac agaaaatttt tatattattt ttacttctgg tactactgga gaaccaaaag
    35401 gcgtacaaat cagtcatgat aatctagtca gctttgttca atggacgatg gaagactttg
    35461 ggatcaagcc tggtatgcat ttcttagccc aagcgccatt ttcttttgat ttatcggtat
    35521 tttctattta tcctgcatta gtttctggcg gaatgctgaa gccgttaaat aaagcagttg
    35581 ttcaagactt tcgtcaatta ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt
    35641 ctactccatc atttatggat atttgcttga tggaacctac attcaatgga gaaaatgtgc
    35701 cggaattgga catgttttta ttttgcggag aagaattgac gaaaaaaact gcagaaagtc
    35761 tacttgatcg attcccaaat gcacggatat ataatactta cggtccaaca gaagcaactg
    35821 tagctatttc aagtattcag attgaccagc atgtactaga tagttatgac cgactaccga
    35881 ttggctacgt aaaaaaagac acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa
    35941 aaggagaaac gggtgaaatc attattgcag gaccaagcgt atcaaaaggt tacctgaaca
    36001 atcccgaaaa gacagctgct gcgttctttt gtcaaaatga cttagcaagt taccatacag
    36061 gggatgcagg tagactagat gaatatgggc ttttatttta tgaaggacgt atggatcagc
    36121 aggtcaaatt acatggatac cggattgaac tcggggatat cgaacattat ctgctccaag
    36181 acaaccgaat caaacaagct gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc
    36241 tggttgcttt tgtcgtatta gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat
    36301 ctatcaaaga acaattacta gaagttgtaa tggattatat gattcctcag aagttccatt
    36361 atgtcgatct gctgcctcag acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg
    36421 aggtgaatcc tgaatgatgt tacctcatat gatcccttat gcagagccgt tttatttttt
    36481 tcttttagcc attgcgcttg taccgatcgt tctatcttta ttgatatggg aaaaaagatt
    36541 accagtttat caatcagcag tgacactttt ctttttattt gtcagctttg gaggtaatta
    36601 ttggcaacag ggagcggcgc tgattgggta tgttttgtgg cagacgatac ttgtatgggg
    36661 ctattttgtt tatcggcaaa ggaaaaatca gacaggatgg ttttttgccg ccgttatttt
    36721 agcgataatt ccactgtttt taacgaaagt gactcctttt tttgaacatg gaaaagcatc
    36781 gttacttggt tttttaggaa tctcttattt aacctttaaa tctgtacaaa tgatcatgga
    36841 aataagagat ggcatgatca aaacatataa gccatttcgt tatatccagt ttctgctgtt
    36901 ctttccaaca atctcttctg gaccaatcga tcgctatagg agatttgaaa aagatcagct
    36961 ccatccgcct gttcgtgata aatatgttga tcttttggga aaaggcgtcc aaaatatttt
    37021 tgtcggtttt ttatataaat tcattatcgg ttattatttt ggtcaagtac tgcttcctgt
    37081 agtgggacgc attgccttac agcatggtgg tatttcatgg gcattagtag gttatatgta
    37141 cgtgtacagt atgtatttgt tttttgattt tgctggctat agcttatttg cagtcggtac
    37201 aagctacatg atgggatatc agacaccaat caatttcaat aaaccttttt tatcatggaa
    37261 tatcaaagaa ttctggaatc gttggcatat gacgctgtcc ttttggttcc gtgattatgt
    37321 atatatgcga ttgatgttct tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat
    37381 gtcaaatatc ggttattttg ctttgtttct tctaatgggt atatggcatg gattgacgtg
    37441 gtactacatc gtatatggac tgtatcatgc ttgtctgatc tgtctgacag atgcatggat
    37501 ccgatttaag aagaagcata aagagcagtt gccttctaac aaatttacgc atggcttagc
    37561 cgtatttttg acatttcaag cagtatgttt tagcttcctg attttttcag gatttttaga
    37621 taaacttttt tttagctaac gaatagtgac atattaattt atataacaaa ggagaaaagg
    37681 aaaatggaag aacaaatttt atcaatctta gaagaaataa caggaacaga tgaagtaaga
    37741 aaaaatcgtg atattgatct atttgatgaa gggttgcttg attctttagc atctgttcaa
    37801 ttattagtcg agctagacgg agagctggat atccaagtcc ctgtatctga attcgagcga
    37861 gaggattgga gcacaccgaa taaaatcatt caacaagcaa ctgcattgaa gggttgagta
    37921 tgaaaaagaa actttttgct attttcggcc cggttgtttt agctgttggc ttactgtttg
    37981 tgttttttac ctcctcctat caagtagata tctctaatcc agaaacgatt agacgtgcct
    38041 cttcttccat gtcagtgaac gtgttgaaag gagatgcgat aaaaaattat gcattatctg
    38101 aaaaacaata tattcctttt tttggctcaa gtgaattaag cagaatcagt ccatttcatc
    38161 cgtctgttct ggcagaaaaa taccagcgaa actatcgtcc atttttgctg ggagcacccg
    38221 ggacacaatc gttaagccaa tatatgatga tgcgttctgc aggagatgct atgaaaaaca
    38281 agaaagtcgt ctttattatt tctcctcagt ggttcgttaa aaatggtgta aaaactgatt
    38341 actttaatac gtattattct gaacttcaaa catacgactg gttgttttca atgaagaaag
    38401 taacgcctgc tgatcgctat ttagctagaa gattattaac tttctcaaaa gtaaaagaga
    38461 atgatacgtt gacggcgatt ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat
    38521 ctttgaatca gctgcgcagc caatggaaca tgctgaagag agaggatgaa gtattcagca
    38581 atattgggtt gaaagatcga cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac
    38641 aataccaaga aacagaatta tccattttag caaatcaaat tggagaacga gaaacgacga
    38701 ataatccatt cgggttgaaa aacgattttt atacgcatcg tatccgagcc cacgagccag
    38761 agctcaaaca gtcacaaaaa aattgggatt atcgtttttc gccagaattt tctgattttc
    38821 agctggtctt ggatcaatta gcaaaaaacc ataacgaagt attgttcatc atcccaccgg
    38881 ttaacgagaa atggagcgat tataccggac tatctcaaga aatgttacaa ggatttgcca
    38941 aaaagatcaa atttcaatta aatagccaag gattcaatcg aatagctgat tttgtcaatc
    39001 aagcgggtac caactatttt atggaagaca cgatccatct aggctggaaa ggctggttgg
    39061 ctgcagatca acagatcaga ccgtttttag aagaaaatca tattactgct agtaagtatc
    39121 atcttgacga tgcttttttt agcaaatctt ggcaacatca aataccagat aaactacaat
    39181 taaaataaag aaaacaggcg agtgttgctc gcttgttttc tttatttttg aatattctga
    39241 ataaaagata aaaataccaa gtttgcctga acaaaaatca taagcaatgg tataatcaca
    39301 atatgaatat agaaatgggg gaaaacattt ggatacaaaa gaagaacagc tacttattga
    39361 tttaacttct gcaaaaggag tacctggaaa tgaagaagag gtaagagaag tattcagaga
    39421 atatgccaaa ccattcgctg atgatatttt ttatgacggt ttaggcagtg tgattgccaa
    39481 acatggtgct ggcggaggcc ccaaggtctt tatttctgga catatggacg aagttggttt
    39541 tatggtaaca aaaataaccg aaaaaggatt cttggaattc caaactcttg gaggttggtg
    39601 ggggcaagtc atgcttgctc agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca
    39661 cggtgtgatt ggttccaaac caccacatgt tttaacacct caagtaagaa ataaacctta
    39721 tgaaataaag gatatgttca ttgatattgg cgcatcaagc caagaagaag ccaaagagtg
    39781 gggcatacgt cctggagata tggtcactcc atatattgaa tacaaacgaa tgaatggcag
    39841 taaatattta ctggcaaaag catgggataa tcgtatcggg acagctgttt ctctgcgagt
    39901 attagagaat ctatctaaag aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca
    39961 agaagaagta ggcttgcgag gtgctcgaac aagtacacac cttgtaaatc ccgatattgc
    40021 ttttgcttta gatactggta cagcaggaga cactccagga atgacaccaa aagaagcaga
    40081 ttcgatttta ggaaaaggcc cacagatttt gatttttgat gcatctatga tcccgcataa
    40141 aaaattattg aattttgtta ttgatgtagc agaggaaatg gatattccat tccaatatac
    40201 agtgatttct ggcggaggaa ctgatgcagg acaaatgcat ttgacaagag atggtgttcc
    40261 gtctgtagcc attaccgttc ctgttcgtta tctgcattcc catacatcag tgattcatga
    40321 agatgattat ttgaatacag tgaaactagt cacagaatta gtgaaacggc ttgatagtga
    40381 aacagttgaa aaaattcgga gttatgaata atgaataata gaat
//
LOCUS       DAXLPI010000013        39588 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXLPI010000013 DAXLPI010000000
VERSION     DAXLPI010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.013"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(132..1181)
                     /gene="recA"
                     /locus_tag="ACF3T5_000587"
     CDS             complement(132..1181)
                     /gene="recA"
                     /locus_tag="ACF3T5_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFD3631221.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(1290..2531)
                     /locus_tag="ACF3T5_000588"
     CDS             complement(1290..2531)
                     /locus_tag="ACF3T5_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFD3631222.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(2771..3349)
                     /gene="pgsA"
                     /locus_tag="ACF3T5_000589"
     CDS             complement(2771..3349)
                     /gene="pgsA"
                     /locus_tag="ACF3T5_000589"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFD3631223.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(3558..4481)
                     /locus_tag="ACF3T5_000590"
     CDS             complement(3558..4481)
                     /locus_tag="ACF3T5_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFD3631224.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(5220..6506)
                     /locus_tag="ACF3T5_000591"
     CDS             complement(5220..6506)
                     /locus_tag="ACF3T5_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFD3631225.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(6499..7767)
                     /locus_tag="ACF3T5_000592"
     CDS             complement(6499..7767)
                     /locus_tag="ACF3T5_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFD3631226.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            8019..8480
                     /gene="mscL"
                     /locus_tag="ACF3T5_000593"
     CDS             8019..8480
                     /gene="mscL"
                     /locus_tag="ACF3T5_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFD3631227.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(8539..8985)
                     /locus_tag="ACF3T5_000594"
     CDS             complement(8539..8985)
                     /locus_tag="ACF3T5_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3631228.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(9078..9323)
                     /locus_tag="ACF3T5_000595"
     CDS             complement(9078..9323)
                     /locus_tag="ACF3T5_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFD3631229.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(9438..10016)
                     /locus_tag="ACF3T5_000596"
     CDS             complement(9438..10016)
                     /locus_tag="ACF3T5_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFD3631230.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(10093..10623)
                     /locus_tag="ACF3T5_000597"
     CDS             complement(10093..10623)
                     /locus_tag="ACF3T5_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFD3631231.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(10627..11487)
                     /gene="rlmB"
                     /locus_tag="ACF3T5_000598"
     CDS             complement(10627..11487)
                     /gene="rlmB"
                     /locus_tag="ACF3T5_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFD3631232.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(11480..11875)
                     /locus_tag="ACF3T5_000599"
     CDS             complement(11480..11875)
                     /locus_tag="ACF3T5_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFD3631233.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(11872..13284)
                     /gene="cysS"
                     /locus_tag="ACF3T5_000600"
     CDS             complement(11872..13284)
                     /gene="cysS"
                     /locus_tag="ACF3T5_000600"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFD3631234.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(13281..13817)
                     /gene="epsC"
                     /locus_tag="ACF3T5_000601"
     CDS             complement(13281..13817)
                     /gene="epsC"
                     /locus_tag="ACF3T5_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFD3631235.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(13903..14142)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14139..15599)
                     /gene="gltX"
                     /locus_tag="ACF3T5_000602"
     CDS             complement(14139..15599)
                     /gene="gltX"
                     /locus_tag="ACF3T5_000602"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFD3631236.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(15769..16926)
                     /locus_tag="ACF3T5_000603"
     CDS             complement(15769..16926)
                     /locus_tag="ACF3T5_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFD3631237.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(16960..18330)
                     /gene="radA"
                     /locus_tag="ACF3T5_000604"
     CDS             complement(16960..18330)
                     /gene="radA"
                     /locus_tag="ACF3T5_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFD3631238.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(18393..18920)
                     /locus_tag="ACF3T5_000605"
     CDS             complement(18393..18920)
                     /locus_tag="ACF3T5_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFD3631239.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            19150..19956
                     /gene="proB"
                     /locus_tag="ACF3T5_000606"
     CDS             19150..19956
                     /gene="proB"
                     /locus_tag="ACF3T5_000606"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFD3631240.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            19953..21200
                     /locus_tag="ACF3T5_000607"
     CDS             19953..21200
                     /locus_tag="ACF3T5_000607"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFD3631241.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            21276..21620
                     /locus_tag="ACF3T5_000608"
     CDS             21276..21620
                     /locus_tag="ACF3T5_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631242.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(21737..22063)
                     /locus_tag="ACF3T5_000609"
     CDS             complement(21737..22063)
                     /locus_tag="ACF3T5_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631243.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(22678..23880)
                     /locus_tag="ACF3T5_000610"
     CDS             complement(22678..23880)
                     /locus_tag="ACF3T5_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3631244.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            23997..24512
                     /locus_tag="ACF3T5_000611"
     CDS             23997..24512
                     /locus_tag="ACF3T5_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFD3631245.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(24711..25157)
                     /locus_tag="ACF3T5_000612"
     CDS             complement(24711..25157)
                     /locus_tag="ACF3T5_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFD3631246.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(25204..26682)
                     /gene="msr(C)"
                     /locus_tag="ACF3T5_000613"
     CDS             complement(25204..26682)
                     /gene="msr(C)"
                     /locus_tag="ACF3T5_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFD3631247.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(27036..27665)
                     /locus_tag="ACF3T5_000614"
     CDS             complement(27036..27665)
                     /locus_tag="ACF3T5_000614"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFD3631248.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(27649..28113)
                     /locus_tag="ACF3T5_000615"
     CDS             complement(27649..28113)
                     /locus_tag="ACF3T5_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFD3631249.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(28600..29868)
                     /locus_tag="ACF3T5_000616"
     CDS             complement(28600..29868)
                     /locus_tag="ACF3T5_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFD3631250.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(30099..30836)
                     /locus_tag="ACF3T5_000617"
     CDS             complement(30099..30836)
                     /locus_tag="ACF3T5_000617"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD3631251.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(30903..31472)
                     /locus_tag="ACF3T5_000618"
     CDS             complement(30903..31472)
                     /locus_tag="ACF3T5_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFD3631252.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            31600..32472
                     /locus_tag="ACF3T5_000619"
     CDS             31600..32472
                     /locus_tag="ACF3T5_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD3631253.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            32814..33686
                     /locus_tag="ACF3T5_000620"
     CDS             32814..33686
                     /locus_tag="ACF3T5_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFD3631254.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            33683..35641
                     /gene="nagE"
                     /locus_tag="ACF3T5_000621"
     CDS             33683..35641
                     /gene="nagE"
                     /locus_tag="ACF3T5_000621"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFD3631255.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(35973..36824)
                     /locus_tag="ACF3T5_000622"
     CDS             complement(35973..36824)
                     /locus_tag="ACF3T5_000622"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD3631256.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            36947..37414
                     /locus_tag="ACF3T5_000623"
     CDS             36947..37414
                     /locus_tag="ACF3T5_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFD3631257.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(37677..38159)
                     /locus_tag="ACF3T5_000624"
     CDS             complement(37677..38159)
                     /locus_tag="ACF3T5_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFD3631258.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(38259..39122)
                     /locus_tag="ACF3T5_000625"
     CDS             complement(38259..39122)
                     /locus_tag="ACF3T5_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFD3631259.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        11229 a         8421 c         7119 g        12819 t
ORIGIN      
        1 gaacagatat ttttatttta tgtacccact cgtataatta aataactcac gatttcttta
       61 ttcaataaaa aaacggcagg tcaaggagag tacctactct tcagaccagc cgtttttgtt
      121 tatcataatt tttattcttc tactaaaggc aattcttctt gtcctttttc atcttctggg
      181 acagcgactt cttcaccaat accataagct gctctaacta gagcagaaac ttctgccatc
      241 atttcaggat gattagccat atagatttta gcgttttccc gtccttgacc aatccgatct
      301 tctttataag aataccaagc accgctttta tcaacaatgt ctttttctac tgccatgtct
      361 agaagttctc cttcttgtga gatccctaaa ccatacatga catctacttc agcgattttg
      421 aaaggaggtg ctactttgtt ttttacgact ttgatctttg tacggttacc aacaatatct
      481 gtaccttgtt tcaattgttc tgcacgacgt acttccaaac ggatcgttgc gtagaatttc
      541 aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca tcacgcctac tttttcacgg
      601 atttggttga tgaaaatagc gattgttttt gttttgttga tcgaaccaga gagtttacgc
      661 aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg agtcacccat ttcgccgtcg
      721 atttctgctc gtggaactaa agcagcaact gaatcgacta ctacgatatc tacagcccca
      781 cttgatacta aagcatcagc gatctctaga ccttgttctc ctgtgtcagg ctgtgaaaga
      841 agtagttcat cgatattcac acctaatttt tgtgcatatt gcggatctaa cgcatgctca
      901 gcatcaatga aagcggccgt tccgccattt ttttgtactt ctgcaatagc gtgtagtgca
      961 actgttgttt tacctgaact ttcaggtcca tatacttcga tgatacgtcc acgggggtaa
     1021 ccgccgacac ctaaagcgac atctaacgca agagagccac ttggaattgt agagatttgt
     1081 tggtcgattt tttcgcccaa tttcataata gagcctttac catagctctt ttcaattttt
     1141 ttcagtgcag catctaaggc tgctttacga tcatctgcca atacgataat cctccttata
     1201 tttacaaatc agagcaacat ttcattatcc ttatcatatc ttttttagtg aaaaaaagca
     1261 agaaaaaacc gaacaaatat tcgcatttat cattttctta aaagctgttt tctcaaaaga
     1321 tcaaatcctt gcataaccgc attatatctg atagcagcac gatctctggc aaagtgatat
     1381 ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc atactgttcc tgcagattga
     1441 ccttctaacg tgtccggtcc agccactccg gtgaaagcta cactgtaatc tgttcttgct
     1501 atctcacgtg cttgaatggc catctgctcc acacattctt tgctgactgt tccatattct
     1561 gctaatagct ccggatcaat ctccagaaaa ccagcttttg tttggagcga gtaggtaacg
     1621 aatccgcctg gaaatacttc agatacacca gcaatgtcgc ctaatgcggc ttggaaggcg
     1681 ccggctgtca aactttccgc tgctgttaca gtctgcttgt tttctttcag tagttccact
     1741 acgacttttg ctagcgaatt atcatcgccg tatccataaa aatattcccc tactcgttct
     1801 tggatttttt cttctaatgc taaaagagct tgattccctg cctgaacatc atttgttttc
     1861 actgtcagtc gtaatgtcac ttcatttggt ttggcatatg gtgcgatcgt tggattgatc
     1921 tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg attcaccaat cccataaaag
     1981 cgcaaaactt tagaaattag tttttcttca gaaggaaaac gttcctctag taaaggacgt
     2041 acttgttcca caaacattgg tttcaactca ttaggcggac cgggaagaag gatatacgca
     2101 tgtgtatctg tttgataaaa agtccctaaa gcaagccctg tacgatttgg tagaggaacc
     2161 ccgccttcga taatctggga ctgttgcaga ttgtttttgg tcatttttcg atgggttgtt
     2221 tcaaaatatg ccaataattt tttgtatcct tccgtatttt gtatgagaga cttaccgagg
     2281 tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg gtcccaatcc gccacataag
     2341 agtatgagtt cacttcttgt ttcagctaga gcaatcaact cttctaaacg ctgctgatta
     2401 tctcctacaa ctgtttgata ataaacttcg atacctagat cagctagctg ttctgacaag
     2461 aaagtggcat tcgtattcac gacttgtcct agtaaaagtt ctgttccgac agctattatt
     2521 tctgctttca ttacttcccg ctcccttata tagtatacgc tgtattttta tattttttta
     2581 tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca cagctccttg ttccaaataa
     2641 acagtgttct cctgccaaaa cctcggtcat aagtcacaat atacataaaa catgaaaagc
     2701 tgttttttga aattatccct tgtgactcag agttagacgg caggctcaca acctctccac
     2761 taaacgatac ttacattgat cctttgaaca caccgctgtt tttgatgaag taatccacac
     2821 ctgaatagat agtaaagatc agacaagcat aaagcatgat ttgatctact ggaataccga
     2881 tcaggctgaa tgggatatta ttgataaaca gcaagataat agcaaccatt tgtgtagccg
     2941 tcttgacttt tcccggccaa gctgcagcca tgacttctcc atgttcgaca agtagtaaac
     3001 gcaagcctgt gacggctaat tcacgacaga caatgatcgc tactacccaa ccaggggcct
     3061 ttccttggtc aaccaacatg ataaaagcag tcataactag cattttatct gccaacgggt
     3121 ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg ggcaattttt ccatctaacc
     3181 agtctgtgat actagctaaa gcaaaaataa ctgcacctac tagctgtgtg acagccagtg
     3241 tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa acctacaacc aaaatgaaaa
     3301 tcgggatcat acaaattcta agtacagtta atttatttgg taaattcaca ctatcgactc
     3361 ctttcttccc tattctacgt gactatcagc gaagtttcca ccgatattat ttacttttcc
     3421 aaatgaattt attcaaagga gtatttttat tgaaattgat ctttttgaat taacgaagaa
     3481 ggaccgcgac acagatttgt cacgatcctt cttttttata cctacaagta aacagatgcg
     3541 taaactaatt tatacgatca ttctgcgtat tgaagagtaa ggttcacatt ttttggactc
     3601 tgattgtttt cccctggatt aactggtacg gattctccat tcgctttgat cgttgcattg
     3661 cttgctgcac caatcgtgat ggtcgcattt gttgctgttt ccggtaacac ggtactctgt
     3721 gtttcatttg ctgccagtgt cccttgataa acataggcat tgtctactag cacacctaac
     3781 catacacgat tgacagcagt aaattcaagg gttacaggct tggtagcgtc tgtgatattt
     3841 actgcgatgg cactgccaga atcttctcct gtagctattg ccattttctt ttcttcactt
     3901 gattttgttg ttgattctac tgtagaagac tctttggtct gcgtgctttg ctctgaggaa
     3961 gaggactcct cgactgatcc ttctacttta agcgaagaag ctgtgtctcc gattattgga
     4021 tcagcttgac gatcttgcca agtcatatag cccacgacaa cgacaatcgc caaagcaacc
     4081 aatcctaata aaatgacagg aagacttgtc caaaagcgac ttggatgcga ttcttcctga
     4141 tgcattgctt ttctagaagt acttactgtt tcaggctgcg gacgttcagg gaccgatcct
     4201 gtcaatttgc ttttcccatc aaaaacatcg actaaatggt ctccatcttc tcctacagct
     4261 tcagcgtatt gccgaataaa tgcccgcacg taaaatttac caggcaattg gtcaaaatca
     4321 tcattgtcga ttgcttccaa ataacgtttt tgaatttttg taatctgctg taattcatct
     4381 aaagatattt ttttattcag gcgtgcttgc cgtaattttt ctccgatggt ttcaaaagcc
     4441 actcgttcat cttctccagt ctgtttgatt ctccgaagca tctgctgttg caacgaaaga
     4501 cgttatgttt gggtgctgtg acaaaagtca tgtcacagct ccttttttcc gaataaacat
     4561 tgttctcctg ccaactcctc ggtcacaagt cacaatatcc aataaatata agaagctgtt
     4621 ttttgaactt gttccttgtg actcggagtt aaacggcagg ctcacgacct ctttcttaaa
     4681 cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac cggcaaaata taaggagata
     4741 tttttccgta tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttcctta
     4801 aacggtgttc aaaagcttgc atctgcggta ataagtccaa ccaagttaaa aacattaaga
     4861 agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt
     4921 ggtacacggt gttccagaag tagcttcttc ggcaataagc cgaaatatca caaaaatttg
     4981 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gaccacttct gtcacaacct
     5041 ctttgattac ttcgtttgat tttattttaa tataagttga ttaaaagaga gcatgatttg
     5101 ttttattttc tcactaattt ttccaacaca actaagaata ccataaaagc tgattttgga
     5161 ggaggttcct actacaagtc taaaacgaaa tttaagactt ttcctcataa aatactggtt
     5221 tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac ttataaatgc ttccccagct
     5281 gccagtacgt ctttcatttg gatcgaatcg atgatttcag gaagatcaaa taaagttcga
     5341 tcgccaaata aactttgcgt aaattgattg gcaatatatt cgatcgaatt taatgattgg
     5401 aaatattgtc caagcatttt cttcttcagc aaatcgagat ttgtttctga cacttccgga
     5461 tcatcagcga atccaagaat gatttctttg actttttctg ctgctttctc tggttcgtcc
     5521 gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca agctgaattc aaaaccaaag
     5581 ctgtcatcga taattccttg attgtacatc gctagatagt ttcgagaagt attgcctaaa
     5641 atcatttgga acaacagatt gattgctgtt ttatagcgta acagttctgt tccttcttgc
     5701 ggcaatgtgt ctaatccttt aataccaagt acaaatttat ctcgtgtaat ggctgcttcc
     5761 aatgcacttt gtttgatgat ttcttttgtg ttttcaggga aataacgtac aatttcttgc
     5821 tttggaggga aattttttgc ttcttggttt tcccgaataa gtttcatcaa tttctcaggc
     5881 tccatcttac caactacaaa taaaaccata ttgcttggct gataaaacgt ccgatagcac
     5941 gtatacaagt cttgagctgt gattttgtcg atactttcaa ccgtgcccgc aatatcgata
     6001 tgcaatgggt gtgtaggata caagttattt agtatgccaa aaaacatccg ccagttaggg
     6061 tcgtcttcat acatttggat ttcctgaccg atgattcctt tttccttgtt tacagtttct
     6121 tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg tcagattctt ttctacctga
     6181 tccgtcgtag aaaacagata gctggttttc gtaaaacttg tgaaagcatt ggcggaagct
     6241 ccttgtttcc caaataattg aaagacatca ccatcttctt tttcaaacaa cttatgttct
     6301 aaaaaatggg caattccatc tggaactttc accttttctt tttcaccata gggaataaat
     6361 tcgttatcga tcgaaccata attcgtactg aacaaaccat atgtcttgtg atagtcattt
     6421 ttaggcaaca aatacacggt caaaccgttt ggcagtactt catgataaag tgtttcattg
     6481 atttgctcat attctgtttt attcatttgc atttcctcct gctaaaaaga agattgcctg
     6541 caattcgatt tgctcagcta cttgttgtat ctctttgatc gttacgctct ccatacgttt
     6601 caaccattct tcatcagata attttgtttc cggcagccag ctgtccaaaa aagaagcttc
     6661 gattgccgct tgcggagagt ctagagaaag cagatattgg tttctcagca ttgcttttgt
     6721 ctgagacagt tccaactctg ttatttcacc atttcgcaaa ctttctaatt gctcgtgaat
     6781 cagatgaagt acttgatttc ggttcttttc atcgataccg gtttggacac tcatgaatcc
     6841 tcggaacgta tccacgctac tagaggcata ataagctaga ctttctttct ctctgacatt
     6901 catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg ttaaagacca ttagcgcaaa
     6961 acgttctggt tcatcgtaat acacatttgt ctgataagct aggttcagct ttgcttgaac
     7021 gattggctca cgtacttgtc gttccttcac gatattcact tgaggttggg tataaaacag
     7081 atctggataa actgtttccc gcgactcaaa tggtaattgc ccaactgctt cagctgcttt
     7141 gtccggatca acatcgccga caacaaaaat gtcgatttga tcatttgcca tcatttcttg
     7201 atagtaggac gctagcgaac tagcagtgat cttgtccaaa tcttcgacta cgcccaaccc
     7261 tgggatcttt tgttccgggc tgttttcaaa ataaagttct tgcaaggcat aggaagcaaa
     7321 agtttgttta tcttccttca aactttccaa atatagacgc agattgttct tttccaagtc
     7381 aaacgtcaac tgatcaaact gatgattttt aatatttgga taaaataaaa cctcttttaa
     7441 aaaatctacc gcttgagaaa aaaggtttgg atcattgaca tattcaccat tcacaaaact
     7501 gatccctgcg tttagccaat gtaaatttcc tttttttcta actgataatc caaagcttgc
     7561 gccataaagc tctgcaagtt gcgcacttaa ctttgtctga tccgaataat tcaaactatt
     7621 tgtttccaac atgctgctga gaagtgaacg tttcgtgatg acttcttgtt gcagacgcgc
     7681 agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt gttggtaaga catgaagatt
     7741 cacgccttct gccaattgat acaccatatt tatatgctcc ttttcacagt tgattgcccg
     7801 cttactcttt taatgggtca acctgccttt tttacttatt cctctgcttg aaatttataa
     7861 gttcaggcaa ttcgccgttt tttctaaatg atgttacact ttatagtata gcatagatgt
     7921 tttttatcaa aagaaacaga cggctaaaag cgtatattgt ttttttattt tttatttttc
     7981 gatatactaa agacaaataa aagaagggag ggtcattcat gatcaaagaa ttcaaagagt
     8041 tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt tgttattggt agtgcgttta
     8101 caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc actaataggt tggtttgtcg
     8161 caatgatcac aggtacttct gatttagaag gagcactttc aattcttgac tggtcgccga
     8221 caaaaggcgt cacatttgcg tttggtaacg ttattagtgc tatcattaca tttctgatca
     8281 ctggttttgt cttattctta gttgtcaaag cggcaaaccg tgcgaagaat ttacgctcca
     8341 aagaagagca agtggaagtg gaagaaactc ctactgcaga agattatctt agtgacatac
     8401 gcgatcttct tgccaaacaa gtggcagaag acaatacaca tccagtagag caaacaaatc
     8461 tttccgatac ttctaaataa taaagaatct aaaaaaaaga aagccgattt tcagtgtcgg
     8521 ctttcttttt ttagattctt aacttgctac ttgttccttt ttcatgtcga tatgtggaat
     8581 atcatcttcc aaatacacat ctgagattgc cttgaatcca aacgactcat aaaaagcttg
     8641 taggtattct tgtgcgccga tcacgattgg tttttctgga aaatttttct ggatcatgtt
     8701 aatcgtttct tgaacaatct ttcttcctaa accatcttta cggttgtcct gatgaacaag
     8761 gacacgtcca aaatggatac tctccgcttc ttcatatact cgcgtgtagg caaggatctt
     8821 cccatcagta tcttgaaaaa acgtatgcca agcttctcca tcaatgtcat caacttcttg
     8881 ataaggacac tgttgttcca caacaaaaac tgcgattcgt tctttgacga tcgcgtaaaa
     8941 ctctgctgtt gacaattctt caaatttctt tacctgatat tccactaagc aaacgccccc
     9001 tgctttttta ttcttagcat accgattcgc acacaaaaaa gcaaacgaac cttacgaatc
     9061 tcaggctcgt ttgcttttta aactgcttca gttaaaaatt cgatctcaac tgtccgagta
     9121 agtacatctg aatagctgta agatactcgt tcaaaagaat tttcttcagg atccaaatca
     9181 acaacgaata ctgaaggata ggtttctgtt aaaacgccgc ggcgttctgt ttgacgcttt
     9241 cttcctgtct gagcaaccaa catgattggg ctgccgatac gaccttctaa atctttcttg
     9301 attgaagcta aagttgttgg catacagttc acctctacta tcaactatac cacatatttt
     9361 taaaattttc aagtttacca taaaaaaaaa agaaatgcaa taaaaataaa taagaagctc
     9421 attaagattt ttctaaatta ttcctcatta taaaatttca agtgtttttt tattttgagt
     9481 tttactctcc cgtaagcgct cattacttgc ttgtgatcca ttgctaaaca ttcagccatg
     9541 tctacaaaat caacgccttg catataataa tgaaaaattt tagcttctat ttttgaaaac
     9601 tggatttcgc aattttcgat tgcttctttt aataaaagat gctgaattgc taaaggttct
     9661 tgcaatgtga tctctgctaa aaagtctggt ccttctgtct ctattttttg gtcgattgag
     9721 acttcatctt tttgatcacg acgcttcagc gcgttctgct tcctcaacag agaatagatt
     9781 tgattgctta taacgctttt ataataactt ccaaagctaa cacctttgct tttttcgtaa
     9841 gttctcaatg cctgatataa agcgatcctt gcttcttgtt cccaatcctc agaaacataa
     9901 tttcttaaat aataggtgtt tttcaaacgt agtaaaagtg gaaaatattt ttgatacagg
     9961 gcagcaaatg cttcttcgtc accattacgt gcagcgcgca ataactcctc agacataaaa
    10021 taattcctcc ataaattcgt ttatggagga atcttatcat caatgattca tcgaactgat
    10081 tagaacgata tgttatttat tttttctcat taaatcgtcc aatttttggg acaatttttt
    10141 taactgctct tcattccatg gagaattacg tcggaaatca ataaaatgca tatctttatt
    10201 ttcttgggct attgtttttt ccgttttttt tatcgtctta tataattcat aagcagaagt
    10261 tcgtaatgct ccttgggaaa agatcatcca ctgttctgct aaatcgcttg tcgccactgt
    10321 cacttgtgtc aaacggtcat tcagctgacc agcgattctc tcgatataac tgtcagccgt
    10381 ttcgtcttct ttcgtaaaaa caacatccaa ctggtatttc ttataatttt gttggatgcc
    10441 cggaactagt tgggcatcga agacaacgat gacttccatt ccttcatatt tcgcataatt
    10501 tgataaacga tgtaaaagag cttctcttgc atccgccatt ttgtcctgct ttttaagctg
    10561 gacaagttcc ggccacgaac caatcatgtt gtagccgtct acaatcaaca gctgtttttt
    10621 catggtctac tccccctgac gttgggaaaa agcctgatac ataagcaaac cagcagcaac
    10681 actggcattt aagctctgga cgtgcccggt catcgggatc gtcagcaatt catccatttc
    10741 ttttttcaat cctggactca ttcctttgcc ttcattacca atgactaaag caattgatcc
    10801 tttggcattc catttttgga agtttgtgcc ggtcatatct gtgccaaata cccaaaatcc
    10861 tgtttctttg agctgttgca cagcttgtac caaattcgtc acgcgtgcaa cagggacatg
    10921 ttcgactgcc ccagtggaag tctttgttac gataggtgtg attccaactg ctcggtgttt
    10981 tggtatgatc acaccatcga cgccacttgc gtctgctgtc cgtaaaatcg acccaaaatt
    11041 gtgtggatct tcgatgctat ccaaaatcaa aaagaagggg tcttcttttt tctcttttgt
    11101 tcgttcaatc agttggtcaa gggataaata ttcataaggt gtaatcgcaa gaacgactcc
    11161 ttgatgcacg ccgtgatcgc tcaaagtttc taatttttgt ttaggaaccc atttgactgg
    11221 cacagcttgt tcacgtgcga tttctttcag ttcttctact ttttctcctc gtgaatcttc
    11281 ttgaagaaag agtttattcc ctcgcccttg ttgtagcgct tctactgcgg catggtgccc
    11341 aaagacgaaa ttatcttcta atgcttcttc attttgatca gaaactgact gatttcgtcc
    11401 agtttgcttt ttatttgaat atctattttg ttttttgtct tcaaatccac gtttcgattg
    11461 atgtttgttt gttctttctt tacccatttt tttcaccaac tttctgaata caccagcgga
    11521 tcaattcttc catccgctcg gtttgttttg tcaaatgcag atagcccatg agcgcttcga
    11581 accctgttgc gacccgatac gtcgtaacat cagcattttt tgcgctcgta tggcttttag
    11641 catttcttcc tctgcgataa taaagttctt cttcttctgt caaaagatgt tcttccagca
    11701 tttgctgaat caaaaaggct tgtgcattcg cagaaacata attcgttgcc gcacgatgca
    11761 gcttattggg ttttgtttct ccttgatcaa tcaggtggtc acgaacatag atttcgtaaa
    11821 tcgcatctcc aacataagcc aaggctaaac cgttcaattg tgtgtaatct ctcatgcttc
    11881 tcttctccat cgggttcctt gcggtgtatc ttctaataag atcccttttt ctttcaacaa
    11941 atcacgaatt tcgtcgcttc ggacaaaatc acgattgctt cttgcttgat ttcgttcttc
    12001 gatcaattgt tcaatctctt catctaataa ctcttctgct gtaaataaaa taccgaagat
    12061 cgaaagccat tgcgagaata aagcttctcc ttgtgccaag acagcggcag aaactttcgg
    12121 ctggtctaga taagtgttca gccacttagc aagctcatag accactgtga tcccatttgc
    12181 tgcattaaaa tcatcgtcca tttcttcgat gaatcgctgt tctaaatttt ctaacaaagc
    12241 gattttatcc gcatcatctg ccaaattctc tgaggcagtt tcttggcgga atttcaaatt
    12301 gtcaaacgta ttcttcagtc gttgtaaatt ggtggctgct tctttcatcg tcgtttcact
    12361 gtagcggatc ggacgacggt attgtgtggt cgccatgaag aaacgcaaga cttgtggatc
    12421 aacgtttttg atcatttcat gtacagtgat gaaattccct agagatttac tcattttttc
    12481 atcgtcttcg ccaatcgtta catagccatt gtgcatccag taattagcga attttttccc
    12541 tgttttcgct tcactttgtg cgatttcatt ttcatgatga ggaaattcta aatcttgccc
    12601 gccaccatgg atatcgatcg tttctcctaa atgttttgtt gccataacgg aacattcaat
    12661 atgccaaccg ggacgtcctt tgccccatgg tgaatcccaa gaaatctcgc caggttttgc
    12721 ttgtttccat aaagcgaaat ctaatgggtc ttctttttgc tgctgttctt cccctgtacg
    12781 ctgacttgct ccgacttcca gttcatcgat ggattggtca cttaatttcc cataattttc
    12841 aaatttacgg gtacggtaat acacatcacc tgaagattca taagcatagc ctttttcaac
    12901 caacacttga ataaatgcta aaatatctgg catatgatcc atgactcttg gatgcagtgt
    12961 agcaggttcg acattcaacg ctttcgtatc ttcttcaaat gctcggatga aacgatcagc
    13021 aacttctggt gctgtaatac ctaattcgtt tgctgtacgg atgattttat cgtctacatc
    13081 cgtgaaattc gatacgaaat cgactttata gcctcgatat tcaaaatagc gtcgaattgt
    13141 atcgaaagca atggtgctac gagcatttcc gatatggata taattataca cagtcggtcc
    13201 gcagacatac ataccgacat gtcccttttg tatagggatg aattcttctt tttttctggt
    13261 caatgtattg tagattttta tcattctgat ctcctcactt tttctccttt gattcgaacg
    13321 acttttgctg gtactcctac cgctgtagcg tcgtctggaa catctttcaa cacaacggca
    13381 cctgcaccga ttttggcgtt ttttccgatc gttaccggtc ctaatatctg cgcattcgca
    13441 gagagcatgg ctccttgttt aacagttggg tgtcttttac ccgtttcttt tcctgtgccg
    13501 cctaatgtta cgccatggaa taaaatcaca tcgtcctcga tttctgcggt ttcgccaatg
    13561 acgattccca ttccatggtc gatgaacacg ccagttccaa tcgttgctcc tggatggatc
    13621 tcaacaccag ttaggaaacg ccagaattga gcattgatct tcgccaataa aaagagccgg
    13681 tgctgatata aaaaatgaga gaaacgatgc caaaacaagg catgaaaacc agggtatgtc
    13741 aaaacgatct cagctgtcga acgtgcagcc ggatcgtttc tctttgctgc atcaatcgct
    13801 cgggtaaacc aactcatgac tttccactcc tttcgtaaga attttatgta ggaaatcatc
    13861 ttcttataaa gaaaagaagc gccttttaaa taagtttcct tatcaaaaga cgctttccgc
    13921 gtggttccac ttttcttcat aatcaatgat tatctctgac agctaacgcc tgttatacgt
    13981 tcttggctac tttgtttcct ataaaacagt ttttcctgtt ttctgtgttt cgccaagacc
    14041 actcccagag gcatttcggc aaccattcat tgataccttg caccacccgg tatctctctt
    14101 gtaatgatca gctgcttact tttttctgtt catcgtgttt agttcagtac ttgatttaaa
    14161 tgtgctaatg atttttcacg accaagcaat tcgatcgttt caggcaattc tggaccatgc
    14221 atttgtcctg atacagctac acggattggc atgaaaagat tttttccttt tacgccagtt
    14281 tctttttgaa cagctttgat ggcagctttg acagttggag catctactac ttccatttgt
    14341 tctagctgtg ctttgaatgc gtggagaaca gtaggaactg tttcaccagc aagtacttct
    14401 tttgctgctt cattcaatac aggatgttca ttgaaaaata gcgtagacag atcaacgatt
    14461 tctgcagcat agctcatttg aggttgatac aggctaacga tcttcttcac ccattcgatt
    14521 gtcttttcat ctgggttttc tgctacacgt ccatcagcga ttagataagg cagacacatg
    14581 tcagtaaatg catctagatc cattgctttg atgtaatggt tgtttaccca ttgcagtttt
    14641 ttcgcatcga aagctgcggg tgatttactt aggcgttttg gatcgaacat tttgatcaat
    14701 tcttggtgtt cgaagatttc gtcttcgccc acaggtgacc agcccaataa tgcaataaag
    14761 ttgaacattg catctggtag atagcctaat tcgcgatatt gttcgataaa ttgcaagatt
    14821 gattcgtcac gtttgcttag ttttttacct gtttcactgt tgatgatcaa tgtcatatgt
    14881 ccgaaggttg gtggtgtcca gccaaatgct tcatagatca tcagttgttt tggtgtattg
    14941 gcaatatggt catctccacg taaaacgtga gtgattttca tcaaatgatc atcgaccgca
    15001 acagcaaaat tataagttgg cataccatcg cgtttttgga tgacaaagtc gccaccgata
    15061 ttatcggatt cgaacgaaat agcgcctttc accatatctg taaatgcgta ttctgtattg
    15121 cgaggcacac ggaaacgtac aacagattct agaccttgtg cttctttttc tgcttgttct
    15181 tcaggtgtca agttcgcaca tgttcctgcg taatgaggca tttctcctct tgcgcgttgt
    15241 gcttcccgtt ctgcttctaa ttcttcttct gtgcaatagc atttgtatgc tcgattgcta
    15301 gcaagcagct gatcaatcag cggttgatag atttctttac gttcagattg acggtaaggg
    15361 ccgtattctc caggattttc aggagattca tcccaattca ttcccaacca agcaaggttt
    15421 tccaactggc ttttttcgcc atcttcgata ttacgttttt ggtcagtatc ctcaatacga
    15481 ataataaagt ctccatcatt gtggcgggca aacaaataat tgaataatgc agttcttgca
    15541 tttccaatat gcaggtgccc agttggactt ggtgcatagc gtacgcgtac tttcgtcatt
    15601 ttcttcctct tctttcaaac tagactttga ctaacagcaa aaattccgtc agttacggct
    15661 tctcaaatat catttattta aagccactca gcacaaattt taccttttct aaggtaaata
    15721 gtaaagccaa ctggttgaat ttccttacca gttggctgaa tttctttttt attttgattg
    15781 tttagtcttt ttctctgtct cttttttatt gtcgattcct cgtcctgcat gtgcaggttt
    15841 agcaaagatc atgcgtccag cagctgtttg aagggcactt gttacaacga cttcgatacg
    15901 ttcattcatg taatgctgtc catcttctac gacaaccatc gtgccgtcat caaggtaggc
    15961 tactccttgt tggcgttctg tcccggcttt tactactaga acattcatcg tttcaccagg
    16021 gatgacgact ggttttacgg catttgccaa cgcattgata ttaagaacag gtacattttg
    16081 gaattcagag actttattca agttatagtc attggttaca atgacaccat ctaaaagctt
    16141 agccaatttg atcaatttgc tgtctacttc actgatatct tcaaagtctc catcgtacat
    16201 ttcgacagat ataccctctt ctttttgcaa ggcatttagg atatctaatc cgcgacgacc
    16261 acgcacacgt ttcaaactat cccctgagtc cgcgatgtat tgtagttcgt aaagaacaaa
    16321 attagggatc atcaaggttc cttctaagaa tccagtcttt gtaatatcat aaatccgacc
    16381 gtcaatgatc acgcttgtat ccaagatttt atatttatgg aaattttctc ctgattttcg
    16441 ttctaacagt tgactctcaa cttgactagc tgtttcttgc tcgatttttt tgcttcgtga
    16501 accaaaaact ttcttccatt cttcgattcg tgtcgttccc attctcaatc ccagatagcc
    16561 taagatgatc atcagtaaaa tcggcaatac actattgacg aatggaattt ccatattgta
    16621 aagaggggtc gagatcacaa cacctaaagt caatccgatg atcgcaccga tcgcaccgaa
    16681 gagaagataa gtcaagctga cctcacttag tttttgttct acttgtttga taatagctga
    16741 gatatgtttg gccaacagca aagataataa aaagaaaata agtgcgccta acaggctgtt
    16801 ggtaaatgta ttattcagcc atccgtttga ctgttgaccg attgcctgcc aagctgaggg
    16861 taaaaatgaa atacccaagc ttgcgccgac aatgatcatc agcaaagtaa tgacacgttt
    16921 ttgcatcgtt tttcctccgt ttcgacaatc atttattagt tagcggaaca cacgcttcaa
    16981 cgtttcagcc aaagttgcta ctccaactat ctcgattcct tttggcgctt cccagttacc
    17041 taagttgttt ttaggtaggt atacttttgt gaagccgagt ttttggactt ctttgactcg
    17101 ttgttcaata tgactgacac gtctgatttc tccagtcagt ccaatttcac cgatgaaaca
    17161 ttccgttggc tgtgttcctt tttctttata gcttgaagca atactgacag cgatagctaa
    17221 atcaatcgct ggttcattta attttacccc acctgcagct tttaagtaag catcttgatt
    17281 ctgaagaagc aacccagctc ttttttccag cactgccatg atcagagata cccgattgaa
    17341 atcaagacct gttgttgttc gtttggcatt tccgaacata gtaggtgtga ctaatgcttg
    17401 gatctctacc aaaattggac gtgtcccttc cattgccaca acgatagcag agccagttgc
    17461 accgtctagg cgttcttcta agaaaatctg tgaaggatta gcgacttctt ccaatccatg
    17521 ctcatgcatt tcaaaaatcc caatctcatt cgttgagcca aatcggtttt ttactgctcg
    17581 caaaatccga aaactatgat gttgttcacc ttcaaaatat aacaccgtat ccaccatatg
    17641 ttctaacatt cttgggccgg cgatcgagcc ttctttggta acatgaccga cgataaaaat
    17701 ggctatccca tttgttttag cggttttgag tagttctgca gtggtttctc ttacttggct
    17761 gacacttcct gccacacttg tgatgtctgg ctgcgtcatc gtttggatgg aatcaatgat
    17821 aacataatca ggggagatat gttcgatggc acgactgatt tcgttcatat ctgtttcagc
    17881 gtatagataa aattctgtgt tgatacttcc gaggcgttcc gcgcgtaatt ttatctgctc
    17941 tgcgctttct tcgccggata catataagac ttttccgcct atggctgcta attgctgaga
    18001 aacttgtaac agcaaagtgg atttcccgat acctggatcg ccaccaatca agaccataga
    18061 ccctggcacc acacctccgc ctaacacacg gttcagttct tctaattttg ttttgatccg
    18121 aggttctttt ttagggacga cgtctgcgat ttttgttggt tttgcttttt ctcctgtcaa
    18181 acttgtccga gttctgcggt ccgttgtatc ttgttcgatt tcttcgacca tcgtattcca
    18241 ttttccgcaa tttgggcagc gtcccaagaa cttaggcgac acatacccgc aattttggca
    18301 aacaaattgt acttttgatt tttttgccat tgtaccgcct ccttttatcc taatcatgag
    18361 acattaaatc aagcttaagt atctttaacg ttttatttac tggaagaacc aaatccgcct
    18421 tgacgttgag cagtcgtttg atcttgatct gcttttaaaa acggcaaaaa gatcccttgg
    18481 ccgatacgct cgcctttttt gatcactaca tccttgaaac caaagttggt gaattggaac
    18541 atgatatggc cttcatttgc ctcattattg taataatcac tgtcaatcac acctactccg
    18601 tttgtcaaca ctaaaaaccg cttcaacgga ttgcttgaac ggttagcaag ctgcaaaaat
    18661 tcatcttctc ccatgtaagc tttgattcca gtaggcacga gggttggttt caattgtttt
    18721 tcattttctg atacatcttt tttcaatcca tctgcgactg tttccaaccc ctttttccag
    18781 cctgctttcc agatacttgg aatcacgacc tcttctgctg cttcaaaatc atagccagca
    18841 gcatgatgtg tagcgcgctg cggtagattg atattttgat ctttatattt agaaataact
    18901 tcaaatcctc tttttctcac cggttttcct ccctaaataa actactccta ttctaacata
    18961 tccgaggctg taaaagactt tctttacaaa ttcttcaaga aattctgcta tcttaaagta
    19021 aaccattaat tcgttctttt tccaaacgac tgcaagttgt acaaaaaaac tattttttac
    19081 atgactttac tcgtcaaaac aagtagaata gaacgaaatt aatgagtgga tatttgattg
    19141 aggtgtcaca tgcgtaaaga aattaaaaat gcaaaacgaa ttattgtcaa agtaggaaca
    19201 agtacattga tttatcccaa tggaaatatc aatcttgcgg caatcgatca gttagctttt
    19261 gtcctttctg atcttagaaa tcggggaaag gaagtgatct tagtctcatc aggagctgta
    19321 ggcgttggta tgcataaatt acatttgtca gaacgtccga ctttgattcc tgaacagcaa
    19381 gctgcggcgg ctgttggtca agccgaactg atgaatatct ataatcaaag attcagtgta
    19441 tataatcaac agacggcaca gatcctcgtg acaagagacg tggtagatta tcctgaaagt
    19501 cgtaaaaatg tgaggaatac attggaacag cttttgatta tggacacgat ccctatcgtg
    19561 aacgaaaacg atactgtagc agtggatgaa ttagatcatc tgacaaaatt tggcgataat
    19621 gatcagctgt cagccatcat ggcaaaactt gtcgcggcag accttttgat cgtcctttct
    19681 gatatcgacg ggttttattc agataatccg actacaaatc ccgaggctca gatgtatcgc
    19741 tatatcgata aaatcgatga aaagattctg gcacaagcag gtggtgctgg tagcacattt
    19801 ggaacaggcg gtatgcagag taaattaaaa gccgcttcta ggatatttga agcaaacagc
    19861 gcaatgattt tagcaaatgg aaaaaatccg gcaattattt ttgatattct tgcaggaaaa
    19921 gaagtaggta ctttatttaa ggaggacttg aaatgactga cttgatgcaa ttaggacaac
    19981 aggcaaaaga tagcgcgcaa caactagcac ttatgcctac aaaccgcaaa aatgagctac
    20041 ttgttcaaat ggcacaaacc atcaaaaaga atcaacaagc gatcattgaa gcaaatcata
    20101 aagacttaga aaaagcagtc gaaaacaata tcagtgaaac gatgcaagac cgcttgcagc
    20161 tgacacctga gcgaatcaat gccatggcta cagagatcga aaaaattgct tcgttagacg
    20221 atcctattgg aaaagtagat gagatgtgga cgaacaccga cggattgaga atcggcaaaa
    20281 agcgcgtgcc actaggcgtg atcggcatca tttacgaatc tcgtccaaat gtcacgacag
    20341 atgcagctag tctagcattc aaatcaggga atgccgttat ccttcgcggt ggcaaagaag
    20401 cattcttctc gaatcaatta ttagtccaat tattgcagca agtactggtc agtgctggcg
    20461 aatctcctta tgccatccag tttgtcgatg atacttctca tgaaactgca ggacagttga
    20521 tgcgattgac agaatactta gatgtgctga ttcctcgagg cggagcaaaa ttaatccaac
    20581 gagtcaaaga acaggcaacg gtacctatca ttgaaacagg tacaggaaat aatcacgtat
    20641 atatcgataa agaagcacag ttatctatgg cagtgaacat catcgtcaat gcaaaagctt
    20701 cccgtccatc tgtctgtaac gcggcagaaa cattgctgat ccattctgag atcgcacctt
    20761 ttttcttgcc agctattgaa aaagaactgg tagaacacgg ggtctcttta cgtgcggatg
    20821 ctcgtgcgtt agaatatcta gagacggctg ctttagcaga agacacagat tgggatactg
    20881 aatatctaga ttatatcctt gctgtcaaag tggtcgattc tttggatgaa gcaattgcac
    20941 atatcaatcg gcataatact aaacattcag aaacgatcgt gacagatagt tatgcggcta
    21001 gtcaacgttt cttaaatgaa gtggacgcgg cggctgtcta tgtcaatgcc tccactcgtt
    21061 ttacagacgg atcggtattt ggttttggtg cagagattgg tatctctact caaaagttgc
    21121 atgcccgtgg tccaatgggg ttaaatgaat taacttccac aaaatatatc atttatggcg
    21181 atggacaaat ccgcgaataa tgtttcgtgt gaaacattat tcccgccttt tgcctttttt
    21241 ctgtaaaggt tctatccaac tagaaaggtg tcaatatgtt tgaaatttta atgatttttt
    21301 ttatttacct tatttctctg aatatcgctg cttttctggg agtcggtatt ttatcccttt
    21361 tctttcaatt caaaaaacga agcataggga gtcaacggga aaaatggtcg cagtattttg
    21421 acaaaatcgg gcaaaaaggg ctggttacac gcttgcacat tagttacatg gttgcattat
    21481 gcttattagc gacaatcaat tactattctt ttttcgatca ttcaatcgct tatacgatta
    21541 cgctactgat tgctgggatt tttcacttat cttataaata tcagctgaat aaaaaccact
    21601 tgaatcggac tttccgctaa aaaatatccg tacgataagt agagtgcctt gaagatcggc
    21661 aaaaaactgc cggacttttt agaggctgtg acaaagattt tgtcacagcc tctttttcca
    21721 tataaacggc gttcgcctac cagctgcctg gtaaatctgt ttcctcttcg ttaagaagcg
    21781 aaaatccgtc aaatgtttcg ccatcataat aacgaagcag ctcatccatg gacaagttca
    21841 tcgttttttc tttttcttct tccgctgatt cagccagtag ctgttcccga tacacctgat
    21901 acataccagc tattttttgc tgcatcgctt ttctagtttc accttccgca ttgattattg
    21961 gcaattcttt gatatagcca cgataaatca accgatcatc tgaaaattca atgggtaatt
    22021 caacgagtgt caacccgact tcctgaaaat attcttccag catctattat ccccccattc
    22081 tttttcttaa atgaatttta gcttacctac tgaaaatttt ccagtttcat tattttgaga
    22141 aaaaaaggga atgtgacaaa aatcgtgtca catccccttt ttccgaataa atggtgttct
    22201 tctgccaact cctcaatcat aagtcacaat attcaacaaa catgagaaac tgttttttga
    22261 aattgttcct tgtgattcag agttaaacga cagtctcaca acctcttggt aaacggcgtt
    22321 caatcagctg accctcggta ttagctcaaa aaacggtaaa atatgagaag ctatttttcc
    22381 gttttgtttc tctaatactt aggataaaac gctgatttta caacctctgt taaacggtgt
    22441 tcaatcagct gtccttcggt attagctcaa aaaacggcaa aatatgagga actatttttc
    22501 cgtttgtttc tctaatactc aggacaaaac gctgatttta caacctcctg ataaacggtg
    22561 ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gaagctattt
    22621 ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc tgatttctta
    22681 ttcttcttca tttgtagcaa aataattacg gtgataaatg atacaacata cactgataag
    22741 aacaatggca cctgcaaaat aaaacgcata gctttcacca atatgttcac caatcacacc
    22801 tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca aaactatagc tggacgaagc
    22861 aacgtttcgt tgcagacctg gtatttccat agcaatcgtc gtcaacagtg tattgataat
    22921 tccttggaaa aatcctgcta acacaatgct gatcggtaca aggactggca tagaagttcc
    22981 agctcctaat aacagcaaac aaataatgaa taagacaaat gctgctaaaa ttgtccgata
    23041 agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc gaagcaatag ccagaagcac
    23101 accccagcca aaaaatacaa aaccgacttc taattcagaa tagccaatca ataaaaatgg
    23161 cgcatacgca agtaaagtaa agaatccgaa attatataac aatgcgatta gtccaactga
    23221 acgaagttta tgattgctta acgctttgac accttcccaa aggcctactt ttctctttgg
    23281 tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa ccgcaaaaca tcaaaaatgc
    23341 taccccaaaa aatggataac gccatgaacc actgcccaag accccaccga ctagagggcc
    23401 aacagccatt ccgcagccaa gtgctgcttc atacatcatg attgcttttt ctgtgttacc
    23461 aactaaaata ctgacgatcg ttgctaaagc agtagagaca aataaagcat tcccgacacc
    23521 ccagccggca cgtaacccaa tcagcattcc gatattccca gataatccgc caagaccagc
    23581 gaatacaacg atgataaaca atcctgtcat caatgttttt ttcaaaccga tccgactaga
    23641 taagaaacct gtaaatagca tcaccacacc agtgactaac atgtaactag taaataacag
    23701 ggtcgtttca gctggcgtag cgtttaactg tgcagcaatc gtttttaaga ttgggtctac
    23761 tagacccact cccataaaag cgatcataca ggtagctgct acaactagcg ctgaactttt
    23821 cccctttgtt tcttcatttg tttgttcttg tatatctatt tgattctcca atatttccat
    23881 ccctttctct ctattagcat catacatgat ttattttaca tgtcaatatg cacatgtaaa
    23941 acgtggaaac tttactgtga tataataaag aaaataagac gagaggtagg atttttatga
    24001 atactttagc ctatatatta ttatgcgtgc ttttatccaa accacgttct gggtatgaat
    24061 tgaagcagct gatcacaatt ttttgggaag cccatcacag tcagatctat acaacgcttg
    24121 ccaagttaga ccagcagggt tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa
    24181 agatctacca cttgactgat gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa
    24241 ctcccgctcc tacgcaaaaa gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg
    24301 ataaaacaac agctactgga ctgctattca ctagagaaca acagctgaac aaaatcatgc
    24361 aaaaaaatca aaaaaaattg aatgacctct cgctagacaa gaaacaagaa tttggacgtt
    24421 atgttgtgat ccagcgcaga attatgcttt gtcagcagga actccaatgg tgtcagcaag
    24481 taagagaaca aatgcagcag ttttttgagt aagggataaa cagaaccttt ctagaaatat
    24541 ctattgaaga gcaaattatc ttcaataaaa gagcaataca gttggaaaag aaaaagcgat
    24601 ccgaacgaca ggccaaaacc tatcgttcgg atcactattt ttctagaact gcattcaaga
    24661 gactatgaca aaaagcatgc caaggccttt tttcaaataa cagtgttcca tcattcatta
    24721 ctttgaattg cttttagcac acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt
    24781 aaagcggcaa tcacattcat tttccaagca ttcatataag cagagaataa ttgatcaggt
    24841 attccttgtt ccatgatcac accgaataaa gacatacatg tcaccattcc taaaaccatc
    24901 aagcatgaaa tagttaagat catttggacc ttgctttctg gatttactgg tagtttaaag
    24961 gcaatttttt tcgccagcac accgatgatg aagacatcca aaatgaaagc caccacgaat
    25021 ccaggcacta gaccaatcaa caaacttgat aaatgaagat cttgatgcag ccataaatta
    25081 tacgcgctca tccccaagac catcaaaaaa cacatcattg ttgtaaaaat tattccttct
    25141 tttttatttg taggcaatct gtaccactcc tttaaatttg tcacgaaaat catcgtagca
    25201 catttaaaaa tttctcgtaa gtactttttt tattttctgc ccttttattt cataaacttc
    25261 atcagccgta cgctcgacaa agtaagaatc atgagaagta aacaaaatcg ttcccggata
    25321 tatttgcatt agcttctcta aagcttcgat cgttgccata tcaataaaat tagtcggttc
    25381 atctaacagc aacacattac ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc
    25441 ttctccccca cttaatgtac ataacggttt cgtcaaagtt tcctgagcaa accctaagtg
    25501 attcagcaaa ctacggacaa gcgattctga aaactctgtt tgtctcatga caaaacgaat
    25561 gatggattct tcagatgtca tttgataacc cttttgctga tatacttgaa agcttacttt
    25621 cggagagaga aggattcctt ctccttgttt gcggatatgt tctaataagg ttgtcttacc
    25681 tgaaccattt tcaccgacaa tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt
    25741 taccagtaat gttctcccac ttctttccaa ttggacattt tgtcccatga tgggatagcg
    25801 gctgtggagt tcaagagatt ttggtactgg aaaacggatt tgtttcatct gctgcggctt
    25861 ggttgtttct cctatttttt ggagtcttct ctctaatgtt ttcgcattct tttggatggc
    25921 tttttgtaca ctgtcttttt gtttagagga agacaaccga tccagtctga tttgttgctt
    25981 tttcttttta ctggacactt tttccaactt ttccgcttgt tttttctttt cttggatcga
    26041 tttttccaat cgttttttct ctttcatgat cgactctgct tctcgtagct gagtcttttt
    26101 ctccaattcc ttttgagtga gataggcact ataattcccc gaaaactctc ggatttctcc
    26161 gtcttttact tcccagattt tcgatgctag ctcatctaga aaatagcgat catgactaac
    26221 aaaaaccagc gtaccataat aataacgaag ctcttcaatc agtcgatcaa tactttttct
    26281 atctagatgg gttgttggct cgtctagaag catcccttgt tcataagaag aaagaagacg
    26341 tatcaactcg atctttcgtt cttcccctcc gctccatttc cgctctgaag tttcttccag
    26401 tcttaactgg aaatgactgg ctaattccaa atttaggtcc tttgcttcag cgagataggt
    26461 taattgaggc aggtagttga aggtgatctc tgtttggatt ttcccatgat cgggaaaaag
    26521 ttcaccggct attagtttga gcaaagtcga tttaccgaca ccattttctc ctatgatccc
    26581 tatacgatct tgctgataga cacgaagaga atcaatagat agttctagct ggttgccaaa
    26641 gctgacttgt agatttgtta tatttactgc taaattttcc atatgtttta cactcctatt
    26701 tcatgtaaaa caacaattat cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa
    26761 ctgcatcaca tcttttccta ctctgaacga aataactatg actaaatcac caaatctgtc
    26821 atagctttca cctattctac tgtcgttcga aaaaatggac atactccatc catgaaattg
    26881 ttgtttatac atgtcttttg aatttattta gttgttattc aaaagttcga aacgtaattt
    26941 catcgatgca gtcatttttc cgacctcctt ttttattagg ttaccacaca cgattcaaaa
    27001 aaagcaatag ctactgaata gtaactgaaa accttttatt cgaacatagt gatcttttct
    27061 ggtgctaaac gaacagactc gtaaccttct tctaccgctt ttcctattga taaaataata
    27121 actggaacat aacggtcttt atccaaacca aatgcctctg ccaactgatc tgcttcgaat
    27181 ccaccaattg gatttgtttc atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc
    27241 gctaagctag aatcgacttt gactacatcg ttcatttctt ctcttgaaaa actcttgtaa
    27301 taaggaatga tcgcagctaa ttgttgatct ctgacttctt ttggcatctt ccctgattcg
    27361 taagcttggt tgtagatttc ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg
    27421 accattgcag aagaagtatc attttgtctc gtattgaaac ggatcaaagg acgcaagatt
    27481 tcttttgctt cctcgctttc agcgaccaca aagcgccatg gtttcatatt gacagaagaa
    27541 ggtgcagtcg tcgcttcttt gatcatttcc agcatttctt cgtgtgggat tttgtatgtt
    27601 tcatcatata cgcgaacgga tcttctacca gtcataattg ttgaaaaatc atttttttgt
    27661 tccattatta ttaacctctt tcgtttctgt tgttatattg caaagttggg tattcaattt
    27721 atctaaaagt gttagaaatt gtttcatttc tacatcgctg aaagccgggg atagcacgga
    27781 acctgtatct ttatggtcca tttcacaatg cttgattttc ttctgggctt tttctgttag
    27841 cgctacatac acttcccggt tgttctgagg atttctctga cgcgaaacat agccatttgc
    27901 ttccaatacc ttcagatgac gagtaactgc tccttgatca atctgcaact tttcttgcaa
    27961 actgctttgt agcaatggat tttcttcttt cagtctcatc atgatctcgt atctagtaag
    28021 acttataccg atattttctt caaagatctg gacgattttt tgatcggcta atttgatttg
    28081 atataaaagg tgactaatct cttctaaaga cataatttcc tcctttctaa taattgactc
    28141 gtcaacgatt gacttgtcaa taatagaata aaaaaagaaa aaagtcaaag aatcagctgt
    28201 gtaataaaaa aaacgctctc aatagaaaag tatagagaaa agaaattttt tgtagaatga
    28261 acggaaaaaa tgataaagaa agcacaacgc taaaaccaag attattgatt cttatcaaac
    28321 tatagaagat agctcttctg cagtagataa ttttacaccg ccaaaaattt ccgaaatcgc
    28381 cttttgcaag aatcccgtcg aatattagaa attggatcag tatgcttaaa cagtaataaa
    28441 aaagttatga caagatgttg tcacaacctt ttttgagtaa acggtgttca aaaacttgca
    28501 tctgcggtaa taagttcaac caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc
    28561 ttactacttg atgcagggca gctttttcac tacctctttt taataaccat aaaatcctat
    28621 tccttcatat ctccagccca tcggtgttag ccaatctctt tccttagcac tcaaggtata
    28681 gtgatgggta ccagtttttt catttggatt aaatagtcga taaattggct tggaaccacc
    28741 agaataaaaa cttagacctt cataacgcca accgattttt gttaaagagt tgatctcaga
    28801 ttgtgccatt gtgtaatgat ggtctccact gtaaggattg tataaacggt agacaggttg
    28861 tccgctgttt ggtgcttgcc aagcaatccc ttcatattcc cagcctacag ttggcagata
    28921 atttttttct ccggcattca atgtatataa atgttctcct gtatgacggt tatacagacg
    28981 atacatcgga gaagttgaag ttggtgctgt atcatactgt gtcagattat aggtttcgat
    29041 tacattgttc aatgtagaac cataattagg cgcagtggca taacgacctg ttagccaagc
    29101 cgtagcatct ttgtaagagt tcgtattgct tttccatgca ccggaatagt agtaaacgcc
    29161 tggctgaaaa gaagtcgtct tcaataccgt tacgttatct tgcaatgatt ctttgtacga
    29221 aggatatttt ctaaaagcgg cattcaccgt aatccattgc ccattcacat attccgaagt
    29281 aggcatcgta acagattggc cattatagct tcctttaatc ccaaatagat tgtaattagg
    29341 ggcagaagcc aaagtactgt tcccccagcc actttcaacg atggcttgag caatcatgac
    29401 tgatgcatat aagtcattgg cacttgccaa actttgtgca gttgaggcaa tactatcaat
    29461 gaacgcttgt tgagaattag cagatgagcg agcataaaaa gcatagccag tatagttttc
    29521 gaattcagac aaatctgctt gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt
    29581 cgtgatttct tctgcattct ctggattgtt ctgagcttct gctgatgtac tgtcttcggt
    29641 ttgtgtttgt tctgttgttt cagttgttgg ttcttcggtt gaaattgtat tttctgagac
    29701 agtagctgac tgcaaagtac tttctgtatc gttttcaatt cctgtttctg aagaactcga
    29761 tgtctgagtt gcgacagttt ctgtcgattc ttcagctagc actgttgtgt tggcaactgc
    29821 tagatttcct aaaagaataa cgctaaataa taaattcttt gctttcatct tttttctcct
    29881 ttgaattatg aaacttttcc taaataacag tgtaactaaa aaagtaaaag caagtcaatg
    29941 tccatattta aataacattt gtttcttttt aataaatttg ctttttctgc ataaaaagac
    30001 gtaaatgaag aatattcttc tccattcacg ccttaaattg atggtcgttt gaataactat
    30061 ttaacaagta attctctctg gatattatcc gttatggatt atacttcctg tgtcgtcgga
    30121 cggataatca tgttgctgac agacatccgg tctggcgttt ctatcgcaaa tgctacagca
    30181 gaagctatat cttcagatgt taatccccat tctttttgcg cttcatgaag ttcaagagca
    30241 gcctttttgt cgctgatggt ttgatataat tcagtttgaa cagctcctgg agagatgatc
    30301 gtagatttga tgttattttg ccgttgctct tgacgtaatc cttccatgat tgcacggaca
    30361 gcaaattttg ttccgcaata aacggcggaa tcaggataaa caacatgtcc cgccacagaa
    30421 tcagttgaaa tgatatgccc agatttttgt ttttccatga ttggaagtac ggcggaaatt
    30481 ccatttaaca cacccatgat gttgatatca agcatatttt gccattcatc acggtgacct
    30541 tcaactaatg gagcggttgg catgattcct gcattattgt acaatacatc gattcgaccg
    30601 aacttttcca tcgctaaatc gataactgct tggacttgtg caaaatctct tacatctgct
    30661 gtttgaatat atagctcagc ttctggtaaa gattctttta ttgcttttag gcgatcttct
    30721 cggcgtgcag ctataactag tttagcccct ttttctgcta atagcttcgt tgttgcttca
    30781 ccgattccgc ttgaagcgcc catgatgaca atcacttttt ctgataaatc attcattcta
    30841 ttctcctcct gttttgaaaa atctcttgct tatactcact atctcttatt tcatcttact
    30901 gttcaaataa tccgttcgac aggtttgacg attttggcag ggattcctgc aacgatggta
    30961 ttgtctggga catctttcgt aacagttgca ttcgctgcaa caatggcatt ctcgccaatc
    31021 gttacgcctg gcagaatagt tgctcctgct ccgatccaag catttttctt gatgtggatt
    31081 ggctttactg acaagcctct tcgttctttt ggatcggtta gatggttcac cgtgatgagg
    31141 ttgacacgag gaccaatcaa tactgaatct tctaaacaga tgccacctaa gtcaacgaac
    31201 atcacttgac gattgataaa gacatttttc cctatactaa tatgtttgcc gaaatctgta
    31261 taaaaaggca gcagaacagt caccgtatca tctactttgt cactaatgat attttctagc
    31321 cgtttgcgga tatctgctgg ttcatggtac ccagtattca gcgctgctaa aggctcttcg
    31381 ttttccgctt ggatctgatg gatcacttca aagagcggat ccgttactcg aatctcttta
    31441 ttgacgattt gtttttcgat gaggcttgtc attttgctcc ctccttttat ccttacttta
    31501 taacggacgg cttatgatgt ctaatactta taaaacatac ttaactattc cttttaacta
    31561 tacttagtgg tatgcttttt taaaaacagg agggaaaaaa tggatattcg tatactgacg
    31621 tattttttag cagtagctag agaaaaaaat atcagccgag cagcaaaatc tctgcatatc
    31681 acgcaaccta ctctctcaaa acaactgaaa gatttagaag aagaacttgg tgtgacactt
    31741 ttcactcgag gctcccgaga gatacagttg acggattctg gtcgatatct ttattcaaaa
    31801 ggaaaagaaa tcttgtcgtt ggtagaaaaa acggcgaaca atttaacaaa tgacgcaatc
    31861 atctctggag aaatctacat tggcggaggc gaaactaaag caatctcttt catttctgat
    31921 agccttcatt cgttgatcaa ggcgcatcct gatatccagt ttcatttata tagcgggaat
    31981 gcagatgata tcatagacaa actggatagc ggattacttg attttggact cgtaatcgaa
    32041 ccgacaaaca aacaaaaata ccagtcattt cgactgccag cccaagatcg ttggggactt
    32101 ttgatacatc ggaatcatcc tttagcaaag caaacagtca tccatccgca ggaactgaaa
    32161 actgttcctt tgatcatttc ccggcaaacg tcagtggaca gtcaactagc tgaatggcta
    32221 ggtttcagca tagatcagtt atcgatcgtc ggaacatata acttattgta caatgcttct
    32281 ttgatggtcg ataacgggac ttttggtgca ttagctctag atgggatcat caatacttat
    32341 gggacggaaa aagtattcat cccactcgat ccgccattac aagcttcact gaatctcatt
    32401 tggaaaaaag accagcctct ttctcctgct gctcgtaaat ttttagatgt cctaacggag
    32461 aaatacagtt aagtagagtt tttttccttc gctttcagct ttttaacaac gaataagtta
    32521 agcaaaagat ccaatgatta aataacataa gtttcttaat ttgttccttc cttttatttc
    32581 tgataattcc agtaaattac tataattata aaaaaagaat tgacaaaaca taactgtcta
    32641 tataaaattg attacgttaa caataattcg tttcatgtga ctagatcata ctaggcatga
    32701 aaaatctctg aggaacatgg tttccctagg aggtttttcg tgtctatttt tgtttggccg
    32761 caatcaactg gtctagcaaa cgtgaaaacc aaatcttcaa aaagggtgcg cgcatgaaaa
    32821 tcaaaaagat ctttaatcaa aatgcagtat tggtagatga cggtggacag gaaaaagtag
    32881 ctattggaaa agggattgga tttgacagaa aaagaaatga tttgattttt gatcgtgatg
    32941 tcgaacggat atttgtcatg gaaccagaag gtcaaatcaa gttgcaaaat ttacttaatc
    33001 agattgatga acgcttttta tttgcagcag aaacgatcat tgatcatgcg gaaactgttt
    33061 tgatggaaaa gttgaatgaa catgtattga tcgctttgac cgaccacatt gctttttctg
    33121 cggagaacat taataacggt atcgtcatcc gcaacaaact tttaagagaa atcgaagtgc
    33181 tttacagtga tgaattcagt attgctcaat gggcagtaga gtatctgaca aaagagcttg
    33241 ggattccata tacttatgac gaagcaggat atatcgcaat ccatattcac agcggacgaa
    33301 gcggtcggaa caataaccat cgcagtatcc gtgaagtgac gattgtatct gacatcatcc
    33361 atttagtgga atcagaattg gaaacagata tccattcaga accattttca ctgaattatt
    33421 caagactggt gaatcattta cgactgcttc tacaacgaac ccatgcgcaa caatatgctg
    33481 ttttagacac ggaaatcgta gaaatggtca aacgaaaata cccagaaagc tataagattt
    33541 ctaaaaaaat ccgtgtgttg ctgaccaaag aataccagct cgcgatcacg aaagaagaac
    33601 tgggttactt ggccatccat atcgagcgtt taagaagcgc aatcgtacaa acaaatgtta
    33661 acaatcatga ggaggaaaaa aattgaaagc atatctacaa cgaatgggac gttcccttca
    33721 acttccagtt gcggtcttac ctgctgctgc tttactagta gggatcggaa actggtgggc
    33781 atcttatagt aacgacattg tcgctcattt tttacaagct ggcggtaacg cagttttagg
    33841 tcaattacct ttgctgtttg ccgttgggtt agcactaggg atgtcagtgg acaaatcagg
    33901 tgctgctgcc ttggcaggtt tggtcgcatt tgaagttcca gtaaatgttt taaaaactga
    33961 ttcagtagct actttattga atatcaaacc agaatcagtt gatccatctt ttgctcagat
    34021 cagtaacgta tttattggta ttattgccgg attgattgcg gcagctcttt ataatcgttt
    34081 ccacgaagca aaattaccga tggctttatc tttctttagc ggcaagcgtc tagtgccaat
    34141 cttggctgcc tttgtcatgt tgctgatcag tgcagtgcta ctgctcgtat ggcctccggt
    34201 ttactctgct ttagtttctt ttgctaaatt cattttgagt ttaggctggg gtggtgcagg
    34261 tctttatggc ttcttcaacc gtttactgat ccctacagga ttgcatcaag caatgaactc
    34321 cgtgttctgg tttgacgtag caggaatcaa tgatattggt aaatttttgg cttctgaagg
    34381 aacaaaaggc gtaacaggaa tgtatcaagc tggatttttc ccaatcatga tgttcgggct
    34441 tccagcagga gcatatgcta tttacagaaa tgcacgtcca gaaaacaaag caaaaacagc
    34501 ttccttgatg ttagcagcag cttttgcttc attctttact ggtgtaacag aaccacttga
    34561 attctcattt atgttcgttg cttggccatt atatgtcttg catgcaattt tcactgggat
    34621 ttctttagca gtgagtgcat tcttccattg gacagcagga tttgccttta gtgccggatt
    34681 tgtcgattac ttcctatctt tgaaaaatcc gattgcgaat cagccattga tgttgatcgt
    34741 ccaaggatta gtaacagcag tgatttacta ctttggcttt aactttgcta tcaaaaaatt
    34801 ccatctgatg actcctggcc gcgaggaagc agatctttca gatgacgata ctgcaacgac
    34861 taattcagac aataaatatg cggcacaagc aagcaaaatc tacgctgcat taggcggagc
    34921 cgacaatgta acttctatcg ataactgtac tacacgcctt cgtttgcaag tcaaagatac
    34981 aggcctgatc gatcaaaata aaatcaaagc cactggcgta cctggtatga agatcatcga
    35041 cggaaaaaat gcgcaagtca tcgtaggtac agaagtacaa ttcgttgcag atgagatggc
    35101 aaaactacat ggtggtgctg caacacgacc tgcacaaaca aacaccgtgg ttaaaacaga
    35161 aacatttgcc ggtggaacga tcgaacaaga tatttacgct atcgcaaatg ggaaactgat
    35221 tccaatcact gaagtttcag atgatgtatt cgcggaaaaa atgatgggtg acggctatgc
    35281 tgtattacca gaaaatggag aaatcttctc tccaatcgct ggtacgatca caaacatttt
    35341 cccaacgaaa catgctgtcg gaatccaaac agatgctggt atcgaagtct tgcttcatat
    35401 gggcatcaac actgttgact taaaaggcga accattcact ttatatgtaa aagaaggtca
    35461 aaaagtcgca agaggtcaat tgattgcttt agttgatctt gccgctattc aatctgcagg
    35521 caaaaacacg gatatggtcg ttgtattcac aaatggcgac aaagtccaaa gcttagaaat
    35581 cgaacccgca cgtgatgtaa aagcaaatga taaaatcggc tctgtttcca acaaagcctg
    35641 agccatcgtc ttccccagac ttttttatcg ataaacaaac aggagctgag acaaaaatct
    35701 tgtctcagct cctttttccg aataaaaacg gcgttctcct gccaactccg cggtaataag
    35761 tccagccaaa cgtaacatat gaagagctat tttttatttg actcttctta ttacttgatg
    35821 cagaacagct tttttacaac ctctgataaa cggtgttcaa aaaccagttt atcggtatga
    35881 agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt catactcaaa
    35941 actaatcggc tttttcacaa cctctttata cattattcct gatccaatat ttcttttaat
    36001 gctgcttcaa gagaagttaa tggttttccc aatacttttt ctaaatcatc agaggaaata
    36061 gctaactgat cctttttaat atcatgttgg atcgctaaaa acatgtcagc tgcttcttga
    36121 gggaatccgg cttcttttag attttcgaca aacgctgcat cacttgcttc aataatttct
    36181 aactttttac ctgtcgcacg ctctaatgcc ttggctaatt cttcatagtc agttagattg
    36241 ccggataatt ctaagatttc tggagagtca ctttctacta gtacttttgc tgcagcttct
    36301 gcatattcac gtttcaatgc ccaacctgtt tttccatttc ctgcagcata aacgaattga
    36361 ccagttttta aagcctgccc aatgataggt aattcattct ctacatacca attattccgt
    36421 aaaaatgtat gggaaatacc tgaatcctta atcatttttt cagtgaattg gtgatctggc
    36481 gctaatacac tgatcgagtg atcggcatct gcaaaacttg tataagcgat ataagatacc
    36541 ccagctgctt gtgctgcctt cactacattc ttatgttcgg cttgacgatt tcctggaacg
    36601 ccagagacaa aaagcaatcg atctattcct ttcaacgctt ctttcattga gtctaaatca
    36661 tcataatcac caatacgtat attaaatcca gcttctttca gtgcttctcc tttttcttca
    36721 ctacgtacta gtacatagat ttcattttgt ggaaccatct tttttaagaa ttccagcgca
    36781 taaccaccaa aacctccagt tgctcctgtt actaaataat gcatatttgc cactccctat
    36841 tctttttatt tgttaagatt tacagctgta catttataaa ttacaccttt atcataaaga
    36901 gtgtggtaag ataagtcaag caaaaaaaat gaaaggatga caactgatga agtattctct
    36961 gcaatttagt gatgcaatcc atgtacttgc ctatattgaa atgtttcaag atacccgatc
    37021 actttctagt gagatgattg ccagtagtat cgaaacaaat gcagcaaatg tacgtaaaat
    37081 catgagtcag ctcaaaaaat ccggtttgat ctttacaaaa accggaaaac cgcagccttc
    37141 ccttgccaaa agtccagatc agatttcttt actagatgtc tatcgcagca tcgaaggtaa
    37201 tacacagctt atccaagtcg atccaaaaac aaatccaaat tgcgtcgttg gtgcgaatat
    37261 ccaagaagct ttagaagaaa gctacgctcg gctgcagaaa aaggcagaag aagaaatggc
    37321 taagatcaca cttgatagtc tgatacataa aatagccgtt ttggaaaaag aaaaaagacc
    37381 agaaaacaca ggcctcgtag agagattttt ataattctct ctactgtgcc tgcatttttc
    37441 tgatcggaat cgtctacata aattctttta ttctccgggg gacaaaagcc ttgtcatgcc
    37501 cctttttcct ctctaatgct caggtcaaag cgctgaagtc atgacgtctt gataaacggt
    37561 gttcaagtag ctgaccctcg gcattagttc tgaaaaatgc aaaatatgga aagctatttc
    37621 ccatttttcc tctctaatgc tcaggtcaaa gcgctacttt cacgacctct atattatcat
    37681 ggtttattga ctaccggtat ccagatttct cccatatatt tcccgttggt aactcccatt
    37741 tcgactacag catttggacc cccaacatag gcgtaatctt ctacttctct taaaacatgc
    37801 ccaaaggccg tattcgtcaa atcatttctt aattcttcag gcgattctgc taaagcgatt
    37861 acaacaagat acgttccttt tgggaatggg atgattcggg tagtgtttgg aacatcctga
    37921 ctcgtttgaa caccgacata gtacatcatt ttcccgttat agggttcgtt gactgtaaag
    37981 aaataatgat tttcagcagc ttgtttcaat gcatctattg ttccattacc gataactttt
    38041 tgctcaaatg cttgtttctc tgcagccaaa ccgacaaaat ccgtattgct actcttcaat
    38101 tccacaccaa tacccgtcac cgtaaaatct ggtttttctt cgatcctata ctttgccatt
    38161 tctcccaccc tttctattat attaagttca tcttacataa aattaaaggg aaatgacacg
    38221 attacgctct tttttctagg gatattttta ttttttaact atttttttgc tttcactgca
    38281 atccaaatct cactgtaaac attactgaaa tcctctcccc attttgtaaa agagatctcg
    38341 ggagcttcga ctaactcata atctgaagaa ggaagccatt ctgcatagat tttcccccaa
    38401 gtatcttgta aagtttgggg aaatggccct ttattaggaa aaatggccca agtgtgttct
    38461 tctatagcaa gcatttctaa atcagcgtac ggattttctt ttgtcgtagc aaagccaatc
    38521 atgtgcatca aactgccctt ttcttccaag cgggctccat caaatccata agaaacgttg
    38581 attacttgat ttggatataa atctcctaac ttatgcatct cttccctttg agtctccgtg
    38641 atcgtctgcg ctaacttttg tatttcttga ttgactcctt caaatttgac aggaactttt
    38701 ttcgccacac ctgccacata aaactgttct ttcttttcga tacgaaattc catcgatttt
    38761 cctcctctga catcaataaa aaatgtcaag cgtggaaaag atttttgtat gccgtatctg
    38821 ctgatttctg aaggcagata tcctgaccaa tcacgaaaag ctcttgaaaa accttcgact
    38881 gtttcatatc catatctaaa cgccgtttct gtcactcttg atccatctag gagatcgcga
    38941 ttagccaaag ccaatctacg gtttttgata tagtccatta gcgacatccc tgcaataaac
    39001 gtaaagatgc gttttaattg atattcagag attcctgtat tttcggctat tattttatag
    39061 gaaagtcctt ctgtcaaatg ttcttcgatg taatcgatca attggttcag atctttaagc
    39121 atatttatct tcctccttgc ttgccttttt atcatattat caaagaaagt caattacttg
    39181 atgaatcagg aaccaattta gtcagatgta gaaaatgttg tatggatcta aagaaaattc
    39241 tcaagcagcg tttcgtaacg gataattggt attatctgcc tatctatgtt ttacctttag
    39301 aaaaataaat tttggggaag acacattcta agtaaacaaa gcggtaaaaa tatcttttat
    39361 tcccttagtt aatccgctta ctacctactg tacaaagcaa attgttgttc aaactgctga
    39421 tcagaacgct gcttggattt ttctttgcga tacatcatac ctcttttatc aattggaaca
    39481 attatttatt tctaacaaaa tcacttcatt tttgtcagat aaataaaaaa caaaaaaagt
    39541 tccatcttta aaacacgcta ttcttttact ttttctcaca ttctctaa
//
LOCUS       DAXLPI010000014        36142 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXLPI010000014 DAXLPI010000000
VERSION     DAXLPI010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.014"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(89..937)
                     /locus_tag="ACF3T5_000626"
                     /pseudo
     CDS             complement(89..937)
                     /locus_tag="ACF3T5_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1090..2202
                     /locus_tag="ACF3T5_000627"
     CDS             1090..2202
                     /locus_tag="ACF3T5_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFD3631260.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2280..2573)
                     /locus_tag="ACF3T5_000628"
     CDS             complement(2280..2573)
                     /locus_tag="ACF3T5_000628"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFD3631261.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            2741..3055
                     /locus_tag="ACF3T5_000629"
     CDS             2741..3055
                     /locus_tag="ACF3T5_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFD3631262.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3076..5055
                     /locus_tag="ACF3T5_000630"
     CDS             3076..5055
                     /locus_tag="ACF3T5_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFD3631263.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5068..5394
                     /locus_tag="ACF3T5_000631"
     CDS             5068..5394
                     /locus_tag="ACF3T5_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFD3631264.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5410..5928
                     /locus_tag="ACF3T5_000632"
     CDS             5410..5928
                     /locus_tag="ACF3T5_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631265.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            5928..7280
                     /gene="celB"
                     /locus_tag="ACF3T5_000633"
     CDS             5928..7280
                     /gene="celB"
                     /locus_tag="ACF3T5_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFD3631266.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7364..8869)
                     /locus_tag="ACF3T5_000634"
     CDS             complement(7364..8869)
                     /locus_tag="ACF3T5_000634"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFD3631267.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(8961..9551)
                     /locus_tag="ACF3T5_000635"
     CDS             complement(8961..9551)
                     /locus_tag="ACF3T5_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFD3631268.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            9730..11376
                     /locus_tag="ACF3T5_000636"
     CDS             9730..11376
                     /locus_tag="ACF3T5_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFD3631269.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            11425..12144
                     /locus_tag="ACF3T5_000637"
     CDS             11425..12144
                     /locus_tag="ACF3T5_000637"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFD3631270.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            12623..13570
                     /locus_tag="ACF3T5_000638"
     CDS             12623..13570
                     /locus_tag="ACF3T5_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631271.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            13582..14502
                     /locus_tag="ACF3T5_000639"
     CDS             13582..14502
                     /locus_tag="ACF3T5_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3631272.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            14543..16000
                     /locus_tag="ACF3T5_000640"
     CDS             14543..16000
                     /locus_tag="ACF3T5_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3631273.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16345..16959
                     /locus_tag="ACF3T5_000641"
     CDS             16345..16959
                     /locus_tag="ACF3T5_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFD3631274.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            16952..18676
                     /locus_tag="ACF3T5_000642"
     CDS             16952..18676
                     /locus_tag="ACF3T5_000642"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD3631275.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            18673..20124
                     /locus_tag="ACF3T5_000643"
     CDS             18673..20124
                     /locus_tag="ACF3T5_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD3631276.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(20597..22750)
                     /locus_tag="ACF3T5_000644"
     CDS             complement(20597..22750)
                     /locus_tag="ACF3T5_000644"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFD3631277.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(22752..23798)
                     /locus_tag="ACF3T5_000645"
     CDS             complement(22752..23798)
                     /locus_tag="ACF3T5_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3631278.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            23948..25237
                     /locus_tag="ACF3T5_000646"
     CDS             23948..25237
                     /locus_tag="ACF3T5_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFD3631279.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            25250..27952
                     /locus_tag="ACF3T5_000647"
     CDS             25250..27952
                     /locus_tag="ACF3T5_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFD3631280.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            27937..28794
                     /locus_tag="ACF3T5_000648"
     CDS             27937..28794
                     /locus_tag="ACF3T5_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFD3631281.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(28865..29722)
                     /locus_tag="ACF3T5_000649"
     CDS             complement(28865..29722)
                     /locus_tag="ACF3T5_000649"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFD3631282.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            29933..32104
                     /locus_tag="ACF3T5_000650"
     CDS             29933..32104
                     /locus_tag="ACF3T5_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFD3631283.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            32172..32513
                     /locus_tag="ACF3T5_000651"
     CDS             32172..32513
                     /locus_tag="ACF3T5_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFD3631284.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(32789..34543)
                     /locus_tag="ACF3T5_000652"
     CDS             complement(32789..34543)
                     /locus_tag="ACF3T5_000652"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631285.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLSFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(34554..35390)
                     /locus_tag="ACF3T5_000653"
     CDS             complement(34554..35390)
                     /locus_tag="ACF3T5_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFD3631286.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(35392..36075)
                     /locus_tag="ACF3T5_000654"
     CDS             complement(35392..36075)
                     /locus_tag="ACF3T5_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFD3631287.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        11617 a         6363 c         7372 g        10790 t
ORIGIN      
        1 atgactcaag tttattgggt aatactttga ttttctttct acaaataatc agcgatcagt
       61 tgtaattttt tctctagttt taatggctct ttgtcaataa ggagaaaaac aagtcgggtc
      121 ttttgtctcg gaaagcttca accaactcgt gaataaaggt tacgcctcct tgaaaggcgt
      181 ggaaaaacca agtaatcgat caatcaccaa tttcgcattt ggtagaacat gtttggtgag
      241 ctgaaagtag gtggcgttca tgtctgtaac taagaattct atttctttag gattagtgca
      301 gctaaggaaa tagtttgtta atcgaggaat tttttgtgta ggtaaatttt ctcgattaat
      361 ttttctgtct cgccatccgc ggaaataaaa ctcagtttat cctcaacgga aacgtgagaa
      421 cgaaattcac aaccctcaac actcaagaca gaattctttt ggaagacttt ggtaaatagc
      481 ttctcaactc tttcaatgtg cgaataacgg ttgtcaaaga tacctaacaa cttttcgaaa
      541 taaaaaataa cgacactttt tcagtcagta aagaagtgat tttatgtctg acatgatttg
      601 aaatcgagtg ccttggacgg ataaaataac tttgagcgtt ccaatggctt cgacagtttt
      661 tacaggtata gcgctgcttt tttaggcgta taaccattgg catatgatta tattattcaa
      721 agtggacaat ggtttccttc ttcttgtttt tcaccaccat taatttccta tttccatcta
      781 ccacagcaga accacaattt ctacagacac atggagcagg gaagagaact gcagcaacga
      841 tcaatgtttt tttcttctgt aaggtttcgt aagagacctc tgtaatcatc aaatttttgt
      901 ctattgatct cagaattttt ttgatagaac cattcataaa gcatatcgtt ctctcagttg
      961 tttattttgt ggtgatttaa tcatacaaga aaacgatatg tttttttata aaaacatgaa
     1021 taggaaaaat agttgacaag aaaatgacaa cgccatatga tgttccatag tatttacaac
     1081 ctgtcataga tgactgtaaa aaataaaagt aaaaagaaag gacgacaaca ggtggatttt
     1141 tatcagtctc tacaactaga tccgtttatt ttaaaacaaa aaatacgaga agcagcttct
     1201 aaaaaagaaa agtgcatgta tatttgttca ttgtttttac gatcattatt catcgtattg
     1261 tttgccattt gttttatcat tataattaca acattatttg aatcgaagca taaaccatac
     1321 gctgtggttt tattctgtat gttgatgagt attcgttttg ttgactttgg ttataagatt
     1381 tcccattcga tcataggttt agcaatcgtc atgttctcac tattagtagc accgtatgtc
     1441 cagctgatca aatggtcagt tatgggcatg ttgattcatt ttattttact aagcagcata
     1501 ttaatggcaa ccgctagtga tcccaaaatg gggaatgctt ctttgtacgg cttttcatat
     1561 ctctttattg tttattcgtt acctaaagat tcgttgaaca agaatttctt tacacaaaca
     1621 agtagcttat tatttttgtt tttttgttgg ttttcagttc ttctttacag aaaacatcga
     1681 gaaaagaaca aagacaaaag cttgtttaaa gagatttttt tgaaaggcat gtattcgcag
     1741 gaaaaaattt ggatgctcat ctatgcattt ggcattagtc ttttgatcgt tgcaggagag
     1801 tatgtccctt ttcagcgatt gatgtgggca gggtttgcat tttcttctat tgtatctagc
     1861 tatggattga tgtcgattgg tttcaaagaa cgagcagtag accgaatcat tggctcattg
     1921 attggatgtg ttttatttat tggtatcagc caatttattc cttttaactg ggtcggtata
     1981 ttaggtggtc tagctttagg tgtttgttct acctatagat ataaaacagt ttttaattct
     2041 tttggtgctt tggcaatcac tgccagttta ttcggggttc caggagcagt aacgatccgc
     2101 atttttgaga atatccttgg ggtatgttta ggaatcatgt atattggggt gactgaaata
     2161 cttatccgaa aaataagaga aaaacacggt ttgaatcatt agatgagatc gaataaaaaa
     2221 taaggaaaaa gtgaagatac acatggatga taaattgcta tcattccatg tgtatctttt
     2281 tacggtaaat cagtgaaacg ttcgactttc atttttaatt tatacttttt acggagttcg
     2341 tttatttgag ccatcatttc ggatttgtga tgcttttcga ttgcttcttc atctttccaa
     2401 cggtcgatca gtaaaagaga ttctggatct tctagtggaa agtaatagtc atatttctcg
     2461 tttccttctt ctgcacgaat cgctttgaca atgtttcttt cgaccatttc ttctgcaaat
     2521 tttctggctt gtccatttgt acttgtatac aaaatattca ctgtgatact cactaaatcc
     2581 cctcctgaaa aaattctatc actagtatat cacggtgcca gaaattatta ttcaagatat
     2641 gatacacatt ttttccgcaa tgctacggaa atatatattt tgaatagaaa acgcttacaa
     2701 gatatgcttg gtttataaaa ataaaaggag tgattgattt atgaaaaaag cattgattat
     2761 ttgtgcggca ggaatgtcat cctcgatgat ggcctcaaag acaacggatt tctttaagca
     2821 aaaaagagaa gaggtactcg tagatgcagt ttcagctaca gaaggcgata atatgattaa
     2881 gaatagtgat tttgatttat ttctgatcag cccgcaaacg acgatgtttt tggataaatt
     2941 caaaaaatta ggagaatctg ttggaaagcc tgttgttagt atccctttcc aagcgtatgt
     3001 cccaatccct acaggcatcc agaagttagc agaggtgatc gaagagaaca tatgatggaa
     3061 taggagtgga tgataatgaa acagaaggaa aaattgctgt tgagatattt gattgatcac
     3121 aagaaggagt atgtcaccag tcaacgttta gcttctgaat tgttattatc tgatcggaca
     3181 atccgtaatt acttgcagcg gatcaaagag ttagtagaaa aaaacggagg taagatcatt
     3241 gctaaaccag gttatggcta ccaactgcat attttacaaa gactgacatt tgacttgttt
     3301 ttgagtcggc aagaaatagt acctggatat gctagagaac cgcaaggatt ttatgagtcc
     3361 gaagatcgac aaaagtatat attgaaccaa ttgcttttgg aagatgccat tctttttatg
     3421 gatgatttgg cagaagagct ttatatcagt cgttccagtt tgacaaagga catgcaagaa
     3481 atcaaggagc gattgattcc atattcacta aaaatcgttt ctaagcatgg tcaagggaca
     3541 tggatcgaag gggaagaaag aaatcgacgg cattttatca tggatacttt ttttggaaac
     3601 cattatggaa attcattgaa agaatattta gggcaaagcc gttttttccc agacatcagt
     3661 tttgaagagc tagtcattat cattctcgat gaaacaagag aagctaagtt aaaagtttcg
     3721 gattttatca tacagaatct gactttacac ttggctttgg ggatcaaacg gctccgagaa
     3781 gggtttgaaa tcaaggaatt aggcatcgaa caagaagttt ctaagcgagt agagtatcaa
     3841 gtggcgcagc ggatcgttca acgtattgag gtaatcgcag gtgtatcgtt tcccgaagaa
     3901 gaaattgctt atttgacatt acatctcatg gctaagtcga atcacaaaga aaatcaaaat
     3961 gatcaggaac tatcaaagga attagcgaaa gttctgaaaa gattgtctca ggaatttcat
     4021 ttttcattag tagaggatct ccagcttttc aatggactac tcgatcattt gtcaccgatg
     4081 atgattcgat tgagccgagg tattcgcatt gagaacccat tgactgagga gataaagaaa
     4141 gaaaatccca aagtctttga cgcagtaaaa aggcatttca gcaacatgcc ggctttgaaa
     4201 aactatacca tcaatgaaga tgaatgggcc tatttggctt tgcatctcat ggcggcattg
     4261 gaaaaagaaa gagctgcgca taaactacat gcattgatta tctgtgcaac gggttacggc
     4321 agtgcccaac tattaaagaa tcgcgtagtc agtgaattcg gtaaaaatat tacagtggta
     4381 agtgtaaaag gctattatga gatcaatgaa acaactttaa aaggaatcga cctgattatt
     4441 tcttctattg atctttcgac aatgtttttc aaaattccag ttttacatgt cagtattttt
     4501 ttgaatgaag atgatgttcg taaaatacga aaggtagttg gagaatctat accctatcat
     4561 tcctcagata gaccgatacc agcattttct ctacagcaaa aaaagcagat ttacaccgaa
     4621 cagttgtctg aaaaattttt caagacttat acgtacgaac cgaaaaagga agaaattctt
     4681 catgatttgc tgcaaaagct gagcataaat gaagaagaag gatatataag agaaatgcga
     4741 aagcagatcc agcaacgtga aaatatggga caaatcatat tcagtgatac agtggcagtc
     4801 ccacatcccg ctgttccagt tggaatcgaa acaaaggtag ccgttgcatt gattccttct
     4861 gggatgcagt gggatcaata tcaagaaatt cattttgttt ttttagtttc gccttcttat
     4921 gtcgaaaatg aaggaattac ggtcgtgact aaagcaatcg ttaggttagt tgaccagcta
     4981 aaagtacagc aggaaatctt agctgaacct atttttgaga atttcagcag taaatttatc
     5041 aaactgatcg aataaggagg aaaacagatg gctgaaaaaa taaacagtga aacactacaa
     5101 gtgacttctt ttgatatcat tttccatagc ggaaatgcac ggacactgat acatgaagca
     5161 tttcgctcga tgcgtaaaga gatgtttgaa gaagtagaag agaagttgcg acaagcaaat
     5221 gaagagattc ttcatgcgca tcagtcacaa actcaacttc tgaaagaata tgccagcggg
     5281 acaaaaatag aaatggaaat tatcatggtc catgcacaag atcatctgat gacgacgatg
     5341 acattattgg aagttgcaca ggaaatgaat tatttgtatc aaaaaaataa atagaaagga
     5401 aggatcattg tgcaggatac agtaaaaagg aaaatcgaat tagaaaaaaa gcaacttagt
     5461 ttgaaaaaca tgtatttcaa tcgttatcta atcgtacgtt acctgactgc ctttttcttt
     5521 ttcatgaaca tgcaatggat gattctgctt ctatcagcta aaagtttagg aagtagttta
     5581 ccgatgattc tgattttagc tattttgcct gcagtaggag aacaagtaaa gctttatcga
     5641 aaacaccaaa cgaacgttcc atggacaaag tggtattttt tgttccagag tgtctttaat
     5701 ttattagtga ttccagtgtt gtttacctca ggatttacat tcttgtatcc ttttatggca
     5761 aataacaatg gaggacagtt atttgttttc attcttgttg taagcggtat ttttgccagt
     5821 gcatttatac agtatcgttt aaagaaaatt tcactcaacc aagaccaaca gtatatccgt
     5881 attaaacaat atgaaaaagc gttatattta ggaaaggaga ataactaatg tttgattttt
     5941 tgcagaaata cctgatggga cctatgggga aaattgcgca acttaaaatc gttcgggctg
     6001 tcatggcagc ggggatggct tctattcctt tcacgattgt aggctcgatg tttttggttt
     6061 taaatatttt gccattaccg ttcccatttt tagaaggatt ttttaatgca acttttttca
     6121 aagttagtga cttgtacatg atcgtcaata caatgacgat ggggatttta tctatttact
     6181 ttgccattgt gtttgcttat gaattaacgt caattgaacg agatgaacaa aaattaaatg
     6241 ttaatccgct cacgggggca ttactatctg tttttgcatt ctttatgtgt atccctgaat
     6301 tgatcatgac tgatggtaaa atcagcttga ttgcttctat caatgagaca gaaactgtag
     6361 taaatggtat taggatgggc tcttttgtcg aacgtttagg aacttcagga atctttacag
     6421 ctattattat gtcgattatt gcggtcgaat tgtattgtat gtgtgtcaag agaaattggg
     6481 tcataaaaat gccagatgtt gttccgccag gagtttctcg ttctttcact gctctgatcc
     6541 caacatttgt cattgcgttt gtggtgatga tcatcaatgg attattagtt gcactaggaa
     6601 cggatatctt caaaatgatt gctatcccat ttgggtttgt tacgcagttg acgaatacat
     6661 ggcttggtgt catggtgatt tatttcttga tccatgcatt gtggatcgta gggatccatg
     6721 gagcgaatat catcacttct ttcttgaccc cgatcgtatt agctaatatg gcagctaacg
     6781 cacaaggcgc aaattatccg ttggcaggag aattcaacaa ttcttatgta accgttggtg
     6841 gatcaggtgc gactttagga ttgatcatct ttattgcatt cctcgcgaaa tctgatcagc
     6901 tgaaagtgtt aggaaaagcc tcacttgtac caggattatt caatatcaat gaaccaatca
     6961 ttttcgggat gccaatcgtc tacaatccct atttagcggt tccgtttttc ttagctccga
     7021 tggcttctgc tacactcgct tattttgcca ttcggctgga aatcgtcaaa ccgatgcttg
     7081 cacagatgcc gtggccttca ccagtcggga tcggagcatt tgttggtagt ggtggtgatt
     7141 ggaaagctgc tgtgttagca gtcatttgtg caatcatcgc attcattatc tggttcccat
     7201 ttatcaaatt ctatgataat aaattattaa atgaagaaag agaaaaggca gcagaattag
     7261 ctagtgaaaa gttaaaatga tttctaagaa aaaaggaact gtgacaaaaa atcacagtcc
     7321 ctttttttct tagaaatggg aaaccactag tatataaata ccattattct tcaatcaacc
     7381 gcttcacgat tgcataatcc ccttcataag gttcatctac accattgatt ttttgatagg
     7441 gatccacgcc ttttcccgca ataacgactg aatcttgcgg agcgctttct gctaaagcgc
     7501 gtcgaatcgc ttcaggacga tccatttcat aatgaacgat caaatctgga ttagtaattg
     7561 cttcattgat ttcttccgcg atttttttcg gatcttcaaa tgctgggtca tctgccgtca
     7621 aaaaggcgat gtcagctgtt tctgatagca cttttccaaa atcatgtcga cgggagattg
     7681 ctttgtttcc tgggctgcct aatacaacaa ttacacggcc atctggatgt tcatttttgg
     7741 caaattccag caatgttttc aagcttagat aattatgtgc atagtccaca tagacatgcg
     7801 ctccattgga ttggatcaat tgatccattc ggcctggtac acgagcttct tttaatccgt
     7861 cttgtgcatc ttgactggag gcacctgtta gtgctgcggc aatgatcgca ctggctgcat
     7921 tttcttggtt gaatcctcca gccagcaaaa tatcataagc agtatcgcag atagctaagc
     7981 gatcttcatg agaagataaa gtaaaaccgt gagtaccttt atctgaacgg atcacttgat
     8041 aattgctttc ttccgaacga ccataagtga tcgttggaat ctggaataga tcggctgttt
     8101 ccctaagtaa atcaaaatag tcactttcat gatttaaaac aactgttttt gaattcaaga
     8161 tcaattgtcg cttgcaatag aaatagtcat caaatgtcgg atgttcgatc ggactgatat
     8221 ggtcaggcga gatattcaag aagattccga catctaaagt caagccgtat acgcgttgtg
     8281 ttttgtaagc ttgtgaagaa acttccatca ctagatgagt cataccattt tcaactgctt
     8341 ctgacatcat acgatataaa tctaatgact caggcgttgt caaatgggat ttgaaatatg
     8401 ttttgccatc taacgttgta ttcatagtag aaagcaatgc tgttttttta tttgttgtgt
     8461 gatccaagat tgcttttgta aagtacgcag cagttgtttt accttttgtt ccagtgaatc
     8521 cgatcacttt caatttattt tgcggatagt cgtaaaaagc catagcaaga agcgccattg
     8581 ctttacggat atccgttacg atgattccta ctgctgaatt ttcgtatggt tgttctgata
     8641 cataatattg aataccctgt tcgattgctg aatccaaata ttcttctttg aaattcattc
     8701 ctttacagaa aaacaaagta gaagcatcag cttttcttga atcataactt aactgaagaa
     8761 attcagcttt ttctggtaca tcaagatgcc agcctcttgt tgaaacaaat tcttttaata
     8821 gattttcttt taatagtaaa tgacggatgt cttctaaaga gaagctcatt gattgatccc
     8881 tcgctttatt caatttcttg gtttattata gcacactctt tttaatatat cgtttgtttc
     8941 cgctatgaca aaatccagct ttattcaaat tttcttgaca caggggacaa gcggatgagt
     9001 attttcataa acaatgaact gactgtaata gataggagaa cgaatagtgt ctccaacaat
     9061 ccaaaaacat aataattcaa aaagacgata agactattgg taagtaaaag tccttgcggc
     9121 attggaagcc gaaaaaattt gtggaaaata atcggcggta tcgttatacc gccatttgag
     9181 gccccgactg tgtacaatgt gtaaacccca agactgaaca aaagacttcc tgaaatcagt
     9241 gaaaagaaat gtgaggagat catttccact tttggaacaa tctctaaaac aacaggaaaa
     9301 agcagacttc ccgctacagt cttaaaaaat actgattttc ctaataggac agcggctaga
     9361 agcagcatca taagattgat gctccaaaca gtaactgagc gttggataga aaaggccgtt
     9421 tcaactaaat gtccgatggc gcccactccg gcggaagcta tatcgtgagg gagcaagaac
     9481 caattcaatc caactgtgat aaagaaaaga cctaaaaata taacgggtag tttttttatt
     9541 tcttgtgtca ttgatatctc ctataaatgt tagccataaa aggctgtata tttctttact
     9601 ttaccaaaaa aacgaaaccg aattcaaaaa aacaattgtt cttataaaag ttatataaaa
     9661 tcgattgcca gtttctgttt taccaattaa aatgatagta atgttttgta gaaagttgga
     9721 atctgaccga tggataatca aagaaaacca gcattagatc aattaacacc gcaagaaaaa
     9781 atggcacgag gctctgcttg gatgaccgcc agtaatatca tttcaagatt gctaggcgct
     9841 gtctatatca ttccctggta tgcttggatg ggcgaaaatg ctaaggctgc caatggatta
     9901 ttcaatatgg gatacaatat atatgcattg tttctactga tctctacagc aggaatacct
     9961 gctgcgattg caaaacaaac tgcacgctat aattcattga atgaatatgg cacttcacga
    10021 cgattattta tccgtgcatt acagatgatg ggcgggctag gcctgttgtt tgctttgttc
    10081 atgtacatcg cctctccttg gctagctcat gcttctggtg gcggagaaga actgattcca
    10141 acgatgcgtt ctttgagtat cgctgtattg gtttttcctt gtatgagtgt gatacgaggg
    10201 tattttcaag gcaatcaaga aatgatgcct tatgcgctgt cgcagattgt agaacaggtg
    10261 gcacgtattt tttatatgct attggctaca ttcatcatca tgaaagtcat ggatggtgac
    10321 tatgtgacgg cggtcacgca atcaactttc gcggcattta tcggtatgct agcaagtatt
    10381 cttgtccttt tgtatttttt gaagaaacat caagcgtata catcggcgtt tattcactac
    10441 agcgaaaaca aaataaatat tgcaacgaag gaattgttgt tagataccgt taaagaagcg
    10501 attccgttta ttattgtggg ttcaggggta acgatcttca aattagtcga tcagttcact
    10561 tttatgaaga tcatgtctgc atccaccgag tattccaatg cacagttact ggatcttttt
    10621 tcgattttta gtgccaatcc agataagttg acgatggtag ttattgcatt ggcaacatcg
    10681 atcgcgtcta ctgggttacc gttgattaca gaagctgtta cgttgaaaga tcgagtaggt
    10741 ctggcaaaat tgacaagcgg aaatttacag ttgttttcat tttttatgtt tccggcgaca
    10801 tttggtgtta tgttacttgc ctatccgcta aatacattat tttatacacc ggattctcta
    10861 ggaagtaatg tactgatcca agcaagtttt gtaggactct tcctaggact atatatgcta
    10921 gtttccaaca tgctgcaagg aatgtttgaa aacaaggcgg caattaatta tttggttatt
    10981 gggtttctag tgaaactcct tcttcaatac ccagccattc gtttatttga agtatacggc
    11041 cctttattgg cgacaatgat cggatttgct gtgtcttgta ccttgatctt aaagagaatc
    11101 catgaagtag cccgctttaa accaagtttt gtctggcgca ggacattgct gatctttatt
    11161 cttaccttga ttatgctttt ggcttcgtgg atcacaaaga tgatttttgg tgctttttta
    11221 aacgaagata gcaaattcca gtctctattg ttgattcttg tagtagcagg tataggggct
    11281 ttagtgtatg gttatttagc tttgaaaatc cgcttggcag aaagactact aggaccagga
    11341 atagcgcgtt tacgacataa attaaaaata aaataagtga atcagaagag ctagattgat
    11401 gctcttttga aatgggggaa acgcatgcgt ttagataagt ttttagcaga tatgggactt
    11461 ggcagccgca aagaagtcaa aggattgatc aaaaaaggaa cgatcgaagt caatggacaa
    11521 gtggtgaaaa gcgataaagc gcaagtaaat gaattcgaag atgcggttac ttatttagga
    11581 gaaatcatta cgtatcaaaa agatttttac tacattttgc ataaacctgc aggagtcatt
    11641 tctgctactc aggataatca tgatcaaacc gtaatcgatt tattggaaga ccaggattat
    11701 cgggaagatc tttttccagt cggccgatta gataaagaca cagagggcct cttgatttta
    11761 acgaatgacg gggtgtttgc ccatcagtta ctatcaccta aaaaacatgt ggagaaagaa
    11821 tatctagctg aggttagcgg tataatgaca gaagaagata tccgtttatt tgcagaagga
    11881 ctgattattg atggcgatga gcagacattg ccggcaaaac tccaaattga tcaagtagat
    11941 caagagacag aaacttcaat tatccgtctg attttgcatg aaggaaagtt tcaccaagtt
    12001 aagcgaatgg tcaaagcagt aggaaaagaa gtcttatatt taaaaagaat ccgtatggga
    12061 aaattttggc tgcctaaaga tttgaataaa gggcaatatc gacccatgac caaagaagag
    12121 ctagaacaag tgagagaaaa ataattgaat ggatagctaa aagaataagc agagtagttg
    12181 tagcgccatc tgaaaagcag acttgtccgt ctgactttcg gatggctttt ttgtgaaatt
    12241 tcggcttatt tccgtagaag ctacttctgg aacaccgttt gttcggaata agggactgtg
    12301 acaagacttt tgttacagtc cccattgttc cttgtgactc agagttagat agtaaggaca
    12361 cgatctccct aatactcagg ccaaaacgct gaattcacaa cctctgataa acggtgttca
    12421 atcagctgac cctcggtatt agctcaaaaa acggcaaaat atgaggaact attttcccgt
    12481 ttttttctct aatactcagg ccaaaacgct gatttcacaa cctctcctat actttgtttc
    12541 attttcccta tactttgttt atttattctc attattgaaa gcgctataat tacgttgcga
    12601 atgaatcgaa aggggttttt agatgaaaaa acgaaagggg ttcaatcatt tttttatgaa
    12661 tctatggcgt tataaagcac ttgtcctgat ggctgtaccg ggtatgcttt ggatgatttt
    12721 tttcttttac atacctgtaa tggcgaatgt cgtagcattc aaaaattttc atatttcagc
    12781 agatgggttc ttggctagct tgagggaaag tccttgggtg ggttgggaaa atttcaaatt
    12841 tttattttct tcggatcaag catttctcat cactaaaaat acaattttat ataatgtagc
    12901 atttatcctt ttgaatctgc tgatctcagt cgtatttgca atcattatga gcgagttgag
    12961 aaataaacga ctcgttaaag tttatcaaac gatgtcctta ctaccttatt ttctatcttg
    13021 ggtcattatc agttactttg tttacgcttt tttaagtcct gacaaaggaa tattcaatca
    13081 gtggatcact ggacatggag gagaagctgt caattggtat aatgaaccga aatattggcc
    13141 gtttatcttg gtgttcattg gtacatggaa agggattggt tataacagca ttatttactt
    13201 tgcatctgtt atgggaatcg atccgactta ttatgaagct gctatggttg atggagctag
    13261 caaatggcag cagatcaaac atgtgacgat accacaactt attccactaa tgacgatttt
    13321 aacgatttta gccgtaggaa acattttccg agctgatttt ggcctgtttt ataacattcc
    13381 taggaattcc ggtgcattat atgatgtgac tcaagttttg gatacgtata tctacaatgg
    13441 cctgacatcc acaggagatt ttggtatgac ggctgctgca ggactttacc aatcagttgt
    13501 cggctttgtc ttgttgatga tcacaaatac gatcgcccga cgttttgata gcgattctgc
    13561 attattctaa ggaggaaaaa aatgaaaaaa aagaaagtgg agagagtcca agtccgctct
    13621 ttcaataaga cggctaattt tttctctaat ataatgattg cactctttgc tatttcctgt
    13681 attttgccat ttatatttgt tatcgccatc tcttttaccg atgagtccat attgtcgact
    13741 cacgggtaca gtttttggcc agaaaagttc agtatatttg gttatacgta cttgttcgac
    13801 cagatgcagg ataaaatttt ccaagcgctt ttcgtcactg tacttgttac tgttttaggt
    13861 acactgatca atagcacggc tacttcttta tatgcttatg cgatttcacg gtcaaacttt
    13921 ccattccgtc gtttttttac cgttttttgt ttgattacga tgttgttctc accagggatg
    13981 gtggccaatt atcttgttat gacaaatctt cttcagttaa aagatacgat ttgggcattg
    14041 atcttgccaa tggctgtcag tcccttcaat attattgtga tgcgcacatt ctttaaacgt
    14101 tcggtctctg attcgattat tgaatcggca cggatcgatg gagctagcga actgcgaata
    14161 tttgttcaga tcgttttacc gttagctgtt ccaggtattg cgacaatcag tttatttgcc
    14221 gcattgggtt actggaatga ctggttcaat gctttactat acatccaaga tgataagcta
    14281 gttccactac agtatctttt gatgaaaatc caaagtaata ttcaatatct gacacaaaat
    14341 tcaggagcag gaagccaatt gacaggcgga ttagcctcag ttccaggaga atctgccaga
    14401 atggcgatcg tagtcatctc gacgttgcct attgcgatca gctatccatt tttccagaag
    14461 cattttgtca aaggtctaac aatcggcgga gtaaaagaat aaagaaacaa aaatataaat
    14521 cataaaatta ggaggaagaa aaatgaagtt atggaagaaa ctagccttta caggtgtttc
    14581 agcgttgttg ttgggaacat tagcagcatg cggaggttct ggttctaaag accaggcaga
    14641 agctagcaat tcggacactt tgcagatgta tcaaatcgga gataaaccag ataatttcga
    14701 tcaattgatg gaagtagcaa acaaacgaat cgaaaaagag atcggtgtaa aagtcaatat
    14761 caattacatt ggctggggcg attacgaaaa gaaaatgaac gtcatcattt cttccgggga
    14821 aaattatgat atcgcctttg cgaacaacta tgtttccaat gcccaaaaag gagcctttgc
    14881 tgatttgact gaattagcgc ctaagtacgc tgaaaaagct tataatgatt tagatgaagc
    14941 atatattaaa ggaaatctgg tcaatggaaa attatatgct ttcccagtca atggaaatgt
    15001 ctttgctcaa caagtactta cttttaacaa agctttacta gataaatacg atctttcaat
    15061 tgatggaatc caatcttacg ctgatgcaga aaaagtcttg aaggaattcc atgaaaaaga
    15121 accaaacacg gcagcttttg ccattggaca aggcttcaaa gtccaaggga actttgatta
    15181 tcctttaggg aatacgttac catttgctat tgatctggat ggagatgatt cgaaaatcat
    15241 caaccaatac gataatgaaa cgtatatcgg actgttacgt acaatgcata gctggtacaa
    15301 acaaggactc attccaagtg acgctgcaac gagcaataag gattatccac tagaaggaaa
    15361 tacttggcta atgcgtggag aaacacaagg accgtatgat tatggcgata caattttgac
    15421 aaatgcagca aaacaagaac ttgtctcaaa agcaattact gttccactaa aatctacagc
    15481 tcaagcacaa atggcaaatt ttgtcgtatc aaatacttca aaaaataaag aaaaatcagt
    15541 agaacttcta gggctgctta acagtgatcc agaattattg aatgggcttg tttgggggat
    15601 cgagggagaa gcatgggaaa aagtcgatgg ttcagatgat aaaatcaaat tgctagatgg
    15661 ttatcagcca aatacacata tgtctgcttg gaatacagga aacaacaaga ttctatatac
    15721 acaagagtct attacggatg acatgattgc caaacgtgat caatcgattg ctgatgcaga
    15781 aacttcaccg attttaggat tcagcttcaa cactgacagt gtcaaaacag aattaagtaa
    15841 tatttctaat gtcatgaatc aatatttaga tggattgaat acagggactg ttgatccaga
    15901 tgaaacatta ccgaaattga aagacgcttt ggacaaggct ggctatgata aggtattgaa
    15961 agaaatgcaa aaacaatatg atgagttccg acaagaataa taatgaataa agacaaaggg
    16021 gttgtgacaa aagacgtgtc acaacctctt gttccgaata aacggtgttc aaaagccagt
    16081 ttctcggtat taagctaaat atatgtaaaa atgtggaaag ccatattcac aaaatttttc
    16141 ttactactcg aaactaagcg gctatttcac aaactcttgg taaacggtgt tcaatcagct
    16201 gaccctcggt attcgctcac aaaacgacaa aatatgagga actatttttc cgtttgtttc
    16261 tctaataatc aggtcaaggt gctgattgcc caaccttttt ttgtataatg agaaagtata
    16321 aacttaatgt ttggaggaga agatatgtta gggaaggttt tggaagagtt atttatccgg
    16381 atctgggtca ttataaaact aaccttgtat ttctggatat atacatttgc cggcgggatc
    16441 atctttggat taggtgcggc atggaaaaca gtcaatgaac tattttattt gtacggattc
    16501 gagtataaag aaatcacaat aaaaagaggc tggaatatat ataaaagaaa ttttttaaga
    16561 ggcaatctgc tatttagttt atttttatct ggtactgctt tattgagtta caatttatat
    16621 ttatctgttc aaatcaaagg gctcattttt ttagctattg attttatttt actttttgcc
    16681 ttgctctgcc tatttgctac ttatcaatac agtatgattt tagatagtga atatacgatc
    16741 tctgttccta atctgctaaa gctaagtttt atttcggttt tttccagctt ttcgtctttt
    16801 ctaaaaataa ttattggtag cggtataatt ttaggcgtga cttggcaatt caaaggattg
    16861 atcctgtttg gtgtcattgg tcttttgacg gtttggaatg gtacgatgac aacacattgg
    16921 cgtgaagaat tagataaaca gttggaaagt tatgaataag tttcggattc tattttcaaa
    16981 aggaacctta ctaaatcgtc tgttgcggaa atacgcggtt atcatgatcg gtgtaacgat
    17041 gactgctacc gttattttta gtatccatac ttgggaacaa aatcaaaaac aggcagaaaa
    17101 catgacaagt gatgccgttc aaagcacaag cagaatgctg aatgataaaa caaccttgag
    17161 taggatcatc aaaaaccagc ttgtagggaa ttccgaaaag atcgaaaatg tgacgaccta
    17221 cttaacaaaa cccatagatc agtatctgat gtatgtatac gaacagcaaa acagtaccga
    17281 tgaattagtt tctttcccaa atcaaatcaa agatctctac gcaaattatg aagaactaag
    17341 tgcgatatat atcgtgttga atcaactacc ggagtattat gaatcttcta gagaaaacaa
    17401 aggtggggaa ataaaaaagg ggacaccaaa attactagac gccttttata tcaagttgcc
    17461 tatcacacaa ggaggagctg agtcaggaag tatttttatt ggttttcaaa agaaagaact
    17521 agatatgatt ttagaaaatc taacgtcatt caatggactc tctttataca tgatttctgg
    17581 aacgacaaat cgactataca cattccatga taataaaata gctaaagaaa cgtttgaaag
    17641 acaagaaatg atcatcacag agagtctgaa gaaaaattca caattgccga ttgacgatct
    17701 ggaaaaaaac aattttgttc aacaccaaga actgtctgat gattatcgta tattagctgt
    17761 actagataaa aaaagtgtaa aaatcgaaac gggaaaaaat cttgccccgc tgatcattgg
    17821 tatcattatt ttagacaata tactgcttat ctttctttat aaaacattca aacgatattc
    17881 acagcaagta tctatcgtca tgcaggccat ggatcaaaca gcagcaggga atttagagac
    17941 gcggatcgac ataacaaaca cggaatatga actgaaggaa ctttcaatcg gtatcaacga
    18001 aatgttggat agcatcaatc agtttgtaga agacatctac aaattagaaa tcaaacagca
    18061 agacgcacat atgcgggcgc ttcaagcaca gatcaatcca cactttttat ataatacatt
    18121 agaatatatc cgaatgtatg caatcagcga aggaagtgaa gaattagcag atgttgtgta
    18181 tgcgttttcc gctttgcttc gaaacaatac gaatcaagag aaaacgatca ctttaaaaga
    18241 agaactggac ttttgcgaaa aatatgtcta tttgtatcaa atgcgttatc ctaatcgagt
    18301 agcctatcat tttatgatcg atcctgattt ggaaaagatc gaagtaccta aatttgtgat
    18361 ccagccatta gttgaaaatt attttaaaca cggtattgat tttactcgat tcgataatgc
    18421 gctaagtgtc aaagtcttgc aggaaggcaa acgggtaagg atcatcatca aagataatgg
    18481 aaaagggatg actgaaaaac ggctaaaaca gattgaagaa aaactgtctc atcctaaagt
    18541 ggaacttcac ggttctatcg gtctgcaaaa cgtgaatgaa cgtttacgtg ccagcttcgg
    18601 cagcagttat tatatgtcgc ttgagaataa tgagacaggt ggactgaccg tttctattac
    18661 attcaaggag ggatgagcgt gtatcaagtg ttattggtag atgatgaata catgattttg
    18721 aatgggttaa aaaagataat tgattggcaa agtttaggct ttcaaatagt agcaacagca
    18781 gagaatgcca aagaaggact agctgtactt gaacagcgac agattgattt agtagtaacg
    18841 gatgtgacaa tgccagaaat aaatggactg gaatttattg aagcggctca aaaagaacgt
    18901 cataattttg aatttatgat tttatcagga taccaagaat tcgattattt gaaaggaggt
    18961 atgcaattag gcgccgtgaa ttatttgatg aaacccgtga ataagttcga attggttgaa
    19021 agcctcaaga aaatcaaaac acggttagat cagcaaaatg aacaaaaaaa tcaacaagaa
    19081 atctatcaag aactgttatt cagtcaatgg ttgaatgaag aattggatga aataagtgaa
    19141 gaagaaataa taggacaaat aagtgagaag cagcatagga tagtattgat tcaactggca
    19201 agaactcatg atactcttat caatagatgg ttgcaagaac ggcaacaaat cttttactat
    19261 caacgaaatt acggagaccg tgtattgatc acgttgttgt tggaggcaga tgaagttgat
    19321 ggcttctgtt gttttgttca agaaaacttt cctgatcagg aatggctgat cagtatcggg
    19381 gaagagacag attctttgga caaaatccct gaaacattcc aacaagcgaa agacaatcta
    19441 cagttgcatc aattttatgg agataaagac gagcatgttt tttacgcagt acaagcagaa
    19501 acaaaagatc aaaccatcga tttttcaagt ttcagacgcg tgctgcaaaa taaacaattg
    19561 gaagtcgctc aaaaaatgat cactgatttt tttgaacagt ttcagttagc agccatgatg
    19621 ccggaagata tacgatattc cagtttttta ctgtttatgg agattcaacg ggaattgatt
    19681 gatctggaag atgaggaata tttacaagga atcgaaaaaa tccatcaggc aaaaacggtg
    19741 caagaacttc agcagctact tctgtcattc atcaaaaagc atcagagaca gaaaaagtac
    19801 agtgacaatg tggaaaaagt aatcgtaatc ctgcaccagc attatcaaga accacttaca
    19861 ctcaaagaag tgtcagaaaa cctgcattta aatgtcatgt atctaggaca gctgttcaaa
    19921 aaagaaacga aaaaaagttt ttcagcttat ttgaaccatt tacgtatgga aaaagcgaaa
    19981 caactgcttt tgcacagtaa tcaaaacatc aatgagatag caagtgagat tggctacaac
    20041 aacaccacgt atttctctaa attattcaag aaaatcgtcg gcagatcccc taaagagtac
    20101 cgagaaaaca gtggagaatc ataaaaaaaa aagccaatgt aatgaaataa cttataaaaa
    20161 attgtctaga aatctaattt tgggctgttc ttattacttg aggcaaggca gctttttcat
    20221 gacctctcga taaacggagt tcaaaagcca gtttctcggt attaagccaa atttatgcaa
    20281 aaatgtggaa agccattttc acaaaatttt tcttaatact cgaaactaaa cagctttttc
    20341 acgacctctc cactaaatgg tgttcaatca gctgaccctc ggtattagct caaaaaatgg
    20401 caaaatatga ggaactattt tgccatttgt ctctctaata ctcaggtcag aacgctgatt
    20461 tcacaacctc tcaataaacg gtgttcaaaa gcttgcatct gcggtaataa gaccaaccaa
    20521 acgaaaaata tgaggatcta tttttgcttg gctgttctta ttacttgatg caggacagct
    20581 ttttcacaac ctcttgttaa ttatagtttg ataaggaaaa aggcaaatct tttgcttgat
    20641 atttcttagg gtgagggatg atccctaatt ggtaagagat cgtgccgcct ttcagcaaat
    20701 cttgatgggt aaaatataat ttcgtatatt ctctgtccat ccgtttgatt ttatggataa
    20761 attgttgctg cggccgatta ggcgttgctt caatcgtcaa ttcctttcca tttgacaaaa
    20821 gcaaaacagc cttgtcaatt aatggcatac cgataacata ttcaccggtt ccaggcgtga
    20881 caggataaaa tcccagacta ttgaagatat accaagcggc catacttcca ttgtcctcat
    20941 ctccagggta acccattgga gaatcgtcga aggtctgagt catcaactgt ttaagcagtg
    21001 gctgtgccat ttcaggttgt cctatataac tgaacaagta aggatagtga aaacttggct
    21061 ggtttgaaat agctaattgt ccaaattcta tcgcggccat ctcactcatt tcgtggatct
    21121 caaaaccata tcctcctggg tcaaacgttg ggggctgatt gcacagctca atgagttttt
    21181 ctttatatct ctcactgcca ccatattttc cgatcagtcc tgaaaaatcg tgataaacag
    21241 caaagctgct ttgccaagca cttccttctg cataatcttg tccccaacga ttagctgaaa
    21301 atggttctct gaacttgcca tctttatctt ttgaacgcat aaatcctgac tcatagtcaa
    21361 agatattttg atagttcaat gattgctgct gatatttctt cgtcatttcc tcttcttcta
    21421 gtaccttggc aacttggcta atacaaaaat cgctataagc atagtctaat gtgtgattga
    21481 ctgattcgtg gtagtgatta ggaacatagc catatgtcaa gtaatcttct gttccttggc
    21541 gtccataatt tggatgtttg ctttgaaccg tagcaccttt ttccattgcc gttagaaatt
    21601 caggcatcaa gtctcgtcgg atatctttta cagcagcatc tgcaatgacc gcatcgatca
    21661 atgtcccagg cataagtcca cgttcatcag gagacagcca ttttggcaaa tagccacttt
    21721 ctctatagct gttcaaaaaa ccttccagca tttcttcgta tttttctaca gcaatcaaag
    21781 aataaagagg ataaaccgtt ttatacgtat cccaaaaccc attatttgta tagagaacac
    21841 caggttttat agatcgacta gtcgtatcat agtggatttt ttgcccacta ggatcaattt
    21901 cataaaatgt ttgtggaaat aaaaacaccc gatataaatt atgataaaac gtccgcaatt
    21961 gttgttgatc atggtgtgtg agctgtatac gatcaaaata ggactgccat tgttcttgac
    22021 tgtgagcaag gtaagtctct ttgctttggt tttgttctag ttccaggtta taagctgctt
    22081 gttccgagct gataaaagat gtcccaaatc ggatagtttg tttttttgtc tgttcaaaat
    22141 acaacgggat cgagtcgcta tttttgttgg aaacagctaa ctcatcaatt gttaaaggat
    22201 attcaaatct caatataaaa taaaaagaaa aatcaggatc ttctgaacca gcaaaatgtg
    22261 tgatttttcc ttccactgtg taaggatcta taacaaagag ctgatggcgt cccggaaagg
    22321 ataaaagtaa accgctgtca tttttgctgt aatcaataga taagattccg ccatacatgc
    22381 taggtatcaa tgtagaacag atgccataac gtaattgaca gatcgacaga tgagtcgggc
    22441 aaaatatact ttcttctggg cggtaagaac tttgagcatg gaaaagggtg ttctcctgta
    22501 gttttccatt gatcggcgta aacagcatat gactgaaatc acccatccaa ggacttggtt
    22561 gatgggtaag acggtagcct tgaaaaacgc ggtcatcagg atgaaaccac cagctgccgt
    22621 tttgatccgt agtttgcggt gcaaaataat tcatcccaaa aggaacgcca gtataaggct
    22681 ggcagttccc attagagaaa tttggctgat tcgctgttgc atgtctagta tctatttgac
    22741 cgatattcat tttatttctc ctttgttgat tgtcttttga tgaatgaaac tggaaattta
    22801 ctatcggatg gtcgatcttt tgtctctaac caagtgacca aagcatctcc cgcaagctta
    22861 cctaactgtt caaaatcttg tgcgatggtt gtcagtggcg ggtcaacaag agctgctgct
    22921 tggatatcat caaatccgat aattgaaaaa tcttcaggga tatgataacc atgctgtctc
    22981 aagagattca tggtctggat agcgacaaca tcgttttcac aaacaaaagc agttacttca
    23041 tacttagtga tataagaaag caattcatct aatgtcgcgc ttttgtcatc cagtgaagtg
    23101 tggaaagtca aaccagcttt gtccaatgct tcaaggtaac caaggtaacg ctgtcgaacg
    23161 gattgtggat gagtctgcgt accaaacaaa taagcgattt ttgaatgtcc ctgttcgatc
    23221 aaataagagg cagcaagcgc accgccttgg aaattatcgg aaagaacggt aggaaaaggt
    23281 aactcataga tttttttatc taatatgacg acagggaatt ctttcaatga aagctcaaaa
    23341 agtaagtcaa gatgttgttc tgtatcaaga gcataataaa tcaatccgtc aaattcattg
    23401 attagctctt ttgctgtttt attttgtaga aagtcaagcg tcgtcatcat gacatcaatc
    23461 tgtttatctt gcatcacagg gttcaatccc ttgctaaaat caccaacaga caaatcagcg
    23521 acatatggaa gaagaaacag aatccgtcgt gtttttgtag gagaagcaag aatctgttcc
    23581 ggagaagcgg atttaacgaa actgcctttc ccgcgaacgc gataaataag tcctaattgc
    23641 tctaattcag tcaatgcacg tttggaagtg attctgctta ctttataggt acgtgaaagt
    23701 tctttttcag ttggtagttg gctgtctaca ggtaattcac cagttaaaat agccgtttgc
    23761 aaatcattaa atattgtctg gtacaatggt tgagtcatat aaacacctct taatgatata
    23821 tcatattcaa ataataccat aatggatgaa aaagtaaata aaaagatatt ttttgaaagc
    23881 gtagacataa ttggctgtat gaagtataat ctttaaagat atatcaaata gaaagaaggt
    23941 tttattagtg gtttataaag atataccaaa atcggtgcag aaatttatgg aagatatcac
    24001 acaaaaatgt ggggatatcc atgcggactg ggcaaagaat tttacagcag catttgccaa
    24061 tactctgttg acaacagtca aaaagcaaaa tgacggaacc acctttttat tgacgggaga
    24121 cattccagcg atgtggttaa gagactctac tgcacaagtt cgtccttatt tagtgattgc
    24181 aaaagaagat gaggaattag ctgacatgat cagcgggtta gtcaaaaagc aattttttta
    24241 tatcaatatc gatccatatg cgaatgcctt caacgaagaa gctaacggtg ctggccacca
    24301 aacagatcat acaaaaatga acgattggat ctgggaaaga aaatatgaaa ttgattcttt
    24361 atgctaccca atacagttgg cctatcttct ttacaaaaat acaggaaaaa ctgatcaatt
    24421 caatcaatct tttattgaag gtatcaaaaa aatcttggac gtgttcaaaa cagagcaaga
    24481 tcatgaacac tctccttata catttgtacg agacacgaca agaaaagaag atacgctgat
    24541 caatgacggg cgaggtgcaa aaacagcttt tacaggaatg acatggtctg gtttccgtcc
    24601 tagcgatgat gcttgccgtt atggatatct ggtaccatcc aatatgtttg cggtggtcgt
    24661 gttaggttat gtggaagaaa tttttcaaac gatactcgat gataaagaaa ttgtacagca
    24721 aacacagaaa ttgaagaaag agatccaaga ggggattcag aatcacggac gaacggttaa
    24781 tcaaaaaggg caaacgattt atgcatatga agtagatgga atgggaaacg ccacagtcat
    24841 ggatgacagt aatgtgccca atctgatcgc cgcaccttac ttgggatatt gttctgaaca
    24901 agaggaagat tatctgcaaa caagaagaac attgttgagt aaagaaaatc catatttcta
    24961 tgaaggaaag tacgcaaaag gaattggatc ctcccatacg ccagaaaaat acgtttggcc
    25021 gattgcatta gctatggaag gaatgacaac gtcagacaaa tctgaaaaag agcggatatt
    25081 gaatttactt gtagcaactg acgcaggaac acaccttatg cacgaaggat tcgatgtgaa
    25141 caatcctgaa aattatacga gagaatggtt ttcttgggca aatatgatgt tttgcgaatt
    25201 agtcatggat tactttgata ttagagtaga aaagtaggag gtatcgtaaa tgaaaaagaa
    25261 agtatatatc atttctcata gccactggga tcgtgaatgg tacatggctt acgaacagca
    25321 tcacatgcga ctgattgaat tgatagacga tctactagag ctatttgaag ttgatccgtc
    25381 cttcaacagt tttcatttgg atgggcaaac gattatttta gatgactatc ttcaagtaag
    25441 gccagaaaaa agacaggcaa tccaacaagc aatcaatgaa ggaaaattga gaattggacc
    25501 attttatatc ttgcaagatg attttcttat cagctcggaa tcgaacgtga gaaatatgct
    25561 gattggcatg gaagaaagcc gtaaatgggg aacccctgtc atgttaggct attttcccga
    25621 tacatttgga aatatgggac agacgcctca attaatgaaa caagctggta tttcagcagc
    25681 agcgtttggc cgtggtgtaa aaccaattgg ctttgataat caagttttgg aagcagaaaa
    25741 ctattcttcc caatactcag agatgtggtg gaaaggccct gatcaaacag caatctttgg
    25801 tctgctattt gccaattggt atagcaacgg gaatgagatt ccagtagaaa aagaagcagc
    25861 tcttgccttt tggaagcaaa aattagcgga tgcagaacaa tatgcctcta cgaaccactt
    25921 attgatgatg aatggtgttg atcatcaacc ggtacaaaaa gatatatcaa aagcaatcca
    25981 tctcgcaaat gaactgtttc cagattacga gtttattcat tctaatttta ctgactatct
    26041 ggaagcagtt caaaaagatg tgccagaaga cttaggctca gtagaaggag aattgacaag
    26101 tcaagaaacg gatggctggt atactttagc caatacagca tctgctcggg tttacttaaa
    26161 acaatggaat accaaggtcc agcgacagct agaaaatatt acagaaccac ttgcgacgat
    26221 ggcttatgaa gttagtggaa actatcccca tgatcaatta gattatgctt ggaagacatt
    26281 aatgcaaaat catcctcacg acagtatttg cggttgttca gtcgattctg tccatcggga
    26341 aatgatacct cgttttgaaa aagcagatga agttggaaag tatcttgcac aagattcctt
    26401 agaacagctg accgcagcaa tcgatacgac cggattccca aaagacagct ttccatttgt
    26461 cattgtaaat actgcaggaa tggataaaac tggtgaagca gaaataacca tcgaattaga
    26521 aagaaaaaga ttcgctgaag gaatacctga acaactatat caagagttag aaaatttgcc
    26581 taaacgtaag tatcatgtag aaacaaaaag tggggccact ataccagcta ttttatctga
    26641 agaaactgtc caatttggtt atgatttgcc gaaagaccgt ttccgtgtgc catatatggc
    26701 aagaatgatc aaagtgactt taccattaga aaacatgcca gcattttctt gggaaacatt
    26761 cgcgcttgta gaaggggaag cgaaagcaga agaaaaggaa acaatgattc atcagagtgg
    26821 acgaatcatc gaaaacggcc ctctacattt gacgattgaa aaaaatggta ccattacaat
    26881 ggaagatcgc aaaaacaaac gaaagttgaa taatctgcat atttttgaag atatcggcga
    26941 tataggaaat gagtatatct ttaaacaacc tttctgtgat cagccaatcc tatccagtaa
    27001 caaagaaaac tctgaagtaa aagtattagt agatacaccg gaaatagcaa aaataagtat
    27061 tctacaggaa atggaaatcc ctgtttctgc agacgagcgt ttagaaaaag aacagcagat
    27121 ggttgtggaa tttcgttatc gaaaagcaga acgatccaaa gaaaaacgaa tccttcaaat
    27181 aaaaacaatc atgacaatca gaaaagatag taaaaaaatc gactttgaaa cgactctcga
    27241 caatcagatg aaagatcatc gcttacgtgt cttgtttcct acaaagttac atgtggaaca
    27301 tcacgaagca gatagtattt ttgaagtggt gaagcgccca aaccatgtgt ctaaaagttg
    27361 ggaaaacccc acgaatccgc aacatcagca agcatttgtc aatattcatg atgaagagta
    27421 cggtgtaaca gtcggaaact ttggtttgaa tgaatatgaa gtaacagaag acggacaaat
    27481 cgcagtgacg ctacttcgca gtgtgggaga actaggcgat tggggatatt ttccaacacc
    27541 agaagcacag tgtctaggtg agcatcggtt caattacagc attgaactac atggaccaga
    27601 agaaaaattc tctacttatt tgcatgcata cgcagcacag attccttttt ccactcaaca
    27661 aatcaaacat catgaaggaa cattgatttc aaaacaacaa tatttaacga taaaaagtga
    27721 aacgtttgca attacagctt tgaaacgcag caagtttagt gataaagtag tagtaagagg
    27781 tttcaatatg tctagtcatt tagaaaaatt ggaaataact aaagacaatg gaaaaactgt
    27841 catattgaat ctgcttgaag aaccaacgaa gcaagcggtt gtcccaataa tccaacctta
    27901 tgagattcgg acaatcggat tcgaggagga aaacgaatgt atggcggaat agaagcaggc
    27961 ggaacaaaat ttgtgtgtgc ggtttcagat ggtggagaaa ttttagaaaa attaagtatc
    28021 cctaccacaa caccagaaga aacattggat caggtatttg aattttttga tcgttatgaa
    28081 ttgaagggaa tcgggattgg ctcattcggc ccgattggta tcgacaaaac aaaagataat
    28141 tacggatacg ttttggcaac tcccaaaaaa gggtgggcac aattcgattt tcttggcaca
    28201 atcaaggaac gctacaatat tcctgctgct tggacaacgg atgtcaatgc tgctgcatat
    28261 ggggaattga agcaaggagc agcacaagga aaaaatagtt gtatttacct taccgtggga
    28321 acaggaatcg gtgcaggtgt cgtgatcaat gaagaaattt tttcagggat cgcacatcct
    28381 gaaatgggac atatttgggt gaaacgccat ccagatgacc attatgaagg tacttgtcct
    28441 tatcataaag attgtttaga aggattagca gcaggaccgt ctattgaggc acgcacaggg
    28501 atcaaagggc aagatctgcc gcaagatcat cctgtttggg ctattcaggc ttattatatt
    28561 gctcaagcac tgatcaacta tacgttgacg ttagcacctg aaaagattat tttaggcggt
    28621 ggggtaatga atcaggatca tctccttcaa aaaatccgtc agcaatttgt tgaattgatg
    28681 ggtggttata tggaaacacc tcaagtaagt gaatatatcg ttcgttgggg actgccgaat
    28741 gaaagtggga ttatcggtag tttgctgtta gcagaacacg aacatcaaaa gtaagcagac
    28801 aaattacaca aaaagaacag agtcttatca ttatctgctg ataagactct gttctttttt
    28861 atttctattc ccataatcca ggtaaagctt taccgactat tttttcttga gtaaatggat
    28921 ggatcatgtg cagttctaat gcatggagca tcagtcgttc agctgttcta ttttggtaga
    28981 gagggtctcc aacgatcgga tgtcccttat gagctaaatg aactctgatc tggtgtgtac
    29041 gtccggtttc caaaacacaa tagacttgtg tttgttcaga aagtactttg ttgacagtga
    29101 catgtgtcaa ggcgctctga ccatttttat catctacgac cctttttctc cgatcatggc
    29161 gatctcgtcc aattttattg gtgatggtca tatctttggc aatttctccc catacgatgg
    29221 cttgatatct ccgatagatc attttttgtt ccaacattct tccaagtatc ggcaagacaa
    29281 atggattttt tgcaaataaa acggcaccgc ttgtttcttt gtccaagcgg tggacaacgt
    29341 aaggtcgttg ctgtttagtg tcgagataag cagctaaatg attgagtaag gtactgtttt
    29401 ctgtcggttc atttggatga gtcttcattc ctgcaggctt atccactata atcaaatgct
    29461 catcttcata taaaacacgg atgtgcttcg catctcctaa ttgaacttct tgataattat
    29521 aatctgtttc ttcgaataac aatgtgatct ggtcattgcc ctttacttca tgatggaaca
    29581 aagcaggttg ttgattgatc caaacgtttt tacgtatacg caaaaagtgt cgaacctttc
    29641 taggaactaa ccattccttt tcaagcaagt cgcgaattgt tgtttcagga tggttcatag
    29701 gtaaagtgat cgtgattttc acaagtgtac tcctttcagt gcaattgcag actaattgta
    29761 ccatttttaa aacatattta agaaaaaact tacatttttc tgttcattct tatgttactc
    29821 tacttgcact aacgtaaaaa aacatattga actacaactt aagcaggaag aaaacactct
    29881 ttcactatgg taaaataaga cgattgaatc aaatagacag gagaagaagc aaatggattt
    29941 tcaaagcatt ttagaaaaga taaaagctgc tttgatcagt ttttggcgtt ggatcagacc
    30001 atatctcgga caattccatc gatggcgaaa gcgcatctgg aaaaaatatc atgtgaacaa
    30061 attgattttg ctgattggtt tgatctgtgt cttagtgaca agtatctacc tctttatttt
    30121 agcaaaacaa gcaaatgtcg aaacgttaaa atcaggatta agccaatcga cagtcattta
    30181 tgataagaaa aacgaagaag ctggcacact gtacgcccaa aaaggaacat atgtagaatt
    30241 aaacgcgatt tctccctata tccaagatgc agtaatctcg acagaagata ggaattttta
    30301 cgagcatcac gggtttgata tcaaagggat tgcccgtgca gcagtccgaa tggtcttgtc
    30361 tggcggaaca tcagggggcg gaggaagtac gatcactcag cagcttgcca aaaatgcgta
    30421 tctgacatta gaccaaacgt ttgatcgaaa agcgaaggaa ttatttttgg caatcgagat
    30481 cgaaaagaag tacagcaaag aagaaatctt gaccatgtat cttaacaatg cctattttgg
    30541 caacggggta tggggtgtcc aagacgcttc cagaaagtat tttggagtag atgcaacgaa
    30601 tgttacgctg tctgaggctg ctactttggc aggaatgctg aaaggtcctg gtatttataa
    30661 tccgatcgat cacccaaaaa atgcgaatga tcggaaaaac acagtactaa gtgtaatgga
    30721 agaaaacgga aaaatcacaa aagaacaaga acaagcagaa agccagacag atatcagcgc
    30781 gtatcttaat gacacatacg ataattcaga ttctggttat cgttatcctt attattttga
    30841 tgcagtgatc gatgaagcag ttaacgagta tggtcttgat gaagaagatg tcatgaataa
    30901 aggctataaa atctatacag ctttagatca ggattatcaa aagcaattag aagctactta
    30961 tcaaaacagt gcattattcc cagcaaatgc ttcagatggt gccatggtcc aatctgcttc
    31021 agtagcttta gatccgaata caggtggtgt acaagcacta gtaggacgaa gaggagatca
    31081 tgtctttaga ggattcagct ttgctacaca gatgcgtcgt tcaccaggat caacgatcaa
    31141 accattaagc gtatatacac cagcactgga agctggatac aagccttcca gtatcttgaa
    31201 agacgaaccg caaagttatt acgatgcgca taattttgac gggacatacc aaggagaagt
    31261 accgatgtat caagctgttg cacaaagttt gaatttgcct gctgtatggc ttttgcatga
    31321 gatcggttta caaaaaggat atgataaagc agaagaattc ggcctgcctt tagcaaaatc
    31381 tgataagtat tatggcttag cccttggtgg actggaaaaa ggagtatctc cattgatcat
    31441 ggcgggtgct tacagtgcat ttgccaatga tggtcagatg tatacgccgc atcttatcac
    31501 gaaaatcgtt gattcgactg gagcggtgat cgtcgataac acaaacaaaa aaccaaaaca
    31561 ggtcatatca aaggaaacag ccgatgaaat gaccagcatg cttcttggta cattttcaaa
    31621 tggaacagcg gctgctgcga atcctgcagg ttatacgata gcagggaaaa caggaaccac
    31681 agaaactaat ttcgatgcca caaaagcaaa tgaccaatgg atgatcggtt acacgccaga
    31741 cgtagtgatt tcgacatgga tgggttacga agaatccagc aagctgcact atttggaagg
    31801 gacaagtgga aatgttgtcg ggaaagtctt caaatcagcg gcagaaggca tccttccgta
    31861 tacttcaaaa acaggattta gtgtagcaga tgcctatgcc acaggaggac aagtggtagc
    31921 tgcagatcaa gttggaaaca caggaaattc taatggagag tctggaaatt ggcaagataa
    31981 cttgaatcaa tatggtgaac aagcaaaaga agggctgaaa aacttcggcg atatggtgaa
    32041 agaaggcgtt cagggaattg gtgaagcggc taaagacctc tggcggaaat atcaaggaga
    32101 gtagcccctt tgaaaacgaa taaccccatg ttacaataga gagtaactac taaaagaaaa
    32161 gagggcataa catgtcaaac atctacgata gcgcgaatca aatcgaacgt gagatccgtg
    32221 aattaccaga attcaaagcg ttgaaagaag cgtacgagaa agtacaagca aacgaagaag
    32281 cacacaaact gttcaaagac ttccaagcga tgcaaatcga attacaagaa aaacaaatga
    32341 gtggacaaga gttcagcgac gaagatgcaa tgaaagcaca agaaatggct gtgaaaatcc
    32401 aatcagaaga agtaatcaat gacttgatgc aaaaagagca aggcttcagt acaatcatca
    32461 atgacttgaa ccgtatcatc atgacaccag ttcgcgacct ttacagcgaa taaaactatt
    32521 ttatgaaaca acagagggtg tgaaagagac gttcggcctt gagcgatgac aaaattgctt
    32581 cttattgccg ataggctgtc tcttgaacac catttaccaa gaggtcgtga gcctaccgtt
    32641 taactctgag ttacaaggaa caatttcaaa aaacagcttc tcatgtttga tgactattgt
    32701 gacttgtgac cgaggagttg gcaggagaac accgtttatt cagaaatagg agctacgaca
    32761 aaagtcttgt catagctcct attttttgtc attcattttt caagcggtag aaatagcctt
    32821 tttgagctaa cagtttttcg taagtaccta tttcaacgat ttttccttca tcgatcacaa
    32881 cgattttatc tgcctctttg atcgttgaaa gccgatgagc cacaataaaa gtagtgggtg
    32941 tttctgtcag gtaattcagg gcttgctgga tttttttctc tgactggtta tctaaagctg
    33001 aggttgcttc atctagcaaa atgatttttg gctgacgtat caacgctcgt gcaatggaga
    33061 tacattgctt ttggccacca gacagcttgt tgccatgttc gcctactaag gtatccagac
    33121 catcaggaag ctggtcaatc agtgaagaaa gttgcgcagc ttcgatgact tcatctaaac
    33181 gttcttttga tacgtttttc aagccataag tgatgttctc tttgatcgaa gcggagaaca
    33241 acaaagtggt ttgaggaaca acggctaaat aatttcgcac acttcgcata tccagttcat
    33301 ccaaaggctg atcatctaga aaaatttttc ctgaagtcgg ctggataaat ccaatcaata
    33361 agttaaggag tgtagatttt ccagagccag atggcccaac aaaagcaatt gtttctccag
    33421 ctttcacctc tagagaaaaa tgatcgataa tcggtttgag tgagtccgga taaagaaaag
    33481 aaacatcctc aaaacgaaaa tttccatcca aatgttggat ttttttctta ccgtgataag
    33541 tttctacatc gccagcaatc aacagttcgc caatagaagt gatagattcc atgccttttg
    33601 aaatcgtagg aatcagcgtg atcattccag aaacggaatt gacgatcgtc gtgaaatacg
    33661 tctgatacat cacgacatcg ccgggttgga tgcgcccttg taaagctaat atcccagtga
    33721 aactcaaaca aatcagctga aatatctgaa aactagccca gctgactgat ccgaaaacag
    33781 attgcaggat atctaagtgg aatccttttt catagatttg agtaaaatgc ttatttaagc
    33841 gagttgtttc gtgtttttct aacccatgag ctcgagcaat cggtgctaat tcgatcactt
    33901 cgatcacttt tgcagaagtc tcttccattt cttgcctgaa gtctgagttg gactgtctga
    33961 tccttttacg gaaaaagaga gtaacgaatg cggcgagagg aacagtcaat aagaaaaaca
    34021 tgaaaacaac aggactttta taaagcgtga tacctaaagc tacgaataaa ttgacagcga
    34081 tatttaatcc gctgacaaat acttgagagg acaacgtttg gatagcttct acatcacgga
    34141 tgatctttga ctggattcgt ccagattgca attctttttg ataaggaata gatagttctt
    34201 ggattttctt gataagggtc gctcttagtc cagcttctac ttgacggatc gaagtgctat
    34261 ggtattttac atgaaaataa ttcgtcaata tattttgacc aatcagacaa gccatcaaaa
    34321 aagcgttgaa ataaatggcc tgcaactgag aagaatcttt cgatgaagct gcattgatta
    34381 cattggcaat agcgatcggc attaaccaag atggaagatg tttgataata aaaaagagtc
    34441 ctgacatggc aaaacgccaa taatttccct gataaagatg cagcaatgtc tgaaaagcat
    34501 ttgcatgata tttttgataa cttttctgta ttcgttcctc cataatcttc tccttaacaa
    34561 tcttggtaaa taaactgcaa gtgttctatt gcttcaggaa cataaatctc aggagtagct
    34621 tcaagagtgg cgtacattag aggacgctca tatttgatat aatgcagtac cgggcggaaa
    34681 tctaaacagc ccttgccaac aggtaccatg cggatctttc cattttccat gatatagtct
    34741 ttgagatgaa gcgctgtaat ataaccatgt aaattgtcca aggcacctcg gatcactttt
    34801 tcctgatcag catgattttc tttatgcatc aaattcgcac aatctaaaat gatttttaaa
    34861 tttggtgaag ctacttcatc gattaaacgt ttagctaact gatagttata gattggatga
    34921 ttgatccctg cttcaacagc aacagttacg ccaaaatttt ctgcatgagc gactaattca
    34981 atgatagaat ttctggcgat ctgataagct tcttcagtaa aattttcttt tgtgtaaccg
    35041 acagagacac taccggtttc cgtagcaatc atcttggctt gataggcaga gcacagagca
    35101 atttgctgtt tgaaagttgc caatgcttgt gcacgaactt cttgattttt gctggatagg
    35161 ttgatatagc atcctaaaat ggaaagttcg atattgtttt tttggaagta ttcaccaaaa
    35221 taggcagcac tcccaagtgt catgtcagtt aatttaagtt gttcaggaaa tgctttttgt
    35281 ggtgcaaatt ggatatggga caagtgaagc tgtgatactt tttgaacgat ttcttcacga
    35341 tttcgaacat ctaaatcgtg ggcacggatg cctaaattta atggaaacat ctcaaactcc
    35401 tttttgtaaa taattagcaa gaggcagacg ggattctttt atcaattcaa caattattcg
    35461 ggcaacttta tcagcgcctt ctggactaaa gtgggtattg tcttttagtc cttctgggta
    35521 atttttacaa gtatcaggca taagatgcag gtagaatttt tttgtctctt cttttgagaa
    35581 ggtctggaac aattcttgtg atcttgagaa cacatctaaa agaggaatat ggtgtttctc
    35641 agcaaatgca cgcatcgctt ttggatagtc tccaagtata tctggattca atgttccgtt
    35701 ttctaagtaa tctcgtcgcg tcacagatgt cagtaagacc atttggacgt tcgctttttt
    35761 tgcgcattca aaaaatatct gcagattttt taaatagcta ccgaagggaa gcgtacctct
    35821 ttcttcttgg attttctggt cgttatgtcc aaattgtaca aataagaaat cattcggctg
    35881 aaactgtgaa gctagacggg ctaaccttcc ttcctggata aatgatttgg tgctgcgtcc
    35941 attctttgct tggttattga tctggatctc gggtgaaaaa taagcagaga ttttttctcc
    36001 ccaacctgtc tcgggacgct tgttttcatc tttttctgcc gcagtagaat caccggcaat
    36061 atggatagta ggcatgttat ctcttctttc ataaaggttt ataggcaatg aagcagaaca
    36121 gcaagagaag aagacggagc aa
//
LOCUS       DAXLPI010000015        34447 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXLPI010000015 DAXLPI010000000
VERSION     DAXLPI010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.015"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(178..2070)
                     /locus_tag="ACF3T5_000655"
     CDS             complement(178..2070)
                     /locus_tag="ACF3T5_000655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631288.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2067..3794)
                     /locus_tag="ACF3T5_000656"
     CDS             complement(2067..3794)
                     /locus_tag="ACF3T5_000656"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631289.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3810..4460)
                     /locus_tag="ACF3T5_000657"
     CDS             complement(3810..4460)
                     /locus_tag="ACF3T5_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFD3631290.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4664..4960
                     /locus_tag="ACF3T5_000658"
     CDS             4664..4960
                     /locus_tag="ACF3T5_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFD3631291.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5132..5752
                     /locus_tag="ACF3T5_000659"
     CDS             5132..5752
                     /locus_tag="ACF3T5_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFD3631292.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5844..7010)
                     /locus_tag="ACF3T5_000660"
     CDS             complement(5844..7010)
                     /locus_tag="ACF3T5_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFD3631293.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7022..7375)
                     /locus_tag="ACF3T5_000661"
     CDS             complement(7022..7375)
                     /locus_tag="ACF3T5_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFD3631294.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7503..8525
                     /locus_tag="ACF3T5_000662"
     CDS             7503..8525
                     /locus_tag="ACF3T5_000662"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFD3631295.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8544..8705
                     /locus_tag="ACF3T5_000663"
     CDS             8544..8705
                     /locus_tag="ACF3T5_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631296.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(8964..9398)
                     /locus_tag="ACF3T5_000664"
     CDS             complement(8964..9398)
                     /locus_tag="ACF3T5_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631297.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9508..11367)
                     /locus_tag="ACF3T5_000665"
     CDS             complement(9508..11367)
                     /locus_tag="ACF3T5_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFD3631298.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            11368..11508
                     /locus_tag="ACF3T5_000666"
     CDS             11368..11508
                     /locus_tag="ACF3T5_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFD3631299.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            complement(11485..12645)
                     /locus_tag="ACF3T5_000667"
     CDS             complement(11485..12645)
                     /locus_tag="ACF3T5_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD3631300.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12952..14376)
                     /gene="araA"
                     /locus_tag="ACF3T5_000668"
     CDS             complement(12952..14376)
                     /gene="araA"
                     /locus_tag="ACF3T5_000668"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFD3631301.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14392..15090)
                     /locus_tag="ACF3T5_000669"
     CDS             complement(14392..15090)
                     /locus_tag="ACF3T5_000669"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFD3631302.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15047..16708)
                     /locus_tag="ACF3T5_000670"
     CDS             complement(15047..16708)
                     /locus_tag="ACF3T5_000670"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFD3631303.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16926..18326
                     /locus_tag="ACF3T5_000671"
     CDS             16926..18326
                     /locus_tag="ACF3T5_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFD3631304.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18578..19657)
                     /locus_tag="ACF3T5_000672"
     CDS             complement(18578..19657)
                     /locus_tag="ACF3T5_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3631305.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19743..20702)
                     /locus_tag="ACF3T5_000673"
     CDS             complement(19743..20702)
                     /locus_tag="ACF3T5_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFD3631306.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20714..22225)
                     /locus_tag="ACF3T5_000674"
     CDS             complement(20714..22225)
                     /locus_tag="ACF3T5_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFD3631307.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22247..22810)
                     /locus_tag="ACF3T5_000675"
     CDS             complement(22247..22810)
                     /locus_tag="ACF3T5_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFD3631308.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22816..23679)
                     /locus_tag="ACF3T5_000676"
     CDS             complement(22816..23679)
                     /locus_tag="ACF3T5_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3631309.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23693..24589)
                     /locus_tag="ACF3T5_000677"
     CDS             complement(23693..24589)
                     /locus_tag="ACF3T5_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3631310.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24764..26053)
                     /locus_tag="ACF3T5_000678"
     CDS             complement(24764..26053)
                     /locus_tag="ACF3T5_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3631311.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26025..27542)
                     /locus_tag="ACF3T5_000679"
     CDS             complement(26025..27542)
                     /locus_tag="ACF3T5_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFD3631312.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27740..28630
                     /locus_tag="ACF3T5_000680"
     CDS             27740..28630
                     /locus_tag="ACF3T5_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFD3631313.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="ACF3T5_000681"
     CDS             complement(28703..29527)
                     /gene="nadE"
                     /locus_tag="ACF3T5_000681"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFD3631314.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29539..31011)
                     /locus_tag="ACF3T5_000682"
     CDS             complement(29539..31011)
                     /locus_tag="ACF3T5_000682"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFD3631315.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31648..32010)
                     /locus_tag="ACF3T5_000683"
     CDS             complement(31648..32010)
                     /locus_tag="ACF3T5_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFD3631316.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32233..32985
                     /locus_tag="ACF3T5_000684"
     CDS             32233..32985
                     /locus_tag="ACF3T5_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD3631317.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33125..34048)
                     /locus_tag="ACF3T5_000685"
     CDS             complement(33125..34048)
                     /locus_tag="ACF3T5_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631318.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34063..34335)
                     /locus_tag="ACF3T5_000686"
     CDS             complement(34063..34335)
                     /locus_tag="ACF3T5_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631319.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10164 a         7187 c         5930 g        11166 t
ORIGIN      
        1 aaatcagcgg tttgacctga gtattagaga tacaaaacgg aaaaatagct tctcatattt
       61 tgccgttttt tgagctaata ccgaaggaca gctgattgaa caccgtttat tcggaaaaag
      121 ggactgtgac acgacttttg tcacagcccc taaaaaaaat aataaatttg tactattcta
      181 tgaagcttct tcttcagaaa actgactgtt ataaagatct gcatagaatc cgtctttttc
      241 catcaatgta tcgtggtttc ctgtttctac gatcgaacca tgattcatca caataatgtt
      301 gtctgcatcg cgtattgtag acaagcggtg agcaacaacg aaacttgtcc ggttttccaa
      361 taggcggttc atcgcttttt gaatcagtat ttctgttcgt gtatctacac tagatgttgc
      421 ttcatcaagg atcaggacat ctggatttgc taagaatgcc cgcgcaattg tgattagctg
      481 acgctgccct tgcgaaatat tgcttgcatc ctcgtttaat acagtgttat atccttctgg
      541 caatttacga acaaaatcgt ctacgtgagc agctttagca gcctcaatga tttgttcgtc
      601 tgttgcatcg tcgttcccat atttgatatt gtcatagata cttcctgtaa acagccaagt
      661 atcttgcagt accattgaaa actgtgcgcg caattcatca cgtgaaagat cacgggtatc
      721 tacaccatcg taacgaatac tacctccaga aacatcgtag aaccgttcaa gaaggttgat
      781 cagtgtcgtc ttccctgcac cagtcggacc tacaatagcg accatctcac caggtttgac
      841 atccaagtta aaatcagtca ttagcaagtt atctggtgtg tagccgaatt gaacattttc
      901 aaaacgaact ttgtatggtg tatctttttc ttctggaagg ccagattttt cattggacat
      961 ctcttcttca tccaacactt caaatacccg ttcggctgaa gcaactgttg attggatagt
     1021 gttcatcaaa cttgcaagtt gagtgattgg ttgagagaac tgtgttgtat attgcaagaa
     1081 cgcttgaaca tcccctaatg tcatgttccc atttgcaact tttactccgc ctagtacagc
     1141 gacgaacaca tagcctagat ttttaacgaa attcatcaac ggcatgatga ttgccgaaat
     1201 aaattgagct ttccaaccag cggcatacag attctgattt tctttttcaa atacttcctg
     1261 gtctttttca gtatgattga atgttttaac aatggtatga ccaccatatg tttcttctac
     1321 ttggttattc aataaaccta aacttttttg ctgatctgcg aaaaacactt gtgatttcgg
     1381 cgctacgatc atcacgatga tcaagcttaa agggatcatc aataacgcga ctaacgtcaa
     1441 ttgccagctg attgaaagca tcatcacgat gaccccaatc aatgtgactg cactagttac
     1501 aaattgagtc aaattttgct gcagcgtgct tgcgatgtta tccatatcat tgattgctcg
     1561 agacatgata tccccatttg tatgtgtatc ataataggaa atcggtacac gattcatttt
     1621 ggcttccaaa tcatgacgta attcataaac tgttcgttgg gaaacacgtg tcataataaa
     1681 ttgctgtaaa aagttgaata cagcagaaat caaatacatc acgattactg tcgcaatgat
     1741 ctgcgcgatt ttatcaaagt ctatcggaaa ttttgttact tgttggcctt gttgcatctg
     1801 ttgagcgcca gccattacac ctttgaatat ttctgtggtt gctttcccca agacctttgg
     1861 tgtttggatt tggaagatca cggctgctat tgctagtaca aatacagcga tgatcgcaac
     1921 catacgttta gacatatagc caaacaatcg gcgtaccgtt ccccagaaat ttttaggctt
     1981 ttcacctttt acaccgagat ttctgccagg tccaccatgt cctggaccgc ctgctggtcc
     2041 atgagaggga cgtgtgtttg ttttactcat gctaagtcct cctctcttag ttgtgaatga
     2101 acgatttctt gataagtttc gtttgttgct aatagttctt catgtgttcc tttgccaacc
     2161 agttttcctt catctagcac aaggatcata tctgcatcca ttactgtact tactcgttga
     2221 gcaactaaaa cggtgatact ttctttcata ttcgttttca atgcttggcg aagattggca
     2281 tccgttttga aatccaacgc tgagaaggaa tcatcaaaca catagatatc tgctgttttg
     2341 actaatgccc gtgcaattgc cagacgctgt ctttgtccac cagagaagtt tcctcctcct
     2401 tgttccacat ggctatctaa gccatcttcc atctctgaaa caaaatcttt agcttgtgcg
     2461 atttctaatg ctttccaaat catttcatct gttgcatccg gcttaccata ctgcatattg
     2521 tctcggatcg ttccagagaa cagaactgct ttttgaggaa caaagcccat taattctcgc
     2581 aaattatatt gagtcatgtc actaatgtcc gttccattta tttggatcga accagattcg
     2641 atgtcatata atcgagggat caaattgact aaggtcgttt tacctgaacc agtcccccct
     2701 atgattgcga cgatctcgcc cgacttggct tggaaatcaa tatcttccaa tgcaagtttt
     2761 tctgctccat gatatcgata attgacatga ttgaaggcca atgttgcgtt ttttccagca
     2821 aaagaaactg ttttaggatt ttcagggtca tggataccga ttttttcatc caacacttca
     2881 ttaatacggt cagctgaagc ttgtgcacga ggcaccatga taaaaatagc ggaaagcatc
     2941 ataaagctga tcaagatctg catcgcataa gtcataaatg caatcagatt accgacttcc
     3001 aaagtcatat ctgctatgta gtgtccacca aaccaagtga ttgctacgtt tgtaccgctc
     3061 atgatcaatg tcatcattgg catcatcaat gcgacgatcg tattaacttt gattgccgta
     3121 tttgtataat ctttattcgc taaatcaaaa cgattttctt caaagcgtgt cttatcgaat
     3181 gctcgaatca ctctgacccc tgtcaatcct tctcgaaata ccaggtttaa acggtcagtt
     3241 ttcttttgca tacttttgaa taatggaaca gcaaagtaca tgattccgcc gatcaaaata
     3301 atcattactg ggatgacaac taagaagatt tttgtcaact gatgatcttt ttgataagca
     3361 agtatgctcg cgccaatcaa cgtgattggt gcattgatca tcatacgtaa aaacatttgt
     3421 gtaaccattt ggatttgagt cacatcattg gtcgttcttg tgatcaaaga ggccgttcca
     3481 tatttatcaa acgcatcttt tgtcagactc tctgattttt tatagacatc cgtacgcaat
     3541 tgcttaccca gtttctgtga ttcacgagta gcaaaaaatg tatttgcgat agcagctaag
     3601 atactgatca aagaaaagcc gatcatcaca aaacctgtat gccaaatata atcgacatct
     3661 ccttgcgcta cccctttatc aataatattg gaggtaagtg tcggcaaata taaatctgcc
     3721 agtacttgga tcaccatgaa aactgcagct gcaattgctg aagtcaagga gatccgtttt
     3781 acgattttta gcatctagtt cccttctttc tatttatata ctccgttttt cagccaagaa
     3841 agtttcaaat tgaattcact gattgcttgc tctactgagt aatcttcaga tgttgaaaat
     3901 aagcgatacg aatgcgcaat tgttgtaaat acagtattca tcactaattg gatcagtaat
     3961 tccaattcat gatcattttt tactttcagc ttcgtccgat cgatcaattc aagcaactct
     4021 tttgcatctt gtttgtgttc tttatgtgga tcgtgaggcg ttttttggcg actcgtatga
     4081 ggagcgactt ttcttgaggc acggtaatcc atgttcataa acaagttacg ataaaaagaa
     4141 gcattctcgc cttccaaaat ctcataaatc attttagaaa aataggattc gaatcctttg
     4201 aatagatcac catcggattc tttcattagt tgtaacatat ctttagagct gtcacgtctc
     4261 attgtctcaa agtaataaaa ataaagatcc tctttatctt caaaatactg atagaaactg
     4321 cctctaggga tatccgctaa tttgacgata ttagcaatcg aagcttcttt taagggtact
     4381 ctagaaaact cgattctcgc agcctcgatt agtctttttt gtttttcttt aggcaaatgg
     4441 aaaaatgtct gtgtcggcat atggatcctc ctttctttca agatgacaca ttgtcatatg
     4501 acgctgtgtc attataacac ctcctacctg ttttgcaaga catttgcata gtttttttca
     4561 ttatcatccg atacattaaa aaagtgataa gaggaatcct tccatttgaa ttaatgagag
     4621 ttgcccatta aaatagccac atactaaaca aaggatgaga taaatggccc aatcaattag
     4681 tgtccaagac ttcaataaat tgcctctcga tgctgacagc atcgttttag atacaagaaa
     4741 taccttagat tacacaaaaa accacttaag ccactcgctt tctatccccg ctgctatctt
     4801 gccaagacaa ttaaaaaatc tagatccaaa gaaactttat tatattctaa gctatacagg
     4861 caggcgatca gaagcattag ccgatcaact tactgctaat ggttttcgtg ctgtttatat
     4921 cattggtggg atggaagctc ttcagtcgag tgttgcttga atattgcttg ttagattcaa
     4981 aagaggctgt gacaaaaaat cgtgtcacag cctcttttat accctgtctt tttttagtta
     5041 tttattcttc aaccttatca atatgacttg atagcctttt ctttttctca tagttataac
     5101 aatcatagca ataaaaggag aaggtaagca tatggcatct gttttagtag tcaaaggtca
     5161 tcccctaact gcagaagaat cgcgcacagt taaggcattg acgtcttttt taacaagtta
     5221 taaagaaaat catccagatg atgaagtgac tgttttggaa ctttatcgcg atgatattcc
     5281 ggaaatcgat gaagaattat tatcaggttg ggaagcatta cgtgctggtg cagaattcac
     5341 ttccttatca gaaagtcaac aacaaaaaat tgctcgtttc aatgaattga ctgatcagtt
     5401 ccttgctgct gataaaatcg ttatcgcaaa cgccttatgg aatctaaata tcccaacaaa
     5461 attaaaagct tggttcgata ccgtcaatgt agctggaaaa acgttccgct atacagaaaa
     5521 cgggccagaa ggattagtga caggtaaaaa agcactgcac atccaatcta atggcggtgt
     5581 atacaacgga caagactttg cctctcaata tgtaaaaggt attttaaact ttgtaggcat
     5641 cgatcaagta gatcagctat tcatcgaagg aatcgattat gaccctgacc gtgcagatga
     5701 gttgatgcaa aatgcattag ataaagctgc tgctttaggt aaatcatttt aaaaattgag
     5761 ataaatgcat acaaaagaga cagaggaaaa aatcttcaaa gcagcatttg taccttggat
     5821 attttcccat ctgtctcttt ttgttattct ttctgctttt tcaacggatt ttcttcataa
     5881 agagaagaga ttgttgtgta ccattcaaaa acatgagtaa tcactacttt agctgctgca
     5941 taaacaggaa tccccaagat cacaccgatg attccgaaca atttcccaga agtcagcaga
     6001 actaataaaa tcgttactgg atggattgct aactgattcc ctagtactaa tggagagatc
     6061 aagcgtcctt ctattgtctg ctctactgca aaaacgatca gtactttgat gatcatgaat
     6121 ggacctgcta caatggctaa aaagatagcc ggaatcatcg ctaaaaatga tcctaagtaa
     6181 gggatcaggt tcaaaaaacc cgcaataatc cccaacgtga cagcatactc taaaccaatg
     6241 accgcaaaac caatcataaa catgattgcc acagcaaaag ccacagttag ttgtcctctt
     6301 atataagaag aaacttgact attcatttca ctcagcacat tcaacgtcgg tttttgcatg
     6361 cgtgtcggta aaaatttgac aaaataaggt gctaattgtt taccgtcttt caatagataa
     6421 aataagataa acggcatcgt cagcagagcc actaaaatag tcgctaccgc gccgacaaaa
     6481 cttccaagac tctgaactgt actcttagat atatcttgga tcatatcgcc tgccgaattc
     6541 atgaacttat cacctgctgt ttccaactgt tcgcgaaatt ggttgaacag cgggtcccgc
     6601 aaaatctcgg ttaatttatt atcaatcgta tctacatatt gagggaagtt actgatgaag
     6661 ctcactgttt gttcctgaat cttagggatg atcactacgc tcccccatgc gattagtgca
     6721 acaaccaaaa caaacagccc aatgatactg taaagccttg gtacttttcg cttttcaaag
     6781 taatcaacta ccggattcat caagtaatac aagattcctg ctaaaatgat aggcagacct
     6841 acgattgcta aaaattgcca gattggtgca aacaaatatg aaacttttgt aaataaaaag
     6901 acgatcagca atattaacaa tacaacaagt aaggctgtga ctacttgatt atttaaaaac
     6961 cagcgccaaa accacgatac cggtctgttc ttcttatttt ttccttccat aattcctcct
     7021 gttagttata aaaaatttgg cttcccaaac caatagcagg cagtctatgc ggatgaagat
     7081 agattccatt tgcaatttgt ccttcttctg gacaagcatc aaaaatcaat gatacgtgag
     7141 ctactgtctc taaattccca ttggccatcg gaccgacata atcgatcgta tatacctgat
     7201 catcaaaagc aattgttccg cctgctttca aagaaaaagg ttcgtctttt gatacttctt
     7261 gaatcaccga atattttcgt aaggcagctg ttgctgtttt atcgaacaaa atcagcatgg
     7321 gttcttcctt gtttattgct tctgtcccaa tttctgtaat ctttgcctga atcattgcac
     7381 tctctccttt aattgctttc aaaaacagta taacacattc tcattcttta gccgtgtgga
     7441 ataattatgt gatagaataa aaacgaaaac tatttcatta caattaggag gaatcagctc
     7501 atatgatcga aaagataatt ttaggaacct atacgcgtcg cgaaagtaaa ggaatctata
     7561 cgatcgacct tgatacagaa aaagaagaat tagccaacct ttcattagct gttgaagaaa
     7621 atagccctac ctatgtagca aaaagtaaag ctggcaatct ttacacagtt actagtgtgg
     7681 atggattagg tggtgccggt gcgtacgata aggattttca ttttttgaat gcggtaacag
     7741 agtctggcgc tcctttgtgt tatgtagcgg ttgatgaagc acgtcaatta ctatacgggg
     7801 cgaactatca taaaggtgaa gtaaatgtct accatatatt aggagatggc gaactgaaag
     7861 cagcagattc aatattccat caagaagcga caggtcctca taaaaaccaa gatcatgcgc
     7921 atgtccacta tacggatctg actcctgacc aacgattagt cgtatgtgat cttggtacag
     7981 atcgtgtgta tacttatgat gtgtccgaaa acggcaaatt gaatcttgta actaccttca
     8041 ctgcagagcc aggaacaggc cctcgccatc ttgtctttca tccaaatgga caattcgctt
     8101 atttatttgg tgaattagac agcacagtcc gcgtgctttc ttataataaa gaaacaggtg
     8161 cttttgaaga attacagaaa atatcgacct tgccagaaga attcgacggt gaaaatggag
     8221 gggcagctat ccgcatttca aatgatggca agttccttta tgcttctaac cgaggacata
     8281 actcgattgc tgtattcgca gtttttgaag atggttccca cattcaaaac aagcaaatca
     8341 tctcaacaga aggggatttt ccccgcgatt tcgcactaga tccaagcaat aattttgtcg
     8401 tgtgtgccaa tcagaacacc gataatctga cattatatcg ccgtcatcct gaaacaggat
     8461 tactggaatt actgaaaaaa gatgtctatg taccagaatg tgtctgtgtt tattttgatc
     8521 gttagtaaga aaaggagaat gaaatgaatc agcaagacgt attagacaga ctacgggaag
     8581 aactaaatat tcctttcttt gatgggaaaa tcgaagataa agagtatagc gaagaggaat
     8641 atcagaaact caaagccgat ttacagaact actttgaaca atatgtacag aatgtagaaa
     8701 actaatcagc tttccatact atctgaaaag aacatcagga caagactttt ccgtctatcc
     8761 tgatgttctt ttctacaaat aaaaaaccgc cgagatggtt agactcgacg gaaaaggagt
     8821 atttactcga ttttgagtaa gtacaaggtt gttgttggtt ataggtatgt cattatcata
     8881 aaggattttg atcactctat cataaaggta atattacatt tatattagat aactctagtt
     8941 tttattgtta tcaaagattt cttttatttt ttcaacaaca aattagtcat tttttggatg
     9001 gcagctgtca attggtctgt cgcttggtct tgttttgctt cagatgtatt gaatagatct
     9061 tcatctaccc ctgattcaag atcgatactt tggatcattt cttcacagcc tgctacatat
     9121 gaaagataag ctttttcaaa atttttatga atgcccattg cttttgctgg tggacgcaaa
     9181 gtactgactt tcttaagcat tgcccgatat ttttttgttc cttcttcaaa taacaggatg
     9241 atctcttcaa gagtcgattt gtccagttca cttgtttttc cttcatcaat tgcgtttctg
     9301 atcacttcat atttcggatg cattccttct ccgattgctt ctgtttcctt gacgatttga
     9361 tcgatggact gcacataata gccaatattc ggtaacatgt tcgttttcct actttctcca
     9421 ttgttgttat tatttattct acttgttttt atcctcaaat gacaagtact tgaaaaaatt
     9481 taatggtttc tatacagctc gccattctta ttcgtgctca aagaaaccat ctgctaaact
     9541 atattgtttc cctgcgacaa tgtcatattg gatcaattga tattcgtcgt aaatctcttg
     9601 ttgcgagcga ttgaattgcg cttctttata ccactgtccg ttttgatagt acagttctcg
     9661 ttcaaacgcc ggaagttcct gttctaatga taaaagcagc tgataaaagc catttgttgt
     9721 ctgattcgtc tgttccatta gattcgctgc aaaataaaac ggactggtga ttgcatcgtg
     9781 agtcgtttgc ttttctaatt ctcctttact gtcgaacata agaaattgtg tttcatgcaa
     9841 tgtttgtttg tcattttttg cttggatcgt atctgaatag atccctggaa gatgatcccc
     9901 ccaaaaaaca actaaagtcc ttctagaaag attcttcaat tcttccgtga actgcttcag
     9961 tgccgtgctg ctataggaaa tatcttgcag ataattctct aagtcaagtg tcccacttcc
    10021 atctgaagtt tttgcaggat agtctagatt atcatacttt ccatcgtagg gcatatgtgt
    10081 ctgcatcgtg actaaatgaa tgaactgagg tgttttttct tccttgagaa gtgtcattac
    10141 ttctttgtaa gctgactcat cagaaatata tggattattc tctatcgtat ccgtataggt
    10201 catcgtacgc tcgctgatga actggtcaaa tcctaatgtc tgataaacat cctctctttt
    10261 atacatcgaa gtattgtatg gatggatcgc tgtcgtctgg tatgattgtg cgtttaacgc
    10321 tgaaacgata gaaggaagct gatccatttt tggtacaagc atcgtgtaag gggtggtgag
    10381 ttgcgcatta aataacgcca tagaaaaacc agtcaatgct tcaaattcga tatttgctgt
    10441 tccaccgcca tagttttgag aaagcatatt gccactatat gtttgatcag ctacttcata
    10501 ataatctgcc aatggctcac cagatacttc tacgccgttt aatcgactag gatctgagaa
    10561 actttcactc atcacgaaca cgatgttcgg cgactcttcc tctacagttt tatttttttc
    10621 atcggctagc ttctggtatt tttcagtgat ttctttgatc ttttctttcg aatatccttc
    10681 aggttcatcc attggttcta ccttcagatt gtacaaaaac ccaccgataa aaccagtgtt
    10741 atagtagttc attttctggc tgtaaggtat ccatagtgcc gttttattat atgcttttcg
    10801 caacaaatta tcgggattgt tgaaattact gatataccct agcagtccaa ttgccacaag
    10861 cagtgtcgtt aatcggatca attggaatcg ccttcctttt ttcaggcttc gaaacatgta
    10921 ccagcaaaac aatcctatca cggttcctgc ggcagctaag atcactgtaa acagcatagt
    10981 gccggtcata tctttcagca atccgatttc agtgatcatc ttcaaatcgt ctggataaat
    11041 cggttcttgc cgataactca ttttcatata atttgcaaaa cctaatacac cgatggctac
    11101 gacatacgta agggaaccta aagggacaga tcctgctagt gcaatcagaa aaataagaat
    11161 aatcagcaag acgagacagg ctaagaagaa tttttccgta tgccaagaaa aagcaaattt
    11221 caacgcaagg tctacggata gatcattttg acaccactgc aaaaataaat tactaaaaac
    11281 aacaagaaaa aagatactga tgattgctgc tgttcctttt aaaatctttt tctgtttggt
    11341 cgaaagtctg cttatatatc ttttcaaatg cttgctcctt catttctatc aataaaagaa
    11401 tgttgcttct cttattttag ttgtacgaaa ttcgaaagta aagaacggaa agaagaatta
    11461 atgaaatcca aatattctct ttctttatgg cctttctggt ttctttaata agcggacctt
    11521 tcgtactcca gaaatttttt ctagctgttg tttcacttga ttgagctgtt caaaatcagt
    11581 actgtctatg tcaatcaata tgtagacatg ctcattctta tggttacgcg atatatctgc
    11641 gatcgctagc tcgttctcat tcagcagaga aaatatctct gctaatatat tggttttctt
    11701 ttgataaaat atagtgaatc gaaacggcgc ttggaaaaac attcgtgcgg atggataatt
    11761 gaccgcttct ctaaccgtac cgaacaaaag ataatctttt aaggcttcaa ctgccaaacg
    11821 tccgctgtct gccaaagctt cctgagtcga tccccctaga tgaggcaaca taagtatacg
    11881 gtcattttct aagaactctt cttctggaaa atcagtcaag tatttagcca gatgttgttt
    11941 ttccaatgct tgcataacag catatttatc aacaatttca cttcttccta cattgataag
    12001 tacagcatct tttttcattt tttctatatt cgcctgatcg atcaatcctt ttgtatcttc
    12061 tgtcaacggc agcatgacta cgatgtaatc cgaggtagaa agaactgttt ctaaatctgc
    12121 ttgccgaaca taatccaact gggcatcgcg gatagaataa cccaagacat ccatccctaa
    12181 gctatagcaa cttagcgcta cttttgtgcc tatcgctcca agaccaagaa gaccgatggt
    12241 tttgccttgc aattctcgtc ccacataagc acttcttttg ttttctgcct gttctaaaat
    12301 attaggacct gttagtgttt gtaccatacg acttgcttcg attatcggac gggaagataa
    12361 gagcaaacag cacaaaacaa gctcttttac tgcattagca tttacacctg gtgtattcat
    12421 gacgatcgtc ccgttttcag aagctttttc aacgttgatc gtgttcaccc caacaccagc
    12481 tcttgaaatt gctagcaaat ctggtgtaag ccattcatct ttcactttcg tactacgaat
    12541 aactattcct tcggctggca aatcatctat tattttgaaa tattcccctt gtaacttttc
    12601 tatctcttgt tctggaatag catcaatcaa atgaacgtga tacataaaac cccctcctta
    12661 ctaacaagta tctcgaaatt tctgcaatta gacaactgtt taccatttga cttcctagaa
    12721 gcaaaaagag cagttagagc agagtaggct gagtgataaa aataaggcta tgacaagtta
    12781 ctgccataag ccttattttc gagagcaaaa tattgagggt gagatatctt gataaatggt
    12841 gttccgttag ctgacattga taataagact aaatttcaaa aaagttgaaa agcaaatttc
    12901 gtcaatctct tcttatttct taaaactaag tggctttttc acaacctctt tttatctaat
    12961 aaccactgtt tccaactcaa ctaattttgc ccacgtttca atttgatctg ttgttagatt
    13021 tagcgagaca acggtatgat gaccgccacc tgcctgaatc catgaatgga caccttcatg
    13081 gaaattcggt ttagttttcc ataacacacg tgcaacaggt aaattaggtg cttcagtcgt
    13141 tggttcaaag gcttctactt cattgatcag cagtttataa tgcgtaccga aatctgccat
    13201 cgatacgact accccttctc ctgctttccc atcaaataca agacgcgccg gatcttctct
    13261 atctcccatt gataaaggag aaacaacgat tcttggtttc gtcgcagcaa gcgtcggatc
    13321 tacttccatc atatgggatt ccaagattgc ttcttggcca ctagctagct cgtaagtata
    13381 atcttccatg aatcctgtat ctttccctcg agccatgatt ttcagcaacc gatcaatcgc
    13441 tgcagttttc cagtcgcctt ccccagcaaa accgtatcct tctgccatca aacgttgtac
    13501 agctagtcca ggtagttgtt tcattccata caggtcttca aaattagtcg taaaagcgtt
    13561 atagcctcct tcttctaaga aacggcgaat accgatttcc tgctgagctt gtacttttac
    13621 atgttcctcc catgtctttt cttcataatc accatagtcg aaggtataca attccttgta
    13681 ctcttcaaat aattgatcga cttcttcgtc tgatacgcga tcgattactt gtacaagatc
    13741 tccgatacca aaataatcaa cagtccaacc aaattgaatc tgtgcttcca ctttgtctcc
    13801 ttctgtgaca cccacgtttc tcatgttgtc accaaaacga gccactttta ttccaaaact
    13861 ttcattatag gctactgcaa catccatcca atcagcaatc tctttttgga tctcaggtcg
    13921 tttccagtag ccaacgacga ttttattttg tttatttaat cgagcattga tgaacccata
    13981 ttcgcggtca ccatgagcgg attgatttaa attcatgaaa tccatatcga tcgttttcca
    14041 aggaatgctt tcgttatatt gcgtagctaa atgaagcaat ggtttttgta gaagctttgt
    14101 cccgcggatc cacatttttg ctggtgagaa agtatgcatc caagtgatca cacctgctac
    14161 ttcttctcga taattgacct ctttcatgat ttttgtgatc gtatcagcag taactgctaa
    14221 ttcttgaagg cggattggat aaggcaattg gccattttca ttcagttcat cgaccatttc
    14281 ttgtgcatgt ttctttactt ctcgcagtgc ttcttcccca tataaatgct gtgatcctac
    14341 aacaaaccaa aattcttttt ctccaatatt caacataatt agccactcct tttatttgtt
    14401 ttgtccgtaa tacgcatttt ttccgtgttt tcgtaaataa tgtttatcta aaatccgttg
    14461 aggcagctct tctgaaaatg aattgagctg tctggtaaat aaattcatct tgcatacttc
    14521 atctagtacg actgcgttca ttaccgcact ttgcgcatct ttcccccaag taaatggacc
    14581 gtgtccgtgt aaaagtaccc ctggaattgc taagggatca attttgcgtt ctttgaatgt
    14641 ttctatgata acttttcctg tttcatattc gtacccgctg tcgatttcct gttgtgtcaa
    14701 aaatcgagca caaggtaccg atccataaaa agtatctgca tgcgtcgtcc ccatcgcagg
    14761 aacatccaat cctgcttgtg cccagatcgt cgcccatgtc gagtgagtat ggacgattcc
    14821 tccgatttga ggaaatgaac gatataaaac agcatgagta ggcgtatccg aagagggatt
    14881 gagtttacct tcaatgatat gattgtccaa gtccacaacg accatgtcgt ctgctgtcat
    14941 ctcttcataa ctgactccgc taggtttgat aacgaaatat cgtgtttcag gatcaaaagc
    15001 gctcacattt ccccatgtat atttgacgag tccgtgttta ggcaagtcaa gatttgcttg
    15061 gtatacttct tctttcagtt gttccagcat tttctatcac tcctttttct tgcaattgat
    15121 gaatcgctgt tttttcaatg tcgatcccct cacggtaacg tctcataaat atctgatagc
    15181 ctgctatttc ttctgctgaa ggtgtgatgg tgattccttg gtcttgtcca aacacgtctt
    15241 ctgccaagaa gttttctaaa gacttcccat gtcgatccat catataggct gctaataacg
    15301 cgattcccca cgctccacct tctccggctg tgtccatcac tgtaactggt gcctccatag
    15361 cagatgctag aatccgctgt ccaacttctg gtgttttaaa aatcccccca tgccccacta
    15421 aacgatcaat ttggacattc tcagatttca atatatccat tccgattctc attgcaccaa
    15481 aagcactggc taaatggatc cgcatgaaat tcgctaaatc aaaacggcta cctggtgtac
    15541 gtacaaataa tggccttcct tcatttactc ctgtaatgtt ttcaccggaa aaataaccat
    15601 aagaaagtaa tcccccgcaa tccgtatcgc cttctagcgc tttgttgaat aataccgtga
    15661 ataatttatc tctgcttatc tccataccca aagaattggt aaattcttca aaaatactga
    15721 cccaagcatt gatatccgaa gagcagttat tcgtatgaac catgcctact agttctcctg
    15781 aaggtgtagt gaccatgtcg atttccggat ggacattttc tagttctttt tctaatacga
    15841 tcatagcgaa ggcggaagta ccagccgaaa tgttccctgt ccgtttggct acactgttcg
    15901 tagcgaccat accggttcct gcatcccctt ctggcggaca aaccgggatt cctggtctca
    15961 aatttcccgt agggtcgatc agataagccc ctgattcgct taacttgcca gcttcttctc
    16021 ctgctactag aactttaggc aaaatgtgat ccaagtcaat cgctacgtgc gctttttcga
    16081 ttaactgacc aaaaatattt ttcatttctt gatggtaggt tttcgttgaa ggatcgatag
    16141 gaaacatgcc tgatgcatct cctacgccta gtactttctc accagtaagc tgccaatgga
    16201 cataacctgc aagcgttgtc aaaaaattaa tttctttcac atgttcttct ttattcaaaa
    16261 tagattgata caaatgagcg atactccatc tctgcggaat attgtattga aatttttccg
    16321 ttaatattcg ttctgcttct tcagtgattg tatttcgcca agtacgaaat ggtactagta
    16381 actgttcctt gctatcaaat gctagataac cgtgcatcat tgcactgaac ccaatcgacc
    16441 cgattgtcgt aagattctcg ccatagattt cgaatacttc tgctgccatt tttcggtaac
    16501 tgacgcgcag tcctttccaa atatcttcta atgaataggt ccagatacca ttttctagtt
    16561 tgttttccca ctcataacta ccagcggcaa taggagtaaa agatgaatca atcaatacag
    16621 ctttgatcct agttgatcca aactcaatcc ctaaagatgt acgaccatct atgacatcta
    16681 acatactttg cgcatttact tccttcataa tgatttaact ccctccttct cgtcaaacct
    16741 gatcaaaccg tctttttagt agcgcttaca atgattattg tatacttttg tacgtacatg
    16801 tcaagcggtt atttacaaga atacaataaa aaacacttgt tttgtacgta cataagttat
    16861 aaaatgaaat tgcttacaaa atctgatcaa ccgccttctc gaaattctag aaggagagaa
    16921 tacttatgaa aaaagaaaaa aaagtttcct catcgtttat ttattttttt ggcgcatttg
    16981 gcggcattct atttggatat gatattggtg ttatgaccgg cgccttacct tttttacagc
    17041 atgattgggg tttggcaggt aaagcttctt tgataggatg gatcacgtct tcggtcatgt
    17101 taggtgcgat ccttggcggc tctttatctg gtcttctttc tgataagctt ggacgacgaa
    17161 aaatgattct tctttctgca cttattttta tggctggttc tgtcctttct gcatctgccc
    17221 ctcacaatgg ttcttatttt ctgattgcag caaggattct tctaggttta gctgttggcg
    17281 ctgcatctgc cctagttcct gcttatatgt ctgaaatggc tcctgctaga ctaaggggaa
    17341 gattgtcagg gatcaatcaa gtgatgattg cttctggcat gctcctttcc tatgttgcag
    17401 attatttgct aaaagtgttg cctgaaacga tggcttggcg cgtgatgtta gggctcgctg
    17461 ctgtccccgc gctgatttta tttttcggtg ttttagctct accagagtca ccgcgttttc
    17521 ttatgcaatc tggccgcttg gaagaggcaa aaagagtatt gaactatatt cggacaccaa
    17581 aagaagctga acaggaattc gaacaaatcc agctgaatgt caaacaagaa aagacaaccg
    17641 gaacttcttg gcatactttg tttttagaaa aatatcgttc cttagtcatt gccggtattg
    17701 gtgtagcagt attccagcaa tttcaaggag ctaatgctat tttttactat attccgctga
    17761 tcgtagaaaa agcgactggg catgcagcga gcgatgcatt gatgtggccg atcatccaag
    17821 gaatcatttt agttgctggt tctttgctct ttttggttat tgcagacaag ttcaatcggc
    17881 gaactttatt gaaaataggc ggtagcgtga tgggactttc attcatccta ccagctgttt
    17941 taggaactgt actggatgct catacaaatt cgttgttgat tctcttgttt ttatgtattt
    18001 atgtcgcatt ttattcttgc acatgggctc cactgacatg ggtaatcata ggcgaaatat
    18061 tcccactagc tgtccgcggg cgagcatctg gcctagcctc ttcttttaat tggattggtt
    18121 ctttcctagt tggcttgctt tttcctgtga tgactgcttc tatgtctcaa gaaatcgttt
    18181 tcggcatttt tgggatcatc tgctttttag gggttttatt tattcaagag atcgtaccag
    18241 aaactcgtgg gaaatcttta gaggaaatcg aacaaagtgc atctaaaaaa acttatccta
    18301 aacgtatttc tatccatcat tcttaaagag gttgtgacag aagtggtcag cttcaagaaa
    18361 taagaaggaa ccgcttagtt tccagtcaca aggaacaatt tcaaaaaaca gcttctcatg
    18421 tttgatgaat attgtgactt gtgaccagtt ggtaggagaa caccgtttag tggagaggtg
    18481 gtgaaagaaa cgttcagcct tgagcgatga caaaagctct cacaaacatt gcgacgagta
    18541 accgtaggag caataagagg cggtaaaaac ccgtctctta tttttcgttt tctttcactg
    18601 tttcccgttc aactagtaca ggttcataga gaatatttgg ctgtgcctgt cctttttcaa
    18661 tcgttgtgac gatccaccgt gcggcatctt gtcccaactt ttcttttgga tgcgtcaatg
    18721 ttgtcaattt tttattccct aaatggctta aagaagaatc gtctgttcct acgatcgata
    18781 ggtcatctgg aatatgatag ccattttctg tcaacttatc tagcattaaa ctagctattt
    18841 gatcgttata gcaaatgatg ccatctaaag cttgttcttc tttcaaccgt tgtactgttt
    18901 tttcagcgat tgtttctcgt gtttccgtgg tgaaagtaat gatatcttcc gtcaaaaaac
    18961 gcacattata ctgttcacac gcgctgataa aacctttcat cctgtatttt ccttgaagat
    19021 cgtcgatttt tgtgatgaac aacagacgct gatgtccttt ttttatcaaa tattctgctg
    19081 ccataaaacc gactttcaca tcatctaaac aaatataaga ggcatttaac tcttcataag
    19141 cagcattgat catcaccatc ggaatgcctt gttctctcaa agcaacataa agtgctaagt
    19201 ttgggttata ctgattactt ttcgtaggct caacgatcaa accatctacc ccataagcaa
    19261 tcattttctt caaacactct ttctccaatt cgtgatcgtt attcgtactg gctaaaagca
    19321 atgaataccc agattctcgt aattcatttt cgattcctcg gataatcgac ggaaaaatat
    19381 aatcagagag atatgtggtg atgacaccga tcgtttttgt tctgttcaat ggtgtttttt
    19441 ctttcttata gctgtcatct acatatgtgc ccgacccttt ttctttacgc agatagccct
    19501 catttaccaa cacagcgatt gcttgccgaa tcgtatgtct gctgacgtca tatatttctt
    19561 gcagcttgaa ttctgaggga atggttgtat ttacttcgta ttcctcacga aggatctttt
    19621 gttttatctc ttctgcaata tcttgatatt ttgccatttg ccttgcccct ttttatttgt
    19681 acgtacatat atcataacga gtctgcaagg tttattcaag caaaaatcaa ggtttcttgt
    19741 gctcataagg catacggaac aggttcacca aattctggaa atccttgctc attccacgta
    19801 aagcattgcg cgttcgcgtg ccgattagga ttgtctaacg gatccccttt ttggttcttc
    19861 tctggtcgag catgatagat caaaatatca aagcacccgt cttctgaaac cgtaaacgag
    19921 ttgtgaccag gaccaaattg cttatttttt tcagaagaaa cgaatactgg ctccgataat
    19981 ttatgccatg attcttttaa taaaagattc ttgtgctcat cagcccacag caggcccatg
    20041 gcatagtttt catctgttgc actaccagaa tacgtaatga atattttccc attgcgagta
    20101 aggactgctg gaccttcatt tactgaaaaa ccccgttttt cccattcata ctcaggtata
    20161 gaaaggagga tctgcttccc ctttaacgtc caaggattat ccatttcgga tagataaaga
    20221 ttagaattcc cagtaatttc aggatcttgt tgggcccaca cataatatag ctttccttga
    20281 gaagagaaag ttgttccgtc taaactgaaa gtttcccttt tcgtttttat ttgtcccttt
    20341 tctatccaat ctgtattcat tggatctata tgctgatttt caagtacgaa catgcggtga
    20401 tgatgagatt catcacgtat agttttatga ttgcttgcag caaagtaaat ataccatttt
    20461 ccattgatat aatggacttc aggtgcccag atcaattcac tcatcggtcc ttcttcatga
    20521 gcctgccata caatcaggct tgcagcatgt tctaattcat tcagtgactt tgcccttctt
    20581 aattcaatca cttggtagcc tggaaccgat cctgtaaaat aataataacc atctgtatgt
    20641 ttatataccc aaggatctgc ccgttgccga acgataggat tattataaag tgtcttcatc
    20701 ataacaaatt tcctcacatt ttctttattc taattacatt ccaagagtat ttttctatcc
    20761 ctacatttaa ttcgtcatgt ttttctattg aaaaatagtt tatttcaggt tttatttttt
    20821 ctggttcttc acgtgtattt ttgtccaaca gatcgctact ggttaatgtg tagtgctctc
    20881 cttgatctag caaattgaaa tcatgttcaa tcgttatctt ttgttgttca tttgtgcgat
    20941 tgatcaagaa aatagtcagc tgattgccgt tatctacgat cacactgtcc acataaggaa
    21001 cattttttat cttttcacaa tcataaacag ggcaaatgat ttgttgtttc aatacttgac
    21061 ctttagcata tttcgataag tgttgcatca cataataagt tggattcctc catgcttgtc
    21121 ctttagcatc tgtcagtatt aaaggtattg tgtttactaa caatgattga caactaatct
    21181 ttacgcgatc tgcgtgtcgt aaaatagcca gccctaatga accaaataat aatgtatctt
    21241 ccatagtaaa gtaacaccaa tcgatgggac taccgacttc aaaatcccta tgtctttttt
    21301 ccggttgggc atgtacattc cattcatcaa acgctagata cattgttttc tcacttctca
    21361 aatatccttt tacataatca caagtagcaa cgatttcttc aatttgacga tcgaatcgat
    21421 aagcttttgc aaggtatgtc gctgtatcat cacgctcatt tttaggaggt ttagtcaagt
    21481 cttcttcatc atacttatca atatagttat gcaaagctaa ataatctaca ttctcataag
    21541 cttcttctaa tgcgatacga tcccattcag ggtacgatgg taaacgtggc gtagagcttc
    21601 caactaagat tgtttcaatt gaatcatcca ctaacttcat gacttttgag gcttcatttg
    21661 ctaacctacc atattcataa gcactcttag cccccacttg ccatggaccg tctagctcat
    21721 tccctaaaca ccataattta acgccaaaag gctttttttg cccatttttt attcttaaat
    21781 cactccagaa ggttcctttt ggaaaattac aatattctac taaatttttt gcttcatcaa
    21841 ttccttttgt tcctaaattc accgtgaata tcggtttcgc ttgtacatga ctgatccatt
    21901 taaaaaattc gtggagtcca aaagaatttg gttctatacc tcgccatgct aaatcaattt
    21961 tttttggtcg ttgttctttc ctacctacag tatccaccca atcataacca gatgtaaagt
    22021 ttcctccagg atagcgaatg acacctaatt ctaattcctt tacataagag aggacatctt
    22081 gtctaaaccc gtcaccatca gctgtttcat ggcccggttc ataaatccct gtatagacga
    22141 cacttcccat atgctcaaca aatgagccaa acagccgaga actgatctct cctaccgtaa
    22201 aatcctctct gatttttact ttcacttctt cgtccaacag ctttccctac ctttcatttc
    22261 ctatagtcaa gataatagat acaagtttcc atacgccacc agcaaagata aaaataaaag
    22321 gagggaaaag aaatatgagc agtacaaatc ctaataacaa tacgcaacaa agaatatttc
    22381 tcaaaaaatc aaaaaatact ttccatgcac tttgttcaaa aatttgtaaa aatgtgatat
    22441 ctttgttttc agaaatcaca tatatccagg ataaaagaaa catggaataa ataagtaaaa
    22501 ctacatatcc actaattgaa agtacgattg tctgttcttc taataattga ttaacaagaa
    22561 aataaataat gatcgttaac agactaagga acaagttctt accaaaatgt tgctttattc
    22621 cttgaaagaa aaaataaatg atatttcctg atccctcacc tttccattgc gccattacat
    22681 aaaaaacagc acatgtagca ggcacgatag taataacagg caaacacacg attacccaga
    22741 gaatacctaa taagatgtaa tcagaaacca caatcaactg tccataaaat ttagtctcaa
    22801 ctattttcat cttcctcact ctttcattcc agacatagct gttccttgaa caaaatattt
    22861 ctgtgcaaaa atataaacca ctagtaatgg cagcattgaa ataaccgttc cagccattac
    22921 aggtccataa aaagaaatgt tctgccctgt aaataaagct aaccctgctg gtaaagttct
    22981 catttttgaa ctacttgtca atatcaatgg ataaagcaag tcattccaac tattcatcag
    23041 cgtgaaaatc cctaatgata aaagagtagg tttgctcaat ggcaacatga ttttcaaaaa
    23101 aatattaaac tcacttaatc catcaatgcg tgctgcctct tccaaagaat ctggtaaagt
    23161 aataaaaaag gaacgcatca tgaagatacc aaatgcagtt gtcatacgag gaatgatcaa
    23221 acctgcataa gtatcaagca tgccaagcaa attgacttca ataaatagag gaatcatgaa
    23281 gacttggaat gggatcatca ttgtcagcaa aacgaaatag aacaaaactg attttccttt
    23341 gaaacgcata cgagcaaatg cataccccgc aagagaatca aagaatacag aagtgatcac
    23401 tgtccctcca gcaaaaatca ctgtattttt gatgtaatct aataaaggga tcgttgtcca
    23461 aaccttaata tagttatcta gggtatagct atctgcaaat attctaggcg gaaatgagat
    23521 aatgtctttt tcgtgtttaa aagaagaaat aatcagccac aaaaaaggaa acgcaataac
    23581 caacaatata actagcatac taatcgttcg aatgattttt ttaccactaa ctgcttttcg
    23641 actattcaca gtaactgtaa tcgagctttc tggtgccata caggtttccc tcctatttca
    23701 tttgtttttc tttccgtgac atgtacacat tagtggagat agctatagtt gcaacaatga
    23761 taaacaaaac cgaagacagt gctgatgcat agcccaattc ataaggtgct gtaaatcctc
    23821 gactgtaaat atattgaaca gctgtttctg tcttaaattg tgggccacca gcggttgcaa
    23881 catatacttg gtcaaacact tgcatcgaac caatcagatt cgttaaaata caaaaaccta
    23941 aggagggaac gatctgaggt agcgtaatgt taaaaaactg ctgtcgattt gttgcaccat
    24001 ccatttgagc agattcatac aaagagggag agattccctg aatagcagtc aagagaatcg
    24061 tcatagtaac accaaaattc ttccaaacag tcatgatggc taccgtcggc atagcaaggg
    24121 ttggatcgcg aaaaaattga gggatcggca agccgacttg tgaaaacaag taaggaatcg
    24181 cccctacatt cggatcaagc aacatagaaa aaataatcgc aattgacgtc aatgaacaaa
    24241 taactggaat gtaaaatgtc gaacgacaaa aacgattgaa tctggtattt ttagataatg
    24301 ctactgctgc taataaacca acgataattt gtgttggcgt ttcgagtacg gtgaaatata
    24361 aagaatgaat caacgaatta actgctcgtt catcactaaa tatacgtgtg aaattttcaa
    24421 aacttacaaa atttcttgat gtaaagaaaa tattcaaatc tgtaaaacta atagcaaatg
    24481 ttcctaaaag cggtatgaca gtaaataata ataaaactaa aaaagatgga gttaaaaaca
    24541 aatacgcagc acgatcagga cgattccaat atttggtgaa tccgttcata tagtccacct
    24601 catctttcat tttgataaaa tatacaatct tggtttaaac gaatctgaat aagatatccg
    24661 aacaatcgct gtgatttttg caataacacc tatctgcaaa tactaggtcg atcattcaga
    24721 tatctgtatc ttgccaacag tctatcctgt ataaagttct gtactatttt tgcagtaatt
    24781 gattgatggc atcatctgca tcgttcatga gtgtctcagg atcttctcct gctaataatt
    24841 tttcaatcag tggattgatg atatcgttat tgatcgcaga gatttgggta aaacctggta
    24901 aatatggctc tgcatagtcc atttgtttcg ataattcaga tacaatagga tcatttttta
    24961 cttcttcatc ttttgctact gaatccaagt aggcaggaaa accattttcg atactccatt
    25021 ttttaccaat ttcagttgta ttccaatatt tcaaaaactc atagacagct tcttttttat
    25081 tattatctgt acttgccgga atggcatatc ctacgccatc taaaatagct gtttttttgt
    25141 ctgaatcact cccttgtggt aaggtagtca cattataatc aatctcgttt gctttcaatc
    25201 cgttatttaa ccatggccca ttgatttcaa tggctagttg accagcattc attaaattgt
    25261 ccatttcggc gccagaaatc gattctggac cagtcttgtc tacatggatc attttttgga
    25321 tcgcttttaa cgttttaagg ttctcaggag acgcaaattc agacttagta taatcatcgt
    25381 tcactaattt accgccattt gctaacatcc agttatataa cggtgcagtt ccatccttat
    25441 tgaagacaaa acctgataca tttttactac tatcagttaa ttttggtgcc atttctgtca
    25501 attcttccca agtagtaggt ggttgttcag tatctaatcc agcttgttta aacaaatcat
    25561 tattccaata catatacatg ccttgtacct gcatggggac aaaatactgt ttaccttcta
    25621 cttcacctaa ttcaatagcc gtttctttaa aatcagattt atctactcca tcatattccc
    25681 agaaatcgtc taatggttgt accgctccgt tttctacata ctgtgcaaaa tctggataat
    25741 ttaatgcaat aaagtcaggt gcttttttag aagaaatcgc tgtaggaagt ttttcattca
    25801 aattggccca agtcatgata tccatttcaa ttttgatgtt tttatcattt gttttattaa
    25861 attcattaac gatttcttgc aatatttcac catcagatgc agtaaaccca ttccaaaaag
    25921 tcaaagtcac ttctttatca gaatcagatt ctgctgaact tccgccaccg caagctccta
    25981 aaatacctgt tgcagctacc aacaaagagc caattaacca ttttttacct ttcatttttt
    26041 gttcctcctt catctgataa acgacatctg accacattcc aagatagcgg tttcaataaa
    26101 caattgattt tcccatcttc agcttttaca tttgttgaat gacttggttt tacttgttca
    26161 cttttaggaa gattagttgc ttttgtatca aatccactca tttcagaaaa atcaatcact
    26221 ccctctaatg ataattcaga caagtgaaca gttagattca tttcttcttc tccacggtta
    26281 actgcaaata tgacaagttc tttgtcattt attacggcta tactttctaa atacggcact
    26341 tcttcaaaat cttccgtcgc atagtttgct gaacgaattt gtgggcttaa cacctctcct
    26401 tgtccataca cagcagcttg cataaatgga taatagatcg tctgtctcca agcttctccg
    26461 tttgtttctg tcctgattgg tgcaataacg tttaccaatt gagcaagaca agcaatcttt
    26521 actctatctg catgtttcaa taaagtgatt aatagacagc ctaatagtaa tgcatcttca
    26581 aaattatagt tatcttctag taaaggagga gcaatctgcc aaggcgctgc ttgctcatct
    26641 tgttccgctg aatgatacca aatattccat tcatcaaagg acaggttgat ctgcttctta
    26701 ctatgtttct tcgccttgac tgtatcgcac atcgctgtca cttcagaaat gaaccgatcc
    26761 atgtctaagg aacgtgcaag ataatgagaa agatcatttt tttggttgcc ataatattga
    26821 tgcagagaca aataatctat ttgatcgtac gcttgatcaa gaactgttaa ctcccaatca
    26881 ccaaatgtag gcatttgccg attcgaactt ccgcataaaa ccacctcaat tgaatcatct
    26941 acgagtttca tcgccttagc cgtttcctct gctaaccgtc catattcttc cgctgtttta
    27001 tgtccaatct gccaaggacc gtccatttca ttccctaaac accatgtctt aattccaaaa
    27061 gggctctctt gaccattcga acgccttaaa tcactccaat aagttccttt aggaaaatta
    27121 caatattcga ctaaattacg agctgcatct actccacgag tacccagatt gacagccata
    27181 ttgatttcgg cccccacatg ctgacaccag ttataaaatt catgaatccc aacttcattc
    27241 gtttcaattg tccgccaagc taaatccaac cttctaggtc tatttgctat gggcccaata
    27301 ctgtcttccc aacgaaatcc tgacacaaaa tttccaccag gatatctaat catgggaatg
    27361 ttcaattctt ttaccaagtg aatgacgtct tggcgaaatc cacttttatt agctgatgga
    27421 tgatctggct gataaatccc ttcatatact gctcgtccca tatgttcaat aaatgaactg
    27481 tacaaccgcg gatcaacttt gcctacacgc ttgacactac taatcaacat tgtggcttcc
    27541 ataactgacc tcctttaatt tatacatgaa taatacacct cgttgaaagc gttgtcaata
    27601 atataaatgt taaacttact tattttttat cgattttaat taaccaaaat attaataatg
    27661 attaaaacaa ataatttaat ataactttta ttaacaaata tgttaatata taagaaaaac
    27721 cttttaagag gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga
    27781 agcattagct agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa
    27841 tgtcaaagat ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa
    27901 aaagctagag gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag
    27961 ccttcgtgtc gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac
    28021 gaaagaaaca tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg
    28081 tttagctacg atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc
    28141 tcgaaggatg gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc
    28201 taatttttta aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc
    28261 tgaatttcct ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt
    28321 ggagctagga acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc
    28381 tgattggtat ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa
    28441 acacttgacg aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa
    28501 agagcaagac acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg
    28561 ctgtacttta tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta
    28621 ctacagctga tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg
    28681 gacggctgtt tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat
    28741 gtcgtttatg ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa
    28801 cttcttttcc ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat
    28861 ctgcaatcat tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt
    28921 ccggtgcatt caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg
    28981 gcaaaatgtc ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag
    29041 catgatctgt ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca
    29101 tacgttgacg tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc
    29161 ttaatacttg tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg
    29221 gctggataaa gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa
    29281 gacgaacagc gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg
    29341 ttagttgcgc caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa
    29401 aagttttaag aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta
    29461 tttcttcttt tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca
    29521 ggctcattta ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg
    29581 ttccatgata gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg
    29641 cggattcaag tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc
    29701 tttgatttcg tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg
    29761 taaaactggg cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg
    29821 gaacatgaag atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc
    29881 ttcagatttc ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt
    29941 tactttttct gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc
    30001 gatcgatact agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt
    30061 tgtcccaaca ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc
    30121 ttcatccaag tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg
    30181 ttcccgaaca cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa
    30241 gttgattttg tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa
    30301 cgtatcataa gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa
    30361 tgcttcataa tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg
    30421 tatcccaaat atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc
    30481 cgctctagtt ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa
    30541 tagtggatct tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg
    30601 gaaattgacc atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc
    30661 cacttggatc aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt
    30721 tgctttaaat tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc
    30781 acgcaagtaa gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg
    30841 ttccaatcct gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc
    30901 aaaaacagca tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg
    30961 atatagatcc gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta
    31021 ctcctcttgg caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga
    31081 aatcttccat tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac
    31141 tccatctgct aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta
    31201 ttttattcaa tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata
    31261 aaataagtag attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata
    31321 taaccaaatg tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt
    31381 tccttttttg cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt
    31441 tggaaaggcg aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc
    31501 acacgttttt aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat
    31561 ggcgagacat caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt
    31621 taccataaag atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat
    31681 cactacaata tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt
    31741 cactcctaca tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata
    31801 gtcgatttct tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc
    31861 atatgtgtct accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat
    31921 agccgtattc ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt
    31981 actattcaca ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta
    32041 gatccaatct taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa
    32101 cctttaaaga caaaaggatt taccttttat cacttaatag tataacataa tttcatgaga
    32161 aaaaaataca attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca
    32221 ggaggaaaaa taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg
    32281 agaaccttgc gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca
    32341 ggttatcgga tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga
    32401 acaatggcat tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac
    32461 cagaaagctt taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc
    32521 ctcttgacaa ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac
    32581 aaagaaaaat ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga
    32641 caagagattc gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg
    32701 aatatggata aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat
    32761 ctcactcttt acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct
    32821 cataagaagt ggttatctta cagctggcct gaatatcagc caaaagcgca taaaggacta
    32881 ggaatgatgt acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat
    32941 ggtgcagcaa aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc
    33001 aacaaaaaag tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt
    33061 ttatgctaac ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat
    33121 atattcatcg ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa
    33181 acaattgaaa tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa
    33241 gaagctcgct gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat
    33301 agctttgcaa gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa
    33361 gctctttaaa aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa
    33421 caagtgattc atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt
    33481 tataacttag cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct
    33541 tcaaataagt aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct
    33601 gttttccctt gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat
    33661 catctagtgt attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt
    33721 cttcaagata ccttcgctct ttttctttct gatagcccgt cggtaaaagt gtcactttgt
    33781 aatcataatc aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat
    33841 tggtataagg cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat
    33901 ttgcattagg gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct
    33961 catctacata tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga
    34021 gctttgcgat atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc
    34081 ttgtaaatag tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg
    34141 cgaaaaactg atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt
    34201 cattggtatt tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac
    34261 ggatcccttg aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc
    34321 tacataagta ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg
    34381 tctcatagca aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca
    34441 aagtctc
//
LOCUS       DAXLPI010000016        33503 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXLPI010000016 DAXLPI010000000
VERSION     DAXLPI010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.016"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(9..125)
                     /locus_tag="ACF3T5_000687"
     CDS             complement(9..125)
                     /locus_tag="ACF3T5_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFD3631320.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(198..1154)
                     /locus_tag="ACF3T5_000688"
     CDS             complement(198..1154)
                     /locus_tag="ACF3T5_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFD3631321.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1325..1693
                     /locus_tag="ACF3T5_000689"
     CDS             1325..1693
                     /locus_tag="ACF3T5_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFD3631322.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            1863..2294
                     /gene="mraZ"
                     /locus_tag="ACF3T5_000690"
     CDS             1863..2294
                     /gene="mraZ"
                     /locus_tag="ACF3T5_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFD3631323.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            2315..3274
                     /gene="rsmH"
                     /locus_tag="ACF3T5_000691"
     CDS             2315..3274
                     /gene="rsmH"
                     /locus_tag="ACF3T5_000691"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFD3631324.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            3279..3680
                     /gene="ftsL"
                     /locus_tag="ACF3T5_000692"
     CDS             3279..3680
                     /gene="ftsL"
                     /locus_tag="ACF3T5_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFD3631325.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRRVK"
     gene            3681..5873
                     /locus_tag="ACF3T5_000693"
     CDS             3681..5873
                     /locus_tag="ACF3T5_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFD3631326.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            5899..6861
                     /gene="mraY"
                     /locus_tag="ACF3T5_000694"
     CDS             5899..6861
                     /gene="mraY"
                     /locus_tag="ACF3T5_000694"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFD3631327.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            6875..8236
                     /gene="murD"
                     /locus_tag="ACF3T5_000695"
     CDS             6875..8236
                     /gene="murD"
                     /locus_tag="ACF3T5_000695"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFD3631328.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            8262..9350
                     /gene="murG"
                     /locus_tag="ACF3T5_000696"
     CDS             8262..9350
                     /gene="murG"
                     /locus_tag="ACF3T5_000696"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFD3631329.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            9365..10585
                     /locus_tag="ACF3T5_000697"
     CDS             9365..10585
                     /locus_tag="ACF3T5_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFD3631330.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            10741..12066
                     /gene="ftsA"
                     /locus_tag="ACF3T5_000698"
     CDS             10741..12066
                     /gene="ftsA"
                     /locus_tag="ACF3T5_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFD3631331.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            12089..13330
                     /gene="ftsZ"
                     /locus_tag="ACF3T5_000699"
     CDS             12089..13330
                     /gene="ftsZ"
                     /locus_tag="ACF3T5_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFD3631332.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            13347..14024
                     /locus_tag="ACF3T5_000700"
     CDS             13347..14024
                     /locus_tag="ACF3T5_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFD3631333.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            14038..14637
                     /locus_tag="ACF3T5_000701"
     CDS             14038..14637
                     /locus_tag="ACF3T5_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFD3631334.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            14718..14939
                     /locus_tag="ACF3T5_000702"
     CDS             14718..14939
                     /locus_tag="ACF3T5_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFD3631335.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            14988..15770
                     /locus_tag="ACF3T5_000703"
     CDS             14988..15770
                     /locus_tag="ACF3T5_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFD3631336.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            15791..16498
                     /locus_tag="ACF3T5_000704"
     CDS             15791..16498
                     /locus_tag="ACF3T5_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFD3631337.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDIGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            16760..19558
                     /gene="ileS"
                     /locus_tag="ACF3T5_000705"
     CDS             16760..19558
                     /gene="ileS"
                     /locus_tag="ACF3T5_000705"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFD3631338.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(19607..21133)
                     /gene="zwf"
                     /locus_tag="ACF3T5_000706"
     CDS             complement(19607..21133)
                     /gene="zwf"
                     /locus_tag="ACF3T5_000706"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFD3631339.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(21148..21795)
                     /locus_tag="ACF3T5_000707"
     CDS             complement(21148..21795)
                     /locus_tag="ACF3T5_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFD3631340.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(21979..22308)
                     /locus_tag="ACF3T5_000708"
     CDS             complement(21979..22308)
                     /locus_tag="ACF3T5_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFD3631341.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            22485..23213
                     /gene="sfsA"
                     /locus_tag="ACF3T5_000709"
     CDS             22485..23213
                     /gene="sfsA"
                     /locus_tag="ACF3T5_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFD3631342.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            23229..24242
                     /locus_tag="ACF3T5_000710"
     CDS             23229..24242
                     /locus_tag="ACF3T5_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFD3631343.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            24242..25519
                     /locus_tag="ACF3T5_000711"
     CDS             24242..25519
                     /locus_tag="ACF3T5_000711"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFD3631344.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            25582..28284
                     /locus_tag="ACF3T5_000712"
     CDS             25582..28284
                     /locus_tag="ACF3T5_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFD3631345.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            28436..28753
                     /locus_tag="ACF3T5_000713"
     CDS             28436..28753
                     /locus_tag="ACF3T5_000713"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3631346.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            28783..29103
                     /locus_tag="ACF3T5_000714"
     CDS             28783..29103
                     /locus_tag="ACF3T5_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFD3631347.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            29211..30671
                     /locus_tag="ACF3T5_000715"
     CDS             29211..30671
                     /locus_tag="ACF3T5_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFD3631348.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            30739..30960
                     /locus_tag="ACF3T5_000716"
     CDS             30739..30960
                     /locus_tag="ACF3T5_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631349.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            30991..31173
                     /locus_tag="ACF3T5_000717"
     CDS             30991..31173
                     /locus_tag="ACF3T5_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFD3631350.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            31173..31586
                     /locus_tag="ACF3T5_000718"
     CDS             31173..31586
                     /locus_tag="ACF3T5_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFD3631351.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            31709..32890
                     /locus_tag="ACF3T5_000719"
     CDS             31709..32890
                     /locus_tag="ACF3T5_000719"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3631352.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(32961..33125)
                     /locus_tag="ACF3T5_000720"
     CDS             complement(32961..33125)
                     /locus_tag="ACF3T5_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631353.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            33279..33352
                     /locus_tag="ACF3T5_000721"
     tRNA            33279..33352
                     /locus_tag="ACF3T5_000721"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33313..33315,aa:Arg,seq:cct)
     gene            complement(33421..>33503)
                     /locus_tag="ACF3T5_000722"
     CDS             complement(33421..>33503)
                     /locus_tag="ACF3T5_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFD3631354.1"
                     /translation="HIVRELEDQDKEKINTVFDSLYKNDN"
BASE COUNT        11183 a         5721 c         7081 g         9518 t
ORIGIN      
        1 ttttattttt acatgacact cattacagca gtcaatacaa cagatccaat cgttaaaatc
       61 agcattgtcc aaatgacaaa ttttgttact ttagcaaaag tacttgtttg ttttttttca
      121 cgcattgttt tcaacctcgt cattaattag ttgattcaag tgtacaatct tttttccatt
      181 tccacaagag ggctttttta taaaaacttc ccttttttta aataataggt agttacgata
      241 caaataaata atgttaatag aactagccat ataaaggcat catccctttc agcaataggt
      301 aacttaatgt tcattccata gatactgccg acgattgtcg gtatagttaa aataatcgtt
      361 aaagaagtta aaattttcat aatattattc agattattcg atacgattgc tgaaaaagtc
      421 tgattcattt gttctactag ttttaattga atgcgagaag tagtcattgc ctgctctgtt
      481 tccaccaata catcacgtaa atgatttttc aaagcatgat tttcttggaa aagtttattt
      541 tgcgctagtt ctttcaatgc atctaaatta gcagaagtcg cttctctgaa ataaacgagg
      601 cttttttgga tatccatgat ttgataaagt tgtttgtttt cagtggatac ttgaagtttg
      661 gcttccaaag tatcgcattg agattgaagc gtagctaaat aactgttgta agagagtgtg
      721 acctgccaaa tgaattgaaa gaagatattg agattaggcg aaatatcact ttttggaaag
      781 gtctggctaa agactttctt taaaaacagt ggttcatgat tcgtaattgt gataagcttc
      841 tttttgggcg tcaaaataat agagagcggg tatgtatcca cttgaaaata accgctagga
      901 cttgttttag caaaaggata ttgtaacaat aataacgctg gctttgtcaa ctttgattga
      961 ttgaattctt ctgtacggga attttctgca tcgtccaata tggaagtgat gtaatctgac
     1021 ggaatttcaa attgatgtgt cagctgatga atctcttctt gattaggacg ttctacacaa
     1081 taccagatcg tttggtcagt ttcctttccg gtattacgca gtttctcatt atctaatgta
     1141 aaatatttga tcatgatttt cctcctgttg cctgtttctt ttattataac ggatagggaa
     1201 ttggaataca tggaatgtgt ttgtaagata tagaatataa ctgcataaat ttaaataaaa
     1261 tgaaacagtt gtgtaaagta tatacagtat acttgaagca acgataaaaa aggagtttac
     1321 gttcatgggc acattcacac cacttatctt gttttggtat atttacccgg taatcgtttt
     1381 gtttggttgc caatttcttg taaaattatt ttcgttaaat cgtcgtttta aaataaaagc
     1441 ccctgatttg gcgattcctt ttttgtgggg cggtatgcat gcattgtcta gaaatacagg
     1501 gacaatttct atcttaccgt ttttctttat ttctgttttg ctaatgggaa tttgtattgc
     1561 tgttttccaa gcatattatt atgaagaaat tatttatccg cgttatttca agatgacttg
     1621 gcgtttggtt tttctgttga caatcgtctt gtacgctgtc ttgatcattg tgaacatact
     1681 ttcttaccta tgaaaaaaat agaaaagctg taaaaagtct tttaaaaggc tttttgcagc
     1741 ttgattttta aaaaaatgtt tatgaataaa aaaagttgtt cccggatttc ccagaaaagg
     1801 tggtagaaag tgggggattg tggtagacta attttagtta gtggaggaat ggggggcttt
     1861 caatgttgat gggtgaattc caacataata ttgatgcaaa aggacgttta atcgtgccct
     1921 caaaacttcg tgaagaactt ggcgaaaagt ttgttctgac acgcggattg gatggctgtc
     1981 ttttcggcta tccgatgagc gaatgggaaa atctggaagc taaactgaac gaaatgccgt
     2041 tggctaaaaa agacgcacgt acttttgtgc gattcttcta ttcggctgcc actgaatgcg
     2101 aacttgataa acaaggacga atcaatatac ccagcacgtt aagaaattat gcagctttga
     2161 caaaaggttg cgtagtcatc ggtgtatcta accgaattga aatttgggat gagacacgat
     2221 ggcaagattt ttctgcagca gcagaagaga actttgacga aattgctgaa agtatgattg
     2281 atttcggact ataagaatag gaagaggctg actaatgact gaaacatttc aacactatac
     2341 agtcatgttg aaagaaacgg tagatggatt gaacatcaaa ccggatggcg tttatgtaga
     2401 ctgtacactc ggcggtgccg gacatagtga atatttatta agccaactaa gttctaaagg
     2461 gcatctgtat gcgtttgacc aagatcaaaa agcaatcgat cacgccaagc tgcgtttagc
     2521 ttcttatgtc gaacaaggta tggtcacatt tatcaaagcg aacttcagag aattagaaga
     2581 acgcttgagt gagcatgtag ataaggtaga cggcattttg tatgatctag gggtttcctc
     2641 tcctcagcta gatgaagcag aacgtggatt tagttatcat caagatgcac cgttggatat
     2701 gcgcatggat cagtcagctc ctttatccgc ttatcacgta attaatgaat attcctacca
     2761 tgaattagta aagattttct tccgctatgg cgaggagaaa ttttccaaac aaattgctcg
     2821 tgaaatcgaa cgagtacgaa aaacaaaacc aatccaaaca actggtgagc ttgtcgaaat
     2881 cattaaacaa gtcatcccag ctcccgcaag aagaaaaggc gggcatcctg caaaaagaat
     2941 ttttcaagct gtgcggattg cagtaaatga cgaactcggt gccgaagagg cctctttaga
     3001 acaggcaatc cggttattga aagtcaatgg aagaatcagc gtaatcacat tccattcatt
     3061 ggaagacaga attgtcaaaa gtatgtttaa agaatatagt acggtacaag atctaccacc
     3121 gggacttcct gtcatacctg acgaattcca accagaactg aaagtcatta ctcgtaaacc
     3181 aatccttcct tcagaggaag agctaacaga aaacaatcga tcacgcagtg caaagttacg
     3241 tattgcagaa aaaataagaa tgaaagagga gtagaatgat ggcagaatta aagaaaatgc
     3301 aagattacga atatgattta cctgaattgg tggatcagcc ggaacaacaa atgcctgatt
     3361 cttcttcgaa tcaagaaatc attcttccac aatcaccggc aaaacgatta aagcagattt
     3421 ctgctttgga aaaaattgct gtggcaagcg tggcgattgc tattgttgct ttatgtgttt
     3481 taacagttat gctgcgcaca aatatcagcg gagctgaaaa agaaatcacg tcaatacaaa
     3541 cagaaatcaa tgataaaaag caagaaaaaa cgagtttaga acaagaaaaa aacgaacttt
     3601 ctcgtactga acggattaaa caaatcgcag aagaaaaagg attgtctatt aatgacgaca
     3661 atttaaggag agtgaagtaa atgagtctta aaaataaaat tcggcggttt atggaaaaga
     3721 aaaatctaaa tccgatgaac aatcgcaaaa aagtaggtat cattttattt gctacgagta
     3781 ttggattgtt ctttttattt gccgtccgtt tctcgtatat cgttattgga ggtcatgttg
     3841 ctggtacctc attagctgaa aaaacaaaac agttgtacca aggaagtgaa gtagtaaaag
     3901 caaaaagagg aacgatttat gatcgtaacg gtgtggcgct cgcagaagat gcctcctctt
     3961 attcaattaa agcgatactc tcaaaaacat atacctctgg ggataaaaaa ctgtatgttg
     4021 aagaaaagaa ttttgataaa atagcagaaa tcctgcataa gaatctttct atagacaaaa
     4081 aagatgcact gaatatttta gaggacggcg caaaaaaaga gctgtatcaa gttgaatttg
     4141 gtagctatgg gaaaaatatc tcccaagaaa ccaagcaaaa catcgaagcg gacatgaaaa
     4201 aagaaggtgt cgctggtctt tattttgtcg accatcaggc tcggatgtat ccaaatggcg
     4261 tcttttcttc tcattttatt ggatacgcag ttccagacaa agatgagaat ggcttagttg
     4321 gtaaactagg tttggagtct gcttacaatg atatcttgag cggtaaagat ggtaagatta
     4381 tttatcaaaa agataatttt caaaatcctc tgccaggtac agtggcagaa gaagaaaaag
     4441 ctgtcgacgg acaagatatc tacacgactt tagacagcag actacaaagt tatttagaaa
     4501 cactgatgga tcaagtaaat gaagaatatc agccagaaga attgacagca gtcctgatga
     4561 aagcaaaaac cggagaaatc ttggcaatgg gacaacgacc tactttcaat cctgaaacaa
     4621 tggaaggatt gactggaaaa gatgctatct ggcgtaattt cttggtacaa gatagttatg
     4681 aaccaggatc aaccatgaaa gttttcacta ctgcagctgc tatcgaagaa ggagaattca
     4741 acgagaatga aaccttccaa tctggaaaaa tccaagtaga agatgctacg atcaatgacc
     4801 atgactttgg tgaaaaaggt gtgctgacca tgcgtcaagc tctttcatgg tcaagtaacg
     4861 tcgggatggt catactggaa cagcgattag gcggacgatg gtataactat ttacaaaaac
     4921 tgggatttgg acaaagtact cattctggat tagatgatga agtaaacggt gctttaccta
     4981 cattaaatat tgtcgaccga gcaatgagtg cttatggaca agcggtaggt gtgacgaatt
     5041 tccaaatgat gaaagctttc acttctattg cgaataacgg tacaatgatt caacctcgat
     5101 atatcagcaa agtggtggat ccccaaactg gagaagagcg aacaactcaa acagaagttc
     5161 tagggcaacc tttttcaaaa gaaacaacag aaaaagtccg tgaatatatg agagatgttg
     5221 tcgaaagcga aaactacggt agtgcttatg gtgtatatag tgtacctgga tacaatgtat
     5281 cggcaaaaac cggaacagcc caaatcgctt ctgatacagg gggatatcaa acaggagata
     5341 cagcctacct gtattcgatt gtggagatgg ttccttctga agatccagac tatgtcttgt
     5401 atctaacaat gaaacaccct aagacatatg atcgaatggc attggctaaa atcgcaaatc
     5461 cattgatgaa acgggcaatg gattttaaag aaactgaaga agattcagat acagaaacaa
     5521 aaacagaaaa agtatccgta gccgattatc gtaatcttga agcggacgtt gcagcagctg
     5581 atgctcaaaa aagtggactg caacctgtag tgatcggtaa tggtaaaaaa gtacaaaaac
     5641 aatctactgc caacggtgat cagttgatct ctggagaaaa actgatccta tatactggtg
     5701 gtgataagct gatgcctgac gtaacaggat ggtcaaaagc agatatcatg aagctgggga
     5761 aaatcctcgg tattgaagtt agttttgatg gagatggtta ttgtgtcaaa caagaacttg
     5821 ctccatatga aaaaatcact gaagacaaac ttagttttac tttagaagaa taagaagaga
     5881 aataggagag aaaagagcat ggaatggaca caagcattga ttcccattgt cagcagttgt
     5941 gcaatgacaa ttgccgcaat gccgctattt attggttatt ttcaaatgaa aaagcaaggt
     6001 caggcgattc gtgaggaggg accaaagtgg cacaatagca aagccggaac tcctacaatg
     6061 ggcggcttag tgtttctgat cggctctatt ttgacaggaa tttgggttgg agcttggcaa
     6121 aaacagctaa ctcctactct tttcatcttg ctttttgtat tagcgttata cggagtaatc
     6181 ggctttcttg atgattttat aaaaatattc aagaaaagaa acatgggact gaactcgaag
     6241 cagaaattga ttggacagat tctaggggga atcatctttt atcttgttta ccgttctgaa
     6301 ggttatccag gtgtattgaa tttcttcgga ctagatctac ctttaggaat catttatggt
     6361 attttcgctc tcttctggct tgttggattt tcaaatgcag taaatctgac agatggcatc
     6421 gatggattag ttgctggctt aggaagtatc tcttttgcag cttacggtct aattgcttgg
     6481 catcagcatc agtatgatgt attaatcatt tgcttaagtg ttttaggagg tttactgggt
     6541 ttcttcgtct acaacagaaa acctgccaag atttttatgg gagatgtcgg ttcattagcg
     6601 ttgggcggac ttttagcggc aatttctata atgctaaacc aagaatggac attgcttttg
     6661 attggtttga tctatgttat ggaaacagca agtgtgatgc ttcaagttac ttcctttaag
     6721 cttacaggaa aacgaatttt taaaatgtct ccaattcatc atcactttga aatgtgcgga
     6781 tggtcggagt ggaagattga tacagttttt tggttgatca gcattgtgac ttcgttgatc
     6841 acattatgga tcgtttggta ggagatgacg ggaaatgaaa aaaatatcaa catatgaaaa
     6901 taagaaagtg cttgtcttag gactcgcaaa aagcggagta agtgcggcca aattattaca
     6961 tgaattgggc gcacttgtca ccgttaatga tggaaaacct tttgacgaaa atccggaagc
     7021 acaagagctg ctttctttag ggataaaagt aataacaggt agtcatccaa tcgaattgtt
     7081 ggatgaagaa ttttcattga tggtcaaaaa tccagggatt ccctacagtc atcctttagt
     7141 agcaaaagcc caagaaatgg gtattccggt aattacggaa gttgagctag cttacgaagt
     7201 agcggaatgc ccaattattg ggattacagg aacgaacgga aaaacaacga caacaacgat
     7261 gacaggacta ctactgaatg caggagcaga tcaaggaatt gcacgtttag cgggtaatat
     7321 tgggtatcca gcaagtggtg tagcgcaaga agcaaaatca gaagataaaa ttgtgatgga
     7381 attgtctagc ttccaactca tgggaatcac agatttccgc cctcatatag cggtgattac
     7441 caatatctat gaagcacata ttgattatca tggtacaaga aaagaatatg tcaaagctaa
     7501 atggaacctt caaaaaaaca tgacagaaaa agattatctg atccttaact ggaatcagtc
     7561 agaactacaa gaattagcgc aaagaacaaa agcacgcgta ctgcctttct cgacaaaaga
     7621 agtgttggaa gacggtgtgt atgctgacga ttactcaata tactataaaa aagaaaaaat
     7681 aatggaaata tctgaacttg gcgtaccagg taagcataat gtagaaaatg cactagctgc
     7741 tatttctgta gcaaaattgt atggtatatc aaatgaagct atacgagaaa cgttgcattt
     7801 tttccatggc gtaccacatc gtacgcaata tgtaggagaa atccaaggca gaaaatttta
     7861 taatgattca aaagcaacca atattttagc gactaaaatg gctctaagtg gatttgaaac
     7921 gtctaaggtc gtgttattgg ctggcggatt agacagagga aatacatttg atgaattaat
     7981 tccttcatta aaaggaatca aagcaatggt cgtatttggc caaacaaaag aaaagttgat
     8041 ggatgctgga aaaaaagcag gcatcgagac aatagtaaca gccgattctg tagaacaagc
     8101 agttccttta gcattagaaa acagtacaga tggtgatgta gttttattat caccagcgaa
     8161 tgccagctgg gatcaatacc caaattttga aactagagga aacagattca tggaagctgt
     8221 gaaccgatta aaatagaaaa agtagaagcg agcgataaaa aatgaaaatt ttggttacag
     8281 gcggaggaac agggggacat atttatccag ctcttgcctt tgtcaattat gtaaagactc
     8341 aagaaccgaa tgctgaattc atgtatgtgg gagcaaaacg aggtctagaa aacaaaatcg
     8401 tccctgatac aggaatgcct ttccatacct tggagattca aggatttaaa cgaaaaattt
     8461 ccatgcataa catcaagacg gtccgattgt ttcttaagag tatccgtcaa gcaaaaaaaa
     8521 tcctcagtga ttttcaacca gatatcgtta tcggtactgg aggatatgtt tccggagcgg
     8581 tagtgtacgc tgcagccaaa atgggtattc caacagttat ccatgaacaa aacagtgtac
     8641 caggtgttac caataaattt ttaagccgat atgtggatcg gatcgctctt tcatttaaag
     8701 atgcggcttc cttttttcct gaaaacaaag cagttcttgt aggtaatcct cgagcacaag
     8761 aagtagcaga tactaaaaaa tcagatattt tgaaaacatt tgatttggat ccagaaaaga
     8821 aaacagtctt gatttttggc ggtagtcaag gagcacttaa aatcaatcaa gcagtatcag
     8881 tttttttaag tgaatatatt ccttcttctt accaagtttt gtatgcttca ggagatagat
     8941 attatgaaga gatcctaaat aaaataaatc ctgaaataca aaatattagt gtccagcctt
     9001 atatcaaaaa tatggcagag gtcatggctt ccagtgatct gctggtgggg cgtgctggtg
     9061 caacttcgat cgcagaattc acagctctgg ggttaccggc agtgttggtt cccagcccat
     9121 atgttacgaa tgatcatcag actaaaaatg caatgagtct tgttcacgca ggagcagcaa
     9181 aaatgattgc agataatgaa ctaacaggtg aatcactaag tcagacagtc aacgaaatca
     9241 tgggtgatga agaattgcaa aagcaaatgt gccgagcttc taaagaacaa ggaatccctg
     9301 atgcttcaaa acgtctttat gacttagtaa aacaaataat caaaaaataa aggcggtggt
     9361 cttagtgagt aagaaaaaag aaccaaaaaa attgtctcat ccgtcttctg atgaaaacaa
     9421 tttgactcct tggcaaaagg ctaaccgaaa atatctggca gaacaagaga tgaaaaacca
     9481 agagccaaaa aaagaagaca taacattaga tacacccctt tcagattctg aagaagaaaa
     9541 aacggttcaa gaggaacagc aagaagaaag ccagtccgag aaaaaaatcg aattttttga
     9601 agagatcaag caaaagaaaa acggtggacc atataacggt tcttttttga accggctgcc
     9661 caatttaaaa agtcaacggc acaaagtact atttcgtcgg ttagctttga tcatcactgt
     9721 attgggtata ccgctgattt ttttgatcta ttatgtttct ccttatagca aattacaagc
     9781 agttgcagta tcaggcaata agacagtcaa cagccaagaa atcatctctg atacaaaatt
     9841 gtcattagga gaaaatgttt ggggacagta ttttcatcga tctacttata tcgatcgcct
     9901 taaaaaagca cagccaagaa tcgaaacagc aaatatacat ttcaaaggga tgaatgaatt
     9961 cgatttggat gtaacagaat ataaagagat tgctttgatc gcaaaaaaca atcaatacta
    10021 cccagtgatt gaaaatggaa cagtattaga tgaaaaagta gcaaatccta caaaaaatct
    10081 ccctatcttg gaagagttca aagactctgc aaaaatcaaa gaattaacaa agcaatacaa
    10141 tcagttatca tcagaattac aaaaagcaat ttctgaaatc aagtacacgc caagagcaag
    10201 caataaaaat ctgatccaat tgaatatgaa tgacggcaat caagttattg taaatatcaa
    10261 taatcttgcg aaccaaatga aatattattc gcaagtagcc aaagatatgg atgaaaaagg
    10321 tgtgatcgac atggaagttg gtatcttctc gcatccttac ggtagcactc aagaaacgga
    10381 agaatcgggc caatcggaag aaacaactga aagtgaagaa aatgcggatc aaacaaacga
    10441 atcaagtgaa gaggcagaaa cgcaaaaagc acaaacacct ggatcgtcta gcacaaacgg
    10501 atcatctgat tcaaatgatt caagtgaaac aagtcaaata gaagataatc caagtacaac
    10561 tccgtcaagt ttaaatgaaa attgataaaa caactttaaa aagtaagaat gtactttcta
    10621 aaaaccagta atttatggta gaattggaag cagtgaatat atctattaat aagtatattt
    10681 taaaacggac gctagttgtt cgcttgtata tggaatagta agtaggaggg aatcccattc
    10741 atggcaaaaa caggaatgta tgtaggcctt gatattggaa cgacatctgt taaagttgtc
    10801 gtggctgaat atatcgacag ccaaatgaat attattggag taggaaacgc aaaatcagaa
    10861 gggattaacc gaggcattat cgttgatatc gataaaacgg tccaagcgat acaacgagca
    10921 gtacgacaag cagaagaaaa agcaggtatc cagatcaaag gcgtaagtgt cggtttgcca
    10981 gcaaacatgc ttgaagtaga aaattgccag ggaatgattg cagttaacgg agattcaaag
    11041 gaaatcaccg atgaagatgt cagaaatgtg gcttcagctg cccttgttcg ttcaatacct
    11101 cctgaacgtc aaatcgtgtc aatattgcca caagatttta ctgtcgatgg gttcgaggga
    11161 atcaaagatc ctcgtggaat gatcggcgta agattagaaa tgtacggtct gttgtttaca
    11221 ggacctaaaa cgatcatcca taacatacgt aaatgcgtgg aaaatgccgg attaatcgtc
    11281 aacgaaatgg tgattactcc tttagcgctg actgaatcaa ttctttctga cggagaaaaa
    11341 gattttggta cgatcgtcat tgatatgggt ggcggccaaa caacaacggc tgttatgcat
    11401 gataagcaat tgaagtttac caatcttgac caagaaggtg gcgaatttgt cactaaagat
    11461 atctctattg tattaaatac gtcatttaac aatgcagaag cgttgaaaat aaactacggg
    11521 gatgcatacc cagaacggac atcaccagat gaagaatttc cagtagatgt gattggtcag
    11581 tcagaaccag tgaaagtcga tgaacgctac ttgtcagaaa ttatttcagc tcgaatggaa
    11641 cagattttca acaaagcaaa agatgcattg gaccaaatcg aagcattaga gttgccaggt
    11701 ggcgtcgtat tgacaggcgg agctgctagt cttcctggtg ttgtggactt ggcacaagaa
    11761 atcttcggcg taaatgtaaa attgtatgtt ccaaatcaca tggggctgag aaacccggta
    11821 tttacaaatg taataagtat cgtggattat tcagcaaact tgagtgaagt gtatcaattg
    11881 gcaaaaattg ctgttacggg tgaaacctca gcagctcgtc aaatggtagt tgaacaagaa
    11941 acaacaaaca catatgagag ctatgaagca cctgaagaaa caatctacga tgagccggaa
    12001 ccaaaagagt ccggagaaaa tgtaaaaaat aaaatcaagg gtttctttac aaatattttt
    12061 gattaattca tcctgggagg aataagatat ggaattctca attgataata atatcaatga
    12121 cggcgcagta atcaaagtca tcggagtcgg tggtggcggt ggaaatgctg taaaccgaat
    12181 gattgaagaa aacgttaaag gtgttgaatt cattacagcc aacacagacg tgcaagcatt
    12241 aaaaaattca aaagctgaaa cagttatcca attaggcccc aaatatactc gtggtttagg
    12301 tgctggttct cagccagaag ttggacaaaa agcggcagaa gaaagtgaac aatccttacg
    12361 cgaagcttta gacggtgcag atatgatttt catcactgca ggaatgggtg gaggaaccgg
    12421 tacaggtgct gcaccaatcg ttgcaggtat cgcgaaagaa ttgggcgcat tgactgttgg
    12481 tgtcgtgaca cgtccattca cttttgaagg accgaaacgt ggccgttttg ctgcagaagg
    12541 tatcgctcgt ttgaaagaaa atgtggatac attattaatt atctcaaata accgtttatt
    12601 agaagttgta gataaaaaga caccgatgct tgaagctttc cgtgaagcag ataatgtttt
    12661 acgacaaggt gtacaaggta tctctgattt gatcacagcg ccaggttacg ttaaccttga
    12721 ctttgctgac gtgaaaacag tgatggaaaa ccaaggtaca gcattgatgg ggatcggtgt
    12781 ggctagtgga gaagatcgcg tagtagaagc aacgaaaaaa gcaatctcct ctccattgct
    12841 tgaaacatct attgatggag ctgaacaagt acttcttaac atcacaggcg gtttggatat
    12901 gacattgttc gaagcacaag atgcttcaga tatcgtagca aatgcagcaa ctggcgatgt
    12961 aaatatcatt ctaggtacat ctatcaatga agaaatgggc gatgaaatcc gtgtgacggt
    13021 catcgcaaca ggtatcgatg aatcaaaaaa ggaacgcaag tctagccgac cagctagaca
    13081 agcacaaatg caatcacctg ctcaaaagac tgtattagat atggatcaag ctaaaccaac
    13141 ttcttcagaa gaagaaaaca gttttggcga ttgggatatc agacgtgaac aaaatgttcg
    13201 tcctagagtt gatgattcca attttgacaa tatcgaaaaa aaagaatttg atacattcaa
    13261 ccgcgaagaa acaaaatctc atggcgatga tgaattaaat acgccaccgt ttttccgtcg
    13321 taaaaaataa aggagaggaa gagcacatga ttgctgacaa cttgcagaaa atcaagcaag
    13381 aaattcagga ttcgtgtgct cttgttcatc gtgatccgaa agaagttaca ttagtagctg
    13441 taacaaaatc tgttgaatcc gctcaagctg aagaattggt tagagctggc gttatgaatt
    13501 gtgcagaaaa ccgagtagat aaattattag aaaagaaaca agatttattt gattttcctg
    13561 aaataaagtg gcatttaatt ggtaatttac agcgcagaaa ggtaaaattg attgtaaatg
    13621 aaattgatta ctttcatgcg ttggacagtt taagattagc agaagaaatc cagaagcggt
    13681 tagagaaaga attatcctgt tttattgaag taaatgtttc tggtgaggaa tcaaaacatg
    13741 gaatttcacc agatgagctt ttaccgtttg tggaatcttt agctgcctgc acaaaaatca
    13801 aggttgttgg gttgatgact atggcgccta aagaagcttc tgatgagcaa atccataaag
    13861 tgtttggtac gctgaaagaa ttgcgtgatc ttgttcaagc aaaaggttat gcccatgcac
    13921 catgtagtga attaagtatg gggatgagtc aagactttca aattgcgatt gaagaaggtg
    13981 caacatttat ccgtgtagga acagcgctat tcaaagacga ataaaggagg aacaaaaatg
    14041 ccgcttttaa acagaggagc aattgctaat tttttcggat tagctgatga ggaagagtac
    14101 gaataccaag attatcctga aacaacaaaa aaggctgtac agccaactaa aaaaaataca
    14161 acacagaatc aatcaactca ttctgtatct gctgcgaata ctcatgaaaa acaaaaaacg
    14221 gcgacttcac gtcctgtaac gcatgaaagt attcagccgg ctgaaaagaa aaaagtaatt
    14281 gaactacatc aatcacctac aaaaactgta gcgaaagaaa gaacagcttc aagaacctct
    14341 acacaaccta aagcaaatct tgctggaaaa atcactgtaa tggaaccaag agcttattct
    14401 gaagctatgg cgattgctaa acgaatcatt gctggagaag caaccttagt gaattttcat
    14461 ttagtagaag aaaaacaagc gagaagaatc gttgattttc taacaggaac agtttatgca
    14521 ctagatggag atatcaaacg tgttggagat gagatttttc tttgtacacc gactgggatg
    14581 gagattgaca gttcaaccgc tcaatctttt gcagatgcga atttgtttga tctgtaagag
    14641 gaggaactac gatttatact attgcactat tacttcattt acttcaacgg gcagcctatt
    14701 tctatagcat tatcttgatg gtatatgcat tgctctcttg gtttccaggc gggtatcaat
    14761 cagggctagg acgattatta gctaagatct gcgaaccgta tatcagtttg ttcgatcgtc
    14821 tgcctttgag gattggaccc ttggatttca caattttagg ggcaatcctg atcttgaact
    14881 tagccgtaca aggtttagca cagatcgtta tatggataac tgtccattta gtttattgat
    14941 tgaagcaaca aaatctttat tctcagaata gataggaggg gaaaattttg gatgcaaatg
    15001 tataccagca ctttcgggca gatgaacgta catttatcga ttcggtaggc gattggattg
    15061 aacaggttca aggacaatat gcaccctatc tgaccgaatt tcttgaccca agacaatcct
    15121 atattttaga aacactgatt cgacaaagca gtgatttgcg tttcatgttt tatggcggat
    15181 atgaacaggc agaaagaaaa agatgtttga ttttccccga ttattacgaa cctgtagagg
    15241 aagattttga aacagcattg tatgaaatcc attatcctag caaatttgcc agcctaagtc
    15301 atgggaaaat ccttggaact ttagtgggaa cggggatcaa acgctcttac tttggcgata
    15361 tcatctcgga cggtgatcgc tggcaagtat ttattgcaaa tgaggtggat cattttgttg
    15421 ctgcacaagt aacaaagatt ggaaaggttg ctgtacgctt ggaaccaatc aaatacacag
    15481 aaatgattat gccgaaagat agctggacac aagaaagagg aacagttagt tccatgcgac
    15541 tagacagtgt gatctctgag atttataata tttccagaca gcggtccaag cagctgatcg
    15601 aagctggtaa agtaaaagtg aactggacag aaaatacaag acctgatttt gtacttgaac
    15661 tattagatat tatttcagtc agaggttatg gcagagtcca gatccaagaa cttgagggga
    15721 agacgaaaaa agaaaagtac agggtactgt tcggtgtatt acgtaaataa aataaaaagg
    15781 tggtttactc atggctttaa ctccattaga tatccaaaat aagacattcc cgacaaaaat
    15841 gcggggatat aatcaagacg aagtagatga tttcttagat ttagttgttc gtgactatga
    15901 ggaattgact caaagaaatc gagaattaga aaaagcagtc aaacattctg aagaaaaact
    15961 tgagtacttc aatgaattaa aagatgcttt gaaccaatca atcatcgtag ctcaagatac
    16021 ggctgataaa gtaaaaacaa gtgcaagcaa ggaatccgaa gtgatcgtta cttctgcaca
    16081 aaacaaagca gatgagttag ttgcgaacgc tgaaaaacgt gcacatcaat taacaactga
    16141 tgcagaagaa aaagcaagaa aaatcttgac agatgcaaca gaaaaagcaa gacagcttgc
    16201 gacagaaact gaagatctta agaaaaaaac acgagtattc catcaacgta tcagcttgat
    16261 gcttgagtct caattagaac aagtgaaaag tccggaatgg gatgaaattt tacaaccatt
    16321 ctccagctac gtgactgaca gccatgaagt gatcaaagaa gttttagcaa aacagcttga
    16381 caatgaaaat gatactgatg taaactcaga cataggagtc atagaagcac ctatgacggc
    16441 atttgataca caggctattg atatctcgat cgtgcctggt gaaaatgaaa acgagtagaa
    16501 cagaaaagca ctttgttatt cttaaagcga gccgatgaga gtggaagttc ggtaagacac
    16561 tgcatggtga aagatcctct acttgagacc tgcttttccg aaattagagt aagaagcagc
    16621 ggctgacctc gttattggtc acagagagtt tgcactagat agatgcaact aaatttgggt
    16681 ggtaccacgg catgttcgtc ccttgcggct gaacatgctt ttttgtgtcc ggaaaacaaa
    16741 taaaggagtg tatggttgaa tgaaaatgaa agaaaccttg caattaggaa aaacaaaatt
    16801 ccctatgcgt ggtaatttgc caaaccgaga aggcgagtgg caaaaagaat gggaagaaaa
    16861 tcgaatctac caaaaaagac aagagttgaa cgaaggaaaa cctagctttg ttcttcatga
    16921 tggacctcca tatgccaatg gtaatatcca tttaggacat tcattgaata aaatcagtaa
    16981 agacattatc attcgttcaa aatcaatgtc tggctatcgt gcaccatatg ttccaggatg
    17041 ggatacacat ggattgccga ttgaacaagt attggctaat aaaggcgtga aacgtaaaga
    17101 aatgtccatg gcagaatact tggaaaaatg tcgtgaatat gcactttccc aagtagataa
    17161 gcaacgagca gatttcaaac gcctaggagt tgcaggagat tgggaacatc cttatgttac
    17221 attagatcct tcttatgaag ctgcacaagt acgcgtattt ggtaaaatgg ctgaaaaaag
    17281 ctatatttac aaaggattga aaccaattta ctggtctccg tcaagtgagt cttctttagc
    17341 ggaagctgaa attgaatata aagatgtaaa atctccatct atttttgtag ctttcaatgt
    17401 caaagacggc aaagatattc ttgatgaaga tacagctttt gtcatttgga cgacaacacc
    17461 atggacattg ccagcaaacc aaggtattgc tgtaaatcca aaatttacgt atgtattggt
    17521 ggaagccgat ggccgtaagt ttgtcgtagc gaaagatttg ctagaaacag tccaatcaga
    17581 aatcggttgg gaaaatgtaa cagttctaaa agaatttgct ggacaagaaa tggaatatat
    17641 gactgcaagt catccattct atgaacgtga atcacttgtg attttaggcg atcatgttac
    17701 tttagacgct ggtactggat tagtccatac tgctccagga catggggaag atgactatat
    17761 cgctggtagc aaatataaac tatctgtagt gtcacctgta gactcaaaag gattgtttac
    17821 agaagaagca cctggatttg aaggtatctt ttatgacaaa gcaaatccta tgatcactga
    17881 tctattaaaa gaaaaaggag ctttgctgaa gctagatttc tttactcata gttatcctca
    17941 cgattggcgg acgaaaaagc ctgtgatcta tcgggcaact ccacaatggt ttgcatctat
    18001 cgataaattc cgtcagaata ttttggatga agtagaaaaa gtagagtggg ttatcccatg
    18061 gggtaaaaca cgtttgtata atatgatccg tgaccgcggc gattgggtaa tttcccgtca
    18121 gcgtgcatgg ggagtaccac ttccgatttt ctatgctgaa aatggagaag caatcatcac
    18181 accagaaaca atcgaacatg tggcaaaatt atttgctgaa catggatcaa ttatctggtt
    18241 tgaaaaagaa gcgaaagaac ttcttcctga aggatttacc cacccaggtt ctcctaatgg
    18301 cgaatttaca aaagaaaaag atatcatgga tgtctggttt gattctggtt cttctcatga
    18361 agctgtttta cgtcagcgac cagaattatc attcccagca gatatgtatt tagaaggatc
    18421 agaccaatat cgtggctggt tcaactcaag tatcacgact agtgtggcta tcaatggtgt
    18481 agcaccatac aaatcagtta tttctcaagg atttgcttta gatggtgaag gccgtaagat
    18541 gagtaaatct ttaggaaaca tcattgcacc agaaaaagtg atcaaacaat ttggtgcaga
    18601 tatccttcgc ttatgggtat ctagtgtaga ttatgaagca gatgttcgtg tttctatgga
    18661 tatcttaagt caagtggcag aagtatatcg taaaatcaga aatacgatgc gtttcttatt
    18721 agcgaataca gaagatttcg atcctaaaaa agatactgtt tcgtacaatg atttacgttc
    18781 tgtagacaaa tatatgaccg ttcgattgaa ccaagtaatc aaagaaatcc gagaagaagg
    18841 gtacgacaaa tataacttca tgcatattta ccgtacagtt atgaacttct tgacggttga
    18901 tctatcgtca ttctacctgg attttgctaa agatgtagtt tatattgaag cagaagatga
    18961 ttatcaacgt cgttgcatgc aaactgtttt ctatcaaaca gctgtagctt tgacaaaatt
    19021 attgactcct atcattccgc atacagcaga agaaatctgg tcattcctga aagaagaaga
    19081 ggagtacgtt cagttaagcg agtttcctgg ttatgaagag tttgcaaacc aagaagaatt
    19141 gatggatacc tggactgctt tcatggactt ccgtgataat gtcttaaaag cattggaaga
    19201 agcaagaaac agcaaattga ttggtaaatc tttggaagca aaactaactg tttatccaaa
    19261 acaacaagtt cgtgaaatgt taaaagcttt ggatgcagat attgcacaat tattgatcgt
    19321 ttcaccagac tactttgaag ttatggcagc agatgaacaa gtgccagata acgctatggt
    19381 atttgatgat gttgctatta cagtggaaaa agcagacggg gaaacttgcg atcgctgtcg
    19441 ccaagttcgt aaagatgtag gagtagatga aaaacttcct catctatgcg gtcgttgtgc
    19501 aaaaatcgtt gaagacttct atcctgaagc tgtagctgaa ggattcgaag aaaagtaaaa
    19561 gggaaaaggg ggtgtgacat gttctttgtc acaccccctt ttgttatcaa tcgtttaatt
    19621 ttccacgttc acgataccag ttatctggtt gccagatcca ttcgaatccg tctttttcaa
    19681 gtagttcgaa agctgcttta ggtcccattg atccagcttt gtagttggga aaatccggtt
    19741 gtgtttggtc ccaagctttt cgaatgacat ccacgatatg ccaagattga gcgacttctt
    19801 cccaatgaga gaagttcgtt ccatcaccat tcaacgcatc taatagtaat ttttcgtaag
    19861 cttctgggct attttcaacc atttctgcac tatttctaaa ttccaatttg attggttccg
    19921 tttcaaatcc ttgccctact tctttcccat tcaaggataa agaaaatcct tcagttggct
    19981 gaatatagat cgttagaaca ttaggtggaa gtgtcgtatc gtcacatggt tcatcgacag
    20041 atgttttgaa tacattaaca ggaacttgtt tgaatacgat gttgatccga gtcccttttt
    20101 cagtcaaacg tttgcctgta cgtacataga aaggaacacc tgaccaacgg aagttatcga
    20161 tagtgaattt gcctgcgacg aatgtttcag tctctgattg agcatccact ttgtcttctt
    20221 cgcgatagcc cttgaattgt acaccatcca attcaccttg agcatattgc ccgcgaacga
    20281 agttttctaa cacttcttct tcgttataaa tgcggatagc tttcaatgct ttgatctttt
    20341 ctgtacggat ttctttttcg gaaaatgcta ctggcggttc cattgcaagt aatgctacaa
    20401 cctgtaaaat atgattttgc accatgtctt taagtgcgcc actatgatcg tagtagcccc
    20461 cgcgatcttc tacaccgagg ttttcgccaa aagtaatttg gatattgtcg atatagcgat
    20521 tgttccattg agcctcaaaa atattattgg caaaacggat agctgagatg ttttggatca
    20581 tttcttttcc taaataatga tcaatcctga aaatatcttg ttctgggaat acttctctga
    20641 tttgctggtt caattcaaaa gcagactggt aatcagaacc gaacggtttt tctatgatca
    20701 aacgattgaa gccatttttt gtcataatat tttgtgattt caaatgagaa acgatcgttc
    20761 caaaaaattg aggtgccata gccaaataat agacacggtt accgtttaat tgatagtgtc
    20821 catctaatcg gtcagacaat tcttttaatg tctgataatg cgttgaatca tttacatcat
    20881 gggattgata gtaaaaatgg cttgcaaagg tagttgcttc ttcatctgaa ggattcaatg
    20941 cacggattgt ttctttgaca acttcacggt agtgctcatc gctccaggga cggcgagcgg
    21001 ttccgattac agcaaaatgc tcgcctaaat ttccttttct gtaaaggcgg aacaaagacg
    21061 ggtacaattt acgctgggct aggtcacctg taccgccgaa tatcgtaaac aatacatttt
    21121 tttcattaga catgtggata cactccttta tattttatcg acaaaaatcg tgctggccgc
    21181 cttaaagctg acggggattt tctttctatc attttggata gtgatcggtc cttcataagc
    21241 tgcgatgtca acgatctcat attcttcgtt gatattcagg tcaatagtga ctaagtaatc
    21301 caacaattct ttttcatcaa gcactcgtgc gatacgcact tttgtaccga caggatagga
    21361 ctccaatgtt tgtcggcgtt cttcatggac aacttcctct tttttaggaa tcgatcctcc
    21421 atgaggacaa tgttcaggat gattcaaata gttttctaaa tgatctgcaa gatgttccga
    21481 agtcacatgt tccaatactt cagcatcatc atggacttca ttccagctat agtctaaatg
    21541 ttcaactaaa aaaacttccc ataagcgatg tttgcgaatc agactgctag ctttttgcag
    21601 tccgcttgca gtcaattgta caccttgata aggggaatgt tctacaagct cttctttcac
    21661 aagtttagaa atcatttcac tgacagatgc agctgagaca tctagactag atacgatttg
    21721 tttattgttg actttcgttg tatctccacc tagttctaaa atgattttca aataatcttc
    21781 tcgatttggt gtcatattcg atcatccttt catggtaaaa aaccatcatt agtaagtgta
    21841 acaaaaaaaa cgttaattga ctatttttct aaataaaaaa caaaggattt ttagacgttt
    21901 ttacaacaaa aaacgaaaga tgatagagaa gactcattct ttcgtttata aaggatatat
    21961 atatacattg ggagggatct atttttcaga taacaactta tatgaagtgt ctgcagattc
    22021 gtcataaaga gtttgttcgg gataagaaaa gataagataa aaaccatact gatcacgtac
    22081 gactaaacga tattcatcta tttctaccac agatgtgcca agagactgac cgttgatcaa
    22141 aaacgtgtaa tcagatgtaa tctgtaattg ttgtttttgc tggctatcac taaagaacca
    22201 tgtaccttga tactttgaga gtccttctga cttttccgct tgttttttct ttttcaagta
    22261 aataaaactt gagagaacgg taatgagtga accaagaaaa tagatcattt tttttatcac
    22321 caaatcactc ctttatgaga aaataatcgc atagacatat tctttcttct ttatttcatt
    22381 gtaccgaata aaagctaaaa tttctctaag actttcgact ggttttttta ctgtttttct
    22441 acaaattatg ataggataca tagtaggaag aaaggtgaca taatttgaag tatcagcaag
    22501 tggaaatagc tttttttatt gaccgcccga atcggtttat agcacattgt atgaaaaaag
    22561 acggagaaat aattattgcg catgtcaaaa atacaggtag aggaaaagag gtcctgatac
    22621 caggcgctga agtggctatt gtatttgctc ctggacctaa acggaaaacc gcctatgatc
    22681 tgattgctgt aaaaaagaag ggtgactggt ttaatatcga tagtcaattg cctaatcggc
    22741 tggcaattga tggtatttta gacggcacga tccttttacc aggattaact ggtccattta
    22801 cggtatacaa gcgtgaagta acttatcttc actccaaatt tgatatttat ttagagacgg
    22861 tggtcggaca aaaagcattt gttgaagtca aaggtatgac acttgaaaat aaagcgattg
    22921 gtgcttttcc tgatgcgccc acactcagag gattgaaaca tataggagaa ttaacgtatg
    22981 ccgcacaaga tggttatgcc gcttatgtct tgtttattgc tcagttcgaa catcttcatc
    23041 tggcaacgat ccatgaagaa atgcaaccag cactagctga tatggttcga catgcgcagc
    23101 aaagcggagt tcaaatcctc gcctataatt gtcaagtatc ggcagatgag gtaactgtca
    23161 agcaggccat tccatttgat gtagatatcc cttttgaaga tcctatacaa tagaaagaag
    23221 gtaaaataat gatcaactta ggtattatcg gaacaaattg gattactcat caatttgtcc
    23281 aagcagcttt aagcacaaaa aaatatgaat tgacagcggt atattctaga catttggaaa
    23341 aagcacagga atttggttct cattatgaag gggatatcga attcgcaaca gatttgaaga
    23401 cttttttcaa tattactcat atggacacca tttatattgc ttctccgaat agtcttcact
    23461 ttagtcaagc taagcaagca attttggcag gaaaaaatgt gatagttgaa aagccagctt
    23521 tttcgacgcc aaaagaaatg gatgaaatca tccatttggc aaatgaacac aatgtgtatt
    23581 tttttgaagc agcaagaaat atccatgaat caggttttaa aaagattgcc gattacttac
    23641 cattaaaaga cgagataatc ggtgcaaatt tcagctatat gaaatattct tcgcgctatg
    23701 accaagtatt agatggagaa gaaccgaata tcttctcgac tcatttctca ggtggtgcat
    23761 tgatggattt aggtgtttac cttgtatatg cagctgttgc ttggtttgga atgccaaaag
    23821 aagtccatta tttcccaaga aagatttcta ctggtgtaga tggtcttgga tgggggatct
    23881 tacgttatga gaattttgat gtatcgatcc agccaggtaa aatcggtgat tcttatttac
    23941 cgtcagagat ttattttgac tcaggaacgc tagtgatgaa tggagtcaat gcaattgaac
    24001 aagcacaatt tcatgatcgc gcgcataaag aaatcattga tttggcggta acagctgcag
    24061 aaaatccaat ggcagatgaa gcagaggatt ttgccaatgt catggctcat ccgaaagatc
    24121 cagcttgggg attgttgtat gaagagtgga tcgagctagc tagaaatgtg aaccaagtga
    24181 tttatgattt aagaaaaaat ggcggcatca aattcgatgc agataatgag gaagattttt
    24241 aatgaacgaa tttgtacaag tatttcccga aaattggcag aaaaaatatg aggataaagg
    24301 gtttatctca ccttctctta ttcaacgagc agtttttcag ccactaagta ataaacaaag
    24361 tatagtagcg gtttctccta caggttcagg taaaacactg gcttatcttt ggcctctact
    24421 actaaacgtg gaaccaggtg aagcaagtgc gttggtcatt tttgcttctt ctcaagaact
    24481 agctattcaa gtagcagatg ttgcacgtga atggggaaaa gacaaagagt tgaaagtcca
    24541 atctttggtg ggcggagcca acgtcaaacg tcagattgag ggtttgaagg aaaaaccaga
    24601 aatcttgatt ggtacaccag gaagaatatt ggaattgatg aaagcaaaaa aaataaaagc
    24661 acatcaagtg aaaacaatgg tatttgatga agcagaccag ctatttgacg ctgggaacag
    24721 ccaaatcatt gatcaaattc ttcatcaagc tccaacagag tatcagctag cttttttttc
    24781 agcgacagca gatcgttccc ttgaaagcat tgaaaaaatc actgggaaaa cgtttgagac
    24841 aattgacgtg acagcagagg acgattctcg aaaaggactg agacattact ttttacgtgt
    24901 accgatacga aagaaagatg actacttgag acgtctcacg catatagaag attttcatgg
    24961 tctgattttc tttaatcaac tgactgaatt aggcgtaatg gaagaaaaat tgctgtatag
    25021 aggtgtaccg gtaggaagtc tggcctctga tcagaacaag ctactccgaa aagcagcgct
    25081 agaacaattc aaaaagaaaa tggtcagagc acttttgacc acagatgtgg cggctagagg
    25141 tttagatatt acaggtcttc cttatgtagt gaatgcagaa gttccgttaa gtgaagaagc
    25201 ttatttgcat cgttcgggaa gaactggtcg aatgggtaat gaaggaatag taattacact
    25261 cgtacaagat aataatctaa gagatttaaa acggctttta cgtccatcaa atataacact
    25321 tgaagagatt tttttatacg gaggtaagct gcaaactgag cagccaacca aagaagatgc
    25381 agctcctgca gtaaataaaa agtatgcgaa aactttttct ggaaaggagc agaacccaga
    25441 atataacaaa gaaaaaataa gaaaaaaagt taaaaataaa tcaaaaaaag agagaaataa
    25501 aggagcaagg cgcaaataag cgccttttta tgctatgatt atgaaaacgg gaacaaaatt
    25561 ggttgccaga ggaggggtta tgtgaaagaa cgtcaagaag agatcttgca gctattgaca
    25621 gaaaaccaac aaggattgac agcaagtgat gtagctgagc gcttaactat cgatcgaagc
    25681 aatgcaagcc gatatttaag tgaactatac aaggcccatc atattgtaaa aaccgctggc
    25741 cgaccagtcg tttactcttt gccaacggaa aagtcaaaat ctgatgaagt gcatgtcgac
    25801 tcttctacgc aagtgacttt tgaaacactc gttggagaaa atgattcttt aaaagtcagc
    25861 atacagcagg caaaagctgc gattctttat ccaccgcggg ggttgcatac gattattttt
    25921 ggagaaactg gtacagggaa gtcgatgttt gctgaatgta tgtatcattt tgcaatcgat
    25981 tcagagatgc tttctgcaga tgcgccattt gtttctttca attgtgcgga ttatgctcaa
    26041 aatccgcaac tgctctttgg acacatcttt gggatcaaaa aaggggctta cacaggagca
    26101 gctcaagata gtcccggtct aatagccaaa gcagacggcg gtatattgtt tttagatgag
    26161 atccaccgtt taccgccgga agggcaggaa atgctgttta ctttcattga taaaggtatt
    26221 taccgtccgc ttggagaaag tgcacaagta catgaagcat ctgttcagat cattggggcg
    26281 acgactgaat cttcagaaag tttcttatct acgttcaatc gacggattcc tatggcaatc
    26341 accttgccga atcttgcttc acgttcactg gatgagcgtt atgaaatcat ctctcttttt
    26401 atcaaacaag aagcgaatcg tttgaatcaa cgtatcgata tcgaaaaaga agcgattctt
    26461 gccttcatgc tttatgatgc ggaaggaaat atcggtcaag tgaaacgaga cttgaaacta
    26521 gtctgtgcaa aatctttttt acattaccgt actcatcatg agaaaaagct aatcattcga
    26581 aaagaagaac tttcccttca agtacaaaaa ggattactga aaatcaagga aataccggaa
    26641 cgtctcgacc gattcatgga cagcaaaagt cagtatttga catttgaacc aggatttgct
    26701 gatgttgtct ggtcacaaga tccagaacga aacatgcagg tctataacga tattgaggaa
    26761 aaagtcactt ctctttccga aaccggtgtg gagaacattg atctagagac cctcatttca
    26821 aaagatgtgg atgcttattt ccagacatat gtcaaagaat tgactaaaag ttcgattccc
    26881 aaagatcttt tgccagatga aatctggcgg ttaaccaatc aattgtatga tgtggctgaa
    26941 aaacggttag accgtattta taatgaaaaa gctcgttttg cttttgcttt gcatctgcaa
    27001 agtacattgg atcgtgtgag agaaggtcat atgatcgtcc acccagattt gaataacgta
    27061 cggaaaaatt ttaagaggga attccaggta gcaatcgatc tttcaacgat cattgaagaa
    27121 gaagaacaag ttgagattcc ttttgatgaa atcggcttta ttagtatgtt tctttctatc
    27181 aatctaggag aaagtgagcc tgttaaagaa aacaaagtag atgttctcat attgatgcac
    27241 ggcagacaga ctgcttcaag catgttggag atggcacaag aactattagg aacaaaagta
    27301 gggaaagctt ttaatatgcc actggttatg gaagttcaaa caatgtatga agaagtgtta
    27361 caatatgtca aaaactatca agagcatttg acgaatggcc tactcttgtt aacagatatg
    27421 ggatcattaa acaccttcag tgatttgatt tatgaagaga ctggcattcg gacgaaagct
    27481 atcacgatga caagtaccat gattgtgata gaagcgatac ggatggcaag tgttggaaga
    27541 agcttagaag atatttatca aaatatccag ctttcctttg aatcgatcgt acgtgatcag
    27601 ttcaaagcgg aaaagtctga aactttacag cgtaaaaaag ccgtaatcgt gacatgcttt
    27661 actggtgaag gagtagctgc caaattatat cagcgtatta tgccagtgat taatcaaaat
    27721 aaggtagaac tgatccagat gcagttttta gaacgtgaat cattcaaaaa acacatcgat
    27781 gccttattgg aagaatatga gatcaaagca attgcgggaa cagttgatgt tgattatcaa
    27841 aatatcccat ttttttcagc ctatgacgtt tttgatgatg aaaaactaaa tattttaaag
    27901 cgtatagctg atgatgagat cccgacagct aaaattgtcg attcacttgc tggatcatta
    27961 agcgatgtct tttctattga tgaactgatc cgttcattgc aaaagacagt tcagcagatc
    28021 cagaccgatt tgtcgattat cgtggaacct ggagtagata ctggaatcgt tatccattta
    28081 gccttcatgt tagacggatt gatcaaacaa gaaagccatc ggcaatttga gaaactgtct
    28141 attttctcaa aaatttatcg tttggaaatg gatatcgttc gggcaaactt gatggcacta
    28201 gaacgaaaat atcaggtaaa aattccagaa gatgatatcg catttcttac acaaatgttt
    28261 cttgaaaata aaataaaaaa atgaacacac taatcatact gtttacacac taaacatctg
    28321 tgtgcgcact ataaaaaaga gctaaaaagc atgaaaacgc tatttcagct ctttttttta
    28381 ttggcatgct tcttgcgtta tataaaagtg taaaaaaata aaaggagtgt taaaaatggc
    28441 aaaaaaaaca atcatgttag tttgttcagc agggatgagt acgagtttat tggtcacaaa
    28501 aatgcaaaaa gcagcagaag aaaaagggat ggaagccgat atttttgcgg tctctgcctc
    28561 agatgcagat aataatttag aatctaagaa tgtcgatgta ttattattag gtcctcaagt
    28621 acgtttcatg aaagctcaat ttgaacaaaa acttgcgcct aaaggtattc cattagatgt
    28681 catcaatatg caagactatg gcatgatgaa cggagaaaaa gttcttgcac aagcagaaaa
    28741 tctaatgaaa taaatcaagt atgcagttgg gggtataaca acgtggaaga tcaacaaaac
    28801 ctagaagcca tcatggggtt aatcatgttt ggcggtaatg caaagagcga tgcaatggaa
    28861 gcgattgcgg ctgctaaaat cggtgatttt gagttagcgg ataggaaaat cgctgatgca
    28921 gaagaatcac ttatacaagc gcatcatgca caaacagaca tgttgacaca agaagcacaa
    28981 ggaaatcata tgcaagtgac attattgact gttcacagtc aagatcactt aatgacatct
    29041 attgcgttta ctgatttagc aaaagaaatc atcgaacttt atcgtcgcgt atacagcgaa
    29101 taaatacaac ttcttttttg aacggaagaa gaaaaatttt tgaacggatt ttttattttg
    29161 ttataaggag aactctcttt atataaaaaa cttataggag gaagtaaaaa atgaacggac
    29221 tatcaagttg gatggagaaa tacatcctgc cggttgccgc aaaaatcgga gcgcaaaaac
    29281 atttagttgc tttgcgtgat gcttttatcg gcacactgcc cgcaacaatg gcgggatcag
    29341 tcgctgtaat gatcaatgca attattcgtg atttaccgca acagtttaac agtacctatg
    29401 ctgacagttt agcagcagac aaaggaatct ttgccgtatt gaatgtcatc atcggaatca
    29461 atggttttgt ctggaacggt acactagcaa tcgccggatt gatctttgcc ttttcttggg
    29521 ggtataatct tgcccgagct tatggagtaa atgatctagc tggggggatc gttggtttag
    29581 ctaccttgat tcaaggtatt tcctttggtc cagcattatc agcagcatta gatgtgaatg
    29641 ttccttcaaa tgtcgcagat gcaatcaata gttctgagat tggtgcaacg attgcagatg
    29701 gcacactgtc agttggatta tggggttggc taaaactaga tcatttgaat ggaaacgctt
    29761 attttacagt tatgatcatg ggagctattt cagtcattat tttttgtaaa ttgatgcttg
    29821 ccaacattac gatcaaaatg cctgattcag taccaccagc agtatctaaa gcatttgcag
    29881 ccattttacc agcgacgatc gctttatacg tcattgctat tatcaattat atcgtaggtc
    29941 gtgtagctga tgggcaactg atcattgatt tggtgcaaaa atatattgca gaaccattcc
    30001 taggattatc tcaagggttg ggagcagtat tgatcgtcac agtatttgtt caagtattct
    30061 ggttctttgg tattcatgga ccaaatgtat tggcaccagt gctagaggga atctggggac
    30121 aagcacaact aatcaatatt gatattttcc aaaaaggtta taacggaaaa actggtacac
    30181 cagccgtgtt agatgcaatc gaagatggaa aagcttatat gtgggtccgc ggatcgttcg
    30241 atgcttttgc atggtttggc ggatcaggcg gaacgatcgt cttactgata gctatcttga
    30301 ttttctctaa acgtgcagat tatctaacag tagctaaatt atccattggg cctggtatct
    30361 tcaatatcaa cgaaccagtg atgtttggtc tgccaatcgt attgaatgca atcatgttta
    30421 tcccattctt gattgctcca gtagtagcaa cagcaattgg atggacagct acttatcttg
    30481 gcctagtcgc tccggtatcg cagcaagtca catgggtagt accgccgttc ttattgtcat
    30541 tcttagcaac aggagcggat tggcgggcac cgatcgtaac attagtatgt atggtcgtga
    30601 ctttcttgat ttgggcacca tttgtcatct ctgcaaacaa aatggaccct gccgcaggcg
    30661 aacaacaata agatgaacgt gaggagcggg acgctttaag tttcgttcct cataatgaat
    30721 tttcgaggag gaactgaaat gattacaatt gccagtgcaa tgcaacaaga tgaaatcaaa
    30781 gaattgttag aaggatacaa tgaagaagga atgactttta catttaaaga aaaacaagga
    30841 atcaagctgt tctttgaaac aagtgcagaa gatccggaag ttgcggctaa aaaagcaaag
    30901 gaactaatca aagcacagcc ttggggaaca gtcctttatt ttcaatcaac agctgtttag
    30961 aaaatggttt aaaaagatag gatggttgag atgataaaaa acggtttatt tttatgtagt
    31021 attggttctt tgatcgtgtt gtatagtttg aatccacatt cgcaaaggta tgatttgtat
    31081 agtatggtca caggtctttt tctaacagga ttaggaatct atttcttcat gaaggggaaa
    31141 aaaagagaag aagcaaagga gaaaaataac taatggaaat cacgaaaaaa ttaaatattc
    31201 cagcatcgtt tttttacgat aaagttatgg actctgtcct tttcgatgta cgtaaagcga
    31261 ccggcaaatc agtaactaga aaacagctga aaaatttgga gtacgtcaaa caattctcgc
    31321 aaaataaccg ggctcgcata aaaatcgaag aagtcattga aaaccagtct tataagtttc
    31381 gtacatcgac aacgaagaat gaattcgtcg ttcattatca aatcaaacca ttggacgatt
    31441 cgacctgtga ggttcattac acagaaaaaa tggagtctta tggcatgctt caaaagttga
    31501 atgatatgct tttaggaaca atcttgatgt attttaaacg gagacgtttc aaaaaaatgc
    31561 tagatatgat cgaagcaacg tattaaatca atgaaacatg gtactattta gttattgaaa
    31621 aatgaagtaa gctagaagga atttttcata ggaagataga tctttcctgc gaatcctcac
    31681 tagctttttt gtaggaaggg gcagcaaaat gacaaacaaa gtaagagagc taggtgtatc
    31741 ttcgattgag gaaatgtttg atttagccac ttatgcattt aatggggcag atacacctga
    31801 aaggcgagaa cgttttcgta cacttgcgga gaattcttgg aattacggat tttttgatga
    31861 ggaggatcgg ctaacaagtc aagtcatggc gacccctttt ccagtgaatt tttacggaca
    31921 agagtaccta atggccggca ttggctatgt tgcttcttat ccagaagcaa ggggacaagg
    31981 cgggatcaat cagatcatgg aaaagatcct tgaagattgc cgtgatcgta aagtaagctt
    32041 atcttatctg gcgccattct cctatccctt ctatcggcgc tatggctatg aacaatcatt
    32101 tgataaaatc agttatcaac taaacagtcg tgatgtgccg tttatcaaaa aaaaatctgg
    32161 aaaaataaag cgtatggatt ttgaagatgc aaaagctgca atcagaagaa tttatgaaca
    32221 gatgccggaa aaccaacgag ctggtctgaa gcgcgaagac tggtggtata cgtataaatt
    32281 caagcaaaaa ggcaattacc agtttgcttt gcattttgat gataatgagg aagcaacggg
    32341 atatatcgtt tatcagcttg caacgtcttc ctttattatt gcggagtggg gatttttgga
    32401 tcatgatgct ttttgcagcc ttgttcgctt cgtaagttct cacaatggtg cttttgaaac
    32461 atttgaatat tcatgcggct acggcggtaa tgatcaaaat tatctattag aaaatccatt
    32521 tgcttctgta atgatcacac cttatatgat ggcgaggatc gtagatattt ctttgttttt
    32581 ggaaaattat ccatttaaaa agagacaagc tgcatttgcc ctacaaatag aagaagatca
    32641 ttatgcgaaa tggaatgaag gaatcgtgga agtaacaatc gaaaatggaa aaaatcatct
    32701 ctcaaaagta gaacaaacaa aactcccaat cgttcgagga agtatccaac atatcacgca
    32761 gctattatta gggtatcgta aagtggaaga gttggtattc caagaaagaa tacaggtgac
    32821 aaaagaatat gaagaaacgt taagctcaat cttgccacag caacagccaa ttcttaatga
    32881 ctatttttga taaaaaacgg gtgatgacaa tatttttgtc accacccttt tgtttttcat
    32941 ttggcatttg aaggaaaatg ttaaagtaaa tcctcttttt tcgtaaattt gaattttttg
    33001 ttttcttttg ttttagggtt ttcttttttg tggtcttgga ttgcttcttt cgtaatcttt
    33061 tcccgatttt ttttagatga atctaagcgt tctgcgtata ctttttcttt tggcttatca
    33121 ttcattggaa aggacctccc tacattattt cattctacct ctagtttatc atctgttaag
    33181 gactagtgga aacgttttgc ctaacttata agctgatttt tttagcagat aagcttgcaa
    33241 gtaaaaaaca aacaagctac aattgattta ttgaaattgt tcctgtagct cagtaggata
    33301 gagcaatcgc ctcctaagcg ataggtcgct ggttcaattc cagtcaggaa cgtataaata
    33361 acccctgtat cctttgcggt acaggggttt gttttgtatt tgtgccaaaa atgtgccaaa
    33421 ctagttatca tttttatata aactatcgaa cacagtattg attttttctt tatcctgatc
    33481 ttcaagctct cgaacaatat gaa
//
LOCUS       DAXLPI010000017        33160 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXLPI010000017 DAXLPI010000000
VERSION     DAXLPI010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.017"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(34..870)
                     /gene="pheA"
                     /locus_tag="ACF3T5_000723"
     CDS             complement(34..870)
                     /gene="pheA"
                     /locus_tag="ACF3T5_000723"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFD3631355.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(867..1376)
                     /locus_tag="ACF3T5_000724"
     CDS             complement(867..1376)
                     /locus_tag="ACF3T5_000724"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFD3631356.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(1369..2664)
                     /gene="aroA"
                     /locus_tag="ACF3T5_000725"
     CDS             complement(1369..2664)
                     /gene="aroA"
                     /locus_tag="ACF3T5_000725"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFD3631357.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREIGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(2677..3768)
                     /locus_tag="ACF3T5_000726"
     CDS             complement(2677..3768)
                     /locus_tag="ACF3T5_000726"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFD3631358.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(3785..4951)
                     /gene="aroC"
                     /locus_tag="ACF3T5_000727"
     CDS             complement(3785..4951)
                     /gene="aroC"
                     /locus_tag="ACF3T5_000727"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFD3631359.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(4953..6017)
                     /gene="aroB"
                     /locus_tag="ACF3T5_000728"
     CDS             complement(4953..6017)
                     /gene="aroB"
                     /locus_tag="ACF3T5_000728"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFD3631360.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(6050..7072)
                     /gene="aroF"
                     /locus_tag="ACF3T5_000729"
     CDS             complement(6050..7072)
                     /gene="aroF"
                     /locus_tag="ACF3T5_000729"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFD3631361.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(7731..8705)
                     /locus_tag="ACF3T5_000730"
     CDS             complement(7731..8705)
                     /locus_tag="ACF3T5_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFD3631362.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(8686..10569)
                     /locus_tag="ACF3T5_000731"
     CDS             complement(8686..10569)
                     /locus_tag="ACF3T5_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFD3631363.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(10587..12812)
                     /locus_tag="ACF3T5_000732"
     CDS             complement(10587..12812)
                     /locus_tag="ACF3T5_000732"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFD3631364.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            12927..13961
                     /locus_tag="ACF3T5_000733"
     CDS             12927..13961
                     /locus_tag="ACF3T5_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631365.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(14028..15041)
                     /locus_tag="ACF3T5_000734"
     CDS             complement(14028..15041)
                     /locus_tag="ACF3T5_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD3631366.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKHLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(15119..15307)
                     /locus_tag="ACF3T5_000735"
     CDS             complement(15119..15307)
                     /locus_tag="ACF3T5_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFD3631367.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(15747..15875)
                     /locus_tag="ACF3T5_000736"
     CDS             complement(15747..15875)
                     /locus_tag="ACF3T5_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFD3631368.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(15878..17251)
                     /gene="rlmD"
                     /locus_tag="ACF3T5_000737"
     CDS             complement(15878..17251)
                     /gene="rlmD"
                     /locus_tag="ACF3T5_000737"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFD3631369.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(17362..18417)
                     /locus_tag="ACF3T5_000738"
     CDS             complement(17362..18417)
                     /locus_tag="ACF3T5_000738"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFD3631370.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHADDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(18427..19857)
                     /gene="gatB"
                     /locus_tag="ACF3T5_000739"
     CDS             complement(18427..19857)
                     /gene="gatB"
                     /locus_tag="ACF3T5_000739"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFD3631371.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(19854..21326)
                     /gene="gatA"
                     /locus_tag="ACF3T5_000740"
     CDS             complement(19854..21326)
                     /gene="gatA"
                     /locus_tag="ACF3T5_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFD3631372.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(21326..21631)
                     /gene="gatC"
                     /locus_tag="ACF3T5_000741"
     CDS             complement(21326..21631)
                     /gene="gatC"
                     /locus_tag="ACF3T5_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFD3631373.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(21737..23773)
                     /gene="ligA"
                     /locus_tag="ACF3T5_000742"
     CDS             complement(21737..23773)
                     /gene="ligA"
                     /locus_tag="ACF3T5_000742"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFD3631374.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(23790..26024)
                     /gene="pcrA"
                     /locus_tag="ACF3T5_000743"
     CDS             complement(23790..26024)
                     /gene="pcrA"
                     /locus_tag="ACF3T5_000743"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFD3631375.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            26179..27738
                     /locus_tag="ACF3T5_000744"
     CDS             26179..27738
                     /locus_tag="ACF3T5_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFD3631376.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(27848..29779)
                     /locus_tag="ACF3T5_000745"
     CDS             complement(27848..29779)
                     /locus_tag="ACF3T5_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFD3631377.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(29797..30711)
                     /gene="pfkB"
                     /locus_tag="ACF3T5_000746"
     CDS             complement(29797..30711)
                     /gene="pfkB"
                     /locus_tag="ACF3T5_000746"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFD3631378.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(30708..31460)
                     /locus_tag="ACF3T5_000747"
     CDS             complement(30708..31460)
                     /locus_tag="ACF3T5_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFD3631379.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(31621..31830)
                     /locus_tag="ACF3T5_000748"
     CDS             complement(31621..31830)
                     /locus_tag="ACF3T5_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631380.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(31841..>33160)
                     /locus_tag="ACF3T5_000749"
     CDS             complement(31841..>33160)
                     /locus_tag="ACF3T5_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631381.1"
                     /translation="EDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSFTQQ
                     ADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKEKQF
                     LIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQNGEV
                     VVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENEVIC
                     IQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQSVQ
                     YQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMMCYK
                     VIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKEIKH
                     RLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
BASE COUNT         8806 a         7241 c         6071 g        11042 t
ORIGIN      
        1 taagttaaat aaatcaatca gtattcttga ctttcatcta cgcgtctctt ctactgtcag
       61 cactggataa cagcccaata tttgactttc tcctccgagc atcttgattt cttggagcgc
      121 attctctact aatgtcattg gacggtccaa caacaaatcg atcacgaaaa aatattctcc
      181 caagctagtt ttcaatggtc gggattcaat cttacttaag ttgatttccc gccagccaaa
      241 agctgccagc attttatgaa gcatccctgg ccgattagca ggtagtgtca agatgacact
      301 catttttact ggagctgact gctggctggt catctttcgg tcaccgacga tccaaaaacg
      361 tgtctggttc aactcattat cttggatatt ctcagctaag atctccaatc caacatgctg
      421 tgctgtttct aatgaagcaa tcgctgctgc gtcttctttc ggatgttctg ctacatacat
      481 tgctgcagca gttgttgatt ctgttgctac taaaggaaca ttaggataat gcgtttctaa
      541 aaactgttgt gattgtgcta gtgcttgtgg atgtgaaagg atttttgtaa tctttgttgc
      601 tggattgcct aaaagctgct gcttgatagg aagaacgatt tcacttttaa cttcgacttc
      661 tggatgtttg gaaagaagat cgattgtgtg atgaacagct ccttccaatg aattctctac
      721 aggaacgaca gccagatcga cttgtttgcg aaacaaagcg cgcaaacaaa cagggatgga
      781 agcatacgca gtcaattgtt ctgtaggaaa tagctgacaa gccgcctgat aagtgaatga
      841 actttctggc cctagatagc tgacgttcat atgcttttga tcctttctat aatggtatcg
      901 atgatctttt ctggtgtttg attggtcgtg ttgattcgat gtgtcgcact ttcttcatac
      961 caagaaagtc gttgtgccaa taatgctttt atttcttctc ttgtttttcc aagagctaaa
     1021 ggtcgaatag acgtttcatc ttgttggata cgatcaacta gacagtccgc ttctgcctct
     1081 aagtaaagaa caatcggctg tgtttttaaa aaacgccgat tttctgcact ttggacaatc
     1141 cctccaccag tcgcgataat tttgtcttca tttgacagct ttcttaaaat atctgactcc
     1201 acttttcgaa agcttttttc accgtaatag tgaaaatatt cggcaatcga caattggata
     1261 tgttcctcaa tctttgtatc taaatctaca tactcttttt gtaaacattg agctaaccct
     1321 ttaccgattg ttgtttttcc agcgcccatg aaaccaatca gcaaaatgct agccatgagc
     1381 ttctcctcct gacacaagtt ctgctaaatc gtcaaaaaat gccggataag agattgaaac
     1441 agcttcagct ccttctagtt cgcaaggtcc atcagctatc aaagcagcta cttgcagcat
     1501 cataccgatt cgatgatcgc ctctgctgga cacttttgcg ccatgcagag gtgtcggccc
     1561 atggatgatc agaccgtctg ctgtcgcttt tatctttgct cccattttct gtaattcttc
     1621 tgccaccgca tcaattctgt tggtttcttt tacttttaat tcttctgcgt ctttgatgat
     1681 cgtgattcct tctgcttgag tagccactaa agctagaatg ggcaattcat caatcagacg
     1741 cggaatgatt tctccaccaa tcgttgcttt cttcaaatga ctcgtcttga cacttaaatc
     1801 agccgattgg ttctgttcat tgtgattcgt ctgggtaatc gctgcgccca tctcctccaa
     1861 cacatctaag ataccagttc ttgttggatt gaccccgaca ttttttaaca acagttggct
     1921 ttccggaaca agaagtcctg ctgctagaaa gaaagcagct gatgaaatat cacctggaac
     1981 agttatctgt tgaccagtta atttttgcgg tccggtcact atgatcgttt tatcttccac
     2041 tgtgatcctt ccgccaaatt gacgaatcat ttcttccgta tggttacggg aacgttcttt
     2101 ttcaacgatt acggatgtgc cttctgcttg taatgccgca aacagcaaag cagatttgac
     2161 ttgcgcactg gctacaggca tattgtatcg gataggtgat aaagagcggg ttcctgtcac
     2221 agtgattggt agatactggt cattttcttc tccttgcaag tcagcgccga tctcgcgtaa
     2281 aggttccatc actcgcccca ttggacgttt agacaacgaa gcatccccga acagagtagt
     2341 cgtaaaaggc tgtccagcca aaatacccat caataaacgt atggtcgtac cggagtttcc
     2401 catgtcaatt gattcggttg gttggacaaa tgagtggcta ccgcgaccat gaatcttgat
     2461 cacctcttcc tcttcttcga tctcgacacc taattgttgg aaggcatgta aagtactcag
     2521 acaatcttct gcgcgcaaaa aattttgtat agtcgtcgtt ccttcagcaa ttgctccaaa
     2581 catgatgctg cgatgggaaa ttgatttatc tgctggtatc ctaacagtcc ctcttaatcc
     2641 atgtatttgt tgtaataatt gcatttatcc agcctcctat ttgatccagc aatgataact
     2701 ggtattagac ataatgattc tttttgcttg taacaaatcc tgttggtttt taaaactgat
     2761 ttgcagtatt cctaaaatat cttccctggt ctcttggatc ttcaaattaa tcaaagaaac
     2821 tcccgctttt ccgatcagtc cagttacttc tgcaataacg ccaggttcat ctggcacatc
     2881 gataaataaa tcaaaaaagg caggaatcgc gccttgctta tggacaggca actggtctcg
     2941 actttctttt gcccgattga aaaaagaaaa gatttccgat tggttctcag tcatgatcca
     3001 ttctgctatt tgtgtcattt cttgttgcca atctgccaat cgttccaata atatctgctt
     3061 attgctcaac aaaatatctg tccacatgac aggatctgag gaagcgatcc tagtgatatc
     3121 tcgaaagcct cctgctgcca gttgtttggc tcgcggatac tcttgattga attgttctgc
     3181 ctgattgact aagccagctg ccagaatatg tggaaaatga cttagcattc ctgttatacg
     3241 gtcatgttct cttgctgtaa gagttacata ttttgctttg gttccgcaaa atagttcttg
     3301 cagtttttca acatcttttt tcgtcttttc caaaggtgta aagatgtaat aggcattttc
     3361 aaatagttta gcatctgctg cctgtatacc tgatttatga gaaccagcca ttggatgtcc
     3421 accgacaaac cgaagatttt tctgctcagc ggatgtcacg acttgctgct tcgtactgct
     3481 gacatccgta atgatgacgt gatcagacaa gttgattctt tcaaggacat caagataagc
     3541 taaggttgta cggacgggaa cggctaataa gataacgtct gcttgttctg cacctgtttc
     3601 aaaatcactt gcaacgtaat caacgatctg ctgttctact gcttgctcac gggtttttga
     3661 ctgactatcc caaccaacta ctatcagttc tggattggtc tgacgaatac aaagtgccat
     3721 agaagcacca atcagtccaa gccccacgat aaaaacatgt tgtgccatct tatttgcccc
     3781 ctttttagta atgtttcaag tagtcacggt actccttcac tgcagctttc aactcaacaa
     3841 gcgaatcact tgggaatttt gacaatagct ctgtcgctac cacagtggct acaacatttt
     3901 ccgcaacgac gctagctgca ggaactgctg tactatccga acgttctaca cttgctttgt
     3961 aaggttcttt tgtatcgata tcaacacttt gaagtggctt gtacaatgta ggaatgggtt
     4021 tcatgacccc gcgaatgaca agtggttcgc cattggtcat tccgccttcg aagccgccta
     4081 gattattggt acgtcggaaa tagcctcttt cttcttccca taggatctca tccatcactt
     4141 ctgagcctgg taatgaacca gaagtgaatc cgaccccaaa ttctacgcct ttaaaggcat
     4201 tgatactaac cacagcttgt gcgattttgg catctagctt cgtgtcccat tgtacgtagc
     4261 ttcctaatcc agctggaacg cctccaacga gcacttctac aattcctccg atcgtatctc
     4321 catttttctt tgtctgatcg atcaaatctt ttattctttg ctcaactgct ggatcaacaa
     4381 ctcgtacttc tgacgcttca gaaatttctt tgatttcttg aatcgagata tttccaggta
     4441 tttctgcttg gattccacca agacaaatca cgtgtcctgc tacgatgatg tcaagttctt
     4501 ccagaatttt cttggcaatt gcgccgattg ctacacgcat ggtcgtctct cttgcagagg
     4561 accgttcaag cacattccga agatctcgat gatggtattt cataccgcct accaaatccg
     4621 cgtgtcctgg acgcggacgt gcaactcgcc gcagtgattg gtctttttca ggcacttctg
     4681 ctacagacat gacagttgtc caatttttcc aatctttatt ttctaccact aaagtgacag
     4741 gtgaaccaat tgttttgccg tgacggatgc cagacgtgat ccgaacacga tctttttcga
     4801 tcttcatcct tcctcctctg ccgtaaccag cttgtctgcg agcgagttcg tgattgattt
     4861 cttcttctga cagaggtaat cctgctggta gtccttcaat aatggctgtt aattctggtc
     4921 catgggattc tcccgcagtc atataacgca tcttacagtc ctcctaagta atctttgaat
     4981 gtctctgtgg gaacaggaac gatttttgct tgcccaatct tttctagcaa gatgatattc
     5041 aattggcttc ctcttgcttt cttatcatgg gttatcgcgt ggaataattc atctggatga
     5101 cttagatcgt acgtaatcgg cagatggaat ttttgaagta tcctttctaa agcagctgtc
     5161 gatccagttg gagacaagcc gattttttct gcatgagaag tgatggctac cattccaata
     5221 gctactcctt ctccatgact gactacccca tatcctgctg tattttcaat tgcatggcca
     5281 attgtatgac caaaatttaa aataagtcga tttccttggt caaagacatc ttcttccact
     5341 acttttcgtt tgatttccaa cgtagcagta atgattggaa cagcatgttc cagaagatct
     5401 ttttcgtctt tcagctcgtt cagctgtttc caaagcgttg catctgctat agctgctgat
     5461 ttgatgattt cagcgattcc ttctcgtaaa cgacgtaccg gcaaagtttt tagaacctca
     5521 ggatcaatca atacaccagc tggctggcta aatgttccga caaggttttt cgctttgttt
     5581 gtattgactg ctgtcttccc gccaatacta gaatctactt gtgcaagtag cgtagtcgga
     5641 atttgaacga aatggatccc acgcatgaac gtcgaggcca cgaatgctgc tagatcaccc
     5701 accattcctc caccaagtgc aagtatccca tcgctgcggg taaaatcatt atctgctaaa
     5761 aaatcgtata actcagctgc ttgttctaaa gatttactgg tctcacccgc cggtacgatt
     5821 gaaaggactg gttcaaaatt agcttgtctc aattggaaca tgattttttc tgcatatagt
     5881 ggagcaacgt ttgtatcagt gatcacagct actttttgag gtctccaaat cgtttgaagc
     5941 catgatccag cctgttctag tgcgcctttt tcaatatgaa tatcgtattt ttggttatca
     6001 acatgtacag taagcattcg tttcacttct ttcttttaaa aagctggaat taatcgagtg
     6061 tctgaatctt ttccattgct tctttcatga tcgcaacttc tttcatcatg cgtgagtaag
     6121 tcttttcatt caacgattgg ggaccgtctg accaagcatt tgcaggatca ggatggatct
     6181 ctacgatcat gccatctgct ccgcttgcca cacccgcacg agccattggt ggtacaagtt
     6241 cccaaattcc tgtaccatga cttggatcaa caatcacagg gaaatgagtt aattttttca
     6301 tcaatgggac agcacttaaa tcaaacgtat tacgtgtcgc tgtttcgtaa gtccgaatcc
     6361 cacgttcgat aaaaatgaca ggagatttgt cttcgactgc aatgtattct gcagcattaa
     6421 gccattcatt gatcgtaccg gaaattccac gtttcaatcc aacaggttta cctgttttac
     6481 cgacagcaga tagcagttta aaattttgca tattacgtgc gccaatctgt aggatatcgc
     6541 tgtactcagc caccatatcg atatgccctt catccatcac ttcagtaatc actttcattc
     6601 caaattcatc agccgcttga cggatgtatt tcaatccttc ttcttctaaa ccttggaaag
     6661 catatggaga agtacgtggt ttgaaggcac cacctcgcag aatttttgcg cccccggctt
     6721 tggccattcg cgcacattct ctgatttggt ctaatccttc gatagaacaa ggaccagcca
     6781 tcgtcacgaa actgccgtca ccaattttta ctccgtctac atccacgact gtattgccag
     6841 gatggaattc tcgagaagtc aatttgtatg tgtttaaaat acgcacagca ttttctactc
     6901 cttcatagcg tgtaaatgct ccttcttgta aacttcgcgt atcacctttt agtccaatca
     6961 ctactcgttc tgttccttga tcgattgaga ctttgaatcc ttctttttcg atatgatcga
     7021 cgactttttt tatttgttct tccgttgctt cttttttcat aattactatc attgtttttc
     7081 ccgctttctt ttgtttactt atctatcttg gctgattttc tctacttttt caaggtggtt
     7141 ttacattaat tcctactaaa tgaccgtgtc ctgttgttct cattttcgct ccttctttcc
     7201 tacttatgac ccagataatc aacaaaaaaa gatgtccctg taggaaattg cctctacagt
     7261 gaacatcttc aacgagcctt aaaaagcgct gcaaaaatat aaaatagcca ctatagatga
     7321 atttctctac gtggctgtaa gtcgtgttta aaaatcttta gccatcatag atacaaacct
     7381 ggtttgtttg tacccacgaa gtcatgaata caaacctatc taaaatagct aaagtaatac
     7441 gtattcagtt ttgtaagttt tctagatttc ataaaaaatc gctccaaact gatgtaagaa
     7501 ttgtacctag tatagacata aaaaaatcga ctgtcaatca tttttatgaa acttttttta
     7561 attttctgat agttaacttt gtttttcaga aaaataacaa ttggttttat tgaagaaaat
     7621 agtcatgaac aaaagtatct gagaactttt ttactctctt gttcatgaca tatagaataa
     7681 agcagctttg tcttgtaaac tccgtttttc aaataataat ctaaagattg ctactgaata
     7741 ataaagccga tttcatggtc ctcttcgctg ctgattcggt aaggtgactc aacatccgct
     7801 ccccagctat tgataccccc gactcctctt acttttgcaa aaatcgtaag aaccgttcgt
     7861 ctttctggcg gtagttcttc tatatgtgtt gcctgttcca gttcttctgc tttgaatggt
     7921 aaagcggaaa aagcaaatgg cgtatcctgc tgttcaaatc gcaacgaaaa atcttcttga
     7981 tctttatccg tattatccaa cgtttgattt cttgttagtt ctacccattt cgtttccata
     8041 tgcatgccgc attcttgggg aacaaggtat ggggaaacgg ggagtcctgc cactttataa
     8101 ataccttcga caccaccagc catccgatcc ggatatgttt cccctgatag gccttcatac
     8161 tcaaaaaatt ttgctttcgt tggcataatg aaccttaaac ccaatgcggg taattccggt
     8221 aatccttttt gtccataata atgcatattg acaagcattt taccctctcc agcgatttgg
     8281 taggaaattg tcacttgggt ttttggtact gtgttgattt ccagtgtaaa aatgatttca
     8341 accgtttcag cgtattcatt acttgaatat tgattattta ctggagcaat cggcaaatcg
     8401 atctttttat tgtccaccag tacttctatt tctttgactt ttggatacaa tccggctcct
     8461 aaccacatac cggatttaaa tccaaatccg ctacctctgt cattatcggt cgttgctcgc
     8521 caaaaaactg gaagtgtttc ccggtatagc cattcttttc cgtttttatt gagcgattcc
     8581 agtccgcctc ttgtataaga aaaaatataa tgcgtattcc ccgatttgac acctaatgta
     8641 cagtcgccga agacgattgc aactttttcc gtatccatat gacttttatt tactgtattt
     8701 ttcataataa tatcttacct cctgcatggc gggtttttct tgtccatctg caaaaacgat
     8761 cccattccct gagaattcat agtcacttgg tttatcgtca aagtcaccgc catagcgcat
     8821 cacttctctg cctgtgattg gatcagtcac ttggatcgcc tgatctttaa aatcccatat
     8881 atagcctcct tggtacattt cataacgatc aagcaacgtc atgtaagaat ccattccacc
     8941 taacgaattc cccatgtcat gcatatattc acataagatg aacggttttt ccgctttatt
     9001 ttcaaggtag tcaatgatct cttctggtgg cgcatacatg cagcttttta catctgaaat
     9061 cgaatcttcg aatgctggat ttggaaaatt cccttcataa tggacaagtc gatcggggtc
     9121 tacttttttg aagtaatcgt tcatttgtcg aatgttttct tccgcataag attcatttcc
     9181 aagagaccaa aaaagaatac ttggatggtt ttttaatgtt tcgaaattcg tttttgctcg
     9241 atcaagaaca gccgcctgcc actgaggtac tgcacctggt acgttgtaag aaggctcaaa
     9301 tgcgcccatt ttttgccatg agccatggga ttcgaggttc gtttccgcca tcacatggat
     9361 tcctgcttgg tcgcataaat aataccaagt gatctggttg gggtaatgag atgtccgtac
     9421 gccgttgatc tttgcatttt ttatgatagc caaatctttt tccatatctt ctatagtgat
     9481 ggcgcgtcct tttttcggat gccactcatg tcgatttacc ccgttgataa tcatccgttt
     9541 tccattgaag tagatgattt tatctttgat ttcgatttta cgaaaaccga aggaatagtc
     9601 aatatactcc gttactcttt tttcttcgtc tatcgtctcc aattcaagct tatataaatt
     9661 gggctgaaaa tgcccccaca gtgccacatt ttcaaatcca tcgatgcgaa tcgtcatttg
     9721 ctcttttaac ggatattgct gttgatagat ttccttccca tcaggatcta gtatcgctac
     9781 attcagctga ctgtttttgg ctacttgctc aaaagacaaa tctacttcaa gtttgccatt
     9841 ttgacatttg tcatctaagc gaggtttgat ccaaagatct tctaaatggt tttgtcctcg
     9901 agcaactaag aagacatctc tgaagatccc aaaaaatcgg aaaaagtcct gatcttccaa
     9961 gaatgcagct gtacttcttt tgtaaacctc cacagctaga atattttctt cttccaaata
    10021 aggtgttaaa tcaaattcag acggagtgaa cgaatcttcg gcatacccga taaaatgacc
    10081 attcaaccaa ataaacattg cttgttctac accagcaaaa tgaatcgaga tttgttttcc
    10141 ctttagacct tcttctaatc gaaaagtcgt caaatacgaa ccaaccggat tgtctttagc
    10201 agctgaaaag atcccttccg cctctttctc agggcttgtc gtgaaagggg gacggcgaaa
    10261 tacatgtccc tcccacggat acatcgtatt gatgtaatgg atcttgtcat aatttgctaa
    10321 ttcaatatga ctaggaacag tgatttcatc aaaagaacag gcatcaaaat cttttttata
    10381 aaatgttttt ggtcgtaccg aaggatttgg cgaataacaa aaacgccatg atccatttaa
    10441 agactgttta agggaggatg tggtttctac ttcttccctt gttgcataac aaacatgatc
    10501 gctatgtgca tttgattggt tcactctaaa aacttgtgga tcatctaacc attttatatt
    10561 tgctttcata cttttttcct tctctctcat aactttttga aatgaagcag ttctgtttgg
    10621 aaatcacctt gaaaatcggg gaagtaaagt ccaactgtca tcaattcggc ccccgaaaat
    10681 atccgcttcg aggtctgttc ctgataaagt gcctcatcat ctaaccccat cagatatact
    10741 tcatggaacg ctggttgagc tgaagctagt atcctcccta aaaacacaat agcttcatct
    10801 tgtttaggag acacaaacat ccatgcggca atattttctt caaaaggact cgccaaacga
    10861 taataatggc catactggat caccggacga atcgtttgat aggatataat ttggtctttt
    10921 acttgctttt cttcttcagg agacagcttt gtcaaatcca gttcataacc aaaaacagcg
    10981 ctcatagctg tttctcctct tgttttcaac gaagtagatc tgtcggtctg atgatttggt
    11041 acagctgata catgagctgt gatggacgaa atcggataag caaggcttgt tccatattgg
    11101 attttcatac gctccactgc atccgtgtta tcacttgtcc atgactgcgg catataataa
    11161 ataaacccgg catcaaaccg tccacctcca ccagaacagc cttcccataa gatttccgga
    11221 taatcagttg tcaaatgttc aagtagttca taaagcccga gcatataacg atgcgctact
    11281 tctccttgtc tctgtgggtc aagggtaatc gaatagacat ctgaaagcga acggttcata
    11341 tcccatttga tgtagtcaag aggaatcgta tctaaaatct ttctcatttg tttctcaatc
    11401 gtctgccgaa catctaacct tgtaaaatcc agtacatgtt gtgatctgga agcagaaggc
    11461 attctacctg gctcctgcat aagaaaatca ggatgtgttc gatacagttc agaatcaacc
    11521 gagatcattt caggttcaaa ccataaacca aatttcaatc ctttgctatg agcataatct
    11581 gctatttcac gtaatccatt tgtcaacttg ccttcatact cgaaccagtc tcctaatgaa
    11641 gaattgtccg catctctttt tccaaaccag ccatcatcta atacgaacat ttcgatgcct
    11701 agctcctttg cttcatcaat gatcgccttg attttttcac tattgaagtc aaagtaagtg
    11761 gcttcccagt tattgaccag aatcggccgt tcagcaaatt gatgacgacc tcttgcgatt
    11821 ctttcacgta gcaactcatg gtggatctga ctcattttgt tcaatccctg acttgagtaa
    11881 gaaaggatca tttcaggtgt ttgaaaagac tggcctgcat tcaattccca cgaaaaacga
    11941 tggctgttga tcccaccgac gactctcaat tgattgatct gatctttttc aagttcgaaa
    12001 gaatggttgc cggaataaac aaattgaagt ccgatcgctt ctccagtatt ttctgtcgta
    12061 tgatgatgaa ctaaagcaat aaagttattc atatggtgac tgctcgttcc tcgacggctt
    12121 tcaaatactt ttgttccaga taaaacagat tgccgtgaaa tctgtctttc attgacatgt
    12181 gctcctggca aagcaatgac ttcatcaaat cgcctagtat gggcaaagtc tagctggaaa
    12241 ctagccgctt tttctagttt gattgtttct ccggtttcat ttcttatctt tacagatcgt
    12301 gtgatcactg ctctatcagt aaatatggta tagaacaaat cgaaataaat cgctgtttct
    12361 ctatccttaa gtgaaatcac caacgtttgt gcctctgact tgtccatcac ataagtttga
    12421 ggcaagcctt cgatctctgg cttcccatct gtaatataat ggctgtcata tctcagatcc
    12481 agtaagtctg atccattctt gcgctgaatg atcgatgcgg gcgttcgaaa gtccatgctg
    12541 ccatgagaag agtattcttg cggtagtgta tctttcgaca gagaccggtc aggatttaag
    12601 ggaacatttc ctgaaaatcc acgatctctt cggggataat ttttattgtc tttgtactgc
    12661 ttgactggtt tgccaaaata aatatgcgcc aatacattgc tttcttctat ctttatcaga
    12721 taagaaagaa atgtatttcg taaatggaag actcggctct tttcatcaaa taagatgaaa
    12781 tttttatctg tttttctatg cgtaatatcc atcatttcac tcccaacaaa acggaaaccg
    12841 tttacttttt tttataattc ctgtatactg tttactaaac aattttgtca ataaaaattt
    12901 tttatttttt tgaggagtgt gtaaaaatgg ttggaattag agatgtcgca cgaaaggcaa
    12961 atgtctctcc tgctacagtt tcccgtgtgt tgaataacga tcagacgatc catgtggctc
    13021 agactactcg gcaacggata gtagaggcag ctgctgcgtt ggattatgac atcagtactc
    13081 gtaaatatat caaaaagaga atgccttcga ttggtgtgat ctctacgatc ggcaaagcga
    13141 gcgaagaaca ggatatctat tacaaacatt tgcgagtagg gatggaagaa gaagctcgac
    13201 gtctgcattt agggatgaat cgaatctata atctatctga taatcctaaa caatgggaag
    13261 atctagatca gttaggtgcg gtgatcgttg ttgggactgt tacgaaagaa tcgattaccg
    13321 ccttgttaaa acaaaacatg caaatagtcg tcgtggataa ccctgacatc cagcaagagg
    13381 tcgatatggt gtatgcagat cttgaacgga tgactaaaac cgtactggaa ttgttcttag
    13441 aaaatggtca taaaagaata gcctatatcg ggggatataa agtcgatatg gatgaattcg
    13501 gtgggaagca gatttctata aatgaaaaga ggatgcgtgc ctatcagcat tttatgatcg
    13561 atcatcactt agaaaaatat atgtcttatc atctaggtgg ctggacagaa aaacaaggtg
    13621 aggtacttgc gactaatcta ttagaaaatg aatcccctct tcctactgcc attcttgtcg
    13681 ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa acaaaaaaat atccaaattg
    13741 gagaagagat tgttgtagca agctttgata atatcgagac aatcgcaaac ctctcccctg
    13801 gcttgaccag tgttcaaatc aatgcgaaat caattgggca agcagctgtt cgaatggcat
    13861 tggagcgaat tgacggtata cgggacgaac caatcatttt gacttatcct gcacgattga
    13921 ttgttaggga aagttttatc ccaagaacaa aaaggtattg aaagagaggt tgtgaaaagc
    13981 cgcttcgttt cttcatactc aaaacgaaac ggctttttca caacctctta cttatactta
    14041 ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt cttttagcgt gtaatcaaat
    14101 tggtattcat gggtaatctc tttttttgta tctgtataaa acaaggattg cacgggtacc
    14161 tttaattctg tcaaccgatt ttctaatgct tgaccttgct cctggaatga gtatgcattt
    14221 ccgtcagtta tatacgttgg cggaaattcc ttagaaacat ggtcagccac actagcttct
    14281 ttcaattgct cactcgtttt ccaatctttt gttccaatca acgaccacgc aacggttttg
    14341 acgaaaaact tcataaatcg atcatccgaa tgctgctgtg ctgttagctt caaatcaaca
    14401 ggtccgcaat aagaaatggt tcctttcaga ctatcttttg gaagtagatg tttgattttc
    14461 atttgtttag catacgcatc attcgtttgt atagctgcat attgcaatgc gatttgtgaa
    14521 cctgcactat ctccaccaaa aaataattgt tccatgtcta gcaatttgtc gtctttttct
    14581 tcagctaata aatctttgat cagttcattc acttgaagaa cttgattagg gtattctgaa
    14641 tcaggcgcta attcataatt catcgcaacc actgcgatgt tggcgtctga aactaatttt
    14701 gtcgcaaatt ctttgacacc cgatttatct ccgccgacga atcctccacc atgtacccaa
    14761 aatagaacag gaacaggccc cttgctgttt tttggatagt agatgtcata agtattctct
    14821 ttatacttgg actcatacgt caaatctgtt tttttcgcca cgtttttttc tgcttgatcg
    14881 aacgtttttt tatcagtgat ttgaacagta tttgaaaaca tatggttgat caagaatgct
    14941 ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa acgcgatccc aagaatgatt
    15001 ccgaagaacc atccgataaa tttaagaatt ttctttttca taaggctccc tcagtagtta
    15061 gtattaagca ttctgattat accatagtgt tatttcctat caagctctcc tagatgattt
    15121 aatgattttc cccaaacttc ttctgcaatg cttttgacaa aagaaatttt ttccattgtt
    15181 cttcaaacaa aacattttct tcttctgttg aagcaaatcc acattgagga cttaagcaca
    15241 aacgatccaa aggcagatat cgtgtcgctt cgtagatccg gtcaatcact tgctggtgct
    15301 cttccatttc gccatttttc gttacagtca aaatctaaga caactaattt atcttcagag
    15361 actttctcta aaagaacaaa actcccagca cgttcactat cgtattctaa ataataggca
    15421 cggacatttt cttgagtaaa ataatgggtc tgctacgtcg tcatcccccc tgatgccgcc
    15481 aaaatcgaat aataatttcc gcgtcaaaca tgtatattta gtgttgaatc atttgagaat
    15541 atgaaaataa aaatcttttt gatcaatcag ttaatcaaat tgaaataacg aaaaatctga
    15601 gacagaatga cgttcattct gacccagatt tgattttgca caactcaaca gtccttattg
    15661 gcaaatcgcc aaaataatgt agactaacta aaaagactaa gaaacaataa caaaagttaa
    15721 cacttcttag tctttcttta gcacgtctat ttattaaaga ttttagggcc ttttctcatg
    15781 tttttacgat cattcttagg tgtttccatt tgtttggatc ggcctccacc ttgtttcttt
    15841 ttgatcaatt ctttcatttt ttctttttgg tccattttta ttctccttta acgaatgaag
    15901 cgactaattc gatatgatgg gtttgcggga aaagatctac aggctgaact ttcttcacgg
    15961 cataacctgc ttctgcaaat aacttagcat ctcttgcaaa agttgccgga ttacatgaga
    16021 tatagacgat tcgttctggt gacatttcga ttgctgattc aataaaatga tgatctaatc
    16081 cttttcgtgg agggtcaact acgatcacag atggtttgat ccctttttct tgccattttt
    16141 tcataacttt ttctgctttt ccgacttcat aatgaacatt ttcgatttta ttatttaaag
    16201 cattgaactg tgcatcttca attgcttctg gaataacttc catcccatac accttatcca
    16261 ctctgtctgc taaagacaag ccaatcgtac ctattcctga ataagcgtcg atcacaatgt
    16321 cttttttatc taactcggca aactcgatgg ctgtttggta cagatactct gcttgttgag
    16381 tatttacttg ataaaatgac tttgctgaga ttcgataggt ttttcctaat agctgatctt
    16441 cgatatagtc tctgccatat aaaacttttt ctttgtctcc taaaatcaca tttgtttttt
    16501 cttcattgat attttggatg accgaaacca cttctggcaa ttcttcatga atagcttcag
    16561 caatttcctt ggatttaaag aatttttctt tacgagtaac taaaacgacc atcatttcat
    16621 gggaataatg accacgacgg ataatgatgt gacgaagctg tccttcattt ttttcttcat
    16681 tatatcctcg tacttggaac cgttgcaaga tatctctgac tactttgatt gcttgatcga
    16741 tgacaggatc ttggatataa aaatcttcga tagggactag atcatggcta ttctttttgt
    16801 aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg gatctgtgct ttattacggt
    16861 agccgaatgg ttctgccatt ccgaccgttg gaagtacagg aacttctggc atcttagcaa
    16921 tcttcgccat tacatgctct acttgttttt gtttgaagac aagctgttgt tcatacttca
    16981 aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt gttttctaca ggctgacgat
    17041 caggtgattg tgtaataaat gtttcgactt ttccatatcc aaacttattg cctgctttta
    17101 ctactagtat ttctacttct tctccaggga gtgcattttc gataaataat ggataaccat
    17161 cgatttttgc tacccccatt ccttcataac tcaagtcgac gatttcgact gtatatcgtt
    17221 cgtttttctt tactatttgc tgttctgtca tattttgctc ctttaagcct atttttcaat
    17281 cctactgaac tctactataa atcagctaat tacacaacta aaaccggcac tgaccttact
    17341 acgttcagca ccggcttttg attaattctt ttgattaggt gtttcgtctg ctatttttcc
    17401 gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct tttacgaatt ctttgctgac
    17461 aacctctaat tcttcttcat ctgtacctgt gattgcttca tcaggaatct gatcggtatt
    17521 ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc atcggtgctt ccccgccgta
    17581 ttcaccatct aagttgatca ttactggtcg attgatatca tcttctgttt caacgaacaa
    17641 actgcttgtt ttggtataga ttattcttgg atcatcggca tgtcgaccgc cattcagcat
    17701 gagtgctgca atatgcaaga tctcaaagat atttgctgtc ttcacaatga ttaaagaaaa
    17761 cttcccatca tccaatttgg catctggcgc gatctgttcg aatcctccaa cagagttcgt
    17821 cagccctagg aaaaacatcg aagcatttcc gatatattcc ccttcatcat acttcaagcg
    17881 cattttgatt ggctttactc gagggagcat ctctgctcct ttcgcaagat aagcaagata
    17941 accaaaaata ctctttagtt ctgatggaac ttcataagtc aattctgtca aatagccacc
    18001 tgcagcgata ttgatgaagt aactgttatc agaaaggccg atgtccattt ttacggtttg
    18061 gtgttttttt atcacttctg ctgctgcttt aatattatct ctaggaattt ttaatgctcg
    18121 agcatagtcg ttcgttgtac cagctgggat gatggccatt ttaggacggt gttcaagcgg
    18181 agcgattcca ttaaccactt cgttgattgt gccatcgcca ccagctgcca ctactagatc
    18241 aaaacctagt tctgcgactc tacatgcttc attcttggct gaatctggtt ctggtgtagt
    18301 tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc tgtaggatat ccgccaaatt
    18361 ttttttcaat aattcttttc ccgaggtggg gttataaatt acgcgtgctt ttttcatttt
    18421 cagcctctat cgtttcgcta attcatcttt aagtagctta ttcacgacac caggatttgc
    18481 ttgtccttta gttgctttca tgatttgacc gactaggaag ccaaccgcac gatcttttcc
    18541 atttttgaaa tcatcgacag attgttggtt gttatccaaa atttcattaa tgattggcag
    18601 tagtttgttc ggatcagata attgcaccca accatttttt tcaactactt cttgggcttc
    18661 tccgccattt gtgatcaatt cacggaagac ttttttggcg atttttgagc tgatcgttcc
    18721 atcggcgatc aaacgaatca ttcctgctaa gttctcaggt gtcaattttg tctcagctaa
    18781 ttccagtttc tcactgttca agtaagcaga aacttcaccc atcaaccagt tagatgcttg
    18841 cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa tcagacattt cttttgtaag
    18901 tgtcaatacc attgcgtcat actctggaag tccaaactca cggatatagc gttcgcgacg
    18961 agaagctggc atctcaggca aactagcttt tactttttga atccattcgt catcgatcac
    19021 gaatttcggg atatcaggct ctgggaagta acgataatca ctagaccctt ctttgacacg
    19081 catcaaaact gttttattcg ttgtttcgtc aaaacggcgt gtttcttgtt ggatttctcc
    19141 tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa gctaaacctt ttttcacgaa
    19201 agataaagag ttcaagtttt tcaattctgc tttcgtaccg aactcttctt gtccataagg
    19261 gcgaagagaa atattggcat cacagcgcat agagccttct tccattttca catcgctgac
    19321 ttctgtaaat tggataattg atcgcaatgc ttctagataa gcgtatgctt cttctggtga
    19381 acgcatatca gcttcagaaa cgatttcgat caaaggcgtg ccttgtcggt tcaaatcaac
    19441 gtaagaataa ccgccaatac catgcatgtt ttttccagca tcttcttcta aatggacacg
    19501 ttcgatccgg atttttttct ttttaccttc tacttcgatt tcgatccatc cgtcatgacc
    19561 gattggttga tcaaattggg aaatttgata tgcttttgga ttatctgggt aaaagtaatt
    19621 tttccggtca aaatgagtat ctttagagat ttcacagttt aatgccaaag ctgctttcat
    19681 cccgaattcc agcgcagctt tattcattac tggcagcacg cctggataac cccagtcgat
    19741 cacgtttgta ttgctgtttg gttctgcacc aaaatgagct ggcgctggag agaaaatttt
    19801 tgagttggtt tttaactcga cgtggacttc tagtccaatg actgtttcaa aattcattcg
    19861 tctttccctc ctaaaatagc tggttgttgt ttatggaatt ctgttgcttg ttcaaacgca
    19921 tatgccgctt gatacatcgt tttttcatca aaataattac cgatgatctg caatccaact
    19981 ggtaatcctt ctgataaacc tgccggtaca gacatacctg gaagtccagc caagttgaca
    20041 gggatcgtta agatgtcact catgtacatt gtgattggat cgttaatgtt ctcaccgatt
    20101 ccgaatgcaa ctgttggtga agatggcccg atgatcaagt cataatctgc aaagactttt
    20161 tcaaaatctc gtttgatcaa tgtacggact tgtcctgctt ttttgaagta agcgtcataa
    20221 taacccgcgc ttaaggagaa tgtacctaac atgatacggc gttttacttc ttcaccgaat
    20281 ccttctgaac gtgagttgac atatacatca tctaatgatt tcacattttc agagcgataa
    20341 ccgtagcgga taccatcgaa tcgttgcaaa ttggaacttg cttctgatga ggcaatgata
    20401 taataaacag ccacaccgta ttttgaatga ggcaagctga cttcttccac aacagcacct
    20461 aatgaacgga atgtttctgc tgctttcaat acagcttctt ttacgccagg ggccacacct
    20521 tcgcctaagt attctttagg taaagcaatc ttcattcctt tgatgtcgcc tgtcagtccc
    20581 tctgcaaaat caggaactga aacgcctgaa gaagtaccgt ctttttcgtc ataaccgctg
    20641 atagctgtca atgctaatgc attatctttc accgtacggg tcattggtcc gatttggtca
    20701 agagatgaag caaaggcaat caaaccaaaa cgtgagacac ggccataagt aggtttcatc
    20761 cctacgatgc cgttaaaagc tgctggttgg cggatacttc ccccagtatc actacctaaa
    20821 gaaactggaa tttgtccagc tgcaactgct gctgcagaac caccagaaga gccaccaggt
    20881 acttttgttg ggtcccaagc attttttgtt ttcttgaaat aagaagtttc tgtacttcca
    20941 cccatagcaa attcatccat gtttagctta ccaacaggga tcatatctgc ttgatagatt
    21001 ttatccatga ccgttgcatc ataaattggt tcaaaattgt aaaggatctt agatgcagcg
    21061 gttgtcaaga tatcttttgt tacgatatta tccttgatac caattggaat accagcaagg
    21121 acgttgcttt catcaatccc tttttcatct aaagcttttg cctgttccaa tgctttttct
    21181 tcgttcaatg taatgaatgc ctcgacctca ggctctgttt ctttgattcg ttcaaatgtt
    21241 gtttttgtca aatcttgtac agtgatttct ttcgatacaa gcatatgatg cagttcttct
    21301 agagaacgat tgtgtaattc tgtcattatg cgccagcctc cccattatcg atgattgcgg
    21361 gtactttgat aaagccgtct tctgtttccg gcacattttt caataattca tcgcggttcc
    21421 atccagcttg cgcccgatct tgtcgcatca cgtttactgt ttcaaccaca ttcgacgtaa
    21481 atggaacacc ttccgtatct acttcttcca gcatttcaac catatcgatg attttaccta
    21541 attggtcggt aaaacttgca agttcttcat ctgcgaacgc aagttttgct aatttggcaa
    21601 cgtgtttcac ttgttcttca ctaatcgcca tactacccct tctttctaaa cgagttaaaa
    21661 actttgctct ctattttaac acaagagtgc cgtgtctgaa attgtttatt gaaatttgtc
    21721 tgaaaaaact catttttcat tcttctactg agcgactttt agccaatgca tctgccattt
    21781 gatcttctgt ccaaacttcg attcctaatt cttgtgcttt ggtcagcttg ctgccagcat
    21841 cttctcctgc taccacgata tccgtttttt tcgatacgct accagtcact ttcccgccaa
    21901 gattttcgat cgtctctttt gcttcttcac gagtaaatcg tgttagttta ccagtaagga
    21961 ctactgtttt ttctttaaaa ggagactcga cttcttgcaa ttgggcattt cgaagccctt
    22021 tgtattcgaa attgacgcct gcttgtttta attcattcat caattcatgg acttcttcgt
    22081 tactgaaata agtgactaca ctgtcggcaa tgatatcacc gatcgtatca agcgcaataa
    22141 tcgactcata gtcactttta ctcaatgttt ctagatcacc gaaatgttct gctaaaattt
    22201 ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa aatcaaccgc tcgaccgaat
    22261 tatctttgct gttgtcgatt gctgtcaaaa tattatttgc tgatttttct tttattttgt
    22321 ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc agcaacatct gctactagtt
    22381 ttttatcata catctgttct agtacgcgcg gccccaaccc atcgatattc atcgcatttc
    22441 ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg gcatttagga ttgatgcacc
    22501 gtaaggctac ttcttcatcc aaatggacaa gttcacttcc acaaactgga caatgtgtag
    22561 gcaattggta ttcttcgctg tctttaggtc gtttatctaa cacgacttgc gaaacttctg
    22621 ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt caagcggata tccttcaact
    22681 taatataatc accgttatgc aggctggcac ggcttactgt cgttcctgct actcggactg
    22741 gtgtcatgat agctgttggc gttactaccc ctgttcgacc gactgtccat tcgatgtttt
    22801 cgattagcgt ttcaacttct tctggcggaa acttgtaagc tgctgcccat ctcggtgctt
    22861 tgatagtaaa accaagttga tcttgtagcg agaattcatt cactttgatg acgattccat
    22921 cgatttcata gggcaaatcc acacgtttgt catgatattc ttcaatgtat gaccacactt
    22981 catcaatcga atgacataga cgtttttccc gattcgtgtg gaatccaatt ttttctagct
    23041 cttctagcgc atcatactgg gtttttgctt gcataggacc gaaatcagct actgtgtata
    23101 aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc taactgacgc aaagaaccag
    23161 ctgcggcgtt ccgaggattc gcaaagatat cttttccctc tgcttctctg tcttggttca
    23221 attggacaaa tgaccgtttt ggcatgaaac attcgcctct tacttcgatg ttcattggtt
    23281 cttttaattt gattgggacc gaccggacag tttttaggtt ttcagtgatg ttttctccta
    23341 cagttccatc cccgcgagtc gcacctctca cgaaatttcc atcttcataa cgcaaagaaa
    23401 cggaaagtcc atctattttt agttcacagc aatagtcaac tacacgtccc actgcttttt
    23461 gtacacgttg atcaaaagct atcagctctt ctttgctgaa aacgtcgttc aaactgtaaa
    23521 gcggaatatc atgagtgact ttttcaaaac cttctaatac ttttccgcct actcgttgag
    23581 tgggagaatc agacgtgatc aaatcaggat attgttcctc gattgctact aattctgcgt
    23641 attctttatc ataaacatag tcttccactg ttggtttatc ttctacgtag tattcgcgag
    23701 accattggtt cagtcttgta cgaagttctt ctgctcgttt agcagcttcg tcaatcgtca
    23761 tttcatgttc catcatttac cctccactac taagcttttt caattggcgc aaaggcagcc
    23821 aaaagacgtt ttactccttg acttgggaaa gccacatcta gttctagatc cttcgacgtc
    23881 ccgcttactc ggacgactgt tcctagaccc cattttttgt gtttgacttt atctccaggt
    23941 ttccagctgt tattttcccc tccagtagca cttttagagg gaacaaccgc tgtctctggc
    24001 tgtttatagc tatatgctgt ttttttagcg aatacaggcg tttttttagg cgtaggacgc
    24061 atgccttcga tttccagtaa ctcctgatcg atttcttcta caaaacgaga aggtcggtta
    24121 tattgcgtcc gtccatataa tgttctggag aaagcatttg ttaaatacaa cgcttcttct
    24181 gctcgcgtga tccccacata tgcgagacgg cgttcttcct ccagttcgct ttcttccatc
    24241 aatgctcgag ataaaggaaa aaccccctct tctagaccaa tcaggaatac aactggaaat
    24301 tctaatcctt tggctgcatg caaagtcatc aatgtcactt gggaagcatc ttcttctaaa
    24361 ttgtcaatgt cggaaacaag tgccagatca tttaagaaga ccgttagttt ttcttctggt
    24421 gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt cgaattcttg tgtgaccgtt
    24481 aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta atgtattttg gatttttaag
    24541 tcttctagat atccgctacg atccaaaacc tctttcgtca attctgtcac tgtcaagtat
    24601 gggatcatct gagtgacttc ttggatcatc tctccaaatg cacctaattg ctgtcctgct
    24661 tttccaccga tatttgccag atccacattt tgcgctgctt caagcagtgg ccattcatga
    24721 agagaagcaa aactgcgtag tttttcgata gatcctggtc cgatgcctcg tttgggcgaa
    24781 ttaacgatac gctcaaaact gattgaatct tgaggattta ccagcacatt caaatacgct
    24841 aaaatatctt tgatttcttt acgatcgtag aacttgtgcc ctccaaccat tttataagga
    24901 atatttgctt tcaacagagt ttcttccatt acacgagatt gtgcgttcgt ccggtacaag
    24961 atggcaaagt cgccgtaatt ccgatgattg cttcgaatct cttcttgcat ccggtctacg
    25021 ataaaacggg tttcgtctcg ttcattatct gcacggtagt aagtgatctt gtttccttct
    25081 ttgttttctg tccacaagtt ttttggtttt cgattgctat tgttttcaat gacttggttt
    25141 gctgcgctaa ggatgttttt cgtggagcga tagttttgtt ctagcaaaat tactgcagca
    25201 tctggataat ctttttcaaa atccaagatg ttctgcatat ctgcaccgcg ccagccgtaa
    25261 atactttgat ctgcatctcc cacgacgcac aggttcctaa atctacctgc caataaattg
    25321 actagcgtat actgtgcgtg atttgtatct tggtactcat ctacatgaat ataatggaat
    25381 ttgttttgat aatacgtgag tgaatcagga tgttcttcaa ataatcgaat cgtattcatg
    25441 atcaggtcgt caaagtccat acattgatta tattgaagct ctttttggta agcagcataa
    25501 cattttgccg caatctcttc gtaataactg ccttgcattt ttgcaaaatc ctgaggggtc
    25561 tgcaggctgt tttttgcttg agagattgtt cctaatatac ttcgaggatc atattttttc
    25621 ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg tcagctgttc agaagaatcg
    25681 atgattgtaa agttccggtt atacccgata aaatcaacat ctcttcttaa aatacgcaca
    25741 cacatcgagt gaaaagtcga tacccagaca tcttcgcctc cagatgcgag aatcgcattg
    25801 acacgttctt tcatttcttt tgcagcctta ttggtaaaag taatcgctag gatgttccaa
    25861 ggattgactt ctttttcctc aatcaaatag gcaatgcggt gtgtcagcac tctcgtcttt
    25921 ccacttcctg cacctgccat tagcagtaat ggtccgtcag tgtgaagaac agcttctttc
    25981 tgacgggggt tcatcccgtt taataattca gctttggatg acattaacaa catcctttct
    26041 tttatcaatc ttgttaatta tagcattttc ttggctttca ttaaactagc tagttcaaaa
    26101 tcatttgttt ctttttatgg atttttcctt gtcaattcgt tgatttcgcg ctaaattaaa
    26161 agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca agaaattaga taagactcag
    26221 atatatttta ttctaaaagg aatatttgtt ggactgctga caggaatcgt cgttagcctg
    26281 tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa gtatctatga aatgagtcgg
    26341 gaacagccga tttacttaat tggcctagct atttgctgca tccttgcagc atttttcgtg
    26401 ggttatctgg tcaaaaatga accggatatc aagggaagcg ggattccaca agtagaggga
    26461 cagcttcgcg gggaattatc aatgaactgg ttctctgttt tatggaaaaa gttcattggc
    26521 ggtgtacttt cagttggtgc gggccttttt ctaggtagag aaggaccttc tatacagctt
    26581 ggcgcatcca ttggacaagg agctggacaa ttgtttcgga gtccatcttc agaagaaaag
    26641 attttaattt ctagcggtgc aagtagcgga cttgcagcag cattcaatgc tcctatcgca
    26701 ggattactct ttgttttaga agaaatccat cactcctttt ctccacttgt ttggctgaca
    26761 tcatttgctt cagcaatcac tgctaacttc gtctctcttt actttttcgg cttgagacct
    26821 gttttatatc taggtgacct tccttctttg ccgttgaaat attatggttc cttagtgatc
    26881 ttaggagtac ttctaggcat cttaggtttt atttatcaga atgtcttgct ggctttgcca
    26941 aaattttaca accgattgcc tttaccagct tattttcacg ggttcgttcc tttcttgctg
    27001 attttgccaa ttggttattt atggcctagt actctaggcg gaggcaatca aatcgtttta
    27061 gcttttggac atgcttcctt gccaatcatt atgattatcg gtttgtttgt tcttcgattc
    27121 gttttttcca tgatttctta cggtgggggt cttccagggg ggattttcct tccgatattg
    27181 acattggggg ctttaatcgg tagcttttat ggaaacatta tggttgattt cgggatggat
    27241 ccaatctaca tcaaggattt tgtcgttatt gccatggctg gttatttcac agcgattgga
    27301 aaagcaccgc tgacagcaat tattttagtg acagaaatgg ttggctctct taatcatctg
    27361 atgcctttag gattggctgc tcttgttgct tatatcgtca atgaccttct cggcggtaat
    27421 ccaatttacg aatcattatt ggaacgtcta cttggaaata ctcagaccgc ctcactaaaa
    27481 ggtgcaagga caacttttga ttttccagta acggcagaga gttctttaga cggattaatg
    27541 gtacgagact tcaattggcc taaaaatatg ctgctaatct ctatacgcag aggaaatcag
    27601 gaaatcttga ctcacggtga tacgatcatg aatgtaggag atattttggt tatcttgact
    27661 gatgaagcaa acttgccaag aatcaagcag gaaatcgagg ctaaatcttc tatcacaaat
    27721 ttgaagcaat cattttaacg aacatggaaa aactatttta caacaactat tcatctacac
    27781 tacttgattc tatatgattc tataaaaaag aagaggctgg gaaaacccag cctcttcttt
    27841 tttatttcta tgcttctgct actgcaggag cttttctcaa taaaccataa atcacaccag
    27901 atacgactgc tccgatagca ataaacacca aatacatgac cgggtgactc agtaagaata
    27961 caacgaaaat cccgccgtga ggagccagta atttgattcc gaaagctccg actaatccgc
    28021 cagttaaagc agaaccaata ataaaacttg gaatggcacg aatcgggtca gctgctgcaa
    28081 atggaatcga tccttccgtg acaaacgata gtcccatcac gatgttcgtc aaaccagcat
    28141 cttgatctgt tttgctgaat ttattcttaa acaaacgtgt agcaacgaaa attgccaatg
    28201 gaggtaccat tcctccagcc ataacagaag ccatcacgac acttccgcca gttgcaacag
    28261 tcgtagctaa agttgctgta ccaaacacat aagcggcttt gttgattgga ccgcctaaat
    28321 ctgccgccat cattccagct aataatgcac caagtaaagc agcattcgaa ccactcatac
    28381 ttgtcaagaa accgttcaat gcatcattga ttgctttcat agggacattg accagcaaca
    28441 tcaagaaacc agtaatcagt aagccaaaca ctggataaaa gaggatcgtt ttaattccat
    28501 ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa tacgatgaca taaccagcta
    28561 agaatccacc aatcagtgca cctaagaatc ctgcaccgcc tgtattagct aatgcaccag
    28621 cagcaaatcc aacgatcaaa ccagggcggt caccgatact tgaagcaata aatcctgcta
    28681 ataccggcag catgaaacca aatgcagctt gtccgatttg gttgaaccag ctagcagctt
    28741 gattgtaatt ccctagattt gataattgat catgtggcac gccaataaat tggtcgatca
    28801 tgaaagaaag cgcaatagcg attcctccac caataacgaa aggtaacatg tgtgatacgc
    28861 cgttcatcaa atctttgtaa attctttgtc cgatagtacc atccgcattt ccttcttctg
    28921 aactgctttc ttttccatca ccatggaata ctggtgcagt accactaagg gctagattga
    28981 tcaactcttc tggtttacgg ataccatcac tgacaggacg attgatcagt tcttttccgt
    29041 caaagcgatt catctctact tttttgtctg ctgcaacgat tacaccttct gcacgggcga
    29101 tgtcttcagc agtcaatcgg tttttgattc cttctgaacc gttcgtttcg actttgatct
    29161 ccacacccat ttctttcgct tttttcttca aagcatcttc tgccatgtaa gtgtgagcga
    29221 tccctgttgg acaagctgta acggcaacaa cgaattttct ttctggatga gcagaagctt
    29281 ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc tttttgctct tcagctgttt
    29341 ggaacaagtc ttggacttct tctggtgtat ttgcttcttt taattttcct acgaaatctg
    29401 catcaatcag caatcgagat agtgctgcta atgcttgcaa atgtgtgtcg tttgcgcctt
    29461 ctggagctgc gatcatgaaa aacagataag ttggctgtcc gtctaaggct tcataatcta
    29521 ctcctttttg gctttttgcg aataaaactg ttgcttcttt aactgcactg tttttcgcgt
    29581 gaggcatggc gattccatca ccaagacctg tagaagtttg tgcttcgcgg gctagaattc
    29641 cttctttata tgtttcgata tcggaaatcc ggccggcatc aaacattttt tggaccattt
    29701 catcgattgc gcctttttta tcagtcgctt gcaaatccat gatcatcgct tctttgacca
    29761 ttaaatcctt gatattcatg attatcctcc ttgaaactaa tcaatttttg tgatttcaac
    29821 ttctggcagc aactcatcaa tcaattcgcg agctgccaag tcatcagaaa aagctgttgc
    29881 gcttccgcaa gcaactcccc atttaaaggc ttcgattgga tctttcgtac ggctgtaagc
    29941 cccaataaag ccagcaatca ttgaatcccc tgcacctact gaatttttca atgggcgaac
    30001 taatacattc gttttataag ttccttgttc cgtgaagagc aaagcaccgt ctccagccat
    30061 tgagatcagc acattctgtg ccccttcagc tagtaatttt ttcccatacg gaatgatttc
    30121 ttctacagat tggaaggttg tgttgtaaag ctcagctaat tcgtgattgt ttggtttgat
    30181 caacaacggt ttcttaacaa gtgcattttt taggtcatca cctgttgtat caatcacgaa
    30241 atctgcacct ttttctcgaa tgatatcaat cagttcttga taaaatcctt ttcgcaggct
    30301 agctggcgcg cttcctgaca atacgacgat atctccttgt cctactgttc ctaatgcttg
    30361 cttcaactca tcgatttctt catctgaaat agatgggcct aagccattga tctcagtttc
    30421 agtgtctgat tttagtttga tattgattct tgtatcttct tttacggttg tgaacaaaga
    30481 ctggatttgt tctttttcaa gccacttcga aataaattcg ccagtgaacc ctcctaaaaa
    30541 ccctagtgct gtagattgag aatcgatccg tttcaaaata cgggaaacat tgatcccttt
    30601 tcctccaggt aacttgaagt cttccttcat tcggttcaaa tcgcctagtt ttaacccatc
    30661 tacgcgtacg atataatcaa ttgatggatt aagtgttact gtgtaaatca ttgtattgcc
    30721 tccttaattg ttgtcttttc acggaattca ttaacgaatt ctaatggaca acgctcggtt
    30781 atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg acaactggtt gaatttggta
    30841 tgatcaatca atacaaatgt ctcttcggaa tttttcatgg ctaaacgttt aacagctgcc
    30901 tcttctggat ccggagtcgt gaatccttgt tcaagatggg caccgttcat tcccataaat
    30961 gctttattaa aatgaaaatt ttttagttgt tctatcgcat tagatcctaa aacagcatta
    31021 gttgccagct ttatcgttcc acctaaaata atggtagcaa gctccaaatc aactaaattt
    31081 gccgcatgtt gaacggaatt tgtcactact gtgatctgtt ttccttgcag aaaaggaatc
    31141 atttctaacg tagtagagcc agcgtctaaa taaatgacat cttcgtcatt aatggtttca
    31201 gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt tgagtgattt ttcactcata
    31261 ttctgctcaa aattcaataa ttgctctttc ttagctccgc catgaactct ttctagaaac
    31321 ccactattct ccagctcttg taaatcacgt cttatcgttg attcagaagc attcaaaagg
    31381 tttacaagat cttgtgattt cacgattttc tgctgttcaa gcaagtctag gattttttgt
    31441 ctgcgttcct ctgtaagcat tttcacgatc acctcaatac aaataatagc atattttttt
    31501 tctactagca atataaatca gtcaaaaacg ttcataaaag ttataaatca gtcaaaaaaa
    31561 aaaataaaaa accagtcaga aagactggtt ttttattttt ttgtctatca tcaagccgtt
    31621 ttatcgtttt ttattttcgc ttcttgtttt tttcatataa acagaagcag gttttctttc
    31681 cttatgaaga agataataat aaagtcccat cccgccaaac atgaccaaaa aaccgatgac
    31741 tgatcgaata ggaaaattag gaacaaaaaa gagacagacg gataataatg atgccggaac
    31801 cattattcga taatagagtt gtttatccat tttcttcacc tcatattttt tgtaaagtaa
    31861 tcaattgttc taattctcgg taatctaaaa attgcacacg cagatgctgc tcacctttct
    31921 tgacatcgaa ttggatactt cgccgctttt tctttattcg tttgaaggta acatcagaga
    31981 gtctatgttt gatttccttt aacggtaatt ctgacaggga ttctgaagga acatagattc
    32041 ttaaggataa aagatactct tgatattttt catctgttac ctctacttct agatattccc
    32101 taatataata acaataagct tcgattccat tatccaaagc aacagtcgga tcgataactt
    32161 tatagcacat catgcgtgat cgattctcgt caacccaagg aaaatacgcc tgagcattta
    32221 gtgttccgcg aacacgcctt tctttataat ctccttgttg gatatcataa ctttttgtta
    32281 caaaaaatga agcattcttt ttaggaacag gttgcatttg tgcattctga tactggacag
    32341 attggatcgt atggtcagtg taaatgtatt caacgaatgg cggtttcaat aaaccgacac
    32401 cattgtccca agcaagatga tcaaacccaa gctggtccct aaaataggat tgaaacattt
    32461 gattgtttgg cttttgccag ttaaaggggt ctcgataaag ctggatacat atgacttcgt
    32521 tttctaattc cttcactttt tctacttgtt ggatgtcgag aaaaatcggt ttagggatga
    32581 tttgatgata atagcgacta taatacattt cccaacacga agtgaaacat aaaccttggt
    32641 aaaaaagatc gtatcctgga aattggttgc aatcgactac tacttccccg ttttgttgtt
    32701 tcattttagg taaatgttcg atttcatcct ttttctcaaa caatagccgt tgagagatct
    32761 gttttgtatt gtgatagata aactcgctat aagctcgcat ataggcgaac accccatgag
    32821 caatcatttt tttctgaatg aagtctcgaa tcaaaaattg cttttctttg aaaacagaga
    32881 tagacaatac attttttatc aataattgat tatcttccaa gcgaaaacta cattcgttct
    32941 cgtcatttcc tacaactaac caactttcgt ctcttaccga tgctagcatt attttttctt
    33001 gaagctttcg aagatcatac ggatccgctt gttgtgtgaa agagcggata aatgttggct
    33061 ggatcaacag acgtgtcaaa atagcccaat cgcttatttc ctgttttttt tcttcttcat
    33121 ttaagtctgt gagctggata gtcatcaata atttatcttc
//
LOCUS       DAXLPI010000018        33126 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXLPI010000018 DAXLPI010000000
VERSION     DAXLPI010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.018"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(93..950)
                     /locus_tag="ACF3T5_000750"
     CDS             complement(93..950)
                     /locus_tag="ACF3T5_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFD3631382.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(1058..2361)
                     /locus_tag="ACF3T5_000751"
                     /pseudo
     CDS             complement(1058..2361)
                     /locus_tag="ACF3T5_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2358..2867)
                     /locus_tag="ACF3T5_000752"
     CDS             complement(2358..2867)
                     /locus_tag="ACF3T5_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631383.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(3042..5177)
                     /locus_tag="ACF3T5_000753"
     CDS             complement(3042..5177)
                     /locus_tag="ACF3T5_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFD3631384.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(5260..6915)
                     /locus_tag="ACF3T5_000754"
     CDS             complement(5260..6915)
                     /locus_tag="ACF3T5_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD3631385.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(6917..7231)
                     /locus_tag="ACF3T5_000755"
     CDS             complement(6917..7231)
                     /locus_tag="ACF3T5_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFD3631386.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            7401..7661
                     /locus_tag="ACF3T5_000756"
     CDS             7401..7661
                     /locus_tag="ACF3T5_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFD3631387.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            7675..7989
                     /locus_tag="ACF3T5_000757"
     CDS             7675..7989
                     /locus_tag="ACF3T5_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFD3631388.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(8719..9273)
                     /locus_tag="ACF3T5_000758"
     CDS             complement(8719..9273)
                     /locus_tag="ACF3T5_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFD3631389.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(9399..11327)
                     /locus_tag="ACF3T5_000759"
     CDS             complement(9399..11327)
                     /locus_tag="ACF3T5_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFD3631390.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(11633..12349)
                     /locus_tag="ACF3T5_000760"
     CDS             complement(11633..12349)
                     /locus_tag="ACF3T5_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFD3631391.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(12350..14173)
                     /gene="glpO"
                     /locus_tag="ACF3T5_000761"
     CDS             complement(12350..14173)
                     /gene="glpO"
                     /locus_tag="ACF3T5_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFD3631392.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLIPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(14187..15683)
                     /gene="glpK"
                     /locus_tag="ACF3T5_000762"
     CDS             complement(14187..15683)
                     /gene="glpK"
                     /locus_tag="ACF3T5_000762"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFD3631393.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(15819..17279)
                     /locus_tag="ACF3T5_000763"
     CDS             complement(15819..17279)
                     /locus_tag="ACF3T5_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3631394.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(17269..18564)
                     /locus_tag="ACF3T5_000764"
     CDS             complement(17269..18564)
                     /locus_tag="ACF3T5_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD3631395.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(18896..19573)
                     /locus_tag="ACF3T5_000765"
     CDS             complement(18896..19573)
                     /locus_tag="ACF3T5_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFD3631396.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(19603..20694)
                     /locus_tag="ACF3T5_000766"
     CDS             complement(19603..20694)
                     /locus_tag="ACF3T5_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFD3631397.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(20795..21229)
                     /locus_tag="ACF3T5_000767"
     CDS             complement(20795..21229)
                     /locus_tag="ACF3T5_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFD3631398.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(21813..23270)
                     /locus_tag="ACF3T5_000768"
     CDS             complement(21813..23270)
                     /locus_tag="ACF3T5_000768"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFD3631399.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(23267..25162)
                     /locus_tag="ACF3T5_000769"
     CDS             complement(23267..25162)
                     /locus_tag="ACF3T5_000769"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFD3631400.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(25220..26995)
                     /locus_tag="ACF3T5_000770"
     CDS             complement(25220..26995)
                     /locus_tag="ACF3T5_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFD3631401.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(27045..28541)
                     /locus_tag="ACF3T5_000771"
     CDS             complement(27045..28541)
                     /locus_tag="ACF3T5_000771"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFD3631402.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(28735..30357)
                     /locus_tag="ACF3T5_000772"
     CDS             complement(28735..30357)
                     /locus_tag="ACF3T5_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFD3631403.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(30807..32120)
                     /locus_tag="ACF3T5_000773"
     CDS             complement(30807..32120)
                     /locus_tag="ACF3T5_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFD3631404.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(32365..32589)
                     /locus_tag="ACF3T5_000774"
     CDS             complement(32365..32589)
                     /locus_tag="ACF3T5_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631405.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(32609..33031)
                     /locus_tag="ACF3T5_000775"
     CDS             complement(32609..33031)
                     /locus_tag="ACF3T5_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD3631406.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
BASE COUNT         9461 a         7107 c         5512 g        11046 t
ORIGIN      
        1 ataactaaag agagatggga catgtagtgg cactcaagaa ttagattttt cagtctaact
       61 tcgaggtact ctaccttccc atcctctctt ttttatttct ttttgcggat gattccgtaa
      121 gctgctgcga ctaacacacc cgcccctgct aaggaataac tgacagaagc tgttttttca
      181 tttgtttttg gcaaagtctg acttgttgat ttggatggct ctgtcgtttg ctgaactgtt
      241 tctgtcgtgc ttccagaggt cactgatttt tctcctgccg aatgcaaaat atcttttatt
      301 cgttggaaac tagctgctaa gtcagctttt tttgcttcaa aatcattagc aactgtttgt
      361 gcttcgctga tctttccgtg gattgcttga cttgccgctg tgatatccgc ttgagtttga
      421 cttagctttc cagaagctgt ttgagcaacc tgatttaatt gctgattgaa ttcttttgct
      481 tgttggatct tcccgtcaag atctgtttgg acagcttccg cttttgcttt cgcttcggca
      541 agctttttct caagttgtgc acggtcattt cccactttat ttttaagatc agctaaatct
      601 tgatcgattg ctgcttggtc ttctttcaat ttgttttttg cagcttctaa tgcttcttcg
      661 agttcttttc ttttattttc caagtcttgc aaatgatcga tttggtcaga agaggtagaa
      721 ctatttattg atgtatcttc tgaagatgaa atcgtttctc ctgaattatc tgtcggtaca
      781 ttggaagtca tttctgtttc cgaggcaccg ttttcagcac cagaagagct tatagttgtt
      841 gaaggttctg tttgagcagg ttcagaagtt tccgcaaaag ctgtctgtcc caataagaaa
      901 atactggaaa cagcaattgg ccagattaac ggcttcattc tttttttcat cacttttccc
      961 atccttacta ctttttaact aatttcttat tataagcaaa aatgtttgga agaaccataa
     1021 acacaacagg ctgtttgtct aaaaaacggt gcatctttta tttttttgta cgttccgggc
     1081 ttttccatgt cccgcttcca taacctaaaa tcgttttgac tgctggaatg aaagtcgtca
     1141 cgatcgttat cgcgttcact atccagaaca aaagcatata tagtggtgcg aaaagaaggt
     1201 atttcaattt tcttcgtcga tcatctacaa tcagcgaagc tagcaactgg aaaaatcctg
     1261 cgaccatttc aaagcagatg aacacaaaac tgaatgtaaa agtaatataa atctgttcgt
     1321 agtttccttg atatacataa tgcccgatta atccagcaaa aagaactacg gaaatacaaa
     1381 agaatattga ccagacaata ctcaatgttt gatcaatgaa gatgatcgtt cttccaatat
     1441 gtttaaaagg atggagcatc acttttttga aattcgtcag ccagacttct gttccacctt
     1501 ttgcccaacg ttttctttgt cgatatagca tcttcaacgt cacaggtact tccatgaaaa
     1561 agataatctc aggagcaaaa acagataacc agtcatttaa ctgatgatcc caagcaatac
     1621 tgatgtcttc tgtcgctcga tcttgtctga atccgcccac atcgatcaag gcttcttttc
     1681 gatacatcgt atttgctcca ctgtacgcat aaagaccacc gaaaacagct gactgtgttc
     1741 gtttaatgat gcccacgata cttgagaatt caaccgtttg agattttgca atcaatttgg
     1801 tacgattttg gacatccata tttgcagtga ccgcagcaat atgtcttgca ccagggcgaa
     1861 tgaaataatt cacataccta atcagtgcat ctggctccgg taccgtgtct gcatcattgc
     1921 ttaaaatcaa tttccctttt gcaaaggcaa gtccgatatt aaacgcgtgg gcttttcctt
     1981 tatttttttc gatccgaacc acacgaagat tagcgtattt tttcatcagt ctagctaata
     2041 tttctggtgt ttggtccgtt gaaccatcgt ctgtcactaa tacctcatag ttgtgataat
     2101 tcaatttcgt catcaaatac tcgattgtat cttcgatcac aatttcttca ttatgggctg
     2161 gcaccatgat cgtgatgaac ggttcgacac tcaagggaac atctacccat tctttttgct
     2221 tatgtttgta taaaaacaca taacaccaga caccaataaa ccatacaaat ccgcctaaaa
     2281 tcggataaga aacgagaaaa aatacataat agacaaacaa gtatctaaga ataaattacc
     2341 tgtatgggtg attgagatca tttcagttcc accccatttt ctttatacaa atctgcaatg
     2401 aatgtcgtat ctagattttt ctcttcggag acagaataaa aacggaccgt ttctctctct
     2461 tcttttgttc caaaacgttc cgtatacact tcttcaagta attcttgtct ttttttcaat
     2521 ttttcatctt catattcaaa agatttattt acttttttgt atagatttcg attgttccac
     2581 ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt
     2641 aagaaataca gcattttctt ttctgataaa aaagcgtaca aggaaaaatc cttggctctt
     2701 tctggtctcg ttaatgggaa aaagacccat ataaaaggaa gaacaacgat catccatcct
     2761 aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat taccttttga aaaatactta
     2821 tctttcacca aaattgatgc aacttcttct cttgttactt ttttcatatc ctcacctcac
     2881 gtttttttgt tacgacgtag gagtttacca tacatttcat ttcttttcac ataaagtgtt
     2941 cctaaatttt tcctaagaaa aagaacaaaa catttctcat cagtaaatga aaaatatttt
     3001 gttcttttta tcagttgttc tttttatttt ttatctctta attatgttgc tctttccaat
     3061 acgacaatgc ttttcattcc tctagatatc gccgtgtaca tttgtctgcg tcttctttgc
     3121 tcatctccat cgttttcaaa tggatataag attacatgat caaattccag tccttttgct
     3181 agactcaaag gaatgatttg gcagttttct aatccatatg aatttttttt aatcgattct
     3241 tgtagcgttt gggtttcctc ttctgtttgg gtgataactg ccgctttttc tccttttaga
     3301 gaaaccaaaa tctcagctag tattttcaac aattcttcag aggatcgaaa agagcggtat
     3361 tcgggttctt ttccttgcgg tcggatcgat acgatgtcga tcttctcttg atccacagca
     3421 taggttttga aaagctctgt gattgctccg ctagaacggt aactatttct aagatcatac
     3481 cgatggatcg gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa
     3541 gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt tggaaataat
     3601 tcgatcaata gacctaattg tgccagggta taatcttgta cttcatcaat caatagatag
     3661 cgaaatgctg gtacatttac cttttcaaca agtaaatgac gaatagctaa tataatgact
     3721 gcttcatcaa cagtacgtgt gggttgccaa gcataagctg agccatatcg tttttcataa
     3781 attctttcaa acaactgatg ctcatccacc catgccattt cttcgatttg tctggttatt
     3841 tttcgatatt ttttttgtaa aagtttcctt gcataagcta caatactttg ttccgaatca
     3901 tcagaaatca atttaccgaa taattccaac tgcctatctt ctgtcaacga gagtacctga
     3961 ttctgcacag caggactttt tgcttgtttc aaaagatgat tcttccattc gctcatcagt
     4021 gcctttttcg ttgcttgaag cctatcgatc aatttaggat gagcaggaac tttctcatat
     4081 attttttcga tcgttttttt agaaaggact gtttttccat ttttagtaag tccacggata
     4141 aaattcggat gatctaaaaa caaaggatct gattgtttta gattatcgat aaactttttg
     4201 ctacgcaaac gttctgtctg ctccgataca ttttctcccg tgatacgttt gaaataagcc
     4261 tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat ggttagattc
     4321 agtggatttc tttcacctaa tgatggtagg acattcgaaa tatagtcgat aaaccgatga
     4381 ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa atagagcaga
     4441 tacgcaatac gctgcatgat agcagaggtt ttaccacttc ctgctacgcc atttaccaag
     4501 atattttgac ttttcacatc ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg
     4561 atagccttca tttcattggt ctcatcttgg cctaagactt ctaataatac gtcatcttga
     4621 atagctacag acgtatcaaa aaacttgacc aaacgatctc tttccaaaag aaattgtcgt
     4681 ctttctttaa taaccgtatc gattttattt tgacgaaccg tataagaaga tggaccaatc
     4741 atgttattgt aaaaaagttc ggcaattggt gaacgccaat cataaacaag attttcatct
     4801 gcatcatctg tgaaatttgc tgtaccgata taaaaggatt cttttggctc attatccaga
     4861 aagtctacaa cgacttttcc aaagtaggga cttttcaata aacgttcgat ctttgctagc
     4921 tgattttctg cgtttttctg tttgatattc atctgatcga tttctctatt tttcatttca
     4981 atcatcgaaa aagtatccag gttatctaag taactgtcaa aattcaatcg aagctcgcca
     5041 gccatttctt tcaacgactg gtttccttct atttttgccg tatccatcga ttcccgcagt
     5101 tcgttttttt tatcaattag cttttgatac acgttcttca gatatgcttc ttctaactgt
     5161 ttttgtgtgt tgaccataca cattctcctc tttttttaaa gtggtggaac cgattagttt
     5221 acctctttta agagtcgaat gcaagctttt taacgagtat catttgttag gatatatcag
     5281 ttcttcaggt cggacagttt gatataaaga aaatgcacca tcaaggtttt ttacgtggta
     5341 gccagactgc ttcaagattc tttccgctag atagctacgt aatccgctat gacagctgac
     5401 gatatattct tgttggctgt ctaattcatt taatcggtcc cgaagttcat tcaaagggat
     5461 atgtttggct tccttgaatt tgcccttttc caattcttct gctgttcgca catctaataa
     5521 aatttttcct tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt cactaagccc
     5581 ttctgcaatg ttcattgctg catatcctag catgtttact ggatctttgg ctgagccaaa
     5641 tggcggtgca tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat gccctttgat
     5701 tgctgttgct aaaatatcga tccgtttatc cacacctttt gccccaacac cttgtgcacc
     5761 ataaatctcg ccagtctctg gatggaagat caacttcagt aaaatatctg ttgcttctgg
     5821 ataatagctt gcatgatctt ttcctgatac atgaatgact tcaaaagaca gttcgttttc
     5881 tttggctgtt ctttcactta aaccggtgga tgcggcagtc attccaaacg ttcgtataat
     5941 tgctgttcca ataccgcctt gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg
     6001 tctaccttga cgattggctg gtgaagcaag agaaataagt gcatcttttc cagtgatttg
     6061 ctgttttaca acgatagcat cccccaccgc ataaatatca ggaatattcg tttgataacg
     6121 ttcgtctact aggatacccc cgcgtatgcc taattcgatt ccagcggtca ctgctagctg
     6181 gttatcagga cgaacaccaa tggataaaat aactaaatca gcagggatgg tacgcccatc
     6241 atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg acagcatgag aagtaatcac
     6301 ttctactcct tgatgagaca attctttttc tataaaagct gccatttctt catccagtgt
     6361 tggaagaata tgtggtgctt gttcgatcac catgacttct aaacctcttc tttttaggtt
     6421 ttctgccatt tccaatccga tgaatcctgc accaacgatc acggcacgct ttggctgttt
     6481 ttctaatgca tgcatcactt tatcgatatc tggtacattt ctaatagaaa aaacattgtc
     6541 ggcttctgtt atccctggaa tcgatggaac aactggtttt gcaccaggag ataaaaccaa
     6601 tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg acttctaccg ttttgttttc
     6661 gggaaagatg gctgtcactt catgatttgg acgcacatct aaacaaaaac gagcggataa
     6721 tgattctggc gtttggacaa gaagattttc ccgttcactg atttctcccg aaagataata
     6781 aggcaagccg caatttgcaa aagaaacata cggtcctttt tcaaaaacaa cgatttctgc
     6841 atcttccatt aatcggcgaa gccgtgtagc agcagacatt ccacctgcta cacctccgac
     6901 aataacgatt ttcatcttat ttccctccta ctgttcgatc aaaccattga ttcatgccac
     6961 cacgtacatt cactacatca taaccagatt tctttaattc tttagctgct tgtttactcc
     7021 tcatccctga ttgacaaatc acatagacgg tcttttcttt gtctccatca tatctgttta
     7081 tactttgtaa tggaacattt ttagctcctt ttatatgccc gccccggtat tctgcaggtg
     7141 ttctaacatc aagtaacgct ggattttcca aaagcttttc ctttaattct gggactgtta
     7201 tcgattttat tgattgaaat agcaaattca ttttctgcac ctcatttaca tttttataaa
     7261 tacctcatgg ggtatattaa acaggagctt tattaatatg tcaaccagaa tgactcttca
     7321 tttttttcac tactttttat atttgcataa aataccgata aggggtatta tctaattata
     7381 aaaatagagg agaatgtgtt atggagattg ataataaaaa tatcctgaac cgtttgaaac
     7441 gtacagaggg acaaattcgt ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg
     7501 atgtcatcac tcaattaagc gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg
     7561 ttgcagataa tctgaaaaat tgctttgaga aacctgatag taatccagaa gaacaagcgg
     7621 ctaaactaga acaagcgatc aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa
     7681 gtcgttttcc atattgatga attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac
     7741 ctgataaaag cttctccaga aacgacgatt gtcgtatctg taaatggcat cgcgattacc
     7801 ggttatctag actctgccaa tgcagaattc ttagatttgc aaggagtcgt ttttcacgct
     7861 tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt ctttacctga acaagtaatc
     7921 gttgtaccag caggtgtcct tgatttagtt gaactgcaat cccaaggtta tgcttatatc
     7981 aagccttagt cagtagaggt tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt
     8041 tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca
     8101 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcgttt ggttgggctt
     8161 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt
     8221 tgacctaagt attagagaaa aaaacgggaa aatagctcct catattttgc cgttttttga
     8281 gctaataccg aaggtcagct gattgaacac cgtttaagga agagattgtg aaaaagtcgc
     8341 ttagtttcga gtattaagaa aaattttgtg aaaatggatt tctacatttt tgcataaatt
     8401 tggcttaata ccgagaaact ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag
     8461 cggtttgacc tgagtattag agaaacaaac ggaaaaatag ctgctcatat tttgccgttt
     8521 tttgagctaa taccgaaggt cagctgattg aacaccgttt accaagaggt tgtgagccta
     8581 ccatttaact ccgaatcaca agaaataatt tcaaaaacaa cttctcctgt ttttggtttg
     8641 gttggcttat tatcgagaag tagctactgg gagtctattt atcaaaaaaa ggaatgcgac
     8701 ataggtcaca cccctttttt attttctttg aaacatcttt cggtactcga cagcatacaa
     8761 gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa atgactctca cccacagcgt
     8821 tggtaattct gggaaaagca gcaaaaggat catgccaaca tagatgacag cagtcgctac
     8881 tttgccatac cacttcgctc cacttaattt gatattttta gatagcccga ttaatccagc
     8941 tacaaacata aagccttctt ttatcaagaa tagaccaaac aacagccaaa gccacggtcg
     9001 ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag
     9061 caccttccct aagtctgtta cctgattata tctccgagca atgaccccgt ctagataatc
     9121 agacaaacct gagacaacaa tgaccgctcc tgctatataa aatgactgtc tcagcacaaa
     9181 ataaagataa atcggcagca aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg
     9241 tatttcttta cgccagtctg tgcttaattt cattttatcg ccctacctcc tcagcactct
     9301 tttcttttta ttttagctta cacggggaat aaaaccaatg atttcactgt ttttattttt
     9361 ctttacagcc actgatatta tgagctaatt tgagataatc acttgctgtt ttcatcgttt
     9421 actcttcttt cttcctgtcg tttgcgcatt tcttttacta gtactttatg agcttttttt
     9481 gatcctcgct ctaggtcacg ctgccgttta cgtgttttat aatcgataaa cagactgtct
     9541 aaatcgtaac ctgtcgggta cagatcttct gctgaacgca ccagacgaac acgctgacga
     9601 ggcacgtgga ttattttttt gtctaaatat acatcaatcg tatcttcacc tatatcttca
     9661 taaactaacg cttctttttg gtattcattg acaaatatcc gatcaccttt tgcaaagagc
     9721 tggcgttctt ttttattctc cttcctattt gtaactgctg tcttaggaag aaattctttt
     9781 ttctgtgtcc gataggcttt ttccgccaag taacgctctg cttgatggat caatttcatc
     9841 gacatatcca ttttatgagc aatccacaaa gcttggcttt ctcctgtttc tccaacccgc
     9901 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
     9961 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    10021 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    10081 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    10141 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    10201 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    10261 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    10321 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    10381 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    10441 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    10501 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    10561 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    10621 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    10681 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    10741 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    10801 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    10861 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    10921 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    10981 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    11041 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    11101 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    11161 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    11221 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    11281 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    11341 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    11401 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    11461 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    11521 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    11581 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    11641 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    11701 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    11761 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    11821 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    11881 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    11941 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    12001 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    12061 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    12121 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    12181 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    12241 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    12301 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    12361 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    12421 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    12481 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    12541 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    12601 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    12661 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    12721 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    12781 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    12841 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    12901 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    12961 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    13021 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    13081 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    13141 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    13201 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    13261 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    13321 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    13381 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    13441 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    13501 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    13561 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    13621 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    13681 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    13741 gtatcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    13801 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    13861 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    13921 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    13981 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    14041 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    14101 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    14161 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    14221 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    14281 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    14341 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    14401 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    14461 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    14521 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    14581 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    14641 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    14701 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    14761 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    14821 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    14881 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    14941 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    15001 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    15061 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    15121 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    15181 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    15241 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    15301 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    15361 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    15421 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    15481 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    15541 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    15601 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    15661 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    15721 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    15781 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    15841 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    15901 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    15961 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    16021 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    16081 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    16141 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    16201 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    16261 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    16321 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    16381 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    16441 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    16501 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    16561 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    16621 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    16681 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    16741 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    16801 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    16861 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    16921 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    16981 tagacgagca tcttaaactt taagctttcc tgcaataaaa acaatagaat tttttccaaa
    17041 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    17101 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    17161 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    17221 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    17281 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    17341 tagctcatgt tcttctaact gttcaaacgt ccatccttct tttactgcaa ttgtcagttg
    17401 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    17461 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    17521 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    17581 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    17641 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    17701 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    17761 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    17821 attcaatgtt aattgttctt cccacatctg attgtcgggg cgagaatgat tagccagcaa
    17881 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    17941 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    18001 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    18061 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    18121 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    18181 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    18241 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    18301 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    18361 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    18421 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    18481 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    18541 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    18601 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    18661 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    18721 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    18781 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    18841 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    18901 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    18961 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    19021 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    19081 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    19141 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    19201 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    19261 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    19321 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    19381 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    19441 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    19501 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    19561 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    19621 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttctt caaaaggttt
    19681 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    19741 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    19801 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    19861 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    19921 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    19981 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    20041 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    20101 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    20161 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    20221 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    20281 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    20341 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    20401 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    20461 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    20521 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    20581 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    20641 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    20701 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    20761 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    20821 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    20881 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    20941 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    21001 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    21061 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    21121 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    21181 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    21241 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    21301 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    21361 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    21421 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    21481 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    21541 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    21601 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    21661 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    21721 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    21781 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    21841 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    21901 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    21961 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc ttcgatagct
    22021 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    22081 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    22141 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    22201 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    22261 accccttcca aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    22321 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    22381 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    22441 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    22501 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    22561 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    22621 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    22681 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    22741 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    22801 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    22861 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    22921 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    22981 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    23041 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    23101 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    23161 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    23221 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    23281 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    23341 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    23401 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    23461 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    23521 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    23581 ccccgtttac aggactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    23641 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    23701 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    23761 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    23821 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    23881 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    23941 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    24001 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    24061 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    24121 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    24181 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    24241 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    24301 ctacaactaa taaagtaatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    24361 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    24421 cagctaaaat tgctggaata accgtacttg tatatcttgc tagaatcacg ggcatcccta
    24481 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    24541 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    24601 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    24661 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    24721 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    24781 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    24841 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    24901 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    24961 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    25021 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    25081 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    25141 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    25201 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    25261 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    25321 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    25381 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    25441 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    25501 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    25561 tctgataaat ctccgaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    25621 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    25681 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    25741 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    25801 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaaccaaat
    25861 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    25921 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    25981 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    26041 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    26101 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    26161 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    26221 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    26281 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    26341 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    26401 gcatgatatt ggaaaaaatt gtactgattt gttgaggcaa ccttgcgtgt tggatcaccg
    26461 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    26521 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    26581 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    26641 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    26701 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    26761 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    26821 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    26881 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    26941 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    27001 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    27061 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    27121 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    27181 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    27241 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    27301 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    27361 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    27421 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    27481 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    27541 ttatagacag cgctacgata ataactaaat gcaatataat ctaccgtgtt agcttgcaag
    27601 tactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tatcctgtaa
    27661 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    27721 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    27781 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    27841 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatctcttt ttttgttagt
    27901 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    27961 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    28021 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    28081 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    28141 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    28201 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    28261 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    28321 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    28381 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    28441 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    28501 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    28561 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    28621 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattta tatataacaa
    28681 atacataaga cgatccccga aagaaaacac tcgttaacca tctaagcaaa cgatttattt
    28741 tacttccaac acaaatgctt cataaggtcg taaatcaact ttgccgtctg gtaactgttt
    28801 cagaggataa tttgacacaa taacccttaa agcttcacgt ggaacagtga acgtctgctt
    28861 ttcactggac atattacaaa taacgagcca ttgctggtct tcatatatgc gagaataggc
    28921 aaagacatgc tcaggcgtct tttccactaa ttcatagtct ccccagacga tgatgtcatg
    28981 ttttctacgc aaatcgatca atttcttgta catataaaaa acagaatctt gatctgctaa
    29041 cgcttcttct gcattgattt ctttataatt tggattgact cgaagccatg gaattccagt
    29101 agtaaaccca gcatgttcac gggcgttcca ttgcataggc gtcctagcat tgtctcttcc
    29161 tttcgcattg attgattcca agatttcctc ttttgtaaat ccagaagaaa tcctttcgtt
    29221 atacatattg attgtctcaa tatcttttgc ttccgcgata tcatcgattg ggcaattcgt
    29281 catgcctaac tcttctcctt gatagatata aggggtgcct ttcatcatat ggagcaagat
    29341 agcaaacatc ttggcacttt cttttcgata cacatcgtca tttccccaac gagaaacaat
    29401 tcgcggaaga tcgtgattat tccaaaataa actattccat ccttcatcac ctaaagatgt
    29461 ttgccatttg gataatactt ttttcagttc agagatactc aagggtttca aatcccattt
    29521 gtctttgcct tcttgctgat ctaatcctat gtgttcgaat tggaagacca tggataattc
    29581 atttctttta ggattggaat aaagtttagc gatttcggta gttgctcccc atgtttcgcc
    29641 aacagtcatc agatttttgt caccaaaagt tgcttgattc atttcctgaa gatactcatg
    29701 cagtttaggt ccattacttg tgatttcttt atccggttct ttgccaatca tatcgatcac
    29761 gtccatacga aagccaccaa tacctttatc gatccagaaa ttcatcattt gataaatctc
    29821 ttggcgaacc tgtttatttt tccaattcaa atcaggttga cgcttgctat acaagtgcag
    29881 aaaatattgc cccgtttttt catccaactg ccaagcagaa ccactgaata ttgagcgcaa
    29941 tctattaggc tcttttccat tgaccggatc acgccaaata taatagtccc gataaggatt
    30001 ttctttattt ttacgggcct caatgaacca cggatgttca tcagaagtat gattcaccac
    30061 cagatccatg atgatacgga tatttctttt ctttgcttct tcaatcagtt cttccatttc
    30121 ttccatcgta ccgaattctg ccatgattgc ttgataatta ctgatatcat acccattgtc
    30181 atcattcgga gactcgtata ctggagaaag ccaaattgca tcaataccta aatacgccag
    30241 ataatccaac ctggaaataa tcccccgcaa atctccaata ccatcttgat ttgaatcttg
    30301 aaaacttctt ggatagattt gataaacaac tgaatggtgc caccattttt gttccatgat
    30361 tttcctccta taaatggatg tttctatttt ttgcttaatc atttatttta gtctctctca
    30421 ctaaaaattg taacgctttc aaatagctat tgcaccttgt tatcggtaac agaataaaac
    30481 aaaaaaacag ctgaggaaat tccctcaact gttttttgaa aggattgatt tattcaggga
    30541 agaatgctga tttcacaact tctttataaa cggtgttcac tcagctgtcc ctcggtatta
    30601 gctcaaaaaa cggcaaaata taagaaacta tttttttcgt ttttttctct aatactcagg
    30661 ccagaacact gatttcacaa cctctttata aacggtgttc actcagctgt cccttggtat
    30721 tagctcaaaa aacggcaaaa tatgaggaac tattttttcg tttttttctc taatactcag
    30781 gccagaacac tgatttcaca acctctttat ttcacattca atatccacat gaatcccatg
    30841 aatacaaaga acagaccata catgatggga gatacttctt ttcctctttt agctgctaac
    30901 atggtaattg gatagaagat aaaacctaat gcaatcccat ctgaaatact ataagtcaaa
    30961 ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct ctaagtttcc ccattcgatt
    31021 tgtcgcaaag attgtgccat caatactcct accataatca atgctggtgc agttacttgt
    31081 gaagtaacaa ctgatagtaa tggagaaaag aacaagccta acaagaagaa aatccctgta
    31141 gtaatagctg tgatacctga acgtcctcca acggcgatac cagctgatga ttctacaaat
    31201 gcaccaacag gagaagtacc taataaggaa cccgcaagca tagctgttga atcagaagct
    31261 aaagctttcc ctacacgagg catcttgttg tctttcatga aacctgcttg gtttgctaat
    31321 ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca agaatgtcag cactacgacc
    31381 cacatttgaa gagaattgat gtcccctaca tgttttaatg caactaaaaa tgttggtttc
    31441 aaacttggtg ctgcggcaac aatttcagaa ggcattggaa tcaaacctgt gaccagacct
    31501 aaaatcgttg tagctgccat cccgatgaag atgccacctg gtacacgccg aacaagcaag
    31561 atagcagtta cgactaatcc aaaaatcgtc aaccaagtag aaccaacgtt gagtgatcct
    31621 aatcctacta atgtagattc attcgaaacg atgatcccac cttcgctcag accaaggaaa
    31681 gcaataaata gaccgatacc gccagaaatc gcatatttca aatctgctgg gatcgcatca
    31741 atgatcattt cccgcagctt gaagattgtg atcaaaataa aaatcaaaga agcaacgaat
    31801 acgccggcta atgctgtttc ccatggaatc cccattccta aacaaacaga ataagcaaaa
    31861 aaagcattga tcccaagagc aggtgccgtt ccaattggat aacgggctaa tacccccatt
    31921 aaaatacagc cgatcgcact ggctaatgca gtcgcagtaa acactgctcc ttcatccatt
    31981 ccagaagcac ctaatacagt tggatttaca aataagatat aagccattga gataaatgtt
    32041 gtaaatcctg ccagtacctc acgttttacc gtggtgttca atttttctat ttcgaagtag
    32101 gaaagtattt tttccttcat cttccatccc cctaaatccg aacattataa ataaagtccg
    32161 ttaaaatttc gatttattaa taaaacaacg tgttttatca tatactaaaa caaaacataa
    32221 gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt ccatctgatt aaatcccttt
    32281 atctcaaagt tcatgaacat tcactttgga ttacaagaat ttctttattg tgttaataaa
    32341 gtttcttcat tctagtctca aaaatcaata gtgtgatttg attttaccat ttcgtttctg
    32401 ttcttctttg atgaattctt ttgtatcgac aggattcaga ttatttagtc cgccaaaacc
    32461 tgtatcgacc atttttcccc agccttttgt cttctttttt cctgatttga gattctttgc
    32521 tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa taaccttcta atcttcccat
    32581 tagatccact gtttctcctc ctttttattc aatcagacga ctcagctttt cctataacaa
    32641 aataattgcc ttcagggtcc gaaaaattga aagtcaagac gccattgttg tctgtgattt
    32701 ctcctggttc ttccatgttc tcgtagatag tttcaaattc atccgtaaaa aatactaaag
    32761 aaggcggtgg tcctaatacc tctggtgaaa acttctgcac aaatactttt gggaatacgc
    32821 ctaattctaa ttcttttgag atgctgagta caatactctt aaattcttct ggcaattcga
    32881 ttgtttcaga aatttctgca ccaatttttt ctacaaagaa tcggcaaatc atctctacgt
    32941 cattgacata aagcatcaca cgtgtttttt gtatcatcgt ttgacccttc tttctatttg
    33001 attttctttc ctgtctataa actgattcca ttatatcacg aaagcgcaaa caccttgtgt
    33061 ttgatactat tcaagaaaaa taaagcaata gtttctagga ttgctttcct ccacaaactt
    33121 ttttta
//
LOCUS       DAXLPI010000019        30468 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXLPI010000019 DAXLPI010000000
VERSION     DAXLPI010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.019"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..246)
                     /locus_tag="ACF3T5_000776"
     CDS             complement(<1..246)
                     /locus_tag="ACF3T5_000776"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3631407.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEE"
     gene            complement(248..2263)
                     /locus_tag="ACF3T5_000777"
     CDS             complement(248..2263)
                     /locus_tag="ACF3T5_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFD3631408.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(2442..2747)
                     /locus_tag="ACF3T5_000778"
     CDS             complement(2442..2747)
                     /locus_tag="ACF3T5_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFD3631409.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(2836..3867)
                     /locus_tag="ACF3T5_000779"
     CDS             complement(2836..3867)
                     /locus_tag="ACF3T5_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFD3631410.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            4074..4949
                     /locus_tag="ACF3T5_000780"
     CDS             4074..4949
                     /locus_tag="ACF3T5_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFD3631411.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(5101..5943)
                     /locus_tag="ACF3T5_000781"
     CDS             complement(5101..5943)
                     /locus_tag="ACF3T5_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFD3631412.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(6035..7912)
                     /locus_tag="ACF3T5_000782"
     CDS             complement(6035..7912)
                     /locus_tag="ACF3T5_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFD3631413.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(7909..9330)
                     /locus_tag="ACF3T5_000783"
     CDS             complement(7909..9330)
                     /locus_tag="ACF3T5_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFD3631414.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(9333..12722)
                     /locus_tag="ACF3T5_000784"
     CDS             complement(9333..12722)
                     /locus_tag="ACF3T5_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFD3631415.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            12979..14370
                     /locus_tag="ACF3T5_000785"
     CDS             12979..14370
                     /locus_tag="ACF3T5_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3631416.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(14443..15090)
                     /locus_tag="ACF3T5_000786"
     CDS             complement(14443..15090)
                     /locus_tag="ACF3T5_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3631417.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(15098..15784)
                     /locus_tag="ACF3T5_000787"
     CDS             complement(15098..15784)
                     /locus_tag="ACF3T5_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631418.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(15790..16863)
                     /locus_tag="ACF3T5_000788"
     CDS             complement(15790..16863)
                     /locus_tag="ACF3T5_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631419.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            17085..17759
                     /locus_tag="ACF3T5_000789"
     CDS             17085..17759
                     /locus_tag="ACF3T5_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD3631420.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            17764..19104
                     /locus_tag="ACF3T5_000790"
     CDS             17764..19104
                     /locus_tag="ACF3T5_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFD3631421.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(19253..20239)
                     /locus_tag="ACF3T5_000791"
     CDS             complement(19253..20239)
                     /locus_tag="ACF3T5_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFD3631422.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            20963..21994
                     /locus_tag="ACF3T5_000792"
     CDS             20963..21994
                     /locus_tag="ACF3T5_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3631423.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            22103..23635
                     /locus_tag="ACF3T5_000793"
     CDS             22103..23635
                     /locus_tag="ACF3T5_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFD3631424.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            23774..24586
                     /locus_tag="ACF3T5_000794"
     CDS             23774..24586
                     /locus_tag="ACF3T5_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFD3631425.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            24576..25061
                     /locus_tag="ACF3T5_000795"
     CDS             24576..25061
                     /locus_tag="ACF3T5_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFD3631426.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            25084..25629
                     /locus_tag="ACF3T5_000796"
     CDS             25084..25629
                     /locus_tag="ACF3T5_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFD3631427.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(25702..27345)
                     /locus_tag="ACF3T5_000797"
     CDS             complement(25702..27345)
                     /locus_tag="ACF3T5_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFD3631428.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(27474..29702)
                     /locus_tag="ACF3T5_000798"
     CDS             complement(27474..29702)
                     /locus_tag="ACF3T5_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3631429.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(29735..30214)
                     /locus_tag="ACF3T5_000799"
     CDS             complement(29735..30214)
                     /locus_tag="ACF3T5_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFD3631430.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         8737 a         5784 c         5557 g        10390 t
ORIGIN      
        1 ttcttcgctg tcaatgatcg attgcaaagg tacgagcttt tcatcaggta tagaagttag
       61 cgtatctttc aaagaatgct caatgaagaa ataatcagcc atatcgctag atacacttcc
      121 aacatccgta tgacttactt caaactcgtc cacatctgct ccaagatctg caagtgcttg
      181 tttgatgttc atctctacca taaaacttgt tcctaatcca ctttgacata ctgcaaaaat
      241 tttcatatca ttcgtctcct tttcttatga tcgctaatat ttcttctttt gttgatgcgt
      301 gtaataaatc ttgaagattt tgtttattag aaagtagctt tgttaaattt gccaaagccc
      361 gaagatgtgc ctcattatcg atagcagcta aacagatgaa caaactaatg ggatgggctg
      421 gattatcaag cagatccaca ggttgttgaa gtttcagcaa agacatccct acttcgttta
      481 cgccatcttc tggacgagca tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac
      541 catactgctc cacgcgctcg atcatagctt tgatataact ttcttctatt ttattttctg
      601 ataatagcgg ctgtgcggca aatgtgattg cttcttgcca gttttttggt tgatccgata
      661 attggatcat ttcttctgtt aataattcag atagcatagg acgttgatcc tccttctttt
      721 ctaaaagttt gttcatttta cgatttaata cttgatagag cttttcttcg tctacgcctt
      781 cttttaattc gatataaggc agtagcgccg acaacaattc ttgggcagaa ggcattgaaa
      841 atcctggtat cagccaatct ctttgtactt gattgatcag ctgatttttt tccaagtcag
      901 tcatgattgg atgcagaaca tagagagact ttttcttatc agtaagtgtg agaggtacag
      961 tagaaaaaat aatatcgtaa gaagtttcgt caacctcgtg taagcggtca gctgaattag
     1021 tctctgtaaa aaagatcatc gggaataatt gctgaagttc actttttaag atcaatgaag
     1081 agctaatgcc acttggacag acgatcacgg ctcttatttt tctatttctt tcttctgcgt
     1141 ccgcttttct gatctctccg ccaaatagta tagtgaaaaa accaatctct tcttcaggga
     1201 tgctcttccc tattcgtctt tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg
     1261 aagcatattg atgcttaatt tgatcgatca tcacattagg taaataaaaa gaataggaaa
     1321 tacgaaaata ggcagggacc aagtgataaa agacattcat caataattca cgaaacgaag
     1381 caaattctac ggccatcagg aattccatgc gatggatgac ttctgaagaa cattccagca
     1441 aaaaatcaag tgcaggctcc tggacatctc cttcaacgat cgatgctaag caaagagaga
     1501 aaaatataat ctccgaattt tgatgttcgg aatagccaag gccagtcaat acttgtcgac
     1561 taatgtcagt gactgcaaga gaagccaaca gttcagcatc ttttaccagt tccatggatg
     1621 tttttccaat tcgaatggtt gtcaaactca aataataagc taattcatcc attcgatttg
     1681 gtgcgaatcg aagatctgat agtgaaatcg ttttatctag caaatctcta actttggcgt
     1741 aataatcaat ggaaagggtt gcggttaaat acgtgagccc aaatcttcca gcaagtgact
     1801 gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa tattgcttct gaccctagca
     1861 gcctaaaacc agttttccta gtatacgaca gctgacaatc ttccacttct aattcatttc
     1921 ttatcttttt gatatctgac aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat
     1981 gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct
     2041 cttgttcggt atacacagct tcaaatcgag taatatccaa aaatttttct gcccagtcga
     2101 ttgctcggaa tttctttata cggatacggg tatcatccac atattcggca aaaggagtta
     2161 attgtttgat cgcctcgctt gtatttactt cgatgatctc tgctgctttt tctagactga
     2221 tttctcttgg tgttttcagg agataactaa ttttgtagtt cacagcgctg ttcacctcct
     2281 acttatattc tagtatggta gcgcttttaa atcgaggggt gtttggtaca acttacagtt
     2341 ggattttttt tacgactgta ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg
     2401 tgaccaaatg tctcctgctt ttatgaattg atttcgtccg atcaatgatg atcatgttta
     2461 ttttttgagg tcagttcttc aaatttttct ttgacatctt caaccgcttt ttcgatatct
     2521 ttttcaaagt gattgggctc attaggcttt gtttcctctt ctacagaatt tgaaccagat
     2581 aaagctgttt ctttattttc tgtttcacca gagtttgtgc cagctaatga ttcttctttg
     2641 tcttctgttt caccagaatt agttcctgct aaagccactt cttcttctgt tttaggggcc
     2701 attgaacctc ttaatcgttt gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct
     2761 agtagttcta tactttttca tcgtacaata agacagttta tgcgtcaaaa aacaaacccg
     2821 attcttacaa atatgtcata tttttccttc tgattcttga atgcttttga ttccttcttc
     2881 caaaattttc atttgtaaga ttgtttcttc atctttgatg actttttctg cgccattaac
     2941 taatgtctcg taaatccctt cgtagacacg gctgtaatca ccaacctcgg aaattacttt
     3001 ttcttcatga taactccctt gttcatctat ataggtcaat acaccataat gttcgggcaa
     3061 atcgatacca aagtcttcat gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg
     3121 atcttttgtt tgtttcacga acatcccttt ttttccgtaa acgacgaaac ttggacgttc
     3181 tttgatgcgg aaataactgg attttatcga aattttcata tttccataga aaaaatccag
     3241 atcgaagtaa tcattcattc gtcctttccc taaaagttga cgtacatcat agctgatatc
     3301 atctggttcg ccgaaataag aaagaacttg atctactgta tgacaagcat gtccatataa
     3361 atagctgttc gcagtagaaa attcatgcgt attttctggt acttccggtc tgaaataatc
     3421 ataatgcatt tctacttcta aaatatcccc taattttccg ctttcgatta ctttttggac
     3481 tgtcaagaag tcagaatcga accgtctgtt ttggtaacat tggataaata gacctttttc
     3541 ttttgctaat gcaaataact cttctgcttc ctttgacgtt tctgcaaaag gtttctctac
     3601 tagtacattc ttaccatgtt cgagtgccat ttttccatat tcataatgaa attgactagg
     3661 tgtagtgatg acaactaatt ggatctcagg atcatcccaa agatcagaca gctgatcagt
     3721 atattcgatg tcttcgaatg ttttccacac tggttcagat ggggaaaaaa tttttttgat
     3781 attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga tagcgatttg tactctttcc
     3841 attgccaaga taagcgattg taagcatgag tggtgcctcc ttttattgat gttttcagta
     3901 tatcctagaa aagcgcagaa agtatttata atagctattc tggtaaaaaa gttcagaaaa
     3961 acacttgtta tcattcggtt tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa
     4021 gatagtagcg gaaccaagtg tttatcagta agatagaatt tggaggtcag atcatgctta
     4081 ttgaagaaaa actaacaaaa caagagttgt ttaccacgac agagaaacgg attgtcgatt
     4141 acatccgtag gaatatcgaa gctgcagtct acatgacgat tgaagaatta gcaaaagcta
     4201 cgtatacttc ccattcagct atcattcgtt tatgcaaaaa aatgggattc agcggattta
     4261 aagaattccg tttcgagctg gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa
     4321 cgattgatcc taattttcct ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa
     4381 tggcggattt atcgatccag tcaataaaaa aagcacaatt gcagattgaa catgaagatt
     4441 tagatcaaat cggtaaagta ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg
     4501 attctcaaat cacggcaagg aaattccaga ataaaatggt caagttgaat aagtttttga
     4561 tcatggctga agaatacagt gattcttctt ggaatgcagc caatttgact agcgaggatt
     4621 gcgccatttt tattagctac agcggtcgca tccatcatta tgaacgaata atgacctatt
     4681 tgaagcatgt agaggcccct accttgctga ttaccggaaa tcagcattca gaaatggcca
     4741 aacaggctga tatgtgtctt gttatatcgc aggaggaata tgattttgct aaagtagcga
     4801 ctttctcctc tcaaattgcc ttcgattacg tattaaatac gttattctca gtgatatatt
     4861 ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa acaagaattg atgcaaaaag
     4921 gccttcttaa agaatatcgg aaaacttaag aaaagatcct catttagtaa taagaaagat
     4981 agtgtttgat aggctctctt tgatatcatc ataaaaacag gctgaagttt tctttttcaa
     5041 acctcagcct gtttgtttac ggaacgattc gaatagatat attatagaaa tgaggaatag
     5101 ctaacttctg tttcttattt tgaacgattg acgaaaaaga tatataagaa ggaggaaaat
     5161 cagtatacat gcagctaaca atccatacag gaaaaatcgt ttgttctcgg atactttctg
     5221 tacttctttt tgaacttctt ttgcatgata aggaacccgg tgccctctga caagtaagcg
     5281 atgactgttg accatataag gtgtacaagt taggagtgtc acgaaatcct gccctttttc
     5341 gattctcaga tcttttgtat cagtcggttc taccgtcttg atctgatcga cttgataagc
     5401 gagtgtccgt ccattgattt cgatatagaa agaatcacct aaagtcagtt tcggtaagtc
     5461 tgtgaacaac tttgcttgag gcagtcctcg atggctggaa ataacggcat gtgtattttc
     5521 tccccccgta ggataagacg ttccttccaa taatgaggca cctttttcta gcagcacagt
     5581 attcgtttca tcaaaaattg gcaaatggac attgatctca gggatcgtca aaataccgat
     5641 cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt tcttttttct tagtaaaggg
     5701 atctgcacct ggatttccac cttcttttgc taattgctgg tttttctttg tcatgttttc
     5761 ttggatttta gccatgactt cttcattttc tttgaccgct tgttgctgat aatgagagat
     5821 gatctgctga tccagataat tattcagcgc gtcgctaaca aatggataag ctagtatccc
     5881 tattcctgag agaagcaaga aaagcatcag cagatctaga agtttccttt tctttggttt
     5941 catttgtgtt ctccttgtga tgactgtttg ataataaaag aaaaaggagc atttgcgtaa
     6001 tccgctgctc ctttttccaa ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag
     6061 caaagtacag tccagcaatc agtagaagga ctactcctgc accgatatac acatagattc
     6121 ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt tggtattttt tcaggagaaa
     6181 tcaactgtcc tgctgttaca taagattgtt catcgatagt aaatgctaca cggtttgtta
     6241 atggaacata tttttctggc gctttcgttt cttccagata gtacgtgcca tattttagac
     6301 ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg
     6361 atacccagct gacggctttt gtggatgggt cgatcgataa atatttcgct gtgtcactat
     6421 cttgatcacg aacgacgaat tctgctccag caagtgtttg gtctgatgta acgtcaccat
     6481 ctactttaac aaatcgtttg cccccagtaa cgacatcgac tgacggtggt gtttgatcat
     6541 ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc tttggttgga tctgcttttt
     6601 cgttcaaatg catatagtaa acgaatttca atgtaccgcc aggagttaat gaaggaatat
     6661 aattcggatc aactgaaacc gtgaatccgt ctgtttgctc agtgacagaa taatttactg
     6721 ggtcgatttc tttatttcca tcatataagg cataagcata cgttcctgaa gaatcattat
     6781 caaatgttaa agcagcgtca tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg
     6841 ttccttcttt atcagcgata cctaatggga tattgacaga aatctcatat tgaatttttt
     6901 caccgattgc gacatctttt ccattcaatt gaggtgttgt tttatcaact ttagacgtat
     6961 catttttgat ggtcttttct actggtgttt ggctatttgc caggatctca aaaggcgtga
     7021 ttgtttgctt ttcaatcatt tccgcattat caggagcttt tacttcttct aaattatatt
     7081 ttccaacttc taaatgatta acgatcaact ggcctttttc aatagatgcc tcggcaaagt
     7141 cattgttgcc gatgtcataa gaatgaccag taatgaaatg tttggctttg gtttgatcag
     7201 ttgtccaagt gtacaatcca tctgtgacac tttggatgta ttttttgacg cttggtgttc
     7261 cttcttcttt agaaataata aattctgctc catttagtgc ttcgttttcg gcagtaccga
     7321 tttttgtaac tttcaacgtt ccatcattac cgactgtatt tttagggtag agatggatag
     7381 tatctagttc ttctgtcccg tatttataag agccatctgc ttgtttgatc atctcatata
     7441 cagggaaagc taaaaccatg tttgcggcag ctgacactcc gtcttttggt tcttctttga
     7501 tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa agataaagtg acatttccat
     7561 cagcatctgt cgttcctgaa gcaactggtg taccaggagt caaagactgg actgcttgtt
     7621 ttgctgcatc cacggacgct cctttatctc gttgcgcata aaattcttga gtgacgttat
     7681 aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc gaattcgctc atttctttcc
     7741 cgctgttttg gattaaagga tcgggtaaat cagtcatttt ctttttatga aggatgactt
     7801 gagctgctgt ctcctctgca aacacttttt ttgcgccgaa gctgtttgtg agtaatggta
     7861 aaataaggaa aaggactcct aacataacgt ttattttttt atgatttttc attttttcac
     7921 tcctgttctt tttttgataa acaaccagat tgcaaagctt gcgaatacca taccaacaac
     7981 gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga aatggacggc tattttcttt
     8041 tttattgact actttcaata tactgtccgc ctctttatcc tcagaaagaa aagttgaaat
     8101 ggtaaacgga atttctgttt cacttaaaat ataaccagaa ggtgcttcga tctcttttaa
     8161 tcgataactt cctgttttca acccggaaat tgaaaaagcg ccatttttat cggaaatcag
     8221 gtgaagcgca tctgattcgt tctttgtcca cttgtatccg ttggctgttt gctggaggta
     8281 atttcctgct ttgtttttaa taaggaatac tgcgccagct aatcgtttat ctggtttatt
     8341 tgtctcgatt ttgacaaatt gcttactccc tgtatataca gagactttct ttgtactgac
     8401 accaaaacca aaatccagtg ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact
     8461 gcttagctgc atctgataag aaatggtcag tttttttcca gcatattttt ccaacgaagg
     8521 gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt atgacaaagc cttctgtcag
     8581 cttttctcca ttcgatgaaa tcgttaaact attaggtaaa aacgttaatg cttcatccgc
     8641 actatctgac aattcgaact tttgatagtc aaggatattt acaggaattg tcgtatgcaa
     8701 ggaataagtg attttttcac caatcgtaaa atcatttcta ggctcttcta ataccttctc
     8761 aaaaggtgga tcagggtagt cgttttcttc atttttagga tacaaatgga tagttgaaag
     8821 tttttgattg ttttgtccat gtacaggaag aacaactacc atattctctg ccttttcttt
     8881 gacgacttct ggtactttgg attcaatgaa aagatacgct ttatctcttt tttcttgatc
     8941 tttagcggcc agagaaaaag aagcgatacc atcttcgttg ttaagagttg tagttgttgc
     9001 ttctgcggta aacataccgg aagacgcacc gttttttgcg atctctgctt gtgcttcttc
     9061 taccgttttg cccttttccc gtagatggta aaaagaatct gtgacatcat aaacttggaa
     9121 tgtgacacca tttaatcctc gatacgtttg taataaagct ttttcttgtc cgtcatttgg
     9181 atgatttttc ggcagttgcc cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat
     9241 ttctgtttct gtttctgttg ctacctgagt aaaagctggt ttaaatagta acaagaagag
     9301 acacatacta agccaaccaa gttttttcat atttagctgc cccctctttc ttttttcaaa
     9361 aaaagatacc aaatagaaaa cgcacaacca atcatcccta ctagatagat tcctaaacgg
     9421 ccaattcctc ccgtttcagg taatggtact tttgcctgat tcgtgatgtc taaagaaatc
     9481 tggttgtttt tggctcctgg tgacagaaca gattcatgat cttgtccatc cacttgaatt
     9541 gacccatctt cgtgtataac tatagtaact ggctctttta gaccttggta tccttctggt
     9601 gtaaaagttt cggtcagcgt ataagttcca ggttttaaat tttcgaatag aaaggtatct
     9661 gtttcttgtc catcttttgg cgattctaag tccatttctg gtccctgcag tctgaatttt
     9721 gctcctttta atgtctgtcc gttgtcttct tttttgtgta ccgttagatc aaacgctttt
     9781 agtcgatttt gatgagtgag cgtccataca ttatttgtca tctcggtatt ttttcccgtg
     9841 taaatgattt ttccatattt atcgatttgg aattcataat ttcccggcga agtatcatat
     9901 ccggcaggac cagctgtttc aacgactcga tatttaccag gtgtgagtga atcaaaactg
     9961 gcaagacctt tttcattcgt tgtttggctg tcaattggct gataagtacc atttgcttct
    10021 tttttctgta gggaaaatgc tgctccttta agatttatct ctttgtccgt cccttgcatc
    10081 gcatacttac gtactgctac aggaacttca acaaaaggat tttcaatagt caactggatc
    10141 gtatcatcgg aagtagtgac tgtttttcca tcaatggtta ccgtaccatc agaagcgatc
    10201 ttgatttccc aagtagtctt tttgcttaat ccatgccctt ctggagcttt tgtttctgtc
    10261 agcgtatagg tcatttcttt ttgcaatttg acattttctg gaagagaata ggttccgtcg
    10321 ccatgatctg ttagtaaagt atcaatagaa tctcctgtta atttgaaaac agcgccaata
    10381 agatcctttt cacctgtaga ggaatttttc gttattttca agtttaacgg tgtgaattgt
    10441 ttagtatttt tccatgtcat tgcatctatt tgttgagaat cgtatcctgg tacaggtaat
    10501 tctttgattg tttggtaact gaatgcttga ccttgattat tgtattgagg tagtgataat
    10561 atctcttgat aactttctcc gctatttgca gataatttgt caatgtctgc acgttcccac
    10621 gtatttgttg tatctttagc tggtttaatg attcgaatat atccgttttt ccaagctgca
    10681 gcatttgtcg tatgttcccg ttgaatctca aaagtaactt gatctggacg atcagctaga
    10741 ttgttgtcaa attcttccca taactttttg atgtgaagac tgactccggg agctttagca
    10801 gatggtatac cgaattcagc taattcattg gtatcaatgt ttggctggaa agttgtcctg
    10861 ccgttcattt gataccagaa atttggatgg aagtcctcat tttctgtttg gattctcact
    10921 tggtaatgga tttggatttc ttggtctttt cctaaataga tctgattgct cttgatggta
    10981 tttccttcta tggaaataga tggagtgacc gttgtaggac ttgtccccac actcttgact
    11041 gaaagtgtac caggctgata aatgaatggt tctgcgattg gatcatttat ttgtccattg
    11101 cttacagttg ctgagatctg tacagctttt ttggctaggt attcagagat atctgttgca
    11161 tgatcagctg attcatagta aagatcgcct ttttcatctg atgaaaccat ttgtcgcata
    11221 cgagactcta cttctgcttt tgagagacca gctgccggat cgctttgaag ttggatgcca
    11281 agaccatgta tttcgattcc tcgttctttg agtgccatcg cttctccgat ggttgcgata
    11341 aacgtactgt cgattcttct ggatagattg ttttggtcag gtgcatcata gattcttgag
    11401 attagagaag tatttcccgg ccgatcttgc gtattagaaa actgagttcc gtaataattg
    11461 ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa atgttggtac accatccgtc
    11521 agcaaaacga tcactttttt atgtccattt ggaacggata gcatgcttcc tgcatctctt
    11581 agtgcttttt gtgtaaaagt accaccattt gtccgtgaag gtgtaatgga ttttacttga
    11641 tttttcactg aatcaaatga acccatctgt actgcaccgt tactgtagct atatccttcg
    11701 cttgagtagc cgacatatcc catattgatt ttgtctgtga taccgctatc tgctaaagta
    11761 tcgacaaaac gatcgacacc aatctttact tcaccgatcc gattattgtc gttcatactt
    11821 cctgaccagt ctacgaccaa tacgagatca agaggcgtga tatctttttg tacatttcca
    11881 cgagcattca aataaacatc aaacaatccg ggtgtacttg tttcctttgc atactttcgt
    11941 atcgcatagt ctgcttgatt accggttcct ccatattcga tatacgaatg ggtccgatca
    12001 ttaggatctc cattccaaga atttatacca tcccattgat ttgttccgtt tttattgcct
    12061 tgatgatcaa gaacatttgt atttcctgta ggttgccaac tggctttagg ataagtgccc
    12121 tgatcatctg ttgtatattc tgtaggaatc gtttgattag atggcacact cacagctggc
    12181 aaggataatg taggcaatgt aaatgggctg acagaatttt cgttttcttt tgatgattct
    12241 tcttctattt cgtctggctt tggtactgac acatctgtat ctttcttatt ttctgtctca
    12301 tcaataatgt catattcttt attatcgacg atcagcttgt ttgcctggct gtggtaaagt
    12361 gctggatcga ttggaaaact cagatcgata gtttgtttag tgtttgatga gatttccaac
    12421 gtaattttcc cgctatttac ctgataagca tagccatctc cttcttttct ttccacatca
    12481 cttaagtaat tttcgactag accaatttct ttttctatct tttcagcact ggtgttattc
    12541 gcatttagct tgatgttcag tgcttttttt ccagactgat ccgaaatgac ttcagctgaa
    12601 attgtttcta aaactgttgg atcatctgta gtgtctgcta ttgcagataa tggcacaaag
    12661 tttgataaac tcaatattat cagcatgaaa ataactttca gtttcttccc tgtggtcgtc
    12721 atttttcccc tcctttttct ttttttctgt tttgacgtga atagtgaaaa ttattaaaat
    12781 aaacaggcgt tacataattt tatttatttt aatatgataa ttattatacg aagggcgcta
    12841 aagggaatca caccaatttt cataccaatt ggtttcattt atgaagttga ttccttcgat
    12901 taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa aacatatgat caggttgttg
    12961 gaattaaact atagaaagat ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg
    13021 atgactaaaa aagaacttgc caaacgaatt ggtagttcag aatctacatt tgtaaggtat
    13081 atcgaagaga tcaaaatccg ctggggaacg gatgtaacga ttcaatcttc acaaaaattg
    13141 gggtatcgtc ttgaacattt gaatgtacca gcttatttaa atgttctgat ggaaatgtcg
    13201 caaagttcta caaacagtcg tttattaaat gagattattg tcaatccggg taaaacgatt
    13261 gagtattatt gtgaaaaaat ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa
    13321 tgcaatcaaa ttttagaaag ctatttatta aaagtgatcg ttgaccaagg ttttcagtta
    13381 actagcctta aaagtgagct ccagttgcgt atctttgtta cctttttctt tttaagttac
    13441 tacggaagac gcacgttgcc atttccaatc gaaaaaaaga agatcaaaga aattatggtc
    13501 aagaataatt gtcagatgaa tttgtttcag gaagaaagcc catatgagca atctttcttt
    13561 attatgtatt ttgttgtgca tctcatgaga gaatcccaag gaactccatt tgaatggaaa
    13621 gctggagact cttatgtaca gttgataaat atcgataaaa aagacttctt aatcttgaaa
    13681 aatctcttag gaaaattgaa gtacagcagc tatataaaag tagtgaacta ttttgtcaat
    13741 acgatttttt cagaattttc ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa
    13801 aatatgtcct actgttcgtt ttcatccgct gattgcaaag aaaaaaacga agaatatgtt
    13861 gttcaattat taacgcaaat ttattttttc tcccgtattt tcccatacga tacgactcat
    13921 ttgacgatcc gtgcaactta ctttgcacag cagatgcaac accgccagat acgtctatat
    13981 gctggatttc gtgcagaact tcaattttat tccactctac tgaatcagaa tctgatttat
    14041 tatttatcta gctatatctt ttggatgctt atcggtaatc ctgaaattca ccattatact
    14101 tctgataaaa aaatactgat tatcagcgac ttaagtcttc gacatagtca atatattgaa
    14161 gaatatatca gtgatattct tgctgtgcat aaaattcact ctgagacaac tgctattact
    14221 gaagatcagc tgtcgaaata caatcttctc gagtatgatc tgatcgttac gaatcaaccc
    14281 attttgaatg cccatgttcc tcatatattg attgatgata gtgtgtcatt cgcaaatgaa
    14341 gaagaactca tccgcttatt cgagttatga ataatcaaaa aacagctatg tataaaccac
    14401 cctgataaaa atttcaggac aaatacacat agctgtttct ttttattgta ttccttcagc
    14461 aatccaacga gatttatagc gaaagtcttt taacaactct tcttttgtcc aattatttgc
    14521 aatgagatcc gtcaaagtgt tcattacgag ggtcatggaa aagtatagga aactaactgc
    14581 ttcttccgac gattgaatgg agaaatgatt gattcttaat aattccagcc atttttcgtt
    14641 cattggagaa tcagccgata gttctctacg tttggttgtc tgctgatcat ttcgctgggt
    14701 taacaagtat aacctttgcc aatagatact ttcgtgatct aagtcgcagc accactctaa
    14761 atatttttcg atccccaaaa agaaatgttg tttgttttcg ctaatgaatt ttaagatatc
    14821 ttgatggact atgtgagaac tgcttttgat cgtatacaaa taagcatctt ccaaatcttg
    14881 aaaatattta taaaaagcac cgcgcgacat ccccatcttg tcaacgattt ctgagacttt
    14941 gacttggctg atgtgctgtg agcaaaatat ctccagtaat aggtcatcga ttctctgttt
    15001 ctttgcgttc gataaattga aaaaagtttc ttttggcatc cacatctctc ctttatctct
    15061 gattatacgt ggatagccct tcattgtcat gaaggattca ttccttcttt ttctcttctt
    15121 ctgtcaagta cccatcttcc attcgataaa cgcgatcact ccattgaatc atacggacat
    15181 catgagtaac cataatagtc gctttttgtt tctcatgagc ttctttaact agtaatttga
    15241 cgacttcata tgcatgatct gtatccaaac tagctgttgg ttcgtctgct aagatcaagc
    15301 ttggctcatt atataaagcg cgagcaattg ccactctctg tctttctcca ccagaaaggt
    15361 ctttaggata actattttta agtgactgga tatctaaaga ttgcagcaag gaatcgattt
    15421 ttgttgaatc ttcttttttt gaagtaactt ggtcaactag ttcaaattgt tcctttaccg
    15481 ttaaaaaagg aatcaaatta gagctttgga gaataaatcc tatttcttta aagcgtaaat
    15541 ctgctcgttt tttttctgga agctcagtta gtgagtgacc gttgatcact atttctccgg
    15601 aagtcggtgt ttgcaatcct cctgaaatgg taaggaaagt acttttacct gaaccagaag
    15661 gaccaatgat gctgacgaat tctccttcct ttacataaaa atcaattgct ttcaaagctg
    15721 agacctttgt atgaccagaa ccaaatgttt tggtaacttt tttcatcgtt aatatttttt
    15781 tcatttttat taacctccta ttgctgtaat tgggtctact ttggttatcg tacgtatcga
    15841 aaacaatccg cctagcattg cgacaatgat caaaatccca ctatatagca accattgtga
    15901 ccaaaaaaca gcaaaaggca tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa
    15961 aagagcaagc agaacgccta ccaccccaac aataaaggac tgtgctacta aagatttagc
    16021 gatgaagaaa tttttgatcc cttgtgcttt cattactcca aaaatagcag tcttctgaag
    16081 tgtcatcacg tacatgaaaa taccgaccac ggcagcaacc actaagaaca aaaagtatat
    16141 catcgcattt aaggtcatat tttgggcaga atagccagga atactttcaa tgaatgtccc
    16201 aatagttaat aactgtagac ccttcgatgc tttttctttc gaaattttag ctgaatcctt
    16261 tattacgatc ccattgatcg gctgttcttc ttcagaagca aacggctgtt gtccatactt
    16321 caaagcagtc cacgtatcta gggacgcata gatgacggga gaaactgtgt aataagtttc
    16381 aggaaagata ccgacaatcg tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc
    16441 aattttatag cctgaatcag ccagattttg agagatgatg atttcattct ttgttttaaa
    16501 ttcatgccct ttcgtgagtt ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt
    16561 aacattttct ttgttttttc ctttgattgc tccgctgtac aatccgattg gtgctttctc
    16621 tcctccttca atgtatttca gatctcctct tgttaattgt gaagctgcaa atacttgatt
    16681 tgcatcctcc gacaaaacaa tctcttgtgc gtcccaatca tcgattgctt ttttaaactc
    16741 ttctgaaagt ccattcgcta gtcctgacaa catgaatacg acatacgcaa tcaagaacat
    16801 aataccgacg atcaagccat atcgtaattt agaatagcga atttctttga tagctagaaa
    16861 catttgtttt tcctccctat gatagattga tagtgacaaa atgtcacttg ttgaatagtg
    16921 taactctaaa gtgacaattt gtcacttgtg ttgttttttt cttatcaata aattgtctaa
    16981 gacatctttc gataaaacct taacatgagt cataaaaaat ttatttaagc cttatttatg
    17041 ctttccatga taaactaaag tgaccaaatc aatgaggtgg gaaaatgatt aaactattag
    17101 tagtagaaga cgataaagta ttatccaata atataaaaga aatactcgct gcaatcggcg
    17161 agataacaca agtttatgat ggtagtgaag gactgttcga gttggaaagt ggagtttatg
    17221 atcttgcaat cttggatttg atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa
    17281 tacggaaaga aaaaatacag actccagtat tgattttgac tgcaaaagat agtttggaag
    17341 ataagattga aggatttgaa cgaggtgcag atgattatct aacaaaacct ttctatcgag
    17401 aagaactgat catgcgcgtg aaagcattat tgaaacgctc gctaggtctc tttgatgaaa
    17461 atgttctgaa gtataaggat atcagttgtg atttaaaaag caatgaagtc ttgtataaag
    17521 aacaagtttt gcccatacaa ggaaaagagt ttgaattgct cgtttatttt ttacaaaata
    17581 agggaatcat tttgacaaaa gagcagatat ttgaccggat ctggggattt gattctgata
    17641 cgacgattac agttattgaa gtttatatga gcaacttacg aaaacatctg cgaagtactg
    17701 gattggataa agaaatcaaa accttacgta atgtcggtta tatactgcaa agggtgtgat
    17761 gcaatgaatg gagatattac acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca
    17821 gcatttgctt ttatcttttt tcttttggga ttcattactt tacagatatt aaaccaatct
    17881 gcatacagag aaacagatct gtcattagag aaaataaatt ttgattctcg aacagtacaa
    17941 ttagagatac aaagatatca gcaaggcgat ccctttttaa acccgactca aacggaacaa
    18001 aacagaacaa acatggaaca gccattaatg gaaccatcaa atgaccgttt taacactcaa
    18061 gtgatcttgt ggtctaaaaa tggtgagata ttgaataaag cagctattgg tgggcgtttc
    18121 aaccaaatca aagaattaaa aatggatacg aaaaatctga ataccatcca aaatattgaa
    18181 ttaaatgaag acagtggctc atctgccctc tctttccgtt cgattacgaa aaaagcggag
    18241 acgaatcaag atggcgttgc ctacatccaa attttagaaa atacaaatca aatcaagaat
    18301 tcattagcga cttttcgcac gattatgatt ttgtgtatga ttgttttttg gattctctct
    18361 attggtatca gttattactt gtctagtcta agcatgcgcc ctattattct ttcttggaga
    18421 agacaaaaag aatttgttga gaatgcttct catgaattgc gcacaccgtt aacgattatt
    18481 caaaatagtt tggaacatct ttttaccaag cctaatcata ctattattga agaatccgaa
    18541 agcatcgctc aagcattgtc agagacgagg cgcctcactg gattgacttc ggatttgctg
    18601 acaatcgctc ggaatgattc tgatcagcag ctgctttcaa agcaaatgat caatacacaa
    18661 gaatatatcg aacaacttgt taaaccattt caagagatgg ccatcataga tggaaaagag
    18721 tttattctcc ataacgatgc aacattagaa gtaccaattg atgaaaaaaa aatccatcaa
    18781 gttttggtta ttttgctgga taatgcatta aaatatacga atccagaaga taagatcatt
    18841 ttggattcat ctgttaacca taaagaatgg ctgattcacg taaagaatac tggcccttct
    18901 atcagtgaag aagatcaagc aaggattttt gatcgatttt atcgggaaga tcaatctcgg
    18961 gcgaaagaga ctggcggata cgggcttggc ttggcaattg caaaacaggt aatcgaacag
    19021 catcatggac agatttctgt atccaattat cttccacaag gagtcgattt taaagtccaa
    19081 ttgccgttga agcctaaaaa ataatgctta tccctaaaaa agggctgtga caaaaatctt
    19141 gtcacagccc ttttttgttg cgaataaacg gtgttccaga agtagcttct tcggaaataa
    19201 gccgaaattt ctttaagctg accacttctg tcacaacctc tttagcttac gcttacgatt
    19261 ttttattgcg gtcttcaatg tgtacatcac cgcttgtgtc aatatccaat tcttctcggc
    19321 taacatcttc tgtcactttt tcaacatctt catgttcttc tttatgaatc ccaacttctt
    19381 cacgaataac tgtatgtttg cttacatcta cttgctcttc tttgattggg atagtaatcg
    19441 tgtcatcttc caatttttcg tctgttccct gtgaagattg atctgttact ggtttgcgtt
    19501 cgatgacgat ttcttcacgt ttgaccggta cttctaccgt ttcagtatcg ttgaccacat
    19561 gtttgtggat gtcgacttct ccagttgtca catcatgtgt atttacatct aatttttctt
    19621 cttttagttg aattgtatca tcatctagat ttggtttgcg atcgatgtct tcagtcttgc
    19681 ttgtacccat tgtatcagtc tcgccaggta cgtccatcgg atcagttcct ggtgtcatat
    19741 tcaatggatc cgttgtattt tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc
    19801 cagtgccagt ttctaatcca gtccccattg tgcttgctgg gctaacagta tcttgtgtat
    19861 ccattgtatc ttctgttgtt tctggctgac gatagccttt tactgtgatg acaaattttc
    19921 catcagaaat atctcctcta tattgatata aaggatcatt ttctggtgta gccgtctcag
    19981 aatcataggt atcaaaagaa aaagtatctt ttattttttc ccataaagaa cggtcttctt
    20041 caccatccat ccgattttca tctgtttcaa catctatacc agacaatccg tcaaatccta
    20101 gtctagatgc tgcttcatca gtagtataaa taacgatatc atctcgttca tacccttcgt
    20161 tacgcaaccg ttcaattact gataatgctt cttccctcgt actatagctt ccgatgaccg
    20221 ttaaattttt ctcttccatg taaattcctc ctagcaaata gatttatgat tggtatattg
    20281 ttacttataa atttattcta taggagacga ttgttttcta caaatattta gattggaatt
    20341 tgattaaaac aatttaattg aaatttgaaa acatttatgt taatgaaagt catattctaa
    20401 taaaatgtca tttgtttcta tatgaatttt tatgaaaaat ttcatataat ttccactcgt
    20461 tgtgatggtt taatttatcc aatccatcaa taagcaaaca acttatataa aaaaggatta
    20521 attctcaata taattgctcc taccatagaa taaatcttca tgtaaagtaa tgaagtacca
    20581 actatttcta atacttcttt aagaattatc ccaacaatat cgttagacaa accattattg
    20641 aataaatgga gagccaatcg attctgaaaa aatgttttct aatttttcgg aaataaaaga
    20701 aagagcctcc tgccattaga acgaaccaaa tccatttcat tgctcatgaa gtaaccacat
    20761 agctaaaaag aggataaatc aattaaaaga tcaaaagaat gactgcacta ataaaataac
    20821 taaaaatgta tcttcttact tcatatcttt tagctagtac ttagttaaaa atttgcttcc
    20881 taagatcaag acaaactgtc tatcttcatt tgatgaaatg cggtaaaata tagaaaatga
    20941 acactattgg agggaatttg taatggacag caaaactaga aaacaactgc aagaaaagtt
    21001 aaagcaacgt acacacttgc tcccttccgt ctttcaacag tttttagaag aaaatcgttc
    21061 ttccctttca ttaggcagtc gctatgaata cgcaaaagat tttcatctgt ttactgatta
    21121 tttgagaaaa gtattttctg aagaagtgga tgatcagctg attatccatt tagaaaaaca
    21181 agattatcag aatttcttag ctaaaatcta tcgacatact cgttcgttcc aaactacagc
    21241 gaaccaagaa acggagcaat tgtttgaaaa tagctattac gggatttcaa ggaagcaata
    21301 ttcattaaat cattttttac gatttttgta tcaaaaagga ctgattgaag aagagatttc
    21361 tcttttgggt acaagtttac cggaaacaac caaagcagct ccgcgatata tcgatgcaga
    21421 acttttcaaa gattttgagc atttgtttat cccgattgaa ggctttcaaa caagcagaga
    21481 agctagcttt gaagaaaaga atagaccgag gaaccttgca attactgcca ttcttctgta
    21541 ttgcggatta aagatccatg aagtagtaga acttaaacgt aaagatgtat ctttgaccaa
    21601 gcagattctt aatctgaata gaaaagaaaa cagactgaat aaagtaccaa taccaaaaga
    21661 agcgatgcca tttctggaga actattttaa gttaacagca caaaatacgg acgaaaattc
    21721 ttttgttttt cgttcctcac atgatcagca aatttctcct cggactattc gccaaatttt
    21781 gagcaaaatt acagtatata aaaacgtatc gccagaacta tgcagaaaaa catatcgtta
    21841 ttatgcagaa ctatatttag atgatgctga cgcggtaaat tatttatgtg gtaatcgata
    21901 cctctcatcc cattcttttc aagatatctg ggaaaagatg gttgattttt cttatcacga
    21961 attagcggca ttcgttcggg tttcttccat ataaaacaaa tgtatgatct ttttttcaca
    22021 ataaactatg ttattatata aaggattatg ttacgattat cttgaatttg tatattactt
    22081 aacaaaagat aatggagtgt gaatgatgga gaccataacg tattcccctt tgaacttatt
    22141 tacgaatttt caagaagctg cagctaactt ttccgagacc ccgattatct ttgatgagcc
    22201 actttcagct ttcccagaat taggacttga aacaacttac aaaaaatgct gcgaaagtgt
    22261 gttgaaacgg gcttatcaat tggctcatct tggagtgaaa tccggagaca aagttattat
    22321 ttataagagt tccgcttttg acacatacct tctagctgtt tctgtgtctt accttggtgc
    22381 agtgcctgtc atgacttctt atcatctacc aacaacaaca atggaagtat ttatcgatcg
    22441 tttagaagac cctttcattt tatttgatga tgagacaaaa aagcgagtga gagaaatttc
    22501 aaatggaaca aaatcaaaac agattcctat tctttattta atggaacaac ctgctttgtc
    22561 tgtttctcaa tcatttttag acaaaaatga aatttcttat atgacccata cttctggtac
    22621 aactggtatc cctaaactta tttgtcattc tgctcattct atgggatggc ggacgaaatg
    22681 gcaaaaaaca gttttcacaa aaatttcgga aaagaaatta atcgcttttc acatctctcc
    22741 agtgcattcc agattcaata ttggtatctc ttctttgatg tccatgggat ttcctatgat
    22801 gcctttagca aacgcacaaa gttcaaaagt tgttcatatg ttggaagctc accggccaat
    22861 tgcactagag acccatccga ataactttgt tcagtggcgt tttactgcta aggagcatcc
    22921 agaagctttt tcgggtatcc gatactatca ctccactttt gatgctataa acaaccagac
    22981 aatgaagaca tttttgcgaa cgtcgatggc acaggatgcg gtatttcttc aagtatatgg
    23041 ccaaagtgaa tgcggaccaa tgattcttaa ggctcataca ctagagagtt tgaaaacaag
    23101 tgatgcaaga gatatgggcg ttggactgga agacatgaca agcgctcgga ttacagattc
    23161 agttgggaat gtcctacctg aaaacacaga tggtcatata caattactgt ctaaaggacg
    23221 ggctttgact tattacaagg aagatgcacg gttccaggaa aatgtttatg gcgattggtg
    23281 ggatagcgga gattacggtt ttatggataa gcatggccac ctatttttga aagatcggca
    23341 agtggatttg atcgaaacga tcaatagtac attagctatt gaagactttt tattagattc
    23401 gttagatttt ttggcagaag tagtgattgt aagggataaa aatcattctc cacaaccgat
    23461 tattgcttta gctcctgata aagaaatgga ttggaatcga tggtgggagc aagtccatga
    23521 acttcctcgc ttgaacgtcc ctatcatccg tgattttgat gcaatcccgc gaacagctac
    23581 aatgaaagtg caacggcttc aaatcgagaa agaattaaaa tcacaaaagt cctaactatt
    23641 taaggaaaga aagatccatt ttggtctttc ttttcttttt gtttttttaa attggatgag
    23701 ataaaaatat gccaattggc tgtttttctt ttttgaaaat ttcgttatac tcttattcat
    23761 ggaggaaaat gttatgaaaa gaatcttgac aattggcggt tctgatccat ttgctggtgg
    23821 aggaatccaa acagatttaa aaacttttga gaattttggt ctattcggac taagcgcctt
    23881 gacttctata ggggcactag atattaatga gcgatttatg ctggaatcta tatctttgga
    23941 attgcttaaa cgacagttag tttcgattga taaaatgact tcattagatg gtatcaaaat
    24001 tgggttattg aattcaagtg aagcaatcat ggttgttcga gacttcataa aaacaaaaca
    24061 aaatatccca atcattttag accctgtttt ggcattcaaa gaaactgaag ctgtgatcaa
    24121 tcaattgtat atttcgcagc tcatcgaaga actttttcca cttgttacga tgattacgcc
    24181 taatttggca gaagctgctt tactttctgg tcaagctctt ccctcttcgt taacagaaat
    24241 gatccatctc gctaaagaac tcactgcttc aggaacaaaa aacattgtga tcaaaggcgg
    24301 cacaaggatg gctggagatg aagctgtgga tcttctctat tcaattgatc attatgaggt
    24361 attttctcat aaaaaaatcg acactacaac agtaaacggg gccggttgtg ccttttcttc
    24421 tgcaataaca gcgaatcttg tgttaggggc agattttacc aaagcagtag cagaaagtaa
    24481 aaaatttgtt gcccattgta tttctaatgg cgtgctgatg aacgatcata ccggaagtgt
    24541 ctggtctggc ggtattcaaa aaggaggaat gagaaatgaa aactaagcaa ttaacgatct
    24601 atgctatgct gattgcgttg acagttgccc tttcattaac aattttgatc cctgtaccgg
    24661 ctacgaatgg atttgtgaca ttatgcgagg ccgggatcta taccgctgct tttctttatg
    24721 gtccaacggg tggattagtt gttggtgctt ccagtggtct attgatcgat ttgatttcag
    24781 gatatccgca gtgggcagtt ttttcattag tgatccacgg gttacaagga tatgttgctg
    24841 gttctttcag caaatcttct actcgtatgt ggggattagg gcttttatta ggaagcttag
    24901 ttatgatcat cggctatggt atcgccggtt ggattttata tgattggccg gctggagtgg
    24961 cttctatacc aggaaacatc ttccaaaatc tgattggaat gggacttgcc tttcctataa
    25021 ctgcaagtct tcaacgtatt caagcacgga agcaatatta attgaaaaag aaggttttag
    25081 aaaatgaagt tgtcagaaaa agaattaaaa gaccaattga ctgaaatagt taatgacata
    25141 ttggctgagg cacacttgaa aaaaggtgat ctttttgttt taggatgcag cacaagcgaa
    25201 gtcgttggag gacatattgg aaaaaattcc agtgcggaag ttggtcaatg gatcatccgt
    25261 acgttaaaag agttgttgga tcctaaagaa attgcgctag ctgtccaagg ttgtgaacat
    25321 ttgaaccgag cattagtagt agaacgaaca gtggcagaag caaaaaattt cgaaattgtc
    25381 tctgttgttc ctgcactaca tgctggaggt gcttgttctg ttgctgcttt tgaccagttc
    25441 aatgatcctg tcgaagtgga acacgtagtt ggacaagccg gtatcgatat cggtgacaca
    25501 tcaatcggta tgcatatcaa gcatgtccaa gtacctgtca gacctcgttt gaaaaccctt
    25561 ggacaagctc acgtgacagc ccttcgctct cgtcctaaat atatcggtgg tcctagagcg
    25621 aattattgaa atgagtaatt gacagagcaa tctgaaaaac ttcctcgcaa agtccaacta
    25681 atgaacttta acgaggaagt tttacttgtt tgttttatat aaccgagctt cataaggtgc
    25741 tagtatgcct ttagcttcaa attctccgct agaggaattt gacaagagta atgtccagga
    25801 ctgatctgct tcttttggaa gttcatagtc agcaagtgat tctgttaagt ttaccataat
    25861 aagatattgt tcatcttcta aatatctttc atagacataa agttgtggat gttcatgaag
    25921 atattcttta taagaaccgt atatcagacc tttattggtt tgtctaatgc ggatcatgtt
    25981 cttatagaat tgcaggaccg actctggttc atattcttgt tgttctacat tgatcgtttt
    26041 ggtatttgga ttagtgatca gccaaggttc atgtgtagaa aaaccagcat attgttccga
    26101 agtccattgc atgggtgtcc tgctgttgtc tctcgttgtt tcacgaatga tatccaatgc
    26161 ttcttctctt gtcttacctt ccgcaagcag ccttttataa agtcttttcg aatctactgc
    26221 atcaacttga tcgatcgatt caaagggcat attcgtcatt ccgatttcct gtccttgata
    26281 aataaatggc gtaccttgca aaaagaaata catcatagcg attgcttttg cagacatagc
    26341 ccaaaaatcg ggacgagtat ctccaaacac tgaaacggca cgaggaacat catggttttc
    26401 catatataaa gcgttccatc cgataccatc caatgaagtt tgccaagcgc ttaatgcatg
    26461 tttcaattta aggacatcta attgcccttc tttttcttgc tgccagatat ggatatgatc
    26521 aaattcgaat atcatatcga aataaccgtc tttccctacc cattgtggtc cttcttctgc
    26581 agtgacacca cttgcttctc cgacagtcat gatatcgtac tcttcaaaaa catgtttcaa
    26641 atcggtcaaa tgttcttcga ttcctgagac attttgaaag ggtgagaaag ggttctctgt
    26701 ggcttttact gaatattcat ctttttttac atgagaaatc gcatctaaac gaaatccatc
    26761 tatcccttta tccaaccacc atcggatcat attgaatagt tcttctttca atttctcgct
    26821 ttcccaattt aagtctggtt gttcttttgc aaaaacatgc agataatact gtccagtttc
    26881 atgagaatac tcccaagcag aaccgccgaa aatcgatgtc caattattcg gttctttgcc
    26941 atctaccccg tcgacccaaa tgtaataatc gcgatacggg ctttctttag attttttcga
    27001 ttcttcaaac cattgatgct gatccgaact atggttgatg acaagatcca taatcacttt
    27061 gatccctaat tcatgtgctt cttttagcaa taaatcaaaa tcttccatcg ttccgaatct
    27121 ctcttctata tcttgatagt cacttacgtc ataaccatta tcgacaaatg gagattgata
    27181 gatcggattg atccagataa agccgatacc aagatctttc aaataattaa gtttttcacg
    27241 gatcccattg atgtctcctt gaccatcgcc attgctatct ttgaaactag caggatagat
    27301 ttgatagcct actgcttctt tccaccattt ttgttcttgt atcatatcgt ttcactcctt
    27361 taacggtccc tcttgattat aaccgattct attttagaag tatccttgtt accggtaact
    27421 agaaacgaaa agctcaacga atctgctaag tgcgttcgtt gagctttatt ttatcataga
    27481 tagcgggcaa tcagttgaat catttgcttg ggaaattcat tcaagtcagt aatatctaca
    27541 aaacgacttt caccgtagat cgacttgatt tcttctttgt cttggccaat agctgcggag
    27601 acaaatagga ttccttgacg ttcatattct ttcaaaacag actggatatc ttcttttgct
    27661 tttttgcctg tataatccgg caacgctttt ggctgaccgt cactaatatt aagcaacagt
    27721 ttggttgttg cggattgtct gtttaatttt tctgctgcta aacgaagtgc agcaccatca
    27781 cgattgtttt gccttggttt catagtgact aatttttcat ttaaccattg aaatccgtct
    27841 tcaaattctt tataagaaaa caaagaagtc ttttctcttg acgaacggtc tgctgtatct
    27901 ccgtaaatca acaatgggat atcgactttc ttagaaaatt cggcaatggc aatcgctgct
    27961 ttttttgccg cttcgatccg atcatcccgt atcattgacc cagattcatc gatccgtaca
    28021 gctaccgcta aagaaggttg ctcatgtggc gggttctttt tgtcaaaatt tctcaaatca
    28081 ccatatgcta ctttgcttgc attgaaccgc tgaccttcgt attttccttt ttggtacgtc
    28141 tctgtttgtt cattatccag taactctaaa atttgccggc taagtgattc tactattggc
    28201 attacttgca tgtgtagctc acgtgcttcc ggcgtttttc cttggaaagc tggtcggtga
    28261 acgatgagac cttctttttc atgaatcgtt tccttcatga tttgtcgcgc ttctttattc
    28321 agttgcttct tgatttcctt atcatgtgct tccatgtcag aagcagacat ttcttgagaa
    28381 tcatctcctg tttgttgttc tttttgattt tttccttcga tatcgctact gcgttctttt
    28441 tgcatttgag gaggaatctc gtttttttct attgtttctt cttgtttctc ttctccctca
    28501 tttggttctt cagcagacaa cgatttcttt tcatctaagc gcagaatttc ttcagcattt
    28561 tcagtgatgt ttcgttcatt tgcttcttcg gcgctaatgg ttctttcttt tgtatgctca
    28621 gattgaggat gaccccattt ttcttttctc aatatgtcct gaagttccag tagcaatcca
    28681 ctgtcagaca aagcttgttc aagaattgcc aattcatctg aatcagtgga taatttatac
    28741 aaaacttttg gataaatcga ctggagtaaa tcttgtcctt gcttataggt atttgcccaa
    28801 taaaaataag aacgcattcc tgctacacct ttgatggcgt tagcttttgc agtttcatcc
    28861 aacaaacgga taatctctgc catctttaag agaagttctt gtttttggat catagttttc
    28921 gccatcgcac gttctgccat gacttctaag gcaggaagat ccattttttc cgcatgttgc
    28981 atccgatctc ttaaagattc atttaatggg cgatttcctt ggtaatttcg atttgtagtg
    29041 atcacaatga cgcaatctgg atggcgacga atgattcctg tcggtaaatt caacatccca
    29101 tttggttcga gcgctgagtt aagtgccaca agaacagagg catctcggat gacagtcggc
    29161 tcttgaatct ctaataaata gcctttttca atcgctcgaa cgatttcaga agggtaatag
    29221 ctgtaatgga ttgcatctgg ataatcagct tcgatccttt gaactagctc tgctaatttt
    29281 tctttagcag aaaaaggatc aatgccgtaa tggttctgga tcagtgtcag tacagcttca
    29341 agtgtatctg tttggtaaat agcttctagt aattcttggt cttgttcctt attagaatct
    29401 actacaggta ataaggctcc gaaaacatct gatttgtcca tatctgcaaa acaggtcact
    29461 tttgtatagg gaagttgcag atcagcagat agtgctttgg ctaattgtgt cttccccgac
    29521 ccggcatctc cttccaaaag tacgttactg attttcattt caggatcttg ccaattccct
    29581 ttgatttcgg aggcgatccg ccattcttct gcacttgtcc gatggctttt cggtttcttc
    29641 cagatcattt gcttttctag ccgtgaaaag gttcggtttt ctgttagtga gaaattctcc
    29701 atgcttctct tccccttttg atcatctctg tctattaata tcctagctca tctaaaagac
    29761 gttttaaaat acggaatcgt tccccaatca gttctccgtc tttttgtagt gtatcgtaat
    29821 acaaaatgat gtcttcatcg atctttggat ttttggtatc tacttctacc gtcattcggt
    29881 tcccttgtaa tccaatgatg tctacgactg gattttctgg atcataaaat ttttcataac
    29941 gataagcatc ttggaagtat aaacttcctt gcgcagctaa gatcgctaaa tctaaaatac
    30001 gtgtgattgg taattcttcc gactgtcgtg accatttttc tccagtatgt cgccatacct
    30061 tgcctgagat atcgactttt ccacgatcat tccactgagc aaggcccagt gaaagccctt
    30121 ttgcatccat atttaatgca tttcttccat caactttatc atagtcttct acaacgatta
    30181 ctggtttatg ttttaaattt gttggtattt ccattatttt aactcctcat ccttttattt
    30241 ttagtaaata agtaaattag tagtttactc atttactaaa ctatatgcga cattatagag
    30301 gattgaagtg aggattgcaa agaaaaagag cttcattgat agaaataaaa acaccatctc
    30361 gttcttttac acgctcatta gtaacatgta gaaagtccaa gatgatgttg gaatattttg
    30421 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgta
//
LOCUS       DAXLPI010000020        30318 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXLPI010000020 DAXLPI010000000
VERSION     DAXLPI010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.020"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            66..1325
                     /gene="lysA"
                     /locus_tag="ACF3T5_000800"
     CDS             66..1325
                     /gene="lysA"
                     /locus_tag="ACF3T5_000800"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFD3631431.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKERSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1312..2112
                     /gene="dapF"
                     /locus_tag="ACF3T5_000801"
     CDS             1312..2112
                     /gene="dapF"
                     /locus_tag="ACF3T5_000801"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFD3631432.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2115..2996
                     /gene="dapA"
                     /locus_tag="ACF3T5_000802"
     CDS             2115..2996
                     /gene="dapA"
                     /locus_tag="ACF3T5_000802"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFD3631433.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2993..3709
                     /gene="dapB"
                     /locus_tag="ACF3T5_000803"
     CDS             2993..3709
                     /gene="dapB"
                     /locus_tag="ACF3T5_000803"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFD3631434.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3715..4410
                     /gene="dapD"
                     /locus_tag="ACF3T5_000804"
     CDS             3715..4410
                     /gene="dapD"
                     /locus_tag="ACF3T5_000804"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFD3631435.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAGTVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4426..5427
                     /locus_tag="ACF3T5_000805"
     CDS             4426..5427
                     /locus_tag="ACF3T5_000805"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFD3631436.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5424..6785
                     /locus_tag="ACF3T5_000806"
     CDS             5424..6785
                     /locus_tag="ACF3T5_000806"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFD3631437.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYSKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6745..7896
                     /locus_tag="ACF3T5_000807"
     CDS             6745..7896
                     /locus_tag="ACF3T5_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFD3631438.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFHYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSRSKEERVYNDEFIQSI"
     gene            7871..9040
                     /locus_tag="ACF3T5_000808"
     CDS             7871..9040
                     /locus_tag="ACF3T5_000808"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFD3631439.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPKHVKQAGIAAIEEDFSHYTGMRGLEELREAACMFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9216..10595
                     /locus_tag="ACF3T5_000809"
     CDS             9216..10595
                     /locus_tag="ACF3T5_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD3631440.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSVSDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10876..11673
                     /locus_tag="ACF3T5_000810"
                     /pseudo
     CDS             10876..11673
                     /locus_tag="ACF3T5_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11862..12530
                     /locus_tag="ACF3T5_000811"
     CDS             11862..12530
                     /locus_tag="ACF3T5_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFD3631441.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12730..13116)
                     /locus_tag="ACF3T5_000812"
     CDS             complement(12730..13116)
                     /locus_tag="ACF3T5_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631442.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13088..14386)
                     /locus_tag="ACF3T5_000813"
                     /pseudo
     CDS             complement(13088..14386)
                     /locus_tag="ACF3T5_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(14500..16362)
                     /gene="ltrA"
                     /locus_tag="ACF3T5_000814"
     CDS             complement(14500..16362)
                     /gene="ltrA"
                     /locus_tag="ACF3T5_000814"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFD3631443.1"
                     /translation="MENVSASHSIRHMEYYGLIDSFDSLYKESSENKIFSNLMEIVTS
                     PNNILLAFRNVKGNSGSTSPGVDKKNIDDLKAIPNIEFIKTVQTKFSEYKPQPVKRVD
                     IPKPNGKTRPLGIPTIWDRIIQQCLLQVLEPIMEAKFHDKNYGFRPNRSAHHAFAQAV
                     RMAQLSKLTFVVDIDIEGFFDNVNHSKLIKQFWTLGVRDKWLLGVIRAMLKAPIIHRD
                     GRIEHPKKGTPQGGVLSPLLANVVLNELDWWISSQWETHPTRKNYDCYHQTRKQWIKS
                     NKYRALRASKLKEIYIVRYADDFKIFCRKRSDADKIFLATKLWLKDRLKLDISAEKSK
                     VVNLKKQKSDFLGFTMKLVRKRKSFVIETHMCAKAMSAASNKLAKQIKVIQHSPTQVE
                     LQKQLSIYNSMVVGIHQYYKKATHISVDCPRINQRLMIIIKNRLDIKRKGPPPSDFIK
                     KNYARSKQLRWLNGVPMLPFGFIKHSHTSLPKIGTCKYTPEGRVLIHRYLTFPIDIIN
                     QLMNMSNPNESIEFRDNRVSKYSSQVGRCRVTKEVLELADIYCHRIVPKNLGGKDNYQ
                     NLVIVHRNIHSLIHEEDKTRASLLIQNMRVTSAQLEIINQFRKKAKNRELSLNL"
     gene            complement(17312..18499)
                     /locus_tag="ACF3T5_000815"
     CDS             complement(17312..18499)
                     /locus_tag="ACF3T5_000815"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFD3631444.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(18506..19537)
                     /locus_tag="ACF3T5_000816"
     CDS             complement(18506..19537)
                     /locus_tag="ACF3T5_000816"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFD3631445.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(19553..21007)
                     /locus_tag="ACF3T5_000817"
     CDS             complement(19553..21007)
                     /locus_tag="ACF3T5_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFD3631446.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            21182..22087
                     /locus_tag="ACF3T5_000818"
     CDS             21182..22087
                     /locus_tag="ACF3T5_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFD3631447.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            22178..22855
                     /locus_tag="ACF3T5_000819"
     CDS             22178..22855
                     /locus_tag="ACF3T5_000819"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFD3631448.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            22852..23022
                     /locus_tag="ACF3T5_000820"
     CDS             22852..23022
                     /locus_tag="ACF3T5_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631449.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            23136..24032
                     /locus_tag="ACF3T5_000821"
     CDS             23136..24032
                     /locus_tag="ACF3T5_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFD3631450.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(24087..25457)
                     /locus_tag="ACF3T5_000822"
     CDS             complement(24087..25457)
                     /locus_tag="ACF3T5_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD3631451.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            25695..26441
                     /locus_tag="ACF3T5_000823"
     CDS             25695..26441
                     /locus_tag="ACF3T5_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFD3631452.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            26438..27277
                     /locus_tag="ACF3T5_000824"
     CDS             26438..27277
                     /locus_tag="ACF3T5_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFD3631453.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            27278..28228
                     /locus_tag="ACF3T5_000825"
     CDS             27278..28228
                     /locus_tag="ACF3T5_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFD3631454.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            28369..29121
                     /locus_tag="ACF3T5_000826"
     CDS             28369..29121
                     /locus_tag="ACF3T5_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631455.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            29140..30291
                     /gene="queG"
                     /locus_tag="ACF3T5_000827"
     CDS             29140..30291
                     /gene="queG"
                     /locus_tag="ACF3T5_000827"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFD3631456.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9776 a         5592 c         6310 g         8640 t
ORIGIN      
        1 attcaaatct ttatcaaatg aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg
       61 aaaaaatgac agaattgata attggcggag tcacagcaag cacattggca gaaaagtata
      121 agacacctct gtatgtgtat gatgaagaaa agatgagaca aacgatgctg tcattcaagc
      181 gaggctttgt atcgaaacgt tttgatacaa aagtacttta cgcctctaaa gcattccaaa
      241 cgattgagat gctgcattta gttcgtgaat atggattcgg gctagatgtt gtcagtggtg
      301 gggaaatcta taccgctttg caagctgatt tccctagaga tactatctac ttccatggta
      361 acaacaagac gccagaggaa ctgatctatg cacttgaaaa tgatttgttg catattgttg
      421 ccgataattt gatggaagtt gaattattag cagaattaag ccaacgctat ttaaaaaaaa
      481 tctgtatcat gcttcggttg aatgtcggag tagaagcaca tactcatgaa tatatcgtca
      541 ctgcgcatat tgattcaaaa ttcggcatga gctatgaaag taaagagtgc caacaagccc
      601 ttcagttgat aaaagaaaga agctatctag agttagaagg atttcatgct catatcgggt
      661 cacaaatttt tgatgttact gcttggttag ctgaaatcga caaattgacg aattacttaa
      721 ccgattttga ccaacctctt tccttgaatc ttggcggagg gtttgggatt cgttatacac
      781 aaaatgatca gccaatccca gtcgaagagg cactaaaaaa actgattgaa tatacggaag
      841 aagctttgga aaaacgaaaa ctgaccattc gtcaattact gattgaaccg ggtagaagta
      901 ttgttggaga agctggtaca acactataca cgattggttt catcaaaaag acaccacata
      961 aaaaatatta ctttgtagat ggcgggatga cagataatat ccgtccagct ctgtatcagg
     1021 cagaatacga ttgtgattta gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga
     1081 ttgcaggaaa aatgtgtgaa tcaggcgatg tggtaattaa agaagcttat ttgccgaaag
     1141 cagaatcggg tgatctttta gctgtttatt ccacgggagc ttatggatac tcgatgagca
     1201 gcaactacaa tcgagcgaca cgaccagcag ttgtttttgt caataatggc caatcaagac
     1261 tgattgttcg cagacaagca ttttctgatc tactaagggg ggaagtttcc tatgatcctt
     1321 cataaatata atggctgcgg aaatgatttt atcttattag attacgcaga agacacagat
     1381 tatagtcaat tagccgcttc tttatgcaca aaagaaaaat atgatacaga cggattgatc
     1441 gccgtaaaaa tcgatccgtt ggaaatgatc tattacaaca aagatggcag ccgcgcgccg
     1501 atgtgcggaa atggcattcg ctgttttgct cgatacgtaa atgatcagaa catgatgaat
     1561 gaacaggaat tcaacgtaga gacattagca ggcacaagaa aaattacggt gttggatccc
     1621 tcaccttttt attgcagcgt agatatggga gtgccagatg attctccgga aaaaatcaaa
     1681 gctaatcaaa cttatccaat aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg
     1741 atcactagtt tatttttagg aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag
     1801 gaagtctgga gaaaaagagg tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc
     1861 aatgtcaact ttgtagaagt agtcaatgaa caagaattga tcgtccgtac ctatgaaaga
     1921 ggagtaggtt ggacacttgc ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag
     1981 gaaactggga aaatttcagc agatcaggta gttgttcact tggaacaagg agacttgatg
     2041 atctctggtg aggatgcgat acaaatggct ggcccagcag ttcatgaatg gacaagaata
     2101 ttggaggaat gaagatgata aaaggttcga tcgtggcgct gatcacgccg atgaatgaag
     2161 agggatcagt agattatgct ggtttggaaa aattgatcca attccattta gatgaacaaa
     2221 cagacggatt attagtttta ggtacaacag gagaatcttc tacgctcaca caatctgagg
     2281 aggagcagat ccttcaactg acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag
     2341 gagctggaac gaataatacg aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg
     2401 gggcagacgc acttttggtc atcacccctt actacaacaa aacaagtgat gcaggactag
     2461 ctgctcattt tacagcaatt gcagaagcct caccgattcc gcttattttg tataatgttc
     2521 cttctagaac aggcatgtcg attccaatcc atgtattagt aaatttagca gaacatccta
     2581 atatcattgg tttgaaagaa gcctcaggag atatggctta tgtgatggat gccgctcggt
     2641 tgattggaga agaattcttc ctttacagtg gaaatgatga tttgatccta ccagtaatga
     2701 gtgtaggagg aagcggtgta ataagtgtat gggcgaatat ccaacctaaa atagtccatg
     2761 aattagtaaa agatacgcag gacggaagat ggcagcaagc aaaagaaaag caattaaatg
     2821 cacttgaact gatccatgcc ttgttcagtg agaccaatcc tattcctgtc aaagcagcca
     2881 tgtcattatt agacttacct tccggacctc tacgcctgcc tttagtttct ctttcaaaag
     2941 agaaaaagaa acaattagct cagcttttgt taaaagaaag gacggttgaa aaatgaaaat
     3001 cggactgatt ggcagtggac agatgggaaa tagagtcgaa gaagttgcac aagaaagagg
     3061 agatacggtc cttcttttac ggacggcacc taaagaagca actacgcttg cttttacgga
     3121 actgccagaa ctgcttattg atttttctca tccagataat ttagagaaaa tcctttcgta
     3181 tagcttgtct tatcaacttc cgttagtcat tggcacgaca ggttatacac aatcacagtt
     3241 ccaagaaata aaagaacatg ccaaacatgt acctgtgatg tatagtgcta atttttcgtt
     3301 cggcatcatg gtgatgaatc aactaatcaa gcaagcagct gcaatgttgc aaggctggca
     3361 gatcgaactg attgaaaaac atcatagtcg taaaaaagat gcaccatctg gtactgcaaa
     3421 tatgctgctt caaacaatcc aagaagtgca aaagctgcag cccgtataca actggcaagg
     3481 gaaaaaaaga gaagaaaacc aaattggtgt acacagtatt cgtgcaggtt ctttacctgg
     3541 tgaacatgat gtattgtttg ctgctactga tgaaatattt acgatcaaac atgaatcttt
     3601 ttctaatcga atctttgctg caggcgcagt agaagcagcg gactggttga aagatcagtc
     3661 accaggttat tacaaattag aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa
     3721 gctcaagaaa tcatccgcta cattcaaacg gcatctaaaa agacgcctgt gaaagtctat
     3781 ctgaatgtaa ccgagccaat cgaatttaaa gaaagtaaag tttttggtga aggaagcagc
     3841 ttcgttgttt ttggtgacta tgagacgatt gctccggttt tagaagctga atcagaaaaa
     3901 atcattgaaa tcgaaatcga aactgctgca agatacagcg cagttccttt gctagatatt
     3961 aaaaaaatta atgctcgtat cgaaccaggt gctattatca gagatcaagt gtcaatcggc
     4021 aacaatgccg tgatcatgat gggagcgatt atcaatattg gtgcagtaat cggtgaaaac
     4081 acgatgattg atatgggtgc tgtcttaggt ggacgagcga cagtgggcaa aaactgccat
     4141 attggtgcag gagctgtctt agcaggtgtg atcgaaccag catcggccaa accggttatc
     4201 gtagaagacg gcgtactggt aggtgcgaat gcagtgattg ttgaaggtgt ccatattgga
     4261 aaagatgcag tcgtggcagc aggagcagtg gttttagaag atgtggcagc aggaactgtt
     4321 gtcggtggaa tacctgcacg tgtattaaaa ataagtgatg atcagacaaa agaaaataca
     4381 gcattgattg ctgcgttacg cgaattatag aggagatgga gaaaaatgcg cgaaatagat
     4441 acctgtattg tgggggcaac cggattagtc ggccaaacga tgctcaaagt gttagaagaa
     4501 tatgattttc cagtaggacg attgaaatta ttagcttcaa aacgttcagc cggtaaagtt
     4561 tgtgtattca agaaaaagaa acacactgta caggaattga cagaaacttc atttgaagga
     4621 tatgatttag cgctattttc tgcaggtgct tctgtatcca aagagtttgc gccaatcgca
     4681 cgaagtcagg gactatttgt catagataat tcctcagcat ggcgggaaga ttcggatatt
     4741 ccattaatcg ttccagaggt caatctggaa gactatacat tgaatcggct gattgcaaat
     4801 ccaaattgtt caaccattca agcggtactt ccgcttaaag ctttacaaga agcctttggt
     4861 atccagcgag tcaattattc gacctatcaa gcagtttctg gttccgggca aaaaggcatt
     4921 gaagatcttc atcgtacgca aaatgacgag gaaccagttt tgtatcctta taatattagc
     4981 caaacggtga ttccggaaat cgatcagcct ttggaaaatg ggtatacaaa agaagaacag
     5041 aaaatgataa atgagacccg taagattctg catcagcctg ctttgccagt atcagcaacc
     5101 tgcgtacgtg tgccaatcga aaatggacat ggagtctctg tggcagttca actgcaaaaa
     5161 gagtttacga cggaagaaat tagagagtgt ctcagccagt ttccagggat tcaattagaa
     5221 gatgatctag cagcacataa atatccgacg agcattcttg ctagaggaac agatatggtt
     5281 tatgttggga ggatcagaaa agacacttct ttagagaacg gattgttgtt ctatacgact
     5341 gccgataaca tacgcaaagg agccgctgcg aatgccgtac agattgctgc agctttaatg
     5401 cagaaaatag cggaggtggc aagatgagta atacgctgaa agtagcgaaa ttcggcggaa
     5461 catcgatgtc tcatgaagag caattcaaaa aagtccgtac aattgttctt tcagatccca
     5521 atcgccaagt gatcgttgtt tctgctttag gcaaacgtac gtcagatgat gacaaagtaa
     5581 cagacttact ttatttgatt tatgcttacc ttcagcatca tgttgattgg acaccccttt
     5641 ggaaaaggat ttgtgatcgt ttcattcaag taagagatgt tcttgccctg cagttaccta
     5701 ttgaagatga actagcacgc atacagaaac aactggaaga cgaaaagata actgaagatt
     5761 atttagtaag tagaggtgaa tatctgactg ctcaactgat ggctgagtat cttggttacc
     5821 agtttattga cgcaaaagac ctgattttct ttgactatga tgggaaaatc gatcaggaaa
     5881 aaacagcaca ggaaatcaca aaagcattta aaagatattc gaaaatggtt gttccagggt
     5941 tttacggggc gaaccctgca ggaaaagaaa aattattggg ccgtggggga agcgacatta
     6001 ccggcgcgat actggcgaag atgttgcaag cggaaagtta tgagaattgg acggatgttt
     6061 caggaatcat gatggctgat ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct
     6121 cttttagaga actgagagag atggcttata tgggggctaa cgttcttcat gaagcagctg
     6181 ttttcccagt tcaagaggct gatatcccca tccaaataaa aaataccaat gatcctttcg
     6241 ctcaagggac aaaaatatcg aatcacgaaa tagaaaagga aaatggactg acaggaatag
     6301 ctggacgcaa ggattttctt tccatcactt tatttaaacg acatatgtcc gatgaaatcg
     6361 ggtttatctg gaaagcaatg agcatttttg ctaaacatga gatatcaatc gaacatattc
     6421 cttcagggat cgacaatatc ggagtgatcg tctcagcaga agcgattgcc gataaattat
     6481 ttctcatcac gaaagaatta aaagaaacac ttggcgtgga agaaatcgtc gtgattgaag
     6541 acttagcttt gatctctgtc gttggcggac cacataaaga attgatcggt ttatctggaa
     6601 aagtgttatc gatattgaac aaattagaaa ttcgcacatc gatcttatca caaggagcac
     6661 aggagttgaa tctgatcatc ggtgtaccga ataaccaata tgaaacagta gtgaagggta
     6721 tctatgaagg gatggtgaat acggatgctg aaagaatatc gaatggcact ccatcaaatt
     6781 cctgaaatcg gatttcatga attcaagaca aaaaaatatt tatatgaaca agtaaaaaac
     6841 agcggggccg ttatccatga aatcggcgaa acaggactac tcctttattt tgataatcag
     6901 caagaagaaa cgattgcttt tcgaacagat atcgatgcac tgccagtcaa agaagaaacg
     6961 aatctctctt ttgcttctaa gcatgaaggg tatatgcatg catgtggtca tgacggacat
     7021 atggcgatgc ttttagggtt gactgacttt ataactaaac acattgagga tcttatttat
     7081 aatgtagtac tgatctatca gccatcagaa gaaatttccg gtggcgcaca aagcgttatt
     7141 cagtcaggat tgttggataa atatgaagta caagcaatat ttggctttca tctatggcct
     7201 ggactagcta agggagaagt tttctctcga ccaggacctt tgatggcaca aagcagtgaa
     7261 acagacatta tcattaaagg ccagtctgca catattgctt ctagtgaaaa aggaatcgat
     7321 agtctggagg cagctgtacg atttttgaag aatgtttatg attttgaccg gcaattaccg
     7381 gcagagtgga atcatctaat gaaatttggc caaatccaag gtggaacgat ccgaaatgtt
     7441 ttagctggag aagttattgt atctggaagt atccgttctt acagttcaga tatgcaaaat
     7501 tatttgaaga ggggattaga ccatttagca gaacagtttc actctacagc tactatttct
     7561 tttcactata atgacggcta tccagctgta atcaaccata gaggcttata tgcagcactg
     7621 ttagattcaa acaaactaca tgaattagat gctccagttc ttcaagccga agattttggc
     7681 atatatacgc aacaatatcc atgtgtattt ttctttttag gtgtcggtga tacccctgct
     7741 ttacatgatc cgacgttcga ttttgatatg gatgttttag aaaatggtgt agaatggtac
     7801 aaaacgattc tttttacaga aaatcttctt gtaaaaatcg atagcagatc aaaagaggag
     7861 cgagtatata atgatgaatt tatccaatcg atttagtccc aggctttcta gaatagaagt
     7921 atcaaaaata cgacaattcg atcagcaaat atcgtcgata ccagatgtga tcaaactcac
     7981 cctgggagaa ccggattttc caactcctaa acatgtcaaa caagccggaa tagcagcgat
     8041 tgaagaagac ttttctcact ataccggaat gagaggatta gaggaactgc gggaagcggc
     8101 ttgtatgttt cagcaacagc gttatggatt aacttatgat ccgcaaacgg aagttttgac
     8161 gacagttggt gcaacggaag cgattgcctc tgcgttgtta tctgttttgg aagaaggaga
     8221 caaggttctt attccagcgc ctgcttattc aggatatcag ccattagtgg aactagctgg
     8281 tgctgaattg atcccgatag atacttctga tacagggttt gtctgtcagc cggaacaatt
     8341 tgaacgagca tttgaacaat atggatcagc tgtcaaagca gtcattttaa actatcctaa
     8401 caatcctact gggacgacat tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa
     8461 aaaatatccg gtatttgtta taagtgatga agtatatgct gaattgactt atagtggaac
     8521 gcatatgtcc atagctactt atcttccaga acaaacaatc gtgataagcg ggttatccaa
     8581 gtcacatgcg atgactggtt ggcgagtggg ctttattttt gctcaaaaag cccttattga
     8641 cgaactaatt aaagtccatc aatatctggt gactgccgct acaacgatgt cgcaaaaagc
     8701 tgcagctgaa gcgctaataa atggggtgaa agatagcgag aatatgaaag agcaatatca
     8761 aacacgaaga gactatctgg tccagcaatt ggcgccttta ggatttgaag tgacccagcc
     8821 aaacggtgca ttttacttgt tttgcaaact cccagctgcc attcaaactg actcttggca
     8881 gttttgtgta gaattagcgg aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg
     8941 cccagaagga gaaggttata tccggatttc ttatgctagc agcatggaaa aattacatga
     9001 ggcctgcaaa cgaatcaaag actttttaac agaacagtga ttcatagatt ttatcaaaaa
     9061 aacaatcgtt aattttgcgg tatattctgc aaaattaacg attgtttttt aaatttagtt
     9121 caaatgaacg cgtttctttt gattcataac taatgtaatg ttaagtttaa attgtttaga
     9181 atgattttaa aagaatattc aaggaggttt catcaatgga aaaaatcaaa gttgtcattg
     9241 taggtgcatc tcatggaggg catcaatcaa tcttggaact attgacacga tacggtgaaa
     9301 atgtagatat cacgctattt gaagcaggag actatatttc cttcatgtca tgtggtatgg
     9361 agctgtattt agaagacaag gtaacagatg taaatgatgt acgtaatttc agaccagaaa
     9421 actttccgca atccaatgtt catattttaa ataatcacga agtaaaaacg atcgatgccg
     9481 ataaaaaaac agtaacggtt actagcgtat cagataacga aacaaaagaa tatccttatg
     9541 acaaattgat tttgagttct ggtgtcaaac caaacaatct gccagtacca ggagcagatc
     9601 tagaaaatgt ctacttgatg cgtggataca attgggcaac caaaatcaaa gaaaaattaa
     9661 atgatccggc tattaaaaaa atcgctgtcg taggtgcggg ttacattggg atcgaagcag
     9721 cagaagcaag ccgaaaagct gggaaagaag tcgtattgct tgatgtgatc gatagacctt
     9781 taggcactta tttagatgct gagatgacag atattttaga acaacaccta aatgaaaacg
     9841 gtattgaagt tttgacaaat gcaaaaatcg aagcttttac tggtaacgga aaagttgagg
     9901 caatcaaaac aagtgaaaaa gaaatcccag cagatctagt cattcaagct gccggtgtga
     9961 aggcaaatac agaatggtta aaagggatcg tcgacttaga tgaacgtggc tggatccaaa
    10021 caaatgaata cttgcaaacg aatcttccag atgtgtatgc agtaggagat gctacacttg
    10081 cttattctat cccagcacgc aaaaaaatgc cgatcgcttt agcgactgtt gcacgtagag
    10141 aagcacgtta tgtagtaaaa catctttttg aagaagtacc aaacaaaccg ttcggcggag
    10201 tcgtaggttc ctctgcatta agtgtctttg actatcattt tgcagcaagc ggattgaata
    10261 gctttacagc agcaaaagca ggagtagatg tacgagtgtc ttattatgaa gatacgttac
    10321 gaccaaaata tgtaccagaa gaacaaggaa atccaaaagt gtccatccaa ttattctcgg
    10381 atccaatgac ccatcaattg ctaggaggag cagtattatc tacctatgat gttacaggtc
    10441 aaggaaatgt attggcacta gcgatccaaa acaaactgac tttagaagaa ctagcagaag
    10501 ctgacttctt cttccagcca ggctttgacc gacaatggag catgttgaat ctagccgctc
    10561 aacaagcgtt aggagaagaa ccatttgtag aataagaaaa cgaaatattt gaaaagaggt
    10621 tgtgaaaaag ctgccttgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    10681 tttttaactt ggctggactt attaccgcag atgcaagctt ttgaacaccg tttattttca
    10741 aaaaagggac tgtgacacga tttgtgtacc gacccccaaa agttagacca aaaaatctaa
    10801 cttttggagg tcggtatttt ttatggttaa atatactttt gaacttaaat taaagatcgt
    10861 tcatgattat ttggatggca aaggcgattg gaccgtaaaa atccagatca agagattgaa
    10921 gcggaaatgc ttaaaattcg ggagaagcac aaggattatg gctgtttacg gatgaccaat
    10981 gaactaagaa accgcggttt ctctatcaat aaaaagaaag ttcagcgctt gatcaagaag
    11041 ctaggaataa aagtaacggc atacacgcgt aaatcacgtc gctacaattc ttatcgagga
    11101 aaagttggag cagtagccaa aaatcggatc catcgtcgtt tctacaccag tatctgccat
    11161 cagaaaatca cgacggatac aactgaattt aaataccatg aagtggatgc caaaggcatt
    11221 atccgtcaaa agaaattgta tctagatcct tttatggata tgttcaatag tgagatttta
    11281 tcctatcgta tctctgaaaa gcccaatgca ttagcggtaa tggaaggttt ggaggaagct
    11341 atccagacca ctaacgattg tccctatcgt cgtacgtttc actcagatca aggttgggcg
    11401 tatcaaatga aagcttatag aaacaaattg aaggagtaca aaatctttca aagcatgtct
    11461 cggaaaggga actgcttaga taattcactt atggagaatt tttttggctt attaaaacaa
    11521 gagatttttc acgggaaagt ttataactgt ttcgaggaat taaaatcagc catcgatagc
    11581 tatatctatt actacaataa tgaacggata aaacagaaat taaactggca aagtctcgtc
    11641 caatttcgta aaacaaccgt aacggttgtg taaaaatatc attttagagc ataaaaaaaa
    11701 ggagtggatt tctccactcc aaaaaagtct aacttttggg ggtcactaca gtgtcacagt
    11761 ccttttttta gtaaaaggat acatttcttc cttgtatatt ccatgcgtgt tcgtgagaaa
    11821 gcatatataa tttaattaac agatagttag ggggagaaaa aatgattttt tcaattacaa
    11881 tgaaactggt gttcgggttg atcggtttat tattagtggt acgtttgctt gggaagaaat
    11941 ccatgtcaga aatcacaccg tttgatttag tctatacgct tgttttaggt gggatcttag
    12001 aagaatcaac ctatgatgac aatgtccatg tagggcatgt cctatttgcc attgcgcttt
    12061 gggcagtaat gatttatggt atcgaacgta tcgtccaaaa aaatgaaaag gtcaatcgtt
    12121 gggtcaaagg cgagccatct gttctaatca aagacggcgt catcaacatg acagagttga
    12181 caaataacca tatcgagatg gagcagttgc gagcaatctt aagacagcaa gaatgctttt
    12241 ttcttgaaaa tgccaaacat gttatcctag agaatgccgg tcaaatgagt gtattgaaaa
    12301 aatcagatga agacaaagct ttatcgatcc tgcttgtaga tgaagggcaa attcagcata
    12361 aagtgcttca gtccaatcaa ttgactgaag catggcttat ggagaacttg aagaaagaag
    12421 gctatgcaga tgtaaaacag ctgatttatg ttgaatggtc agaagaaaaa gggctctaca
    12481 tactaaataa agaagacgtc atgcatgaat cttaccgaat agatggatag atagacagag
    12541 gttgtgaaat cagcgccttg gcttgttact caaaactaag cgactttttc acaacctctg
    12601 atatctaata tacgttgttc cagaagttgc ttcttcgaaa aataagccaa aattccacaa
    12661 aaatttaaga agcaattttc gaaaaatcct ttcttatttt ttgaagttga tcacttctgt
    12721 cacaacctct catgtcacaa accatttttt tgtttccttt aactcaatat accgtacgtc
    12781 ttcccacaat atcggctgat ggtcaatcat aaagtaatct tcataggctt caccaatgaa
    12841 gtgcccttcg acctcatcca agagtcttcc aaattcatct tttaaattga gctgaatgat
    12901 tactgcttta cgagataaaa aggcttctgt cagtatacta tcgatttctt gcaatgtcat
    12961 ttgttgttta ggaagaggct gtttgcgtgc ttcttcttca ttttttttga taccgttgac
    13021 tagttcttcc atcgcaaaag cagtccccca ttttaatcca aatggccgat ctcgatactc
    13081 gttatagtca atgaattttt ttgctgtccg tctcatcttt tccaccttct aatccactca
    13141 taccacctgc atgcccgcca acgagagaac tacgtgcgat ggcgcgtcct ccttgcaaga
    13201 gactatgcgc atggatgatt gattgaaagc cgtatttttt tctaatcgta tcgacaacaa
    13261 aatcaacttt cgcttcattt atctgctttt caggatcttc aaaaaggtta agctgtaaac
    13321 ctgtcgcgta tacgaggtcg gaacagttca cgcctacacg ccgaatatcc tggtagtggt
    13381 aatgctggtg gaaaatggtc aataaagcat ctgctagatc tctgctagca tttgtcgctt
    13441 cgattttcat ttgctggtga aacccggtct tgccatttgg atcaagatag cctaaagagt
    13501 atcctaccca taagctgact acttgtgttt ttgctgcaga tcgtctcaag cgagtgccga
    13561 cttggtcggc catttctttg ataacgatct cgatttcttc tcgtcttgta taatctctat
    13621 ctaggatttg actatttccg atactttttg attttggtgt atattcttgt cccaaaaaac
    13681 ttcgatcgat gccccaagca tgggcataaa gctgagtacc gatgacccct agcttgcttt
    13741 tcaagtgata ataatccgcg tgagctaaat cataaacaga atgtataccc atttgtttca
    13801 gacgttttgc tgttcgtttg ccgattcccc aaaagtcagt gatatctgag attttccata
    13861 acttttctgg tacatcttca taacgccatt cagcaaacat ttgctttccg tcttttgagc
    13921 cattgtctaa ggctagttta gcaagtagcg ggttatcgcc aattcctata gtcgtgtaga
    13981 ttccggtctg ctcaaacaca tctttatgga tcagtttagc catgtcgtac gcatttgttt
    14041 taccaaagag tgccatgcta tttgttacat caaggaagct ttcatcgacg gaatagacat
    14101 gatggttcgc ttcgtctgca tattttttat agatagaatt gatttctctg ttcttttcca
    14161 tataataggc catccttggg gcaacaatat ataaatcact tggatagggg aagggaagat
    14221 ctctggcacg gctgatattg gtgatgccgt aagctttttt tgctgcagga ctggaagcca
    14281 agattaatcc gctaccccgt gtttctgtcg aatcggacgg ataggacatg actactaatt
    14341 tagttgtaag gggatcaaga tccttttcaa cgcactctac ggaggatagc gataggtaat
    14401 cctttgatgc aatctccaga ttttcccccg ctccacaccg tacgtgcgac tttcaccgca
    14461 tacggcgttc catcaaagtt tactaacagt atcatacagt tatagattca gcgacaattc
    14521 acgattctta gcttttttgc gaaactggtt gattatctca agttgagcac ttgtaactct
    14581 catgttttgt atgagaagtg atgctcttgt tttatcctct tcatgaataa gtgagtgaat
    14641 gttccggtgg acaatgacca gattttgata attgtctttc ccacctagat ttttagggac
    14701 aatacggtgg cagtatatgt ctgctagctc taagacttct ttcgttactc ggcaacggcc
    14761 gacttgggaa gagtacttgg atacacgatt gtctctaaac tcaatgcttt cattaggatt
    14821 agacatgttc atgagttggt tgataatatc gattgggaaa gtaagatacc tgtgtatcag
    14881 cacacgacct tcgggcgtgt atttacaggt tcctatcttg ggcagggagg tgtggctatg
    14941 ttttatgaat ccaaaaggaa gcataggaac accattaagc cacctcaact gctttgaccg
    15001 agcgtaattt tttttaataa aatcactcgg tggaggaccc tttctcttaa tatctagtcg
    15061 atttttgatg ataatcatta ggcgttgatt tattcttggg cagtccacac tgatgtgggt
    15121 tgccttttta taatattgat ggatacctac taccattgag ttataaatac ttaactgttt
    15181 ttgaagttca acttgggtag gtgaatgttg aatcacctta atttgttttg ctaacttatt
    15241 agatgctgca ctcatggctt tggcgcacat gtgcgtttca atcacaaagc tttttctttt
    15301 gcgtaccagt ttcattgtga atccgagaaa atcagatttc tgtttcttta gattcactac
    15361 ctttgatttt tcagcactta tatcaagttt cagtcggtct tttaaccata atttagtggc
    15421 tagaaagatt ttgtctgcgt cacttcgttt tctacagaag attttgaagt catctgcgta
    15481 tctaacaata taaatctctt ttagtttcga agcacgcagt gctcgatatt tgttgctctt
    15541 aatccattgc tttcttgttt gatgataaca atcgtagttt ttcctagtcg ggtgtgtttc
    15601 ccattgagaa gatatccacc agtccagttc attcaaaaca acattggcaa gaagtggaga
    15661 gagaacaccg ccttgcgggg ttcctttctt tggatgttca atgcgtccat ccctatgaat
    15721 aataggtgct ttaagcattg ctcggataac acctagaagc catttatcac gtacccctaa
    15781 tgtccaaaac tgtttaataa gttttgaatg gtttacgtta tcaaagaatc cttctatgtc
    15841 tatgtctact acaaaagtta gttttgaaag ttgcgccatt cgtactgctt gagcgaatgc
    15901 atgatgtgcc gaccggttag gtcggaaacc atagttctta tcatgaaact ttgcttccat
    15961 gatcggttct aaaacttgta gtagacactg ttgtatgatt ctatcccata ttgttggtat
    16021 acctagtggt ctagttttcc catttggctt aggaatatct actcgtttaa ctggttgcgg
    16081 tttatattct gaaaattttg tttgaacagt ttttataaac tcgatattcg gtattgcttt
    16141 taaatcatca atattctttt tgtcgacgcc tggggaagta ctaccagaat ttcctttaac
    16201 attccgaaat gccaatagaa tgttattagg agaagtgaca atttccataa ggttactgaa
    16261 aattttgttc tcgctactct ctttgtaaag agaatcaaag ctatcaataa gaccataata
    16321 ttccatatgg cgaattgaat gtgatgctga tacgttttcc atagcaccgc ctcctttcgg
    16381 acgtcggcgt ttattttgtc atactcgatt ctatggattg tatcataact gttagtaaag
    16441 caaattgact tgtggctatc cctccacagc ttattatcac tgcttcatag gtactgtgcc
    16501 actactctca ctagtgtaaa tgagcttatt attcttcgtc tcaaccgttt caaaggaagt
    16561 tagtcctcca cgacactagc tttcaacgtt ccaatgattt tatccctgca tttactttta
    16621 ggcgctcact ctgaacctgt aactttccaa tgcctactac attgcacggt tgttcatatc
    16681 tacgattctt acttaaccgc aagctacacc ctttgtcaca gtaatatttg tgcgggtaag
    16741 atacatttct gtaccccaag cttatagatt cgtacattcg cgagttcgtc agtgcagctg
    16801 cacattctca ccatgagtaa tttctggact cccggcctat aggttacacc attcaccgct
    16861 gaacagcttt cgattgcata atgcaactgc ggtaactctc agccgacttt accgagcttt
    16921 tgacaattcc tattctaact taaggtattg ccaatcggag tatcagaggg agcgcttcag
    16981 gtcgttacac cgtcatttca ctcctcaaat cattagttct acacaagaaa atcttgtgtg
    17041 cctaaccttt tcagttagga acgtttcgca ccgtaaaaag atttgcagtc aatacataaa
    17101 atatctcttg aaggttcttt ttcataaaca aataccggat tttgattcgc ccacataaca
    17161 atcacctctt caacacaatt agaacacacg ttctctttcc gtgtcaataa aaaacagcaa
    17221 ttttcaaata cgagcaaaag gctcacattt ggaaattgct gagaggttat ttttatctat
    17281 ttgtttttag ctgagatcgt tgattttttt atcaaacatg aacagcttct ggatcgatct
    17341 ttgttgtccc atttccaatg tgtgcaacat aaaattccgg agcatagccg acaactttca
    17401 tgtattcatc atagacattg tttttgaagg caggtatatt ttcttctttg acaagagcaa
    17461 tcgcacagcc accaaatcca gctcctgtca ttcgtgcacc taagacacct tcttgttttt
    17521 gagcagcatc tactagtgta tctaattcga tcccagttac ttcataatca tagcgaagag
    17581 agtggtgaga atcatttaac agttgaccaa atgcttctaa gttcccagct tctaattcag
    17641 cttttgcctt caaagtccgc tgattttcag aaacggcatg gcgagctctg cgtatcaatg
    17701 tatcatcgcc gatttggtca atagttgatt caaattcttc ttcatctaag tctcctaaag
    17761 aagagatagg caaagttttt tgcaaacgtg ccaaggcttc ttcgcattcg ctacggcgtt
    17821 cattgtattt tgaatctgcc aattcccgcc gcttattcgt attcatgatg acgattgcat
    17881 aaccatctag tttcactgga accatttcgt atttcagcgt atttgtatct aataagattg
    17941 catgatctag ctcaccgaag ccgatggcaa actgatccat gatacctgaa ttgacaccga
    18001 tgaattcatt ttctactttt ttacctgttt ggactagttc caagcgatct gtttttaaat
    18061 caaacagatc ttctaataca acacctacta ataattctaa tgaagcgcta ctggaaagtc
    18121 ctgcaccatt agggattgtt ccttccacta ataactcaaa cccatgatcg attgagtgtc
    18181 cagcttcttt taatttaagg accattcctt ttacgtaatt tgcccagttt gcttttttat
    18241 catattctag ctcatctaaa gtaaaactaa tgacaccgat atcgtcaaag tttgtggaat
    18301 aaactaatat tttattatcg tctcgaggtg ctgctacacc ttgcgttcca taagtgatgg
    18361 aagcggggaa gacatggcct ccattgtaat ctgtgtgttc accaatcaaa ttgatgcgtc
    18421 ctggtgagaa gtattgtgta gtttttttag gtccgaatgt ttcggcaaat tttgtgttta
    18481 agcttctttt gatttccatg atgagttatt cctttcttgt tttgattttg aattctgtca
    18541 ctgtttcaaa tactgatccg gcttttaaat gaatcgaacc aaatgctgag aagcgctctg
    18601 cacctggagc tgtttgagtc tcaaatgtga tgccaccgtg atgtgccagt tttcggttgc
    18661 gcatttctgg tgtttctgtg ccgaaatttg cagtgaaaat aaccacacta gaagcatctg
    18721 tagcaatatc tacttggatt cttttatcgg ggctagtcaa gcgagcggct tctttaccca
    18781 agccggtttc ttttaagaaa aatggatgat cgattccgtc aacaagattt ttttgatcca
    18841 aatcactagc aaaaacggac gctaattttt taggtatttg aaaatcgaaa gcacttcctg
    18901 cagcattttc ttttacccct ataggaatac tatctgttcg caaaggagca taagcttcgc
    18961 tattgagcca aagctcatgt tgatcgattg actgagaagc atctcccgtc aagttgaagt
    19021 aaacatgatt tgtcggattg aataaagtat cttggtcact ggtgccgcga gaggtcactc
    19081 gccagatatt atcttttgtc aaagtatagc ggatttccac atccattgta cctggaaaac
    19141 catgttctcc gtcgggactt gtagtagtaa aaatgacaga agcttcattt tcgccattca
    19201 aaatgacata agaccatttt tttaattcaa atccaggagc accaccatgc agattatgtc
    19261 cagtttttgg atctgtggct aattggtaag tttttccatt gagagaaaag cggccatttt
    19321 cgattctccc tgcggtgcga ccgatagaag caccgatata cggatctttt tcgatatatt
    19381 cttcagcggt gtcaaatccc aaaactaatt ctcgatcatt tgattttaaa ctgacgattc
    19441 tagcacctaa atcagagata gaaagtgcta gcttgttttt gttctccaag gttatcaagt
    19501 gataaccttg gccaaaatct ttttctgtaa tcttcatttt caagatcctt tcttaatggg
    19561 aaggaactac tgcatcgtga agttcatctt ccattgtatc agctggagcc aatttgtttt
    19621 ccaatttctc aacgatttgt gcatgttttt tctctgtcaa tgtaatcttc caagcaaaaa
    19681 taatagcgga gaaaatcatc aaagcagcag gagcatagaa tgcataagct ttgaaagtcg
    19741 ttacgccatg agcagtgata tcagcagcag tagcattacc agtcatccca gaagcgatag
    19801 cgacaaaccc tacgatcccg ttagacaaag caccagcgat tttatctagt aaagggcgaa
    19861 ttgatagagt aacagcttcg ttacggatac catttttcca ttgaccatat tcaacagagt
    19921 ctgtgatcgt catcaaagcg gataagaaaa tcaattgttg agggaagtag aacaatacta
    19981 gcccgatgat aacgattggc aaacttgaac ctgcgatcgt aaagaaaaag taagaagaaa
    20041 tcatcattgc gatccctcca aggtaaacgt agcgacgaga gatgacgcgt gtaagcattg
    20101 ggaatagtgg aacagctact aaaccaagta aggcagcaac tagaccaacg atccaaaatt
    20161 cagttggttg gccaagtaca tatttgaaga agtagaataa tacggcagtt gttgctacat
    20221 tggcaatcgc ataagcgata taagcaaggg caagccacat caactgatca ttttgagcaa
    20281 ctgctttgaa cacatctttg atcccagctt tttcattttg ctgacggatc gcagtatcga
    20341 cttcttttgt gcccaaggca gtgataatcg cagtaccgcc tgaaataaca gcagcaatca
    20401 caccgaacca gaaccagcct gaagcgcctt caatatgttt atgagtaaca agataggtaa
    20461 agaaagtaac gatagggaca acgactagag tagtaccatt tgcacctaga gaagagccga
    20521 aacgagcaaa cgtagcaaat ttttcacgtt cctttgtttc cgtactcaaa gctggaatca
    20581 ttgaccaaaa agcgatatcc ttaaatgaat aaaaactatc caataagatg aaaacgattg
    20641 caaataatat gataaataaa gtcgtattat gagtagctaa gccaaagaaa ttggtaaaga
    20701 ttgctaccaa gagtacggaa ctaacaacgc cgccgataac taaccatggt ttgaatttac
    20761 cgtattttgt acgagtatta tcgatgatgc caccaatcaa aggatcaaaa ataatttcaa
    20821 ctaagcggat acctacaact aagcttgtaa ctgtagcaat catttttgca ttcgttgcct
    20881 tatctgttcc tgcgaacatc tggcttgtca cgaataccat aaaataagtg cttaatgtaa
    20941 catagaatgt gtcgtgtcca aaagcgccta aagcgtagga cacacgttgg atcattttgc
    21001 ttttcataat ctgtattcct ctcctgttca atatataata ctaagtaaac cggttacaac
    21061 gaataacaaa gatcttttaa tgacttaaac ttaacaagaa atcgcttcct ttttaatata
    21121 tttctgtgac ttttatttaa ctttttgttg cattatacta aaggagtaag gagggataga
    21181 cgtggagcaa tcgatttttt taaaaaagga acatcaacaa gtagaaaaag aattgtattt
    21241 tgatttttgt ggtttctcta aaacgatgcc gtttcattct tttggtcctg ctattcgcaa
    21301 tgattacatc ctgcatgtcg tgatggaagg caaaggatct tatcatgtga aaaatcaaca
    21361 attccaattg aaaaaaggtg atctgttctt gattcgtcca ggagactcga cgttttactt
    21421 atcagatgga gaagatccgt ggatgtattg ctggctttca tttggtggga gtgctgcaga
    21481 agaaatcatt cggcagtcgc ttttcagaga agaccaatac acgatggtct cttctgacat
    21541 acaaaaatat atcgacatta ttttagaatg catgaaagat acggaagaaa ccttggaaaa
    21601 tgaattacag ttaacagcat taacctaccg ttttttatca gtccttttaa aagatggcgg
    21661 aaaggtcagc ggaggtgtac aaaaggttca ttcctcttta gctgttgaaa cagtcaaata
    21721 catcgaggca cattatgctg aaaaattgtc tgttgaggaa atcgcccagc atttagcggt
    21781 caatcggagc catttgtcgc gagtattcaa aaatcatatg ggcaccagta tcaaggaata
    21841 tttgatcggg gtacggatca atcgaggggc gtttcttctt tctctgacgg atgaatccgt
    21901 ggaaagtatt gcctatcaag tagggttcaa tagtctagtc gtatttagcc gaatgttcaa
    21961 aaagagtacc ggagaaacgg ctacgaacta tcgtaaacgt atgaaaaacg aaaattttaa
    22021 gaatattgct ttggatacct tgaaaaaaca gttggaaaaa caaaccgcag tttcttggac
    22081 aacctgatta gtctggttaa aatgacaagc atcggcaaaa gaaacttgtg gcggttatgc
    22141 tacactgtaa agcgaagact aaaaaggagg aaaagggatg cgtacactta tcatcattag
    22201 ccatccaacg atcgaagaat ctttaaatca gcaatttttc aaagaagctt ctgctggttt
    22261 accagctact tggcatcatt tggaatcaag ttatccagac ggaaaaatag atatagagac
    22321 tgaactacag ctcttggccc agcatgatcg gatcattttc cagtttccgt tttactggta
    22381 tagttctcct gctcatttaa aaatttggca agataatgtc ttggaacgag cagcacatgt
    22441 attaaaaggg aaagaactag gaatcgtatt gacaacaggt gtggcggaaa aagaatacca
    22501 agctggagga aaagaagaat atacgatttc cgaatttttg aggccgtatc agcgaatcgc
    22561 aaataaattc catatgacct atcttccgcc attcgtttta gcacaattta tgtatttatc
    22621 ccaggaaaaa cgttgggaga agctaattgc ttatcagcag tatctctcat tggaaggcaa
    22681 accatcgctg actcagcgga ttgactggtt tattcaaaga gtacaggaaa atcagaaaat
    22741 gcaggaagaa gaatctgaaa aacaagcata tatcgtagaa gcactgactg aggcaaaaga
    22801 gcagatcgaa gacttgacct ttacactgca agaaatgaaa ggaacttcat tatgacaaat
    22861 gattggctga tccaacaatt agatgaatta agcgaaaaat cagaatatca agaccgtgcg
    22921 ctcttatatg aaataaagaa actgatggct gagatagacg aacgaacaat gcagctgcaa
    22981 ggagaaatcg acggacaatc ttggaatcat ctcaactggt gaaattttct tataaataac
    23041 aaagataaaa atgtgcacag cgacaattat ttgctacaat aagaatatga agcgtttgat
    23101 aacgctatta tacataaaaa ttggtggtga aaaaaatgta tgatgaaaat gagtggggag
    23161 aaaatgttcg gatgacccga agaagacagc aaaaacaggc agctcgtaag cggatgatca
    23221 tgattggggc agcagttgcc gtgtttttga tcatagcagg aggagttgcg ggtctatatt
    23281 ttcatggaaa aaattcgtca gaagcgaaaa gtacgaccat aacggaaaca acgacaaaga
    23341 aaacagagac gaaacaaaca agtgagtcat caactaaaac aaaagagaca caaacttctg
    23401 aagaaaaaac agcaaatggt ttgccagagc aacttcaagc aatcgtaaat gattattcga
    23461 aaaagatcga agaaaagacg cctgcattga ttgaagaata ccagacagaa attcagggaa
    23521 atcaagaggg aatagctggt ttatcagcag ttgctaacca aaaagcacgt gagcttcaag
    23581 caatctccga cgaagggatc agaaaattac gtgcagcgta tcaggcagca gaaaataaag
    23641 acggtgtaga cttagatact ttgatcaatc aattatcggc taattatacg aatcatgtcg
    23701 ctaagatttc cgatatttat ttgcagacta gcgcctctct tcaagcagag tcaacaagca
    23761 cacaagacac aacatcttct gattctgaga cagcagagtc ggtagaagct tctgaaatgc
    23821 aagctcgaac aacacaagac acaacagatt cctctgaaac taataccaac agcgcgactt
    23881 cacaggctgc gacaaccgta gttttggaag gagaaggtcc aaatcaaata gccgaacgaa
    23941 ctggcgtacc agtagaaacg atcttgtcgc tgaatgggat gagcatggag aattatttcc
    24001 tgactcccgg tgagacgcta aaattgaatt gataaaagca ataaaaaaga acgaagcttc
    24061 cactaaggtg aagcttcgtt ctttttttat tttccggttc ttttttcgcg tacaaagtaa
    24121 tggatcgtca tgtaaagcaa gaaaacgagt ccaataatca atgagattct cgtttcagga
    24181 ttgataaaca tgaaaatcaa tacgatagct aaaaaagcaa gtgtcacata attcgtataa
    24241 ggagatagtg gcattttaaa aggatgatca tgcattttat ctgcgtgctt ctttttgaag
    24301 cgaatctcgc tgatcaaaat cactacccaa ggaaccatac caggcaaaat acttgagctg
    24361 tatacgaaaa cgaaaatatt ttctgactgg ttgatgaaca ttggtagaag ataatttaag
    24421 atcagaccaa ttaagattcc acctgagatt gctacgacag gataaaaagg cgcaccgtga
    24481 cgagatagtt tcagaaattt tttagacatc tttccattta tggcaagtgt atagaccata
    24541 cgacttgcgc tgaaaatccc tgaattgcaa cctgataatg ccgctgtaat cacgacaaaa
    24601 ttaatcagtc ctgcagcaaa tgtaatgccg actttagaaa aagtttgtac aaaaggtgaa
    24661 ccaatctcac tcaattgatt ccacggatag atactcacaa tgataaaaat agcaccaata
    24721 taaaaaatca agattcgacc gatagtggac tggacagctt caatgatcgt ctttttagga
    24781 ttctctgctt caccagcagt cattccgatc agttcgattc cttgatagga agcaacaacg
    24841 atcgataatg caaagaaaaa tcctttgacg cctccagtga agaatccacc atttttccaa
    24901 agatttgaaa tccctactgc ctgaccatga ttaccaaatc caaataagat taccccaaga
    24961 cctgcaatga tcatcaaaat gatcgttatg actttgatga tcgaaaacca aaattctaat
    25021 tcgccaaacg ccttgacaga aataagattt gccagcataa gaaacaagac gacaatgatt
    25081 ccgggaataa ttgccggcaa atcaggccac caataattca tataagatcc tacagcaatc
    25141 acttcactta ttcctacgac aatatattga aaaatattac tccaagcggt caagtagcca
    25201 gccacaggat ggatgtactc gctcgcgtaa tttgcaaaag aaccagtcga aggatcaacg
    25261 taaagcattt cgcctaaagc acgcataatc aaatacaaaa agaatcctgc aatcgcatag
    25321 gctcccatca ctgagggccc tgtccaacgt atggttgagg aggcacccat aaacagaccg
    25381 acaccgatcg ttccacctag tgcgatcatt tggatatgac gggcactaag acttcgagtt
    25441 aacttttctt cttccataca aaaactcctt tcgttgttga gtcaacaaga aaaaataatc
    25501 aaacgtttca acacatgttt ttcattatta ggttagcctt attgaactct tgatgtgatt
    25561 ctacagaaaa ttttcataaa atgcgaggaa aattcgaaaa aaactttttc atcattctac
    25621 ttgaaaatgc tttccatttt agttaaacta tatttaaata aaaagttagg taaacctaaa
    25681 aaggagtgta agatttggaa cgtacggcca ttgtaattca agacctttca gccgcttatc
    25741 aagggaaaac tgttttgaaa actatcaact tgacgataaa tccacaaaaa attaccggta
    25801 ttatcggacc aaacggtgca ggaaaatcaa catttatgaa atctttgcta gagttgatac
    25861 cagctactac tggtaaagtc accttttctg gacagccggt aaactcagtc cgtaaaaaaa
    25921 tagcctacgt ggaacagcga agtgaattgg atctttcctt tccaattgat gtattaggcg
    25981 ttgtactttt aggaacatat ccatctttac gaattggaca aagacctggg aaaaaagaaa
    26041 aagaacgtgc aagacaagct ttgaaaaaag tagggatgga agaatatgcc aaaagacaga
    26101 tcagcgaact atcgggtgga cagcttcaga gagtttttat tgcaagagct ctagcccaag
    26161 gagcagaatg gatcttttta gatgaaccat tcgtagggat tgatgcgtta agtgaacgaa
    26221 agatctttga catcttgcag gaattgaaga attcaggaaa aacgattttg atcgtccatc
    26281 atgatcttca taaagtagaa gaatatttcg atgaggttat tcttttaaat aaacagctga
    26341 tcgcttccgg tccagtacaa gaagtcttta catcgaagaa ccttcaattg gcctatggtg
    26401 agatcatcag acatttagta aaaggaggag agaaaaaatg atccaatcct ttattgatgg
    26461 attaatgaat tatcaattcc tgcaatacgc tttggtcact tccatgcttg taggacttgc
    26521 ttctggagtc atcggttctt ttattatttt acgagggatg tctttgatgg gagacgctat
    26581 ctctcacgca gttcttccag gtgtagccat ttcttatatg tttggtttta gttacatctt
    26641 tggagccact gcttttggga tgctaacagc agcagtgatt ggtttcgtga ctcagcggag
    26701 ccgattgaaa aatgatacag cgattggaat tgtctttagc tccttttttg cattagggat
    26761 tatcttgatt tcatatgccc agagtgcgac agatttgtat catattttat ttgggaatgt
    26821 cttagctgtt agggaaagcg atttgttgct gacagcactc gttagtggaa tcgtcttgat
    26881 atttgtcttt ttcttttata aagaattgaa gatcacatca ttcgatccga caatggcaaa
    26941 agcttatggg ctgaacacat cgctgatcca ttatcttttg atgttctttt tgacattagt
    27001 ggctgtagtc agtttacaga cagtaggaac aatcttggtg attgccatgt tgatcacacc
    27061 agccgccacg gcttacttgc taacgaacca tttactgaaa atgatcatta cagctgcagg
    27121 aatcggtatg ctaagtgcag ttgtcggtgt gtttttcagt tatagttaca attggccatc
    27181 aggagctacg atcgtgttag catgtaccgc attttttatc cttgcttttt tattttctcc
    27241 aacaaaagga attttatttc agaaaggaag cagatagatg aaaacaaaaa aatcactctt
    27301 tttaatcttg gcagttagtt tcttggtatt agccggttgc ggaaagcagg cttctgacca
    27361 agcggatgaa ggaagtaaag aaaagttgtc agtcgtggct accaattcga tcttggcgga
    27421 catggcaaaa gaggtcggaa cagatcaaat agatatccac agtatcgtac cagtcggaac
    27481 agatccgcat gaatatgaag tactaccaga agacatcaaa aaggcaagcg atgcagatgt
    27541 tattttatac aacggtttga atcttgaaac aggtaacagc tggttcgata acttgatgga
    27601 aacggctaaa aaagaagaag gaaaagatta ttttgcagtt agcaaaaatg tagaacctct
    27661 ttatttaact agcggtgaag aacatacaaa agcagatccc catgcttggc tagacctatc
    27721 taacggaata aaatatgtgg aggaaatcgc acgtatattc tctgaaaaag atgcagaaaa
    27781 tgcgacactc tataaaaaaa atgcagaagc atatgtggaa aaactaaaag aattagatac
    27841 acaagcgaag gaaagttttg cttctatcga agagaacaaa aaattattag taacaagtga
    27901 aggggcattc aagtattttt cacgagcata tgatttgcca gcagcttata tatgggagat
    27961 caatacagaa agtcaaggta cgcctgatca aatgaaagcg atcattgatc agataagagc
    28021 aaaagaagta ccggttttat tcgtggaaac cagtgtagac tcaagaagca tggaacgggt
    28081 agcaaaagaa acgggtttga aaatctatga taaactgttc actgattcca tagcaaaaga
    28141 aggagaacaa ggagattctt actatcagat gatgaaatgg aatattgaaa cgatccatga
    28201 aggtttgtca caaacaaaag aaagctgaaa agtgcaaggt ctagcttgag gagcagacct
    28261 tgcatttttt ataagagata tgaaaaaaaa ttatgctgtt tttcgtcgta cagatacctt
    28321 ttttgaagaa attccgttat agttagagta aggaaggaag gtgtggctat gaattttaca
    28381 cgtaaagata aatatacgat taaagaagaa gtctttcgat tgatcagcca gcaggaaaat
    28441 accgacaagg atcaagacct tcaatttctt gaagcagaat tagctaaatt attcatagca
    28501 aatcaaaatg agacaacaga accagatgaa gagctgtaca taggcaaatg gttgtttgat
    28561 gaatttcagc ctttagatga aaataccggt atcggttatt ttgtgttcct ttctccagtc
    28621 ggcttcacac ctggcgtatt agttaaacaa aacgacgaaa taaccgtcaa agcatggcct
    28681 gcattatttt cagtcatcaa agcagaagga gtaatcaaaa agatccagtc ggcctattat
    28741 aaaagtcaat ttattgccaa cagcaaatta atgaatatgt tttataatcg tctagtagaa
    28801 attgctaggc ctgggaaaat cccgcaaaca ttcacgtaca cacagcaaaa aaatctctat
    28861 gaagaagtat tgtattcccg cgatctgtct gagttttcgc catgggaaga ggtaaggctc
    28921 catgaactgt taagtacaaa accagtttca ttgaattttt ttaatgcatt agataacaaa
    28981 agaaaattaa tttcaacaaa ttattgggaa gtatttgctg caagaaaaaa atggatggaa
    29041 gaaaagaaga ttgccgatca gcagatccag tttgtccagc tgaaaaaaga aaaactatat
    29101 tatgggttat acagccgtta acagcaagcg aggaagacca tgacattaaa agaagaaatc
    29161 atacaagaaa gcaaacgatt aggaattgac aaaatcgggt tcaccacggc ggaacctttt
    29221 gattcattaa aagaatcgtt ggaagaacaa aaagcagctg gccatacttc tggctttgaa
    29281 catccaaata tcgatgagcg tctttatcca gaaatgacat ttgatcagcc gaaatcgatc
    29341 atttccattg cacttgcgta tcctactagg atccatgaag aagtgcctag ggatgaaaaa
    29401 cgcgggcagt ttgctcgtgc ttcttggggg atcgattacc atgatatact cagagaccgt
    29461 ttgaaccgat tgatcgaatt catccaaaca agagcagaaa aatggcaaaa agaagaggaa
    29521 tggcgtctag ctccgcaagt agatactgga gaactgatcg atgtcgctgt agcacagcga
    29581 gctggtttag gatttattgg acgaaacgga ttgttgatca ctgaagagtt cggttcattt
    29641 gtctatcttg gtgagatcgt tactaatatt gtatttgagc ctgataaacc aggagaattt
    29701 ggttgcggtg actgtatgcg ctgcgtgact gcttgtccga caaaagcatt gcttggtgac
    29761 ggaagaatga atgcccagcg atgtttgtct tatcaaactc agacaaaagg gatgatgccg
    29821 gaagaatatc gtaaaaaaat gcgaaatgtc atttatggat gtgatatctg ccagttagtc
    29881 tgcccttaca atcaaggaaa agattttcat tttcatgaag agatggaacc aaaagtagac
    29941 gaaatatatc caaaattaaa gccaatgctt tcgatgtcta ataaagactt caaacagcaa
    30001 tttgggcatt tagctggttc atggcgtggg aagaaacctt tgcagagaaa cgcattgatt
    30061 gctttagcta atctaggtga tcgaacggca ttgcctgaga tttcagaatg tttaacagat
    30121 gtccgccctg tcatcagagg aacagcagct tgggctattg gacgtctagg aacaaaagaa
    30181 ccggaaaaat ggcttgatgt attagatgaa atattagcta aagaaacaga agaagtcgtt
    30241 ataaacgaat tggaacatgc ccagaaatta ttgcggagaa agttaaaata aaaaaatagt
    30301 taacattatt caaagttt
//
LOCUS       DAXLPI010000021        29878 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXLPI010000021 DAXLPI010000000
VERSION     DAXLPI010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.021"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..451)
                     /locus_tag="ACF3T5_000828"
     CDS             complement(<1..451)
                     /locus_tag="ACF3T5_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631457.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEK"
     gene            538..1236
                     /locus_tag="ACF3T5_000829"
     CDS             538..1236
                     /locus_tag="ACF3T5_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFD3631458.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1414..1755
                     /locus_tag="ACF3T5_000830"
     CDS             1414..1755
                     /locus_tag="ACF3T5_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631459.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1748..2419
                     /locus_tag="ACF3T5_000831"
     CDS             1748..2419
                     /locus_tag="ACF3T5_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFD3631460.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2425..3111
                     /locus_tag="ACF3T5_000832"
     CDS             2425..3111
                     /locus_tag="ACF3T5_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFD3631461.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            3114..3863
                     /locus_tag="ACF3T5_000833"
     CDS             3114..3863
                     /locus_tag="ACF3T5_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3631462.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            3875..4144
                     /locus_tag="ACF3T5_000834"
     CDS             3875..4144
                     /locus_tag="ACF3T5_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631463.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            4149..4313
                     /locus_tag="ACF3T5_000835"
     CDS             4149..4313
                     /locus_tag="ACF3T5_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631464.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            4306..4608
                     /locus_tag="ACF3T5_000836"
     CDS             4306..4608
                     /locus_tag="ACF3T5_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631465.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            4605..4766
                     /locus_tag="ACF3T5_000837"
     CDS             4605..4766
                     /locus_tag="ACF3T5_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFD3631466.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            4763..5068
                     /locus_tag="ACF3T5_000838"
     CDS             4763..5068
                     /locus_tag="ACF3T5_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631467.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            5068..5424
                     /locus_tag="ACF3T5_000839"
     CDS             5068..5424
                     /locus_tag="ACF3T5_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HFD3631468.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            5384..5629
                     /locus_tag="ACF3T5_000840"
     CDS             5384..5629
                     /locus_tag="ACF3T5_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631469.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            5626..6045
                     /locus_tag="ACF3T5_000841"
     CDS             5626..6045
                     /locus_tag="ACF3T5_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HFD3631470.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            6042..6599
                     /locus_tag="ACF3T5_000842"
     CDS             6042..6599
                     /locus_tag="ACF3T5_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HFD3631471.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            6596..6892
                     /locus_tag="ACF3T5_000843"
     CDS             6596..6892
                     /locus_tag="ACF3T5_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631472.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            6969..7382
                     /locus_tag="ACF3T5_000844"
     CDS             6969..7382
                     /locus_tag="ACF3T5_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFD3631473.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            7691..7843
                     /locus_tag="ACF3T5_000845"
     CDS             7691..7843
                     /locus_tag="ACF3T5_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631474.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(7840..8115)
                     /locus_tag="ACF3T5_000846"
     CDS             complement(7840..8115)
                     /locus_tag="ACF3T5_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631475.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            8611..8775
                     /locus_tag="ACF3T5_000847"
     CDS             8611..8775
                     /locus_tag="ACF3T5_000847"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631476.1"
                     /translation="MPATLNAYDGYDYRKKIVKKLYGATYGNVVSVLSVHHQASVTVA
                     EVEDAAVNGE"
     gene            8971..9138
                     /locus_tag="ACF3T5_000848"
     CDS             8971..9138
                     /locus_tag="ACF3T5_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFD3631477.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            9164..9508
                     /locus_tag="ACF3T5_000849"
     CDS             9164..9508
                     /locus_tag="ACF3T5_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFD3631478.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9513..9794
                     /locus_tag="ACF3T5_000850"
     CDS             9513..9794
                     /locus_tag="ACF3T5_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631479.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            9897..10211
                     /locus_tag="ACF3T5_000851"
     CDS             9897..10211
                     /locus_tag="ACF3T5_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFD3631480.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10189..11883
                     /locus_tag="ACF3T5_000852"
     CDS             10189..11883
                     /locus_tag="ACF3T5_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFD3631481.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            11903..13081
                     /locus_tag="ACF3T5_000853"
     CDS             11903..13081
                     /locus_tag="ACF3T5_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFD3631482.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            13044..13730
                     /locus_tag="ACF3T5_000854"
     CDS             13044..13730
                     /locus_tag="ACF3T5_000854"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFD3631483.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            13730..14890
                     /locus_tag="ACF3T5_000855"
     CDS             13730..14890
                     /locus_tag="ACF3T5_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFD3631484.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            14900..15775
                     /locus_tag="ACF3T5_000856"
     CDS             14900..15775
                     /locus_tag="ACF3T5_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631485.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            15772..16083
                     /locus_tag="ACF3T5_000857"
     CDS             15772..16083
                     /locus_tag="ACF3T5_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631486.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            16073..16426
                     /locus_tag="ACF3T5_000858"
     CDS             16073..16426
                     /locus_tag="ACF3T5_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFD3631487.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16416..16817
                     /locus_tag="ACF3T5_000859"
     CDS             16416..16817
                     /locus_tag="ACF3T5_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631488.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            16810..17214
                     /locus_tag="ACF3T5_000860"
     CDS             16810..17214
                     /locus_tag="ACF3T5_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631489.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17226..17834
                     /locus_tag="ACF3T5_000861"
     CDS             17226..17834
                     /locus_tag="ACF3T5_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFD3631490.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            17854..18216
                     /locus_tag="ACF3T5_000862"
     CDS             17854..18216
                     /locus_tag="ACF3T5_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631491.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18219..18401
                     /locus_tag="ACF3T5_000863"
     CDS             18219..18401
                     /locus_tag="ACF3T5_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631492.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18418..21849
                     /locus_tag="ACF3T5_000864"
     CDS             18418..21849
                     /locus_tag="ACF3T5_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFD3631493.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVKKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSVLQEIGDRILS
                     STSVSSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKIQNDNASAWR"
     gene            21900..22637
                     /locus_tag="ACF3T5_000865"
     CDS             21900..22637
                     /locus_tag="ACF3T5_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFD3631494.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            22647..24938
                     /locus_tag="ACF3T5_000866"
     CDS             22647..24938
                     /locus_tag="ACF3T5_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFD3631495.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            24962..27088
                     /locus_tag="ACF3T5_000867"
     CDS             24962..27088
                     /locus_tag="ACF3T5_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631496.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            27105..27254
                     /locus_tag="ACF3T5_000868"
     CDS             27105..27254
                     /locus_tag="ACF3T5_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631497.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            27251..27697
                     /locus_tag="ACF3T5_000869"
     CDS             27251..27697
                     /locus_tag="ACF3T5_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631498.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            27699..27836
                     /locus_tag="ACF3T5_000870"
     CDS             27699..27836
                     /locus_tag="ACF3T5_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFD3631499.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            27874..28167
                     /locus_tag="ACF3T5_000871"
     CDS             27874..28167
                     /locus_tag="ACF3T5_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631500.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            28164..28388
                     /locus_tag="ACF3T5_000872"
     CDS             28164..28388
                     /locus_tag="ACF3T5_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFD3631501.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            28385..29410
                     /locus_tag="ACF3T5_000873"
     CDS             28385..29410
                     /locus_tag="ACF3T5_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFD3631502.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            29600..29673
                     /locus_tag="ACF3T5_000874"
     tRNA            29600..29673
                     /locus_tag="ACF3T5_000874"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29634..29636,aa:Ile,seq:cat)
BASE COUNT        10619 a         4546 c         6299 g         8414 t
ORIGIN      
        1 atttctctat tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat
       61 ttagagtaaa tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta
      121 cttcaaatgc aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt
      181 cagagtatat tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag
      241 tagctgacca tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg
      301 gttctgttga atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag
      361 aagcattaag ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa
      421 acgtactcaa tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat
      481 tcaaaataaa caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg
      541 caagaattag taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa
      601 agcttcgaga aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg
      661 tccaattttg gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga
      721 cgagttttct tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag
      781 aaagctatct acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt
      841 cgcaagaata ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg
      901 aaagatgtta aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa
      961 gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa
     1021 agccgagctt atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa
     1081 cgtacctttt caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt
     1141 tcatttattg aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac
     1201 agacaacaga gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac
     1261 ggcaattgct aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat
     1321 tgacaagcaa aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca
     1381 caaagaaaac acatcataag gagattttag catatggaaa aagaactttc cactctagat
     1441 caatatttga ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa
     1501 atcagacgaa atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac
     1561 catgtaacta tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct
     1621 gaatatattt acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat
     1681 aacttgattg atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca
     1741 gaatttgatg agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt
     1801 aaataaaatt ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga
     1861 gattatgaaa aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag
     1921 ggttgtttct ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga
     1981 tgaaactggg acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccgaatt
     2041 atttcctgtt ttagattatc gaaaccaacc agttgttaaa ccaaatgcaa tgcaaatcaa
     2101 taactcattg aagcgatgct ttgtgaaagc attggctcta cacggactgg gattatatgt
     2161 atttcaaggg gaagatattc caacaccacc tagaatcgat acaaagaaat taaacatgct
     2221 agagacgatt ctagaagctt tcaatgagca gatgggtaaa gatatgacca aaaccttaat
     2281 cgaatatgtt aatgagcaga cagataaatt agggctctta gctgataacg ttgaaactat
     2341 tgaacagtta agctatgagc aatgtgcctt gatggagcga gcaatagcag ctaagagaaa
     2401 agaattagat aagaagtgat atgagtgttt aaaccattaa tcgattcata ttcagcggtt
     2461 ctgaaaaagt tcaaaggaaa agacataagc gcaaccatca atgaggaagt gaacattgat
     2521 cgactaaaga cgatgtatga cggctacgat ggtgatcgag tcattgaaat tcgttttatt
     2581 gatcctagac gtttcaccgt acagcaacga aacttcatct atgcgctgat aggcgatatt
     2641 tttatcgata caggcatgcc aacggacttc tggaaggaat tcttctactt tcgttttgaa
     2701 ggtgtcacag ggcgcaaaat aagcctgaaa gacgaatcga atacaactgt gagtgatgcc
     2761 aatgtcttag caaatatcat cttagatttc atctttgaac atcatattcc tttcaaagaa
     2821 ggctatgaga ttttacctgc gaatcaagaa tattacttct acaaatgcat cacaaaaaga
     2881 gtctgctgca tctgtggcaa aacaggagct gacatcgatc actttgacaa agcgctagga
     2941 agacgaaagc gcaaagaagt tgatcatgca gagtacacat ttgcagcact ctgcagaatc
     3001 catcacacag agaagcacaa aataggtgtg attaatttca aaaataaata tcaaatcaaa
     3061 gggatcaaat taaaccagaa aacaatcaaa aagttaaata ttggagggta aaaatgacag
     3121 aacatcgaag ttattatgcg attataccag ccaacgtaag gtacgacaaa agacttaaac
     3181 caaatactaa gttgttatac ggagagataa cggccttgtg taatgaaaga ggcttttgtt
     3241 gggcaggcaa tgagtacttt gcagatttat atggtgtgaa taaagagacc atatcgcgat
     3301 gggtaagtga tttgattaag tttggatact tgaatcggga aatcatttac aaagagggta
     3361 ccaatcaaat aatcaatagg tacctacgaa ttaatcaata ccctattgac gaaaaacgca
     3421 atacccctat tgacgaaaaa gtcaaagata ataatacatc tattaataat acatttaata
     3481 atacaaaaga atatataaga gagttaccgc cttcgaaaaa atcgaaggct aagcccgtcc
     3541 gtcataaata cggagagtat aaaaatgttc ttttgtcaga tgagcagatg gagaaactca
     3601 aaacagaatt ccctaatgat taccaagagc gaatcgaacg actgtcagag tattgtgaat
     3661 catctggtaa gacttataaa aactatttgg caactattcg aagttgggca aggaaagaaa
     3721 aaagtgaacc taagaacgca agcagtggat acaagcgcac aggaagacga gagaagcttc
     3781 ctgaatgggc aatcgaccaa gaagcctatc tcaagaaaaa agcgctagaa cgagctaata
     3841 gacaatcaaa agcaccattc taagaggtgg aaaaatgaag atcgattatc tagaactaat
     3901 taatgaaata gcgaattata aaaagggcga ggaattagac gtcctgagag acgtatatga
     3961 tcaactcgaa gaagctggaa ttgaaggaat taagaatgat cgttcgagtt ggagtaagct
     4021 cagatactat ttcgcacttt atatcgatac aacacaatta agaaatttag catataccaa
     4081 attactattt gttgattgtg ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt
     4141 gtaatcagat ggacctaaag acatttacag cacagattga actaatgcat caagaagctt
     4201 taagacaaag tgtatcgtac gaagacaagt ggctcaacac gttccatggc ggacgtgaga
     4261 gcgcacttga tcaagtactc aaattactga aaggagaatg tcgggatgga taagaaagcg
     4321 gcaatgcagc gaattatcga attgacttat tcagaagatt ggcaaaatga caaagaagct
     4381 gcttcagaag tgatgaggct tggaagagcg atgtgggcag acaagagcaa caagccaaga
     4441 ccacgaaaaa tcgcaatttg gcacggtgac aaacttctag tgatagggac agctgaacag
     4501 ttagcaagtc tcacaggctt gcacgagaaa atcgtgagga aaagagcaag gtgtggctac
     4561 acagacgcta agaagagaac gtttagatac gtggaggaat catcatgaca acagaagaag
     4621 tgattcaaat gcgtattcga agccttcagc gtgagattga cgatctggaa cgaacaaagg
     4681 cagtgatggt caatgaaacg gcgagaaagg caatcgattt gcacatagag aatttaagaa
     4741 gggaaatcca tcgattggag gaatgagcgt ggataagaaa gcagcaatga aaagaattgc
     4801 tgaattaacc aagtcagaat cttggcaaga agacaaagaa atagttgcag aagtccaaaa
     4861 gctcggtaaa tcaatgtgga ctgaaaagcc caaacggaga acgccgagaa aaattgcaat
     4921 ctggcatgat gaccgaattt tagtaacagg tactgctgaa cagttatctg aaattactgg
     4981 attaagcaaa aacattatct gggatagagc taggagctta tggattgatt caaaaggacg
     5041 acagtttagg tatgtggagg agagataatg gatctcatta cacaatacag tgacatcatc
     5101 ctcaagaaaa tcatgatgaa gattcagaaa gataaaaaat caaaagaacg agctgaatta
     5161 gttaagttag aaatggctga aacaggagca ggagtgcgaa gtagcaggca ttggaaagca
     5221 gcagcaaaca ttgaatttta ttacaacgaa attcaaaaag ggttcgatca gatgcgtgag
     5281 ctggatcggc aaacaaattg gagcaagaaa cttcatcaag atcgtttcaa atttgtagag
     5341 aagtataaag aaatattaga agagtatttg aggaggacag caaatgataa aaaaactcgt
     5401 tcaattcagc atggatttat atgatatcga atcaggagca acactatctg tggaatcgga
     5461 ccatctaatc ataaattttg gtggaaagcg ccagattatt ttgtgggtag ttgatgatgt
     5521 actgtttcca gaaattgttc atgatttcga agaatcaaaa gcggttgagt ttgaaatagt
     5581 gaaaaaagta atggaattga ttgaaaaata cgaggaggac agcgaatgat accgaagttt
     5641 agagcgtggg ataaacgaaa gaacgtaatg agagatgtag ccgtcttgca ttttactaaa
     5701 aacggcaaaa caaactttat tgaatattgg ataaatccta ccgaattgaa atcatatcac
     5761 gtgcgaaaca tcgacctcat gcaatccaca ggaatgaaag ataagaatgg tgtggatata
     5821 tttgaagggg atatagtatt agtcagcgtg cgaaatggct tcgattactt agataataaa
     5881 gtctgtattg tcaaaaattc aatagattat tccggattag tttgtgccac tgttgatgaa
     5941 gacttagagt atcaaatttt taacacagag ctgtttgaag aatacacgta cgaagtcacc
     6001 ggaaatatat acgagaatag cgagttattg gaggaacagc gatgaataaa caggaagcaa
     6061 tcaaaaagtt agaaagtatt aaagcgatag gaaatgatgc aatagctgct tgctataacg
     6121 agagtataaa ttcaggtatt acgttaatga aaaaaataga cgaaccgcag aaaccagtta
     6181 ttccacaact cgtggccggt tggcttgaga aatctacgga cccttttaca aaagctgaaa
     6241 aaatagcgta tttaatcaaa tctaaagatg gtgattcata ttatttctgt gattggtttg
     6301 tacgagatgg catagtgacg caagagcaag gagaagaatt actcgcttgg gcaacaagac
     6361 aatcatatga aacactattg agcctataca acggctacga ggttgagaaa gagccgttat
     6421 gggcaataaa gaatgccgat ggaaactatc ttactaaatg tgctttatgg ggaaaagatg
     6481 gagtaaatta tagttttgaa tgcaatccat ctcatcgatt gcttttcact gataaagcaa
     6541 cagcggatgc tgcagcattg ttggtgaatg gaacagtgga agaggtggta gaaagatgaa
     6601 actaaaagac ggattttacg ctagcagtca cggtatcggc ggtttaatgc tagatatgcc
     6661 gacaaagaac cctaaaacac gcaagaaatc aaaattcaaa gtcggtgaca tggtccgctg
     6721 tgaagcagaa gagttcgttt atccgttcag aggatacgtt aaaaagatac tgtcaaactc
     6781 agcaatcatt cgtattgaaa atacaatgaa gtgtgatcgt tcgactgcaa aacacaagca
     6841 ttatttagct gtagcgagat tagttgacat ggaagtaatc aagagcaaat aaaaaaagcc
     6901 ggatcgctcc gactgattca ataaatccaa cacatttatt atatcacata aaggagcggt
     6961 ttgacttgat gcaattgtta cgagaggtag atttcaaaca gacaagatgt aatgcgagag
     7021 atgtgctgaa gaactttcgg cgtttggagc ggatggcagg tcgctctttg atagatatta
     7081 agtcgccgat tataacggat atgccgaagg caccgaagca tggcaataag gcagaagacg
     7141 cgattattca gatgatggat atagaaacgg agagagatgc gattctagcg gctttgatgg
     7201 ctcttagtct gattagtcgt cagatactct actacagctt ctgtgtgcca gatagcttct
     7261 caaactacag aattagccgt gaagtgggtt attcagaaag aagtatacaa cggatgaagt
     7321 cggaagctct aatagagttt gcagaagcat ataaacacgg aaaaataatt gcttataaat
     7381 aatttggcgg ttttttggcg gaatgatggc ggtttttaga catttaccag tgttattatg
     7441 gtagtgtcga aagattagga aacaggatcg acaaaataaa cgaagggagg caatctccct
     7501 catcgtttta aacttcttct tagatagaca gcaactaatt atgaagaaaa ggatgtgaat
     7561 tcaactcctc ctaaattctt catcggttgc tgtttatttt gattagacag cagcactcac
     7621 aaaactaaac atgttcttgt cgtctttcaa ctgctgctgt ctattaattt atgtattgga
     7681 gggaagaaaa atgaaattat cagtagaagg cactccagaa gaaatacaag aattgctcca
     7741 agctattggt agtagcaagg agcaaaaggt aagtgtttct aattcagata ttgacggtat
     7801 ttgcaattgt ttaaacagag aaaaaatatc taatccttac taagagaaaa tatgttcgtt
     7861 aatgaatttc caattttcct ctgtagctct accttgataa tttttttgca gttcacagat
     7921 gaataaggaa tcgttattat caagtactgt atttaatttt tcacacattt gatttggatt
     7981 caaattagtt ttaactaacc aaaaagattt ttgtagcttt atatatgccc caaattcttt
     8041 tattatttca aatactttat catacttttg ccctgggtta ttaagatcat aactaatcat
     8101 gtagggttta tacatgttgt cttgcacatc cttgttttat atttcagcgg accactcgct
     8161 gataaataaa attatacgct tagtatttat tttcacaata ttaatttatc gtaataactt
     8221 ttgagtaaat agaacaaaaa acctgcacta gtttccgcta gtacaggcag tgactatatc
     8281 ggttgataat ctagcatata ttaaaaataa ttgcaagaaa agaaatttat agtggtttat
     8341 accaaattat caaagcaatg ttattttgtt gctgtctatt gttttttaat tattcacacg
     8401 ataactaaag gtgggtgaag agaaatgatt ccattaataa tttcaatttt tgcgctctgt
     8461 cttaatgtct atatgattgg atttaaaaat gggcaaaata aaaaatagta gcagccaaga
     8521 ataattttat agtgtcactg tggcggaaag ggtagacgct aagcatgtgt gctaggtcaa
     8581 tgcttcggca accatgcaag gttcgattcc ttgccagcga cattaaatgc ctatgacggt
     8641 tacgactacc gaaaaaagat cgttaagaag ctatacggtg ctacgtacgg caatgtagta
     8701 agtgtgctat ctgtacacca ccaagcttcg gtcactgtgg cggaagtaga agacgcagcg
     8761 gtaaatggcg agtagcctcg tgagagcctg gtaagttctc gtgagtggtg caatcccact
     8821 ccagcgactt taagcaaccg aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg
     8881 tacgcgtttt gtgcatcgtg caagttacta ttacatatta gatcactctt tgagtggtct
     8941 ttttattttt gcacaaagga ggaaacaaca atgtatagac cacaatactt agaacagaag
     9001 tatgaagtaa tcactgttca taaaggtaat ggcgaaaaag tatatgagta tagaagacca
     9061 ataaagagcg atacatataa acgaaaggaa aacaatgaag ttattccatt gtatggcaaa
     9121 agaatagcta agcattaaat aagattgcga aaggagacgg aacatgaccg aggaattcta
     9181 tagatggcta ttacagttga caagagaaga tcgtttggtt aagttctatc agtctcctaa
     9241 atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc aagagtgtag
     9301 aagactaggt aagtatcata gagtagagaa cgttcatcat ataaaggaag tcaaggatag
     9361 acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac atcataatga
     9421 agttcatggc agatatctta cagcattaga taaacaagag aagaagatag aaagctttgc
     9481 taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgataat ggcagagagt
     9541 atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt aaacgtttga
     9601 tatacgttcg ctatgtaggt attagggatc tgttatcaga taactgttgt agtaaataca
     9661 aagtgaatca agtaagagaa gcgttgaata aagataataa cgtcgaaaga ataaaaaatg
     9721 tttttggata tagtattgaa gagattaatt attacattga cttcgctgaa gctttcattc
     9781 cgatggtgag ataacccccc cttaaaataa atcgcaaatt ttttgggggt gatgaaacgg
     9841 agggggctgt caggaaaaga gattttttcg aactttatca tgaaaggagg gctaaaatgt
     9901 ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt acgccgctct ttaataagcc
     9961 aattggaaag tcagaaaaca aatattgagc cattcttaga taatgttgat cgttatatca
    10021 gtttatggga aacggcgata tcactggaag aagatatatc cgagaacggc attagactgg
    10081 agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa caaatgggat
    10141 tgatgttgga taaacttgcc attactcctg aattggtagg tgaagcaaat gaatcaattc
    10201 ctgagttata agcatattga aaattggttc aaagctatag aagaaggcac tatcaaggta
    10261 tgcaaagagc aattattgct aaaaaattat ctagaagaaa gagtctttac tagagaagat
    10321 atttacttcg ataagcagat ggtagaggat tcaatcaata taccagcaca atactttcca
    10381 ttcgaattaa ttccgtggga aaaatttcta caatgtttta tttatggtgt tcgatggaaa
    10441 aaagataaaa cactagtgtt caatagatat ctttcattaa tgggacgtgg taatggtaaa
    10501 actggttttg cttcttggaa caacttcttt ctactaaccg ctaaacacgg tattaaaaat
    10561 tatgatattg atatctatgc caataatgaa agccaagcaa agactagttt tgatgatgta
    10621 tttaaagtaa ttaaagatca tcctgattta gataaaaaag tatttaaagc tacgaaggaa
    10681 gttattcaaa atatcgctac aaacagcaaa cttcgttata acacggcaaa tgctagaaca
    10741 aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa tgaagattat
    10801 tcaatgataa atgtggctac ttctggtggt ggtaaaattc gagattatag agaattttat
    10861 gatacaacta atggtcatgt tcgtggtggt ccgcttgatg acattataga agaatcaaaa
    10921 atgattcttt ctggagaact tggaattgac aaggatggag cagaattttc tagtttgttt
    10981 ccatttattt gtcgcttgga taacgataat gaagttgatg atcccgacat gtgggaaaaa
    11041 gcttgtccaa ctattaatta caatgcagat ttaaaacgga aaatgtttca agaatactct
    11101 caaatgcaac gtaatgctgg tttaagactt acgttcatga ccaaacgaat gaacagacct
    11161 atggaagata cacgatttgc tgttgcttca tatgatgatg ttctgcatac gaaagaaaaa
    11221 gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg attttgctga tagacgagat
    11281 tttgccagcg ttgggttgct aggaaaatac gataaagatg tttattttac acaacatact
    11341 tttatccacg aatcagccct tcgattacaa aacatcaaac gagaggttat agatatttct
    11401 atagatcaag gaaaatcaca gatcgttcat ggaaaaaata tagaagctga ttatattgta
    11461 ggttggtttc ttgaaatgag taataaatat tatattaaaa aaatcgctat ggatatgtac
    11521 cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt ttactgtgga aattgttcga
    11581 agcggatctg ttacacatgg tatgttaaaa gatctggttg atgacctttt tattaatcaa
    11641 cgtttatttt ttggtgacga tgcgattatg cgttggtatt gcatgaatgt atatgaagag
    11701 catatttcta atggaaatat acgctatgaa aaaatagaac ctgaaactag aaaaacggat
    11761 ggcttttttt cattccttca tggtttgaat tttttagatg atatttatga ttctgctcct
    11821 gtaacagtca caaatagctc agtagaaaat acaggaactg gatttactcc tctagtattc
    11881 taacttgaaa ggaggtgaga aagtggggat ttttcaaaag gcggtaggat acttcacaaa
    11941 aaaagcaacg gttcctttag aagaatactt ttgtaaattg caagttgatt ttgtgtatcg
    12001 aaaatttgcg attgaaactt gtattgattt gattgcaaat gcgatgagta aagcggaatt
    12061 caagtcatat gaagatggaa aaaataaaaa gaatgatctt tactataggc tgaatgtagc
    12121 tcctaataag aaaaataatg caacagaatt tagaaaaaaa ctgatcagga gattaatatt
    12181 ctacaatgaa gtattgatcg tttctccgtc taataattct agcgaaatat ttattgcgga
    12241 tagttgggat gtcacagaat atgcattgaa agatgatgtg ttttctcaag tgcaaattaa
    12301 caacatagtc cttgatagag aatttctaga aagtgatgtt atctatataa aatacgcaga
    12361 tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg tatgggaaac tcatttctag
    12421 tgccatgaat gtttacaagc gctctaacgc tcgtagatac gtactgaaag ggaatttatt
    12481 ccgaccgcaa gacaatacaa cacaagatca aatcaataaa atgatgacat cacaatttaa
    12541 ggcttttatg gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg agtacacatt
    12601 agaagatttc agcggaaact tccaaagcaa ttcaagagat ataaaaaact taatagacga
    12661 catctttgag atgacagcag cagcgtttca cgttccgaaa aacctactaa agggagacat
    12721 gagtgggtta tcggatcaag tggacgcttt tttaatgttc gaaatcatac cgattgctga
    12781 acttattcag gatgcgttta acgctagtct ctatgaagta gaagaatact tgtcagggaa
    12841 ttttgtacgt gtggatacaa ctatgatcaa gattactagc ttcaaagatt tggttgacgc
    12901 tattgatgta ggcattagaa atggagtatt tacaatcaac gaaggaagag aacgcgttgg
    12961 aaatgatcgc tctgataagg cgatggcaga tgaaatattt ataactaaaa acaatcaaca
    13021 agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac atttctagca gtaaagaatg
    13081 aaggcgcagt accgcaaatt tttattcagg gatttattgg ttctagttgg ttctttgaag
    13141 ggaatactga caagggaatc aaaaatattt tggatagtct aggtgatcaa gaagaaattg
    13201 aagtagtaat taattcaaac ggtggagacg tatttcaagg gattgctatt gggaacttac
    13261 ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg cttagccgct agtgctgctt
    13321 caattatcgc aatggctggc gatactataa aaatctacaa caatgcacaa ttgatgattc
    13381 accgcgcttc cacatacgga gaaggaaatg tcgatgactt ccgtacgatt gctgaccaac
    13441 tggaatcaat tgataaatcg gtaaaggctt catataaaac acgattcaat ggcacagatg
    13501 aagcattgca agaacttctt gaaaaagaat cgtttatgga tgcagaaaca gctttgagtt
    13561 atggattggt cgatgaaatt atcgatgcag aaaatagctc aggtactgaa gctaaaaaag
    13621 aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa aaaattgctg
    13681 catttacagc agcattaaat aaaacatttg gacaaggaga tgtaaaataa tgacagttaa
    13741 aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg aaagctttta aagatggtaa
    13801 cgaagaatct tttagcgcag ctatggtaag cttatctaag gaaattcagg ataaaatttt
    13861 agaagaagca acagcaaaaa atcaagatca attagtatta atgaaccgtg gtcagcgtgt
    13921 attaactaca caagaaacaa aattctataa cgaagtagtg aaaaacgaag gttttgcagg
    13981 ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta tttgaagatt tagaacaatc
    14041 tcatccacta ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag aatggattgt
    14101 gtcacgtgga gtcaatccag catggtgggg taaactttgc gaagctgtta aaaaagtttt
    14161 agataatggc tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt atattcctgt
    14221 atgtaaggca atgcttgatt taggtccagt atggttagat cgttatgtcc gtactgtttt
    14281 agtagaatca ttgagaattg cattagaaca agcaattgtt gatggtactg gtaaagatat
    14341 gccagtcgga atgatgcgtg acatgagcaa acaaactagc ggagaatatg ctgaaaaaaa
    14401 agcagaacct attacagctt tagatgctgt aactatgggc ggtttgatgg cgcgactatc
    14461 aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc cttctgatgt
    14521 ggtcctaatt gtgaatccaa ctgattactg gtctaaagta ttcccagcta agactgtact
    14581 aactgctaat ggagaatacg tacaagtatt gccagtacca gtttcagatt tgcagtcaac
    14641 ggctgtgcca gaaggaaaag cagttattgg ggtagcctca gattacttca tgggtgtagg
    14701 atctacacta aaaattgaag cttcagatga ataccatttt gttgaagacg aacgcattta
    14761 tctagctaaa caatatgcaa acggtcaacc taaacgtaac gatagtttca ttgtattaga
    14821 tattagcgct ttgggaacta ctactacaac tacaaaacca acaaccacaa caactacaac
    14881 acaagcgtag gtgatcagaa tgaagtatat tctttgtcag ccggcaatca atcggtttaa
    14941 atgggagctt gaagtttgtt taactaatct gaagaaacta ggaatcaaag atatcgtatt
    15001 gcttttcagc agacacgatg atcagattcc tatttttttt gagaaggaat atggtgttga
    15061 agttcatgtg tacgatgatc tgcgggacga caaagagtat attccttcga ttaaaccata
    15121 tttatggtgg aaatatttag aagaagatca ttcgcgtgag gacgaccgat atttctatat
    15181 cgattcggat gtcattttca ataaaagaat taatttgcgc aaattgcctt ctaaagatga
    15241 tgtttggtat tgtagcgact gctgtagtta tctaagtctt gattatatta gaagctgtga
    15301 aaacggagaa aatattctaa aagatatggc aaacattgta aatgttacag tagaatcttt
    15361 ggaaactata aacactaatt caggaggcgc acagtgggtt attaaccgtc ctaaagcgaa
    15421 ttattggaaa aaggtttatc tggattctaa tcggctatat cgctacctta gagggcaaaa
    15481 aacaaatata caaatctgga cagccgagat gtgggcacag ctttggaaca tgatgtattt
    15541 caatattggt cctaaagttc acgaggaatt agacttttgt tttgctactg atccaataga
    15601 aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta acaacaaacg atgaagattt
    15661 atttttcaaa gggagatacg tgacttctac gccttttgat gaagatttat catttgtaaa
    15721 caagaaaaaa tgctcttacg catatgttaa agcaattaag gcggtggtta gatgacgcct
    15781 gaacaagtga ctgaagaatt gctaatagct gtgaaggata atatttacgt tacctggaac
    15841 gaagaagatg agtcaattaa aaagatgata gctaaaaatg ctgtttatct tcaaagtaaa
    15901 gtgagtacaa cactttcttt ttctcctgaa agcttagaat acggattgct aatcgaaaga
    15961 tgtagatacg actggaatcg tgctttagat gagtttgaac aaaatttcgc tagtgagtta
    16021 ttaggtttca ttcaacatta tgcgctacaa gaatatattg caggtgatgt gaatggcgaa
    16081 taatcgtaga ctcgaagaaa cattcaacga tggttggtta aagattttga cgcaaactac
    16141 caaaagaaat gaactaggaa aaaagattgg tgtagaagat acagaaatca cttctttaaa
    16201 atttagaaat ctttccatga gagatagtga tataacagct atggatgcga tgggatcgaa
    16261 attaactaag aaagtaaaga ctccatttca tccaatcgcc aagaaattta ataaagatca
    16321 atattttatc gtaatcgata gtatgcgtta caacgttatc tatgccgatt acgataattt
    16381 ttatatctat ttttatcttg aaagtgtggg tgaatatggt gattgataat tctaaagaaa
    16441 aagaacgttt aaataagcaa atttctgcta tcaaaacttc cttagaagag cattttggcc
    16501 tcaaactctt tcaagactcc gtaggcgagg atgagctacc tgatgatttt aattacttca
    16561 ttctcgaaac aggagaaata gaaatgatca ctgagccaaa atatagcgtg ggtcaaaatc
    16621 tatatctaac tttctattca gaaaatagag aagatttaac aggagactca ctagatatta
    16681 tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat catttaaaac
    16741 tagagaacca agatcgctat atcgatcaat tggtatttac gtttagacga ttattgaaga
    16801 gtgattgtca tggctaaaaa tagttgggag ctaaaaataa atggacatga tgaacttctt
    16861 gtgcggatgg aacgctattc aagcgagagc gaacgactga ttaacgaagc attgaaatca
    16921 aaaggttcgg ctattgcagt ggataggatt acagaaaaaa ttcctgtttc tgaagcagat
    16981 ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca atacattaat
    17041 ttgggtttca tcattagacc tacaagaaaa tttgagtatt taaaatatcc tgatttgggg
    17101 ataggtactt ctaaaagaaa tcagccggac gaatttatga gaagaggatt aggtcttgca
    17161 cttgatccaa ttacagaact tctgattcgt caattcgata aattaaataa atagggggaa
    17221 caacaatggc taaaacaaaa actgtagtaa caacgttcga taacgtaagt atcaaacgaa
    17281 ttgcttttaa ttttaagaac gcaggaaatg caatcgcaac gaattgtaac ggacaattag
    17341 atggcgaaac agaaatgcaa acggtggtta aaaaatgtgg agcgacagaa gtaaaatcaa
    17401 aatctaaacc aatcaatatg acggtaacaa ttactgcaca tgtaccgatg gaagtttatc
    17461 gacgtttcaa tgggttgaaa caagatggac gtattaaacc aggcatttac tcttacggtc
    17521 ctgattccgt aggcgaagat ttctcacttg ctgcagagat cgtggatgac ttcgaagaaa
    17581 atagaaagtt agttggtatg ttagcatgca cttcgaatac aggattaaca ttctctattg
    17641 aaaatggtgc ggatgaagta gctgcgttag aactagaaac aaaagttatg caagatgaat
    17701 ttggtaaatt ttatcatgaa gcaattgttg cagaacttga agaagactta acagatcaat
    17761 ggatgacgaa tctatctgct gatgtgatta aaaaaacttc agttgtgaca actacggcca
    17821 ctcaatcaca gtaaaaaaaa cggaggtagc gaaatgaacg aagattactc aaaaattgaa
    17881 ctaaacgatg gaacaatttt gaatttagaa cctaaactga atatcaagaa attattgatg
    17941 atcaatagag attttaacac agacgagttt gcaaaaatga ctgtgggaaa aggatccatg
    18001 gatatttctg ttattcaagg tgcaaaggct gtgtatattg cttaccgcca agcgaacatg
    18061 actgattata tttcattcga tgaatttatc gataaatggg attttgatat ggctactgcc
    18121 agctatattt atcaattgat gatgttcaaa caagcacgcg atgcttatca aaaagaattt
    18181 gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat tccaaagctc ttagttgaaa
    18241 cgtgggtcga tgtctattcg atgttgaccg acgttttttc tatgccttca gatttggttt
    18301 taagcgatat ctgtttagat gacattttgc aaatggctta caacaagagt gcttatgaag
    18361 gatggaaaaa ctatgcaata aaccaatccc agaaaaacta aagaaaggag gtaaaaaatg
    18421 gctaaaaaga gaacagaagc agaagtaact ttcatagcta acgatgacgg attgaaatct
    18481 acgttaaaag aaatcagtgc tgaattaact aaaaatagag cagaattaaa actagaacaa
    18541 gctcaattac aacagactgg ttctgaatca gacaagttag gaagtaaatt atcttcttta
    18601 gaaaagcagt atgaattaca aagtcaaaaa gttgaagtaa ctagccaacg tttagccaat
    18661 gcgaaaaaat attatggaga aaattccacc gaagttaaga aacttgaaag agaactgatt
    18721 aatcaacaaa cagcgcaaca acgtttgtca aacgaaattg ataaaacgag taatgcacta
    18781 gctcaagcaa aaggcgaaat acagacgtac gagtctacaa tgcaacagtt ggatagcgaa
    18841 caaaaaaatg ttcaagctag tgcttctctg attgaatccg aatacaaaaa atggcaagca
    18901 actgctggtc aatcagcttc tgaagccgag aaattagcga aagcccaaga atatgtttct
    18961 caacaatctg aaaatgcgga gaaaacgata gatatcctaa gacgacagtt agaagctaca
    19021 cagtctgaat ttggcgctac atccacagaa gcaatgcaga tggaagcgaa gcttaatgat
    19081 gctgaacgtg aatttgaaga gttaggacaa gctgctaaaa atgtagatac aactaacttg
    19141 gacgatatcg gaagcaaaat agatatgaat aatttaatgg aagcttctga cgttttaagc
    19201 gacattggcg ataagcttac agaattaggg aaacaagcag tggactctgc taacagtgta
    19261 ggtagttccc agagtaagat acaagctaat tttggtttgt ctaaacaaga ggctgaagaa
    19321 ttaacgaatg tagccagaga catttattat aaaggttttg gagaatcgtt agatcagtcc
    19381 acagatgcat tgattttggt aaagcgtaat ttaggagatt taaataatca agatttacaa
    19441 aatatcacgg aacaagctat ggtcctagaa aacaccatgg gcgctgatat ggatgaaacg
    19501 ttacgtggtg taaatggctt aatggtcaat ttcggcttga gtgctcaaga tgcaatggat
    19561 ttaatggttt cgggtactca aaacggttta gataaaacgc acgaattagg cgacaatatg
    19621 gcagaatata gccaattatg gagtcaaatg ggatattcag ctgatgaaac gttcggaatg
    19681 cttcaaaatg gtttagatgc gggtgcttat aaccttgata aagtcaatga cttagttaag
    19741 gaaatgggaa tatcgttaac agatggtcga tttgagcaaa acatggatat gtttagtgaa
    19801 agtactagaa aagcttttga agagtggaaa aatggcggag gaacacaaaa agacgttatt
    19861 aattccatga ttcaagattt tagcaatatg gatggtcaat acgaccaatt aaataaagct
    19921 tcgacaattt ggtctgcgct tggcgaagat aatgcgatga aagttgtcca atctttaact
    19981 gatgttaacc atacatttga tgatgttagt ggatctgcac aaaaaatgaa tgaagattct
    20041 actactccgt tgcaagagtt gaacgggaaa atagctgaat taaaggattc attagctcct
    20101 ataggcaaca caatcataga tgcactcgaa ccagtaattg attttctagg aaagatggct
    20161 gatgcgttta ataatcttcc acaaccagta caggatttca ttgttgctat aggtggtctt
    20221 acagcagcat ttggcatact gttaccggca atactagccg tgtcatattt atttggtccg
    20281 atgatgctta taattggagg aattatagct gttatagcag gcgttattgt ggcaattaag
    20341 aactggggtg caattactga ctggtttagt ggtttatgga aaaaatttac tgattggttg
    20401 ggtgatactt gggaaagtat aaaagacgga gcctcatcag tttgggatgg ggttaaagaa
    20461 acctggtctg gatttgtaga ttgggttcaa gatatttggc aaggagtttc tgattggttt
    20521 ggagagctat ggagcggatt agttgaagga gcttccaaca tctggcaagg agtccaagaa
    20581 acttggcaaa cattcgttga ttgggtttca aatatttgga acggagtcaa agaagtatgg
    20641 tcgattattt gggcagacat tgtaggaatt gttcaaatac catggacatt aataacgtca
    20701 ttgattcaag ctggtattaa tattatcgtg ggtatttttg atgtagctgg acagttattg
    20761 ggcgcagctt ggcaagctgt ttggacacct atttctgatt tccttaaaaa tacttgggat
    20821 actatgacac aatggataag tatcgcttgg aatgggattg taactacatt ccatactata
    20881 tttgatccag tagtggcatg gtggaatggt atatggacag ctattagtac tacggcttca
    20941 aatatttgga attcaattag tgcaacagct tctagtattt ggaacagtat caagaataca
    21001 atcactagct tggtacaagc agctgctaca gtaattcaaa atatttggtc aactgtatct
    21061 agttggttag gtggaatttg gaattcaatc agctctacag catcaaatat ctggaatagt
    21121 gtgactagta gtataagcaa tgctataaac gcagctaaaa gtgccattca aagtgtttgg
    21181 aatagtatat cttcgtggat tagcggaatt tggaacggta tcaaaaatac tgctttgaat
    21241 ctttggaatg gaattacaag cactattagc tctaaagtaa acgatggaaa aaatgcaatt
    21301 tcaagcggtt ggtccaatct aacaggtatt gtttccgaca tattcaataa tgttaaaagt
    21361 acaattgcta acatatggga aggcatcaaa aagactgtta gcgctccaat tgattggatt
    21421 agagacaaaa tcagtggcat ttttgataat ttgaatattt ctataccaca tattccgtta
    21481 ccggaattta tcatggaagg cagctttaac ccgctaaaag gtcaaatccc ccatttgcgt
    21541 gtaaaatggc atgctaaagg aggtatcttt actaaaccaa ctttactagg tggaatgaac
    21601 ggtgtcggtg aagcaggacc tgaagcagtt ttacctttga aaagatctgt tttgcaagaa
    21661 attggtgatc gtatcttgag tagcacatca gtttcatcta gggcacaaac gattcaacct
    21721 gtgaataact acgaattcaa tttcacaatt gatggtaacg cagatgaggt tactatgaag
    21781 caaacaactc aacaaatcat tgatagcatt acaaaaattc aaaatgataa tgcttcggca
    21841 tggcgttaaa caggagagta tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa
    21901 tgactgattg tatacattct ataatcgatg gatttcctga ttatttgcat aaattggctt
    21961 tagctgaaag accaaccata ccttctccaa aaagacagag agttgaaact tctgttttag
    22021 gtaggttagg tggcttagta caagattact cgtttgaaga tatgtcgttt acattgcact
    22081 ataactattt agaggatgtg gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc
    22141 gtcattggtt aaactatgca aagaaattag aattctctga cgatcccaac gtctattacg
    22201 ttatccagac tatcgatatt ggggatgcag aaaacgatat tgttgaatgg ggagagttcg
    22261 atgtaaatat tactgcgaaa ccattcgcaa gagttcaaga agatgtacct ataaccgtag
    22321 ataaaccaca gtcatttaac ttgctgaata atagtttaga agaaagtttt ccaaagatta
    22381 tcatcactcc ttcagctact tcatgccagt tcatcttaaa tgattatgtg tttagtttcg
    22441 aaggcttagt agcaggaact gatgtagtca ttgatagtga tttgatgctt tgctacgaag
    22501 agcaatcgga cggagatatt ttagatcggt ccaacaaaat gaagaccatg caatatccga
    22561 cattgcaagt ggatatcaat cattttaatt gcactggttt gagcaaaata caaatttatc
    22621 gtaacgggtt aaggtaggtg aaatagatga tcgataattt aataactatt tacgataaaa
    22681 atgacgcgaa taatttagct gaacatttat atgatacgca aggtttaggc gctttgtcag
    22741 actggttaac agctactgtt agcaataaac taaacggagc cgagatattt cagggtactt
    22801 attcaataaa cggaactaat gcagatttga ttgtagaagg acgtattatt cagtgttatg
    22861 tagatgaaaa tcgagcaaaa cagcgtctac ggatttatta tgcaaaaact tctgtaatag
    22921 gcaatacgat agaagtaaaa gctgaaccta ttttcaatga tataagaaaa tcggtgttga
    22981 ataaatatga cagcggaaca gaaaagatca ctgctactca ggcatggcaa aacgcaaaag
    23041 ttttagcgaa accagctatc ccttcgcagt tttctttctc gtcattagta gatacgcttg
    23101 ctaatgtgaa gatagaaaag gcgaattttt tagaattctt tggtggaaaa gagggatcta
    23161 ttctagatcg atttcatggt gagtttctaa aagataataa cacattacgt catgaaaaaa
    23221 gtctaggtac ggatcataaa atcaaagcga tttatactaa aaacttaact ggtcttgact
    23281 tagagattga tgctcaaagt gttttagttg gagtttatcc attcattagc agctcttcag
    23341 aaggagaaga cgagatcact ctaccagaag aagttatttt cacggattac gtggatgatt
    23401 atcctgctgg atatgtttct tttgttgatt ttaaagacaa agcgactgat gtagccacat
    23461 taagggaagc tgctaaagac tggttgaaaa caaacataga taaacaaaaa ccacaagtga
    23521 gtggttcgat tgaattagta ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc
    23581 tagaaaaagt ttcgatgggt gacggagtag atgtgtatca tccacagtta aaagtgaata
    23641 tgtcagcgag aattgtggaa tatacgttta atgttctaac caattcatac gataaattag
    23701 ttgtaggaaa cgtcaaaaca aacttcttag aaaacacaga gaataatgtc agcaatttga
    23761 ttaatgatgc cattgatcaa ttgaaaaacg gtggcgaaat cagtgattta ctcaatgata
    23821 ttgtagatca tcaaactgat atgattactg gtcaaaatgg tggttatgtt ttattagatc
    23881 ctaaagaagc gcctagtcgt attttgatta tggacacacc agataagaat accgcaagga
    23941 atgttttaca aatcaacaat gctggtattg gtttttctaa aactggcatt aatggaacat
    24001 atgaaacggc atggacgtta gatggcggat tcaatgcctc gtttattacg gctggtgaaa
    24061 tagtaggaat tactattaga ggtactacat taattagtga tggtgctgat tatagaacaa
    24121 gtattgctaa tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc
    24181 tagaagcacg tgattatgta agtgctgatg ccggtattgt atcatacacc atgaaaactg
    24241 gtggtggttt catgattaga aatccacagg gtaacttggt ttttagtacg tgggataatg
    24301 gtaataacag accgtttcta tcttttggtg cgcccaattt caggtatagc aatgctagtt
    24361 atgtaacttc tggcgacggt agttctttaa gcattaatgg tagtgcgggt aactcatggg
    24421 aatttaaggt agctggtagg actatgaaat ttactagtga tggtatgcta acgttaccag
    24481 gttgtttttt tggttcatgg gaagatggga aacttgctag gtttgaacaa tcaacggtac
    24541 aagtatataa agattttact gttagaggta ctaaaaactc aactgtacca acagaacatt
    24601 atggacaacg actattgaac gcttatgaaa ctccagaata ttatttcgct gattatgggg
    24661 aagccgttac aggtgacaat ggtaaagttc gtgttgatat tgaccccatg tttgctgaaa
    24721 cagtaaatct aagtcggtat atgacacatg tgacacctac agaactagtt ttgtgtgctg
    24781 ttactcatga agatattgac catttcatca ttgaaactag taagccaaac gtattagtta
    24841 gatggaattt agtggcacac cgtctagggt atgaagatat tagattaaaa gaggatacag
    24901 catatgatag cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag gaggtatata
    24961 aatggctagc agtttatata atttggcttt agatttcagc aaagaattaa actacaccaa
    25021 agctattatg gctcgtcaag gtgataaagg gattacggtg acggttaaac cgtttctaaa
    25081 tggcttgcag atggatacga gtggcggaac atttacttta aaaggaacaa caccatctaa
    25141 ccgttacgta gataatgttg caactagtgt aactagtgaa gaagtcacgt tttctcttga
    25201 tggcacattt atgagtgaag caggatatta taaacactgc tacgtagaat atagaaaaga
    25261 caatcaaatt ttaacaacgc aagatatcat ttttttctca ctaggagtgt ctgacatttc
    25321 gcaaggccaa gccgatgaat atgtttcgca attagaagag ttgattcgaa agtacaacga
    25381 aacttttgat gcttttatgg ctgaaatcaa aggtagagtg gatagcttaa atcaacagat
    25441 tactgattta actggtcaag ctaaaacgct acaagacaag ttagatgctc tgaaagaaga
    25501 aatttctaag ttaggtaact tacaagtgat gtacagtaac agcatcgact tcggggacta
    25561 tgattattcg gagaacccga atttaatgcc atatattaca gaaccttggg ttggaccttt
    25621 gttaggtaat ggacatacag ttaaagatag tgttaaaagg gttataactc atactaaaac
    25681 tagaacagct aattccggcg acatattatc gcttggctta ggtattccgt gtacagcgga
    25741 agctaataat aggtatctta taaccacttt acgaccatct acgacataca cattaagcgt
    25801 tactatgagt gtcggttcag attggactgg agagaccaat actatagggg ttaggcttag
    25861 atatttaaat gaacaaggtg gtatagaact gcccattaat gcattaatac cagcaaatgt
    25921 agaacgcgat aaaatggtca ctcatacatt tactggtata acaaaagata atgtaactag
    25981 cataacaaac tgttatgttg aaatattctc acttaatagt gaatataaag gaacggttag
    26041 tgtcagctat gacgttaagt taaaagcaca ctaccctaac ttactagatg gcccttactg
    26101 gttaggtaaa gttcctttag gcgagaatat tgctgaccct acagttgtct ttccacacaa
    26161 aactagtgaa tatatggttt atggtagacg caatacagaa aactatatcg ctgaccaaac
    26221 atataccata tcaatgaaag caactaaact aacagttcaa tcgtttgccg tgtatattgc
    26281 tgctggccgc gttaaagtag gagacatgaa gcctactgag gggttagcta atacatggga
    26341 gttaactttt actgtaacta agcaacatat agatagtggg gtaactaatt atctggaaat
    26401 ataccaatac ccgtcagcta caaaaggagc tgttcaaatt gagtggctca aactagaaaa
    26461 aggcaatact cgaaccccga atattagtga gtataaatat cgtggtactg gtatgcgtga
    26521 ttcaaacaat ccaaaagatt atgtttggga tctagcacca gaatatgtcg aagataattt
    26581 ggccacagat attaaaattt ctgaaattac tggtaaagca aacaattata ccgatgggaa
    26641 agtatcggag attaattcgc agttgactgc ttcaattaat gaagtagaca ccacagctaa
    26701 ggatgctcaa acaaaagcga atgctaatgc gactgctata gatgaattag acaataagat
    26761 cgatgaacgc attaatgata cagctactac cacattaaca gttacaaacg ggaataccgg
    26821 atcagcaaag ctttatcgtg aaggaaaaac agtttctata tattttgtgg ctttaaacgg
    26881 aaaaagcagt ggtggaaatg attcaacgat actaacaatt ccagaaggct atcggccacc
    26941 aattagtttt gagcaactgg ttggctcgat agaccgttct actttgaaca gtgctcagtt
    27001 atctattggt gcagatggag ccattaaatg gcgaagaaac tcaagttatg gatcggatta
    27061 tacctttgca attacttaca cgatttagaa aagcgtgaat cgatatgaag gcagcatata
    27121 gaccaattga accttacgga ttcgagcaaa tcattgtgaa tgatgaagaa catttaccgg
    27181 aagaatgcac agaagtcgaa ccaccgattc caaattggaa accgaaattc aattactggg
    27241 agggaaataa atgaaaaaca tttggaaata tggacgtact ggcggagagt acgcaggaaa
    27301 agtattggac gacatgcttg tatccgttcc ttacacagat cagccaccgc ttgaaggaat
    27361 tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg tttgatccta aattgaatca
    27421 atggattatt ttagcgaacg cactagatca caacgattta aacaatctca aagcaatgta
    27481 tgagtcgtta gaaaatgaga acggcgattt aaaacagatc aatgccaaac tcatgctaag
    27541 cgatgtagca attaaacagg aaaatactgc attgaaagaa aaagcggata gtttagcaca
    27601 aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc aaagacattt cagaaattaa
    27661 agagcaacta aatccagctt caaagggagg tgagtagtat gtttagtttt agcgatgtga
    27721 aaatgatgta tgattggggc tgtttcacta acgaacaagt aatggttttc gttccgttgt
    27781 gcattactga agaaaaagca gataaaatca ttagcaaaga agagagcgca tcttaattga
    27841 tgtgcttttt attttgattc aaggagttgt cacatgatta atttagggga atggggagcg
    27901 atagcaggat caataaccgc tatcgtttct ttgattttat tagtaataaa accaattact
    27961 gcatctttct cgaagattac tgagactctt tcaaaagtaa gtcacaattt agatttgctg
    28021 actaaagatt tagaatcgag caaatcagat cgattgatga ttcatgaaga actaaagaaa
    28081 cacgatgaaa gattagatac acatgcagaa aaattggtag aacacacgca acaaattaaa
    28141 actttattta gggagagaag aaaatgaata ataaaacgtt cgaagtacta aaatggttcg
    28201 cactggtaat tattcccgca ctagctactt tcgtggggtt agttggtaaa gcgctcaatt
    28261 ggcagtacac agatatctgt gttgtcatca ttactggttt tggcgcgttt ttagggagtg
    28321 tgttgggtgt atcaaatcga acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa
    28381 acaaatgaaa aagaaaatta ctattactgc gatgagcctg ttaacggctc tttttttatt
    28441 acccattaat acgtttgctt atactattaa tgacgagtat aatttagcgc cgaatcaagg
    28501 agactccaga ttagcaattc ctaacaaaat tattttgcac gaaactggaa tagatgcacc
    28561 agcaagaaac gtagccgcca acatgaaaaa taattataac ggaagcaatc cttatactac
    28621 agatgttatt ggtgacggtg ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg
    28681 gggagctggt aacgctaatc cttatgcgcc tgtacagatt gaattacagc gcacatatga
    28741 taaagcattg tttgaaaaaa actatcgagc ttatattgaa tatacaagag atagtgcaaa
    28801 aaaatatgga attccattga ctcttgatca aggaacttct ttatttacaa aaggaatcat
    28861 ttctcatttg tgggtgacaa attatgtttg ggggaaccac acagatccat atggttactt
    28921 atcgcaaatg ggagttagca aagaaaagct tgcttatgat ttagctcatg gatttaccga
    28981 tgaaaatcca acaacttctg aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc
    29041 taatcctacg ctgacagatg gaacaaatca cgcccacatt gatcagtttg gggaaatcga
    29101 aaacgcaaac ttgcatgtgg ctggatggca cattgctaac tataaatacg agtatatttt
    29161 cattatggac tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
    29221 accagatgta aaccaagctt ataatacttc tggaaatgtt ggttatcatg tatctttcaa
    29281 tatgcgtaat tttcctagta agaaagtcta tgtaatgatg cgggcaacga atgatccaga
    29341 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
    29401 taaacgataa aaatagctcc tcgttgagga gcagtacata actatattga caactataaa
    29461 aattattcga taaaatagtg atgttatcgc atatcttcac tatcacccat aaatagtcac
    29521 actccaagct atgcgataac aggtttgttg ccacacattc tactggttga ttgtttatgg
    29581 ctttatgtgg caacaaccag tacccttagc tcagttggtc agagcagacg gctcataacc
    29641 gtccggtcgt aggttcgagt cctacagggt acattaacgt agccatttga atcgttctgt
    29701 gttagaattt ttttgaagag tattatacaa gctaaagctt ttcttcattg ccactcaaat
    29761 gagtggcttt tttatgtatc cttttatgga ttaatgaaag gatgtttcac atagttatac
    29821 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttaaa
//
LOCUS       DAXLPI010000022        28157 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXLPI010000022 DAXLPI010000000
VERSION     DAXLPI010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.022"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(220..1119)
                     /locus_tag="ACF3T5_000875"
     CDS             complement(220..1119)
                     /locus_tag="ACF3T5_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFD3631503.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1129..1485)
                     /locus_tag="ACF3T5_000876"
     CDS             complement(1129..1485)
                     /locus_tag="ACF3T5_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFD3631504.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1840..2136
                     /locus_tag="ACF3T5_000877"
     CDS             1840..2136
                     /locus_tag="ACF3T5_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFD3631505.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2780..3733)
                     /locus_tag="ACF3T5_000878"
     CDS             complement(2780..3733)
                     /locus_tag="ACF3T5_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFD3631506.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3756..4511)
                     /locus_tag="ACF3T5_000879"
     CDS             complement(3756..4511)
                     /locus_tag="ACF3T5_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631507.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4508..5467)
                     /locus_tag="ACF3T5_000880"
     CDS             complement(4508..5467)
                     /locus_tag="ACF3T5_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFD3631508.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5460..6410)
                     /locus_tag="ACF3T5_000881"
     CDS             complement(5460..6410)
                     /locus_tag="ACF3T5_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFD3631509.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6560..7012
                     /locus_tag="ACF3T5_000882"
     CDS             6560..7012
                     /locus_tag="ACF3T5_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFD3631510.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7101..9368)
                     /gene="gshAB"
                     /locus_tag="ACF3T5_000883"
     CDS             complement(7101..9368)
                     /gene="gshAB"
                     /locus_tag="ACF3T5_000883"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFD3631511.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9555..10553)
                     /gene="ftsY"
                     /locus_tag="ACF3T5_000884"
     CDS             complement(9555..10553)
                     /gene="ftsY"
                     /locus_tag="ACF3T5_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFD3631512.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10564..11373)
                     /locus_tag="ACF3T5_000885"
     CDS             complement(10564..11373)
                     /locus_tag="ACF3T5_000885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFD3631513.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11382..14963)
                     /gene="smc"
                     /locus_tag="ACF3T5_000886"
     CDS             complement(11382..14963)
                     /gene="smc"
                     /locus_tag="ACF3T5_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFD3631514.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14983..15669)
                     /gene="rnc"
                     /locus_tag="ACF3T5_000887"
     CDS             complement(14983..15669)
                     /gene="rnc"
                     /locus_tag="ACF3T5_000887"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFD3631515.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(16017..17798)
                     /locus_tag="ACF3T5_000888"
     CDS             complement(16017..17798)
                     /locus_tag="ACF3T5_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFD3631516.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17824..18738)
                     /locus_tag="ACF3T5_000889"
     CDS             complement(17824..18738)
                     /locus_tag="ACF3T5_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631517.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18765..19727)
                     /gene="opp4B"
                     /locus_tag="ACF3T5_000890"
     CDS             complement(18765..19727)
                     /gene="opp4B"
                     /locus_tag="ACF3T5_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFD3631518.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19727..20671)
                     /locus_tag="ACF3T5_000891"
     CDS             complement(19727..20671)
                     /locus_tag="ACF3T5_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631519.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20672..21676)
                     /locus_tag="ACF3T5_000892"
     CDS             complement(20672..21676)
                     /locus_tag="ACF3T5_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631520.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21944..22186)
                     /gene="acpP"
                     /locus_tag="ACF3T5_000893"
     CDS             complement(21944..22186)
                     /gene="acpP"
                     /locus_tag="ACF3T5_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFD3631521.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22308..23309)
                     /gene="plsX"
                     /locus_tag="ACF3T5_000894"
     CDS             complement(22308..23309)
                     /gene="plsX"
                     /locus_tag="ACF3T5_000894"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFD3631522.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23358..25394)
                     /gene="recG"
                     /locus_tag="ACF3T5_000895"
     CDS             complement(23358..25394)
                     /gene="recG"
                     /locus_tag="ACF3T5_000895"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFD3631523.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25511..27193)
                     /locus_tag="ACF3T5_000896"
     CDS             complement(25511..27193)
                     /locus_tag="ACF3T5_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFD3631524.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27245..27607)
                     /locus_tag="ACF3T5_000897"
     CDS             complement(27245..27607)
                     /locus_tag="ACF3T5_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFD3631525.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27877..28065
                     /gene="rpmB"
                     /locus_tag="ACF3T5_000898"
     CDS             27877..28065
                     /gene="rpmB"
                     /locus_tag="ACF3T5_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFD3631526.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7791 a         6087 c         4956 g         9323 t
ORIGIN      
        1 aataaaaaat gtatattata ttttttttgt gattatcgtt caaaaaaaga atacacgctt
       61 gcatctagta ctctgcgagt tacaaatcat ccgatcaaat aaacattcgc tgattatttt
      121 ccgcaaaaaa agcagcatat ccacgataaa tggatctact gctcttactt atccttatgt
      181 tttacaaggt attttctttt acacattact aatgctgtct caatacggaa cgattgtcat
      241 cgcttgttca aattcctctt gcaaatgttg tgtttgatag gcctgaaaag ctgggtgatg
      301 ggcacgcaag aggacacgta atttcatagg atcatagctg tctagcaact gttctttcaa
      361 gacaaaatct cgggtcagct gccattcata ataataacca aaaggtctgc agctgtaaat
      421 cgtgtgaccg atggatttat cttcaaagcg acgatgcgta tggccaaaaa ctacttgttt
      481 gatatttgaa taacggtcaa gtaattctcc gaatgattct gatcctaaaa atgcatttaa
      541 tttgttccaa cgctcataag gagcagattg atacatgata aatgcccgtt taggaacgaa
      601 atgagtggat aaaatcacct caagtttttt tgcttccagc tggtctagca aattttttgc
      661 agctgcaagc atttctgcat tgacctcgag atctgttttc ccacgctgga tgaaccgatc
      721 ataccaatac aaacgcttca ttcgtacgat tgcctcgtaa tctttcaatt cggaaaactg
      781 atagtcgtac cagccattca tccctaaaag aacggtctgc tcatttaaag caatataccg
      841 ttcatttaaa aaatgtgggt ctggaaaatg gtccatcatc tgttcattgg gcacatctgc
      901 caactcgtga ttgccgaaat gaaatgtcgt agataatcct ttattgacga aaaattcgac
      961 aacagctagg caacggtcta ctttattagc cgtatctccg gctagatgaa gccgagtgat
     1021 cttgtgcttt tgcaaaactt gccaaagctg catcagctct gattcgccga atttattgat
     1081 gtccacatgt aggtcgctga tgatcgccaa ttttcccatt ttatccctct agtcttttcg
     1141 aacaacttgc aagttgcctt ttctcaagcc gcttggtcct tttaattgta aactattata
     1201 agttggttct aaagcaagtg tggatggatc atccatgtaa agtattgcac tgtctttgat
     1261 tttgattggt ccaactggtg tatcagccac tacagaataa agacttagat ctgtttgtgt
     1321 atcttttttg acaatgatca atcgaaatgc cgtatccaat tggcaactga cttggctttc
     1381 tgcaaatgga ccgtcaccgt tttcgatatc taataaaagt tgatcgtctg cttctaattt
     1441 attcttcaat acttcttgtg cttcttttgt cacttctaat ttcatctgta tcacctcatg
     1501 tcatagaata atcactttat tcatcagatg caactaattt gtctggttct gactgttctc
     1561 aatttattgg aaagtaagtc attcgtttat tatgatttct cttatcacaa aagagacaac
     1621 tttccctttt cttcgttcag tttcttgaac ggaaaaagac ccaaaaagca acctgttatt
     1681 tgtttaacct tattcctttc ttaaataaca aaaaatacta taaaaaataa aaaaccttta
     1741 acaactattg gaaaaagaat tactttttgt tcacggatta ttaacaactc ttcggtagag
     1801 taaacttatc aaataaaaga cttctttgga ggaatcacta tgaaggaata tataaaaaac
     1861 atctatttta tcgaagaaac acaaaatatc gagggttctt atattgaagt aaaaacactc
     1921 tttgtcaatg aagataaaac caaagcattg gatatttata aaaaattagc ttcaaaaaaa
     1981 acgaatagtt ttggattgat attaagcgag tataaaatca aagcagaaga gagttatttc
     2041 tatcaattac taaaacggtg gagtaagctt cccgccgatt tttatcgaaa aatgcagatc
     2101 atcaactatc aaccgcttgc tgagacacat gcctaaatag aggttgtgaa atcagtgctt
     2161 tgagctaata ccgaaggtca gctgattgaa cattgtttag aagagattgt gacaactgcg
     2221 atttgctttg agtaataaga acgctggaca gaaaatagct tctcatattt tctagtctag
     2281 tgggcttatt gccgagaagc agcagatgga acatcgttta gaagagattg tgacaactgc
     2341 gatttgtttt gagtaataag aacgctggac agaaaatagc ttctcatatt ttctagtcta
     2401 gtggacttat tgccgagaag cagcagatgg aacatcgttt aaagaagaga tcggtacaaa
     2461 ataaaaatat ctaaatttaa gttttatcta agtttcgctt tgtacctttg attcaaatca
     2521 aaggaggcga tcttgatgct gaaaaaatgg atcacactat tttttgccac tccccattct
     2581 agaatcaacc cgtatgatgt agataatgaa ttcacgttga aaaaagattt ctcaaataaa
     2641 taaaaaccat ccgtaaaatc atcaatacga ttttacggat ggtttttgat aacttctttg
     2701 aggataggct cttgctattt aagaatgtaa gaaacattat tgttacatag tatgagagtt
     2761 taatcctctg ttatttatgc tatttaaatg cttgattcac atcttcgatc atcagtttca
     2821 ttgattctaa accgcccccg cttaaatacc agacatctgg ttcaagggag atgatctgct
     2881 gatttttacc agcattcgtt tgtgctacca gttcattggc agaaatatca tctttgctgt
     2941 cgtctccacc aatagctttt gttctgtcta ctacaaataa gatatccgga tttttttcca
     3001 gcacatattc ataagaaaca ctttgaccgt gagtggatgc ttcgatttga tcatctgctt
     3061 gttcaaagcc aaataaatca tggatgaaac cgaatctaga accagatccg taggctgata
     3121 attgcccttc attcacaagt gtgactagtg cttttttgtc agtagcttct gctttctttt
     3181 tcgtctgatc gattgcacta gataattcct ctaattgagt tttagcttct tcttgcttac
     3241 caaagatctc ccctaaagca gtgacatttt gctggaaaga ttcccatgga tttttcgcat
     3301 ctaaagaaag gaaaatggtc ggagcaattt gacttagatc tttttgaaaa tctctttgtc
     3361 ggccagaaat gatgattaga tctggctgaa gctgattaat tttttctaag tctggttctt
     3421 ttattccacc tgctgattct acttttttat aggatgaaag atactcagga aggctgcttg
     3481 tagctgctcc aactacctta tctccaacac caagagcatc catcgtatct aaagaaccat
     3541 tgtcgaatac gacgactttt tctggattat ttggcacctc gatttggtct ccattactat
     3601 ctgttatcgt caatgtagtt tcagtttgtg tgctgctttt ttttgtgtct gcctgactgg
     3661 tgttgttgct atttccacat gctgctaaac caaatgatgc tagaatcaag atacctacta
     3721 ctaatttttt catctcttct ctcctaatgt tagttttaga aataaagaca gaatctgcgg
     3781 ccttcaatct cacaaatccg tatgtccatt tcgtataatt catccaatac atcttttttt
     3841 atcatttctt gggtcgttcc ttgcgtaaat aactgtccat ctttcattgc aacaatttcg
     3901 tctgcataac ttgcagcaaa gttgatatca tgaagaacaa taacaattgt tttcccgatt
     3961 tcttgtacta aattttttaa aatctgcatg atttgaaccg caaaattcat atctagattg
     4021 ttcaaaggtt catccagtaa aatataatcc gtgtcttgcg ctagcaccat tgcaatatac
     4081 gcacgttgga gttgaccgcc tgaaagagtg tcaatcgatt gatccgctat ttcttctaac
     4141 ccaatgtgtt ccagtgattc ctgaacgatt tcctcgtctt ttgtcgtcaa ttttcctttc
     4201 gtataaggaa atcgtccaaa ttctactaat tctttcacgg tgatttggac atttatttca
     4261 ttcgattgtc gtaggatcga taattttttg gctaattcat tggatttcca agcctttact
     4321 tcgttatggt ctaaatagat ttcacctgta tcttttggta tcaaacgact gatcatcgac
     4381 aacaccgttg actttccagc accatttgga ccgataaaag cagtcaactt cttttcagga
     4441 atcgttaact gtacatcttt aaccactgat tttccatggt acagtttatt gaccttttgt
     4501 aaatcaatca taggatactc ttcctttcgc gaatcaattt tgtgacaaaa taaattcctc
     4561 cgacaaattc aacgacgaca ctcactgttg tattccagcc gaaaatctgc tctactgcga
     4621 gctgtcctcc tgtcagaaac agcatagcta ccagaaagct tccgataaat agctgaccat
     4681 gacgataagt agaaaagaca cgataactga tatttgcgac aataaaccct aaaaaagtaa
     4741 ctggacccac aagagccgtt gatgtccctg tcaaaagact gacacaacat aaaatcaaaa
     4801 attcaaaacg atctgtgtct atcccgaggt ttactgcttg ttcatttccg agatggaaaa
     4861 cgtctagcac atgtcgatat cgaaataaca ggtaccagca gccgataatg atcaaagaag
     4921 caggcaaaag atactgactc tggatattgc caaagcttgc aaataatctg ccttgaagca
     4981 aatcatattc attcggatcc atgatgactt gtaaaaaggt actgacacta ccaaaaagcg
     5041 tacctaaaat catccctgac atcagtaata aaaaaagatt cgctctcatt tttttcaaaa
     5101 gaaagaaaaa taacaataga ctcagcccga ccatcagccc aacgttcaat aagaaaaaaa
     5161 ctgtacctgt ctgctgaagc atcgtcactc ctccgataaa gaagaaaagg accgtttgga
     5221 caaggacata taacgactca agtcccaata ctgaaggagt caaaaactgg ttatgcgtaa
     5281 ttgtctgaaa gctgattgtg gcactgctga cagctagcga gacaaggata aatgcaaaaa
     5341 ttttcttact cctcaatgca catgcaaaat gccagtttcc atatgtatgg atcgtcaagt
     5401 acattcctgt aacaaataaa acaagaacaa gcataccaat aaaaatagat ggcttttttt
     5461 taagcatatc gtcgtcccct tatcagaaga taaaggaaga tagccccacc gatgattcca
     5521 acgatcacac tgactggtat ttcataagga aaaatcaaca cgcgagcgac aagatcgcaa
     5581 aatactaaga acaaactacc tgctaaagca gttacgccaa ttgttttgtg cacttgatct
     5641 ccatatattc tagagacaag attcggaatg atcactccga taaaaggaat gcttcctacc
     5701 atgatcaaca tgactgcact agccaatgat actaagccaa tgcctaaaaa ctgcatcttc
     5761 tggtaatcta ttcccagatt tactgcaaac gattcgccca gaccaatgat cgtaaatcgg
     5821 taagcaaata gccaaaccaa gagcaacaat ggaacaacag catacaataa ctcataattt
     5881 cctcgtatca cagtcgcaaa attcccttga agccatgaag tcatattttg taccaactgg
     5941 aattgataag caaaaaaagt agcaactgaa ccaatgatat tccctaacat gactccaatc
     6001 aacggaagca tcaattgatt tttctggagg aacaatcgcg taagggataa aaaaagcaat
     6061 gttcctgcaa aagcaaagca aaatgctgtc aaggatcgga caatcaagga tgcaccaggt
     6121 aggaaaatca tagccactaa aatacctaaa cgcgcactat ccatcgtgcc cgcagtcgtt
     6181 ggtgaaacaa atttattttg ggtcaaatgt tgcatgatca acccacaaat actactggtc
     6241 gctcccacaa ggatcaaaca aatcgttcgt ggcaaacgag tcactagcaa gatttgtaag
     6301 ctttgcccat ctaactggag cagttctgac aattgtacat tcgatactcc gatgaacaat
     6361 gaaatactac ttaaaataac taataatgct actagtcgtc cgtagcgcat ccaatccacc
     6421 tttctaattg agaatgattt tcattcttat tttaagtgaa tcgttgaaca aaagcaaccg
     6481 ctttttcata tgttttaggt attattggga atatgctata atcgtaagaa agacaaagag
     6541 aagaaggagg gtatttttta tggcacaaga tgatgtaaaa agccatttgg ataagggact
     6601 ttatggtaca cctttagtga accctgaaga acagcataaa tatttaggga cattcaggga
     6661 acgctgctat cttagtatga ctgttgctga aatgatccaa ccaaaaaaca aagaaaactt
     6721 tttaaaagaa ctcaaaaaac atccagatgc ttccatactc ttgaatgggg ctctacccct
     6781 ttctgtccaa aaccaatata tccagctagc aacgaaaaac aatagccgtt tcactgtagt
     6841 caacgattac gtcactgcaa atcctgagga attcggattg ctcttaacag caaaagaagc
     6901 agtaaatgaa cctgtgattg atatcgaaaa aaaataccca gaagaaaact cttcgccagc
     6961 tgaaaaagaa aaaccaaaaa agaaaaaaag cttctgggat cgtttatttt aaaacgaaaa
     7021 aacacataaa tttttacatg caaaaaaagc ttttgatcag attgacatca aataggacaa
     7081 tctgtcaaaa gctttttctt ttaaaatact tcaggatata acaatttcaa tacattcatc
     7141 gtcaatcttc tgccttttcc tgcaaaagga taaacatgca tatgcatggc tggattgaaa
     7201 tttgcttcga tgatcccata tgctttttta tcagttgtag gatcgatttc tttatctggt
     7261 ataatcaaat cgataccact aatcttagct cctagtgctt ctaccgcaga aacggcgatt
     7321 tttttgtaag cttcagaaaa ttcatcagtc atatcgatcg aatcaccacc tgtactgata
     7381 tttgagttct cccttagata aacaatttga ttggcttggg gaactgattc gatcgtcaac
     7441 ccttgttctt tcaacatcaa ttgttctaat tcacctaatt ggatcaattc taaaggtgca
     7501 cgatgattcg ttccacgcaa agggtcacta tttttttctt caacaagttc tttcactgtt
     7561 cggatactgt caccaataac attagcaggg actcgcaaca tgatggcttg gacttctccg
     7621 tcgatcacaa agaaacgata ttccgttccc ggcataaatt cttctactaa aaccgaactg
     7681 tcttcacgaa aagcaatggc taaacctgct tgataatctt ccagactagc tccttccttg
     7741 aaaatcgtga tacctaaccc gtaattggtt gatttaggtt tgatcacaaa tgcctgctcg
     7801 gcaaatctcg gataagcttt taccgcttct tccatggatg aaaattctgc gcctcctggt
     7861 acccggaacc cagcttcttt taagactttt ttcgttactg ttttgttttc catgataagt
     7921 gggacgatat agctgtcctt acttgtcatg tttgcatttt tcacatattc tacatggtct
     7981 tggaattgca gacgcaaaaa ttgatccgac tcatctaata ctttgacttg gacacctttt
     8041 tggatggcat cgaacataaa tatctgagta gaaagttcca tttcgcgaaa accggccaac
     8101 tgatagggac gttcatggct ttctttataa tattgatgtc ccaatgccac agcaacttct
     8161 tgctgactcg atagctgtgc ttcttgatag agtttcgctg ctaacgtttg ttcaggatga
     8221 tccatttgag tcaatgcttc ctctactaaa taaagagaat ccaaccaatc caattcaacc
     8281 agcatttgct tcattccttc aagaatttct ctaccttctt gatgaaattc tgtttgatct
     8341 gatggttgtt ctagtgctac tttgttattt cgagtcgtac cttctgctac ccattggtca
     8401 cagtcttctt tttcttctgt ccataacatg tacatcagga ataattgtaa gaaccgaact
     8461 tgctctggat tgatacccaa tcttgcataa ggattcaaat cgatattccg caattcaata
     8521 taacgaacac ctgttgttgc taaatctgcg actttctttc cgcctcggaa acgaagcggt
     8581 gtataaaatt ctttctcctc cgaaagtttc ccttcttcga tcattcgctc gatatctgct
     8641 aaatactgtt tcatagaagc ataggaaact ttcacatttg gatgattcgt atagcccagc
     8701 gcactgttgc gaatactgcg aacaggttct tgcggatgtg attcatctaa aaagtagttc
     8761 ttctcactca ttggtgaagc accgaataaa taagtgatca tccagcgata gcgcataaag
     8821 tttctcgctg ttttcaaata aagttctgtt ttgaattcgg aaaaatcttg aaattcttcc
     8881 tgatggctga acaatgttct tagcaactca tcgccaaatt caaaattgaa atgaataccg
     8941 cttaccatct gttttcgctt cccgtattct tttgctaaat aacgtcgata caagacatct
     9001 tcgaaatttt ttaattttgc aataatgatc tcttcgtctt tttccggtaa ggcaggtggc
     9061 atacttaatg gccagatcat ctcttctttc ggtatagaac gagcagtcac atcataaaca
     9121 gcagcgagat actgaaataa ttcgggaata gaatcggtaa caggggtgat catctctgtt
     9181 tgtgtttcgc taaaatctgt ttggatatag gggtgatagg atcgatcgcc aaagattgct
     9241 ggatgatcgg tttttgctag atttcctgca agatcaaccc gctgtccttc ccgttctata
     9301 ccaaatcgag cttggtcgat aaaagggcgc gcattgacat gcaataataa ttgcttaaaa
     9361 ttcatcatta agtgacctcc aattcatatg aatctaatta taatcaaaaa gtctaagaaa
     9421 aagaacgaaa ttgtttgaga accgcttaca aataagtata aaaatgaagg catcaaaaac
     9481 acagctatgt taaaaacaat cattcaaaca aggagctgca acttttgtca cagctcctgc
     9541 tttttagaca tgggttattc ttcttttagt aatcctttga acaatccaac gacaaaatca
     9601 tttggatcaa acggctccaa gtcatcgatt ccttcaccaa gaccgaccaa tttcactggt
     9661 aaatgcaatt cattacggat agcaagtacg ataccgccct ttgctgtacc atctaatttt
     9721 gtcaaaacaa gaccggtcac atctgttgtt tctttgaatt gttttgcttg aaccatcgcg
     9781 ttttgaccag tggttgcatc taatacaaga agtgtttctt ggggtgcttc tggtaattcc
     9841 cgctggatta ctcgttttat tttttctaat tcgttcatca agttcacttt attttgcagt
     9901 cggcctgcag tatcaactag taaaacatct gcctgctctg tctttgctct ttccaaagca
     9961 tcgaatacga cagctgctgg atcgcctcct gcattgccac gaacaacttc aacaccagcg
    10021 cgttcgcccc agacaactaa ctggtcgatt gctccagcgc ggaaggtatc tgctgctgcc
    10081 atcaatactt ttttaccttc gtttttgaac tgatgggcaa gtttaccaat acttgttgtt
    10141 ttccctacac cattaacacc gacaaacaaa ataactgtta gtccgtcttc ttggatattt
    10201 agttcattta cttcattgat tccttccgct tcatagagat ccacaagttt ctcgatgatc
    10261 gcattttgga cttctgccga ttttttcgca tttctgagtt tcacttcttg acgtaatgct
    10321 tcggtgattc gtatcgctgt ttcaaaacct acatctgcac cgataagtgt ttcttctaat
    10381 tcttcaaaaa agtcttcatc gactgttcga aaattggcaa acaactcatt catccgttca
    10441 ccaaatgttt tacgcgtctt ttctaaaccc ttgtcgtact tctcctgtac ttcaacttct
    10501 tctgttttct cttcgccagt aaaagcacgc ttgattttat caaaaaatcc catttgactc
    10561 cccctactct aatacaaatt tttggatcac tttggctacg ccattttctt cattgctggc
    10621 tgtcacgaaa tctgctgctt ctttgacgaa aggcacagca ttctccatcg ctacgcctag
    10681 tccagcatat tggatcatcg gtaaatcatt ttcctcatca ccgatcgcca tgatttcttc
    10741 ttgtttcaaa ccaagatctt ttgccaataa agaaatacca taagcttttg taacaccttt
    10801 tggcataaat tccaaaagca tacttcttgt tttgatgatc tcatacattt catggaattc
    10861 tgctggtatt tctttgattt taggatctaa ttcttcttgg tcataagcga ttactgcctt
    10921 attaagcaca aattcatctg taatatcgct tatatccgct ggttggaatt gcagcagctt
    10981 attcaacaca ttgtacaagg attgcttcct tggggcacta ggcagctgta aaaccacatt
    11041 gtcagacaaa acatctagtg gcagatccaa ttgctgagcc agtttgtata agcgttggat
    11101 atctgatact tccatcactt tcttttccat gatttcacca gtatcatttt tttgtaccaa
    11161 accgccgttg aaagtgatgc tgtagtcacc ttcatctacc agtcctaatt cttgcagata
    11221 atgcgccatc gcagctaagg ggcgtcctgt acatagaacg atcttgaccc cttgctgttt
    11281 tgcttgtgct aatgcttgtt tattctcatc tgaaatcagc ttttgttcat ttaataatgt
    11341 accgtccaaa tcgattgcta ctaatttgat cattttcgtt cctacacttt cgtttcgttg
    11401 attttcgcct cttcattgat atcttccatc cgtacagaaa cgatttttga tacaccggat
    11461 tcctgcatcg tcactccgta cagtacatct gctgcgacca tcgttccttt gcggtgtgtg
    11521 actacgataa attgtgtgtc atcttggaaa tcgctcaagt aacgaccaaa acgtttcacg
    11581 ttggcttcat ctaacgctgc ttctacctca tctaagatac aaaacggaac aggacagaca
    11641 cggataatgg aaaagagcaa cgcgatggct gtaagtgctc gttctcctcc agaaagcaaa
    11701 cttagacttt gcagtttttt gccaggaggt tgtacttcaa tctcaatacc tgttttcagc
    11761 aaatcagttg gttccgtcag tactaattca gctttaccgc cgccaaacat atttggaaag
    11821 acaattttga attcttgacg gatagcttca aatacttctt tgaatcttgt ccgcacttca
    11881 tcatccatct catccatcgt ttcaaacaat tggtttttcg ctgctaacag atcatctcgc
    11941 tgtgttgcta agaatgtatg tctttcactg acttgttcgt attgttcgat tgaatttaag
    12001 tttactggtc ccagtttttc aatctgttct tttaaggatg aaacctttgt tctactgtct
    12061 tcgatatcgg ttgtttcttg atagtcttgg gaagctttct caaatgtcag ctgatattct
    12121 gactgcagat aggtcaaatg attatctaaa atcatttcag ctcggtcttt ttggacttct
    12181 aatttggatt gttccgacag tcgtgctttt tgctcacgat tacgctctgc caagactgct
    12241 tcaagcttat cgatctcctt ttgccttcta tctcgctgct ctttggcttt tgccaactcg
    12301 gcttttaatg tttctcgctg agcagataat tcgttaattt gtttttccaa actttcttct
    12361 gtgacttcat gatcagaaaa gtctgctgtc aatgtcgcca actgcttttc tatcgcttct
    12421 tgacgttcgg ttgcctctgc tttttggact cgggcaccgc ggagctggat ctgcagatga
    12481 ttaaactgct ctttcaagac agcaagatct gcttgttctt gtgctttttg actttgtact
    12541 tgtgctcttc tggcttccat ctggtcgctt tcttgactta acgatttgat ttcttcgtcg
    12601 attttttgtc gttgcgattc aagatctgtt tgtttgtctt tcaattcgtt cgtttgcttt
    12661 tcgtaatctt cgataaactg ctgcagttca cgtgtctcaa aagttgagat ttgcttttct
    12721 ttttcaaaac gttcaagttc atttatgatg ttttgtagct gatttgtcgc ttcttgttct
    12781 tcaaaacgta attgttcgcc tcgtgtacgc aaaacttctt gttcttcggc taaacgagct
    12841 gtttcttgtt gcagctcctg aacttttttt tcttgtgctt ggagttgttt atctgcttct
    12901 tcaaattcac tagtcaactg tttcaattct tgattttgta caaacaaact gccttggttt
    12961 cctcgtttat tcgctccacc tgtcatggat cctccggcat tcatcacatc accttctaag
    13021 gaaaccactc gatattgata gcggatcgtt tgcgcgattg cattggcact agttaagtct
    13081 ttggctaaaa ggatcgttcc tagcaggttt tgaaccacag tttggatttg atcgggataa
    13141 ctgacttgtt cactggcaat ccctataaac ccttctacag ctgctgcttg tgtcaatata
    13201 tgagccggta attgtcttgg tttgattgtc gtcagtggaa ggaatgttgc tcttcctcct
    13261 ctttgctgtt tcaaatacgt gatggcttgc cgagcatctt tttcattttc aacgatgaca
    13321 tgctgggctg ccccacctaa agctgcttcg atagctaacg tgaaatctgc aggaacatcg
    13381 atcagttcag ccactgctcc gacaataccc gaaagctgct gtttatgctg caagaccaat
    13441 cgaacccctt ggtaaaaacc aaaatagttt tcttggattt cttgaaggct tttcttacga
    13501 gcacgtagct gctggacttg attcattaat tgatacattt taggctgttc ttttgctaat
    13561 gcttcttgga actttttccc gttttgttgg atcatttcta acttctcttt tgtttcagtt
    13621 aaagatgatt ttagctgcgc ctgcttttgt gtcaattctt ctttttgtaa gatcaatcgg
    13681 tctactgatg cttctacttc cgattgcttg gctaacgtct gcttgctttt tgctgtttct
    13741 tgtatatatt gacgttctag atacttcagt tcgtttccta cagctgcttg ttcttgcatc
    13801 aaatcaacat attgatctct taattctgcc aataattctt tcgttgattt ttggtatttt
    13861 tctaattctt gctgtgtttt tatgagattt gcttcagctt tttggatctc tgtttctttt
    13921 tctgctgctg ctttcatcaa gctttcttga agcttttcaa aatgtttaac ctttttttgt
    13981 gcttctgcta atgaagtttg atattcttgc gagctttttt gtgtatgctt tgtccgctcc
    14041 tgcaaaacat ctttttgtcc ttctgtctgc ttcagttttt ccgaaagatc taataatact
    14101 tgctgatttt tttcgatcag acgatcggct tgcgcatttt ctttacgctg cttcgccaaa
    14161 atggattctt gttcttggat cgactcagat agtttgccta attctagatt gaatttcgca
    14221 agctgtgctt gtttgttgtc ccaatctttt tttgctgtct tgatttcagc aaccatcaaa
    14281 gagacatcgg tttgagtcag tgtttctttt agccgtaaaa actcctttgc tgcctcactc
    14341 tgggcggcca aaggagttaa ctgttcttcc aattcgtgga tgatgtcttg gacgcggctg
    14401 aggttgtctt ctgtttcaaa aagcttctgt tctgctttct ttttccgctg cttgtatttc
    14461 aagacaccag ctgcttcttc aaagatccca cgacggtctt ccggtttact actgaaaatc
    14521 gcttctactt ttccttgaga aatgattgag aacgattctt ttcctaatcc agaatctaag
    14581 aacaattctt ggatatcttt taatctgcag ctttgtttat tgatgaaaaa ctcgctttcc
    14641 ccagtccgtc gataacggcg ggttacactg atctcattgt actccaatgg cagataatga
    14701 tcactattgt ctaaaatcac tgtgacttct gcaatattca gctgcttacg ggtatcggaa
    14761 ccggcaaaga tgatatccgg catttttccg cctcgcagac ttttagcaga ttgttctcct
    14821 aatacccagc ggatagcctc tgtaatattg ctttttccgc tgccatttgg tccaaccact
    14881 gctgtgacgc tattttcaaa atcaatgact gtcttgtctg caaatgattt aaaaccggct
    14941 atttcaattc gtttcaaata caaagctttc cctcacttac ttttacagtg ctgccaatgc
    15001 attttccgcc gcagcttgtt ctgctaattt tttcgatttc ccttgtccag tgccaatcaa
    15061 ttgatcatct acataaactt cgatccaaaa tactcgttca tgagctggac cttcttcatt
    15121 gatcaatcgg tattcgattg atacatcacc tgatttttgc aagacttctt gtagttttgt
    15181 tttatgatcc atctcatgtg aaaaagcacc tgctttgact ttaggaaaaa tcactttttc
    15241 gatgaatgca tgggtcgtgt cgaatccctg gtcaagatat aacgcgccta aaaaagcttc
    15301 gaataggtcg caaagcaatg ctggtcgcgt acgtcctcct gaattctctt ctcccttgcc
    15361 caacaaaaca tattgatcaa agtgacattc tttggcaaat ttcgacaagc tgtcttcgcg
    15421 aacgatcgca gctcgtgttt ttgtcaattt tccttctggc aaatgaggat ataaacggaa
    15481 taaatactga gaaaccatca gttctaaaac ggcatctcct aaaaattcaa gtcgctcatt
    15541 atccgataat tgcagattgc gatgctcatt cacataggat gaatgggtaa aagcttgttc
    15601 aagcaaattt agatcgtgaa aaacgatgtt atagttttct tttagttctt ttgtcagctg
    15661 gttgtccatt ttccacattc cttttatgga tattttttcg atacttcctt attatactgg
    15721 ttttttgtca aatttgaaat ggttcggtca aaaggtaagt aaagctttaa taatcctagt
    15781 gattgacgag aagttgtgaa aaattcgttt agtttcgagt atgaagaaaa aattttgtga
    15841 aaatggcttt ccatattttt gcataaattt gacttaatac cgagaaactg gactttgaac
    15901 accgtttatt ggagaagtca tgaaatcagc gccttgacct gaatattaga gaaataaaca
    15961 gaaaaatatc aaaaaaccga aaaaggacat cgcccttttt cggtcatttt tagtgattat
    16021 gaacgtgaat ctgctgtaac agaaacattt gcccatgggt ttggtacatc ataagaccaa
    16081 tcccagtctt tgacacggtc gttgactggc aatacttcat tacggaacaa cgttggaatc
    16141 gcaaatgctt cttcaaatgc atattgttgc catttttgat atgcttcttt ttggtactct
    16201 tcatcaaatg ctttgcttga actgatatcg ttgatcaatt ttgtattttc ttctgattca
    16261 taacgagtgt agttgaacgc agcatttggt ccccatagtc cacttggatt tggatcagtt
    16321 cctgtattcc aagcagcttg gtaaacatca atttcaggat catcgttttc taatttatca
    16381 tagaaagaat tgaattcgat cagacgacct gttgtgtatt ggacatccaa accaatttct
    16441 ttccattgtt ggatatagta atctgccaat ggttgcgctg tttccccacc ggacatcgaa
    16501 gcaaagttga ttgttagttt atttccatct ttgtcttctc ttaaaccatc gtcatccaca
    16561 tctttgtagc cagcatcatc taatagtttt ttcgctttgt ctagatcttg tgtgaatcct
    16621 ttcatctcat catcatggta tgactggaat actggaggaa tcaatgaagt cgcattgctt
    16681 ctcaaacctg catagaaacg ttcacctact gcattattat ctaatgcata gcccattgct
    16741 tgacgtaatg atttatcagc catttttgaa tctggattgt attccacact ctttgtatct
    16801 gcattccatt tacctaactt gaatcctaga taagtataag aagtttgttc acggcctaag
    16861 tttgtatagc cagcaatttc tttataggta tcgtaagtat ctgttggcat ttggaaaatc
    16921 atatcatata atttagagtt caatgcttca gttgcacttg ccatcggtac agatttcatc
    16981 gtgatctttt tcaatttagg cgtaccattc cagtagtgct cgtttggcaa gtattctact
    17041 gattctcctg caacgatatt actcatgtaa taagggccaa aagtcactgg atttttacgt
    17101 acttgatcgc ttgattccat atctttgatt gctacacctt ctaacgtatg ttttggtgct
    17161 gcatatgacc agataccacc acccgattga agcatgcttg ggtttacttc tttatattcg
    17221 atttcaactg ttttgtcgtc tatttttttg attccggaga ttgtgtcggc acttccatca
    17281 tgatattctt ccatacctac gatgttacgg aaagtgtcgt cataacggat accagtgtaa
    17341 tctttattcc caatgatttc gtaagggaaa atcacatcgt ccgccgttac atcttcacca
    17401 tctgaccatt tgacgttgtc ttttactttg attgtcgctt ttttcgtatc ttcatctaga
    17461 tcaagcgttg cagctccgtc gtctgtgatt ctgaaatcgt catcgtttgc aaatagcggt
    17521 tcatgagaag gagccataaa agctgcatca tatgaatctt ggtaaaactc ccactggaac
    17581 aatcctttaa actgactgtc cgttgcgacc gcagcttcta attcgccgtc ttcgattgca
    17641 tcatcattat tttttgttgc aattggaaaa gttaattcct ctttgttatt gccgttgcct
    17701 ccagagttgt ttccagaggt atctgtacct ccgcccccgc atgctgcaag aacgacagca
    17761 gacatcagcg ctgcaaatcc aaaaatcgtt ttcttcattc ctatttcccc cttcgcttct
    17821 atactagcct aaccgttggc gcgcatctgc agaacgcttg aatgcttgtc caacataatt
    17881 gatactcaac atcaaaacta aaataaggat tgatgcgggt aaccagatcc attgccgatt
    17941 cactaagact tctccgttac ttgcataacc gatcaatgta cctaaactag gtacatttgc
    18001 cggcaagccg aaacctaaga aagtcaaagt tgtctcgata ccgatgtttg ccgcaaagtt
    18061 catcgtcaaa ttagtaatga ttaatgaact tagattcggc atgatttcgc ggaacatgat
    18121 tttaaaatca ttggttccca ttgtttttga ggcactgaca taatctcttc ttgcttcaga
    18181 taatgtcttg cttcggaata atcgggcttt agctacccaa taaaatgcac tcatgatgaa
    18241 tataaaggac cagacattgt atctggaaat aatcgatacg aagacgataa tgatcagcat
    18301 gatcggtaga atcatgataa aatcaacgat ccgcatcaag atgttgtcaa tgatccctcc
    18361 gtagtagcca gagatgatcc ctaatccaac cccgatgatc gaagtaagga tcgtgatcgc
    18421 aaatccgatc gtaacagagt ttctagcacc tatgatcaat tgtcctaata catcacgtcc
    18481 gccttcatct gctcccagaa tgaacttttg tccttgtgtc gaaactgttc ctggtgcagc
    18541 gtatttgtcg aagatactaa catacatcac tttgtcttga tcggtcagca tcgcaccgat
    18601 aaagatcact aataataaaa ccactagcaa acctaatgaa aaaatagcaa ttttatcttt
    18661 tttaaattct cgagcaatca ttcgtatccc cataggagga atactttctt gaactgctgt
    18721 attgtttttt tcactcatcg atccattctc ctttctgatt ggttttattg aatccgaata
    18781 cgcgggtcaa cgatactcat gatgatatct gaaagtaatg tccccactag cgtcgcgatt
    18841 cctaaaatca atactaagga tgtgatcacg gtatagtccc gttggccgat ggaatcaata
    18901 aatagttttc cgactcccgg ataaccaaaa atcttttcga taactacaga accagcaatc
    18961 aacgaagtga tttcataccc catttgagaa gccatcggca acgctgcatt tcggaaaata
    19021 tgtcgactga atactttatt cgtcggtacc cctttggaac gggctgttct gacaaaatcg
    19081 agcgatttcg aatcgattac ttcacttcgc agatattgga ttgtgacggc agtcgccaaa
    19141 aatgcttggg taattgatgg taacagaata tatttcaaac ggtctatcca ttgagtgata
    19201 aatccacctt gcaaacctag tgtttgtgat ccacttgttg ggaaccagcc taaacgataa
    19261 ccaaaaacaa atagcatgat caagccgaag ataaataaag gaacagcaaa actaaagaag
    19321 ttgtagatta cgacaatctt atccaagatt gaattttgat accggccagc aagcatcccc
    19381 agaggcacag caatcaaata agtaaggaca actgtcaaga tcgatagata taaagtgttg
    19441 atgatccgtt cgccgatcaa gtcagcaaca gagcgtttgt ataagaaact ttgaccaaaa
    19501 tcgccttgca aagcatttcc gatccaacgg aagtactgga cataccatgg gtcattcaag
    19561 ccagctgctt gtcgcatctg ttcgatcact gccggatcct gatttggatt gatcaaaccg
    19621 gtaaatggat cccccggcat cgcttgggct aataagaaga tcagaacact taaaataata
    19681 acttgcggga tcatcaataa aacacgtcgc agaatcgttt tccacattag cttgcacctc
    19741 catctttcaa cgccactttg tgtgtagttg tgatcgtacg caaatcgtag actcgaccag
    19801 tcgcatcata gtattctttt tggttttgga tatactcttg ttctactctt cgtcgatttt
    19861 ctttgtggct ttccctattt tctacatcga tttgcggaat agctgaaagc agacgtttcg
    19921 tatagatatg tcgcgggtca ttataaatgt cttctcttgt accgatttct acaaatcgac
    19981 ctttgtacat aattgcgata ttgtcgcaca tgtgttttac tacacctaaa tcatgtgaaa
    20041 tgaataaata gctgaggccg tattcttgct ggattcgttt catgaaattc aatacttgcg
    20101 cttgaacaga tagatccaga gcagatacag gttcatcggc tacgatcagt tttggattgg
    20161 tagcaacagc acgggctact cctagacgtt gacgttgtcc gcctgaaaat tcatgaggat
    20221 atttatatag cgcatcttcc ggcatcccaa cgatatctag cagtcctttt acctttttct
    20281 tttcttcttg atcgcttaac cgttcaaaat ttcggatggg ttcagcaatg atatccaaca
    20341 ctcgcttttt aggattcaaa ctggacattg aatcttggaa gatcatctgc acatccttgt
    20401 tataacccat ttttttgcgg ttgcttcgct tcgttacatc ttttccttca tacatgattt
    20461 gtccggctgt taccttttct aatcccacaa cggcttttcc cgtagtagat tttccagagc
    20521 cagactctcc cactaaacca tatgtctttc ctttttcgat aataaaatcc acaccatcta
    20581 cagcatatac atgatccgtt actcgattga agaatccact gcgaataggg taatgaactt
    20641 tcaggtcttt gattgtgatt aattctgtca tctacacctc tcctttttcg tctctaaagt
    20701 ggaaatgctt atagcaggag caacgtacca agtggttcgg tgcgacttca tgaaggatag
    20761 gctcttcttc atgatcgctt gctggtatcc atggaatacg tggtgcaaac cgacaacctt
    20821 tacgaggcat gtttttcaga gaaggaacga caccttcgat cacatggagt tctgcatcat
    20881 gatttccttc ttgtggtata gagttcagta aagagcgtgt ataaggatgc ttaggatgat
    20941 caaacagttc ttttacacct gctacttcaa cgaactgtcc gccatacatc actgccactc
    21001 gatcagccat ttctgctacg actcctaagt catgagtgat caagatgatc cctgtttttg
    21061 tttcttcctg aaggtctttc agtaaatcaa gaatctgtgc ttggatggtt acatctaggg
    21121 ctgtcgtcgg ttcatctgca atgacgattg gaggtttgca ggcgattgcg attgcgatga
    21181 tgacacgttg tctcataccg cctgatagtt catggggata ttggcgtcca actctcgcag
    21241 gattaggaat ccctacttgt tctagcaatt ctagtgcacg agcttgacgc tgttctgcat
    21301 tcatctttgt gtgataggaa agtccttctt tgatctggtc ttcgattctc atgagaggat
    21361 ttaatgcaga caacggatct tggaaaatca ttccgatatc atttccgcgg attttattat
    21421 agagcgtttc gttcaggtcg attaaattca gatcgttata taaaatctca cctgtaattt
    21481 ttgtgttatt aggatcaagt aatcccataa tcgtagtagc caaggtactt ttcccgcacc
    21541 cagattcccc aacgattgct aaaatctcgt ttttgtctaa agtaaatgaa acgtcatcta
    21601 ctgcatcgta atagtcatct ttgagtcgga atcctgtatg taggttctga acttctaata
    21661 attggttatc ggacaatcga atacccctct ctgcttccta aatggaagtt tttcttaata
    21721 aaaaaggaca ttacacagaa taaatcgttt tataacaaag gttattctta tctttgtgta
    21781 atgaattatt aataattata tagaaaaccg ctttactttg caacataatt tctgtttatc
    21841 gccagaatta tcagaaaaat gacttttacg tgtcaaaaag attaaaaaaa gaaggataaa
    21901 aagctgcatg cagcttttta tccttctttg cttttatttg aaattattta agatggctgc
    21961 tgatataatc tactgcgcca ccgactgttt caatttgttc agcgtcttca tctgaaattt
    22021 ccgtaccgaa ttcatcttca agctctaaaa cgaattccat aatactaatt gaatcagctt
    22081 ttaaatcatc tttgatgttc aattcatccg tcactttatc ggtatctatc tcaaaatgat
    22141 ttgaaataat tttcgctact ttgttaaata cttcttcgcg agtcaacttt ttcacctcca
    22201 agaatcatac acttgtcaat tacctacacc atggctaatt ttactggcat ttttcaggaa
    22261 tgtccacctt tttttcaatg gaacggatcg cttcagatat ccaaatacta ttcttcgctt
    22321 ttttcaaatt gttcgactaa ttgtccaact acatctgtct caagcattgt gtggatttgg
    22381 cggatcgtat agcgaacagc atcggggcct gttgctccat gagttttaat gactggtgct
    22441 ttcaagccaa acaaaacggc accgccatgt ttggaataat ccatttctgc tttcaaagaa
    22501 cgtaagccgt cttttaaaag catagcgccc atttttcctt tgatcccttc atttagaata
    22561 gcagatttta atagattcat catattcatt gctgttcctt caatagattt caaaacagcg
    22621 ttgcctgtaa atccatctgt gaccactaca tcagccacac cattgagtag ctcacgagct
    22681 tctacgtttc caataaaatt caagcttgtt tcattctgaa ggagttcata ggcttttttc
    22741 gtcacttcac taccttttgt tgcttctgtt ccgttgttta agagtgcgac acgaggacgt
    22801 ccgatacctc ggactttacg tgcataaaaa gaacctaaaa taccgtattg caacaaatgt
    22861 tctggtttgt tttctgcgtt agcgccaaga tccagcatat cgaaaccggc accttctttc
    22921 cctactactg gcaaggtaga catcaagcca gggcgttcga tatttttgat acgtcctacg
    22981 atgaacaacc ctgctgctag tagcgcgcct gtattccctg ctgaaaatac agcatctgct
    23041 tcaccgtttt tcactgcttg tgcagcaagg accattgatg cagtcttttt tcgtcgaatt
    23101 gcttttactg gctcatcatc actattgatt ttttcgtctg tatggataat cgtgatgttt
    23161 ttttcatttg ttaagtattt cttgatttct gcttcttttc cgtaaagtaa gaattctact
    23221 tctggaaaat cttgctgcgc taaagccacg ccttctacaa ttgcttgagg tgcgtgatct
    23281 ccgcccattg catctacggc aattttcatt gtttccactc ctttttccgt catcagtata
    23341 aagtatagca cagtctgcta atcgaaataa cgattatcct gattttctat ttggaggctt
    23401 tgcgctaaag gctgatactc aggaagcatc tgccaatcct ttatttgcca aagggctgca
    23461 gcctcttctc tggctacctc caaaacattt ccatccgtca ccaagtcagc aatcgcaaat
    23521 tgaggcagac cagattgacg gaagccaaat acttcaccag gaccacgtaa ttccagatct
    23581 ttttcgctta gtacaaaacc attggtggtc tctgtcatga ttttcatccg ttccacacct
    23641 agttcatttt taggattagc aactaagata cagtaggaag catcacttcc gcgtccaaca
    23701 cgtccacgaa gttggtgcag ctgtgccaat ccaaatcgat ccgcatccat gatcagcatg
    23761 acggtcgcgt tcggcacgtt tacccctact tcaattacag tagtcgaaac taatatctgc
    23821 atctggttgt ctttgaaggc ttccatgatt acttcttttt cctgattctt cattttacca
    23881 tgtaacaagc caacttcata ttttggtgca aatagttcac gaagtttttc atagatttcc
    23941 actgcatttt ttacatctaa tgcttctgat tcttcaatca gcggacagat gacatacatt
    24001 tgatgtcctc ttgccagttc ttttttcgtc caatccagta cactgtccaa ttgaggtgtt
    24061 cttacccagc gtgtttcgat cgggatacgt ccagctggaa gttcatcaat gatagataca
    24121 tccatctcac catatgctgt aatagctaat gttctaggaa tcggagtcgc tgtcataaat
    24181 aagacatctg gtcgccagcc tttttcacga agtactttcc gctggttcac gccaaaacgg
    24241 tgctgctcat ccgtaataac tagacctaaa ttctgaaatt cgactccatc ttggatcaat
    24301 gcatgtgtgc cgatcacgac gttaatttcc ccttgctcta gtttttcaag gagctcacgt
    24361 cgttcttttg ctttcgttga ccccgtaatc aatgcgatgg ttacttcatt gggatcaaac
    24421 agctgttgca agctttccat atgttgttgt gccaagattt ctgtaggcac catcaacgca
    24481 ccttggaaac ctgctgtaac ggtagcgtat aacgcaatcg cggcaaccac tgtttttccg
    24541 ctacctacat ctccctgcaa aagtcgctgc atatgctgcg gactgcgaag atcccgacag
    24601 atttcattcg ttacacgttt ttgtgcattt gtcagttcaa aaggtagctt ttgagtaaat
    24661 tgtttcaatt tttggacatc ataaaggatc tccaaaccat tcgtttctgc tttttctgct
    24721 tttttcaatc cttgaagacg catttgaaat aaaaagaact cttcaaaaac gacacgtcgt
    24781 ttggcttgat ggttttcttc gtggtctttt ggaaaatgca tacttcttac agcagaagct
    24841 cggtcaagca gtcgatattt ctccacaagg ttgtttggca gattttcttc aatcaatgtg
    24901 ccgtattctt gaaacccttt tttgattaga tcaactaatg tcgtttgacg aattgatttg
    24961 ttgacgtgat atatcggcga aaagtcatcc attgtttgag aacctaaaat cttcatcccg
    25021 ttcaaggctt tacgctttgc atcccattta ccgtatacgg cgatttcctc cgataaaatg
    25081 actttttctt ttagataagg ttgattaaaa aaagaaacgt tgaacacatc atgttcttgc
    25141 atcatacgaa attgcaatcg gcttttttta taaccaaatc gattcatcac aggaggcgag
    25201 actacgattc ctttcaaagt aaccttttct tgatcctgga tctcgtttag atttctttcc
    25261 tgaatatctt cgtagcgaaa aggataataa gtcagtaagt cttcaatcgt ttggatgccc
    25321 aagctggcaa gactagctgc tcttttttct ccgactcctg ccagcatagt gattggatca
    25381 tctaaagaac gcataaattt ttcctccctc ctttaacaaa aaaaggactt ggtcagcgat
    25441 catacctgat cactaaccga aggcctttta tgaatgaata ctgtgttaga aagatattct
    25501 gacacagaaa ttattcagca gaaaatagat aaggatagac aggctgatct ccttggtgga
    25561 tctcgatttc caaatcagga tgtttttctg ttagtttctc agcaaattca tttgcttgtt
    25621 caactgttcc ttcatcacct gccaagatcg ttacgatctc tgtgtcctca ttgatcattt
    25681 gttctagtgt acttaacgct gttgctgtga tatctgggtt agagatcagg atttttccat
    25741 cgatcatgcc caaaaagtct ccttctttga tctctacacc gtcaattgag gtatctctga
    25801 tcgcgtgagt gacttgaccg ctcacaacgc tagacaacat ttctgtcatc gtttctttgt
    25861 tttcttctaa agaagcttga tcattgaatg aaagcatagc tgtcaatcct tgggaaatcg
    25921 ttcttgaagg aacaactgct actgggatat ctgctacttc tgctgcttga tcagctgcca
    25981 tgaagatgtt cttattgttt ggcaagacaa tcactttatc tgcgtttact tcttgaatcg
    26041 ctttaacaat gtcttccgta cttggattca ttgtttgtcc accactgata acataattag
    26101 cgcctaaact ttcaaacagc tcttggacgc ctttaccagc agcgatcgct acgataccat
    26161 acgggatacg tggtatttct tttttcgcaa actcgttcac ttcttcatcg tgttccaaaa
    26221 tcgtctcatg ctgcaagcgc atgttgtcga ctttgatttt gactaatgaa ccgaattttt
    26281 gtccataatt caagacttct cctgggtttt ctgtatgcac gtggactttg atgatctcgt
    26341 catcattgac cacaagtaaa gaatctccta taccatctaa atagttacgg aattcttcgt
    26401 aatcgaatgt actatcgact gtaggtcctt ctccgatttt taccatgatt tctgtacagt
    26461 aaccaaaatg gatatcttct gtcgccattt gaccttggac actgcgatga tgttctgcat
    26521 tgaccatatc atccatttct gctggagaag gttcatatgt ttcatccgct tggaattctc
    26581 cattcaatgc acttaaaaag ccctcataaa caaacagcag tccttgccct ccactgtcta
    26641 cgacaccgac ttcttttagg actggaagca agtctggagt tttagctaaa gcacgctttg
    26701 ctccttttac aacagctgtc attacttcaa cgacatcatc agtacttttt gctttctttt
    26761 ctccagcttt cgctgcttct ctagctactg tcaaaatcgt tccttctact ggtttcatta
    26821 ctgctttata cgcagtttca actccatgag tgaaggctgc agcaagatca gaagcattta
    26881 atgagacaac atctggaatt tgcttagaaa atccacgaaa caattgagac aaaataacgc
    26941 cggaatttcc gcgcgcaccc atcagcaatc cttttgaaag cattgctgct aattctccta
    27001 ctttttctga ggttgaatct actactgcct tagctccact agtcatagat agattcatat
    27061 ttgttcccgt atcgccatct ggcacaggga atacatttaa tgaattgacg tattctgcat
    27121 ttacttgcag acgattcgca cctgcctgga ccatttcctg gaactggctt gcacttattt
    27181 ctgttacact cacctagata atcctccttc agatcgactg ctgagttacc agccagtctc
    27241 ctgcctagtc tggcagtaca cgaacacctt gtacaaagac attaactgaa ttggcagtta
    27301 cgcctaataa tgcctctaga ttgtatttca ctttttcttg gacgttgcgg gaaacttcag
    27361 agattttcgt accatagctg ataatcgtat aaacatctac agcaatacca ttatcttctt
    27421 ggcggacaac tactcctctt gagtaatttt ctttacgcaa aatctcagta atattatctt
    27481 taatttgatt tttgctggcc attcctacaa caccgaacac atctgtcgcg gcgccaccaa
    27541 caactgttgc aattacatca ttggtaattt caatggttcc agctgacgtt tgaattttta
    27601 cagccatgaa tgagcctcct taacgggtaa aaaactccca tttttcttct atcatatttt
    27661 atcatagcgc aacttgaaat aaaaggcagt tacccaaagt actcaaaaac cttgcaaaat
    27721 ttcaggtata accttgttta atttacaatt attcttctca attgtcaatt gtatctatga
    27781 aaatgtactt gcaaattccc attctatatg atatgttagt ctggtatgag ctgaattatt
    27841 ctattgctcc aaatcaaggc aaaggaggaa attaccatgg caaaagtttg ttactttaca
    27901 ggtcgcaaga caaaaagtgg aaacaaccgt tcacacgcta tgaactcaac aaaacgtact
    27961 gttaaaccta acttacaaaa agttcgcgtt atggtcgacg gaaaacctaa aaaagtttgg
    28021 gtgtcaactc gtgctttgaa atcaggaaaa gttgaacgcg tttaattgaa tgatacttgg
    28081 accgttcaga tagtgaacgg ttttttattt aataaaaatt tttaagggtg ggacttttga
    28141 ttcctaccct ttttttg
//
LOCUS       DAXLPI010000023        27697 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXLPI010000023 DAXLPI010000000
VERSION     DAXLPI010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.023"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            267..2033
                     /locus_tag="ACF3T5_000899"
     CDS             267..2033
                     /locus_tag="ACF3T5_000899"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFD3631527.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            2135..3394
                     /locus_tag="ACF3T5_000900"
     CDS             2135..3394
                     /locus_tag="ACF3T5_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFD3631528.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            3541..4842
                     /locus_tag="ACF3T5_000901"
     CDS             3541..4842
                     /locus_tag="ACF3T5_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3631529.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            4845..5696
                     /locus_tag="ACF3T5_000902"
     CDS             4845..5696
                     /locus_tag="ACF3T5_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFD3631530.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            5709..6524
                     /locus_tag="ACF3T5_000903"
     CDS             5709..6524
                     /locus_tag="ACF3T5_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFD3631531.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            6675..7841
                     /locus_tag="ACF3T5_000904"
     CDS             6675..7841
                     /locus_tag="ACF3T5_000904"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3631532.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            8071..8718
                     /locus_tag="ACF3T5_000905"
     CDS             8071..8718
                     /locus_tag="ACF3T5_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFD3631533.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            8802..9746
                     /locus_tag="ACF3T5_000906"
     CDS             8802..9746
                     /locus_tag="ACF3T5_000906"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFD3631534.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            9881..11332
                     /locus_tag="ACF3T5_000907"
     CDS             9881..11332
                     /locus_tag="ACF3T5_000907"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFD3631535.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLIRSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            11353..12189
                     /locus_tag="ACF3T5_000908"
     CDS             11353..12189
                     /locus_tag="ACF3T5_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFD3631536.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            12189..12848
                     /locus_tag="ACF3T5_000909"
     CDS             12189..12848
                     /locus_tag="ACF3T5_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFD3631537.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            12974..13663
                     /locus_tag="ACF3T5_000910"
     CDS             12974..13663
                     /locus_tag="ACF3T5_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631538.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            13669..14625
                     /locus_tag="ACF3T5_000911"
     CDS             13669..14625
                     /locus_tag="ACF3T5_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFD3631539.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            14669..15181
                     /locus_tag="ACF3T5_000912"
     CDS             14669..15181
                     /locus_tag="ACF3T5_000912"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3631540.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(15437..15985)
                     /locus_tag="ACF3T5_000913"
     CDS             complement(15437..15985)
                     /locus_tag="ACF3T5_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFD3631541.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(16074..17255)
                     /locus_tag="ACF3T5_000914"
     CDS             complement(16074..17255)
                     /locus_tag="ACF3T5_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFD3631542.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(17491..18999)
                     /locus_tag="ACF3T5_000915"
     CDS             complement(17491..18999)
                     /locus_tag="ACF3T5_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFD3631543.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            19158..19349
                     /locus_tag="ACF3T5_000916"
     CDS             19158..19349
                     /locus_tag="ACF3T5_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631544.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            19552..20469
                     /locus_tag="ACF3T5_000917"
     CDS             19552..20469
                     /locus_tag="ACF3T5_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFD3631545.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            20462..21535
                     /locus_tag="ACF3T5_000918"
     CDS             20462..21535
                     /locus_tag="ACF3T5_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631546.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            21683..22201
                     /locus_tag="ACF3T5_000919"
     CDS             21683..22201
                     /locus_tag="ACF3T5_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631547.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            22182..22574
                     /locus_tag="ACF3T5_000920"
     CDS             22182..22574
                     /locus_tag="ACF3T5_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFD3631548.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            22650..23585
                     /locus_tag="ACF3T5_000921"
     CDS             22650..23585
                     /locus_tag="ACF3T5_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFD3631549.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            23718..24497
                     /locus_tag="ACF3T5_000922"
     CDS             23718..24497
                     /locus_tag="ACF3T5_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFD3631550.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVFFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            24490..24807
                     /locus_tag="ACF3T5_000923"
     CDS             24490..24807
                     /locus_tag="ACF3T5_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFD3631551.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            25051..26661
                     /locus_tag="ACF3T5_000924"
     CDS             25051..26661
                     /locus_tag="ACF3T5_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFD3631552.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            26818..27666
                     /locus_tag="ACF3T5_000925"
     CDS             26818..27666
                     /locus_tag="ACF3T5_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFD3631553.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         8971 a         4893 c         5557 g         8276 t
ORIGIN      
        1 gaatatactt ctacgttaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt
       61 gatcgcttaa gataaattca ggacactaaa tagtacagga agtagattaa aacttttaaa
      121 agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt
      181 taccgataac atttttgaaa gcgttgtctt ttgtttctct atcacttata gtgatgacgt
      241 catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa
      301 gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa
      361 atgatatcaa aaaagtatat gtgctttgtg gagatccata taccataaca actgaaaaat
      421 ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg
      481 aaattgaagt gacgagcgaa acaaggcgct tacagtatgc gttccatgtt gttggggaag
      541 ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta
      601 ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc
      661 cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg
      721 acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag
      781 acttcttcgg gggagatttg caaggtgtga gggatcattt ggattacttg acggaccttg
      841 gcgtcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata
      901 cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga
      961 ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg
     1021 gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag
     1081 actggttcca catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa
     1141 ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata
     1201 cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat
     1261 tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa
     1321 aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc
     1381 attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattca
     1441 cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg
     1501 gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat
     1561 tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga
     1621 agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag
     1681 aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg
     1741 aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt
     1801 tgtctaagat tatttctgaa ggttctactc agtggctaat agtatatgat cgtgaagata
     1861 agctctattt cacacgggaa ctagagggac agatcatcta tgtttacttt aatcagtcaa
     1921 aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt
     1981 tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt
     2041 taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg
     2101 taacagcacg ttgaatacac aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt
     2161 gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa
     2221 ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg
     2281 atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat
     2341 caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc
     2401 tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca
     2461 aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc
     2521 ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt
     2581 gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct
     2641 atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc
     2701 taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt
     2761 tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg
     2821 aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa
     2881 aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga
     2941 cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat
     3001 tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt
     3061 cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca
     3121 ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag
     3181 ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac
     3241 agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt
     3301 gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa
     3361 aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat
     3421 aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc
     3481 tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag
     3541 atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt
     3601 ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata
     3661 ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt
     3721 actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg
     3781 caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat
     3841 cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata
     3901 cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg
     3961 atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac
     4021 ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt
     4081 atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt
     4141 tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct
     4201 caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg
     4261 gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc
     4321 atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc
     4381 tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta
     4441 ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta
     4501 tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt
     4561 cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat
     4621 aatttctcca tgatctatct gtttaacaat ggtggtcccg gaagcgtagg cggaggagct
     4681 ggttctactg atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag
     4741 tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg
     4801 ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt
     4861 cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac
     4921 tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa
     4981 atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc
     5041 tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa
     5101 caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt
     5161 ttatcggccg caaatcaagt ttgatgttct ttttagtgat tcagatggta ccgactatgg
     5221 cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt
     5281 tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat
     5341 actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt
     5401 tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat
     5461 tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac
     5521 ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa
     5581 gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct
     5641 tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc
     5701 agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag
     5761 tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag
     5821 tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat
     5881 tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg
     5941 aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg
     6001 aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg
     6061 attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata
     6121 cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac
     6181 gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt
     6241 taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt
     6301 tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa
     6361 atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc
     6421 actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat
     6481 gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt
     6541 atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa
     6601 aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt
     6661 taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct
     6721 cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca
     6781 ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta
     6841 acgcttcttt ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta
     6901 tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt
     6961 atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc
     7021 atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt
     7081 attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag
     7141 tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt
     7201 gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga
     7261 tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag
     7321 aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc
     7381 atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca
     7441 aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac
     7501 aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc
     7561 ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg
     7621 aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag
     7681 tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt
     7741 tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg
     7801 ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt
     7861 taaagactat ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac ttttttgtct
     7921 aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca taataaaagt
     7981 ttggttcttt aatttttaaa catgacagaa aattttttct tcgttatact tgtaatagaa
     8041 tactatttta taacaaaggg gatcgaatca atgaaaaaaa ctgttattta tactagttta
     8101 tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac atctgggaat
     8161 acgtcttcca gtagcgaaca gcgctcttca caggtatcgg actcctcaac aaaagcaagc
     8221 acatcttcgt ctagccagac aaaaagttct agtgaagcaa gtactcagtc agatatcaaa
     8281 gtgtctgcag ctcgtgcgat cgaattgttc caagataaat acccagatac tgcaattact
     8341 agtttggaat tagatagtga ttggggatct tacttctaca agatagaagg tgtcgatgat
     8401 caaaaagaat acgaagcaag aattgatgct caaaatgaaa aaatggagac agagaatcca
     8461 gaaactctag atcgagatga acaaaatggt caaaagaaaa aagaagatgg tctggatgtt
     8521 gataaattga tcacgattga ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt
     8581 acggcaacag attgggattt ggataaagat ttaggtacta cttattggga agtcaaagta
     8641 aaagacggca atcaaagtac atcagtcaag atcaatagcc aaacaggcga agtactgtca
     8701 actgaacaag atgattaaga atttcttcgg aaaacaagag ttgatttttc ttttagtcat
     8761 gatacactag acaaaataac ttgtttgaag gagcgattta cttgagttta gaaacgacct
     8821 ttgccaatca tatctttgcc aatcctttga tgaatgcatc tggtgtccac tgcatgacta
     8881 cccaggaatt agatgaactg gctcattcag aagcaggagc atttatcact aagagctgta
     8941 cgatcaatga acgaaaagga aatcccgaac cacgctattt tgatgttcct ttagggagta
     9001 tcaattctat gggacttcca aacttaggtt tttcttatta tctagagtat gctcttgcgt
     9061 atgaaaaagt gcaggaaaac caaaatcaac cgttattttt ttccattgcc gggatgagtg
     9121 tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat gggatcactg
     9181 aattaaattt atcttgtccc aatgtgcctg gaaaaccaca actagcttac gattttgagg
     9241 ccacttatga aacactgaaa gaagtattca gcatcttttc taagccgcta ggaatcaaat
     9301 tgcctcctta ttttgatttt gctcattttg atcagatggc tgatatcttg aatcagtttc
     9361 cattgaccta tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc gatacagaac
     9421 aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga gagtatatca
     9481 agccgactgc tttggcgaat gttcgcgctt tttatacacg tttgaaacca gaaatccaaa
     9541 tcattggtac aggtgggatt cggacaggac aagatgcatt tgaacatctg ctatgtggcg
     9601 caagcatgtt acagattggt acagagcttc ataaagaagg gccggaaatt ttttctagaa
     9661 tcataaaaga attaacgcag attatgtcag aaaaaggata tacgtcaatt gacgaattta
     9721 aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg tcatgattga tcttcgtctt
     9781 tttttataaa aaacactcgt ctgttcattc ctgtgcaagc gtactcattg tatgttagac
     9841 gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat atgaatgatg caacagaatt
     9901 agcaaaagag cttaaagaaa aagaatttag ttttcaagaa ctagttacgg atatacagaa
     9961 aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag
    10021 taattcccac gagctattga ttccccagtc agaaaaaggg ccattggctg gtatcccatt
    10081 tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt
    10141 atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg
    10201 tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc
    10261 agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag
    10321 tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg
    10381 cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg
    10441 agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt
    10501 cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca
    10561 ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg
    10621 gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt
    10681 acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat
    10741 tccttaccca ctagatggtc gtaaactgat caggtcttat tatcagatga atggcgcaga
    10801 aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga
    10861 acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta
    10921 catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga
    10981 atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt
    11041 gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg
    11101 tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt
    11161 ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat
    11221 gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg
    11281 ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca
    11341 aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt
    11401 ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa
    11461 aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat
    11521 gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta
    11581 aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa
    11641 agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc
    11701 tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat
    11761 tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact
    11821 agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg
    11881 aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat
    11941 tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac
    12001 gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa
    12061 aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct
    12121 gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg
    12181 agtgagtgat gtactttcct ctaattgatt tttccctcat gtggacttca cttcctttcg
    12241 tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatt
    12301 tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta
    12361 ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat
    12421 atttcgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta
    12481 ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc
    12541 agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt
    12601 tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga
    12661 aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca
    12721 taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg atttattggg
    12781 tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atccatcagt
    12841 caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt
    12901 gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc
    12961 ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata
    13021 ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat
    13081 tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga
    13141 gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta
    13201 taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg
    13261 aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac
    13321 atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac
    13381 aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg
    13441 cgtaaaatat tttccaaaag atcaattgta caaagaattg aaaagacgtt cagaaaatca
    13501 agatccattt tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc
    13561 aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca
    13621 acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct
    13681 tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg
    13741 ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat
    13801 gtgatcgaat tatttcaaaa aaagcaccat ccaatcgctg tgatccaaaa tccccaagta
    13861 ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct
    13921 gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca
    13981 gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg
    14041 gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc
    14101 gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca
    14161 gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt
    14221 ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga
    14281 gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt
    14341 gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat
    14401 aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt
    14461 gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg
    14521 tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa agattggctt
    14581 aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat
    14641 gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt
    14701 agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc
    14761 tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa
    14821 agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat tagtctcagg
    14881 cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt
    14941 atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt
    15001 cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac
    15061 ttatccagct aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg
    15121 gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg
    15181 actataaaat catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat
    15241 gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa
    15301 aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa
    15361 ctcccttcat cgagaggtcg tgacatagct tttgtcacga tctctcggct tatcctacga
    15421 gtattattcg atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga
    15481 acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg
    15541 ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt
    15601 ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg
    15661 tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc
    15721 ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt
    15781 ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa
    15841 aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga
    15901 tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac
    15961 cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa
    16021 agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt attttatacc
    16081 agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc
    16141 aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa
    16201 atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt
    16261 gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt
    16321 gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg
    16381 ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat
    16441 atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca
    16501 gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat
    16561 gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga
    16621 tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca
    16681 tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata
    16741 gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg
    16801 gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat
    16861 ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga
    16921 atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg
    16981 gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct
    17041 tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta
    17101 ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt
    17161 agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg
    17221 acaaataaac caataatacc taataaataa ctcaaataat cactcctatt ccaaattccg
    17281 ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt cattactttt
    17341 ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata aaaggtttaa
    17401 aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa gctaatagtt
    17461 tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt cggaagggta
    17521 gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat catttttatt
    17581 gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat cataagcaac
    17641 ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt cattgatgct
    17701 tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg tttcaagaac
    17761 taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta ctgcatttaa
    17821 gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga tgacttcatc
    17881 tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg cttgaatctt
    17941 atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta aaccgacttc
    18001 ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc
    18061 gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt ggacaacggt
    18121 atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag attgataaac
    18181 agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg tctcaacatc
    18241 ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat cgatcatatt
    18301 gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat gtttcaacgg
    18361 aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc ctttttggct
    18421 ttcatgatac agttggttca agcaagtagc gagctttcca cttcctgggc caggagctgt
    18481 cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac catcttctga
    18541 gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat agaccttgat
    18601 gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac tatagcgagt
    18661 gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta accgcaagat
    18721 atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa tatctccagc
    18781 atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt tgattttcgc
    18841 atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta acttcccacc
    18901 aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg ctgactgctc
    18961 ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc cttaagtact
    19021 aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa
    19081 acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat gatagttttt
    19141 gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat aaacactata
    19201 tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg gaacaattga
    19261 cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag cgcattcatt
    19321 tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg gattcgattc
    19381 tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca
    19441 gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag aaaatggtac
    19501 tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag tatgaaagag
    19561 ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaacgc ggatcaggat
    19621 acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat gaatctttcc
    19681 caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag
    19741 atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc aaatgaaaat
    19801 cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca ggaatttcct
    19861 tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat cggcacttat
    19921 taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt tgttttgcct
    19981 aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta tgacaaaaaa
    20041 atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg tttagatgaa
    20101 caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg attcttgaat
    20161 cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt actatctaat
    20221 gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca gcaaaaagaa
    20281 gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa
    20341 aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat ccatgaattc
    20401 ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg aggttccgag
    20461 tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag aagatcatga
    20521 agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca cagaagaaat
    20581 ccctatgcta gaggaaacaa aaatacatga aaaagaaaaa gtaactccga cgaatacgaa
    20641 tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt tagaaggaaa
    20701 agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag aattagcgga
    20761 tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag aatcatatga
    20821 tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc tgatcgaata
    20881 cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt caaattcagc
    20941 ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg gacaaaacga
    21001 attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg aatcgatgag
    21061 tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc gggtgctttc
    21121 tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca cgatattcaa
    21181 tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg ttaatcagac
    21241 attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg aattgacgaa
    21301 tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa
    21361 gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa cgataacaac
    21421 aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac gatctgtcat
    21481 tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt cctgattgga
    21541 taagttggat taccagatgt cattcagata attctgttcc aaaggacttg cggcagttat
    21601 ccagaataat tgtcacaagt cttttctgta tgtactaaat ggacgtgaat aacttgctga
    21661 tgaaggaggg atttcattag gaatgtccac aaataaaata gcacagctta gcttattgtc
    21721 tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc agccaatgac
    21781 tgctattttt ttatttatag tactttactt aagcttaaaa gatgcgttaa ttgtcatgag
    21841 tctttctatt gcgatcagca gtttttattt tggtgttgga ccatgggtca tcggccaatg
    21901 gatcagctat actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca
    21961 aaggaatcta tggttaaagg gagtctgttt ttttctggcg ggcatggttt acggaatagg
    22021 tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa ccttggatct attaccttca
    22081 aggggtgtcg tttgatttga tgcacagtat aggcaatgtt gtgttctttc ttgtttttct
    22141 gccagtcgtc aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa ataatttata
    22201 gtttgcttgc aatgagtgtc agtatactca ttttgacaag ttgcagtaca acctatacac
    22261 aatcggcttc caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca atcacgttac
    22321 aagaaaatgg tcaaaacttt tcggaaaaag aagtggtctt caaaaaaggc gatgatttac
    22381 tgagtatttt gaaacgaaat tttgaagtca aagaagataa cggcttcatc acttcgcttg
    22441 aagggcactc acaggatgag aaaaatcaac tctattggat gtttactgtg gacgggaaaa
    22501 gtgctactac tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc attttcaatt
    22561 tgtctaaact ataagtttca taaaaaactg ttaaagggcg aatattgcca taattcagtg
    22621 agttcagttg atgaaagaag gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt
    22681 ccgtaattgc tgctctaggg gctaatattc tagttgctat cagcaaattt gtcggctatg
    22741 caatcagtgg cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt gattgcagca
    22801 atcagatttt gctgttattc ggtgataaac aagccaataa aggacagagc gagttgcatc
    22861 aatttggtga aggacgagca aaatactttt tcagtacaat cgtcgcaatg atgcttttct
    22921 tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa gttgacgcat ccatcccatg
    22981 aagtgggtaa tccgatcatt gttatagcaa tactgttatt tggtttggta atcgaaggaa
    23041 gttcgttacg cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa ctttccacga
    23101 tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat tatttttacg gaagacttgt
    23161 gtgctgtaat cggtttggtc ttggctttag caggaacaat attgacttat gcaaccggga
    23221 ttgctgcatt tgatgcgatc agcggattgc tgatcggttt cttgctgatg ggagccgcca
    23281 ttttcctagc aaaagaattt tatagtctga tcatcggtga aagcgtgaca gcaagcgatt
    23341 tatcaaaaat caagttagct tttgaacgtc ctgagatcag tcggctgatc gatataaaaa
    23401 cagttcattt aagtccaacg gagattttag ttgcagcaaa agcagatatc gttggtaaca
    23461 aagagaatga agcttactcg gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc
    23521 ctgacaagaa gatgtatata tacattgaaa cagataaata tgacccaaat tattctcgta
    23581 aataaaacgt ttattcatga attgaagcaa aaatgagaaa aatataatct tgtttttaaa
    23641 taaaacggct acaatttagc gaatttatgt tacaatgagt ccgctgttta tttgttgtaa
    23701 ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg
    23761 cagtgatgcc actttgtata agctatatac cagtaggatt agcttgcgga gtattgttgc
    23821 aaaaagttgg atttgatcct ctcttatcag gactgttatc gattctggtc ttttccggtg
    23881 gcgcgcagtt tttagttgca tctatgctta cgacgcaagc atcatttgca acgacattat
    23941 tgatggtttt ctttttagaa ttacgctata cattattagg agcaagtctt gccggtttta
    24001 tgaaaaaaga aaagcggacg tttttggctg tcttttctca atcactgaat gatgaaaact
    24061 atgcggtgaa ctacttaaag ttttcaactg acccttcctg gaataaaaga aaggctttat
    24121 atgtcaattg gttctcaatg actgcatggg ccggtagcaa tatgatcggt aattttttcg
    24181 gctcggtgat tcgagtagat gctgaccttg tccattttgc tttgacggcc atgttcattt
    24241 tcatgtttat catgcagatg aaaagcgcac tactgatctt tacaggttta ttctcaggcg
    24301 tattaggggt cgtattcatg gtatttttcc aaaatacttt cggattgatt gcagcaacag
    24361 tcattgcttc atttgccgga tttatgctgg agaaggcatt caaaaaagaa aaagctaaga
    24421 aactaagaga ccgcggtaaa gatgtcaatg agccgctctt caacttcccg gaacaggagg
    24481 ccaatcacca tgactaaact atatttgcta cttttagttg cttgtctgtt catcgtggca
    24541 tatatcccgc gcttgttccc gatgctttat tttactcatc gaaaagtgcc atcctggttc
    24601 agtgactgga tgaaatatgt accggtggca ttatttgcgg cgttagcttt taaagatgtt
    24661 ttcattactc atgaacatct ggatattgct tggaatatca aaatagctgc aatgattcta
    24721 gtagcaggcg tggcttacaa aacacgttcg atggcgttgt cagttttgac aggacttgcg
    24781 tctgtctttt tgctgtctat gctttaaact gttttatttt attaaaaaca gagattgcga
    24841 cataagttgt tcaaactgat ctatccgaac tgcatttgca acagtcaatt ctggatgcaa
    24901 gtttattcgt agggataaaa aagattagtg ttggcttatg atgcaagctc ttttttattt
    24961 taggaattag agagggattt gtccttctat gattttttgc tataataggg aagatgttta
    25021 ttttatgaaa gaatagttga gggaatttaa atgaataaaa aattgatgca aggaacattt
    25081 tggctaacct ttgctaatct tctatgtaaa gttttagggg tagtctattt gattccctgg
    25141 ctaagtatga tggggaacaa tcaagatggt atgcttgcta cgacattgta taatgttggc
    25201 tatcttcctt atggattgtt cctgatgctt ggtacagtcg gatttccaaa tgcgattgca
    25261 aaaaaagtag ctgtagccac aaaagaagga gataacgctg cttgtcggct gatttttaga
    25321 agtacgatca atatcatgtt tgtcatcggt atcgcctctg ctgcactgat gtatttattt
    25381 gctcctttac tagccggaat cagtccgatt gctaacgtta ataatgggat actagctatc
    25441 agaagtttat gcccatcttt gattgctatc ccaattctta gtgcaatgag aggatatttc
    25501 caaggaaaaa atgatctgcg gccttatgga acttctttga ttatcgaaca ggctgtgcga
    25561 gtgatcgtta tcttggctgg tacttattac ttgcgcgtat tgactgacgg aaccatatta
    25621 gaagctgtat tgatcagtac agtcgcttct ttcttcggtg ggttagctgc gattatccat
    25681 atgtatatcg tcggacgcag aaaagacttc ttcgaactta aagatttttt gatttccagc
    25741 cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta tcattcggga aacactaccg
    25801 tttatttttg tcggctcagt cattactatc gttcaaatga tcgaccaagt aacgatgaaa
    25861 ccactgcttc actttttccg tccagagatc gcagatcagc aactggaatt cttattcagc
    25921 cgtgcttctg tcaatccaaa taaattgaca ttgatcctga tctccatggt aggaacagta
    25981 gctataagca gcttgccaat attaagcacg atgaagaaaa aagatcggat acaaatcgaa
    26041 aaaacagtag gagatagttt ttcgattgcg ttattgattc ttcttccttc attgactgga
    26101 atgtctttat tagctggacc actatacaca ttgttttttg gctatgatcc tgaaagtgtc
    26161 ggatatttcc aaatggcttt gctagcttct ttgtttttct ctttattcac gatcctatct
    26221 acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc gattaactgt agaagcaatt
    26281 atcctgaaag tcatcttcca agtaatcgga ttaggattag ttggcggata cgggatgagc
    26341 ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa
    26401 gaataccgaa tagcaccttt agcaaagatc agcaattttt tcttaaaaac tttccgctcc
    26461 accctgatta tgcttgcagt atgttttgcc gttttctttt tgctgaatca gcgattgtca
    26521 atggaatcaa aaagctatgc aatgatttat tgtgtggctg taggaagtat tggcggctta
    26581 gtatttttat tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat
    26641 catagcagaa gcaaagaata ggttaactaa tcgttttttg ctgtcacttg aaaaacggca
    26701 actcgtatga ttacgcttgt cgtttttcta ttttccaatt ttagtttcat atctaatctt
    26761 acttatgata tagttataag gtctggaaaa attctttgaa acgaggaaat taaacacatg
    26821 aaacttgcag tagttacaga tagttcggca tatctgccgg aacgtatcaa gaaccatccc
    26881 gacttattcg ttattccaat ccctttgatt atggatggga aaatttataa cgaaggaatc
    26941 gatatcgaag cggatgaata ttatggactt ttaaaaaaca gtaaagattt tcctactact
    27001 tcacaacctg tcgtagggga agttttgggt ttatatgagg aattgaaaga gaaagggtat
    27061 gacacgattt taagtatcca tctttcttca gggatttctg gctttgtcaa cacattattt
    27121 gcgatgaaag atgacatcag cggagtaacg gtcattcctt atgactctaa gatcactagt
    27181 atgccgatgg gacacatggt agaagctgcc ttagacttaa atgatgaagg tcagtccctt
    27241 gatgaaatac ttgcacatat cgatcgaatc cgagataata cctatgctta tctgattgta
    27301 gatgatttga acaatttagt acgaggagga cgtttgacaa atggtgcagc gctgattggc
    27361 agcttattaa aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt tttattcgaa
    27421 aaaatccgtt caagtaaaaa agcatttgct cgagctgaag aaattattgg tagaagaaat
    27481 aacgaaatac aaccgccagt caaactttac gtgatacatg caaataattt agaagtagcg
    27541 attgcggaga aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga aattggtcat
    27601 ttcggaccag tcattggtac acatttaggt gaaaaagcca tcggcattgc catttcagcg
    27661 caataattcc ttttattcaa aaaacatacc aaaaata
//
LOCUS       DAXLPI010000024        27102 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXLPI010000024 DAXLPI010000000
VERSION     DAXLPI010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.024"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(58..510)
                     /locus_tag="ACF3T5_000926"
     CDS             complement(58..510)
                     /locus_tag="ACF3T5_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3631554.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(975..1343)
                     /locus_tag="ACF3T5_000927"
     CDS             complement(975..1343)
                     /locus_tag="ACF3T5_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFD3631555.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            1493..1975
                     /locus_tag="ACF3T5_000928"
     CDS             1493..1975
                     /locus_tag="ACF3T5_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFD3631556.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            2012..3022
                     /locus_tag="ACF3T5_000929"
     CDS             2012..3022
                     /locus_tag="ACF3T5_000929"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFD3631557.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(3137..3850)
                     /locus_tag="ACF3T5_000930"
     CDS             complement(3137..3850)
                     /locus_tag="ACF3T5_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631558.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(4023..4630)
                     /locus_tag="ACF3T5_000931"
                     /pseudo
     CDS             complement(4023..4630)
                     /locus_tag="ACF3T5_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            5114..5341
                     /locus_tag="ACF3T5_000932"
     CDS             5114..5341
                     /locus_tag="ACF3T5_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFD3631559.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            5357..6523
                     /gene="hemW"
                     /locus_tag="ACF3T5_000933"
     CDS             5357..6523
                     /gene="hemW"
                     /locus_tag="ACF3T5_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFD3631560.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            6757..7800
                     /gene="hrcA"
                     /locus_tag="ACF3T5_000934"
     CDS             6757..7800
                     /gene="hrcA"
                     /locus_tag="ACF3T5_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFD3631561.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            7894..8457
                     /gene="grpE"
                     /locus_tag="ACF3T5_000935"
     CDS             7894..8457
                     /gene="grpE"
                     /locus_tag="ACF3T5_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFD3631562.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            8508..10337
                     /gene="dnaK"
                     /locus_tag="ACF3T5_000936"
     CDS             8508..10337
                     /gene="dnaK"
                     /locus_tag="ACF3T5_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFD3631563.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            10488..11654
                     /gene="dnaJ"
                     /locus_tag="ACF3T5_000937"
     CDS             10488..11654
                     /gene="dnaJ"
                     /locus_tag="ACF3T5_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFD3631564.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(11736..12365)
                     /locus_tag="ACF3T5_000938"
     CDS             complement(11736..12365)
                     /locus_tag="ACF3T5_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3631565.1"
                     /translation="MGQLSDNQQLCQERINPLLELLERVFSYYGQALLTVHRQQIIIL
                     VNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSFLHAKQVCEWIAF
                     HQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLFKKTLACFSKNNG
                     SIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLLVQT"
     gene            12377..12550
                     /locus_tag="ACF3T5_000939"
     CDS             12377..12550
                     /locus_tag="ACF3T5_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631566.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(12595..13332)
                     /locus_tag="ACF3T5_000940"
     CDS             complement(12595..13332)
                     /locus_tag="ACF3T5_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFD3631567.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(13334..14836)
                     /locus_tag="ACF3T5_000941"
     CDS             complement(13334..14836)
                     /locus_tag="ACF3T5_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFD3631568.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            15095..17089
                     /gene="uvrB"
                     /locus_tag="ACF3T5_000942"
     CDS             15095..17089
                     /gene="uvrB"
                     /locus_tag="ACF3T5_000942"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFD3631569.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            17099..19915
                     /gene="uvrA"
                     /locus_tag="ACF3T5_000943"
     CDS             17099..19915
                     /gene="uvrA"
                     /locus_tag="ACF3T5_000943"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFD3631570.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            20178..21443
                     /locus_tag="ACF3T5_000944"
     CDS             20178..21443
                     /locus_tag="ACF3T5_000944"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFD3631571.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            21447..22178
                     /locus_tag="ACF3T5_000945"
     CDS             21447..22178
                     /locus_tag="ACF3T5_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFD3631572.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            22313..23197
                     /gene="rapZ"
                     /locus_tag="ACF3T5_000946"
     CDS             22313..23197
                     /gene="rapZ"
                     /locus_tag="ACF3T5_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFD3631573.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            23194..24192
                     /gene="yvcK"
                     /locus_tag="ACF3T5_000947"
     CDS             23194..24192
                     /gene="yvcK"
                     /locus_tag="ACF3T5_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFD3631574.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            24226..25161
                     /gene="whiA"
                     /locus_tag="ACF3T5_000948"
     CDS             24226..25161
                     /gene="whiA"
                     /locus_tag="ACF3T5_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFD3631575.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            25704..26354
                     /locus_tag="ACF3T5_000949"
     CDS             25704..26354
                     /locus_tag="ACF3T5_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631576.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            26541..>27102
                     /locus_tag="ACF3T5_000950"
     CDS             26541..>27102
                     /locus_tag="ACF3T5_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HFD3631577.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT         8934 a         4570 c         5721 g         7877 t
ORIGIN      
        1 aaaaaaatag cgctagaatt tccaacatct ctggaatttc tagcgctttt taattgttta
       61 agatacaagg gaaatatctg tctggatcaa taatttttca ttgatccatt gactaaagtt
      121 tacgacataa ttgaagttac caccgattag tatctccgca tgattgtctt tataaagatc
      181 gtattgtaaa agacggtctt ttttatttgc gtaaacatcg ttatggtact ggactaattg
      241 ttctcggaat tgattgattg ctgtaaaatc acgatattga aggttgaaac gcatcgtgat
      301 tggtgcattt agcatgaatc gctgcatttc ttttgctgct tcctctgctt gatctccgta
      361 taggaactca aaaagattac gttcagaaaa tttccattgc ccgccaacct ttgttccttt
      421 tagttttcct tcatgaagat aatttctgat agaacgtgta gtaacaccta attgttcggc
      481 taattcattt actgagtaaa aatctttcaa tgttcaccct cctatgtgaa tggttctact
      541 gacttagttg atatttgctt aatcacgaaa tgtagaaaga acgattttaa ttaaggtcat
      601 tgtaaaaatc aagtcagaaa attctaactt ttatttctct aaagaaattc gttctctcct
      661 aaagtgatat tatatttaaa tatacctgaa aataatgggc agtattatga aagatcactg
      721 atattttctc gtattttttc cttattcaaa agttttgcag gaaaattgct gaaaaaataa
      781 ttttactaag tagaaaattc aaagccattc aatcagtgat ttttttcagc acaagaaaga
      841 ctccttataa aaatagcaga tactttgtca attaagtgtt taaccaggga tgataaaaag
      901 gataggattg aacaaaacct gtataaggtg atgaatagtt tcctcgccag taaaaggttt
      961 attcgtataa agctttatcg ccaaaattta tcatgacctt ttttatcttc aatattaaat
     1021 gaaatcaatt gttcaatcaa ttcccagttg attacacttt tccaaggaac tttgatgacg
     1081 ttatccgtat atacgtagcc tgcttcttgg atattttttt caagggcacg aatcgctgct
     1141 ttttccggtg cgatagaaaa atgattttta gcaaaactaa atgcaataat aaaggtacca
     1201 tggtcagtaa acataggttg gttccatttt acagttgtgt ctagctgggg atacgtttct
     1261 gttatcttag caaaaaggtc tttgatcttt tgttgatgtt ctttttcttt gatttgattg
     1321 atgatcggct gaaatttttc cattctaaaa cgcctccatt aagtggtttg atttatttta
     1381 ccagaaaaac aatagttatg tataaaaaac gaacaaaaca caagtaatgt tgtaaccgct
     1441 atatttatat gataagatgt ctcaaataat aaaatagaaa agagacataa aaatgattga
     1501 cttggcaaag aaattcttac acgaaaatat atattttgag ttgtatcatc atcgagccat
     1561 ttatacaaat gaagatgctt tggtaataaa gaaagaacaa ggatttacag gtacagagac
     1621 caaaagctta ttcttaaaag ataaaaaaga taataactac gtatatctga cttttactac
     1681 gaaacaaacg gatttcaaag cattagggaa gatcgtcggt aaaaggcttt ctattgtttc
     1741 atcagaacgc atggaagaac aaacgggaca aaagcagggc gctgtttctc cgtttggtta
     1801 tgaaaaacag attccgatta ttattgatga agagctctta tcaaatgaaa aacttgtttt
     1861 tgcaccaggt agaccagatc aaacgatggt catccaaacg gttgacttaa acaaaatcat
     1921 ccagctactt gagatgccga tatttttgat gccggcaaac gaaaaaaaca attaaccaag
     1981 taaaatagtg catacttgga ggtgaagcaa catgataagt tatccattat tagaaagaaa
     2041 tgcaacgatt ggggttactg caccatcctc aggcgtatca gaggaacacc attcgttact
     2101 cgaatcagct ttaaagagaa tggaagaaaa atctttccaa gtagttactg gaaagacgct
     2161 ttggacgcaa gataaggaaa aatcaggtcc agcaagaaaa cgagcagatg aattcaatca
     2221 aatgatgcaa gatgaaaata tccaaatgat ctttccgact tggggaggag aattattgat
     2281 tgagattctc gattttgtcg attttgatgc tctacaacca aaatggatag taggttattc
     2341 agatattagt acgttgctac ttgctgtcac attaaaaaca gggatagcca ctgcacatgg
     2401 aactaacctt attgatttaa gaggagagta cttggatgaa acgacagcaa tgtgggaaag
     2461 tgtactgaaa acaagagaag gagaatcgat tctacagcaa tcttctgaaa agtatcaaaa
     2521 agtgtggccc catgagcatt catcatcttc tgttttccat ctcactgaac caacaaattg
     2581 gcaatcagtc agcggagatg cagtaaggat gaagggacga ttattaggtg gttgtattga
     2641 tacgatcagg catttaatcg gtactcctta tggagatgtc caatctttta aaagaaagta
     2701 taatgatgga aagccgatct tgtggtttct agaaaactgt gagctatcgt cagttgattt
     2761 aagaagatca cttgttcaga tgaaactggc aggctggttt gatggcagct cggggatcat
     2821 gttcggtaga agtgcggcag aggatttgtt agattataaa atgaaggatg tatacgaaga
     2881 tctgtcaaag gaattaaaac taccgatctt gtatgatatt gattgtggtc atgtaccgcc
     2941 acaactgaca ttgattaatg gtgcctatgc agaagtagcg gtagaagata gaaaagcgac
     3001 tgttttgcaa acgtttattt aaaaaatttt ttcctgcctt ggagtctatc aaagaccaat
     3061 aaatctaaat agaaaagaaa gtgaaagaag ctgacaaaat gctgtcagct tctttttgta
     3121 taacatatta gaaagtctaa ttatctataa ctgtcacatc gattaactgt ttcacgtcat
     3181 tgggatttcc tgtaggaata gctactacag atgcattatc aatgatttct tgagttgttt
     3241 ctctaatgaa cgctggataa gatgtttgat ctgcaggcat ttgccaatgt gaaggaactg
     3301 gataactagt gattgtcccg tcatggttac ttgaatacac taaaactccc tggtaactgt
     3361 atttcccagt gagcataacc acatctttcg gatagactgc actccctgca tcataagggg
     3421 cgataggtgt tcctgcagga taatgaataa catgtaattc gaaatcaccc gatgtatctc
     3481 taaaagtatt acctctaata gctagccaaa cacgtgcgta ctcaatttgt agatcagaat
     3541 atcctgctaa cggatctatt gactgagaag actgtgagga ggttgtgtct attgttgaag
     3601 aagaaatatt tgtctcaact gtactactag atgtagaaga agtattgggc tctgagactt
     3661 tactgcttgt tgaagatgta ttcgtttcag tagaagctga tttctgagta cttgattttt
     3721 cagctgaact ggtttgtctg attgaacttt tgtctgtggt cgcagatgga tcactatttt
     3781 ctttactagg agacgtacat cctacaagga ataaagagac caacaataaa ataaaaccta
     3841 actttttcat ataaaatacc tcattatttt tttaatacca gtatagcata atcctgaatt
     3901 tttgaatccg taaaaccttg tctatgaata taaatagagt gtatcttctt tgataaatgt
     3961 cagaaaaagt gtgacaaata atcatcagta aaattacttg tcacactatt gataaggagt
     4021 atttaattga agagaatgat taggagaaag gatctccgta ctttttatag taatcataga
     4081 atccaagttt gtatagacga atcgtttttt ctcttatttc ttctgtttca aaatggactg
     4141 cttgaagaat caagtctgtg aattctatag gagctgttcc attcgcagta atcaaattac
     4201 gatctttcac agcttgttgt tctataaaat cttctttatt ctgataattt tccgattcta
     4261 accaaggaaa ttgcgcattt ccggtgtgtt tgtaaccttg caataatccg ctctgagcta
     4321 aataatcaac tgccccacaa atagcagcta ctggtacgtc tgtttttaac aatttatcga
     4381 ttatttgata taaatgatga ttttttatgt cccatgaatt gccaccgatc ataataaaaa
     4441 ggtcaacttt ttcaggtatt tgttcgatta agtaatcgat ttttgttttt aatcctccaa
     4501 tggaagtgac ctctgattgg atagaagcag ttttgttttg ccatttctca ttttggttta
     4561 gtgtgctagt aatataagca ccttcccaat cggcatattc ctctaataga aacgtaatcg
     4621 cagttttcat tttcaagctc tcctttacta tctataacaa ggacaacagt tttaacctac
     4681 agacattcat taaaattggg tagcgacagc gaaaacaaca attcttaagt tttagacaga
     4741 gtatacagat agtcaactta attaaatacg aactaaataa aaatgacagg taatcatttt
     4801 tgtttaccag cattataaca ttttgcatac atggatgaca aaagaaccaa ttcccacttg
     4861 cttgagtaca atattaaaag aaactggaag gcaaaaagta attatagaaa cgaaaaataa
     4921 atcatatcgg gcactcagac atgaaaacaa ccctagagat atataactag gtaaccagtt
     4981 taatgaaaga aaaaatcatc caagaagtcg attcttggat tttttagtgg atttgttcgc
     5041 caaatacgtt tagtatgcgt atatgagagg ttgtaagcaa agcgttgtgg tctgagcact
     5101 taaggaggaa tctgtgaaaa tagcttttca tatttttgca gattttgact taatgccgaa
     5161 ggtcagcttt gcgattaccg tttatcgaga ggctgtaaaa gcaccgtgta gctccgatga
     5221 caataagcaa gcatttttag agctaggttt ccttgattct aaatatgcgg cttatttcaa
     5281 tgagttggtg cttgaacgcg attttaagaa ggtcgaaaac aggcacgact tttcgacgta
     5341 aggagaaaga atttcaatga tagaaacaat aaatcccgca gaaaacaagg tttctgcgta
     5401 cattcatatc ccattttgcg agcatatctg ctattactgt gattttaata aagttttttt
     5461 agaagggcag ccagtagatg agtatgtgga aatgctgctg aatgaaatga aaataatgct
     5521 agaaagtcat ccgacagcga atttagaaac actttacgta gggggcggga caccaacttc
     5581 tttatctgct aaacaacttg atcgactgtt atcaggtgct agagagatcc tgccttttca
     5641 aaatggtaat gagtttactg tagaagcgaa tccaggtgat ttgacccgtg aaaaacttca
     5701 agtaatgaaa aactatggcg tcaatcgttt atccatggga gtgcagacat ttgataatcg
     5761 cttattgaaa aaaattggac gtaaacacac cgcagaagat gtgtatcaaa ctatgcaatt
     5821 tttagaagca gaaaatttta ccaatgtaag catcgatttg atttatgcat taccgggaca
     5881 aacactagaa ggatatctgg atactttgga aaaagctttg gctttggatc tgcctcacta
     5941 ttctttgtac tcattgatcc tcgaaaacaa aacaatgttc atgaactggg tacgacaagg
     6001 aagactcgaa ctgcctgatc aggaaacgga aaccagaatg tttgaagaga cgattgaagc
     6061 aatggaaaag catggacgtc accaatatga aataagtaac tttgggctct ccggacatga
     6121 atctcaacat aacctcatgt actggaacaa tgaccactat tttgggtttg gagctggtgc
     6181 tagcggttat cttggagata ttcgttaccg caacaaagga ccaatccaac actatttacg
     6241 cccattacga gcaaatgaac gcccggtatt ggaagaagaa aaattgacac gaaaaaatca
     6301 aatagaagaa gaaatgttct taggactaag aaaaaaaaca gggattacta aaaaacattt
     6361 ctatgatcgc tatcaaaaaa caattgaatc ggtgtacggt gatgtgctaa gggaattaga
     6421 gcaagaaggg ttacttgtta atgaagaaga ccgtgtttat ttgacaccaa gagggacctt
     6481 tttggggaac aatgtgtttg agcgcttttt gtttgaaaaa tagtttaaag aatatttgtt
     6541 ttttatctga atttaaataa aaaaagttca aaaatacaat aaaggactat tgaaacgttg
     6601 atataactgg atttacatca attatttaga attagcactc ttgtgtttag agtgctaaaa
     6661 aatggagtct ttctcttgac aggagaaggg cttcttgcta tatttataag tggattagca
     6721 cttagaatat atgagtgcta atgagaggtg aggaagatgt taacaaagcg acaaaacgac
     6781 attttacgtc tgatcatcca acactatacg agtagtggcg tgcccgttgg ttccaaaaca
     6841 ttgatgtctg agggagtcaa ggccagctca gcaacgattc gcaatgatat gaaagcatta
     6901 gaagatgaag gattgctgtt gaagactcac tcttcttctg ggcggattcc atctgcttta
     6961 ggctatcgtt attacgtgga tcatttgtta aaacctgctc gcgtagcaga agatgatttg
     7021 ctgctgattc gtcagtctct aggcagagaa tttcatgaaa tcaatgagat tattaagcaa
     7081 tctgctgaga tattatctga gctgacaagc tatacgactt tttcattggg acctgaaata
     7141 aaagaccgcc gactgactgg ctttcgtatc gtacccttga ataatcgaca gattattgct
     7201 atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt tcactcttcc cgaatatatg
     7261 ggaagtcagg aactagaaaa aatggtacag attgtaaacg accgtctagt cggagatcct
     7321 ctgatgacgg tgtaccataa acttcgtacg gagattccga tgattcttca taaatatttc
     7381 caaacgacag aagggatgtc tcagttattt gacaccgttc ttggtcatgt ttttgaagat
     7441 aaggtttatg tcagtggaca aatgaatctc ttggattatg agccccgtca agatcttgat
     7501 cagttcaaat cgatgttttc ttttatgaaa gactcagacg gacttacaca aatgatcact
     7561 ccgatggata gcaatattca tatcaagatt ggtgctgaat taggcaatga tttattgcag
     7621 aatatgagta tgattcaagc aagttacgag atagatggtc atggaagagg tacaatagcc
     7681 ttgttagggc ctactagtat gccttattct cggatgttcg gtctagtcga tgtttatcga
     7741 agagaactag cagctaaact tgctgattac taccgttcgc ttgacttgtc aaactcgtaa
     7801 tgtgaataac gaagctattt gcagctgatc gttctctctt tcttagtaag agcaatcaaa
     7861 cagctgactg aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga agaactagat
     7921 aaagaaatga cagatgctca gccagaaccg gaaatcgatg tcgaagctgc agaagattcg
     7981 ggtatttcag aagctgaagc ggaagaattc gaaacagcga aattaaaagc tgagttagaa
     8041 gaaatggaag ataaatactt gcgggctcgt gctgaaatcg caaatatggc aaatcgagga
     8101 aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag atttagccaa aaaattactt
     8161 ccatcaattg acaacctgga acgtgcatta gccacagaag tgtcagatga tcaaggggca
     8221 ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact ggaagaagaa
     8281 ggaattgaaa aaatccctgc taaaggagaa gcctttgatc ctaatcttca tcaagcagta
     8341 caaactgttc cagcgactga agatacacca gccgatacga ttgtagaagt actacaagaa
     8401 ggctacaaat tacatgatcg cgtgttaaga ccaaccatgg tcattgttgc acaataattg
     8461 tgcggatata aaacttacac tttcaattaa agaaggagac gttaattatg agtaaaatta
     8521 tcggtattga cttaggtacg actaactctg cagtagcagt attggaagga aacgaagcaa
     8581 aaatcatcgc taacccagaa ggaaaccgta caacaccttc tgttgtatca ttcaaaaatg
     8641 gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca aatacgattt
     8701 catcaatcaa acgccatatc ggtgaagcag gttacaaagt agatgtagaa ggtaaatcat
     8761 atacaccaca agaaatctca gcaatgatcc ttcaatatat caaaggattc gcagaagact
     8821 atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt aatgatgcac
     8881 aacgtcaagc aacaaaagat gctggtaaga tcgctggtct agaagtagaa cgtatcgtca
     8941 atgaaccaac tgcagctgct cttgcctatg gtttagataa aacagatcgc gacgaaaaaa
     9001 tcttagtatt cgaccttggt ggcggtactt ttgacgtatc tatccttgaa ctaggagacg
     9061 gtgtctttga tgtattgtca acagctggcg acaaccatct aggtggggac gactttgata
     9121 acaaaatcat cgactacatg gtagcagaat tcaaaaaaga aaacagcatt gacttgtctc
     9181 aagacaaaat ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa aaagatttat
     9241 ctggcgtatc aagcacacaa atcagcttgc catttatcac agcaggagaa tctggaccat
     9301 tgcatttgga aatgacattg acacgtgcaa aatttgatga attaacagct gacttagtag
     9361 aacgtacaaa agtaccagta cgtcaagcgc taaaagatgc aggtttatca caatcagaaa
     9421 tcgacgaagt tattttagtc ggtggatcta ctcgtatccc tgcagttgta gaagcagtac
     9481 gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta gttgctatgg
     9541 gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt ttacttgacg
     9601 ttacaccatt gtcattaggt atcgaaacaa tgggtggagt gttcactaaa ttgattgacc
     9661 gcaatacaac gattccaaca agtaaatcac aagtattctc aactgcggca gataatcaac
     9721 cagcagtaga catccatgta ttacaaggtg aacgcccaat ggctgctgac aacaaaactc
     9781 taggaagatt ccaattgaca gatattccag cagctcctcg tggtattcca caaatcgaag
     9841 taacattcga tattgataaa aacgggattg taaatgtatc tgcgaaagat ctaggtacac
     9901 aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct aacagacgaa gaaatcgaac
     9961 gtatggtaaa agatgcagaa gcaaatgcag aagcagataa agctcgtaaa gaggaagttg
    10021 acctacgtaa tgatgtagat gcattgctat tctctgtgga caaaacattg aaagaattag
    10081 aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac agctcgcgat gaattgaaag
    10141 ccgctgttga agcaaacaac atcgaagaaa tgaaaactaa acgcgatgca ttgaacgaaa
    10201 tcgttcaaaa cttgactgtt aaactatacg aacaagcggc acaacaacaa gctcaagaaa
    10261 acccagaagc agctcaaggc ggagcagatg atgtcgttga tgctgatttt gaagaagtcg
    10321 atggcgacga taaataatca gttcactgaa tttcgagaga aggccaaagt tgctactttg
    10381 gctttttctt ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa aatatgatat
    10441 gataacaagc gacgtgaaaa tgatgagaac caataggagg aaagcctatg gcaacaaaac
    10501 gtgattatta tgaagtctta gggctctcaa aaggagcatc agaagatgaa ataaagaaag
    10561 cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa taaagaacct gatgcggaag
    10621 aaaaattcaa agaagtatca gaagcatatg agattttaag tgacccgcaa aaacgcgctg
    10681 catatgacca atacgggcat gcaggcacag atccaaatta cggtgctggt ggagctggtg
    10741 gcggatttgg tggattcggc ggcttctctg gtggcggctt cggtggtttt gaagatatct
    10801 tcgactcttt ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct cgtcaaggtg
    10861 ctgatttgca atatacggtc gacttgtctt ttgaagaagc gatttttggt gtagaaaaag
    10921 aaatcaagta taatcgggaa gaaatctgtc atacttgtgg tgggaacggt gcaaaaccag
    10981 gcacacaacc aactacttgt cataaatgtc atggtgcagg tacgattaat gtagaacgcc
    11041 aaacaccact tggacgagtg atgagccgtc aaacttgtga tgtttgtcat gggacaggga
    11101 aagaaatcaa agaaccttgt ccaacctgtc atggctcagg acatgagaag aaagcacatt
    11161 cagttaaagt gaatgtacca gctggtgtag aagaaggaca acaaatgcgt ttagctggtc
    11221 aaggagaagc tggagaaaac ggcggaccat ttggtgattt gtatgtggtc ttccgtgtag
    11281 aagaaagtga tatctttgat cgtgaaggtt ctgaaattta ctatgaattg cctttaagtt
    11341 ttgtacaagc agctttgggg gatgaagtcc aagtaccaac tgtccatgga gatgtgaaat
    11401 tgaagatccc tgctggaaca cagacaggta cgaacttccg tttacgtgga aaaggcgctc
    11461 cgaagttacg tggtggtgca acaggcgacc aacatgtcaa agtaaaactg atcacaccga
    11521 aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt cgctgaagct ggcggcatca
    11581 aagttgaaga acagcaagaa gacggtttct tcgataagtt taaagatgct tttggcggga
    11641 aaaagcggaa gtagtttttc tagtaaaaac gctaagtcat taataattaa aaaccagacg
    11701 aacaatcata tgttcgcctg gtttttttgt agttgtcatg tttgtaccaa caaaaaagcc
    11761 agctttagaa tatgataatc gtcatagttt ctaggggtat aaccagtgag agaatggaac
    11821 ttgttcaatc gatattgcaa ggtgttttta tgaataaata attcttctga gcaatttttg
    11881 atgcttccgt tatttttact gaagcatgcc aaagtcttct tgaacaaatg tacttcttct
    11941 tctgtcaggc ttttcaaaat acgttttaca tataatgttc gttgatctga cgggattgct
    12001 ggtaaaacga tcccgaggtc taaatcttca aaaaagcgga tctcgttcac agattgatgg
    12061 aaagctatcc attcgcaaac ttgtttggca tgaaggaaag attgcggtgt ctcttgctct
    12121 gtataacaaa gcgacccaat tccaaagttt agctgtaact gatacatttt attaaacttt
    12181 gtctggattt tatcaagcaa tgatagcaat gaggctctgg aaatccgatt gactaggata
    12241 atgatttgtt gtctatgtac agtgagcaaa gcctgtccgt aataagagaa gaccctttcg
    12301 agcaactcca ataaaggatt gatccgttct tgacacaatt gctgattgtc agaaagttgt
    12361 cccacaacta cataatatgg agaagaaaaa tcaagctcaa ttaagtgagg gtggtatcga
    12421 ctatgtactt gatatcatca atgaaccgat ggtactttct tatgcgatgg aacaggtaga
    12481 gcgattgctt gtttgtgcag gaaaacaagc gatgcggctg atacgattag gtgagaaaat
    12541 aagaagataa aaatacacgg tcccttcatg gcgaccgtgc atttttggtt tttattaaat
    12601 atttaatact ttattcaaga aatcttttgt ccgttcattt tgcggatttt caaagatttg
    12661 ttgaggcgtt ccatcttcta aaaagtttcc gtcgtcaatg aacatgacgc gattggccac
    12721 ttctttagcg aaccccattt cgtgcgtaac gataaccatc gtcatacctt gttctgctaa
    12781 ctttttcatg acgttcaaga catcaccgac catttcagga tcgagtgcag aagtaggctc
    12841 atcaaaaagc atgatgtcag ggttcatcgc tagagcacga gcaatagcca cacgctgttt
    12901 ttgtccacca gaaagcattt caggatacat atctttttta tcttctagac caactgtctg
    12961 cagtaatttg attgcttttt tctctgcttc ttctctagat tctttcttca ggtccatcgg
    13021 tgctaaaatg atgttttcca aaatagaaag atgagggaat aggttaaagt gttggaatac
    13081 cattccgata tgttgtctca ccaagttgat atctgtgctt ttatccatca agtgggcgcc
    13141 gtcaataata atttcaccgt cagaaggttc ttctaaacga ttcaagcaac gcaagaatgt
    13201 actttttcct gaacctgaag ggccaatgat acagacgaca tctccttcgt tgatggatac
    13261 attgatgtcg tttaatactg tgttatcgcc gtattttttg actaaatgct caactaagat
    13321 tttttctgcc atcttacttc accttctttt ctaaaacttt tgctaatttt gtcaatgctg
    13381 tgatcaaaat caagtacatg atcgcaatga tcaagtaaac atacgtactt tgtgttgtac
    13441 gtgcaacaat gatcttacca gtttgcagca attcgactac accgattgcg gaaatgatcg
    13501 ttgtgtcttt taatgagata acgaactgat tgacgaatga tgggatcatg atgcgtatcg
    13561 cttgcggcaa aatgattttt tgcattgtcc gattataaga aagacctaaa cttctggaag
    13621 cttccatttg tccgacagga acggcgttga tcccgcctcg aacaatctct gcgatataag
    13681 cacttgcgtt aagagtcaag gtgataacac cagcaaggaa gtcaggaatc gtaaatccaa
    13741 cgataccagg caacccaaag aaaataaaga acgcaagaac catcattgga attcctcgga
    13801 tgatatcgac ataaatacta gcaattgtgc gaagagcgcg tattggtgct acactgaata
    13861 atccaaaaat cacaccgatt actatagcca aagcaaagga gatcaaagtt aggagaattg
    13921 ttctccaaag tcctttcaac aatgcttgat agttgttttg gataagtcca agaaatgttg
    13981 attcatcagc agtggaagac tcactggaag tttctgaacc atccgctaca tattctttaa
    14041 taatcttatc gtattcgcct gttcttttta attctttcaa tccttcattg aacatattaa
    14101 gaagctcagg attttgtcct tttttaacgg caaatgcata ttttccgcca gattcttttt
    14161 tgatcggtgt ttccaattct tgtccttgtt tgatcgcata accgattacc ggataatcat
    14221 ccatcattgc atcgattgcg ccaactttta acccgtcgta caattgatca gcagcatcaa
    14281 aatatttgat agaatagcca tattcagatt catggtcgct taaaaattca gcactttcgg
    14341 taccaacttt tgctccgact gtttttcctt tcaaatcttc ataagacttg atcgaagtat
    14401 ctcctttttt gacagctaat tggatcccgc tatcaaagta agaatcagaa aaatcatagc
    14461 ttttctttcg ttcatctgtt acagtcattc cggcaatcat tccgtctgct tgtcctgatt
    14521 ccaaagcttg caaagcagca gagaaaccac ggtagtccat tcggatgtta aatccttgca
    14581 gtttggcgat gttttccatc aattttacat cgatgccttc gtactcgccc tttgcgtttt
    14641 gaaattcaaa tggagcaaaa gcagagtcac tggcaattac gtatacgtct tttttgggct
    14701 ctattttttg cattgtctcg cttgtgcttg actctgccgt tgtagtgttg tcagcttgtg
    14761 caacatgacc aaacgcaagt gtcacaagtc ctgtcattaa gaaaacaaat gagtgaaata
    14821 aatgctttct tttcataatc aacctcctaa atttccaata ttttattatt ataggcgaaa
    14881 agtgctgaat cagacaagac tgtaagctcg aaaaaatgaa aaaaatgcga gtttctaaca
    14941 ttttatgtta taaaacataa catgtataat aagataaaaa agtggcgaaa gtatgttcgg
    15001 tgtggtataa ttttttagta aggaatgcct ttgctttcct tttcttttta ttggtcgtaa
    15061 aatgaaaagg aatgaatgaa aaggaggacg aagaatgatt gagagagata cctcccgaca
    15121 ctttgagctt gtctcgaaat accaaccggc tggtgatcag cctgaagcga ttaaccaatt
    15181 agtggatggc gtagtcggcg ggaaaaaagc acaaatctta ttaggtgcga ctggaacagg
    15241 gaaaacatat acgatttcca atcttattga gaaagtgaat aaaccaacac tgattattgc
    15301 ccataacaaa acattggctg gtcagctgta cggggaattc aaggaatttt tccctaacaa
    15361 tgctgttgaa tattttgttt cttattatga ttattatcag cctgaagctt atgtgccttc
    15421 aagcgatacg tatatcgaaa aagattcaag tgtaaatgat gagatcgaca aattacgaca
    15481 ttcggctact agttcattat tagaaagaaa tgatgtgatc gtcattgcat ccgtttcatg
    15541 tatttttgga ttaggctcgc cttttgagta ccaaaaacaa gtagtttcga ttcgacaggg
    15601 agcagaacta gatcgcaatc agttgattcg tgacttagtc agtatccaat tcgaaagaaa
    15661 tgatatagat tttcagcgcg gtcgttttcg agtgcgcggt gatgtcgtgg agatttttcc
    15721 ggcttcaaga gatgaacgtg ccttacgtgt ggagtttttt ggagatgagg tcgaacgtat
    15781 ccgagaagtc aatgcactga caggtgaagt tttaggagaa acggaacacg tagcaatctt
    15841 cccggcgact cactttgtga cgaatgatga acatatggag catgccgtag cgaatatcaa
    15901 agcggaatta gaacagcgtt taactgtctt gcgaaatgaa aacaaacttc tggaagctca
    15961 acgtttagaa cagcgaacca attacgatat tgaaatgatg ttagaaatgg gctacacgtc
    16021 gggaattgaa aattattctc ggcatatgga cggacgtaaa gaaggtgaac cgccttatac
    16081 gctattggat ttctttcctg aagatttttt gatcgtggcg gacgaatcgc acgtaaccat
    16141 gcctcaaatc agaggaatgt ataatggtga ccgagcgaga aaacagatgc tagtagatta
    16201 cggtttccgt ttgccatctg ctttagacaa tcggccactt cggttagaag agtttgaaaa
    16261 acatgtcaat cagatcattt atgtctctgc gacgccagga ccatatgaac atgaacaaac
    16321 agataccgtt atccaacaaa tcatccgtcc gacagggctg cttgacccag tgatcgaagt
    16381 tcgtccaatc atgggacaaa tcgatgatct agtaggcgaa ataaatgaac gagtcgaaaa
    16441 agaccagcgg gtgtttgtaa cgacattgac gaaaaaaatg gcagaagacc tcacagatta
    16501 tttcaaagaa ttagggatca aagtcaaata tttgcatagt gatatcaaaa cgctggaacg
    16561 aacagaaatc attcgagatc ttcgattagg tgaatttgat gtgttagttg gaatcaattt
    16621 gcttcgtgaa ggattagacg tacctgaagt ttcccttgtt gctatcttgg atgcggataa
    16681 agaaggcttt ttacgtagtg aacgttcctt agtccaaacg attggacgag ctgctcggaa
    16741 tgaagaaggt aaagtaatca tgtacgcaga caaagtaaca gattctatgc gtctagcgat
    16801 ggatgaaacg tcaagacgtc gtacgattca gcagaagtac aatgaagaac atgggatcgt
    16861 tccaaaaacg atcattaaag aaatccgaga tttgatttct attacgaaag aatcggaaga
    16921 tgatacaaaa gaagcggtac aagtctcgta cgaagaaatg acgaaggaag aaaaagacac
    16981 gttattgatg aaattagaaa aagaaatgaa agatgcggca aaagctttgg attttgaaac
    17041 agccgcgaat gtgcgagata tgattttaga attaaaagct tcaaagtagg aggacaacat
    17101 ggctaacgat aaaattacga ttcatggtgc acgtgcccat aacttgaaaa atatagatgt
    17161 aacgattcca cgagataaat tcgtggtcgt tacaggtttg tccggttctg gaaaaagttc
    17221 cctagcattt gatacgctgt acgctgaagg gcagcggaga tacgtagaga gcctttctgc
    17281 ttatgctcgt cagtttttag gacaaatgga taagccggac gtagatagca tcgacggatt
    17341 gagtccggcg atttctattg atcagaaaac aacaagtaaa aatcctcggt cgacagtagg
    17401 tactgtgacg gaaatcaatg actatttacg cttgttgttt gcacgtgtag gacatccgat
    17461 ttgtcccaac gatcatatcg agattaccag tcaatcagta gatcaaatgg cagatcaagt
    17521 aatggctttg cctgagcgca caaagatcca agtgttggca ccgatcgtgg taaagaaaaa
    17581 aggacaacat aaaaaaatat tcgaacgaat ccaaaaagag ggatacgtcc gcgttcgagt
    17641 cgatggtgag atctatgatt taagtgaagt gcctgagctg gagaaaaaca aaaaacatga
    17701 tatcgcgatc gtcatcgacc ggatcattgt gaaagaaggg attcgttctc gcctatttga
    17761 ttctttagag gcggccctgc gattagcaga cggctatgct ctggttgacg tgatcggtga
    17821 agaagaaatg ttgtttagcg aacactatgc ctgtccttat tgcggattta ctgttggcga
    17881 actagaacca agactgttct cattcaatgc gccttttgga gcttgtccag actgtgatgg
    17941 tttaggtgtc aaattagaag tcgatcaaga tttagtgatt ccagatagaa cgaagacact
    18001 tagtcaaggt gctatcgttc catggaatcc aatcagctct cagtattatc ctcaaatgtt
    18061 agcgcaggct tgtgaaagtt tcggtatcga tttagataca ccattcgaag aattaccgga
    18121 agagcatcag gagatcatct tgaatgggac agatgatctt ttccatttcc attatgaaaa
    18181 tgattttggt ggcgttcgcg atgtagaaac gactttcgaa ggggtattga agaatatcga
    18241 gcgtcgttat catgatacga acagtgattt cacgcgtgag caaatgcgtc tttatatgac
    18301 agaattaact tgtcagacat gtaaaggtta tcgattgaat ccccaagctc tatctgttca
    18361 aatcaatgga acgaatatcg ggcaaacaaa tgagttgtct attcaaaaag cagtagattt
    18421 cttctcaaac ttaacgttat ctgaacaaga gaaagtaatt gccaaaccga ttttaaaaga
    18481 agtaaatgac agactttcat tcttggaaaa tgtgggacta gactatctaa ctttgagtcg
    18541 cgcttccgga acattatctg ggggagaagc gcagcgaatc agattggcta cacaaattgg
    18601 atcgaatctt tctggagttt tgtatatttt agatgaacca tcgatcggat tgcatcaacg
    18661 cgacaacgat cgactgattg gctcattaaa gaaaatgcgt gatcttggca acaccctcgt
    18721 tgtagtcgaa catgatgaag atacgatgtg ggcagcagat tatttgattg acgtcggacc
    18781 tggggccgga gaacaaggcg gacaaatcgt ggctgcaggc actcctgaag aagtagctaa
    18841 tgatgaaaat tctttgacag ggaaatatct ttcagggaaa aaatcaatcc ctgtaccaaa
    18901 agaacgccgt aaaggtaatg ggaaagcaat caagatcact ggtgcttcag aaaacaatct
    18961 gaaaaatatt agtgttgaat ttccattagg agaatttgta gcagtcacag gtgtgtctgg
    19021 ttctggaaag agtacattgg tcaatagtat cttaaagaaa tcgttagcgc aaaaattaaa
    19081 taagaattct gctaagccag gtaaattcaa gacaatttcc ggctacgaaa gtatcgaaaa
    19141 gatcatcgat atcgatcaaa gcccaatcgg ccggacgccg agaagtaatc cagcgactta
    19201 tacaagtgta tttgatgata tccgtgggtt atttgctcaa acgaacgagg caaaaatgcg
    19261 gggttataag aaagggcgtt ttagtttcaa cgtaaaaggc ggtcgttgtg aagcttgtcg
    19321 cggggatgga attattaaga tcgaaatgca ctttttgcct gatgtctatg ttccttgtga
    19381 agtatgtcat ggtaaacgat ataattctga aacattagaa gtgcattaca aaggaaaaag
    19441 cattgctgat attttggaaa tgacagtaga ggatgctgta gaattcttca agcacattcc
    19501 aaagattcat cgcaaactgc aaacgattgt tgatgttggc ttaggttatg tgacgatggg
    19561 gcaaccagca acgacattgt ccggtggtga ggcacaacgg atgaaacttg ccagtgaatt
    19621 gcacaaaatc tctaatggaa agaatttcta tatactagat gaaccaacga caggacttca
    19681 tagcgatgac atcgcccgct tgttgcatgt attacaaaga ttagtagatg ctggtaacac
    19741 agttttagtg attgaacaca atctagatgt aatcaaaaca gcagattata tcattgattt
    19801 aggaccagaa ggtggagaag gtggaggaac gatccttgcg actggaacac cagaagaaat
    19861 cattaacgta aaagaaagtt atacaggtca ctatttgaaa aaaataatgg tataaagagg
    19921 atagatagct gagatagtta aaaaaacata aaaaaatgaa tttttgcttg ttttattaat
    19981 atcagtgttc tagaatcatt acatggtagt tctttctaca ctgattggta tagataaaaa
    20041 cttcctataa gttcaatccg ttcaaaaaag aattgagcaa tcggaggact ttcctttgat
    20101 tgcttttttc tttatggtat gatattcctt gtgtagttgg aaaagaaaaa caagagatta
    20161 tgagtaagtg agagataatg atgaacagta ttgaaaatga agaatttatc cctatattat
    20221 taggaagcga catgaacgtt tatggtatgg cacgttcttt taatgaagca tacggcaaaa
    20281 tctgccaagc atatgcttcg gatcagttag cccctacccg ctacagtaaa attgtcaatg
    20341 tagaagttat tccaggattt gataaagacc ctgtatttat cgagacgatg cttcgtttgg
    20401 caaaagaaag atattctgat aagagtaaaa aatatctttt gattgcttgt ggtgatggat
    20461 atgcagaatt gatctcccaa cataaacaag agctgtcaga atattttatc tgtccatata
    20521 ttgattattc tttatttgaa aggctgatca ataaagtaag cttttatgaa gtttgtgaag
    20581 aatatgatct tccgtatcct aaaacattga tcgtcagaga agaaatgtta gtaaatggac
    20641 atttggaaca ggaattgcct tttgaattcc cagttgcttt gaaacctgca aacagtgtgg
    20701 agtatctttc tgtccaattt gaaggaagaa aaaaagcttt tattttagag actagagaag
    20761 agttcgattt aattttaggc cggatatacg aagccggtta taaaagtgaa atgattgtac
    20821 aagattttat cccgggtgat gatagtaata tgcgtgtgct aaatgcgtat gtagatgaag
    20881 atcatcaagt tcggatgatg tgccttggtc atccactatt agaagatcct acaccagcat
    20941 cgattggtaa ttacgttgtg atcatgccag attataacga aaaaatctat caaaccatta
    21001 aggcattttt ggaaaaaatc gaatatacag gctttgccaa tttcgatatg aagtatgatc
    21061 ctagagatgg agaatataaa ttatttgaaa tcaatttgag acaaggtcgt agcagtttct
    21121 ttgtgacatt gaacggcttg aatctggctc gttttgtcac agaagatcga gtgttcaaca
    21181 aaccctttgt tgaaacgacc tatggaacga atcaatcaga taaagcaaga ttatggatgg
    21241 gtgtgcccaa aaaaatcttt ttagaatatg ctcgggaaaa tgaagacaaa aaactagctg
    21301 agcaaatgat caaagaaaat cgttacggta ctactgtatt ttatgaaaaa gaccgatcaa
    21361 tcaagcgctg gttgttgatg aaatatatgt tccataacta tattccaaga ttcaaaaagt
    21421 attttcatgt gaaagaaggc tagttcatgg agaatttttt cagtatttta ggcggaatgg
    21481 gcacgatggc gacagaaagt tttgttcgct tgatcaacca ccgaacgaaa gctacaaaag
    21541 atcaagaata tttgaattat gtcttattca atcatgcaac agttcctgat cgtacagctt
    21601 atattttaga tcgatcagaa gaaaatccaa tgccgtttct gctggatgat attgagaaac
    21661 aaaatttatt gcgaccaaac ttcattgtgt tgacttgtaa caccgcacac tacttttttg
    21721 aagagttaca agcggcaacg gatattccta tcttgcatat gccaagagaa gcagcaaatg
    21781 aacttgtccg tcagcatacg acagggagag tagcgattct aggaacagaa ggaagtatga
    21841 aagctggaat ctatgaacgc gaagtcaaaa atcttggttt cgaaacagtg attcctgata
    21901 ctgcgcttca agaaaaaatc aattacctga tctatcatga gatcaaggaa tcggattatt
    21961 tgaatcaaga gctctattac gaaatcttag aggaagcggt agagcgccta aactgtgaaa
    22021 aagtcatcct aggttgcaca gaattatctt tgatgcatga atttgctgaa gataatcatt
    22081 acccagtgat cgatgctcaa tctattttag cggatcgaac gatcgaacgt gcattagctg
    22141 aaagaagcga ggcattggat acagcatcgg aaaagtaaaa aaattggatt tggggcaaat
    22201 atcctaaaag atatttggtc caaatctttt tgtaaaagaa aaagaaaact ggtaatgata
    22261 cgcccgggat gatataattt tattatgcac aatgtgagag gggttcgtaa atatgacaga
    22321 taatttgcaa ttagtcatca taacaggtat gagtggtgca gggaaaacag tagctatcca
    22381 aagttttgaa gatatgggct atttttgtat cgataatatg ccgcctcgat tgattccaaa
    22441 attctgggaa ttgatcaaag aatcaggtaa agtcacaaaa atggctttag gagtggattt
    22501 gcgttctcgt tccttctttg aagaaatcca aaacatgctg atcgaaatcg aaaatacgaa
    22561 cttcatcgac acttctatct tatttttaga tgcttcagac gaagaactag tggctcgcta
    22621 caaagaaaca agacgagcac atccaatggc gatggacggt ttgatcactg aagggatacg
    22681 taaagaacgg gcgatactag acgacttgaa agcccaagct tccgtgatca ttgatacgac
    22741 aacattgaca ccacgacaat tacgtgaaaa aatcaatgag tcattcaaat caaagggaga
    22801 ccaaggattt cggatcgaat ttgtatcatt tggttttaaa tacgggttgc cgatcgattc
    22861 ggatattgtg atggatgtgc gttttttgcc caaccctcat tatatcccgg aactaagacc
    22921 attgaccgga aaagatcagc cagtctatga ttatgtgatg tcctttgatg aaacgcaatc
    22981 attctaccat aaatttttgg atcttctgga aactgtattg cctggataca ttaaagaagg
    23041 aaaaagcagc ttgacagttg caatcggctg tacaggcggt caacaccgtt ctgtggcatt
    23101 gactgaacgt ttggcaaatg ctttagggaa aaactataaa gtcaatgtga cacatcggga
    23161 caaggataaa aggaaagaga cggtgaaccg gtcgtgaaaa tgaaaaccta ccggataaga
    23221 aaaccaaaga tcgtagtggt cggcggtggc actggtctgc cagttatcct gaaaagttta
    23281 cgaaatcaag gagcagatat cacagcagta gtgactgttg cagatgatgg tggaagcagc
    23341 ggtgcaatca gagaatcgat cgctatggct cctcctggtg acttgagaaa tgtcttagtg
    23401 gcgttatcgg atatgccaca attctatgaa gatattttcc aataccgttt caaaaaggaa
    23461 gatcagtttt tagccaatca tacgatcggt aatctgatca ttgccgcagt ttccgagatg
    23521 cggggaagca cttatgaggc gattcaatta ttagcaaaaa tgatgcatgt agatggacat
    23581 gtttatcctt cttcagaaac tcctttgacc ttgcatgctg tttttaaaga cggaacgact
    23641 gcagtaggag aatctaaaat cgcgatcaac cgtaaaacca ttgaccgtgt gtatgtctgc
    23701 aacacaaatg ataaaagtga agccaaagcg gctcgaaaag tcgtttcagc aattatggac
    23761 gccgacatgg tcgttttggg acccggaagt ttattcacca gtatccttcc aaatttagtg
    23821 ataccagaaa tcggcgaagc aatgctcaag acaactgctg aaaccgtcta tatctgtaat
    23881 atcatgaccc aaaaagggga aacggaacat ttcacagatg ccgatcatat acgtgtttta
    23941 cacaagcatt tgaatcagcc ttttatcgat acagtattgg tcaatacgga aaaagtgcct
    24001 gaagattata tggacccaga gatctatgat gagtatctag tacaagtcgc acacgacttc
    24061 aacggattga gaaatgaagg atgtcgagtg atttccaccg actttttaga attgaaagat
    24121 ggtggtgtct tccacgatgg agacaaagta gtagaagaat tgttccgttt agttttcggt
    24181 tcaaaatatt aagcaattta ttgttggaga aaggaggaga ataggatgtc atttgctgca
    24241 gatgtgaaga aagaactaac tggtttggaa gttcatcgag aacatgcgaa agcggaatta
    24301 gctgctttgc tgcgaatgaa cggctcttta ggtattgtga accagcagtt tattttgaac
    24361 gtccaaacag aaaatgctgc aattgccaga cgtatctatt ctcttttgaa agatcattat
    24421 caggtacgaa gtgagctact cgttcgtaaa aaaatgaaat taaaaaagaa caatgtctat
    24481 atcgtccggt tgaagcaagg cacaaaagat gtgttgatgg atcttgatat catggatggt
    24541 atgatgttca atggacacgt ttcggacgag attatgggaa attcgcaaaa aatgcgttct
    24601 tacttacgtg gtgcttttat ggcctcaggt tcagtcaata atccggaaac gagtcgttat
    24661 catttggaaa tattttctat ttatgaacag cataaccaag acatttgcga tatgttaaat
    24721 tattacggtc tacatgctcg aacgcttgaa cggcgcaatg gctatatttc gtatttaaaa
    24781 ggagcagaaa aaattgctga tttcttaaca ttgattggtg caaccaactc aatgctccga
    24841 tttgaagatg ttcgaatcgt acgggatatg agaaattcgg tcaatcgtct agtcaattgt
    24901 gagacagcaa atctgaacaa gacgattgat gcggcttcta aacaaatcga aaacatcgaa
    24961 ttgatcgaag caaaggtcgg tctacatgcc cttcctgaaa aacttcagga aatcgcagaa
    25021 cttcggcttc aacatccaga ggtcagcttg aaagaattag gggaaatgat tccctcaggt
    25081 gcaatatcaa aatcgggaat taatcaccga attcgcaaaa taaatgaatt tgcggaaaaa
    25141 ctgcgtgaag aagcagttta atccaaagaa aaaactgata catgaagcag gagatgcgaa
    25201 tgtatcagtt ttttttaaaa gctatacttg cttttcttcc gtcaaactat aattccaact
    25261 ttactggagt aacataatgt tgaaaggctt ctattgtttt ttaatacaat cgtataaaaa
    25321 gcggggagac aactgaatga agattttagt tttatacata aacttttgct taatatagaa
    25381 gtcctgatat agataggatc aagtatttat ctataactgg agattaatta aaaactggaa
    25441 ggaactgctc gatcaacctt gagaactgtt tttcttactt ctggtcaaga ttctcgtggt
    25501 tggtggataa ggatggttga taagttcatt ggattagcta gtcgaaaaca accaggtgta
    25561 attaatgtag aagttttaac aaggggatgg agtgatttat tcagtgataa tcataagttc
    25621 ataaatagta attgggatgt atggttggta tacgttatac ctgaacttga actatcaaga
    25681 tttggtttag aatttgagaa tcgatgttta taaagggagg gcaaaaaatg tgctttttta
    25741 caacttttac tatcattatg attgcagtac tttttatttt tctagatata gcaaagcgaa
    25801 atacagcatt tttattatat cgggttttac ttcgagcagg tctcataaca tttatatcga
    25861 tcgttggatt ttttctattc acagttattg tatttatttg gagaactaca gcaccaccat
    25921 tgccagagat tacatatgga gaatttccgt ttagtcttga atatgaaata gatgaggaaa
    25981 gaattttgat tgaagatacg gtgattgcag aatttatagg aagttttaga gggaatgtga
    26041 caactcaacc tagacgaagt tggagtataa aacttttaaa taatagtgga gagttgaaag
    26101 catttcatta tgaattctta ataatagaaa gtgaagatgt aacaatcact ttaagtagtg
    26161 gctctgctgg atattttatg aatgatggtg gaagcaagcc acagctcaca gtccgtgatc
    26221 gtattgcagg ttttatgcat ttttctcact atgacttaga agaagctcgt aaaatacttg
    26281 cagaatatgg gatcacatta atcaattggg aatatgcgcc accgataaga aattttttca
    26341 atgattatta ttagtcttat aaaaaacaga atgtaaaatt tatgtgcgcg agtatttagt
    26401 tttccatttt ttgtttgata gatatatagc attgttgaag catgggatag tggtgccaat
    26461 tttatcaatg aggttggtca ggcagttaac ataattggtg atgctataaa tccatttaat
    26521 tggaattgga ggaattaggt gtggaaacag tgagattatg gatattcatg aaaaattttc
    26581 gttcggcttt gagatatgtt attctggaat caaaatcagg ttattatctg attgacacag
    26641 atcactatac tttttgggga ggctaccttt ttccgatttt taattggata atcccccata
    26701 aggttatgaa aattaacttg cctgcagaag aaataaatag attaatactc aacaaagatt
    26761 tacaaaggaa aagagagaaa gaacattcta atgttgcttg gataggtcca gcatttgcag
    26821 tagtgacttc ttcaattgtc ggtcctatgc tagagagtat ttatagtgat ttttctattg
    26881 gaatacgtta tttatttgtt actttaagta tagctgttct tttgtgcata aagggttatg
    26941 ttagcaagaa gaataaagat attttaacac taatcggtgt aaacaattta tctagtacta
    27001 aaattatact gtttcctgaa tctataaaag aaggatcaag gttgttgata atttttataa
    27061 tgcttatact gttttctgtt ttatttgggg tgatgtttat ag
//
LOCUS       DAXLPI010000025        26726 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXLPI010000025 DAXLPI010000000
VERSION     DAXLPI010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.025"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(20..967)
                     /locus_tag="ACF3T5_000951"
     CDS             complement(20..967)
                     /locus_tag="ACF3T5_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631578.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(1096..1860)
                     /locus_tag="ACF3T5_000952"
     CDS             complement(1096..1860)
                     /locus_tag="ACF3T5_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD3631579.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(1864..2253)
                     /locus_tag="ACF3T5_000953"
     CDS             complement(1864..2253)
                     /locus_tag="ACF3T5_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631580.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            2388..2930
                     /locus_tag="ACF3T5_000954"
     CDS             2388..2930
                     /locus_tag="ACF3T5_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFD3631581.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            3078..3710
                     /locus_tag="ACF3T5_000955"
     CDS             3078..3710
                     /locus_tag="ACF3T5_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631582.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(3844..3969)
                     /locus_tag="ACF3T5_000956"
     CDS             complement(3844..3969)
                     /locus_tag="ACF3T5_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFD3631583.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(4103..4996)
                     /locus_tag="ACF3T5_000957"
     CDS             complement(4103..4996)
                     /locus_tag="ACF3T5_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFD3631584.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(5178..6104)
                     /locus_tag="ACF3T5_000958"
     CDS             complement(5178..6104)
                     /locus_tag="ACF3T5_000958"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFD3631585.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(6189..6950)
                     /gene="pflA"
                     /locus_tag="ACF3T5_000959"
     CDS             complement(6189..6950)
                     /gene="pflA"
                     /locus_tag="ACF3T5_000959"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFD3631586.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(7194..9425)
                     /gene="pflB"
                     /locus_tag="ACF3T5_000960"
     CDS             complement(7194..9425)
                     /gene="pflB"
                     /locus_tag="ACF3T5_000960"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFD3631587.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(9702..12152)
                     /gene="parC"
                     /locus_tag="ACF3T5_000961"
     CDS             complement(9702..12152)
                     /gene="parC"
                     /locus_tag="ACF3T5_000961"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFD3631588.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(12164..14209)
                     /gene="parE"
                     /locus_tag="ACF3T5_000962"
     CDS             complement(12164..14209)
                     /gene="parE"
                     /locus_tag="ACF3T5_000962"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFD3631589.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(14375..14809)
                     /locus_tag="ACF3T5_000963"
     CDS             complement(14375..14809)
                     /locus_tag="ACF3T5_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFD3631590.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            14936..15574
                     /gene="plsY"
                     /locus_tag="ACF3T5_000964"
     CDS             14936..15574
                     /gene="plsY"
                     /locus_tag="ACF3T5_000964"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFD3631591.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(15706..16581)
                     /locus_tag="ACF3T5_000965"
     CDS             complement(15706..16581)
                     /locus_tag="ACF3T5_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFD3631592.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(16663..17454)
                     /gene="codY"
                     /locus_tag="ACF3T5_000966"
     CDS             complement(16663..17454)
                     /gene="codY"
                     /locus_tag="ACF3T5_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFD3631593.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(17472..18872)
                     /gene="hslU"
                     /locus_tag="ACF3T5_000967"
     CDS             complement(17472..18872)
                     /gene="hslU"
                     /locus_tag="ACF3T5_000967"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFD3631594.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(18886..19434)
                     /gene="hslV"
                     /locus_tag="ACF3T5_000968"
     CDS             complement(18886..19434)
                     /gene="hslV"
                     /locus_tag="ACF3T5_000968"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFD3631595.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(19471..20376)
                     /gene="xerC"
                     /locus_tag="ACF3T5_000969"
     CDS             complement(19471..20376)
                     /gene="xerC"
                     /locus_tag="ACF3T5_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFD3631596.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(20574..21950)
                     /gene="trmFO"
                     /locus_tag="ACF3T5_000970"
     CDS             complement(20574..21950)
                     /gene="trmFO"
                     /locus_tag="ACF3T5_000970"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFD3631597.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(21919..23997)
                     /gene="topA"
                     /locus_tag="ACF3T5_000971"
     CDS             complement(21919..23997)
                     /gene="topA"
                     /locus_tag="ACF3T5_000971"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFD3631598.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHGHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(24119..24976)
                     /gene="dprA"
                     /locus_tag="ACF3T5_000972"
     CDS             complement(24119..24976)
                     /gene="dprA"
                     /locus_tag="ACF3T5_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFD3631599.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25032..25799)
                     /locus_tag="ACF3T5_000973"
     CDS             complement(25032..25799)
                     /locus_tag="ACF3T5_000973"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFD3631600.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(25792..26652)
                     /gene="ylqF"
                     /locus_tag="ACF3T5_000974"
     CDS             complement(25792..26652)
                     /gene="ylqF"
                     /locus_tag="ACF3T5_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFD3631601.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
BASE COUNT         7097 a         5735 c         4577 g         9317 t
ORIGIN      
        1 atatatatag taataagact taattcgttg tctctctgat aactagttca ggaaaaaaac
       61 gacgataacc ttgactcgcc cctttttctt ctattttttt taacaacatt tctccacata
      121 cttcacccat acgtataata tcttgcttga tcgttgtcaa tttaggtgaa ctgatttggt
      181 ctaaaaatac cccatcaaat ccaatcacac caaattcttc gggcaagtgt cctccagcat
      241 ttataatccc tcttactgct cctagtgcta gccgatctga actacataca aaacaagttt
      301 tatctgtaaa atctgcccag ttttctgtca tatatctttc tacttctgtc gaatgatttg
      361 gaaaccgcaa gattttacta ggaagatcaa cttccttcat cacagataaa taaccagttt
      421 cccttgaaat ttcaaaaggc tcgtccactt cgataccgat ataaataatc gtttgataat
      481 ctctttctaa ggcatattgc gttgcttttg ctgtaccata ttcattatcg ctgtctacat
      541 aatcatatcc ttcagaattt tccccaaaca agacaactgg tttttccaaa cggtcgatcc
      601 aatcgaagtc tttttttctg acacctgtga tgatgtatcc atcacaagaa ccgacgtcga
      661 aggcatcatt tgtaactaat tggagagaat aatgcgcttt accgatccgt ttagcgattc
      721 ccatcagcaa attcatgtaa tagggctcag ttgtgtcgat ttcttccagt ataaataatt
      781 tgatgatctg cgaccgattc gtgacaagtg cttttgcagc aatatttgga cggtaatcca
      841 gttcagccat tgctgagaaa actaactctt tcaattcgtc agtcacctgt tccggatgat
      901 tgatgactcg agagaccgtc atttttgaaa cattggcacg ttttgcaaca tctgctaaag
      961 tgggcatgct ctcttccttt ctaacatgtt gtcattctta ctatcaatta taccaaattt
     1021 tcctaacaga gtttattata gcccaaaata aaaaacggaa aatcctctat cgtcatgagt
     1081 gattttccgt ttcaattatt tatacttttt tatcaaattt tttgctaaaa aagtggaagc
     1141 ttcttccatt agtggtattt gtaccatatg tcgctgtgtt tttgaggaac gaaaaacaat
     1201 ttcttttccg taagacttat gccgattttt ttctacaaat cgttccgttt gtatataagg
     1261 gattttttcg tcagcttgtc catgccaaaa gaacagagga cgtccagcta acttttccgg
     1321 ctgcaatgac aaatcatagc tcttaaccca tgaaatcaaa tcaaaatagt cttttggtac
     1381 gacataatta tattccgatg cgcgccttac gatttctttt ccatattcca aaggtgcagg
     1441 ggaacccatc aaacacacgc ccgctactat atgaggatgt ttagccaaca aggcgcatgt
     1501 tgtcattccc cccatcgaaa ccccgccaac tgcaagctga tcatgtagaa gacctctttt
     1561 ttctaaatgc ttgataagtg tgtcaaattc aaacagattt ccgtagatac tatcccaaaa
     1621 tgtataggat ggaatagaag aaaccggctg tttgcgctcc ccatgattca tcgcatctgg
     1681 caggatcaca cgaatgccct gctgcgcaat ttttcttcct tgcgtcaaaa ctaactcctt
     1741 acttgtctgc catccgtgat aatagatgac aacgggaata cgttcctctc ttaattcttt
     1801 cagaacaact tctaacactg gaatatgatg gacagaacgt ttcaatataa ttggattcaa
     1861 ttgttactca cctattttca ttaaatttcc tgatcgccat agcaaccatg ctccaacagt
     1921 tgtctggaac aggttcagtg taccaaataa tataaatgca ggactgaatg gctcgggaaa
     1981 taagcacaga gaacaaagtc ccaccactcc taatgctagc gaaaggagtg gataaataga
     2041 aaaattcaaa aaactggcaa cgctaccgaa aatattacca ataaacagta tcaaaaagaa
     2101 aaaaacgctt aaaattaaac catctgtcag cgacatctga aaaacaatgt tctctacctc
     2161 aattggctgt tgaatcgaat agacacctaa gtatatactg agaaggagac ttagcacgtg
     2221 gctggcacaa aacacacgat tgaccgtttt cattttaatc acctaaccta tctttataat
     2281 tctattataa tcattttatc atttttttgt atgtataaag tttcaatttt tcttcactta
     2341 cgattttttt tgatattgtt gttatataaa tgaaaggagt tttacaaatg attgatccaa
     2401 aaatgttggg gatgatcttc ctcattaatt ttgcttatat cactctgaac acgttacgtt
     2461 tcatgttgac aatgaaaggc tatcgactga ttgctccact tgtcagtatg gtcgagatca
     2521 cgatttatat cgtcggctta tcccttgttt tagatcgttt agataatcca ctgaatttat
     2581 ttgtctatgc gttaggttac gcagtaggta tcagtgtcgg aattttgatt gaagacaaat
     2641 tggcattagg atacatcatg gtcacaacga ttttgccagc aaatagcgca gaagataaaa
     2701 gtttgccggg tatactgaga gaacacggtt atggtgtgac acagacttct gcaatggggc
     2761 tggaaggtga gcggctagtt ctcgaaatac tttctcctag aaaaagcgaa cgtgaactgt
     2821 atcgcctgat caaagaaatc gaagagcgtg cttttatcat ttcttatgag ccaaaatata
     2881 tttcaggtgg tttctggact aaaaaagtga gaaaacgaca aggaaaataa ctcacaaact
     2941 taaaaactgt ctaatatcta aattaaattt taaatgttag acagttttta tttattattt
     3001 tgattaaaaa acatcgacat atactcatca atttgttatt atactttata tacttatcaa
     3061 tttaatttgg agatattatg gaaattcaac gtagaaccac taatttttta catttaggct
     3121 ctttttttgc aactttgacg atcttatttt ttctatgtta ttcatacctt aagacctcac
     3181 atgcgccttc tagtgcatta ggctggttgc ttttggatca ttctgatatc acagaagccg
     3241 aagaaaatcc gttttttttg attttgtctt ctctttgcgg cttactgttt tctgtttatt
     3301 tcggtgccta tttttttcat cgacatcctg gtacgttaaa cgaagcttca agcgtattga
     3361 ttgcatttaa aacgttttct tttttagcaa ttctttttta ttcgtttaat cacaatacgc
     3421 ttttcttctt tatcatgaat ggtttagtcg tgattttatc catttttact tattttattt
     3481 tttcactcat ttttagccaa ttgaaatgtc cgctcttctt tcgattagtt cctgtcacct
     3541 gttttattta cagcttgttt tccttttttg tccttgcttt aatgccactg accaaccctt
     3601 cgacgatcca agtcgcagga aatctgttgg atatgctctt catcgtcagt ctaggtgtat
     3661 ttatgtggca aagaaaaaac aaacaaaata aaaagcattt tgaagcctga ctattacgac
     3721 ttcataaatg cttttttagt tttaaacact tcaatctaaa agaataaccc aagattggat
     3781 aaattattaa tgactcgcca ttcgtttctt agtgattctg gattatcaaa tggacttcct
     3841 aatctaatca gctagttctt cccataaaat gccatacttg actgaatcat aatattgatt
     3901 atttaaaaat ctaacttttc ttatcactac ttcctttgtc attccacatt tctcaccatt
     3961 tttttgcatt cctttgtttc cagaccatgt agtgaatcta ataggtggta gataggcaaa
     4021 atactcaaat aattctttta accataaagg aagagaaagg ctcctcctgc aagagtcagt
     4081 ataatttttg cttactgctc tcttaggttt tactcactaa aactgttcca tattctttga
     4141 tataagaaaa tgcatgcggt ataaatttct tgctcagctc agaaaagatt tgtcgtttaa
     4201 gaaaggccat ttcctccatt gtcaatgaag gatccaatgt tggtaattca atctcaaaat
     4261 tgtttttttc gacaataggt aattctaatg tgaatgattc ttcttccttt atagtattcg
     4321 tcacaagtag agactgatgg aaaatactag gccggcgatt tttatattta ttcttacgta
     4381 tcctgtcaac gataacttct atttgatcaa aaaagtacga ttcatttacg tctccaataa
     4441 gttcggctaa ttctttgaat gccttcgtta caggaagctg attcttctgt acaaaaaagt
     4501 aattagccaa agtaagcggg aagtgttcta tagaggaaag tgtgataaaa cggtacttct
     4561 catttcctgc agttgtcctc agcaattctg tctctcggct gtttcttact ccataaaaat
     4621 acgcttcttt ccgttcatct tcgaaacaat acgcccaaat aacttctccc atcgctaatt
     4681 tttgttcatc aacagagagt ctcttcaatt gttcagctat ggtttcagca gccgaagtgg
     4741 cttgttgcaa gtaatcttta ggatcaaata ttggaaagtt caaatgatat cttcgttctt
     4801 ccctcttgat taatcctgca tcgatcaata aatcaagaaa atgggaaaat ttattttgcg
     4861 aaaattcttt tttcaattct cttagaatcg tttcttgttc tcgatgttca tatagataat
     4921 cggtcaatga agaaaaaagc ggatcatcgg tcagtttttg ataaaagttt tccttatttt
     4981 ttgaataata gttcatatat gttctctttt ctatccgttt tattttactt aaaaggcttt
     5041 tacgtaaaaa ggaggaagaa caagagtcat aattgactgt tattctcctc catttcttcg
     5101 gcctttagta tgttctatgt ttctttttat atcaatcgct tatccattca ttttgaatgc
     5161 ttcatactac catttgatta gctaaataca gccgtcaatt gaggtacgac ttgtttttta
     5221 cgtgaaacga cacctttaag caaagcacgg ttgttggtca aagttgtttg gaaagcttct
     5281 tctacttttt cttttccttt accagcaaca agtaattctg aatcactgtc taagatgttt
     5341 gtgacaacta atacgaacag atcgtagcca tttgctttca tttcatcttc cattgctgct
     5401 tctagctctg cttgtcgatc catcacttca ttaaggtcca ctgtattgac ttgagcgata
     5461 cgaacgttgg aactgcccat tgggaaactt tttgcatcca aatcaagaag aaccgcagct
     5521 gatttatcac ttagatttgt tcctgctttc aacatttcaa gtccataagt gtttagatcg
     5581 acttcagcga ttgctgcaag ttcttttgca gctgcttcat cttcaggcgt acatgttggt
     5641 gatttgaaca acaatgtatc tgaaatgata gctgagagca tgattcctgc gatttcttta
     5701 ggaattgtta cttggttttc tttatatagt tttaaaataa tcgtacttgt acatccaact
     5761 ggttctgcac gatagtacaa tggattagct gtttcaaagt ttgcaatacg atggtggtct
     5821 actacagcta aaatatttac ttcgctgata tctgaaatac tttgttggaa ttcattatga
     5881 tccacaagca tgacatctgc attttctgct ttagctgact ctactactcg tggtgcagat
     5941 agctgaaagt ggtctaatgc gtattgtgtt tcttcattag gtgtacctag agcaactgct
     6001 tctgtttctt tacctaattc attttgcaag tgtgaaaacg caatggctgc cccgattgca
     6061 tctgtatctg gattttggtg accaaaaact aaaatttttg acatgtcatt cgctcctttg
     6121 tgatttctcc tatttaataa tagcaaaaaa acggaaagaa gcaatactta tcttctttcc
     6181 gatgcgacct atcgcgttaa atattttgta tactcttctg tatgaagcaa tcgtttagca
     6241 ttttcgacac gttcttttgt aggtggttcg atcccttcta atggatattt caaaccaagt
     6301 gtttcccact tgtattttcc catcgtatga taaggaagaa tctctactct gtctacattt
     6361 gacaaggttt gaataaagtt gtctagtcgg atcaaatatt catcataatc acttcgctca
     6421 ggaactagta catggcgaat ccaaaccggt ttgttgattt ttgacaaata ttgagccatt
     6481 tctaaaatat tttcattgtc ccaatgcgtt agttttttat gctcttcatt atcgatgtgt
     6541 ttgatgtcaa ataacagtaa atctgtatac ttcatcaatt cttgaaaacg tgaaaagaaa
     6601 ggttcttcat acgtaaaagg ttttccgcat gtatccaatg tagtatgcat cttccgctct
     6661 tttgcttttt taaatagttc gatcaaaaaa tcgatctgca atagaggttc tccaccacta
     6721 acagtaatac cacctttatc tccccagtaa ggtctgaagc gttctgcttt atctaaaagt
     6781 tcatctgctg tgtattcttt ccctccgcca atattccaag tatctggatt gtgacaaaat
     6841 tggcaacgca tgcggcatcc ttgcataaac acgacaaagc gcagtccagg tccgtctact
     6901 gaaccgaaag tttcgataga atgaacatag ccgattgttt tttcttccat ttaagttcac
     6961 atcttttctt tgatttcatt ttaagtacaa gttagctgtc gaagcgaagc tttctgaaca
     7021 aaatttttcg caattgccgc ttcgttatgt gtaaaattta cagggacgga acatacaagt
     7081 cctgcccctg taaatcgttt ctttcaaata agcatactga ttattcgatt ttgatgcgat
     7141 ggctattcaa cgtcctttcg acgctaagtc gaaaaacttt tttcacgaac tttttacatt
     7201 ctgtcgtgtg aagttctcat caagacatct aattgttgtt ctcttgtcaa gtcacggaat
     7261 ttaactgcat aaccagatac ccggatcgtc aagttcggat atttcattgg attttccatt
     7321 gcatcacgca ataaatcatt actgaataca ttgacattca aatgataagc tccttttgag
     7381 aagtatccat ctaagacatt acgcaagttt tcgattcttg tatcttgttc ttttccaaga
     7441 cctgaaggat tgattgtttg ggtatttgaa attccatcca atgcacagcc gtaattcatt
     7501 ttagctgttg agttcaggct agctaataac ccactttgtt ccgcattgta acttggattt
     7561 gctcctggcg caagtggttt tccagcttca cggccatcag gagtatttcc tgtattttta
     7621 ccgtaaacaa catttgaagt aatcgtcaat aatgaagttg ttggttttga gttgcggtaa
     7681 gttttatgac gtttgatttg tcccatgaag tattccaaga tccatgaaac taattcatct
     7741 gcacggtcat catcatttcc gtatttaggg aagtcgccat ccacttcaaa atcaactgct
     7801 attccttttt cattacggat tgcttttact tttgcatatt tgatcgctga caagctgtct
     7861 gccgcatgag agatcccagc aatacctgta gcaaatgtgc gttgcaagtc agaatccatc
     7921 aaagccagct gagctgcttc ataagaatat ttatcatgca tataatgaat gatgttcaac
     7981 gtattgacat atagttctgc taaccaatcc atcatattca tgaagttatg tttcacttct
     8041 tcaaagtcta gtatttctga tgtgattgga cgatattctg gtccaacttg ggcgccagtc
     8101 acttcatcga ctccgccatt gatggcatat aatagtgttt tagccaagtt agcacgagcg
     8161 ccaaagaatt gcatgtcttt tccgacaact gttcctgata cacagcaagc aatcgcacaa
     8221 tcatcagaac cccattgttc acgaagctga ttatcatttt cgaattgaat cgaagaactt
     8281 tcgatagcga ttctgctagc atatgttctg aatccttctg gaaggtgttc agaatacaaa
     8341 acagtcaagt ttggttctgg tgaaggtccc atatttgtta atgtatgaag cagacggaaa
     8401 tcattctttg taactaatga acgaccatct aatcccatac ctgcaatgga taaagtagcc
     8461 cagataggga atcctgagaa taattggtta tattcaggtg tacgcgcgaa tttgaccata
     8521 cgtaatttca ttactaagtg gtcgatcaat tcttgtgctt ctttttctgt gattactcca
     8581 ttatccaaat cacgttggat atagatatca aggaatgctg agatacgtcc gattgacatc
     8641 gcagcaccat tttgtgattt gattgctgct aaatatccga agtatagcca ttggacagct
     8701 tcttttgcat ttgcagctgg tttagaaata tcatagccat atttcgcagc catttctttc
     8761 atttgcgcta atgcacgata ttgttcagtg atttcttcac gtaaacgaat cacttcttca
     8821 ctcattgtat gataaccagt catatcatga tcttttttct tttgttccat caaatagtcg
     8881 attccataca atgccacacg acgatagtca ccgatgatac gtccgcgacc gtaagcgtct
     8941 ggaagccctg taatgatttt gtttttacga gcagcacgca tttcagcagt ataagcgtca
     9001 aatacacctt gattatgtgt tttgcgccag tcagtgaaaa tatgtgtcaa ttcatcgttt
     9061 ggtgtatagc cattgctttt tagcgcattg ttagccatat tgattccgcc gaacggcatg
     9121 aatgcttgtt tcaaaggttt gtctgtctgt agaccgacga ttttttccaa atctttatcc
     9181 aaatagcctg catcgtgtga agtaatagtt gatacgattt ctgtgtccat atcatagaca
     9241 ccgttacgct cgaattggat atcatttaac ttttgcaatt catcccataa tttttctgtt
     9301 gcttctgttg ggccttccaa aaagctgtca tcaccgcgat attcagtgta gttattttga
     9361 ataaagtctc tagtgtctac gccttctttc catttggtac ctttaaatcc attccattgc
     9421 tccattgtat ggacctccta aatttctgtt ttatgtaaca gtttttacgt gattagtata
     9481 acacagcacc ctatctttgc aagggttttc ttgtttttta tttaacaatt tttatgtttt
     9541 atttacatat atttaaatca acgttgacat accaacattt aagcattgtg aacactttca
     9601 tctttttttg caaacgtata aggatttatt aaaaatgaat gaaaaagaga cgccacctgt
     9661 attaatacag atggcgtctc ttcattaaaa tactacaaca gttacgactc gatgaaaatc
     9721 tcaggagcat cgataacttc gatgacttct ccaccttttt tctcatccat cgcaaatgat
     9781 ccattcgatg ttcgatcacc tatagggaaa tcagttgggt caatcgttaa ctgctgccct
     9841 ttttgattca tcaaggttat ttcttttgat cttccttccg acatatagat aatacggtgc
     9901 ggattttttt tcagctcacg catgaccgtc actccccgct tcgcacgtga agtttggctg
     9961 atttcttgag caagcatgcg tttaacagct cctcgctgag taacgattac gatcggcgta
    10021 tcaccttctg ctaacacaag cagcccgttg ataatcctat ctccatcttt caaattcata
    10081 gacttcactc cagctgcctt tgctccaaca acgggaacat ctgttaaagg atagcgcaaa
    10141 cccattcctt gatgcgttac taagaaaaca tctaagcctg gctgatcttc tttcagatac
    10201 acagcaatca gttcatcttc ttccgccttt agcttcatac agctgagcgg acggctctta
    10261 taggtgcgcc atggttcgaa ctctgtcatt cgtgtttgct tgatcaatcc attcttgctg
    10321 atgaatacaa atgttttctc tgcttgaatt ttttgatatg ggaaaacagc taaaatcgat
    10381 tcgtctacag acagattgga gattgtttga gaaatgtgct caccagcatc tttccatttc
    10441 aaatcgggta gttcatggac agggcggtag atcacatttg ctttatttgt gatcagaaga
    10501 acatggtcca atgtactcaa ttcacctgag tatagcaagt agtcgccttc tcgcatcccg
    10561 atttcttctg gtttggaagc actataagaa cgaaggcttg ttcgcttcac atagccttct
    10621 cgcgtcacac tgacaattac gtcttcttgt gccactaaga cttgcgtatc aattttgatt
    10681 tcttcgattt cgtcttcgat tgtcgttaga cgagcggaag aataattttt tttcacttct
    10741 cgcaattctt ttttcataac agagaaaagc tctttgtcat ttgaaaggat tttattcaac
    10801 tctgtgattt gcgcaatcaa gctttcggat tccttttgaa gctctgtgat atccgtgttc
    10861 gtcaaacggt ataattgcaa tgtgacaatt gcttctgctt gttcttctgt aaattgaaag
    10921 acgtcgacta aattcttttt ggcgtctttt ttgtctttac tttcacgaat cgttgcgatt
    10981 acttcatcca gaatcgataa agctttcatc aatccttcta cgatatgctg tcttttttga
    11041 gctttggcta aatcgaattg actacgtttt gttatgactt gctttcgatg ctcgatataa
    11101 cttcttaaaa tgtctttcag tccaacttga tgcggagtca tatggtcgat agcgaccata
    11161 ttgaaattgt aattgatctg tagctcagtg tttttgaaca aataattcaa aattccttga
    11221 gcattggcgt cttttttcaa ttcgacgaca atttgcagac ctgttctatc actttcatct
    11281 cgtacttcag ctattccatc gattttctta ttcaaacgga tctcatccat ttttttcacc
    11341 aaggtcgctt tatttacttc atatgggatt tctgtgatca cgatttgctg tttattaccc
    11401 ttcattgcct ctatctttgt tttcgaacga agaataactt ttcctcgtcc tgtttcataa
    11461 gcttttttga tttcatcttt tccttgcaag atccctcctg ttggaaagtc aggtccagga
    11521 atatattcca tcaatttttc aaggctcgct tgtggatgat cgatcatata gattgtgcca
    11581 tcgatcactt cagctaagtt atgagtcggg atttctgttg catatccagc agatatacct
    11641 gtcgatccat tgacgagaag gtttgggtat cgagctggaa ggactgttgg ttctttttct
    11701 gtatcatcaa aattccacac gagatcgact gtttctttct cgatatctgc tagcatctct
    11761 ccacttaact tggataatct ggcttcagta taacgcatcg cagcaggtgg atcaccgtcc
    11821 atactcccgt tattcccgtg catttcgatc agtacttccc gcaacttcca gtcttggctc
    11881 atgcggacca tcgcttcata aatactgatg tcaccatgag gatgataatt ccccatgata
    11941 tttccgacag attttgctga tttacggaat cctttgtcat acgtattgcc atctttgttc
    12001 atggcaaata atatccggcg ttgcactggc ttcaatccat cacggatatc aggcagtgca
    12061 cgttcttgga taatatattt ggaataacgt ccgaagcggt cacccatcac ttcttctaaa
    12121 gttaattctt gtacttcttg tcgattttcc atcaggtgac ctcctattct acttcaaata
    12181 actgattgtt ttcattttta tctgctcgtt cttcatctaa taaatcatta gaaactgaag
    12241 gagagatttc agtatcctct tttttatcta agatactgcc atcttcttcg agcgtaaatt
    12301 gcacgtgatt ttcgatccat tttcttcgag gttctacttt gtcacccatc agtgtggtta
    12361 ctcgacgttc agcttgcgca gcatcatcga tacggacgcg gatcaacgta cgggaagtcg
    12421 gatccatcgt tgtttcccac aattgttccg cattcatctc tccaagtcct ttatatcgct
    12481 gcagcatata tcctttgccg acttttttaa tcattgctgc caattcgtca tctgtccacg
    12541 catattcaat gacttgcttc tttccttgtc ctttggatac tttatataaa ggcggtaacg
    12601 cgatatatac tttcccagct tcaatcagcg gtttcatata acgataaaag aaagtaagca
    12661 gtaatacttg aatatgggcg ccgtctgtat ccgcatcggt catgatgatt actttgtcgt
    12721 aattacagtc ttcgatagaa aactcaggac cgactcctgc accaatcgta tagatcatcg
    12781 tattgatttc ttcatttttc aagatatctt gcattttagc tttttcggta ttgatgactt
    12841 ttcctcgtaa aggtagaatt gcttggaact ttctgtctct tccttgttta gcagatccgc
    12901 cagcagaatc cccttcgact aaatacaatt catttttctt agggtttcgg gattgtgcgg
    12961 gtgtcaattt tcctgaaaga agagattcgc cttttttacg cttcttccca tttcggcttt
    13021 cttctctggc tttacgtgct gcttctctcg cttctctggc tttgattgct ttacggacca
    13081 acatctggct catttcgcta ttttcctgca aataaaagcc catttgttca ctgatgacgt
    13141 tatctactac ggtacgagca actggcgtac caagtttttc ttttgtttgc ccttcaaatt
    13201 gcagaagatt ttcaggtaca cggatagaaa gtacagcagc taacccttct ctgaaatcac
    13261 taccttccag attcttgtcc cgttctttca gcaaaccaac ttttctcgca tattcattat
    13321 aagatttcgt catcgccgct ttcataccag cttcatgggt tcccccatct tttgtccgaa
    13381 cgttattgac aaaagataaa acattttctg aataaccgtc attgtattga taagcgacct
    13441 ctacttcgat accttctttt tctcctgaaa aataaacaac tggtgtcaat gtatcttttt
    13501 cttcattcag ataatcaaca aattctttga ttccttcttc gtaatgaaaa atttccttga
    13561 ctggttcttc tccacgaaga tcagaaagag agattttaac gccttttaga agaaaggcag
    13621 attctcttaa ccgttcggct aaaatttcat atgaaaattt cgttgtactg aatattgtat
    13681 catctggtaa aaaatggaca gtcgtcccgt ttgctttttt tgtttttcct atctttttca
    13741 aagtaccgtc aggtttaccg ccgtttttaa atttctgctg atactctacg ccatctctaa
    13801 cgatcgttac aattagccat ttcgataagg catttaccac actggctcct acaccgtgca
    13861 gtccaccaga agttttgtac cctccttgac caaactttcc gccagcatgc agcacagtaa
    13921 aaatcacttc aactgttggt attccggaag catgcattcc tacaggcatc ccacgtccgc
    13981 tatctgcaac agttatacta ttgtcttcat ggatggtcac atcaatctca ctgccataac
    14041 ctgataatgc ttcgtcaact gcattatcga cgatttcata gactaaatga tgcaagcctc
    14101 ggctgtcagt ggatccaatg tacatccctg gtctttttct aactgcctct aatccttcca
    14161 atacttggat ggacgagtca ttgtattcgt tgtttacttt ttttgccaag gcaaacgctc
    14221 cttcaataat actttgcggc aaaaccgcac cacgattatc atactagaaa aggtcgtgaa
    14281 aaaaaagagg gtagacgaga aatcaatttt aattatctcc taaccgcttt ttcgacattc
    14341 ccctctatcg atcatctctt ctttgtggtc atttttattt ttggttttct ttcaacttac
    14401 caagttcaat cttaatacaa cgatccatta cgatatctgt acgtcctgct tttcttagca
    14461 tctcagctgc ttctttgttt tcaatcccta attgtgccca gaacacttta gcatcagttt
    14521 tcaaaaaatc acctgctatt tcaggtaaaa attcactccg tctaaataca tctacgatat
    14581 cgattggatc atcgatttct gtcaaattcg catagacttt ctcgcctaga atttcttcac
    14641 cagccaataa tggatttact ggtatgattt tatatccatt gtcttgcaat acctgtgcaa
    14701 ccatgtaact tgttcgttct ggtttattgc tcaagcctac gacggcaata ttttttgctt
    14761 cttccaaata gttgaaaatc gtttcttgtg ttggattttt tacagacatt tctgccaccc
    14821 cttgcttttc tttttattcc atctattata cttgtatctt tcattttttc aattgttcct
    14881 gttacctatt catgctaaaa taaaggatag ttttcaactt gaaagagggt aaaaaatgaa
    14941 attgatctta ttacttattt tagcatatct tttaggttct attccttccg gtgtctggat
    15001 tggcaaattg tttttcaaaa aagatatccg acaacacggg agcggtaata ctggaacgac
    15061 caatacattt cgtgtattag gcaaaacagc cgggattgtt gtcctgctta tggatatttt
    15121 aaaaggtaca ttggctactt gtttgccgat gatcttccac ctttctggga ttaaccctct
    15181 ttggtttggg gtatgcgcca ttcttggcca tacatttccg atttttgctg ggttcaaagg
    15241 aggaaaagct gttgcgacaa gtgcaggaat gcttttagga tttaatcctg tattttttgt
    15301 ttattcttgt atcatcttta tcgttagttt attttgtaca agtatggtga gtttgagcag
    15361 tatgatcagt gcagtattga tcactttatc tacgatcgtc cttccttatg tagctcctgt
    15421 gattttggct aagcccaatt ggttactgac gatcattgcc tttttagttt cttcctttat
    15481 cttttatcgg cataaagaca atatcaagcg tatcagaaac catactgaaa gtcgaatccc
    15541 gtttggtttg aatagcagca agaaaaaatc ctgatacttt ttcattccaa agagataaaa
    15601 cgggagtgtg acaaatcgat gtcacactcc cttattactt acaggatagc ttttttacac
    15661 accttggtaa acggtgttcc ctgctaactt ctcggtcaca acctcttact tcactgtgat
    15721 ggaatatttt gtcttgaaga tttcatgggc acctagttga ttgatcccta ctttttccat
    15781 caagtttcca ctactgttca ccgtgtcagc gattccgcac cacggttcga tacaaacgaa
    15841 aggcgcttct tttggataag gagaccagat ccctacataa ggcatatctt tatagcttaa
    15901 agttacgcta tgggaagatt tttcacttcg aatggcgtac gcatttagac cccttgtttc
    15961 aaaaaccaag gcatcttgct caaaaagttc acgattcaaa gccagattcg tatttgtctg
    16021 acctaacgtc ttttgatcca tatcgatgaa tgggcctttc aatggtaggc gtaatcttga
    16081 tttcaatgga gaaaatgcca gatagaaatc ttcaaatgac aatccttctt caagaggtac
    16141 attgaaagca ggatgtccac cgatagaaaa gaacatttct ttttcaccgg tatttgccac
    16201 ttgataatga accgtgatgc cgtctcctcc taattcatag ctgatcacta attcaaaatc
    16261 aaatggatac ttttctaatg tctccggcgt actccttaag atgaaactag ccatttcttg
    16321 tccatgttca atgacttcaa aatccatatc ccgagcaaat ccgtgttgcc ccatttgata
    16381 tgttttccct tgataaacat actgatcatc ttttaaacgt ccaacaaaag ggaataaaac
    16441 cggtgcatgt ctgccccaat acgctggatc accttgccag atatactcta aaccatgttc
    16501 ttttgatttc agactgatca attccgcacc gtgttcagcg atttcggcga tcagctggtc
    16561 attttgaata ataacagtca ttgccattcc tccaacttcg acttactttt taaaatggga
    16621 aggctggaac aaacatttct atctgcccca gtccccttac ttctaataac tgtgttttgc
    16681 taactcgtct ttaaaacggg aattcaatac ttttaaataa gttcccttca tgcctaacga
    16741 acgagattca atgattccag ctgattccaa ttttcgtaaa gcattgacga tcacagaacg
    16801 agtgatgccg atactatcgg caatattaga agcagtcaga cgaccttcat ctccatccaa
    16861 tgcttcaaag atagcttgta cagccttcaa ttcgctataa gataatgtgt tgattgccat
    16921 ttggacagcc gttgcactcc gtacatctgc ttcgatgctt cgagacttct gataaaggat
    16981 ctgcataccg acaaccgtag ctccatattc tgctaagact agatcgtcat ctgcaaacga
    17041 ttgttccata cgagataaga taatcgtacc caaacgttcg ccagctccaa agatcggaac
    17101 gacagtcgtc agaccaaatg gatatttctc tcttgactct accggaaatg cagtaatatc
    17161 actagtaatt gggatattag cttcagtttt catcaactgt gccactgtat cagtataaga
    17221 ttcaggaaat tgtttctcaa caaacattgt tttgattcgt tcattgttca catcgtgacg
    17281 ttcattataa cctagtaata ctccgtcact tcctatgata taggtgttgc tgtcaagaat
    17341 accacctagt gtgacagcca ttttatcgta aggcaaatct gcttgcatgt caaaagtatt
    17401 cttttgctgc aaaagtgtgt ttatcttgcg ggttttcgtt agcaagtctg tcattgtcgt
    17461 cctccgattt tttataaaat atagcgactt aaatcttcat ttgccgcaat tgagccaagt
    17521 ttgtcatcca catatgcttc tgtgatcgtt atttctccca tttgcatatc tggtgcttca
    17581 aataatagat cttctagtag tttttccaaa atggtatgca gtctgcgtgc gccgatatta
    17641 tctgtgtcac ggttgacatc gaaagcgatt tgagcgatcc gttcaattgc ttcttttgta
    17701 aacgtaactg acacattttc tgtaccgatc aacgcgacgt attgttttac taaagcatta
    17761 tttggttcgg tcagaatctt cacaaaatct tcagctgtca aatcatctaa ttctacccgt
    17821 atcgggaagc gtccttgaag ttctggaatt agatcacttg gtttagacaa atggaaagct
    17881 cctgaagcaa tgaataggac gtgatctgtt tggatgatcc cgtattttgt gtttacttgt
    17941 gaaccttcga caattggcaa tatgtctctt tggacgcctt ctctggatac ttctccagat
    18001 tgctgtgatt tagaagtgat cttatcgatt tcatcgataa aaataattcc actttcttcc
    18061 gctaatttga ttgcctcact atggatatca gcatccttga ctaattttgc tgattcttcc
    18121 ttgatcaata actctaaagc ttctttgact gttaccgtcc gttccacttt tttcttcggt
    18181 gtcaaagcac ctagagtttc atttaggtcg atccccattt gctccaatcc attgttcatc
    18241 attggtgcag gtttcttttg gtcttcaact tcaatggtta cttcacgact atccagtaaa
    18301 cccttttgta gttgttccaa gactgtacgg cgattgattc gaatatcttc tgtcacttct
    18361 tcttgcggtt cagcggtatt ttgctgacta gcattaaaaa gattcatcat ttgttcaaat
    18421 tgattagtgg attgtttctg ttcttttttg ataccaggta ccaagatttt taccaaacgt
    18481 ttttctgcat tttttttagc acttgtgtat acacggctgt actgctcttt ttctacgatt
    18541 tggatcgcat tttctactaa atcccgaacc attgattcta catctcttcc gacataaccg
    18601 acttcagtaa attttgttgc ttcgactttg acaaatggtg cattgacgat ttttgccaaa
    18661 cgtcgtgcga tttctgtttt accgacccct gtaggtccga tcatcagcat attttttggt
    18721 gtaatatctt gttgcatttt ttcatctaac tgcaaacggc gatagcggtt acgcaaagca
    18781 acggcaacag atttttttgc tgcgtgctgt cctacgatat attcatctaa ttcacttacg
    18841 atttgtcttg gtgtttgttt taattcatac atatctactc acctcttaca gttcttctac
    18901 gataatatta tgattagtaa agatacagat atctgcggca atatttaacg cattttctgc
    18961 aatttctttc gcactcatgt cgttttgagc atagtttttc attgctcggg ctgcagccaa
    19021 cgcaaaattc cctcctgatc cgatagctaa gataccatca tctggtgtga taacttctcc
    19081 tgtacctgaa acaagaagca tttctttatc attcattacg atcagcatcg cttcaagttt
    19141 ttgcatagat tgctgtgtcc gccattcttg agctaattcg acagctgcgc gttgtagatt
    19201 cccgttatat tcgtttagtt tgccttcaaa tttttcttct aaagtaaacg catctgcaac
    19261 acttccggcg aaacctacaa caacttctcc attgtagata cgtcgtactt tctttgcagt
    19321 gcctttcatg acaacagatt cacccattgt tacttggcca tctccagcca tagcaaattt
    19381 cccgtctttt tcgacggcac agatagtggt tgaatgaaat ggtgattcga ccatgtttgt
    19441 ttcctcctaa atttgttctt ttccttattt ttacgctctt ggatggaaag aacgatagtt
    19501 tttttgtaaa ctttcttttg ttacatgggc atagatctgg gtagtcgata aattggcatg
    19561 tcctagcaat tcttggactg tccgcaaatc tgccccatta tttaataaat gagtagcaaa
    19621 tgtatggcgc agcatgtgag gatggatatc actatccaaa ctgctttttt tgatcatttg
    19681 attcaagaca tattcaattc cagtaggcgt gattggtttt ccatgatgat tcacaaaaac
    19741 gatatcatgc tcttcgtgat attttgtcat caatacctgg cggccttccc tgaaatattc
    19801 ttgcagagca tcttgagcaa aagaacctag aggagcatat ctctctttgt tccctttacc
    19861 atggatcaac atgacctctg aatcaaaatc aatgtctgat accgacaaac tgctacactc
    19921 acttaaacgg atgcctgacc cgtaaaggac ttccagtaaa gcgcggttcc tcaaatccaa
    19981 aggactatct cctgctacgc tgtcaaaaag tgcttgcatt tcattttcat aaaaaaaacg
    20041 tggcagtctc aaatttttct ttttgagatg aacgtaagaa aatggatttt cttttatcac
    20101 ttcttctttg agaagatact gataaaacga acgtaggctg gctatcttgc gactgataga
    20161 atttctgcta tagctaggac gatgatcagc taatgcactc aaatagacac gaaccgtaaa
    20221 atattccgcc ttcagcagat cagcgtctcc agattcctcc aaaaaatgtt tgaaatggag
    20281 aatgtcttct tcatatgctt ctttcgtttt ttctgaatat cctctttcga caatcaaata
    20341 acgcaaaaat ttttgttccc acgtggcttc tgtcattttc tcaccttcct tttgtcccag
    20401 agaataattt agcacagcgt ttataaaaag acaaataaat tgtcagaatt taaccaatat
    20461 ttctaagttt gttcacaatt ggcgcaattc ctcacatttt gtttgagaaa ttcttaagaa
    20521 aaacgcaaga aaaagagcga gactatccgt ctcaccctct tcatggagtt tccttactca
    20581 tttgggattg cagttttttc taattctgtt tttattgcct cattagcttt taaagctcgt
    20641 tgtgcaattg cttcatatct tgcttttttg tctcgaatgc gctctggcag ttcaggaaac
    20701 agaccatagt tggcattcat cggttggaaa tgttttcctt ccgcatgagt aatgtagtaa
    20761 gccatacttc caatagctgt ttcttgtgga aactcgactg gttccatttc ttttgctaag
    20821 cgtgcggcgt tgattccagc aagtagtccg ctcgctgcac tttcaacata gccttctaca
    20881 ccagtcatct gacctgcaaa gaacagatct tcgcgtttcg ttgattggta agtcggtttc
    20941 agcaattctg gcgaattcat aaagctgttt cggtgcatca caccatagcg aacgaattca
    21001 gcattttcta acccaggaat catccggaaa acacgttttt gctcgcccca tttcaagtga
    21061 gtctgaaagc ccacgatgtt gtataaagaa gctgcagcat tgtcttgacg cagctggata
    21121 acagcataag gacgtttgcc agttttagga tcttccaatc caactggttt cataggtccg
    21181 aaaagcatcg ttttgaatcc tcgctgtgcc attacttcaa ttggcataca tccttcaaaa
    21241 tatttttctt tctcaaaaga tttaagttct gccacttcgg cattgattag tgcctcatgg
    21301 aaagctttga attcctcttc tgtcatcgga cagttcaagt aagctgcttc tcctttgtca
    21361 tagcgggact tcaaatatac tttatccata tcgatcgtag ctttgtctac gattggtgca
    21421 gctgcatcat agaaataaaa accatctgaa ccattgaatg ctttgatgct ttctgctaat
    21481 ggatgagaag tcaaaggtcc tgttgctact acggtgattc cttccggtat ttcagtaatt
    21541 tcttcgttta tcactgttac taatggatgc tctttgactt ttcgggtaat tgtttcagaa
    21601 aaagaatcgc ggtcaacagc taaggcacca ccagctggca ctgctgtttc atctgccgaa
    21661 gtgattacta ccgaattcaa tcggcgcatt tcttctttca ataaccctac tgcattagcc
    21721 aaactgtttc ctcttaatga attggaacat actagttcag caaaattact tgtgtgatgc
    21781 gcctcagtcg atttgactgg acgcatttca tataaacgga cagggactcc tgcttgtgcg
    21841 atctgccaag cagcttcact tccggcaagc cctgctccaa caacatttac cattttcaat
    21901 gaaagaacct cttttctatt atttttggat gtcttcttcg taatctccat ttacacagac
    21961 tacttgttta ccgcctttga ctttcttctc gaccagatat ccgtcacatt ttggacaatt
    22021 tctgcccact ggtttatccc aagaagtgaa ttcacattct gggaaacggg aacaaccata
    22081 gaatagtctg tttttcttag attttcgttc aatgacttgt ccttcatgac atactgggca
    22141 agtcacaccg atttctttta cgattggttt cgtattccga caatcaggaa aattactgca
    22201 agcataaaac ttcccatatt tccctaattt tatcaccatc ggatgtccac agacatcaca
    22261 atcgaatcct gctggttcat ctttgatttg aattttttcg attttttctt ccgcattcgt
    22321 cagttctttt tcaaacggtt tatagaaacg atcaaccact ttgacccacg cttccgtgcc
    22381 ttcttcaatt ttatccaaat ctccttccat ttctgctgtg aaatggatat cgacgatttg
    22441 agggaagaat tcacagatta atgaattgac aatttctcct agttcagtcg gctcaaatct
    22501 tttttgtgtc aattttacat agtagcgtct ttggatcgta tcaagtgttg gtgcataagt
    22561 ggaaggtcgc cctaccccgt tttcttctag tgcacgaatc aacgtagcct cactaaaacg
    22621 tgctggtggt tgagtaaaat gttgttttgg ttcaatatca acagattgga ctacatcgcc
    22681 ttcttcaagt tctggaagaa tattttcttt ctcttctttg ccgtcatctc taccttccac
    22741 atacacttgc atgaatcctt tgaatttgat ttttgaaccg ttcgcaatga agatgacacc
    22801 attttgttct aatgtcactt tcattgtatc taaaatagct ggtgtcatct gactagccac
    22861 aaaacgtgac cagatcaatg tataaagttt caattgatct ttatctaaat attgtttgag
    22921 ttcatctggt gtacgaagca cactcgttgg acgcacagct tcatgggcat cttgtgcgcc
    22981 ttgagcattt ttgatctttc ggtgaccatg tctagaaaat tcttttccat acgtatcttc
    23041 aataaatgct gcggcctctg cttttgctgt atcggcaatc cgggtagaat cagtacgcat
    23101 gtatgtaatc aagccgactg ttccttgttt acccaaagtg attccttcat ataattgctg
    23161 tgctaccatc atggtttttc ttgttcgaaa gtttaactta cgggcagctt cttgttgcag
    23221 gctacttgta gtgaaaggat tcgcagggtt tcttttgcgc tctttctttt ctactttttt
    23281 aacttcgaag tctgttccat caattctagc agtgacttct tttacacttt gtgcatctgg
    23341 taatttcttt ttcttaccgt cgattcccca gaaatttgct ttgaatttct tgcgagcttt
    23401 tttaaaatta ccgtcgatgc tccaatattc ttctggaaca aattctcgga tctctttttc
    23461 acggtcaata ataattttta aagcgacaga ttggacacgt cctgcactta accctttctt
    23521 gaccttacgc cagagaatag gactgatcga ataaccaacg attctgtcta aggtacgacg
    23581 agcttgctgt gcatctacta aatctacatc gatcgttctt ggttccttaa aagctgcttt
    23641 gactgcgtct tttgtgattt cattgaagac cacccgattt ttttctttca gatccaaccc
    23701 aagtaggtac gctaaatgcc aagcaatcgc ttctccttct ctatccgggt cacttgccaa
    23761 gtaaactttt tgagcttttt ttgctgcggc tttcaggctt ttgatgacat cgccttttcc
    23821 gcgtatagaa atataatggg gttcataatt gttttcaaaa tcgataccca ttttactttt
    23881 cggtaaatct cttatatggc caacactggc cattacttta tagtttcgtc caagatattt
    23941 ctcaattgtc ttggcttttg caggtgattc tacaatcact aaatatttat acgccattac
    24001 tgaatggact cctttccatt gtcgtgttcc tactattaaa agaggattca tacaaatcgt
    24061 agctaatcat atccattcag aaaacgtttg tcaagaaaag caaatgaaaa gtatggtgtt
    24121 attctttaaa ttctgccaag atatcttcac ccgaaaccgt gcaaactgca ccgtcttgga
    24181 tcaaatgatg acatccggta gaatgttctg ttaaaatatt tcctggaaca gaaaaaactg
    24241 ttcttccaag atccaatgct tgctgtgctg ttatcagaga gccgcttttt ttcttggctt
    24301 caaagacaca tgttccttta gacaaagctg cgataatccg atttctcatt ggaaaacaat
    24361 gctttttcgg gccggattga ggaggatatt cactcaaaag aagatgatca tttgcaatca
    24421 cctcttgcaa gtcacgattt tcagcaggat agacttgctc tatccctgtt ccaatgactg
    24481 cgattgtccg tccaccatga gagattgctg cttggtgacc gtatgtatct atccctttcg
    24541 cgcagccact gacgatcaca tatccagctt ctatcactgg ttttagtaaa taataaactg
    24601 tttttttggc taaagcagaa gtgtgtcttc caccaacaac gcttaacatg ttacaagaaa
    24661 gtaaagatag atcaccatta taaaacagta caattggcgg ataggtcaag tgaagcagct
    24721 gagggggata tttgggatca aagcaagtga taaacgactg tttcgattgg attttgcccc
    24781 agttagcgtg aatttcctgc caacttttgc gaaacagctc ccggtgtttg gttattttac
    24841 ctatcttaat gatgtcttca aatccgattt ctttataagg atactgcatc atccagttag
    24901 caagtgccca ttttccttga ctactgattc cttttgtata agcaagtttc aataaatttt
    24961 cttctatttg atacatcata aaaccgcctt tcgtctctct ttctaataag atacgtaaaa
    25021 gcggctgttt tttattgtaa atattttttt acaggtgcaa aacttttacg atggattggc
    25081 gtaattcctt gtcgttcaag tccttctaag tgttcctttg ttccataacc tgcattatgc
    25141 gagaaaccat atccaggata aagcacgtca tattctttca tcaattggtc tcgatatact
    25201 ttagcaatga tacttgcagc agcaatcgac acagatcgtg catccccttt gattattttt
    25261 tcctgctgaa taggcaaatc caaggtcatc gcatcgatca acaaatagct tggtacaatg
    25321 gacaattttt ctactgcttg gatcatcgct aatttacttg cttcataaat attcacatca
    25381 tcgattgtct gctgatcaac gatcccgatc ccgattgcca gggctttttc ttgaacttgt
    25441 tgataaagaa gttctcgctt tttctctgat aattgtttgg agtcattcag ccctaatatg
    25501 atctcatttt tcggcaagat aactgcagct gccacgacag gtccagctaa tggtcctctt
    25561 cctacttcat caatgccggc aatccattgg tgaccttgtg cgtacccttg cttttcaaag
    25621 caaaacattt cttcatattt ttccttaatt aatgcttcct ttttcaatcg tcgttcaaaa
    25681 gaagccagtg cttgctgcac gcctttgcgt ggatcatttc ttaatgattc tagtgtagga
    25741 tcatctattc tctgtatgct tttcaatcgt tctttgatac tttggatgga ttcagtcatc
    25801 acttttttgc tccatttctt cgcaacgatc cagtgtgtat cgtccaagtt ttccttgacg
    25861 aatatcatgc acgactaatt cgctgccacg atcatagtct tctttatacc cacgttttgc
    25921 tgtaatatcc atcagtaatt ccggtgccaa tttttcttct tgctcgattg ataaaccata
    25981 acgatcgctg attcttcctg aataatatcg ggcaaaaaag tctaatccgt aaatcgctaa
    26041 atcatctaaa tggacaagct ggtctttgat cgcgcctgtc agcgctagtt ttttgccgat
    26101 ttcttcatct tcaaatttgg gccataaaat ccctggtgtg tctaacaatt ccaactcatt
    26161 acctaaacga agccactgct gaccttttgt tacgccaggc ttatttcctg tctgtgcaat
    26221 tttcttgcct actagtcgat tcatcaacgt agatttccca acatttggaa tacctaagac
    26281 cattgcccga atagcgcgag gcttgatccc ccgagcagca tctcttgccc gtttttcttt
    26341 caatgcttct ttagccgcag gtactagacc tttaattcct ttactttctt gtgccgtgat
    26401 agctaatgca agatggcctt gtttacgaaa atattcttgc cacagctttg tctgttgtgg
    26461 atctgccaga tccgctttat tcaataaaac aaggcgaggt ttttgctgaa tgatctgatc
    26521 tagcattgga ttacgagaag ataaaggtaa tcgtgcgtct accaattcaa atacgatgtc
    26581 cacatatttc attttttcag aaacttctcg tcgggctttt gccatatgtc ccggaaacca
    26641 ttggatggtc attgttctct ccattccttt cataccggaa tattccccgg ctttacttgt
    26701 ttttttcttt ttcaatataa attcac
//
LOCUS       DAXLPI010000026        26422 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXLPI010000026 DAXLPI010000000
VERSION     DAXLPI010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.026"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            18..719
                     /locus_tag="ACF3T5_000975"
     CDS             18..719
                     /locus_tag="ACF3T5_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFD3631602.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1328..2344)
                     /gene="tsaD"
                     /locus_tag="ACF3T5_000976"
     CDS             complement(1328..2344)
                     /gene="tsaD"
                     /locus_tag="ACF3T5_000976"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFD3631603.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2341..2805)
                     /gene="rimI"
                     /locus_tag="ACF3T5_000977"
     CDS             complement(2341..2805)
                     /gene="rimI"
                     /locus_tag="ACF3T5_000977"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFD3631604.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2812..3354)
                     /gene="rimI"
                     /locus_tag="ACF3T5_000978"
     CDS             complement(2812..3354)
                     /gene="rimI"
                     /locus_tag="ACF3T5_000978"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFD3631605.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3338..4060)
                     /gene="tsaB"
                     /locus_tag="ACF3T5_000979"
     CDS             complement(3338..4060)
                     /gene="tsaB"
                     /locus_tag="ACF3T5_000979"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFD3631606.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4251..5231)
                     /locus_tag="ACF3T5_000980"
     CDS             complement(4251..5231)
                     /locus_tag="ACF3T5_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631607.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5255..6739)
                     /gene="galT"
                     /locus_tag="ACF3T5_000981"
     CDS             complement(5255..6739)
                     /gene="galT"
                     /locus_tag="ACF3T5_000981"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFD3631608.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6751..7740)
                     /gene="galE"
                     /locus_tag="ACF3T5_000982"
     CDS             complement(6751..7740)
                     /gene="galE"
                     /locus_tag="ACF3T5_000982"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFD3631609.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            7988..8155
                     /locus_tag="ACF3T5_000983"
     CDS             7988..8155
                     /locus_tag="ACF3T5_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFD3631610.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8217..10028)
                     /locus_tag="ACF3T5_000984"
     CDS             complement(8217..10028)
                     /locus_tag="ACF3T5_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFD3631611.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10025..10390)
                     /locus_tag="ACF3T5_000985"
     CDS             complement(10025..10390)
                     /locus_tag="ACF3T5_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFD3631612.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10553..10948)
                     /locus_tag="ACF3T5_000986"
     CDS             complement(10553..10948)
                     /locus_tag="ACF3T5_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFD3631613.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(10966..11928)
                     /locus_tag="ACF3T5_000987"
     CDS             complement(10966..11928)
                     /locus_tag="ACF3T5_000987"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFD3631614.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11928..12140)
                     /locus_tag="ACF3T5_000988"
     CDS             complement(11928..12140)
                     /locus_tag="ACF3T5_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFD3631615.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12161..12859)
                     /locus_tag="ACF3T5_000989"
     CDS             complement(12161..12859)
                     /locus_tag="ACF3T5_000989"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFD3631616.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12879..13421)
                     /locus_tag="ACF3T5_000990"
     CDS             complement(12879..13421)
                     /locus_tag="ACF3T5_000990"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFD3631617.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13553..14557)
                     /locus_tag="ACF3T5_000991"
     CDS             complement(13553..14557)
                     /locus_tag="ACF3T5_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFD3631618.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14554..15543)
                     /locus_tag="ACF3T5_000992"
     CDS             complement(14554..15543)
                     /locus_tag="ACF3T5_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFD3631619.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15540..16346)
                     /locus_tag="ACF3T5_000993"
     CDS             complement(15540..16346)
                     /locus_tag="ACF3T5_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631620.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16512..17468
                     /locus_tag="ACF3T5_000994"
     CDS             16512..17468
                     /locus_tag="ACF3T5_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3631621.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17545..18063)
                     /locus_tag="ACF3T5_000995"
     CDS             complement(17545..18063)
                     /locus_tag="ACF3T5_000995"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFD3631622.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18151..18300)
                     /gene="rpmG"
                     /locus_tag="ACF3T5_000996"
     CDS             complement(18151..18300)
                     /gene="rpmG"
                     /locus_tag="ACF3T5_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFD3631623.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18528..18974
                     /locus_tag="ACF3T5_000997"
     CDS             18528..18974
                     /locus_tag="ACF3T5_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFD3631624.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19169..21064)
                     /gene="asnB"
                     /locus_tag="ACF3T5_000998"
     CDS             complement(19169..21064)
                     /gene="asnB"
                     /locus_tag="ACF3T5_000998"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFD3631625.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(21389..22363)
                     /gene="bsh"
                     /locus_tag="ACF3T5_000999"
     CDS             complement(21389..22363)
                     /gene="bsh"
                     /locus_tag="ACF3T5_000999"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFD3631626.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(22697..22784)
                     /locus_tag="ACF3T5_001000"
     tRNA            complement(22697..22784)
                     /locus_tag="ACF3T5_001000"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22750..22752),aa:Ser,seq:cga)
     gene            complement(22929..23495)
                     /locus_tag="ACF3T5_001001"
     CDS             complement(22929..23495)
                     /locus_tag="ACF3T5_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3631627.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            23751..25081
                     /locus_tag="ACF3T5_001002"
                     /pseudo
     CDS             23751..25081
                     /locus_tag="ACF3T5_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
     gene            25047..25397
                     /locus_tag="ACF3T5_001003"
     CDS             25047..25397
                     /locus_tag="ACF3T5_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631628.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(25489..25908)
                     /locus_tag="ACF3T5_001004"
                     /pseudo
     CDS             complement(25489..25908)
                     /locus_tag="ACF3T5_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(25909..26253)
                     /locus_tag="ACF3T5_001005"
     CDS             complement(25909..26253)
                     /locus_tag="ACF3T5_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFD3631629.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
BASE COUNT         7828 a         5520 c         4533 g         8541 t
ORIGIN      
        1 aaggagagga ctttttaatg cgtgttaata aaatcgattt tcacttttca aaactcagat
       61 atcaacctga ttttgaacat ttctcggatg aagagttcca acagatcaag gaacatactg
      121 ttcttcgttc ttataaaaaa ggacagatcc ttttcgacga tggtgatccc agacaacggt
      181 tgtatatctt gaccaaagga ctcgttcgtt tagaacgtta tgatgaaagt gcaacttatt
      241 actactatga ctatgtgaat acaaataacc tttttcctat gtcaggaatg tttggagaag
      301 agacctacac ttatacagct caagcgatga ctgatattga aacattctac cttcctgttt
      361 cctactatga aaaattagca cgaaaaaatc ccgaacagtt gatctatatg atacaaaaaa
      421 tttctaaagt agttgaaata catgaattgc gtatccaaat cggattgacc tctagcgcct
      481 ttgaccgggt aaaacaaaat ttattcattt tgaaagaaga attgggcgta ccaacaaaag
      541 atggtcatat caccattcct tatccgatca ccttaaaaga attagcagtg aatagcggca
      601 cgacacgtga aactgctgga caagtaatca aacaactacg tgattccggg ctgttagatt
      661 atgacaaaaa gcgttttact ttctgtaaga cagctactac tccagctatg agagaataac
      721 gcgaacaaaa ataagcagtg accctgataa atatcagggt cactgcttat tttcctccct
      781 aaaacttctg aaagatccgg cagttttaga tcactggaca agactcaaag attttctatg
      841 tgtgtttcag ttatgtaata ttcactgttt gcaaaaaata ctgttagatt aactagatga
      901 agcgtcttgt cctcatccga tctagattat tcgatattcc tcgacaataa acgattactc
      961 agtgagggca atcagaggaa cggataatga taaataagct gtaaaaaata gcaaaataac
     1021 aataatgatt aaaagtatgt aaaaaatagg gctttgacaa aaacgtgtca cagccctatt
     1081 tcgattaaac ggtgtgtacc tgtcaactct tcggtcacaa gtcataatgt tcataaacat
     1141 gaggagctgt tttttgaaat tgttccttgt gaatcagagt taaacgacag gcttacgaac
     1201 tcttgattaa acggcgttca aaagcttgca tacgcggtaa taagaacagc caggtcaaaa
     1261 acatgagaag ccgtttttcc tgggctgttc ttattacttg atgcagggca gctttttcac
     1321 aacctcttta ttcagataaa accaagcttg gatcagcatt caactgatag ctagcaaaat
     1381 ttcttttttg catttccaca tgtgctgctg ctccaatcat tgcagcatta tctccacaaa
     1441 gtcgaagagg gggaatcact agttctacct cagggagttt agctgccaaa gcatcctgca
     1501 atccttcacg caacccttga ttggccgcta cacctccagc tataatcagt tgcttgactg
     1561 gataattttg acaagctcga agtgttttag taatcaatac atcgatcaca cttgcttgaa
     1621 agctagctgc taaatcattt ttgtctagat cttctccacg ctgttgtgca ttatgcacca
     1681 aattgataaa tgcacttttt aatccgctaa aactgaaatc ataattatct tcatggatca
     1741 tcgctcgcgg aaaatggtaa ttatcttttc cctgatgagc tagctgatcg atttcttttc
     1801 cgcttgggta agaaagacct aatactcttc caactttgtc ataagcttct cctgctgcgt
     1861 catctcttgt ctccccaatg atttcatatg atccatcttc ttgcatatag actaattctg
     1921 tatgtcctcc gctaacaaga agtgccatca atggaaactg gaatggttta accagacggg
     1981 ctgcatagat atgccctgcc atatggttta caggaataag cggcaattgg tgtgcccaag
     2041 caaaagcctt agctgcagaa ataccaatca gcaacgagcc gacaagtcct ggtccataag
     2101 tgacagcgac tgccgataaa tcttctgccg acacaccagc ttctaccagt gcatcttcca
     2161 agcataaggt gatttgttct acgtgatggc ggctagctac ttcaggtact acgccaccaa
     2221 aacgtttatg actgtttatt tgagaagcaa cgatgttaga taagatctct gttccatttc
     2281 taacgaccgc tacactcgtt tcatcacaac ttgattcaat cgccagaatc aattcttcat
     2341 tcatttcatc gtctccgttt ttaactttct gctcataatg atcgcatctt ccgtcggatt
     2401 gcgataatat gatttccgtt tacctaatat acgaaacttt tctgattcat aaagttttct
     2461 tgctgcttca ttagatagac gtacttctaa aaaaacatgc agcgaatctt ttatttgttc
     2521 cgtaatcagt aattgtaaca aatgccgtgc atgccctttt ccttgttctg aaactgcaac
     2581 agcaatattt gtgatctcga cttcatctat tactttcgag tatccaataa agccacagat
     2641 ctggttatct tcagctaaaa gcagataatc agagtgaggc tgttggatgt ctgccaaaaa
     2701 ctgcattttc gtccaaggtg aaccatgtgg ataagcattt tcgctaatca tccatacgca
     2761 ttctgccaag tacgcttcat caaaatcttt ttttctgcaa atcaactaat ctcactcatt
     2821 ttctaaaagt tttatcatat ccaacgcatc ttcttttgtt tcagtgtaat atcttttttt
     2881 gatcgttgtt gcttgaaacc ccaatttccg ataaagtctt tgtgcatctt gattactcat
     2941 acgcacttct aatgacatcg tctcacagcg attcattata gcaaagttct cgatttcgtc
     3001 aataagcagc gaaccgattc gtttacgctg atatgccggt cgaacggcaa tattcgtgat
     3061 atggcaatct aaaccgaacg ctcttgaacc gataaaacca atcattcttc cattatcaaa
     3121 gatacctaga tatagatgag gaagcggtga cttcaattct gatataaagg cactttttgt
     3181 ccatggcaat tcgccattat agacttctcg ttcgacatcc agcagctctt tgatatcttc
     3241 tgtagtcaat tctcttagta cgtaacgacg atcgatttgt tcgactgttt tttctgtata
     3301 aatcatttta tcttgaaaaa agcttctggc atacgctcta aattttttca acataacttt
     3361 cgtctccagg tgtgtgcgtc tccaaccatt tttcttctgc ttctactcgt tttaaatagc
     3421 gaggaagaaa agcttgtttc tctgcaactg gttcaactaa agcacctaat tgagccacag
     3481 ttacaccact tggaatatcc catgctggaa ctgtattaat catcgcacta ggcaatattt
     3541 cctttatttc agagaagaat ttgtgacaat cactcccgac gaaataaata gcttcattca
     3601 gcttttgtag ttgtgtgaac aaatcagaag cagaaatatg tacatcttca agtacagttt
     3661 ctaatctatt ttctttccag cggtatgccc cagcgtatac attttttctt cgagcatcaa
     3721 acatgggcac aataatatta tccacaccga tgcaattagc cgcgatggtt ttcagactag
     3781 atactcctac tagctcttta tctaatgtat aagcaatcgt ttttgctgtc gttacaccta
     3841 tacgaagccc tgtataagaa ccgggaccat cagaaaccgc gattcggtca atgtcttttg
     3901 gacttatttg aagatctgca aacaattggt cgattgcagg catcagtgtt acactgtggt
     3961 tgcgtttgat atttgtttgt atttgtccaa gaatttgttc gtcttctacc acaccgatcg
     4021 ccaaagtttg gtttgcagta tctattccta atgtaatcat atatttgatc ctttctgcta
     4081 tgacattctc gccttccatt gtagcatact tttttattct gaggatgaaa ataccaaagg
     4141 caaaactagt acaataatac atgattatct gctggcgggc aaattttgcc ccttcctgaa
     4201 acacaaaaaa gctgcggtat cactccattt tcccacagct tttccatctt ttatttcgtt
     4261 gattctcgaa taatcaactc tgtttccaca ataatctttc ttgaaaccgg tgctttggaa
     4321 ttacttaatt gaagaatcgt ctccactgct agctctccca tccattcggt ataaactttt
     4381 acagtagaaa gcgagggcgt tacatatttc gctacactga catcattgaa gcccatcaca
     4441 gaaatatctt ctggtaccac taaacctttt tcttgaatcg ctttcagtgc gccaatcgct
     4501 aatgcatcat tagctgcaaa aagcgcatca ggaacatgcg gattcgtttt taaatattgc
     4561 ttcactgctt gatatcctga ctcgacagaa aaatctgctt cgataattgg accttcgtcg
     4621 tagccttttt ctgccaaaac ttgttgaaat gccataattc tgggatcttt tagccgctga
     4681 tggtttttct tcgtatattc tgttccagca agcatagcga tatgttgcgc tttatgggag
     4741 acaaagtgtt caatgacttt ttgaacactt gacttaaagt cgacaactag agagtcgatt
     4801 ccttgagaag tgccgtcaga atctaccaaa agcaaggcat tttgggatcc tttcatttct
     4861 aataactgag cttcgtcgaa ttttcctagt gcgatcgttc catctgccgg aagatcacta
     4921 atggaatccc acgtttcttt caataaagta acgtttgctt cttctgcctt tttttcgatt
     4981 cctaggcgaa tagataaata ataaagatct tcaagctctt ccgtatcgtc gtaccactgg
     5041 atcaggcgaa gcgtttgctt agtatttgct gcatttttac gatgtttcgt ataatttaag
     5101 cgttctgctg cttcaaaaat tttttgcttt gttgctaagc caacggaaag atcaggatca
     5161 tagttgagaa ctcttgaaac agtagcagga gaaactcctg ccaactgcgc aatgtcttta
     5221 attgttgcca tttgtctctc ctcctttttt acgtttataa aatcgcagta aatcgttcaa
     5281 acgctgcctg tcctttttca tctcgcttga atactccagc gtcttccagt acacgtgcaa
     5341 aaacctgacc tacagatttt tgtacgacat cggtgaccgt atctttcgtc aaatcaggat
     5401 gttctttttt taattgttct gcccacggac gatgatattc agccatctgg ttttcttctc
     5461 ctaataagaa atgctctact tctactagtt ctggtttcaa acgaggtggt aaaacagcaa
     5521 gccccattac ttcgatcaac ccaatgttct cctttttgat atgttggaca tctttatgtg
     5581 gatgaaaaat cccatcagga tgttcttctg aaacattgtt gtctcgaagg acaaggtcca
     5641 actcaaagta attgtccctt cttcgtgcaa ttggcgtaat cgtatggtgt ggtgtaccat
     5701 ctgctgaaaa agctctgata gatactgttt catccgaata tgctttccat ttttccaata
     5761 ctgagttagc tgcttcaata agcaagtttt tctctgttga ttgcaaacgg atcacagaca
     5821 tcggccattt cacgactcct gcgatgactt gcggataatc ttctagtttg ataagttttt
     5881 ctatcggtgc ttttgccata ggaaattcat gacgtcctgc ctgataatga tcatgagaaa
     5941 gaatcgatcc tccaacaatt ggtaagtcag cattcgaacc aacaaagtaa tgaggcaaga
     6001 cttcagtaat ccgaagcagt ctagcaaatg tttcctttgt gattttcatc gggcggtgtt
     6061 gttctgataa aatgatcgaa tgttcattgt aataagcata aggggaatat tggaatcccc
     6121 agctttcacc gtctagattc attcgaataa tccggtgatt ggtgcgggct ggatagttca
     6181 agcgacctct atatccttcg ttttccatgc aaagcatgca ggtaggatag tctactttta
     6241 ccgcctcgcg ttctgctgcg atttgttttg gatctttttc tggcttagat aaattgattg
     6301 tgatttccag ttcaccatac tccgactctg ccggaaattt gatgtttttt gcgatggctc
     6361 ttgttttgat gtagtcattt ttcttactta aattaaagaa atactcagtt gcttcctctg
     6421 ggcttttcga atagtgctgt gcaaagaatg cattgacgac tgaaggtggc ggtgtgagaa
     6481 aatccattaa ttgcgcttca aaaatctctt gttctgctgg caattctgaa agaacaccat
     6541 tttctcttgc cttagcgaca agctgatcca ataagtctac agattcttcc ttaactggtc
     6601 ttacttcaat gctttctaga gcatcttctc ctaccatccc taaaagacga ttctgtaaat
     6661 ataagcggtc catttccatc catccgccag cttcgatcgc aagtgtggca aaatcacaaa
     6721 tcgtttgact gattgacata tctcattccc ctattcttca taaccatttg gatggctgac
     6781 atgccaatcc catgctgttt tgataatcgc tttgatatct gtgtattcag gtttccagcc
     6841 cagaatctct ctcgcttttt cacttgaagc gactaatcgg ctaggatctc cagctcttct
     6901 tggtgctatt tctgctggaa tttcttttcc tgtcacttct ctagctgctt ctagcatttc
     6961 cttgactgag tatcctttgc tgctgcctaa attgaagaag ttgctttcat ttccttcttt
     7021 caagtattcc aaagctaaaa tatgtgcagc aatcaaatct tctacttgaa cataatctct
     7081 gatacatgtt ccatctggtg tatcataatc gtctccataa acagccaagg cttttctttg
     7141 accaagagct acttgtaaaa tgattgggac aagatgcgtt tctggtgtat gatcttctcc
     7201 gatagatgca tctgcttttg ctccggctac attgaagtag cgcaaagcga catagcgcat
     7261 gccatatgct tggtcacacc acttcatcat tttttccatc atcaatttac tttcaccata
     7321 cggattttta ggattagctg gtgtatcttc tgtgattgga gactctttcg gctctccata
     7381 agttgctgca gtagaagaga atacgatttt attcacgttg aactcgtgca tgacttcaag
     7441 taagatttgc ataccgtata cgttgttgtt gaaatacatc aatggttttt cgacagattc
     7501 tcctactaaa gagcttgcag caaaatggat tactccttca atcggttcct tttcaaatac
     7561 ggaacgtaga aattctttat cccggatgtc tccttcataa aaatgagcat ctggatgtac
     7621 tgcttgtttg tgacctgtga gcaaattatc taccacagct acttgatacc ccttttgaac
     7681 tagctggtct acagcgtgcg aaccgatata accggctccg cctagtacta aaattgtcat
     7741 aaaaaacact cctttgatta gtaaaattgt gtttgttgat tctatataat ttaaaaaacg
     7801 aagtttactt ttatggtgaa aacaaacaaa gtaaaatttc gtaaattcat atggtaaatt
     7861 tataataaaa catttagtaa acattttcaa gggttttttg caaaggttta caaacaaacg
     7921 taaaactagc ctttttttct cttttgttcc ataatagata taacaaataa taaatgaggt
     7981 ggattcaatg aaaaaatttg tatctggaat attagtagga agtctggcaa cagccgctgc
     8041 cgttgctggt ttggttgctt ctgtgaaaaa aacagtgatc gatccaattg acgaaaaaga
     8101 agcaatgatc gaagaaaacc gtaaaaaagc aatgcgtaaa cgcgtttctc gttaataatt
     8161 atttaaaaat acgcatgaag actttctaaa aaacagataa gtcatcatgc gtatttttat
     8221 ttgtcagtca atataagttg tgctatttta tctgcctgtc tcagattcca tggatctgta
     8281 taaggtctag ctactgctgc agttagccaa tccacacctt caacaggtat tccatagcaa
     8341 taaaagtcag actggatttc ccctgaagca tcaatcacgc gattcgtacc tcgctcaaca
     8401 tctatagctt ctgaatgata acttcctgta ttatctgaaa atgaaaaagg gcgaacaagt
     8461 ttgttttcct tcaaattttt tatgacagga ttacttgaac gagaaaagct tgtttgtggc
     8521 aatcgagctt caatcaaata gcgactgctg atttctttat cttgtagaga aataacatag
     8581 cggttgtgct tttgctgaat atcaaatggc ggttcgatca aatgaaaaat acccgcatct
     8641 atcagtgctg ctaattcttt cgtccgaatg attggcggac cgatcgttaa aaagctattt
     8701 agatgagtaa accatcccca tagtatttct ttgtattcct tagcagaaaa aacgtgccat
     8761 tcaatagcct gccggattgg atcacgccag tcttttaatg catcaaatgt tgaagcaaat
     8821 gctcctgtgc aattgccttt ttccgcttct tttatctgcc cttcaataaa tattcggcta
     8881 gcttctgaaa agggctcatt ctgataggca agaggagttt ctaaatacga ccaatcccat
     8941 ctataaggga ttagtgctgg atgctttccg agacactcct ctgaagagag ggagagaaaa
     9001 tcatgctcaa aatttttctt tggaatcggc aagtgatatt cttcgatgat tttcttgtaa
     9061 taaaacaact caacgtcttt tttcagatag tcaaaaaatg tttcgccagt caaaaatccc
     9121 gactgatgcc attgctccag gttttctttt gttagtaacc gtggccatgc cattgctccg
     9181 ccttgctttt gatttcttcc tcgagggtaa taagggagcc cccttcttga tccactgtat
     9241 acaatcggtt ccttgcctga tggatgatag accagttttc ccttttcgtt ctcgaagcgc
     9301 cctcctcttg atactgtaaa caatccgaca taatcaaaaa aacttaagcc tagacctctt
     9361 aaaaagacat tttctttagg cggtatcttc gaaagatcta catctgcagg attagaagga
     9421 agctgataca gtaaaacatt tttctttgcg tagtctaccc acactctttc ttcttctgtc
     9481 tcatcatttt cccagtgacc gcttgctaaa acaagctgat cgatagaata gctttgctga
     9541 tctgtctgta aaagataacc atcttttctt ttgcttactt tttttactaa agaataaaca
     9601 aactgaatag aagctggaaa ttcttcttgc aactttgaga aaaaccagcg ttgatagagt
     9661 ccgtataaac atcgagtaga ttgctgatct ttttccagac gattgatttc ttctataatt
     9721 agttgcttat ttttggttac cgtctgttcg atataatctg tagcaggtcc tttactccat
     9781 tgaaacaagt tgggtccctg tgttaccaat cctccactgg acaatgtttc atctgtaaaa
     9841 agcgtcactt gttgagaaac agaattcatt aataactccg gtggctgatt caaccgccaa
     9901 actcgacctc caggaccatt gggatcgaaa agaaaaagtt caagagaatg tctgtcctga
     9961 ttattatgga gaatcctttc tgctacactt aatcctctag gtccggcacc aatgatccct
    10021 attttcattt gcatgcctcc atttcgataa attttttcaa taaaacagca tggttctgat
    10081 gttcattttt tgcaccataa acaaaagtaa tgactggatg ttctttcata agttgactga
    10141 gtttaaaaat agcttgcttc ttttcttctt cgaaaatcaa ttcttgctgg tatttctttg
    10201 caaatgaagc aaatcgttct ggtacatggt caaaagtttt gcgtgcttca ttactaggtg
    10261 caatttcttt caaccacaga tctaactgcg ctttttcttt tgaaattcct cgtggccaga
    10321 atctatccac gagtattcta tagccatcct ctttttcagc atcaaggtaa acacgtttta
    10381 atttgatcat agttgtttcc ttctttctac cttttcgctt agcttatata tgtagataat
    10441 agcacaaaat aaaaaaaatg aacggcacaa ctatccattg cgccgttcat gtgatcttgg
    10501 tcaaaatcta atgattgatt cggtttttaa agctgtcata caatcatctg ttttatactt
    10561 ttttactttt gatttttcca gaccatccgt catatccacc cttaagtaaa taaatctctt
    10621 gataacctgc tttacgtaat ttatttgctg tacggatgct caaacttttc ttttgatcgt
    10681 aaatatagac aggctggtct tttcgaatag atcctaatga atctttcaaa acagtgtaag
    10741 ggatgtttct tgcacctaaa atatgcccag catcaaacac atctttttcg cgaacatcga
    10801 ttacttgtgc ttttctcatt ccttctcgaa attcttcttc tgtcaaagtc gttgctgaac
    10861 gtttggccat tactcgtaga tagacttccc aaaggatgat tgcaagcaca atcaatagca
    10921 aaatgatatt aattacccat aaaaccatgt aatgcctccg tttttttaag caaatttcaa
    10981 ggctaatgag gcagcaccaa tgacaccagc ttcattacca agctctgcta atttgatttt
    11041 tgttgattct cttacttgcg ggaaagtaaa ttctttaaaa tatttctcta cacggctacg
    11101 taaaaattct cctgctgccg atactcctcc acctaacacg atacttgacg gatttaatgt
    11161 attccccaag tttccgcaag ctaaacctaa gtagaagcat acgcgatcaa caaccatcaa
    11221 tgcaaacgga tcgtcttctt gagccatttc aaagatatct ttacttgtta cttcttctcc
    11281 gttgtctagc attgctttca gttttgaatc ccctgcgtat tcttctgcca agaaacgacc
    11341 taggcgtacc acacctgtag cgctagatac agtttctaaa catccgcgtt taccgcaagt
    11401 acattcgaac ccttcaggat ccacagtgat atgtccgatt tctcctccag ctccgctcac
    11461 gccgtgaatc aaatttccgc cagcaacgat acctccgcca acacctgttc ctagagtaac
    11521 gaatacaacg tctggatcgt tttctccagc acctttccat cgttcaccta aagcagcaac
    11581 attcgcgtca ttatcgatag cgaatttgat tcctgttcct gcctcgatca gttttttcac
    11641 aaattgaact tctgtccagt ttaagttata cgcaccaata accgttccag cttcgctgtc
    11701 cacgcttcct ggggtaccca tgccgattcc gataaagtct tcggctttca tattatataa
    11761 attcaaacga tgattgattg attcaatgat ttcaggaacg atatgcttac catcatccaa
    11821 aatattggta tcgatgctcc atttttgctg aatctctcct tctggtgtta aaattgcaaa
    11881 ttttgctgtt gttccaccta aatcaatccc aataatcttc ttgtccatta caaatttcct
    11941 ttctttcggc tttcttcaat tcggtgctct ctttttaata tgcgccacgc tgtcatgtat
    12001 gtatcatgat caatcaaacg gctatcatac agattacgca attcgatagt cattaattca
    12061 atgtcataga tgcgtgcacc cacatagata aagatgccaa attttttgaa cagctgctgt
    12121 acatcatata agctctccat ctttaagcac ttccttcata ttatacaagt aaaccatatt
    12181 tttttaaacc cagtaaaata catattacaa aaagaaatac aaaaactagt gtagaaagaa
    12241 tcctttggtg tatcgaatag cttgtctgtt gtttcggtag acccaaaaca ttccctaaca
    12301 ctaatccgcc aattagccca ccgacatgtc cccaaacatc tacagaggta tccagaagac
    12361 caaaaacaaa tgtcaacaca ataaataaaa gaaaatgtct gactagctgc tggatcgctg
    12421 ggttatgttt aaaatgaaac cctaagataa acaacgcacc gaacaaaccg aatatcgaag
    12481 tacttgcacc accagacaga acattcgctt cgttaaaagc aaagcttgct gtatttccca
    12541 ttatcccgct gaacatataa atcaagaaaa aacgccaatg gccatagatt gcctcgattt
    12601 gctgccccat aaaatacagt atcagtgagt tcattacgaa atgcatcagt ccaaaatgga
    12661 tgaagattgg tgtcactaaa cgccaccatt cattgaagtg aacaagataa ggaccataca
    12721 ttccggcata ataagggatc tcaagtcgcg gaaataattc catcatgagg aagaccgctg
    12781 tctgtatccc taaaaaagca taggtccata acggtcgtct taaccatgca cgcaatttta
    12841 tttgctgttg ataattcatg aaaacctccc atttcatttt agtcaataaa caattgttgg
    12901 atcttctgat caaagctctc cggttgccat tgttcattca attgttcacg aaaaacaagg
    12961 ctacatgttt ttttatgaaa attatttaaa taccggtcgt aataaccacc gccaaaaccg
    13021 attcgataac cggctctatt gtagataata cctggtacaa tgaggagatc gatcatctcc
    13081 ggcaaataga gcttctctga ttgtggctca agaacaccaa aactcgaacg tacaagaacg
    13141 gactgttcat ccatttcata aaacgccatc tgacgtttcg gcaaagtttt aggtacaact
    13201 attttctttt gttctttttc tgctcgttgg ataaccggct gtgtatccag ttcaaattca
    13261 ttcgataaag tcgtccctat cacttttgct gacttccagt actcgctggc gaatagctgt
    13321 ttatagattc gctgttcttt ttcctttttc ttgccacttt tggccagatt tttcagcttt
    13381 tcgattcctt tccggcgtaa tcttactttc aattggtcca aaaactcacc cttccttctc
    13441 tcataaaatg gtataggaaa aaaagaaaaa tagcaatcga actttcaaat aaatgcataa
    13501 atagaataaa aagaagacct agaaatgatt ggccttcctt ttttaatgaa tttcaactgt
    13561 tcagcgcgtg tttttgtctg ataaaaagtg catacagaaa atatggcgca ccaaaaaggg
    13621 caatcaaaat tcctgtaggt acttccccac ttagtagaat cactctagca accatatctg
    13681 cactaagcag aaaaatggaa ccgaacaaac cgctcagaac aaaggtccag ttattttctt
    13741 tacgtacgac tagcttcgca gcatgaggag ctagtagtcc tacaaaagat aaactacctg
    13801 tcaccgctac actaccgctt gccaaagcaa cagctaacaa caaaaggatt ttttgacttt
    13861 tagccacgtt aaggcctaat ccttgcgcgc gttcttgacc aaatgatagc aatacaatcc
    13921 tctgtgcata aagtcctcct agcggaatta agataaccgc ccatggaagc aatgcccaaa
    13981 cataaggcca gctcgcaccc cagattgatc ccgctaacca tgcattcaca aatccataat
    14041 tttccttaga taattggatc gtgcatagca atgttaatgc agaaaggccg gcattaacgg
    14101 caataccgga caataagatt cgattcatac ccaaaaagcc atttcgatca taggccgcaa
    14161 gatagacaca gcaagcagct aaaacaccac ctagaaaggc tacaactagc aatagaaacg
    14221 gcagcgaata gtgcgaaaag aaacctaaat aaagcatcac taaaaatcct gctccggcat
    14281 taatgcccaa tatactagaa tctgccaatt catttcttgt tactccttgc aagatataac
    14341 cagaaacgcc aagacaagct cctgctaata aactgatcag taatcgagga actcgaaatt
    14401 ctctaacaat cagcgccaaa gaagatgatt ctttccctat gaacgtggca aaaagctgat
    14461 ccaggtaaat aaaaggcgta ccaatcatta aactacctat agacaggatg acaagtacaa
    14521 aaagcaagaa atagaacgtc caatatattt ttttcaccgt tgttccctcc ttacttgtat
    14581 cagtaaaaac ggaacgccaa tcaatgcaat gactaatcca aaaggtgttt caaaaggggg
    14641 attgatcatc ctcgctgtaa aatccgccaa cataaccaat ccaccgccaa ctaaaaaaga
    14701 gacaggtaga acataacgat agtccgttcc aaccattccg cgaacaactg gcggaaccaa
    14761 cagcccaata aatgaaacag gtccgactaa cgaaacggca atggctgcta acactgcaac
    14821 tagacagaaa acaatcaacc gcgttcgttt aggatgactt ccaagagcta atgcatgtgt
    14881 ttcactcaaa tgaatcaaag atattgcttt tcctaatgaa aaagaaacca aaacagcaag
    14941 tatatataaa ggaaacaata ctgctaattg tttccatgtg atattggcag ctccgcctac
    15001 aaaccaaaaa gcaagatcct gctgcagatt aaagaattgg gtaagtgctt ggctgatagc
    15061 agtaaaaaaa gaactaatag ctacgccagc taaaatcagc tgtaaaggat tcatgcccat
    15121 tttcgaaaac tgcaaaaaac caaatacgag caaagttaca gcaaaagaac ctgccaatga
    15181 cattagaaac acagaaaaat cagaagcttt aggccaaaaa gcaaaactaa gggccattgc
    15241 caacgaagca cccgcgttga ttcctagtaa gcctgaatct gctaaaggat ttcttgtcac
    15301 tccttgcatc aatgcaccgc tcaatgcaaa acaactccct acaagaaatg ctcccgccac
    15361 acgaggcaaa cgaatatttc gtatgatttg gtgactttgt tttgatctat caaaatcaaa
    15421 caacgcctgc caaaaatctg cttttcccac agtcgctgcg ccatttgaaa gagcaacaaa
    15481 aaaaataatc aaaagaacaa aacagaccaa aagcaggagt ctatttttct tcatagtcct
    15541 cataattgat cctcctgctc ttttggcaac aaatcatagg ttagaaaaac tggtttattt
    15601 gttcttggaa gcgtgacaat cgttgcatca atctcaaata tttttttcat acactctttt
    15661 gtgatcactt cttcaggtgt accttctgtg atcaacttac cgtttttcat tgctaataaa
    15721 taatctgaaa aacgtgaagc atgattaatg tcatgaatcg acatcaagat cgttgtttgt
    15781 ttcatttgat tgatttcagt caataactgt aggatctcta attggtgtgc cggatctaaa
    15841 aatgttgtcg gttcatccaa gatcaagata tctgtttcct gagcaagcgc catagcgatc
    15901 cagacacgct gggtttgtcc accagacagc gttgcgatat cccgcatacg aaatccagct
    15961 agacctgtag catccaacgc ccactcgatt gcttcatgat cttgcgtgtc catgccactg
    16021 aacagttttt gataagggta tcgtccgtaa gatactactt cttctactgt catacctgta
    16081 gggtgttcac tagtttgtgc taataatgca attttttttg caagttcttt tgaactgtat
    16141 gtagagatat cagagttatt gacaatgatc tttcctgatt tcacaggtag tatacgggta
    16201 aaacttttca ataaagtaga ttttccactt ccatttggcc caatcaaact agtgattttc
    16261 ccttcaggaa tcgtaacaga catgttttca atgatttttt tgttttcgta gccggctgca
    16321 atgtcagctg ctcttaatct cgtcatttca cccatccttt cgctgaaaat cattctcaat
    16381 tatgacgctg tttcaacatt tcgtcaagcg ctactttcat atctatagcc aaatgaagcg
    16441 gttgacagcg ttcgaaatat caggaataat aaccaatgag aaagattctc aattatgtta
    16501 ggaggaaaga aatgaaatta aaaacaaaag tattcacatt atctagcata ttgctgctgg
    16561 ctggagtact gagcgcttgc tccccctctt cagataaaga aacagctaca actgccaatt
    16621 cgacgacttc atccaatagt actcatacag tcacagatac acttggacat aaagtagaga
    16681 ttccaaacgc gcccaaacgg atcatcggca gttacttaga agactatctg attgcactag
    16741 gggaaaaacc tatcgcccag tggactgttg gcagtggttc cattcaagat tatctgcaag
    16801 atgatttaaa ggatgtccca acgatttctt atgaccttcc ttatgagaaa gtgttaagct
    16861 ttgagcctga tttgctgttg atttcttctt ccgctactgt cgaaggcggg aaatacgaac
    16921 aatatagcaa aatcgcgcca acttatgttg taaaaaacgg aacagatgtc acatgggaag
    16981 aacaactaaa agatgtcggc aaagctttgg caaaagaaaa agaggcagaa aagatcattt
    17041 ccgattatca aaaatccatc aagaaaactc gagaagaact agcagataaa atcgagaaca
    17101 agacagctgc agtgttgtgg gtgaccaaca actcagcctt tatggtcagc aaaaatcggt
    17161 caagtggaag aatcgtatac gacgatctgg aatttggtgt tcctgatcta gtggaagagg
    17221 tctccaaaaa agccacttct gactggtctg ctgtttcctc tgaaaagcta gctgaattag
    17281 atgctgacta catcatcctt gtgaacagtg acaaagatgc tgccatgttc aatgaaccaa
    17341 gctggaagaa tcttaaagca gtgacagaca atcatgtact gcaattcgga cctgaatcta
    17401 gttggctata taacggacct attgccagtc aggaaatggt caatgatatc aaagaaaaac
    17461 taaattgatt ttaactaaaa aaagcatctt cacgattagt cgtgatatgc tttttttctt
    17521 attaacaaac tagacgcata gattttattc ttcttttgaa gcaaccctat tttttactct
    17581 ttcccatttt tgaaaacgat gggcatatag attttcttga tccgttatcc cctcatactc
    17641 ctctataagg gcgaagtctg aaaaatcaat attcccaata tacgtatcgc cttcaaacgt
    17701 gctatcgatc aatgttcgcc agatccgacc agtgtaagga agaagttctt gaaaaatccg
    17761 actaccaccc gaaacataga tatcttctga gatcgtttga ctatacgaca aaagttcatc
    17821 gatcgaatgc attacttccg cattttcttt cgtgcgaaaa tttggctgcg ttgtcaacac
    17881 gatgatatgg cgataaggaa gtgaaagatc ccccattcct tcataggtct ttcttcccat
    17941 cactaaaatt cgatccattg tatgttcgcg aaagaaacgc atatcatttg gcaatcgcca
    18001 tggtaacaaa ccatttttgc cgatcagacc atttttatct tgtgcccaca tggaaataaa
    18061 catattatcc gctccttatg attttagatg ttttgagata aaaaaagtct caattctttg
    18121 accagcaagg aattgagaac tttaaaaaca ttattttgtt tcacggtgca aagtaacttt
    18181 gcgttcgcgt gggcaatatt tttgtttttc caaacgatca ggattgttac gtttgttttt
    18241 gcttgttaga tagttacgtt ctttacaaga agtacactct aaagtgatgt ttacgcgcat
    18301 gtttttccct cccattttta caagaatcct ctaagatgac tctcagactt gattatctta
    18361 ccatgatttt ttctagattg ctagtgtttt tcacgatttt gttaagtaaa actacttgac
    18421 gataaataca acggttttac ttctcaaaca aaatcttttt ctttttgtat aaataccctt
    18481 acacttgttt atatagatta tgttttttaa taaaggggga tactaatatg ccatggaaca
    18541 tgaaggactt tcctgcatca atgaaaaact tagataaatt gacaagaaaa aaagcaatcg
    18601 acatcgcaaa tgcattatta gatgaagggt accctgataa ccgtgcgatc ccaattgcca
    18661 ttgatcaagc aaaaaaatgg tatgaagatg ctagtgaatc agaaaaacga actttcgaaa
    18721 aagaaaaaaa tccaagcaag acggatgaac acgatacgaa tcctcgtgct ggaaaactgc
    18781 ttgattcaga tgtgatcgta gaatatgagg aagaacaatg gatcgtgaaa tctaaagggg
    18841 caaaaaaggc aagcaaccat tttgatacaa aaaaagaagc aatcgaaaaa ggaaagcaag
    18901 tagcacaaaa caaagaatca actttgttca tttataaaaa agatgggaca aaagaaaaag
    18961 aaatcgatta ttgaacatat gtaaaggggt tgtgacgaaa atcgtgtcac aaccccctta
    19021 tgttcgaaac aatggtgttc ctctgccaac tccgaaccat aagtcataat atctataaaa
    19081 cgggaagagc tgttttttga aattgtttct tatcactcag tgttaaacgg taggagaaaa
    19141 cctctttttt attgatattt gattttcatc aaacagtcac atagtcgatt gcttctgctt
    19201 gcggaatcgt ttcatctaca aagtagactt tataccatgt caagaaactg aagattgtcc
    19261 agatttttct ttgctcttct ttacgtcctt cgtgatggtc atctagcaac ttcatgattt
    19321 tttcttgatc aaaaaattct tttgcaaaat ctgcttcgaa caatgctttg acttgttcgt
    19381 agccgtgttc ttctttcaac caagctttga ttggcacagg gaatcctaat ttcacacgat
    19441 tcgaccattt ttccggaaga tgtttgttcg cagcttgtct gaaaatatct tttgtgttgt
    19501 tttgattcaa taaaaactta cttggtattt gttgagccaa tttcatgact tcgatatcca
    19561 ataaaggtac tcggacttcc aaagaacttg ccatagaaag tttatctgcc tttaacaaga
    19621 tatcttttgg catccattga tgaagatcga catattgcat cttattgact tcatctttga
    19681 ttccttctgt tttctcgtaa tgaacagtca tgatttcatc cacagatggt gctgtttgat
    19741 attttggtgt taacacttct gatgcttctg attcttcgaa cactcgagca tgaccgataa
    19801 aatattcttt tgcaggagca agtgattcat ataaatggat ccgtccatgg aaatttttca
    19861 tttttccgat tttacgtcct aaattatagc gtgttccttt tggtaatttt ttcaatcctt
    19921 gagcaatcac acggatgaat tttgaattcg tatggaatcc atacgtttgg tatcccgcaa
    19981 acagttcatc cgccccttct ccagattgga ctacccgcac actttgtgaa gcaagatttg
    20041 ctaaaaagta caaaggtacg caagaaggat ttgaatcagg ttcatctaag tgatactgga
    20101 tcaaagggaa tgctttgaac gacatgtctc cgtcgatcac tgctgctgta ttatctaagt
    20161 ccaatagttc ggttagtttt ctggcttcaa tcgcttcatt ataggtttta tcatcaaacc
    20221 cgattgagaa agaatgatct ggtttcaata cagaggttac ataactggaa tcaaccccgc
    20281 tggataagaa agaaccaact tctacatcac tgaccgtatg tgcttcaata gaagcttgaa
    20341 cggtttcgtc gattttttcg atccattctt gtcgggaatg cgtttctttt gtttcaaaat
    20401 ctgcatccca atattgctga atgtctattt ttccatcttt atacgtataa taatggcctt
    20461 caggcaaacg atagacatct ttgaagaatg tttcgccgtt taatggagaa tattggaaag
    20521 tcatataagg tttcaacgct tctttgttca attctttgtt aaaatcagga tgaggcagaa
    20581 aacttttgat ttctgaacca aacataaatg ttccgttcat ttctgcataa taatatggtt
    20641 tgatcccaaa gtggtctctt gcaccgaata actcattctt ttcattatcc caaatagcaa
    20701 aagcaaacat tccgcggatc ttttgcaata attctgttcc ccattcttcg taaccatgca
    20761 aaaggacttc tgtatctgca tgtgtttgaa aaacgtggcc agctgcaatc aattcttctc
    20821 tcaaaggctg gtaattatag atttcgccat taaaaataat gatctttgtc cgatcttcat
    20881 tgtaaatagg ctgagtaccg ccctctaagt caataatact taatcttctg aatcctaaag
    20941 caacatgttt atcgatatat tctcctgaac tattaggtcc acggtgaacg attcgatcca
    21001 tcattgtatt aataattgtt tttttgttgt ccttgtcgtt aacaaaacct acaataccgc
    21061 acatttaact ttcacatcct aatataaaat tactgtttat ttcattcata tgcatcttat
    21121 catagaataa atagcggacg aatattttct ctcttacaat ttcgtcacgt gaaaacaaaa
    21181 gatgaaagtt tctgttttta cagaaaataa agtcccaata tctaatttac catgttttct
    21241 tttttgaggc taccttattc tcttcgtgtt aaaaaaatat tagtggatgc tatttccaaa
    21301 aaaaccagca aaaaaagaca cgacctaaag atcgtgtcta attatgactg tatcagaaaa
    21361 tatgatacca aacggatcta ttcattaatt aattgacata gtttatctgt tgctctttta
    21421 tgactggata actgaccaat tcatgacgat ccaagtcttc tttattcata tttacagcag
    21481 taatttgact gtcttcatac gtacgataat aataaatccc tttatcaacg ttacagcaag
    21541 aagaataaat cgtatattcg tattttcttt ctccagtatc gcatactcct ttttgttgct
    21601 cgactgagcc tagtatatga aaaaattgac tgatactttc agattctgaa tcacctgatg
    21661 cagcattcat tttagtaaat gtggctttta caaatcgaga aacagaagag agatctccag
    21721 gtaagcccaa tcctcccatc ccacgactat aaacattcaa gctgatctgg tttgaaaaat
    21781 gattttctgg tgttccattt gatagcgagc gataattgtt cagattaaat aattgataat
    21841 caaatggcgg attatttgtc aacacaccga caggattatc atataaatga agtccatctt
    21901 tcatactttc aattactatc gatttttctt tatcagctaa taaccagtgt aaaggtgaca
    21961 agggtagttt ttcactaaaa tcgatattga ctagattgat agtagccaat aattctttag
    22021 cttcgtctat ggttgagcat tgccctagta tccatggaat aaactcaaat ggtgcaacgt
    22081 tatcttttcc ttcttgtaat tctttataat cagcattgcc aggaaaattc agtcctgcca
    22141 tactcaaccc tttttcattt gtagcctcat aatagagtgg atagttggat accccagctg
    22201 ctattcctat cattgcataa tgtttatcta aattttttac ctttctaaaa tcgaaacgat
    22261 aatttcttgg agtaatagtg accacttcat tataagacat ctcataatca aaattccttc
    22321 caaaataatg gtctttcgtt gcataagtga ttgcggtaca catcgtttcc cgtcctaaaa
    22381 caaatcgttt ttacactttt atcttacatc tagtattttt tttatcaaaa aaaggccaaa
    22441 gaagattgat ttactagcat ttataaaaaa tagtactcag ggggaatcaa aaaatattgt
    22501 aggttaaaag tacaacaata aggggctgtg acaaaagtct tgtcacagcc ccttattccg
    22561 aacaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaac gaaaaaatag
    22621 gaaaagctat ttttgtttgg ccgttcttat tacttgatgc aggacagctt cttcacaacc
    22681 tctaatcgtt gatataagga aggtacagga tttgaacctg cgcgccggta ttagccggtt
    22741 cgccggattt cgagtccggt gcattaccac tctgccaacc ttccgtaagt actattttta
    22801 aacttttttc acgaaatgtc aagcctattt ctaatagata aataacagaa atcgcttttt
    22861 ttagcaaaaa agtccattga cagtttgcag ccaacggact taccgatcaa attcatactg
    22921 atagaacact atctgattat ttcaggatta tctaatgcta aaatcgagtc aattgtccgt
    22981 ttgatcagtg caatgatttg ttcttctgtt ggatgcatga tgtttgatga catcaatgac
    23041 gctaagccag tcactgctac ccatgtacca gtatgcagag catcgacata ttcatccgat
    23101 aattgattgt atctttcatc ttctttaact atttcacgga aataattgta tgaaaaatca
    23161 tgcattttct ttccgtcccc atgttcatca atgtacaatg ccatgtaaag actatgatgc
    23221 ttttttgcaa agtagatata gttcaaaccg agatcaatga ttttatcacc agtacgttca
    23281 atagggaaaa catcttcttt tagttgttca aagatagaat ccagcaacgt attttttaaa
    23341 tcttccatgt ttttgaattc cagataaatc ggctgtgtcg aaatccccat ctttttagca
    23401 atatttctcg cagtaaatcc tttaaaccct tcctttgcaa caacatcata agcagctttt
    23461 agaatttgat ccttggtata gacttttctt ctcattaaaa ctcccccact tctttactgc
    23521 agtatatgtt gttttattgt accctatttt tagcattttt tgcaaacatt ttactcaaaa
    23581 cgttatattt taaatttttt ttcagtcttc tttaactttt ttttgcaaaa ttaaaaaaat
    23641 ttggttaaat tcaatgggtt cccttacatt tttagttttg acctaaatga tagttgtcca
    23701 aatctttcat ttcaagttat agttaaagaa aatgaaaaag aaggtgtgta atggaaaacg
    23761 aaactgacgt tatttatatt catcctcaaa aacgaatcgt ttcacagaaa agaaagtatt
    23821 tttatcttgg ttttacaggt gtttttttct ttttattggc ttgctttcta atactcctac
    23881 tgataattgg agtggattgc taactattct tacctcgcca agcaatcttt tgacagatta
    23941 ttttgcttta ggcggtttcg gaagcgcatt cataaatgta gggattttga ccttactttc
    24001 tgtgctttta gcttatcggc acaaggtgat tttaaacggt cccttatttg cttcgattct
    24061 aacagttacc ggtttctctt tttttggcaa aaacctttat aattctatct caatcatttt
    24121 aggtgtgtat ctatacgctg tatttgtcaa taaacctttt tctcagtaca tcatgatcgg
    24181 actgtttggt agcgcattat caccggtcgt tagttatatc acttttggta tgcgttttcc
    24241 tttactgata ggtattttat tggggaattt agctggcata gcaattggtc tacttcttcc
    24301 gcccttagct gctcaaactt tagttttcca tcgtggattc acactttata atattggatt
    24361 cacctctggg ttgattgcga tgacttttac tgctgttttg aggttattca gctattcaat
    24421 cgtcgaaaat acattggttt tcaatgaata tcactttcct ctcatttgga ttatttttgg
    24481 ctttttttcg ctgacagtcg gaattggttt ctattataat tcctttcggc ttttggggat
    24541 tcgagaaatt ttcgattcat ctggaaaatt gacaactgat ttcattgcta attctggtat
    24601 tggtgctact ttgatcaaca taggcttagt tggtctgatg ctgtcttctt atgttttgct
    24661 ggttggcggt cagttaaatg gacctgtcat cggtgctatt ttatctgcgg tgggtttcag
    24721 tgcttttggt tgtcatttaa aaaacagttt tccgatacta gtcggaatat ttattgcttc
    24781 tttgtttgga acttttcatg agatcaccag tactggcatg cttgttgcag ctgtgtttgg
    24841 gacaggtctc gcgcccataa gcggttttta cggcagtttt tacggtgtaa tagctggtgt
    24901 actacatatt gctcttgtcc ataacgtcag cactttgcac ggaggactaa atctatataa
    24961 cagtggtttt tcaactggtt tcgttgcagg tatccttgta ccgatactag ataactttac
    25021 cgctgtcaga aaggaaaaaa agacacttgg aaaaagaatt atcaaaaaga atcatcgatg
    25081 agcttcttac ttatttatat tcccatgaaa taactgaaat acgtattgga gtagaatttg
    25141 ctgaggaagg gcttttcata gagatacagg gtacggcaga aactcagcca gaagatttag
    25201 atgtacttgc tgatttgctg aacaatcctc gggaccctag tatggaaatc tattatcaag
    25261 aactattagg taatccccga catgatcggg aagattatca tctgttaggt agtttgattg
    25321 atgacgtgga aatcatgtat gcccagccga ttatcaatat taagatcttt cgacgtgctt
    25381 ctcattccta ttattaaccc tattttctat ttataaaaga ctgaatcaaa aaagcagctc
    25441 ctgaaagttc gattaaaatc tactttcagg agctgttcaa gcttgacatt attgattttc
    25501 caatccttca tacacttcat ctgttacagg ttctaaaaac tctgaattac cagcagtgat
    25561 cgccaaatgt tcaaaccaac tgtcttttgt cgcaccatgc caatgtttaa tccctttgtg
    25621 cgttacgacg acatctccag ccattaatcg tcttgctggc tttccttctt cctgatacca
    25681 gccttctcct ccggtcacaa gtaagatttg atagccgtct ttatggacat gccagtgatt
    25741 gcgacaacct ggttcaaaag ttacattacc tacaccaaca gaaacttcgg gatcattcac
    25801 taacatattc agatagcttt acccggtaaa tacttgagcg taagcttcgt ttttttcacc
    25861 tgctggaaaa atcacgccat tgtgtatttc ttcgtgtttt gtcgtcattt acgcctcttt
    25921 cttatcttcg ctattttctt ctaaatagat ttcttttgct aaactaaaag ctgaccaagc
    25981 ttttggccat cctacataaa aagctaaatg ggtaatgatt tctgctattt cttcactagt
    26041 aatgccattt tgcttgccaa tcttcagatg agctggcaat tgttcaaaat ttcctcctga
    26101 aattaatgca gatatggtga tgatactacg ctgatgagca gataattctt ctctggacca
    26161 tacttgtcca aataaaacat catcattcaa ttctgcaaat tttggtgcaa aatctcctaa
    26221 ttgatctcgt cctgcggttt gtttttctgc catttctctt attctccctt cacgactgtt
    26281 aggtgattca actatggttc taaggcaact ttattttcta ttgtttcttc gcatttcatt
    26341 ttaaatgtag tctttcagcc tgtccaatgc ttagtttatt cagtcgatat gctttttaag
    26401 catagttgct aacgcttttt tt
//
LOCUS       DAXLPI010000027        25499 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXLPI010000027 DAXLPI010000000
VERSION     DAXLPI010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.027"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            131..3658
                     /locus_tag="ACF3T5_001006"
     CDS             131..3658
                     /locus_tag="ACF3T5_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFD3631630.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="ACF3T5_001007"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="ACF3T5_001007"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFD3631631.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="ACF3T5_001008"
     CDS             7446..7787
                     /locus_tag="ACF3T5_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFD3631632.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="ACF3T5_001009"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="ACF3T5_001009"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFD3631633.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /gene="pheT"
                     /locus_tag="ACF3T5_001010"
     CDS             9144..11564
                     /gene="pheT"
                     /locus_tag="ACF3T5_001010"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFD3631634.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACF3T5_001011"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="ACF3T5_001011"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFD3631635.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="ACF3T5_001012"
     CDS             12998..13735
                     /locus_tag="ACF3T5_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFD3631636.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="ACF3T5_001013"
     CDS             13749..14564
                     /locus_tag="ACF3T5_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFD3631637.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="ACF3T5_001014"
     CDS             14577..15224
                     /locus_tag="ACF3T5_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFD3631638.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="ACF3T5_001015"
     CDS             15240..15896
                     /locus_tag="ACF3T5_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFD3631639.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="ACF3T5_001016"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="ACF3T5_001016"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFD3631640.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="ACF3T5_001017"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="ACF3T5_001017"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFD3631641.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="ACF3T5_001018"
     CDS             18238..18756
                     /locus_tag="ACF3T5_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFD3631642.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /gene="cbpB"
                     /locus_tag="ACF3T5_001019"
     CDS             18863..19363
                     /gene="cbpB"
                     /locus_tag="ACF3T5_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFD3631643.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19643..20563)
                     /locus_tag="ACF3T5_001020"
     CDS             complement(19643..20563)
                     /locus_tag="ACF3T5_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFD3631644.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20689..21741
                     /locus_tag="ACF3T5_001021"
     CDS             20689..21741
                     /locus_tag="ACF3T5_001021"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFD3631645.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACF3T5_001022"
     CDS             complement(21851..22606)
                     /gene="fabI"
                     /locus_tag="ACF3T5_001022"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFD3631646.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22826..23266
                     /gene="fabZ"
                     /locus_tag="ACF3T5_001023"
     CDS             22826..23266
                     /gene="fabZ"
                     /locus_tag="ACF3T5_001023"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFD3631647.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23381..24142)
                     /locus_tag="ACF3T5_001024"
     CDS             complement(23381..24142)
                     /locus_tag="ACF3T5_001024"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFD3631648.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24331..25425
                     /locus_tag="ACF3T5_001025"
     CDS             24331..25425
                     /locus_tag="ACF3T5_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFD3631649.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8447 a         4424 c         5383 g         7245 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgtg cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagcgttta actccaagaa ataagaagga attcacgaaa attgcttttc
    19501 aaatttttgt gaatttcagc ttatttccga aggagttgct tctgcttccg ccgtttatac
    19561 gtgtttagag cgtgggacaa aagtgttctt tacttttgtc ccacgctctt tttagtgaga
    19621 ttcgttgcat cgtttgtcca aattattttc cgacagcaat tggactgttc ggaatcgtga
    19681 aaccagaagc tgtcaattct ttgacatagg agctcaagaa ctcttctttt aaggcatatt
    19741 gttttccgtt caatgcgtag cagaccgtac gaatcgcaaa attgctgttt ccaagatcca
    19801 ctaatccaaa gatagaaggt tcagtttgga gctcttcttg gtatttttca gctaatgtac
    19861 gatttactcg atcaatgatc tcatagattt tgtcatatcc ttcttcagga acaatccgaa
    19921 tatctaaaag tacttgcata tttgcacgag agagattgct gatcgtggtg atgtttcgat
    19981 tcgggatgaa atggacagta ccatccgttg ctttcagctg aagggtacgg ataccaacag
    20041 atacgacggt tccttcaatc gttaagtttg ctagcttgat gtagtcccca acatccatct
    20101 gttgttccat gataataaaa aatccggtaa taacatcact catgaaccct tgggcaccaa
    20161 gaccgattgc cacacctgca attccggcac cagccaaaag tgatccgatc ggcacaccga
    20221 tagcagataa aattgcatag ataaaaaaga aaccaagggt ataatgaaaa atagtattta
    20281 aaagactatg taaagtcttt aagcggctct cgtttagaat ggctttttta gaatactgat
    20341 taaaagactt atcaatcaga tattttccca cacggtgaag aatccaaaaa aggacgatca
    20401 aaaaaatcag atataaactt ttttggataa aggcagccaa aatggcgtcc caatcaatcc
    20461 cgtcccaaaa ccgctgaaac gcatttactt tgctgacagc ttgatctgtc aatgtctcgc
    20521 cagaagagtc tgtcgtaccg gcagttgcta ataatgaaaa cataaaatcc tcttttctaa
    20581 aatttgattc tttctgtatt gtaccaagaa gttttcaaaa aaggaaatag caaaaataac
    20641 tataatgaag gaaaaaagct gaaaataaaa tgacaaataa tatggataat gataaaatgt
    20701 ggagaaaata acgaaggtgg taagtatatg caaatcgatt ggaacaatct aggtttttct
    20761 tatatcaaaa ctccttggcg ttttattgcc aaatggaaag acggacaatg ggaagaagga
    20821 gaattgacag aagataatta cctgacgatt catgaaggct ctccagcgct tcattatggt
    20881 cagcaatgtt ttgaaggtct aaaagcttat cgtcgaaaag acggcaaggt caatctcttt
    20941 cgacctgaac aaaatagtcg acggatgaat caaagtgcta gacgattgct gatgccaatg
    21001 gtccctgaaa agatgttcgt tgatgctgtc aagcaagtgg taaaagcaaa tgaagatttt
    21061 gtgccgccct atggaacagg cgctacatta tacttgcgcc ctttactgat tggggtcggt
    21121 gaaaacattg gtgtccatcc agctccagaa tatttatttg tcgtcttctg tacgccagta
    21181 ggagcgtatt tcaaaggtgg actaaaacca acgaatttta ttgtatctga ttacgatcgt
    21241 gctgctccta aggggactgg tgcagcaaaa gtcggcggaa attacgcagc gagtttactt
    21301 ccaggaacag aagcaaaaga actggattac tcagattgtg tttatcttga tccagccaca
    21361 catacgaaaa tcgaagaagt aggtgcagca aatttctttg gaattacaaa agacggaaaa
    21421 ttcatcactc cgcaatcgcc ctctatctta ccaagtatca cgaaatattc cttgttgcag
    21481 ttagctgaag aacgactagg actgcaagca attgaaggag atattttcat tgatgatcta
    21541 gatcaatttg cagaagctgg tgcatgtggt acagcggctg ttatttctcc aatcggtggg
    21601 atttatcaca ataacaatct tcatgtattt tatagtgaaa cggaagtagg accagtaaca
    21661 aaacggttgt atgatgaatt gacaggtatt caatttggag acatagaagc accagaaggc
    21721 tggattgaag tcgtggaata atttcaaagt aatgagttga aaaaaacgta aaaagaaaaa
    21781 atcaggaaga gactttaaaa agttttttcc tgatttttat atgttttact ttaaagaatg
    21841 caacaaaagt ttatgaaaga tggacacctt tgtcaacgta gataatatca ccgatgattc
    21901 cgctggaaag tgggctgatt aagaaagcac aagcattacc gacttcttcg atcgtcacac
    21961 cttgctgatc aggcgtacga gattcagata actggatcag tttttgatag tctttaacac
    22021 ctgtgacagc taatgttttg attgccccag ctgagattcc attgacccga attcctttag
    22081 gcgagaactc agctgccaaa taacggatag cagcttcaag agaagctttc gcaataccca
    22141 tcatgttata attcgggatt gcgcgttctg aacctagata agtcatgcta acgatcccgc
    22201 tgtttggtgc aagaaacggt tgtgcgtatt ttgatacagc aattaaagag tagctgctga
    22261 tatcttgagc taattgatag ccgtctctgg aaatatcaga cacatttccg gacaattctt
    22321 ctttgttggc gtaagcaact gcatggacaa ggccgtggat ctccccagca tattcaccga
    22381 tggcattcat tgctttttcg atgctttcgt cgctagaaac atcacattct acagtgaatg
    22441 cttcttcgcc gaccaatttg actaatgatt ttttcattcg ttcattttgg taagtataga
    22501 taatttctgc cccttgtttc aacattgctt cagcacaacc ccaagcaatg ctttttttgt
    22561 tagcgacgcc cataatgacg attttttttc cttctagaaa tgacataatg actctccttt
    22621 gtttctgata aaaatttgat agtcgtaaaa aaatagagaa taatagctaa aataacttct
    22681 tgatttctta attattagtc aaaatgtttt gattgtcaaa ctattttttc ttaaattaaa
    22741 aaaagattgc taaatattta ggaatatgat actttgatag tcaaagtaat gaaaatggca
    22801 attgcctgaa agaaggagtt taattatgtc ttacgtatta tctgctactg aagtaatgga
    22861 attgattcct aatcgttatc ctatttgtta tattgattat gtagatactt tagaaccagg
    22921 aaaaaagatc gttgcgacaa aaaatgtaac aatcaatgaa gattttttcc aaggtcactt
    22981 tcctgacaat ccagtgatgc cgggtgcgtt gattctagaa acactggcac aagcaggttc
    23041 gatacttatc ctaaaatcag aagagtttct aggaaaaaga gcttatatcg gtgggatcaa
    23101 taaggccaaa ttccgtcaaa aagttgttcc aggagatgtg ctgaaatgtc attttgaaat
    23161 caccaaaatg aagggatcag tcggtacagc gttatctgca gcatatgtag atgataaaaa
    23221 agtctgtgag tgcgagttca cgttcatcgt caaccaaaat gcttgactat aaaacctgat
    23281 atagcaaaac aaaaggaagc ttacttgctt tttatgtaaa aagaagacgc ctgaatccta
    23341 gcatacgttg tcatctatgc aggattcaga cgttattttt ttatttttgt tttgcttctt
    23401 gcagtatatt gattttatcg atttttactt cttcttttgg tttgtctgaa ctatctgttt
    23461 cgatatttgc aatagcatcc acgacatcca ttccttcaat cacttggccg aagacagtat
    23521 aatggccatc tagtgaagga taaccgccgt ttttataagc gtcgatgatt gcttgaggat
    23581 agtcgtcttt taaaagacca tcatgcatat catctgtatt ttgaacgata aagaactggc
    23641 tgccgttact gttaggatct tgtgagcgag ccatagataa cgcacctctt atattataaa
    23701 ggtggttgtt gatctctgtt tcaaatcctt ctccccaaat gctttcccct ccagtgccat
    23761 cgcctttagg atcgccacct tggatcatga aatctttgat gacgcgatgg aaagtcagtc
    23821 cgttgtagta accgtctttt gcatgtgtca taaagttttc aaccgctttt ggtgcgtgtt
    23881 cgggaaataa tttgattttg attgtgcctt tattcgtcac catttcgacc agatcttcat
    23941 tttcggcgac atctttcgtc aattgtggta attccaatgc gttcaaatcg acgctactgg
    24001 cagtcgtgct ttccgcagta gatgtacttt ttgaactttc tgtagacgaa ctagaagaag
    24061 tcgtgttgtt tgcagaacaa ccagcagcaa acagcaatgc tgctgctaaa ctaatactta
    24121 aaataacttt atttttcttc ataactgttc ctttccaatt tactattttg ttatctttaa
    24181 tgataacaaa atagagggaa aatacctact taaattattg gaatttatca tttattttaa
    24241 aagaaaaaga aaaggtgtag accatttact ttgtgtttgc tataatgtaa gtggatacaa
    24301 aaacgaaaag atgcaggagg aaatggtaat atgggaaaat taggaatctc gatttatcca
    24361 gaacggtcaa catttgaaaa ggacaaagcg tacttggatt tagctcataa gtatgggtat
    24421 aaacgggttt ttaccagttt gctgcagatc aatgatgata aagaaaaagt tctttcagaa
    24481 tttaaagaag tagttgatta cgcgaatagc ctaggcatgg aagtaatggt tgatatcaat
    24541 ccagcgttat ttgaacagtt ggagatttct tacgatgact tatctttttt ccataagatg
    24601 ggtgcctacg gggtccgctt agatatcgga ttcacaggag cagaagaagc aaaaatgacg
    24661 cgcaatcctt ttggtatcaa aatcgagatc aatatgagtt cgggcacgaa ttacgttgac
    24721 aacatcatga gttattcgcc aaacacggat aatttattag gttctcataa tttttatcct
    24781 catcgatact ctggtttagg atacgaacac tttgtttttt gttcagaaaa attcagaaaa
    24841 tataatttga atacgatggc attcgtcaat tcgcaatctg ctgagtttgg cccttggcct
    24901 acgcaagatg gcttgtgcac gctggaagac caccgagatc ttgaaattgc gacacaagtg
    24961 aagcatttaa tccttacagg attgattgat gatatttcta ttggaaatgc gtacgcaagt
    25021 gaagaagaat tgaaagaaat ggcagaagca tttaacgcgg attatccaac attgaaagta
    25081 gatacggaag aaggtattac agaaaacgaa cgaatctgct tgtttgataa ccttcatagt
    25141 tatcgagggg atcgctcaga atatatcctt cgttcaacga tgactcgtgt atactacaaa
    25201 gataaagatt tcccgcctca taatacgcga gatatgcatc atggggatgt actgattgac
    25261 aatgaagggt acggtcaata caaaggagaa acacaaatcg ccttgaagga tatgaaaaac
    25321 gacggacgtg tcaatgttgt aggaagaatc tccgatgacg aattattcct ccttgacttt
    25381 ttgaaaccat ggtcaagctt taaattaatt gaaaataaaa aatagataat tagttattgc
    25441 agaaataatc cgctagaaat agccagttat ttctgctttt ttattttgtt tttttatag
//
LOCUS       DAXLPI010000028        25444 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXLPI010000028 DAXLPI010000000
VERSION     DAXLPI010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.028"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            35..2095
                     /locus_tag="ACF3T5_001026"
     CDS             35..2095
                     /locus_tag="ACF3T5_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFD3631650.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2178..2747
                     /locus_tag="ACF3T5_001027"
     CDS             2178..2747
                     /locus_tag="ACF3T5_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3631651.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2922..3728)
                     /locus_tag="ACF3T5_001028"
     CDS             complement(2922..3728)
                     /locus_tag="ACF3T5_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631652.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3867..5381
                     /locus_tag="ACF3T5_001029"
     CDS             3867..5381
                     /locus_tag="ACF3T5_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFD3631653.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5784..6767)
                     /locus_tag="ACF3T5_001030"
     CDS             complement(5784..6767)
                     /locus_tag="ACF3T5_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFD3631654.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6772..7419)
                     /locus_tag="ACF3T5_001031"
     CDS             complement(6772..7419)
                     /locus_tag="ACF3T5_001031"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFD3631655.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7504..8136)
                     /locus_tag="ACF3T5_001032"
     CDS             complement(7504..8136)
                     /locus_tag="ACF3T5_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFD3631656.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8152..8703)
                     /gene="coaC"
                     /locus_tag="ACF3T5_001033"
     CDS             complement(8152..8703)
                     /gene="coaC"
                     /locus_tag="ACF3T5_001033"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFD3631657.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8700..9437)
                     /locus_tag="ACF3T5_001034"
     CDS             complement(8700..9437)
                     /locus_tag="ACF3T5_001034"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFD3631658.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9434..10423)
                     /locus_tag="ACF3T5_001035"
     CDS             complement(9434..10423)
                     /locus_tag="ACF3T5_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFD3631659.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10681..11223
                     /locus_tag="ACF3T5_001036"
     CDS             10681..11223
                     /locus_tag="ACF3T5_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFD3631660.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11240..12325
                     /locus_tag="ACF3T5_001037"
     CDS             11240..12325
                     /locus_tag="ACF3T5_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631661.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12337..13143
                     /locus_tag="ACF3T5_001038"
     CDS             12337..13143
                     /locus_tag="ACF3T5_001038"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631662.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13144..13971
                     /locus_tag="ACF3T5_001039"
     CDS             13144..13971
                     /locus_tag="ACF3T5_001039"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631663.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            13968..15041
                     /locus_tag="ACF3T5_001040"
     CDS             13968..15041
                     /locus_tag="ACF3T5_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3631664.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15469..18165
                     /locus_tag="ACF3T5_001041"
     CDS             15469..18165
                     /locus_tag="ACF3T5_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFD3631665.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18230..18730
                     /locus_tag="ACF3T5_001042"
     CDS             18230..18730
                     /locus_tag="ACF3T5_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3631666.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            18742..19236
                     /locus_tag="ACF3T5_001043"
     CDS             18742..19236
                     /locus_tag="ACF3T5_001043"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3631667.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19257..20066
                     /locus_tag="ACF3T5_001044"
     CDS             19257..20066
                     /locus_tag="ACF3T5_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFD3631668.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20063..20884
                     /locus_tag="ACF3T5_001045"
     CDS             20063..20884
                     /locus_tag="ACF3T5_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFD3631669.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20935..23034
                     /locus_tag="ACF3T5_001046"
     CDS             20935..23034
                     /locus_tag="ACF3T5_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFD3631670.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23031..24149
                     /locus_tag="ACF3T5_001047"
     CDS             23031..24149
                     /locus_tag="ACF3T5_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFD3631671.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24188..25066
                     /locus_tag="ACF3T5_001048"
     CDS             24188..25066
                     /locus_tag="ACF3T5_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFD3631672.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25212..25409
                     /locus_tag="ACF3T5_001049"
     CDS             25212..25409
                     /locus_tag="ACF3T5_001049"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFD3631673.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8028 a         4435 c         5404 g         7577 t
ORIGIN      
        1 atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac gttcaaaaca
       61 actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa aacactacgt
      121 taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata tgcatgtacc
      181 cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg agaccactga
      241 ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc tacttcctgc
      301 attgacttcc tataataaag tcagttatcc tccaacgata aaccactact ggttaattcc
      361 taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta tcttttccga
      421 tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt tgaatcatgc
      481 tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg aaaaagatta
      541 ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt cgatatctga
      601 tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca aagtaatttc
      661 aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt ccttgtccta
      721 ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg ttcgtgctga
      781 tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt acgcccctaa
      841 agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag aagaaatcaa
      901 aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat taacttatat
      961 agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag aagcatctgc
     1021 tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag aacttcaagc
     1081 agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg ctgaattttc
     1141 tgatcgagtg ccaaatatag atttatcgac ttatgaacaa aatgggaaag tcacttctat
     1201 ttctgattgg atgcaagcaa acggattaga cagaaaaagt cactggtcgg agatccttga
     1261 agtacttcac tcagaactga tgaataatag gaagcaatca ggaggggaaa ttacctttac
     1321 ttatgtttat ataccagaca atcgttcatt agaaggtcat gatcttattg ttcatccaaa
     1381 cgaaaaaata aacaaagaag agttgatcca atcagcaaca aattccttag gtaaacccgt
     1441 accgttaaag gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg gttatttgac
     1501 caaacaaaca ggtgatttta cgattatgta tagttatcat gacccgtatt cactccaaga
     1561 actccatgct cagtcactat tacatgtttt accattaaac aaacagatag atcaaaaaga
     1621 acaaaaaaga caagaagaaa gcttgtcaga aaaaaaacaa caaatggccc atgcagtttc
     1681 tccaacaggg aaagtaaaac aaacaagaag cctagagtta gcaagaatat ctagaaaaat
     1741 ctcagaatct aataaaacga taaggcaaga agctcatttg gccccacaag tttctggatt
     1801 ggaaaatgaa attttagagc ctaaaataaa acaacaatca gaaaaagaat ttgttgctaa
     1861 acagtcggaa gatcagaagg aatcagatga ccagaaggaa acgaagaaaa aacgcttgtc
     1921 aactattgat tccaccgatg agcatctgca gccgcaagta ccacttccta gtccgggaac
     1981 acccgcacct ggtggaggta ttccttcttc tgctttagga gatttcatgc gaggtatggc
     2041 aggaacatgg gtatacgcta atcgaaatga atttgacgca gatgactggt tctgatttat
     2101 atctctatct gttatagtca aataagtagc tgagtcatat gttatttggc ataatggaga
     2161 cgaggaggga tagaaaaatg ttagataaaa cgttacctta cgcagaaatc tggatgacaa
     2221 gaccacttca acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa tttcgatttt
     2281 atcaaaaaga agatgaatct gcttgggcgg agattgagac ttccgttggg gaatttcaaa
     2341 cgacagatga agcacttgag tattttcaac aaaactttgc tccttatcct gaagaacttg
     2401 aaagaaggat gttttttgta gagacaaaaa aaggagaaaa agtagcaacc tgtaccgctt
     2461 ggcgaaaaaa atcaagagag caagtgtatc cggtttttca ttggttagca gtgaaaccag
     2521 attaccaagg aaaagggctc gcaaaagcat tgactgccag agtcttgcaa gaatttccta
     2581 ccctccatac ggacggacca atctatcttc acacacagac atggagccat caggcgattg
     2641 ctctttataa aaaaatgggt tttactttta ttgctgaaaa tctagacggc acacctaatc
     2701 cagattatga aaaagttatg caggttttaa gtaaattaga taaataacga aatggtgaaa
     2761 taagtcctca gtattgggat aaaaaacgaa atagtcattc ctcatatctt acttttttga
     2821 gttaatacta tcggataact aatggcatgc catacattca aaaaagaagg ggcagtgaca
     2881 aaagtattgt cactgcccct tctcttgttt tttagcatgt tttatcgttt gattttttta
     2941 ggaataaaat tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata gaggtaacca
     3001 aatacactga aaacaactgc tccaataaaa ttaacaaata aatccttcat agtatccatg
     3061 attcccacgt ctaagtaacc attttggatg attgttttat ctgttttacc atctgcattt
     3121 tgactttcaa tgattgtccg agtgatatct ggaacatgat agacagtgtt tccgtcttta
     3181 cttaactcca cactattgat ttgctggatg atcgtatctt tttgcatatc gaattgcagt
     3241 aatttatctg ctgaaaattc cacaaactcc cacataacac caactgtcat cgaaaaacag
     3301 aaggaaacaa ctgctagtaa taaaggagtc aagttcatgg tttttgtatt tttattgagc
     3361 aaataaacaa gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc atgtagaaaa
     3421 gtatcccaca ttgaaaaata gccataaaaa tcacttaact ctcctaaaac tgttgaacta
     3481 aaaataaaag ataacaaaat gatttctagc atatttggaa tctgtatctt taatatcttt
     3541 tccagaaaat aagggatttc aaataaaatt aatgtcagta ataaaagcat tgtatttccc
     3601 cagtgttggt tccagacctg atccactagc ataataacga cgatgatacg taataaaata
     3661 tagattccgt ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa
     3721 tgattcataa aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac
     3781 caaagctata tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata
     3841 gtataaggag aaaagggggg atacaaatga aaaaattagt tgctggttta ttaacagccg
     3901 ttgcacttct tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt
     3961 ctagtacgaa ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc
     4021 caaaagaaat gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg
     4081 cagcagctat cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg
     4141 ttgccggagg aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc
     4201 aaaaagaaga aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg
     4261 gtggaggaac gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg
     4321 tcgattggct agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga
     4381 gtgaaaagag gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg
     4441 ggttattgaa taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag
     4501 acattaagaa aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac
     4561 caaaagaggt caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag
     4621 aatttattga aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat
     4681 caactggaga tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata
     4741 aaatacaaat tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc
     4801 gcggagaagg agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata
     4861 caagggataa agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt
     4921 ttgaccaagg cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg
     4981 tcacaactgg aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac
     5041 tagcaaaaac agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca
     5101 accgtgccac agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg
     5161 caccaggaat ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag
     5221 ataaagaaaa ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac
     5281 atggaaacaa ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag
     5341 cgggcataca agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa
     5401 gaggttgtga aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta
     5461 gtgaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacagcca agtaaaaaca
     5521 gcttcttatg tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg
     5581 tttattcgga aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac
     5641 acgtataaac ggcggaagca gaagaaattc cttcggaaat aagctgaaat tcacaaaaat
     5701 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
     5761 cctctttttt gatcagcatg attttattca gttatcttca aaatgatttt tccgatgttt
     5821 ttgtttgctt ccatatattc atgcgcttgt ttgactgcgc tgaattggaa tgtacggtca
     5881 atgattggcc gaatcttgcc attttctaga aaaggcccag ctttttgcat aaaagtttgt
     5941 gcgagctctg ctttatactg atcacttcta ggtgtcaata atgtaccaag cagttggaca
     6001 cgtttcccaa tcaatacacc aaggtctatc tcaggcacga tggtcccacc tagcatacca
     6061 atcaagaccc aacgtccatc aacggctata gaagctaaat tcttttccca gtaagaagcg
     6121 ccgatgaaat ccaaaataac atcgactccc cgaccttcag tgatttttcg tacctcttct
     6181 tgaaagtctt gttttttata gttgatcaat tcatctgcac caagttcttt acaaaaggcg
     6241 agtttttctt ctgatcctgc agtaacgatg attttcgcgt gagaaagctg tctagctaat
     6301 tggatcgctg ctgttcccac accacttgct cctgcatgga tcaagacagt ttcgccttct
     6361 tgtaattttc caagccagta aagtgtttga taagcagtca aaaaaacttc tgagattcca
     6421 gcagcagaaa catagtctag cgtatcaggg agaaggattg cacgatcggc aggcatcaca
     6481 gcatattcgg cataaccacc tagattgact aatccgtata cttttgtccc aggagtaaaa
     6541 gtggagatat cacttttatt ctcaactacc tctccagcta cttcgactcc caaaataggg
     6601 tagggaggtt tcaaagagac tgattctctt ttcataatat ctgttcgatt gacagctgct
     6661 gtatggactt tgatcagtaa ttcgccttgt tttggtttag gaacaggcac ttcaatttgt
     6721 tctaatacat ctacagaacc aggatgcttg attgaaatgg cacgcatgga tctattcctc
     6781 ctttgcccat tcttggaatg ctgtattcaa ctctttgcta ccttcagaaa gtactagagt
     6841 cgtacgagaa atatccgttt cttccactag agcggcaagt acagcagcaa ctgtagagcg
     6901 agtgatcgtt tttgatgcct tctcattagg cttagttaat tgaatggaag taacttcttc
     6961 atcgttagtc aatgtgactg gttgaagaat gacaaaatct aagttagttc gtttcagcca
     7021 ttcatctgca taatgtttag taatataata atctgtcatg gactcaggcc attttgctgg
     7081 ttcatctgca taaacagcac tgaccatcag atagcgagag acatgcgcat tttcagctgc
     7141 gatcactgtt ttgattgcgc catccaagtc tacttgcaga agattctttc cttgagagcc
     7201 ggcagcaaaa atcaatacat cgatgccttt gaatgcttcg gtcatttttt ccacggacca
     7261 agttaaatca aaagagacat acgaaacatt ctcttttttt actaatgttt gggaagcaac
     7321 atctctcacc cctgcaaaaa tttcatggga acttggagct aaatctttta tcaggtgtcg
     7381 gccaatttgt ccgtttgcac ctacaacaaa aattttcatt tggatcatcc tttcgacgtc
     7441 aaatctttcc attcaatagt aacaccaaaa gagaaaaaaa tcagagatcg gctacgatga
     7501 ctatcagtta gtaagagaag ggtgcttgaa gtatcttaac aagacattgc atactgctgc
     7561 agtcgcaaga cctgcgacga ttccttcagg tataccactt gtaaaaataa ccgctagaat
     7621 agctgaataa acagcattta aatttgttcc gatgactttg gcataatctt gctggaataa
     7681 aaagtagatc atattcatca ctaaaatcgt attggtcaat gaaccggaaa gtccagctaa
     7741 gaataaagaa acggaatctg ctttttgttt cattagtttg cgcagacctt tataaacgaa
     7801 ataaggaacc accccaatca agatgcgagg aataaacgcg atcagcaagg ctttccagct
     7861 tccatgttct gttccgatga ctgggataaa cggagaaaag acaaaagaca aaggtgtttg
     7921 gataactgtg ctacggatca tgctgatcag tccaaatgta ccgccaagaa aagcacctag
     7981 tcttggtcct aaaacgatcg aagcaatgat tacaggaata tgcatggttg ttgcattgat
     8041 tggaccgata gggataaaac ctaaaggcgt aacagctaat aaaatcaaaa tagctaaaaa
     8101 catcgcagtt aatgtaaagt tttttgtatt tttcataagt actccttttt tctaagcatt
     8161 tctttcatta agagtcagca tgacctgttc gatgatcgtc tgaatatcag ctaaggcacc
     8221 ttttccaaaa tctccacaag ctaaaagaga ttctcttggt tcaatttcct gatagccgac
     8281 agatctcaat gtttccagat tacgctgagt gatcgggttt tgatacatat tcgtgttcat
     8341 ggcaggggca atcaattttg gtgttcccac tggaagcgcc attgcgactg ttgaaagaag
     8401 atcgtctgca atgccgtttg ctagcttgcc gataatgttt gctgtagcag gcgcgatgag
     8461 aaacaagtca gcttgttttg ctagttcgat atgattgatt acctgcggat ttgattcttg
     8521 catgacgtct gtatggactg cgcgcttcga cagagattgc aaggttaatg gcgtgataaa
     8581 atgtgtgctg cttttagaca taatcacatc tacctgggca cctaatttga cgaattgact
     8641 tgtaagatca gcgcttttgt aagcagaaat acttccagta acgcctagta agatcttttt
     8701 cattttgatt cctccagtct aattttctca gtaatcaatg cagcaatctc agatttactt
     8761 tgagcttctt ccactgttcc atctttagat aagagatagc cgtggtgtgt ctcgccatgg
     8821 atttccgtca aatcatttgc gaaaataaaa tcagttttgt tatttatcag actatttttt
     8881 gcgactgcca gaagagattc ctttggtaca tctactaata gtttgaaacc aatgattttt
     8941 gcttttggct gccattcacg tagataagaa atgatttttg gtgttttctt caaaacgagc
     9001 acaagatgct cggtgttaga agagatcttt gaaacagtgg tatctgtctg ttcattttcc
     9061 gcaaaccaat tgtccagttc ttcttgcgtt gaagaagtgg gtaggttttt tgccagctgt
     9121 tcttctgaaa aactaaaagc aggagtaaaa tcactgactg ccatactgtg gataatcgca
     9181 tcgtagtgtt cttttttcgt tagttcttct aattgtaaaa ataagtccag tgcagattcg
     9241 atcgtataaa tcgttatatt ggagtgtctt ttcggtttca gtgcttgttt cgtagtcacg
     9301 taatcgattg tggtatccgg attggcagct aaataatcag ctaatgcttg ccctaatcgt
     9361 cctgtcgaat gatttgtaat agcacgtact tgatcgattt tttcagtcgt tcctccagct
     9421 gtgattagaa tcttcattta tggtcaccta catttactag tgttcttcct tggtgtgttc
     9481 cagcaagtag tttagatgct gtttcaggca attgatccag agtaatctct tggacgacca
     9541 gttcatctgc aatctgcaaa tccgccatct gttgccataa agaaagacgt tgatctattg
     9601 gtacattgac agaatcaatt ccaatcaact ggatgtttcg cagaataaaa ggcaagacag
     9661 tcgcgtttag tttgattccc ccagcattgc cacataaagc tactgcaccg ttataagaga
     9721 tcaatggaag aagactgctt agctgctcgc cacctactgt gtcgatgaca tagtcgattt
     9781 gctgttttcc taaagctttt gttgtctctg gcagaaattc atcaggtcgt acgacttgca
     9841 aagcacctag actttttagc catgtgacag actcttcttt tctggaaaag gcaatgattg
     9901 actggaatcc tagtttatgt aacagggcta tcgctgtact gcctacaccg ccagaagcac
     9961 cagtaacgac aactttttta tcggaggtca taccttgacg gattaaagcg ttcacacaca
    10021 acatagcagt aaaccctgct gtaccaagga tcatcgcttg acgcagcgtc atattttttg
    10081 gtagaggaac taaccagtct cctggtactt tttgatattg gctataaccg cctggatggg
    10141 tgacgccaag cccaaaacca gtagcaagta caagatcttc ttttgaaaaa cgtggattgt
    10201 tactttggac gacagttccg gataaatcaa tccctggagt catcggatat gttcgtatca
    10261 cctgaccaga ctcgctcatt gccaatgcat ctttataatt gacatcagaa taggcaattt
    10321 ttaccaagac ttcattttca gccaactcag taaatggcag atcactgatt gctgtatgga
    10381 aaacaggttc ctttattacc tgaaaagatc gaaaagcgtt cattcgtgag acactccttt
    10441 taaaattttc ttttatcata ccataaagcc tactctttta ttcttgttct tccgtaaatt
    10501 tagacaaaaa ttaggtgaaa aataaagaat caaacaacta gaatttattc caaaagataa
    10561 agaaatattc agcaaaaaaa attttacaaa tggttgcctt tttcaataaa tttcgtatac
    10621 taatacgagt ggtagtaaac aggaagttta ctattagaaa acttcgattc ggaggaaatt
    10681 atggaaattg gtgagaagtt aagaaatttg cgcatccaaa agaatttgac acaagaagaa
    10741 ctaggcgaac gcacagattt gtcaaaaggg tatatttccc agttggaacg agatttaagt
    10801 tcaccatcaa tggaaacatt cttttctatt cttgaagtct taggtgtgac tccagaagaa
    10861 ttttttcatc aagaagcagc gaatctgcaa gtggtttatt caaaagaaga tcatacggtt
    10921 tatttcgatg aagaaaacgg gtatgaattg gaatggctcg tggctgattc gaatgaaaaa
    10981 gaaatggagc ctgtcttgct gacttttgaa ggtgcaggag aatataagac gtttgaacct
    11041 tcattatctg aaacgtttat ttttgtatta gaaggggaat tagagttaag cttaggagag
    11101 acggtctatg tggcaaaagc aggacagtcg atctattatc aagcgacaaa acaacatcaa
    11161 ttaaggaatc atctcaaaaa acgaacaaaa gtattgattg ttgcaacgca atcatatttg
    11221 taatataggg ggtaaaaacg tggggaaacc aatcatttca tttgatcaag tagtgaaacg
    11281 ctatgatgat gaaacagtat taaaaaaagt aagttttgag atcgaacaag gaaaatttta
    11341 tacactgctt ggtccttccg gctgtggtaa aacaacgatc ctacgtatta tagctggatt
    11401 caccgaagct acagaagggg acatctattt tgaagggaaa cggttgaatg atttgccagc
    11461 taataagcga caagtaaata cagtcttcca agattatgca ctttttcctc atatgaatgt
    11521 ttttgataac gttgcttttg gattgaagat caagaaaatg tccaaatcag atatcgaaaa
    11581 gaaggtaaaa gatgctttgc gtatggtcca gcttcctgga tacgaaaaac gggagattag
    11641 tgaaatgtct ggtggtcaaa gacagcgtgt agcgattgca cgagcaattg taaatgaacc
    11701 gaaagtattg ctattagatg aaccgttatc ggcgcttgat ttgaaactac gtacagatat
    11761 gcagtatgaa ctgcgggatc tgcaacaacg attaggaatc acgtttattt ttgttactca
    11821 tgaccaagaa gaagcgttgg caatgagtga tgagattttt gtcatgaata aagggaaaat
    11881 cgtccaaagt ggtacacccg tagatattta tgatgagcca atcaatcatt tcgttgctga
    11941 ttttgttggg gagagcaata tcgtggacgg tgtaatgatc gaagataatc tagttgagtt
    12001 tgttggtaag caatttgaat gtgtcgatgg cgggatgcgt ctaaatgaac cagttgaagt
    12061 tgttttacgc ccagaggatt tgacgatcac gacaccagat aaaggaaaac tagtcgtaac
    12121 ggtcgatact cagctgtttc gtggtgtcca ttatgaaatc atctgttatg atgaacagca
    12181 aaatgaatgg atggtccatt caacgaaaaa agctaaagaa ggaagcaaag taggattggc
    12241 ctttgagcca gaagatatcc atgtcatgcg tttcaacgag tctgaagaag aattcgatgc
    12301 acgtcttgac agctacgaag aatagggggg acaagaatga aaacaatgcg caggatctat
    12361 tccgtccctt atattttgtg gctcggttta ttcgtgatcg cgcctgtatt aatgatcatt
    12421 tatcaatctt tttttgatat gaatgggcaa ttcacgctga ataattatct atcttatttt
    12481 acgtcgggga catcgctttc tatgacgatc aattctgtat ggtatgcatt tttgatcacg
    12541 tttttcacgc tgatcatcag ttacccgact gcttattttt taacaaaact gaagcacaag
    12601 caattatggc tgatgctgat tattttgcct acatgggtga atctgctgct taaagcttac
    12661 gcatttattg ggattttcag tgtgcacggc agcgtgaatc atttctttga ttttatcggg
    12721 atcgggccga aacagatatt attcacagac ttcagttttc tgttcgtggc aacgtatatc
    12781 gagattccat ttatgattat gccaattttc aatgcattgg aagagatgaa tccttctttg
    12841 atcagtgcta gtagagattt aggcgcgaat agctttgaga ccttccgacg agtgattttt
    12901 ccgttatcat taaatggtgt gaaaagtggt gtccaagcag tatttattcc ttctctttct
    12961 ttattcatgc tgactcgtct gattggcgga aatcgggtga tcactttagg aacggctatc
    13021 gaacagcatt tcttggttac acaaaactgg gggatgggtt ctacaatcgg tgtgatttta
    13081 atcattgcta tgttcatcgt gatgctcctg acaggcgaga agaaaaaagg aggtcgctca
    13141 taaatgaaaa aatttaaatg gtcgtatctt tatttaggga tcgtcttttt attcttatat
    13201 gcaccgatct tctatctgat tttttattcc ttcaatgaag gaggaacgat gaacagcttt
    13261 acaggattta cttgggaaaa ttatgaagct gtttttgaag atacacgttt gatcattatc
    13321 gtattgaata cgttcatgct ggctttctta tctgcattga tcgccacgat catcgggaca
    13381 tttggggcta tgggaatcta ttacacgaaa aaacgacgtg cacggacagc gttgttgagc
    13441 atgaataaca tcttattagt ctctcctgat gttattatcg gtgctagttt cttgattttc
    13501 tttacactac taggattcgg attaggtttt cagagtgtat tgctttccca tattgctttc
    13561 agcattccaa tcgtcgtctt gatggtcttg ccaaaattgc aggaaatgaa cgattcaatg
    13621 gtcgatgcgg ctagagacct tggtgctaat aatttccaag tagtcaaaaa tatcattttg
    13681 ccttatcttt ctccaggaat catagctgga tattttatgg catttaccta ttctcttgat
    13741 gattttgcgg ttacattttt cgtcacagga aatggcttta cgacgctctc agtagagatc
    13801 tattctcgtg caagacaagg aatcagttta gaaatcaatg cgctaagtgc gttagttttc
    13861 ttgttctcca tgatattggt cattggatac tactttatta gtaaagaaaa ccgtccaaaa
    13921 cggctgaaga aaaacagaag agtacgcgag ggggtggcac ggctgcgatg aaaaaattac
    13981 aatctctgtt tttaggtatt ctcatcatta ttgcggtgct gctgcttggt gttcgtcaac
    14041 ttgaaaaaac aagtggcatg agtggagcaa aggtacttac catctataac tggggagatt
    14101 atatcgatcc tagtttgatc acgaaatttg aaaaagaata tggatacaaa gtgaattatg
    14161 aaacatttga ttctaatgaa gcgatgttca caaaaatcca gcaaggtggg acaaactacg
    14221 acatcgctat accaagtgaa tatatgatcc aaaaaatgat caaagaaaaa ctagtacttc
    14281 cattagatca ttcgaagatc aaaggattaa ataatattga tccacgattt ttagacttgg
    14341 attttgatcc agataatcag tattcgattc catatttttg gggaacttta gggatcgtat
    14401 ataacgataa attctttgat gcaaatcaaa ttaagcattg ggatgatttg tggaagccgg
    14461 aattaaaaga cagtttgatg ctgattgacg gtgcacgaga agttatgggg ctttctttga
    14521 atagtttagg ctattctttg aatagtaaga atatggcaca gctgacagaa gcagcaaata
    14581 aattgaatca tttaacacca aatgtcaaag caatcgtggc agatgagatc aaaatgtata
    14641 tgatcaatga agaagcctcc gtggcagtga cattttctgg cgaagctgcc gatatgatgt
    14701 gggaaaatga acatttacat tatgtgatcc caccagaagg atccaatctt tggtttgata
    14761 atatcgttat gccaaaaacc gtgaaaaata aagaaggtgc ctatgatttc atcaacttta
    14821 tgctagaacc ggaaaatgcg gcacaaaatg cagagtatat tggttattct acaccaaatg
    14881 aagaagcaaa agcgttattg ccaaaagaca tttcgtcgga cgaacaattc tatccatctg
    14941 atgacacgat ctcccatctt gaagtctaca aagatctagg atcgaaatac ctaggcatct
    15001 ataatgacct attcttagaa ttcaagatgt ataggagata atggcaaaac tcagtaagaa
    15061 tggggactta tcaacgttct aaagagctaa aaatcgtcct ttttctatct gagttttcca
    15121 atcttctacc aaaagcggat gaaacgccgc tttttttgtg ttattttatt ttttctacca
    15181 cgtttttcta ccagagaaaa gtggacaaaa ccattgtaaa atagggcccg agacgggcta
    15241 gtaaatctaa gattgaaaaa gttacagaag gataaagtaa ttggaaacta tgcaaatata
    15301 cttaaccctt ctgtaatcta ttaataaaac taatatatga atccagttca atatttgtag
    15361 ctatgaaaaa acataaaaat catattagat aaaaactaaa ttggtagata aattattttg
    15421 tcagatatct gaggcaaata ttcgactatt tgtgtttcaa agtaaaaatt ggaatacaaa
    15481 tatagaatag acatattgca gccgatagtt tattatgctt ttagacacat taaactggag
    15541 gaaaaaatga aaaagagcgc taaagatcga gtttttgatg agctgatacg actaacaggg
    15601 aatatgtctt ctgtcactgc cgagaaaatt tcgaagaatc taggtatctc tcgtcagaat
    15661 gccagccatt atttgacgag attagtggaa gataaaaagg tcgagaaatt accgggaaag
    15721 cctgttttgt ggaagccttt agatgaatat gcggttgtag ataatacgga gcaaataaac
    15781 gaagcctttc attctgtagt tggtcatgac ggaagtctga gagaggtcat tcaaaaatgt
    15841 atagccgctg tcaaatatcc gcctaacgga ctcagtgtat taataaatgg agccacgggt
    15901 gttggaaaaa gttttttagc cacaaaaatt tttgaatacg ctatccatga gcaaattatt
    15961 gaaaaagatg caccatttgc tatcttaaac tgtgccgatt atgcggacaa tcccgaactg
    16021 ctttcggcaa cactgtttgg atacaagaag ggggccttta cgggggctga aaaggatacc
    16081 gatggtttat tggcaacggc caacaacggc tatctttttc tcgatgaggt ccatcgccta
    16141 tcgaaagaaa atcaagaaaa gctctttcta tttattgata ctggaaacta tcgtccagtc
    16201 ggtgaaaatg taaattggca ttctgctaag gttcgtttta ttttcgccac gacggaaaaa
    16261 ggtgaaaatt atttattaga cacctttgat cggcgtattc aaattagtgt tatgttgcca
    16321 acttttgaag atcgccccat tcgagaacga ttagagctga ttcaattatt ttttcaaaat
    16381 gaagcggatg ttttacaaaa agatattatt gtttcaagag aagccttgtc tttaatgtta
    16441 tctcatccct ttagtgggaa tattggtaag ctgaagaata tcattaaaat cagttgtgcg
    16501 gatgtgtata gtcgaacaca agaagaacca ttaacgattg ggaaaaatga gatcttgatt
    16561 cagttaaata tgctggaatc cgaagtagaa agcttgccga ttgctagttt attgataaag
    16621 aaaaatcaaa agataaaaac ggatcgaatc aaaccgtttg attatgattc gattttggaa
    16681 aaaattactc agctgataaa tgaaggtaat ttcaatgaaa atttttcttc gataaagaat
    16741 gaagtacagc atttggtgca tcacattgat aaggattctt ttttatcaat gagtagaaaa
    16801 ttgatgattc agatgtttag aaaggtctgg gtatccgttt taggaaaaac gtacggattg
    16861 acgacgagtg gacctgttgc agaaattatt agcagatcct atgtcatttc cccaagctat
    16921 aagacagact taaccgggat aaatcgtaca ttagctgcaa atctacctcg gacgacttac
    16981 ttatgtaatc agttcttgga gcatttacca ccgctctcag aaaaggacga gaactttcta
    17041 agactgctta ttgtcgtgac tttatctgat tatgtagatg aatccatcga gctgaagggc
    17101 ctgttgcttg ctcacggaga tagtacggct agcagcattc aagcagtagt gaatcaatta
    17161 tgcggcaatt atgtttttga agcgttggat atgccagtag agagtaatgt gtcagagatt
    17221 gtcgaatcga caaggaattt cgtgaaacgg caagtgaata cagatagtct agtgctgatc
    17281 gtagatatgg gctctttaaa tcaaatttac acacagatca agaacgattt aaaaggggag
    17341 ttacttcttc tgaataattt gacgacttcc attgcacttg atattggctt gaagatgacg
    17401 atgaatgcgc catttaaaca gattgcggag gatgcagcag acaagtattc aatcaatgtt
    17461 caatactttg aaggcttttc tcaaagtagc aacattatca tttcctgtat gtcgggtttg
    17521 gggatttcgg acaaattgaa agatgttttt cgtcatcatt tatcggatga atccatcgaa
    17581 gttttcacga aggactacag agaactacgt gaattaatcg accaaaatga tgaaacttat
    17641 tttgacaaga caaaattggt catcacgact tcggatcttc cacgttcctt ttcaattcct
    17701 aatattaatg tttatgacat cttagacggt gatggagcag ccaatttgga gcatattttg
    17761 agtcctgaaa tgaagccggc agaatttaac cgcttgattc aggagctagt aaaattcttt
    17821 tcgttggaag gaattgcgga ccgtttgaac tttctgaacc caacgatcgt gatgagtgaa
    17881 gtggaaacag tcatttctaa atatgaaaat tactatcatt tcactttaaa tggccgtgta
    17941 aaactaaatc tctatatgca tacggcattg atgatggaac gtttattctt ggctcggaca
    18001 gaaaaatctg aagaaccgaa ttatgatttg aaaccagatg aagatgaatt ttattatgtt
    18061 tctagaagta tttttcaatc aatggagatg aaatataatt ttaaagtcaa tgcttatgag
    18121 ctttcgctgc tctatgaatt attaggaccg tatattcaaa aataaagtgt ctaaaaaagt
    18181 gtctaatctg ttttttttag gttagacact ttttttaatg tgaattaacg tgattaaacc
    18241 aatgttttta aaagttggca caggaattgc ttatataaaa gtgaacagaa agaaaggaat
    18301 taaccatatg aagaaaattc taatagcgac tcacggtcac ttagcttcag gctttcttag
    18361 cagtattcaa ttactgacgg ggaaggttca agagattacg gtgattaatg cttatgtgga
    18421 tgagtgtgat tttgaacagg agttgaatgt atttgtaaac caggtgaacg agcaggatca
    18481 gatttttgtt tttaccgatt tatttggcgg aagtgtcaat cagaagatta ccaaaacctt
    18541 tttggagaaa gagattgctg taactatttt gtctggcttt aatctgccaa ttctgctcga
    18601 aatagtattg gcaaccaagg aattaacaaa ggaggatatt caggaactgg taaataaatg
    18661 ccaacaggaa ttaaagatga ccattcttga tgaggtacat gtagtaaatg aagaggaaga
    18721 ttttttttag gaggaagaaa aatgattaaa gcagtgcgaa ttgatgaacg attaattcat
    18781 ggacaagtag cgatgacttg gacaaagagt ctaaatttaa ccgggttagt agtagcaagc
    18841 gatgaggcgg ctaccaacga tattcaaaag atgacattga agatggctgc accatcgaat
    18901 gtcaaagtca ttattaaaac agttgatgac gcgattcatt tgttaaaaga tccgcgggcg
    18961 aaggacatgc gcttgatggt tttagtccct aatgtcaaag atgcggtaaa agttgcgaaa
    19021 gctttcccaa cggaaatcga attaatgaac gtggggaatg ctgggaaaat gacaccgcca
    19081 tcggaagata agaaaacctt atcaaaggaa gtgatgttaa cgacgtcaga aattcaagac
    19141 ttaaaagaat tagttgaact ttatccagat accgtttttc agcctacacc ggcgatggaa
    19201 aaacgttcag ctatctctat tttgagtgat ttttagaaaa aggaaggaga aaaattatgt
    19261 atgatgcatt tgtagtcgct ttggccgttt ttattggagt tgctggacat gaaatgttcg
    19321 ggatgtccat gctgagtcgt ccgattgttg ttgctccgtt agcaggagcg ttgatggggg
    19381 atttgcagac ggggttgatt gtcggggcgt cactagaagc catctttatg ggtgtcgtca
    19441 atatcggaat ctctagtacg gcggagcctt ctttggctgc tgggctggct acagcgttcg
    19501 caatcaaaat gggaggaaac gtggatgcga ttattcccat tgtctttcca ctagcagtat
    19561 taggattaca gttaatgaat atgatttttt cttttgtatg tggaccgatg gctgctaaat
    19621 ttttcaaatt tgcaaaatct ggtgatcaaa aaggaatcat caatctgcac ttctttatgt
    19681 ggtttgtcca ctatgcgctt tatgctctta ttccattctt tgccgtttta tttggagcag
    19741 atgttgtcaa aagcgtcttg gataatattc cagaggtcat catgaacgga ttaacagtcg
    19801 ctggaaatct gctgccggca gttgggatgg cgatgctgtt acggatgctt tggagtaaaa
    19861 atattgctgt gtggtttttc tttggggccg ttgttatggc gtatctcgag ttaccattga
    19921 ttgcaattgc cgcagttgga acgattattg caattgttat agcccaaaga gatctacaac
    19981 taaaaaatat atctgttcat gcagttcaaa gcgatgctgc tgtaaatgca acgcttgacc
    20041 aagaagagga ggatttcttt caatgaaatt agctgatgga atttctaaag atgagaaaaa
    20101 attggttcgg aagatgtttt ggcgctcgat gactatgtat gcttcggtga atccggtgac
    20161 gatgggcggc ggtggtttcg cctattctat gatgcccttc attaatcatt tctacaaaag
    20221 tgaagaagat cgagctgcgg cgctggaacg tcatacacag tatttttcga cgaccattcc
    20281 ttgcgcgtcc tttgtcatgg ggattgcggc atcgatggag aaggaaaatt ctgaaaaagc
    20341 tggttttgac gtggaaagta tcaacgcgat caaaacgagt ttgatgggac cattggctgg
    20401 aattggcgat tcattgttct ggggagtttg gcgtgtcgtt accgcgggac tagcgatcaa
    20461 cttagccaat gccggaaata ttttggcacc aattatcttt ttagttatgt tcaatatacc
    20521 taattacttg atgcgatact atggcggcgt tttaggctat tcattaggct ctaaatatat
    20581 tgaaaagctg tatgcgaacg ggttaattga aattctgaca aaagctgcta gtatcttggg
    20641 tctgatcatg gtaggtgcaa tgacgatcag tatggtcaaa ttcaataccg ttttgagctt
    20701 ttcaatgaag ggcgaagaac taatgaatgt ccaagaaatc ctcgatagta tttttaaagg
    20761 aattgtaccg ttattggtaa cattgagctg tttcaaatta ctagataaaa aagtcggcgt
    20821 catcaaaatt atttttggaa tggtcgtctt gggaatttta ttgtcattac ttggaatcgc
    20881 atagtaggaa gctgcagcta ctaggctgca gtttttttag caaggaggga aaagatgact
    20941 attaatgaag taatttattc acccaaacac ccatttttta atgctgagtt aaacgacgga
    21001 aatgtgacta cttcaaacga atacagctac tatcggaata ttcctgatgt aaagggattg
    21061 accttcgatg taagtaatca aaagacatta gcaaaccttg atgtttgcgg gacgattcaa
    21121 acgttgacct tttatcgaga aaatttacta actgaggaaa aaccaggtgt ttgggtcaat
    21181 aaacagctct ctcaaaccaa ttcgttatcc ttaaaggttg aagtaaacgg gattgtctac
    21241 catttgtcag aatcggatca tcgtattgaa gtcgatgtga ttcaagactg tttgcctaga
    21301 tatattcatc attattcgac gtttaaggtc attgtagtat cctttgctcc catactggct
    21361 aataagcgtt taagtatgct tgttcaacag gtttacattg tgaatgaaac acacgaaagc
    21421 cttcaagttt cagtgaaaga tgtgccttcg tatcaagaga agtattcgga tcagcaaaat
    21481 gtgttgattt ctcaaaaagg aaataaggac acgatcgcac aacaggaagt ggcaagcttc
    21541 gccattgctt ttgtggaccc aaatgctttt gaagaagtaa tgacttttca ggaatctgat
    21601 atcgaaaaat ggctgaagga aacattgaac tattttgctc agctatttgg gcagctgtca
    21661 atgccaaacg atagagttgt ccatctctat cgtcgtgcac tttatcaaag cttttctagt
    21721 ttcggtatga atcgacaggg acaggtcgtc ggcagcaatt ggggcagcta tcctgctact
    21781 aatcgaattt ggaataagga tatgtattac gcatcattgc cattcaccat gtttgaaccg
    21841 gagttatgtc aaaaaacggt tctatggttt gatcaatatg gcgttaagtt tcctggttca
    21901 aaatttacag gaggtatcaa tcattctctg agtaattcgt tatccagtgc gttactggct
    21961 agtctttatt ttgaacatac tggggataca aaattctttg agcggtgtcc gcaggttcta
    22021 ctgaatgctt caagaattat tgaggacata ttagctcaac gtcggtcagg ggaaccaatg
    22081 ctttttccat ctgtttggtt atcagacgcc tttgcacttg gaaagtatca taccggcagc
    22141 aatctttgtc tttggaaggc ctgtattgga cttgcagaaa tttttgaagc cctagaacaa
    22201 ctgagtctta gcaagcgata caaaaacatt gcctgtaaac taaaggaatc aatttgtaaa
    22261 caaatgacta tcgatggtcc ttttggtgag caatttttgg agggaatcgg tgatgaggag
    22321 aaaacaacct attcagtgaa gcactatcaa aaaccgattc ttgagcaagg gttaatcttt
    22381 ttaagtgatg tgattgtgga tggaaaaatt aatttattga tgcatgatgg agaagaatca
    22441 gacactacgt tgatgccttt ttatcaattt ttagataata aagatcattt atatcagaac
    22501 accatggcct tttctgctag ttctttcaat ccaacctata gcgaggaaat caaaggcatc
    22561 acttggggat tagaatccgg cgccactttt cctggcttta tcactgtttt aatgagcgag
    22621 ctgcacaacc ctcaagcttt cgtaacccga ttagaagaac taatttgttt ggcagattta
    22681 gacggttcgt ggtggtggtg gccgtatcgc ttagaggctc aacaaggaga agtcgtgcga
    22741 gactttggct gcggcaaatg cggctgggca tcgggtcttt ttgtcagcct aatgatcagt
    22801 caatatttcg gattgaaatt caaaaaagga gtattgcaaa ttgatcctgt tgataattta
    22861 acgttttctt ggaagaattt gaagttcggt caagccttca tttcgattga atgtacgcaa
    22921 tccgagttga gtatttcaaa tttgtcagaa aagccattga aaataagtga tacgaaaaaa
    22981 attcttcacg tagaaaaggg aaaaacgatt cacattcaaa ggagaaaaag atgagtatac
    23041 taaaaaatat tacccaattt aaaaaaatcc agagtcgagc aatcactccc gaaaatccaa
    23101 caggcgagaa aggtaaagct gcgatgttgc caagcgagct aggaccaagc cgcaaaggtc
    23161 gtggcgcaat tccgctgact tctggtgaag aaactgtgat tgcggatatt actggaacgg
    23221 gaataattcg tcatatgtgg atgacgattc gtgatcgaac agaatttggc agttttgttt
    23281 tacgtgattt aatcttaaga atttattggg atgggtcgga tagccctgct gttgaaagtc
    23341 ctttagggga tttcttttgt aatgggtttg gagagcgttg cgaggtgaac tcgttgccga
    23401 tagtggttaa tcctacaggg ggctttaatt cctatttcga aatgcctttc caaacatccg
    23461 ctaaaattac gatcgagaat ttacatccga aggatatgtc ctcatttttt tatacgataa
    23521 attacgcttt ggtggacgaa ttggaagaaa atagcctata ttttcacgca aattggcatc
    23581 gggaacgcat taccaaatta caggaggatt ataagttaat tgatcgatta aagggtcaag
    23641 gctattacgt gggtacttac cttgcgctta cctgcctaga acgttattgg tggggcgaag
    23701 gcgagtttaa attctacatt gatggggata gtgagtatcc gaccgtaacc tcaacaggtt
    23761 cagaagatta ttttggcggg gcgtgggcct ttcatgagta cgatgagttc ggacgtccac
    23821 atgcgaaaaa ctattcaaca gcctttttag ggtatcctta ccaatcaaat agagatgcca
    23881 caagagatcg ttttgaaacg ggaaaattga atgccgttca tgcctttggt gacgacgcaa
    23941 tgccacgaca tggtctttat cgttggcatc ttcaagaccc catctgtttt accgaagatt
    24001 tatcggtcac tttacaacaa atcggcaacg atgatgttcg cttatttgag cggcaagacg
    24061 atgttgctag tgtcgcttac tggtattcaa cagaagcttc aggaaatact aaacgtcatt
    24121 tcactgatcg agaacgcttg ccgcgttaat gaagtgtttt tcacatattc gaagggagga
    24181 acctatgatg atatatgggg caattgaagc tggagggact aaatttgtct gtgcagtcgg
    24241 tactgaggaa ttaacgattt tggaaagagt cagttttccc acaacaactc cagttgaaac
    24301 aatgaagcag gtcattgatt tttttaaacc ttttaaggaa caattggcag gcatcggggt
    24361 cggctgcttt ggtccaattg atattcaatg caaatcaacg agtttcggtc atatcacatc
    24421 aacaccaaaa cttgcttggc aaaatttcaa ttttgtagga acgctaaagc aatatttcaa
    24481 tattccgatt gaatggacaa cagatgtcaa tgcggcttgc tatggtgaat atgttgctgg
    24541 ttctggcaaa gggttagcag gtgttgcata cttcactgtg ggaacaggta tcggaggtgg
    24601 tgccttgttc aatgggactt tcgtggaagg gttcagtcat ccggaaatgg ggcatatgtt
    24661 gatcaagcga catccaaagg acaatttttc aggaaactgt cccttccacc aagattgttt
    24721 agaggggatg gcagcaggac cagcgattga aaaacggttg ggtgtaaaag gccagaattt
    24781 attggctgac gattcgtttt ggcagattga agccttttac cttgcccaat gtgcatacaa
    24841 tacaacactg atgttttcgc cagatagaat tatttttggc ggtggtgtaa tgaaacaaga
    24901 acacatgaag aaaaaagttc aggataaatt tgttgagctt ataaatggat atgttgaaat
    24961 accaccaatt gatagctaca ttattacgcc cgaattaggt gataatgccg gaataatcgg
    25021 cggattagca ttggccagaa aagcggtaag aaataagcaa ccataattaa tgaatgtttc
    25081 attgatactc atgtcaaaag tatttaaccg agtaatcaaa tgatcaaatg aagttggata
    25141 aagcgaaaat gaattcaaat aaaaaggctc taagtcaaat cgtgttgttt tcattaaatc
    25201 actgaaagga aatgcttctg cgtttccttt tttctttttg ttgtgcgctg tataccttga
    25261 ggatattcac tggtggcgta cacttaaata gtaatatatt gtgttacttt atgtctgtgg
    25321 taatagttat agttgcttac caatgtatgt tcatttttcg aaatgttgac ttcataatcc
    25381 ttggcttaat gtttgaattt ttttggtgat agattttttc agccgtataa aataaatcgt
    25441 aatt
//
LOCUS       DAXLPI010000029        25235 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXLPI010000029 DAXLPI010000000
VERSION     DAXLPI010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.029"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            51..326
                     /locus_tag="ACF3T5_001050"
     CDS             51..326
                     /locus_tag="ACF3T5_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFD3631674.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            332..1099
                     /locus_tag="ACF3T5_001051"
     CDS             332..1099
                     /locus_tag="ACF3T5_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFD3631675.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1267..3096
                     /gene="typA"
                     /locus_tag="ACF3T5_001052"
     CDS             1267..3096
                     /gene="typA"
                     /locus_tag="ACF3T5_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFD3631676.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3357..5129
                     /gene="recQ"
                     /locus_tag="ACF3T5_001053"
     CDS             3357..5129
                     /gene="recQ"
                     /locus_tag="ACF3T5_001053"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFD3631677.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5243..5545
                     /locus_tag="ACF3T5_001054"
     CDS             5243..5545
                     /locus_tag="ACF3T5_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFD3631678.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5548..6711
                     /locus_tag="ACF3T5_001055"
     CDS             5548..6711
                     /locus_tag="ACF3T5_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFD3631679.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6737..10165
                     /locus_tag="ACF3T5_001056"
     CDS             6737..10165
                     /locus_tag="ACF3T5_001056"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFD3631680.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10186..11310
                     /locus_tag="ACF3T5_001057"
     CDS             10186..11310
                     /locus_tag="ACF3T5_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFD3631681.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11291..11737
                     /locus_tag="ACF3T5_001058"
     CDS             11291..11737
                     /locus_tag="ACF3T5_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFD3631682.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11721..12059
                     /locus_tag="ACF3T5_001059"
     CDS             11721..12059
                     /locus_tag="ACF3T5_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFD3631683.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12178..12738
                     /gene="rsmD"
                     /locus_tag="ACF3T5_001060"
     CDS             12178..12738
                     /gene="rsmD"
                     /locus_tag="ACF3T5_001060"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFD3631684.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12735..13226
                     /gene="coaD"
                     /locus_tag="ACF3T5_001061"
     CDS             12735..13226
                     /gene="coaD"
                     /locus_tag="ACF3T5_001061"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFD3631685.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13216..14259
                     /locus_tag="ACF3T5_001062"
     CDS             13216..14259
                     /locus_tag="ACF3T5_001062"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFD3631686.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14418..15146
                     /locus_tag="ACF3T5_001063"
     CDS             14418..15146
                     /locus_tag="ACF3T5_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFD3631687.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15262..15507
                     /locus_tag="ACF3T5_001064"
     CDS             15262..15507
                     /locus_tag="ACF3T5_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFD3631688.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15504..15722
                     /locus_tag="ACF3T5_001065"
     CDS             15504..15722
                     /locus_tag="ACF3T5_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFD3631689.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15858..16397
                     /locus_tag="ACF3T5_001066"
     CDS             15858..16397
                     /locus_tag="ACF3T5_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFD3631690.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16390..18663
                     /locus_tag="ACF3T5_001067"
     CDS             16390..18663
                     /locus_tag="ACF3T5_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFD3631691.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18701..19747
                     /gene="holA"
                     /locus_tag="ACF3T5_001068"
     CDS             18701..19747
                     /gene="holA"
                     /locus_tag="ACF3T5_001068"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFD3631692.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19836..20090)
                     /gene="rpsT"
                     /locus_tag="ACF3T5_001069"
     CDS             complement(19836..20090)
                     /gene="rpsT"
                     /locus_tag="ACF3T5_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFD3631693.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20490..20759)
                     /locus_tag="ACF3T5_001070"
     CDS             complement(20490..20759)
                     /locus_tag="ACF3T5_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631694.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            21126..21311
                     /locus_tag="ACF3T5_001071"
     CDS             21126..21311
                     /locus_tag="ACF3T5_001071"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631695.1"
                     /translation="MSTLQEKQLQFNPHLLRNEEIFRGASIYKIITIDMMKQIKARDK
                     QIELAENIVDYLLHTNF"
     gene            complement(21395..24361)
                     /locus_tag="ACF3T5_001072"
     CDS             complement(21395..24361)
                     /locus_tag="ACF3T5_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFD3631696.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SEKVVSLNSLRPLKLS"
     gene            24507..25082
                     /locus_tag="ACF3T5_001073"
     CDS             24507..25082
                     /locus_tag="ACF3T5_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3631697.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
BASE COUNT         8325 a         4331 c         5195 g         7384 t
ORIGIN      
        1 accacttcct atgctacact gaagaggaat atgaaatagg agagttgaag atgcaagaat
       61 atcagtatcc acttgattta gattggacaa cagacgaaat ggtcattgta atgaagatgt
      121 gggaagatct ggaacgtgcg aatgaaaaag ggataaacaa tgaagctttt ttgaagactt
      181 atcagcagtt taaaactgtt gtcaaatcca ttggtgagga aaaacgattg ggacgggaat
      241 ttgaaaaagc ttccggttat tctttatatc gtacagtgca gcaagcaaaa aaaaatccaa
      301 acaaaacttt gagaatgaac gggtgagata aatggaacag atgattgatc atatcaagtc
      361 ctggcttgaa gaagctggtg agaaaatcaa atcttctcaa caaacattaa caatctcgca
      421 aaaatcaaat cgaacggatc tagtaacaaa tgtcgatcga aacatccaga attttctaat
      481 cgaaaaaatc cattcctttg atccagaggc gaaaatattg gcagaagaag aagggtgtaa
      541 tcaattatcg gatctcgatg gaagagtctt tatcatcgat cctattgatg gcaccttgaa
      601 ttttgtgatt gaaggggaaa atttttgtat tatgctcgct gtttatgaat ccggcgttgg
      661 aaagcttggt ttcatttatg acgtgatgag aaatgaactt ttttggggtg gaaaaggaat
      721 aggcgtgtat aaaaatgatg agccattgcc tagaccagca aataaatcct tatcggaagg
      781 cttacttggt gtgaatagtt atcttttcgg tcataataaa tatggaatcc gagaaattgc
      841 ggaaagcagc atggggacac gtatgtacgg atgcgctggt cttgaactag tggcaatctt
      901 gaaaggaaac cacattgctt atatttcaaa ccttagccct tgggattatg cagcgggaaa
      961 tgtcttattg gaagagtttg gtatggtaac aagcggtctt tttggtactc ctttgacatt
     1021 tgaaaaaagg gaatactatt tagcagggac ttcttgtgca tatgcagaaa tcgcagacct
     1081 tttgaaaaaa gaaggctgaa aaaaaggtag aaatcattca caaaaatgat aaaaacgagc
     1141 aaaagattaa ttttctcgaa aaaatgaaaa cattttacta tatttcatga aaaaagtctg
     1201 ctataattag cagtcgagta aaatttgccc tgtcataaag aaccaataaa aaaggagtta
     1261 gttacattga attatagaaa cgatatccgt aacgtcgcaa ttattgccca cgttgaccac
     1321 ggaaaaacaa cattagttga tgaactatta aaacaatcag atacattaga cgcacatacg
     1381 caattgcaag aacgtgcgat ggactcaaat gcgctagaaa aagaacgtgg tatcacgatt
     1441 ttagccaaaa acacagctgt agactataaa ggtattcgcg taaacatcat ggatactcca
     1501 ggacacgcgg acttcggtgg cgaagtagaa cggatcatga aaatggttga cggcgttgtg
     1561 ttggtcgtag atgcgtatga aggaacaatg cctcaaacac gttttgtatt gaaaaaagca
     1621 ctggaacaac atatcacacc aatcgttgtc gtaaacaaga ttgacaaacc ttctgctcgt
     1681 cctgagcatg tcgtagatga agttcttgaa ctattcatcg aattaggagc agatgatgat
     1741 cagttagact ttcctgtgat ttatgcatct gcactaaatg gtacgtcaag cttgtcagat
     1801 gatccagcag atcaagaacc aaccatggca cctatttttg atacaattat tgaaaaaatc
     1861 cctgctcctg tcgataacag cgacgaacca ttacaattcc aagtttcatt acttgactac
     1921 aacgattatg taggtcgtat cggaatcgga cgtgtattta gaggaacaat caaagtagga
     1981 gaccaagtag cattaattaa attagacgga acagttaaga aattccgtgt aacgaaatta
     2041 tttggtttct ttggactaaa acgtctagag attcaagaag caaaagctgg agacttgatt
     2101 gctgtctcag gtatggaaga tatctttgtc ggtgaaacag ttactcctgt ggatcatcaa
     2161 gatgcgttac ctatccttca cattgacgaa ccaacattgc aaatgacttt cttagttaat
     2221 aattcaccat ttgccggtcg agaaggtaaa ttcgtaaccg ctcgtaagat tgaagaacgt
     2281 ttgatggcag aacttcaaac agatgtatcc ttacgagttg aaccaacaaa ctctccagat
     2341 gcatggacag tttctggtcg tggagaattg catttgtcta tcttgattga aaacatgcgc
     2401 cgtgagggat acgaattgca agtatctcgt ccagaagtta ttgaaaaaga aatcgacggt
     2461 gtaaaatgcg aaccgtttga acgcgtgcaa atcgatacac cagaagaata tatgggtagt
     2521 gttatcgagt ccttgagctt acgtaaaggt gaaatgcaag atatggtcca tacaggaaac
     2581 ggacaaatcc gtttgacatt cttaacacct gcccgcggtt tgattggtta ttcaactgaa
     2641 ttcctttcaa tgacacgtgg ttacggaatc atgaaccata cttttgacca atatctgcca
     2701 atgcttcctg gtcaaattgg cggacgtcac caaggggcgc ttgtttcgat cgatacagga
     2761 aaagcaacga cttattctat catgagtatc gaagaacgcg gtacagtctt cgtagaacca
     2821 ggtacagaag tatatgaagg aatgattatc ggggaaaaca gccgtgataa tgatttgact
     2881 gtcaacatca cgaaagcaaa acaaatgaca aacgtacggt ctgctactaa agaccaaaca
     2941 tctgtcatca aaaaaccaaa acaattgacg ttggaagaat ctttggaatt cttgaatgat
     3001 gatgaatatt gcgaagtaac accagaaagc attcgcttga gaaaacaaat tttagagaaa
     3061 aatgctcgtg aaaaagcatc taaaaagaaa aaataatctg taaaaggatg aagagagaaa
     3121 ccagcttgtt ataaaaaata gctggtttct ttttttgtcc aactcttttc tgtggagaag
     3181 tcatctttgt caatggacat aaattcagaa aatacgccga aagacgttta aaagactttt
     3241 aaaaaactta tttctagaga gtttttgttt cataataaga taatgtttca cgaaaggaga
     3301 tcaacttgtc cttcgaagaa aatcgaagta aaatggcaag agaaaggagc gggaagatga
     3361 acgacttatt aaaacaatac tttggctatg atgagtttag acctggtcaa aaagaaatta
     3421 tccagaaagt cattgatcag gaaaatgtat taggaatcat gcctaccgga agtgggaaat
     3481 caatctgtta ccagcttcca gctttactat tagatgggct tactgtggtt gtgtcccctt
     3541 taatctcttt gatgaaggat caggtggacg cggcgaatca attaggtata cccgccactt
     3601 ttatcaatag ctcgttagat ggttacgaaa cagctagacg cttccaagaa atcgatagac
     3661 agcagtaccg tttactttat atcgctcctg aacgttttat catgccggat tttatccaag
     3721 caatgaagcg atggaacgtc cgtatgatcg ctattgacga ggcacattgt atctctcagt
     3781 gggggcatga tttccgccca agttatttac agatggcgaa ccagctagat caactgccaa
     3841 atcgtcctgt aattgttgct ttgacagcta cagcgactgt gcaagtagct gctgacataa
     3901 aaagactttt aaaaattcca gaaaacaacc atattcagac tggttttgaa cgagaaaatc
     3961 tacgatttca agtaattaaa gatcaaaaaa aagaacaata tctaatcgaa tatttaaaga
     4021 tcaacaaaaa tcaatctggt atcatttatg cagcaacgag aaaagaagta gaccgtctct
     4081 atcatttgct taaaaaattc gatttttccg tgggtagata tcatggtggt ctaaatgaga
     4141 atgagcgaac agaaatgcaa gaagcctttt tatacgatcg cttgcagctg attgtagcga
     4201 caaatgcatt tggtatgggt ataaataaaa gcaatgtccg ttttgttatc cattatcaaa
     4261 ttcctggctc tctagaagca tactatcagg aagctggtcg ggcgggaaga gatggtcttt
     4321 ccagtgaagc catcctgctt ttctctcctc aagatatcca agtccaaaag ttttttatcc
     4381 agcagtccca aagagaagaa gggcaaaagc aaaaagaata tgaaaaatta aaagcgatga
     4441 cagaatacgt gtatattgag tcctgtcttc agcaatacat cctaaactat tttggcgaga
     4501 caagcagtcc gtgcaaccgt tgtggtaact gtttagatga cagagaaata gtagaagtaa
     4561 cgacagaagc acagatggtt ctttcttgct taaaacgtat gggtgagaac tatggtaaac
     4621 aaatgctgat gaaagtctta gcaggatcga aagaacaaaa gctgcaagct ttaggttttg
     4681 gacatttatc tacctatgga ctgatgaaaa accaatcgca aaaagaaacg atgcagttaa
     4741 ttgaatattt gatatcgaat ggctatcttc tgaccatcaa tggtgagtat cctgttttga
     4801 aagtaacaga aagagggata caagtattga aaggacaaga atctgtttat cgaaaggaac
     4861 ccaagaaagt ccagcagcta tcggatgaag aaaccgatac actatttgaa gtgctgcggg
     4921 aactgcgaac ggatctagca tcagaagcag gcgttccccc gtatgtcgta ttttcagatt
     4981 ccacattgaa agaaatgagc cgaatacgtc catcaagcag attggagatg cttcagataa
     5041 aaggagttgg ccaaagcaag cttgataaat atggcgaagc ctttttatct cggataaaaa
     5101 atgcggactc taacgttctg gataagtaga aagaacgaat acgtaaaaat gaataaaaac
     5161 aactggttaa atctgttttt tttgctcctt atgaaaatca aatatgttat aattaaggaa
     5221 ttagaatggg aggtaagcac agatggattc tttgtcgaaa aaacttgcga tccaagtatt
     5281 aaatgatgaa gcagaacgga tcaaacgatt aatccgaaat caaaacaaca gtctctgtat
     5341 ctcacaatgc aaagccttcg aagaagtggt agatacacag atgtacggct tttcccggca
     5401 agtcggtttt gctgtccgta taggagtaat cggaaagaac gagggccagc tgatgttaag
     5461 tgagctagaa cgagaattga atcagttata tagtgaggtt taccaagaaa tttatgataa
     5521 aaaagaagtt ggcaaggagg aatagacttg cctaagaaag tgaaaaaaag gcatctatta
     5581 gactatagca tcttgattcc ctatttaatt ttatgtgtat tgggattgat catggtatat
     5641 agttctacgt cgtatctatt attagaaaac ggacaaaatc cgtccgcatc agtcattaac
     5701 cagagtattt tttgggtgct tagcttgatt gctatcgcac tactttataa aatgaaaaca
     5761 gatgttttga aaaaccagcg tttgatcatg gcagccatcg cagttttgac gattttgctg
     5821 ctgattgttg tgttttttgg taaggagatc aatggggcga aaggttggct gcaaattgca
     5881 ggattctcga tccaacctgc cgaataccta aaaatcattt ctatctggta cctgtctctg
     5941 actttatcta aaagacaaaa cagtgtacaa aaagattttc ttgggactgt gaaacgtcca
     6001 cttgcaatgg ttatcggact tactgcatta gtcgctattc ttcccgattt tggtaatgcg
     6061 gcagttatct ttttgattat tttggttctg cttttagcca gcggagtcaa ttatgtgtat
     6121 actttgattg taggagtagg aggcttttgt ctaagtactt ttacgatctg gctgatcaat
     6181 atcacaaacg gaaagatctt acccggacgt ttacagtata tctataatcg atttgctatt
     6241 taccaaaatc ctttctcaga cgaattaaat aaagggcatc aactagtcaa cggctactat
     6301 gccatgttca acggaggact atttggcagg ggattaggta acagtatcca gaaaaaagga
     6361 tttttacaag aagcacaaac cgattttatt tatgcaatcg tagtggaaga actgggagtg
     6421 attatgggga ttttgatatt agcgttgcta ttctttatga ttgtgcgcat tgtccttgta
     6481 ggtatccgtt caaaagatcc gttcaattcg cttttgtgca ttgggatcgg cgcaatgttt
     6541 ttgatccaag tatttgtcaa tcttggaggg attactggcg ttattccttt gacagggatc
     6601 acgttcccat ttttaagtca aggaggctcc agtttgctga tgctttcgat ttgtgtcggc
     6661 tttgtcctca acatcagtgc ggatgaaaaa cgcaagagct taggtttata ataaaaaaag
     6721 atgtaggagt ggttcgatga aaaaagtttt agttgctaac aggggtgaaa tcgctgtacg
     6781 tgtatttcgt gcttgtacag aattaggaat taaaactgtt ggaatctatg cagaagaaga
     6841 tgaatattcc gtacaccgtt tcaaagcgga tgaagcatat ttagtaggac aaggaaaaaa
     6901 accgattgat gcgtatctag atattgaagg gatcatttct atcgcgaaag aatgcggagc
     6961 tgacgctatt catccgggtt acggtctttt gtcggaaaac ttgaattttg cgcaacggtg
     7021 cgaagaagaa ggcatcattt ttgttggacc caaactgcat catttagata tttttggaga
     7081 taaaatcaaa gcaaaagctg cagctatcga agcggggatt gcttcgattc caggcacaga
     7141 tggaccaatc gcttctatag acgatgcctt ggagtttgcg aaacaatacg gttatccgat
     7201 tatgatcaag gcagctctag gcggcggcgg acgcggcatg cgtgtagctc acgatgaaaa
     7261 gagtgcaaga gagggctacg aacgtgcgaa aagcgaagca aaagcagcct ttggtagtga
     7321 tgaagtgtat gttgaaaaat acatcgctaa tcctaagcat atcgaagtac agattctcgg
     7381 ggatacgcat ggaaacgtca tccatctttt tgaaagagat tgttctgttc agcgccgtca
     7441 ccaaaaagta gtagaagtag cgccttgtgt ctctatgaat gaacagcaac gtcaaaaaat
     7501 ctgccaggca gctgtccaat tgatgaaata tgtcgggtat gtcaacgcag gtacagtaga
     7561 atttcttgtt gaaggagatg atttttactt tatcgaagtc aaccctcgcg tccaagtgga
     7621 acatacgatc acagaaatga ttacagatat tgatatcgta acaacgcaac tgttgattgc
     7681 tcaaggtttg gatctgcata aagaaatcgg attgcctcag caagaaggaa ttaaattaaa
     7741 tggttcagcc attcaatgcc gaatcactac agaagacccg ttgaataact ttttaccaga
     7801 cacagggaaa atcgatacgt atcgttcacc tggtggtttt ggcgtacgtc tggatgtcgg
     7861 aaatgcttac gcgggctatg tagtgacacc gtattttgat tcattgctag taaaagtatg
     7921 tacgcatggt gctacctttg aaacagccat tcagaaaatg gaacgttgct tgaaagaatt
     7981 tagaatccgt ggagtgaaaa caaacattcc atttatgctc aatgtcatca cacatcctga
     8041 attccaatca ggaaatgcaa aaacaacctt cattgatagt acagcaacat tatttgaatt
     8101 tcctcgacta cgagaccgcg gaaacaaaac gatgaaatat atcggtgaaa ttactgtgaa
     8161 tggttttcca ggtatcgaaa gtggcgaaaa accattttat gaagaaccac gtatgcctaa
     8221 agatctaatc acaagaactg attacgtaac ggctaagaat gtattagatg cgaagggtgc
     8281 cgatgctttg gtcgaatgga tcaaaggaca ggaaaatttg ttgttgacgg atacgacatt
     8341 tagagacgcc catcaaagtc tgctagcaac tcgtgttcgg acaaaggatt tcaagcagat
     8401 tgcacgtttg actggagaag gtcttcctga actcttttca agtgagatgt gggggggagc
     8461 aacatttgat gtggcttacc gctttttgaa tgaagatcct tggcaacggt tgagaaaaat
     8521 tcgttcttta atgcctaata cgttgctgca aatgcttttc agaggttcaa atgccgttgg
     8581 gtattctaat tatcctgaca atgtattggt ggaatttgtc aaagaagcag cagcgcaagg
     8641 aatcgatgta tttcgaatct ttgatagttt gaactggact ccacaaatgg aaaaaagtat
     8701 ccaagctgta cgggatactg gtaaaatcgc agaggcagct atttgctata ctggcgatat
     8761 caacgatcca agtcgggcaa aatacaatgt tcagtattat aaagacatgg caaaggaact
     8821 agaacaactg ggtgcgcata tcattgcaat caaagatatg gctgggctgc ttaaaccgca
     8881 ggcagcttat cgtttgatta gtgaattgaa agaaacgaca gatctgccaa tccatctgca
     8941 tactcatgat accagcggga atggaatcat cacttattct gctgcttcaa aagcaggagt
     9001 agacatcgtt gacgtggcaa tgagtgcaat gagtgggaat actagtcagc ctagcatgag
     9061 cagcttgtat tatgcactgg tcaatggtcc acggctgcca gaaatcacga ttgaaaatgc
     9121 acagaaatta aaccattatt gggaagatgt gcgcatgtat tacaaaccgt ttgaaaacgg
     9181 cttgaatgct cctgaaacag aagtatatat gcatgaaatg ccaggaggac agtattcgaa
     9241 tctacagcaa caggcaaaag ctgtcggatt aggtcatcgc tgggatgaaa tcaaacaaat
     9301 gtatcatact gtcaacttga tgtttggcga tatcgttaaa gtaactccat catctaaagt
     9361 agttggagat atggcactat ttatggtcca aaatgatctt acagaagaag atatttatga
     9421 aaaaggcgaa acactaagtt tccctgaatc tgttgtgact ttcttccaag gagaactagg
     9481 gcaacccgtt ggcggatttc ctaaaaaatt gcaaaagatt attcttaaag gcagacctgc
     9541 attgaatgaa cgtccaggtc ttttggctga atcagtcgac ttcaacgaag taaaaaaaga
     9601 attggcagaa aaaattggct atgaaccaaa acaagacgaa gtattaagct atttgatgta
     9661 tccgcaagtc tttttggact atcaaaaagc atataatcaa tttggcgatg ttacattact
     9721 ggacacacca acatttttcc aaggtatacg tttgggtgaa acaatcaatg tacaaatcga
     9781 acgcggaaaa acgcttatca ttcgtttaga tgaaatcgga gaacccgata tcgaagggaa
     9841 tcgcgtactg ttcttcaatt tgaatggaca aagacgtgaa atcactatca aagataattc
     9901 gatcatcagt acggtccaaa cccgattgaa agctgaaccg acaaaccgag aacaaattgg
     9961 agcaacgatg tctggttctg ttttggatgt attagtgaaa aaaggcgata acgttaaaaa
    10021 aggtgatacg ttgatgatta ctgaagcgat gaaaatggaa acggccatcg aagcacgttt
    10081 tgacggagaa gtcgcacatg tatatgtgtc ttcaggagac actatttcgt ctggtgacct
    10141 tttgattgaa gtgactgaga aataataagc gaaaggtggg gaaacatgaa aagactgggt
    10201 ttgttttggc tgattctgct attagttatt gtggcattct acatggaacc agtcttgttt
    10261 ccgccaaaac ttgaagtgaa ggaaaactct gatcaacaaa tatcaaacca aatgacaacg
    10321 tggaaatacc aggagcttaa agcaactggt ttttctactt atatcggaaa accgatcgat
    10381 cagctggaaa aggattttgg ccaagcctat gatcgattaa ctagcggatt tggttttgaa
    10441 acaagatatt atgaaaataa agctagtcat ctcacatttg aagcgaacat ccaagacagc
    10501 agagtcacaa ctgttaaagt actgcaaaca gggcctgctg atatcgcgcc gttttattta
    10561 gggatgacga tgcaagatct tacgaaaatc actactatct atactaattt cacttttgag
    10621 taccatgata cagaagttgg aatcgaacta atggaagagg atatgaatta tcgtccgcta
    10681 attgcttttg ataacgacac ttttgctatt ttattttttg atcaaacaac aaacggccta
    10741 ttttcagttg tgtatttaga taaagatagt cttttgaaac tattgccata tcaagtgttt
    10801 ggtgaaggcc ttccacatta tcaagcagat aaatcggctg attgggaatc aatcaatcgc
    10861 gttaaagaac gaaaaagtac gactttatta aatatgctta gaagagaaga cgaacttccc
    10921 atatacaatc aaacacttgc ctttcaaaac cacagtcagc tgctactaca tgattttttg
    10981 acaaggcctg aggaaatatt gtcagaggaa cgtatggctg attggcaaaa atcgctaggc
    11041 agtgctcaaa cgacagtgaa attctcactg acgaatgatg aattggagaa gttaatcagt
    11101 aaacaaaaat tagaaaatac tagtgggcta tttattcatc cggcgattga cgctactttt
    11161 tcgtttttat attggtacag cgatccttac aaccatgacc gtttcatgga cccgggaacg
    11221 gattctgtag gcattgcttt ttctaaagaa aatatgttag tcttattgca agaagaaaac
    11281 ggagaaagta gtgatagcag tgatcgttaa cgaagagctg tttcaactgg aggatcaatg
    11341 cctccaagtc gctgaaatga taaaaaagag taaaacgatg gcttcttatc ttgaacataa
    11401 acagtcaatg gaagaatctg caaaagtttg ccagttgaaa gaagcgttct tgtctaagaa
    11461 agaagcattt gaaaatgtag cggcatatgg tgagtatgca cctgattatc gtgaaaaaaa
    11521 acgagctgtt cgaaaagcaa aaagacagct tgatatggat gagcatgttg cagctttccg
    11581 atatagtgaa acacagctgc aaagtctgtt ggatcaaatt gtccaagtac ttgccgaaga
    11641 agtttctaat gaaatcaaag tagatgcagg tagcccgttt tttgaaacga aaaaacactt
    11701 tggatgcgga ggtaattgtc atgcaattag acaatagtga atttgaaata acaaaacgac
    11761 gcgggctgat cgtatgggtc tacagcttaa aacaattaaa gaatctgaaa aagttcggct
    11821 tgttgcatta tgtctcaaga agaatgaaat atgtcgtgct ttacttaaat gaagagacat
    11881 tggaagaaac agaagaacga atccgtaaat tacattttgt tcgaaacgta gaacgttctt
    11941 accgaccgga tatcgaaatg aattttgccg aaaaaatagg aaacaaaaca gtattaaaag
    12001 acgaaggttt cgaaattgaa gagctaaata cagaaatccg attagctgaa catctttaaa
    12061 aatctcttat gagcagtttt taaataaaaa ataacttgga taacgatcga tttttgatca
    12121 ttgtccaagt atttttttgg tatactagta ccagtaccat ttctaaggag tgagtagatg
    12181 cggataatat caggaaatta tggcgggcgc cgtttaaaaa gtttagcagg ggcgaacacc
    12241 agaccaacta cagataaagt aaaagaatcc atattcaata tgattggacc atattttgat
    12301 ggagagacag tattggatct gtttgcagga agcggtggtt tagctatcga agcagtatct
    12361 agaggctgca gtcatgcggt atgcgtagac aaaaattacc aagcgctaaa aatcatcaaa
    12421 gaaaacatcg aaattacgaa agaaccagag aaatttacca caataaaact tgatgcggac
    12481 aaagcaatcg aacggctagc agcggagaaa caacaattcg actacttgtt tcttgatccg
    12541 ccatatgcaa aacaaaaaat aatcgatcaa atccataaaa tggaagaact gggacttttt
    12601 tctaaaaccg cagttatcgt atgcgaaacg gataaaacag tcgaattgcc agaaactgtg
    12661 gctggattcc atcaaataag aaaacagatt tatggcataa cggccgtaac tatctataga
    12721 aaagagggaa atcaatgaaa cgtgcattat tcccaggaag ttttgatcct tttacaaaag
    12781 gtcacttaga tacggtggaa cgtgcggcta aattattcga tgaggtagta attggtgttt
    12841 ttatcaatac ttcaaagaaa agtttgtttc ccccagaaga aagaatgaca ttgatcacaa
    12901 aagctgtttc tcatcttcct aatgtaaaag tcatgcatca agaaaatcaa ttgacggtag
    12961 aaacagcaaa agagattggt gcagatgcgt tgatcagagg catacgaagt atcaaagact
    13021 ttgaatatga acgagagatt gctcagatga atcatcatct atatgatgaa ttggaaactg
    13081 tcttcttatt agcaaaacca gaatatagtc acgtgagttc cagtatttta aaagaagtac
    13141 tgcactttgg cggagatgtg agcagctact taccgccagt gatcaacgaa gctttagctg
    13201 gaaagcgtga atcaaatgaa agctaaacaa tggttgaaaa cagtactgat tatcatattt
    13261 ggaatgttga tcattggatt tgtagtagtt cccttgccgt attatgtaga atcaccggga
    13321 gccacgatcg atctgaaaga actaattacg gtcaatgata aggaagataa aggttctggg
    13381 tctttctctc ttacctctgt tggtatcagg caagctacag gtttcttagc tttaaaagct
    13441 aaaatgaccc cttttgaaga attgatcagt gaagaagaat tgactggtgg ggcgacaaat
    13501 gaagaatata atcaaatgca acagtattat atggattctt cccaaaatgc agcaatcgaa
    13561 caagcgttaa aacttgcaga caagccttat aaaatggaat tcaaaggcgt atatgtcatg
    13621 agtgtagaga aaacatcgaa tttctttggc aagctttcag ttggagatac agtaacaaaa
    13681 gtagacggga aaagctttca aagtacagaa gcatttatgg attatgtaaa aagccaaaaa
    13741 gttggacaaa caatcgaaat cagttatttg cataatggtg aagaaaaaac agcttctggt
    13801 gatctgatcg agttaccaac agataaaaag gcaggaatag gtatcacgct tactgaccat
    13861 accgagatcg aatcagatac agatgttcgt attaattctg gctctatcgg tgggccatca
    13921 gcgggattga tgttcacttt agaaatctat gaacaagtca ctggtaaaaa cttgcgacat
    13981 ggtaagcaaa ttgcaggaac tggaacgatc aacagtgaag gagaagttgg ccgaatcgga
    14041 ggaatcgata aaaaagtggc aagtgcggat aaagctggcg tagagatttt ctttgcacca
    14101 gatgatacga tttcagacga tgttaagaaa gaatatcctg aaataaaaac caactacgaa
    14161 gaagcaaaag ctgccgctaa aaaattgaat tcctcaatga aaatcgtacc agtaaaaaca
    14221 gtacaagaag cgcttgacta tctagagaaa atgaaatgaa actaaataat taaagagggg
    14281 ttgtgacacg agttctgtca caacccctct ttttcgtcaa aaatcttcac gataaaaata
    14341 aaaacaaaag aaaaaattga cgtaattaga tgttgaaggg acgattttgc cagagctcac
    14401 tgtttcttca attttaaatg aaaggagtaa tgaatgattt ttactggtca ttctttgaaa
    14461 aaagaacaat gatagaaaaa atcagcaaga aaatactagc ttcacccaaa ttgatcattt
    14521 ggatgtctat gatagttctt atcttttgta tcgcaagtgg ttttttatta tcaaatttgc
    14581 tattttatag agaaacagaa gagactgctg caaaagaaat actttcttcg tttagttcca
    14641 gtggagaaga tccatcagct gaaggaaacg atgtagaaga ggatgctcgt ccaaaggtca
    14701 tgtacaccga tatcaaagga agtgtgaagg agcctggtat ctattccttt tcttcagaag
    14761 aacgggtcta tgatgtacta aagcgtgcag gtggattatt agaagaagcg gatagcgatc
    14821 gaatcaactt ttctgcgaag atagaagatc agcaagtcct atatatccct gctgtaggtg
    14881 aagagctgcc agaacatctt gatcagttgg catcctcgga aggaaaacag cctacagcag
    14941 attccgagcc tcccaaaata aatatcaata cagccactcc atcagaactt cagcagattc
    15001 ctgggatcgg atcagtcaaa gcgcaagaaa tcattcgatt tagagaagaa aacggatcct
    15061 tccagaaagt ggaagacctg caggagattt ctggtatcgg tgaaaagaca gttgaaaaac
    15121 taaaaaactt cgttacaatt aaatagaatg attcaaaatc gactggcacg agcaataaat
    15181 ggacgctaat ggatactaag aataatccaa ttctaagagc ttggcaggac gaggaggtag
    15241 tatttttgga taggaaatag cttgattgtt cgcaaagaaa cattaaaaaa accaatgctg
    15301 aatgtctatc tccaaaataa aatatctggg attcatatta tgaatacggc tgtcagcggc
    15361 aataacagtc aagctctacg agaacgcttt gccaaagatg tacttagcta tacagcagat
    15421 aaagtattta ttctcattgg gacgaatgat ctagcagagc ataaacaact ttctaaagaa
    15481 acgtaccaaa aaatctgcag tggatgatag ttcagctgaa aaaagactat agttctgatc
    15541 atgctttctt tatcacgcca ccagcagtcg atgagacaaa acaaaaatat cggaataaca
    15601 agttgatcaa agaatatatc tctgttctca cagaagtagc aaaacgccat gattgtgaag
    15661 tatcgatttt tatacggaat ttcttgaaca aatggattcg aagaattttt ataatgggct
    15721 aatggatgac ggacttcatt tcggcagtga agggtaggat cttttgtcag acaggatgat
    15781 tcgttatatt taaagaatat tttgtttaag caatgagtac tttttaatca acttgaaaaa
    15841 acggaggaat ataaaacatg ttagaaaaat cttcaaaagc atgcgatacg aataaagaaa
    15901 aagttactga taagtcacat aatgaacgca ttccttggga ccagtacttt atggctcagg
    15961 cagtactgtt atctttacgt agtacgtgta cacgtttaga agtaggagcg acactcgttc
    16021 gagaaaaaag gatcattgct ggcggctata atggagcagt cagtggagat gtccactgca
    16081 tagatgaagg gtgttacgtc gtggatggac actgcttgcg aacgatccat gcagagatga
    16141 atgcactcct acagtgtgca aaacttggta tcccgacgga aggttcagaa atctatgtca
    16201 cacattttcc atgtttggcc tgtacaaagg ctttgttgca agctggtgtg aaagggatca
    16261 attatctcca tgactatcga aatgatcctt atgcccaagc gttgatcgaa caattaggtg
    16321 tatctgtaca ccaagttgct ttagactcac agcatttttc aaaacttcaa aatgaattaa
    16381 caggaaaaca tgtctagcgt tttagaaagg catgttcgaa atcggttgat ttttccggca
    16441 attacgtcga tgattatctg tttgttgtat tacactgaca attggggtta tcgtttgctt
    16501 cttgccggaa tttttttatg gttttcgcta aaagaaggac gttttatcgc gtacttgtgt
    16561 attttgtgta gcataacggt atccatttct tataggctac aagagtattc atttttagag
    16621 gaaaaaacaa catctgcgca aattactgta tttacagata cgattcgtgc aaatggtaat
    16681 tttttgacaa tggaaggaag gttatcagag aataaacaaa agctccagct ttcttatcaa
    16741 gtaaacacag aagcagaatt gaaaacatgg cttaacttcc gaggaaaggg aatagaattg
    16801 actgctgaag gcgagttcat ctctccagca aaaaagcgga atctttataa ttttgaccaa
    16861 gaagcttact tcaggaacca tagaatttct ggacggttcc aaattcaaaa gattgaacag
    16921 atcaccatgc aaaatcactg gcgaaactgg cttcagagaa aaagaggatc ttttatctcc
    16981 tttgtccaat ctgaatttcc gcaaaaaaca agtgtatata ttaattcttt attatggggc
    17041 tataaagatg caggattcag agaaccagaa gaaattttta gggagagtgg tttgctgcat
    17101 ctgttcagtc tatcgggtct acacatccaa ttttatttag ggtggctgta ttatttattt
    17161 cgacgtatcg gatggccact acacgtcagt attattccgt tgagtctact aactatctgt
    17221 tatgtttcta ttgcaggaag ttctatttct gttttgcgtg ctagtttgct gtttttattg
    17281 cgattgctag tcaaattgct gaataagcat tattccacca tggacttatt tgcagttgtc
    17341 ttttggcttc ttttactatt tgatcctcga tttctttttc atgcgggtgg acagcttagt
    17401 tttttcatga gttttttgtt tatcctgatt tcttttgatg gagattggtt gaaaaaaacg
    17461 gcctctcacg tcctgtttac tatgatcacg atgccgttga ttgtatggca tttttatgag
    17521 tggcctattt taggtagtct ttttacatta ctgattgcac cggtattcaa gtttcttttt
    17581 cttcctggtg tgttatttct cacactcttc aatcgctggc tgccggaaac tttacttttg
    17641 ctgatagaag agggattaat cttatttgag cgaataatcg atttttcttc agggtcgtct
    17701 ctcattatag gacgtttgcc ccttgtgcta tctgggatgt atattttcgg attactgatt
    17761 tgtatggata ggttgaaaga acaacctgtg aaacacttgt tttatgttgg tcttttttct
    17821 tttatttttt tgactttgcc atttttacgc ttttcttcaa cgatcgcttt tatcgatgtc
    17881 ggtcaaggag acagtatctt tttgcaaagt ccttttcgaa aagaaacgat tttgatcgat
    17941 actggaggtc agttacactt tgaaagagaa aagtgggcgc aaggtatgat acgtcctcct
    18001 gctgaaagaa atatcgttcc atatctaaaa ggtcagggga taagtgtaat cgataaactc
    18061 atcttgacac acgacgatac ggatcatgtg ggagaattac ccacgttatc tcagcatttt
    18121 actatcaaaa aaatttatat cggttggggc gctggacgaa atgagccatt acaaaggcat
    18181 ttatctgaag ctcaaaaaaa tggtactgag atcattgaaa tcaaacatgg ggacaggatc
    18241 agcggttatt atgatcttta tgtattgact ccttttgaaa agggagaggg aagaaatgaa
    18301 gattcgattg gtctatggat ggaatataac aataggcgct ttttattttt aggagatttg
    18361 aatcaagcaa tggaaaaaca attgcttttg atttatccta atctgaaagc cgatgtagtc
    18421 aaattaggtc atcacggtag ccgtacgtca agtaacacag attttctttc ccatattgaa
    18481 gcaaaacatg ggattatatc ctgcggcgta aataatcgtt atggtcaccc gcacagcgaa
    18541 gtattggaga cactagaaga aaatcagata aaaacatggc gaacggatca acaaggaatg
    18601 atcacctatc gctggcatcc agtttttcat cctcatgggc tagtggaaac aatgatagac
    18661 tagtaattct catctgctca tggtaggata atttttgaaa gtggtgagta atttgagtat
    18721 acaagcagag ttacaaaaaa tacgtaaaga aaaacttgca tcttgttact tgattttagg
    18781 aacagaaaag tttctccaag atcaagttcg gacagaaatt gtcaaaaaaa tccaattaga
    18841 gggcgaagat gacttaaatt ttttaagttt tgacatggaa aatgcaagtt tagatgaggt
    18901 agtagctgaa gcagagacgt tgccgttttt cggagatcaa cggttagtat tcgtagagaa
    18961 tccttatttt ttaacaggtg aaaaagtaaa caatggaatc gaacaaaata ctgatttatt
    19021 gacggcttat ttgaaagaac cattagaatc gactgtatta gtattctttg cgccatatga
    19081 aaagttggat gaacggaaaa aagtaacgaa acaattgaaa aaaacagcaa ttatcatcaa
    19141 tgtccaacag ttgaatgaaa aagaagtacg tcaatatctg atgaatacct tagagaattc
    19201 ctcaatcaaa atggaacgaa gtgcaattga tttattcttg aggttgaccg acttagattt
    19261 atccaaaatg atgagagagt tacagaaatt ggtcctttat ggacaaaatc aacagcaaat
    19321 cacggtaaga gaagtagaag aattagttcc taaaacgtta gaacataata tttttgacat
    19381 gacccaatat attttaaaag ggaagacgga acaagctttg cgtttatacg aagatttagt
    19441 tatgcaaggg gaagaaacaa tcaaaattaa tgcgattttg ctttctcaat tacgtttatt
    19501 tttacagaca aaatttcttg taaagattgg ttatcagcag gcaaatattg ctgaaacatt
    19561 gaaaatccat ccatatcgtg taaaacttgc catgcaggaa gtaaaaaaat tcgatgaaca
    19621 tttgctggta cagttgtttg atcaattagt tgagatggat tatcaaatca aaacgggaca
    19681 aatcgaaaaa gaattaagtt ttcaactatt cgttttgcgc acagggcagt tgttaagaaa
    19741 tcgataagaa gaaagacgag ggcaaagcat gcgggaaggt cgtttgtttt tgtccttttt
    19801 ttgacaaata aaaaaatcgg ccgaagccga ttcttttatt tagcgatttt tttgcttaaa
    19861 cgagatttgt cgcggttagc tttgtttttg tggattagac cttttgattc agccatatca
    19921 atagctttaa cagcttcttt atataacgca tctacgttgt cagcacctga agctacagca
    19981 tcttcaaatt ttttgattgc agtacgcata gcgcttgttt gagatgaatt tttcacatta
    20041 gcgttttcgc ttgtacgaac acgtttgatt gcagattcaa tgtttggcat tagtttcacc
    20101 tccgataact taagttctta cgcgtgttac cgcataattc aacattcatt attataccta
    20161 aaggcagcgt gctttgcaat atgaacatga caagtttttt tccttaacag tcttttatta
    20221 ggattttttt tcagtaaaaa cggatagtac aagctgaaaa aaatcaaaaa gacgctattg
    20281 tgggaggcaa tagcgtcaaa ccaaactaga tttttattta tctatttaaa caaagtttga
    20341 atgggaatgt ttggtactaa gttattatat aacaaagaga accgtttgca aagttttttt
    20401 tgaaaaggga tacgattccc tgttgttctt tgtttttcta ttaagaaact gcagttattt
    20461 tataaccagt atcctttgat cagtaaaggc tattctggtg taccgattat tttaatgtat
    20521 tcatcgatat tttttatcaa catttttttt atgaccaact ggttccgacg gttccatttg
    20581 ccacttactt ccagcacata tttatttagg ggcaaacgta agatatattt tgccaaatag
    20641 cgatctttaa gcaggccatt gatcacacgg tcctcaagct gcaagttgat cccagtcagc
    20701 atcattggct gttcttgtaa aacgtacgta tcaattaaaa gaccttcaaa agtttccaca
    20761 agcgtcttcc tttcttttca aagtaacctg aaaacaggca ctaattcttc cattttgata
    20821 ttcgttgaag tggatgcgat cattctttct tttgtccact gtaatctagt ataacaaaaa
    20881 agagcagaag agccaaaaag aaaaaagagc atcgaattct ataggaagtc tcattggcac
    20941 ggcctaaatt attacaacct gaataattca tagtaccttg tcagaacagg catttatccc
    21001 gctttgataa ggtttatcac tctattcctt cttcacccgt tgggtgaaga aaaaaatata
    21061 caaaaagagc acactctatg ttattgtaaa gtcgatcaaa acaacaaaaa caagaggtgt
    21121 gctcaatgtc aactttacaa gaaaagcaac tacaattcaa tccccatttg ttgagaaatg
    21181 aagaaatatt tcgtggagca agtatctata aaatcattac gattgatatg atgaaacaaa
    21241 taaaagctag agacaagcaa attgaattag cagaaaacat tgttgactac ttattacata
    21301 ctaatttcta agaatataaa ggggtagcgt caggaaaatg cggatttaca acgctaagcc
    21361 tattttcctg acgaatccct cgtttttaac aacgttaaga aagttttagt ggtcttaaag
    21421 aatttaatga gactactttc tctgagttaa aatggtattc tcctagtaaa ttaatatgtt
    21481 cccaacctaa gggcgacata tggtgtaaca aatcttcatt aaagctacct gtccgttttt
    21541 tatattcaac tgctgttgtt aggtggagag tattccaaat acttatagca ttgataatta
    21601 tgtttaaagc actggctctt tgcaattgat gctgtatggt gcgttctcta agctcacctt
    21661 gttttccgaa gaaaatagct cttgccaatc cattcatggc ttctccttta ttcaatcctc
    21721 tttgtatttt tcttcttaat gattcatccg atatataatt caaaataaag atcgtttttt
    21781 ctattcggcc catctcacgt aaggctgtag ctaagctgtt ttgtcttgaa taggaaccta
    21841 gcttccccat aataagggat gctgaaactg ttccctccct tatagaatga gctaatcgca
    21901 aaacatcctc ataattttct ttaatgacct ttgtatttat ttgtccacgt aaaatggctt
    21961 ctagttttgg atactcactt gctttatcta tcgtaaataa ttttgagtcc gataaatccc
    22021 ttattcttgg ggcaaattta aatcctaata aatgagtcag tccgaatatt tggtcagtgt
    22081 aaccggcagt gtctgtataa tgttcctcta tgtttagatc cgtctcatga tgtaacaaac
    22141 catccaaaac atgaatcgca tctcttgaat tagtatgaat aatctttgtg tagtaagaag
    22201 agaattgatc acttgtaaat cggtagatgg tggctccttt tccagttcca taatgtggat
    22261 ttgcatctgc atgtagtgat gaaacaccta gctgcattct cataccatct gacgaagatg
    22321 ttgtaccgtc gccccaatag aaaggcaatt gtaatttatg atgaaagttt actaatatgg
    22381 cttgggcttt attcatggca tcttcataca tgcgccattg agatacattg gctagttgct
    22441 tatatgtaag tccgggtgtg gcttcggcca tcttgctcaa gccaatattc attcccattc
    22501 ctaaaagggc agccatgata atgattgttt cttccttatc tggttttcga ttattggaag
    22561 catgagtgaa ttgctcatga aatcctgtta tatgggccac atccatgagt aaatcagtta
    22621 attttattct tggtagcatc tgataaaggc ttgcactaaa tttttttgct tcttctggaa
    22681 catctttttc taagcgtgca agtgatagct ttcctttttc aagagaaacc ccatctaact
    22741 tattggaatt ggcagctaac cactttaacc tttcattaaa gctgctggtt ctctccgtta
    22801 tataatcttc gaatgataaa ctaactgata atctcgtatt ccccttcgat tgattccatg
    22861 tatcttccga aaacaaatat tcctcaaaat ccctatattg tctgctgcca acaatggaaa
    22921 catctcctgc ccgaacatgc tcccgaagtt ctgttaaaac agccatttca tagtaatgac
    22981 gattaattgt tgtaccatca tcctcgtata aatgtctttt ccatcgtttt gaaataaaat
    23041 ccacaggtga gtcatcaggc acttttcgct ttccagattc gttcattcct cggataatct
    23101 caacagcttg taaaagtggc tcatttgcct ttgtagaatg aaattccaat actcttaata
    23161 gcgttggcgt atattttctt agtgaataaa accgtttttg cagtaagtct aaataatcat
    23221 agtcggcagg acgtgcaagt tcctgagcct cttctactga agagacaaag gtattccatt
    23281 caataaccga ttctaaaacc ttaaaaacgt ctaatttttc ctctcttgct ttaattaatg
    23341 cttgtccgat gttcgtaaag tgtataactt tctcatttag ctttttaccg ttttgtttct
    23401 ggatttcctc ttgagcctta cgaccttttg ataacaaact aagtatttgc ctatcatgaa
    23461 tttcaaacgc tttatccgtt agctcctgag taagttgtaa taaatagatg gttaatatcg
    23521 aataacgttt attttcttga aagtcacgga atgcatacgg ctcgtatctt gagcctaagc
    23581 gagacagctg caacaggcgg ttacggtgca aatgactaat ttgcactgtt tctaaatcca
    23641 ttcctcgtat gtattcgagt cgttctatta tttttagaaa agtttcgggt gaaggatgac
    23701 ccggtggctc ttttaaccaa cccaatatcg ttttattgga ttcggatgga tgctgcgagg
    23761 taataatccc ttcaagcttt tctttttgct catttgttag agatttacta accgtattaa
    23821 atagcttctt ttcagccatt gcccttgctt cccacaccat tctttcaagt gtagtgatag
    23881 caggcagtat aattttgttt tttcttagaa aatctatgca ttcatgcagt agatgaatgg
    23941 catcaccatt ttccaaagct aattgatgaa ggtacttaaa tgtcattcga tattcactca
    24001 gggtaaaagt tacaaagtcg tattcacttc gaatttcttt caaatgatcc caaagtgtat
    24061 tttccctttg aggataatga tcaagcgagg atggactaac accaatctgt ttcgatatat
    24121 attgtatgac cgaatctggg atgcttttga tatgagtgta tggccaaccg ggataccgaa
    24181 gaacagctaa ttgaacagca aatcctaaac ggttttcttc cctccttcgc ttattaacta
    24241 tttctaaatc ccgtttggaa aaagtgaagt aggtccccag tatccattca tcttcaggga
    24301 tttgcataaa agcctgtctc tgttccggtg taagcaattc tctacctctc gcaattttca
    24361 ttcagtatca ttccatttct gtattttcaa tttattagtt caattatata tcaatagagt
    24421 gtactctatt gatacaaatg tagtagactg ataaaatcat agttaagagc gtctcataag
    24481 acttgtctca aaaatgaggt gatattttgc ggaaaatcgg ttatattcgt gtcagttcga
    24541 ctaaccagaa tccttcaaga caatttcagc agttgaacga gatcggaatg gatattatat
    24601 atgaagagaa agtttcagga gcaacaaagg atcgcgagca acttcaaaaa gtgttagacg
    24661 atttacagga agatgacatc atttatgtta cagacttaac tcgaatcact cgtagtacac
    24721 aagatctatt tgaattaatc gataacatac gagataaaaa ggcaagttta aaatcactaa
    24781 aagatacatg gcttgattta tcagaagata atccatacag ccaattctta attactgtaa
    24841 tggctggtgt taaccaatta gagcgagatc ttattcggat gagacaacgt gaagggattg
    24901 aattggctaa gaaagaagga aagtttaaag gtcgattaaa gaagtatcat aaaaatcacg
    24961 caggaatgaa ttatgcggta aagctatata aagaaggaaa tatgactgta aatcaaattt
    25021 gtgaaattac taatgtatct agggcttcat tatacaggaa attatcagaa gtgaataatt
    25081 agccattctg tattccgcta atgggcaata tttttaaaga agaaaaggaa actataaaat
    25141 attaacagcc tcctagcgat gccgaaaagc cctttgataa aaaaagaatc atcatcttaa
    25201 gaaattctta gtcatttatt atgtaaatgc ttata
//
LOCUS       DAXLPI010000030        25184 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXLPI010000030 DAXLPI010000000
VERSION     DAXLPI010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.030"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..572
                     /locus_tag="ACF3T5_001074"
     CDS             <1..572
                     /locus_tag="ACF3T5_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFD3631698.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="ACF3T5_001075"
     CDS             576..1217
                     /locus_tag="ACF3T5_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631699.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDFIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="ACF3T5_001076"
     CDS             1214..2131
                     /locus_tag="ACF3T5_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFD3631700.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="ACF3T5_001077"
     CDS             2128..2799
                     /locus_tag="ACF3T5_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631701.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="ACF3T5_001078"
     CDS             complement(3340..3828)
                     /locus_tag="ACF3T5_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFD3631702.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="ACF3T5_001079"
     CDS             complement(4029..4295)
                     /locus_tag="ACF3T5_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFD3631703.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="ACF3T5_001080"
     CDS             complement(4295..4558)
                     /locus_tag="ACF3T5_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFD3631704.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5079..5219
                     /locus_tag="ACF3T5_001081"
                     /pseudo
     CDS             5079..5219
                     /locus_tag="ACF3T5_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..7184)
                     /locus_tag="ACF3T5_001082"
     CDS             complement(5361..7184)
                     /locus_tag="ACF3T5_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD3631705.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="ACF3T5_001083"
     CDS             complement(7782..10487)
                     /locus_tag="ACF3T5_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFD3631706.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="ACF3T5_001084"
     CDS             complement(10793..11740)
                     /locus_tag="ACF3T5_001084"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFD3631707.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="ACF3T5_001085"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="ACF3T5_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFD3631708.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="ACF3T5_001086"
     CDS             complement(12375..12737)
                     /locus_tag="ACF3T5_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFD3631709.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACF3T5_001087"
     CDS             complement(12748..13869)
                     /gene="alr"
                     /locus_tag="ACF3T5_001087"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFD3631710.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACF3T5_001088"
     CDS             complement(13885..14235)
                     /gene="acpS"
                     /locus_tag="ACF3T5_001088"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFD3631711.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="ACF3T5_001089"
     CDS             complement(14365..15876)
                     /locus_tag="ACF3T5_001089"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFD3631712.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACF3T5_001090"
     CDS             complement(16198..17550)
                     /gene="murF"
                     /locus_tag="ACF3T5_001090"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFD3631713.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="ACF3T5_001091"
     CDS             complement(17703..18779)
                     /locus_tag="ACF3T5_001091"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFD3631714.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="ACF3T5_001092"
     CDS             18975..20720
                     /locus_tag="ACF3T5_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFD3631715.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="ACF3T5_001093"
     CDS             complement(21000..21437)
                     /locus_tag="ACF3T5_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFD3631716.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="ACF3T5_001094"
     CDS             complement(21421..22794)
                     /locus_tag="ACF3T5_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631717.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="ACF3T5_001095"
     CDS             complement(23116..23604)
                     /locus_tag="ACF3T5_001095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3631718.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="ACF3T5_001096"
     CDS             complement(23626..24228)
                     /locus_tag="ACF3T5_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFD3631719.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(24297..>25184)
                     /locus_tag="ACF3T5_001097"
     CDS             complement(24297..>25184)
                     /locus_tag="ACF3T5_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFD3631720.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         7213 a         5315 c         4605 g         8051 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgattttatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agcccaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgcc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctt caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaatagcc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa acttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc acggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc ttatgaactg aaccccaaaa gttgaactat
    24301 ttaatggact gtttccgata ttctactgga gatagtccat ttaactttag ttttatcctt
    24361 ttgttattat accacctaat atactcattt aatttacttt gaaatatctc aattgatcgg
    24421 aatttttctc gataaaaaaa ctctgattta agcacaccaa aaaaattttc tattacagaa
    24481 ttatctaagc aatttccttt tctagacatg ctttgaataa tgttattctc ttttaatttt
    24541 ttttgatatt gtggcatctg atactgccat ccttgatctg aatgtagaat cagtgaaccc
    24601 tcagttccct tttttttaat tgcttgttga agcatttctt caatcagttt atatgttgga
    24661 ctagttgata tactataact aattatttct ccgttaaata aatcaagtat aggagataga
    24721 tagatttttc ttccttttat cttgaattca gtgacgtctg tcacccattt tttattgggt
    24781 gtatctactg aaaaatttcg ttttaacaca ttcttggcaa tttttcctac tgttccttta
    24841 taagatttat atcgctttat tcggatttga caggtaagtc ccatttggga cattaatttt
    24901 ctaactgttt tatgattgat tgtatatcct ttcatcttta acgctaaagt gactctacga
    24961 taaccataag agtttcttga ttcttttaca atcgctgtaa tttcttgctt tatcttgcta
    25021 tatttatctg gcttatctaa tttttttacc cagtaataat aagttgactt cgctaattgt
    25081 gcaattgaaa gtaatagatt caatttaaat tcttttttga gctgaaggat tgttttaacc
    25141 ttgatttctt cttgctcaaa tcttgttctt gaatcaaggt ttct
//
LOCUS       DAXLPI010000031        24351 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXLPI010000031 DAXLPI010000000
VERSION     DAXLPI010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.031"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..829)
                     /locus_tag="ACF3T5_001098"
     CDS             complement(<1..829)
                     /locus_tag="ACF3T5_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFD3631721.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
     gene            complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="ACF3T5_001099"
     CDS             complement(1053..2993)
                     /gene="thrS"
                     /locus_tag="ACF3T5_001099"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFD3631722.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3330..3509)
                     /locus_tag="ACF3T5_001100"
     CDS             complement(3330..3509)
                     /locus_tag="ACF3T5_001100"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFD3631723.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3556..5379)
                     /locus_tag="ACF3T5_001101"
     CDS             complement(3556..5379)
                     /locus_tag="ACF3T5_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFD3631724.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5657..7936
                     /gene="helD"
                     /locus_tag="ACF3T5_001102"
     CDS             5657..7936
                     /gene="helD"
                     /locus_tag="ACF3T5_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFD3631725.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8029..8676)
                     /locus_tag="ACF3T5_001103"
     CDS             complement(8029..8676)
                     /locus_tag="ACF3T5_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631726.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8855..9574)
                     /locus_tag="ACF3T5_001104"
     CDS             complement(8855..9574)
                     /locus_tag="ACF3T5_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631727.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9705..10856)
                     /locus_tag="ACF3T5_001105"
     CDS             complement(9705..10856)
                     /locus_tag="ACF3T5_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFD3631728.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11073..11813)
                     /locus_tag="ACF3T5_001106"
     CDS             complement(11073..11813)
                     /locus_tag="ACF3T5_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3631729.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11817..12140)
                     /gene="rsfS"
                     /locus_tag="ACF3T5_001107"
     CDS             complement(11817..12140)
                     /gene="rsfS"
                     /locus_tag="ACF3T5_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFD3631730.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12197..12790)
                     /gene="yqeK"
                     /locus_tag="ACF3T5_001108"
     CDS             complement(12197..12790)
                     /gene="yqeK"
                     /locus_tag="ACF3T5_001108"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFD3631731.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12774..13424)
                     /locus_tag="ACF3T5_001109"
     CDS             complement(12774..13424)
                     /locus_tag="ACF3T5_001109"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFD3631732.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="ACF3T5_001110"
     CDS             complement(13434..13745)
                     /gene="yhbY"
                     /locus_tag="ACF3T5_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFD3631733.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="ACF3T5_001111"
     CDS             complement(13761..14870)
                     /gene="yqeH"
                     /locus_tag="ACF3T5_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFD3631734.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14867..15394)
                     /locus_tag="ACF3T5_001112"
     CDS             complement(14867..15394)
                     /locus_tag="ACF3T5_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFD3631735.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15574..16359)
                     /locus_tag="ACF3T5_001113"
     CDS             complement(15574..16359)
                     /locus_tag="ACF3T5_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFD3631736.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16352..17221)
                     /gene="accD"
                     /locus_tag="ACF3T5_001114"
     CDS             complement(16352..17221)
                     /gene="accD"
                     /locus_tag="ACF3T5_001114"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFD3631737.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17222..18601)
                     /locus_tag="ACF3T5_001115"
     CDS             complement(17222..18601)
                     /locus_tag="ACF3T5_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFD3631738.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18609..19028)
                     /gene="fabZ"
                     /locus_tag="ACF3T5_001116"
     CDS             complement(18609..19028)
                     /gene="fabZ"
                     /locus_tag="ACF3T5_001116"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFD3631739.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19053..19529)
                     /gene="accB"
                     /locus_tag="ACF3T5_001117"
     CDS             complement(19053..19529)
                     /gene="accB"
                     /locus_tag="ACF3T5_001117"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFD3631740.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19532..20767)
                     /gene="fabF"
                     /locus_tag="ACF3T5_001118"
     CDS             complement(19532..20767)
                     /gene="fabF"
                     /locus_tag="ACF3T5_001118"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFD3631741.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20786..21523)
                     /gene="fabG"
                     /locus_tag="ACF3T5_001119"
     CDS             complement(20786..21523)
                     /gene="fabG"
                     /locus_tag="ACF3T5_001119"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFD3631742.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21535..22452)
                     /gene="fabD"
                     /locus_tag="ACF3T5_001120"
     CDS             complement(21535..22452)
                     /gene="fabD"
                     /locus_tag="ACF3T5_001120"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFD3631743.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22559..22783)
                     /locus_tag="ACF3T5_001121"
     CDS             complement(22559..22783)
                     /locus_tag="ACF3T5_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFD3631744.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22836..23798)
                     /locus_tag="ACF3T5_001122"
     CDS             complement(22836..23798)
                     /locus_tag="ACF3T5_001122"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFD3631745.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23808..24248)
                     /locus_tag="ACF3T5_001123"
     CDS             complement(23808..24248)
                     /locus_tag="ACF3T5_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFD3631746.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6657 a         5427 c         4241 g         8026 t
ORIGIN      
        1 attcttggat tgcttctcgt gtttttttca ctttttcgct aagcttttcg tttttagcca
       61 tatagaaata aaaaatgata tcgatcgtag caaattgggc caaaagtgaa ttagttgccg
      121 cacttcgatt cgtgatttct tttggtctag atgtttgtac gagtacatcg gctatcttgc
      181 taagcgggtt atttccaaat tgcgtcaacg caatgatttc gatattcatg cttttcgcca
      241 gctcggctaa tgcgactaca tctgcacttc tacctgagtt tgaaacaagc caaagcactt
      301 tctttttctc attgctgaca agttgcggga gcaagacgtg gatatctttt tcaaaaatga
      361 ttggcttacc gacacgagac catttttgca gaatatcttc tactactaaa gaagaggcgc
      421 caaccccata taaaaaaaca gtctctgccg attccaaaca ctctacgact ttttggatcg
      481 tttcttcttc tagaatatct actgtttctt ttatcgctac ttgtgcattg ttaccaagtt
      541 tgtttttgat agagtaaaag gactcatttg cttctatatc actaaaacct tcatcgattc
      601 cagtctgtag ttctgttgaa atcgtgattt tgaagtccgt cagactttga aaaccgattt
      661 ttttggaaaa acgtacaact gtcggcgcac tcaacccgga agcttctgct aattcattag
      721 ctgtcattgt agggaccttc tcaatattac cgataatgta gttggccaac tttttttctg
      781 ctgaagaata atcttccatc atactttcaa tacgactaat tacgctcata agttcctcca
      841 cccttttaat gatagtctat gtaagtatac catatcagac ccttattcac ggtcgtatac
      901 tggataaaaa acaaaaaaac aatccgccca acagaatatc ttatcccatc aatcccacag
      961 attctattga gcatctgtgt ctttgaggaa tacctgttat ctattgttcg gattgctttc
     1021 tatctactac ttttttacag gaaatttacc tattagtttt ctctgctgta atttttgact
     1081 tctgcttcca tagcatcaat aaagatattc aaatcttcta cagctgtttc tttacttcca
     1141 taacgacgta cgttcactgt tgcatcttcc aattccttgt ctccaacaac taattggtaa
     1201 gggatctttt gtgtttgaga ggcacgtatc ttatagccca ttttttcatt acgatcatca
     1261 acttctacac gcaacccctt catttgcaaa cgttctttga tttcataagc ataatcgcca
     1321 tgtgcatcaa cagaaacagg gatgattgtt gcttgtatag gtgccaacca tgttggaaaa
     1381 gcgcctttgt atacttctgt taaataagcg acaaaacgtt ccattgtaga aacaattccg
     1441 cggtggataa ctactggacg atgcgtattt tccccgtctt ctccaacata tgtcaaatca
     1501 aagcgctctg gcaataagaa gtctaattgt atagttgata atgtttcttc tgtaccaaga
     1561 gctgttttga cttgaacatc tagttttggt ccgtaaaagg ctgcctcgcc ttctgcttca
     1621 aagtaatcta gttctaattc atccatagcg gctttcagca ttgcttgggc tttttcccac
     1681 attgcgtcat catcaaaata tttatctgta ttgtttggat cgcgatagct caagcggaaa
     1741 cgataatctg taatattgaa gtcagcatat actgccacca tcaattcaag cgtacgttta
     1801 aattcatctt tgatttgatc aggacgaaca aatgtatgac catcattcaa cgtcatttct
     1861 cgtacacgtt gtaaacctga caaagcacct gatttttcat agcgatgcat cattcctaat
     1921 tcagcgatac gaattggtaa ttcacgataa gaatggatcg tgtttttgta gaccatcatg
     1981 tgatgagggc agttcattgg gcgaagaaca agcatttcgc catctcccat atccattggc
     2041 gggaacatgt cttcttgata atgatcccag tgtccagatg ttttgtacaa ctcaacatct
     2101 cccattattg gtgtatatac atgttggtaa cctagactaa cttctttgtc tacgatataa
     2161 cgttcgatcg tgcgtcggat tgttgcccct tttggaagcc agaatggcaa accagaacct
     2221 acttctgggg aaaccatgaa caagtcaagt tcttttccta gtttacgatg gtctctttct
     2281 tttgcttctt cacggagacg gatatattct ttcaaagctt tcttgtcaaa aaaggctgtc
     2341 ccgtatacac gttgcatcat ttgattgttt gagtttccac gccaataagc acctgccact
     2401 gataataatt taaatacttg tatacggcca gtggatggaa catgtggtcc acgacacaaa
     2461 tcgatgaatt cgccttgttg ataagcagta atcacttctt cttctggcaa ttcactaatc
     2521 agttctactt tgtacggatc acttgcaaaa atttctaatg cttcctgttt tgaaagaaca
     2581 cggcgttcga tcggcagatt ttctttgacg attttcatca tttctgcttc gattgccggc
     2641 aaatcttctg cagttacttg gttttgtcca ttatctgtat cgtagtaaaa ccctgaatcg
     2701 atagctggtc caacgccaaa gtggatattt ggatagaggc gacgcatggc ttgggccatc
     2761 aaatgagctg ctgaatgacg gactaagcca agtgcatctt catggtctgg tgtgatgatc
     2821 tcaatacttg catcttcctc gattggacga gtcaaatcaa cgagttttcc gttcacttta
     2881 ccagctaaag ctttttttgc taagcttttt gaaatgcttt cagcaattgc caatgttgtt
     2941 gttccagact caaattcttt gactgcacca tctggaaaag taatttttat cataatagtt
     3001 tcctccttga aatgttttga ttgatcagat catcaagctg aaaaacaaaa aaatcctagc
     3061 atctcttgaa aagagatact aggacgaaaa gttcgtggtt ccacctaatg ttcaaacgca
     3121 aatgcgttcc tcaagcgttg taacggtccg accgcccttg cttcaacaag ggtttcgaaa
     3181 gtggtcatcc tttggttttc tcctggaaaa gtttcagcct agcttttcct ctcttatcga
     3241 agactctcaa attcagttgg ctttctcatc aatcaattat gtgtttattt aaccactaat
     3301 aaatagaaaa atcaagtggg aaactaaact tattttttcc attccccatt tacaccgtat
     3361 gttccttttt tcatctcatt gatgatgaca tggatgtgtt cttttggtgc ctgcgtgttt
     3421 ttatgaacag cttccgtgat atctttcatc atattttcta attgttccgg actgcgacct
     3481 tctactaatt cgacatgtac gaatggcata atgctctctc ctctttctga tcgttctttt
     3541 ttcaactcga ttctatcata ctatgtttgt cttgggtact gaatcaggaa aaatggctgg
     3601 atcttttttt gctctcaagg cactcaaaat agagaccgta tccacgactt cttgcaaaac
     3661 agctccgaat aatgcgggga ttagtcccgt acttgcaatt aacatcaaaa aaatacaaat
     3721 aaagatgcca atcagtacag actgtttcgc tattctcatc gtatctcttg aaatcactac
     3781 tgcttttgct acacgcaaga gatcatcttt caagatgacc gcatctgcac tttcactagc
     3841 agctgtagaa ccatgagccc ccattgcaat accgatatct gcagcagcaa gtgctggtgc
     3901 gtcattcacg ccgtctccta ccatcgcaac aggtctttga tctttaggga tactttctaa
     3961 aatggtcagt ttatcttgag gcaagctacg ggcatgtaca gaatctaatt ggactttttc
     4021 tgcaatcgtt tgagcaatgg cagatccatc cccggtgatc atcatcaaat ttttcacatc
     4081 aagttttctt agctccttga ttgtctttgc tgcttctggg cgaatctgat ccgaaaatag
     4141 gatcgttcct gcaaaagatc gatcaattgt tacatagatt gctgtattgg acgtttctat
     4201 ttcttcagct gaaacaaaag aggcacgtcc aatctttact tcatgattat tgactactgc
     4261 ttggattcct tgaccggtca cttctttcag ttgactaaca ggatacagtt ggctgttttt
     4321 cacgttgttc actaacgaac gagctaagat atggctagat tcctgttctg cactagctgc
     4381 atacagaagt agctcttctt tttttattcc attgactggc tgaatcgcct ctacttccaa
     4441 tatccctttg gtaagcgttc ctgttttatc aaaagcaatc gtttttgtac gtgcgagctt
     4501 ttcaatcgtt gttccagttt ttacaacgat cccgttttta cttgaacggc tcattcctgc
     4561 aactaaagca atgggcgcag ctagaatcag tggacaaggt gatgccacta ccagtacctc
     4621 agcaaatcgt aaagggtctt ttgagaacca ccaagaaata ccaccgatca aataagcaat
     4681 caacgtgaaa gggactgcat aacgatcagc taaacggaca aatcgggcag gcttttcttt
     4741 tgattcttcg actagtttaa taatcatttg atactggctg tctgcggcac gtttttcagc
     4801 gatcatttgg atcgatccgt ctccatttaa agaccctgac ataatctgat ctccaggttt
     4861 cttggtttcc gggcgggact cacctgtcaa agaagattca tcgaccgaag attctccttt
     4921 ggtcactcga ccatctgcgg gtacaagttc gccaggcttg actagtaact ggtcatcaat
     4981 atttatttca tcgattacta tatcctgcaa ttttccttct ttgaatcgat gggcaattct
     5041 tggggtgtta tctaacaaag actgcagttc tttacccgct cgtttattcg cgtaatcctc
     5101 tagactatct ccgcccgtaa gcatgatcaa tatcatcaaa ctagcccagt attctttgac
     5161 ggctaatgtt gcaaggattg cggtaatcgc taatacatct acgccatatc ttcctgaacg
     5221 taacgtcttg atcatttcca aaaacatcaa gaaggcgaga atccctccag taaatgcgac
     5281 aatcgcaaat gcaagggccg gctggtgaaa aacaaattca cttaataaag ctatcccacc
     5341 aataagcaaa gtaagtaaaa attttttgaa atgtgtcatt gttttttctc ctcttttatg
     5401 tgacttagag aggttagtaa taaatttcca gctgcctctt tcatcttaag catagtcctt
     5461 gactagcact ttttaaaaca ataggcctta attgaaaaca actatcacta tcagcgtttc
     5521 caaaattcat cagttctaca aacaaaaata ttcagcaagg attacttttt cgattcatca
     5581 gttaaaaagt accttttttc gaaaaactat gctatacttt taattcgtta tttacggcgg
     5641 aaggagcaaa catgcgatga atgagaaaca actagaacaa acgcacgtgg atgaaacgat
     5701 cgaactaatc cagatcgaac aaaatatgct ggatacaaaa caacaagaat tgactcagga
     5761 aatgaatcat tcgacaaaag acacagctaa tcacaagatt cgagctggtt ccaatgaagc
     5821 tttttatgaa tctgctgttg aatatcgcca acatgagcag gaattgcttt tgaaatacca
     5881 tactcttgaa gcacagcaaa aacgcctgca aacgttgtct gtcatgaaag atagtcctta
     5941 ttttgcccgt atcgacttta aagaagaccg tgaaacagaa acattatatc taggtatcgc
     6001 ttctttaaga gatctggcag aagaaacaat cgttattgac tggcgcgcac caattgccaa
     6061 tctttattat gaaggtgaga ttggtcccgc attttatgaa actgaaacag atagaataga
     6121 tgtcgatctg cagttgaaac gacaatttaa aatccaagaa ggacgtattt tatctatggt
     6181 agatacgtcg gaagtaatca atgatgactt tttgctagag atattggatg aatcatcaag
     6241 caaccatatg aaaaacatcg tctctacgat acaaaaagca caaaatgcaa tcattcggga
     6301 cacaacaagt aaagtaatgc tgattgaagg aatcgccgga agtggaaaga catccgcttt
     6361 gcttcaacgt attgcttttc tgttgtatca taatcgcaag tggctcgagg ctgaaaacgt
     6421 tcttctattt tcacccaacc atttgttttc tgattacatc tcgactgtat tgccttcatt
     6481 aggtgaaagc ggcgtgccta cacagacatt caaaaattat ttggtgcagc tgctgccaga
     6541 gttttcttta ctagatgaac agcaacaaga aagtggtttt ttgctagggg aaaaagatcc
     6601 gatacaagtg atgaaaagcg gattgacctt agtcgatcag attgatcgct atatccaagg
     6661 catcacaaac tacggtccct tgttccgtga tatgaaaata aatggccgta cgatcctatc
     6721 aaaggaatcg attcgccaat ggtataaaga aacaaatgaa caattacctt tacatcatag
     6781 actttctcta ttacaaacga agctcctgaa aaaattaggc ggtctgcaaa aagacgaaac
     6841 acgtcagcaa tgggtcaaag atctggcgga agaacagctc caagaactat acgcaacaga
     6901 tccaaatctt gaatatacag aacaaaagga gcgttcatta cgtaaaaaac tggccaacca
     6961 aatcgtacgt aaaagattcc gtcgaatcca tcgagggatc atgaattatc aatttgtcaa
     7021 tataacgaaa caatatctcc actttctaca aactgtgcca ctttctcttc taacacgaca
     7081 taaaatcagc gagaccaggt ggcaggaaca tttgacaact gttcgtacac acttgcgtca
     7141 aagagaattg cgtcaagagg atgctgtact attcttttta ctgatgcgcc gaacatatcc
     7201 agtgacggtt acacacaaag ctcgctatat ctttattgat gaaatgcagg atttcgcacc
     7261 atcacaagtg gcattgctgc aagccttgtt cccaaaagca aatttgactt tctgtggaga
     7321 tctaaatcaa aatgtctttg gaaatgaaac gatcgttgga tcattggaag accttttcgt
     7381 ggaccgtgaa gtcactcgtt tccaattaac gacaagctat cgctccacaa aagaaatcac
     7441 tgattttgct aatcatttta ttgtgaaaga acatcaagta gaaacaacag caagaaaagg
     7501 agaccttcct ttgctcatcc aaggaacaac agatgatatc aaattgaatt ggttagcaga
     7561 taccattcga acgactcagg gaaaagcccg gtattggcgt acagcaatca tagctaaaac
     7621 acaagaagaa tgcgaagctc tttatgctca actgccagac gagttgaaac aacaagtcca
     7681 gctgatttta aatgagagtg actttatgaa gcgttccatt attttacttc cagccttttt
     7741 agcaaaaggt ctggagtttg accgagtttt cttgtgggat gtagatgcag aacatttttc
     7801 ttctcagcaa gacaaattga tcctttacac catgtgtaca cgtgcgatgc atgaattgac
     7861 cctttttacc tccaataatg aaagtccttt gttaaacaat atggatattt catcttatga
     7921 gaaaaaagta ctgtaagtaa aaagacaact gctatcatta atgttagcag ttgtcttttt
     7981 atttacaata ataattgcag aaaaagtaat tcaatcattt aaggtcattt attctatata
     8041 taaatccaaa tctgtttcag gatattttaa tttgacaagc gaaataatca ttttcagttc
     8101 ttccatgttt tctggatatt ctgggtcaaa taccttgcta tgacggccaa tcccgccatt
     8161 gtcgatcaat cggtaaattc gattccattc aatgaattgg tcaattgatt ggaaattgct
     8221 gattggatag ccgccacaat ccaaaatctc tgatacacga aatgctaggt atgcactatc
     8281 gatcacttta ttttttttcg tgtctatctt ctcatttctt tcgataagaa ttttttcagg
     8341 acgatgtttc aagctccact ctaatagtag aaagaaagcc tccatccgat actcttcttc
     8401 aaataaccga aaggctgaat cagaacgacg aaaaagcagc ggaatcagat aattttgtct
     8461 ataaatcagc attggtgcgc ggtccaccat acactctaaa taacggttca gataccctgt
     8521 accgacatag tttcctaatc gatagccttc aacgccgaat cttgcgtatt tttgcgctaa
     8581 ttttgaacaa tcagttacgt tcacagctaa ctcttctact ttgcggtaac cgcttaagat
     8641 acgttcaaaa ggttctaaag tgatttttgc ttccatattc ttccccgtcc atcttccaca
     8701 tacttcatat catttcacat gatacttatg ctctcatcat accacaggtt gaattagaaa
     8761 agaaaataaa tcacacaaaa aagcaagaca atctgttagt gagattgtct tgcttttcga
     8821 aaaagactga atacttttcg ttaaaactgg ttgcttagtc ttctaatgcc acattatgga
     8881 acacatgctg tacatcttct agatcttcca gtgcatcaat cattttttca aatgtttctt
     8941 gatcttctgg agatagtgtt acctcgtttt gagggatcat ttggatctca gcgattgaaa
     9001 aatcagtgat tcctttttct tttaatgctt cttgtatact atggaaatcc gctacttcac
     9061 cgtaaacgat gatttgtcct tcttcttcca ttacatcgcg aacatcgatg tctttttcca
     9121 ttaggtattc aaggatctcg tcagcgtctt ctcctgcaaa agcaaagatt gctgtattgt
     9181 caaacatgta agaaaccgct ccggaaacac ccatgttccc gccatttttc ccaaatgctg
     9241 cccgtacgtc tgctgctgtt cgattcacat tgttcgtcaa tgtatcaaca atcaccattg
     9301 agccgcttgg tccaaatcct tcatagcgca attcagagta tgtttcatct cctgcacctt
     9361 ttgctttttc gattgctcgg tcaataatat gttttggtac attataagtt ttagcgcgat
     9421 caatgacgaa acgtagtttt tggtttgcat gcggatcagg atcacctgat tttgctgccg
     9481 cgtagatttc aatcccaaat tttgcataaa tccggctatt attagcatct ttagcagttt
     9541 tctttgctac gatatttgcc catttacgtc ccatattttc acttcgcttt ctgatttgat
     9601 agtttcataa cgcaaaaacg tcctgttcca ttatacttgt gaatcacgat tctgtgaagc
     9661 tttttttctt tcttccttca ttcttctcat ttttagtcaa ttatctatac tcggattggg
     9721 atttgcccga aattctgttc cggaatattg cctcccaatt gatagatttg atcacttcta
     9781 actaacagtt ccgcttcttg tgcctctttt tttccgattc gggaataaat agaaatctct
     9841 tttttttctt ttaagaatct tcttccagca tcggtaaatc caagtagtcg aagataattt
     9901 ttattttgtt ggacaggaat ctcttgcttt ttgatattca gcaagacata acaagataat
     9961 cgttggatcc gtgtccatgt atatcttttt gtttttacct ttgacacaaa atcctcaaaa
    10021 ctatcagcag ttgcagctgc tgttttcatg cgcgattcta ttccttcggt catctgatag
    10081 atatctgtca gttccagcaa agtagaagat aacaaccgat aacgcaaata cggccaataa
    10141 gattcccaag tctgcagcga acaagttgaa agatcttctt ctgtctgagg cggcacaagt
    10201 ccagctattc tttttccttc ttctaacgca tggcgaatgg ctgttgcact ggctatcctc
    10261 tgatctgtga tgattttttc atgatagcct gcacttttac gtcggatcgg atgtaattcc
    10321 atagggtgga catacttggc attttccttt gcataactta atcccaagat atgatttggc
    10381 gaagaaaaat caagcgtaat atcgggatac agactgcgaa aaacctcagt cattttttgc
    10441 ggataactat acgtttgatc agacagttct tgaaatgcct cttctatcct atcttgattc
    10501 tcttggacaa agcgtccgaa actgctgtaa tcaaacgaag tctcttcttc tgttccaaaa
    10561 cacaaactgt cacagtccag tgtttgcaag atttttactg cacctttagc aaaatagtca
    10621 gctgactgaa ccgcccattc gacgggaagc tccactacta aatccacacc attttgtaaa
    10681 gcttcattcg cgcgatgcca tttatcgatg atagccggtt cgcctctttg caaaaaattc
    10741 ccgctcatta cagcaatgac gacctctgct cctgttattt ttcgtgccat ttccacatga
    10801 tagcgatgtc cattgtgaaa aggattgtac tctacgataa tcccgcagct tttcatgcgc
    10861 atcctccttc atagttatcg ataaaaaaga aatagagaca agtaaaaaaa ggcaaaaatc
    10921 ggaaaaaata cccgaactat ataaactatt ctcgcaaaca caatacttct gcgacgaaaa
    10981 gctgcactgc aagagcaatg cctatatcgt tcggatatat aatttatgtt gaagatttat
    11041 tcttttcttg ttaactcgag caggcaaaac cgctagtctt tgtatgctac aaagaaccag
    11101 cgctttgatt tctctgatgc tgcttcgtct gtaaaatcag aaaatgcttc gactttcgaa
    11161 aagccgctgt tttccagcat tcttaaataa ctttccaatg gataggtacg ctcttgatgt
    11221 agttcatctt cacgaataaa tcgaccagac ccttcttgtt cttcaacgaa aaaggtaaga
    11281 aaatgttcga tgctgtgttt ctcgcttcct ggataactat cccacaaaaa agcaaaatta
    11341 tccccttgat aatgatagct atattctgga aaaacctcat ctacttgata ggtggagtgt
    11401 acgtcaaata aaaatcggcc attttcttct aacgcttgat agacttcatc aaacacttgc
    11461 tgtacctctt gtctattttt catatagcac agcgaatcag aaaaacaggt aattgcctga
    11521 tattgtccga tttctgataa atcgagcata ttgccttcca caaattggat cgatgcgtct
    11581 tcttcatatg ctcgctggct ggcaatcatc aacatctctt ctgaaaggtc taaccctgtc
    11641 actttatatc catcttttgc aaaggcaacc gctaatgctc cagtaccgca tgccagttcc
    11701 aaaatatttt ttgtgtcttc ttgtaaatga cgtttcgaaa aagctaacca ttgctgataa
    11761 agtgtttcgt ccatcacttc atcgtaaatg aaagcaaatg tttcataagc catatgttaa
    11821 tccaaccatt cactaagatc gactagcgga gcatctgacc atagtttttc cagattatag
    11881 aaatttcgtt ctggcgcatg gaaaacatgt acgatcacat ctccaagatc gatcaatacc
    11941 cattttccgc cttcttttcc ttcaatacgt tttacttcat atttgttttc ttcttcttta
    12001 tcaatgattt cttctgtaat tgcgttgatt tgacgttcgc tatttgctga acagatcatg
    12061 aaataatctg ccaacaagga aacatttcga acgtctaaag cgataatgtc ttctgcccgt
    12121 tttgaatctg ctgcttcaat cgcaattttt aattgttctt tactatctat gatgatttcc
    12181 tcctaatatg agcagcttat ttttttgcta cccagtaatt atatgtttcg attgtttttg
    12241 gataaatctg ttgttcttgc gcaagcaaat gggccaatgt atgttttgtt tcaaatgcta
    12301 cagcttcgtc tagatcagcc catgcaatgg ctcgtgcatc ttgtacccca ggaaaatctc
    12361 gacctggttc aatatagtct gctacataaa tgattttgtc aaggagactc atcttagcag
    12421 ccccagtcgt atgcacccgg attgcatgaa gaatctctgc atcggtgatt cctaattccc
    12481 gctctacaaa cgaagcgcca acaagaccat gccagattgc attattataa ttcaataact
    12541 ccggatcaaa accgtctctt acgattacca ttttgaactc ctcatctggt cgttcttttg
    12601 cataatcatg tgtcaatgca gcaatactag ctttttcagg agaagcaccg tattttttgg
    12661 caagagccac ggctgtttct tctactccaa gcacatgctt gaagcgttgt tcgctcattc
    12721 tcatctgtat tttttgcatc aggttttctc tagaataagc agtatattca ttactatagt
    12781 ccatattcat acaacccttc attttgaata taatcaatta ctttatctgg aacaaggtag
    12841 cggatagaac agccttcttt gatcttttga cgtattttcg tggaactgat gtcgatctca
    12901 ggtacatcta cccaaattac aggatatgga gtatccgttg tgtatcctgg tcgacgtata
    12961 cccacaaagt tgaccataga agtcagctca tcgattttat accattttgg caaatattct
    13021 accatatccc caccgataat aaagtaataa tctgtatccg gattgttttg cgtcaattcc
    13081 ttcatagtat cataggtgta acttttccca ccacgagcta attcaatcgt ttcaatctgc
    13141 aaaaaaggat tgtcttccac cgctaattcc aacatgttga gtcgatgttt tgcatcaatc
    13201 gtctgtttct catcaacatg aggtggctga taagaaggca tcaaaaatac gcggtcaaga
    13261 cctaaattcc ggccagcttg ttcagccatc actaaatgtg caaggtgaac gggattgaat
    13321 gttccgccca agatccctac ttgctttttc ttctctaaga attgaggcat ttcttcagga
    13381 aataattgtg aacgaacaaa tgtcttagct tgcgtattca tcatggtcac tccttatatt
    13441 gcttttactt cttttgatag cctttgatac ttttcttttg tggaaggttt gtataataca
    13501 ataacacgtc caatgatttg gacgatttcg caatctattt ctttttctaa tacttgagct
    13561 gcttcttctg caatctcatc tgtattttga agtaacgata ctttgatcag ttctcttttt
    13621 tctaaagctt cttcaatttg aacgatcatt gctgaattga tcccaccttt tccgatttga
    13681 aaaatcggct gaagatgatg cgctttgcta cgtaaaaaac gcttttgttt tcctcttaaa
    13741 ctcataattt tccttcttcc ttaaatcaat gctttgcgtt tcaatacatc tacacccttt
    13801 ggcgcccagc ccgcaattac tactggcgct gttacagtga tccagcctag accggcaaaa
    13861 acgatgtctg ttttttcttt cacagaaaac tcaaaacgaa caagctccgg aaatgcctcg
    13921 acttcatcag gacgaggagg ctgtaataat ccacctacat gtttttgata aaattcgtct
    13981 gctttttcca gtttcgttcg atgaatattt acatcatttg acacataagc aataaatgaa
    14041 gcacgattgc cgctgataaa atcaaatctt gcaagtccgc ccaagaaaag tgtttgccct
    14101 tcattcagct gataaacttt aggtttgatt tcttttaacg gtgcaatgat ccgcaaatct
    14161 tttttaccta agtagtgcgc catttgatgg cgatggataa ttcccggtgt atcaatcaaa
    14221 aaatgcccgt catctaaagg aatctcgatt ttatccaagg tcgttcctgg aaactgcgaa
    14281 gttgtgatta catcttgtac tcccgctgta tttttgatga tttgattgat aagcgtcgat
    14341 tttccgacat ttgttactcc gacgatatag acatcccgtc catcgcggta ttcttcgatt
    14401 ttttctaata gttccgtcat ctcatgagat ttcttagcac tagttaataa tacatctact
    14461 ggacgaattc ctgcttcgta agcacgttcc ttcatccatt gtgttaattt agtttttttc
    14521 aatgatttag gtaatatatc cactttgttc ccaactaaaa gtactggatt gtcaccgaca
    14581 aaacggtgta gtcctgggat tagtgaacca ttgaagtcaa aaatatccac cacattgacg
    14641 atcaatgcat ctttttgccc taattcattc agtaagcgca aaaagtcgtc atccgtcaat
    14701 gagacatctt ggatctcatt ataatggcgt aatctgaaac aacgctgaca atagacttcc
    14761 ccggaagcta aacctttttc caaagccgtt tgcggcgtgt atcctaattc accgggtttg
    14821 tttgtttgta taacggcacc acagccgata cattgtaatt cttctgtcat tcgattccac
    14881 ctcgccagcc catatcaggg tgttttgcta atagataggc cataattctt ttttcccaaa
    14941 aacgattaaa ttgtgtcttc caactatctg ttgcgataat gggttttaca aggatgctac
    15001 gtatccctgc acgattggct ccacgtatat cagtcataat ctgatcgcct accattacta
    15061 tctcatcatc tgctaaatcc aattgtttct ttgctatgtt gatccctctt gctaaaggtt
    15121 tcattgctcg tgcaacatac aaaagatcaa atttttcaat cgctcgcgcg acacgttttg
    15181 aattattgtt tgaaacaaca acaacaggga ttcctgcatt tttcatttct aaaatccaag
    15241 taagcaactc ttctgttcca tctggattat tccatgcaat caacgtattg tctaaatcgg
    15301 tcaatactgc tttgatgcct agattcttta attgggcagg tgtaatctga tagatggctt
    15361 ctaccatcca tgttggtttg taatttgaaa acatgctgtc tcctcatctg caaaaaatga
    15421 aggagcacat aaagtactcc tcaaccccgt attatactgc atatcagcaa aaaaaactac
    15481 ctctatactc gttcgtcttt cagaaaaagc atttctttac aaaatgacca aaaataaaga
    15541 ttgatcacag aaaaatttag aaacaaaaaa caactaatat tggcggaacc tcttgtatcg
    15601 tttttccagt aatttatgct gatctaatcg aacaagttcc gtcaatcttt caatcaacgc
    15661 ttttttcata atgtgtgcga ctttatatcg tggcaactgc tcgccgttga acgtttcagg
    15721 aataactcga tcaacgattc ctaactcgcg tagttctggc gcagtaattt tcatcaactc
    15781 agctgcttct gcggcgcgac taccgtcttt ccacaaaatc gacgcaaatc cttctggaga
    15841 aagcacagca taaatcgttt cttccagcat ccagacttca tccgctaaac ctagagcaag
    15901 ggcaccgcca cttccacctt caccaatgat gatggcgatg atcggtacct ttaaatcaga
    15961 catttcgagc agatttctgg caatagcctc tccttcgccg cgctcttctg ctcctacacc
    16021 gcaatatgca cctgctgtat tgatcaaggt aatcaccggt ctattgaact tttccgcttg
    16081 tttcatcagt cgtaaagctt ttcgatagcc ttcaggatgg ggagaaccaa aatttctgcg
    16141 gatattttcc tgcagatccc gtcctttttg aataccgatc actgttaccg gtttgccatc
    16201 taatcgagcg attccaccaa caatcgcttc gtcatctgca aataaacgat caccatggca
    16261 ttcctcgaaa gaatcgaaga tctcttcaaa aaaatccaag cttgtccatc gttctttagc
    16321 tcttgctaac tgaacgacat catgtgctgt cttatccacg atctatccat cctttctgtg
    16381 tatgcaattc aagaagcgta tggattttct gtttcaattc catgcgggga acaatctgat
    16441 cgacaaaacc atgagataat aagaattcag ccttttggaa atcttctgga agttcctgct
    16501 tgatcgtctg ttcgatgacg cgtcttccag caaaaccgat caagctttgg ggttctgcta
    16561 gaataatatc gccttccatt gcaaaactgg cagttactcc tccggtcgtc gggtccgtta
    16621 aaacagttag ataaaaaaga ccttcattgc tatgccgttt gacagcagca gagatttttg
    16681 ccatctgcat tagagaaaag attccttctt gcatccgtgc gccacctgaa gctgtgaaca
    16741 agacgactgg aagactttcc tttgttgctc gttcgaataa tctggtgatc ttttctccga
    16801 caatcgttcc catacttccc atgataaaat taggatccat cactccaata atgaagggaa
    16861 tcccctgaat ggttgcttta ccagtgagga ctgcttcatg aagtcctgtt ttatcttgca
    16921 tttttttaat tttttccgta tattcgggaa atgctagcgg atctttggtt acaagatccg
    16981 tatcccattc ttcaaaactt ttttcatcaa taatcaaagc tagacgctgc catgcaccga
    17041 ttcgaaaagc gtatccgcaa tgagggcata ctttttcttc accgatgtct ttcgtataaa
    17101 ggatatgttt gcaatttgga cactttgccc acatgttatc aggaacagat ggcgagttag
    17161 caggtttgct ctctctatta ggattgatcc gaatatattt ctttttctta aacagagcca
    17221 tctatgcgcc tcctatctcc gtttctggtg tccaattcgg taaaaattct tcttgtaaaa
    17281 atgctgtgct gtaatcacct gctatgactg caggatgact aatcaaatcc atctgaaatt
    17341 ctgcgttcgt gatgattcct tctgtgataa attcacttaa ggctcgctgc atcttcatta
    17401 atgcttcaaa acgcgtattt ccatggacga ttatttttgc aatcattgaa tcataatatg
    17461 gcgggatcgt atatcccggg taaactgcac tgtctactcg cagtcccatc ccacctgctg
    17521 gaagcagtaa gttttggatc ttccctggag aaggagcaaa atggaaagca ggattttctg
    17581 cattaatccg gcattcgatt gcgtgtcctt ggaatactac ttcttcttga cacacattca
    17641 aaggctcacc ggctgctatc tccacctgtt tttttactaa atcgattcct gtgaccattt
    17701 ctgttactgg atgttccact tggattcgag tattcatttc catgaaataa aaatcgcctt
    17761 cttcatccat cagaaactcg atcgttccgg cattttcata attgactgct ttagctgctc
    17821 gaacagcagc gtctcctagt gcttctcttt ttgtttgtga gatcactaca gaaggagatt
    17881 cttccagtac tttttgattg ttgcgttgta aagaacaatc tcgctcgcct aaatgaatga
    17941 catgaccaaa tcgatcacct aagatctgaa cttctatatg gcgagcagga taaatgattt
    18001 tttccaaata catatcatca tttccaaatg ccgctgctgc ttcttgctga gctgaagaaa
    18061 aatgctgtgg gagctcttct tttgattgaa ctttccgaat gcctttcccg ccaccacctg
    18121 cagctgcttt cagcatcact ggataaccaa ttctgtctgc tatttccaat gcttcttcta
    18181 ctgtagataa tacaccagta cttcctggaa tgactgggac atttgcttct tgcatcaatt
    18241 gacgcgcatt gatcttattt cccattgcat cgatcgttgc acttttagga ccaataaatg
    18301 tgatattgca ctcttcacac atggaggcaa atcggctatt ttcagataaa aaaccaaatc
    18361 ctggatgaat tgcttcggct tttgtcacga ttgcagcgct cagtacttcc tgaacattca
    18421 gatatgaatc aacggctttt gccgggccaa tgcagattgc ttcatctgct aactgtgtat
    18481 gaagtgcttc ggcatctgct tctgaatata tcgctacggt ttggatccct agttcgcggc
    18541 atgcacgaat gatacgtaca gcgatttcac cgcggttagc tatcaatact ttagaaaaca
    18601 tatttcacct atccaatcat aaaggttaat tccgcttcgg ctactttttt accgtctact
    18661 ttagcgattc ctttgcctat tccggctgaa gctttcactt tcaatatctc aacttcaagg
    18721 tataaggtat ctcccggtgt aactttctta cggaattttg ctttgtccaa tccgccgaaa
    18781 taagccgtct ttcccttgaa ttgttcaaga gaaagtaatg caactgctcc tgcttgagcc
    18841 attgcttcaa caatcaatac ccctggcata acaggttctt ctggaaaatg tccttgaaaa
    18901 aagggctcat tgatcgttac atttttctta gcaacgatcc gttctccttc aaccatttct
    18961 tccactcgat caatcaaaag cattggataa cgatgtggaa tgatttcttt tatttcttgt
    19021 atgttcattg ttttccctct ttcctgcttt gcttattttt gctgaatcct aaagagtggc
    19081 tgtccatatt cgacaactga ctcgttttct acaaggattt ctgtgatcac tccgtcggcc
    19141 tcactagtta tctcattcat cagtttcatc gcttcaataa tgcagataac ttctcctttg
    19201 gcaacttgat cacctacttt tttgaaagct ggtttgtcgg gcgctggttg cagataaacg
    19261 attcccacga ttggcgaaac aacctcgatt ccatcaaata ctgccgattc tttttctttc
    19321 attatttctg gagcagttgt tgcttgttca gatatgacat tgactggctc cgaaactggc
    19381 aacgtttcgt ttgtttgaac catattcatg cctcgacttg ttttattttt attcatatac
    19441 aattcaaatt gaccttcacg caaatcgaat tccgttagcg aggattgatc aaattgactg
    19501 actaattctc tgatttcatt gatatccaaa attattcctc ccagcgtttc atgcacagaa
    19561 ccgcattatg tccgccaaac cctaatgaat tactcattgc ataagtaaaa cttgcaggtt
    19621 tgctttcgtt tttgacaact gtcaagtcaa tctcaggatc ttgtttttct acattgatcg
    19681 taggtgggat aaattgatgt tctaatgcca gcaaagttgc caaggcttcg atcccgccgg
    19741 ctgctcccaa taaatgtcca gtcatcgatt tcgtgctgct gacgtaagta tctctgaaat
    19801 tttcacctaa tgcgatttgg attgcttttg cttctgcctg gtcattggct ggtgtacttg
    19861 ttccgtgagc attgatatat ccgacttgtt ccggctgtac accagcttct tccatcgcaa
    19921 gcttcattgc tttagatgca ccagaaccat ctggagtagg agctgtcata tggtaagcgt
    19981 cacagtttgc accgtatcca acgatttctc catagatttt ggcaccgcga gcttgtgcat
    20041 gttctaaaga ttcaagaact agaacgcctg ctccttcacc catcacaaaa ccattccgat
    20101 tttcgtcaaa cggaatcgaa ccacgattag gatcagttga ttctgagagt gctgttaaag
    20161 ctgcaaatcc agcaatcccc atctcacaaa tcgttgcttc tgttcctcca gcaagaatga
    20221 catcagaata gccatgtttg atgttacgga atgcttcccc aattgaattc gttccgctag
    20281 cacaagctgt tacgatagct gtacaaatac cttttgcacc tacacgcaaa gcaatatttc
    20341 cagctgccat gttggcaatc gccattggta cgaacattgg agaaactcgt tttgcacctt
    20401 tttcatgtag acgaataact tggttttcga ttgttggaag accaccgatc ccagatccga
    20461 tgatgacgcc aaaccggtct tgattcattt tttctttatc taaaccgctc atctccattg
    20521 cttctaaagc agcgtagatt ccatagatcg aaaacatatc cattcgtttg ctatcttttt
    20581 taataaaata tttatcgtaa gggaaatctt tgacttcacc cgctaaggta atccctgttt
    20641 ctgaagcatc aaatttagtg atcggtgcga tgccattcgt cccctctttt aagctattca
    20701 aaaagctgtc tgcgtcattt ccaataggtg aagtgactcc atagccagta attaccactc
    20761 gattcatttg tttgctctcc ttttcttatc cgtgcatgac caatccgcca tcgacattga
    20821 tgacttgtcc ggtgatatat ggattttgtg ctaagaaaac tgctgtttgt gcaatatctt
    20881 ctacttgtcc gaaacgctgc atcggaattt gcttttccgc ttgttccttg actttgtctg
    20941 ctaaaacttc cgtcatatca gttgtgatga atcctggcgc aatggcatta catgtaatgc
    21001 ctctagttgc agcttctcgt gccacagatt ttgttaatcc tactacgcca gctttactag
    21061 ctgcataatt tgcttgacca atatttccaa tcaacccaga tacactagag agattaatga
    21121 tggccccttc tcgctgcttc atcatttttt tcaatacgtg ctgcgtcata ttgaaggttc
    21181 caatcaaatt gatatccaga cattttttga aatcttctgc atccattcgc atcacaagtt
    21241 tgtcatttgt gattccggca ttgtttacta acacatggat cgatccaagc ttttcttctg
    21301 cttctttgat catttgtccc gctttctcat aatcagagat gtcaccggaa actcctacgc
    21361 attttacacc gaatgcttcg atttcttcta tcttttcttt tggaatctct ccgcgaccat
    21421 tcaaaatgat atttgctcca gcttttgcaa aagctaaggc catcgctttg ccgattcctc
    21481 ttgtactacc tgtgataaat acattttttc cttttacatc cataacgttc ctccttatag
    21541 tattgctatt gtttcatcaa atgtctgctt attctcaaca cgcagaactg gtattgtttt
    21601 atcgattttt ttcataaatc cgcttaatac ttttccgggg ccgacttcga tgacttgttc
    21661 gatgccgatt gtttttaact tatggatact ctcatagaaa cgaacaggtt gcatgacttg
    21721 ctgttcaagt agtcccgcga ttgtttcttt cttcattatt tccgttgttg tgttgcttat
    21781 gataggaaaa gaaggttcag agaattgaat ctgacttagg tccttcgcca gtttttttgc
    21841 tgccggttct aaaatagctg tatgaaaagg cccgctgaca tttagaggga tcattctttt
    21901 gaaccctttt tcttttaata gtaaaacagc ttcgtctact gctttttctt cgccaccaat
    21961 cacgatctgc tgaggtgtat tataatttgc aggagatacg atgccgtact tgctagcttc
    22021 atgacagctt tcctcgatcg tttcaatcgg cgcattcatc actgcaacca ttttcccgct
    22081 tccagctggg gtagcttctg tcatataagc gcctcttttt gctactaacg caactgcttc
    22141 agtgaaggat agcgcattac tagctactaa tgccgaatat tctcctaaac tcagtcctaa
    22201 agctgcatct gggatgattc catgctcctt caataagcga taatacgcga tgctgactgt
    22261 tagaatagct ggctgtgtat attgcgtctg atccaaacgt tcattttctg taaagcaaag
    22321 ttcagccata tcgtagccga gaatctcact tgcttcatca aatgtctgct ttacaacggc
    22381 ttcttcatat aaatcttttc ccataccttg atattgtgca ccttggccac taaataagaa
    22441 tgctgttttc atgtttttct ccaatatatc cagaatatat gtttgattct tcctattcaa
    22501 aaaggccgag acattcctat ttcaggaaat gtcccagccc ccaacctaat tttaatgatt
    22561 atgcagtttg tttttctacg taagtcacta aatcttgaac cgttttgatt ccgtcttctg
    22621 tttcgatttt cacgtcaaat tcatcttcaa tttcattgat gatttggaat aaatccaagc
    22681 tgtcagcttc taagtcttct tggatgtcag tagtcaattt gatttcttca gtttcttttc
    22741 ctaattcttc tgcgatgatt tcttgaattt tttcgaatac cataataata tattcctccg
    22801 atattgtttg ctttttattt ttataattta acagtctaca actccatgag aatacttccc
    22861 catgttaaac ctccgccata gcctgtaaat attactttct gctgttttcc taaggtgatc
    22921 tttccactag aaactgcttc atccaataag atagggatgc ttgcagcaga tgtattccca
    22981 tatttatcca tattggtcag gaacttttct tgcggcatct ttacttttcg ggcaattttc
    23041 tcaataattc tgaggtttgc ctgatgcaaa agcagataat caactggttt gtctttcatg
    23101 acttcttgga tattgtttgc aacgtctcgt acggcaaaat caaaaatatc tctgccagtc
    23161 atttgcaaat aatacgaacc ttctgaatct tctgaataga acggattttt attttgctgg
    23221 tagccagcgg tcaatgagct acttctggta ccgtcagctg ctaatttttc tttcagcaat
    23281 tttttctgtg gattcgcttc taagagaatc ccacctgctc catcgccaaa caacacagca
    23341 gtcgtacgat cagaccaatc gattagctta gatagtgttt caccaccaat caccagtccc
    23401 ttagttgagc cgcagcggat cattttttca gccatgctta acgcataaac aaatcctgag
    23461 cacgcagcac tgatatcaaa agcgatagca tgagttgctc ccaatttccc ttgtacttgt
    23521 gctgctacag atggtgtcgt gtaatcgggc gacattgttg ctactaaaat aaaatccaaa
    23581 tctttagggt ttagattttt ctgttttatc aatttttccg ccactaaaat gcataaatca
    23641 gaagtctcct gatttgttac acatcttcgt tcacgaatac ccgtccttga cgaaatccat
    23701 tcatcagagg tatccatcat cttggaaaga tcattattag taataatctt ttcaggaagg
    23761 acgctggctg ttgcagtaat cattccaaat tgttccatta aatatcctca cttatagtct
    23821 tgcaagaagt cgtgcaagtt tttcagtgca tccagcaagg catgctgttc ctccgtagcc
    23881 attccgtcta agatgttctt gaccatttca cgatgaaaat gctgatggac ccgaaatagt
    23941 aactttcctt ttttcgttag tcccaacttt acaacgcggc ggtcatcttc actgcggata
    24001 cgttcgacat agcctttttt tactaacttg ttaatagcag tggtcaaggt cccaacagta
    24061 attgatagtt ctttggctac ttctgaagtt gtttttttct tgtacatccc gattgcctcg
    24121 atcgtgtgca tctctgtgat tgacagatct ttgaattgag attttttcag ctcagactct
    24181 tcgattgtca aaatatcatt gaagacacta acaagataat catttactgt ttttaaatct
    24241 ggttccatgt tccaccgtcc actaattagt ttgattatca aatcttttga tgttcaaact
    24301 atatcttaac ttttttcaaa atgcaacctt aaaagatcat ttttttacta t
//
LOCUS       DAXLPI010000032        23846 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXLPI010000032 DAXLPI010000000
VERSION     DAXLPI010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.032"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(55..789)
                     /locus_tag="ACF3T5_001124"
     CDS             complement(55..789)
                     /locus_tag="ACF3T5_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFD3631747.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="ACF3T5_001125"
     CDS             1116..1526
                     /locus_tag="ACF3T5_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFD3631748.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="ACF3T5_001126"
     CDS             1519..2220
                     /locus_tag="ACF3T5_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFD3631749.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="ACF3T5_001127"
     CDS             2220..3806
                     /locus_tag="ACF3T5_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3631750.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="ACF3T5_001128"
     CDS             3825..4046
                     /locus_tag="ACF3T5_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFD3631751.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="ACF3T5_001129"
     CDS             4043..4804
                     /locus_tag="ACF3T5_001129"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFD3631752.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="ACF3T5_001130"
     CDS             5184..5693
                     /locus_tag="ACF3T5_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFD3631753.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="ACF3T5_001131"
     CDS             complement(5768..6307)
                     /locus_tag="ACF3T5_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFD3631754.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACF3T5_001132"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="ACF3T5_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFD3631755.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7320..8171
                     /locus_tag="ACF3T5_001133"
     CDS             7320..8171
                     /locus_tag="ACF3T5_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFD3631756.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8199..8834
                     /locus_tag="ACF3T5_001134"
     CDS             8199..8834
                     /locus_tag="ACF3T5_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFD3631757.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="ACF3T5_001135"
     CDS             8854..9627
                     /locus_tag="ACF3T5_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFD3631758.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="ACF3T5_001136"
     CDS             10035..10832
                     /locus_tag="ACF3T5_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFD3631759.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="ACF3T5_001137"
     CDS             10810..11223
                     /locus_tag="ACF3T5_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFD3631760.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="ACF3T5_001138"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="ACF3T5_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFD3631761.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="ACF3T5_001139"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="ACF3T5_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFD3631762.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="ACF3T5_001140"
     CDS             16000..16560
                     /locus_tag="ACF3T5_001140"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFD3631763.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACF3T5_001141"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="ACF3T5_001141"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFD3631764.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="ACF3T5_001142"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="ACF3T5_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFD3631765.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="ACF3T5_001143"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="ACF3T5_001143"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFD3631766.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="ACF3T5_001144"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="ACF3T5_001144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFD3631767.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="ACF3T5_001145"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="ACF3T5_001145"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFD3631768.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="ACF3T5_001146"
     CDS             21907..22485
                     /locus_tag="ACF3T5_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3631769.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="ACF3T5_001147"
     CDS             22537..23019
                     /locus_tag="ACF3T5_001147"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFD3631770.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="ACF3T5_001148"
     CDS             23136..23510
                     /locus_tag="ACF3T5_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631771.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7787 a         4288 c         5119 g         6652 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcaccggg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatggaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaac aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcacagca tgacagcatt
    23641 gggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cactagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt ttttgcgaaa actatt
//
LOCUS       DAXLPI010000033        23709 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXLPI010000033 DAXLPI010000000
VERSION     DAXLPI010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.033"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(10..129)
                     /locus_tag="ACF3T5_001149"
     CDS             complement(10..129)
                     /locus_tag="ACF3T5_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFD3631772.1"
                     /translation="MVFQKPLNNFRYIVYYCHTKIKKNKQKETVTKVLSQSLI"
     gene            308..1969
                     /locus_tag="ACF3T5_001150"
     CDS             308..1969
                     /locus_tag="ACF3T5_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFD3631773.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2039..2386)
                     /locus_tag="ACF3T5_001151"
     CDS             complement(2039..2386)
                     /locus_tag="ACF3T5_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFD3631774.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2470..3411
                     /gene="opp3b"
                     /locus_tag="ACF3T5_001152"
     CDS             2470..3411
                     /gene="opp3b"
                     /locus_tag="ACF3T5_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFD3631775.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3412..4455
                     /locus_tag="ACF3T5_001153"
     CDS             3412..4455
                     /locus_tag="ACF3T5_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631776.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4461..5531
                     /locus_tag="ACF3T5_001154"
     CDS             4461..5531
                     /locus_tag="ACF3T5_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631777.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5521..6474
                     /locus_tag="ACF3T5_001155"
     CDS             5521..6474
                     /locus_tag="ACF3T5_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631778.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6486..7496
                     /locus_tag="ACF3T5_001156"
     CDS             6486..7496
                     /locus_tag="ACF3T5_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFD3631779.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7781..8602)
                     /locus_tag="ACF3T5_001157"
     CDS             complement(7781..8602)
                     /locus_tag="ACF3T5_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFD3631780.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8699..8977)
                     /locus_tag="ACF3T5_001158"
     CDS             complement(8699..8977)
                     /locus_tag="ACF3T5_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631781.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9245..11254
                     /gene="metG"
                     /locus_tag="ACF3T5_001159"
     CDS             9245..11254
                     /gene="metG"
                     /locus_tag="ACF3T5_001159"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFD3631782.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11733..12320)
                     /locus_tag="ACF3T5_001160"
     CDS             complement(11733..12320)
                     /locus_tag="ACF3T5_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631783.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12495..13817
                     /locus_tag="ACF3T5_001161"
     CDS             12495..13817
                     /locus_tag="ACF3T5_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD3631784.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13972..14748
                     /locus_tag="ACF3T5_001162"
     CDS             13972..14748
                     /locus_tag="ACF3T5_001162"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFD3631785.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14741..15310
                     /gene="rnmV"
                     /locus_tag="ACF3T5_001163"
     CDS             14741..15310
                     /gene="rnmV"
                     /locus_tag="ACF3T5_001163"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFD3631786.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15303..16187
                     /gene="rsmA"
                     /locus_tag="ACF3T5_001164"
     CDS             15303..16187
                     /gene="rsmA"
                     /locus_tag="ACF3T5_001164"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFD3631787.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16638..17267
                     /locus_tag="ACF3T5_001165"
     CDS             16638..17267
                     /locus_tag="ACF3T5_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631788.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17391..18500)
                     /locus_tag="ACF3T5_001166"
     CDS             complement(17391..18500)
                     /locus_tag="ACF3T5_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631789.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18709..19131
                     /gene="mgsA"
                     /locus_tag="ACF3T5_001167"
     CDS             18709..19131
                     /gene="mgsA"
                     /locus_tag="ACF3T5_001167"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFD3631790.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19318..19416
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19454..19990
                     /locus_tag="ACF3T5_001168"
     CDS             19454..19990
                     /locus_tag="ACF3T5_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFD3631791.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20030..21793)
                     /locus_tag="ACF3T5_001169"
     CDS             complement(20030..21793)
                     /locus_tag="ACF3T5_001169"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631792.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21796..23511)
                     /locus_tag="ACF3T5_001170"
     CDS             complement(21796..23511)
                     /locus_tag="ACF3T5_001170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631793.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7648 a         4331 c         4807 g         6923 t
ORIGIN      
        1 accgtttatt cagataaggg actgtgacaa gacttttgtc acagtttctt tttgtttatt
       61 cttttttatc tttgtatggc aataatagac aatataacga aaattattta atggcttttg
      121 gaagaccatg gaaaaccttg tacaaagcta ttttttctga aaaattcctt gaaacctcac
      181 gaaaaaatca ttattttatg aaaaagacaa aaaaaagtgt tgatttttta ataattattc
      241 attaaactct taaacaactt cagaaaatat taattattct gaaaattcca ataaaggggt
      301 acgtaaaatg aaaaagaaat ttacatttgg tttggttgcg gtatgcggcc ttgtacttgc
      361 tggatgttat ggcggcagtt cagccacaag cagtagttca gcaaaaagcg gttcagcaga
      421 tggaggaggc gtatttaact tagtcgtgcc tcaagaaatg cctacagctg acttatcagt
      481 tgcaacagat acaatcagtt ttacagcatt gaataacgtt tatgaaggaa tttaccgtct
      541 tgatgaagac agcaaaccac agccggcagg agctagcgag ttggcagaag tcagtgaaga
      601 cggattaact tacaaaatca aattacgaga agacgcaaaa tggtcaaatg gtgatcctgt
      661 tacagcagcg gattacgttt atggctggca acgaacagta gacccagcta cagcttctga
      721 atatgcttac ctgtatgcgc cagttgaaaa tgcagaagac attacagctg gaaagaaaga
      781 taaatcagaa ctagggatca aagcagttgg tgactatgaa ttagaaatca aattgacgaa
      841 acaaacacca tatttccaat atctgcttgc tttcccatca tttttcccac aaaaacaatc
      901 cgtagtggaa gaaaatgggg atgcctatgc atctgcaagt gataaagctg tttataatgg
      961 accattcaca ttagaagatt ttgatggacc aggaacagat acagaatgga cttataagaa
     1021 aaatgatcat tactgggaca aagatacagt aaaactttct gaaatcaaag tcagtgtagt
     1081 aaaagaatct tctactgcat tgaacttatt caaagatggc caagcagatg acgtgatctt
     1141 gtctggtgag ttagcacagc aaaacgcaaa cgatccagct tatacttctg ttaaagaagc
     1201 gcggacaagc tatatcgaat tcaatcaacg tgaaaagaat tcaccattca acaatgtgaa
     1261 cctaagaaaa gctatttctt attctatcga tcgtgaagct ttagtaaaac aagtactagg
     1321 tgacggatca gttgtctcta caggattgat cccatcagat atggtaaaaa gtccaacaac
     1381 gaacgaagac tttgcaaaag aagctggaaa attagtttct tatgacaaag ataaagcaaa
     1441 agaatattgg gaaaaagcga aaaaagaatt gggcgttgat tctttagaat ttgatttgat
     1501 ggcttctgat gatgattcat cgaaaaaagt aatcgaatat gttcaaaact caatccaaga
     1561 aaacttggat ggcgtaacag tcaaaccaac acctgttcca ttctctgtac gtcttgaccg
     1621 ctcaacttcg ggtgattttg acgcagtact aggcggatgg gctgctgact atgcagaccc
     1681 aagcagcttt actgatttgt ttgtcacagg taattcatac aaccgtggtc gctggagcaa
     1741 tgcagaatat gacgaagcag ttaaagcagc ttcatcaaca gacgctggcg atgaacaagc
     1801 ccgttgggat gatttccaaa aagcaatcaa gatcatctct gatgatatgg gtgtcgttcc
     1861 ggtgtaccaa aaagcagaag gtcacctagt gaatgaaaaa atcaaaggta tcgttcacca
     1921 tgcggcaggc gcttcatggg attacaaatg gacatacgtt gaagaataat cgttaatacc
     1981 gtgagcatga gaaaaaacga ggctgcggca aatgtcgcaa cctcgttctc atattcactt
     2041 atcctaacaa agaaaaaagc atgaaaatag cagaaagagc aattagtatt ccggcaatga
     2101 tcagccaaaa gctgtacata ccgtagctgg cagacgagag cgaggaaaat tctggttttt
     2161 tctttcggtt ccttgttcgt gcaagatgga ccgatcgctg aaaatgatcg aagaaacccg
     2221 aagaaaagac ccataaaaaa ccgccgataa tcagaaacgg caatgaccat aagaatagat
     2281 cattagataa ttgaatgagt gtaaaggaat ggttgatgag attttttatt aaaataacca
     2341 gaatcagtag aaaagctact gcgtagggga tagatttttt tttcatttga ttgctccttt
     2401 atttttggtt cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg
     2461 gaaacattag tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc
     2521 acgctttggt taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca
     2581 tatacgaatc aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt
     2641 ttggataaac cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat
     2701 tttggaattt ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc
     2761 ggaccatcct tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt
     2821 ttaggaacga tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta
     2881 gcgatattag gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt
     2941 gcaatcaaat tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta
     3001 ccaacaattg cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa
     3061 atggtggaag tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt
     3121 tgggaaatcg cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta
     3181 ggaccgttag ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt
     3241 cctggtatcg gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg
     3301 gctgtaacga tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg
     3361 tacggtctag ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg
     3421 ataccaaagc gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa
     3481 aaaaataccg aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat
     3541 tcatggcgcc gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc
     3601 atcattttga tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac
     3661 gtggcttata tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga
     3721 aaagcaatgg ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac
     3781 gtaaatttct ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg
     3841 ggaatgagaa tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt
     3901 gtagcttacg ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt
     3961 tttttggaaa tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt
     4021 tttgaaccag gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg
     4081 gcacgtatcg ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga
     4141 ttgacattag gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct
     4201 agtgtcatta ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt
     4261 ttaagcttta tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc
     4321 gaagggtaca agacattcct gtatctgcct tatctattat ggattccggc agtaacttta
     4381 tcggttatca tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct
     4441 aaaatgaaag agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt
     4501 ttgatacgta tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag
     4561 gagaaacatt ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca
     4621 tcatgcgctt gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta
     4681 acgatatcgt tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga
     4741 tgattttcca agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg
     4801 ctgagtccct tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag
     4861 aattgcttaa attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc
     4921 agttctcagg aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg
     4981 aaatcctgat tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt
     5041 tggagctgct gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg
     5101 accttggtgt agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg
     5161 ttgaagttgg aacatcggaa gaaattttct acaacccgca acatccctat acttggggac
     5221 tgcttggttc aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt
     5281 ctccaccgga tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg
     5341 cgatgaaaat cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg
     5401 cagcaacttg gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac
     5461 gtcgctgggc tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg
     5521 atgccaaata aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag
     5581 aatgaagtac atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt
     5641 ttagttggag aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat
     5701 gaacctactg atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa
     5761 aaaggtttga cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg
     5821 ttgaatcctc gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta
     5881 gctaaatcat ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg
     5941 aatccaagcc acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt
     6001 ggtattgcac gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct
     6061 gcactagatg tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa
     6121 catcaattga cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac
     6181 cgaatcggtg taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat
     6241 aatcacggtg ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca
     6301 gaccatgaga gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta
     6361 cgtcaacttc gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca
     6421 tattacgcaa aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga
     6481 catagatgct aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat
     6541 tgattcgtac ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga
     6601 aaagttggca acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag
     6661 aatacccatt cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg
     6721 atcgggcttt tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa
     6781 aaagtatgtg gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg
     6841 gctacttaaa gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg
     6901 aaggatcgat tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac
     6961 tactttgtga tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct
     7021 atcatccgaa gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc
     7081 accagacaga agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg
     7141 ctcaccatag aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag
     7201 taatggatct ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg
     7261 ggaaattggt caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac
     7321 atgctgattt ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc
     7381 aatggtataa taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg
     7441 taagaaagag ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa
     7501 tgaatgaaac tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt
     7561 tgtttctagg aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca
     7621 atagcagaaa aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt
     7681 tacgaataca tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt
     7741 ttttcttgaa tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct
     7801 tgagctgata gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga
     7861 aatattggag catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta
     7921 agaatttagc aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta
     7981 gaacttgcgg ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt
     8041 gactatggtc aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg
     8101 agatagccac ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc
     8161 agcgtacttc cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt
     8221 agctgactgt gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt
     8281 gctcaatatt ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc
     8341 tttctccagt tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat
     8401 aaggatgccc ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc
     8461 acatcagtac gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt
     8521 gataccagtt aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt
     8581 ctttagaagc aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct
     8641 tattgtagca gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt
     8701 aaatatgaaa aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca
     8761 atactttata ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa
     8821 tgataaaacg caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa
     8881 aataaccata catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag
     8941 caatactaag tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag
     9001 atagatagtc tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga
     9061 tatgaaacaa aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt
     9121 gaacgtagat agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg
     9181 ctttttttat ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag
     9241 ccccgtggca gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa
     9301 acttcatatc ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg
     9361 gttgatgggc tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga
     9421 aacaaaagca gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga
     9481 tgtcaagaaa ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga
     9541 tgattaccat aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat
     9601 ttacttaggt gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac
     9661 acaacttgca gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag
     9721 tggacacgaa gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc
     9781 agaccgtcta ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa
     9841 aaacgaaatg atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac
     9901 aacttttact tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat
     9961 cgatgcatta tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt
    10021 cgaaaaatac tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac
    10081 gatctattgg ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg
    10141 gcatggctgg ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta
    10201 tcctgaaatg cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc
    10261 tgttccgttt gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta
    10321 tgatctggca aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata
    10381 ttgtgatgga ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc
    10441 tacgacggct gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa
    10501 tacagcattg gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac
    10561 agaaccatgg gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat
    10621 ccatttagct gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac
    10681 gccagagaag atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt
    10741 acactttggc gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc
    10801 acgtttagat atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca
    10861 aacaaacgaa gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga
    10921 aaaacaaatc aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa
    10981 ctgtcaaaaa gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag
    11041 ccaagaccgt caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat
    11101 cgggaaaaaa gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag
    11161 tcaaggaatg atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc
    11221 aaaatccatg ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt
    11281 tgtgtcatac cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta
    11341 cttgaagcca atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag
    11401 aagtacgagc ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac
    11461 agaaaaaaag cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt
    11521 ttttattgtc cttacattca taaagtttga atgcaatgac tttattacat ctatatttat
    11581 atctaacagt agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt
    11641 gtgctcttat atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg
    11701 atgattgttt tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt
    11761 tttgtcttca aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca
    11821 taccagaaaa tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt
    11881 aacaagatca accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg
    11941 acttcactaa gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc
    12001 atccgccagt tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa
    12061 ataccgatag gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc
    12121 acaggacctt tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt
    12181 gataacgttg caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac
    12241 tgctgttgtt ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga
    12301 taagaacgct taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac
    12361 gaaaaataaa aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa
    12421 aaagaatggt aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa
    12481 gaaagaggat gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag
    12541 ctaccgttat gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg
    12601 ctagtactca gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga
    12661 tctgtgctgg attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg
    12721 taaaatatat tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa
    12781 gcttgattta ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga
    12841 cgaacttatt acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt
    12901 ctgtgacagg gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt
    12961 tgattgttaa attaatacca attgctgtga ttgtcatatg gggattattg accccaggac
    13021 aaggaactgt ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat
    13081 tgagcagtgc gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa
    13141 tggcagggga aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga
    13201 gttttgtaac agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg
    13261 atcatcttgc cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa
    13321 tcggtggaaa gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt
    13381 atacactaac tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca
    13441 gtaaacagct gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc
    13501 agttagcagt agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt
    13561 tgatttttgt gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa
    13621 aaaaagcgcc ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta
    13681 ttgcgattct cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag
    13741 cattgatcgg tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa
    13801 aacgctcaga agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct
    13861 agatcttcgt gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact
    13921 tctgcaagta gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt
    13981 gattctcata cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag
    14041 cgagcacaag aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt
    14101 gaaaagtctt tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat
    14161 ccgacagagg ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact
    14221 ttaccaaagg ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg
    14281 aaagaagtcc aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta
    14341 ccaatcagta ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa
    14401 gatgttcgag atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa
    14461 cgatttcttg acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca
    14521 ttagacgtcc aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat
    14581 gccccttatc tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt
    14641 tatacagtag aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa
    14701 acatggacaa atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca
    14761 agagattata gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc
    14821 agatacgata gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca
    14881 tgcccaagag actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat
    14941 aagaaaaatc attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca
    15001 agctaaacca aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt
    15061 agaagcattg aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac
    15121 tagacaagac cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga
    15181 aaaactaggt gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt
    15241 gaagatgttt cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca
    15301 acatgagtga atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac
    15361 atggtttttc atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa
    15421 ggaaaatcgt agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag
    15481 gaatcggtgc attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga
    15541 tcgatgatcg tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta
    15601 ttgtacatca agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg
    15661 aggagcttcc gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga
    15721 tgcattttct tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag
    15781 tagctgatcg tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg
    15841 ttcaatatta tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc
    15901 agccaaatgt agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag
    15961 tgacagacga aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa
    16021 cgctttggaa taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag
    16081 caaaaagctt ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac
    16141 aagaattcgc cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt
    16201 gacagaagtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag
    16261 ttcctcatgt ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16321 ttaagaaaga ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa
    16381 acagcttctc atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact
    16441 attatgccga acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg
    16501 atcaaaaaat tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat
    16561 gtgtcttgac agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat
    16621 attaggagag agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat
    16681 tgattgggat gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt
    16741 cctttacttt ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg
    16801 cagtggctgt tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg
    16861 cattgcgagc attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc
    16921 atccgagtat cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt
    16981 tcaattttgt gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt
    17041 actttattgg taatatgcct gaaacatatt attctcaagg atatttttat acagtattca
    17101 tcttgatggg gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta
    17161 tagcaggaac actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa
    17221 atcggaaaga gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca
    17281 aaaaaaaaac tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc
    17341 taaaaagagc tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt
    17401 ttgatgactt gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg
    17461 tcgactattt cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta
    17521 gaaccagttt ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct
    17581 gatttcacta cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct
    17641 ggacgaatac cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt
    17701 tttccttcag gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat
    17761 gtcacattaa agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg
    17821 ggtgtatcat aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata
    17881 acgattcggt cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt
    17941 gtttcaagac gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca
    18001 tctaaatttg ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt
    18061 cccaatgcca cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat
    18121 tctgtcagac ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca
    18181 tatttacgta atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat
    18241 gcgtagtttt ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact
    18301 tttccgccga tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa
    18361 gtagttgact ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt
    18421 gatagattaa aatcagttac agaataattt tctgcattgt catatttttt atatacgttt
    18481 ttcagagcca tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt
    18541 taaagtataa atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt
    18601 ttttttgtca atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat
    18661 acactggagt aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct
    18721 ttgatcgctc atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca
    18781 atattggaaa aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca
    18841 accggcttgc cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga
    18901 gccatgattt cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca
    18961 cagccccatg aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg
    19021 ttagctacga atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta
    19081 gattttcgaa gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc
    19141 gtatggaaaa ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga
    19201 taatatgcga tataatacga acgtattgga taatataaac aataatatta gtttttcgta
    19261 tttgcagtaa atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac
    19321 agagtagaaa ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga
    19381 cgtaatgttc gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa
    19441 ggagatgcta aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt
    19501 attgattgca ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa
    19561 aatcagtgtc acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc
    19621 agggataggc gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt
    19681 ctttattgga tttacattga atgcatttat tgaaggagca atctacggat tctttttcta
    19741 tcgaaaagag atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat
    19801 caacttattt ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg
    19861 ggtcgtttgg gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc
    19921 catttattac gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca
    19981 cgctaaataa aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg
    20041 tgaattgtgc ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg
    20101 tgcccatttc gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg
    20161 tggacaggcg atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt
    20221 gttggatttt tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca
    20281 tagggggatt actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata
    20341 tctttaaccc ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt
    20401 catacatata agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc
    20461 caaaacgaag attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc
    20521 caaaactttt acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa
    20581 ttgttccttt tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg
    20641 cccctgtttt tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt
    20701 cgatcaaacg ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt
    20761 tgatattttt gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat
    20821 cgatcaaatc aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc
    20881 cagaaagttc gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca
    20941 caccaacagt gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg
    21001 ataaataagc aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt
    21061 gtgcccgttg tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag
    21121 cttcttcttg ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc
    21181 ccgagatatc cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa
    21241 cgatccaact cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg
    21301 gacttagacg catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt
    21361 gattgaaggc cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga
    21421 cgatatttcc gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa
    21481 atgcatcttt tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga
    21541 tgagccattg actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga
    21601 acagtcgatt gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt
    21661 gtaacgtcat cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa
    21721 tgtcttttgc ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt
    21781 tttttacttt cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga
    21841 ttgagcaagt aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa
    21901 gatatgatgt gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc
    21961 aagataagtc tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa
    22021 agagtcatct aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg
    22081 ctgtttttgt ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt
    22141 tggaagctga cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt
    22201 ttctgtggcg tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac
    22261 agaagtttgg ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac
    22321 atccattccg tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg
    22381 tgtcaaagtc gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt
    22441 cagctgaaat gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac
    22501 atggtcaaaa gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc
    22561 aagaatcgaa ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg
    22621 tgtgaagatc acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata
    22681 attgatcaaa gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc
    22741 aactgtcagc aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag
    22801 ggcagagata ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa
    22861 cttctctgtt tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt
    22921 ctgactaacg tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac
    22981 ggtttttttg atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa
    23041 cagacctact tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg
    23101 tgctctgatc aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt
    23161 tgtcattcgg gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt
    23221 attgattttt ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga
    23281 agcatagtat tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca
    23341 taagcccatt tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga
    23401 agccatcatt aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc
    23461 caaaatgatt tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct
    23521 cctattattt atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa
    23581 tttagaatga aaagagaaca agccttttca taaatgaaag ttttattatg aagaagcttt
    23641 agaaattgaa tagactattt tttttaaaaa aaagaaaatg agaaaacatg ccagtctctt
    23701 cattcatag
//
LOCUS       DAXLPI010000034        23636 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXLPI010000034 DAXLPI010000000
VERSION     DAXLPI010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.034"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(513..2009)
                     /gene="lysS"
                     /locus_tag="ACF3T5_001171"
     CDS             complement(513..2009)
                     /gene="lysS"
                     /locus_tag="ACF3T5_001171"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFD3631794.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2120..3124)
                     /gene="dusB"
                     /locus_tag="ACF3T5_001172"
     CDS             complement(2120..3124)
                     /gene="dusB"
                     /locus_tag="ACF3T5_001172"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFD3631795.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3125..4012)
                     /gene="hslO"
                     /locus_tag="ACF3T5_001173"
     CDS             complement(3125..4012)
                     /gene="hslO"
                     /locus_tag="ACF3T5_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFD3631796.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4229..6340)
                     /gene="ftsH"
                     /locus_tag="ACF3T5_001174"
     CDS             complement(4229..6340)
                     /gene="ftsH"
                     /locus_tag="ACF3T5_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFD3631797.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6430..6975)
                     /gene="hpt"
                     /locus_tag="ACF3T5_001175"
     CDS             complement(6430..6975)
                     /gene="hpt"
                     /locus_tag="ACF3T5_001175"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFD3631798.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6975..8420)
                     /gene="tilS"
                     /locus_tag="ACF3T5_001176"
     CDS             complement(6975..8420)
                     /gene="tilS"
                     /locus_tag="ACF3T5_001176"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFD3631799.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8540..9010)
                     /locus_tag="ACF3T5_001177"
     CDS             complement(8540..9010)
                     /locus_tag="ACF3T5_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFD3631800.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9056..9481)
                     /locus_tag="ACF3T5_001178"
     CDS             complement(9056..9481)
                     /locus_tag="ACF3T5_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFD3631801.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9548..9817)
                     /locus_tag="ACF3T5_001179"
     CDS             complement(9548..9817)
                     /locus_tag="ACF3T5_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFD3631802.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9828..11423)
                     /locus_tag="ACF3T5_001180"
     CDS             complement(9828..11423)
                     /locus_tag="ACF3T5_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFD3631803.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11438..14959)
                     /gene="mfd"
                     /locus_tag="ACF3T5_001181"
     CDS             complement(11438..14959)
                     /gene="mfd"
                     /locus_tag="ACF3T5_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFD3631804.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVSDQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14976..15536)
                     /gene="pth"
                     /locus_tag="ACF3T5_001182"
     CDS             complement(14976..15536)
                     /gene="pth"
                     /locus_tag="ACF3T5_001182"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFD3631805.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15889..16863
                     /locus_tag="ACF3T5_001183"
     CDS             15889..16863
                     /locus_tag="ACF3T5_001183"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFD3631806.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17033..18433
                     /locus_tag="ACF3T5_001184"
     CDS             17033..18433
                     /locus_tag="ACF3T5_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFD3631807.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18511..19233)
                     /locus_tag="ACF3T5_001185"
     CDS             complement(18511..19233)
                     /locus_tag="ACF3T5_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631808.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19312..19818)
                     /locus_tag="ACF3T5_001186"
     CDS             complement(19312..19818)
                     /locus_tag="ACF3T5_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFD3631809.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19941..21740
                     /locus_tag="ACF3T5_001187"
     CDS             19941..21740
                     /locus_tag="ACF3T5_001187"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFD3631810.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21808..23382)
                     /locus_tag="ACF3T5_001188"
     CDS             complement(21808..23382)
                     /locus_tag="ACF3T5_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFD3631811.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
BASE COUNT         6473 a         5102 c         4292 g         7769 t
ORIGIN      
        1 tttttggctg ccgtagcaac ttttatatca tatcagagag acaagcgatt gtcaactctt
       61 ttttaaaagt ttttttcgat ttcgtttttc gaaatctttc ggcaactcat ttaccataac
      121 ttatcgtttg ttatttgtca agaacttttt tgaagttttt ttcgatagaa tcaagcttcg
      181 caagtggttc ttttttaacg actcagttat cttatcatgt cgttagtaac atgtcaataa
      241 cttttttgtt atgtttttga ataaatattt tcttctgtct tttggacaag taataatata
      301 ccaactaaat ttagcttggt caacgaattt tttgtatttt ttcaagtttt tttcaataaa
      361 cgattattca taacttttct ttctttatcg ttcgttttcg tcttctttaa tgtctagaat
      421 agtcggcatt agcttttgaa ctttcgttat tatttattcc tttctacaca gcaaaagctg
      481 cctaaggaat tccttagaca gcttttattc gattatctca ttgttgggaa tagcaaaaca
      541 tcccgaattg attgtgcatc agttagcaac attactaatc tgtcgatccc gatgcctaga
      601 ccaccagttg gcggcatacc gtattctaag gcttccaaga agtcttcgtc tacaccgtga
      661 gcttcgtcat ttcctagttc acgttctttt tcttgcgctt cgaatctttc tctttgatca
      721 attggatcgt tcagttctgt aaaggcatta gcaaattcgc gccccactat gaatagttcg
      781 aatcgatctg tgaatcgagg atcttctgga tttttcttcg ctaaaggcga aacttcgact
      841 ggatgtccgt atacaaacgt cggttgtgtc aatgtttctt caacaaatgt ttcaaagaat
      901 tcattgataa catgtccgac acccatagct tcggttactt ctacattgtg tttttcggct
      961 aaagctaatg cttcttccaa tgtcatttct tgccagaagt ctacaccagt ttgttctttg
     1021 attgcatcta ccatatggat gcgtttaaag tcactttcta agtcgacagc ttgtccatca
     1081 tatgtgattt ttgctgttcc tagtactttt tcagctgcgt tacggataat accttctgtc
     1141 agattcatta catcattgaa atcagtatat gctgtgtatg cttctaacat cgtaaattct
     1201 ggattatgtg tagtatcaat tccttcatta cggaaaacgc ggccgatttc ataaactttt
     1261 tccattccgc caacaattag tcgttttaaa tgaagttcta gcgcaatacg caaatataaa
     1321 tcaatgtcca gtgcattatg atgcgtgata aatgggcgtg cggcagctcc acctgcctca
     1381 ttgtgcaata ctggtgtttc tacttcaata tagccattgc catctaaata gcgacggatt
     1441 tcgctgataa tttggcttcg tttcataaat cggtcaaagc tttcgcgatt actgattaaa
     1501 tccaagtagc gttgtctgta gcgttgttca atattcgtta agccatggta tttatctggc
     1561 agtggacgaa gtgcttttga caaaatcgtg atttctgaag ctttgataga aacttcgcct
     1621 gtgtctgttt tcatgatttg tccagttaca ccaaagaaat ctccaaggtc tgcatgtttg
     1681 aatacttcat aggcctcatc acctacttgg tcttttctta cataaatttg aatttggcct
     1741 tcacgatctt gtagatgcgc aaatccagct ttcccttttc ctcggcgagt catcatacga
     1801 ccagctacac tagcggtcaa tcccatttct gctagttctt cttttgttct tggatcaaat
     1861 agttcatgca attcttctga gttgtgcgtg cgttcaaatc gttttccaaa tggatcgatt
     1921 ccatcttcac gcaattgttc cattttttga cgacgtacaa gcatttgatc atttaaatct
     1981 tctacttgat gttgttgttc ctgtgtcaca aaagttcctc cattctcatg ttaatctctt
     2041 tcctataatg ccaatgaaac acaaaaaaag caagccgaac ttggaatttc ggcttgaaaa
     2101 ttcacgaaaa acttagaaat cacaacgttt gtttcaatag tactttttcc gctttttcta
     2161 caaattgatc cagcaggtca ttgatttctt gctgtttttc tgtctgattg attgctactt
     2221 tcactttcgc tgctcgagga atccctttta aataataagc cgcatgtgtt ctgaattctc
     2281 tacaagctac tttttctcct ttgagatctg ctaagcgttg taaatgaagt ttagctgtag
     2341 caattttttc ggctggtgtt ggttcaggaa tcagttcccc tgtttcgaga taatgctgtg
     2401 tgcggtgaat catccatgga ttgcccagcg ctgcacgacc aatcatcaca ccgtctgcac
     2461 cgacatagtc aagcatccat tttgcatctt ctggcgtttt gacatccccg ttccccataa
     2521 atggaatcgt caaatgtttt ttcacttctt tcaatacttc ccagtccgct tttccttcat
     2581 acatctgaac ccgtgtacgt ccatgcatag cgatggcact tgctccagct gattgtgctg
     2641 ctaatgcatt ttctacagca aagacgtgct cttcatccca accaatacgc atcttgattg
     2701 ttactgggat atttactgct gaagaaacag cttggaccat ttcgtatact ttctcaggat
     2761 ctaaaagcca ttttgctcct gcttctgctt tgattacttt atttactgga cagcccatat
     2821 tgatatctat tatatcggca gctgtgtttt cttcgacaaa ttgagcagct tcaactagac
     2881 tatctttatt tccgccaaat atctgtaaac ttaatggatg ttctgtttct tcaatgtgga
     2941 gcatctctaa agtttttttg tttctgaaat gaataccttg gtcactgatc atttcacata
     3001 cgactaagcc cgcaccaaat tctttgacag tgactcgaaa tgctgaattt gttattccag
     3061 ccatcggtgc aacaacgaca cgattaggga tttccacatt gccaattttc catgtgtgtt
     3121 ccacttaaat ccctcctaag gcttctttct tcaatccatc taattcttct tcgctatact
     3181 tgtatttgtt attacagaaa gaacaaaccg cttctgcacc gtgatcttcg tctatcattg
     3241 cttggatttc ttctgcacct aacgcgataa ttgcttctgc aaatttttct tttgaacagt
     3301 cacatttgaa ttgaacaggc attttttcca aaatgttcaa ttcttctgta ccaagcaatc
     3361 ctttcaatat ttcttctggt gtctgtcctt catctaagag ggtagagatt cttggtgtcg
     3421 tttttaattg ttcttcgatc ttgctgattg tttcttcatc ggcacctggc atgatttgga
     3481 tcatgaatcc tcccgccgtc ttgatgctgt cgtcagtatt gaccaataca ctcagtccaa
     3541 cagctgaagg gatttgttct gagatagcaa gatagtaagt aaaatcttca gcgatttctc
     3601 cagaaacaat cggcgtttgc ccagaaaaag gttcttttaa tcctaagtct tttgtgacag
     3661 taaacattcc ttgtgttcca actgctcccc gcacatcgat tttaccttca ctatttagag
     3721 gaagactgag atgcggattt ttgatgtaac ctttcacatc tccctttcca tttgcatcta
     3781 ctacaattcc tgttgctgga ccatcgcctt ggattcgaac agttagtttg tcttctcctt
     3841 tgagcgtcgc accaagtagc agactaccga ccaatgtccg tcccaaagca gctgaggagg
     3901 tgtgccaagt atcatgacgt ctttgtgcct ccgctacggt atttgttgct tgaacggcgt
     3961 atgctcggac gaatccgtcg tgagcaagtg ccttaaccaa ataatctttc atttgttatg
     4021 tactcctttt tccgttttta aactcatagt gaatcatact agcaactact gcttttttca
     4081 actttggaat gcttctttta aaaataattc agaatcatct agaaaacagg acatcaccct
     4141 atttacaact taaataaaga aatctcccga aaaccggttt ttgttccggt tttcgggagt
     4201 catatcagat caattgacct atattttttt atttgtattg atcgtctgaa tgattatctt
     4261 ctgattgttg agaatctgaa tcatgttctg ctttattttc ctcattcagt tcatgttttg
     4321 cttcttcaaa atcacgtttt tcttccgctt gtttttcagc gtctttttct tccaaagcac
     4381 gttttgcttc ttcaaaagtt ttcgcttctt tttcgcttgg ataatcattt gactcagtac
     4441 cttcaggcat cacaccgtgt tcaaacaatg acttgattgc tttggcatcc aatgtttcga
     4501 attccaaaag tttttcagca atcagtttat gctgtgcacg atgagcttcg atgatttctc
     4561 gagctttatc atgagcttcc atcaagattt ttcgtacttc ttgatcaatc tcgaaagcga
     4621 cttgttccga gtaagctttt gtttgaccat agtcgcggcc aacaaacact tgatggtttc
     4681 cttcatattg aacaggacct aaacgatcac tcattccata ttctgttacc atgcttcgtg
     4741 ctagagctgt cgcttgctca aagtcattgg aagcaccagt tgattggaca ttgaagatga
     4801 tttcttcagc tgtacgtcca ccaagtaagc ctacgatttg ttcaaacata tcttctttcg
     4861 tcatcaagaa ctgatcttct tttggtaaag cgatcatata tccgccggcg cgtccgcgag
     4921 ggatgatcgt tactttgtgg acgacccgag cgcgactcaa tactaatccg acaatcgtat
     4981 gaccagcttc gtgataagca accatttcgc gttctttttt gctgattacg cgatcttttt
     5041 tagctggacc tgcaatcacg cgatcttccg cttcatcgat atcagaagca tcgattttct
     5101 ttttatttct tctagcagct actaacgctg cttcattcaa gacattttct aaatctgctc
     5161 cggcaaaacc tggtgtctgt tgagccacaa ctttcaagtc aacgtcatct gccatcggtt
     5221 tattccgtgc atggactcgc aagattgctt cacgaccttt tacatctgga cgtccaacta
     5281 aaatctggcg gtcaaaacgg cctggacgta gtaacgctgg gtctagtaca tcagaacggt
     5341 tggttgcagc aatgacgatt actccttcat ttccgtcgaa gccatccatt tctacaagca
     5401 attggttcaa tgtttgttcg cgttcatcat gtccgccgcc cataccggca ccacgttgac
     5461 gacctaccgc atcaatttca tcaatgaaaa tgatagctgg tgcgtttttc ttcgctgttt
     5521 caaacaaatc acgaacacga cttgctccaa caccgacaaa catttctacg aaatcagaac
     5581 ccgagatcga gtaaaacggt actccagctt ctccggcaac agcttttgct aataaggttt
     5641 tacctgttcc tggaggtcct tctaaaagta cacctgccgg gatacgggca cctaattcaa
     5701 caaatcggcg aggatctttc agaaattcaa caacttcaac tagttcttgt ttttcttctt
     5761 ccgcacccgc tacatcagaa aaacgtacgc gattagcttt tttgtctgct tctttggctt
     5821 ttgatttacc aaagttcatg acacggccgc caccgccacc gccgccgcct tgttgtccca
     5881 tcatcatgta aaagaaaaag atgatgatta cgattggcaa gaagctaagc aatagggaaa
     5941 cccatatacc gctgcttgat tcttctttta ctgttgtagc aactttgttt tcttttgcca
     6001 gttctgttac ttggctcaat gttgtatcat ttggcaaaat gattgttgtg aaatgggtag
     6061 tagtcgtatc tgttgaacct aagatagcta acccaccgct gttagcgacg gtctgctgtt
     6121 ctttgtattc accagtgatc ttatatacac catttgcagg ctgcacttca aattctttga
     6181 tcttgcctgc ttgtagttgt tctgtgaatt ttgagtactc gatatctgac gatcgctggc
     6241 cgttattacc aaaaatgaaa taaacgacca ttaccatcgc taagattacg aggacataat
     6301 acaaggcatt tttcatcatg ccgttgttct ttttgttcat agttgccctc cctaagcgtc
     6361 aatttaattt aacagttttt tatcaatcct gaccattcta ttgtaccata atttaattga
     6421 gatacagcat taatttgatt gataaacact aggtttcaat acaccgatgt acggaaggtt
     6481 acgatatgct tctgcataat ccaagccata accgaccaca aattcatttg gtacgttgaa
     6541 gccaacataa tctgcttcga tatttactac tcttccttca ggtttgtcaa gtagtgtaac
     6601 aattttgact gattttgctt tgcggtattt gaaaagatcc actaaataag caagtgttct
     6661 tccgctgtca atgatatctt caacgatcaa cagatcgcga ccttctacat tcgtatctaa
     6721 gtctttgacg atttttactt cccctgacga gactgttgca ttcccgtagc ttgaaacatc
     6781 catgaaatcc aattccaaat acgtatcgat agaacgaacg atatctgcca tgaatggtac
     6841 cgctcctttt aagacaccta ctactagggg attggtgttt tggtaaattt ctgttaactc
     6901 tttgcctagt tctttgcaac gaacttgtat ctcttcttgt gagatcaaaa ttctctcaat
     6961 atccttttcc aacatcaggt tctccttcca attgcttctt tttgatattt gtaaagaagt
     7021 atgtagtgta ttttatcagt ttcttcacga atactcaaat atgaaaaggc aaaaggtatc
     7081 aagctatata tatttctttt ggcatctgta atgatccaag catgctgcct ttgtgaatta
     7141 ggtactttct gatcgataaa atatctagat actttttttg tgagattctc tgttaattta
     7201 atgcggtctc ctgcttttct tttttcgaca tacagttgca catcagcagg aatccaaatg
     7261 tcatgagaaa atagagacca tgaatctgtt gctttgatgt cccctgcatt ttcaggtttg
     7321 accagaccaa tccattctgt ttcattcaaa tatattcctt gttctggaat cagcaacaag
     7381 cgctggtctt ctgtctggct agttgcttgt ttttcttttt tagagagtac aacaaaatga
     7441 tactctcttt tcagtatcca attattgcca atactgagtt gccattggct ttttttctgt
     7501 tgccactggt ctaaaatcga ctccatttgt tgttctttca aaataattcc tgtttttgag
     7561 aatgctttgt cgaaaaagga gtaaaaaaaa tagtatcgtt cagcatctgt catttcttca
     7621 atcatttctg cagaaaatcg aaattgttcc tcccactgtt cgacttcctt ttcgatcaac
     7681 tgtcccatat atttttgaat gacttggtct gcccattgga tctgttgcga aaattgctgg
     7741 aaatgtctca acgcctgtgt attttcttgt ttcaattgag gaactaccaa atgacgaagc
     7801 cgatttctct ggatatctaa caattggttt gtctgatcct caaagtaaac aagctgcgat
     7861 tccgctgcat aagtatatag gtcttctttg ctaaaactca gcaacggacg aatcagtctg
     7921 cccgtagcaa atggttgtac ttctttgatt ccagaaaaag tatttaactg tcccccacga
     7981 acgattttca ttaacaccgt ctcgatttga tcatctccgt gatgagcagt catcaacgtg
     8041 tcatattgtc cttcttccat cacgcgagtg aattcttgat aacgaaagtt acgagcttct
     8101 gtttctatgg cggatttggc tggatcttcc caaatactta catataaagg gagattcttc
     8161 tcttcacaat atgagcgaag ataggcagct tcttctgctg attcttctct taattggtga
     8221 ttgatgtgga cgatacctaa agaaaaaccg atttggcgtt gcacttgtaa aagtaaatgg
     8281 agcaaaacca tcgaatcaac accacctgat accgctgcta aaattctcga tccttgttgc
     8341 cagaatcctt gttcattccc gtgccgaata aatgactgct gtaatagcgg tgcttttttc
     8401 ccgccttttt caacgttcat cctttccaca ccctttcttc ctaaaaaaaa gagagcggga
     8461 aaacgtctaa aacgacaatt aacctgccct ctgttgatac tatgacccaa atagtcgcac
     8521 tatcattatt tgcggctttt tagctgcgac gtccgccacg gccaccgcgc tttccttctg
     8581 tgtttcgctt caatgaagat aagcggtcat cgctatcttt caaaaatgaa ctcatcaatg
     8641 aatcgaagtc ttctttttga gctgaaggtt gactgcgaga aaaagatttt ttctgtgctg
     8701 gacgatttcg gttgtcacgg ggatcttttt gttgatattt tttaggacgg ctttcttctc
     8761 cagattcagc aggttgttcc tgcgctttcc ggatagatag tccgattttt ccatcatcat
     8821 ttactgaaat aactttaact gttacttcat ctcctacact taaaacatca tggatgtctt
     8881 ttacatagcc atttgacact tcgctaatat gaactagacc cgtttttcca gcacctagat
     8941 caataaacgc accaaaattt gtgattcctg atacttttcc ttgcagtttt gctcctacct
     9001 cgattgacat aaaaaaaatg ttcctcctaa ttatttccct tttttatatt cctgcttatt
     9061 ttgcagttgt agaactagtt gattgatctg tttcatttgt cgagctgtct gtatttgatt
     9121 ctaatatagg ataaactttt tcaccgtctt ttgagtaaaa gaatttgctt ctggcaagtt
     9181 tagctacata ctcatcatct ttcagcaatg aaacatcttg gtgcagctga tcaacgtctg
     9241 cgttgacttc tttcatttca gtatttgctt cgactctcaa ttcgttcaat tgatgcatcc
     9301 tttgcatgtc atggaaaatc tgcacaccta ttatcgcgaa aatgaccaaa gcaccaacaa
     9361 aaattgcagc cagtctgcgt cttcgaaaaa tcagttgacg ttgttgttgc tgaaattcag
     9421 catacttttt ctttgcatat tccgtatcta gcgcagcgat tttattgctt tttttcgtca
     9481 cttggattcg ctccaatctt tttgattgtc tttttcatta taccaatatc gtgaaagctt
     9541 gtctaatcta tttctctagt gtttctgtgc gattttcaga aataatttcg tacatttttt
     9601 gtgcatcttc ttttttggtt gattcatgta attccgtaat tttcacttcc aacgttttgt
     9661 tgccaaactg gactttcaaa atatcgccta ctttgacatc actggaagat ttcgccaatt
     9721 taccgttgat ttgaattctt cctttatcag ctacttcttt tgctactgat cgtcttttga
     9781 tgattcggga aatttttaaa aatttatcta atcgcatatc ttattcctca ctttttaatt
     9841 tttttattta atcgcaggat tttcttacca aaaggcaaca ttagccattc tcgtactgta
     9901 aacagacgaa gacgaatcac tgcataaatg aacacagaag ccccgatccc aactcctatc
     9961 acgctgacaa aaaaagcagt ggaacgatga gttacaggac caaatactat attcaataca
    10021 ccataataca gcagcagtac tactgtcata caaagaaggc tgaacatcaa cttccgtcca
    10081 aaatgccgtt ctttccaaaa agcatttagc tcttttgttt caattcgaac aaaataccaa
    10141 agcgtcacac ctaatcctaa aagggtagac aggcttgcac ctgctgttcc taataaaccg
    10201 gtcagaaaag aagtagtcaa tccttttacc aatagtcccc atcctgcgcc ttttaaagaa
    10261 gggcgaaacg agttcttgct ttgagcaata ctttgataag cttggatgac tgcagtgaaa
    10321 gcaatggaaa aaacaaataa tacaagcgct gcatttcccg cataatcttt aaacaacgca
    10381 taattgatat aagggagcag caaggctaat cccaaagacg cagctaatgc taaagcggtc
    10441 gtcaatctaa gatagatttt ggctgtctgt aaaaactgac gatagatccg gttggtcaaa
    10501 tgacgagtca acgcaggcag gaatgtcgca cttaatgcag tggcaatcac taaacctaat
    10561 tgtactaaag gctgacctcg atcatagacc ccttttgcta tcttcgcacc gtattctgac
    10621 agtccgtaaa cttccaaagc attttttaca aagaaagagt cgatcagctg gaaaaagata
    10681 agcaatccac tataaatcga aaccagtccg ccttctacca gaaaacgccg gatcaagggc
    10741 tttgacggct gtttgaacaa tggaaaccgt cggaagctta acgctcctcc atgaatcttt
    10801 tgttcatagt agtgaaggat cccatatgcg cataaaccgc caaaaacagc tccactcatt
    10861 gctgccgtcc ctgtttggta caccgtccat ccaagctttc taaaagcaaa cgcactacag
    10921 acaatcacaa aaacacgaac gaactgctca actacttgag aaacagcagt tggttccatc
    10981 aagaatcttc cttggaaatt ccctcgataa aaagatagac caggcatcag caaaaaggta
    11041 aaggaaacta cttggatcag cggtttcaat tgtatgtctc ccatcatcca agcaatccag
    11101 ctgctaaatg caaagacaaa caaccacaaa aagacacctg tccaaaatac taagggataa
    11161 agattttgca aagcttgctt tcgctcgaaa ggattctttc tttctgcaac gtactttgat
    11221 atgaactgcg gaagaccgga taaagctaaa gtcattgcta tcccataaat tggataaact
    11281 tgctgataaa cataaaaacc ttcgtcgccg actaagtttt ggaggggaat tcggtaaagg
    11341 gcacttaata cttttgctat gaaagaagca atcgtcaata caaatgctcc ttgcatgacg
    11401 cgccgcattt ctttatgatc caaatcattg ctccttccta cgctgacaga tatttttgct
    11461 cacgcaacgc tttgacaaac ttttggattt cctgaagcca tactgcttct tccatttggt
    11521 tggggatcgt caagcgtatc accatctgtt ctttttcaac gcctagactg gcttttagtt
    11581 ttgtcgctgt cagtgcttcg aataactgtt ccacactata gcgttttgtt ccaactttac
    11641 ttagagtaaa gactatatgc gcctcctgtt tccttatcgt ttctaggagc gctcgatcgc
    11701 catccatttt gatctgccca attgtcaaga gatgtgctac ttcttctgga tattctccaa
    11761 aacggtcgag cagatcatct tctagttcat ctagcatttc ttgcgaatca agttcacgaa
    11821 ttcgtttata gatctcgatt ttctgccgtt gatctgacac gtatgtttca ggtagatacg
    11881 catcgacacc gaggtcgatc tcaacagtgg tcttctccac ttgactattc tttccttgtt
    11941 tacgagaaac agcttcgctt agcatttgag aatacatatc gaaaccgact gcatcaataa
    12001 atccatgctg ctgagcacct aagagattac ctgcaccgcg aatggaaaga tcccgcatcg
    12061 caatcttgaa tccagatccc agttctgtaa aatctttgat tgcctgaaga cgtttttcac
    12121 tgacttcgtt aaggatcttc tgctgctcat acataaagta agcatatgcg acacggctgc
    12181 ttcttcctac ccgccctcgc aattggtata acgtggataa tcccatataa tcagcgttct
    12241 caacgaacaa cgtatttgcg ttcgggatat ctactcctgt ttcaatgatg gttgtagtga
    12301 caagaacatc atattgacct tcaataaatt caaaaagcgt attttctaat tgcgcctctg
    12361 tcatttgccc atgagcataa ccgatccgtg cttccggaac gagttcttgg atttcttcta
    12421 ctttttgttc gatcgtttct acccgattat ataaatagaa tacttgtcca cctcgtccca
    12481 tttctcgatg gattgcttcg cgaatagccc caggattttt ttccataaca tacgtttgga
    12541 tcggataacg attcgcaggc ggtgtttcga ttactgaaag atcccgcacg cctaacatcg
    12601 acatatgcaa agttctcgga atcggtgttg cagtcagagt caaaacatcc acttgtgcac
    12661 gtaactgttt caatcgttcc ttatgtttta caccaaaacg ttgttcttcg tcaatgatca
    12721 gtagacccaa atcgctaaat tcaatatctt ttgacaaaat tcggtgggtt cctacaacaa
    12781 tatcaacttg acctgtacgc agttgttcga tggtttcttt ttgttgcttt cttgttctga
    12841 aacgacttaa tagcccgata tttacgggga aaccttcaaa tctttccagc atcgtttcat
    12901 agtgctgctg tgccaagatc gttgttggaa ctaaaaaggc tacttgtttg ctttccttta
    12961 tcgctttaaa ggccgctcga agcgctactt ctgttttccc ataaccaaca tcacctacga
    13021 gtagtcgatc cattggtttt tctttttcca tatcccgttt gatctccgct gcacttctca
    13081 actggtcatc agtttcagag taggggaagg cattttcgaa ttctttttgg taggcgtcat
    13141 caggtccaaa agcatatcct ttttctgact ctcttgcggc atataatttg attagatcgt
    13201 cagcgatatc ctcgattttt gaagagactt ttcgcttggt tttcgtccat tcacttccgc
    13261 ctagcttatt gatgcgaggc gctttggctt cagaggcgac atatttttgg atcagattca
    13321 gttgagtaac aggaataaac agtttgtcgt cgttttgata caaaatagtc atatagtctt
    13381 ggtgaacgcc atctacttct agcgtttcca tcccaatgta tttcccgata ccatgattgg
    13441 catggacaac gtaatctcct gcttttaact cattatagct tttcaatcgc tctgcgtttg
    13501 ttactgtttg acgtctcgtt tgttttttcg tcgttttttg gaaaatttct ttttctgtga
    13561 tagctactat tttttcttga ggaagttcaa aaccagcctg caaagctcct tcaactaatt
    13621 gtacctgtcc tctgatcaat gcattttttt ctgtttctac tactaatatg tcttcatctc
    13681 gaagcatttg ttctgctttg cgtattctgt cctttgtcgg aataaagatc acaacagtct
    13741 gtttttgctt gcgccaacgg tccatttctg ttttcagcaa tggcatttgt ccgaagaact
    13801 gttgcatcgt ccgatattga aaattatgga ttgcttggaa acgaagattt cccatccctt
    13861 tttggaagag tgagaaaaag gttgtggtga acttttcctt ctgcacgatt ttttgtatat
    13921 ctgctgaaaa tgtctgctca gaaaacaccc gcaattcgct gattttttga gtctgccatt
    13981 ctgccgcttc cctttcgatt tcccgatttg tttccatgat tcgctgataa tcatcaacga
    14041 ctagtaaact atttgaaggg aaataatcca agatcgtcac agattcttgg taaagaagat
    14101 ctgagtagaa ttttgcttct tctgtcggga cacctgcttg ccagcttgaa gtcagctgac
    14161 cgaagtaatc ttgcaaaaac tcgcggtctg cttcttctgt ggtcgcagca agccgttttt
    14221 ctagcatttt ttgcatttgc tctattccat gttctagatc ctctggtccg tagacttgtt
    14281 cactcgttgg ggaaacccag atttcttccg ttttttcgat tgagcgttgt gtttctgctt
    14341 caaaataccg catagaatct atctctacat caaatagttc tacacgaaca ggataggttg
    14401 tcgttaacgg gtaaatatcg ataatgcttc ctcgaatact gaattctccc ggttttgcga
    14461 ccatctcttc tcgtacatag cccatcaaag tcaattggcg aggaagattt tccacgtcta
    14521 tctcatcacc aatatgccaa ttcaattgcg cctgtagcca agttttctta ttcggcagtc
    14581 gtttgtgcaa agatgcgact ggcaacaaat agatacctgc ttttttttct tgaagtgcgt
    14641 taagcgttgc aactctctcg cctcgcgctt ctggagaaga aaaggccatt tcagcagaaa
    14701 gcacttcatc cactggaaat aaatggatct gatcttgcgg aatcacatgt tgcagatctt
    14761 ctgctaactg attcgcataa tacaaatttg gcatcagaat aatcatgttt ttatttgttt
    14821 tcttaaacgc tcgagcaaat agaagtgttt tcgctgagcc cgctaatcct gtgattagtt
    14881 gtctaggcat tttttcttct aaattgctga cccattcttt tacttgcgga tcttttccga
    14941 ctaattgaat aatatccatt ccttttcctc cttcctcaat tgaatcgatt catcgcatcc
    15001 acgaacgaat gcccttctat gagatattcg actgctttaa cagattgaca catgctttct
    15061 tctatcaaag gctgattttc tttgctgaaa ggactaagca catgctggac tactgttttt
    15121 ttcccttctg gcctgccgat tcccaccttg attcgatcaa agacattcgt attcaaatgg
    15181 gaaataatac ttttgatacc gttatgcccg ccggcacttc ccttttgacg gagccggatt
    15241 tttcccactg ccagatccaa atcatcgtag attacgacta attcctctgg ataaatccca
    15301 aaataagtca ttagtgggcc gactgcacga ccagattcat tcatgaatgt ttgtggtttg
    15361 actaaaagga tcttttctcc attatgaaaa aattcggcaa cttctgcttc gaacggattt
    15421 tttttgaaag tagcttgatg ctcgcgtgca attttatcca cgaccataaa gccgatattg
    15481 tgttttgtat attggtattt ggtacctggg tttcctaacc cgacgatcat tttcattttt
    15541 gttttccacc taattctaaa aagcagcaca aaaggctgga ctgctaatta gcggtccagt
    15601 ccatcgagct tttttctttt ttacacttgt caatgcatag tataacacaa aacaaggcgt
    15661 ttcggctaaa agagaatcgt taataaatga taaagaaatt aaaaatttct tcaaagttta
    15721 atgaaataat tgatatataa aaatagtgaa ataacgcaca tgtaagcttt tacatataat
    15781 acaaccttgt tatctgttat attttatagc caaatagcgt gacatggggc ttgagaaatg
    15841 ttagcataag catgggaaaa aacaaatcaa tggaaggatt gatactaaat gactgcagct
    15901 gcagaaaaaa aagatcatca aaaagttatt cttgttggag acggcgccgt aggttctagc
    15961 tatgcctttg ctctagttac tcaaaatatc gctcaagaag taggtattgt ggatatcaat
    16021 actgctaaaa ctgaaggaga cgcaattgac ttgtctcacg ctttggcatt tacttcacct
    16081 aaaaaaatct attctgctac ttacgcagac gcacatgatg ctgacttagt tgttattaca
    16141 gctggcgcac cacaaaaacc aggtgaaact cgtttagact tagtaaacaa aaacctaaaa
    16201 atcaaccgcg atgttgtcac acaaatcgtt gaatcaggat ttaacggtat ctttttagtt
    16261 gccgctaacc cagtagatat tttgacttac tcaacttgga aattctcagg tttccctaaa
    16321 gaacgcgtaa tcggttcagg tacctctctt gactcagctc gtttccgtca agcacttgcg
    16381 gaattagttg acgttgatgc acgtaacgtc catgcttaca tcttaggtga acacggagat
    16441 tcagaattcc cagtttggtc acatgcaaat gttgccggct tgcaaatcta tgaatgggta
    16501 aaaaacaacc cagaaatcga tgaagaagct atggttaacc tattctttgg cgtacgtgac
    16561 gctgcttaca caattatcga gaaaaaaggc gctactttct atggtatcgc agtagcatta
    16621 gcacgtatca ctcgtgccat cttagatgat gaaaatgctg ttcttccttt atcagtattc
    16681 atgaatggcg aatatggatt gaacgacatc tacattggtg cacctgctgt tatcaaccgt
    16741 caaggtatcc aaaaagtaat cgaaattcct ttaagtgaca gcgagcaaga ccgtatggct
    16801 gcttctgcta agcaattaaa agaaattcta gatgaagctt ttgctaaact agacgctgaa
    16861 taatctttga acatgacgga aaacatctat tcggtgtttt ccgttttttt attttatcac
    16921 ccttatggtt tctaaatttt ttcataaagt agagataacc ttcgtctttt cctctaaatt
    16981 atgctatcat gagaccgttc caccctaaac ttttacttaa ggagttgtcg atatgacaag
    17041 atcttcgcat cgaaaaaaat cgaaacatgt tgctttgctt tcagttcttg gtgttctctt
    17101 agtgctgatc agcttttata cctatcgcag tacatactac acaaagcatt ttttgccgaa
    17161 tacagaaata aatggaataa atgtcagcaa tctaacagtc gaaaaagcaa atgaaaaatt
    17221 aaaagaagct tattctgata aaaaactggc tattaaagaa gatggaaaag tctggcagga
    17281 agtaccaaaa tccgagctcg gctacaaaga tgatttcact tcggagcttt ctccgatcct
    17341 aaatgaacaa aacggatgga cttggggaat gacttatgtc tcagcggcag aaaaacaaga
    17401 aatagatcct gtcagtcaaa accaccaaaa actagacact accgttcaga cattgacaac
    17461 taagctgact gatttgaaca aagaccggac acctactact gatgcaacga tcgaaaaagc
    17521 aggtgactct ttcgttatca agcctgaagt caatggaaat acgatcgacg tcgatgctgc
    17581 ggtcaaacaa ttaaaaagtg cggtaaactc tggtaaagat acaattgaat tgactgaatt
    17641 caaagaaaaa ccaaaagtaa catctgaaga cagctcactg aaagaacaac tcgcatccat
    17701 gaatgctatc gcaaatgtaa aagctactta cagtataaac ggtgagactt tccagatccc
    17761 atcaagtgat attatgagtt ggctgaccta caatgatgga aaagtcgatt tagatacaga
    17821 acaagtacgt caatatgtaa cagatttagg cacaaagtat aacacaagta cgaatgacac
    17881 aaaatttaaa agtacaaaaa gaggagaagt tacggtccca gttggcactt acagttggac
    17941 gatccaaaca gacagtgaga cagaagcatt gaaaaaagcg attctggcag gccaagattt
    18001 tactcgttca cccattgtgc aaggtggcac aaccgctgat catccattga tcgaagatac
    18061 gtatatcgaa gttgatctgg agaaccagca tatgtggtat tacaaagacg gaaaagtggc
    18121 ccttgaaaca gatatcgttt ccggaaagcc aactacacct acacctgctg gagtcttcta
    18181 tgtttggaat aaagaagaag atgcaacttt aaaaggaaca aatgacgatg gaacacctta
    18241 cgagagtccg gtaaactact ggatgccgat tgactggaca ggtgttggta tccacgattc
    18301 agactggcaa cctgaatacg gtggagactt atggaaaacc cgcggttcac acggctgcat
    18361 caacacccca ccaagtgtaa tgaaggaatt attcgggatg gtcgaaaaag gaacgcctgt
    18421 attggtcttc taacaaaaat aatatataaa aagaaagcac ccgtacgatt ggtacaggtg
    18481 ctttcttttt attatctaac aatgtgctac ttacatttca gcagcagtaa agacttctga
    18541 aacatcatca tcgtcttcta gcttatctac tagacgttcc aattgctctt tttgttcctc
    18601 atttaaatca atcgtcgttt gtggaatcat cgtcaattct gcttgcgcca aagtgaactc
    18661 tttttccaac tcatctcgta cagcagcaaa atcttctggt gcagtataaa tctcgaatac
    18721 ttcaggagaa gtttgcagat cttctgcccc cgcctctaat acatcctcaa acatcgtctc
    18781 ttcatcaaca gtcaacccat cgcgttcaat agcgatatat cctttgcggt caaacaagta
    18841 actgactgaa cccgtttctc ccagtgatcc accgtttcga gtaaaagcta ctcggacatt
    18901 tgtcgctgtc cgattacggt tatctgtcaa tgcatggact aagattccta ctccacccgg
    18961 accatatcct tcatacgtga tttcatcgta attcgtttca tctacggcac tcgtagcctt
    19021 tttaatcgcc cgttccacat tgtcgttagg catatttgca gcttttgctt tgtccacaac
    19081 aagccgcaaa gctggattca tagatggatc aggaccgcct gctttagctg ccatataaat
    19141 ttcacgagaa agtttctgaa agactttccc tcgttttgca tcttgggcat tttttcttcc
    19201 ttggatatta tgccatttac tatgtcctga catgattttt cctcccttgt tcttttgaga
    19261 atacacttct attctagtac tatttcacaa aaaaacaaag ccacagccaa tctataaacg
    19321 cttcttgaat gccgcaaaaa gcacacataa tatcacagca gtcagagcac cgatcgaatc
    19381 caacatgaca tcttggaaca taggtgttcg tccaccggtc atcatctgat ggaattcgtc
    19441 caaacctgcg taccctgttg ctgaaagcca agcaaggatc gctgtcagcc accaaatttt
    19501 tagcttgggg aaaacaccta aaaacaagct gcctcccaaa ataaaatagg taccgaaatg
    19561 tgcgcccttc cttaaaaaga actctaaaaa tttggcgtag cccagatggt tgatactcac
    19621 catacttcca ccataattaa aacgtatacc cgctaatgtg tctttaaatg gctgattagg
    19681 cagccagctt tctaatcgcg aaacttgtga ttgctgtccg taagtttgag aagaactgta
    19741 aaataagatt ataacgacta tcagtgcgat tactaaataa atatttccgt ttttcatttg
    19801 tttttttgaa gaattcataa gtcacctctg tattttatct tagacttttt ttgaagaaac
    19861 aacaatcatt ttcatagaga cttcttccat aaaaaagtgc tatacttaat tttgtattac
    19921 aatatgaagg gagttttttc atgagttatt cattaaattg gaatctagat tctatttttt
    19981 ccggaggcag tcattctgat gctttgaacc aacggatgaa gcaattggaa gaccaaatga
    20041 acgagtatta tcaccgcgta acaaaatggt cacctagttc tgacaatgca gagcagttaa
    20101 acgctattct tcaattgcaa gaaacgatta cgaatggttt tacccaatgc agcagctata
    20161 ttactgcatt gctttctgcc aatgtcaatg attcagatgc aaaggtctta tcaggcaagc
    20221 tttacgcaat gctgcctcgt ctgcaatcag ccgaaactgt attgtcaaag aaatttgcag
    20281 aaatatctga caatgattgg aatcagatgc tttctcaatc ttcgtttgaa acaattgctt
    20341 ttcgtttaaa cgagattcgt cgtgatggct cacaattatt atcagaagct gaagaaaaca
    20401 ttataaatac gctttcttta gatggtctaa atgcttggag cagtcattat gatacgattg
    20461 tagccagcat ttcgattccc ttcgaacaag atggacaagt agtcgaatta tctgctggac
    20521 aagccttcaa taagatgatg ggcgatccag atccaaaagt cagagaaaca cttttcgcag
    20581 catgggaaga agcctggaaa gaaaaggcac ccctcttcac ggatactttg aatcatttag
    20641 atggttttag actttctgac tatgaacttc atggtgtcac agattttcta caaaaaccat
    20701 tagagtacaa tcggttgaag aaagaaacat tgtctgtcat gtgggataca atccagaaaa
    20761 acaaacaacc ccttgttaat tacttgacta gaaaagctaa tcttttcggt aaagaaaaaa
    20821 tggaatggca agatcaagat gcacctatta ttttaggcga tttgaaagaa aaaaccttta
    20881 gttttgacga agcagcagca tttattatcg aaaactttaa caaattcagt ccaaaaatgg
    20941 ctaaatttgc ccaatctgct tttgaaaaga gctggatcga agcagaagat cgtcctggga
    21001 aacgtcctgg tggttattgt actgagttac cagaaacaaa agaatcaagg atctttatga
    21061 cctacagtaa ttctgttaat gaggttgcga cacttgctca tgaactagga catgctttcc
    21121 atagcagcac gatgtgggat ttaccttctt taaatcgcga atatgccatg aatgtcgcag
    21181 aaacagcaag tacattcgca gaactgatcg tagccgatgc tacattaaaa gtcgcaaaaa
    21241 caaaagaaga aaaaatcaat ttgctagata caaaactgca aaatgcttta gctatgttca
    21301 tgaatatcca ttcccgattc atttttgaaa atcgattcta tgaagcacgg caagaaggat
    21361 tagtctcaga agataagatc acagagatga tggttgaggc gcaaaaagaa ggctatcaag
    21421 gagctttagc tacttaccat ccatatttct gggctgcaaa attgcatttc tttattgatg
    21481 acgtaccgtt ttataacttc ccatacacat ttggctacct ctttagtttg ggcatctatg
    21541 cttacgccaa ccaaaaggga gcaaattttg aagaagaata tatccattta ctacgcgata
    21601 ctgcctcaat gacgacggaa gatcttgcga agaaacattt gaatgtcgat cttacccaac
    21661 cagatttttg gcaagcaggt atcgacatgg tgactgaaga tatccaaacg ttcatggaat
    21721 tgacagaaga atttatctga tttctatttt atcacaataa aaaaagagtt agtccagaat
    21781 gggctaactc tttttgatta atcattctta catgctgaca gcaaagtcag gggcaaacca
    21841 ttgaactgaa ccaactttta cgctttcgcc tggttgagga gcatggatat attgtcctcc
    21901 gcctaaagca atcgctacgt ggtaagttcc gccttgtgac ccccagaata acaagtctcc
    21961 agcttttgct tgagaaactg aaatttttgt tccagctgat tcttgaggaa ctgtccaacc
    22021 accgatgtca cgaccagtta cttgcatgta aacatagcgt gtgaaccctg agcagtcaaa
    22081 gccacttgga tctttaccgc cccaaacata tggagtacca atatatttgt aagcttctgc
    22141 tacgattgct gcaccattca cacttccaga aggagctgga gcaggtgtta caggttttgg
    22201 ttgttcaggt gttggtgttg gcgttggagc cggagtacta cttcctgtgc cgtttcctgt
    22261 atcaacactt tgatctgttg atggtgttgt aggcgcaggt gttgttgatt cttctgttgt
    22321 tgaagattca ggcactgttg ttgattcttc tgttgctgaa gattcaggca ctgttgttga
    22381 ttcttctgtt gctgaagatt caggcgctgt tgttgattct tctgttgctg aagattcagg
    22441 cgctgttgat tcttctgttg aagattcagg tgttgttgat tcttctgttg ctgaagattg
    22501 tgtagctgat gatgattctt cagtcattga agattgtgta gctgatgatt cttctgttgc
    22561 tgagctttgt gcagtagatg aagcttgagt agcttgtgct tcagcctctg cttgttcgcg
    22621 tgcttctttt tcagcttttt cttgtgcagc ttgttgacga gcttgttctg ctagacgttg
    22681 ttgttcacga atacgagctt gctctgcttc agcttctgct ttttgacggt tcaagtcagc
    22741 tttcttgtct tcagcagttg cttgttcagc agctaatgaa gttttcagta cgttcaaatc
    22801 agcttgttta gataaaagat cgcctttttg agattccaaa gcagcttggt tttcagctaa
    22861 ttcttttagt ttagcatcgt tttcagcttt tttgtcttct acagcttttt tatcttgttt
    22921 ttgttgttcc atcaagtcgt tgtttgcttt taccatcgtt gtcattgctt gaacgcgtcc
    22981 aatagcatct gctaatgaat cagcattcaa tacagcatcg atgtagtttg agcttgtgtt
    23041 gcttacttgt gcttcgcgtg cttgtttttg gattgtatct tcacgttttt caatacgttc
    23101 ttgtaaatct gcaatgtctt ttactaattg tgcagattct tgacgcaatg tatcttgttt
    23161 tgctaatagg tcttgtgctt gtgtgttgat ttcagaaact tggctttcta aggcatcgat
    23221 ttgtgattgt gcatctgctt gttgattttt caagtctgca atcttttgat cttgctgttg
    23281 tatctgagaa tcaaaatcgt ccgccgcggc agcgatcggt gatgctacgg cagtcaaggt
    23341 cattgaacat accattactg ctgatattaa actctttttc actcttcatt cctccgactg
    23401 gcttaattta ataaatatct aaatatattt aagtctttct taacgatata aaaattgtac
    23461 catagcagtg tgtcaacgat ataatagtta tgttacaaaa atgtttcaaa acaactttta
    23521 aaaatgacaa aactgttcgt ttcagaaaat gatcatgaaa aagccgcgtt aagtctagca
    23581 tttcttaaga taacaacgat tctttttcgc tttcgaccta aaaataaaaa aagaaa
//
LOCUS       DAXLPI010000035        23575 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXLPI010000035 DAXLPI010000000
VERSION     DAXLPI010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.035"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACF3T5_001189"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="ACF3T5_001189"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFD3631812.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="ACF3T5_001190"
     CDS             complement(1172..1867)
                     /locus_tag="ACF3T5_001190"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFD3631813.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="ACF3T5_001191"
     CDS             complement(1880..2596)
                     /locus_tag="ACF3T5_001191"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFD3631814.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="ACF3T5_001192"
     CDS             2926..3534
                     /locus_tag="ACF3T5_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFD3631815.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="ACF3T5_001193"
     CDS             3859..4182
                     /locus_tag="ACF3T5_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFD3631816.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="ACF3T5_001194"
     CDS             4364..5734
                     /locus_tag="ACF3T5_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFD3631817.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="ACF3T5_001195"
     CDS             complement(5864..7165)
                     /locus_tag="ACF3T5_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFD3631818.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="ACF3T5_001196"
     CDS             complement(7519..9510)
                     /locus_tag="ACF3T5_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFD3631819.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="ACF3T5_001197"
     CDS             complement(9654..9920)
                     /locus_tag="ACF3T5_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFD3631820.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACF3T5_001198"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="ACF3T5_001198"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFD3631821.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACF3T5_001199"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="ACF3T5_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFD3631822.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /gene="def"
                     /locus_tag="ACF3T5_001200"
     CDS             13264..13827
                     /gene="def"
                     /locus_tag="ACF3T5_001200"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFD3631823.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="ACF3T5_001201"
     CDS             complement(14080..14601)
                     /locus_tag="ACF3T5_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFD3631824.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="ACF3T5_001202"
     CDS             complement(14604..15446)
                     /locus_tag="ACF3T5_001202"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFD3631825.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="ACF3T5_001203"
     CDS             complement(15439..16164)
                     /locus_tag="ACF3T5_001203"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFD3631826.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="ACF3T5_001204"
     CDS             16340..16636
                     /locus_tag="ACF3T5_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631827.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16892..17066
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17204..18796
                     /locus_tag="ACF3T5_001205"
     CDS             17204..18796
                     /locus_tag="ACF3T5_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFD3631828.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="ACF3T5_001206"
     CDS             18809..19549
                     /locus_tag="ACF3T5_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFD3631829.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="ACF3T5_001207"
     CDS             19717..21066
                     /locus_tag="ACF3T5_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD3631830.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="ACF3T5_001208"
     CDS             complement(21194..21784)
                     /locus_tag="ACF3T5_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFD3631831.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21931..23283
                     /gene="brnQ"
                     /locus_tag="ACF3T5_001209"
     CDS             21931..23283
                     /gene="brnQ"
                     /locus_tag="ACF3T5_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFD3631832.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6739 a         4755 c         4497 g         7584 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta acccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtatctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 atcaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acaaacttcc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgccatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 aacaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa aacagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagattact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct tttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagttaa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caagaactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccgtcgtt ttatatagga aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaccgtaaa ggagttatgt atgacaaaaa aattatcatt taaagactat
    21961 ctttttatag gctccatgct tttcggtcta ttttttggtg ctggaaatct tatctttccc
    22021 gttcatttgg gtcaagaagc aggcgctgct accttttggg cgaatctagg tttcttggtt
    22081 accggtatcg gtcttccctt cttaggggtg attgccattg gtgtatcgaa aagctcaggt
    22141 gtatatgaat tagcaaaacg gataaatcaa acatatgcga tggtttttac catcttgctt
    22201 tacttagtta tcggaccgtt ttttgcttta cctcgccttg caaccacatc tttcgagatt
    22261 ggtttagcac catttatcga tcctagcaga cagacgctat atctagcaat tttcagtatt
    22321 gtattttttg tgctggcttg gtggttttca agaaaaccga caaagatatt agattatgtt
    22381 ggaaagttcc tgaatcctgc atttttggtt ttattaggtg ctttgcttct ccttgctttt
    22441 ttgaatccac taggttctgt aaatcaagcg cctattcagc caaattatca agagcatgct
    22501 tttttcacag ggtttactca aggatataac acattagatg cgcttgctgc tttagcattt
    22561 gggatcatca ttgtcacaac aatccaaaac atgggtgtaa caaaacctgc tgaaatcgct
    22621 aaagacacga tcaaatctgg tgcgatcagc attatattga tgggaatcat ttatactttg
    22681 ctcgcttatt taggtgctat gagcttaggt agctttgctc taagtgaaaa tggcgggatc
    22741 acgctggctc agattgctga tcattattta ggtacttatg gcagtatctt attagcattt
    22801 atcgttatat tagcttgctt gaagaccggt attggtttga ttacagcttt tgcagaaaca
    22861 ttcacagatt tgtttccgca aagaaattac gcattctttg ttgcacttgc tagtatcctt
    22921 ccttgtttag cggcaaacgt aggcttgacg aatatcattc aattttcgtt gcctgttttg
    22981 atgttcatct atccattagc aatgacactg attttactag tgttagttgg tcctttgttt
    23041 aagcagcgta cggctgttta tcgaatgact acttatttta cattgattgc ttcgattttt
    23101 gatgggttga acgcttgccc agaaactatc aaacaaacac ctattgttca aaatctttta
    23161 tatgttgcgg aaagctactt gccgtttttc aaattgggga tggggtggat cgtaccagca
    23221 gttatcggtt ttgttatcgg actgatttgg agtttcgcta agaaagaaga agtagcagat
    23281 tgataaaaga ggttgtgaaa gagacgttca gccttattgc cgataggctg tctcttgaac
    23341 actgtttatt gagaggtcgt gaaaaagccg ctcagtttcg agtattaaga aatatttttt
    23401 gaaaatggct tctcacattt ttgcataaat ttgacttaat accgagaaac tggcttttga
    23461 acaccgttta ttgagcgtct ataaactgac ctgagtatta gagaaacaaa acggcaaagt
    23521 agctccatat actttgccgt tttgttttga actaataccg aagagaagct gatca
//
LOCUS       DAXLPI010000036        23399 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXLPI010000036 DAXLPI010000000
VERSION     DAXLPI010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.036"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(5..2155)
                     /locus_tag="ACF3T5_001210"
     CDS             complement(5..2155)
                     /locus_tag="ACF3T5_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFD3631833.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNN
                     NNGSNNSNNNNNNSNNGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPN
                     TANQSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFEHLGKPYVWGAKGPN
                     TFDCSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVG
                     IYIGNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPATSGSLTGSSILDGLDGS
                     FHFSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLF
                     GIKGSYNGSSVTFNTHEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDF
                     YKPTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEA
                     NAEILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQ
                     IPETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2512..3885)
                     /locus_tag="ACF3T5_001211"
     CDS             complement(2512..3885)
                     /locus_tag="ACF3T5_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFD3631834.1"
                     /translation="METTMNPEQMVLYEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRDNMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3885..4280)
                     /gene="rbsD"
                     /locus_tag="ACF3T5_001212"
     CDS             complement(3885..4280)
                     /gene="rbsD"
                     /locus_tag="ACF3T5_001212"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFD3631835.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4308..5225)
                     /gene="rbsK"
                     /locus_tag="ACF3T5_001213"
     CDS             complement(4308..5225)
                     /gene="rbsK"
                     /locus_tag="ACF3T5_001213"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFD3631836.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5334..6347)
                     /locus_tag="ACF3T5_001214"
     CDS             complement(5334..6347)
                     /locus_tag="ACF3T5_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFD3631837.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6428..7027)
                     /locus_tag="ACF3T5_001215"
     CDS             complement(6428..7027)
                     /locus_tag="ACF3T5_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFD3631838.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILSNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTNN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHVVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7212..7913
                     /locus_tag="ACF3T5_001216"
     CDS             7212..7913
                     /locus_tag="ACF3T5_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631839.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7923..10634
                     /locus_tag="ACF3T5_001217"
     CDS             7923..10634
                     /locus_tag="ACF3T5_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFD3631840.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNAKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11155..12783
                     /gene="treC"
                     /locus_tag="ACF3T5_001218"
     CDS             11155..12783
                     /gene="treC"
                     /locus_tag="ACF3T5_001218"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFD3631841.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12843..14024)
                     /locus_tag="ACF3T5_001219"
     CDS             complement(12843..14024)
                     /locus_tag="ACF3T5_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFD3631842.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYQNSSFSEEDYF
                     K"
     gene            14276..15799
                     /locus_tag="ACF3T5_001220"
     CDS             14276..15799
                     /locus_tag="ACF3T5_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFD3631843.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVASI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFITQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYIEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15868..16596)
                     /locus_tag="ACF3T5_001221"
     CDS             complement(15868..16596)
                     /locus_tag="ACF3T5_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFD3631844.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16609..17889)
                     /locus_tag="ACF3T5_001222"
     CDS             complement(16609..17889)
                     /locus_tag="ACF3T5_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFD3631845.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKTLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(18030..18632)
                     /locus_tag="ACF3T5_001223"
     CDS             complement(18030..18632)
                     /locus_tag="ACF3T5_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFD3631846.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18877..19083
                     /locus_tag="ACF3T5_001224"
     CDS             18877..19083
                     /locus_tag="ACF3T5_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631847.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19395..20114
                     /locus_tag="ACF3T5_001225"
     CDS             19395..20114
                     /locus_tag="ACF3T5_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFD3631848.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20195..20878)
                     /locus_tag="ACF3T5_001226"
     CDS             complement(20195..20878)
                     /locus_tag="ACF3T5_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFD3631849.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21124..23310
                     /locus_tag="ACF3T5_001227"
     CDS             21124..23310
                     /locus_tag="ACF3T5_001227"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFD3631850.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7794 a         4219 c         4592 g         6794 t
ORIGIN      
        1 tattctattt ctttaaaggc agatcaagcc agaatgattt gtctaaactt aacaaggtta
       61 gtacaggaac agttgcagct ttaactgtac gagtagttgt ttctggtatc tgtcttgccg
      121 taaaattgtt ccacaaatcc cagattgaag cgacacggaa gatggaagaa gcgttgttga
      181 cgcgttttat cgttgaaaat tctaatcctg tgattggcac gccatctgtt gtccatttga
      241 ttggcacatc gaatcgctta gacatattga ggatttcggc gtttgcttct tcagttgttg
      301 ctgcttcttt tttcggttca tcatattttg tcaggtcgta cgcttcgatg atcgcgttca
      361 atttttgtga atattggcga tctgtagcat aacgtccttc caaatatttg gttgcttctt
      421 tgtagtcttt tgtttccgac ttccaagttg gtttgtagaa atcaggattg ccagtcagtc
      481 catttttgat caagtcggca tagtcttcca aactttcctt gtaagaagga tatttacgga
      541 attgtgcgcg gatttggtag ctttgtccgg aactatcttg ttcatgtgta ttgaaggtca
      601 cgctgcttcc attatatgat cctttgatac caaaaaggtt atgatttggt tcgcttgata
      661 ataagctgtt tccagatcca ctttcaagga tagcttgagc catcatgact gatgcgtaga
      721 ttccttcttt ttgcccgatt tcctgtgcat catctgcaat ctttttcaag aactgatcag
      781 ttgtttggtt ttgactgaaa tggaacgagc catctaatcc atctaaaata gatgaaccag
      841 ttagcgagcc tgaagtagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
      901 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttggggcgca tggataaatt
      961 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
     1021 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
     1081 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
     1141 aacagtcaaa agtatttgga cctttggctc cccatacata tggttttcca agatgctcga
     1201 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
     1261 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
     1321 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
     1381 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
     1441 tcgaagaatt cccgttgttc gaattattgt tattattgtt tgaattattt gaaccattat
     1501 tattgttgtt attgttgtta ttacccacat ctggtttgtt tggcgtttcc ggatctggtg
     1561 tactagggac ttcttcacta ggatcagttg gtttctctgt tgaagtatcc ggtacaggtt
     1621 caaattctgc agtaatagat aaattgttcg tacctactgt aaaggtcttt gcatcataat
     1681 ttacttcttt ggcaccatta acagcgactg aagcaatttg ttcaccttca gctggcgtaa
     1741 tagtgtaagt taccttcgtt tccgctaata aaccttctac cgttccactt tccgtaatat
     1801 ccaaaggttg ctgttcaggg gaatccgctg tcgaagcagt cagttggatc ttaccaatca
     1861 aagaaggagc aattgtaagg gaatatgtct cggctggctt ttcattttct gatggatcag
     1921 ccggatcaat ttcagttggc tcggtcgatc ctgttgttga gttagttgtt ccagttgggt
     1981 cttctgtaaa agaatccgtt gtttcactag tagctgagct ggatgtagtc gtatctgttg
     2041 tgactgtttg atctgctgtt tgattatcta cggcaaatac ggtattggtc gacaaaacgg
     2101 acggagcaat cagccaagta gcagctagca aacgtatcgt ttttttattt ttcataataa
     2161 actagtcctt ttcctttcat tgattttctg tcttattcag cattcattag acattttttg
     2221 gaacagataa tcgtgtcgaa aagctttttc ttttcataaa tctgtctgtg tattctaaat
     2281 aaaacaattt gtaaataagc aacgagcatt cattcgtttt tctttgacgt tcattttatt
     2341 gcatagacca cctttgtttg gggataaaca agtggggata aaaatggtta tgcatatgta
     2401 aattatacca aagtttggaa agtatcacaa aaatttataa aaataatgat cctaaaaaga
     2461 aaaagactga aatatgtgat aaacatctca tacttcagtc tttttcatta actattcata
     2521 agacattagg tcttcatcaa acccgctaac gagagtagta atcattgcta cgactagact
     2581 aacagccagt cctgctaagt accagaaaaa agtaccggaa atgaatgcag gcaatgtggt
     2641 cagactacca aatacaaaag cgatcgcttt cacttgggca aatcccatta cagctccacc
     2701 agcagctgca ccaatgcaag cagcaacaaa tggttttttc aatgggatcg caattccgta
     2761 aacagttggt tcggtcactc ccgcaagtaa aatcgaagca aaagcagaac cggagatcga
     2821 acgagttttt gaacgtttac ttcgtagaaa tacacctaaa gctgcaccag ccattccaaa
     2881 attggctgct cctgtcaacg gaacgattcg gtcgaaccca taagcagaga tattactgat
     2941 catgacaggg tttacagccc attggatacc aaatattacc aaaatattcc aaccaccagc
     3001 gatgattgcg ccgctgatcg gtccgttaat atccatcatc caagtaattg cggcagctaa
     3061 accttcgcca aggtaaacac ccgcaggccc gaataccatt aacgtcaaag gaaccgtgat
     3121 aactaatgaa agtaaaggta caaatactaa ttgcatatta tcgcgaatat attttttgag
     3181 gaatttttca acaaaagaat aaaagaaaat acctaaaatc gccggcatga cagtagacgt
     3241 atactgcatc agtactacag gaatccccag aaaatcagta gctgctttgc cactgtctga
     3301 aagtaaagag gtcagattcg gttcaatcag tgctgcagca atgacagccg caatatatcc
     3361 attcacttta aacgttttgg cggcagaaaa agcaagaaag attggcagaa aataaaagac
     3421 ggcattcgac gcagcagaca aaatatgata agttccgctt gtttcactaa gccagccaat
     3481 cgtagtcgct aaaacaacca gaccttttaa tataccagaa ccggcaaaca gcggaatgat
     3541 cggcgtaaag atttccgata tcaaaccaaa tagtttttcg atcggattta gcttttcttt
     3601 cggagctgaa tgatcgattt gacgcgtcaa ttccttgtaa gcaggagcca atccctgatc
     3661 catgattatt tctgcatatc tatcgagtaa aatgaaatac ttaatggaag ggatttgctg
     3721 ttttaatttt tctgcattgg ctactggctg ttttttcaat ttgatacgaa gccgagtcgc
     3781 acatggaagg acagaatcaa tattgtcttt tcctcccaac tgttcaatca gcatatgaat
     3841 ctgctcgtac agtaccatct gttcaggatt cattgtcgtt tccattagaa gaccactcca
     3901 ctcactaata taatattggc gtaaggtgtc atttcgcctg tacgaataaa tgcatgacag
     3961 ttattcaact cttttttcat ttcttcatgt gggataaagc taatcggtgt atctggtaac
     4021 aataatttga ttttttccaa tatttccgga ttcattgtct ttatttcttc cgccaattca
     4081 atcgattgta cctcaagttc ttctaaaacg ttttctaaaa cagataaaaa gcttggaaga
     4141 ccgtttgaga cagccaaatc tattttttca gtaccaaagg ggacaggcat gcctgcatcc
     4201 ccgatactta atttatcaaa gtgccccatt tgtgcgatta ctcgtgaaat atctgaattg
     4261 attacttttc cttttttcat gtttgtttct tcctctcatt gttaatttta gactgctgat
     4321 tgttctaaag cttcgcggta agggatagaa ggttgcgcac cataagactg cacagcaaca
     4381 gaagaggcta gatttccata aaggatcgct tctttcaagt tactaaggtc tggctgaagt
     4441 gtgctgctta atgcgccaat aaatgtatca cctgcggctg ttgtgtcaac ggcatccact
     4501 ttaaaagcag ggacgatacc gtgttctttt tctgtatgat agaaagcgcc ggcactgcct
     4561 agtgtaatga ttactgttcc aatacctaat tcatggagtt tttcggctgc tgcgaccaaa
     4621 ctgttatgat ctgtcactct gattcctgta atgatttctg tttctgtttc atttggtata
     4681 atgatatctg tcttttcaag tagttcagta ggaatcgttt cacgagctgg tgcaggattc
     4741 aggatcgttg tttttcctgc gtcttttgca atagaaaaag ctaggatcgt cgcatctaag
     4801 ctactttcaa actgagcaat aataaagtca ctgtttgtaa tcagttgctt gttttttagg
     4861 acttcttgtt catggaaagc attatttgcc cctgcatgga tcaagatact attttctcct
     4921 gctgcgtcta ccataatcat tgcttgtccg gttgtggcgc cttggatttc agctatccca
     4981 gatgtatcga tgttttcttt tgtcagtaga tctaataact gttgtccttc gctgtcagcc
     5041 ccaacgcctc caataaaaga tgtccgtgcg cctgaacgtt tggctgccac tgcttggttg
     5101 gcgcctttac ctccgccagc gtgaaaaatc tcatgcgcat gcatcgtttc gcctggcttg
     5161 ggcatgttgg ttaaacgaag ggtagtgtcc atattaatac ttccaattac tgtaaccgtg
     5221 ttcattttac aaacctccaa ctatctcatt tattttgatg ggaaaaatta ttttttaaaa
     5281 cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc
     5341 aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct
     5401 ttttctggga attggatccg atctaacaat aatttggcag atgtttgtcc caattcgaag
     5461 atcggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca
     5521 tagccaataa tgctgtagtc ttcagggatg ctttttccag attcctctaa tccgcgatat
     5581 aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt
     5641 tctgctgtga tttggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg
     5701 tcgtgagaga tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca
     5761 ttttccggcg gattttcagg atagacaagg gcgatcgttt gatgacctag actaagcaag
     5821 tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct
     5881 tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg
     5941 attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg
     6001 agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg
     6061 acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga
     6121 ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta
     6181 gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt
     6241 ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg
     6301 ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta
     6361 tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg
     6421 ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg
     6481 cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc
     6541 aagcatcttc agcaagtacg acatgatacc catgttggaa ggcatctaat gcagtagcat
     6601 atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa
     6661 gttgaagatc aagagaagta ccaaaaaagg cacttggatt gtattttgtg atagtcaaca
     6721 tattgtttgt atctttcaag gaatccttca acaacaagtc agtgaattct ggaggtacag
     6781 aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta
     6841 cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt
     6901 ttgacagaat agattgtgca gtgtgtggag agagtgtacc gttatttacg atcccttttt
     6961 gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat
     7021 cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg
     7081 tttatttgtc aactaaataa aggattcctt tctttagtct agagaaattc agtggtaaaa
     7141 taaataagtg tgagaaaacg cattctatgc atagatatag cgagaaatat cgtgaaaatg
     7201 gaggaatcag agtgaagtat gtcgaagtaa aaaatgaata caaacgatac aaaatgggag
     7261 aaacgaccat tacagcaaat gacgatattt ctttttcaat tgaaaaaggc gaattagtga
     7321 ttatacttgg tccaagtgga gcaggaaagt caacgattct taatatttta ggtgggatgg
     7381 atacacctga tgaaggtcag atcattattg atgacacaga tattgcgcaa ttttcagata
     7441 aacagcttac tgcttaccga cgaacagatg tagggttcgt tttccaattc tacaatctcg
     7501 ttcctaatct aacagcaaaa gaaaatgtgg aattggcaac ggaagtttct cctgatgcgc
     7561 ttgatcctgt agaggtattg aggcaagtgg gcctagctca tcgattgaat aactttccgt
     7621 cccaattatc aggaggcgaa cagcagaggg tttcaattgc tcgtgcacta gctaaaaatc
     7681 ccaaacttct tttatgtgat gaaccgacag gcgcgctgga ttttgaaacg ggcaagcaag
     7741 tattgaaatt attgcaaaac gctagcagac aacacggcaa taccgttttg atcattacac
     7801 ataattctgc tttagcgccc atcgctgata gagtcattca cattaatgat gctaaagtaa
     7861 ggtctgttga attaaatgat catccttcat ccattgatga gattgtatgg tagggggatt
     7921 ttatgaaaac aagaacatat cttaaagcag gttttcgcga aattcggcaa tctaaaggac
     7981 gattcattgc aatcatcctt attgtcatgc taggaacact tttatttgta ggtgtcaaaa
     8041 ctgctggacc tgtcatgcaa aaaacgatgg atcactatgt tggtacagca ggactttctg
     8101 atttgcaaat cgtttctact ggtggcttga cacaaaaaga tattgctcaa gcagaaaaga
     8161 tcccagatgc aaaagtagaa acaggaaaac agatctatta ttccaatact ggcaaaaacg
     8221 aagtgattca aatattttct tataacaaaa ataacaagca gaacaagcta caattaactg
     8281 atggaaaact cccggaaaaa ccaaaccagc ttgtattgga tgaaaaagcg gaagatgaag
     8341 ggtataaaat cggtgatatt tatcagattg attcagatga attgaaagaa aaagagtata
     8401 ccatcacggg ctttgtccgt tctccattgt tcatcaacaa tttggagaga ggatacgcga
     8461 acgtcggaaa tggtagcgtc gattattttg tctatttacc tgaatccgtt tttaaaacag
     8521 atatttattc tgtcttatat cttgatttta caaacgcaaa gaatttgaat acgtacagca
     8581 aagcttacaa agataaaatg gagcaaaatc aagaaaaggc agaaaagtat ttacaagatc
     8641 gacccgaaga acgcttagaa gagctgaaaa aatcggccaa cgaagaatta gaaccggcac
     8701 agcaaaaaat agcggatggc aaagcacaaa caacgcaagc aagaacacgg ttagaagatg
     8761 ctaaaaaaca gttagcgcaa cagtcagcaa ctatccagca gcttcctgaa gcgcaacgaa
     8821 caatcgctgt tcaaacatta tctcaacaag aagctcaact tgctgaacaa gaaaggcaat
     8881 tagttgagaa agaacgtgaa ctggcctctg cagaggaaga attgaatcga aatgcagaaa
     8941 aaattgaaaa tttaactaaa ccatcatacc tatacaatga acgttcagaa aatgtcggtt
     9001 ttcaagaatt tggtgatctg tctgatcgta tcgctgcgat tgccaatgtt tttcctgttt
     9061 tctttttctt tattgctgca ttgatcacct ttacgacaat gacacgtatg gtagaagaaa
     9121 accgcaggga aatcgggaca ttaaaggcgt taggttatac gaaagttgaa atcgcaaaaa
     9181 aatatgcaat ctatgcaagt ctagcctcag ggataggcat tgttttagga acgatattag
     9241 gaacgaatct tttgccaagg atcatttttg agctgtctaa cgagcggtat gatattggga
     9301 gtgcgcttat tttttatgac tggccgccga ttttacaagc agctgccgca ttcttcattg
     9361 ccgcatttgg cgctgcaatg atcgttttat tcaaagattt aagagaacgg cctgctgcat
     9421 tgcttcaacc gaaagcaccg aagccaggga aacgcatttt tcttgaatac atcactccgc
     9481 tgtggtcacg ccttagcttt aatcaaaaaa tcagttaccg aaatctcttc cgatataagt
     9541 caagaatgct tatggcgatt atcggaattg ctggatgtgc cgggctgatg gttgcaggcg
     9601 tgggattgaa agattctcta agttctgtct cagccaaaca atttggaccg atcattgact
     9661 atcaagcaat cgttacattg gattcatcca cggaagatag tcgtcaaaaa gcagcggata
     9721 tgctgcagaa gcaagaaaaa gtaaatgaga tcctagctat tcatactcaa accgtagaat
     9781 tgagaaaaaa aggacatgct tcgcaaagtc tgacagtgat cgtaccaagt aaaacagctg
     9841 atttggatcg ttttatccat ttaactgatc ttgaagagaa cccacgctcc ttgcctaaaa
     9901 caggaatcgc tattacgcaa aaagcagcag aactatttga cttatctaca ggagatcata
     9961 tttcgcttta tgatgaagat caaaaagaat ggaaagtcga agtaaaagaa atcgtgaaaa
    10021 actatctagg tagctttatt tatatgtccc ctgaatatta tgcttcagtt actgatcaat
    10081 cgatgatgga taatgcattt ttgctgcgta cagataagat gacaacaaaa gaagaagaaa
    10141 tgctttccga gaaactgctt gccacagacg ctgtgacaaa tacgtctttc atgtccaaac
    10201 aaatcgaaac gcaagaagaa tcaatggcga acttagactc ggttgtctta atctttgtcg
    10261 tattatcagg actgctggct tttatcgtct tgtataatct aactaatatc aatatttccg
    10321 agcgtgtacg cgaattatct acaataaaag tccttggatt ttttgataaa gaagtcacga
    10381 tgtacattgt tcgagaaaat atcatcttta ctcttttagg aatcatcgga ggatttggtg
    10441 taggatatat attgactgac ttcatcttgc aacaagcgtc tatggagaat gtcatctttc
    10501 cattagtgat cacatggtgg gcttatgtct tatcagcagg attaactatc gtattcacgg
    10561 ttattgtgat gatcgttacc catttcaaat taaagcatat cgatatgatt gatgcgttga
    10621 aatcgaatga ataaaaacag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    10681 gccaaacaaa aatagcttct catatttttc gtttgattgg gcgtattacc gcagatgcaa
    10741 gcttttaaac accgcttaag gaagaggttg tgaaatcagc gttttgagct aataacgagg
    10801 gtcagctgct aattgaacac cgtgaatcag aggttgtgaa aaagctgtcc tgcatcgagc
    10861 aaaaagaata ctcaaataaa attagctcct catgttttac atttgagaag actgattgcc
    10921 gtagataaaa gcttttgagc accatttata gaagaaacaa gaatattttg ctattttaaa
    10981 caagaggata gacaaataca tgtctgatct cttgtttttt attgggcaac agacctctta
    11041 gaatgtataa agataaattt tacaaacgct ttcggttcag ttataataat aaatgataac
    11101 ttgtatatat aagttttggt aggtaataaa tcagttgaat ggaggtaaat atccatgaca
    11161 tttaaagaca aagtcattta ccaaatctat cccaaatctt acaaagacag taatggtgat
    11221 ggaattggag atttaaaagg aatcaaagaa aaactcccgt atctgaatga tctaggcatc
    11281 gacatgattt ggctaaaccc tatttatcca agccctcaaa aagacaacgg ttatgatatc
    11341 tctgattaca cagccatcga tcctatcttt ggtacgatgg aggaattcga acagctaata
    11401 gccgaagcaa agaaataccg gatagaaatc atgctcgata tggtgctgaa tcacgtttcg
    11461 acagaacatg aatggttcaa aaaagcacta gcaggggacc agcattatca ggacttcttc
    11521 attttaagag aagagccaac ggattgggta tcaaaatttg gtggaagtgc ttggtcgcca
    11581 tttggagata cggggaaata ttatttgcac ttatatgatc ggacacaagc tgacttgaac
    11641 tggcggaacc aagcagtaag agaggaacta ttcaaagttg ttcgtttttg gatggaaaaa
    11701 ggggtaaaag gattccgttt cgatgttatc aatgttatcg gtaaagacga acaattgaaa
    11761 aataataaag aaaatgatgg caaacctgaa tatacggaca aaccaatcac ccatacgtat
    11821 ctaaaagaat tgaaccaagc aacgtttggg acggattcca ctatcatcac agtcggtgaa
    11881 atgagctcta cgacgatcga aaattgtatc ttgtatacag aacctagtag agaagaatta
    11941 tcaatggtct ttaatttcca tcatttgaaa gtcgattatg aaaatgggaa taaatggtct
    12001 actcctccat ttgattttgc agaattaaag cgactatttc atacatgggg agaaaagatg
    12061 agtgagggta acggctggaa tgctctattt tttaataatc atgatcagcc acgtgcgctc
    12121 aatcgatttg tagctatcga tcagtatcga aaacaaggag cagagatgct agcaaccatg
    12181 atccatttga accgtgggac gccctatatt tatatgggag aagaaatcgg catgatcgac
    12241 ccagactatt catcgatttc cgactatatt gacgtagaaa gtttgaatgc ttataagtta
    12301 ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc ggatcaaagc aaaatctcgc
    12361 gataactctc gaacacccat gcaatggtca gacacagaac aagcaggatt tactacaggg
    12421 aaaccttggt taaaagttgg aggcaagcta gaagagatta atgtagaaaa agaactatct
    12481 tctgaagatt ctattttgtc ctattacaag aaattgattc ggctgagaaa aacatatcca
    12541 atcgttgcac aaggagatta tcatgcttat gaagcaaatc atccggaagt ttatggcttt
    12601 cttcgccaat ttgaaggaca gcagctgctt gtattaacaa atttctatga taaagataca
    12661 aggatcgcca tcccagaagg attcacagaa gccaatattc ttatcgataa ttattccgat
    12721 actgttcttc atcaaagtct ggagctaaaa ccataccagg caattgcttt gctgaaaaat
    12781 taaaacaaaa acacaaaacg tcgttcctac gagctattct cgtaagatcg acgttttgtg
    12841 ttttatttaa aataatcttc ttcagaaaat gaagagtttt gatactctcc tttatcgcca
    12901 taaaatttat tgtagcgttg tttgaattta cggaaaataa aagtaacgag taatttgact
    12961 aacgtataga taggaatacc aaaaataacg cccacaattc ccattagatc acccatgaca
    13021 agtaaaacga ttaagatcgt aatcggatgg atctgtaaac gatcacccat tactcgagga
    13081 atcaccaagt caccgtggag aagttgtacg gcaaaccaga caatgacaaa tttgacagcc
    13141 ataaaagtag agtcttggaa tgcaatgatc aaacctggaa agaaggcgat aaacggccca
    13201 atatatggaa tgatacataa caacccagca gaaatagcta aaacactagc aaattctaat
    13261 ccgatcagta agaaacaaac ccagtagact gctccaagga taaatgaagc aatgatttgc
    13321 ccctttagaa atgaacccaa ttgttgatta gcaatctttg tcaaattttt aaaatctgta
    13381 cgaaatgctg gtgggacaat cgctaaaact gactgataaa atttttgagg atctctcaaa
    13441 aggaaaaaag cgacaattgg accagtaaat aaagtaataa aaatagctgt aacagttgaa
    13501 aaaatgttgc tgatccgttg tgcgccactt tcccaataat ttccaataaa tccagctata
    13561 tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa atgaatcggc aaaaggtgtt
    13621 ttctccaaaa attgtcggag actctcttga aaatcttgaa acaaatctgg caaactactg
    13681 atcaatgtct ttgcctgttc ttcaagaaaa ggaacaagcc atacgcttcc aagccccaat
    13741 aaaaataaaa taatcacata aacgataatg actgacaagt tcctcgaaag gtgcttgctc
    13801 aaacgtttga tgactggttc caaaagataa tacaagatca gtgcaaacaa taatgggggc
    13861 aagatagtag cgataatgat tccgacaggc ttgaatataa acgagatctg atggaaaaaa
    13921 taaatgatca gcgcaagtaa gataactatt cctaaaacat agtaagaggt ttttcctcct
    13981 aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt tcaatctttt tcctccttta
    14041 tctataactt cattatagac aaaagcgggt ataaaaggaa aatgatagag gttgattgca
    14101 aaaatatcgt caatcattca cataaacttt ctcatcaatt aaaataagaa aacgcttgac
    14161 agttatttta accatatgat aaattaaatc ctgttgctaa aacgtaacta ttacggttta
    14221 caaaaggcga atcggaacat tcaaatgaaa gaagatgaga agaggagtgt cataaatgaa
    14281 acgattgata ggtatattgg ccatgctggt tttagcaggt atgttaactg cttgcggggc
    14341 aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg atgacgacct tttatcccat
    14401 gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa gtagagttat tgattccagc
    14461 agggactgat tcccacgatt atgaaccttc tgctaaagac atggcaaaga tccaagatac
    14521 agatatcttt gtttataatg atgaaaacat ggaaacatgg gtccccgcaa tccaaaaaac
    14581 attacaagaa ggcaatgtcc atacgataaa agcgacagaa ggtatgcttt tacttccggg
    14641 aagtgaagaa ggacatgatc atgaccatga acacggggaa gaaggccata cacacgagct
    14701 agacccacat gtatggctag caccaagttt agcaatcaag caggtagcaa gcatacgtga
    14761 tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg acaaaaaatg cagcagccta
    14821 taccgagaaa cttcaagcac tccatcagct ttatcaagaa acctttaagc aagcaaaaca
    14881 aagatctttt atcacgcaac atgctgcatt caactatctg gcattagaat atggattgaa
    14941 tcaagtatcg attgctggtc tttcttctag tgaagaaccg tctgcggcga gaatcgctga
    15001 actgaaacac tttgttaaag aacatggaat caactatatt tattttgaag aaaacgccaa
    15061 agatagtatt gccagaacgt tagcaaatga agcaggtgta tctttagaag tattgaatcc
    15121 tttggaagga ttgacgaatg aacaaataga aaatggagaa aactatcttt ctatcatgga
    15181 agcaaacctc gaagcgttga aaaaaacgac agagacagaa aacccgttag aagatacttt
    15241 gacccccaaa aaagaaaaga gcgtctataa cggttatttc gaagacgagg atgtacaaga
    15301 tcgctctctt tcagattggg caggaacttg gcaaacagta gatacgtata tagaagatgg
    15361 gacatttgat cctgtttttg aatataaaga aaaattgaat caggataaga cagcacaaga
    15421 atataaagaa tattatacga agggctatca aacagatatt gcttcaatca agatcagtga
    15481 gaaagagatg gcatttacgt ttaaggatgg aacaacgaaa cgttcaaatt atcgttatgt
    15541 agggaaagaa atcttgacct acgaggcagg aaaccgtggc gtaaggtatc tattcgaagc
    15601 agaagataag gaaagtggcg cttttagata tattcaattc agtgatcaca cgattgcacc
    15661 ggctgactcc gaacattttc atttatatgc aggaaatgaa agtcaagaag cattgctgaa
    15721 agaaatggag aattggccaa cattttatcc agatacaatg gatggacgtg aaattgcaca
    15781 ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg gtttggatcg gctggtcatt
    15841 tagtcggtcc aaactattcg gttcttttta tgaaatcaat ttctttaatc cacggataga
    15901 ggctgggatg gtctcgccgt ttgccaacgt gacttctctg gtttttttat taatcgaccg
    15961 cacattttgt gtattgataa cataggattt atgacaacgg ataaaattcc gatgcatttt
    16021 ctcgatttct ttgatcttct catagaattg taattgggca tgagtcaaat ggacttccag
    16081 tttatgtggt gctcctagcg aagtagaaaa atattggatg tctttgatcg gtaaaaattt
    16141 gatctcatca ttaaactcta ttttgaagat atctttttgg atctgggaca tttgaaaatc
    16201 ttcgaaaatt ttgctcagga tatgctcgac attttctttt aaatcataaa gatcctcttt
    16261 gacgatataa tcagtcggtt caatgttgct ttttaagatt ttcatcgaca tttccgtatg
    16321 ggaagtcaca tagattattt ttcctaaagg atctaattca cggatcaatt taccgatcgc
    16381 tacaccatcc atttcactgt cttctagttc gatatctaaa aagtaaatac caaaagaaga
    16441 agttctgatc ttggctgcat tgagaagttc ttccggatca gaggtagaaa tatggacata
    16501 actgtctaag ttttcaatca tgattctatt tttgatcacc gttgataaaa cttctaattg
    16561 ttttggatcg tcttcacaaa tataaatggg aatcatatca ttactccttt attttatttg
    16621 tatataaatc gtttgagaaa aacgctggtt ttcaattgat gtttcaagga aaagataagg
    16681 attggtcttt gtcaattcat ccaagataaa caagccaagg ccttcgtttt ttggatttgt
    16741 ttttgttgag aacccttttt gtttcaactg gtttaacggc aatgtatttg ttgttgtatt
    16801 gatcacacga atcattacgg aatgattctt cttgagtatg gtcaattcga tttttttctt
    16861 ctttgcagca ctagctcctt caatcgcatt gtccaagata ataccaagca tacgtacaag
    16921 gtttaccgta tgtttcgtat ccagttcgat tggctcagga aaatgaagct ggacgaccaa
    16981 ccctttttcc tgtgccatca tgagttttaa tgacagtaaa ctacggatct caggaacttt
    17041 catattatcc aacatactta gattggccgg aaaaaggttc atcatctggc gtgtaggtaa
    17101 tatcgtttga tcataataag accgtaatcc ttctaagtcg ccttctgaaa tatagctgtg
    17161 aagagaaaaa agcagatttt ggtaatcatg tttgaattct ctcaagtctt gatagttttt
    17221 ttctagatct ttgatataat cctgttgcat cctgacttgt tgctctaata actgatcagc
    17281 taatttctta ttagagattt tccaataata aaccaagatg atcaggccaa cacacagata
    17341 tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt gtttcaccag aaaaacgggt
    17401 ataaaaaata atgatatagt atgtaaataa tatcaatgtc ccgatcgtac cgatcatacg
    17461 gcggtccaca gtgtgaaacc aagtctctaa cgttttttta aaacaaaaag ctaataaagc
    17521 agataaaaaa gcggataagg caatgtgaaa atactgcaat gaatctcctg gttcaataac
    17581 cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag tgatctccta aaagcgacgt
    17641 caataaccca aaaggaaggg cgtaaaccaa ttgttgacgc gaagtcgaaa agaagaaaaa
    17701 agtaagcatc agcattccta tagcaattag agcagtaaaa atccctaaaa tcgaatagac
    17761 ccatccaatc aaaatacaag aaatcagaat gactcccgca aacagatatt tgtatcggat
    17821 gtgatggtca atcaataaga tacaaattgt gtaaatcaaa tattgcaacg ctatggacca
    17881 taaaaacata aagtacagct cccattgatt tttaccaaat tatagcacaa ttagcgtatt
    17941 atttttttaa tttttcaggg attttgaatt cagaactcat accaaagcag cagccagata
    18001 caaaaaagtc agataagtta gttaattgtt taatgattgt ttttttcatg aaagattcct
    18061 ccaaaattca tcaagtagtt tgtaatagga agcaacgtag ctatttctaa aacaatccca
    18121 agtagcaaat aggtagaaaa gtaagaagct gagaaaaata aagtaatgag agaaaaaatt
    18181 agaaacatgc tgatcgcctt ctttaataat gggggacgtt tctcgggttc aatgggattg
    18241 attgctgtac ctcttgatcc atagcgaagc agtaacaaaa agcagctgat taacaaaaag
    18301 accatcattg ggaaggagat agtcaattgc agatgtttca tcaccaaggg aacgccccat
    18361 aaataaagta gtgtccacag cagacaaacg aattcagagt cggaatgcaa tccataagca
    18421 tatctgcgga tgcagatata agaaagaaag gtgattcctg tttccgccca tgagcctgtc
    18481 accagagaaa agactagaac gaccgctagt tttccaagat tattcaataa aacttctaag
    18541 gcaaatttta cttggacata caccatatct tcttccgatt tgtctccgct gaataaggct
    18601 tctaataatt tgttcacaac tttatctttc atctcatacg tacctccacc acaaatttta
    18661 tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag tggttttgac atcaaaaccg
    18721 tcatgatttc ctaaaaaaat cttcgttttc caagaacctt accaaaaaaa gtgacagttt
    18781 tgatacgaaa agtacttttt atgacaagtt tgtgcttttt cgagtgattt atgttaaaaa
    18841 attactgacc ttataaaagg tgatggaggg attcttatgg aaaatgaaca attgtttgaa
    18901 gtcggcgaaa catatgcatt taaatctgat ttatttgaat caccaataaa aggaaaaata
    18961 gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga aatgcgcagt agaagacaat
    19021 gataccgctc ataatctgca ctggcagtta gttgtacgca aatcaagtac aatagaagaa
    19081 taaaaacaga taaaatgttg agagcataaa gaggttgtga gtctcccgtt tagctctgag
    19141 ttacaaggaa taatgttaaa aaacagcttt tcatgtttta tgaatattgc cttgtgaccg
    19201 aggtgttggc aggagaatac tgtttatttg aaaaaagggc tgcgacaaga cttttgtcac
    19261 agcctttatt ttcatgaata ggagttactt ttggctactt ttagagaagt agcatttaag
    19321 gagcagataa agtcaaggct tttatttcta taaatgaagt agtaaaatga agaacagatt
    19381 ttattacagg aaggatgccg ataattgtga tcattgttgc aggaacgatc ggtgcaggga
    19441 aaagcacact gactgaaatg cttgcccaag atttagaaac aaaacctttt tatgagaacg
    19501 tggaagataa tgaagtattg ccgctatttt attctaatcc agaaaagtat acttttttat
    19561 tacagatttt ctttttgaac aaacgctttt tggcaatcaa agatgctttc agccatgatg
    19621 acaacgtact agaccgctcc atctacgaag atagtatgct gtttcatttg aatgcagatt
    19681 tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg tcttttagaa acgatgctga
    19741 aggagttaga agaaatttcg ccacaaaaga aaccagatct actggtttat attcgtgtat
    19801 cctttgaaac tatgttagcg agaatcaaaa aaagaggccg ggaatatgaa cagctagaac
    19861 aagatcctga actctattcc tactataaag aactcaatcg acgatacgaa gagtggtatg
    19921 aacagtttga tatatgtccc aaaatagtga tcgatgggga caaatatgat ttcgtagccg
    19981 atccggcatg tggagaacaa atcgtacaag aaatcaagat gcgggcaaag aaaatgacag
    20041 aagaggcaga tgcccagtcg atccgctaca ctacaaataa cccgaaagca aagggaatct
    20101 ttacaactaa ataatataaa aactaagcaa attgctcact cgcagatatt tctctgtgag
    20161 cagcatcttg cttagttttt ttgattcatt gagatcaaat agtgaaccga ttcgtatgat
    20221 aaaattgcca taaatgaaat gtctgagtga gggccatgat caaaaataaa atggaaacga
    20281 tcaaaatcga atgggcgcca aacagatgat gacagaaaac aaggacaata gctcctgcga
    20341 taaaggcaag aacgacactt agatagtgga aaaatcgatc aaaatctgct ttttctttag
    20401 aaatacaata atcatataag ttcgttgcta ccccttttaa atttccagtt gtaaataaat
    20461 tagtgtaggc acgcccgttg attttatcaa aagctaccca ctggatcgat gcagtaaaag
    20521 caatcaaaat cgttactaat gcgggatgct catggacggg tgtcaaacta acaaagaaaa
    20581 aaatcactgc ttcataatat agaacaaaca agcgccagaa atgtttttct ttttttctaa
    20641 aataatcaat cagatacttt gttagaagaa tccctaagaa aaagaaaagg gtagacaata
    20701 cttttttacc aacacttagc cattctccgt caaatgcttg gatgatcgat agaatgatat
    20761 tgccagtttg agcagaggca aaagcaccat attggatata agtataactg tccatggctc
    20821 ctccgataaa tgtcagcaaa agtcctattg tacgcccctc gtgaaaggaa gtctgcattt
    20881 ttttcatcac cttttatgaa tatttttgtc ttccttatct tagcataaac gattgaaaaa
    20941 aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc attttcattt ttagaaaggt
    21001 ctgttagtat aaagaaaaaa tagacaacaa aactttaaat aaccaacttt aaataaccaa
    21061 ctttaaataa ccaacttgaa ataaacaaat tcaaagaaga aagatgggaa ggaaatcaat
    21121 cagttgaatg aaaaagaaat tctccgatta caaaaagaat tgaaacgctg ggagaacaag
    21181 tataaaaagc ttcaacaagt ctttcggcta caagaagaaa tcattgctac ctatgaagcg
    21241 gcttatggcg aagcacccgg acttattgag gaagaagtac aagtaaaaga aagtgaagga
    21301 aacagctctt tctttaaaat gatggagaaa cgttcctata atcgcttcaa tcaagaacaa
    21361 aaagcagcta tcacatatga tatgaagaaa catcttcgaa tcatagcagg agctgggagc
    21421 ggcaaaacgc aaacaatctg tgcaaaagct gtttatctga tgacgcaaaa acaagtagac
    21481 gaagaacgca tactaatgat cacttttaca aggaacgctg caaatgaact aaaaaaacga
    21541 gtagataatt tcagccagag aaaaaccgct gtccatatcg gaacattcca tagtatcttt
    21601 tttaggatat acaatgaaat ttgtcgaaaa tttcctgaag tagcaatgca aggaatacaa
    21661 ggagactttt caaaggatac tgcacaaaaa gtgaatgctg ttttgcaaca gctgatacgt
    21721 aaatataatc tttatatatt tgatcaatat ggagaaaaaa cgattgcttc tcggttagac
    21781 tattggcaga atatgaacct ttcagtagaa gaaatggttc agttagttaa agaaaaatat
    21841 gattccattg ataagaatgc acgacagccg atatcagaac gactgtatgg attgctcacg
    21901 gaactccaag aacaaaaaag aagccagcaa ttgctagagt tcaatgatgt actccaaaat
    21961 ctgaaaagcg ctttggagaa tgaggagatc cagcattata tcggccaaaa atatgattat
    22021 ctattcattg atgaatttca agatactaat ccacttcaat gggaaatcgt gaaacggatg
    22081 acaaaggaaa acaggatcaa gttgattgtt gttggagatg atgatcagag tatctatgga
    22141 ttcagaggat cagagccagc atacatcaag aactttgaaa aagaatatcc taccaaaact
    22201 cttttcttat taactaatta tcgttctcgg gcagctattg tccaaggagc aaatagattg
    22261 atcagctata ataaaaatga tcggatacca aaggcgatga ttccagcaca aaaagaagca
    22321 ggtgttatgg aagcctattt gttttctgat actaaagcag aaagtcagtg gctaatcaaa
    22381 caaattcaga catttattca aaaaaatggg aaatataagg aatcaatcat attataccga
    22441 tcagcttcac aaacgcagca gcttgtccaa tctctgctga aaacgaatat tccttttgta
    22501 ctggaagcag atagtccata tgaaggtatc tttggtatca agtctttcca acatttctat
    22561 caaaaaattg ttcattggca aacagcactg aatctgcaaa agaaaaatca ggcctaccag
    22621 caattgctcc ggcagctgat ggcagactgc tacctgaaaa aaagtgaatg cgatcaatac
    22681 ttttctccta aatatcaaaa tatagccgtt gcagattata ttttgtcgat aagccccaat
    22741 ttaaaatcga aatctgcaga aatccgagag tttgaggaag cgttgcgtaa aacagaccag
    22801 catcaagaaa tctattcgct tgttcaaagc tatcttcatc tgccacgtat agctaaggaa
    22861 atcgaccaga gtgatcaaga atggatacga gaagaagtaa agcagcatgc tactttcaga
    22921 agactccaag ccttatcgca agaaacccaa gcgacagcta aagaattaaa aacccgcttg
    22981 attgcttaca gacaaggaaa acttgatgca ctatgtatcc agagcattca caaaagtaaa
    23041 ggtcttagtt accgaaatgt ctttttgatt ggttgtaacg aaggaagttt gccttataac
    23101 ggagcgacag aaaagaaagt aatcgacttt aatgaaataa aagcagaacc agtgacgacg
    23161 atagaagaag aacgaagact attttatgtt gcgatgacta gagcaagaaa tcgtctgtat
    23221 ctatgtgtac ctcaaatgaa aaattcaaga aaattgaaag tatcgagatt tatcaaagaa
    23281 accgggagtc gtgtgaaaaa aggaaagtga tagaagagag ggtgtgacag aagtggtcag
    23341 tttcaagaaa gaagaaggaa tgtacgaaaa ttgcttctca aatttttgtg aaacttcag
//
LOCUS       DAXLPI010000037        22716 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXLPI010000037 DAXLPI010000000
VERSION     DAXLPI010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.037"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(445..975)
                     /locus_tag="ACF3T5_001228"
     CDS             complement(445..975)
                     /locus_tag="ACF3T5_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFD3631851.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1080..1631
                     /locus_tag="ACF3T5_001229"
     CDS             1080..1631
                     /locus_tag="ACF3T5_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFD3631852.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(1784..2257)
                     /locus_tag="ACF3T5_001230"
     CDS             complement(1784..2257)
                     /locus_tag="ACF3T5_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD3631853.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2395..3867
                     /locus_tag="ACF3T5_001231"
     CDS             2395..3867
                     /locus_tag="ACF3T5_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFD3631854.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLTKAGDNA"
     gene            3864..4574
                     /locus_tag="ACF3T5_001232"
     CDS             3864..4574
                     /locus_tag="ACF3T5_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFD3631855.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            4926..5210
                     /locus_tag="ACF3T5_001233"
     CDS             4926..5210
                     /locus_tag="ACF3T5_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFD3631856.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(5491..5910)
                     /locus_tag="ACF3T5_001234"
     CDS             complement(5491..5910)
                     /locus_tag="ACF3T5_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFD3631857.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGWFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(5912..6496)
                     /locus_tag="ACF3T5_001235"
     CDS             complement(5912..6496)
                     /locus_tag="ACF3T5_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFD3631858.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLNEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(6543..8018)
                     /locus_tag="ACF3T5_001236"
     CDS             complement(6543..8018)
                     /locus_tag="ACF3T5_001236"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFD3631859.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDVIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVKDWEKYLK"
     gene            8199..8510
                     /locus_tag="ACF3T5_001237"
     CDS             8199..8510
                     /locus_tag="ACF3T5_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631860.1"
                     /translation="MKKMEESFPKAAKVENIANILKVTFENGEVKYVKSHWTEEITDD
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(8625..10961)
                     /locus_tag="ACF3T5_001238"
     CDS             complement(8625..10961)
                     /locus_tag="ACF3T5_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFD3631861.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSD
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            11103..11771
                     /locus_tag="ACF3T5_001239"
     CDS             11103..11771
                     /locus_tag="ACF3T5_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD3631862.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     HIELIITDVMMPEMDGYDLVGSLRNARFDMPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSILTIGQTKLELDTYTVVQNQHSFILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            11774..12814
                     /locus_tag="ACF3T5_001240"
     CDS             11774..12814
                     /locus_tag="ACF3T5_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD3631863.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRVIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQVNGKIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAIIRLSK"
     gene            complement(13054..13935)
                     /locus_tag="ACF3T5_001241"
     CDS             complement(13054..13935)
                     /locus_tag="ACF3T5_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD3631864.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITDFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            14095..14823
                     /locus_tag="ACF3T5_001242"
     CDS             14095..14823
                     /locus_tag="ACF3T5_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFD3631865.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            14980..15297
                     /locus_tag="ACF3T5_001243"
     CDS             14980..15297
                     /locus_tag="ACF3T5_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFD3631866.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            15290..15898
                     /locus_tag="ACF3T5_001244"
     CDS             15290..15898
                     /locus_tag="ACF3T5_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFD3631867.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            15953..16744
                     /locus_tag="ACF3T5_001245"
     CDS             15953..16744
                     /locus_tag="ACF3T5_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFD3631868.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADYELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            16882..17370
                     /locus_tag="ACF3T5_001246"
     CDS             16882..17370
                     /locus_tag="ACF3T5_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFD3631869.1"
                     /translation="MKAEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            17421..18293
                     /locus_tag="ACF3T5_001247"
     CDS             17421..18293
                     /locus_tag="ACF3T5_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFD3631870.1"
                     /translation="MREKRAFYINGYAGIVGLIVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            18469..18831
                     /locus_tag="ACF3T5_001248"
     CDS             18469..18831
                     /locus_tag="ACF3T5_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFD3631871.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            18840..19592
                     /locus_tag="ACF3T5_001249"
     CDS             18840..19592
                     /locus_tag="ACF3T5_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3631872.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            19606..21408
                     /locus_tag="ACF3T5_001250"
     CDS             19606..21408
                     /locus_tag="ACF3T5_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3631873.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQTTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            21684..>22716
                     /locus_tag="ACF3T5_001251"
     CDS             21684..>22716
                     /locus_tag="ACF3T5_001251"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFD3631874.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSLDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINI"
BASE COUNT         7134 a         3983 c         4613 g         6986 t
ORIGIN      
        1 ctgggagcat tatttcattt tgaagtcact aatttaactg tgtgaatatg cagttgttac
       61 aatctttgat aaacggtgtt caaaagcttg catctacggt aataagccca accaaacaaa
      121 aaatatgagg agctattttt atttggctgt tcttattact tgatgcagaa cagctttttc
      181 acaacctctg attcacggtg ttccaaaagt agcttcttcg gaaataagcc gaaattttac
      241 aaaaatttga gaagcaattt ccgtaaattc cttcttattt cttgaagctg accacttctg
      301 tcacaacctc tttcttaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
      361 ggcaaaacat gaggaactat ttttccgttt ctttctctaa tactcaggtc agaacgctga
      421 tttcacaacc tctctttcac aaccttattt tactcgttta taattttcat cgaaatattt
      481 tccgctacgg atatcatctg gcattccatc gtatggttct tcgctaataa catcttcatt
      541 gtctatacct gcatcaccca tataagtgat agtagcaaat tcaggaacaa gtaattttgg
      601 ataagtttca tatttttcac gagctgcttc catttctgcg atatgttcgt tttggaaagt
      661 aacagtgatt tctggatgtt cttcaaccca tttagggaaa aagatcattc cgtgaagttt
      721 cttttcattt ggcataaagt ccaatgccac gtctgtccct gttggctctg tccaagtgat
      781 tttgaatacg ccgtccgtca atttaacgat atcagcttct tggtctttta cccaacggcc
      841 tttgaccatt ccgccatgga tacggtaatc aacagtatgg tcatttttgg cataccattc
      901 atattcccaa ccattatcat acgtatagat aaagtgagtt ccaataaaat cttccaattc
      961 tttaaattct ttcatgttta agcatctcct tataaattaa aacatgtaat aaactacatg
     1021 ttttcgatta ctcatttatg atagaatgga tttaaagatt tgtcaaggag ggaaaactga
     1081 tggcaagaaa aaatacactt cgatatattt tgttagggtt attaagtaag aagaagatga
     1141 caggatatga attgaatcaa tcgttcaaaa atgaaatagg agaattttgg caagcaaaac
     1201 atagtcagat ttatcctgaa cttgcaaaaa tggaagagca aggaatcatt cagcatcaag
     1261 tagagatcac aggtgaaaaa ttggaaaaga aagtttatga attaacagaa gaagggaagg
     1321 aacaattggc agagtggatt catacaccca ccaatgaact accggttaat cgagacgaat
     1381 ttgttctgaa attgtatttc gtcaaagacg tgaatgatcc ggcattagta gaaattattc
     1441 agcagcagcg tgaccttcat gaagaaaaac gacttcatct attagagcga aaaaaaacga
     1501 tctttcctac tgaaaaagaa aaacatgaaa attacggaca ctacctgatt ttagcgcatg
     1561 cgatcaatcg ggaaacagaa tatgcaaatt ggctaacaga tgtattagaa gaaagtaacc
     1621 gaaaaaaata aaaaaatatc cgaacagtga tggagaattc ctttctatat gaaagaaacg
     1681 cagatcaccg ttcggatatt cttatgttca aacaggcctt ttttcaagta tttatgtagc
     1741 atctacttat taaaaagctt ggtacttagg agattagtgg cgtttagagt tcttttaaca
     1801 gctgttcgta gtcagctttt tcatcgacag gaaggagctt gtaagttaat tcgtctgtct
     1861 cgttgtcatg aatgatgtac atcttagcat tctcttcttt gaagttaccg atttgtaaaa
     1921 tcgtatagcg ttcgtcgatc aattcgatat ctttgattgc ttttgaaaaa actttacgga
     1981 catggtgcat tttcttttgg cggatttctg ccttttctgc agcttttgac aaagtaaatc
     2041 cgtcatctaa atattttttg atcagttcta cttttatgac tgtaggtaaa cgatattttc
     2101 taggacaatt cgcatcagct tcgatcgaac gaatgaatcc cttttgttcc caatacctta
     2161 attgacgtgg agacacacct acgatatcac ttaattcact gatgccaaca accaattgat
     2221 cattttccaa caaacgttta agggaagccc caaccatttt tgtcactcct tttgtcctct
     2281 tgcttccctt aattatatcc gtattaactt atgtgtcaag taggttgaca aatagcgaat
     2341 ttaggactaa agtattctag tagtttcaga aaaaattcag aaagaaggag aaatatgagt
     2401 aacaaccaac ccgtagatat ttacggaaaa ccatataatc ggacactttt agttgttgtc
     2461 ttattaattg gtacattttg taccgttttg aatcagacct tattgacgac agcttttcct
     2521 acattgatga aagctttcga tatttcagcg tctgatgtgc aatggttaac gactggtttc
     2581 ttgctagtca acgggattat gatcccaata acagcatggc tgatcaataa attcagttca
     2641 agaaatttat atctttctgc catgacgatc tttttgatcg gaacgattac ttgctttaca
     2701 gcacctaatt ttagtacatt actaatcggt cgcttgatcc aagcttgcgg tgtgggagta
     2761 tcgatgccat tattgcaaaa tatcatgcta tctattttcc cgccagaaaa aagaggatca
     2821 gcaatgggga tgtcagggat cgttattgga cttgctcctg ctttaggccc tacattatct
     2881 ggttatatca ttgataacta ccattggcgt gacttgtttg gtatggtact gccaatcgtt
     2941 gttcttgtct tggttttagc attcttccta atgaagagcg ttattccatt gtcaaatcct
     3001 agcattgata ttttatccgc tattttatca acgattggat tcggtagttt gctttacgga
     3061 ttttctagtg taggagatga cggctgggga agcttgaaag taattggttt cttagttgtt
     3121 ggtgtgattt tcatcgcatt gttttcatgg cgtcaattac atttggaaca tccattcttg
     3181 gaacttcgcg tattcaaatc atctacattc acgattgccg ctgttttagc tggtgtaaca
     3241 aatatggcga tggtaggggc agaaatggtc gtaccattgt atattcaaaa tattcgtgga
     3301 gaatcagcat tccattctgg cttgatgttg ttgccgggtg cattgatcat gggggctatg
     3361 atgcctgtta cgggtgcgat tttcgataaa tatggcgcga aacgtttagc catcacaggg
     3421 atgttcattt tgacagccgc aactgcacca tttgctttct tgacaaaaac gactccagtt
     3481 ttatatatcg tgatccttta tgcgatccgt atgtttggga tctccatggt catgatgccg
     3541 gtaacgactt ctggtatgaa tgcattgcca aataacttga tcagtcacgg gacagcggta
     3601 aataatacat tccgtcaaat cgcaagctcg attggtacag ctattttgat cagtgtgttg
     3661 acgaatgtga caaaagcaaa tatcccaagc aaatctgtat tggataatac accattagct
     3721 tataaagatc aggcaatcaa tgccacactg tcaggttacc atgccgcgtt tatcgtagcg
     3781 atcatctttg ggatccttgg ctttattatt gcctttttat tgaagaaaaa agaacctgtt
     3841 ctaacgaaag cgggggataa tgcatgatta ttggagtttt gattgtcagc ttgcttgctt
     3901 ttgctttaac gaacgtgttc gctaaaaaaa catggcaaac ctttttatct ttgattttcg
     3961 gactaatctt cgtcgcttca ctaagtttga tcgtcgctaa tttgtccaat cactttggaa
     4021 tggaaaaagt aacggaaaca aaaacagaaa aaattgtttc cagtgctgat tcccaaggag
     4081 cagatatgct tttgtataaa gctttaggga atggaaaaga aaaagtgtat ctttaccgaa
     4141 caaatgaaaa acaagaaaaa ccaaaagcaa ctggtacgga taacgaaaca aataaagtag
     4201 aaaagacaga cggcgatgct gaaaaagtga cgaaaactac ttattgggaa tacaaaaacg
     4261 acatgtataa attctggttc aatattgctg ataacaatca cgaatatgac agccgagtga
     4321 atacattcaa aatcccagag acatgggtag aattaagtac ggaccaagca gccaagttag
     4381 ctgaattagt taagaaacag caaagtacga tggaaagtga agcaaaagct tatgttcaag
     4441 acggtatggt aaaagcaatg actgaaaatc caaaaatgag caaagcagag caagaacaac
     4501 gtacgaaagc cttagctgct gaattccaac aacaagcatt cgcaaaatta gtgaaagaag
     4561 caaaaggcga ataatatttt gaacaccgtg taggaggttg tgaaaaagct gctctgcatc
     4621 aagcagtaag aacaatcaga taaaaatagc actccatatt tttcatctga ttggggttag
     4681 tgccgtagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc tgccgtttaa
     4741 ctctgagtca caaggagcaa tttcaaaaaa cagcttctca tgtttgttga atattgtgac
     4801 ttgtgaccga ggagttggca ggagaacacc gtttattcga aaaaaggaac tgcgacaaga
     4861 cttttgtcac agttccttct tttttttgct atgctatact agatgtaaaa aataggagaa
     4921 ataaaatgag ggaaatttat tcttttgaat caaaaatcta tgcttccgaa gtcggcaagg
     4981 gcggggcata tattattttt ccgtatgata taagaaaaga attcggtgct ggaagagtta
     5041 aagtccaagc aacttttgac aatgtgccgt acgaaggcag tatcgtgaat atgggtgtga
     5101 aaaatccaga tggttcagtc tgctacatcc ttggactacg aaaagatatt cgtaaaaaca
     5161 tcggtaaaga aatcggtgat attgtagcag ttacagtgaa gcaaaaataa aagaggttgt
     5221 gacaaaagac gtgtcacaac ctcttgataa acggtgttca aaagccagtt tctcggtagt
     5281 aagtcaaatt tatgcaaaaa tgtggaaacc catgtttaca aaatttttct tactactcga
     5341 aacgaagcgg ctttatcaca acctcttgat aaacggcgtt caatcagctg tccctcggta
     5401 ttagctcaaa aacggcaaaa tatgagaagc tatttttcca tttttttctc tcatactcag
     5461 gtcataacgc tgatttcaca acctcttctt tcataatggt ttttcaccat ctttataata
     5521 aactggttct ttgatatgga aatctatttt ttccaaccaa tctaaaatct tgtaacctaa
     5581 ctcttctgac atcggaaaag aggcgttgat ttgtgcattg gagatatggt caacatggat
     5641 accggaagta atcgtacaac ttcccggaac ggcagttttg atgatcacag ctattttttc
     5701 ggctaagaca tcgtctttat ggaaccaacc atcgtggcta gggaaacgta cagtttgcat
     5761 ctctgtatca cgagaaaatg tagtgactgt tccgatatgt ggtgtatctc cgcctgttaa
     5821 agtgatcaat aagtctttac cgattacttt aacagcagct tggatcgtga aacctagttt
     5881 ggttacttca actatcgttt ctttttccat attaatcacc ctgccaacgt tttgaatgct
     5941 catttacaat tccaaattga tccaaaagtt tcattgtatg gtgattaaca agatcttgga
     6001 tcgtttgagg ctgattataa aaagcaggaa ttggtggtat gatttgcaca cctagtcgtg
     6061 ctaatttagt caagttttcc aagtgaatgg catttaatgg cgtttcacga ggaacaatga
     6121 ctaactttcg ttgctccttc attgttacat cagcagcacg tgcaataaga ttgtcgccta
     6181 aaccaacaga gatacctgcg actgttttca tacttgctgg tacgatgatc attccatcgg
     6241 tcaaaaatga tccgctggcg acagcagcac ctaaatcttt gtttgaataa tggtaatcaa
     6301 acaggctttc gaattcattt agtgataagt cggattccag ctttaagttc tgttttgccc
     6361 atggactgat gattccgtgt gtttctatca tcggatattc ttttagtttc ttcactaggt
     6421 tgatggcata aatggtgcct gaagcgccac ttacaccaat cacgattttt ttcttccgtt
     6481 gtgccaattg ttccattttc cgcactcctt tttatgatgt caaaacagtt gcagatcatt
     6541 cattatttca aatatttttc ccaatctttt acttcttgga attttgcacg tacaaaatgt
     6601 tctttcatat cgaaaggaac tgtaccgtca aagatagttt ttgcactcat cccacggaaa
     6661 cgaatagagc caggattata ttctggtaat tctgatgggt ccaatggatg gtttctcatt
     6721 cctgggatca caatgacgtc tttatctcct tggaaacgag tgttgaaagt ccaaactaca
     6781 tcgttcatgt cgaaaatatc aacatcttca tcgactaaaa tgactgtctt caattctttg
     6841 aaggctgata aagcaagaat agctgcttga cgctgaatac cttcatccgc ttcgttttct
     6901 ttacggattt gcatgatggt catcaattta ccaccacctg caggaggatt gtagacattt
     6961 actacttttc ctggaatagc tttatcgacc aaggcaagaa tacttgcttc tgtaggaata
     7021 ccggccatag atacgtgttc ttcactggga ccaatcgttg tttgcatgat tggattgtct
     7081 tttcggtgag taactgcttt gactttcact acttgtacag ctggattagc atccccagta
     7141 tagccaggaa attctggcat tgcttttcct gagttcgtgt tgatatcttc ttgcattgtt
     7201 tcatttggca tgatttctgc ttcaatgatg aattcagcac gagcaatacc cacttcatcg
     7261 actgctatac catcaacgat ttgaataggt tcgttacgca aagctcctgc acaccatagt
     7321 tcattgtagc caagtggagt ggtaggtggt tcgaaagtcg tgccaatggc aattgctgga
     7381 tctaaaccaa tattgatagt gataggcatt ggtttattca aggcttcgaa ttgtttttgg
     7441 aagtgaccaa tgtggcgtcc gccaggcatg atgtacatac cgatcgtatc tttatcttct
     7501 agaaccatac gatggatcgt tacatcgctc attgtttttt ctggatctga tcctaagact
     7561 acacccattg tgatgtatgg accagcatct tcaggagtat ttgtaggtgc aggcaaaatg
     7621 ttgcgaatat cgaagtctgc atcggtagct agatgaacga cttcttgtgc tggagcgtct
     7681 gctttgtcta ctttcacagg tttgattgga ttttctacag cgtctttcaa gaaacgacca
     7741 agtgttcgat aatcatggtg caaaatttca cctacacgtt tacgactagc cattgttccg
     7801 atgttcacac gtgtgccggg gaaacctttg atattgttga aagtcatagc tggtccttct
     7861 tgagtcggac gttttactgt accgccagca ccgatataac ggtaaacacc agataattct
     7921 gcttcaggat cgatttcaat atctgtttcg tgatattgac ctggaagtgt ttttagttct
     7981 tcgatgactt ttcttaaatc atacggttga tctgtcatga gagattcctc cttagttgtc
     8041 tttacctatc tatagtaaaa ggtgaaaaag aggaaaacaa ttcaacttgt tcggtaaagt
     8101 attctacaat ggaatggatg cgtttttttg ataatagaat aaactatgat atacttatca
     8161 ataaaagaaa gcgcatactt agtgttggag gaaggaaagt gaaaaaaatg gaggagtcat
     8221 ttcctaaagc agcaaaagtg gaaaatatag cgaatatttt gaaagttaca tttgaaaacg
     8281 gagaagtaaa atacgtgaaa tctcattgga cagaagagat aaccgatgac ttgcaatttg
     8341 gaaaaaaagg aagaggaaaa agaaaaaatc ttctcgcact ttctcgtaat atgtggattg
     8401 ggactgaagt aacgattgaa gcagatggaa cagtattcat aaatggaaaa gacaggtata
     8461 cgccagaaga gttgtggtac aaaggaaaga aaagtatacc agaattataa aaaataatta
     8521 aaaaaaacag cgcgaaacaa ggaacatatt acttcctcgt ttcttgctgt tttctactat
     8581 ttcagcgctc ttatgactca aatctaaatg tggttgtcac aaccttattc tgcacgcaaa
     8641 gcgatagcgg cgtctttttt cgctgccatt cgtgcaggaa gatgaccgcc aagcatcgtc
     8701 aatatagtag agacgatcac tagaataatc gcatggacag gatttagttg agcaacattt
     8761 tttagatccg tcatgttgta taagatgcta ttgataggga atgtagctag ccatgcaata
     8821 aacacaccta atatcccaga agaaatacct aaaatacacg tttctgcgtc aaatacgcga
     8881 gtgatgtctt ttttacgtgc acctaaagct tttagtacac cgatttcttt tgtccgttca
     8941 atcacagatg tataagtaat gatactgatc atgatcatac ttgttactaa agagatccct
     9001 gcaaaagcaa taaggacata agtgatagca tccatcaatc cgccagtcag ttcagtcatc
     9061 gttccagcta aatcagtata aatgatttta tcttcatctg atttgccttt attgtaatca
     9121 tctagataat ccaagatctt ttctttgtct tcaaaattgt tcggataaat catgatactt
     9181 gaaggcaagc tgtctccacc taaataacta agcaatgttt gtttggcatt ttcgtcaact
     9241 ttttcagtag ttaagacatt tacatcactg tctctttgag ctttgacgat ttctgattct
     9301 ttgttttcgt tgacgatttg agtcgttaat tgatcgctat aagcgattcc tggagataag
     9361 agattcattg tagaagaaga tttcacgcgt aggatccccg agatcttcaa ttcatcagaa
     9421 gcattctgat acatcgcatc ataatctgta ttagggataa aattcccagt tggaagtttt
     9481 gtatagtaag tattgttgtt tactaatttg aatgttgtac caacgatatc atcaaaatca
     9541 agtttttcgt cttctttcac atcaaatcca agattcttca atgcgttgat attagtatta
     9601 ttattgccgt ctacgatcag cacgacatct gttgctgagg ctggataact tccggcaagt
     9661 aaagaataat tgtcttttaa aaagttttct ttgctagtgt caagctgtgt agggaaactt
     9721 gacactccca cacctgtcat tgctgacatc gtactagata aagaaagaga ttccgtatca
     9781 ggattttgat tcgagaagct gacaggctgt actttgccat ttacatcacg taaaagattg
     9841 attcctgtag tacgggtaaa tccgatatta ttacttaagt taggatcgat gtctgttacg
     9901 taatcgacgt atgtttgatc aatcttattc gtgtgctgtg ctttatcttc ttcgctgact
     9961 tttgcagtaa tcgttttgct gtctggataa tccgctttgc ttgcacccaa tccggcatcg
    10021 tcacgagtct ggttagtcgt taccttagaa atagtaatcg gaaatttcgc catcgtttca
    10081 gcttgcgtgt tatcgatttg tttttggaat ccagaagaaa gggacagaac gatcgcgatt
    10141 ccgatgatcc caatactgga agcaaaggag gtcaagaaag tacgtccttt ttttgttcgg
    10201 atattgttga aggaaagctt caacgctgtc caaaaactca tctttgttct gcgtaagtta
    10261 aattgatcat cttttggccg ttcgatgtga ggatgggaat ctgttaagat cttaccatca
    10321 gaaaattcaa tgatccgatc tgcgtattga tgggccagtt caggattatg tgtgaccatg
    10381 atcactaatt tttctttcga taactcttgg atcaattcca tgatctggat acttgtttct
    10441 gtatctaacg ctccggttgg ttcatcgcac aaaaggatat ctgggtcatt ggctagtgct
    10501 cgagcgatgg ccactcgctg catttgtcca ccagataact gatttggctt tttatgcata
    10561 tgatctgtca agccgacccg gtgcaatgca tcttctgccc gttttcgttt ttcgtcttta
    10621 gaaacaccac ttaatgtcat cccaagctca acgttttcaa taatgcctag atgaccaatc
    10681 aagttgtaac tttgaaaaat gaaaccgata gagttgtttc ggtaggcatc ccagtcactg
    10741 tccttaaagt ctttagtaga ctttccgttg attaccatat caccggaatc ataattatct
    10801 agtccaccaa tcacattcag catagtcgtt ttaccagaac cactaggacc taaaatcgct
    10861 acgaattctt tttgtctgaa agcgacagac acaccatcca atgctttggt ggtggtttcg
    10921 ccgactttat aatatttttt tatatctttt aattgaagca taatcaccct tctcccgtat
    10981 tgaaatattc ggtatactaa taagtatatc agaacaatga tcgtagtata cgaaataaat
    11041 gttaactgaa gatgaactac aagaaaaaat ctagaaattt ttcaaaaaac agagggagat
    11101 caatgaatcg tattttagta gtagaagatg atccgcaatt acagttgctt taccgttctg
    11161 tgttggaacg agcgggcttt tcagtcatca ccgcactgaa tggagaggat gcgctgaaaa
    11221 aactagaagc gacacatata gaattgatca ttacagatgt catgatgcct gaaatggatg
    11281 gctatgactt ggtaggatca ttacgtaacg ccagattcga catgccagtt ctgatgatta
    11341 cagcaaaggc tgatttcgag gacaagaaac gtggattcaa attaggtgca gatgattata
    11401 tgacaaaacc catcgatgtc aatgaaatga tcctgcgtgt ggaagcattg cttcgacgtt
    11461 caaaaatcaa ttacagcagt atcttgacga ttgggcaaac aaaattagag ctggatactt
    11521 atacagtggt acaaaatcag cattctttta ttctgcctca aaaagaattt ctgcttctgt
    11581 ataaattact gtcttatcca aacaagattt tcactcgcca gcagttgatg gacgatattt
    11641 ggggattaga cacgaataca gaagaacgaa cgatcgatgt acatatcaaa cgtttaagga
    11701 cccgttttga aaagacagaa gattttcaaa tcatcacagt acggggacta ggctataagg
    11761 cggttattta aagatgaaaa agaagctatt ttcacaatta tggatctatt ttgctagtat
    11821 cgtatttatt tcgatcttag taacactgct gtgttttctt ggttttattt tcttattgtc
    11881 tcgacattct atctcgcctg ctgaatcggc tggaaaacca acgttatacc cgttattcgt
    11941 ctttgcgggg ttcagtatgg tcgtgggtac aggaatatcg atttttgttg ggcggcgtat
    12001 cctgcatcca atcagtgcgc tagggacaaa tatgagtctg gtagcaacgg gagatttttc
    12061 gattcgaatg gatgaacagc agaaagtagc agaagtacag caactgtata aagatttcaa
    12121 cgttatggtc caagaactca atagtatcga gacactgagg aatgactttg tgtccagtgt
    12181 atcccatgaa ttcaaaacac cactagcaac aatccaagga tatgttcaat tgttacaagc
    12241 cccaaatctt tctgacgaag aacgccaaat atttttatat cgggtcatcg aaagtatcac
    12301 gcagctttca caattaacag agaacatttt aaaattaaac aaactggaaa accagcgtat
    12361 ccaactggaa aagaaagagt atcgattaga tgaacaaatc agagaagtaa tcgttttttt
    12421 gcagcctaaa tgggaaaaag aacagctaga attggatatc gaacttgctg cagtaaacta
    12481 tacaggtaat gaagagtttt tgtaccaagt gtggctgaat atcatggata acgccataaa
    12541 gtacaatcaa gtcaatggaa agattcatat caaattattt gagacagcca cagagatcgt
    12601 actggaagtg accgattcag gtgtaggaat gaatgaagag acccgagatc gaatgtttga
    12661 aaaattttat caaggagata caagccgtca gatttctggg aatggactag ggctttcact
    12721 ggtcaaaaaa attctagaac tacatgatgg acgtatcgat tatagcagta tcgaaggagt
    12781 agggacgaca gcaataatac gtttaagcaa ataataggaa aaaagaacaa aatacctaaa
    12841 aattctagtg aaggttttta gggattttta gacgtttttt tgtgattagc gaattgctct
    12901 taatgttctt ccggcataaa atcgctgatc ttctattgaa atacgagaaa cgttagatgt
    12961 gtcattgaat caatggcacg atgtaagaaa gagagcgaaa gtcatgcgtc gctctctttt
    13021 ttggggtgga tagatggaca gaaaatgtag ggcttagttt tcttcttgat ttaaatactc
    13081 ccaaaatgta tctgaagcca aagaatgctc accgacttta cgagtaaatg caagataact
    13141 ctctgttgtt ggagaaatag ggagaatcac agtattttct tttaatagat tcttgactgt
    13201 tttttccata acaatagcaa tccccatgtc atttgcaatc atattgatga tcaagtcgat
    13261 tcttgctcct ttataagaga tttttggttc aaatccagct tcgcgacaca gatctagtac
    13321 aggttgatac aagttcgtag tggggccaag tagcaaaaag ttctcctttt tcagttcgct
    13381 tagatccaat ttctttttac tagctaaagg atgattttta ggtagaactg ctacaaaatc
    13441 gtctgtttca gtaggcaacc actcaatcga actcggcggg gcctggaaac ttctagcaaa
    13501 gtggatcgtg tcactatgtt cattggcgtc attgtaagtt aattcgacac tttccacttc
    13561 tttcaactgg actgtaaatt caggatggcg tttcaaaaag tcggtgattt tggaaaaacc
    13621 tttataattg atcattgtcg gtatcgttaa gatatttaat gaaagattgt tgtctttcgt
    13681 ataggtttca agcacttctt gcatagcatg gtattgttct acgacatttt ttacatatgg
    13741 gagcaataag cttccagctt cggtcaattc gatttttcta tgggcgcgtt cgaataattt
    13801 gacgtctaat tctgtttcta acgaaagaat ctgtttagaa atatttcctt gggtggtgaa
    13861 ttgtcgatca gcagttttgg tgaaattcat tgtttcggct aaatcaagaa aaacttcgaa
    13921 tttatggata tccatcatat tcctcctact tattccaata aagaatactt taacacaaaa
    13981 aatgaattgg tgtaatcttc tctgtctgtt acaatctttc ttgcagcgaa agcaataatt
    14041 acagaaattt ttctgatttt tttcgcttcg tagaaaaaga gaggagtttt tttgatgcca
    14101 actggagttt tgatcaatgt aggttccgtt ttgcttggcg gactaattgg cggattagta
    14161 ggaaataaat taagtgaaca ttttaaagca cagctgacga tggtttttgg tgtgtgttcg
    14221 atggggatgg gaatctattc cattgcgccg atgaaaaata tgccggctgt tatttttgct
    14281 ttagtgatag gaacagcgat tggtctgatt gttcatttag gtaatgggat aaataagggg
    14341 gctgcgctga tgcaagtacc tatttctaaa atcttcccta gtgaaaagct tggtatgacc
    14401 cacgatgaat ttatcagtac attagtgacg gtcattgttc tattttgtgc aagtggtacg
    14461 gggatctatg gaagtttaga ttctggaatg acaggagaca gtacgatttt gatttctaaa
    14521 tctgtccttg attttttcac agcagctatt tttgcgtgta atttagggta tgtcgtttct
    14581 gtgatagcaa ttccccaatt cattattttt tatattttgt tcttattggc aaaatttatt
    14641 tatccattga ctacaccaga catgatctta gattttaaag cttgcggcgg attcttgatg
    14701 gtcgctaccg gttttagaat gatcaacgtg aaaatgttcc cagtagctga catgatacct
    14761 gcaatgatcg ttatcatgcc actgagctgg atgtggacaa attggattat gccactgtta
    14821 taataaaaat caaagagaag ccattttttt gctgactaaa aaggatcaaa tcttttagtc
    14881 agtttttttg tatttgacaa actgattttg aaactatatt atatgttgta taatatagat
    14941 tgaaataaaa tattacactt gagaatagaa ggtgacgtaa tgcacatcca gattccaact
    15001 gttcttcttg atggtactgt gttagcagca ttaagcaagg aagatatgta tgggtatgcc
    15061 ttaacaaaaa tcgtacaaga aagtctctct gtcagtgaat ctacactata tccggttttg
    15121 agaagattga aaaaagaagg atatcttgag acctatgatg aaccttttga aggcagaaac
    15181 cgtcgatatt actcgttgac accaatggga agtcgtcatt tagaagaaat tattgatgaa
    15241 tggattgttt ttcgagattc tttagaccgg ttattggagg agagcacaaa tgaatgaata
    15301 tttgaatgaa gttgtggatc atcttcaaga aattcctgaa gacgatcgtt ttgatttgat
    15361 ccagtattat gaggaatatt ttttagattc tggaaaaaca ttagatcaga tcattgatga
    15421 atatggtaca ccaaagcagt ttgccttaaa attaaaaatc agttactttt cagaagaaga
    15481 acaagcagaa aacgagttag tgaaaaatcc aaaaagacaa attcgcctga tttggttaat
    15541 cattatcggt ttatttgctt ctcctgtcct cattccacta gcactggggt ttatattggt
    15601 tattggggga ctattggttg ctttactagc gctcattttt agtatctatg ttttatgtat
    15661 cagtatctta ggtgttggcc tattcatgat catatcagga ataagtgtgt ttggacaatc
    15721 gattctgagt ggattattct ttatcggtgt cggtctgttc gccacaggaa gcgcagtatt
    15781 tatttcgcca ctgttgctga aaggaacaaa agggcttttt tatgtcatga tgaactttgt
    15841 taaatggatg ggacgacgtt ttattacaaa aagagatctg cattcagcgg gcgtttgaag
    15901 gaggacacat aaattgagat tgaaacattt tttgattacc ggtattggtc tgatgcttac
    15961 tggtggcctc ctagcaggtg caacttatac attaggcgct caaaagagtc tgacttggga
    16021 taatggtcct cggttgatcg atatggttac tgagacaaaa aaaatcaagg atgcaaacaa
    16081 aatcgtcatc catgcagaaa atcaaacagt ttcggtcata cgcggattgg attttgagat
    16141 aaaaacgact tatgataaga cagaaaagcc agaaataaag gagaaaaatg gcgtgttgac
    16201 tgtgaatgca gtcaaaaaat caacaggtgc aatcattagt ttagaagaca caagtgcaca
    16261 aatcactatt gttgtccctg cagattatga attatctgaa ttgacaatcg aagggaataa
    16321 tagtgcaatt gatatcaacg aagtatcaat caaaaaagtg aatactaaga cacatgatgg
    16381 ctgggtatcg ttcaatgaat ttacaggagg aacaataact gcgaaagatg aattaggctc
    16441 acttcgacta gatgaggcca atgtggataa cttgatcgtc acaggtgaaa acagcaactt
    16501 ccatctagaa gacagtttga tcagcaaaga aggaaaagtc atcctaaacg gtggtaattt
    16561 atctttgcat gagatggaaa acagtggcta tactatccaa acgtctggtg aatcttatat
    16621 tgaaaaagac taccagccaa taacaaatac tttgttagaa caaggacaga aaaagctaaa
    16681 gatcactgct aaagacacat cgatcaatat tacgacggat caagaggaca atgaaacaga
    16741 ttgatcctgt actcaactag atattttaag aaaaaacagc aaagatcctt ccaatctttg
    16801 ctgtttttca tcgcttaacg cacataacta tagtaaaata agaaagaaaa tatctataaa
    16861 gaaaacaggg tgaaaaagta tatgaaggca gaagaaggtc aaagagaaaa gttaaagaca
    16921 gaaatggaaa gattacatac ttatatcact caactaagcc aaaccttcta tgatccagac
    16981 aaagaaaaag tgatggttaa ttacccgaac aattcagaag gtcgccaatt agagcaagtg
    17041 taccatgaag ttttcaaaca tttattgacg gtgaagaaag agctggatta ttattcttta
    17101 ccgattatcg atacaggaat attgaagtat gatgggaaga aggaacgatt tatctttaaa
    17161 tctgttcggg aagatttgcc tctttcagca gggatggatc tagaggtctt ggtagaagac
    17221 tattttacag aagaaaagca ctgggttcga acaaaattgg attatttacc gcaggcagcc
    17281 ggagggacac aggcaagcgg ttggtacatc actgaagaca aagagttaga actagaaggt
    17341 gtaatggcgc gtattcgaaa aaaaaattaa aaaagtaaca ttagatattt attgaaggat
    17401 aaaggagcgc aggaagtagc atgagagaaa aaagagcatt ttatatcaat ggttatgcag
    17461 ggattgtcgg attgatcgta ttagctttag ttggattgtt tttcttctat ttaggcatgt
    17521 ggcaagcgaa agtttgggca ctttttttaa gtatttttct ttggttgatc acgttattgt
    17581 tgttaagttc ggcaactgta gtcagtccta atcaagcaaa agtaattttg ttttttggac
    17641 aatatttagg tacgattcga gaaaatggct ttttccttac gatacctttg gcacaaaaaa
    17701 tgactgtttc gttgaaagtt cggaatttca atagttctgt tttaaaagtc aatgatttgg
    17761 atggaaaccc catcgaaatc tcagcagtag tagtctttaa agtcgttgat acagcaaagg
    17821 cattattcga tgtagcgtat tatcaagatt ttgtagaaat ccaaagtgag acagccattc
    17881 ggcatatcgc tagccaatac ccgtatgata cattcaatga gaatgatttg acgcttcgag
    17941 gaaacacgac ggcagtgtct gacgagttgc aaaaagaact gcaagagcgt ttagcagtag
    18001 caggtgtcga agtaatcgaa acacgcttga accatttagc ctatgcaaca gaaatcgcta
    18061 gtgccatgct ccaacgtcaa caagcaaaag ccattttgtc tgctcgacaa accattgttg
    18121 aaggagctgt cacaatcact caaatggcct tagagcaaat cgaagatgga caagaaatca
    18181 atttcaccga tgatcggaaa gtccaattga tcaataattt acttgtctca atcattacag
    18241 acaaaggaac gcaacctgtt atcaatacag gcgacatgaa agaccgtaag tgacatactt
    18301 tttgtaagat aggaagaagg agtttaggac ggattattgt cttgaactct ttttttagtt
    18361 gttttaaaga aaatgacaaa attgaaagaa gaatgatagt tgattcgaag gcagaattgt
    18421 tcgctttttt ctataatcag tatataaagt aagagagaag gggaaaaagt gaaaaaactt
    18481 ctgatcggtc tacttgtttt catcgttgtg gtacttgcag gggtgaaaat cgcagattac
    18541 gtagttatgg gtggagaaga atactatgta caaattacga cagatggtac aaaaacagtt
    18601 tcaaaatcag actctggaca agaaacagtc aactatagat atatcctgcc tggctatacg
    18661 aaagagggta aggaaaaaga actggacttc aagggacaat tgactcgacc gcttcgtaaa
    18721 ggagcatatt taaaaatcac ctggaacaaa aataaaggtg tcacttcgta tgaagaagtc
    18781 gaaaaaaaag acgtgccgaa aacagcactg gaaaaactaa ttaaagggta gggaaagaaa
    18841 tgaacaaaat agtcgaagta cagaatgtaa caaaagtata cggaaaaaac aatgagaaaa
    18901 agacgcaagc attaagcggc atcagcttcg atgtagaaaa aggcgaattt attgggatca
    18961 tgggcgcttc tggttctggt aaatcgacac ttttgaatat tttatccaca ttagataaac
    19021 caacagatgg gcatatccga atcaatcaaa aagatgtgac cacattaaaa ggaaatcaac
    19081 tagcagattt tcgtgcgaat gagattggct ttattttcca agactttaat ttattagaaa
    19141 gcttgacagc acaagaaaat atcgctgttc ctctttcttt acaaggcgtc agaccaaaag
    19201 aaatcaagca acgtgtccaa aaaattgcag aacgtttatc catctctcat attttggaaa
    19261 gctatccttc tgagatttcc ggtggacaaa aacaacgggt cgccgccgca cgtgcattga
    19321 tcacacaacc aacgattcta ttaggggatg agcctacagg tgcgctggat tccaaaagtg
    19381 cccgggatct tttagatacg atggacgaat tgaatacaaa agatcatgta tcgattcttc
    19441 ttgtgacgca tgatccgttt tctgcaagtt attgccaacg tattttattc atcaaggatg
    19501 gcggtatcca tcaagaagtg aaacgtggag atcaaagccg tgattccttc tatcgagaaa
    19561 tactatctat tcttggaaac ttggaacagt agggggagat tgactatgct ttggaaatta
    19621 tcacttactg ggatcaagag ccgtttaaga gactacttgg tgttattctc aggattagtt
    19681 atggcatctg ctatttttta catgtttgaa tcaatggcaa gcaacgaagc ttttttgaaa
    19741 agtaatacga tcgttgcggc tacagttatc attttccgat tcggttctgt tttactaggg
    19801 atcatcacat tcgtttatat tttatatgca aattcctttt tgatgacgat gcggcaaaaa
    19861 gactatgcaa tgtttatgat gctaggagca aaaggcagaa aaatcgcaca gatgattttt
    19921 tctgaaacat tcttagtagg gatcgcagct actttagtgg gttcagctgt cggtgtagga
    19981 ttgacaagta tcgtccaccg attattagtg gatcagttag atctcaatat ttcgcatttt
    20041 acacctttta gtattcacgg gttgttgatc actgttttgt tttttgctgt tttgttttta
    20101 ttagctgctg ttgtcaatgc tttttcgatt gtcaaaaaac cgattttagc attgattcgt
    20161 gcggatcaga caccaacaag aatcaagcag cataaattct tgtttttcct tgaagtagta
    20221 ctgggaattg cttttctagg ctgcggttac tatatgatga aacacgtggg gacctatcaa
    20281 gtctttggta taggagtagc cttagttacg atcgtattag gaacttattt cattttccat
    20341 tctgtgatca ttttcttttt atcattacta aagaaatcag aacagatttc attgaaaaaa
    20401 ttgaacaatt tcacattgtc acagttaaac tttcgtattc gtgaatatac acaaatgctc
    20461 tcaatggtcg ctatgctgtt tgccttggca ctgggcgctt tgacggtagg attaggtttt
    20521 aaaaatgaaa ttgcagactt gacagaaaac aattctggat atgatctagt gatgaataat
    20581 cctcgggcag aagatcagca aaaaatcaaa gaactttcac caacactttc tgcttcttat
    20641 actcaaaaag aagatgcggg aacgatttac tataatgcaa gtgaatttgc ggctcaacca
    20701 ttgatcatga taaaacatga agatggaaca ccgccgaaga tcacgaaaga aaaagttcct
    20761 gctgaaaaaa tgaatgaact gactgtacaa gaggaacttc gaagctatga attgccggaa
    20821 cagcaagaaa aagaaatcaa ggtagtcagt caatcagaat ttgatcaact gaacttgcca
    20881 caaaccactt tacagctagt tcaggtaaaa gacttccaag caaacctgaa accaatcgaa
    20941 acacttgttg ctcaaaataa aatgaacaat ccatctttgc aaaaagtaga gtacagcaac
    21001 cagaaatatg aaatgtatga agtgtataat ggtctattct ctggttttga atttatggga
    21061 ttcttcctag gtttagcttt cttgaccatg cttgctagct gtttgatgtt caagatcttg
    21121 tcaggaagca agagtgacat tgctcgttat gaaatgcttg aaaagatagg aacacgacgc
    21181 gggttgctta aacaatcgat ccgtagagaa gtaggcgtgt tattcttagc accaggaatc
    21241 ttaggtgtag tccatgtctt attcggatta caaatgtttg gtctattcat gcaaaatcca
    21301 tacaaagata tttgggttcc gtttacgatc ttcttcgtat tgtatttcat ctactatgta
    21361 ctgacgacat ggttatatac tggaatcgta ttgaaaaaga gattgtgaaa aagctgtcct
    21421 gcatcaagta ataagaacag ccaagcaaaa atagctcctc atatttttcg tttgattggg
    21481 cttattaccg cagatgcaag cttttgaaca ccgtttgttc ggaaaaagag gttgtgacaa
    21541 tacttttgtc acaacctttt ttcgcggtct tttattagaa tatagtaaat tttttctcgt
    21601 tactcacgga ataagctgtt aagaaaacgg tttctatgta tactggaatc agtgcaaatg
    21661 aaagaaaaga gggtgcaagg aacatggaaa tcaatgaaca aaattttggc gaaaactatc
    21721 acttgattac tatcacgaat caaaatggga catctttgtc tgtcagtgat cttggggcac
    21781 gggttgttca ttttcaaaca ataatcgata atgaaaaacg agagttgatc ttaggttttg
    21841 atgaagcaaa agaatatcta gaaaaagacc cttatatcgg tgcttcaatc ggacgtatgg
    21901 ctggacggat cacgaatggc acattcaaat tagatgatga aacgttcgaa gtaaaaacag
    21961 acccggacac tggtcattgt ttacatggtg gtgcacctag ctttgaagcc aagcagtggc
    22021 attatacagt catcaatggt gaaaacgaag catcggttat tttctatcag actagcccag
    22081 acatggaaaa cggatttcct ggaactttag atgtagaagt acggtatacg ctgacgaacg
    22141 atgatacatg gcgagtgacg gtccaaggga tcagtgaccg caaaacagta ttcaatccta
    22201 caaatcatgt ctatttcaat ttaaccggtg atccagcaca gtcaatcgac gaacatatac
    22261 tttggttgaa tagccgatta tttgctacgc tcaataaaga tacaacgcct acaggagaag
    22321 tagtttcagt aagtggcaca gcatttgact tccaacgtcc taaaaaatta ggtgaggtct
    22381 ttgccagtga ttttcgccaa tcaatgatag tgggcggtat cgatcatcca tttttgttga
    22441 aaaaacaatc tggtttacta aatgctgcgg aattgacaag cctagatgaa aaagtatcga
    22501 tccaagtaaa aacagatgct tcaggaatcg tgatttttac agcaaatttt ggagaagatg
    22561 gaccggaaat gcgcgggaat aaattagtaa atcatggcgg tatcacattt gagactcaag
    22621 aactgccagg agcggagcga cttgaaagtt ttggagatgt cactctggaa cctaatcaag
    22681 tacgcgagtt tgtaacggaa tacaaaataa atataa
//
LOCUS       DAXLPI010000038        22394 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXLPI010000038 DAXLPI010000000
VERSION     DAXLPI010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.038"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            82..1023
                     /locus_tag="ACF3T5_001252"
     CDS             82..1023
                     /locus_tag="ACF3T5_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFD3631875.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1092..1823
                     /locus_tag="ACF3T5_001253"
     CDS             1092..1823
                     /locus_tag="ACF3T5_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFD3631876.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            1967..4147
                     /locus_tag="ACF3T5_001254"
     CDS             1967..4147
                     /locus_tag="ACF3T5_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFD3631877.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4167..4649
                     /locus_tag="ACF3T5_001255"
     CDS             4167..4649
                     /locus_tag="ACF3T5_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFD3631878.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            4665..5426
                     /locus_tag="ACF3T5_001256"
     CDS             4665..5426
                     /locus_tag="ACF3T5_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFD3631879.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            5557..6891
                     /gene="murC"
                     /locus_tag="ACF3T5_001257"
     CDS             5557..6891
                     /gene="murC"
                     /locus_tag="ACF3T5_001257"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFD3631880.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7198..7683
                     /locus_tag="ACF3T5_001258"
     CDS             7198..7683
                     /locus_tag="ACF3T5_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFD3631881.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            7676..8359
                     /locus_tag="ACF3T5_001259"
     CDS             7676..8359
                     /locus_tag="ACF3T5_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFD3631882.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8485..11130
                     /gene="polA"
                     /locus_tag="ACF3T5_001260"
     CDS             8485..11130
                     /gene="polA"
                     /locus_tag="ACF3T5_001260"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFD3631883.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11175..12011
                     /gene="mutM"
                     /locus_tag="ACF3T5_001261"
     CDS             11175..12011
                     /gene="mutM"
                     /locus_tag="ACF3T5_001261"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFD3631884.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            11975..12604
                     /gene="coaE"
                     /locus_tag="ACF3T5_001262"
     CDS             11975..12604
                     /gene="coaE"
                     /locus_tag="ACF3T5_001262"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFD3631885.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            12656..12988
                     /locus_tag="ACF3T5_001263"
     CDS             12656..12988
                     /locus_tag="ACF3T5_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFD3631886.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13300..13791
                     /gene="nrdR"
                     /locus_tag="ACF3T5_001264"
     CDS             13300..13791
                     /gene="nrdR"
                     /locus_tag="ACF3T5_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFD3631887.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            13814..15187
                     /locus_tag="ACF3T5_001265"
     CDS             13814..15187
                     /locus_tag="ACF3T5_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFD3631888.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15187..16113
                     /gene="dnaI"
                     /locus_tag="ACF3T5_001266"
     CDS             15187..16113
                     /gene="dnaI"
                     /locus_tag="ACF3T5_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFD3631889.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16242..16682
                     /locus_tag="ACF3T5_001267"
     CDS             16242..16682
                     /locus_tag="ACF3T5_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFD3631890.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            16826..17527
                     /locus_tag="ACF3T5_001268"
     CDS             16826..17527
                     /locus_tag="ACF3T5_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFD3631891.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17524..18645
                     /locus_tag="ACF3T5_001269"
     CDS             17524..18645
                     /locus_tag="ACF3T5_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFD3631892.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            18660..19892
                     /gene="pepT"
                     /locus_tag="ACF3T5_001270"
     CDS             18660..19892
                     /gene="pepT"
                     /locus_tag="ACF3T5_001270"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFD3631893.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20024..20932
                     /locus_tag="ACF3T5_001271"
     CDS             20024..20932
                     /locus_tag="ACF3T5_001271"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFD3631894.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            20967..21239
                     /locus_tag="ACF3T5_001272"
     CDS             20967..21239
                     /locus_tag="ACF3T5_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFD3631895.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21250..22296
                     /locus_tag="ACF3T5_001273"
     CDS             21250..22296
                     /locus_tag="ACF3T5_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFD3631896.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7546 a         3916 c         4916 g         6016 t
ORIGIN      
        1 ttatacacaa gcaaataaat cgtcaaatgc gaacgccttc tttatactgt ttaggaacga
       61 aaagaaggaa ggaagaaata catggattat caagtactgc tttattacaa ttatacaaaa
      121 atcgaagaac cagaggaatt tgccaaaaat catttagcct tttgcaaatc tttggatctt
      181 aaaggtcgta ttctagtagc tgaagaagga ataaatggta ctgtgtctgg aaccgttgaa
      241 aacacgactg cctatatgaa agcaatgaaa gctgatcctc gatttgctga tacgatgttc
      301 aagatcgatc caagcgaagg acatgccttt aagaagatgt ttgtaagata ccgtccagag
      361 cttgtaagtt taaagctaga agaggacatt gatccaaacc aagtgacagg tgcctatctt
      421 tcaccgcaag aatttaaaga agctatttta gatgaacaga cagtggtgat tgatgcacgg
      481 aatgattatg aatatgactt gggacatttt cgaggggctg tacgcccaga tatacgcagt
      541 tttcgagagc ttccagactg gatccgcgag cacaaagaag aatttatgga aaaacgggta
      601 gtgacctact gtactggtgg cattcgttgc gagaagtttt ctggttggct tgtccgtgaa
      661 ggattcaaag atgtcggaca gctgcatggc ggtattgcta cttatggtaa ggatcctgaa
      721 gtccaaggag aattatggga cggcaagatg tatgtgtttg atgaacggat cgctgtcgat
      781 atcaaccaag tagataaaca aatcgtcggt cgcgactggt ttgacggaac accatgtgaa
      841 cgatatatca attgtagcaa tcctgagtgc aatcgtcaga ttcttgcttc cattgaaaat
      901 gaagaaaaac atttaggagc atgtagcata gagtgtgcga aacatccgcg caatcgctat
      961 gttcaagacc atggaatctc tccagaagaa gcagaactgc ggtttacagc tttaattaaa
     1021 tagaaaacaa tagttttgat tgtcgaccat ttccaatatg cgctatacta gcgttgagga
     1081 tggtgatcca catgaaatca ttgatttatg ctattgggga tgtccatggc aaacatgagc
     1141 tttttgaaaa acttattcac tattatgatc caaagatcca tcagcttgtt ttattagggg
     1201 atctcaatga tcgaggtcct cgtacaaaag aatgtttttt attaggaaag caattagtcg
     1261 aagaaacagg agccatttac ctccggggaa accatgaaga atattttttg caatttctgc
     1321 aatcaccgga ggactggtat gcgccttata tgagaaatgg tggaaaagaa acgtttgaaa
     1381 gcctgattca cccaggggca tcggaggaat actccccaac tgaaatctcg atgatgatcc
     1441 aatcgagata ttcagaactg attaaatttt tagtccagcg ccccttgtat tatgaatggg
     1501 aaaattatct ttttgtccat gccggtgtgg atttgacaaa gaaagactgg cgtaagacaa
     1561 gtctaagaga ctttatttgg ataagggaac catttcatga agggaaaaac aatacaggta
     1621 agacaattgt atttggacat acgattacac caatgctcca tggagatatg cagacaacag
     1681 acctctggat tcaggatcat aaaatcggaa tggacggtgg ggctgtattc ggagggtcat
     1741 tgcatggggt tgtttttgac aaggaaggta ttcttcaaga tattgaatat cagaacaacg
     1801 aaggaccttg gcatggaatc tgactaatgg aaagaatgaa ggagctgtga ctttgttgcg
     1861 gctcttttta tttttataga gaatattgtt tctttcagta gtttaaccca ggataaaaag
     1921 tatgatagaa taaagaagca atttttagca gaaaagaggc attttaatga cggaaaattt
     1981 gaaccagacg atcattcagc ttgtacaaaa agaactttca gactatcgtc ctaagcagtt
     2041 gactactgta cttaatttgt taaatgaagg aaacactgtt ccttttatcg cgcgttaccg
     2101 aaaagaaatg actggaagtc ttgatgaagt ccagattcgt gaaatcgaag agcggtatgc
     2161 ctatcttgaa aacttagaaa aacgtaaaac tgaagtgatt cggctaatcg atgaacaagg
     2221 gaaactgact cccgagttag aagcagaaat cacccaagcc gtgaaaatgc aacaagtgga
     2281 agatttgtat cgtccataca aacaaaaacg acggacaaaa gcaacgattg ctaaagaaaa
     2341 aggacttgaa ccactcgcga agtggctgat gcagcttaca gatggagagg tgcaatctga
     2401 agcagaaaaa tatattgaca aagaaaaaga agtctcttct gccgaagaag cgttgcacgg
     2461 tgctcatgag attattgctg aacaagtcag cgataatgca aaatttcgga cttggattcg
     2521 gtcttatacg tataacaaag gtatgtatgt cagcaatgtc aaggatgagc aagcagatga
     2581 aaaaggcgta tatgaaatgt attatgactt tgcagaaccc gtccataaaa tggtttctca
     2641 tcgaatatta gctactaatc gtggagaaaa agaagatatt ctgaaagtat tctttcaagt
     2701 agatgaagca gcaattttag cttacttgga tcgccaaata gtcaaaaatc ctgcctctcc
     2761 ttcttcttca ttcgtcagag aagcctatca ggatagttat aaacgcttta ttcagccagc
     2821 aatcgagcga gaactgcgca atgaattgac tgaaaaggca gatgagcaag caatcgctat
     2881 tttcggcgaa aacctccgta atcttttgtt gcaaccgcca ctaaaaggta aagttgtcct
     2941 gggatttgat ccggcttatc gtactggatg taaactagca gtcgtagatg cgacagggaa
     3001 agttttagca atcgaagtta tttatcctca taaacctgca gcgcaagcaa aaagagaagc
     3061 tgctggacca gcttttatcc aattaattaa taaatatcaa gtagatatgg tagctatcgg
     3121 aaatggtaca gccagtcggg aatctgaact ttttgtggct gagcagttga aatccgccga
     3181 ccataaaacc tattacgcta tcgtcaacga agcaggcgct tctgtttact ccgctagcga
     3241 aattgcacga aaagaatttc cgcatctaca agttgaagaa agaagtgctg tttctattgc
     3301 ccgtcgcctg caagatcctt tagctgaatt agtcaaaatc gatcctaaag ctgtcggagt
     3361 aggccagtac caacatgacg tttctcaaaa acgtttggct gaacaattgg attttgttgt
     3421 ggaaacagcg gttaaccaag taggtgtgga tgtgaataca gcaagcccgc agctcttgca
     3481 gcatatatca ggattgaata aaacaactgc ccaaaacatt gtcatttatc gtgaagaaaa
     3541 cggagaattc accgcacgaa ctcaattgaa aaaagtgcca cgcttaggac caaaagcata
     3601 tgaacaagca atcggattct tgcgagtccc tggtgggaaa aacattttgg acaacacagg
     3661 tatccatccc gaaagttatt ctattgcaaa agaaatatta acttcagtcc aactttcaga
     3721 aaaagagctt ggtacagaag aagcagtaga aaaattgaca cgattatccg tagagaaact
     3781 ggctgaaagc ctatcagtcg gagaagaaac gttagccgat attttagctg ggctgacaca
     3841 acctggacga gatatgcgtg atgagatgcc agcgccctta ttacgtacag atgtattgag
     3901 tatggaagac ttgaaaccag ggatggaatt gactggaacg gtccgtaatg tcattgactt
     3961 cggcgcattt gttgatatcg gtgtgaagca agacggactt gtccatatat cgaaactcag
     4021 caaaaaattc gtcaagcatc ctacagatgt cgtgtcagta ggagatatcg taacagtttg
     4081 gatagaacaa gtcgatacga aaaaaggcag aatcagtttg acgatgcttt ccccttatga
     4141 agagtaacaa tgaggagtaa caaaaattga cacaagaaga actgcagcag ttagtagaag
     4201 agatatcatt aaaaagcttc cagcttcctt tcaagcacca agcaatgttc aatccacgct
     4261 tacgtacaac tggcgggcgc tatcatttaa acgatcatca tttagatttt aatgcgaagc
     4321 tatttgaaga gtcagacgaa gagacgattg caggaatcat caaacatgaa ctttgccatt
     4381 atcatcttca tctagctgga agagggtatc agcataaaga tatggatttc aagtatcttt
     4441 tgaaaaaaac aggagggttg cgctatgcac cagccttgac gaagaaacag ataaagatgg
     4501 agtattatcg atgccaagat tgttctgcca cgatctgccg caagagaaga atcgatgtac
     4561 gcaaatatcg ttgtggaaga tgtcatggag aaatcaaatg gattgagaca aaaagagaag
     4621 actttaatga agcacaaaaa atcagatgaa aggatagaag gcctttggaa aaaagtgagc
     4681 agaggaatct aaaagactgg aatcagctat taaatttttt agccgcacct gaagagggaa
     4741 attttgagga actgacggca cctacactta ttttagctgg aaactgtctt cctatcttag
     4801 ctgataaagc agcacaaatg tatttaaatg gccaagtaga tcaattgttt ttggtcggag
     4861 gggtggggca tgcgactaga atcctttatg agaattttga aaagcaagga tttcattttg
     4921 aagatggcat gagtgaaagt gaaatctgtc gtcagtatct aaaagaagtc tatgatttgc
     4981 ccgataaggc ttttctgata gaatcgaagt ctacaaattc tggagagaac gccacttttt
     5041 cactggagat tcttcattcc ttaggagctg taccagaaaa aatcttattg atgaatgatc
     5101 cgacactgca acgcaggact cgagcaactt ttgagaaagt atggcaaaat gaacaaacta
     5161 ttttcgcgaa tgctgtgcca tttgtgccgg aaatccttca cttttctgaa gagatcatct
     5221 ttacagcaaa agaactgaat catcaatggc ctaaggaata tttccatgcg ctagtattag
     5281 gagaaatgga acgtctccat gacgatgaga acggctatgg tccgaaagga aaagatttta
     5341 ttcctcacat tgatatatca gaggaagttt ggtcttctta caccagaatt aaagagtcaa
     5401 taaaaacgga tttttctcgt acttgaccat tttagcaagc gaatcctcct ttccaaataa
     5461 atttggttga aattttcaat cgtttataag ataatgagga gcgtgttgaa tgtaatagct
     5521 aaaagaatgg acgctagata agagaagagg attcaaatgg aaaatcaaaa taaattgtat
     5581 cattttgttg gtatcaaagg ttcaggaatg agctccttgg ctcttgtttt acatgaacaa
     5641 ggtctaaatg tccaaggatc agacattgaa aaatatttct ttacacaaag agatttagaa
     5701 aaagcaaata tcacgattct gccatttaac gcagataatg taaaaccagg gatgacgatc
     5761 attgcaggaa atgcatttcc tgattcacat gaagaaatcc agcgagcaaa agaattaggt
     5821 ttggaagtga ttcgctatca cgactttatt ggtcatttta tccaaaatta tacgagtatc
     5881 gctgtaacag gctctcacgg aaaaacaagt acgactggac tgctctctca tgtattgagt
     5941 ggtgtccgtc caacaagtta tctaattgga gatggaacag gacatggtga tccgcaagcg
     6001 gaattctttt catttgaggc ctgtgaatat cgccgtcatt tccttgccta ttcaccagac
     6061 tatgcaatca tgaccaatat cgattttgat catccggatt attatacaag tatcgacgat
     6121 gtatttacag ctttccaaac aatggctgga caagtgaaaa aagctatttt tgcgtatggt
     6181 gacgatgctt atttacgcaa attaaaagca aatgtgccga tttattatta tggtgtcaca
     6241 gaaaacgatg atatccaagc acgaaacatc gaacgcacaa cttctggttc tgcttttgat
     6301 gtctatcatg gggatgaatt tgttgggcac ttcactgtac cagcattcgg taaacacaat
     6361 atcttaaatg ctttaggtgt tattgcagta gcttatttcg aaaaactgga tctgaaagaa
     6421 gttgcagaag aaatgctgac tttcccagga gtaaaacgtc gattcagtga aaagatcgta
     6481 gcagacatga ccgtagtcga tgattatgcg catcaccctg cagagatcaa agcaacgatt
     6541 gatggcgcac gccaaaaata tccagataaa gaaataattg cagttttcca accccatacg
     6601 tttacgcgta cgattgcatt gatggatgaa tttgcagaag cacttgattt agctgataaa
     6661 gtttacttat gcgatatttt tggttcggct cgggaagaac aaggaaacgt taagattgaa
     6721 gaccttggag cgaaaatcaa aaaaggtgga gaagtcatca aagaaaataa tgtatctcca
     6781 ttgttagatt atcatgatgc agtagtaatc ttcatgggtg ctggagatgt tcaaaaattt
     6841 gaacaagcgt atgaaaaact tttaagcagc acgacgaaaa atgtactgta atacaattac
     6901 tgcagtgtcc ttataaaaat agaaagaggg ggagtggcaa aaatcttgcc aagccccctt
     6961 ttttgcaact tatgataaac ggtgttcaaa agcttgcatc tgcggtaata agcccaatca
     7021 aatcaaaaac atgagaagct gttttttctt ggctgttctt attatttgat gcaggacagc
     7081 tttttcacga cctctgtaaa ctataggaat ctgttaaatc aagggagcag aacttgcctt
     7141 ttacgctact tttggtacaa taatgtatga ttaaaaataa actggagggt ttctatcttg
     7201 aacataggta agccgagaaa actaggaaaa acaataaatg aaatcgagga aggcgactcg
     7261 ttatccttaa cagaatctat tgaagataac cagcttttgc tgtatttggg attgacgaat
     7321 gatgcaaatc ctttgtatat ccaacatgac tttgcacaaa aaacagaata tcaacagcca
     7381 atcgttccat cagtgatgct gatggggatc atcacaagtg caatttcaaa gcatcttcct
     7441 ggaccaggat cacatgttgt caatttttct gtcaatttta ttgaaccggt tttccattat
     7501 gaaacactga cttttcagtt tgaggtcata aaagtagata agatgaagga tgtggtgacg
     7561 atttctgtag aagcggagaa tacgaacgaa gatcgtgtgt tggatgctgt cgttatggtc
     7621 caaccgccac aagttcctga attggaaata gcaatcgaaa gtgaaggtga attgaatgaa
     7681 taatccaagt acatcaatgt caggtatcaa tcgtttttat gcaaaagttt atggattttt
     7741 aggtatggga cttgccctta gtgcagtcac ttcttacttg gctctttggg tatttccttt
     7801 gcaattagca acttttatca ataattttcc actaggcttt gtgggtctgt ggatcgttga
     7861 actgatttta gtaatggttc taggcgtaaa agcacaaaag aatccgagct tagcaatcgg
     7921 tggatttatt gcctactctc ttttgaatgg cataacatta gcggttactc ttgcatacta
     7981 cgatattgga acagtaacgc aagctttctt gacagctact ttgatgttca tggggatgtc
     8041 tgttgtcgga gtaacaacaa aaagagatct tagcggggtt ggaagagcag gacaggcagc
     8101 tttaatcgga gtgatcattg cactagtctt gaacatcttc attttgagaa gtagcgtagt
     8161 tgagctgttc atctccctac tgttagtcgt catctttgca gggattacag cttatgataa
     8221 ccaaagcatc cgtaatatct atgtccagac aaacggaaca gcacatagtg gaattgctgt
     8281 attcatggca ttgaagctgt atttggactt tatcaacttg ttcttggcgt ttctgagaat
     8341 ctttggacgg aacaactaaa aaaaaaaaaa attcatcagt tggggctata ccgaattatt
     8401 tggtatggtc ccgctttttc ttttcgcatt ccgcaggatt ctttgttaga atgaataaga
     8461 actttacagt aaggagttaa gaagatgaca aaaaataaat tattactcgt tgatgggaac
     8521 agtgtggctt ttcgcgcatt ctttgcgctt cacaattctc ttgaacgttt caaaaataga
     8581 aacggtcttc atacaaatgc aatctatgca ttcaatacga tgtttgaaaa tgttatgcaa
     8641 aaagagcaac cgactcatgt tctggttgcg ttcgatgcag gaaaaacgac ttttcggaca
     8701 gaaatgtatg cggagtacaa aggtggaaga tccaaaacac ctggcgaatt caaagaacaa
     8761 atgccatata tacgtgattt gttgacagga ctaggggtac agtattacga attgcccaat
     8821 tatgaagcag atgatattat cggtactttg gcagaaaaag tagacaaaga tcaatttgat
     8881 gtcgtcgtgc tttcaggtga tcgggatttg actcaattag ctagtaaaga agtaaaagtg
     8941 gatatcactg taaaaggtgt cagcgatatc gaaagctata caccagaaca tgtagcggaa
     9001 aaatatgatg ggttgacgcc aaaacagatt attgacatga agggactagc cggagatgcc
     9061 tctgacaata tccccggtgt gactaaaatc ggtgaaaaaa cagctatcaa gctattgaaa
     9121 caatatggct cagtcgaagg aatttatgaa catatcgatg aaatgaagca aagcaaaatg
     9181 aaagaaaatc tgataaatga caaagagcag gctttcttat cgaaaaaatt ggcgaccatc
     9241 aatacttcag cgcctgtgga tgtctctatc gactcattga agtatgaagg gaaaaatctc
     9301 gataaactcg ttccgtttta taaagaaatg gatttcaatc agttcctttc taaattgaat
     9361 atcgtagaag aagaagtaaa aatggatgat attttgtttg aagtcgttca tgaattcaaa
     9421 gaggaaatgt ttactacgga tatggcgttg tacgtggaaa tgatgggcga caattatcat
     9481 acagaggaaa tcgtcggagt agcgtggggg acagaaaaga aaatttatgt cacgaacgat
     9541 ctatctgtat tcgaaaaccg agcgttccat tcttggatca ctgatccgac tcggctgaaa
     9601 aaagtatatg atgcgaaacg aacttatgtt gctttaaacc gctatactgg aaaaacgaaa
     9661 ggaatcgatt ttgatgtctt gctaggtgca taccttctcg atacgaatga aaaaagcaca
     9721 gatatcgaag gaatagctgc tcattacgga tacaacgata tccaatcaga tgaagctgta
     9781 tacggcaaag gggcgaaaaa aggtttacca gaagaagagg aactcttttt tgctcattta
     9841 gcgcgaaaag tagctgcaat caatgcgttg acaccaaaat tgagccaaga actggcagaa
     9901 aaaaaccaag aagatctttt ccggaaaatg gaactgccat tgtctatcat tttggcagag
     9961 atggaaataa gcgggatcaa agtagatgcg actcgcttac aagaaatgaa gggcgagttc
    10021 tctgctcgct taagagagat cgaacaaaaa atctacgaag aagccggcga agaattcaat
    10081 ttgaattcac caaagcaatt aggagtgatc ttatttgaaa aaatgggatt acccgttatc
    10141 aaaaaaacaa aaactggata ttccacagcc gtagatgtac tggaacagct tcgtgaacaa
    10201 gcaccaatcg tagaggatat cctcacctat cgtcaaattg caaaaattca atctacctat
    10261 gtcgagggac tgctgaaagt gatccaatca gatggaaaag tgcatacccg ttatgtccag
    10321 acattgaccc aaacaggacg attaagctct gttgatccta atttgcagaa catcccgatt
    10381 cgattagatg aagggagaaa gatacgtcag gcatttgttc ctagagaaaa agactggctg
    10441 atttattctt ctgactattc acaaatcgag ttacgcgtac ttgcgcatat ctcaaatgat
    10501 gaacatttga aagctgcctt cctagaaggt caagatatcc atgccagcac agcgatgcgt
    10561 gtatttggaa tcgaaaaggc agaggatgtc acaccaaata tgcgtaggca ggcaaaagca
    10621 gtgaattttg gtatcgtgta tggcatctct gattacggac tttcacaaaa tctaggaatc
    10681 agccgaaaag cagcacagca atacatcgat acgtactttg aaaaataccc aggtgtcaaa
    10741 gaatatatgg aaagaatcgt acgcgaagca aaagaccaag gctacgtcga aacgctgtat
    10801 caccgccgtc gatatctgcc agacatcaat tcaagaaatt acaatatccg ttcttttgcg
    10861 gaacgaacag ctatcaatac accaatccaa ggaagtgctg ctgatatttt gaaaatcgcg
    10921 atgatcgaat tggataaacg attaaaagaa acaggtcttc aagctacgat gctgttacaa
    10981 gttcatgatg aacttgtctt cgaggtaccg gaaaaagaac ttgaatcatt agataaattg
    11041 gtgaaggaag ttatggaaca agctgtttct cttcatgtac cactgatcac tgacagcagt
    11101 tggggaaaaa cgtggtacga agcaaaataa acattgttta agacagagca atataatgaa
    11161 ggtgaaaaga aaaaatgcca gaattaccag aagtagaaac cgttcgtaaa ggattggaac
    11221 ggttagtagt aggaaaaacg attcaaaaag ttcaggtctt gtggcccaag atcatcgagc
    11281 aaccagagac acccatcttt gaggcttctc ttgttggcga aaccatccaa tccattggac
    11341 gtagagggaa gtttttaatt tttcatctcg atcattgtga actgatctca cacttgcgaa
    11401 tggaaggaaa atatcaattt acgaaagaaa atacaccaat cgataaacat acgcatgtac
    11461 ttttcttttt tgaggatgga agccaacttc gctacaacga tgtacgaaaa tttggtcgta
    11521 tgacaatcgt cgaaaaagga gcaagtgcaa cttaccgagg aatcatgaaa ttgggacctg
    11581 agcctttgcc agatagcttc ttattagcag atttcgcaaa tggactgaaa aagtcccaca
    11641 aagcaatcaa gccattgctg ctagaccaac gtctagtgac tggtctaggt aatatttatg
    11701 tagatgaagc attatgggaa gcgaagatcc atccagaaca accggcgaac acactgcgcc
    11761 ccaaagaggt agaacaatta cgtctatcga tcattgatgt tttggatcgt gcaatagaag
    11821 ccggaggtac cacgattcgc agttatttga atgcattagg agaatcaggc ggatttcaag
    11881 tagcactgca tgtctatcag caaactggca aaccatgtat acgttgtgga acgccgatcg
    11941 tgaaaacaaa agtggcacaa agggggacgc actattgtcc gaattgtcaa aggctaaaaa
    12001 tcgcaaagta ggatttgttt taggtttgac gggtggaata gctaccggta aaagtacggc
    12061 agccaaagtc tttcagtctc atggatttcc tttgatcgat ggcgatgtga ttgccagaga
    12121 aactgttgag cctggaacgc ttgctctgag gaaaattgta tcagtgtttg gccaagaggt
    12181 attgcagcct gatgggcaac tagatcgagg gaaacttggg atgattgttt ttccatcaaa
    12241 ggaaaaacgg caaaaacttg atcagctatt agatccgttt atccgaaaag cgataaaaga
    12301 tcaaatcgcc tctctaagtt ccaatcatcc tttagtaatt gtagatatcc cattgctcta
    12361 cgaaggtcat tatgaccact atatggatgc agtagctgtc gtttatacta cgccagaaac
    12421 tcagcttcag cgtttgatga aacgtaatca actgacgcta gaacaagcgc agcagagaat
    12481 tagtagtcaa cttccaatcg aagaaaaaaa gcagcgtgcg gatattttgt tcgacaacaa
    12541 tggaacaaaa gaaatacttg tgggacaaat cgaaaattgg ctttttgcta atcgctttct
    12601 ttgagtatct gtgtcaattt tgctatcctg agtatataga aagtgaggga tagcgatggg
    12661 atcattaaaa aatcaaaagg ataaagtcgt aggtaacgca aaagaaaaaa tcggcgagat
    12721 gctcaataaa gaaaatctaa tcgatgaagg tcgtacgcaa tcagaaacag cagaagaaaa
    12781 agaaaagcag taccaagcaa gaatcgatga aatagaagac cagcaggcag aagaagaagc
    12841 gaagcggcaa gatttgactc aagaacgaaa aaatccgaca cctgatcgta gtaaaacacc
    12901 gctcccagaa agtgaccaca ttcccgatga gcaaaaaatc gaggaacagc gaatgaaaca
    12961 ctatacagga atcgaagaga agttataaaa tagtaaaaaa tcgagatgtg acttgaagca
    13021 gatgagaagg tgttcatctg cttcaagttt tttgtgctag atgactaaat cagcttttgt
    13081 gtaagttatt tctcagtaat caaggtgatc agtattggaa aattgaaatt tagtaaggac
    13141 aggggacaca attacgcatg aaagtagaaa aaattgttct tatatccgtg ttttttgggt
    13201 aggacagtcg cgtttgtgat ttgtttcgtg ttataataca actagttgta aagaaagaca
    13261 catcctttat ataaaatgcg atgcaagagg tgagacaaaa tgagatgtcc cagatgtcaa
    13321 cataataatt cacgagtaat cgacagccgt caagcagatg atggacgtgc gatccgtcgc
    13381 cgtcgcgaat gtgagaattg ttcgttccga ttcactacat ttgaacgaat tgaagctgct
    13441 ccgttattag taatcaagaa aaacggggaa cgcgaagagt tcaatcgcga taaaatttta
    13501 agaggactca tccgttcagc agaaaaaaga cctgtagcta tggaacaaat ggaacaaatc
    13561 gtcaaccatg tcgaaaaccg cgtacgcagt ctcggagaaa acgaagtatc gacgaatcta
    13621 attggtgaat atgtgatgga agatttagtt gatttagacg aaatcgctta tattcgtttt
    13681 gcaagtgttt atcgccagtt caaagatatg tctgttttct taaaagagtt gcaagatatc
    13741 gtggataaag caaaagaaaa taataaagag aataataaag aaaacaatta agaaacaaaa
    13801 aggaggcatt tccttggaaa atgcttggaa agagcttcaa ccaaaaaata tttaccaagg
    13861 aagaaaagcg gctccgctga gagaagacag caagaacgcc ttgctgtatc tttatcagcc
    13921 actaatcggc ggagaagcct tgagtatcta tttcaccctg ttatcagaaa tctctcaaga
    13981 atctggcaaa ggtcctgaag ggatgcatgc agatctgctt agtagtctag gctgcggtat
    14041 cccgcaattt tatgatgcaa gaaaaaagct agaaggaatc ggcttactgg atgtttattt
    14101 taaagaagat agtgatctgg gacctcgttt tatttatgag ctgttagaac cactcattcc
    14161 taaagcattt ttcaaagact ctgttctggc ttttcttctt ctggaaaaag ttggtgaacg
    14221 acgattccag aagcttaccc aattctttga acctaaaagg catacagcag agggatatca
    14281 aaaaatcaca aaaaaatttt tagatgttta tcgcttcaat gaagcagtgt acgctgctaa
    14341 tcaagaaaac atcgaatcgc tgaataaatc ttttgaagaa tctgaatcag tagcattact
    14401 tgaagcagat tcgacattcg attggcagtt tttgaatgat tggctggcaa aaaaacatat
    14461 tgcaagtctt gatgatccaa tcatcaaaca agtcgacatg tatcatcagc tttacggttt
    14521 cgatgaattg acacttggcg aactgatcag tcaatcttat gactttagtg aacaaaaagt
    14581 atctgaaaaa gaactgcaaa aagccatttt ggcaatgcag agacgtccgg tgaagcaaac
    14641 aaaagaaaca gttgcggttt ctgaaaacag taatgaaccc tcgtcaaacg aacatttgac
    14701 aaacgcagcg cttcagctga taaaagaagc acagagcatt ccaccgatga aatatctgga
    14761 agcaatcaag tcagaaaaag gcggatatgt ttctaaacaa gaaaactggt tactgcaaga
    14821 tcttgtttct caatcaggtc tttccagtag cgtgatcaat gttttgctga attatgtact
    14881 agtgatcaag aataatgcta gcttaaatgc cagttatgta aatacagtgg caaatgaatg
    14941 ggcacaaaag aaaatcgcca cagcagaaga agcgatccag cacattcggc aaataagtaa
    15001 tcaagcaaaa caatcaaaac aaaagaagca aacaaattat caaactggca agcgaaatgt
    15061 tcgacgggaa aagcttcctg attgggtcaa ccagccaaaa gatgaaaaac agatcagcag
    15121 cgagaaaaaa gcagagattg accgccgttt taaagaatac ctggcaaaaa aggagggtga
    15181 tatctagtgg aagacgtagg caaagaattg aagaggatca tcagcaaacg caattatcaa
    15241 gaaaagtatg ctgaaatgat cgctgaagtc ttaaatgatg cggatgttca agcttttcta
    15301 agcgcacacc aagaagaatt gacacaagcg gatattgaaa aaagttacgc taaattgtat
    15361 gaattcgtgc aagaaaagcg taaatatcaa aaaaacgatc caacgatgat cgctcccggc
    15421 tatgagccga agctgacgtt gaattttcat tatgtggatg tcacttatgt accaacggaa
    15481 gcattgttag cacggcaaag acaagaagaa atcagaagcc gagtaaaagc aatggatatg
    15541 ccaaaagata ttcaatcggc ttcttttgaa aactttgaac gtacagatgg acgaggatat
    15601 gcgagcttag aagccctaga ttttgtagag aaatatactg ctgatccaaa agggtatcat
    15661 aaaggactgt atctagtagg aagttttggt attggaaaaa catatttatt aggagcaaca
    15721 gcgagagaat tagcgattgc tggattcacc acgacgctag tccattttcc gacatttgcc
    15781 gttgagatga aacaggcaat cggaaaagat caagtggcag aaaagttaga tgcagtgaaa
    15841 cgttcaccta ttttgatgat cgatgacatt ggtgcagatg cgatgagtag ttggatacga
    15901 gacgaagtat tcggtgttat ccttcaatat cggatgcagg aacagttacc aaccttcttc
    15961 tcatcgaatt ttacaatgga tgaattggaa cagcatttgt ccgtcactca acgtggagat
    16021 gaagagccgc taaaagcgaa acggataatg gaacgcatac gttatttgac aaaagaaatc
    16081 gaaatgactg gtcgcaaccg acgcaatggt tgaacagttg acaagtccaa ctgacatttg
    16141 acctagtagt aaaagcaggt cagcgaatca ttatatggac agaaatgacc ggagacaatc
    16201 accgcaacaa gtaatgcaga aacttaaacg gaggattttt tatgagggct attttttcta
    16261 gtatatctgt agttggtcta ttttttcttt tagcaggctg cggtagcgca gatagcaaca
    16321 caggttctgt tgattcgatt gatgcgggtc aaacagtaaa aacaagcagt acgaaaacgg
    16381 agagacaaag catatttgtc ggaacgattg aaagtatcac tcaagtagaa gcagaagaag
    16441 aaacgcttca gttgaaactc gtcgatgtca tggccaaaga agatccagcg aatatgggaa
    16501 cgtcattcag caatgacgga gtgatcttaa atgctactgc tgaaaaaata agcgtggaca
    16561 taaatgaatt gaaaatcggt gacaaagtcc aatttacatt agttgagatg ccgatcatga
    16621 cgatgtctat cccaccgcaa gttcctggag attcgattat tgatgtttca ttgaacaaat
    16681 aaccaataac attttttcaa aaataagaaa atgttgtttt atagaacgaa aaggtatatt
    16741 ttttaaggag atatgggaat ttttttattc ctagtctcct tattttattt ggaaaatgct
    16801 ataattatct agaaggagtg aagctatgaa ccatttagat ttatctcagc gcttagccgc
    16861 agtgggtgac tttgtccctg aatccgctcg tttagcagac attggatcag atcacgctta
    16921 cttacccgtt gcattgatat taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt
    16981 tcaaggtcct tatgaatcag cacgtaagca agtcaaaaaa gatggattag aagaccgtat
    17041 caccgtacgc cttgcaaatg gtctagatgc tatccagcca caagatggta ttacagctgt
    17101 cacgattgcg ggtatgggag gttcactgat ccgagatatc ctagaagctg gcaagttgaa
    17161 tcaacgctta agcggacaag aacgtttgat ccttcagcca aacatcggag agcgtacatt
    17221 acgatcttgg ctgcaagaaa atcagtataa aatccaatca gaaaaaatac ttgaagaaaa
    17281 taaaaaaata tatgaaatca ttgtagccga aaaagaacaa aaaccagtta cttacaatga
    17341 aatggagctg atgttcgggc catttctgct tgaagagaaa agtgagatct tcaaatcaaa
    17401 atggacaaga gagcttcaac aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa
    17461 tcaagaggca cgtcaaaaac aaatcgagca ggaaatccaa atgattgagg aggttttaaa
    17521 gccatgacaa tcagcggacg cactttcatc caaaagtttg aaagctattg tcctcagtgg
    17581 ctagctgaag aaggagaccc agtcggactg catatcggaa cattggataa gccaatccaa
    17641 cgagtaatga tgacattaga cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa
    17701 atcgatttgc tgattgctaa acatcctcca atctttcgtc ctgtaaaacg gctggttact
    17761 gatcagccac aagaaaaaat gtatgcagat ttgttgaaac atgatattgc cgtctatgca
    17821 gcacacacga acatggatat tatttgggat ggactgaatg actggttttg tgaacttctc
    17881 ggtattgaag tggagagtta tttagtaaag actcatgaga ttcattataa aaaactggcc
    17941 gtttatgtac ctgtggatca tgcacagaaa atgcgtgaag tattagctgc agctggtgct
    18001 ggtacacaag gtgattatac cggtacaagt tttacctcga ttggacacgg tcgatttact
    18061 ccggaagctg gtgcacaacc agcgattggg aaagtaggga agacagaaca agttcaagag
    18121 gcaaaagttg aggttatttt gccggaaact atcgaaaaac aagtgattca agccatgcgg
    18181 tcagctcatc cttatgagga acctgcttat gatcttttcg ctatagatga accagtcgaa
    18241 atgtttgggt taggacgagt aggtgaattg ccgcaagaaa tttctataga ggcatttgta
    18301 gaacaagtaa aagaagcgtt ccaactagat ggactgagaa tagtccagcc aaaaaatgca
    18361 aaatcctctg tgaagcgcat tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa
    18421 gcaatcgccc aaagagcaga tgtctatatc acgggagata tctattatca tacggctcat
    18481 gatatgcaaa gcgctgggtt gattgcaatt gatcccggtc attatattga atcgttatgc
    18541 aagcaacgat ttatcgagaa atttgaatct tggaaacaag aagaaaattg ggatattaac
    18601 tttttcgttt ctgaaacaaa tacaaaccca tttcaattta attaaaagga gagaatacta
    18661 tgtacgaaaa tcttttgcca cgttttttac gatatgtcaa aacagaaaca cgctccgatg
    18721 caacaagcaa aacgactcca tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa
    18781 aagaattaga agagttaggc ttagctgatg ttttctataa tgaagataac ggattcgtta
    18841 tgggaacatt accaagcaat gttgatcatg aagtgcgttc cattggattt atttcccaca
    18901 tggatacagc agattttaat gcagtaggag tcaatccaca aatcattgaa aactatgatg
    18961 gcgagtctac gatcgtacta gatgcggaag aacgctatac attgaataca aaagattttc
    19021 caaatttgaa aaattatgaa ggccacacat tgatcacaac agatggcagt acattactgg
    19081 gagcagatga caaatcaggg attgcggaaa tcatgactgc aatggagatc ttgatcaata
    19141 atccttctat ccctcacggt accatcaaag tggcttttgg tcctgatgaa gaaatcggtg
    19201 tcggagcgga taaattcgat gttgagcaat tcgatgtaga ttttgcttat acagtcgatg
    19261 gtggtccttt aggtgagttg cagtatgaaa cgtttagtgc tgctcaagca aatattacga
    19321 tccaagggaa aaatgtacat ccagggacgg caaaagatac aatgatcaat gctctacagt
    19381 tagcaatcga ttttcataat cagcttccag ccgatgaagt tccagaaaaa acggaaggat
    19441 acgaaggttt cttccatttg atggcattgg atgggaatcc tgaagaagct tctatgagtt
    19501 atatcattcg cgatcatgat cgtgaaaaat tcgaagcacg caaagcacaa atcacacata
    19561 tccaagaaga gttgaatcag cgatttgatc aagaacgaat caaagtagat atgtatgatc
    19621 aatattacaa catgaaagaa atcattgaaa aagatatgag catcgtggaa ttagcgaaac
    19681 aagcaatgat cgagcttggt atctctccag tgatcgaacc agttcgtgga ggaacagatg
    19741 gttcaaaaat ctcttacatg ggtattccga cgccaaatct atttgccgga ggagaaaata
    19801 tgcacggacg attcgaattt gtttctttac aagtaatgga aaaagcaaca gacgtgattg
    19861 taaaaattgc agaattgaac acaaagagtt aaaatcgaaa aagaacagct tatgacaagg
    19921 cgttcgtccc aatgatggaa tgtcatctgt ctaaggctaa agggcaggac aatggaattg
    19981 gttgtcccgc cctctttttt gaagagtaaa taggaggtaa agaatggaaa agctgctagt
    20041 tttttataac aaaagttcag ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga
    20101 ttatgtaaaa gaaaagcagc cagaattata tgtagaacta gctgaaactg gcccatcgat
    20161 tgatgatgcg gaattagtca agaaagcaga agccatagaa gctgatactt tagtaattat
    20221 aggtggagac ggcacgattc atcatatcgt ccaagcgttc caaaaaacat tatctcaata
    20281 ccatgtcgga ttacttccag gtggaactgt caacaacctt gcacgtgttc ttgagattcc
    20341 cctcaaaaaa gaagaagctg ctgacgtgat cctggaaggc aatgtacgga aaatagacta
    20401 tggtcaagtc aatgatgaag tcattatctc tacactgaca atcggaatct tagcagatac
    20461 agcagtttgg atcagtcaag aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa
    20521 gttttttaag ttattgctga agaagaaaaa atatcatttg gatttagaaa cagacgagga
    20581 gcattggaaa ggaaaatccc aactgttgac agtcacgatg tctaactccg tgggcggttt
    20641 tacaaatttt gacgactcag cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc
    20701 ttccttagat attttccgat ttatttttta tttgcctaga atcatgagag gcaaattcta
    20761 tacgatcccc ggcattactt atttcacagc caaacgcatg aaaatccaag cgcaggaaaa
    20821 atctgtgggc actcgaacgg atggagacac gacagatgat cttccagttg aattatctgt
    20881 tgtatcaaaa ggattaacag tttattcacc aaagaaagaa gaaaaagctt agaaatagta
    20941 gttaacagaa agttggaaga agagtaatgg atgtattgtt acacaatcct ttgtggtttt
    21001 accttttcat tgtgaatttt tacttatttt gtctgatggg gtatgataaa catcaggcta
    21061 gaactagcgg ctggcgtgtt cctgagaata atttgatatt tgtcagcctg ttaggcggtg
    21121 gtttgggagg attgatctct cgaaaagttt tccatcataa aactcggaaa aagaagttta
    21181 ctataggatt cgctgcaggt gtacttgttg atttgattct catttatttt ttccattaag
    21241 gagcaggaga tgtcgtctaa aggaacgaaa actaaaatca ttctgaatct gcttttgctg
    21301 attgtgattt ttgccattat ttattattta atcaatcaat catttgcaga tatctttgcc
    21361 gaattgctgt ctaccagtgt tgttgttttg gcagcggctg ttatattagg gacagtttac
    21421 caaatcgctg aagggcgctc aatcaaagaa attgctgaac ctttcgcgcc gaattttaca
    21481 acgaaagatg ggttcttgac ctctagctat attgcgtttt accgaatcgt ttcgtttggt
    21541 acaggaacgc tgatctcgga gatttatttt tataacaaaa aaggaatgaa gctatcccag
    21601 ggagcagggg tgacagcact ccatatgatt atgtacaaac tggcagtgat cacttatgca
    21661 ttagctggct tggtcatcca attctctctt ttttattcta atggtccatc aatgatctgg
    21721 tttatcatcg ctggtatcct tttgacgatc gtgattgttg cctttttatt ggcgatgtcc
    21781 gtcagcttga atgttcaaat attttttgtc agcttatcga ataagttatt taaaagtgag
    21841 cgtttgcgga aatgggtgga tacatgcaat cagcagatct actctttaag ggaaacggtc
    21901 caaaccatta tccaagacag gactgcgcta ttgcggattt atctatggaa tatgttaaaa
    21961 cttgtatttt ggtatgtact tccttatttg attctgattg aaaaccatcc taaaatagat
    22021 tttctactga ccatttcgtt tatcagcttt gcagtagtgc tttcaggggt tattcctacg
    22081 cctgctggta ttggttcctt tgaatttgtc tatttgttat tattcagacc agttgttgga
    22141 acagttgacg cagtttcttc gcttttactg tatcgtttca gttcctttat cttgccattt
    22201 ctctatggat ttgtgtatgt gattgctgaa cggcgaagag tgatcaaaga agaaataata
    22261 gaagtaaaaa aagagaataa agaaaatcaa gggtaggtct tttataagat ctaccctttt
    22321 tatcaaatca aagaaaaacg ttatggataa gataatttta cgtttgtttg gctctttttt
    22381 tatatacata caat
//
LOCUS       DAXLPI010000039        22296 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXLPI010000039 DAXLPI010000000
VERSION     DAXLPI010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.039"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            31..849
                     /gene="purR"
                     /locus_tag="ACF3T5_001274"
     CDS             31..849
                     /gene="purR"
                     /locus_tag="ACF3T5_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFD3631897.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1352..2725
                     /gene="glmU"
                     /locus_tag="ACF3T5_001275"
     CDS             1352..2725
                     /gene="glmU"
                     /locus_tag="ACF3T5_001275"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFD3631898.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2819..3790
                     /locus_tag="ACF3T5_001276"
     CDS             2819..3790
                     /locus_tag="ACF3T5_001276"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFD3631899.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3796..3898
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3964..4533
                     /gene="thiT"
                     /locus_tag="ACF3T5_001277"
     CDS             3964..4533
                     /gene="thiT"
                     /locus_tag="ACF3T5_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFD3631900.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4614..5387)
                     /locus_tag="ACF3T5_001278"
     CDS             complement(4614..5387)
                     /locus_tag="ACF3T5_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631901.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5654..7354
                     /locus_tag="ACF3T5_001279"
     CDS             5654..7354
                     /locus_tag="ACF3T5_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFD3631902.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7324..7542
                     /locus_tag="ACF3T5_001280"
     CDS             7324..7542
                     /locus_tag="ACF3T5_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFD3631903.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7562..8452
                     /locus_tag="ACF3T5_001281"
     CDS             7562..8452
                     /locus_tag="ACF3T5_001281"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFD3631904.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8470..8754
                     /locus_tag="ACF3T5_001282"
     CDS             8470..8754
                     /locus_tag="ACF3T5_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631905.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            9241..9855
                     /gene="gmk"
                     /locus_tag="ACF3T5_001283"
     CDS             9241..9855
                     /gene="gmk"
                     /locus_tag="ACF3T5_001283"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFD3631906.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            9860..10165
                     /gene="rpoZ"
                     /locus_tag="ACF3T5_001284"
     CDS             9860..10165
                     /gene="rpoZ"
                     /locus_tag="ACF3T5_001284"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFD3631907.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            10418..12826
                     /gene="priA"
                     /locus_tag="ACF3T5_001285"
     CDS             10418..12826
                     /gene="priA"
                     /locus_tag="ACF3T5_001285"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFD3631908.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            12841..13332
                     /gene="def"
                     /locus_tag="ACF3T5_001286"
     CDS             12841..13332
                     /gene="def"
                     /locus_tag="ACF3T5_001286"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFD3631909.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            13325..14263
                     /gene="fmt"
                     /locus_tag="ACF3T5_001287"
     CDS             13325..14263
                     /gene="fmt"
                     /locus_tag="ACF3T5_001287"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFD3631910.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            14263..15621
                     /gene="rsmB"
                     /locus_tag="ACF3T5_001288"
     CDS             14263..15621
                     /gene="rsmB"
                     /locus_tag="ACF3T5_001288"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFD3631911.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            15634..16374
                     /locus_tag="ACF3T5_001289"
     CDS             15634..16374
                     /locus_tag="ACF3T5_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFD3631912.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            16371..18440
                     /gene="pknB"
                     /locus_tag="ACF3T5_001290"
     CDS             16371..18440
                     /gene="pknB"
                     /locus_tag="ACF3T5_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFD3631913.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            18605..19504
                     /gene="rsgA"
                     /locus_tag="ACF3T5_001291"
     CDS             18605..19504
                     /gene="rsgA"
                     /locus_tag="ACF3T5_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFD3631914.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            19517..20167
                     /gene="rpe"
                     /locus_tag="ACF3T5_001292"
     CDS             19517..20167
                     /gene="rpe"
                     /locus_tag="ACF3T5_001292"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFD3631915.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            20168..20806
                     /locus_tag="ACF3T5_001293"
     CDS             20168..20806
                     /locus_tag="ACF3T5_001293"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFD3631916.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            20961..21815
                     /gene="mreC"
                     /locus_tag="ACF3T5_001294"
     CDS             20961..21815
                     /gene="mreC"
                     /locus_tag="ACF3T5_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFD3631917.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            21819..>22296
                     /gene="mreD"
                     /locus_tag="ACF3T5_001295"
     CDS             21819..>22296
                     /gene="mreD"
                     /locus_tag="ACF3T5_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFD3631918.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFI"
BASE COUNT         7427 a         4001 c         4842 g         6026 t
ORIGIN      
        1 gataaaattt cttaaaagga gttggaatag gtgaaagtac gtcgcagtga acgtctagtt
       61 gacatgacac agtatttatt gaatcacccg catgaattga tttccttaac aagctttgcc
      121 gaacgttatg aatctgctaa atcttctatc agtgaagatt tgggaatcat caagaaaaca
      181 tttaaagaac gagggtatgg aaccttggaa acaatcccgg gtgctgcagg tggtgtattg
      241 ttcatccctg aaatttcata tgaagaggcg aaaaaatata ttgaatctct agcagaacgt
      301 ctatcagaac aagaccgtct gcttccagga ggatatgttt atctctctga tttgttagga
      361 gaacctgaac tgctgcgtca agttggaaga atcattgctt ctaaatacct tggtgaaaaa
      421 atcgatgctg taatgactgt agccacaaaa ggcgtaccta ttgcccaagc tgtgtcctat
      481 tatctgaatg cgccattcgt tattgtacgc cgggactcaa aaataacaga aggatcaaca
      541 gtaagtgtca actatgtgtc tggatcttct gaaagaattg aaaagatgga actgtctaag
      601 agaagtttga aacgtggttc acgtgtcctt gtagtggatg atttcatgaa aggcggcgga
      661 acagtcaacg gaatgaagag cttgattgaa gaattcgaat cagaacttgt tggcatcact
      721 gtatttgccg aatcaaaatt caacggtcat cgcgccatcg atgattatac gtcactgctt
      781 tatgtcaaag atgtagatac tcaaacgaaa aatattacgg ttgtgccagg aaattatttt
      841 tcagaataag aggttgtgaa aaagccgctt agtttcgagt agtaagaaaa attttgtgaa
      901 aatggctttc cacatttttt cataaatttg gcttaatacc gagaaactgg cttttgaaca
      961 ccgtttatct agaggttgtg aaatcagcgt cctgtcctga gtattagctc aataaacgga
     1021 aaaattgctc ctcatatttt gccgtttatt gagctaatac cgaaggtcag ctgattgaaa
     1081 aacgtttaat gaagaggttg taagcctacc gtttaactct aagtcacaag aaacgatttc
     1141 aaaaaacagc ttttcatgtt ttatgagtag tgtgacttgt gaccgaggag ttggcaggag
     1201 agcaccggtt accaaataga taggcccgtt atggtctatc tattttttta tccagaaaac
     1261 gctgggataa aagaaaatca cttgttttgt tgaaaaaatg ggtatactgg gataaactga
     1321 aatcagcaaa ctatatttag gagtgacgca gttggacgca cgttatgcaa ttattttggc
     1381 ggcagggaaa ggtacccgca tgaagtcaaa attatataaa gttttacatc ccgtttctgg
     1441 taaacctatg gtagaacata tcattaatcg ggtcagtgaa acgaaaccag atgaagtgat
     1501 tacgatcgtt ggacacggag cagaacaagt taaagcacaa ctaggagaac gcagtaaata
     1561 tgcgttacaa gcagaacaat tgggaacagg acatgctgtt ttgcaagctg cttctttctt
     1621 ggaaggaaaa aaaggaacaa cgttagttat cagtggcgat acaccattat tgactactga
     1681 aacgctgaac aatctttttg aatatcacca aggaaaaaat gcaagtgcaa cgatcttgac
     1741 ggcacaagca gaaaacccaa caggatatgg acgaatcatc cgtgaccata ttgggatcgt
     1801 agaaaaaatc gtggaacaaa aagatgcaac accagaagaa gctcttgtac aagaaatcaa
     1861 tacaggaact tactgttttg acaacgaagc tttgttcgat gcgttaagca aagttggaac
     1921 aaacaatgct caaggtgaat actatctaac agatatcatc gaaatcttaa aagaagaagg
     1981 ccatacagtt gctgcttatc aaacggatga ttttgaagaa tcaatgggag tcaatgatcg
     2041 gatcgcgtta gctaaagcaa atgagatcat gagaaagcga attaaccaga tgcatatggt
     2101 caatggcgta agctttgtcg attcagcaac gacatacatc gatgcaggcg tagaaatcgg
     2161 tccagacaca ttgatcgaag caggtgtaca aatccaagga aatacagtca ttggttctga
     2221 ttgtgtcatc gggtcacatt caaaaatcgt agacagtagg attgaggatc acgtcgtcat
     2281 tgaaaactca gtcattgaaa gcagccatgt caagaaacat gcagatgtgg gaccttatgc
     2341 acatttacga ccaaaagctg aaattggcga aaacgtccat atcggaaact ttgtggaagt
     2401 gaaaaatgct caaatcggca aaggaactaa agtcggtcac ttgacttatg ttggtgatgc
     2461 tacgctggga gaagaaatca atgtcggatg tggtgttgtc ttcgtcaact atgatggaaa
     2521 gaacaaacat catacgactg tcggcgacca cagctttatt ggttctagca cgaatatcat
     2581 cggacctgtc gaggtagcta aaaacagttc gattgctgcc gggtcaacaa ttacagacaa
     2641 tattccagaa tatgcgctgg caattgcgag agcaagacaa gtcaataaag aaggatatgc
     2701 aaaaaaactc ccttatctaa actaagactt ttgcaaaatc acttgattta tttaaaaaat
     2761 atgactacta tttattaggt agaattctag agttgtgaaa aagaaagtgg aggtccccat
     2821 gtcaaaacat tactttgacc caagattaaa aatttttgcg ttaaattcca atcgcccatt
     2881 agcagagaaa attgcagcag cagtcggcgt cgagttagga aaatcatctg ttactcaatt
     2941 tagtgatgga gaaattcaag taaacattga agaaagtatt cgtggatcgc atgtctatgt
     3001 tattcaatca acgagcagcc cagtcaatga taacttgatg gagttgctga tcatgattga
     3061 cgcactgaaa cgagcaagtg caaaaacaat caacgtagtc atgccttatt atggctatgc
     3121 gcgtcaagac cgtaaagcac gtgcacggga accaatcaca gcgaaattag tagcaaacat
     3181 gattcaaaaa gctggtgcaa caagaatgct gactttggac cttcatgcag tccaaatcca
     3241 aggtttcttt gatattccag tcgatcattt gatgggtgcg ccacttattg ccaattattt
     3301 cttagaaaaa ggaatcaaag gcgatgatgt tgtcgttgtt tcaccagacc atggtggtgt
     3361 cacacgggca cgtaaattag cggaattttt gaaatctccg attgcgatca tagacaaacg
     3421 ccgtccaaaa gcaaatgttg cagaagtaat gaatatcatt ggacaagtca aaggcaaaac
     3481 ttgtgtactg atcgatgata tgatcgatac agcaggtacg atcacattgg ctgccaatgc
     3541 tttgaaagaa gctggtgcaa ctagtgtgta tgcttcatgt acacatccag tattatccgg
     3601 ccctgcttta caaagaatag aagattcagc tatcgagcgt ctagttgtta cagactcgat
     3661 ctatcttcca gaagatcgta aaatcgacaa aatcgatgaa gtaagtgttg gcggactaat
     3721 gggagatgca atcaaacgta tccatgaaaa caaaccagtt agtccattgt ttgaaacaaa
     3781 aaataaataa taaataaact cacaaagggg agtccaattg ggctgagatt gaatctattt
     3841 ctaaaccctt cgtacctgta tcggttatgc gagcgtagga attgtgaata aacagctgtt
     3901 tgactatttt cgaaagctgc ttcctccttt gtgacttttt tattcaaagg aggaagtttt
     3961 tttatgggaa aaaatagagt ttgggtagaa ggaacgattg ttgcagcgtt agcgatggtg
     4021 ttgtcgttga ttccgatcca gatcggcagc agtttttcta tttcattagg gcagattcca
     4081 ttgactcttt ttgcgttaag aagagggaca aaggcaggga tgctggcagg tttcatttgg
     4141 ggcatcctac attttccgtt gggacaagtt tattatttaa gtgtcgttca agtgttgatt
     4201 gagtacccga ttgcctatac ttttgcaggc gcagcagggc tcatggcttc aaatgtccaa
     4261 aaaggctatg tagacgataa tacgaaacaa attagaaaaa gcatcgtgtt tggtgcattg
     4321 attggcgcac tttccagata tttttggcat tttattgcag gagtggtttt ctggggagcg
     4381 tatgcactat gggggatgaa tccttggctc ttttctttcg taatgaatgg tgccagcggg
     4441 ttgagtacag ctattgtaac gatcatcgtt ttattaggga tccaacaaac gactccttcg
     4501 atttttgtgc cagataaacg acaggagctg taaataaaaa agaagcaaga aacttgagac
     4561 agaatcatgt cctagtttct tgtttccttt ttattttttg ataagaaata gatctacttc
     4621 aatggcagat aagaaagatc ggcatcttct accaattgcc agtgtcgatc ggtgacagag
     4681 tagattaaag tagcgatgat ttcctcttct cgataccggg tgccttttaa tttccaaata
     4741 taatcttcaa tcacatagat tgggatacga tatttaccat cgatcaaacc aacttgatca
     4801 aaagaaaaaa tgatcccttc ctgcatcaaa ttacgcattt ctttcaataa ttccacactg
     4861 attttttcaa caggtgtttc ttttttgaaa aaagattttt ttacattgat cttatctgac
     4921 aagatcgaac ttaatttact agcaaaaatc ctataaaatt catctaaata gcgataataa
     4981 atccgattca gctgatcatt aagatcgaag tatacaaaat tattctgtag tttataaaaa
     5041 aacggcgaat gaagatgtgt tttcatatga ccaaaatata ataattctga aatttctaaa
     5101 ggtgtcaatt ctttcagcat cgttaaatca gtaaagtcga tccacttcaa ttcatcgaat
     5161 ccacgcctgt tacgagaaga ggaaaagaaa cgctgaatat tttctgtacc acggattact
     5221 ttcagccctg tatgggattc atattctcca gcagaagcag atggatcaag caagagcaag
     5281 ttttgaggaa aatgcacgat actgtttgca aagtcagtag cagtcagccc ttttgttaaa
     5341 acagcattac ttgtggtatc aatatgtaca taaactgact cagtcatcat gttcctcctc
     5401 ttatttcctc ttaatctctt ttattttaca acatttgcat taaaaaagct tgtttctttt
     5461 attttaaaat tccattacaa aatcacgttt tcattgaaaa cactctcatt ttcaattaat
     5521 ttttgtgatt tttgtatttt tttatttaaa ggaggtttta tgaattgttt tcttatggaa
     5581 tctacaaagt cgaatacgag actcatgacc tttttatgac gtactggtat acttgaagaa
     5641 aaaggagagc gcgatggaga taaaaaaagt ttacttttca cagaaaatgt cgctgtattc
     5701 gcctatccag caagcaaaac aaccccttcc attttttcaa agtcatcaag aaagtagaga
     5761 cgctttttgg cgttctgcaa aaaagcaatg tgcttttaaa gcagtatcga aaaaatatac
     5821 cgtctatttt actattgcta aaagtagagg agatgttatt tttaataatc tgttgattga
     5881 aggtgaaact tttgaatgga aaaaattttt tcggtatatg gaagcttgcg ctcgtttttt
     5941 tattaaagaa aactgttgtt ttttatttca atctcctcta tcagaagaat ggttgcgtat
     6001 ttttcataaa aacggctatc aaggaaccac acaattaaat aaaaagctgg tctatcatac
     6061 agcacttgtt ttaggtggag gaggagcgca cggtgcttat cagattggtg tgtggcaggc
     6121 gctgaaggaa catgatatca actttgagat tatcacaggt acctcggtag gtgcgttgaa
     6181 cggcgcattg attttacaag gagatatgaa aaaagctgtg aatttatgga aaaagctgtc
     6241 gacaagacaa gtgcttgcgt taccagaaat ggctgcagtt gaagaccttc gtgaacgctt
     6301 ctatcgagaa agacgccaaa tgacgaaaac agcactaatt gaaggcgggg tttcttctgc
     6361 tccattggaa aagatggtca aagacaattt ggatttgtcc ttactcaaac ataatcccaa
     6421 aatacgttta tatacggtgt ctactaaact gccagaacta gcggaagtag tgactgatat
     6481 ccaacagaca aaaacagaag aaataccaga ttggatactc gcctctgctt cattttatcc
     6541 cgctatggct tatcgaaaga tcggaaaaaa taaatatgca gacggtgggt atcgcaacaa
     6601 aatacctatt gatattgcga ttaacaaagg agcaacagag gcatttgtcg tagatgtgca
     6661 agggccaggc ccagcgaaaa aaattcgtat gccagacatt tttatccatt ggaaatgtca
     6721 aacattgtgg acactaggca gctttctgct ttttgatagc caaaggaacc aattaaattt
     6781 acaattaggc tatctggaaa tgaaaaaacg attaggctat tactatggga actggtatac
     6841 atttcattct gttaaacctg caagaacata ctggcgagga tttttgtctt acttaacaaa
     6901 agaaattcag ttagaattat cttttttcaa aagcataaaa ttttggcaaa aactgcgtaa
     6961 tttatataag aacagggtcg taccagagac atgcggattg gcgatgctag aacttttagc
     7021 aaaaaaacat tttttactgc ccaatgaaat ctatcaagta gacatgatga ttcaaatgat
     7081 ctgccaacaa gatataaaaa gtatgccgga tgatttattg gcacaaattg gtcaattaag
     7141 tactgaagaa tggcgaaggt atcgaaaata ccagcaaaaa atacaaaatg agcgcacgaa
     7201 aactgtttat tttgattatc tgctacaagg aaaacgcaag gatcgtttgc agcatgaatt
     7261 gcttaagcaa ccagttgaca cattactgat cctatattta tattatctca aggaggaaca
     7321 accatggcac aagaattttc atatgaaatc gtagaagaaa tcgctatatt gtcagaaaac
     7381 aacaaggggt ggcgtaaaga actgaaccta gtcagctgga atggtcgccc gccaaaattc
     7441 gatcttcgag attgggcgcc agaccatgaa aaaatgggga aaggcctgac attgacgaac
     7501 gaagagtttg aacaactgca aaaagcaata gaaaatatgt aatgaggaag gaccctgtcg
     7561 aatgaaaagt atgactggtt ttggcaaagc catccgcgaa acaaaagcgt atcagttaga
     7621 tattgaaata aaaagtgtca accagcgttt tctagatgtt caaatacgaa gtcctaaaat
     7681 cctgaacttt gttgaaaacg attttcgtca gcttattaaa cagcagttaa gccgcggtcg
     7741 aatcgaagta tttgtgaatc tagcttatat tggcaaaaac gaaaaacaga tcgtcatcaa
     7801 ctgggaattg attgattcac ttatcactca attgacagac ggaattcaac aacgatacgg
     7861 tagtcaaaat caagtgtcaa ttagtcggat ggcggaacgc ttcgtgctgg acgaaaactt
     7921 cgtaacggta gaagaaaaac cggaagagag catggaagaa ttggcacagt tagctctaga
     7981 tacattgaaa agcgcacttg attctattga ggcgagtcga gaaaaagaag gtcaagcttt
     8041 agctttagtg ttgaaaaaga atactgaaga attcaaagaa gtactaaaaa aactcgatag
     8101 ttttgtagaa atctatgaaa aggaatttca aataaggtat caaaaaaagc tggaggaatt
     8161 tcttggagcg aaagtcgatg agcaacggtt gttgacggag ctagccatct tgttggaacg
     8221 cggagatatt cacgaggaac tggatcgtct ggcgatccat gtacaaaaac tagatgaact
     8281 tatcgaagca caatgtcctg taggtcggga attagatttc ctgatccaag aaatgaatcg
     8341 ggaaatcaac acgatcggtt ccaaatcaaa tgcaatcgaa attaaaaatc aagtcgtcca
     8401 gtctaagacg atcttagaaa aaatcagaga acaagtacaa aatattgaat gacgaaagag
     8461 gtagagataa tgaaatatta tgacgtaacg ttccacgaat taagtggaaa aactgtggtt
     8521 aaacgggata tcccttctga aaaaaacggc tttgacgtat ggaaagatgc ctgtgcagac
     8581 tataatgaga acgaactgtt tattttgatc aacgacggtg cttacgtgac gatgaatcgg
     8641 aaatttattg ttcgaattga cacagaagag gtagaagatc cgacagaaaa agcacgttct
     8701 cgaaaagatg agatcatggg tgtagtgaat actttgtcca atatgggctt ttagcaagga
     8761 tgctgaacaa agaaatacaa taaataaaca gaattggatt tcttcgctta gcttctttgc
     8821 tctaggaata catttaaagg ttgtgacaaa attcgtgtca caatcttttt ttattttgaa
     8881 taagtgatgt tcaaaaacca ttttctcggt attaagccaa atttatgcaa aaatgtgaga
     8941 aactattttc acaaaatttt tcttcatact cgaaactaat cggcttcttc acgacctctt
     9001 atacacggcg ttcaaaagct tgcatctgcg ataataaccc caaccaaacg aaaaacatga
     9061 gaagctgttt atgtttggct gttcttatta cttgatgcag gacagctttt ttacgacctc
     9121 ttggtacagg gtagcttttt taccaatttc tagctataat taaagataac cttgaaagtt
     9181 gcagaaaaaa agtgcaaaat aaacgataag tacaaaaaga ctagttgaaa ggacgtcgtc
     9241 atgtcagagc gtggattatt aatcgtgttg tctggtccat ccggtgtagg aaaaggcaca
     9301 gtacgaaaag caatttttga gagtgaggat aatgattttc agtattcgat ctcaatgact
     9361 acccgtaaaa tgcgtgaagg ggaggtcgaa ggcgttgatt atttcttccg aagcaaagaa
     9421 gagtttgaat caatgatcga agctggagag atgctggaat acgcagaata tgtcgggaat
     9481 tattatggaa ctcctttgtc atatgtcaac aagacattgg atgaaggaaa agatgttttc
     9541 ttggagattg aagtccaagg agcaaaacaa gtcaaagaaa aagtgcccga tggtgtattc
     9601 atctttttga ctccgccaga tttagcggaa ttaaaatcac ggatcgtagg ccgaggaaca
     9661 gatgcagatg aagtaatcga aaaacgaatg aaagttgcaa aagaagaaat cgaaatgatg
     9721 gctctttacg actatgctgt tgtaaatgat gaagtgccgc tagcagttga acgaatcaaa
     9781 aacattattg ccagtgaaca ttttcgagta gatcgcgtaa tcggtaaata tattaaaatg
     9841 ttaaaggaga tgtagtctta tgatgttaaa accttcaatt gactcactat tagatcgtgt
     9901 aaattcaaaa tattctctag tgattttagc aagtaaacgc gcgcatgaat tggatgcaag
     9961 tgcacagcct actctagatt cttttgattc agtaaaaagc gtcggtcaag ctttagaaga
    10021 aatcgatgca ggaaatgtag tcaatgatcc acatcctgaa ttaaaacgtg aacgtttgaa
    10081 gatggaagaa gaggaacgtc aagcacaaaa agagcgtgaa caacacgaat tggaatcacg
    10141 tatccgtgaa gagcaaaaaa tgtaataaag aaaaatagag cgaacatcta aaaagaaaag
    10201 caaacccagc tgtttccttt tcaaacattc gccacttatt gtctggtaga aaaggctgtg
    10261 tctgaacttt ttgacacagt tctttttttt cttatctgct gttaattttt tgttaggatt
    10321 tggcagaaga taagacaaat ccatcgatct ctagtaaaat aggcaaggaa ctttttgtcg
    10381 cttgataaga aaagacaaaa aaaggaggaa acgaaccatg acattaacag ctgaagtcat
    10441 tgtggacgta cctactatgc agaccgatca gccattcact tatttggttc cctctgaaat
    10501 ggaaacagct ttgcaagtcg ggatgcgagt agaagtccct tttggtaatg gcaatcgtca
    10561 tgtccaagga tttgttatgg ctatccaaaa atcagaagaa tcagctaatc catctttgaa
    10621 ggcagttatt cgtttgctag accttgctcc ggtggtcaat gaagaattac tttcattagc
    10681 tgattatatg aaaaaaatta cttacgcatt caaaatcact tgtctacaaa cgatgcttcc
    10741 gagtgtgatg aaagcagaat atgataaatt gatctatcct ttggcagaca caccagaagt
    10801 tcaggtattg tttgatcagc gagaagtgat ttcctggaag gaagcagaag aagaaggcag
    10861 cctttctcaa ttgattcgct ggcgccaaga gcaattagta gatatcaaat atgaagtcca
    10921 tacacgaaat aaagtaaaaa cgattcgttt ggtccgttct ttgttaactg aaaagcaaat
    10981 cgaagaagaa tgggcaaaat tacggcaaaa tgccaagaaa caaaaagaac ttcttttgtg
    11041 tttatctgaa ctgagccaag aagaaccaat tgcttatttc aaaaataaag gaatcagtac
    11101 tgctgtcttg aatcaaggaa aagaaaaggg ctggctggag tttatagaat cagaacgtta
    11161 tcgcgaccct tataaagacc gagtatttga ccaaacgacg gcattggaac tgaatgcaga
    11221 acaaaaaaat gctgtcgaac aaatcatcac agcaggtcag cagcaaaaag atcaggtctt
    11281 tttgctggag ggaatcactg gcagcgggaa aacagaagta tatcttcagg caatcgctga
    11341 tgttttgtca gaaaataaaa cggctattat gttagtgcct gaaatcgcat tgactccgca
    11401 aatggtcgag cgattcaaag gacgcttcgg agaatcagtt gcggtcttac atagcggact
    11461 ttcccaaggt gaaaaatatg atgaatggcg caagattgag cgggaagaag cgcaagtagt
    11521 agtaggcgca cgatctgcta tttttgcccc gctgaaaaat atcgggctga tcattatcga
    11581 tgaagaacat gaatcaagtt acaaacaaga tgagacaccg cgttatcatg cgagagatct
    11641 agcaatttgg cgcagtaaat atcatcattg cccaatagtt ctaggaagcg cgacgccctc
    11701 tttagaatca agagcacgag ctcaaaaagg cgtttatcag ttattgcagt taaaccaccg
    11761 agccaaagca gctgcacagc ttccagctat tgagatcgta gatatgcgtg aagagtttca
    11821 gaatcatcgg acctctactt tttctgctaa tttgcaggaa aaaatacaaa atcgattaga
    11881 caaaaaagaa caaactgtcc tactgctcaa tcgtcgagga tattcgtctt ttgtgatgtg
    11941 tagggactgt ggatttgttc ttccatgtcc taattgcgat atctctctta cactgcatat
    12001 ggatacacgt tcaatgcgct gccattattg cggacatgaa gaacctattc ccaatcgctg
    12061 tcctaattgt ggcggcaata aaatccgcta ctatggaaca ggtactcaaa aagtagaaga
    12121 ggaactccga gaactttatc cgcaagctag gattttgcgt atggatgtag ataccactcg
    12181 aagaaaaggg gcacacgaac agattttaca aaaattcggt gcaaaagaag cagatatatt
    12241 attagggaca caaatgattg ctaaaggttt ggactttcca gaagtgacat tagttggggt
    12301 gttgaatgct gatacctccc tgaatttacc ggattttcgt tctagtgagc atacattcca
    12361 attgcttacc caagtttcag gacgagcagg gcgagcggaa aaagcaggcg aagtcgtgat
    12421 ccaaacattc aatcctcaac attacgccat agaattggct aaaaaacaaa attatgagca
    12481 gttttatcag caggaaatgc atgtacggca tcgtggaggc tatcctcctt attactttac
    12541 tgtgaaaata accgccagcc atcctgaaga acaagtagcg gcaaagaaaa tctttcagat
    12601 tgccaatcag ctaaaagaag tgttaagccc tcaaagcttg ctattagggc caacgcccag
    12661 catgattttg agagtgaaaa accgatatta ttatcaacta atcatcaaat ataaacatga
    12721 accgaatctt cctcatgttt tggatgaaat tttgaatgga agccagaaag aacagcgaca
    12781 aggtcttttt gtggcaattg acaacgaacc attaaacttt atttaagaaa gggccaaatt
    12841 atgcgctatc ctatattgat ccaccctaat gataaattaa aaagaactgc tcagccaatc
    12901 gatgtaatca cggacgagac aatcgcttta ttagacaatt tatatgagac aatgattgcg
    12961 aatgacggaa tagggatcgc tgctcctcaa gtaggacaaa acaagagaat tgctgtgatc
    13021 gaagtagatg aaggcgagaa atttgaactg attaacccag aaatcatcga agcaaaaggt
    13081 gaaagccttg acgtagaagg ttgtctcagt attccgcatg tctatggaac ggtcaagcga
    13141 gcagatgaag tgaccgtgcg ttattatgat cgagacggag aagagataga agtgacggct
    13201 tttggctatt tagcccgtgc cttccagcat gaaatcgatc acttagacgg tatcttgttc
    13261 attgagaaaa tgatacagca gatcccggaa gaagaacttg aagaatatat ggaggaacat
    13321 cttgatgact aagctagtat ttatgggcac acctgctttt tctgtgccga ttcttgaagg
    13381 gctgatagaa tcaggttacg agatccaggc agtggtaaca cagcccgacc gtccagttgg
    13441 gcggaaacga gtaattacgc cgacaccggt aaaaaaagcg gctctgaagc acggtatccg
    13501 tgtgctgcag ccagagaaaa tctccggttc gccagaaatg gaagaaatca ttgaattggt
    13561 gcctgatgtg atcgtcactg cggcatttgg ccaattcttg cctgaaaagt tattgcaagt
    13621 accaaaatta ggggcaatca atgtccatgc ttctttactt cctaaatatc ggggaggtgc
    13681 gcctgttcat tatgccatca tgaatgggga gaaagaaact ggtgtaacga tcatggaaat
    13741 gatcaaaaaa atggatgcag gcggaatcta tgcgcaagaa agcataccaa tcactaaaca
    13801 agatgatgta gggacgatgt ttgaaaagct tagcctttta ggaagaaagc tattgctaga
    13861 aacattgccc aatattttgg atggacaaaa accagtacct caagacgagt cagaagcaac
    13921 tttttcgcca aacatcacac gggaacaaga agcaatcgat tggcataaaa ctgcagaaga
    13981 aattgacaac caagtacgcg gaatgcgtcc ttggccaatt gcattcacga cctatgaggg
    14041 catacgctgg aaattattat cggttgaagt attggcagaa actacagaca aagcacctgg
    14101 tacgatcatc cggaaagaca aaaaaaatct atggattgct tgcggtaaac agaccgtctt
    14161 agccatcaat gaattacagc cagctggtaa aggaaaacag gccgtacatg atttccttaa
    14221 cggaagcggt cagcaagttc agattggtca gcaggtgaaa taatgaaaaa aattcctaaa
    14281 aaaatgaaaa aatcagtacg atatatggca ttactgacat tagagcgagt gaacaagggt
    14341 ggcgcctact cgaatctttt gttgaatgag atgatcaaac aaagcgagct aaatcaaaaa
    14401 gatgtgggtt tatttacaga attgatctat ggtacgatta gccatcagcg actattggaa
    14461 ttttacgttg caccattgat cgctaaagca aaaaaagtgg atgactgggt caagatgctt
    14521 ctttatcttt ctgtatacca attagaattt ttagataaag tccctgcaca tgctatttta
    14581 aacgaggcgg tcgaaatcgc aaaagtaaaa ggaaatcctg ggacagggaa atttgtaaat
    14641 ggtgtcttga ggaattatca gcgtcaagga gcaccagatc tgcaaggcat tgctgatcct
    14701 atcgaaagac tttctgtcaa aatcagcctg cctctatgga tgacgaaacg actggtggat
    14761 caaatcggat acgaagaaac tgaaaagctc ggtttatcgc tatatcagcc tagccatgct
    14821 agtgcccgag tagatacacg ccgactttcc agagatcagg ccatcactgt tttgcaagaa
    14881 gaagagatca cagctgaaaa aagccaagtc agtccatacg gtatagtagc gaaaaaaggg
    14941 catttagctt ctagctcatt gttccacgat ggcgtcatga cgatccaaga tgaaagttcg
    15001 atgcttgtag cgccgtctat gcaaatcgag cctgaacatc atgtcttaga tgcctgtgca
    15061 gcacccggag gtaagacgac gcatattgct tcttttttag atccgtcatg cggtggagaa
    15121 gtggtcgctt tagatgtaca cgaacacaaa gtcaaactca ttgaagataa tgcagcacgt
    15181 ctaggtgtct cttctgctgt ctacgctcaa aagatggatg ctagagaagt cagtaaacag
    15241 ttcgaagcag agcagtttga tcggatttta gtagacgcgc cttgttcagg tcttgggctg
    15301 cttcggcgaa aaccagatat tcgttataaa aaacagccgg atgaattagc gaatttacca
    15361 aaaatccaat tggaaatttt agaaagctgc gcaccaactt taaagtcttc cggtattctg
    15421 acttatagca cttgtacgat cttaaaagaa gaaaaccaag acgttgtttc tgctttcttg
    15481 gaaagacacc ctgattttga gaggattgat gttttgacca atcctatgct acaatcatca
    15541 atcgaagaga aaatgttgac tttgtatccg catcagtttt acacagatgg atttttcatt
    15601 tgttgtttgc gcaaaaagta atgaggtgaa aaaatgcaga ttgaatatca atcagatgta
    15661 ggaagacgac gtaacacaaa tcaagactat gccagtgtat ttaccagccg ttcaggtctc
    15721 aagcttgctg tattagcaga cgggatgggt ggtcatcgtg ccggagacat tgccagtcag
    15781 atggcggtaa cgaatctagg cgaagcttgg gaaaaacaag aagctgttga cgatgaaaaa
    15841 atcgcccaat ggttcattca atcgattcaa gaagaaaata tgctgattta tcagcgtgga
    15901 caagaacagc cggaatataa cggtatggga acaacaatcg tagcggccgc tctgtcagat
    15961 gactggttca cgattgccaa tgttggggat agcagagctt atttgattcg tgaagataaa
    16021 atcatgcaat tgacggagga tcattcttta gtcaatgaac tagtcaaatc aggagaaatc
    16081 agcgaagaaa tggcagtgaa ccatccaagg aaaaatattt taacacgttc tgtcggcatg
    16141 cctggaacag tcgaagtaga tgtttctact catctttggc aactaaaaga tcgactgctg
    16201 ttatgttcag atggcttgac gaacatgctg tctgaagaag aaataggaac gatcatcaat
    16261 caagaaggcc cattgtctca taaagtaact gagctgattg atcgtgccaa tgaagcaggc
    16321 ggagcggata atatcaccgt tcttcttatc gaatataagg aggacagtgt atgatcgagt
    16381 tgggcaaaaa attaaatggc cgttatcaaa tcactggaaa tatcggtagc ggtggtatgg
    16441 caaatgtatt tttggcgcac gatttaatct tggatcgtga tgtagcagtt aaagtcttac
    16501 gatttgactt tcaaaatgac cagactgcga ttcgacgatt tcagagagaa gctttagcag
    16561 caacagaact cgttcatcca aacattgtta gtgtttacga tgttggtgaa gaagacaaca
    16621 tgcagtatct ggtgatggaa tacgtcaaag gaatggactt gaaacgatat atccaaacac
    16681 attatcctgt tccttatgaa acagcagtaa atattatgca gcagatcctt tctgctattt
    16741 ctttagctca taaccatcaa atcatccacc gagatttgaa accccaaaac gtactgatcg
    16801 ataatgaagg tgttgtaaaa atcacggatt tcgggattgc tatcgcttta tctgaaacat
    16861 cgatcacgca gacgaatacg atgctaggat ctgttcatta tctgtcccct gagcaggcca
    16921 gaggaagtat ggcaacgaag cagtcagata tttatgcttt agggatcatc ctttatgaga
    16981 tgttgacggg tagcgtaccg tttgatggtg aatctgctgt aacaattgct ttaaaacatt
    17041 ttcaagatga tcttccatct ataaaagcac ttgatccaaa tgtgccacaa gcactggaaa
    17101 acgtgatttt acgggcaacg gctaaagaac ctgcagaccg ttataagtca gccgaggaaa
    17161 tgtctgatga tctttctaca gtattatcac cagctcgtgc aaacgaagaa aaatggcagc
    17221 ctcatgtgat ggataatgaa acaaaagtta tcacacccct tacggaagat gcaccaatgc
    17281 ctgattcatt caaatctatg cctctgccaa aagacaagcc agaaaacgaa accttaccag
    17341 aggaggaaga agttctgcct gatcctaaaa agaaaggaca gaaaaaatgg tggctgatcc
    17401 tattagctat tcttgctgtt ttaggaatcg gaacagctgt attctttgct tctggcgggc
    17461 gaggagaagt tgttgttcct gatgtttctg atctttctga agcttctgct agagaaacac
    17521 tcgcaaaagc gaaattagaa gtagcaacaa aaacagaaga aattgcagat gacaaaatcg
    17581 aagaaggtaa tgtagtgaag acagacccgg cagcgggaac gacagtcaag caaaaacgcg
    17641 aagtcactct gtatattagt agcggcacga aaaaaatcaa attagatgat tacagtggta
    17701 aaagttatga agatgccgaa gcaagtttga aagaattagg gttttctgct gacttgattc
    17761 aaaaagaaga ggaattcagt tcagaggtag aaagcggaat gatcatttcg caatctccat
    17821 cagaagatac agaagtcgat cctaaaacag acacgattac tttcacagtc agcaaaggac
    17881 cagaaacagt gactttgagt agtcatgtag gagaaaacta cgaaactgtt cgtaattggc
    17941 ttgttacaca aggattcaat atcaataata ttagtgctac ttatgactat agcgacactg
    18001 taccatcagg ttcgatcatc agtcaggatc cagcctcggg tgaagttgta gcagaagaaa
    18061 cttacatcaa ttttgtcgta agccaaggaa cagagcctaa attgaatgat atctccggct
    18121 atacaaaaag cgaagcacaa agttatttag ctagtgtcgg tgcagaatat atcgggcatg
    18181 agacttccga atattcaaac acggtggata aagataaagt gattcgtaca gtaccagggg
    18241 caggaacgac aattaccaaa ggaatggttg tgaacgtaat ttactctaaa ggaccagatc
    18301 cagcgtcttc tagttctgaa aaagactctt cagacagttc tagcagctca agtagctcca
    18361 gtagcacaag caactcttca aaagaagaat cttccagctc cacaagttca tcagctagca
    18421 cctcagaaag ttcaaaataa gtgctgaata aaatatatca gaagggaatg cgacattgat
    18481 ttgttgcatt ctcttttttg tgtttctatc tcttttgatc aattttcaag ccatatactt
    18541 tgagaagttt gcatggaata acacctactt tcctcttcgt atggtaaaat aaacaaaaac
    18601 ggaggtggca cttatcaaag gacaaatcag aaaagcaatc agcggttttt actatgtata
    18661 cgcaaacgga gagacctatc aaacaagagg aagaggaaat ttccggaatc ggaaaatcac
    18721 gccgcttgtc ggagatcagg tgatctttga gagtgaaaat caaacagacg gctatcttct
    18781 ggaaatcttg ccgagaaaaa atcaattagt tcgcccacca gtcacaaatg ttgatcttgg
    18841 aatcattgtc acaagtatcg tagagcctgc attttcttat aatctacttg accgctttct
    18901 agttaccttg gaatacgagt cgatcgaacc gattattttt ttgacaaaga ccgacttgtc
    18961 agacgaccca gaaaaaatcg aagagatcaa gcaaacctat actaaaatag gctatccggt
    19021 gattgtaccc aatcatacgg gtgatacgac agaactgatc cgttacttcc cagaacggct
    19081 atcggtcttt atgggtcagt ctggagcagg aaaatcgacg ctactgaatc aaatatcacc
    19141 ggaactgaat ttagcaacag gagagatttc tgatgcactt ggaagaggac ggcatacaac
    19201 acgtcatgtg gaactgattc ctatctatgg cgggttagta gcagatacac caggatttag
    19261 ctcgattgac tttttgacaa ttgaagcagg agaactgccg agacaatttc ctgaatttgt
    19321 agaagcttct gtccattgca aatttagaga gtgcatgcat gctaaagaac ctggctgcga
    19381 agtaaaacat agagtagaaa caggtggtat cgcacagaca cgttacgaca attatttgca
    19441 attcttacaa gaaatcgaaa aaagaaaacc aatgtatgca aaaaaagaag gtaaacgaaa
    19501 ataggaggaa acaagaatga agatagcacc atccattttg agtgcagatt ttgccaattt
    19561 acaaagagac attgagttag tggaaaaagg aggcgcagat tacatccacg tagatgtaat
    19621 ggatggccga tttgtcccaa atatcacatt agggccaaat attgttcagg caatccgtcc
    19681 tgttactaaa ttaccgttag acgtccatct gatgatcgtt gatccagaaa actacattcc
    19741 agcatttgca aaagctggag cagatatcat tactgtccac gtcgaagcaa ctcctcatat
    19801 tcatcgggca cttcaaatga caaaaagctt aggagttaaa gctggagtag ttatcaatcc
    19861 aggaacacca gtctccatga tcaaacatgt tttgcctttg gctgatcaag tactagtcat
    19921 gactgtcaat cctgggttcg gcggacaaag ctttattgaa gaaacagtag aaaaaattac
    19981 ggaactcgct gatctacgta gagagaacaa ttggacttat gcaatcgaag tagatggcgg
    20041 aatcgttcca gaaacagcaa aaatctgtaa agatgcaggg gcggatgtct tcgttgcagg
    20101 atcatatatc tatggagccg aaaatccagt cggacaaatc gaacgattaa aagaggcatt
    20161 agactaaatg aaaattttat tagctgctgg aggtaatccg caaaactggc cgcagtctct
    20221 gctgtccaaa tacgatgcct atgtcgggat agaccgtggt gcgctgtacc tgcttcaagc
    20281 aggctaccca gtagatctgg cagtgggtga ttttgattca ttgtcgaacg aagagcagaa
    20341 ggaagtattt gcaaaagcca aagagtatac ccgttcccct gcagaaaaag acgatacaga
    20401 tacacagtta ggtatcttaa aaacatttga acgttacccg caagcggaag tcactttgat
    20461 tggagctacg ggcggaagat tggatcatct tttagccaat ctctggttag gtatggaacc
    20521 tcgcttcaaa ccctttgccc atcagcttat tatcagagat tgtcaaaatt atttgtctta
    20581 ttatttgcct ggagagcata tagtaagaca gataccagat atgaagtatt tagcttattg
    20641 ctgcttgact cccgtctcga atcttacatt aaaaggcagc aagtatgtat tggaaaataa
    20701 agaagtagaa tatccgacat cttatgcaag taacgaattt ctttcagata ccgcagaatt
    20761 ttctttttca gatggtatga ttgcagttat tcaaagcaaa gattgatcca taaacaaata
    20821 aaaaaatttg tctttcaggc taaaaatgac agaatctatt atttgctaaa aaaaagctta
    20881 agaggagcaa cttacctttt gtttcacggc atttagtgat aaaattgaac acaagagtct
    20941 aattaattcg aggtgtcttt gtgaagaagt tcaatcctaa taaaaatatt attattacct
    21001 tggttattgt tatcatcgtg gtgacgatac taagtgtgac cgctgcgcag cgagcgaatg
    21061 aaggaaagcc caatttcttc caatccatcg ttaatgacag tgttgcaatg gttgataaaa
    21121 caatctctgc cccagtacgg tgggtacgaa atggagtaga ttctgtccat aatctttttt
    21181 caacttatgc cgaaaatgag cggctaaaag aaaaaatcga tagctatgat gacgtttcac
    21241 agcaaaacaa gaattacgag agagaaatcg aagcgttgaa aaaagaactt gagttgaatc
    21301 agacgctgac tagttacgaa aaagtaacgg caaatgtcat tactcgttcg cctgacagtt
    21361 ggcaggacat gctgatcgta gacaagggtt ctaaagacgg tatcgaagca aatatggcag
    21421 tcatgtcgca aaaaggattg attggccgag tgatcgaggt caatacggct tcgtctaaaa
    21481 tcgaattact gtcatcctct aatgaaagct ccaatcattt tccagtacgg gtatcttcgg
    21541 ctaatggcga agcgtttggt ttgcttaaaa actatgatga aaagctccat gccttagtgg
    21601 tgacccaatt aactggtgat acggatatca aagaagggga tgttgtccag acatccggtc
    21661 ttggagggaa ttctccagct aacttgccga tcggtacggt tattaaaacg aaaccagata
    21721 gttatgggct ggatcgggaa gtttatgtga aaccttatgc agaaatgtat gacgtgtcag
    21781 ttgtgacgat tgtccaacgg ctagcggagg attagtccat gttgaagaaa gaaacaatga
    21841 agtactatct gccaatcgtt ttgttctttt tgatgttgat agatggtcat ttaacaagaa
    21901 tgctagggga gtggtcgaaa ggcacctata tgtcaaatgc ccactttctg atattggcat
    21961 tattatgttg cagtatggcg tttgaaaaac gttatttact gattaccacg attgttctcg
    22021 gggctatcta tgacgcttac tatattggcg ttatcggtat ctatgcagta gctctccctt
    22081 taattgtatg gttgatgtat gtaatgaaag acgttatcca tgtcaacatc tttactgaat
    22141 ttttcagtat gatcatcttt gtcacgggtt atgaattgtt tacgatggtg gtccagttga
    22201 tttttaaatt agcagtagta aataacacgt attttattac aaggttttta ggacctacac
    22261 tgctgttgaa catgattata tttgtattat tcattt
//
LOCUS       DAXLPI010000040        22275 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXLPI010000040 DAXLPI010000000
VERSION     DAXLPI010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.040"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..1132
                     /locus_tag="ACF3T5_001296"
     CDS             <1..1132
                     /locus_tag="ACF3T5_001296"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD3631919.1"
                     /translation="FTDVSAAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSII
                     TSMVMTYMFSFEGIKGYGYVVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIF
                     MIYLLTIGLFVSYVAHQYQVFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQL
                     KRKELVYLKKYTDSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIY
                     SNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDC
                     IRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGIT
                     TKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            1147..1899
                     /locus_tag="ACF3T5_001297"
     CDS             1147..1899
                     /locus_tag="ACF3T5_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD3631920.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2602..2817)
                     /locus_tag="ACF3T5_001298"
     CDS             complement(2602..2817)
                     /locus_tag="ACF3T5_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HFD3631921.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2836..3072)
                     /locus_tag="ACF3T5_001299"
     CDS             complement(2836..3072)
                     /locus_tag="ACF3T5_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFD3631922.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            3330..5483
                     /locus_tag="ACF3T5_001300"
     CDS             3330..5483
                     /locus_tag="ACF3T5_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HFD3631923.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5452..6867
                     /locus_tag="ACF3T5_001301"
     CDS             5452..6867
                     /locus_tag="ACF3T5_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFD3631924.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6900..7934
                     /gene="sppA"
                     /locus_tag="ACF3T5_001302"
     CDS             6900..7934
                     /gene="sppA"
                     /locus_tag="ACF3T5_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFD3631925.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7936..8457
                     /locus_tag="ACF3T5_001303"
     CDS             7936..8457
                     /locus_tag="ACF3T5_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFD3631926.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            8454..8840
                     /locus_tag="ACF3T5_001304"
     CDS             8454..8840
                     /locus_tag="ACF3T5_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFD3631927.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(9336..9408)
                     /locus_tag="ACF3T5_001305"
     tRNA            complement(9336..9408)
                     /locus_tag="ACF3T5_001305"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9373..9375),aa:Lys,seq:ctt)
     gene            9789..10358
                     /locus_tag="ACF3T5_001306"
     CDS             9789..10358
                     /locus_tag="ACF3T5_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFD3631928.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            10345..11205
                     /locus_tag="ACF3T5_001307"
     CDS             10345..11205
                     /locus_tag="ACF3T5_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFD3631929.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            11395..12099
                     /gene="yycF"
                     /locus_tag="ACF3T5_001308"
     CDS             11395..12099
                     /gene="yycF"
                     /locus_tag="ACF3T5_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFD3631930.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            12104..13939
                     /gene="walK"
                     /locus_tag="ACF3T5_001309"
     CDS             12104..13939
                     /gene="walK"
                     /locus_tag="ACF3T5_001309"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFD3631931.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            13936..15252
                     /gene="yycH"
                     /locus_tag="ACF3T5_001310"
     CDS             13936..15252
                     /gene="yycH"
                     /locus_tag="ACF3T5_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFD3631932.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            15253..16122
                     /locus_tag="ACF3T5_001311"
     CDS             15253..16122
                     /locus_tag="ACF3T5_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFD3631933.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            16177..16986
                     /locus_tag="ACF3T5_001312"
     CDS             16177..16986
                     /locus_tag="ACF3T5_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD3631934.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(17089..17718)
                     /locus_tag="ACF3T5_001313"
     CDS             complement(17089..17718)
                     /locus_tag="ACF3T5_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD3631935.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            17888..19180
                     /locus_tag="ACF3T5_001314"
     CDS             17888..19180
                     /locus_tag="ACF3T5_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFD3631936.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(19256..19918)
                     /locus_tag="ACF3T5_001315"
     CDS             complement(19256..19918)
                     /locus_tag="ACF3T5_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFD3631937.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            20143..20427
                     /gene="groES"
                     /locus_tag="ACF3T5_001316"
     CDS             20143..20427
                     /gene="groES"
                     /locus_tag="ACF3T5_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFD3631938.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            20478..22103
                     /gene="groL"
                     /locus_tag="ACF3T5_001317"
     CDS             20478..22103
                     /gene="groL"
                     /locus_tag="ACF3T5_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFD3631939.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         7624 a         3555 c         4446 g         6650 t
ORIGIN      
        1 atttactgat gtttccgcag ctatacctat aatcggaagt tattttatat taaaggaaaa
       61 gaagaaaact aatatagtgc ttttaaataa attactatta tgtatgctta ttgatgattt
      121 cagatctatt attacttcca tggttatgac gtatatgttt tcatttgaag ggataaaagg
      181 gtatggttat gtagtattcc agataatgct tgattctttg tttttgctga tgtttatttg
      241 gttatataga aaattagcta taaataggat tgttgaacag tccagttcta aacttactgc
      301 tatattcatg atttatttac ttacaattgg tttatttgtt tcttatgttg ctcatcaata
      361 tcaagtattt gatcattttg tgtttggtgt cttaatattt ttaatcattc aaatgtttgt
      421 tgttctattt ttatttatta gaataactat taggcaacga gaacaatatg aacagcaatt
      481 gaaaagaaaa gaattggttt atctaaaaaa atatactgat tcactggaaa aagatcaaga
      541 aaaatttgcc agatttagac atgactataa aaatttattg ctgagtttaa aagaaatcgc
      601 taatcaaaac catgattcga tattggaaaa agaaataaaa gaattagaga tctattctaa
      661 ttcttatcta accaataagt ttgaatatcg ttatttaaaa aatataaaaa atgaatattt
      721 gaaaagtttg ttgatagcga agctatacca agcaaataac aataagattt gttgtaaatt
      781 cgaatgtcca agtactatta ttgaagttcc tgttccaata tttgactgta ttagggtgct
      841 tggaattatt ctcgataatg caattgaagc atctatagag agtcaagata gagaactgtc
      901 tctagctatt taccgagatg ataaacagat agaaatttcg atatcgaata gttgtagaga
      961 gatactactc tctatagata ctctaatgaa aaaaggaata acaacaaaaa agggacatca
     1021 agggttaggt ttatctagta tagaggaaat caataaaaag aatcccaata tgtttgtgaa
     1081 ctatcaaaac gatccgtatc aatttactac tcaagttatt ttgttgtttt gagggaagga
     1141 ggatttatga gttatccaat aatattatgt gaagatcaaa ttatacaatt aaatcagtta
     1201 gaacgaatta ttgataattt cattcttttt cacgataaag tatttaagat agtattgaaa
     1261 acacaaagtc cgttagaagt taaaaaatac ctcaaacaat tccgaccaaa gcaaggaata
     1321 tatttcttag atattgattt aaatcatgaa gttaacggta tagaattagc agaagtaatc
     1381 agaaaatatg atgttcaagc aaaaatcatt tttacaacta ctcatgatga gatgttaccc
     1441 gtaacaataa aaagaagagt tgaaacgtta ggatttgtaa caaaagatca aacactagat
     1501 gagtatcgaa acgagattgt tgagttattg ttattagcgc aagaaaggat agatgcaaca
     1561 aaacaggtta cgaatcaagc atttgttttt tccataggtt ctcaaacatt tacttttgat
     1621 ataaatgaca tttattttgt agaagcttca aaattaccac atagattatc attgtgtact
     1681 aaagatgggc agtacgaatt ttatggaaga attagtgagc ttgaaaagaa atatcctatg
     1741 ttaacaagaa ttagtcgagc gtgtttagta aatatcttta acgttaaaga aatagatttt
     1801 aaaaaacgtt cactatattt tgattcagag ttggccagaa actttacttt agggaaagca
     1861 caaaaaatta aggagaagtt gaaagaaagc actgtttagt ggttttttct ttagggagat
     1921 tgcatttttc taataaaata gattccttca ctagtgcaaa tttttctgaa tcctattaaa
     1981 gttgtaccaa tttttatagc tgttattagt tacgtgcata acagcaatat tagttactgg
     2041 aggaatattt ctatggtctg gaaccaaaaa agggccaaat gaatagttct tttcttgttt
     2101 ttattaagta ccataaaggg gaattaaaat taaatgtatg gtggaagatg cgaaagattt
     2161 ggcaaattct actaattttt agaaagtgtt tttcattttt tagttaaaaa atttgtcctg
     2221 tcaagtgcaa tcctatgatt gtacttgata ggatttgaga taattacttt cctgttttta
     2281 taaatttaac ttgcaggaac aggctttaat gcacatccga caagtacatt accaatacct
     2341 cctgtaacgg ccgaaatagg tcctcctgca gcaccaacta cagcgctgca agtccttttg
     2401 taaaaatatc tctaccatta catttccaaa gaatttttat ctgactttaa taaaagataa
     2461 aaatttaata taattataaa aactagcaca agaagcttta gtaaaaagtt ctgtccattc
     2521 caaacttttg aaaagataaa gaacatacct atgttcgtaa tcgacacaaa ccaatcttga
     2581 ggtttgatat tttttaattg tttacgcacc ggtccacatg ctgaatccag cacctaataa
     2641 agctccacct acaccacaag cagctattgc ataaggtcct ccggctgaac aaagttttac
     2701 tcctgctgtt gccccagaaa aagtagtgcc taaaactccc acggcactcc caccttgaat
     2761 atttttcaac tcttctgaag ataatataaa tgttgttttt gtgtttttaa tatgcatact
     2821 acttattctc cttttttatt tttttcgagc atcccagcca gctgcagcac ctccagcgac
     2881 agctccatat aaccctccaa caacaccgca agctggtccg caaattgctg ttcctaaacc
     2941 tgcaccacct actgcccctg tggtaagtcc acctacaact tgccccaatt ttccccaagc
     3001 attattgcct ccaccaacag tttgtgcatt tccacaatac ttagttcctt tacattttgc
     3061 atgtttaaac acgcttcttt aaataatatt ttttacggtg ctactgtaac atacgtattt
     3121 gattaacagt aaaaatcttc ctgaaactta tcaagtttat tcttgaatag taaaatttcc
     3181 aaaataaatt tgattcagga atgaaaaagt tagtgttcaa gaactgtttt caaagttttt
     3241 gtggaattat gagacaatat tttcactgta ttaaagctat attgagaatt tttctataaa
     3301 aattttttag aaggagttgt gttagataag tgataaaatc atttcctttt gtgcagcaac
     3361 aagatgaaaa agattgtggt gttgcttgcc tttcgatgat tttaaaatat tttaaaactg
     3421 atatcccgat tcataaatta cgggacctat ctgggactga tttagagggt acttcagcat
     3481 ttggattgaa aaagacattt gaaaaattgt cttttgattg tatggctatt caagcagata
     3541 gtgagatctg gaaagaaaga gatcttatct taccattaat tgcacatata ttggttgata
     3601 acagctatat gcactacgtt gtaatttata aaacgaaggg aaacgtacta tggattgcag
     3661 atcccgctaa tggaaaaaat agaaaaacga ttgaagcgtt tcaaaaagaa tggacaggta
     3721 ttcttttatt acctatgcct agagaaactt atattcctat taaagaaaaa gtagctggat
     3781 taagttcttt tttgcctata atttggaaac aaaaaggatt agtattccac atcgttttag
     3841 catcgatttt cattacgttt tttggcatag gaagctcata ttattttcaa ggtattcttg
     3901 attttttcat ccctaatcaa gcacgttcaa ctttaaatat aatttccatt ggcttgatcg
     3961 ctgtctattt atttcgggta ttgtttgaat atagtcgtag ttatcttctt attattttag
     4021 gacagcgaat gagtatggca attatgcttc agtatttcaa acatgtgtta actttaccaa
     4081 tgaatttttt tgctacgaga aaatcagggg aaattatttc tcgttttctt gatgccagta
     4141 agattattga tgctttagca agtgctacgt tatcaatatt tttagatatt ggtatggtgt
     4201 tgcttgttgg ggttacgtta gctattcaga atagtacgtt gtttttaatc actctatgct
     4261 ctttgccttt ttatgtagtt gcaatattcg ctttcgtgaa aagctatgaa aaatcgaaca
     4321 gggaagaaat ggcagccggt gcagctgtaa attctagtat catagaaagt cttaaaggaa
     4381 tagaaacaat taaagcatat agcggtgaag agaaagtata taaccgtata gaccatgaat
     4441 ttgttaaatt aatgaaaaaa tcatttcgta cggttacgct tgataatatt caacaaggaa
     4501 ttaaacattt gattcaatta attagcagtg cattaatttt atggctaggt tcttattttg
     4561 ttatggataa tagaattagt ctaggtcaac ttatcacata caatgcttta ctagtatttt
     4621 tgacagatcc tttacaaaac attattaatt tacaagtgaa aatgcaaacg gctcaagttg
     4681 caaataatcg tttaaatgaa attttcgcga ttgattcaga aaaatcgaac cagtcttttg
     4741 aatatatgct ttctgaacaa ttatttcaac aagatatcac tgtaaaaaac gtttcattct
     4801 cgtatagcat gaaggcccca acgttgaacg aaatttcatg tacaattcct tcgcacagta
     4861 aaacggctat agttggagtt agtggttctg gaaaatcaac tcttgcaaag ttatttgttc
     4921 gtttttatga agcatcagaa ggatcgatcc attatggaaa cattaatcac ttagacattc
     4981 cttttcagat attacgcgat cgagtgacat atttgcctca agagtcattt ttctttagtg
     5041 gaacaatcat tgagaatctt atattcggtt tagatagccc accaagtttc gaacaaataa
     5101 tagaagtttg tgagatagtc caactaaatg aatttattaa tcaacagcca ttacgttacg
     5161 agacattgtt ggaagaaggt ggaaataatc tttcaggagg gcaacgacaa cgattagcct
     5221 tagcacgggc tttattaaaa aatccggaca tattaatttt agatgaagca actagtggat
     5281 tagatacctt gttagaacat gcaattatga attatctcct caatttaaaa gaaaaaacga
     5341 ttatttttat tgcgcatcat ttaccaatag caaaagcatg tgatcaaatt ttagttctcc
     5401 atgaagggca tttggtagaa aaaggaacgc acgatgaact tcgttttgcc gatggtatct
     5461 atcaaaaatt atgggaaatc taggagaggg aaacaattta tgcagaataa taattggttc
     5521 gaacattcta gtatctatac taaacaacat catagcttct accgttgggt gttataccct
     5581 gtaattcttt ttttatgtat tattggattg tttttaacct ttgctaaaaa agaggttgtt
     5641 attcggacaa cggctcaatt gaaccccgaa aaaatagaga agttacaagt gccattagaa
     5701 gctaaaataa tggaaaactt cctaaaagaa aatcaatttg ttcataagga cgatgtgttg
     5761 gttagattgg attgttcttt aatagagaat gaaaaggctc agatagaaca agaaaaccaa
     5821 cgtattactc aacaaataaa gatggctcag ctatttattg aaagtataag taaaggaaaa
     5881 aatttgtttt caacggatga cagttttggc tacagtaatc aattaaagag catgttgtca
     5941 gaaaaagaat cactccgcta cgctttgaag caaagtgaat taaatgatca aaagcaatta
     6001 gaagtatacg aaaagacaaa aagacaacta gaaaaacaaa ttgagagttc agatagtaaa
     6061 ttacaagaat ggcaacaagt acaggtagct tggagtaata atcaatcatt aaaagatttt
     6121 tcaaaagaaa tgatggcaaa ctatgagaat tggcaagaac aactaaataa tgtttctgat
     6181 gatcaaaaaa atcaagtgaa actgacaatt tcagcaagca taaatgaaca aattgagcaa
     6241 ctaaaaaaag aagtagaaca gtatcagtca gaaaaagcta aattagttaa accaactact
     6301 tctgagaatg acagaattag tcaaacggaa aaaggaaagc aagagctaga acaagcaata
     6361 acaactgtaa aacaaacagt tacagagcta aatgaaaaac aagagaagaa ccagtcagtt
     6421 atcaaaagtt tggacgatca actttcaaaa ggaatactaa aagctcctgt atctggaacg
     6481 gttcatttaa atgaagaaac aaagggacaa gcagaactag ccaaaggaac agtgcttgca
     6541 gaaatttatc cgatccacga aaaatcaaaa atgaaattta cagcactttt acctgcgaat
     6601 gaaagtacct atattaaaga agggatgaag gttcatttta aattggatca aaaaggtggg
     6661 gctccgatta cgatcgacgg aactttagat gaaatttcag aaaattctac tgccactgag
     6721 aaaggtgttt tttatgcagt gaaaggaacg ttaaaaacaa ctcaatcgtt tccgtttcgt
     6781 tatggattaa ctggagagtt gtctttaatt gtaggagaaa aaacatattg gcaacaaatt
     6841 gtcgatgtgt ttctaaataa aaaataatta tttttactaa gaatatagga ggagtttata
     6901 tgaataaaag aagatggatt gctgtgggag tagcgtgcgg attattgtta ctatccatta
     6961 tcgtcgcgct aattccagga aaagataaag aggaagcatc aaatacaacc ctaacaggaa
     7021 taaataagat tttttacgga tctaacgaaa tcacagaaga aacgttagaa gaaggcgcat
     7081 caaataaaaa gattgtcaaa ctatccgtca atggagttat tgcagataca ggtgaaagca
     7141 atctttttag tagggaacaa tataaccatc agaatttttt aacacagcta aagaaaattc
     7201 aagaagacaa agcggttaag ggagtgttat tagaagtaaa ttcacctggg ggaggaatat
     7261 atgaaagtgc tgagattgct aaagaaatgg caaaaattaa aaagctagat attcctatct
     7321 atactgcatt taaaaataca gcggcaagtg gagggtacta catttctgct ggttcagata
     7381 aaatatttgc tacggaagaa acaacaactg gatcaattgg tgtcattatt tctggtttaa
     7441 attattctgg cttattagaa aaacttggcg taactgatgc gacgtataaa agtggtgcat
     7501 taaaagatat gatgtcacct caacacaaac cttcagagga agagaataaa gttatacagg
     7561 aatttgtgat gagtgcttat gatcgttttg tcaatgttgt agcaaaagga agaaatatgg
     7621 atactaatgc agtgaaagaa cttgcggatg gaagaattta tgatggtaac caagcagtag
     7681 aaaatgggtt ggtggatcaa attggttact ctgaagatgc attagattcc ctaaaaaaag
     7741 agaagaaatt aactgatgcg acggttatag aatataaaaa tgacacaact ggctttgctt
     7801 cttcttggtt aggtaataag attgctgaat ggcaagggct aaaggcaact acatcagatc
     7861 gtgtaatttc agtttttgaa aaacttggat tttctgatac tccaaaacca atgtattact
     7921 atggagggct ataaaatgaa taagaagagt ggcattgaaa ctttgcctaa ttatgtattt
     7981 gcgggatttt ggttacgatt ttttgctttt cttattgatt taatttgtat tagcatcatc
     8041 acccatgcta ctataggtct cttttacaaa atcatgggac tggaatctga caaatccatt
     8101 attacttttt atggtatagc gtcattagct atttatttaa cctattttgt acttttgact
     8161 aagctgaatt caggacaaac aattgggaaa atgatttttg ggataagagt agtgagcttt
     8221 gaggaaaaag agctaagttg gcaaactatc tttattcgtg agtgtatatg taggtttata
     8281 ttaaaaaatt ttgtatttca aattgggtat ttggtggctg ctttcacgcc caagaaacaa
     8341 catattgggg attatttttc taacacgtca gtggttgtta ttcgttcatt agaagctatt
     8401 aactatggtc aaaaaatttc taattctaaa acacttaatc tggagagaat taaatgaata
     8461 agagaaaaac agagtttatt ttgattttaa ttagtggaat aacagggatt ttatgtagtc
     8521 ttttagcatt gatgggtggt atgtttctat cttctattga ttcaactact ttaaatacta
     8581 cagaactaac cagtaaggat atagatatgt tagtcaatct aagcgggaat gttgttttat
     8641 ttggaggaat taccctagtt ttttcgataa tgattttcat aataggtttt ttagtaagaa
     8701 aaaatcaaaa aacagtggtc tatggtatta gtttactcat tttatctatt attccatttt
     8761 ttcttttaac attattgtgg gtaattcctg gagttttagg tattattgcg ggattgatgc
     8821 ttctaataag aaaatcttaa ttaggcaatg tgtagacaac gtgaaacgat taatcaatta
     8881 aaagctaaat acgttttgag aaatgataac ttgtagtcga tggaagacat gtagataaaa
     8941 aaactacgtg tcttttattt attctaattt tttcgaaaaa aagacactct ctgattgttg
     9001 tattaatgga aaaggggaaa gcagcaagta aatttagttt ttctattaaa tattcttcac
     9061 tttttaacct tttatagtac ttagatttaa tcctatatgt ttagagagtt cattggcagc
     9121 atgattttgc aaagcttctc gaattattgt tatattagaa actttactgt tcataattat
     9181 gcttcttttt taactcaaag tccaaagggc tacagtggac tttgagttaa aaataaaaag
     9241 ccctagttat tttgaaaaat aaaaaaagaa cagcgtaaat gttttcgaaa tgctttcgca
     9301 ccaacattta cctgttccgt gggtttggca cccaatgggc cgtgaggggc tcgaacccgc
     9361 gacccgctga ttaagagtca gctgctctac caactgagct aacggcccgt acaaaaagta
     9421 cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag aaaatcagaa
     9481 aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct cgctattata
     9541 taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt ttttcatttt
     9601 ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa aagacaagaa
     9661 ggcttagcct tcttgttttt tgttttcata gaatcccgct aatcaaatca cctagttaaa
     9721 gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg aaaaaaggag
     9781 cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa ctagaaacat
     9841 tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac caagtcagaa
     9901 cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca ttgcttacct
     9961 cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt aaaaaaagat
    10021 ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa gtattgacta
    10081 ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt cgtgaagcct
    10141 ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa ttagcacaag
    10201 gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt gctgtaagag
    10261 cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt ctagaaaccg
    10321 aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt agtagattca
    10381 ggtacggatg ttccagaaga attgataaaa aaatacgaga tgtatatgat cccattgaaa
    10441 attatttata aagatcaggt atatacagat aaaattgaca ttacccctga agagatctat
    10501 caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc gatcacagaa
    10561 atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac aatttccagt
    10621 ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga agaattggaa
    10681 gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc gatccaagca
    10741 gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct gaatgagtta
    10801 gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca aaaaggtgga
    10861 cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc aatcatctca
    10921 tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa aaaaagtttg
    10981 gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt caacttagct
    11041 gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact taaaagagag
    11101 ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt agtccacact
    11161 ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa aaaaaggaaa
    11221 cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa aataataaag
    11281 tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg aaacaaaagc
    11341 agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct ctttatgaaa
    11401 aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt caatttagcc
    11461 aaagaaggct atgacgttta tactgcatat gatggggaag aagccttgga aaaagttgct
    11521 gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga cggcttagaa
    11581 gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac agccaaagat
    11641 tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt gaccaagcca
    11701 ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg tgccacggct
    11761 gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt gacgatccat
    11821 ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca tcgtgaattc
    11881 gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga acatttatta
    11941 caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt aactgtacgt
    12001 cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt gacacgtcgt
    12061 ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa gaaaagtccg
    12121 cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt tattgatctc
    12181 gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga tcaatacatt
    12241 tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc cggaattgaa
    12301 tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat ttattgaaaa
    12361 cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga tcatccgagg
    12421 aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg ttgatatcaa
    12481 tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg tgaatatcaa
    12541 tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt acgtcaaaag
    12601 taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct ttactgcctc
    12661 actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt cgattacaca
    12721 gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact acagtcggaa
    12781 agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca atcagttagc
    12841 tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt tggatagcgt
    12901 gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag tcattacgat
    12961 caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg gacaatcgat
    13021 tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag aagatccaaa
    13081 cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga tcttgcgagt
    13141 agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag ctgtcttgca
    13201 tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct ctaatgtttc
    13261 ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat tgagcgaagg
    13321 tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag atgaaacaga
    13381 ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata caggtaatac
    13441 ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc ttgaccggtt
    13501 tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat ttacgcaaag
    13561 agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata atatcatgaa
    13621 taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat tagaaacgca
    13681 taataacgtg attttaagta tcaccgatca aggattgggg attcctaaaa aagatctgaa
    13741 ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac aaggtgggac
    13801 aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg ctatttgggc
    13861 agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg aaccttatga
    13921 ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact cattttgatg
    13981 gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg aaaaagttca
    14041 atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg aaaaacttcc
    14101 gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca agaaaccaac
    14161 agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt tggaaagctt
    14221 tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct gaactctggt
    14281 tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc atttgattta
    14341 gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac tagaatacaa
    14401 ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct cgtatatgaa
    14461 gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa agacacaaaa
    14521 tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac gaaacattcg
    14581 atcaaactga agaagtatag ttatatcctg tcacagcaac cctatacact ttttagaaat
    14641 gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga attgattttt
    14701 tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga ttttcgtgga
    14761 gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc ctatattagc
    14821 aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa tacgatcgat
    14881 tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg acagattggt
    14941 gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac cagtttgaat
    15001 acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac gaatgaaatc
    15061 atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt gatcgttggc
    15121 tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc tgaatggtat
    15181 gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt acctggattg
    15241 gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc ttttttaggg
    15301 ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa caatgtcatt
    15361 cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat cacttacacg
    15421 gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca gaccaatctt
    15481 agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc aagtggtgtg
    15541 tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga aaagaacgtg
    15601 atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa agacaatatt
    15661 ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga agaaccacta
    15721 gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac agcaaaaatc
    15781 gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat gcagacgcat
    15841 ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga ggaagcaatc
    15901 aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg gcgtcagcta
    15961 gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt ttggcatgta
    16021 gcgatcgaaa caagcgataa agtggtccaa attgagagcg tcaacgcttt cagcaatacg
    16081 atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga gtataatgaa
    16141 aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa accagctttt
    16201 aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga aagtgaaaaa
    16261 aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt gatggctcaa
    16321 attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca tcgagatcat
    16381 atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc caatcagcag
    16441 acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa acatctattt
    16501 gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg cgtctcccat
    16561 gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt cgtcatgttg
    16621 acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc cgatggttat
    16681 ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc gtggaattta
    16741 aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc acttgtcatg
    16801 actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag caaggaaaac
    16861 aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa agattttggc
    16921 gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga gctattttcg
    16981 atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa tcagggttgg
    17041 ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt atcgactgac
    17101 tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta atgcaatcgc
    17161 gtaatctgcc atgctgatcg tactgtcgct agcagcgttt ctttgtaaat aatcatcact
    17221 taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg gactgatata
    17281 agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca tattgaaagc
    17341 tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg tcttttctgt
    17401 atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg ttccttcaag
    17461 catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt tgggagcgtt
    17521 gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact caaataaatc
    17581 cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga ttgccgtcac
    17641 gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc cagtagctcc
    17701 aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt aagcagttgc
    17761 tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct ttcatggttc
    17821 gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa gttgaaggag
    17881 ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat ctgttgtgaa
    17941 tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa taattattgt
    18001 tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc agtggataga
    18061 caaaacagga ttttctatgt attttacaca agtataccag gctacatttg atgtgatagc
    18121 cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca tcgatcgctt
    18181 accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac taaatactca
    18241 gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa ctgggctcat
    18301 catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc aaagagagaa
    18361 gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg ttatagtcac
    18421 ttttacgctt atcggttcag cggtttttca acatttgttc agaatgactg ttgttcaagg
    18481 agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt ttggtgtgct
    18541 tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat ctgcgatggt
    18601 caatgggttg gttagtcccg tcttgcaagc gaattcatta gcaaatgcag aaatcttagc
    18661 ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc aatttctcga
    18721 tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt ttatgacggt
    18781 ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac cagccttttt
    18841 caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga tgatggctgt
    18901 tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt tatattttgg
    18961 attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga tcctctcggg
    19021 tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt caattagttt
    19081 tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa aggaaagaca
    19141 gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa tactcctgtc
    19201 gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt catgtttata
    19261 ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt cctacgtgtg
    19321 tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag aagaaaccta
    19381 ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc aaagagctga
    19441 tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata ctgcggcgga
    19501 atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa gcgggtgctt
    19561 ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg ccaaacaata
    19621 cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt ccaaccaatc
    19681 cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc gctaagggtt
    19741 cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac atgactgcag
    19801 ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg atcaagggtg
    19861 agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt aaagacatgt
    19921 aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat tgatttcaat
    19981 tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg atttaaaagc
    20041 ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc gaaggttaag
    20101 agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa accattaggt
    20161 gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg aatcgtttta
    20221 gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg tgaaggccgt
    20281 ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca agttatgttc
    20341 gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat cgtcgcagga
    20401 aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc aaaaataatc
    20461 aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca cgcgcagcta
    20521 tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga cctaaaggcc
    20581 gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat ggtgtgacga
    20641 tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa cttgtatcag
    20701 aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca actgttctga
    20761 cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat cctctaggca
    20821 ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat aatatctcaa
    20881 ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg ggttctgata
    20941 aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt gtcatcacaa
    21001 tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg cagtttgatc
    21061 gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct gttctagaaa
    21121 acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt ctgccattgt
    21181 tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat gtagatggtg
    21241 aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta gtagctgtaa
    21301 aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct attttgacag
    21361 gtggtacagt aattactgac gatctaggtt tagaattgaa agatgcaaca atcgaaaact
    21421 taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta gaaggttctg
    21481 gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt gctgaaacaa
    21541 cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca ggtggtgtag
    21601 ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa ttacgcattg
    21661 aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca ggtggtggta
    21721 cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga gacgtggcaa
    21781 caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc gctgaaaacg
    21841 ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta ggcactggtt
    21901 tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta gacccaacta
    21961 aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta ttaacgacag
    22021 aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca atggatccat
    22081 caatgatggg cggtatgatg taagaaaaag caacttgctt atctacaact gatcattagt
    22141 gaaagacaga taaaaagagg ctggaacaga agcgtttaac tccaagaaat aagaagaaat
    22201 tcacggaaat tgcttttcaa atttttgtga atttcagctt atttccgaag gagttgcttc
    22261 tgcttccgct gttta
//
LOCUS       DAXLPI010000041        22194 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXLPI010000041 DAXLPI010000000
VERSION     DAXLPI010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.041"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            367..921
                     /locus_tag="ACF3T5_001318"
     CDS             367..921
                     /locus_tag="ACF3T5_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3631940.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1094..1531)
                     /locus_tag="ACF3T5_001319"
     CDS             complement(1094..1531)
                     /locus_tag="ACF3T5_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3631941.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     VKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1579..2526
                     /locus_tag="ACF3T5_001320"
                     /pseudo
     CDS             1579..2526
                     /locus_tag="ACF3T5_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2797..3232
                     /locus_tag="ACF3T5_001321"
                     /pseudo
     CDS             2797..3232
                     /locus_tag="ACF3T5_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            3248..4081
                     /locus_tag="ACF3T5_001322"
     CDS             3248..4081
                     /locus_tag="ACF3T5_001322"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631942.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4326..5351
                     /locus_tag="ACF3T5_001323"
     CDS             4326..5351
                     /locus_tag="ACF3T5_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3631943.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5489..5614
                     /locus_tag="ACF3T5_001324"
     CDS             5489..5614
                     /locus_tag="ACF3T5_001324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631944.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5639..6754
                     /locus_tag="ACF3T5_001325"
                     /pseudo
     CDS             5639..6754
                     /locus_tag="ACF3T5_001325"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6837..7733
                     /locus_tag="ACF3T5_001326"
                     /pseudo
     CDS             6837..7733
                     /locus_tag="ACF3T5_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7749..8573
                     /locus_tag="ACF3T5_001327"
     CDS             7749..8573
                     /locus_tag="ACF3T5_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3631945.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8605..9915
                     /locus_tag="ACF3T5_001328"
     CDS             8605..9915
                     /locus_tag="ACF3T5_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFD3631946.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10063..11322
                     /locus_tag="ACF3T5_001329"
     CDS             10063..11322
                     /locus_tag="ACF3T5_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFD3631947.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11332..12204
                     /locus_tag="ACF3T5_001330"
     CDS             11332..12204
                     /locus_tag="ACF3T5_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3631948.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12216..13046
                     /locus_tag="ACF3T5_001331"
     CDS             12216..13046
                     /locus_tag="ACF3T5_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3631949.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13086..15269
                     /locus_tag="ACF3T5_001332"
     CDS             13086..15269
                     /locus_tag="ACF3T5_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFD3631950.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15330..16550
                     /locus_tag="ACF3T5_001333"
     CDS             15330..16550
                     /locus_tag="ACF3T5_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFD3631951.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16547..18007
                     /gene="gtfA"
                     /locus_tag="ACF3T5_001334"
     CDS             16547..18007
                     /gene="gtfA"
                     /locus_tag="ACF3T5_001334"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFD3631952.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18084..19190
                     /locus_tag="ACF3T5_001335"
                     /pseudo
     CDS             18084..19190
                     /locus_tag="ACF3T5_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19512..19721
                     /locus_tag="ACF3T5_001336"
     CDS             19512..19721
                     /locus_tag="ACF3T5_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFD3631953.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19714..19863
                     /locus_tag="ACF3T5_001337"
     CDS             19714..19863
                     /locus_tag="ACF3T5_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFD3631954.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            20017..20682
                     /locus_tag="ACF3T5_001338"
     CDS             20017..20682
                     /locus_tag="ACF3T5_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3631955.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20966..22051)
                     /gene="xerS"
                     /locus_tag="ACF3T5_001339"
     CDS             complement(20966..22051)
                     /gene="xerS"
                     /locus_tag="ACF3T5_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFD3631956.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7552 a         3331 c         4254 g         7057 t
ORIGIN      
        1 atgaaaagtt tggtagcacg gttattcata tgctaattta tttcacttta tagtaagggg
       61 agttcttagt atattgttca aacttaaatt tcatataatt taattatgta aattagctat
      121 tctaagaact tctacggata actgcccgtc taaaatttaa ataagagctt agaatcttca
      181 tataagtaag gttaattttt ttgtacaaaa aagtcggaat attctgagtt actgaaatga
      241 tataaaatca taaactaaat cccttgattt tattgacttt caaaagttta tatatataag
      301 ttaatgatta tggtaaaata tatacataat cattaactca gaatattttt aagaagggaa
      361 acaatagtga gctataatgt acaaccgtta aggacaaatg aagagataca agattttcag
      421 ttttggctaa gaagaacaag taatccggaa agagatagtt ttttattttt gtttggaatc
      481 aataatgggt tacgtatgtc agatattata ggattaaaag ttggagatat acgaggcaaa
      541 agtaagccta ttattgttga acataaaaca ggtaagcgaa aaccgatttt tatagataat
      601 ttgagagaag aaattttatt atatactgaa ggtaaagaag aaaatgattg gttatttccg
      661 agtcggcaac aaggtcgaca tataacaagg gatcgtgtct atcagatata tgcagatata
      721 gcagaaaaat taggacgaga tgatatagga acacatacat taagaaaaac ttttgggtat
      781 cactactaca agaaaactag agacattgct accttaatgt ttatttttaa tcatagctcg
      841 caagcaatta caaaaaggta cattggtata actgaagatg aaataggagc atcacttcgt
      901 ggatttaaac taggtgtata atttaaaaat ttttataaac agtaaagaca aagattaaaa
      961 gctattttta tctagaacga ttttatttca taaagttcgc ggaataaaag ctggggttac
     1021 tggtacttgg tataggttcg tgttatataa actaagtaat taaataacat ttttaggttg
     1081 gaaaaggatg aaattaaaaa aagagaatca atacggtctc aaaaacgcaa acgctgcgga
     1141 ataagaaaaa tactgataac tcaacgttta gcagactatc taatagggat agtctgcttt
     1201 tttcgttttt gaccatactt ttgaccttct gcaaacttcc ttgcttttcc tctgtagaac
     1261 tcatgaaatc tgcaaaacga attgccgttt cctctgtctg ctttttcgcg atatgggtgt
     1321 atatatcatc catagtggtt ttaatatctg tatgtccaag tcgaacatac acttctttga
     1381 cagacgcacc agattcaaag agcagactac aatgggtatg acgaaaacca tgaagtgtga
     1441 tatgttttaa gttattctct ttaatgatga ttttcaaatt gtgatttaca tagtctaaat
     1501 aaaggggctt attttcttct gatgaaaaca aaagttgttc accatctttt ataggtttga
     1561 ttctcatttt tagatattat gcccgcacgt attcatcaca tttctattgt gaatcgcttt
     1621 attcgaccga catttgactt ttatcataat atactcggtt taaaattact aatgaaaaca
     1681 attaatcaag acgatcatac catgtatcac ctgttttttt cagataatca ccaccgtgtg
     1741 ggaactgaac tgactttttt tgaagtacaa gaagggaaca accagttttt tggaaccaat
     1801 acgattgaac gaacgatact aaaagttcct tccgaagcat cattgcattt ttgggaaata
     1861 tattttgaaa cacaaggcgt ttgccattat ggaatcgaat cattttctgg acgacctatc
     1921 ttacgttttg aagggccaga tgcaacacga ttagctttag ttcccttacg cgaatttgaa
     1981 gatattgatg attatttccc gtatgtcaca gaccagatcc ctacagagca tgccatttta
     2041 ggaattgatg ccattcaact acgcgtacaa tattccgatg cgactgaacg agagttattg
     2101 tcggtaggct ggtagcacaa agaatacgtc agtttctttt cgggtcatca acgagtgaca
     2161 atcttagaaa atcaagacac tcagttctat caagaagttc agattattca agatcgtgag
     2221 aatccagttg ccattgaagg gattggtagc gtccatcatg ttgcgtttgg cgtagaaaat
     2281 aaatctgatt tacaacgcat cgacaaacaa ttacaagagc gcaattttat aaattcagga
     2341 ataaaagatc gtgaattttt tgtatcactt tattatcgtg acccaaatca attattgatt
     2401 gaaattgcta caggggaagg aaacctagat gcaaaagcgt atgaaaatca aagtccacgc
     2461 tttgaagaaa ttcctttatt tttaccgcag tacttaaatt tcattcgtga acaagtggag
     2521 caataattag ctaatcaaat ccatgacgaa gagtaagagg ttgactcata agtcaaaaaa
     2581 taaagaaaaa cgcatcaaat ccattctaaa gaatgttgat ttgatgcggt tcctattttt
     2641 ataaactatc attttcgact aataaaacta cttatgagtc agcctctctt tttttcttga
     2701 tttttatacc tctttctagg taaactaaac atctgccaaa aataccgtgt ctcattgaaa
     2761 cattctcaac tatttcaaaa taaaggggag taccaaatga acgagtatat tgaaattatc
     2821 cacgcaagag aaacaactta aaaatgtcag tctcaaaatt cccaaaaata aaattagtat
     2881 ttttaccagc gtatctggtt caggaaaatc atcgattgtt tttgatacta ttacccaaga
     2941 agctggtcgt caactgaatg acacctatga tagttttacc cgtttatttt tacctaaata
     3001 cagtcgtcca gaggttgatg agattgataa tctttctacg gcgattgtgg tcaatcaaaa
     3061 gaagattggt ggcagtgcgc gctcaacgtt agggacgatt actaacatca atgcgttatt
     3121 tcgtgtgttg ttttcatgtt ttgcaacacc tagtttagga tatgcaaatg cttattcatt
     3181 taatgatcct agtggaatgt gttctgtctg tcagggaatc ggtaagacga ttgcattttt
     3241 aataggattg ctttattttt cggctattga taatgctgat tttgtatttt ctgatccatt
     3301 taccgcagaa gttttgtctg gaattgagga acaggcaaag aatcatggtt attttgtatt
     3361 ggtgcataat gttaattcag cagaagatgt taaaactatt cagagaaatt ggcgttttga
     3421 ggggttcatt gttgttggtg tacgtgtaaa agaatttttg gaattaaata acgtttttga
     3481 tggtcctgtt gtttatattg atacgcatct ttcaaaagag acattaagag aacttgaaca
     3541 acaaatgaat cgtttatttg taaatacaaa tgattaccaa gctagtacat tggcagtgga
     3601 acatttgatt cattatggtc atgagaaaat agcgtttctt tcttttgact acattgtagg
     3661 tgaaccaagt gttattcaag aacgacatca agcttatatc gatacattaa agaaatatca
     3721 tttaaccttt aaatcgcatt ggatatataa caactctgaa tttgagcgaa tttatcaaga
     3781 attagctgaa tttactgcaa ttgttgttac tggagattat ttagcggcta aattagtaaa
     3841 atttttaaaa aataagaata aacgagtgat ggaacaatta tcgattattg gatttgatga
     3901 tattactttt gcagaattaa tggatcctgc gttgacaaca atacgactaa atccaggtaa
     3961 caagggggcg attgcttttc accaactgat tggactggcc aaacaagaag aaataaaaaa
     4021 tcaagtacta ctattagagg gagaattgat ttctcgagac tctgttcaca aaatcaaata
     4081 aaaaagctct gtgaaataag tatagttaac aataaaatat ccgaacgata gagagaatga
     4141 actgtgatct agcagttcct cgtcgcaaat tcactagaat tagcattatt tactctaaat
     4201 tgttcggata ttttttcttg tttttaatta tgattacagt gcctattttt aacgaaattt
     4261 gcttattttc aaaatgtatg attatatgtt agtaaacact ttcaaatatg aaaagaggag
     4321 ctattatggt aggaattagg gatgttgcaa aacgggcggg tgtgtccatt gcaacagtat
     4381 cgcgcgtgct aaatgaggat ccgacacttt cagtaaccga tgaaaccaaa cgaaagattg
     4441 cagaatcggt taatttttat cattatgtca aaaaaagtcc cgttgcaaaa aatttaacaa
     4501 atattgcatt aataacgaca gtttctgaag tagatgagtt ggaagatccc tattttcgaa
     4561 cgattcgcag agggattcaa gtcgaagcag aacaaagcaa agtcaatttg aagaaaatta
     4621 ttcgtttgtc agaaacaccg ttggagcaag acgatttgct aaaatgtcaa ggtgtgttga
     4681 ttattgggca ggtgctatct tctgtgattg aagaagtatc agcaattaat tcgaatgtcg
     4741 tagtgattga tgaccctagt attgaagctg aagtagatgc agtttatact gatttaacaa
     4801 aagcaacgaa tcagcattta gatcgattgt ataaaaaagg acaccgaaac attgcgttta
     4861 tcggaggagg acgtgttcgt ttagatagcc aaggaaataa acacgtgtca gatgatgatt
     4921 atcgaaaaat agcctatgag acgtggatga aacaaaaagg attagaaaaa tatagtcaaa
     4981 tttatttaga aggctggacc acacttgacg gaatgcaggc agcagattta cttttgaaaa
     5041 aacatgctaa agataagttg ccgactgcta tcatggtagg aaatgatcct attgctgtgg
     5101 gggtttatcg ttcacttcaa aaaaatggtc tacatattcc agaagatatt tcgattgtga
     5161 gttttgataa tattgaagtt gctgaatttt taacacctgc attaaatacg gtgaatgtga
     5221 atacagaaga aattggaaaa ctagcagttc gcatggttaa tgaacgaata aaagaagtta
     5281 gaaatgtttc gatcaaagtg attgtttcta ataatattat tgttagagaa agtgaaaaaa
     5341 atatcgatta aaaaagtaaa catccaaaaa agggtgttta cttttttgtt tactaatatt
     5401 tttagtaaaa aatctcgaca aaacattaag taaacagtta tggtgttgtt aacaaaaaga
     5461 aaacgcattc aaaacaggag gaattaagat gaaacatttc aaaaaaatag ctttaacagg
     5521 gatagctgtt ttaactggga cattactcgc agcatgtggt gggggagata gtaattctaa
     5581 agacgggcaa gtaactttaa gctatgcgag ttgagatact gaccaagctc caggactaag
     5641 aaaaatgtta gatgaatttg aaaaacaaaa tcctgatatc aaagtagaaa tggagacaac
     5701 gccttgggat caatattggg ttaaattaga agctgcaaca acgggaggta atatgccaga
     5761 tgtagtaacc atgcactcta gcgaatccta taaatatatg tcacagaatg ctttgatggg
     5821 acttgatgat gttatcaaag aaaatgatat tgacatggga aactttactg aaggaattgc
     5881 tgatttctat acgcttgaag acgaactata cgcaatccct aaagatgctt ctgtagtggg
     5941 gctatggtac aacaaagaat tatttgataa tgcaggtgtc gattatccag atgaaacatg
     6001 gacatgggaa acactcagac aagctgcaat tgatttaaca gatgaatcaa aaggcatcta
     6061 tggttttgca tcacatattg gaacagagac tggctactgg ccatttattt atcaaaatgg
     6121 gggagaagta tttgccgatg acaatacaaa atcaggtttg aataccccag aagcacaaga
     6181 agctttagct ttttatcgag atattgcact tgttgatcat gcttcaccaa caattacaga
     6241 attacaagat cctgataagt taagtcgttt tcaatcaggt agattagcaa tgatgattga
     6301 aggaaactgg tttgcaccgg gatttttgaa aaatgactat ctagttgaaa atgttgcttc
     6361 tgccgtgtta cctaagggaa aagttaatgc aacaattaca aatggacttg gttgggctgc
     6421 cagtgcgagt acaaaatacc cagaagaagt gaaaaaacta atggcatttt tagctagtga
     6481 ggacgccaat aaaatccaag cggaaacggg ggcatcaatc ccagcactaa aaggctttga
     6541 agacgagtgg gctgcttctt cagatacaat tgatttgagt gcttttggaa aaatgttgga
     6601 gtatggagtc cctcgtccat acaataaaga tggattacga gctgaacaag cagaagttca
     6661 aattattaac cgtattctgt ctggtgaagt aactgttgaa gatggaaccg ctcaaattga
     6721 aaaagaagtc aatgcgatat tgagcggaaa ttaaattagt taattataca atatacaggc
     6781 gaaaggattc tatttcgtct gtatattaat ggatgaatac caaaaagggg gatattatga
     6841 aaaatcgaat ttttggaaaa tatcgtaaag atcaagtgat atggggctat atttttattt
     6901 ttccaacggc attaggatta attttattaa atatatggcc agcaattcaa acagcaattt
     6961 taagttttca aaaaacggta gggttcggtg atactgaatg ggtcggatta agaaattacc
     7021 aaaaaatgtt tgcagacggt gaagtttttc agtctttatt aaacacatta atttatgcag
     7081 tggtttcgat acctagtatt gttgtattat ccttgttggt ggcagtttta atgaatagaa
     7141 aagttaaagg attgtcgatt tatcggacaa tttattttct accagtcgtt gctgcaccat
     7201 ccgcagtcgc aatgatttgg cgctggatgt tcaataatga ttatggactg attaataaca
     7261 tgttggctaa aataggtctc gatggaccag catggttaac ggatccatcg attgcgattt
     7321 attctattat cattgtagga gtttggagtg ctattggtta taacatggtg ttattactag
     7381 ccggcttata agaaattcct aaagattatt atgaagcagc ggaaattgat ggtgctagtc
     7441 caatccagca gtttttcaac attacgttac cattagttac accaacatta ttttttgtag
     7501 ttgtaacaac agttatcaat gctttccaag tatttgatgt tatttttatg atgattactc
     7561 ctaatagtac cgccatgttt aaatcacaat cattagccta tttattctat aaacattcat
     7621 ttattttaaa tgacaaggga tatggttcgg caatcgttat gttcctttta gtgattatat
     7681 taattattac tgcaattcaa atgaagttgc agaaaaagtg ggtaaactat taaggaggct
     7741 atcaaattat gaaaaataaa tttttttcaa atgcgccaat ccatatcatg cttattttag
     7801 gtggtattgc aatggcatta ccttttattt ggatgatttt aacttcaggg aaaacgttgg
     7861 ttgaatctac ccaaattcca cctacaattt tccctgagaa atttcagcta agtaactatc
     7921 aacaagtact tgatttatta ccattcatgg ctttttatat caatacattt ttaatggttg
     7981 ttggacgagt gattggctcg gttttatttt cagcaatggc tgcatatgct tgtgcgcgtc
     8041 taagttttcc agggaaaaat atccttttca gcttagtact tattcaaatg atgattcccg
     8101 gttccatttt tattactcca cagtatttat tagcacaaaa attgggtatt ttaaatacaa
     8161 ttccagcatt aatattacca ggggtagtta gtgcgtttgg tacgttttta ttaagacaat
     8221 ttttcttagg tttacctgat gaattggagg aagcggccta tttagaaggt gccaatattt
     8281 ggacgatctt tttcaaaatt atgttgccgc ttgctaaatc aggattggtt gcacttggaa
     8341 tattcacagc gttgtttgca tttaaagatt taatgtggcc attgattgtc aatacttcaa
     8401 ttgataagat gcctttagct ccgggattag caaacttaca aggacaatat gccacgaact
     8461 tcccacaatt aatggcaggt tcgttattag caatttggcc aatagttgtg atattcatcg
     8521 ttttccaaag aaaattcatt gaaggaattg ctacaacagg tagtaaatta taaaactatt
     8581 aattagtgat tggagagtaa gtacatgaca aaaattacat ttattggtgc aggaagtact
     8641 atttttgcaa aaaacgtatt aggcgatgcg atgttaactc caagtctaca agatgcacat
     8701 attgcattgt acgatattga tgaaaaacga ctaaaagaat caaatttaat gcttaaaaat
     8761 attaataaaa attcgaatga aaatcgtgct gatattgttt cttattatga tcgcaaagaa
     8821 gcgttaagag aggctgattt tgttatcaat gctattcaag tgggtggtta taaaccttca
     8881 acagtgattg attttgatat tcctaaaaaa tatgggttgc gacaaactat tggtgacacc
     8941 atggggattg gtgggatttt tagaacccta cggacactac cagtaatgtt agactttgct
     9001 aaagaaatgg aagaagtttg tccaaatgct tggttattga actatacaaa tccgatggcg
     9061 atgttaacca tgggtgtttt aaaagcgacg agtattaaga ctgttggttt atgtcatagc
     9121 gttcaagtat gcgtaccaga attgtttgag catttaggaa tagccgatca atatgactta
     9181 aatgactttc aatggaaaat tgcaggcatt aatcatatgg cattcttgtt ggaaattaca
     9241 aaaaatgggg aagatttcta tccaacaatt cgtcagttag ctgcagaaaa accaaatcca
     9301 catccagatt ctgtacgttt tgaattgttg aaacgttttg gttactatat tacagaatca
     9361 agtgaacaca atgcagaata ccatccatac tttattaaat caaaataccc agaattgatt
     9421 gacgaattag ggattccgtt ggatgaatac ttacgtcgtt gtgaacaaca aattaatgat
     9481 tgggaaagca tgcgagagga cattgtaaat aatggggagt taacacatac gagaagccgt
     9541 gaatatgctt catatatcat ggattctatt acgacaggaa ctccgtcagt gattgcagga
     9601 aatgtcttaa ataaaggctt aatcacaaac ttaccgacag atgtttgtgt ggaagtacct
     9661 tgtttagtcg ataaaaatgg cgtgcaacca acctatgtcg gtgaattgcc aacacaatta
     9721 gctgctttaa atcggacgaa tatcaatgtt caagaactga cagtagaagc cgctttaaca
     9781 ctagataaat caaaaattta tcaagcagcg tatttagatc cacatctatc atctgaattg
     9841 tctttaacag atatcaaaaa tttagtggat gatttaatcg aagcacatgg ggactattta
     9901 cctgaatata attaaaaaga agctgaaaga gtaaggaatt ttcaattcct tactcttttc
     9961 ttagttaaat ttagtaaaaa tattgacaaa acacttagta aacagttaca ttagtgttaa
    10021 caaaaagaaa acgcattcaa attgtataag gaggagcata aaatgaagtg gtctaaaaaa
    10081 attattttag cttgtatagc actaggagca acgatctctt tgactgcttg tggaaaaaat
    10141 caggaagatg caagcggtaa ggtagaaata gagtttttta atcaaaaaaa agaaatgact
    10201 caaacgattc aggagattgc taaagatttt gaagcaaaaa accctgatat tcatgtaaaa
    10261 gttgtggatg taccgaatgc tggtgaagta attaaaacac gtatgcttgc aggcgatgta
    10321 ccagatgtca ttaatcttta tcctcaaagt atagaattga aagaatgggc gaaagctggt
    10381 tatttagagg acttaaccga agaaccgtat ttagagaata tcaaaaatgg atatgcacaa
    10441 cgttttgcta ttgaggatcg tgtatatagc ataccattaa ccgctaatgt ctatggtttt
    10501 tactataaca aaactgcttt tgaggaaatg gggattcaag caccagaaac ttgggcagaa
    10561 tttgaaaaaa tagtagctga gattaaagat caaaacaaag taccgtttgc gattgcagga
    10621 gcagaaggct ggactttaaa tggttatcac caactagcta tagcgactgc tgctggtggt
    10681 gaagaggaag cgaacaatgt ttggcgtttc tcagaagtta atggaattaa tgctgaaagc
    10741 aaagaaatgc aaaaagattt taatcgatta gatttgttaa gagaaccaaa agctttgcaa
    10801 aacaactggc aaggtgccgg ttataacgat acagtagtta cttttacaaa aggtgatgcg
    10861 ttgattatgc cgaatggttc atgggcaatg ccgatgattc attcacaaaa tccagatttt
    10921 gaagtaggaa cttttccttt tccagcagat gaagcaggtc aaagtctaac gattggcgca
    10981 ggagacctag ccttatcagt ttctgcaact tctcaacata aagaagaagc caaacgtttt
    11041 gtagaatata tgacaacacc tgaagcgatg cagaaatatt atgatgtaga tggtgcaccg
    11101 tgtgctgttg agggcgttat cgagaatact caggattcac cattaagtgg tttaactgaa
    11161 cttgctttta ctgatcgtca tttagtttgg ttagccaaag attggaatag tgaaaatgat
    11221 ttttatacct taacaacaaa ttatttacac aatggcaatc aaaaagccat gattaacgca
    11281 ttaaatgctt tctttaatcc aatgaaagct gacggaaggt aggaaaataa aatgaaaata
    11341 aaagaaattt taaatcgtta ctgggcaatc atttttgtag caattccaat tattttgcag
    11401 atgatttttt tctactttcc aatggttcaa ggtgcttttt acagtttaac taattggact
    11461 ggtctgactt ataattttga ttttgtgggt ttgaacaatt ataagattct tttttctgat
    11521 ccgaagttta tcaaatcgat tggcttcact ttaattgtga cgctttgcat gattgctgga
    11581 caaattggtt ttggtatttt gattgcgcgg gcgttgaacg caaatattaa agggaaaaca
    11641 ttctttagag catggttctt ttttccagcg gtcttatcgg gcgtggttgt ttctttagta
    11701 tttaaacaaa tttttaacta tggtttacca aaaattggta gtatgttagg cattgaattt
    11761 ttacaaacca gtcttttagg aacaacatgg ggagcgattt ttgcagtaat ttttgtttta
    11821 ctttggcaag gaattgccat gccagtcatc attttcttag cgggttttca aagtattcca
    11881 caagaaatta ttgaagctgc tagaatggat ggggcgaatg ataagcaaat tttttggaat
    11941 attgaaatca cctacttatt accaagtatt tcaatggtat ttattatggc attaaaaggt
    12001 ggcttaacag catttgatca aatttttgct ttaactggtg gtggaccaag cgatgccaca
    12061 acatctttgg ggttacttgt ttataattat gcttttaaaa acaatcagtt tggttatgcg
    12121 aatgctattg ctttagtctt atttatctta attgggattg tttcactggc acaattaaaa
    12181 ttatcgaaaa aatttgaaat ctaggaggtt tcccaatgaa aacatcaaaa gaaaaaaata
    12241 tttctaaata cttgctttta ttattagggg gcttcatgat tttagttccc ttagtttcaa
    12301 cgctattcgg ttcatttaaa acaacacccg atattatgaa taatttcttt cgttttccta
    12361 atccagcgac tttggaaaat ttccgaagat tgttagcgga tggaattggt cattactttt
    12421 ggaattcggc aatcatcaca atcattgcag tagttgctgt gacagcaatt attcctatgg
    12481 ctgcttattc actagcaaga aaaatgactc gtgggaaagc atacggattg atgtatttat
    12541 ttttaatttt aggaattttt gttcctttcc aagtaattat gattccgatt accgttatga
    12601 tgagtaaaat tggtttagca aatatgtggg gattaatctt actctatctc acttatgccg
    12661 taccacagac actattttta tatgttggtt ttattaaact aaatgtgcct atcagtctgg
    12721 atgaggctgc tgaaattgat ggggctagtc actttacaac ttatttcaaa attgttttcc
    12781 caatgttaaa accaatgcat gcgacgacta ttattcttaa tgctctatgg ttttggaatg
    12841 atttcatgct accattatta attttaaatc gtgattctaa tatgtggaca ctaccattgt
    12901 ttcaatataa ttatacggga caatatttta acgactatgg tccaagtttt gcttcctata
    12961 cagtaggaat tgtcacgatt acactagttt atcttgcttt ccaaagaagt attatagctg
    13021 gaatgagcaa tggagcagtg aaataaccaa taaattattc tcattataaa ggagttcata
    13081 aacttatgat tatatttgac aatcacacac aaacatttca tttacaaaat aaaaaaatta
    13141 gctatctatt aggaatcgaa gaaaataatt atttaacgca tctttatttt ggaaaaaaga
    13201 ttacgcacta ttctggtggg tatcgctacc cccgaacaga tcgttccttc tcacccaaca
    13261 atgcaaatgc gcaagatcgt ttattatctt tagacacatt accattagaa tttccaggat
    13321 atggacatgg tgattttaga gaaccggcgc ataatattaa attaggaaac ggtagtcgtg
    13381 taaatgattt tcgttatgat agctacgaaa ttcaaaaagg aaagcccagc ttagcgggat
    13441 tgccagcctt atatacggaa gatgatgatg aagctgaaac gctgattatt caattgaaag
    13501 acgcgattag tggattgact ttacagttgt tctacacaat ttatcaaaat cacagtgtaa
    13561 ttattcgttc tacgcaatta gtaaataata gtgatgaaac agtggagatt aaccgcttag
    13621 ctagtcaatc aattgatttt ccaaatcgtc cactcgagtt aattcattta aatggtacct
    13681 gggcaagaga acgtcaaatg actagagaaa aaattcatac agggataaaa gtattagata
    13741 gtaaacgcgg atcaagcagc catcaacaaa atccgtttgt tgctttagta gatgccaaaa
    13801 cagatgaatt tcaaggagag gcatatggat ttaatttagt ttatagcggt aatcacgaaa
    13861 cggtgattca aaaagatcca tttgaccaaa cacgggtcat tatgggaatt aattcattta
    13921 attttggttg gcaactaaaa actggcgact catttcaaac gccagaagtc gtgatggttt
    13981 atagtaatga aggtctgaat acgttatcac agacgtatca tgaattgtat aatcaccgtt
    14041 taattcgtgg aaactatcgt ttaaaagaac gtcccacgtt aatcaacaac tgggaagcaa
    14101 cgtattttga ttttaacgaa gaaaaaatca tgaatattgt cgatgatgcg aagaacttgg
    14161 gcattgaaat gtttgtttta gatgatggtt ggtttggcgg tcgtgaaaat gacttcactt
    14221 cattaggtga ttgggttgaa acagaaggca aacttgcttg tggtttggaa aatttagcca
    14281 agcaagtgca tgataacggg atgaaatttg ggatttggtt tgaaccggaa atgatttctg
    14341 aagatagtga actatttcga gcacatccag attgggcatt gggtattcca ggtcgtgacc
    14401 gctcaattag tcgaaatcaa tatgttcttg atttttcaag acaagatgtt cgtgataata
    14461 tctatcaaca aatgacggcg atttttgatc gtgtaccgat tgattatgtg aagtgggata
    14521 tgaatcgaaa tatgacagaa gtctattctg tcttgttgga ctcaacgatg caaggtgaag
    14581 tggctcatcg ttatatgtta gggttatatg aatttttaga aaagttaaca acgaattatc
    14641 caactatttt atttgaaagt tgttcgggtg gaggcggtcg ttttgatgca ggtttcttgt
    14701 attacatgcc acaaacttgg acgagcgaca ataccgatgc cgtggctcgt ctgaaaattc
    14761 aatatggaac cagcttagtg tatccaattt caagtatggg tgcgcatgtt tcagcagtcc
    14821 caaatcatca aacaggaaga gaaacgagtt taactattcg tggcaatgct gcgatggctg
    14881 gtgtttttgg ttatgagcta gatctaacgc aattaacttc tgaagaaaaa gcagaaatta
    14941 aagaacaagt tgccttttat aaaaagcatc gccaactttt gcaatatgga actttttatc
    15001 gcttggaaag tccgtttgaa ggcaatgata ctgcatggat gtttgtctca aaagaccaaa
    15061 cagaagccat ggtcttttat ttccgagtat tagcagaagc tgcgcatcca ttagtcacat
    15121 tgaaattagc aggattagca gaagaagcaa cttatcaaat ggaagatgta acatttgctg
    15181 gcgatgaatt gatgaatctc ggtttttata ttgactcaga actacatggt gattatgcga
    15241 cacaacgctt ttatttgaaa cagatatagt agcttgaatt tacaatataa gtgcaacacc
    15301 aaaagagact cattccaaac gaagaaaata tgttttattt acacgcattt actaaaaagc
    15361 agcctgattt gaattgggaa aatgaagaag tacgtgaaga aatctatgca atgattaact
    15421 attggttaga taaaggacta ggcggattta gaatcgatgc gatattaaac atcaaaaaaa
    15481 aactagagta tggcatcttt gaatctgatg gagaagatgg gttagccttc attgggccct
    15541 ggattcttaa tcagtcgggc attgaagtgt ggttaaaaga aatgagagag cggacattta
    15601 aaccacataa tagtatgacg gtagcagaag ccgatgttcc taatgaacgt ttagatgaat
    15661 atattggtga agatggctat tattcgatgg tttttgattt tagttatacg gatattgatg
    15721 tacctgaaac tggtgaatgg tttaaagata gccaatggac atggacggat atgcgtacca
    15781 atatttttac gaatcaatta gttacgcaag ataaaggttg gggcgcctta tatttagaaa
    15841 accacgatca accacgttca attaataaat atattccaga agaatggatc aatgattata
    15901 gtaaaaaaat gttggcgacg ctttttatgt tactaagagg tacccctttt atttatcaag
    15961 gacaagaact aggaatgacc aacatagaaa tggcatcatt agaagatttt gatgatgtag
    16021 ctacgcattc tcagtataaa cgggcattag aatatggatt ggcaccagag caagcattga
    16081 aagctgtcaa taaacgaagt cgggataatt ctcgcacacc tatgcaatgg aatacgcaaa
    16141 aaaatgccag attctcaact tctgataatg tatggttaaa agtaaatcct aattatccag
    16201 aactcaatgc agaagctcag ttaaccaatc aacagtcggt attcaattat tatcatcaat
    16261 taattaaatt aagaaaaaat agtgtataca aagacgtgct aatctacgga caatttctac
    16321 cagtgaaaga taaaaatgaa catattttgc tttatgaacg ccaattagaa gataaaatcg
    16381 tattagttat cttaaacgtt acagcaacag aacaagaata ttatgtagat gaacagtacc
    16441 aacaagtatt aatcaataat tatgaagatg ttttattaga tcaaagtcga aaattgcgtt
    16501 tacgaccgtt tgaaagtatt gtattagcta attatggagg acaattatga aaattaaaaa
    16561 cgaagcaatg ctcatcacct atgcagatag tttaggaaaa aacatgggcg aattaaaaca
    16621 agtcttagac aaacatttac aaggtgttat tggtggtgta catctgttgc ctttttttcc
    16681 atcgacaggt gatcgaggct ttgcgccgag tgattatacg acagttgatc cagctcttgg
    16741 aacatgggaa gatgtggaag cattaggtga agattattat ttgatgtttg attttatgat
    16801 taatcacatt tctcgtgaat caaaattttt ccaagatttc aaaaaaaacc atgagcagtc
    16861 accatacaaa gaaatgttta tccgtattca tgaatttttc ccagaaaatc gaccaacaca
    16921 agaagatatt gatttaatct acaagcgtaa agacaaagcg ccgtttcaaa cggtagaatt
    16981 tgcggatggt acgacagaag aggtttggaa tacctttggg gaagaacaaa ttgatttaga
    17041 tgtgaccaaa gaagttgtga aagaatttat tcgagaaacc atcaaagata tggcgaacca
    17101 tggctgttct ttaattcgat tagatgcgtt tgcttatgcg attaaaaaat tagataccaa
    17161 tgatttcttc gtggaaccag atatttggga acttttagac gaagtgcgtc aagaagcagc
    17221 gaaatatgac gtcgaattat taccagaaat ccatgagcat tattccattc aaatgaaaat
    17281 tgccaatcat gattattata tctatgactt cgccttgcca atggtcactc tgtattcact
    17341 ttatagtgga aaatcagaac gtttagccaa ttggttgaaa atgagtccga tgaagcagtt
    17401 tacaacattg gatacgcatg atggaattgg tgttgtggac gctcgtgatt tattaacaga
    17461 tgaagaattg gattacacct ctgaggaatt gtataaagtc ggggcgaatg tgaagaaggt
    17521 ctattcaagt gccaattaca ataatttaga tatttatcaa attaacagca cctattatag
    17581 tgcattaggc gataatgacc gtagctatct gttagcacgt gcgattcaag tatttgcgcc
    17641 agggattcca caaatttatt acgttgggtt attagcagga aaaaatgata ttgaactgtt
    17701 ggaagcaacg aaagaagggc gtaatattaa tcgtcattac tatgatttag ccgaaattga
    17761 agaagaagtg caacggccag tgattcaaca attatttgca cttttaaaat ttagaaatga
    17821 atctgcggcg tttgatttgg atggaacgat tgaagtagca acgccatcag aatcagaaat
    17881 agtgattaca cgtaaaaata aaaacggtag tgatagtgca acattaacgg ctaacttgga
    17941 tacgaaagaa tttatcattg agtctaaaga tataaaaatt ttcactagtg ttgcattaaa
    18001 attttaacct attccataga acaaaaaatc ctttatacta gcacttggat gacttatcca
    18061 agaccaataa aaaggactca tacatggata agtataaaac aaatacagca tttaataaat
    18121 ggttttctgc cataaaatta ggaaaacttt ctgtcaactc ccaaaaatgt atttttgatt
    18181 acaattcgta tcgtaaaaaa ctgtcttttg aagctgtgct caagctcttt ctctacgcag
    18241 ttaatgaaga aaaagaaagt ttgcgtgatt tgagtacagc tcttatcgac cgatcgctcc
    18301 aactagaaac cgatgtgtca tcgattagtc atacccaatt gtctcgagca tttaatgcac
    18361 ttgaacctaa agtgttagaa gaaatttttc aacaactgtt agaaaaagtt caacatcaaa
    18421 tcaaaccaac caagcgaaat catctgtatc tgattgattc cagtacgttt tcgcttagtt
    18481 taaaacgtca taagtgggcg actttccgtc aaacaaaatc aggtgtcaag ttacacttaa
    18541 actattgtta tatggacgat actacgatgt atccaactga ttttacctta acaaatgccc
    18601 atgaacatga tctcaatcaa ctaccagtat tggtgaatca accagaggca acttacgtgt
    18661 ttgatcgtgg gtattaggac tttgaaaaga tggatcagat gcattgggat ggctatttct
    18721 tcgtcactcg gattaaaaaa aacaccaaag ttcacgtgat agatactctg gaaacatcgc
    18781 cagaaacaga aattctacga gacgagctgg ttcgtttagg atctaaaacc tatctaaccg
    18841 ccaatttccg actagtcact gttcaagata agaacggacg tgtgtttcaa tttatcacca
    18901 atcgaatgga cgtctcttca aaagaaatct ctgatatgta tcacgctcgt tggcaaatcg
    18961 aattgttttt caagcacatc aaacaacaca tgaccattaa aaccttcttt tctcaaagtg
    19021 aaaaaggcgt gcagaatcaa ttgattttaa cgatgatttc cgctctcctc acgtttctca
    19081 tcaaattgga gacgaaaacg gaaaaatcag tgttccaaat caaacgattc tttcgttatt
    19141 tactgtttca gccctttgaa tgttgtctag aaaaattaat tcccacatga gaaaaaagaa
    19201 atttggctcg ttacttttgg ttttcagttg aataacagat aaaaaatttt ctggaaaaag
    19261 tcatcctctg cgtcagcaca gttttttttg acttattttc agaggaaata gttttcagaa
    19321 tattctaaaa tgttttatgc aacactagtg atatttttta gaattagatt tctaatgtgc
    19381 attttttgag tattaaggag caagtataca aagatttata aagctgatta aatttgaata
    19441 aaaatatgca ggaacgctac tatttttagt agtgttcttt tttatatttg aataaaaaat
    19501 gtggtggtta aatggtggtt ataacaaaaa atgaattaat gaaattaggt tatgggaagt
    19561 atcagtctga gagaatcata cgacaggcaa aagcaattat ggtttcaaaa agctatacct
    19621 attacgaaaa tagaagatta ggactagtac ctgttgaatc tgtagaagaa gttttaggta
    19681 tgaagataga ggtagaggag gattttcaga atcatggtta attttaaaaa agataaaaat
    19741 gggaaatatc attttactgc tagtttggga ttcgacaaac gaacagggaa aagagtacaa
    19801 aaaaacgtag aggggttaaa acagttaaag aagaaaaaaa gcgtataccg aacttgtttc
    19861 taattatgga aaagaagcag aaacttctca aagtactatg ttgtttgagg agttttttta
    19921 taaattattc ctaccttatt atcaagggaa agtagaagaa agaacatata aaaatcggtt
    19981 atctacaatc aaaaaaactt ttcttatttc tttaaaatgg agttaagaga aatacgtcca
    20041 attgacattc aaaagtggca aaataattta ttgagcaaat ataaaaattc ttatgtaaga
    20101 aacatgtatg ggatttttca gatgtcatta gatcgagcag ttgttttggg actacttgaa
    20161 aaaaatcctg caaaagttat tggaaatgtg aagaaaagga aaactacagt tgatttttgg
    20221 acaaaagaag aatttacaaa agttatttca actttttata ttgatgattt gtaccaacat
    20281 tttggtttta tttgtatgtg gttacttttt atgaccgtta tgcgtttggg agaagccact
    20341 gcattaactt ggaaagatat tgattttgaa aataaaaact tgcgtgtgaa taaaacactc
    20401 tattataaga gagccaaaac ctatgagttt gtagaacccg aaacggcttc tagtattcgt
    20461 acaatcgaac tagacgacaa tacaattacc tttttaaaag cttggaagga gcgtcaagtt
    20521 tcaataggag gcatgtcgtt tgtactttct tataatggaa cacctactca gaaatataca
    20581 atccgtcata ttatgagaag acatgcaacg atcgttggtg ttcatataat ccgcatacac
    20641 ggattaaggc atagtcatgc ttctttgctt atatcacaat aaaaagatgc acttcgaatt
    20701 aaagaaaggt taaaacatgc tgatgtacaa acgactttag gaacttatgg tcatttgtat
    20761 ctaaattcat tgagagagat aacaaatgat cccacagatt atatccaaac taatttttct
    20821 aaaatcaaac cgacacctat ttacgataat aaaaaataaa tgataaacaa aagtaggatc
    20881 acaccaaaag aaacacacta gaaaaactat aatacaggct tttctagcgt gttgtttctt
    20941 atttccgcct atttatacat tatactcata aagcatttaa tgcatcgatc gtagccgcat
    21001 tgtctatatg ggtatataaa gttgttgtat tggtacttga atgtcccaat tgttgggcag
    21061 ttaataccaa tgaatttgtc tgttggtaga gtctagtagc taatgtatga cgcagtttgt
    21121 gagggctgac ttgaattcta aagccttttg aatattttgc taccatccgt tcgattgcaa
    21181 taccggatat acgcttggct tctccttggt aaattgctag aaataaagct ttttcgtttt
    21241 ctgttacttt atatcgttct ttccgaatag ctagataatt agctaaatat tccattgcga
    21301 atgctgctat attgacagca tctcttttat ttccttttct agttactacc acattattct
    21361 tatctaaaga taggtcatct aaattaatat ttataacctc ggagagtctc aatccagatc
    21421 ctaaaatcag tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag
    21481 ctcgtttact caaagtattt ttatattcat tggcaatata atctagataa ttttgtgtat
    21541 ccttatctag aaataatttt ttctcaatgg cattggcacg tgcagctaac gtagttttat
    21601 ctgtgatggt tgctacttga tccataggat tattgtctaa gtatggtttt cctagaggat
    21661 tattggaact tttcgaaaga tatttaaata gaacctttaa tgccgtgata tttctattga
    21721 tagtagaagt agatagacct aagttattat tatctttgag cttagttttt gttgtctctt
    21781 tttttacatt ttcttttgtg cgacgtttca aatgagattt gtacaagttt aggtcatagg
    21841 cagttaactg tcttatcgag gtgatagaag tatcaggaac gatagactca ctcagaagcc
    21901 attcataaaa gagcttataa ttttttagat attctaaagt ggttcgaggt gattgatctt
    21961 tatcctcacg atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat
    22021 ctattttttg atatatttct gtatttttca tgtcattcat cctttcaata agtaggggag
    22081 aagagtatct ctataattgg actataccaa ttatagaatg ttttgtcaat tggtatgctc
    22141 taaaatttat taatttgttt caaaaaaagc atataaacag gtatacgttt aata
//
LOCUS       DAXLPI010000042        21970 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXLPI010000042 DAXLPI010000000
VERSION     DAXLPI010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.042"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            4..816
                     /locus_tag="ACF3T5_001340"
     CDS             4..816
                     /locus_tag="ACF3T5_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFD3631957.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="ACF3T5_001341"
     CDS             813..1613
                     /locus_tag="ACF3T5_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFD3631958.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="ACF3T5_001342"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="ACF3T5_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFD3631959.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="ACF3T5_001343"
     CDS             3110..4819
                     /locus_tag="ACF3T5_001343"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFD3631960.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="ACF3T5_001344"
     CDS             5031..9389
                     /locus_tag="ACF3T5_001344"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFD3631961.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="ACF3T5_001345"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="ACF3T5_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFD3631962.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="ACF3T5_001346"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="ACF3T5_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFD3631963.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="ACF3T5_001347"
     CDS             11227..11520
                     /locus_tag="ACF3T5_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFD3631964.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="ACF3T5_001348"
     CDS             11517..11828
                     /locus_tag="ACF3T5_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFD3631965.1"
                     /translation="MNTVNRQEAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="ACF3T5_001349"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="ACF3T5_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFD3631966.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNPNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="ACF3T5_001350"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="ACF3T5_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFD3631967.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14678..15134)
                     /locus_tag="ACF3T5_001351"
                     /pseudo
     CDS             complement(14678..15134)
                     /locus_tag="ACF3T5_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            15299..17661
                     /locus_tag="ACF3T5_001352"
                     /pseudo
     CDS             15299..17661
                     /locus_tag="ACF3T5_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
     gene            18029..18952
                     /gene="truB"
                     /locus_tag="ACF3T5_001353"
     CDS             18029..18952
                     /gene="truB"
                     /locus_tag="ACF3T5_001353"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFD3631968.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            18956..19897
                     /gene="ribF"
                     /locus_tag="ACF3T5_001354"
     CDS             18956..19897
                     /gene="ribF"
                     /locus_tag="ACF3T5_001354"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFD3631969.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20249..21034
                     /locus_tag="ACF3T5_001355"
     CDS             20249..21034
                     /locus_tag="ACF3T5_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD3631970.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21047..21898
                     /locus_tag="ACF3T5_001356"
     CDS             21047..21898
                     /locus_tag="ACF3T5_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFD3631971.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
BASE COUNT         7477 a         3844 c         4840 g         5809 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag cagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtggtc acagcagccg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcagtaata ggagtttacg agctgtttcg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtattg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattta atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg ttcgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcatg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcctgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccggataaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcgt attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct acaccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gattatcgga gacggtggcg caatgggcgg
     3721 gaaagactca aaagaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgcggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtactttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaat ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tcttcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaactg
     4501 gccaaaagga attgcgccat ttgatttgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaaac ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gtgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctgcatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa attagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcctattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaagaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaacccaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggggttgcg ggattttttt gttttcatta
    14581 agacgtatta cctaagagtt ccatgacttt agtcttgcgg cgtgtcagct aaccaaaaaa
    14641 gagaaaaaga aaagtaaaaa attcgacttt cttttttcta gtagtatata aatttttgca
    14701 tatagataaa gatcaagata ccgataacgg aagctagatt gtaatctatt ttagtgacag
    14761 tcaggtatag ccaagtaact agaagatcag tggaacctgc accataaaag tttgagtttg
    14821 tcggtccgtt gacagttaaa agaaaaatca cgttgaagtt attgatattt ccgattacgc
    14881 cgcttcgatt tggtcacgat ccaaattttg gataatccct gtagaaatta gcatagtagc
    14941 tgggatacca attcacatat tgacaaagat aacagagact tttacataaa acggatcaat
    15001 caggaaagga attgctcgat ccagccccag ttttccagca atgtgttaac tggcccagcg
    15061 tcattcagca aattatgcat gatgaggagc gaaatgaact gcggaacagc cattgtgata
    15121 acgacaagtt ttttggatca atgtaaaaaa cgtcagcgaa aggaaaaatt tcgtggtttt
    15181 ttatacctgt tcatttggct agatataaaa tccaaacaaa tgaacagaag tgcttattag
    15241 gtttcaattg ggagtatctg cgctagaata agagtaataa tgaaaaggag tgttatgtat
    15301 ggattattct agtaaagaat actttgataa aatgacggct tggtggcgtg ctgctaatta
    15361 tctttctgtt gggcaacttt atcttaaaga taacccctta ctgagacgta cactgaaacc
    15421 cgaagatgtc aaaaaacatc caatcggtca ttggggaacg attcctggcc aaaattttat
    15481 ttatgtccat ttgaaccgag tcatcaataa atatgatttg aacatgtttt atattgaagg
    15541 gccaggacac ggcggacaag tcatggtatc gaatgcttat ttagatggaa gttacacaga
    15601 gatctatcca gaagtaacgg aagatgagac aggaatgcaa aaattattca aacgattttc
    15661 atttcctgga ggcattgctt ctcatgcagc accagaaaca ccaggttcta tccatgaagg
    15721 cggagagctt ggatactctc tttcacatgc agtcggagct gtattagaca atcctgaagt
    15781 aatctctgct gtcgtgattg gtgatggtga agcggaaacc ggtcctttag ctggaagctg
    15841 gttctcaaat gtctttatca atccagtgat agatggagct gttttaccga tccttcattt
    15901 aaatggtgca aaaatagcca acccaacgat cttggcacgt aaatcagatg gagaattagc
    15961 aaattacttt aacggacttg gctgggaacc tttctttatt gaaggaaatg attcagaaaa
    16021 attaaatcct gttatggctg aaaaaatgga tcaagcaatc gagaaaatta aatccatcca
    16081 aaaagaagct cgcctgaaga cagcaacaga tgtcgtgatg ccaaaatggc cagtattgat
    16141 cgtacgcaca ccaaaaggat ggaccggtcc aaaagaatgg gacggggaac caattgaagg
    16201 aaccttccga gcacaccaag taccgattcc tgtagatcaa gaacatatgg atcatgcaga
    16261 tgctttactc cgttggctga aatcctatga accagaaaaa ttatttgatg cacaaggacg
    16321 tattttagaa gaaatcagag aaatcgcgcc tactggtgac caaagaatgg caaaaaatcc
    16381 tatcaccaat ggtggaatcg atccaagacc gcttattatg ccagattgga aaaaatatac
    16441 tttgcaattt gaaaaaccag gctccgttaa agcagaagat atgaccgaac ttggtaaatt
    16501 cgttcgcgag atcattgaga aaaatccaga aaatttccgt atatttggac cagatgaaac
    16561 aaaatcaaac cgattgaatc aagttttcaa aacgacaaat cgccaatgga tggaaaaaat
    16621 cgaaccagaa aatgatgaat ggctttcgcc atctggtcgc gtcatcgaca gtcaattatc
    16681 ggaacaccaa gatgaagggt tcttagaagg gtacgttctg actggacggc atggtttttt
    16741 gcaagttacg aatcattctt gcgagtggtt gactctatgt tgactcagca cttcaaatgg
    16801 atgcggaaat cacatgacct ttcttggcga aatgattatc cttcgctaaa tctcattgca
    16861 agctcaacag tcttccagca agatcacaat ggatatagcc accaagatcc aggaatattg
    16921 acacatttgg cagaaaagaa ggcggagttc atccgtgaat atctgcctgc ggatgcaaat
    16981 acattgctcg cagtcatgga taaagcattc cgctcgtcag aaaaaatcaa tttgatcatc
    17041 tcttccaaac atccgcgtgc tcaattttac tcagcagaag aagcagccgt cttagtgaat
    17101 gaaggcttga aaatcattga ttgggcttcg acagcaaaag aggaggaacc tgaattggtc
    17161 attgctgctg caggaacgga atctaactta gaagcattag cagctgtaac tttgctgctg
    17221 gaagaattcc caaaactaaa aattcggttc atcaatgtcg tagatttatt gaaattacgt
    17281 catccaagtc aagatccgcg tgggttgagc gatgaagaat ttgatcaata ttttacaaaa
    17341 gacaaaccga tcttatttgc tttccacggt tatgaaacat tggtccgcac gattttcttt
    17401 gatcgtcata accatcattt aatgattcat ggctacaaag aaaatggaga tatcacgaca
    17461 ccatttgaca tgcgtgtagt aaatgaactt gatcgttatc atttggcaaa agacgcagct
    17521 ttaaaaatca aaggaagtca agcagaagat tttgcgaaaa aaatggatca gaaattacaa
    17581 gaacatcaaa actatattcg tgaaaatggt attgatctgc cagaagtatt ggattggaaa
    17641 tggaaaaatc ttgaccaata aaatgataaa atcgatcccg caaaatagaa ttttttacat
    17701 tgattaagaa actgaaaaaa taaataaaaa aaacggaaaa aagactggaa gaatggccag
    17761 tctttttttt cgtttttggg aagtgaaaac caaactattt acctgtaagc cacccataaa
    17821 tccaatcaaa tggtgcaaag aaaaaatctt ccattctatt ccctccttta aaaaagcata
    17881 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
    17941 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
    18001 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttacctttat ggaaagaacg
    18061 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
    18121 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
    18181 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
    18241 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
    18301 agatatgtct ttatctgaaa aaaaaataga tgaagtgatg gcttcattta ttggtgagat
    18361 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
    18421 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
    18481 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
    18541 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
    18601 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaagga
    18661 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
    18721 gctgccaata gaaaaaggag tggaacggtt caaacgagta gatattactg atgaagtatg
    18781 gcagaaagta aaaaacggta tgagacttga ttatactgta tttgcgctcc cagaaatgcc
    18841 gtcagaagaa attgctttgt tttataaagg aaaagttgtc agtatttatc agccaaaccc
    18901 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
    18961 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
    19021 agtgctggga tttttcgacg gcgtgcatat cggacaccag aaagtcataa agacaggtat
    19081 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
    19141 tattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
    19201 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
    19261 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
    19321 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
    19381 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
    19441 aggtgaaaaa atcagctcta cccgtattcg tggattacta gacagcggac aagtaaaaga
    19501 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
    19561 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cggttcattt
    19621 gcctaaagaa ggtgtttatg tgtcggaaat aaaaattggg gaaacatggt atccagccat
    19681 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagagttgta
    19741 tattttggac tttaatcaag atatctatgg tgaacatgtc gcggtacgct ggaatcaatt
    19801 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaggagga
    19861 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
    19921 tttatgtata actagcaatt acaataaaat gggtgataaa taagcccagg aaaggggctg
    19981 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
    20041 tcggaaataa gccgaaattt cttgaagctg accacttctg tcacaacctc tttctttata
    20101 gaaataaaag gacactagca attcatgaaa acgattgcta gagcgtatca tttgctttag
    20161 actatatatc ttggtgtaca ctcactttaa ggcggaaata aaagttatcc gtaataacag
    20221 gtttaggtat gaaaaaggag tgggtactat gtacaaggaa ttagaaaaca aagtcgcagt
    20281 agtaactggt ggctcaaagg ggattggaac agcgattgct caacgattcg ggaaagaaaa
    20341 aatgaatgta gttgtcaact ataactcgga taaagaagga gcagaaaaag cagtcaaagc
    20401 aatcgaagag gcaggcggaa aagcagtctc tgtacaagca gatgtcagtt ctgaagaagg
    20461 aatacagcgt ttacttgata cggctttaga aaattttggg acactggacg tttgggttaa
    20521 taatgctggg atggaaaatc agcatccaac ccatgaactt agccttgaag attgggaaag
    20581 agtgttaaaa gtcaatttga ctggcgtttt tatcggaacg aaagcggctt taaattactt
    20641 tacagagaat aataaaaaag gaaatatcat caatatgtct tctgttcatg aacagatccc
    20701 atggccaacg tttgctcatt acgctgcttc aaaaggcggg gtaaaattat ttaccgaaac
    20761 ggtagcaatg gagtatgcac cgaagggaat cagaatcaat agtataggcc ctggtgctat
    20821 ccgtacacct attaatgcgg caaaatttga tgatcctgaa caaaaagaac tcttggaaag
    20881 tatgatccca ctaggacgcg taggtgaacc aaaagaaata gcggcgacag ctgcatggtt
    20941 ggcttcagac gaatccagtt atgttacagg tattacacta tttgcagatg gtgggatgac
    21001 tttatatcct tctttccaag gtggaaaagg ataagggtga ataaaaatgg gtttattgat
    21061 tgcattgatc ccggcagttg gctgggggat ccagccactg attttgaaaa agattggtgg
    21121 cagaccaaca aatgagatat taggaactgg tattggagcg gtgcttgtcg gcttactcgt
    21181 tcaattattc gcttctccag ctggtatttc agtgactaca tttttgatta gtttactttc
    21241 tggtgcattt tgggttattg gacaaacagg acagtataca tctttcaact tgattggtgt
    21301 ttctaaaacg atgccaattt cgactgcatt acagttagtc ggaacctcgt tgattggtgt
    21361 cttcgctttt ggagaatggt ctggaagtat ggggaaaatc attggagcag cagcaatcat
    21421 tcttcttgta tttggttctg ctttaacggc aatcagtgat gatcctggaa aaaaaggcgg
    21481 tatcacaaaa ggaattagtc tattagcatc aacaagtatt ggttattggg tatacagtgc
    21541 attgcctaaa ctagttgatg caagcggact ttccattttc tttccgcaaa tgatcggtgt
    21601 ctttttaggg gcagtgattt atgtgatttt ccgtaaacca accgcatttt ctgaagacaa
    21661 gagttggaaa gcaacgatcg ttggagccat ctttagtgtg tcagcattgg cttatatttt
    21721 ttcagcaaat gaaaatgggg ttgctactgc atatatcatt acacagctaa acgtagttat
    21781 ctctacatta ggcggtatgt acatcctgca tgaaaataag acaccaaaag aaaaaaaatt
    21841 aacgattatc ggattattgc tgatcgtagt aggaagcatg gtcacggtct ttatttgagg
    21901 ataaagatgg taaaatttca tttattaaaa aaatatttgt ttgaaccaaa cagtaagtac
    21961 ttataaaaaa
//
LOCUS       DAXLPI010000043        21928 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXLPI010000043 DAXLPI010000000
VERSION     DAXLPI010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.043"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..142)
                     /locus_tag="ACF3T5_001357"
     CDS             complement(<1..142)
                     /locus_tag="ACF3T5_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3631972.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..497)
                     /locus_tag="ACF3T5_001358"
     CDS             complement(183..497)
                     /locus_tag="ACF3T5_001358"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFD3631973.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQ
                     RYGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQ
                     KN"
     gene            complement(548..802)
                     /locus_tag="ACF3T5_001359"
     CDS             complement(548..802)
                     /locus_tag="ACF3T5_001359"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3631974.1"
                     /translation="MTRYEENFKQMIIELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(1067..1630)
                     /locus_tag="ACF3T5_001360"
     CDS             complement(1067..1630)
                     /locus_tag="ACF3T5_001360"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFD3631975.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLIDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(1645..2688)
                     /locus_tag="ACF3T5_001361"
     CDS             complement(1645..2688)
                     /locus_tag="ACF3T5_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3631976.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(2821..3225)
                     /locus_tag="ACF3T5_001362"
     CDS             complement(2821..3225)
                     /locus_tag="ACF3T5_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFD3631977.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(3425..3673)
                     /locus_tag="ACF3T5_001363"
     CDS             complement(3425..3673)
                     /locus_tag="ACF3T5_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFD3631978.1"
                     /translation="MHWLWVLIVGAIIGLIAGAITNKGGSMGWIANIVAGLVGSAIGQ
                     ALLGSWGPQLAGMALIPSIIGAIIVVAVVSFFLGRSRG"
     gene            complement(4308..5255)
                     /locus_tag="ACF3T5_001364"
     CDS             complement(4308..5255)
                     /locus_tag="ACF3T5_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFD3631979.1"
                     /translation="MYSYYKIEKNQIRESSEEDYNWLVVKHGNEEEKKELLTLFDLPE
                     DIFIGSDEPEEVSRLEHLRDTKLNNPFLYTLIDLSSNHQERIEKRLEPVTFILSKGLM
                     ITYVSRESNLVERLIEKHGSELRHFEDIIAFATLMIYTHYINGLKEIKKQIDQLDAAA
                     RKTTENNELFNLADTERDMVYLDHTLKDQNLTVNHLMERKEFLDKLDNESLVYDIKLR
                     QKFANKLVTIYRDLLETIGGLFSDMMDNNLNHLMKYLDSAALVVAVPSLIAGLWRINT
                     GGLPGKDNTIGTILVFGLAVLVGILTAVYLSKKDFSKTK"
     gene            5447..6274
                     /locus_tag="ACF3T5_001365"
     CDS             5447..6274
                     /locus_tag="ACF3T5_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFD3631980.1"
                     /translation="MFLHQKELQFEAKPDKPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMKKDPAIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDLLSVLLARDSYHQNLWAQAVKELEEKEGTILVPSTFPRNEERLDIAY
                     DFYNFSEGDQSKEGSWGKGKALDGEGEYQWLSTPKVEGKAPKLDPVTPKMYGTPPEK"
     gene            6416..7255
                     /locus_tag="ACF3T5_001366"
     CDS             6416..7255
                     /locus_tag="ACF3T5_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFD3631981.1"
                     /translation="MKAPLLTLNDGNMRPAVGIGTYQIRGGEGLSQLQAAIADGYRLI
                     DSSTNYDNEGIVGKAIRRSGVPRSEFYITSKLPGKYHGYDDALTSIQESLARMGLDYF
                     DAFLIHWPLPKRGKYIEAWQALVTAQKLGLIRSIGVSNFEPEHLDAIIEATGVTPAIN
                     QIEIHPYWVQEQMVAENRKRGIITEAWSPLGRGSAALKESIITDLANKYQKSAGQVIL
                     RWHVDRGILPLPKSRNLSHQRENLAIFDFELTEEEINAINRLNKEDGRIEGQDPNEYE
                     EFE"
     gene            complement(7507..8244)
                     /locus_tag="ACF3T5_001367"
     CDS             complement(7507..8244)
                     /locus_tag="ACF3T5_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008379144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HFD3631982.1"
                     /translation="MYQYVDKIHALLETVEKKEQHAIDEAIRLLTTANLEKHSIYIFG
                     ASHAGILAEEMYYRAGGMMTINAIFGRELMLDRSPITFTSKMERLEGYGTALAEIQPF
                     KEGDVLILHSVSGRNPVIIDLALAAKEKGVKIIALTNKTYSQSVTSRHSSGLRLFEVA
                     DLVIDNHGEIGDAGCQIKGMDQKVGPTSTVIGATILNTIIVEVCQQLKQAGIDYPPIF
                     YSANMDGGDELNQSLFEQYKEAIHYQL"
     gene            complement(8246..9496)
                     /locus_tag="ACF3T5_001368"
     CDS             complement(8246..9496)
                     /locus_tag="ACF3T5_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFD3631983.1"
                     /translation="MSFVTDHILKNPPVLLGLIAMIGLIVQRKPFAEVVKGSLTAAFG
                     MVALTAGVNMLTGTIAPINTAVQTQLGVQVADGLSDVTFTAEYGGTVGLAMFFGLILH
                     LLIARFTPVKTIFLTGHMLWWFPFVIVAGGVEGGMSGIPLLILGAVLSACYWSFMPWI
                     MRKYVWDATGDDSFLIGHPTGILSMVSGFVAKRVGNKEKSTEDLKVPENLSFFREISI
                     TGALVMFLMNIVVGIIAPVLVPEGGNLVMFAVQAGLNFGAGLLIMLYGVRLLINQIIP
                     AFQGIAEKVVPGAKPAFDVPILFNYRPNAVIIGFIVAMITSTILVIIVNTTNVFGILI
                     VPLVITSFFECGGAAVIGEGQGGLRGAVIGTIAASFVMVLLVGVSAVLFSTTIQNWIL
                     IFGGNDISLWGIIGRGLGSLFGGI"
     gene            complement(9499..9780)
                     /locus_tag="ACF3T5_001369"
     CDS             complement(9499..9780)
                     /locus_tag="ACF3T5_001369"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3631984.1"
                     /translation="MKILAVCGFGVGSSMVLKMTIDKVIKELGLKATAENTDLSTAKA
                     TPADVYFTSHELLNDLQASVKAPVYPIKKYMDVAEVKMQMEKFLAEREG"
     gene            complement(9793..10224)
                     /locus_tag="ACF3T5_001370"
     CDS             complement(9793..10224)
                     /locus_tag="ACF3T5_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3631985.1"
                     /translation="MINLKKLLPEDHIETTNQILTWQEAVQLASQPLLNEKAIDQQYV
                     ANMIHSVEENGPYMVLADYFALMHARPGEGVFHQGMSLLVTKNEIDLAGKSVRIFLVL
                     AAKDNQSHLESLQEIMEVFMDQEKYQLILNGDKEQICQLFQ"
     gene            complement(10221..11858)
                     /locus_tag="ACF3T5_001371"
     CDS             complement(10221..11858)
                     /locus_tag="ACF3T5_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003125786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFD3631986.1"
                     /translation="MKNNYSLIEDRRMQIFKRLINGEHLSYQQLSDEYYVSRSSIAKD
                     IAYLKTLFVKENLLLRFDNSGTYFQGSESQIQRMLKRFILLTVEHGENLSVLTNVSLF
                     EKINAAFRHSITEKQMEIPESYIQSIVVSILLIVERSNQANQLDIEEKRQFGKLFLEF
                     DKYPLIYELLKEIEEQEIYHFSSKEVQYLTYLIVGSGLRFFMKNESIPFSFRGKVRNL
                     IQKVSEGIQIDLTQDSRLEEDLTIHLYQLFLRIEAQTTVVNPLLDEIKQHYPALYGVV
                     WFSLDDFLKAYHIGISEDEIGFVSIHFQAAVERMRQLNKIVFVCPNGVGTSSFISAKI
                     RRILPNVDSIETASVAKLKEMDISAIDFIISTIDLPDVSKPVVRISPMVTNRDMKRIM
                     NHYIDLIIDNEPIADKVKILQDTRMLIAPKIYFERFETKEQALDFLISQNDFSSENQR
                     TDFQRSVLEREALQSTYLDNGFAIPHGNPEFVSKTTFSVLILDRPVDWGNQKVDIIVL
                     LMIREDEAQEVEPVMKLIMQGIEDKEWFISKMLEVKS"
     gene            12072..12965
                     /locus_tag="ACF3T5_001372"
     CDS             12072..12965
                     /locus_tag="ACF3T5_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFD3631987.1"
                     /translation="MKWRGGRQSSNVEDRSGRSAGSGGKIMVGGGIGTIVIAVLFFLF
                     SGGDLNSVTDILGGGQAANYQTENVASDEYTEEKEFSAVVFGHLEDYWNGVFEAEQTT
                     YQDPKLVLFTGSVQSACGNATSQVGPFYCPADQNVYLDLSFATELSQKYGASGDFAMA
                     YVIAHEVGHHVQNELGITQQLDKIRQQVSEKEYNQYSVRLELQADYLAGTFAKYLQGK
                     TYNGQPILEAGDIEEAITAANAIGDDTLQKEYQGYVVPDSFTHGTSKQRVEWFNRGFK
                     YGDLEHGDTFNEKNLDLVYGS"
     gene            13307..13388
                     /locus_tag="ACF3T5_001373"
     tRNA            13307..13388
                     /locus_tag="ACF3T5_001373"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13340..13342,aa:Leu,seq:cag)
     gene            13543..14046
                     /locus_tag="ACF3T5_001374"
     CDS             13543..14046
                     /locus_tag="ACF3T5_001374"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3631988.1"
                     /translation="METERQIRELEKAMNTTDNRRLYERFLAVKLVLEGHTRKEAGHT
                     IGRDEHTVSHYVKNYCHNGIQGLVSKKQSGRPSRLTDEQEAELVQIVAYHTPEEVGFK
                     SRANWTLAIVCDLILREWSYGYTQKGVADLHNFPPRYYEYPGGVIACVTAYANLQHRS
                     KWLSSKK"
     gene            complement(14259..15014)
                     /locus_tag="ACF3T5_001375"
     CDS             complement(14259..15014)
                     /locus_tag="ACF3T5_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFD3631989.1"
                     /translation="MDFEQNVRLNFDLLSDNEKDMVFFIRTHKAQVVHMSIVELGEQL
                     LSSKSSVLRLAKKLGFRGYSEMKYSLEQSIVQSVIPPTDLIALLKDEINRTFQLADQT
                     NFQPILEQLKAAKTVVVYATGFTQNNFSKDFVNDLILSGRPAMLISGETNFEMLSHTL
                     TKDDFVIVTSLSGETPSIKSTIKNLNMNRIPLCGVTELGKNFLSEHSDFLLYYETREL
                     PSNLIEGSRSMIGLNILLSILSRKYREFILFDE"
     gene            complement(15121..16791)
                     /locus_tag="ACF3T5_001376"
     CDS             complement(15121..16791)
                     /locus_tag="ACF3T5_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFD3631990.1"
                     /translation="MNEWWKSSVVYQVYPQSFKDSNNDGIGDLMGIVEKLPYLAKLGV
                     DVIWLTPIFDSPLIDNGYDIRDYEKISENYGTLEDFKRLLEQAHQLNIRVILDLVVNH
                     TSNQHAWFIESQKNKENPYSDYYIWKDPKEDGSAPTNWGSTFGGSAWEYVESRKQYYL
                     HCFAKEQPDLNWENPKVRQSIYKMMKKWFDLGIDGFRMDVISLISKHPDYPDAPNSMP
                     YTKSYYVGASNGPRVHEFLQEMNREVLSKYDSLTVGETPNTNSRQAQLYTFPERKELN
                     MVFHFDHMHLDYGKYGKFSDVRFKLSDLRATLSEWQEALSDGWNSLYWSNHDQPRAVT
                     RFGDENEYRIESSKMLGTLLHMMKGTPFIFQGEEIGMKNVPFSSIDEYQDIETNYFYK
                     KMKEEGESDEYIKRAIYLKSRDNARTPMPWDHSKGYGFSNSDPWISFSPDNQQINVED
                     ALKNPNSVFYHYQKLISLRKNYPIIQHGTYKLINENDSAVFGYIREYKNELLVVICSF
                     SKESVPYELPKSLQGRKGVLLLSNYEQSPSELEDKFILRPYEALVYHI"
     gene            complement(16795..17214)
                     /locus_tag="ACF3T5_001377"
     CDS             complement(16795..17214)
                     /locus_tag="ACF3T5_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3631991.1"
                     /translation="MKKIIFASHHNLAVGLKDTVQYILPNVGEITAIAGYIDNSPIEE
                     KINEVMVRDNEDTDILIFTDMLGGSVNQAFIKYINQPNVYLITGMNLPVVLTILLNTL
                     NKRLTDNHIREAIQEAQDQLVYVNDYLKNIVVDEEDE"
     gene            complement(17227..18036)
                     /locus_tag="ACF3T5_001378"
     CDS             complement(17227..18036)
                     /locus_tag="ACF3T5_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFD3631992.1"
                     /translation="MNNEGNKLSKKDIRRAAFRYMFMACNIFNYENQQGPAVVYGLEK
                     ALRKIYPNDEDYRASLNNHFKYFNTTTWMANLLLGASLAMEEKDGVSSLDAVQSFKTG
                     MMGPLAGVGDTLVWVLYPTIMGSIAAYMGLEGNPTGAIIWLCLNLFFLFFRFKLFSIG
                     YTSGLKLVTALGSKLSIFTESASIMGLTVVGALIPSVVKMNVAVVFQSGKVKLPIQTE
                     ILDKIMPALLPAVLTIIVYKLIASKKMTVIQIIFSVIIFALILSFFGILKV"
     gene            complement(18029..18829)
                     /locus_tag="ACF3T5_001379"
     CDS             complement(18029..18829)
                     /locus_tag="ACF3T5_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFD3631993.1"
                     /translation="MEIIQGILIIILAVWVVLDQQGLVITTWFPIMIALFAGLIMGDM
                     TTAMVIGGTFQLMALGVANIGGSSVPNWGLAALVGIYVAIRTTNNIEEAKAVALAVGV
                     PVGMLGIQLDVLAKILNTYVTHAAQKAANKMDFKKMNQILWLGPVIFGLSTAIPTALC
                     VVFGDRIVRLILDFVPKWFTDGLSIAGSMLPVVGIALLLQVMPVKKYMTMLLIGFVLS
                     AYLNMPILGISIVGFALAYYFFTTEIKKAETASPVAANIVDEGDDFDE"
     gene            complement(18844..19320)
                     /locus_tag="ACF3T5_001380"
     CDS             complement(18844..19320)
                     /locus_tag="ACF3T5_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFD3631994.1"
                     /translation="MGLVLARIDQRLIHGIVVTQWAGATKAKRLMVVDDQVSKDDTQK
                     AAMRMSKPAGTGMSIIDTDTAIINFKLGKYDSHNVLMIVKEPSTLVKLAENGIDIPKV
                     NIGIMFDGEGKQTVKKMVSVDEQEIADLKKLEAMGIPVTFHFVPSDPEEPLDVRIK"
     gene            complement(19715..20194)
                     /gene="rlmH"
                     /locus_tag="ACF3T5_001381"
     CDS             complement(19715..20194)
                     /gene="rlmH"
                     /locus_tag="ACF3T5_001381"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFD3631995.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAHISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            20623..21909
                     /locus_tag="ACF3T5_001382"
     CDS             20623..21909
                     /locus_tag="ACF3T5_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFD3631996.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTTSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSGF
                     GGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKDG
                     TKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANS
                     VTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKI
                     ASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQE
                     QILKIPSSVTNGVIVTNVQTATPAEKAGLKKYDVITKIDDKEVSSGVELQSALYKKKV
                     GDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT         6653 a         4561 c         3875 g         6839 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaataatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaacttcata aagtaaatct
      901 tttatacatt tccacttcta gatggtcatt catgttagaa gaatcaaact atttttttac
      961 tgtcgtaata atattaaaac actagaacaa atcaattaaa ttccataaaa tataaataat
     1021 agacctagca tcatctggta taaaatggat gctaggtccg ttatatttac tcaaaattga
     1081 ttctacgaat agatcttctt atcatttcta ttttagtagt catgcgttgt tgttcttcta
     1141 aaggtggtaa tggtataagt agtttagata aatttgtacc atttacattt ggttgcccag
     1201 ttccgtaaga aattttttct aataacttcc aatataatgg cgagtctaag aagcagtcta
     1261 catattctga taataatttc tcaactaacc taattcgaat caagtaagaa gcaaaaacag
     1321 attcctcact tatttcctta accaagaaac tttttcccat agttcctcct gtccttgcta
     1381 tcaaaatgtc attttcttct aatcttaaat caattagttt agaattacta atatcacagt
     1441 aaggcacact ggaccaattt acacgtccct cttgtatatc tgtgattctc aaaaacttaa
     1501 cattgccttg ttttttcgcc gaatctgtat agccatattg aatcttagta gaaatacttc
     1561 ctaaataaac ccatagtcga acatcgctag atgatttttc ttgtcgttct ttatgaacaa
     1621 cagtgatcat cttgagttgt ccgactattt tttagttgca gcaatctttt tgggacgctt
     1681 atttgcatga agcaacgttg ctaagtctag taataaaaca ttggctaaca caggattggc
     1741 tttctcgcga tacgctgcgg cgccactttc aaacaatact gctagacgat cttctacaac
     1801 aacgatttgt tccaaatacg gtggaaattt gattcgtttt aggatccggc taggagagtc
     1861 ccgctcggac actaagagtt ccgaggtata aggaccataa gaacgactgg ctaagagata
     1921 atggccataa aaagccacac cttgaatctt atcagatgtt tttctgctca ttgtcacggt
     1981 tggttggatt ttgtctttgc ggcgaattgc tgttttcatt tcagctatcg tcggcaatag
     2041 atagaaagtc aactgtcctt caccttttag tgtgaagttg ccagatacca gttgatcatt
     2101 gttttgagtc aaatacgatg cttgtggaat ctctgagaga tcagttgtca gccaataagc
     2161 gattggctgt cccgtctctt cagatgtatc tgctaagata tcgatcaaac gtaaagcggc
     2221 tactcgtcca gtagcagatc ctgtgattgt caaccataaa agttcccgtt ttgtatcata
     2281 tactaagccc cctacatgtg gccgtttagg taaaatcagc agtttgatcg gctgtcctgt
     2341 ttctctatcc agtacatgaa taatagaatg atgctgatgg tcatagcagt aggcagaaat
     2401 aaacacaaaa tcttgagaaa aagccaaccc ttgcggcgtc atccattgac aaacctcatt
     2461 ttcgccggtt tccggcacca ccattcttgt ttctgttaat ccaggaatgt tatattgttt
     2521 tgcttcagcc ttttcccaag ctgcttgaac aataggatac cgttttaact ttttttcaat
     2581 atcctgatgt atccgttgct gcttccaatg cttaaacttt aggacatagt aaacaaggag
     2641 gccaatgacc agcaacacta aaatcattat tgctacccgc tgtgtcattt ttttgcctcc
     2701 ttgttctatt cgatcaatcg tacatatacc gctattaatt accgtaacta catgtttctt
     2761 ttactagcgt ttctttatag gatctttcat aatctcattt atagtaacat taagaatccc
     2821 ttattctact atagtcactt ccacatcaat attgcctctt gttgctttag agtatgggca
     2881 aaatgcgtgg gcttcttctg cgattttctc tgcttggtcc ttctcaagtc ctggcaaaga
     2941 aacttctaaa acaaccccga tttggtagcc cccatcttct aattgaaata aagatacccg
     3001 tgctttgact tcagaatcag actcaagatt attttcttcc agcatgtgtt gaaccgcgcc
     3061 gttgaagcaa gatgcgtatc cagccgcaaa aagttgttcc ggatttgtcc cttcactatg
     3121 gattcctggg cggtcgatct tcatgtgcat acttccgttt ggtgcctcaa cctcgccatc
     3181 acggcctcca cgattgatca ttgctgtttc gtacattttt ttcattgaga tcatcctttc
     3241 tttcgtcgtt ctatattaag tgtaacgagt attttttaat ctgacaaagg taatgcttgc
     3301 attcggcgac atcagttaga caacatcgat gatcagtgat agaagcagtt tatctataca
     3361 aaaaaagaag cagcgaaaag ctgctcctta agataatagg taaaattgct gatatatttg
     3421 acgattaacc tcttgaacgt cccaagaaaa atgatactac agcaacaacg atgatcgcac
     3481 caatgataga aggaatcaaa gccattcctg ctaattgagg accccagcta cctaacaatg
     3541 cttgtccaat tgctgaacca actaaacctg caacgatgtt agcgatccat cccattgatc
     3601 cacctttatt tgtaattgct ccggcaatca aaccgataat agctcctacg attaataccc
     3661 ataaccaatg cataattttc tctttctttc tgtgaaatat ttattacaca tattatgtta
     3721 cgcttcttct taaaaataca caaaaatttt gctcttaaaa aaacaaaaaa aatttaaaac
     3781 gttgttatat taaggataag tcaacttttt tggaatcgtt aacactcact gcaagaggtc
     3841 aaaaaaactg ccgcagctct ttgaaagcgt ttcgatcagc aaaaagttat tttctgataa
     3901 agggtaccta gactatgtac gattttcttt cattgtctgc tttcaaccgt catcttccct
     3961 atgaataaaa gcaatcaaag aaacctgatt gtcgtacaca cctcattcac tacatccttc
     4021 tgatgaaaca tcaaaacaat tggcattcct ttgattctgc cttttgtatt tgaaacctgc
     4081 ttcccctcac tatgtaaaag gattatgaga aaaagcaacg atagaagtga gcgtatgcaa
     4141 tagcgagtaa agtaaaatat aaattgttcg gattgtaccc cggcctttct tcttaatatt
     4201 tttcatttct cgcttctccc ctctgcttat tggacagtct tcgcgattgt gaagtcacga
     4261 aaaaaagcaa ctttgcccag cgttcaatgc tagacaaagt tgctttttta ttttgttttt
     4321 gaaaagtctt tctttgaaag atagacagca gtaaggatgc ctactaagac agctaaccca
     4381 aaaactagaa tcgttcctat cgtgttatcc ttcccaggca gtccgccagt gttgatcctc
     4441 caaagtccag caatcaatga tggcactgca accactaatg ctgctgaatc taaatatttc
     4501 atcaaatggt tcaaattatt gtccatcata tccgagaata gaccaccgat ggtttccaat
     4561 aaatcgcgat agatcgtgac aagcttgttg gcaaatttct gtcgcagttt aatgtcgtaa
     4621 actaacgatt cattatctaa tttgtctaaa aactcttttc gttccatcaa gtgattcact
     4681 gtaagattct gatctttcaa cgtgtgatcc aaataaacca tatctcgttc tgtgtctgcc
     4741 agattaaata gttcattatt ttcggttgtt ttacgggcag ccgcatccaa ttggtcgatt
     4801 tgctttttaa tctctttcaa cccattgata tagtgggtat agatcattaa tgtggcaaaa
     4861 gcaatgatat cctcaaaatg tcgaagctca cttccatgtt tctcgatcaa tcgctcaacg
     4921 agattcgatt ctctagagac ataggtgatc atcaatcctt tggaaagaat aaatgtgaca
     4981 ggttcaaggc gtttttcaat gcgttcttga tgatttgaag aaagatcgat caatgtataa
     5041 agaaacggat tgtttaattt agtgtctcgt aaatgttcta atcgagaaac ttcctctggt
     5101 tcatcggagc caataaaaat atcttccggt aaatcaaaaa gtgtcagcag ctcttttttt
     5161 tcttcttcat ttccatgttt gactaccaac caattatagt cttcttcgga tgattctctg
     5221 atttggttct tttcgatttt atagtacgaa tacattttgc ctctctcctt taactccttc
     5281 acttctcttc aactatctaa tggcgactgc tttttgtcaa gaaaaagcgc tatttcaacg
     5341 gtttttatta atacggaaat aaagcaagcg tttgatcaga ccattctaaa taaacgtgag
     5401 agaatatttt tgggcccaat aaaaatcatt ttagaggagg acaactatgt ttctacatca
     5461 gaaagaacta cagttcgaag caaaaccaga caaaccaaat ccattaattg cgaagtatct
     5521 tcaagaattg atcggtggcc agtatggcga aatgagtgta gcaatgcagt atctcttcca
     5581 aggttggagc tgccgtggag aagaaaagta caaagatatg ttgatggaca ttgcgacaga
     5641 agaaattgga cacgttgaaa tgcttgctac aatggttgct cgtttattag aaggtgcacc
     5701 ggtagaagat caagaagccg caatgaaaaa agacccagca attggcgcaa ttatctctgg
     5761 aatgaatcca caacatgcga tcgttgctgg cttaggacca cgtccagctg acagtgtcgg
     5821 taatccatgg agtggcgctt atatcattgc cagcggtaat ttactggcag acttccgtgc
     5881 caatttaaat gcagaatcac aaggacgatt acaagtcgct cgaatctatg aaatgattga
     5941 tgaccgtgga gttaaagacc tcttatccgt gctattagct cgggacagct atcaccaaaa
     6001 cctttgggca caagctgtca aagaacttga agaaaaagag ggtacaatct tagtaccgag
     6061 cactttccct cgtaatgaag aacggttaga catcgcctat gacttttaca atttctctga
     6121 aggagatcaa agtaaagaag gctcttgggg aaaaggaaaa gcacttgatg gtgaaggtga
     6181 ataccaatgg ctttctacac ctaaagttga aggcaaagcg ccaaaactag atcctgtcac
     6241 accaaaaatg tatggcacgc cacctgaaaa ataaattgat cgaaacgaat aaaaagagca
     6301 atgattccat tcacaagaaa tcattgctct tttcttgtct tattgagcaa gttggttacc
     6361 ttaaagctaa aaaagcgtta gagtagaatg tataagaaaa aggaggaatc attatatgaa
     6421 agcaccttta cttacactaa atgacggcaa tatgcgccca gccgtgggca tcggtaccta
     6481 tcaaattcgc ggtggcgaag gcttaagcca gttgcaagca gcgattgcag atggctatcg
     6541 tttgattgat tcgtcaacga actatgacaa tgaaggaatc gtcggtaaag cgattcgccg
     6601 atccggtgtc cctcgttctg agttttatat cacctcaaaa ttaccaggaa aatatcatgg
     6661 atatgatgat gcactgacca gtattcaaga atcgctggcg cgcatgggat tagattactt
     6721 tgacgccttt ttgatccact ggccactgcc taaacgcggc aagtacattg aagcgtggca
     6781 ggcgttagtg actgcgcaaa aattaggttt gattcgttct attggtgtat ctaactttga
     6841 acctgaacat ttagatgcca ttattgaggc aactggtgtc acaccagcca tcaatcaaat
     6901 cgagatccat ccttactggg tccaagaaca aatggtagca gaaaacagaa aacgtgggat
     6961 cattactgaa gcatggagtc cattaggtcg cggcagcgcg gcgctcaaag aatccatcat
     7021 cactgatcta gcaaataaat atcaaaaatc tgcgggtcaa gtcatcttac gctggcacgt
     7081 cgatcgcggg attctaccac taccaaaatc ccgaaacctg agccatcagc gagaaaactt
     7141 agctattttt gactttgaat tgactgaaga agaaatcaat gcaatcaatc gcttgaacaa
     7201 agaagatgga cgtattgaag gtcaagatcc gaatgaatat gaagaatttg agtaaagtga
     7261 gacagcataa agctcctgag ggatttcata ggctcacatc aattatagga attggaatgg
     7321 tcacaaaaaa agaactgcga gatgacttgc cccaattgaa ttccttagat ctactagcag
     7381 caaggctcta ctgaagtctt gctgctattt tatgagaagt aaaaacttct gacacgatca
     7441 gttttcattt ggattatgct tttctcacag ttctttttct gagttaaaac ttctgtacaa
     7501 tcagcgttac aactgataat gaatggcttc tttgtattgt tcaaacaatg attggttcaa
     7561 ttcatcgccg ccatccatat ttgcggaata aaagatgggc ggataatcga ttcctgcttg
     7621 tttcaattgt tggcaaactt cgacgataat ggtattaagg atcgtagcgc caatcaccgt
     7681 tgaagtaggc cccacttttt gatccatccc cttaatctga caacctgcat caccaatttc
     7741 tccatgatta tcgattacaa gatcagcgac ttcaaacaga cgaaggcccg aagaatggcg
     7801 gctagtgact gattgcgaat acgttttgtt tgttaaagcg ataattttga cgcctttttc
     7861 ttttgccgct agtgctaaat cgattatgac aggattacga cctgaaactg agtgtaaaat
     7921 cagcacgtct ccttctttga acggctggat ttccgctaat gcggtgccgt agccttctag
     7981 tcgttccatt ttgctagtaa aagtgatggg actgcgatcc aacatcagtt ctcgtccaaa
     8041 gatcgcattg atcgtcatca tccctcccgc gcgatagtac atttcttctg ccaagatccc
     8101 cgcatgacta gccccaaaaa tataaatcga atgcttttct agatttgctg tagtcaagag
     8161 acgaattgcc tcatcgattg catgctgttc ctttttttca acggtttcta gtaatgcatg
     8221 aattttatca acatattgat acatattaaa ttcccccaaa tagactgccg agaccacgac
     8281 caatgattcc ccaaagtgat atgtcattgc caccaaagat cagaatccag ttttgaatcg
     8341 ttgtgctgaa tagaacggca gatacaccaa ctaacaagac catcacaaat gatgcggcaa
     8401 tggtaccaat gactgcgcca cgcaagccgc cttgtccttc tccaataact gctgcgccgc
     8461 cacattcaaa gaaactggtg ataactaatg gtacgatcaa gattccaaaa acattcgtcg
     8521 tattgacaat gatcacaagg atcgttgagg tgatcatagc tacgataaaa ccaataatca
     8581 cggcattcgg acgataattg aataggatcg gtacatcaaa agctggtttg gctccaggga
     8641 cgactttttc agcaatccct tggaaagctg gaataatttg gttgatcaat aggcggaccc
     8701 cataaagcat gatcaagagt cctgcgccaa agttcaagcc tgcttgaaca gcaaacatga
     8761 ccagattccc accttctggg acaagaactg gtgcgataat tcctacaaca atattcatca
     8821 aaaacatcac caaggcacct gtaatggaaa tctcacggaa aaaggacagg ttttctggca
     8881 cttttaaatc ttctgtcgat ttttctttgt ttcctactcg ttttgccacg aagccggaca
     8941 ccatcgataa gatccctgta ggatgtccaa tcaagaacga atcatcgcca gtggcatccc
     9001 aaacatattt ccgcatgatc caaggcataa atgaccaata acaagcagat aaaacagctc
     9061 ctaaaatcaa taatggaatc cccgacattc caccttcaac cccaccagca acgatcacaa
     9121 atgggaacca ccataacata tgacccgtca agaaaatcgt cttaactggt gtaaaacgag
     9181 caatcaataa gtgcaagatc aatccaaaaa acatagctaa cccaacagtt cccccgtatt
     9241 ctgctgtgaa ggtcacatca gaaagaccat cggctacttg aacccccaac tgtgtctgaa
     9301 ccgctgtgtt gattggtgcg atcgttcccg tcagcatatt gacccctgcg gtcaatgcta
     9361 ccatcccaaa ggctgctgtc aacgagcctt tcaccacttc ggcaaaaggt tttcgttgca
     9421 cgatcaaccc gatcattgca atcagtccga gcaatactgg cggattcttt aaaatatgat
     9481 ctgtgacaaa actcatgatt atccctctct ttcagctaaa aacttttcca tttgcatttt
     9541 gacttcagcg acatccatat attttttgat gggatagaca ggtgctttga cggaagcttg
     9601 caaatcattc aataattcgt ggcttgtaaa ataaacatct gcaggagttg cttttgcagt
     9661 agacaaatcc gtattctcag cagtcgcctt cagccccagc tccttaatca ccttatcgat
     9721 cgtcatcttt aacaccattg aacttccaac accaaatcca cacacagcta aaattttcat
     9781 cttcttttcc tcctattgga ataattggca aatctgttct ttgtcaccat tcaaaataag
     9841 ttgatacttt tcctgatcca taaacacttc catgatttcc tgcaaacttt ccaaatgact
     9901 ttgattgtct tttgcagcta aaacaaggaa aatacggacc gacttgccag ctaaatcgat
     9961 ttcgttttta gtcactagca aactcatccc ttgatgaaag acaccttccc ccggacgagc
    10021 atgcatcaat gcaaagtaat cagccaatac catatacggg ccattctcct caacgctatg
    10081 gatcatatta gcgacatatt gttgatcgat ggctttttca tttaatagcg gctgactcgc
    10141 caattgaact gcttcttgcc aagtgaggat ttgattcgtg gtctcgatat gatcctcagg
    10201 cagtaatttt tttaaattga tcatgatttt acctccaaca tttttgaaat gaaccattct
    10261 ttgtcttcga tcccttgcat gatcaacttc atgacaggtt ctacttcttg ggcctcatct
    10321 tctcgtatca ttagtaacac gatgatatcg actttttggt tgccccaatc aacaggacga
    10381 tccaaaatca atacagaaaa ggtcgttttt gaaacaaact caggattgcc atgaggtatg
    10441 gcaaaaccgt tatctaaata ggtcgactgc aatgcttctc gttctaagac ggaacgctga
    10501 aagtccgtac gctgattttc cgacgaaaag tcattttgac tgatcagaaa atccagtgct
    10561 tgttctttgg tctcaaatcg ttcaaaataa atcttcggtg caatcaacat ccgtgtatct
    10621 tgcaggattt tcaccttatc cgcaatcggt tcattatcaa taatcaaatc gatatagtga
    10681 ttcatgatcc gcttcatgtc tcgattggtc accatcggcg agatgcggac gactggtttg
    10741 gaaacatcag gtaaatcgat cgtggaaata atgaaatcga tggcagaaat atccatctct
    10801 ttgagcttag cgacagaagc ggtttcaata ctatcgacat taggcaatat tcgacgaatc
    10861 ttggcagaaa taaatgaact ggtgccaaca ccgttcggac aaacaaagac gattttattc
    10921 aattgccgca tccgttctac tgcagcttga aagtgaatcg aaacaaaccc gatttcatcc
    10981 tcagatattc caatatggta ggcctttaga aagtcgtcca aagagaacca aacgacccca
    11041 tacaacgccg gataatgctg tttgatctca tctagcaatg gattgactac agtagtttgg
    11101 gcctcaatgc gtaaaaacag ctggtacaaa tgaatcgtca aatcttcttc taagcgacta
    11161 tcctgtgtca aatcgatctg gatgccttca ctgactttct gaatcagatt tctgactttc
    11221 cctcgaaaag agaacggaat cgattcgttc ttcataaaaa aacgtaagcc actacctaca
    11281 atgagatagg ttaggtactg aacttctttc gatgaaaaat ggtagatttc ttgttcttca
    11341 atttctttca acaattcata aatcaacgga tacttgtcaa attccaaaaa caattttcca
    11401 aattggcgct tctcttctat atccagctga tttgcctgat tcgagcgctc gacaatcagc
    11461 aagatcgaaa caacgatgct ttggatgtag ctttctggga tctccatttg cttttcggtg
    11521 atcgaatggc gaaaagccgc atttatcttt tcaaataatg acacatttgt cagaacagaa
    11581 aggttctccc catgctccac agtaaggaga atgaatcgct tcaacattcg ttggatttgt
    11641 gactcgcttc cttgaaaata agtgcctgaa ttatcaaaac gcaacagtaa attttctttg
    11701 acaaacagtg tttttagata agcgatatcc ttggcgatac tgctcctaga aacataatac
    11761 tcatcagaaa gttgctggta actcaagtgc tccccattga tcagccgctt aaaaatttgc
    11821 atgcgtcggt cttcgatcaa agaataattg tttttcattt gctcaccctc tacctttatt
    11881 ctagtgatac cttaacaaat atgaagacca attgttagat taaaaaatga ttaaaaggtg
    11941 tgcacaccct ttcccttgat agcaatatcg gtcgtttgtt ccgcaaaatc gcttcaagca
    12001 agccaaactt ttctcatctt cctcgttttc tcttataata aatgtaaaca gtcaaatgag
    12061 gaggaaacaa aatgaaatgg cgtgggggta gacaaagttc caatgtggaa gaccgctctg
    12121 gtcgttccgc aggttcagga ggaaaaataa tggtcggcgg tggtatcggt acgattgtta
    12181 tcgcagtcct cttttttcta ttcagtgggg gtgatttaaa ctccgtcaca gatattttag
    12241 gaggcggaca agccgctaat tatcaaacag aaaatgtggc ttcagacgag tatacagagg
    12301 aaaaagaatt ctccgctgtc gtcttcggcc atttggaaga ttattggaat ggtgtttttg
    12361 aagcagaaca gactacgtat caagatccta aactggtttt atttacagga agtgttcaat
    12421 cggcttgtgg gaatgcaact tctcaagttg gtccctttta ttgccctgcc gatcaaaatg
    12481 tttatctgga tctcagcttt gcaacagaac tttctcaaaa gtatggggcc agcggagatt
    12541 ttgccatggc ctatgtcatt gctcatgaag ttggtcatca cgtacaaaac gaacttggta
    12601 ttacacagca actcgataag atccgccaac aggtttctga aaaagaatac aatcaatata
    12661 gtgttcgttt ggaactacaa gcggattatt tagccggtac atttgccaag tatctacaag
    12721 gaaaaactta taacggccaa ccgattttag aagctggtga tatcgaagag gctatcacag
    12781 ctgctaatgc cattggtgat gatacgttac aaaaggaata ccaaggatat gtcgtaccag
    12841 atagttttac tcatgggact tcaaaacaac gagttgaatg gttcaaccgg gggttcaaat
    12901 atggtgattt ggagcatgga gacaccttca atgaaaaaaa ccttgatttg gtctacggat
    12961 cataacaaaa acgttccttt ttagtcactc ttttttcaca tttgggcggt atagtttatt
    13021 cataccaaca aaccctaact ttttcatttt actccttgcc tgccagtccc cgctggcagg
    13081 cccttttttt tcatggtgct attttagttt tttttaacct ttggcctgtt tttttcttta
    13141 aaacgctaag ggttgatcac aattttttca tattttccgg ttactatata aataacccaa
    13201 caaacaaccc aactttttca tttttactcc ttgtctgtca gtccccgctg acagacttat
    13261 tttttttctt gccaaaattc tcaaaatgcg ctaaggttat ttatgcgcgg aagtggcgga
    13321 atggcagacg cgcacgcttc aggcgcgtgt gagtatatct cgtgtgggtt caactcccat
    13381 cttccgcatt ttgagcagat catcttggat gatctgctct tttttatgtg tttcttttgt
    13441 ctaaataaag aaaaaagctt aagctatata aattgagtta aattgtattc gtttttcctc
    13501 ctataggcgt atactaaaga aaacacttga ggagtgggga acatggaaac agaacgccaa
    13561 atccgtgaat tggaaaaagc gatgaatacg acagacaacc gccggttgta cgagcgtttc
    13621 ctggcagtca agttggttct tgaaggccac acccggaaag aagccgggca caccatcggc
    13681 cgcgacgagc atactgtcag ccactatgtg aaaaactact gccataatgg aatccaaggt
    13741 ttagtctcca aaaaacaaag cggacgacct tcacggctga cagatgaaca ggaagcggaa
    13801 ctggtccaaa tcgtcgccta ccacactccg gaggaagtag gtttcaaatc ccgtgccaat
    13861 tggacattgg cgattgtgtg tgacctgatt ctccgggaat ggagttatgg atatacgcaa
    13921 aaaggcgtgg cggacctaca taactttccc cctagatatt acgaatatcc agggggtgtt
    13981 attgcatgcg taacagccta tgctaattta caacatagat cgaagtggct tagctctaaa
    14041 aaataagccg catctttgtc agatacgact gtttagcatt gctcattcaa ttagttgatg
    14101 attttttcat caaatttcag atatagttga atatttcctt tgttcagctt atcaaatgtc
    14161 actttatcaa tgaatgttcg atagatttcc ttgttctcac tcttgaaacg attggctaac
    14221 aacttttcaa cacttgctcc tttgtttcga aatggaggct actcatcaaa aagaataaat
    14281 tcacggtact ttctagagag tattgatagc aaaatattta atccaatcat tgaacgactt
    14341 ccttcaatta aattgcttgg aagctctctt gtctcataat ataacaaaaa gtcggaatgc
    14401 tcgctgagga aattttttcc cagctcagta actccgcaaa gtggaattcg gttcatattc
    14461 aaatttttga ttgttgattt gatgcttggt gtctcaccgc ttagagacgt aacgatcaca
    14521 aaatcatctt ttgtcaaagt atggcttaac atttcaaagt ttgtttcacc tgaaattaac
    14581 atggctggtc gtcccgataa aatcaaatcg tttacaaagt cttttgaaaa gttattttgc
    14641 gtaaagcctg tcgcatagac aactactgtt tttgccgctt ttagctgttc taatatcggt
    14701 tggaaatttg tttggtccgc taattgaaaa gttcggttga tttcatcttt caatagtgcg
    14761 ataagatctg tcggtggaat aaccgattga acgatgctct gttcaagaga atatttcatt
    14821 tcagaatacc cacgaaatcc caattttttt gctaaacgta agacagaact tttagaagac
    14881 aataattgtt ctcccaattc tacaatggac atgtgaacta cttgagcttt atgcgtacga
    14941 ataaaaaaaa ccatatcttt ttcattatct gagagcaaat caaagtttag tcgaacattt
    15001 tgttcaaaat ccattttctc acctctcata tatacagaag gaatttcgga taaaacttgc
    15061 gctgttttat ccgaaattct ttcttattgc ccaaattcta acacagtttt tacgtgcttg
    15121 ctatatatga taaacaagtg cttcgtaagg tcgtaatata aacttatctt ccaattcaga
    15181 tggtgattgc tcataatttg atagaagaag aacccctttt cgaccttgca atgatttagg
    15241 taattcatag ggaacagact cttttgaaaa agaacagatc accactaata gttcattttt
    15301 atactcacgt atataaccaa acactgctga atcattctca ttgatcaatt tatacgtacc
    15361 atgctgaatg attggatagt ttttacgcag tgaaatcagt ttttgataat gataaaaaac
    15421 tgaatttgga ttttttaaag catcttctac attgatttgt tgattatctg gcgaaaaaga
    15481 gatccaagga tcactgttcg agaatccata cccttttgaa tgatcccaag gcattggtgt
    15541 tcgtgcgtta tcacgagatt tcaaataaat agcacgtttg atgtactcat cactctcgcc
    15601 ttcttctttc atctttttat aaaaatagtt tgtttcaatg tcttgatact catcaattga
    15661 agaaaatgga acatttttca ttccaatttc ttccccttga aaaataaatg gggttccttt
    15721 catcatgtga agtagtgttc ctaacatctt actagactct atccgatatt cgttttcatc
    15781 accaaatcta gtaacagctc gcggttgatc atggttactc cagtaaagag aattccaacc
    15841 atctgaaagc gcttcttgcc actcacttaa cgtagctctt aaatcgctaa gcttaaacct
    15901 tacatctgaa aactttccat actttccata atcaagatgc atatgatcaa aatgaaaaac
    15961 catatttaat tctttacgct caggaaacgt ataaagctgt gcctgtctgc tatttgtgtt
    16021 cggtgtttca ccgacagtta agctgtcata tttactcaaa acttctcgat tcatctcttg
    16081 taaaaattca tgcactcgcg gaccgttgga tgctccgaca taataagact ttgtataggg
    16141 catgctattt ggagcatcag gataatcagg atgcttgcta atcaagctga taacatccat
    16201 tcggaatcca tcaattccaa gatcaaacca ttttttcatc attttataaa tagactgacg
    16261 aactttcggg ttctcccagt ttaaatctgg ttgttctttt gcaaaacaat gtaagtaata
    16321 ctgctttcta ctctcaacat actcccaagc cgacccgcca aatgtcgatc cccaattcgt
    16381 aggtgcactc ccatcttctt tgggatcttt ccaaatataa taatcagaat atggattttc
    16441 tttatttttt tgtgattcta taaaccaagc gtgctgatta cttgtatgat tgacgacaag
    16501 gtctaaaata actcgaatat tcagttgatg cgcctgttcc aataatctct taaaatcttc
    16561 taacgttcca taattttctg aaattttctc atagtctcga atatcatatc cgttatcaat
    16621 tagcggagag tcaaaaattg gagttagcca gataacatca acccctaact ttgctaaata
    16681 aggtaatttt tcaacgatcc ccataagatc tccgatgcca tcattgtttg aatccttgaa
    16741 actttgagga tacacttggt agacaacact acttttccac cattcattca tccgttactc
    16801 atcctcctca tcaacgacta tattttttaa gtaatcattg acataaacca attggtcttg
    16861 tgcttcttgg attgcctcac ggatgtggtt gtctgtcaat cttttgttta atgtattcaa
    16921 taaaattgtc aataccacag gtagattcat cccggtaatc agatagacat taggttgatt
    16981 gatatatttt ataaatgctt gattgactga accacctagc atatcagtaa aaatcaatat
    17041 atcagtatcc tcattgtctc ttaccattac ttcgttaatc ttttcttcta taggtgagtt
    17101 atcaatataa cctgcgattg cagttatttc accaacattc ggtaaaatat attggacagt
    17161 atctttcaat cctacagcta aattatgatg actagcaaaa ataatttttt tcattcttta
    17221 ttctccttaa acctttaaaa taccgaaaaa tgatagaatc aatgcgaata tgatcactga
    17281 aaaaataatt tgaatgaccg tcattttttt tgaagctatc aatttataaa caataattgt
    17341 taaaactgca ggtaagagtg ctggcatgat tttatcaaga atttctgttt gaataggtaa
    17401 ttttactttt ccagattgaa aaaccactgc aacattcatt tttacaacag atggaatcaa
    17461 agctccaacg actgtcaaac ccataattga agcagattct gtaaatatcg ataatttact
    17521 tcctaaagca gtcaccaact ttaaccctga agtataccca atcgaaaaaa gtttaaatcg
    17581 gaagaacaaa aagaatagat tcaaacataa ccaaataatg gcaccagtcg gatttccttc
    17641 tagacccata taagctgcaa tcgagcccat aatcgttgga taaaggaccc aaaccaacgt
    17701 gtctcccaca ccagctaaag gacccatcat cccagtctta aaactttgaa ctgcatctaa
    17761 tgaactgact ccatcttttt cttccattgc taagctggca cctaataata aattcgccat
    17821 ccaagtcgtt gtattgaaat atttaaaatg gttattcaga gaagctctgt agtcttcatc
    17881 atttggataa atttttctta gtgctttttc aagtccataa acaactgccg gaccttgttg
    17941 attttcgtaa ttgaagatat tacatgccat aaacatatag cgaaatgctg ctctacgtat
    18001 atctttcttt gataatttat ttccttcatt attcatcaaa atcatctcct tcatcgacta
    18061 tattggcagc tacaggtgat gccgtctctg ctttttttat ttctgtcgtg aaaaagtaat
    18121 acgctaaagc aaagccgacg attgaaattc ccaaaatagg catgtttaaa taggcggaaa
    18181 gtacaaaacc aatcaagagc atcgtcatat attttttcac cggcataact tgtagaagta
    18241 atgctattcc gactacaggc aacatgcttc cagcaatcga taatccatct gtaaaccact
    18301 taggcacaaa atctaaaatc aatcgaacaa ttctatcacc aaaaacaaca cacaatgcag
    18361 ttggaatggc tgtagataat ccgaaaatca ctggtcctaa ccacaaaatc tgattcattt
    18421 tcttaaaatc cattttattt gctgcctttt gtgccgcatg agtaacatat gtgttcaata
    18481 ttttagccaa cacatctaat tgaattccca gcatcccaac aggaacacct actgctaatg
    18541 caacagcttt tgcttcttca atattatttg tggtacggat ggctacgtag atacctacaa
    18601 gagcagctaa accccagtta ggaacggacg atcccccaat attcgctaca cccaaagcca
    18661 ttaattgaaa cgtaccgcca ataaccattg cggttgtcat atctcccata atcagccctg
    18721 caaacaaagc aatcatgatg ggaaaccatg ttgtaatgac caatccttgt tgatctaata
    18781 caacccacac tgctaaaata ataattaaaa ttccttggat aatttccatt ccaattatct
    18841 cctttatttt attctcacat ctaatggttc ttcaggatct gaaggaacaa aatgaaaagt
    18901 aactggtatt cccatagcct ctaatttttt taaatcagca atttcttgtt catcaactga
    18961 aaccattttt tttactgttt gtttcccttc accatcaaac atgatcccga tattcacttt
    19021 tggtatatcg ataccgttct cagctagttt tactaatgta ctcggttctt ttacgatcat
    19081 aagcacatta tgactgtcat acttgccaag tttgaaatta ataatagctg tatctgtatc
    19141 aataattgac atccccgttc cagctggctt actcattcgc attgctgctt tttgtgtgtc
    19201 atccttactg acttgatcat ctacgaccat caatcgtttt gcttttgtcg cacctgccca
    19261 ctgtgttacc acaataccgt gaatcaatcg ttgatcgatt cttgctaata ctaatcccat
    19321 atctatcgtc tccttttttg ttatcgttta catcccaaag tatatctacg ccttgttact
    19381 ttaacaatag ttttagaagt atcagggaaa cagtcgttat cttaggaaat agtttcttta
    19441 ttaagagaaa tttcccacaa aaaaatagcc caaccatcga ttagatgact gagctcccta
    19501 aatttacgct atcttttatt actatttact tcttttttat cgcaatccaa atttcgccat
    19561 cacaatccgt caactctttt gagaaatcag taaaggagag ttctgatgct tgaaccaact
    19621 catattctga agttggcaac cactccgcgt atattgaccc gctgacatcc ctaaaaaagg
    19681 tgcgaggtgg agaatatttt tgatacaact atccttattt atgatatgga gcccccgcat
    19741 tgatccggaa ggcacggtag atttgctcga caaggatcag acgcatcaat tgatgtggat
    19801 acgtcatttt tccaaaagag atgtgagcat tgcttctttt catgacggct tcgctgagtc
    19861 ctaaagagcc gccgataaca aaaacaaggt ggctttttcc ttggatacct aattggtcga
    19921 tttgtttagc aaaagattcg cttgtgggat ttttcccttc aattgctaag gcatagacgt
    19981 attcttgttc tttgattttc gctaagatcc gttccccttc tttttctttt acttgtctca
    20041 tttgcgcttc gcttaaattc tccggggctt tttcgtcagg aacttcgatc aattcgattt
    20101 ttgcataggc attcagccgt ttgacatact cattgatacc ttggatcagg tatttttctt
    20161 ttaattttcc tactgagata attttgatat tcatgttttc tctcctttgg tcttcatgct
    20221 cactttgttt attataatca attttttcgt ctatatgttt attttggcta tttttgttaa
    20281 aaagatttcc ccaacagtta tgcacaacat ccacaggttt atccacagaa aacagtgtta
    20341 ttttacctta tttcatcaca tttattaggc aaacatacgt tttataagaa tttatccact
    20401 ttatacacag gctgtggata tgttttatcc cttatttttc caatcctatc cgcgtagttt
    20461 tacacagttt ctgtggaaaa aagcaaatca aatattttca tctgaaaaca atggtttttc
    20521 ctaaagtttt tcataaaaag ttcacagtct caaagtagac taaagtcaac aaataagagt
    20581 atactaacag tagataaatg aatgtaattg gaggaggaac acatggatag aaaaaatgtg
    20641 acaccaaaga tgaaaaaaaa taaaaataat agcctctggc gtaagttagg tctagggctt
    20701 gttggaggga tcgttggagg attactgaca gctggtattt tttatgcagc gatgggcact
    20761 ggcaacaata gttctactac aagtggcaat caaaattctg ctggtgaaac agttgtggaa
    20821 aacgtcaaag tcaatgtaga ttctgatatc accaaagcag tagataaagt tcaagacgct
    20881 gtcgtttctg ttatcaactt gcaaagtcaa aaccaatcaa gcggttttgg cggactattc
    20941 gggcagcaag aagaaagtag cagctcagat gatagtaatt tggaagctta tagtgaagga
    21001 agcggcgtga tctacaaaaa agacgggaat actgcttatg tagtcacaaa caaccacgta
    21061 gtagatggcc agcaaggtct tgaagtcttg atgaaagacg gtacgaaggt aaaagctgaa
    21121 ttagttggaa cagatgctta ttcagattta gcggtgttga agatcaattc agacaaagtc
    21181 gaaacagttg cctcttttgg tgactcaagt gctctgaagg ttggtgagcc tgcgattgcg
    21241 attggttccc cattaggatc tgaatatgcg aattctgtca cttcaggaat catttcttcc
    21301 ttgaaccgac aagtaacaag tacaaatgaa tcgaacgaaa cagtcaatat caatgcgatc
    21361 caaacagatg cagcaatcaa ccctggaaat tctggtggtc ctttagtgaa tatcgaagga
    21421 caagttatcg gtatcaactc aagtaagatc gcaagtactt ctgaatctac ttctaatgta
    21481 agcgtagaag gaatgggatt tgctattcca agtaacgatg tagtgaatat catcaatcaa
    21541 ctggaaaaag acggaaaagt gacacgtcca gctctaggta tcacaatggt cgacttatct
    21601 gccgtttcta ctcagcaaca agaacaaatc ttgaagattc cttcatctgt aactaacggt
    21661 gtcatcgtga caaatgtcca aacagctaca ccggctgaaa aagctggatt gaaaaaatac
    21721 gacgtcatta ctaagatcga tgacaaagaa gtttcttctg gtgtagaact tcaatctgct
    21781 ttgtataaga aaaaagtggg agattctgtc aaaatcactt actatgaagg ctcgaaagaa
    21841 aaaacaacta ctgttgaatt gacgatcgat caatcggcat taaaacaaaa tcaaaatagt
    21901 gaaaattaat aaaaaagagg ttaggttg
//
LOCUS       DAXLPI010000044        21366 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXLPI010000044 DAXLPI010000000
VERSION     DAXLPI010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.044"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(74..376)
                     /locus_tag="ACF3T5_001383"
     CDS             complement(74..376)
                     /locus_tag="ACF3T5_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFD3631997.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(402..833)
                     /gene="ruvX"
                     /locus_tag="ACF3T5_001384"
     CDS             complement(402..833)
                     /gene="ruvX"
                     /locus_tag="ACF3T5_001384"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFD3631998.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(830..1096)
                     /locus_tag="ACF3T5_001385"
     CDS             complement(830..1096)
                     /locus_tag="ACF3T5_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFD3631999.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1350..2075)
                     /locus_tag="ACF3T5_001386"
     CDS             complement(1350..2075)
                     /locus_tag="ACF3T5_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFD3632000.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2441..2511)
                     /locus_tag="ACF3T5_001387"
     tRNA            complement(2441..2511)
                     /locus_tag="ACF3T5_001387"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2477..2479),aa:Gly,seq:ccc)
     gene            2628..3380
                     /locus_tag="ACF3T5_001388"
     CDS             2628..3380
                     /locus_tag="ACF3T5_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFD3632001.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3396..3875
                     /locus_tag="ACF3T5_001389"
     CDS             3396..3875
                     /locus_tag="ACF3T5_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFD3632002.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4126..5706)
                     /locus_tag="ACF3T5_001390"
     CDS             complement(4126..5706)
                     /locus_tag="ACF3T5_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFD3632003.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5699..6442)
                     /locus_tag="ACF3T5_001391"
     CDS             complement(5699..6442)
                     /locus_tag="ACF3T5_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632004.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6618..7394)
                     /locus_tag="ACF3T5_001392"
     CDS             complement(6618..7394)
                     /locus_tag="ACF3T5_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFD3632005.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7642..8478)
                     /locus_tag="ACF3T5_001393"
     CDS             complement(7642..8478)
                     /locus_tag="ACF3T5_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFD3632006.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8489..9418)
                     /locus_tag="ACF3T5_001394"
     CDS             complement(8489..9418)
                     /locus_tag="ACF3T5_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFD3632007.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9610..9963
                     /locus_tag="ACF3T5_001395"
     CDS             9610..9963
                     /locus_tag="ACF3T5_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFD3632008.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10037..10693)
                     /locus_tag="ACF3T5_001396"
     CDS             complement(10037..10693)
                     /locus_tag="ACF3T5_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFD3632009.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10919..12853
                     /locus_tag="ACF3T5_001397"
     CDS             10919..12853
                     /locus_tag="ACF3T5_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFD3632010.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12939..14162)
                     /locus_tag="ACF3T5_001398"
     CDS             complement(12939..14162)
                     /locus_tag="ACF3T5_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFD3632011.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14156..16027)
                     /locus_tag="ACF3T5_001399"
     CDS             complement(14156..16027)
                     /locus_tag="ACF3T5_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFD3632012.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            complement(16048..17166)
                     /locus_tag="ACF3T5_001400"
     CDS             complement(16048..17166)
                     /locus_tag="ACF3T5_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFD3632013.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17249..18082)
                     /locus_tag="ACF3T5_001401"
     CDS             complement(17249..18082)
                     /locus_tag="ACF3T5_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632014.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18075..18992)
                     /locus_tag="ACF3T5_001402"
     CDS             complement(18075..18992)
                     /locus_tag="ACF3T5_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632015.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19219..20343)
                     /locus_tag="ACF3T5_001403"
     CDS             complement(19219..20343)
                     /locus_tag="ACF3T5_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFD3632016.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20526..>21366
                     /locus_tag="ACF3T5_001404"
     CDS             20526..>21366
                     /locus_tag="ACF3T5_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632017.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIH"
BASE COUNT         6644 a         4239 c         3513 g         6970 t
ORIGIN      
        1 gtgctaaacg aaaaaaccgg gctctacttt tcagagagtc cagcttttct tacaccttat
       61 cgattctttt gttctattct tcttccgcca tgaatgtatt gaatacttct tcgatcatat
      121 cccattctgc gtctgtttcg atttgttcta actcgccttc tgttccatct tcgttttcga
      181 tgtatgcata agcttgtaat tctacatctt catcttctga aacaccagct ggatataaaa
      241 gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg
      301 tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat
      361 ggttatgttc tgtcataaga ttacctcttt tctttttatt attaaaataa ttgcttacct
      421 gagccgtcta aataattttg taaaatcatc acagctgcta atttatcgat gacttttttt
      481 cgtttcgctc ttgaagtatt tgcctgctct actagcatac gttctgcctg aacagtggtt
      541 agtcgttcat cttgataatc aaccggcaag gagaatagtt ctttgatctt ctctccatat
      601 gacatggaag cttctgccct tggtccgatg gaattgttca tattcttagg taatccaact
      661 acaaaacggt ctacttcgta ctgcgccact aattccttca accgatcaaa accaaactct
      721 tcttctgcct cattgattcg aatgatttct actccttgag ctgtccagcc aaagggatcg
      781 ctcacagcaa cgcctactgt cttagaaccg acatccaagc ccattactct cataaattca
      841 ctccatgatt tgagaggtag tctttgacaa ttacttccat gatttcatcg cgttcgtgac
      901 ggcgaatcaa atttcgtgca tcacgataac gaggaatgta ggccgggtct ccagaaagca
      961 agtagccgac aatctgattg attggattgt agcctttttc ttccaaagcc cgataaacaa
     1021 tcgctaatgt ttcgctcact tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt
     1081 catctgtaaa acccatgatt ctacacctct ttcccgcaag gtaattccag cttaccttct
     1141 acacccttat tttactcgaa gaaagggaaa actacaaaca aatattccaa acttctgaat
     1201 aaattaagaa acacgatttg tctgattttt ccttttttct gtggcttttt caaaagccct
     1261 ctttccataa agtatacgct atatttcttt gaaagtatgt ttttacacaa ttttttcata
     1321 aagttcataa aaaattcgaa ttgctgctac taatttttcg ttttcgttgc ttgccctgtc
     1381 tcataattga tggtgtagcc atcttgaaca ttgtagataa agacattgaa ttcaattttt
     1441 ttgtcttcaa tgctttctgc ttccaactgc acacctcttg ctaccagttc atttccctcg
     1501 aaatgaggag tgacacgata gcgtacatga tgatttgttt ccttgatata tgccgcaata
     1561 tcattttcgt gggcaagcat gtacggagca ttcaacgaac gtgtccctgt cattaagttt
     1621 ttaatgttat tattttgacc agtcaactgg aaaccaatca aatggctgcg gttgtataac
     1681 cattgtccgt cacttaattt cttttgtttc caacctgtcg gatcaatata caaaggttct
     1741 ctcttttcag taggaaacaa gtcttttccc aacatcgcat tggcttgtcc tactctgttc
     1801 aatttgtcta tatttgaata gctttcccat gtccctttgg aaagactaag atcctctttt
     1861 gtaaactccg gctgattccc attgatttct atttcttgat gttttccatc atattctggc
     1921 acatccgcta cttgagaagc ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag
     1981 atatctgaat ctttttgtgt ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg
     2041 atccccacga tcgccttggt tgtttttttg ctcatttctt tactccttta ctaacagttc
     2101 cctaaatctt aacatatgtt cccctataaa taaatactgg aaagaaatag aaacagtaaa
     2161 aaaataaaac cagtttttag acggcttaag aatacaaaaa aagagacgga ttcatcgtct
     2221 ctactcaatc acaaaacact atttacaagg tatttattat cattcaaaaa atttttctta
     2281 atactcaaaa ctaaacggct tctgtacgac ctcttggtaa acagtgttca aaagcttgca
     2341 tctgcggtaa taagtccaac caagtcaaaa acataagaag cttttttgct tgactattct
     2401 tattacttga tgcagggcag ctttttcaca gcctctttta agcgggtgac gggaatcgaa
     2461 cccgcgacat cagcttggga agctgggatt ttaccactaa actacacccg cgatacaaaa
     2521 acagtatacg cctctttcac tccttcctca agttttttta ttctttacgc ttccgatatg
     2581 ttttgtagta aaataaaact aatcaatcaa ttattggaga agatgctatg acgggaaaag
     2641 aattagatct gttgctggac acttgtttac tcgctgggaa aatcatgatg gaaagcaatg
     2701 cggagatgta tcgggtagaa gatacgatga gtcgaattgc tttagcttct ggaaatttcc
     2761 gtttggtcag ctatgtcact caaaccggtc tctttattgg tcttgatcgt acctcgacca
     2821 tccgaatgga acaaattacc aaccgttcga ttaatttaga aaaagtagta aaagtaaatg
     2881 atctttcacg taaatatgtt gcttcagagc taacattaga agaactttac tgcgaactgc
     2941 aagcaatcga ccgagaccgt tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag
     3001 tgatcagtgg aacaattatg gtgttatttg gcggaaagat gaccgattta cctgtgactt
     3061 tagtcattgg aggattggga tacgcagctt atctatatag tttgaaattt tttagaatca
     3121 aatttttatc ggaattttta tcttctttat tgattggcat agccgctatc tacagcgtac
     3181 gttttggctt aggaagcaat caggatctaa tcattatcgg atgtgtgatg cctttagttc
     3241 ctggagttca aattacgaac gctatccgtg atttgctagc tggacattat gtctcaggag
     3301 tttctcgcgg aacagaagcg atgatgactg ctacgatgat cggttttgcg attgctttta
     3361 ttttccagct tttttattag gaaaggaaca caagtatggg gacgatattt attcagtttt
     3421 cttttagttt tttagcgact gcggcatttg ccgttattac aaatgtacct aggcgttcat
     3481 tgatttgttg tggtctttct ggtgcttttg gctggatgat ctactggatc tgtgttcaaa
     3541 taggcggatc tgctgctttt ggttcattgc ttggttcttt aggagtagca ttcatcagcg
     3601 atcttttctc taaacgtttg aaaatgcccg taacgatttt caatattccc gggatggttc
     3661 ctctagtccc tggtggttta gcttatcaag cggttcgaaa tctcgttact gaatcctatc
     3721 gagaagcggc tcattatgcg gtccaagcaa ttatgattgc tggagcaatc gctttaggtc
     3781 ttgtattatc agaagtcttt aaccataaca tccgtaattt caaagtaaag cgtgacgcaa
     3841 tcatgatgaa acgaaaaaaa cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg
     3901 tgaaagagac gttcagcctt gagcgatgac aaaatatctc acaaacattg cgacaagtaa
     3961 ccgcaggagc aataagaagt aattatgagt attaaaaaaa gttttgtaaa aatagcttct
     4021 cacattttta cataaaattg acttaatact gagaaactga cttttgaaca ccctctatct
     4081 gtaaaaaagg ggctgcgaca tgatttttgt cacggcccct cttctttatt caaatttttt
     4141 ccaaaacgtt cgttgataat aaacgatcca tagagcggaa agtattagaa ctaaaccgat
     4201 cacaggaata ttcactaacc aaaatggata aaacaaagcc aacattccgt ataagacgat
     4261 aagaataata ctgatgatca atgaacccat catagttgcg actaaaccca aacttcctga
     4321 acctctgtta aataattggt taatattggt ccagttcagc agcagcaacc gataatctct
     4381 agcgaaataa cgtagtgaca ataagtagca gccgaataaa gaaccaaata gcaagctagg
     4441 gataaaaaac aaaggcagct gaaaagacag tccgcttaat aatgcgatcc caccagttaa
     4501 aaaactctgc aatatccatc cgaatctgaa cttttctttt aaatattggt tcatagagat
     4561 tggcaaagaa cggataaaca gaaaattttc ttgatccaaa gaaataagat tgctgataaa
     4621 agaagtctga ttaactgtta agaaagccaa tgcgatccct accaggaaaa caacaccgat
     4681 taacttatta tctaaatcag ttaattttat ttcgcctcct agggcaaaag caagaataaa
     4741 aatgattgga gttaacaagg aagaagaaag aacttgcata atcaaattag gttcttttac
     4801 taattgagca ttgtaactaa ataaaagctg atgcacattt tggttctttt tatgggttct
     4861 ttttattttg aactcactgt ttgaatcagt cagttgctct gtaagctttg gtactatcag
     4921 ctgttttaca gcaaggaaaa atcccagagc cgtgatagct agtattcccc aacccaataa
     4981 accttgttga gaaagtggct gactggcagc atcatagaat ggcaataata tcgaaatcgg
     5041 atgccgatct gttaactcat tttcaatagt ccccgtttga tgattcatcc acatgattcc
     5101 agcaaatgcg atcagcatcg agctgccaag caagatacta gaaactgttt ttctatgttt
     5161 tttaaacact gctgttcgag ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat
     5221 aatcaaaaat agagaaaaca aaaggattga gataatgata gtaagcaaaa tccctagatc
     5281 agctcgccat ccggttaaaa taaacatgac tagaagcgga aaaacaaaag gaatgatggt
     5341 caaagcaact accaaaatct ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa
     5401 aaatacttgc aggtcatttc cttcaaaaaa gatattataa atgactgata tactttgagt
     5461 taaacctaac ataccaaaaa gtgccacgta ataagtaaag aaacctggca atttaacaaa
     5521 gttcatagca aacatagcaa atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt
     5581 ttgagacaaa agatagatag ttaatcgctt tccctttttt cctttctctc ttgctttatt
     5641 cgtgagctgt ggattaacat ataaaagatt aactcgaatt aattctaata attgtcgctt
     5701 atacatacgg tccatctcct gtcgcttgcc ttccagccat tttcaaataa atcttttcta
     5761 gcgtttcatc aggtgagcca ttcaataatt cactaactgc cccctgataa atcaattcac
     5821 ctttcttcaa aatagccagt ttatcgcata actgttgtgc ggtatccaat acatgtgtag
     5881 aaaaaatgac tgtttttcct tttgcggcat gagccttcat catttctttc aaatcaaatg
     5941 ctgcttgagg atcaagtcct tgcaaaggtt catccaatac ccaaatatct ggatctggca
     6001 gcaacgtgcc gatcaaaatc gttttttgac gcattccatg tgaaaaactt gcgattgttt
     6061 cattttgatg actatacata tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt
     6121 tttgtgtgtc taattcatag gcagctgaaa tcaagtccca atattctcca gccgtcaatt
     6181 gtaaaaaaat atctggggaa tcaggcacat aaccgatttt ttgtttgata ctcaatcgat
     6241 tttccgtcaa tttcattcca tctactaaaa tcgttcccga tgatggttca ataatactaa
     6301 ctaaactttt aatcgtagtt gattttccgg caccattatg tcctaaaaat ccaaaaattt
     6361 ccccttgtct tatcgagaga gacaagtctt ttaatgcttg ttttttcccg taacttttac
     6421 ttacattgat gaattcaatc atttctgtac tccttttgtt tgttattact ttttattgta
     6481 cccttcgtta ttggtggagt aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg
     6541 actaggctgc tacttgggtt gttctcttct acaggaaaac actcttctaa caggtaagat
     6601 atgagtcttt gttttcttta aaatgattca ttttctatct tttcttgcat aaaattcttt
     6661 agtttatcaa tacttctctt tcccatctct agttgttctc catttgcaaa tgttactatt
     6721 tcgtttactc tgctaataga tgtaatgttt tcagtattaa tgatataagc ttttaaatct
     6781 ttacataaat actcatattc gagtgatttt ttatattttt taattgtgcc gtttacgaga
     6841 atctcttcat ctttcaatac taggcctatt ttatttttat aacctggcat gctcattaga
     6901 taaaggattt catcgtaatg gatatagtga tcgctattac cataacggac agttgtccac
     6961 ctttttttcg tagcatgttg aacaggatag ttttcttttt ctttcaataa gtaactttgg
     7021 atgctttttt tatttttttc ataagtgaat tcttttatca aataaccact aggcgtaatc
     7081 tttcgattaa taacagttat tgctttattc ggatgagacg ttagataaac aattttacac
     7141 ttttcattct tttctctaag catttcgcct agttcgatac cgctataata ggcgttcaaa
     7201 tcgatgtcta ggaaaaaaat atctgaggaa tagatttcat tatttttaat gaattcaaca
     7261 aattgtataa cgttcaatcc ctgaacgatc tcaaatttcc aaggacttaa taaaattctg
     7321 gattcttcca aaatttctac tagcattttt tcaaaaacca aactatcatc caaaataaat
     7381 atacgcgtta ccataaaaag tacctccact cgatatttta ctatcaaaat catttcatac
     7441 aacttttcaa catacatgtt aggaataaat tgtaattctc ttcacaatct tttccaacac
     7501 ctttgaaacc gatatcaaat actttcagga tgctgcttct aaaataaata tcaaacaaat
     7561 gaatacatca tgcaaaaaaa gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct
     7621 atgtttattt attttgttct tttaaaaaga acgatacaca tactgatttt tcggagcaga
     7681 gactttctca gcgtttgtaa caactacaga tgggatttca cgcttgaatg gttgataata
     7741 gtctgattgg atggttcctt ctattttata ccattcatca tttttaaccg ttactccttc
     7801 cggcatatgc gtcaacaaac caaagactcc tgaatctgcc acacaatgga taatgccgaa
     7861 tcggaacaag aagatatctg tattgctatc gttcggtgtt tggtaaacaa atccttcata
     7921 gctgattttt ttgccaatga attcgctagg gtaattataa attagctcca taacttctaa
     7981 atagttttca tctgtgatct tgatagtttg cttatcccca tacttttctt tcaactcatt
     8041 catttggtct aagtagtctg atttgttgaa ataaatactg gtatccggct ttaagtattg
     8101 tgtctgcatc tccggatccc ctactgattc tttacttact gggaaattaa atcctttggc
     8161 ttctacgatc gtagtgtcga gactaactgt tggaaacaac gtccccacga ttactggcaa
     8221 agctagcagt acataagcga ttccacgttg ataaggtttt gacaacccat ggtcatgatc
     8281 gtgctcgtgt gccttttctt ctgcttttcc gtttttgacc cacaaataca gctgaactaa
     8341 agccaaaaga gcagataaaa tcattgaaag aatagctaag tagcgataat ggacattgat
     8401 atattgatcg agtttgccag aaacttgcaa gtacatcatc aattcgaaat acccagataa
     8461 aatcaaaaac cgcagcatat tttccctcct aaataaataa tgtgaatcca ataacgctaa
     8521 gggcaatcag tccgatcagc gataaaataa agcgtcctgt aaaatatcgt ttcatcatca
     8581 gtagattttt gatatcgacc attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc
     8641 cgaacagact taacaaagaa gatcctataa acgcatctgc ttctgagcaa agagacatcg
     8701 taaaggccaa taacagcatg atgataatgg ctaaaatctt tgttgagccg agcgttagca
     8761 taattctagt tggtaaataa gtctgcatcg cagctgcgat taatccgcca atgatcaaat
     8821 aacgacctgt atcaaaaaat tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga
     8881 tcagctgatt ttttggttca ttcttctcac tttgttcttc ttcatggaca tgatgatggt
     8941 gattttctgc tactttagac gtgatttttt ctttcaatac agcgtctttt tgaaaatagg
     9001 caagccagac accaatcaca agggcaacaa aaaagctgcc tatcacccgc cagcttgcca
     9061 tctgccaaga attgccaaat gcgataaaag tggaaaaaat cacgattgga ttgatgatcg
     9121 gagcagtcag cataaaagca aacgctgtgt gaactggtac gcctttttta acaaattgat
     9181 gaacgatcgg tacgatgccg cattcacatg aaggaaagaa aaatcctaag atcgaaccag
     9241 taaaaataga gaagatacga ttttcaggaa gccatctctt cacacgttct ggggtcaaaa
     9301 atacttgtaa ggcgcctgag ataagacagc ctaataatac gaaaggtaat gcttcaataa
     9361 cgatcgataa gaaaatcgta ctcatctgca aaaccgaatt tggaataaaa gaaaacatga
     9421 gtttcctcct ttttatttct agttcattcg ctttgcaaat atacgctttt attcaaaaaa
     9481 aacaagttgc tctgtaaatt tttacacaac tcttattttt taccaaagca cttcaattga
     9541 ctttttctgt tattcccatt aaattaaggt ctcatacata tcacttctat cataaaaggg
     9601 agaataaaaa tgaaacaaac cactaacttg actgaagttc aagacatact gcaacagtca
     9661 tccgttgccg tcatttatct ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca
     9721 cgactagagg aacttctcac acattatgat attccggcac ttcatttgga tgcatttgaa
     9781 acaccagaag tagctagtaa atttgaagtg ctgactgcgc cagttgtcct gatttttcat
     9841 caaggaaaag aagtctttcg gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta
     9901 ctagatgaac tgacagcaga agatgattcc ctttcttatg aagaaatctt ccgaacaaaa
     9961 taatcgttgt ccaaacagaa aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt
    10021 caatcgcttt ttatttttat tctgctgtat tttcggaatg cgcatttgcg tcagaatgca
    10081 agaaatagat caaggtttgc aattcattcg tcaaatcaac attttggatc aatacatctg
    10141 acggagcatt caatcgagca gcggtaaagt tcaagattcc tttgattcct gcttccgtta
    10201 actgattgac tacttcttgt gctctttcag cgggtatcgt taaaattgct acttcgattt
    10261 gttggatctt gatttgttct gacatatctt ccattggata aacggggata ccgtctacga
    10321 tgcgcccaac gatttcctca ttcacatcaa aagcacaact gatacgtatg ctattacttt
    10381 gatggaattt gtattttagt aaagcacttc ccaagttccc tacaccgatc agcgcaacat
    10441 ttgtcaattg atcgtcgttt aatgtctttg caaaaaagtg cataagatct tctacatcat
    10501 aaccataccc acgttttcct aattcgccaa aataagaaaa atctcgacgg atcgttgcac
    10561 tatctacttg aacggcttca cttaattcag ttgaagacac ttttttcttt ccagcattat
    10621 ttaaaatcct taaatagcga taatacaaag gcagtctttt agctgtcgcc tttggaataa
    10681 tatgatcttt cacgaaatga cctccacgta ttgtctaatt acatctttga tttctccatc
    10741 atagcagtat ttataggaaa aaatcaattc cattgctcag atattgtgaa attttattct
    10801 ttattcattt atttatcatt tctgcaaatc tcgcagtata atggaattta tggtagacta
    10861 ctaattgaaa aatagttttt tatttgagga gaattcacaa agtagaaaag aggattccat
    10921 gatattatta caagccaatc agattgctcg ccattttggt gcagaagtct tatttgaaaa
    10981 catacattta gaaatcagta caggcgctcg tgtcgcttta gttggcagaa atggcgctgg
    11041 aaaatcgact ttattaaaga tcattgctgg aattgaagca cctgatgaag gaaagatcgc
    11101 taaaaataaa acagcgactc tcggatacct cgctcaggac acaggacttg attccaatga
    11161 aacagtttgg aacgagatgc tgaaagcttt tgaacaagtt agacaaatgg aagagcggat
    11221 gcgcgagcta gagattttca ttagcgaagg tacgccagac acacctgctt atagttctta
    11281 tttaaaagaa tacgaccggc tgcaacacga tttttcagat cgaaacggct atggttatga
    11341 aaatgaaatt cgttccgtat tgcatgggtt taaattcgat tcttcatttt atgatcagaa
    11401 aatcgatact ttatcaggcg gacagcgcac acgtttagct ttagctcgca tgcttttgca
    11461 acaccctgac attttgattc tagacgaacc aacgaaccat ttagatatcg agacactttc
    11521 ctggcttgag aattatctgc aaagctatac tggtgcatta ttgattgttt cccatgaccg
    11581 ttatttttta gataaagtag tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca
    11641 ttacaaaggt aactattcga aatatctaga tttgaaagca gaacagttag caagtgaatg
    11701 gaaggcttat gaaaagcagc aaacagaaat ccataaatta gaagacttcg ttgctcggaa
    11761 ccttgttcgc gcctctacca caaaacgagc gcaaagccgc agaaagacat tagaaaaaat
    11821 ggaccggctg gatcgtcctc aaggggatga aaaacaagca aactttctat tcagtataga
    11881 aaagccttct ggaaatgtag tcttgcaaat cgaagatgcc gctatcggat atgataatgt
    11941 caccttatct gaaccaatca atctagatat ccgtcgtcaa gaagcgattg ctctagtagg
    12001 tccgaatggt atcgggaaat cgacgttgct gaaatcaatc attcaccaga tcccttttat
    12061 ccgtggaaaa gaaacgctag ggacaaatgt caccatcggc tattacgatc aaggtcaagc
    12121 tgatctgcat acaaacaaga cgatcctagc agaattgtgg gatgagcatc ctaccactcc
    12181 agaaaaagag atccgaagtg ctttaggtgc tttcttattt tctggcgaag atgtagaaaa
    12241 accaatccct ctgctaagtg gcggtgaaaa agcacgggtt gctttagcca agttagcgat
    12301 gaacaaagaa aattttttga ttttggatga accaacaaac catttagata ttgacaacaa
    12361 agaagtatta gaaaatgcat tgatcgatta tgacggcaca ctgctttttg tttcccatga
    12421 ccgatatttc atcaatcgga tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa
    12481 agtctatcta ggagactatg attattattt agagaaaaag caggaagaag aagaaatcgc
    12541 cgaattgttg gcacaagatg agccagaatc tgctcctgct cccacgaaca aatctgatta
    12601 ttaccaaagc aaagaacagc aaaagcttat ccgtagcctt caacgaaaaa ttactcaggt
    12661 tgaagaagaa atggcgcgga tcgatgaatg gatcgatcag ttggaacaag aaatggtcgc
    12721 gccagaaaac ctggaagatc acgtaaaact caatgagctg aaccaagatt tagaagctgc
    12781 tagacaagat caagaaacaa aattgacgga atgggaagaa ctaagcttgg aattagaaga
    12841 atatgaaaat taacgaaaaa accaaaacag aaaccattca tgctgttttg gtttttcttt
    12901 ttttatttta tcaaacaaaa attccatatt tacctttttc accaataaga agaccaatta
    12961 ctagtcattc tcactaaagc ttctaaataa tagtaatcac cccaaataca aggtgtattt
    13021 accccattat ttttgggctt attataaaca gactgtgtta aaaagccatc ttcttcttcc
    13081 gttgtagcat atctttcttt caattgtcgc ataattttcc aaacagcaga ctggtataag
    13141 ttcttttctg ttttgtctaa tagacctgat tctattaatt ctaataatcc acaaaccatg
    13201 atcaccatac tagatgtatc tttttcttct ctgctagaat catcaaagat caaatcccaa
    13261 taacaaatgg tatcttctgg caaccgattc ataaaataat tggctaatat tttgcttaaa
    13321 tcaaaatatt tttgttcttt caaataacga taacttaatg caaaaccgta aatcccccat
    13381 gcttggcctc tagcccaaca ggaattatca ctataacctt gatgcgtact tccatatctt
    13441 ggttttccat ctttgacatc catataaaat gtatgataag ttgaaccatc ttctcttact
    13501 aaataatctg ctgcattttg aatatgagcc tgagcagctt ttttatattt ttgatttcca
    13561 gtttcttcac tcgcccaata caataatgga agattcatgc aacaatccat gatcattcta
    13621 ccttgttgtg aaggatcact aggatctccc caagcttgta taactcctgc cttctcccaa
    13681 aaacgctgaa gtaatgtttc tgcagcatca attgaagctt ccttcatttt tcgatttccc
    13741 gttattttat aatctgctac ggttgataaa gaatacaaaa aaccaagatc gtgatgatta
    13801 gtgataattt gacagtcaat cctgtggata aaatctatat ctgctttttc agctgtttgt
    13861 tgaaataaag gatcacccgt cttctcaaat aacaaccaat taattcctgt ccaaaatcct
    13921 tctgtccaac caatattgtc tcttaatgga taacgtcctt ttataggaca ctcgcttggg
    13981 aattgatact gactgttact gatacgtttg attaacctct ccgtgtgtct gcgacaacgc
    14041 tcgatctcat tattccattc tttttccatt aattctttat aacattcatt tatccatttt
    14101 tctggatgta ttatctcttc aaacatcact ttatcaagca tttccccacc agcctttagc
    14161 ataattttag tcttttttta ttgtttttat tttggatagt tagcccggtt gtcccatcaa
    14221 aatcaatttt gggacgattg ttccatactt ctaaactata atcccgatct tgatgagcaa
    14281 ccactcctgt tccgagccaa tgggttcctt ttccaaatgt accctctaat ccaggaataa
    14341 aagtgttttc ccatgtcatc aagtttgtat tgggcaaacc cataatagct gtggattttc
    14401 gtttaatagt atcgtcactt aaacagataa tacctgaaaa gccagcttca ctcaccctat
    14461 agttttcctt gtcttcttct atgattattc cttctttctc aggagaacat tcaatagcaa
    14521 accctgtctc atatagaaca tattctgtat ctaattctac tttgtgtacg cgaatctgcc
    14581 agccttcaaa aggaataagc caagttctga tttttgcctg taaagtaggt tcccatgtag
    14641 aggcaagtac ctctttctta catacaatgt ctcgtacatt atttcgaacg atgaactgtc
    14701 cataggtttt gatgctctca ttcttaaaac tggtcgtgat atatgtgtcc gctcgctgaa
    14761 tccctagcat tgaatccatt gccatttctg ctttcaattg attcgctcgc ggtatcgaaa
    14821 aaccaaattg actagaataa gcaaatttta aatatttttc attggtcaat gtatggaaat
    14881 ttggccctaa ttgtcccgca ttcaaaaaaa atgtatgtcc attatcatgt tgtatgagca
    14941 aattggcttc tgaaagaagt ttagttcctt cacgaatagg cataggcatt tctttagccg
    15001 tccaatactc atggtcttca ggtagcgcca acaataaaaa aatcttattc agccataatg
    15061 gagataacgt actattatag ggttcagtca atgtcaattg agggtaggta taaccaatat
    15121 tcaggatacc tttttcatca aatatttctt tttgcatcca ccatcttata ttccgattga
    15181 ctaacccctt gatttcacct aatttaaaag gcaaaagatc ggcccacaac atagctgacc
    15241 aaaagcttga aactgcaaaa cgataaatca tgcttcgacc aaaaggcaca tttgcaccat
    15301 cttccgcaaa aaaatgaata tagtctctag aaaaatcaat agctcggttc ttatatatct
    15361 ggctaatggg cttttctggt tctagtacgc tataaatcaa accataataa tgaaatgcaa
    15421 aaggattata ataatcttgg cgacctcttg aactgtctcg ataccagcca ttttgaagat
    15481 aacaattttc tatattatca aatgcttctt tcagtgctga ttctttaact ggataaccaa
    15541 atttttggat aattgttccg acaattatct taaaaaattg ccaattacca tctgcacatt
    15601 catattcaaa aatttgcgag aaccaatgaa taatctggct tttttctcct gtatctaatt
    15661 ttttccataa taattgttca tgatgatata acgctaataa cattgcgggc atttctacca
    15721 tacgctggtc tctttcatgt atttctcccc aataatcggg atgggaagga tctgttccag
    15781 cttttattcc tgatagaatt aatttcaacc atcgttcatc taattggtca attgcgggcc
    15841 ccgctcccca taaaacccga gagaatcctt ccattcctgc aattttattt ccatatccta
    15901 ccccttgatg tatataatca atttgtgcgc cacttgctcg ataaaacggc tggagtggtt
    15961 gatttatctt tttaaataag tcaaagtaat cctgtctggt ccttaatagt ggtactggat
    16021 gtttcattgt attttctcct ctttttacta ccaaatagct aattccgact gttctaatcg
    16081 aagtaagcct tcaataaaaa aataatcaga atagatcagt ggcacattat aagtcagttc
    16141 tctatggtat gcttctgctc cgcctccaat gatcccatct tcatccatat tccaatttgc
    16201 atagtgcttt tctgtctttt ggagtatatt gatagcagct tcatgataca tttttgatcg
    16261 tacagaggaa acatatcctg ctaattccag aagaccgcat gcagcgcata ctccagcact
    16321 ggtatcaata ttcgaatctt ttacagcaaa atcaatgaga ggaatataac cagttttttc
    16381 gattttctga ataaaattta ctgctatctt ttctgctata gctagatagt acggattttt
    16441 tgtgtaacga taacttaacg cgaatccata taaagcccat gcatttcctc tgctccaaca
    16501 agaattctca ttgtatcctt gcccgccaag taaccgaata tatttccctg attctgcatc
    16561 aaattctgcg atatgcccgg ttgatccatc tttcctcact agatacttag ctgttgtatc
    16621 tgcatgctgc cgagcaattt tagagaacct cggatcacca gtttccctcg aagcccaata
    16681 cagtaatggt aaattcatca tactatcaat aatcatccaa ccaggtttat ggtcattcca
    16741 tgctactaaa tagttccctg aaatattata gcgtccagca agtaccgtag ctgcatgtaa
    16801 ccctagttgt cgactatgtt tgttttgaga ctgttgataa tgtgcaatag ccgatggtaa
    16861 gaataaaaaa cctacatcat gatgaacgtc tgaaaaatgg tacagggcag attctaataa
    16921 ttgttcttgt tgtattgctg cctctttgaa cttttctcgt tttgtcttgt gaaagagctg
    16981 ccataaaata ccactccaaa aaccattggt ccaccagtcg attcctctgt cctttattac
    17041 gtctgtatat tttccgttgt aagaaatata tggaatttta ttccccattc tgtctaattc
    17101 cgcatctatc ttttcagaag ctacattcaa aatttttctt gaccattcct tcgtctgttc
    17161 attcatggat atcccgccat tcttttaatt tttatgaatg tggagttgct cctagaacga
    17221 actagatggt taacattcgt tatttttttt acttatttgc agctccaatg tcattctcct
    17281 taaatttaga ctgcgcccac acataaatca agaccattgg gatcactgaa gttgctaaaa
    17341 ttagcataat tgcgccataa ttaattggct cacctgtact agaaaccgtc atatttttga
    17401 taattaaggg aaccgtatat ttatctttat ctgaaagcat gactaatgga aatagatatt
    17461 gattccaact tcccataaag ataagtaaac cactagtaat aatcgtaggt gataaaatcg
    17521 gtacagcgat tttcgtaaaa atcacccact cattggcacc atcgattctg gcagcctcca
    17581 tcagtgctgt aggaaattga ctgctatatt gtcgcatcat aaacactgaa gttgtactgg
    17641 caatcgaagg gatgatcaca cctgcataag tattcgataa tcctaaacga ctcataatgt
    17701 aaaataaagg aatcaatgtg gtaaaaaaag gcaccatcct agatagcata actgtattga
    17761 acaacagttt attccccata aaacgatact ttgctaatgc ataacccgcc attgtagaac
    17821 tggctgtagc tgaaactgta ctgattacag caataacaat cgaattccaa ataatcaagg
    17881 gataattata tctcgccatg atggcttgcc agttttttaa tagcatatcc ccaaacttga
    17941 aattgattcc gccacttaaa atttcttgat tactttgcgt tgaagctaca atcatatata
    18001 aatatggcat caaaaatatt aaggcaccaa acacaataat gacgtacgta aaaattctgc
    18061 tttgtatctt ttgtttagtc atttttatca cctccgaatt taaattgaat agccgataat
    18121 ataacagata caaacaccat cgtccaggca atagcagagg cttttccaaa atcaaccatt
    18181 tcaaaacttg ttttatataa attgactcct acgaccaaag ctgaattttc tggtccattg
    18241 gaagtcccaa agaatatatt tgggatattg aaattaccca agccattgat agtggctaaa
    18301 actgtggcca ttaaaatgat tggctttagc atggggatcg ttaataaaaa gaattgccga
    18361 aatttcgatg ctccatctat ttcagcactt tcgtatatct ctttagaaat cccttgaaga
    18421 ccagctaaaa ataaaatagt gttatatcct acactaagcc aaatcatcgt gatgatcatt
    18481 gatattcgta tccaaaaagg atttgttaac cactgtacac cgttaattcc tagcgcattc
    18541 aaaaatccat tgaataaccc agtcggattg aaaaaagtca tgaatacgga agctacagcc
    18601 accgttgatg taatggttgg aatataatag acagttctaa aaaatccttt tccttttaac
    18661 tgtgctgaat tcaataaaac agcaaagtaa atagctaata gcatcgttat tggaattgat
    18721 cctgccatga taatcaaaac attcgtgtaa gatttaataa acaatggatc tcggaagata
    18781 gctagataat tgtcaaatcc aacaaattca aatgagtagc caattttcat aaaactaaaa
    18841 gcaaacgaga taatgatggg aatcaaaatc gtcacaataa agaaaataac aaaaggtgtg
    18901 accaatccat aagcactttt tttatgagac gtatctgttg cttttttctt gctgttcttt
    18961 attgtcttgc ttggtaacga agattgcatc atcgttaagt cctcctaata ttttttaatc
    19021 accagttaat tttacgccgt acttatgagc aaaattttct gcttctttta gatatgaatt
    19081 ttcaaaatca cctgtttgcc aatagttata tgcagcggta gtcaaataag acgtagcatc
    19141 tgaataagtg gctggtaaaa cagtatttga tgtattttca ttgattttat tcaatagttc
    19201 atagtatttt tgtcctcctt agtagtcaaa ctttttctca gctgcttctg tttgataggc
    19261 caatgagtta gatgtagcca cgcccagctc cagtgcgttg gctaagcatt cttcatctgt
    19321 cagcataaat gtaagaaatt gttgtgccac ttgtggattt ttggcatctt tagggacata
    19381 aaaacttgat cctcctgata ctgcaccttt tcctggattt tcttttgaaa aaggaatcag
    19441 tggagccatg cgccatttgt cagatgcatc aggaaaattc aaagacatat tcgtagcata
    19501 ccagccgcca gcaacaaaca ttgcagattc ttgaaatgct ttctcagcat cttgtgcacc
    19561 gtagaaatgt acaatatctg agtcgatcat ttctttgatt aactttgcag catgtttcgc
    19621 ttctttagta ttcagacgga tatttccttc ttcatcaagc aaagattgat tctgctgggc
    19681 tagcatgttg ataaacaatt cttggtcccc agattctggc aaggcaattc caaaactacc
    19741 agttttttct tttaatgctt ttgccacctg gatatattct tcccaagaac tgatttcttc
    19801 aaaatctaca cctatttcag caaatttttt tggttgataa taacaaagtg ctggggtaaa
    19861 atcactagcc caaggaataa tcgtttcatt actcgtttgt tgacgcaaaa tattcaattt
    19921 ttgatcataa aactgatcgc catatttgtc ataaaatcca tattcatcag cactataaaa
    19981 agcatcttga aatttttcca gcacacctgc tgctgaagtg tctgccatgg agataatatc
    20041 cggcatatct tcgtgtgtta ccaattttgg ggtgatcatt tgtactgttg ctgttcccga
    20101 agctacattc acaattttta agtcaatatt tgctccagtt ttgcggttat agatttttgc
    20161 agcattttca acatagtttg tcagtttgcc gtttgcgaca tatacttcta atgtagtggc
    20221 tttttttatc attggatctg cgatgtttgc tgttacttct tcgttagttt ttccacatgc
    20281 acttaagctg aataataggg taatgtttag caaagctatc accaatttgt atcttttttt
    20341 catgtctatc ccttctttct tttcaagttc ttatataaaa cgctttcact tatgtatatt
    20401 aacggaatta gtaacacaaa tatttatata aaaaaatatt ttacttgtcg ttttggcttt
    20461 tcatttttgt tataatagtc attaattata aatagaaagc tttttatata aaaagagaaa
    20521 ggaaagtggt tcttttgtta acaaacaacc aatcctccaa tcttgtcaaa aaaacactaa
    20581 cctattccat agatacccat cttcatttat ctgctgctcc ctattttcga ctactaataa
    20641 atgaaagcgc taatttatat gctgaatttg cacataaatc tatagaacta gataatcctt
    20701 cccttttatt cattgataaa cagcaagaag tccaaatctt ttctactcaa aaggaaacac
    20761 aaacttttat cattatccag ttttattttg atgagcaatt attagcacat attacgggac
    20821 aatctttttc tatagatcaa gaaataacat ctatcattac ttttctgaaa aatgaacagt
    20881 tcaaaattga ttatctaaaa catagtaagc cagctatttc ctatgatcag gaaatcaatt
    20941 cttttttcat actatgccaa cagatgagca ttcatttgct ttccttatta gttacttatt
    21001 gtctgcaaaa aattcaattg tcttccttac caaggcatta tcaacaggaa cttgctttat
    21061 tgacaatacc ttcaaatgag aaagtcattc ctaaaaaaaa gcatattctt tattcagatg
    21121 aattagttga tccaatcata cgatatttag aagaacatct ttcagaaaat tttttgcttg
    21181 aacaattagc ccaaattttt tttatcagtc cagccagctt aaaaaaacat tttagaaaag
    21241 tgaatggaaa aagtgttatt agctatttta aagaactaaa aatggaaaaa gcaaaacgtc
    21301 tcgtgctaga aaatgaatta acctatacag aaattgcaga tatattagga tttcattcga
    21361 tccatc
//
LOCUS       DAXLPI010000045        20861 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXLPI010000045 DAXLPI010000000
VERSION     DAXLPI010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.045"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(565..1590)
                     /locus_tag="ACF3T5_001405"
     CDS             complement(565..1590)
                     /locus_tag="ACF3T5_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3632018.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1865..3550
                     /locus_tag="ACF3T5_001406"
     CDS             1865..3550
                     /locus_tag="ACF3T5_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFD3632019.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3564..4847
                     /locus_tag="ACF3T5_001407"
     CDS             3564..4847
                     /locus_tag="ACF3T5_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3632020.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4917..5786
                     /locus_tag="ACF3T5_001408"
     CDS             4917..5786
                     /locus_tag="ACF3T5_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632021.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5783..6619
                     /locus_tag="ACF3T5_001409"
     CDS             5783..6619
                     /locus_tag="ACF3T5_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632022.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KVRSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6645..7712
                     /locus_tag="ACF3T5_001410"
     CDS             6645..7712
                     /locus_tag="ACF3T5_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFD3632023.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7709..8536
                     /locus_tag="ACF3T5_001411"
     CDS             7709..8536
                     /locus_tag="ACF3T5_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFD3632024.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8581..9606
                     /locus_tag="ACF3T5_001412"
     CDS             8581..9606
                     /locus_tag="ACF3T5_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFD3632025.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9603..11786
                     /locus_tag="ACF3T5_001413"
     CDS             9603..11786
                     /locus_tag="ACF3T5_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFD3632026.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11786..12916
                     /locus_tag="ACF3T5_001414"
     CDS             11786..12916
                     /locus_tag="ACF3T5_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFD3632027.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12913..13590
                     /gene="pgmB"
                     /locus_tag="ACF3T5_001415"
     CDS             12913..13590
                     /gene="pgmB"
                     /locus_tag="ACF3T5_001415"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFD3632028.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13674..14201
                     /gene="msrA"
                     /locus_tag="ACF3T5_001416"
     CDS             13674..14201
                     /gene="msrA"
                     /locus_tag="ACF3T5_001416"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFD3632029.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14402..15088)
                     /locus_tag="ACF3T5_001417"
     CDS             complement(14402..15088)
                     /locus_tag="ACF3T5_001417"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFD3632030.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15168..16706
                     /locus_tag="ACF3T5_001418"
     CDS             15168..16706
                     /locus_tag="ACF3T5_001418"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFD3632031.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16826..17293
                     /locus_tag="ACF3T5_001419"
     CDS             16826..17293
                     /locus_tag="ACF3T5_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFD3632032.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17827..18255
                     /gene="rplM"
                     /locus_tag="ACF3T5_001420"
     CDS             17827..18255
                     /gene="rplM"
                     /locus_tag="ACF3T5_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFD3632033.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18271..18663
                     /gene="rpsI"
                     /locus_tag="ACF3T5_001421"
     CDS             18271..18663
                     /gene="rpsI"
                     /locus_tag="ACF3T5_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFD3632034.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18751..19977)
                     /locus_tag="ACF3T5_001422"
     CDS             complement(18751..19977)
                     /locus_tag="ACF3T5_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3632035.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20049..20351)
                     /locus_tag="ACF3T5_001423"
     CDS             complement(20049..20351)
                     /locus_tag="ACF3T5_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632036.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
BASE COUNT         6738 a         3743 c         4329 g         6051 t
ORIGIN      
        1 ttgtcacaac ctcttttttg gtttgtctaa aataatttgt ctaactagaa gaaaagtggg
       61 aaaggatctt aacaacatcg gttcgtatcc aattaaggta tcggaacgat catcttccaa
      121 gagaagtcaa tcaacaaaag taaagataga tcattttttg aatgcaaaat aaattgatgg
      181 gaagatagag cggttatttt taatattttg ctagctatta ttgaataatt cctcgaataa
      241 aacaaaacca gtagacatgt ctgttattct atttatgttt tagagataag tatccccaaa
      301 taacatggat tgaaataagg gtaaatgtgt ttaaaaaaac gaactataaa aacttataca
      361 caacgtggtt aaaaatagtt ttttcttccc tgtggaaaaa atagtttaac acaaacatta
      421 tagttatcca cagctatcta aaacagatgg aaaataagaa aaattcgcta aaataccggg
      481 agggggtaac gagggatgag tgattgaaaa aagaacagag taactgactt atcctcagtg
      541 tttctgttct tttataattc tgttctacat ttcttgttgt tggatcgaat ctgtgagagt
      601 aatctcggtt gaaagaatga ttcttttttc ttcagggtat tggtggtgga ttagttggaa
      661 taaaagttct gtggctttga ttcctaactg atttgtcgga atgtctatcc gagtcaactg
      721 tggcgaaaat agggaggaca ataagggatc actgaatcct gcgatcgaaa gatcatcagg
      781 tatctgcttt ccagattgtt ctgccacttt gtaaatactc aatgatttta atgcatcggt
      841 agaaaaaatc gcagttggtc tttctcgtag cttcattaaa tcattcattg ctgccatgct
      901 atcttctaag cgaaagccgc cgtctataat ccattcatta cggatatcta ggtggtgatc
      961 aaataagcat tgacggtaac cattgagacg atcattggat acataaactt ctgtcggagc
     1021 atggagacag ccgatatgac ggtgtccatt cttgatcagt gtttccacca aatcgtaact
     1081 atcctggaaa ttatccgtgt ccactgagta gatgttcgtg tagtcgtttc cgacttttcc
     1141 gattaccaca ataggaatat tgtattgatc caattcaaaa agaagtgttt catccatcgt
     1201 agagcttaaa ataataattc ctcggatcat tttttcttgg atctttttcg cgcatttttc
     1261 tagctcttct tctggtgttt ttgccgtttg tagaatcaag tcgaagttat tttgttcaga
     1321 aacggcagaa atggattgaa taacatctga aaaaaaagga ttgccatagg tcgtatgaga
     1381 agtacgatga gataatgcta gaatcgcgtc aaaaccattt cctgacagtc ctcttgcaat
     1441 tttgctcggt tcataattca attctttgat tgcttgaagt acacttgctt ttgctttatc
     1501 agagatattt ggctggttat ttaatactct ggagacagtt gattttgaga cattagcttt
     1561 tttcgcaatg tcataaatag taggtttcaa ttttatatgc acctcctttt tgggagcgtt
     1621 ttagaataat tccatagtac aatagacatt cttttttgtc tatgattttg aaaatttagg
     1681 aatagcttgc atgatcatga gagaccatat ttttttaaga agctattaat tattgataca
     1741 taagctttct tatttgtatt tttgtgtata tcaaaattat ttaaaaaaat gctattgaca
     1801 gtaagcgttt tccataatat gatcaaatcg ttgggaacgc tcccaaaaaa gaaaggggaa
     1861 tccgatgtat acaaaacttt tacaagcgat ttatcgcgac tcagatttgt ctgagaaaac
     1921 cgcctctatg attgaacaaa aagtcagtgt ttatcagcag aagaaaggcc aggcaggcaa
     1981 actgcattgg gacgaagaag atattttctt gattacttat ggggatcaat tttttgaaga
     2041 gcagcaacca actttgcgta catttaagaa attttatcaa aagtatttgg cagatacatt
     2101 tagagttgta catttcttgc catttttccc atatacatct gatgatggtt tctccgttgt
     2161 aaattatcag gcaatcaacg accgtttggg cacatgggaa gatatagaag aaatgagaag
     2221 cgaaacacgg ttaatgttcg actttgtctg caatcatatg tctgccgaaa gtacttggtt
     2281 tcaggaatat ttaaacttga atgatacgta caaagatttt tttattgatt tgccactgga
     2341 tacagataca agcagcgtga ctagaccaag gaccagcccg ttacttacaa cgttcattga
     2401 caaaaacggg aaagaaaaac acgtatggac gacttttagc gaagatcaag tagacttgaa
     2461 ttttaaaaat ccagaagttt tattgaaaat gatcgatatt cttttgtttt acttagaaaa
     2521 aggcgcttca tttattcgct tggacgctgt gggattttta tggaaagaac taggaacttc
     2581 ttgtatacac ctgccagaaa cgcataaaat catccaatta ttccgtgcaa tcgctgatga
     2641 agcagctcca ggaacggtat tgatcactga gacaaatgtg ccacacaaag ataatatcag
     2701 ttattttggg aatggcgaga atgaagcaca gatggtctat cagttttcgc taccaccttt
     2761 gacccttcaa gcgattcgtt cgggtaatac aagtaagttg aaagaatggt taaaaaccat
     2821 cgatttcgca tcgaaaaaaa caactttttt taatttttta gcctcccatg acggtatcgg
     2881 attgaatccg gtacgaggta ttctgccgga aaaaacgatc cttgaattag tggaacagct
     2941 gaagaaagag ggtgcccgag taaactacaa gaaaaaccca gatggaacga ccagtccata
     3001 tgagataaac gctacgtatc tagatgcgtt aagttctcca acagatacga cggaatggag
     3061 aagcaaacgc tttttagctg ctcattcatt tttggtaacg ttaccaggcg tgccggcaat
     3121 ttatattcaa agtgtgctgg gaagtcgaaa tgattacaaa ggcgtagaaa aaacaggaga
     3181 gaatcgaaaa atcaatcggc aaaaattttt gttagcagat attgagaaag agttgcagac
     3241 agatccatta agaacagcag tatatggtga attgacccag atgctatcta ttcgaaaaaa
     3301 agaaaaagcc ttccatccga atagtttgat ttacttactg gactcagcag attcttttat
     3361 cagttttatt cgcgaagcga aggaagagaa agtcttagtt atccataatt tttccaatca
     3421 atcagcaggt tatcttttac caaaaggaga ttacctagac ctgttttctg gacaaattat
     3481 ccattgcagt caagaacgaa agttgatatt ccagccgtat gaatatatgt ggctgaaaat
     3541 aataaaataa gaggtggaaa aaaatgaaaa aaattattgt tactacctgt ctgctagcta
     3601 ctggatttct tcttactgct tgtggtgaaa gtcaaagctc taacgaatca caacagacaa
     3661 cgattcaatt catgcactct tctgtcgaac aggaacgatt aagtgtgatc aatgaactgg
     3721 ttgctgattt tgaaaaagaa aatccagaca tcaaaatcaa gcaagtgccg gtagaagaat
     3781 cagcgttcaa tacaaaaatc gttacgttag ctcgttccgg taaattacca gaagtaatgg
     3841 aagtcagtca ggattttgct aaagtgatgg ataaagatga actgattgat cagacggcag
     3901 tccaaaacgt tatcgaggaa gctggagaag ataattatta tgacggagcc aaaaaccttg
     3961 ttcgttcaga agatggaaaa agctatattg ctgcaccaat tagcggctgg gttcaaggta
     4021 tttggtatga caaagaaaaa ttatcagaag ctggtttttc agaaccagaa aattgggatg
     4081 atattttaaa aattgctcaa catttcacag ataaagagaa taaacaatat ggaattgcta
     4141 tgcctactgc tgaaggaaca atgagtgagc aagcgttctc tcaatttgct ttgtcaaatg
     4201 gagcaaatgt tttggatgat gaaggcgagg taacgatcga tacggaaaag atgagagaag
     4261 cattgtcgtt ttatcaaaac ttatcccaat acacgatgcc aggctctaat gatgtgacag
     4321 aaatcaaaga tgctttcatg aatggaacag caccaatggc aatctattca acttacattt
     4381 tgccttctgt ttatgaagcg gggaactcag aaaatatcgg ttttgctatc ccaacacaaa
     4441 aagatcaagc tgtctatggc acagtatctg gattgacgat ttcttctggt ctagacgaga
     4501 aacaaaaaga ggctgctgaa gcattcacag ctttcttatc tcaaccgaaa aacatggaaa
     4561 agtgggtctt gatgtctcct ggtggtgctc aaccagtgaa taaacaagta gtcgaactgg
     4621 atggttacaa agaaaatgaa gtcatcaagt cttttggcga gctgccatct gagattgcta
     4681 gtgcttttga tgaagtacaa gtctttggac tagtcgggga aaagaatttc acgaaaatgg
     4741 gagatatcac aagctcaggt gtgatcggaa aagcagtgaa ccaagtaact gtcggaaacg
     4801 aagatgtcga tcaagcttta gctgacgcac aaaaaaatac gaaataacaa gaagttctgc
     4861 caatggtgtt cgaaaaaagt agatagctat tggcagatta tcagggggag gtttcaatga
     4921 atcgtccaag gaaacattct gacttaaaat tagccatggg acttttagca ccaagcatgc
     4981 ttttattagc tgttttagtg ctgattccaa tgctttccaa tattcagatc agttttttcg
     5041 atatgccgct gaatcctaac tcagacgccg tttttatcgg attaagcaat tatcttgaaa
     5101 ttttaagtga tagtgaattt tatcgttctt taggtttaac agttgcttac acattcttag
     5161 ttgtagcagg cagtacagct ttaggattag ccgtagctat tttgcttaac cgcaagttca
     5221 gatttaggaa aacggtgcgt tcactgattt tattaagtta tgttacgcca tctatttctt
     5281 tgatttttgc ttggaaatat atgttcaata atagttatgg aatcgtcaat gatctgttag
     5341 tagataggtt gcagcttttc aacgaagcac cgctgtggtt cgaccagcca gttgctagct
     5401 tctttatggt cgttttgttt gctatctgga gatactatcc gtatgcgttt atttcatttt
     5461 tggctatttt gcaaacagtc gacaaaacgc tttatgaagc agctgaaatg gacggtgcaa
     5521 atgcttggca gaagttcaag atcgtgacac tgccggcaat caaaccagta ctagcaactg
     5581 tgattgtttt acgatctatc tggatgttct atatgtttac agatgtctat ctactgacga
     5641 ataaagtcaa tatcttaggc gtttatttgt atcagacagc ctttgcgttc aatgatcttg
     5701 gaaaagcagc agctatatcg attctattgt tcatcattat tgcagcggtc gtcttgctct
     5761 cacgaaagaa ggtggacttg aaatgaaaaa taaaaagagc aggaaaatct tattttacgc
     5821 gacgattttg ttgctattag ctgtcaattt gtttccattt ttgatcatgc tgatgacatc
     5881 attcaaggat tcacaagaag caattgcaac gacacaaact atttttccga aagaatggac
     5941 ttttgaacat tatcaggata ttttcaatgc tgagattttt ccattcatca tgtatttcaa
     6001 aaatagttta gtgatctcct tgatagcctc cgtaactgca gtagtgattg ggatcagcgg
     6061 ggcatacgct ttgtcaaaac tacgcttcaa agttcgctcg aaaatcaatg ctagctttta
     6121 caccgtttat atgttttccg gtattttgat cgttgttcca ttatttaaaa tcatttccgg
     6181 tctaggtttg tatgatacca atacagcact gattatcacg atggtcgttc aaacacttcc
     6241 tactgcgata ttcatgctaa aaagttattt tgacacgatt ccgactgata ttgaagaagc
     6301 agcaatgatc gatggtctta gccggatcca gatcattatt cggatcatta taccattggc
     6361 tatttctgga atcatttctg tcttcgttta ttgctttatg gtttcgtgga atgattattt
     6421 atttgcttcg attttcctgt cgacctccga aaaatttact ttaccaatcg gtttgaacac
     6481 attattcagt acaccagact acatttgggg acgaatgatg gccgcttcat tggtgaccgc
     6541 tttaccagtc atcattatgt atgcgttatc tgaacgattt attaaaggtg atttagtcga
     6601 aggcggcgta aaaggataaa aagaaaagaa agaggaaaga aaagatgaaa aaattagttg
     6661 cattagaacc aagaatagcc ggattcaaag attacgaaga ccgtgagatc gaaaagaacg
     6721 aagtacgtat ccgagtaatt ttcgctactc caaaaccagg aacagaaatt gtcgacttta
     6781 gaggattaag tccatttatc gatgaagaat tttcagaaga atggcgccta ttcttgccaa
     6841 gagaagaagg aagccaacgc ggtgtggtat ttggcgaatt tcaattagga aatatgattg
     6901 tcggtacgat cgaagaaacg ggcagtttgg tgacagagta tcaaaaagga gagttagtct
     6961 gcacttatgg acccattcgt gaaacgctga ttacaaacgg tgttgataat tataaactac
     7021 gtaaacttga tcggttggat caatggaaaa atgcagtatg ttacgatcct gctcaattcg
     7081 ctttaggtgg tatccgggaa gcacatgttc gagcaggtga tgctgtagca atcttcggtt
     7141 taggcgctat cgggcaaatt gcgattcaat tagctaaaaa agcaggtgct actaccatcg
     7201 ttgggatcga tcccatagcg cagcgtagag aattagcaga aaaatacggt gccgattaca
     7261 cgctagatcc tatgaaagca gatgtcggtt ttcgtctaaa ggaattgaca gataaacgtg
     7321 gagtagattc agttattgaa acgagtggca gtgctgccgc attacaagct tctttaagag
     7381 ggatcggcta tggcgggatc atttcttatg ttgcctttgc taaacctttt ccagctgggt
     7441 ttaatcttgg tcgcgaagca catttcaata atgcaaaaat cgtctttgct cgagcatcta
     7501 gtgaaccaaa cccagattac ccaagatgga accgtaaacg aatcgaggac acatgttggg
     7561 aattattgat gaacggttac ttggattgtg aagaaatcat tgatccagtt gtgccatttg
     7621 cagaaagcgc agaagcttac atgcgttatg tagaccagca cccagaagaa agtatcaaaa
     7681 tgggcgttca agtccgagga ggagaacaat gaaattcggt acgcaagatc aagcattttt
     7741 cccaagagac attttagaaa aatttagatt tatcaaagac tgtggatttg aagaatacga
     7801 aatcgacggt aagttgttaa tagaaaatat cgacacagtc aaacaggcaa tcaaagaaac
     7861 agggatccca gtagtgacag cttgtaacgg ctacgatggg tggatcggtg actttatcga
     7921 agaacggcgt cttaatggtg tacgtcaaat tgctgagatt ttgcgtgcac taagcgaggt
     7981 tggaggcaaa ggaatcgtta tcccagcagc ttggggaatg ttcacttatc gattaccgcc
     8041 gatggtttca ccccgaagca aagaaggaga ctataaagca gtcagcgaat cacttcgtta
     8101 tttagataaa gtagccgaag aaacaggtac ggttatttat ttggagcctt tgaaccgata
     8161 ccaagaccat atgatcaatt tgttagcgga tgcgcgtcat tacattgact ctaatcaatt
     8221 gaaacatgtg cagatcatcg ctgattttta tcatatgaat attgaagaag atgatatttc
     8281 aaaagcattg actacgcaaa aagagtatgt gggtcatatc catttagcag ataatcatcg
     8341 ctaccaacca ggatctggcg caattgattt tactcgccat tttcaaacgc tacttcaaaa
     8401 tgagtatgaa ggttatatgg tatatgaatg tcgtttgcga ggagaaaatg aagtgaaaat
     8461 ttatcaggaa tcattgcaat acttgaaaaa ctgtctggaa acagcgcgtc atttaaatga
     8521 tttagagaaa gaatagagaa agaataacaa taagtaaata agcaaaggag ggagaaataa
     8581 atgaagaaag tagcaatcat aggcgctgga caagtagcag aaaaagtaca tgctgcttat
     8641 tatcaaaaaa gatcagatgt cttagaattg gccgctgtgg ttgatccagt cagccagcga
     8701 gcagaagact tttgccgacg tacaggattt tctcatgcgt atacatcagt tacacaaatg
     8761 ctgaatgaga tttctcccga tatcgtgagt gtctgtacac ccaatcgctt tcacttcgaa
     8821 catgtattgg ctgccctcga agctaatgct tcagtgatgt gcgagaaacc accagctatg
     8881 acagctgatc aagcaagaaa gatggcagag acagccgata aaaacgggca gattttggct
     8941 tatggattcc agcatcgttt ttctgacgaa gcaacaatga tcaagaatca tcttgatgtt
     9001 ctcggggata tttattacgt agaagcgaat gcgcttcgtc ggtctggtgt tccaggctgg
     9061 ggaacgttta tcgacaaaga acttcaagga ggcgggcctt tgatcgatta tggcattcac
     9121 atgcttgata cagcgatgta tattactgga tttcctaaag taaaaagcgt tcgggcgtac
     9181 agcttccaaa aaattggtac gaaaaaaaat tcagggaaat tcgggaaatg ggatccaagc
     9241 aaattcacag tagaagatgc aatttttggc acactggaat tagaaaacgg caagatcatc
     9301 caactgaata cttcgtttgc tttgaatatc agagaagaca aactgaatgt ggctttttgt
     9361 ggcgatcagg caggtgctaa tctgtatcca ggcgaactat tcgaagatgt agatggaaag
     9421 ctggtttcct tacaaaagac cacgccagtt aatgacccac atgaactaag tatggctcat
     9481 tttgtgaatc gggcgctcgg tgaccagact cagatgattg cagatggctg gcaaggatat
     9541 gtcatccaga atctggtgga aaatttatat cgttcggcag aaacaggaga agttattacc
     9601 ttatgaaagt agaagaaaaa cagtttgatt tagagtatat caataagtat gcgagtcaga
     9661 tgaccattgg aaatggattt tttggtatca gaggaagcca ggaggaagac tatcggaatc
     9721 aagtaagggg aatgtttgca gctggtgtat ataaccgacc tttaggagca gaatcagccg
     9781 agttagtgaa tctcccagat gtcatcagat gggaaataaa aatcgacggt gaacattttt
     9841 ctcttgagac gatgaaagtt cttgcatacg agcgggcact agatttagaa atgggagagc
     9901 ttaaacgcta tgcgctcatt gaaactgctc aaggaaaaaa agtggaagtc attacttgtc
     9961 gtgttgctga tcaaaataat ctccatcagg cagccctgaa agtggtcatc aagccgttga
    10021 ccgaagactg ccaagtagat gtgaaaacgg gtatcgatgg acagcaaacc aattttggta
    10081 cacagcagtt ggttgaaaaa gaactgcgag tatttggtga cgatttgatc agtgcatcct
    10141 accagacaac agaaagtaaa attgatattg ggattgcagc taaattcaat aaagcaggtg
    10201 tattccaagc aaaaaatcgc cggatcaccg cttccttttc agaaaagatc agaaaaaata
    10261 cgtcctttac gctggagaag atcatcgtgc tctctacctc tcttcagcaa acagacccgt
    10321 ttaagaaaag tattgcccaa gcccagaaaa gcgttagtta tacagaaatt ttagagcagt
    10381 cgatgtctta ttggacagat ttttggcatc agcatcggat aacgatccaa ggcgattctt
    10441 ctttcgatca gttagcgatg gacttcgctg cttaccattt acagattatg actccccgca
    10501 atgatccaag tgttagtgta ggggcgaagg gactaactgg cgaaggttat aaagggcatg
    10561 ttttctggga tacggaaatt tttatcctgc ctttcttttt atatcagttt cctgaagtag
    10621 caaagaattt gctgctgtac cgttatgatc gattagaagg cgcacggcaa aaagcaagag
    10681 attatcacta tcaaggtgca cttttcccat gggaaagcgc acacactggt caggaagaga
    10741 cacccaaatt tgctgcattg aatatcaaaa ccggaacaag acaagaagtt gcttcagctg
    10801 ccgcagaaca tcatatcagt gcagaccttg cttatgccgt tcaagagtat gtccaagcaa
    10861 cagaggatca atcgttttta gaaacaaagg gaaaaacgct cattcatgaa acagcagctt
    10921 tttggctgag cagagctgta aaagtcgatg atcatttaga gatacacaga gtcattggtc
    10981 cagatgaata cacagaatat atcgacaata atgcatatac gaactatctg gcgcattata
    11041 atgtggcctt agctgttaaa tatcaagctg gcgatccgct ttttttgaaa gaatgtcaaa
    11101 attttttaga taagttatat ttgccagtgc ctaacaaaga acagctgatt ccgcaagatg
    11161 acacgtatct cagtaagcca gttattgatt tgaccactta caaagaacgt cctggcagcc
    11221 agtctatttt acttgattat gcccgtagcg aagtaaatga actccaagtc agcaagcaag
    11281 cagatcttgt catgctgtta tacttattac ctgagtattt ttctcaacca atcgtttcta
    11341 agaatttgca ttactatgaa aacaaaacaa tccacgactc atcgttgagt aaagcgattc
    11401 acgccataga ggcttgccgt atccaagaaa tggaatgggc gtatcattta ttccaagaag
    11461 cgtgcctgat tgatttagga ccagatccgc attcatcaga tgacggaatt catgctgctt
    11521 cattaggagc actatggcta gcagttatat ttggtttttc aggtattaca gtaacgaatg
    11581 gaactctttc tatttctcca agacttcctt cagcatggga aaaaatttct tttcctttta
    11641 tttggaaaaa tcacaggttg ctatttgttt tgacaaaaaa acaaatagta ataaaaaaag
    11701 agacgaaaga aacattacct atttttgtca atggagacca ataccagctg gaaaaagaac
    11761 tgatcttgga acggagggga aaataatgcg tattctgatt gcgccagatt ctttcaaaga
    11821 aagtctgaca gccttagagg cagcacaagc cattcaatca ggttttcaag aagtttttgt
    11881 tcaggctgaa tatgatctgc tacctatagg cgatggagga gaaggaacgg tttcatcatt
    11941 agcagacagt ttaaggttga agcggcaaac agcagtcgtt actaatgcat ttggcaaaaa
    12001 caaagaaatc cagtacgctt cagacggtca aactgcgatt tttgaaatgg cagaaatcgt
    12061 cggattgaaa gatgtcccac taaaccaaca acagccactt aggttatcca cgcgaggagt
    12121 aggagaactg atacgacaat tgttgcaaca aggtgttcga cacatcatca tcggcgttgg
    12181 cggaagtagt accaacgatg gcggcatcgg catggcagaa ggtctaggtt atgcttttta
    12241 cgatcaagat aatcaaaaag tagaagcact gggagaaaat ctaggtaaaa ttgctacagt
    12301 tgataaaacg aacgtgccta ttgagctgat taaagccaaa atcactgtca ttactgatgt
    12361 tactaatctt ttatgcggaa aagaaggcgc ttcttttgtt ttcggaccac aaaaaggctt
    12421 gaaagaaaat gaattagcag tggctgacca agccatgcat gatttttata tgagatttgc
    12481 ccctcaagta atcgaggcat cgggcagtag tgctggtggt ggaatgggtg caggactgtt
    12541 agcatttgct tcagggaaaa tagtctctgg catcgactac gtattggatt tactggagtt
    12601 cgataaacgg gtacagcaag cagacctcgt aatcgtaggc gaaggacgtc tagatggaca
    12661 aagtctaaaa ggaaaagcgc cagttgggat tgctagaaga gtaccagagg gcgttcctgt
    12721 gatagctatt tgcggaagtg ttggtaaagg aagcgaaaaa gcagcagatg ttggcatttc
    12781 ggccgttttt ccgatccttc ctgcagctgt gcctttacaa gaagctttac agcaagccac
    12841 tgataatctt catcgcacag ctaaaaacat cgcatcttta cttcaagtcg ttcattttca
    12901 aggaggtaat cgatgaaaat caaagcagca ctattcgatt tagatggcgt tcttgtagac
    12961 acagctcgtt atcattacga agcgtggctg gttttagcta accagctgtc cattccattc
    13021 actgagaaag aaaacgagca actgaaagga atatccagaa ccgagtcact agagcgattg
    13081 ctgtcatttg gaaaaatgga acagaaattc tcagaaaaag agaaatcagc ctttgctgag
    13141 caaaaaaaca atctatatct tcaagcgatt caaaaaatgg atgaaacatc cgttttgcct
    13201 ggtgcaatag ctgttcttga gtacttgaaa aaaacaaaca tcaagatcgg attgggatca
    13261 gcaagcaaaa atgcacgcct cattttagaa aaaacgaatt tgacaagtta ttttgatgtc
    13321 cttattgacg gcacgcaagt atcaaaagca aaacctgatc cagaagtttt tctcaaagga
    13381 gcacagcaat tgaatgtacc gccaaatgca tgcttggtca ttgaagattc agaagctggt
    13441 tgtcaagcag ctttggctgg taacatgcat gtattgggaa taggtgaaaa cataaatctt
    13501 ccttctgctg aatatgtgat tcctgattta accgtttttg atcaagtacg ttcgttttgg
    13561 catttatcag aagccgtatc acgtatataa tagtttccgt tttttctgtc acaagcaaaa
    13621 cgttatcctc cttttggaaa tcaaggtatg atgagaaaaa agggggattt tatatgacaa
    13681 aaaaagcagt tttcgcaggc ggatgctttt ggtgtatggt caaaccattt gaagagcagc
    13741 cagggatcat ttctgtgaca tccggttata ctggcgggca tgtaccaaat ccaacttatg
    13801 aacaagtaac aacaggcaca acagggcata cagaagcagt agagatcgaa tatgatccag
    13861 aagttatttc ttatgatcag ttggtggaaa tctattggca gcaaaccgat ccgactgacg
    13921 cattaggaca gttcgctgat cgcggtgatt cttatcgtcc agtgatctac tataatgatg
    13981 aagaagaacg aaatattgca gagcgttcaa aacaagcatt gcaagacagc gggcgattcg
    14041 ataagccaat cgtgacaaaa atcgaaccaa gaagcgaatt ttatcctgca gaagattacc
    14101 atcaggatta ttacaagaaa aacaagctcc attacaatct ttatcgagaa ggttctggac
    14161 gtgcaggttt ccttcgtcga cattggggcg tcaagaaata agaagccgaa aaagctactt
    14221 atggaacacc gtatatcaga ggttgtaaaa ccagcatttt gagctaatac tgagggtcag
    14281 ctgattgaac accatttagt ggagaggttg tgaacctgtc gcctagctcc aagtcacaag
    14341 gatttggtaa gagaacacta tatatcagag gttgtgacaa gtcgtttgtc acaacctttt
    14401 ttcaatacct aagatgaagg acaatcagca acgcactgct caaaaaagga atgaaaggca
    14461 gcttttccaa ctttttttta tgacagataa agtaaacaga aaaaacggat attccaaaca
    14521 gactagcaag aaacaagatc tgaagcaact cttctagaga caagccacca ctccaagaaa
    14581 gcatcaacag agtatcgcca gctccgagat aggattgacc atagcgaagg cacacaccgc
    14641 ctacgataaa cgttaacagc acactttgcc attggaagac gccggcctgc aaccaaaaaa
    14701 tccataaaat tgcccaagaa cagaatagcg tgtgagaatg gaccatgaag taaaataaat
    14761 ccatcaacga aaatagcaaa gccgaaagca tccatatgaa cgtcgaaaaa tttcttgcgg
    14821 aagaaaaagt aaagaaccag gcaaaaagac caccacataa ggcttctgcc aacaaatagt
    14881 agaagggcaa ccgacacctg caatagcggc agcgaaatcg ttggacgatg atcgagagaa
    14941 tcggaatcaa ttcataccaa gaaagcgaac gacagcattt gacacaatga gatctaggat
    15001 aaataataga gtgacctaac ggaatccgct gggcggttaa acataaaaat gatccgatac
    15061 agcaaccaat gataaaataa agtaacacgc catctccccc ttttcaaaac caagatacgc
    15121 aagcttaaac taaatttttg tacatacata aaatttgagg tgaaaaaatg aacgtttcaa
    15181 tcggattaga tatcggaacc acacaaacca aagcagttgc tttcacagat gaagcccaag
    15241 aagcagcatc cgcttatttt cgctaccctt tgatccaaga aacacaaggg atggcagaac
    15301 aagatcagga attgatcttt caaggtgtct gcaccgttat aaaagaagta attaaacagt
    15361 tgccttcaga cacaatcatt caatttgtct ccttctcctc tgcgatgcat ggcctgatat
    15421 tgttggatga agagaaaaca ccgttatctc gaatgatcac ttgggcagat aaccgagcag
    15481 cggacgaagc agaaaaactg aaatataccg tgcttggcaa agaattttat cggcagactg
    15541 ggacgccgat tcatccaatg acaccattga acaaaatcaa gtggctgcaa aacagccaac
    15601 ctgaattatt caaaaaaact gcttattttg tcggtatcaa agactatatc ttttatagat
    15661 tgtttggcga actgatcagc gactacggaa cagcttcagg aactggatat tttgatatcc
    15721 atgaattcca atgggcagaa gaagtgcttg actacttgaa cattcgaata gagcaattgc
    15781 cgcaagtctt tccttccacc tatcaagtaa caggtctacc atcaaaaaca gcagcagaac
    15841 taggtttgcc aaatgatata tcatttgttt tgggaggggc agacgggccg ctttctaatc
    15901 tagggttagg tgcttttggt gatacgatcg ccgctttgac cgtaggaacg agcggagcac
    15961 ttcgttttat tactactcag cctcagttcc atccagaaat ggagacgttt tgttatgttc
    16021 tggatcagaa acattgggta gtgggaggag cgactagcaa tggtgctggc atctttgatt
    16081 gggcagggca tacattttta caggaagtcg ttcaatctgc taaagaaaaa ggagaaaatc
    16141 catatgatgc actgctttcg gctatcgctt ttgtgccagc gggtgccaat ggtctgattt
    16201 ttcatcccta tttacttggt gagcgagcac cgctttggga cgcagatgcg tttggcagtt
    16261 ttatcggttt acagcggcaa catacagaaa aagaaatgat gaaggcgatt ctagaaggga
    16321 tctgtctcaa cctttatcgg attttaaaag gagtttgcca ggaaaatcag ctcacagaga
    16381 tccgtgctac aggcgggttt gctcaatctc cgatttttcg tcagatgatg gctgatgtac
    16441 tgggttaccc gcttgttttc cctactgtca cagaagcatc cgcattagga gctgtgattt
    16501 taggctggag aagcatcgga aagatccaaa gtatcagtga agcacaggaa ttgatagaat
    16561 tgaaagagca agttggagta caagaaactg atcataaaaa atatcagaaa atctatccat
    16621 tatttgtttc gacacaaaag catttagcac agacttatca gcaatttgct caactgcgag
    16681 aagaattagc tgattctgaa aaatgattgg ctttacggat aaaaaagcga actgaaaatc
    16741 gttctcaggt aaatgatttt gttgtacttt tcattgaaag tctactatta ttttaagtgt
    16801 aagaaaatgt taggaggcaa gaaagatgaa attccaacaa accaaagaag tattaaatca
    16861 attagttgca gatctaagcc aaatgtctgt tgttgttcac caaacacatt ggtacatgag
    16921 aggaccaggt ttccttacat tgcacccaat gatggatgag ttcatggatg atttgaacgc
    16981 tcagttagat gaagtatccg aacgcttgat cacattagac ggttcacctt attctacatt
    17041 gcgtgaattt gcagataaca caaaaatccc tgatgaaatt ggccgctggg atcgtactat
    17101 tccagaacgt ttggaaacac ttgttgcagg ctatcgttat ctagcagatc tttaccaaaa
    17161 aggaatcgaa gtttctggag aagaaggcga cgatccaaca caagatatct ttatcgaatt
    17221 caaaacagcc acagaaaaac gtatctggat gatccaagct catttgggca aagcgccaga
    17281 gatcgacgcg taaaaactgt tgttgaactt gaccgaaaaa gaggttgtga aatcagcgtt
    17341 ttgacctgag tattagagaa acaaacgtca aaatagttcc tcatattttg ccgttttttg
    17401 agctaatacc gagggtcagc tgattgaaca ccgtttacca agaggttgta agcctgccgt
    17461 tcagctccga gtcacaagaa acaattttaa aaaacagctt cttatgtttt atggatattg
    17521 tgacttgtga ccgaggagtt ggcaggagaa caccgtttat tcaaaataag gggttgtgac
    17581 aagacttttg tcacagcctc tttttgtttt tcattttgac aggaaacgac ccttttatat
    17641 aaaagaaaga aaagaagtaa agtttcatat aaatttttca atcaagcaga aaaacgttga
    17701 cattgactag attagcgggt aatatgttaa atggtattgt ttgccccaca aagagccccg
    17761 gaaactcgaa gtgtatgtca aacatcagaa cttaaattgg aggaaaataa cgtgcgtaca
    17821 acatatatgg ccaaaccagg cgaagtagaa cgtaaatggt atgtagtaga cgcaactgat
    17881 gttccattgg gacgtctttc aacagttgtt gcatctgtac tacgtggtaa aaataaacca
    17941 actttcacac ctcatttgga tactggcgat ttcgtcatcg tcatcaatgc agacaaagtg
    18001 aaattaacag gtaaaaaagc gactgacaaa atttattacc gtcacagcaa ctacccagga
    18061 ggattgaaat caatcactgc tggcgaattg cgtgctaaaa attctcgtcg tttgatcgaa
    18121 acttctgtaa aaggtatgct tcctaaaaac actttaggcc gcaaacaatt tacaaaattg
    18181 aacgtatacg gtggagcaga acatccacat gcagcacaac aaccagaagt tttagacatc
    18241 acaaacttaa tctaatagga gggaatttca ttggcacaag ttcaatatat cggcacaggc
    18301 cgtcgtaaaa acgcagttgc tcgcgtacgt ctagtaccag gaactggcaa aattactgta
    18361 aataaaaaag atgttgaaga atacattcca catgctgact tgcgtgaagt aatcaatcaa
    18421 cctttcgctg ttactgaaac aaaaggcgca tatgacgtat ttgttaacgt aaacggcgga
    18481 ggatacgctg gacaatcagg agctatccgt catggtatcg ctcgtgcatt gctagaagtt
    18541 gatcctgact tccgtttagc tcttaaacgt gctggcttac ttactcgtga cgcacgtatg
    18601 gtcgaacgta aaaaaccagg tcttaagaaa gcacgtaaag cttcacaatt ctcaaaacgt
    18661 taatatcctt tatatatcaa cgtttctggc acttttcaat tatttggaaa gtgcttcttt
    18721 tgtttttaag aacacattta agaacacata tcaatcacac ttattcaatc ctctctgaaa
    18781 agcttcaaaa acagccttat caactatatc tggtgtgttc acatatatct ttgttgtaga
    18841 aatatctgag tgtgttaaag catttgaaat ttgattcata tctgctccac cttgtctagc
    18901 tatagttgca taagtatgac ggagtttgtg tggagataaa tgaactaagt caggatgcct
    18961 tcttttaatt gaattgattc gatagtttag gtaatcggca tgaacaggac aatttacttc
    19021 tccagatggt ttagtataag taaagagata ctgatctaac gtttgcttaa tttttaattt
    19081 caacaattga tcagcttgta ccgatttcca ttcacttagc aatgtcatta caacttctgg
    19141 tacttgaatg acggtatctt ttcgaccttt tgtaaacttt tttctttgat atttatctaa
    19201 agtatgttta agacggaccg tttgtttttc aaaatcaata tatttccatt gcaaagcgta
    19261 agtctcactt tttctatcac caagatacag ggtaatcata aaaagtgtgt aatcatgaaa
    19321 agtaagtaaa tgattattaa aatcatcatt tactgcttct atccaattag taagttcttt
    19381 tgatgatagt gcttgcttac cttcttgtat tcttttttct tctaaagcat tttttttagg
    19441 tgcagtgata ctttgaataa ttttagtagt tcgattaaat tcgatataat ttagtatttc
    19501 tgcaatatcg aacatggaac gaacatatcc ttttatgatt tttatatttg catattcttt
    19561 cgattttttg gttaattcat ctgatattat ctcagtattg atatttagat aattcattgc
    19621 gtattcacca aacataggta aaatatgcaa ccgaaatata tcttttgtat tactaatggt
    19681 tatatcacta ggaggcctgt ttgatctaat tgtttgacct aattcatatc taggaagcca
    19741 tttagattcg taaaattttt taaacgttat ttttcctttt aattctaatg agtttgcatt
    19801 ctcctctctc aaaacttttt ctattttctt cttcatatca ttttcggctt taatcgccaa
    19861 ttgcctattt ggaaatgttt tttgatactt ttctgatgca attcctgtta tttctttaaa
    19921 cctttttgga tatctcagat tagttttata tttcccattt gaaagctttg taggcaaaat
    19981 cctcactcct taaaaaaagt aaggcagtat atttcactaa ttttagtata tgcggcgaga
    20041 agaaaatttc aagttatttt aaatgtattc ataaagtctt taatatcttc tacgtcaaag
    20101 cgaactacac caccaactct tattactggg aaattatata tacgaatcca cttatctaga
    20161 gtattattat cgattcctag aaatgcacag gtttgttttt tattcaggta gactctggat
    20221 tctataaaat catctcctaa aatagtagtt atgaaatttt tgaattgctt tgggtaagca
    20281 cataggaatt tcacctctcc ccagtttacg gcgagcctta cggaagtatc attatttgct
    20341 gctattgtca tggcattttg ttttcccaga acaaaaagaa atctctaagt atttgtcgct
    20401 aatcaggtca tggcgtactt gagtttctag ctcgaatagc agggatcgtt attcaaaaat
    20461 tgtatttaat tgtcaatgat caaaaatagt ttttttccta ttcactatta ataacgataa
    20521 acatgtataa aaagacacac tacgaaaaaa gttaaatgga gtttactgat tttcatattt
    20581 gtatatggaa cagacatagt cttgaataat ttttataaat gttttgatgc ttatcaactt
    20641 attcaacccg ctatccactt caagtataat ttttccatac gccaataaag tagttttagg
    20701 agcatgttct catttgagaa cgctcctttt ttatatattt tgaaagctac ttactatacg
    20761 cgtagtttca aatagttttg tcattctaat cttcagcaac agaaaaaaat ctaaactata
    20821 aaatttaggg ttgactaaaa tataattttt gtataccata t
//
LOCUS       DAXLPI010000046        20596 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXLPI010000046 DAXLPI010000000
VERSION     DAXLPI010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.046"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            62..1693
                     /locus_tag="ACF3T5_001424"
     CDS             62..1693
                     /locus_tag="ACF3T5_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFD3632037.1"
                     /translation="MFKKYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1755..2396)
                     /locus_tag="ACF3T5_001425"
     CDS             complement(1755..2396)
                     /locus_tag="ACF3T5_001425"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFD3632038.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2582..2658
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2678..2986
                     /gene="rplU"
                     /locus_tag="ACF3T5_001426"
     CDS             2678..2986
                     /gene="rplU"
                     /locus_tag="ACF3T5_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFD3632039.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3013..3357
                     /locus_tag="ACF3T5_001427"
     CDS             3013..3357
                     /locus_tag="ACF3T5_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFD3632040.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3370..3663
                     /gene="rpmA"
                     /locus_tag="ACF3T5_001428"
     CDS             3370..3663
                     /gene="rpmA"
                     /locus_tag="ACF3T5_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFD3632041.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4097..6904
                     /locus_tag="ACF3T5_001429"
     CDS             4097..6904
                     /locus_tag="ACF3T5_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFD3632042.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7095..7586
                     /locus_tag="ACF3T5_001430"
     CDS             7095..7586
                     /locus_tag="ACF3T5_001430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFD3632043.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7689..8678
                     /locus_tag="ACF3T5_001431"
     CDS             7689..8678
                     /locus_tag="ACF3T5_001431"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFD3632044.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8711..9514
                     /locus_tag="ACF3T5_001432"
     CDS             8711..9514
                     /locus_tag="ACF3T5_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFD3632045.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9535..10449
                     /locus_tag="ACF3T5_001433"
     CDS             9535..10449
                     /locus_tag="ACF3T5_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFD3632046.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10532..10906
                     /locus_tag="ACF3T5_001434"
     CDS             10532..10906
                     /locus_tag="ACF3T5_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFD3632047.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11045..11290
                     /locus_tag="ACF3T5_001435"
     CDS             11045..11290
                     /locus_tag="ACF3T5_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFD3632048.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            11462..12526
                     /locus_tag="ACF3T5_001436"
     CDS             11462..12526
                     /locus_tag="ACF3T5_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFD3632049.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            12772..13197
                     /locus_tag="ACF3T5_001437"
     CDS             12772..13197
                     /locus_tag="ACF3T5_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFD3632050.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13190..13639
                     /gene="nusB"
                     /locus_tag="ACF3T5_001438"
     CDS             13190..13639
                     /gene="nusB"
                     /locus_tag="ACF3T5_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFD3632051.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            13736..14590
                     /locus_tag="ACF3T5_001439"
     CDS             13736..14590
                     /locus_tag="ACF3T5_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFD3632052.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            14591..15928
                     /gene="xseA"
                     /locus_tag="ACF3T5_001440"
     CDS             14591..15928
                     /gene="xseA"
                     /locus_tag="ACF3T5_001440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFD3632053.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            15933..16157
                     /locus_tag="ACF3T5_001441"
     CDS             15933..16157
                     /locus_tag="ACF3T5_001441"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFD3632054.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16159..17049
                     /locus_tag="ACF3T5_001442"
     CDS             16159..17049
                     /locus_tag="ACF3T5_001442"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFD3632055.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17058..17876
                     /locus_tag="ACF3T5_001443"
     CDS             17058..17876
                     /locus_tag="ACF3T5_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFD3632056.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            17983..18432
                     /locus_tag="ACF3T5_001444"
     CDS             17983..18432
                     /locus_tag="ACF3T5_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFD3632057.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            18450..20132
                     /gene="recN"
                     /locus_tag="ACF3T5_001445"
     CDS             18450..20132
                     /gene="recN"
                     /locus_tag="ACF3T5_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFD3632058.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20267..>20596)
                     /locus_tag="ACF3T5_001446"
     CDS             complement(20267..>20596)
                     /locus_tag="ACF3T5_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3632059.1"
                     /translation="LLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDG
                     FREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI
                     NQLIKVR"
BASE COUNT         6653 a         3461 c         4617 g         5865 t
ORIGIN      
        1 tgaaatatga tattctaatg aatggtattt tagaaaattt catgaaaaag gagttagata
       61 ggtgttcaaa aaatatgttt cctcactaaa aaaagaattt tcaggttatg atctttcaag
      121 atttcagaaa gatcttttgg ctgggatcac agtagctgct gtagcattgc ctttagcctt
      181 ggcttttggg gtatcaagcg gagctgatgc ggccgcaggt ttgattacgg ctatcattgc
      241 cggtatcgtt attggcggtt tatcaggcgg tttttatcaa atatcaggtc cgacaggtgc
      301 gatggcagct atcttgatgt ctattgcggc aacaaaggga atgcaaggtg tattgcttgc
      361 tactttttta gcgggtctat ttttacttgc agcaggactt ttccgattag gaacactgac
      421 ttcttttatc ccagcacccg taattacggg gtttacttca ggtattgcta tcattattgc
      481 attaggtcag atcgataatt tatttggtgt tcactcggaa ggtacaaacg ttattgaaaa
      541 actaagtagt tatcaaacga taggatttcc gataaacaca gcctctttga tcatgggagg
      601 gctagtcatt ttagggatgt ttatctatcc aaaaaaatgg gcgaaacgaa tgcctagttc
      661 gctgctggct atcattttgg caacagcagt tatgctattg ttccatttac cagtagcaac
      721 tgtgggtaag attccacaaa cattgatcag tagtaatcgt ttgaatatgg gagattttag
      781 cttttctgcc ttgcaacaag tggcagttcc agctattagc attgctttat taggaatgat
      841 tgaaagcttg ctatgtggtg cttcggctgg ccgaatggca aataagccct tagatagcaa
      901 tcaagaatta atggcgcaag gaattggaaa tctgttgctt cctttctttg gcgggattcc
      961 tgctactgcg gccatcgccc gtacaagcgt ggccatcaaa tctggtgcac aaactagatt
     1021 agctggtatg atacatgcag ttgtcttact gctctccatg cttgtttttg cgccagtgat
     1081 gtcacagatt ccaatgccag cacttgcagg tgttttgatt gtgacggctt ggcgtatgaa
     1141 tgaatgggaa acgatcaagg agctatttac taataaatat tggtcagcag ttctattgtt
     1201 tatccttacg atgatttgta cagttatctt tgatttaagt attgctatcg tgatcggggt
     1261 gatcggcggc tgtgtatttt ttgttgtcaa aagcgctgct atcacgattt cattagaaac
     1321 gatcgattgg caaagaatgg ggctgccaga aacggaaaag ctgaataact ggacagtagt
     1381 gtatattagt ggtccactgt tttttatgtc ttcagaaaaa ctcaaaaata aattggaaga
     1441 actaaatcat cacgatggtg tcattttctc catgcgaggc gtaccaagta ttgatttgac
     1501 ggcacgtgca gtttttgatg aatttcaaga aaaagcagag caaagaggac aaacgattat
     1561 ctatacttcc ttgcagccag aagtagaaaa acaactttct cacttgtggg aacatacatt
     1621 cacgcatcaa catcaaacag ttgctcatgc gttgacagct ttacatgcga aatatactgc
     1681 aattagccat taaaacaatg aaaaagaaca gacaagactg taaactgaag attttatggt
     1741 cttgtctgtt ctttttaata ttgctgtaaa agtgaagcta gtacttgcca gtcttgatgg
     1801 ataacaggta caagggagcg attgtagaaa atagctgctg gatgaaaaag tggaagaatt
     1861 cgatagtttt tttctgacca ttcgtaggca tccttttgtt ctgttaattg taagacaggc
     1921 cctgtataga gttgaccatg atttttggta accgtatatt ctttgccaag taaacgttga
     1981 agaccaatgt ttccaagtgt cgcaattaat tttggctgga ccttcatcag ttcgtaatcc
     2041 aatattggtg catgcgcaaa tacttcggaa cgagtaggtg tccgatttgg ataaacagtt
     2101 tctgactgtt gtgtccgttt attgatccga tgagtcacgc gatacgggcg gctccttaca
     2161 gcactggtga tatacacctc ttctcgtgtt aagccagttg aagaaagtgc ctgcatcaat
     2221 tcttttccag cttgaccgct aaaaggaaca tgcgtatcaa tctctgtttt ccctggggct
     2281 tctcccacga gcatcagttt aggatgaaga ggtccttgcc cagcgataaa tccttctaca
     2341 ggacggccgt tcattcttaa agaagcttct tgtatcagtt cttttgggta ttccatttat
     2401 gctcctcctt tttcttttat tataagaata aaagaaacac aatgcgacta ttttatttgg
     2461 tagaactgtg aggatagaaa agcagaagta ttgcttgaat ttttgtttta ctttttgatt
     2521 ttaaatttag ttgactaacg gttgctgttc tggtaagcta gtactgttgt aattgtggac
     2581 tccacagcta caaccgctca gaacaagtaa taagcacagc atatgctgtc acttcggatg
     2641 gcgagtctaa gtatatgaga aggaggtgct ttatagcatg tacgcaatta ttaaaactgg
     2701 tggtaaacaa gtaaaagttg aagtgggaca agcaatctat gttgaaaaac ttgacgttga
     2761 agcaggagaa aaagttgttt ttgacgaagt tatcttagta ggcggagaat ctacaaaagt
     2821 aggcgctcca actgttgcag gtgcaactgt tgaaggtact gttgaaaaac acggcaaaca
     2881 aaagaaagtc gttactttca aatacaaacc taaaaaacat tcacaccgta aacaaggtca
     2941 tcgtcaacca tatacaaaag tggttatcga cgcgatcaac gcttaatagc gtttgagaaa
     3001 gaaggcctat atatgatcaa aggaacattt aagcgaaatg acgcaggtca gatcgtttca
     3061 ttcactttga ccgggcatgc tgatgctggt ccttatggaa gtgatatcgt ttgtgcgggt
     3121 gtttctgcgt tagcgatcag cactgtcaac gggattgcct cattagccgg ttttgagccg
     3181 attgttgaaa tgaatgaaga agaaggcggt tatctttata ccgaagtcac ttcaggaatg
     3241 acacaagaac aaaacaatat tgctgagatt ctattagaaa atcttttatt aggattacaa
     3301 tcgatagaag ctgaaaactc agaatatatc caaattaaaa caattaatga taaataggag
     3361 gtgcagacca tgttgttatc tatgaatcta caattatttg cccacaaaaa aggtggcggt
     3421 tctacatcta acggccgtga ctcagaatca aaacgtctag gtgctaaacg tgctgatgga
     3481 caaacagtta caggtggatc aattctttac cgtcaacgcg gtacaaaaat ctatccaggt
     3541 gtaaacgtgg gtatcggtgg agacgacact ttatttgcaa aagttgatgg cgtagtacgt
     3601 ttcgaacgta aaggccgtaa caaaaaacaa gtgtctgttt atccagtagc tcaagaagct
     3661 taattgaatc ttatgattaa agaggttgtg acaagacttt tgtcacagcc ttttttgttt
     3721 ataaaaataa aagttgtagt ggaggctacg aagtttactt tgtcttttta cacttaatat
     3781 ttttttgcca atttggaatc aggatgtttt gataactatc gttttcaatt gatggaagct
     3841 atattgtttg ctatcagtga aataattata tggtaaacag tgttcttttt cttgtttcca
     3901 ttataaaaat cgtatcggga tttttcgttc ttttaaattg aacatttcta tctatccgcg
     3961 tgttatcaac aaaacaaaat caggtaaata gttgacgaaa cagaaataaa tacatagtat
     4021 tatgttttga agcctaagta caatagtcta tagaaaagga gcgataccat tggcaaaacg
     4081 cattgaccga atttatatgt atgtcaaaga aaaaaccgct catttaacaa aggcagaata
     4141 tgatcaagga gtaacaacac aggaaattgc tgaagtgtta gggattcagc gaacaaatag
     4201 cagtaaagat ctgaatcagc tagtgcgcga aggaaaactg ataaagacag acggacgacc
     4261 agtacgttac atatatcaaa cactcgttac gcacgtaaag ccagttacaa aacatgtagt
     4321 cagttacaaa gaaacgccgg tctctgaaga aaaaaataca ctccaaatag atactaagga
     4381 tatctttgca aaaatcattg gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa
     4441 agctgcgatt ttgtatccgc caagaggatt aaattgtttg attacaggac ctaccggatc
     4501 aggaaagact tattttgcac acgcgatgta tcattttgca aaagcgcatc atgtaataga
     4561 tgaggaaacg gaactgattg ttttcaattg tgctgattat gccaataacc ctgagctttt
     4621 gatgagtcat ttatttgggt acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat
     4681 ggggattatc gatcaagcgg atggcggtat gctgtttttg gatgagattc accggttgcc
     4741 gcctgaaggt caagaaatga tcttttactt tatggatcat ggaacttaca gtcgtcttgg
     4801 ggaaacaacg aaatcccatg aagcaaatgt acggatcgta ggtgcgacca cagaagaccc
     4861 aggttcttct ctattagaaa cttttgttcg gcgtatccca atcaatatca aacttccggc
     4921 ttttgaaaaa cgtcctgcaa atgagaaagt cgatttagta aaaatcatga ttgcccacga
     4981 agcaaaccgc acgcagcgaa agatttcttt gacagaagat gtagcaaaag cattgatcgg
     5041 cagtgtgacc tatggaaata taggacaatt gaagtcaaat atccagttag tatgtgcacg
     5101 aggatttcta aatcatatgc aatcacccga aatctcgatc acaatcgatg atttgacaga
     5161 agggattcgt agcggattga ttcaattagc aagcaaccgc agtgcaatgg cagagctttc
     5221 taaaatattg gagccaaaga taactgtagc accaaatgat acgatcatga aaatccaatc
     5281 ggactcctat gagttacctt ataatctata tgatatcatc ggagataaag cagctttact
     5341 gaagtcggat ggtttggatc aagaagcaat caatcatttc atttctacag atatcaacat
     5401 ccatctgaag tctttctata aagaccatgg tttttctttt aatgcagaca gcaaattagc
     5461 agaatttgta gatccgaaaa tcatagaagt gaccaatcag atttatgtaa tggtcaaaag
     5521 atctttacca tatgagtttc agcaaaactt tatttatgct atgagcttgc atatcagttc
     5581 ttttttgaaa aaaatcaact taggagaaga acggcatacg aatgataaca tccgtgagat
     5641 ggcttttgat tttccagatg aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac
     5701 gtattttcaa gtggagattc cagaaagtga agactattac ttgaccgttc tactcgtctc
     5761 gttacgggca aatcaagcaa gcggcagaat cggtgtagtc gttgcagctc acggaaacag
     5821 cacagcaagc agtatggtcc aagttgtcca acagttgtta gatgcagaga acgtacgagc
     5881 agtagacatg cctttggata tggatccgaa aacagcattg gaccgaattg aacgcaatgt
     5941 acaagaagta gatgaaggaa gcggggtcat actcttagta gatatggggt cccttgcgtc
     6001 gtttaattcg caaattcaaa gagacactgg aattccagta cgtactgttg atatggtaac
     6061 aacttctcta gttttggaaa ctgtacgaaa ggtctcagta ttagggacag atctagatat
     6121 gttgcatgaa tcattgaaaa attttcgagg ttacgcagaa atatcttctg tagaatctgt
     6181 tcaggaagca aaacctattc ataagaaaaa agctgttttg gctatctgcg catctggcga
     6241 aggtacagca caaagaatca aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac
     6301 agaattagaa gttctagctt tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca
     6361 aaaagaatat cagatcatag ctactactgg gattactgat ttgaatatcc aagccccatt
     6421 tattccttta gaacgtttta ttgatcaaaa tatcgaagtc attttggatc agttgctgat
     6481 ggaatcagaa ttggaggaaa ctgaattcat ctccctagat gaagagtcag ctaaaaatac
     6541 gtgtgttgag tttatctctg ataacttcat ctttattaat ggtagtaagt taatcgatcc
     6601 aatgtggcaa ttttctacac aaatctctca gacgacggga atcggcgatg aagaatatgg
     6661 attcaaaatc aacctagtga tgcacacagc aggaatgatc gaacgtatta ttcggaatga
     6721 accattgact gtggaagaaa acgaattgac aaatacaacg aatgatcctc tttatagcca
     6781 attggcagcc tcagttgttc tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga
     6841 aatgtattat ctgctacgtc tagtccataa tcagttagat aaaaaagaat acactgtgcc
     6901 atgaatacac aaaaacgata cacttttata gtgtatcgtt tttgtgtatt ggttaagaat
     6961 cctttaaatt caattagtgt atcgtaaaaa agttggcacg ctttttgctt atagtagtgt
     7021 gtgctaggag gtggttgttc acacagaaat gataggggtt tctgtgaagt agtgaggaag
     7081 aaggagtttt gcagatggat attcgattgg cacgtataga tgatcgttta atccacgggc
     7141 aagtcgcaac agtttggtcg aagcaaacag gtgttgaacg tatcattgtg atcagcgatg
     7201 tagtcgctca agataaattg agaaagtttc tactaaaaga agctgcaccg ccggggatca
     7261 cagcaaatgt aataaccgtg cagaaaatga ttcaaatcta tcatagtgga ttattatcac
     7321 gagcaaaagt gatgctttta ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg
     7381 gtgtacagtt gaagtcgttg aatatcggcg gtatgagttt tactgaaggc aaatcaatga
     7441 ttaccaattt tgtttcagta aatcaacaag atattgattc ctttcgttac ttgaacagtc
     7501 aagaagtgga gttagagatt cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac
     7561 tactagcgaa aatcaaaaag gtgtagcaag aatggttgtt ggaaaaggtc aaataattct
     7621 gttgtaatgg ttagcaatta ttaatatttt tgaatttgtt acaacaaaag taaataggag
     7681 gtataaatat ggtagggatt atcctagcaa gtcatggaca atttgctgaa ggaatcttac
     7741 aatccggttc aatgatcttt ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa
     7801 gtgaaggtcc agatgacttg agagccaaat tggaagaagc ggttgcttct tttgataatc
     7861 aagacgaagt gttattctta gttgatttat ggggcggaac accgttcaat caatcaaaca
     7921 cattattcga agagcacaaa gataaatggg caatcgtcag cggtttgaat ttaccaatgt
     7981 tgattgaagc ctacgcatca cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca
     8041 tcattgaaac agcaaaagac ggtgtaaaag taaaaccgga agaattagaa ccggcagaag
     8101 caccgaaagc agcagttgaa gacgcacaac ctaaaggagc acttccagaa ggaactgtgg
     8161 tcggagacgg aaaaatcaaa tatgtattag cacgtgttga ctcacgtctg ttacatggac
     8221 aagttgcgac agcttggaca aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg
     8281 cagtatcaaa ggatgatctg cgtaagagac taatcgaaca agctgctcct ccaggtgtaa
     8341 aagcaaatgt tattcctatt agcaaaatga tcgaagtagc aaaagaccct cgttttggaa
     8401 ataccaaagc attgctttta tttgaaaatc ctgaagacgt attgacagct gttgaaggtg
     8461 gcgtagacat caaagaatta aatgttgggt caatggcgca ttcggtcggt aaagtcgttg
     8521 taagtaaagt attgtctatg ggacaagaag acgttgaagc gtttgagaag cttgaacaaa
     8581 aaggtgtgaa attcgatgta cgtaaagtac ctaatgattc acgcgataac atggatgata
     8641 ttttgaaaaa agcaaaagct gaattagcaa aagcgtaata aataatatta aaatttatag
     8701 gaggcttatc atgtctatta tttcaataat tttagtcgta ttagttgcct ttctagctgg
     8761 tatggaaggt attcttgatg aatttcaatt tcatcaacca ctagtggcat gtacattgat
     8821 cggtttagtt accggtaacc tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat
     8881 cgctttaggt tgggcaaata tcggtgccgc tgtagcacca gatgctgcat tagcatcagt
     8941 agcatcagca attattttag ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat
     9001 cgcgattgcc gttcctttgg ctgttgcagg gttgttctta acaatgatcg tacgtacaat
     9061 cgctgttcca atcgttcatt tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt
     9121 tgaaatgtgg cacatcattg ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc
     9181 agcgttgcta tttatcccag cacaaactgt gcaatcattc cttgaatcaa tgccagcatg
     9241 gttaacagat ggtatggcta tcggtggggg aatggtcgta gctgtaggtt acgctttggt
     9301 aatcaacatg atggctacaa aagaagtatg gccattcttc gttattggtt tcgttgtagc
     9361 tgcaatttct caattaacat tgattgctct aggtgcttta ggtgttgcat tagctcttat
     9421 ttacttgaac ctttctaaaa tgggtggttc atcaaataat ggaggcggaa gcaactctgg
     9481 tgacccttta ggcgacattt tgaatgacta ttaatcttga aggaggaaaa taaaatggca
     9541 gaagaaaaaa tcaaattatc aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc
     9601 attcaaggtt catggaacta cgaacgtatg caaaacggtg gctgggcatt ttctatgatt
     9661 ccagcaatca aaaaattata caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac
     9721 ttggaattct ttaatacaca cccatacatt gcttctccaa ttttaggggt tacattggct
     9781 ctggaagaag aacgtgcgaa tggcgcgcct gttgatgatg tagcgattca aggggttaaa
     9841 gtaggtatga tgggtccttt agctggtgtt ggtgacccag tgttctggtt tacagtacgt
     9901 ccaatgctag gtgcactagg tgcttcatta gctatgggag gaaatatcct tggaccaatc
     9961 atcttcttct taggttggaa cattattcgt tgggcattca tgtggtatac acaagaattt
    10021 ggctacaaag ctggttctaa aattactgat gacctttctg gcggattgtt acaagacgta
    10081 acaaaaggtg cttcaatttt agggatgttc gtattagctg cccttgtaca aagatgggta
    10141 tcaatcaaat tcttaccagt tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa
    10201 tgggataaat tgccttcagg tggtgaagga atcaagagtg catttgaaca agttaataat
    10261 ggcttagccc tttctcctga aaaggtaaca acattacaag acaacttaga ccaattgatt
    10321 cctggtttag ctgcattagg attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt
    10381 tctccaatcg ttatcatcct tggtttattc gtagtcggtg ttgttggaca cgtcatcgga
    10441 cttttataat ccataaaaat atagctcggg cgttgtcccg agcttttttt atatagtata
    10501 ctgttcataa acaaatatct ggagggagaa aatggttcaa tcaatcaata caaaagtaga
    10561 cttggtaatt gatgctactt cgtttacggg tctgacagat tacgggcaaa tcatgatagg
    10621 tgataatggt tttgagtttt accatgcgcg agatagacgc aaatatatcc aaattccatg
    10681 ggaagaagtg gattatgtac tagcatcagt catgctaaag ggaaaatgga ttcctcgata
    10741 tgccattcaa acgaaaaaaa atggtacata cacattttct tccaaagaac ctaaaaaagt
    10801 gcttcgtgcg atcagagaat atgtagatga agatagaatg gtacaatcat tgagtttctt
    10861 tgatgtagtg aaacgtggta ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac
    10921 ttctaagagg aggttttctt tattttttta ctttgcagaa gaaaatatga aatagcaaaa
    10981 ccaattattt gtccccttta aatcccatat gctaaaattg attagcaaag aaggaggtta
    11041 tattatgagt tggttatgga cattgattgt tggagccgtg atcggagcaa ttgcaggtgc
    11101 gatcaccagt aaaggaaatt cgatgggctg tgtaacgaat atcattgctg gactagtcgg
    11161 ttcaagtatt ggacaagcga ttttcggcga atggggacca aaagctgcag gtatggcact
    11221 tattccatct attttaggcg cagtgatttt ggtagcagtt gtctcatttt tctttggaaa
    11281 acgatcataa gtttttacaa aatagtaaac taaaaggagt tgcagcgaga ctgtgactcc
    11341 tttttggctt tatttattta aaataaaaag aagttttccg gaaattttta tttaactgtc
    11401 gtgtacgaag aaaagtttgc tataatggaa aaaagcgtgc aaagaaagag ggagtttcat
    11461 tatgatgcta cgtgtagaaa aattgcgcaa aaaaatgcaa gaagaaaatt tggattcatt
    11521 tttggttacc agtccgtaca atcttcgtta cttaacgaac tttacaggta cgacaggatt
    11581 agctgttatt actttggaaa aagcattttt tattactgat tttcgttata ccgagcaggc
    11641 ggcagcgcag gctcaagggt ttgaaattat taaaaatgtc ggacctattt ttgatgaggt
    11701 agcaaactta gtacgcaaag aagaactgca ctcattagct tttgaggaaa caacagtctc
    11761 atttttagaa tattcggttt tagaagaaat catcgatgcg gaattagtac ctgtttcaca
    11821 gatgatcgaa gaactcagag aagtgaaaga tgaagaagaa atcgctatta tcgaaaaagc
    11881 atgtcatatt gcggatatgg catatgatca catcctgaaa atgatccaac cgggcatgac
    11941 tgagattgaa gtcgcaaatc aactagactt ttatatgcgt tctcttggcg cttcaggtgt
    12001 ttcttttgat acgatcgttg ctagcggatt gcgttcggct atgccgcatg gtgtagcaag
    12061 caaaaaaatc atcgaacaag gtgacttgat cacattggac tttggttgct attacgaagg
    12121 gtacgtttcc gatatgacac gtacattcgc tattggtgat cctggggaaa aattaaaaga
    12181 aatttatcaa atagtcttag atgctcaatt agcagttatt gatgctgcaa aaccaggaat
    12241 gactggtgtc caattagatg cagttgctag agattatatt tcaaaacatg gttacgggga
    12301 agcatttgga cattctacag gacatgggat cggtttagag atccacgaag gaccaaacgt
    12361 ttcttctcgt gcagaaaagc aatttgtacc aggaaatgtg atcactgatg aaccagggat
    12421 ctacttacca ggaatcggtg gagtacgtat tgaggatgac ttgttgatca cagaagatgg
    12481 aaatcgtgtg ttgactcgtt caccaaaaga attgatcatc ttatagtttc atcaatagaa
    12541 gaagtaagcg taaaaatcag tgttcacaga ctttgttata gaaaatcact cgatttaatg
    12601 atacactata gaagaacttt taagctgtaa gagataataa agttgggtgt tttctccaat
    12661 tcatagcagt ttgcagctga tcggcggcag ttttcttacc acgtgatcac tatgccgcat
    12721 catagagaat tcggatgtat ctcgtgctta ggcaaaggga ggaataaata aatggcagat
    12781 gaaaaaaatt tgattttaaa cgcaaaccaa gatttaggag agatcgtgat cgctcctgaa
    12841 gtcatcgaag taattatcgg gattgcagct tctaaagtag aaggcgtata cggaatgcgc
    12901 ggaacttttg ccagcaatgt cactgaatta ttaggacgtg cagctcacgg aaaaggtgtt
    12961 tacttagcaa acgaggaaga tggcttgaaa gtagatcttt actgttatct agaatatggt
    13021 gtttcagttc ctaaagtagc aatggaaatg caggaacggg tcagccagca agtccttttc
    13081 atgacggata tcgatttatc tgaagtcaat atccatgtag tagctgttgt tcctgaaaaa
    13141 ttagaacaac caacaatcga agaacttttt gaagacgaag aggaagagaa tgaataaaga
    13201 attatcaaga cacgagattc gggaaatggc attgcaggct ttatttccat tagacttcaa
    13261 tgcagaccta acaaaagaag atgcgatttt caacgcaatc gaattagacc accgcgacat
    13321 gatcaacgaa gatgaatcag agtttgttcc tgtttatctt gatacgctag ttggcggtgt
    13381 ctgtgcaaaa aaagatgaat tggataaagt gattgaaaaa catttaaaaa acaattggca
    13441 catcaatcgg atctcaaaaa tggatttggt gatcttgcgt atcgcgattt ttgaaatgac
    13501 ttatgtgaca gatgtaccag cggctgttgc tttaaatgaa gcaatcgagt tggcaaaaac
    13561 atttagtgat gaccgttcac gtaaattcgt caacggtgtt ttatccaatg tattgaaaga
    13621 attagaagct ggggcgtaag tcctagcttt tttctatgaa tcatcataaa cctgttataa
    13681 atcgggaaaa ttctatggta gaatgaaaac acaaaaaata tctgggagtg gttagatggc
    13741 agaattgatt aacggaaaag aattagctga aaaaatgcag gcagagattg ctgaaaaaat
    13801 ccaaaaattg aaagataatg gtatccatcc cggattagtc gttttattgg taggggaaaa
    13861 tccagccagc caaatctatg taagaaataa agaacgtgta gcaaaagaaa tcggaatcca
    13921 ttcattggta gagcgttatc ctgagacgat ttctgaagac gaactacttg cagaaattga
    13981 aaaatacaat caagatccta gttttcatgg tatattggtc caattaccat tgccaaagca
    14041 tatagatgaa gaaaagatcc tgttagctat ccgccctgaa aaagacgtag acggttttca
    14101 tccgatgaat ttaggcagac tccttgcggg taaaccggac aagattcctt gtactccata
    14161 cggcatcatg aaaatgtttg aagcttattc tatcgatccg gcgggtaaac gagcagtagt
    14221 tatcggacga agcaatatcg tcggtaaacc gatggctcag ctactcttga tggcagatgc
    14281 aactgttact gttgctcatt ctaaaaccga aaatctggct gaactggcac aagaagcaga
    14341 tattttagtc gtagcaatag gacgtggaca ttttgtcaca aaagaattcg taaaaccagg
    14401 tgctgtagtg atagatgtag ggatgaatcg tgatgctaac ggtaagctga tcggtgatgt
    14461 gaaatttgat gaagtggagc ctattgcagg ctggatcact cctgtgccaa agggtgtagg
    14521 gccaatgact atcacgatgc tgatgtatca aaccgtaaaa tctgccgaag cttttggagc
    14581 aggtgaatag atggcacaag aatatttaac tgtcactaca ttaacgaagt atttgaaaag
    14641 aaaatttgat gccgatcctt atttagaacg cgtatattta acaggcgaaa tatcgaattt
    14701 ccgtttacgt gccaatgccc atcaatattt cagcttaaaa gatgaccatg caaaaatatc
    14761 tgctattatg ttcaaaggag cattccaaaa attacgattc cagccaaaag aaggaatgaa
    14821 agtccttgtc gttgggcgta tttcattata tgaagcggga ggttcttatc aaatctatgt
    14881 agagcacatg gagcctgatg gaataggtgc tttatatcaa gaattggaag aacgaaaaga
    14941 aaagctgatg aaggaaggcc tttttcaagg gcctaaaaaa atgctgccac gtttcccaaa
    15001 gcgaatcgcg gtccttacca gtccaagcgg agcggttatc cgcgatatca tcacgacggt
    15061 gaaaagaaga tatccaattg tccaattggt tttgttccca acagttgtac aaggaaacca
    15121 agcagcggac aatgttgtca ggaatatcca gagagtagaa gagatgggca atttcgatac
    15181 aatgatcatc ggtcgtgggg gtggttcgat tgaggattta tggccgttta atgaagagcg
    15241 ggttgctcgg gcgatttatc aagctaatac gccggtgatt tcatctgtag gacatgaaac
    15301 agataccacg atcgctgatt tagtagcaga tgttcgggct gccactccaa cagcagctgc
    15361 agaattagct gttcctgttt tgtcagaaga gttgatgcgc attcaagaac ggcaagctag
    15421 attgcagcag gcgtttgtac gccagatcca gcgcaaacaa gaacggttcg agcgtgctcg
    15481 acgttcctat gtatttcggc agcctgaaag attgtatgaa ggtcagacca tcaaattaga
    15541 ccagttgaaa cagcgattat atcaagctac acagcaaatt tatcatgcga aagaaaaaca
    15601 aacgatcgca ttaagccacc aattggaaca agtggctccg ctttatcgag ttaaagcagc
    15661 tcgccaagaa actgcttatc tagaaaaacg cttagaagag aaaatccaac aatatatgaa
    15721 acaacagcag cagcgttttg ctcaagctgt tcaatctctt gatttgttga gtccattgaa
    15781 aatcatggga agaggctaca gctatacgac gttaaatgat caagtagtaa aaagtgtcac
    15841 cgagattcat caaggggatt tgctgacgat ccattatcaa gatggaatcg ttaaaggaca
    15901 agtacaagag atagacaaag gagaataaaa atatggaaaa tccaactttt gaagagtcac
    15961 tgcaagaatt agaaaaaatt gtcatgcagt tagaacaagg agacgttcca ttagaatcag
    16021 ctctagattc gtttaaacga ggaatggaat taagcaaaca ttgccaagat acattagcta
    16081 aagcagaaga gaccttgacg aaaatgatga cagaatcaaa tgaagaagtt gattttgacg
    16141 gaaacgagga aagctgagat gagattaact gacttttcag ctattcatct acctgctgta
    16201 gaaaaagaaa tcttgtcttt tttagatgag catacaacgg atagaggact ctatgatgcg
    16261 atgagttatt cagtgaaagc aggcgggaaa agaatacgtc ccttattact tttaacggcc
    16321 gttgcttcct ttgatgagcc tattgatgtt cccgtctatc aagtagcagc tgctttagaa
    16381 atggtgcata cgtattcctt gatccacgat gatttgcctg cgatggacaa tgatgatctt
    16441 cgtagaggaa aacctaccaa tcataaagta ttcggcgaag cattagctat attggccgga
    16501 gatggtttgc ttacaggagc attccagcta atcagtatgg cacacttagg taattctcct
    16561 aagcttctgc tgcttcagca attagctgtt tgtgcgggat cacaaggtat ggttgctggg
    16621 caagctgcag atatcgaggg cgaatcgaaa aagctttctt tagaagaatt agcatttatt
    16681 catgaaagaa agacagggca tttgattcgt tatgccttgc tggctggtgg catattagcg
    16741 aagcaacctg aagaaatctt attgctactt caacgattag ccgaacattt aggtttagct
    16801 tttcagataa gagatgatct actcgatgtg attggtacaa cgaaaacatt agggaaaaca
    16861 gctggtaaag atgaacgtat ggaaaaaaat acttacccgc gtttattagg cctagaaaag
    16921 acacgtgagg cgttggagat tgagctgtta tccgccaaca agatcataga caaattagaa
    16981 gaggacgttt tttcttttga tggcgggctg atgcgccaga tgatcaagca atttgatgtg
    17041 gagaaatgag ggaaagaatg gaaaaagaac gcgtagatgt attagcggtc aaacaaggat
    17101 tatttgaaac aagagaacaa gccaaacgtt cagtgatggc tggtttgatt tacaacgaaa
    17161 aaaacgagcg gtttgataaa ccgggagaaa aaattccagt gagtagtgaa ttaagagtca
    17221 aaggtaaaaa acttccttat gtttctcgag ggggattaaa attagaaaaa gcactcaaag
    17281 catttgatct atctgttgaa ggaaaaacat tgttagatat tggtgcatcc actggcggat
    17341 ttactgatgc agctttgcaa aatggcgcaa aaatgagtta tgctttagac gttggctaca
    17401 atcagctagc ttggaaaata agacaagatc ctagagtcgt cgtaatggaa agagtgaatt
    17461 ttcgttatgc aaaacccgaa gactttacct taggaattcc agaagttgct gttatcgatg
    17521 tttcttttat ttcactaaaa ctgatgctgc ctccattaca tgctatttta aaagaagagg
    17581 gagaagtgat tgctttaatc aaaccccaat tcgaagcagg cagagaagct gtagggaaaa
    17641 acggaattgt tcgtgaccct cagacccatc aaaaagtttt agaagacatt ttgtcatttg
    17701 cggcacaagg tggatatgat gtgctggaat taagttattc tccgatcacg gggggagaag
    17761 gaaatatcga gttcttggct cacttgagga aagtacccga aagcggaaca atcaattcag
    17821 ctattaatat ggcagaagtg gtttcaaatg cacacgaaca gtttgaccat aaataaattg
    17881 taactgtgaa aatcaagtga gaagtatgcg gattcttctt tattatacca aggaaataag
    17941 ctatcattga ttaagaagaa tatgctaagg agcgaagacg taatgagaaa aaaagatcgg
    18001 catcgtttga ttactcgatt gttgagtgag caagatatcc gaaagcagga agaatttgtt
    18061 gaaatcctga aaagcaaagg aatatctgtt actcaagcta cgatttccag agatataaag
    18121 gaactgaaac tgatcaaggt accggcttca gatggcggct accgttatag cataccagct
    18181 gaaacaaacg aagatgtaaa tactaaattg gaaaaactat taaaagacgc tttcgtggca
    18241 gttgaccaaa tggaaaaatt tgttattttg aaaacattgc cgggaaatgc ttcggcagca
    18301 gcaaatttga ttgataaacg ttacaagaaa gaattatttt cgattatcaa tgatgacgat
    18361 aatgttttga tgattacacg aactgaagac caggcagttt tattgaagaa agacttcctg
    18421 cattaccttt aagttgagga gcgaatgtga tgctacaaga aatcagtatt actaactttg
    18481 cgattattcc ggaattgcgg ctttcttttc acgaaggaat gaccgcgctg accggagaga
    18541 ctggtgcggg taaatccatt attatcgatg ctttaggtct gttagcagga ggaagaggtt
    18601 caagcgatta tatacgtcaa ggagcagaaa aatgtgtgct tgaagggttg tttgaactac
    18661 caaaacaaga aggattctct gaactgatgg ttgaattagg gatcgaaaca gatgaggaca
    18721 atctgattgt tcgacgggat atgtccttaa ctggaaaaaa tgtttgtcga gtgaatggtc
    18781 atattatcac attagctaat ctgagaagaa tcggcagtta tttagtagat atccaaggac
    18841 aaaacgaaca tcaggaactg ctccagcctg aatcacatct tgctttattg gatcgttttg
    18901 gagatgtagc tttccaacag aagaaaaaga gttatcagca agaatatatc tcttatcgtg
    18961 agctggaaaa aagggtacga aaagtgcagc aaaatgaaaa atcctatgtt cagagaatag
    19021 atatgctcca tttccagcaa gaagaaatag ctgctgctga tttgcaagtc ggagaagaag
    19081 agaaattaaa agaagaaaga gaaaaactta gcaattatca aaaaattgct gatggattag
    19141 ctgcaggtta cggagcactg acgaataatg aacaaaacag tgtagatggt gttggcttgg
    19201 ccgtttcaga actgcaaggg atcgcccatc tagatgtgga gtatgaagca atttatgaaa
    19261 atatccaaag tgcttactat ttgctgcaag atgcaatcgg cgatatgagt cgtcagattg
    19321 atttattgga gttagatgag agtcgtttag aagaagttac gcaacgttta gaattaatcc
    19381 gacagttgaa acgtaaatac ggcgaatcca ttgagtcaat attggcttac tatgacgaaa
    19441 tcaccgaaga gctagcttct tctgattttt cagaaggtca attggacaaa atgaaagagg
    19501 aactagagca aaaagagctt ctacttcagc aacaagccgc agatcttcat gaagcgcgta
    19561 aaaaaatcgc aaaagaattg gaaaaatcca ttctgcatga attgaaaagt ctgtatatgg
    19621 aaaacactga gtttgaggta cgtttcttaa aagaagaaaa tagacaattg aatcatgatg
    19681 gatttgacca aatcgaattt tacattacga ccaatccagg agagccactg aagcctttgg
    19741 tgaaagtcgc ttctggtggc gaactgtctc ggatgttgct tgctttgaaa actatttttt
    19801 cttctgagca aggtgtgaca agtattattt ttgatgaagt ggatacaggt gtgagcggaa
    19861 gagttgcaca ggccatcgca gataaaattt ctcaaatatc gaaatactct caagttttgt
    19921 gcatcactca tctgcctcaa gttgctgcag tcgccgacta tcaatattat attgtgaaag
    19981 cagtgattga tggacgtacg caaacttctg tttcagagtt gaaaacaaaa gaaagagaag
    20041 aagaaattgc acgtatgctg gctggaagtg agattacaaa actcacactc gaacatgcaa
    20101 aagaactatt gaaactcgca aaaaggacct gagccacctc gcagtggttc aggtcctttt
    20161 cagcagctga tagctatatt ttatggctct ttgtcaataa ggactgatgg gttgtacaaa
    20221 atcaaatctg ggtcagaatg aacctcattc tggctcagat tttttgttat cttactttga
    20281 ttaattgatt gatcaaaaag attctaattc tcatgttctc aaatgattta aatccgtagg
    20341 atactcgttt cattgtcttt atatgggtat tcttcgcttc tatttttcca ttggtgtaag
    20401 gatagatcat cgcgttggtg atgccttctt cataggtcag aaggttttgg agcttttccc
    20461 gaaagccgtc atctaacgtt tcgggaagtt ctgccaataa ggagaaaaat aagtcagggt
    20521 ctttgtctcg aaaggcttca actaattcat gaaaataggg atacgcctcc ttcagggacg
    20581 tagaaaagcc aagcaa
//
LOCUS       DAXLPI010000047        20570 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXLPI010000047 DAXLPI010000000
VERSION     DAXLPI010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.047"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            363..1052
                     /locus_tag="ACF3T5_001447"
     CDS             363..1052
                     /locus_tag="ACF3T5_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD3632060.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1039..2247
                     /locus_tag="ACF3T5_001448"
     CDS             1039..2247
                     /locus_tag="ACF3T5_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFD3632061.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2541..3812
                     /gene="serS"
                     /locus_tag="ACF3T5_001449"
     CDS             2541..3812
                     /gene="serS"
                     /locus_tag="ACF3T5_001449"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFD3632062.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4361..5845)
                     /gene="guaB"
                     /locus_tag="ACF3T5_001450"
     CDS             complement(4361..5845)
                     /gene="guaB"
                     /locus_tag="ACF3T5_001450"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFD3632063.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6350..7045)
                     /locus_tag="ACF3T5_001451"
     CDS             complement(6350..7045)
                     /locus_tag="ACF3T5_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFD3632064.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7135..8235)
                     /gene="ychF"
                     /locus_tag="ACF3T5_001452"
     CDS             complement(7135..8235)
                     /gene="ychF"
                     /locus_tag="ACF3T5_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFD3632065.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8244..8435)
                     /locus_tag="ACF3T5_001453"
     CDS             complement(8244..8435)
                     /locus_tag="ACF3T5_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFD3632066.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8456..9346)
                     /locus_tag="ACF3T5_001454"
     CDS             complement(8456..9346)
                     /locus_tag="ACF3T5_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFD3632067.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9333..10100)
                     /locus_tag="ACF3T5_001455"
     CDS             complement(9333..10100)
                     /locus_tag="ACF3T5_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFD3632068.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10112..10825)
                     /gene="rsmG"
                     /locus_tag="ACF3T5_001456"
     CDS             complement(10112..10825)
                     /gene="rsmG"
                     /locus_tag="ACF3T5_001456"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFD3632069.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11057..12958)
                     /gene="mnmG"
                     /locus_tag="ACF3T5_001457"
     CDS             complement(11057..12958)
                     /gene="mnmG"
                     /locus_tag="ACF3T5_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFD3632070.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12975..14372)
                     /gene="mnmE"
                     /locus_tag="ACF3T5_001458"
     CDS             complement(12975..14372)
                     /gene="mnmE"
                     /locus_tag="ACF3T5_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFD3632071.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14671..15054
                     /locus_tag="ACF3T5_001459"
     CDS             14671..15054
                     /locus_tag="ACF3T5_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632072.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15222..15980)
                     /gene="jag"
                     /locus_tag="ACF3T5_001460"
     CDS             complement(15222..15980)
                     /gene="jag"
                     /locus_tag="ACF3T5_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFD3632073.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15998..16816)
                     /locus_tag="ACF3T5_001461"
     CDS             complement(15998..16816)
                     /locus_tag="ACF3T5_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFD3632074.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16813..17169)
                     /gene="rnpA"
                     /locus_tag="ACF3T5_001462"
     CDS             complement(16813..17169)
                     /gene="rnpA"
                     /locus_tag="ACF3T5_001462"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFD3632075.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17287..17421)
                     /gene="rpmH"
                     /locus_tag="ACF3T5_001463"
     CDS             complement(17287..17421)
                     /gene="rpmH"
                     /locus_tag="ACF3T5_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFD3632076.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17944..19278
                     /gene="dnaA"
                     /locus_tag="ACF3T5_001464"
     CDS             17944..19278
                     /gene="dnaA"
                     /locus_tag="ACF3T5_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFD3632077.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19476..>20570
                     /gene="dnaN"
                     /locus_tag="ACF3T5_001465"
     CDS             19476..>20570
                     /gene="dnaN"
                     /locus_tag="ACF3T5_001465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFD3632078.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQ"
BASE COUNT         5877 a         4204 c         3785 g         6704 t
ORIGIN      
        1 ttatcatgtc atttccaaca tgtcaacaac ttatttttta ttatttaata agttattggt
       61 gttcttttga acaaataata atatatcaac atcacatgtg aaagtcaata agaaaatgct
      121 tatttatcaa catttctagc ttacatgatc attggttagt tctggtcctt tttcgttcgt
      181 ctttataact ccgagagacg taaaatcctt ttgttttcag tttaatgttc atgttcttgt
      241 cttattcaca ccaactagat gtctcacctt ctaaaatttt ccttaacaaa gtgctgcttt
      301 tatctatttc tgcttttttt tgctagaata atagtaacaa tatgactctg gaggatttat
      361 ttatgaaaat tttgattgct gacgatgata aagaaatcgt tgaattatta agtatctatg
      421 ttcacaatga ggggtatgaa gcagtaaagg cttatgatgg gaaagaagca ttatctaaaa
      481 tcagaacaat ccctgatatt gaattactta ttttagatat catgatgcct gagatggatg
      541 ggatgcaagt agtgaaagag ctacgaaaag aatcccaaat accaatcatt atgctgacag
      601 caaaaactac tgatatggat aagatcaaag gactggtcgc tggtgcagat gattatgtga
      661 ccaagccatt taatccgtta gaagtaatgg ctagagtaaa gtctttatta cggcgtacaa
      721 aaatgcagat tgccccagat cagcctgatg tattagaagt aaatgcatta atcatcaata
      781 aagactctca tgaagttaaa acaatcgatg ggaaagaaat ccagttaaca gctttggaat
      841 tcggtatcct ctacttgctt gctagtcatc ctaatcgagt atttagcgcg gatgaaattt
      901 ttgagcgtgt ttggaaacaa gaaagtattg tctctgctaa aactgtcatg gttcacgtta
      961 gtcatttaag agataagatt gaagaagcaa ctggtggtga aaaagtcatt caaaccgttt
     1021 ggggagtggg ctataaaatt gacgcaagat agtaaacgag caactgaaaa acgccgtcgg
     1081 atcaatttga cttcaaaaga aatcagcgaa ttgttagcgg aaggaatcat cacgattatc
     1141 cttttgcttc tattaaatgt ttctattctg gttgttatca gttcggtgat caacagttca
     1201 ccttctttga cgaatgcgat ttgggattca aagaatattt ttgcagaacg attgaacact
     1261 gatttgttct ggaacggccg gaattttatt attccctttt tctttctatt agatattggt
     1321 gttttgtatt ggcgcttgat tcgaagatat cgccaaatgc aattgcggca tatcatcagc
     1381 gaacttcact acatcgcaaa cggaaattat gatcatcgta tcccttttga actgagtggt
     1441 gacttaagcc gagtggtaac aagtatcaat ggattggtgg atagtaccgt agcagcgatt
     1501 gaagacgaac gtaaaatcga aaaatcaaaa gatgaattga tcacgaatgt cagtcatgat
     1561 atccgtaccc cactcacgtc catcattgga tacctaggac tgatagaaga tggccagtat
     1621 cactcagaag aagatctttt aaagtatacg catactgctt atatcaaagc aaagcagatg
     1681 aaatctttag tggacgactt atttgaatac actaaggtac gccagcctgc tgtcccggtg
     1741 aatttttctg cctttgacat gatccagttg atcgaacagt tagcagctga ttttgagttg
     1801 gaagcttcta aaaaaaatat ccaaatactt gttcaatcaa aagtagactc ccttatcatg
     1861 gatggcgata ccgaaaaact tgtacgtgtc ttcaataatc ttttgacgaa tgcgttgaaa
     1921 tacggaaaag gagcaacgaa gatcgtaatc gaagtagaac gtatcggttc agaagttgtt
     1981 gcaacagtca aaaataacgg agcaatgatc ccacaacagg caatcgataa tctattcgac
     2041 cgtttttatc gtgtagaaga atctcgttca caagcaactg gaggaactgg acttggttta
     2101 gcaatcgctc aaagcattgt ggctttgcac ggcggataca tttatgcaaa atcagataag
     2161 caatggactt ctttcatcat gcaccttcca atcaagaaaa atgaaaaatt acctattgat
     2221 acacaggaag ttattgacga aagctgaaat attcgctatt cttattacaa ttattagaat
     2281 aaatgaaaaa cacagaaccc agtacacttt attctttttc agagagctga tggcaggtgt
     2341 aaatcagcaa aggataattt ggaagttcca gttcgtttac gtgccctttc gaaataagaa
     2401 aggtcggttc aacagccgtt atgtgttcaa caagtgcagc tgacgctgaa cttgggtggt
     2461 accgcgaatt tcagactgat tcgtcccaag aataatggga tggatcagtc tttttttatt
     2521 ttcaaaaagg aggaaatcat atgttagatg taaaaatgat gcgacaaaat attgaagaag
     2581 tacaagaaaa attgatgact cggggcgtaa aaaaagaaac attagctgaa ttcatggaac
     2641 tagatgaaaa tcgtcgtaag ctactagtca aaagtgaaga attaaaaaaa tatcgaaatg
     2701 atgtttctgc tgagatcgcc caattgaagc gtgcaaaaga agatgcttct gctaaaatcg
     2761 ctgaaatgaa agaagtcggt ggcaacatca aagcattgga tactgaaatt gctgaagtgg
     2821 atgaaaaatt gaaacaaatt gcaactactt tgccaaacct tccccatgat tctgtgccag
     2881 ttggaacaga tgaagaggac aatgtagaaa tccgtcgttg gagcgaacca cgtacatttg
     2941 cttttgaacc aaaaccacac tgggaaattg cagaaaatct tgatatttta gattttgaac
     3001 gtggcgcaaa agtagccggt agccgttttg tctattacaa aggattaggt gctcgattag
     3061 aacgtgctgt ctacaacttc atgcttgatc agcatattta cgaacacggc tatactgaaa
     3121 tgatcactcc ttatatcgtc aacagtaaag cgatgtttgg tacgggacaa tttccaaaat
     3181 tcaaagaaga tgttttccaa ttggagaata ctgatctaac attaatccca acagctgaag
     3241 taccattaac taactattat aatgatgaaa ttttagatgg tgcagattta cctatttatt
     3301 tcactgcact aagccctgct tttcgttctg aagccggaag tgctggtcgg gatacacgag
     3361 gactgattcg tcttcaccaa ttcaataaag tagaaatggt caaattttcg gatgtagagc
     3421 attcttacga agaattggaa aaaatgacag ctgatgcaga agatatcttg aaaaaattgc
     3481 atttgcctta tcgtgtgatg gccttatcaa ctggagacat gggcttttct gcagctaaaa
     3541 cttatgactt agaagtatgg atacctgcac aagatactta tcgtgaaatc agttcttgtt
     3601 ctaactgtga agatttccaa gcacgccgcg caatgatccg ttatcgcgat aatgatggaa
     3661 aaatccacta tactcatact ctgaatggtt caggacttgc agttggccgt acagtagctg
     3721 ccattttgga aaactaccaa aatgaagatg gttctgtcac agttccagaa gcgctcgttc
     3781 cttatatggg gaatctgaaa gttattaaat aaagaggctg tgaaaaagcc gcttagtttc
     3841 gagtattaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa ttggacttaa
     3901 taccgagaaa ctggcttttg aacaccattt atcgagaggt tgtgaaatca gcgaattaac
     3961 ctgagtatta gagaaaaaaa tggaaaaata gtccctcata ttttaccgtt tgttgaacta
     4021 ataccgaggg tcagctgatt gaacaccgtt tagtggagag gtcgtaagcc taccccccag
     4081 ctcctagtta caaggacaaa ttcaaaaaac agctcttcat attttatgaa tgttgtactt
     4141 gtgattcgga ggtggcaagg gaacactgtt tattcaaaaa gaggctggga cagaagcgtt
     4201 taactccaag aaataagaag aaattcacga aaattgcttt tcaaattttt gtgaatttct
     4261 tcttatttcc gaaggagttg cttctacttc cgccgtttat acgtgtttag agcgtgagac
     4321 aaaagtgttc tttacttttg tcccacgctc tttaatgttt ttaagattca actgaatagt
     4381 ttggtgcttc ttttgtgatt tgtacatcat gagggtgtga ttcttttaat ccactgccgc
     4441 tcatttggat aaattgcgct tcatcacgta attgctgtag attggctgcc ccaacataac
     4501 ccattcctga acgcaaaccg ccgatcattt ggaaaataat atctgcaaca cttcctttat
     4561 aagcaacccg accttcgatt ccttctggta ctaatttatt tgcttcattg acactacctt
     4621 ggaagtaacg gtcttttgaa cctttttcca tcgcgcctaa tgagcccata cctcggtatg
     4681 ttttgaatcg acgtccttgg aagatttcga attcacctgg tgattcatct gtccctgcca
     4741 acatacttcc tagcatcact gcatgtccgc cggcagctaa tgctttgacg atgtcaccag
     4801 aatatttgat ccctccgtct gcaataatcg ctttgccata ttgacgagca actgatgctg
     4861 catcataaat agctgtgagc tgaggtacac ctacaccggc taccacacgt gtcgtacaaa
     4921 tcgatcctgg tccgatacca acttttacga catctacacc tgcatcatat aaagcttttg
     4981 tcgcttcagc cgtcgcaacg tttcctgcaa tcaatgttgc atctgcaaaa gttgaacgga
     5041 tttcttgaat ttttcggatc acacctgcac tatgtccatg agctgtatcg atgatgattg
     5101 catctgcccc tgcttctaat aaagcattcg cacgctcaaa tgtatcgctt gttacaccaa
     5161 cggctgctgc tacaagcaat cgaccatgtt catcttttgc tgcatttgga aattcgatca
     5221 ctttctcgat gtctttgatc gtgatcaatc cgcttaaacg accttcgtta tctacgattg
     5281 gtaatttttc gattttatgt ttttgcaaaa tcttttctgc atcttttaga gaggtaccta
     5341 ctggggcagt cactaagttg tcttttgtca tcacatcagc aatcggcatg tgataatctg
     5401 ttacaaaacg catgtcgcgg tttgtaatta tccctaccaa ttttctgttt tcaagtgttt
     5461 caacgattgg cacaccactg atacgatatt tgctcatcaa ttcttctgcg tctgctacaa
     5521 gatgctgtgg cgttaagaag aacggatcaa tgattacgcc actttcagaa cgtttgactt
     5581 ttcttacttc atccgcttgc tggctgatcg tcatgttttt atggatcaca cctagtccgc
     5641 cttgtcttgc catagcaatg gccatcttgc tgtcggtaac agtatccata cttgcactga
     5701 tgattggaat attcaactta atatttttag ctaattgtac gctcatatcc acttcgtttg
     5761 gtaacacgtg actttctgct ggtatcaata agacatcatc aaaagtaaat ccttttttcg
     5821 tgaactttgt ttcccagttg gacattttta gtaccactcc tctaatttag tttatctatt
     5881 aaaataattg attttttcat ttaagtcaat gatctacttg gaatcaagtt agctttctca
     5941 tagaatacca atataaagcg tttacatctg tgtgtattaa aagagattgt aaaaaagctg
     6001 tcctgcatca agtaataaga acagccaatc aaaaacagct tctcatgttt ttgaattggt
     6061 tgggtttatt accgcagatg caaacttttg aacaccgttt agtggagagg ctgtgaaaaa
     6121 gctgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
     6181 aaattggact taataccgag aaactggctt ttgaacaccg tttatcgtaa aaagggactg
     6241 tgacaagact tttgtcacag tcccttacta ttatttaact atggataaac aaaatgaggt
     6301 tctttactac ccctagatat ctagcaaaat agctgatctt tttgctagtt tatcttcttg
     6361 ggacggataa acttccaata atcccgtatt ttttcttcaa gtagtaacgg attgctaaag
     6421 cagttgcccc gataatgata actactactg gatctaatac tggattgata actggtggca
     6481 atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt cgttccacct aggataagca
     6541 atgtcttacc cattcctggt ttcttgcttc gatctgctcc gggatattca tattgataga
     6601 tgtatttgta catcatataa aagacccagc caccgattgc agaacctaga acgagtgtag
     6661 aaaagccata agctggcgtt gttccacgtg aaaacaatcc ggtaatcgca atcatgatac
     6721 ttaatagacc aagcaacagc aacgtattat ctaaccacat caataaaggg gtggatgaat
     6781 tttttacttc tgctggctta ttgatgattg cttccgttct ttcagagact gtcccaaaca
     6841 attgacgggc tgttcttccg cctttttgtt ctgcaactaa agtaggcaga atgtcatgaa
     6901 gggcttttac tttttcttct tcagacaaat tcgctgcttc taaagatttt ttcaaatcaa
     6961 aaatgtactg ttgatttctt ttggttaatt ttgtttctaa actattgttt tcttttacga
     7021 ttgctcgtaa ttgttctggt tccattatct aatagtcctc cttagccgtt ccttttctca
     7081 acggtcgtca caaaagacat tctaccatat tctacagcta gtttgatagc ccttttacac
     7141 attaaagcgg aataacatta cgtcgccgtc ttgtacgaca taatcttttc cttctaatcg
     7201 aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa tgatccaagt cttcaaaaga
     7261 gacagtttct gcacggataa atccgcgttc gaaatctgaa tggatgattc ctgctgcttg
     7321 tggtgctttg attccttttt tgaatgtcca agcacgtact tcttgttccc cagcagtaaa
     7381 gtaagtagct aagcctaaca aatcataagc agcacggatc aactgatcca aacctgattc
     7441 ttcgattcct aaagcttcta aaaattccgc tttgtcttct tcatctaatt ctgcgatttc
     7501 ttcttctgca cgagcactga ccacgatcac ttctgcattt tcttctgcag caaagttgcg
     7561 cacttcttgt acataatgat tcgcgtctgc atctgctacg tcatcttctg agacattggc
     7621 tacataaaga attggttttg tcgtcaataa aaatagtgat ttaacgattt tttgttcttc
     7681 ttcagtaaat tcgatcgttc gggcagattt tccttcttcc agtacaggtt tgattttatc
     7741 cagtacagct agttctgcca ctgcatcttt atctttggtt ttagcaactt ttgctacacg
     7801 agtatagcgt ttgttgatag attctaagtc tgctaacact agttccaagt tgatcgtatc
     7861 aatatcttct agtggatcaa cacgtccttc tacgtgagta atattatcat catcaaaaca
     7921 gcgaacaacg tgacaaatag cgtctacttg gcggatatgg cttaagaatt gatttcccaa
     7981 accttctcct ttacttgcgc cttttacgat tccagcaata tctgtaaatt caaaagtcgt
     8041 cggaacagtt tttttcggat gtacaagttc tgttaagcgt tgcaaacgcc agtcaggtac
     8101 ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga tagtttgctg cttctgctcc
     8161 tgcttttgtg atcgcattaa acaaagttga ttttcctacg ttgggcaacc caacaattcc
     8221 agcagtcaat gccataaatt tattcactct ttctctgcat ttttttcaat tacttttttt
     8281 aatttctttt caaaatctcg acgggtcatc atgacgatat gtccgcaatt catacatctg
     8341 attttgatat ctgcgcccat tcgaatgact tcccagcgat tcgtctggca agcgtgaggt
     8401 tttttcattt caacgatatc gtgcaagtcg tacataaaaa tagcctcctt tttgtttatt
     8461 cttcatcaaa ttgaatattc aagatatcaa gaattcgagt caaatctttt tgagaaagat
     8521 attcaatctc gatcttacct ttcccatttt tttcttggat agctacactt gtaccaaatt
     8581 tatccatcaa acgatcttcg ctttctcgga tataatatgg tttttccttt gctagccttg
     8641 ggattttttt cttttctttt ccatcatttt gatttaactc agaaacaagc tgttccagtt
     8701 ggcggacagt taaattttca gttactgcac gattggctaa tttcaagatc aatgttttat
     8761 tttttaaacc taacagcgta cgggcttgtc ccatagataa acgctgatct tgtaccattt
     8821 ccttgacaag atccggtaaa gaaagcaagc gcaaataatt ggcgatataa ggtctgctct
     8881 ttcctaaacg actagctact tctgcttgtg ttagtttcag gtttttcatc agcatctcat
     8941 atgcttctgc ttcttctaac ggattcagat cttctcgctg caagttttcc agcactgcta
     9001 cttgcatcat cgcttcttcg tcaaagtctc taacgatagc agggatcgtt tctttttcag
     9061 ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat ctcatatcct tttactgcgg
     9121 aacggcgtac gatgatgggt tgaaagacac ctgattgttc aatagaatta gctagttcct
     9181 gcaaagatac ttcttcaaat gttttacggg gctgatacgg atttggtcgc agctcattca
     9241 atggtagctg gacaacggta tcatctttta catccaccgt ctctaagttt tctaaatctt
     9301 gaaacagtgc atcgattcct cggcctaatc ctttactttt cttcacgact taccacttcc
     9361 tttgccaatg cttggtagac ttcagctcct cgagaacgtg gatcgtagtc gatgatcggt
     9421 tttccatgac taggtgcttc tgataaacga atatttcttg gaatgatcgt ttcgtaaact
     9481 ttttcgcgga agtatttacg tacttcttct acgacttcat ttcccagatt tgttcgtgca
     9541 tcatacattg tcagtaagac tccctcgatc tctaattctg gattaaagtg tttttggact
     9601 aaacgaaccg tattcagtaa ttggcttaat ccttccaatg cgtaatactc acactgaaca
     9661 ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg tcagatggcc aagagaaggc
     9721 gggcaatcga tcaaaatata atcatactga ctgcttactt ctgctaaaga tccttttaat
     9781 cttgattctc ttgccatcat tgacgtcaat tcgatctctg ctcctgctaa ttggagagtc
     9841 gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag ttgcctcatc aattggcaat
     9901 tcattgacta gaacatcata gatgtctctt gtcacatcag gttttctgat cccgacacca
     9961 ctggtagcat ttccttgtgc atccatatcc actagcaata cttttttgcc aaggctagca
    10021 agacaagcac ctagattcac cgtcgtcgtt gttttgccga cgccaccttt ttggttcgca
    10081 acagatatta tttgtgccat ctcttctcct cctatttgat tggctgctta ttaggcagtc
    10141 cgggctttct tgggtatttc tttggggttg gtttcttttt ttgaataacg atgatatgcc
    10201 gttgatcaga gacattcggc aaggtaaccg cctcttcttt gaggaattta ccacctaata
    10261 aagcaatcgc ttgttttgct tctgtcaatt cctcttcact ttttgctgct ttcaatgcat
    10321 aaaaatagcc ttcttgttta gccaagggca aacacaattc cgctaacaca ttcaaacgag
    10381 caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa gtgaggattt tgcccaaatg
    10441 tttctgcccg gtcatgatat aaggaagcat cgattcctaa taatcgggtt aactcagata
    10501 agaaggttat tcgtttattc agagaatcaa cgatcgttat ctgcagttct ggaaaggcaa
    10561 tttttaaagg tatgctagga aaccctgccc ccgaaccaac atcgcataaa gataaaggtt
    10621 gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta aaaatgcttc agatatacgt
    10681 ctttttcatc cgtaatagct gtaaggttca tcttttcgtt ccattcgact aatgtccgat
    10741 aataaatagc gaattgattc atttgatgat cggtaagctc tatccctttt tctgccaagg
    10801 attggcgaaa ttcttctggt atcataaata ttcctttcat tgtgaaacag caattgtttc
    10861 acagattctc tccctacttt aacgcaaaat ctctagacat gcaatccttc catatggatt
    10921 cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt ctttctagta tgactcataa
    10981 aatatacaca gaagagagaa ctagctttga gcaatttctg caaaaactag ttctctcttc
    11041 tatttattat tacgtattac gctggttcac tgactttcgc aatcttccct tgttcgatat
    11101 agaccattag gatacttagg tctgcagggt ttacgccact gattcggcta gcttgggcaa
    11161 ttgtttctgg ttggattttc tgcaattttt gttttgcttc tgttgctaat ccgttgattg
    11221 ctgcgtagtc gatgttttct ggaatctttt tcgcttccat gcgttttaat ttttctactt
    11281 tttccatggc ttttttgatg tagccttcat atttgatttg gatttctact tgttcgattt
    11341 cttttcgtgt caattcttca ttttctggaa taaaccgtag aagttctgca taagtaatct
    11401 caggacgtct taaaaagtcg cttgctagta caccatcttt taatggtgcc gcattaatcg
    11461 ttgctaaaaa gtcttggact tcttctgttg gtttgattcg tacattcatc aaacgtttga
    11521 tttcttgttc aactgcttct tttttcttca aataattctt gtattgatct tcttttacta
    11581 agcctacacg gtgacccatt tctgttaatc gcaaatctgc attatcatga cgtaaaagca
    11641 gacggtattc agcacgtgaa gtcagcaaac gataaggttc gttcgttcct tttgttacca
    11701 aatcatcgat catgacaccg atgtaaccgt cacttcgttt gatcactaaa ggttcttttc
    11761 cctgaacttt caaagctgca ttgataccgg caatcagccc ttgtccagcc gcttcttcat
    11821 aaccgcttgt accatttgtt tgtccagctg tataaagatt ttcaacgact ttagtttcta
    11881 atgtcggacg gagctggtgt ggctctacta cgtcatattc gatagcatat cctgtacgca
    11941 tcatttctac tttttctaat ccagcaatag aatgaagcat gttgacttga acatcctctg
    12001 gtaaagaagt agataaacct tgaacataga cttcttccgt atttaagcct tctggttcca
    12061 aaaataattg atgacgtggt ttgtctgcaa aacgcacgat cttatcttcg atggatggac
    12121 aatagcgagc gcctacccct tcaacgatcc ctgtaaacat tggggcacga tgtaagttgt
    12181 ctcgaataat ttggtgagtc gtctcacctg tataggtcag ccagcaagat tcctgatcta
    12241 gtttataggc actatctggt gtgctgaagc taaaatgatt cggctctttg tcaccaggct
    12301 gctcttctgt aacagaatag tcgatcgtac ttgattttac tcgaggcggt gttcctgttt
    12361 tgaaccgttc gatagcaaag ccaagttctt ttagatgatt cgctagtcca atagaaggtt
    12421 gagagttatt cggacctgat gaatatttca attcaccgat aatgatttct ccgcgaagtg
    12481 ctgttccggc tgtaataaca actgctttgg cccggtagct cgctcctgtg gacgtcacta
    12541 ctccatgaca aacaccatct tcgacgatca gttcttcgac gattccttgt cttagcgtca
    12601 aatttggttc tttttcgatc gtatgtttca tttctgttga ataagcatgt ttgtctgctt
    12661 gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt caacatacgc atttggatat
    12721 aggttttatc gatattctta cccatttctc cgcctaacgc atcgatttct cgaacgacaa
    12781 cgcctttagc tggtccgcct acagaaggat tacatggcat gaatgctacc atgtccaaat
    12841 ttattgtcag taataatgtt tttgatccca tacgtgcagc agcaagtgca gcttctgaac
    12901 cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc tgcttcataa tgttccaatc
    12961 gtttgttcct ccttttattt tcctaaacaa aattgactga ataattgagt gattagctca
    13021 tcttgtacac tgtcgccgac aacttctcct agatagtccc agcaacgcgt catatctatt
    13081 tgaacgagat ccacaggcat tcctgaatca atacctgcaa tgacttcttc aagtgagagg
    13141 gaagcttttt ctaacaacgc gatatggcgg gtattagaaa cataagtagc gtctctttct
    13201 cctgtttctc cgctgaagaa taaatcagaa atagctgatt ccaaacgatc tagtccgtcg
    13261 tttttagcta cggaaattga aaaaactggt tcattttcga tcaatttctt caatttttct
    13321 tgttccagct gtgctggcaa atctgtttta tttagaagaa tgatccgttt caatccactt
    13381 gtcgcttcca ataattgttc atcttctgct gtcagtggtt cgctttggtt tagaacaagt
    13441 aagatcaagt ctgcttctgc taatgctttt cggcttcttt cgaccccgat ccgttccacc
    13501 acatcttctg tttcccgaat tccagctgta tcgattagct tgagcggtac cccccgaaca
    13561 ttgacatact cttcgattac atctcgagtc gttcctgcaa tatccgtaac gattgctttt
    13621 tcttctctta acaaatggtt taacaaactt gatttgccga catttggccg gccgatgatt
    13681 gccgtactta acccttcccg cagaaccttc ccttgcttgg atgtagcaag tagcgcttgg
    13741 attctttgtt ggatcatctg cgctttttca agcaataact tcgtagtcaa ttcttcgaca
    13801 tcatcatatt ctggataatc aatatttact tctacttgtg ctaatgtctc taaaatctct
    13861 tgtctcaatg agcggatcaa agcagacaga ttgccgtcta actgattaag agctagtccc
    13921 attgctttgt ctgtttttgc ccgaatcaaa tccatcacag cttctgcctg tgaaaggtca
    13981 acacgaccat tcaagaatgc tcgttttgtg aattcgcctg gctcagctaa tcttgcacct
    14041 tctcttaaca atagctgcaa aatttgattg actacaacga tcccaccgtg acaattgatt
    14101 tctactacat cttcccgcgt aaatgtcttt ggtgctctca tcacggagac catgacttca
    14161 tcgacaagct gattgctctt gggatcaacg atatgtccat aatggatcgt atgacttggt
    14221 acttctgata atcgtttgtt tccagattga taaactttat ctgctagttg cacagcttga
    14281 tcgccactta aacgaacgat actgattgca ccttctccag gtggggtaga aattgccgca
    14341 atcgtatcaa attctaaagt aatttcagcc attttttcac ttcctttttc attttgcgca
    14401 caaaaaaagt gcccactcca ccctttatgc acaataaacg gtgaacttcg cactttgact
    14461 gcttgttctt gattaaattc tggtcataga ttatcacact tgctataagg aatcaagtgt
    14521 ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat taccttatat ttaattgtcc
    14581 ttggatgtct ctttgcgttc tttggtttat tcgaagagaa gatactgtat aaacaaggct
    14641 ttatctttct taaaaaaaac ggtacactaa atggtagcgc tatctaaact atacactttt
    14701 gaaaggagaa aatatgtgag cccgttcaga tctgctgcat tggaagaaga aagtgaccct
    14761 cggatcaacg atgtcgtttt atctacaggg aatatcaacc agaaatatat cgtccgcgat
    14821 atcgtttttg ttgtggaatg cttagaagct gatttgtttg acccatcttt tcccaaagat
    14881 gaccttttct taacaacgaa gaggaaatta aagacgaagg tacttgaata tggtgcgaat
    14941 gcagtaatca actgtcattt tgagtatcag caaaaaaaac gcgacggaaa gtattttctg
    15001 gaactttttg cttatggaac agtgatccaa tttatccaaa caacgattgg ttaaatgaaa
    15061 cagacaaaaa aagcggatca aaactgaaga cagtttcgat ccgctttttt ctatcaaagt
    15121 tagtgactac ttcatcgtat actctgataa gaatgatttc tagtaaaata tacttggttt
    15181 gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga atcaataata ttttttagca
    15241 ggttctacta ctaaataacg ataaggttcc tcaccttccg aatgtgtttt tacatgttca
    15301 tctttgctta atgcagcatg gatttgttta cgttcaaatg caggcattgg ttctaagaaa
    15361 acaggctgcc ctgtccgttt tactttttca gccgtgcgat cagctaaacg ctgaatgatc
    15421 tcttggcgct tttcacggta attgcctaca ttgactacta cagacagctt atttcttgct
    15481 aaacgatgga tgaagacttg tgccagatat tgcaatgcat tcagtgtttt tccatgtttc
    15541 ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa caaccatatt atttgattct
    15601 ctttccattt tgacaagagc tggagcatca agctgttttg aaatttcagt aagataaact
    15661 gccaattccg ttaaagctgc ttcatcacct agctcactta cttcttctgt tttttcttca
    15721 agaatcgttt cgttgactac ctcgatctca gctgttggtt cttcttccaa aatttcttct
    15781 acagtctgtt ctactgtttc agaaactttt tcgctgatac ttggctggat cgaaagccgt
    15841 gcattctttc gacccattcc taggaaacct ttttttcctt cttccagtat ttcgatttcg
    15901 acttggtctt tggccaaacc caaatcagcc agacctagtt ggacagcttc gtctattgtt
    15961 gcagcttcat aaatcggcat tagcattacc tcccctatta tttccgtttt ttcttaggat
    16021 tcattgcttt cttaagagca cgttctcttt cgcggattct ccgtgcttct tcttcacgct
    16081 cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg tacaacttgg aaggcattgg
    16141 aaactaccca gtataatgaa agaccgctgg ctaaattgac acccatcact aaaatcatta
    16201 caggcatcac gaaattcatg atcttcattg atgcattcga ttcaatttga ctcatgctag
    16261 ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat cggcaaaata aatgttggat
    16321 ccgcgttccc taagttcaac cataagaaat gcccttgttt caactctggt acacgtgaaa
    16381 tagattgcca taacgccatc aagattggca tttgtactaa taaaggaaga caaccagcat
    16441 atggattgac gccattttct gcatataaac gctgctgctc ttctcttaac ttactttgtg
    16501 tttccggatc tcttgaacta tattgttgct gcagtgcttt taatttcggt tgtaattctt
    16561 gcgttttacg catgctcttt gtttggaaat gcatcaacgg caataggatc acgcggatga
    16621 tcagtgtaaa taaaatgatc cctatccctg cattaccaaa tgacaacgcc ttgatcgcct
    16681 cagcaaagta ataaacaata taacgatccc aaattcctgt actctgtgcg ctgacttccc
    16741 cagttccgca accggataag acgatgacca aactaacgat accggccatc aaaagtaaac
    16801 gtttgtattt cttcacttac tcttttcctc tctgtcgatt atttttgcaa gttttaagac
    16861 gtggactaaa ttggagcgaa cctcatcata agtcaacccc ttcacacttg gtctagcaat
    16921 cacaataaaa tccaactccg gatcaatttc ttcttttata gaataaactg ctgctcggat
    16981 ttttcgtttg acttcattgc ggtttaccgc gttgccgacc ttttttccta cagataaacc
    17041 aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa acgacgaatt tgcggttagc
    17101 aaaagaatct cccgtatgga acacggcttg aaactctcgt tcttttttta ctcgatacgc
    17161 ttttctcatt agttttcttt ccttttctat ctctcactta cgctcataat cataccataa
    17221 ttacgattgt cctttcatca tttccctaaa aaagagtata aaaaaaacca ctgaaacccc
    17281 agtggcctaa gctgaaagaa cttttcttcc tttgcgacga cggctagcta acacacgacg
    17341 tccattttta gtactcatac gtttacggaa tccgtgaact ttttgacgtt tacgtttatt
    17401 tggttggtat gttcttttca tatattccac ctccaaaatg ttcttatcta ttttataaac
    17461 acagacatac tttgatagta taacgacaaa gtagtgattt tgtcaatata aaatagaaga
    17521 tcgattgtta aaacttatcc acaagattat ttttttctat gggaatccta tgtggaaaaa
    17581 gttatgcaca gggattatcc acagataata aaaggctggt tttacacaga aaacacacgt
    17641 gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat tccgtctcct aactattggt
    17701 atatctgtat tttttatcca cagacgcaag aggaaatcta ttggttttta cgagttttcc
    17761 acctgtgcat tagtggtgtg gaaaactttt tttgacaact gtgtttttct cttttttctt
    17821 tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa
    17881 ctattgaaaa aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct
    17941 tatatggtat ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta
    18001 tcgcctgcta gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac
    18061 cagctcgttg ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg
    18121 acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt
    18181 actggcgaag aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca
    18241 cctagtccgc taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt
    18301 ggtaaaggaa accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct
    18361 atttataacc cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat
    18421 gcaattggtc atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt
    18481 gaaacttttg ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt
    18541 caagaatatc gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa
    18601 gaagctacgc aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa
    18661 atcgtactaa ccagtgatcg gttgccaaac gagattccga aactgcaaga acgccttgtt
    18721 tctcgctttg catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca
    18781 gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc
    18841 tacatcgcag gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg
    18901 caagcttttg ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg
    18961 aaaacgttaa aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa
    19021 gtggcaaagt attatcacat ccaattgaaa gatctcaaag gaaagaaacg cgtcaaatct
    19081 attgttgttc ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta
    19141 ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa
    19201 aaaatccagc agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa
    19261 aactttttac taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga
    19321 aaaaaacgtt caaaagttat acacactttt tccacagctg ttttttcgtt tctctcttac
    19381 gttttttgag ttttccacag aatcaacatg ccctattact tttattattc tttttaacta
    19441 ataataaata aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta
    19501 gctttatgca ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta
    19561 ttttgacagg tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg
    19621 ctgatatatc aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg
    19681 aatctactgg ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc
    19741 ctgaagaaac atttacttta gaagttttag aaaataaaca agtagcgatc acttctggaa
    19801 aagcgaattt tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg
    19861 aaagccataa ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag
    19921 tttttgctgt ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat
    19981 ctgataattc tttattagct gtagctactg attctcaccg tctaagtcaa cgcgtgattc
    20041 cagtagaaca agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat
    20101 tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag
    20161 tgttgttcaa aacagaaacg atgtatttct attcccgttt gctagaagga aactatccag
    20221 ataccaatcg tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct
    20281 ttttagcggc gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc
    20341 gtttatctat tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag
    20401 tagaagaaag cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc
    20461 cggattatat gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta
    20521 tctctgctat tcgtccattt acattggaac caacggaaga tggcgttcaa
//
LOCUS       DAXLPI010000048        19052 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXLPI010000048 DAXLPI010000000
VERSION     DAXLPI010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.048"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(64..1632)
                     /locus_tag="ACF3T5_001466"
     CDS             complement(64..1632)
                     /locus_tag="ACF3T5_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFD3632079.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(1636..1917)
                     /locus_tag="ACF3T5_001467"
     CDS             complement(1636..1917)
                     /locus_tag="ACF3T5_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFD3632080.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(1914..2837)
                     /locus_tag="ACF3T5_001468"
     CDS             complement(1914..2837)
                     /locus_tag="ACF3T5_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFD3632081.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(2834..3712)
                     /locus_tag="ACF3T5_001469"
     CDS             complement(2834..3712)
                     /locus_tag="ACF3T5_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFD3632082.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(3699..5036)
                     /locus_tag="ACF3T5_001470"
     CDS             complement(3699..5036)
                     /locus_tag="ACF3T5_001470"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFD3632083.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(5039..6142)
                     /locus_tag="ACF3T5_001471"
     CDS             complement(5039..6142)
                     /locus_tag="ACF3T5_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFD3632084.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(6216..7724)
                     /locus_tag="ACF3T5_001472"
     CDS             complement(6216..7724)
                     /locus_tag="ACF3T5_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFD3632085.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(7690..8382)
                     /locus_tag="ACF3T5_001473"
     CDS             complement(7690..8382)
                     /locus_tag="ACF3T5_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFD3632086.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(8404..9378)
                     /locus_tag="ACF3T5_001474"
     CDS             complement(8404..9378)
                     /locus_tag="ACF3T5_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632087.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            9678..10550
                     /locus_tag="ACF3T5_001475"
     CDS             9678..10550
                     /locus_tag="ACF3T5_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632088.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(11132..13330)
                     /locus_tag="ACF3T5_001476"
     CDS             complement(11132..13330)
                     /locus_tag="ACF3T5_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632089.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            13562..14554
                     /locus_tag="ACF3T5_001477"
     CDS             13562..14554
                     /locus_tag="ACF3T5_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3632090.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            14614..16035
                     /locus_tag="ACF3T5_001478"
                     /pseudo
     CDS             14614..16035
                     /locus_tag="ACF3T5_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
     gene            16028..17527
                     /locus_tag="ACF3T5_001479"
     CDS             16028..17527
                     /locus_tag="ACF3T5_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFD3632091.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            17566..18960
                     /gene="uxaC"
                     /locus_tag="ACF3T5_001480"
     CDS             17566..18960
                     /gene="uxaC"
                     /locus_tag="ACF3T5_001480"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFD3632092.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         5727 a         3880 c         3366 g         6079 t
ORIGIN      
        1 aaacaaaaaa attcactaac tagtgaaaat aagttagtga acctcattta ttaaagaatt
       61 aaattacaga tgatcttttc catcatactt gaaaaactca aaatcagcat aacttcctct
      121 ttttttattt agatcatgca ctgctatccc gataaaattt ccagtaaacc ctcctgagag
      181 tatacacatg tttttttgcg gacctacttt cttccattct tcagttgata cgtctttcaa
      241 atgaaactgt gctgtctctt tctttacagt gactttcaaa aaagtcttct tttctgtgag
      301 tggaatcgtc caatcttcta tttgtgctac tccgttttct gattctatca tacgtaggca
      361 ttttcccttg tcctcattcc aagtaacata acaataaata taattggatt catttaagta
      421 cagtaccagt ccagccatct gattaaaatt attaggcatg tattcaaatg ctgtctccgc
      481 atcaaaagta aaatccgttt gccgaatagc taacaaatga tgttcgaagg tagattgaag
      541 agattcaccg gaaatcagac gtaaaaaacc tggtctacat tttgtatcac accaatcagc
      601 atttggcaaa atgcgtctgg cattccagtt cttctttagc tctccttcaa atgtatccat
      661 aaactgatga tctttttctt gttgtacctc ttcatttgtc tcaatcatta tttcttccat
      721 cggagttgtc tggctatctt taagtcttaa ccaatcgtcg gacgtccaat acacttcttg
      781 aatagcggtt tctcttccca aaatagccgc accgtttaat ggtctggtac aaagatatgt
      841 catgtaccat cttcctgacg gggtttgtac taagctccca tgtcctgtac attgcaatgt
      901 agaagcaggt ttgtcactag ctgtcagcat aggaaaacca ggatcaagct cgtaaggacc
      961 agtaatattc tttgctcggc aaactgtaac tgaatgtcct cttcctgtac cgccttctgc
     1021 cgtcatcaaa taataatacc cgttacgaaa atacaaatgt ggtgcctccg ttttcgctaa
     1081 ttctgtacct gcaaatattt tatagatggg accatcaagc ttcttcttct ctgcatcata
     1141 ttcttgtaat acgataccgc tggatttatt cggtgtgtcc agacgataat cccatagttc
     1201 attgacaagc catttttttc ccgtttcaag atcatggaac aaagaaggat caaagccgtt
     1261 actatttaaa taaattggtt cggaccaagg acctgtgatc tcagatgcag tgatcaaata
     1321 attgtggaca tctttaaacg gacggcctgc acttttgaca tctgtgtaaa caagataaaa
     1381 caatccatca gcataactga tctgtggcgc ccatatactt ccatttgtag gatttccttg
     1441 caaagatact tgagtggtca aaatatccgt tgtatgtgac cagttgacta aatttttcga
     1501 ctcatatact cggattcctg gaagccattc aaaagaggag acaataatgt aataccgctc
     1561 tttcactctt aatatattag gatctggatt gaatcctctt aaaataggat ttttcacttt
     1621 gatagtctgc atgattcagg ctccttttct cattcttact cgatataaac tgaatgcccc
     1681 agctgctaaa aaagcaagtg aaataaacaa cacaacggca aacagaccag tcatgtcctg
     1741 taataatcca aatgtatttt ctaaagacaa agttgaatga tctacagcaa gcgactgata
     1801 acttttttta aactctgacg cagacatatt acttaaagta actgctaacc cgccaaagaa
     1861 aatcaaagat agactgccac cgaccattcc tagcaatagt tcccattttc ttttcatttc
     1921 tgaaccccct gcttttctaa atgtatccat tcaggtcttt tgatcgatgt ttcataattg
     1981 ttattacgtt cagagaattt gacagtctgt tcatttttct tgtcatccca attatcccaa
     2041 ccagttgggt gaacatgccc acctatctta cacgcttcaa ataagacatt tgcatacgga
     2101 cgccacggtc ttcctaaata gacgtttcta gtatcaggtt ctgcagtcaa ggtacagctt
     2161 tcaaaacgat accctgtttt ttgatttttc ggcgtagatg ctgctgtgat gtatccagga
     2221 tttttactgg ctaagctatg gatatgacac tgatgaaaaa cggcatctgc cccaccaaat
     2281 atgaaatcca ccgttccttc aatccagcaa tattcatagg tctgacgaca atactcagga
     2341 ttggcagaag ccggtgtaca aaaaggagta ccatcttttt gcaaatcagg caacggaccc
     2401 gtgcataagg tatcctgata accagatagt cgacagtttt ttaaagatac gcgatctcct
     2461 gaattgaata aagcaacagc ctgtcctacc ttttctcctg gacctgctgt attttcgatg
     2521 tgaagatttt ctaatacgac attcgatcct tctaagaaaa aagtagcact tcggaaagtc
     2581 cctaactggc taccgtcaag atactcctgt tttgcaaaac gattaccgga gataactact
     2641 gttcctgctc ccacaagttt aaaattggat aatctgcttt ctatatattc atcatatctt
     2701 ccttctttta ctatcaagac ctttggatgg ctatccggca ggcctgccaa ataatcgatt
     2761 ccttcttgga tagtttcaaa atccgtcggg gtatctatag cattctttcc aattatcagt
     2821 ttgtctttca tcctcataaa tgctccctta tgaattgttt ccaaatattt ctcatttctt
     2881 gtgtttccat atgaccgcct gttaaagaaa aagaagaaaa gttttctgga ctacccattg
     2941 attgatactt tttgtccagc ccttttctca gcttcagtac gccttcgtcc aagcacatcg
     3001 tatcgttttt tccaactaag ctaagacgag ggcgcggagc gattttttct tggattgcta
     3061 acgttgtata agctgtcaat agatttggca catagtaata aaacccatga tgatctaggc
     3121 ggcgatgtag caaaagtgtt tcgatgtcca cctgtgccgc tagatcaata caaactttga
     3181 cacgagaatc caatgctgtc acccaccagc ttaataagcc tcccatagaa agaccaatcg
     3241 ttgcgagccg ttgagtatct gtgtcttccc ttgtctccaa ataatcgatc aagctgatca
     3301 catcataaat acgcattccc cataacgttt ttcctgttaa taaaaattgt ttgaacaact
     3361 cgctttcaga aatacctccg cgttcttcaa atccccaagc atcaatcgac caaacagcat
     3421 atcccattcc tgtcagtact tctgcaaaag atggagaagc taagtaagga gcaccatgga
     3481 tcaattcact tttcccgact gaaaaatctc cgccgtgaga atgcaggtaa aggaccgtcg
     3541 gaaacggtcc ttctttttct ggataagcat agagggctgg tactgtttcc aattcattta
     3601 agtacaattg atagtattca agcataaaac cgttttgttt ttctcggtat aatcgttttc
     3661 cactaggggt atgtctgcta gatggagcac ctaaaaaatc attcaactgc ataagcaaag
     3721 tttttacgaa caaccagttc atccatattt tctgattgat ttgattggaa tttctcaata
     3781 ctgatttctt tagtattttc taatagaaat gctggtccct cgtgattttc aatcgacatc
     3841 tggttaaagg aaaccttacc cgcaaagcca atataaaatc cttgtctgct catttcctct
     3901 attcctgtca tcatagcagg aacaccggga actgcatctt gggccatcga gacatcgata
     3961 ttgtcaaatg tgatttcttg tatgaattgt tccgctaatc cataaataaa acctgcagaa
     4021 gcatggacat tccttgccgt gatattcgaa aaatggatcc ttctgaatgc tgatgtccgt
     4081 tcatctatcg gataagcctt tttttcccat acatacggtt ctttccctcg cggaccacag
     4141 aaataataaa gattcagtat aaatggacac atcacattat ccatgactat attggatact
     4201 cgaatatctt caacgatccc gcctcgacct cgtctagatt tcaatcgtat tcctcgatca
     4261 gtttcttgga agatacaatt tgaaatggta atattacgaa tactaccact catttcactt
     4321 cctaatacga caccgccatg tccatggacc atcgtgcaat ttgtgatcgt gatattttcg
     4381 caagcgatcc tttcgtacgt atcttcagtc cctgccttga ttgcaataca gtcatctccc
     4441 acgtcaatat ggcaattact aatacgcaca tttttgcatg attctggatc aattccgtct
     4501 gtatttggtg aatccgctgg gttcaagatc gtcaagttat caaacgttgc attactgcaa
     4561 agaatcgggt tgatcgtcca acttggtgac tggatcaatt taatatcttt cacagtaatt
     4621 cgatgacagt tatggaaact catcaattta ggacgaggat aagcgagatt atctggttca
     4681 tttctaaaag tatgccacca tttttttcca tttccatcta atgtaccgaa accagtgaca
     4741 gagatgttct ctacattttg agcatagata caggaagcat aaacttttcg atggactcct
     4801 tcccaacgtg aatgaacaac aggataatct tttggatcgt cagagaattt cagaaccgct
     4861 cctgcgctta aatgaagttc gacattgctt tttaaaaata aagctcctgt tagaaattca
     4921 cctgctggaa caactacggt cccgcctccg tcacttgctg cagcatcaat ggcttgttgt
     4981 attgcttcag tattcaattc atcaattgat gcgccaaatg tcaaaatatc atacatattt
     5041 ataatgcctc cacttcaatc aatgcttgca agaatgcacc aacgcctttt tgatcattac
     5101 aaataattgg ttcactgatg tagtaagcat atgaaccatc tcgctgatct gctccgccta
     5161 accctgctac ttgacagttt ttattcaagt tcagccagcc ttcttttgtt tccagaacaa
     5221 attcatcaag taatccttga tgtgatttct tcacttgtgg aagccattct tctttgttca
     5281 gcagtcccaa acgtattcct ttagccattg cgtaaacaaa catactactg ccggaagctt
     5341 ccagataatt tcctttccgt tttgtttctg ttgttacttg ataccatgtc ccgctgtttt
     5401 cttcttgata tggaagtaat gcagccagtg tcttttctag aatttctttc agcaaagaac
     5461 aatctgtttc ttcatgaagt aattcatagg tatctgctgc agccatcaaa taccagccaa
     5521 tcgatcggct ccaaaaatga tgggatagac ctgtttttgg atcagcccat ggttggacct
     5581 tcttttcatc ccatgcatga atcagcaaat ttgtttttgg atcgaataga tgtttataac
     5641 tgatttcaaa ctgtctcaaa acatcttggt aatctttttc ttcactaaaa ttctggataa
     5701 attcagcata aaaaggagaa cccatatata agccatccaa ccaaatttga taaggataaa
     5761 tttctttatg ccaaaaagct ccttcggatg tgcgtggatg gtttgccaac tgctgggaaa
     5821 ggagttctgc tgctttttta tatttttctt cgcctgtttc tttgtacaat aaaaagaaaa
     5881 gctttcctgt atttacatgg tcgatattgt attcttcaac gctgtagcca cggatcgttc
     5941 catcttcttg cacgaaatag tcgatattgt cttttatgaa atcaaaataa attttttctt
     6001 gggttcgttc ccaaagtaat tggaaccctt taagtacgac gccatagtca taagaccatt
     6061 ttcctttata ccacttttct tcatataagc gaggtgttct ttccatgata ctttttgcta
     6121 atttctttga ccagttttcc attatttgct ctcctttaaa aattaaaata ggatttggga
     6181 caaataccaa gaagtatttg tcccaatcgt ctatattact gtgccgctgc tttgtatgct
     6241 tcctcgtact cttgtgtgat tcgatcgcct ccttgtgctt tccagtcttc tacagctttt
     6301 ttgaatccat cttcgtcaag ctgtcccatg atgtattgca ccgtagcatc aatgacgatt
     6361 ttttcaattt ctgatcctac ttcattgttc gtcggtgaat ccaatggcac agaaggatcc
     6421 attacagcaa attcttcatt ttctttgatc aattgattag agaattcttt ttcagggtct
     6481 gtatctttga aagagaacgt cacttcacta ggacgagagc ttgataacgg ttgcacctct
     6541 tgttgccaaa gatccatatc gataaattcg atagaaccat catcattcaa ttcataatgc
     6601 gttccttcga taccgccagt catcaattta tacacatctt cttctaaaag atcatcaaca
     6661 aattgaagta atttcttcaa ttgtgcttcg tcttttactt gagatttggg aaatgctagc
     6721 aagccgccaa taccatttcc ttctgaccaa acatgataac cgccgccttc accatctgag
     6781 atcttgttta caggcactaa ttccatatca tcttgaatac cttgacccat cgtcttcaga
     6841 ttcttgatat ccaccattcc agtgtagatt ccagttcttc cttgtgcaaa ttgttcttgc
     6901 tgatcagtct tttgggtaac tgcaaagtct tgtgccaaat agccattctc aaatagatct
     6961 cttgaaaaat tcaaagcttt catatactct tctgtatcaa attctggagt gaatttgcca
     7021 ttatcgtcta cagcccagcc atttggtgct ccaaaataag aagtgaaaag tttaaagctg
     7081 gtataacgaa tatcactagg cgcacgatcg ccaaaaccaa ctgtgtcgtc cacaccattt
     7141 ccatcaggat cgttttctgt aaattgttta gctacttcat aaagctcatc catggtagtt
     7201 ggaacatcta gtcctagatt atccaaccag tcttttcgaa tgactaagcc agcacgagcg
     7261 tagtcttttt gtatcggtac gccgtacaag gcaccgtcga tactcgcagc ttcgattcta
     7321 tcttcagaaa tttgacttaa atttttatag tcttttaaat attctgatac atcccagaat
     7381 acgcctgatt ttaacgcatt gcggacagaa gagtttttca acatcgcaat ttgaagtgta
     7441 acgatatctc ctagttcatc agaagccaat gcagttgtta atctttcttc ttttgaagca
     7501 tcaggaatcc agttgaatgt cagttccgta tcggtttttt cttcgatcaa gttcacaatc
     7561 gtgtctgtag gcggtgaagc atgatgcaaa atatttaacc aagtgatttt agcttttcca
     7621 tcttctgtct ctgaagaact gttacttgaa ctgctgccgc atgcggcaag tgctagaatt
     7681 cctgcaaaac taagacctaa ttttacgagc gttgcttttt tcataaattt tctccttcac
     7741 tgttttaata atatttttgt tttcttctgt tttttctgta tatgagttct tccattccaa
     7801 atggcaaaat atagaatttg ctgtccacgt gactgcggaa aagaagagac tgccaccgat
     7861 aaaggttaaa acacctggca ttgtttggat gatcatcaga taaatcccta ccatacttaa
     7921 tatcaacact aatgagtaat gaagatgtga gaatccgaat aaaaatgcca ttttcatttt
     7981 gaaaaaatat cctttccaat cataatgagc catgattggg aaaatataga gtgctgagag
     8041 cacatatatg aacgcagcaa tatacaaagc gatcctaaga taccaattgt gtatgaaaga
     8101 aagatccaca agtaaaacaa tcccgatggc gccatatatc cagctcatca gcaaggtttc
     8161 acgatagttt tctttaaaat atgtgaagta cgttttcgtc aaaggaaacg tttcttttga
     8221 gcgcagccat tttctaatca ctgcaaccat cgcatacgat gcaggaccga aagtaaaaac
     8281 tcccaaacct gcgatgacta aaccgcccca cagaaaattc aaataaacca actgtaaaat
     8341 acgatttagc cttccgttta ttttatctaa tgactgcagc atatctttcc ctctctttct
     8401 atcttagtaa acgccgtctt ctcctagctt tttggcaatc ttattagcta aaattaccat
     8461 aaacaatcct acgacccctt taaacaatcc cattgctgta ctgaaactta actgtccatt
     8521 cttcaatcca gctgtataaa tataggtgtc gaaaatttct gcaacgcttc gattcaatga
     8581 attcaataac aagtacatat gctcgaaccc taagtctaat gtctgaccga tttttagaat
     8641 cagcaaggta atgattactg gacgaattgc cggcaatgta acatgccatg ttttgcgtaa
     8701 gcgtcctgct ccatccattt ctgcagcttc atacaactgt gtatcgactg cagttatcgc
     8761 tgaaaggtag atgatcgtag accagcccaa ctctttccag atcacttgtc cgatatatac
     8821 tgtgcgtgtc cattcagggg atgttagaaa actgattttt tcaaatccca ttgttgcaag
     8881 gaattcattc aatacccctc cgtcaacatt caaaaataca taagtaatcg aaacaatgat
     8941 tacccaagac atgaagtgag ggatgtaaat gatcgtttga atcccgtttt tcaatcgttt
     9001 gttcgttact tcatttaata gtaaagacaa aatgattggc atcggaaaaa acacgaagat
     9061 atttaatccg aacaagatca acgtatttct taataatgta aagaatgtcg gttctgtaaa
     9121 caaacgaatg aaatgtttga aaccgaccca aggacttccc attattccta aaaatggttg
     9181 ataatcttga aaagcaataa cgagtccccc cataggaaga tacctaaaaa tcaagaaata
     9241 aaggactccc ggcagaatca tcacatataa aaatttattc gttttgattc ctgcaattat
     9301 tttttttcgt cttctgagct tttcttcttt tttcgatagc gtcagttgaa atggttcttg
     9361 tttctttaca ctttccataa gcccctcctt agttttgtga atgtttgctg cttatgactt
     9421 catgatagat aactatctga attacgtata taatcatttt tatcgtaagt aaaaacgctt
     9481 acatagcaat gatttttgtg ctatacaaaa cgataaagtg tattttttca caaataaact
     9541 acgcatagca aaccttctat ttttttctat ctgacactta gcataggaaa tgattatata
     9601 cgtaatcttt caccgatgat aaattgaaca tatagaaaca gaaacattta atttattttg
     9661 aggaggagcc tatctatatg aaaaagaaag tctctatagg agaaagaagt tttacacttt
     9721 tcaacaatac gtttctaatt ttgctagctt tgatctgtat cgttcctttt ttaaatatca
     9781 tcgctacatc ttttgcttct acacaagaag tcgtcgcgaa aaagtttatc ctttttccta
     9841 ctaccttttc tttagacgcg tatcgctaca ttctttctac gccgaccatt tttagagcac
     9901 ttgctgtttc gattggtgtt acaggtgtcg gaacgatcgt cagtatgtgt gcaacttcac
     9961 tgatggccta cggtctttcc agaaagtatt tgtttggtcg aggtttcgtc aactttcttg
    10021 ttgtattttc tatgttgttc agcgggggga tgattccaac gttcttagtt gttcgttctc
    10081 tagggctggt caattcttac tgggccatga tcctgcctgt ggctgttaat gcaatgaata
    10141 tgatcattat gagaaacttt ttccaagcct tgccggatag tttggaagaa tcagcaaaaa
    10201 tggatggttg cacagatttt ggtgtatttt tcaaaattat gctgccatta gctttaccgt
    10261 ctattgctac tatctcgctt ttttatgctg ttacttactg gaacacttat atgactgcta
    10321 ttttgtatat caacgattca tctaaatggc cgatacagat tcttttacgc caaatcgtca
    10381 ttgtatcaag tgggatgcaa gcagaaagta gtgccgttga tgtgattcca ccagcacaaa
    10441 cagttaaaat ggctgttatc gttattgcaa cagtgcctat gctgattgtt tatccttttg
    10501 ttcagaaata ttttgtcaaa ggagcactcg taggttctgt caaagggtga ttatgtactg
    10561 aacgcaaaat ggtagttgtg tcagaagtgg tcagcttcaa gaaataagaa ggcatttacg
    10621 aaaattgctt cttaaatttt tgtgaaattt cggcttattt ttcgaagaat ctactcctgg
    10681 aacaccgtgt atcagaggtt gtgaaagaga cgttcagcct tgagcgatga taaaaactct
    10741 cacaaacatt gcgacgagta acccacagga gcaataagaa gcgattttga aaaatagctt
    10801 cccatatttc acaaaattat gccttattgt cgatagcctg tctcttgaac gccatttatt
    10861 cgtaaaaaag aactgtgaca agacttttgt cacagttctt tttgcgtgct tagccttgaa
    10921 atttaactat ccataaatac aaatactagg gttattctaa cttcttttat tgcttgaatc
    10981 agtccacaag aaaaacagcc tcttgattaa acggcgttta tcttgttgat tctgcggcaa
    11041 taaaatcagc cagcagcaaa acatgaagtg ctgttttttg ctgtcctctt ttattgcttg
    11101 aatcagatca caagaaaaac agcctcttga ttcacaaata tttttttcga tattctcccg
    11161 gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt ttgcggattt cgatattgca
    11221 atctttctgc aatctctttt acggagaggt tagtctcttt caaccatttt tttgctacct
    11281 ctaaacgatg attttgaaca aaatcaccaa atttttctcc tgtttccttt ttaaatacat
    11341 tacttaaata gttagggtta taatgcaacc tatcagcgat cacatctaaa gacagatcac
    11401 gatcatattc tgtgtagacg atatgcataa tattctccga caaattttta aattcatgat
    11461 cagtgattgc ttgagagcat tctgtgattg gcagaactaa ttgttctaaa acaagttttt
    11521 ctagttcttt gggatgataa ttgttcagtg cttttacata aatttgtttc atgttatcaa
    11581 agttttttga atctactccc aataactgtc ctaattgaac gagttcattg atcaagcgga
    11641 ccgaaactac ttcccgactc aaaggatttt tattttgtgc aaacaatgca tcaatcaatt
    11701 catgtactaa attcttcact tcctgatttc cagaacgaat ggcttcaaac aatcgatttt
    11761 gttcttctat cggatatcta ataagtgttt tttcatgagc agcaggaacg atttcctgat
    11821 aaaaaataat tgaacttgga ccagtattta cacgataata cagggcttct tttgcccgat
    11881 caaccgattg cttgctttca ttcagcgttt cgaaccggtt actgatgcct atactgatag
    11941 ttaaattcaa gtattctttt acacgttttt gaatgagtcg gcaatactcc atgacttttt
    12001 tgcttgcttc tgcatcatct ttatccattc gataaatagt ggcttgcatt tcttcattca
    12061 aaacgatcgg cagcattcga ttttcctttg gcacaatttc cgtgatcatg ttgttgatcg
    12121 ctaaaagtag caaatcacgc tccccaccgt gactatcttc taacagatcg atttgaacca
    12181 aagctgtata atagcagtca ttttgagaac tataaccaaa ttgctgcaac cgctgatcta
    12241 gttctcgcct ttctactcga tttctaaata aactaagtac aaatagcgtt tccagctgtg
    12301 gcttttgggt aactaagtgt gcacttaacg cttctttttc tcctatgatc cgactgactg
    12361 attgagcgac caaatctagt tctttcccac tgaatccttt tgatttaaga ttcagacgtt
    12421 cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa taagtaggaa aaaagaccaa
    12481 tgagtcctac cagtgttaaa gacactgcaa ctagcccgat tctcagattg cggatcattg
    12541 cgccaattgc actagagtca acttctaatc tgtaaatcca attattataa tctgatttca
    12601 gtactatata cgttttatct ttttctctta tcactttcag ctgattgtaa tctagatcca
    12661 cgtccgttaa tacagttggt tgctctttat tttttactgt ctgagtagta aaaaggttgc
    12721 caacttcgtt ttcaatagaa agtatgtctt ctccactttc accaacaact tgttctattg
    12781 tatgtttctc tatcgtagca attcccaatg catatttttc tttttcaaac attggcaaag
    12841 aaatcaccat ttcgattcct ttgtttttct tttgccaaaa taaattattt ttgttattta
    12901 tgtacttctg ttgatacttt tcaacttcct cttcggtcag ttgactcaac gatccattga
    12961 ttactcccca tttaccagcc aaactgacta aatcataatt attatcatcc ataacaatcg
    13021 tttctataaa atttagttct ttcctgattt cagaataagt ggagtaatct gtatatgtca
    13081 gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc atacgtagaa aacgaatatt
    13141 caatcgttct aattttccat tcaacgttag ttttaacttg cctgagcaaa ttctttttgt
    13201 ctgttaaata actcgattcg atcgattcat aggtattctg gtaaatgaat aaactaaatc
    13261 ctatgacaag tacggctcct aataaaaaca tcggtaaaaa tattttataa aatactgtac
    13321 ttttcttcat tttcttcctc tcctcctttt aaaataaaag gctttcattt ttctgtaaaa
    13381 ttgatactca tctaattaaa tgagcattcc ccgctttcaa gaatagcata attaaaaata
    13441 aaaatccaag taatttttaa cgtgtgcaat cttttcatat caaagatcta ttgacttatc
    13501 gtagttatcg taatatgatg aatgcgttaa caaaaagtga aaaagtaagg aaaatgatgg
    13561 aatggtaacg atcaaagata tcgctaaagc agctggcgtt tctcatacga cagtttctcg
    13621 tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa caaaaaatac gacaactagc
    13681 taaggaaatg aattattcgc caaactatag tgcaaaaggt ttagtgaatc aaaaaaaata
    13741 tctaatcgga ctatttttct caagtttgca taaaggaact tcatcaaatt tcctagcaga
    13801 ttccattgca ggcatccatt cagtattaga cacaaattac agtttatctg ttgaaagtat
    13861 cgatgttgtt gatttaacag aaatcaacct gcaacgctac gatggactga tcgttatgag
    13921 tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa gaaaaagaat ttcctttcgt
    13981 tgttgtcaat cgtcacatca atgatccagc ggttatcaac gtcactgccg atgatgcggc
    14041 cggtgttatg caggcagtag attacggtat tggatgcgga catacgaaga tcgcttatat
    14101 tggcggaaag aaaaattttc gttcgacaaa agaacgggaa aaaggggtat tgaaaagtct
    14161 aaaaaaagcg agcatccctg tcaatctagc ctatttttta acaggagatt acagcttaga
    14221 aagcggcctc caaaacatgg aatacctatt atcactttct gacctgccta ctctggtttt
    14281 ttgcggcaac gacgacatag cgattggtgc attgagagct gtacatttag ctggcctccg
    14341 tgttccccag aacatttctc tgatcgggtt cgatgattct ccagtcgtct cctatctcaa
    14401 tccgccgctt accaccgtac gtaagccttt gaaagaaatg tgtcaagaag gaacaaagct
    14461 tttactagat ttgatcgaag gcaggacgat cgaacaatct aaaattgagc tctccacctt
    14521 gttgatcctt cgtgagtcga tcaattactt gtaaactaca agtaatttta tataaaaatg
    14581 ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa aactgacaaa agaattagtg
    14641 aaaaaaaacg aagcacctgt aaaagttctt tagtttggtg aaggcaattt tatgcgtgga
    14701 tttattgatt ggcagattca gcagctgaat aaccaacaat tgtttaaagg taatgttgca
    14761 atcattcaac ctttagaaca aggtcttgga aatatgatga accagcaaga taatttatat
    14821 acggtcattt tacaaggact cagcaatggc gaaatcgttg atacttccga gattatcacc
    14881 gtagtagaac aaatgatcaa tccttatgat gaatgggatg cttatctatc tctagcagaa
    14941 aatgacgact tggcctatat cgtctccaat acaacagagg caggaatcca attcgtggag
    15001 actgataatt taacagatac tcccccacag agcttcccgg gcaaactgac agccttttta
    15061 taccgacgtt tcaaattaaa caaagctggc tttaccatca ttccttgcga actgattgac
    15121 agaaacggcg aacgtctaaa agaaatcatt ttacgctacg cagaaaaatg ggatttagaa
    15181 cctgcttttg ttgagtggtt agaaaaagaa aatgttttct gttgtagttt agttgatcgc
    15241 atcgtcccag gttatcctcg cgaagatgca ggtgttttca atcaacggca taactatgaa
    15301 gatcaattga tggtcaaagc agaaccattc atgctttggg tgatcgaagg gcctaaagaa
    15361 ttggagaatc aactaccatt caaacaggct ggcttgaatg tcatcatcac agatgatatg
    15421 actccttatc gtgaacgtaa ggtgcatttg cttaatggac cgcatacagc gatggtccct
    15481 cttgctttgt tggcggaatt ggaaactgta gaagaagtaa tgaaagatcc tttattctcc
    15541 gcatatgttg atcagttatt caatctagaa ttgattccta tgctttcttt acctaaagat
    15601 gaattagcca tttatgctga ccaaatcaaa gaacgtttct tgaatccttt tgcccatcac
    15661 aaattagaag cgatttcatt aaatagtgtt tcaaagttca gcacccgtct gcttccagtg
    15721 ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt taatcactgt ttcactagct
    15781 gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac atgatgatga aaagacgata
    15841 agcgaattta ctgatgcttg gtctgataat ggaacagcta ttcctcgatt attgcaaaac
    15901 gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg tgacagatac agttcaagaa
    15961 atagctgcaa agatcgatca aaaaggtgtc cggtcagtat tacaagaaat gaaaggaacg
    16021 atggtccatg cttgaaaccc tgcaaccaac aatgaaatta aatcgacgag acagtgtggt
    16081 cgtcgcatta acccctattc caaaacaaac acaactgacg attgatcagc aaacgatcac
    16141 cactcttgaa gatatcccgc aaggtcataa gattgcattg ggtgatttga aagctggaga
    16201 caacgtgatc aaatatggct atccaatcgg tcatgtgacg acagacgtaa ctgccggtca
    16261 atggctgcac actcataatg taaaaaccaa tctttctggt gaattggatt atcgatatga
    16321 aaaagatgtc catccaagtc attacctttt tgagaaccga acatttcaag gttacttgcg
    16381 aaaaaatgga aaagtaggga ttcgtaatga tttgttttta gtacctaccg ttgggtgtgt
    16441 gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa aaacatccag atctgggaca
    16501 gtttgaccat attacgattt taaagcatcc ttatggctgt tcgcagcttg gaaaagatca
    16561 tcaaaatact cgagaaattt tagcggacgc agtaaatcat cccaatgccg gaggagtttt
    16621 agtatttgga ttaggttgtg agaacaatac agttcctgaa ttcaaaaaaa ttttaggtga
    16681 atatgatgaa gaacgagtga aatttctagt ggctcaagat gtttacgatg agatcgaaca
    16741 aggtgtggct ctattagaag agcttttaac agcggcggaa aatgaccagc gcatatcagt
    16801 cccgctctct aagttgaatg tcggtttgaa atgcggcggt tctgatggtc tatcgggaat
    16861 aactgctaat cctttacttg gtgcattttc tgactacttg attgctcaag gaggcagcac
    16921 gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa gtattgatgg cacgagcgga
    16981 aaatgaagaa gtttttgact caatcgttga cttgatcaat gacttcaaac agtatttctt
    17041 gtcttacggg gaaccagttt acgaaaaccc atctcctggt aataaagcag gtgggatcac
    17101 tacattagaa gataagtctt taggctgcac gcaaaaatca ggaagctctc ctgtcgttga
    17161 tgtgctacaa tatggcgaaa aaatcagaaa accagggctt agtttattgc aagctcccgg
    17221 gaatgatctt gtagctgcct ctgctcttgc ttcatccgat tgccagttgg tcttgtttac
    17281 cacaggaaga gggactccgt ttggctcata tgtcccaacg gtcaaggtat ctacgaatac
    17341 aactcttttt gaacgcaaag gtcattggat ggattttaat gcaggcgtct tattagaaca
    17401 accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc atcgctgtag ctagtggaga
    17461 agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc atttttaaaa acggagtaac
    17521 actttgattt tcataaaaca gtataaaaag aaagaaggaa gatttatgtt tttatctgat
    17581 gattttttat taaaagacga ttgggcgaaa aagctttacc attcctacgc aaaaaacatg
    17641 cccattattg attatcactg tcacttagat ccaaaagaaa tttacgaaaa caacaatttc
    17701 agcaatctga cagaagcttg gttaggaggc gatcattaca aatggcgctt gatgcgagct
    17761 tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg attttgaaaa atttttagct
    17821 tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt acagttggtc acatcttgaa
    17881 ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag aaaatgcaga gaaaatttgg
    17941 acacaagcaa atgaaatttt ggcagctcct gattttcgcc gtcgggaaat cgcaaaaaaa
    18001 tcgaatgtag aagtgatttg tacaacagat gatcccgcag acgacttgca ctatcatcag
    18061 ttgcttgctc atgaagaacc agatttgaag gttctacctt cattccgtcc agataaagca
    18121 ctcaatattg aaaaagaagg atttaatgca tgggtcacac tactggaaga agcagctgac
    18181 caaacgatca ccacctatca agaactgata gaagtcttag ggcagcggat agaatacttc
    18241 catcaacatg gatgtcgtgt ttccgaccac gctttagaca tcttacgcta taaagaagcg
    18301 gatgaaacag tattagaaga gatttttcaa aaagcacgag ctaacaaact attatcttca
    18361 gatgaaatcg atgcttatct tacagaaaca ctgatacatt tgacccactt ctatcattct
    18421 cataattgga cgatgcagct gcacatccat gcttatcgta actgtaatac acaaatgttg
    18481 gaaaaattag ggcccgatac cggctatgat ggaatgaatg atctttcttt gactattcct
    18541 ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt tgccaaagac gatcctttat
    18601 tcattgaatc cgaatgacta tcctgcgatt ttagctttga tgggctgttt ccaaaaagaa
    18661 cagaatggaa aactgcagtt aggttctgga tggtggtaca acgatactcg agctggtatg
    18721 cgcgatcaat tgacacaatt tgctgatggt agtgctttgg ggaattttgt cggcatgctg
    18781 acagattcaa gaagtttcct gtcctacact agacatgaat atttccgtcg tgttctttgt
    18841 gaatttttag gcgaaatggt catgcgagga gaagcacctg aagatgaaca acttttagga
    18901 agtctagttc aagcgattag ttatacaaat gctaaaaatt actttggctt ttttgcctaa
    18961 ttgattaata gtaacagtaa cgaatcaaaa acaggatagt gaaataacgg aatccgttta
    19021 tccacgtttt agagaagaag acggaccaaa tt
//
LOCUS       DAXLPI010000049        18539 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXLPI010000049 DAXLPI010000000
VERSION     DAXLPI010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.049"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            144..791
                     /locus_tag="ACF3T5_001481"
     CDS             144..791
                     /locus_tag="ACF3T5_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFD3632093.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    920..1136
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1184..3829
                     /locus_tag="ACF3T5_001482"
     CDS             1184..3829
                     /locus_tag="ACF3T5_001482"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFD3632094.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4139..5452
                     /locus_tag="ACF3T5_001483"
     CDS             4139..5452
                     /locus_tag="ACF3T5_001483"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFD3632095.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5442..6095
                     /locus_tag="ACF3T5_001484"
     CDS             5442..6095
                     /locus_tag="ACF3T5_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3632096.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6150..6842
                     /gene="radC"
                     /locus_tag="ACF3T5_001485"
     CDS             6150..6842
                     /gene="radC"
                     /locus_tag="ACF3T5_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFD3632097.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6992..7192
                     /locus_tag="ACF3T5_001486"
     CDS             6992..7192
                     /locus_tag="ACF3T5_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD3632098.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7446..9125)
                     /locus_tag="ACF3T5_001487"
     CDS             complement(7446..9125)
                     /locus_tag="ACF3T5_001487"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFD3632099.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9127..9339)
                     /locus_tag="ACF3T5_001488"
     CDS             complement(9127..9339)
                     /locus_tag="ACF3T5_001488"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFD3632100.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9732..11462
                     /gene="efrA"
                     /locus_tag="ACF3T5_001489"
     CDS             9732..11462
                     /gene="efrA"
                     /locus_tag="ACF3T5_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFD3632101.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11459..13219
                     /gene="efrB"
                     /locus_tag="ACF3T5_001490"
     CDS             11459..13219
                     /gene="efrB"
                     /locus_tag="ACF3T5_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFD3632102.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13314..13511
                     /locus_tag="ACF3T5_001491"
     CDS             13314..13511
                     /locus_tag="ACF3T5_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632103.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13664..14149
                     /locus_tag="ACF3T5_001492"
     CDS             13664..14149
                     /locus_tag="ACF3T5_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFD3632104.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14253..14846)
                     /gene="clpP"
                     /locus_tag="ACF3T5_001493"
     CDS             complement(14253..14846)
                     /gene="clpP"
                     /locus_tag="ACF3T5_001493"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFD3632105.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            15026..15547
                     /locus_tag="ACF3T5_001494"
     CDS             15026..15547
                     /locus_tag="ACF3T5_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632106.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDV"
     gene            15644..16381
                     /locus_tag="ACF3T5_001495"
     CDS             15644..16381
                     /locus_tag="ACF3T5_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFD3632107.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16385..17302
                     /gene="miaA"
                     /locus_tag="ACF3T5_001496"
     CDS             16385..17302
                     /gene="miaA"
                     /locus_tag="ACF3T5_001496"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFD3632108.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17304..18533
                     /gene="hflX"
                     /locus_tag="ACF3T5_001497"
     CDS             17304..18533
                     /gene="hflX"
                     /locus_tag="ACF3T5_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFD3632109.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVNRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEIAVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5992 a         3209 c         4031 g         5307 t
ORIGIN      
        1 tggttgagct tattaccgca gatgcaagct tttgaacacc gttttatttg tctcacaccc
       61 ttttccttcc tacttgtaac cttgctcccg tgttagaaac atgttacact aaatatgtga
      121 aattattaac aaggagcgtc gttatgcgag aaaaaacaga tacactgcca aaagcaactg
      181 cgaaaagatt acctttatat ttacgctact tgaagatgct ggatgactca ggtataagta
      241 gaatcaaatc aaatgagttc agcgaaatca cccaaattcc ttctgcaacg atccgcagag
      301 acttctcgca attaggcgag ctaggcagaa gcggatatgg ctatgatgtc ccttttctta
      361 tcgatgtatt caataatatc cttaatacta aagaagaaaa acgaatcgca ttagtaggat
      421 atggaaatct tggcaaagca ttgaaacaca acaattttag aagaaatgaa aatctaaata
      481 tcgtgtgcgt ctttgataat gatccagcac tgatcaatcg agtaattgat ggagaaatga
      541 tctatccgat tgatcgtttc gcggagatag ccaaagcaaa aaatgtgaca gtagcaatct
      601 ctacggtacc aagtaaatat tcacaatctg ctattgatga aattgtcaaa ggaaatgtaa
      661 ccgctatact taattttgcg cctgacagag taactgtccc agcttatgtc aatgttcaat
      721 acattgattt gacaaccgag ttgcagacgc ttatttactt tgatgagaat tactcagaag
      781 ttttttctta ggaaaatttg agtaggctta ttgatcttgc gagaactcgt cgtaattctt
      841 tcaaaaattt tttagtacta aatgaagtgg ttttttcacg accacttgtt gactaaaaaa
      901 aaaaaccatt gtagaatgaa gcataacgct gaacaagagg agtaactggc acattcattc
      961 ttagagagag aactatctgc tgaaaggttc tcaatgacaa ccagtgaagg tagcttggga
     1021 gtttctgtct tgaatggagt agagatagac gagtcgtgat cgttaactca cttgagaggc
     1081 aataatgatt gattattgca atttaggtgg taccgcgtaa atcatacgtc ctagaatacg
     1141 aaagtatttt aggacgtttt ttagtttaaa ggaggaaaac agcatgacgg aagaaaataa
     1201 cctatcaaca aaatataatc cgcaagaagt tgaagctgga cgttatcagc attggttaga
     1261 acaagatttg tttaaaccga gcggagataa aaaagcaaaa ccttattcaa tcgtaatccc
     1321 gcccccaaat gttactggaa aacttcattt agggcatgct tgggatacga ccttgcaaga
     1381 tatgctgatt cgtcaaaaga gaatgcaagg atatgatact ttatggctgc ctggtatgga
     1441 ccatgcagga atcgcgacac aagcaaaagt agaagcgaag cttagagagc aagggatttc
     1501 tcgttatgat ctaggtcgtg aaaagttcat cgatcaagtg tgggaatgga aagaagagta
     1561 tgcgagccac atccgcgaac agtgggctaa aatgggctta tctttagatt acagtaggga
     1621 acgttttaca ttagatgatg gattatctga agcagtacgt aaagtttttg ttaccttata
     1681 cgaaaaaggt ttgatttatc gtggtgaata tatcatcaac tgggatcctc aagcacgaac
     1741 agctctatct gatatcgaag ttattcataa agatatcgaa ggcgcctttt atcatatgag
     1801 ctatgaacta gcagatggat caggcgtagt agaaattgcg acaacccgtc cagaaacgat
     1861 gttgggagat acggcgattg ctgttcatcc agaagacgag cgttatcaag cgctcatcgg
     1921 caaaaaagtg attctacctt tagtcaataa acaaatccca atcattgcag atgaatatgt
     1981 agatatggaa ttcgggacag gtgtagtaaa aatcactcca gcacatgatc caaacgactt
     2041 tgaggtaggt aatcgtcatg atcttccacg cgtaaatgtt atggatgatg atggtacgat
     2101 gaatgaacta gcaggaaaat atgctggaat ggaacgtttt gctgctcgta aagcaatcgt
     2161 gcaagatctg aaagaaatcg gtcgattgat taaaatcgat acgatgatcc acagtgtggg
     2221 tcattccgag cggacaggtg tcgttgttga acctcggctg tctactcaat ggtttgtaaa
     2281 aatggcgccg ttagcgaaaa aagccattga taaccaagaa acagatcaag cagtggaatt
     2341 cttccctcca agattcaatc aaacgttttt acgttggatg gaaaatgtcc atgattgggt
     2401 aatctcacga caattatggt ggggacatca aattcctgca tggtatcaca aagaaacagg
     2461 tgaaatgtat gtagggatgg aagcaccagc ggatagtgaa aactgggtac aagatcctga
     2521 tgttttagac acttggttta gttcagcttt gtggcctttc tcaacaatgg gctggccaga
     2581 tacggaaagc gaagattata agcgatactt ccctactagc actttagtaa ccggttatga
     2641 catcattttc ttctgggtaa gcagaatgat tttccaaagc ttggaattta ctggcgaacg
     2701 tccatttgag aatgtcttga ttcatggatt gattcgtgac gaacaagggc ggaagatgag
     2761 taaatcatta ggtaatggaa tcgatccgat ggaagtaatc gaaaagtatg gagcagatgc
     2821 tctccgctgg ttcttatcga atggttctgc accaggacaa gatgtccgct tcagctatga
     2881 aaaaatggat gcttcttgga actttatcaa caaaatctgg aatgcttcac gtttcgttat
     2941 catgaatatc gaaggaatga ccgtagaaga gatcgattta tctggagaaa aatcagtggc
     3001 tgaccggtgg atccttactc gtttgaatga aacgatcgaa cgagtaactg agctatttga
     3061 tcgttttgaa tttggtgaag caggtcgaca actgtataac tttatctggg atgatttttg
     3121 tgactggtac atcgaaatga gtaaagaagt actttatgga gatgatgctg tttcaaaaca
     3181 aaccacccgt agcatcttag tccatacact tgatcagatc ctacgtttat tgcatccaat
     3241 catgccattc gttacagaag aaatatggga acatattcct catcagggaa attctctagt
     3301 agtggcagaa tatcctgttg tacgtccaga gtttgatgat gaaacagctt ctaaagggat
     3361 ggaagtattg aaagaattga ttcgagccgt tcgaaatatt cgttcagaag tgaatacacc
     3421 attgtctaaa ccaattacgt tgctaattaa aacaaacgat gctaaaatcg atcaattttt
     3481 agttgaaaat acaagctata tcgaacgctt ctgtaatccc gaagagttga cgatatcaag
     3541 tgagatcact gcgccagatc tagcgatgtc agctgttctg acaggtgcag aaatcttctt
     3601 gccgttagca ggattgatca atattgaaga agaaatcaaa cgattagaaa aagaactgga
     3661 aaagtggaca agcgaagtaa aacgtgtcca aggaaaactt tctaatgaac gttttgtttc
     3721 taacgcgccg gaagaagtag tagaggcaga gcgtgcaaaa gaaaaagatt atttagagaa
     3781 gcaaacggct gtcaaagaac gaatcgaaag tttgcgtact attcaataag aatagaaaaa
     3841 gagagtgtga aatcagctag ctattactaa aagctagctg attgaacatc gttacaggaa
     3901 agagtttgag cctcactctt agcttcgatt cataaggaga tgtgacataa attttgtcgc
     3961 atctcttttt tagtctactc ttattatgta ggagaaggag ggaggtggat catgtatcaa
     4021 gagtcaataa agtagttgat aaaatcgttc ctctctctgt ttcttttctc tttgtcatac
     4081 ttttattttt cttatgtgtt ctatttgtta taataaaaga gaaatggggg aattgttgat
     4141 gacgattgaa gaagcaatag aatggatcca tagtcgatta ccatttggtt cacgtcctgg
     4201 ccttgaccga gtcgaagctt tattagaagt ggtagggcat ccagagagaa aagtaaaaac
     4261 gatccatatc gctggaacaa acggaaaagg ttctactgta gcttatttga gaagcctatt
     4321 agaagaggct gggctgacag taggtacatt tacttcacct tatatcgaaa cgttcaatga
     4381 acggatcgca atcaatggca aaagcattcc agacaatgat ttgatccagc ttgtaaaaaa
     4441 atatcagcca ctgatcaaac aacttgatca atttgaagca gtagctggta ttactgaatt
     4501 cgagacatta acagcaatgg catttgatta ttttttgatg aagaaagtgg acgtagcgat
     4561 tatcgaagtt ggcttaggcg gcttattgga ttctaccaat gtagctgtac caatgctgac
     4621 gggaataaca acgattggac ttgatcatac cgaaatactt ggagatacaa ttgaagaaat
     4681 cgctgcacaa aaagctggaa tcatcaaaca aggcgttcca gtagtaacag gaaatattgc
     4741 cccagaagct tttactgtca tccaacaaac agctaaagca aaaaatgcac cgctttatgt
     4801 atacggggaa aattataaaa taaactatct gcatcctgat gagatgtggg gagaagtatt
     4861 tgaatttttt aatgaaatag gaaagatccc tcaattaacc actccgcttt tagggcaaca
     4921 tcaagctgaa aatgcaggag tagccatcga attgtatttt gtttactgtc aattggaaaa
     4981 gctgcctttc cgagaaaaag atgttttgaa gggattgaag aaatctcgat ggccaggaag
     5041 aatggagcgt ttgagtaacg agccattagt tgttttagat ggtgcccata atacgcatgc
     5101 aatgaaccgg cttgttgaga atttgaaaaa agaattcaaa ggatatcaaa tcaacatttt
     5161 gttttccgcc ttacgaacta aaaatgttag agaaatgcta gaacgattgc agcaagtacc
     5221 aaatgtccat atttatttga cgacctttga acatccaaaa gccatcgaat taagtgaagt
     5281 atcagattta gcaagtgaga gagtatcaat cgtttcattg tggcaattcg gattagcaga
     5341 tatattggaa aagatgacaa atgaagacgt attagttgtg accggttcac tttatttcat
     5401 ttcagaagtt cgccatttat taatacaatt aggaggagat catgaagtat aatgcagtga
     5461 tttttgacat ggatggtttg ctgttcgaca cagagttagt gtattacgaa gccacacaag
     5521 tagtagcaga caagatgggt tttccttatg ataaagacct gtatttaaaa ttcttaggtg
     5581 tttcagatga agaagtatgg gcgaattatc atatgatttt ctcggaattt ggcaaaacaa
     5641 gtgtacaaag attcatagat gattcatacc aggaaacgct tgaacgtttt tcgcaaggag
     5701 cggtacaaat gaagcccggt gtgttggaac tcctctcttt tttagaagaa catcagattc
     5761 ccaaagttgt cgcttccagt aaccaacgag cagtcattga acttttattg aagaagaaca
     5821 atctgtttga acgatttgag acgattgttt ctgcagaaaa tgttcaacga gcaaaaccag
     5881 acccagaaat tttcttgtta gctaatcaga agctaggtac agcgaagaaa gaaacattga
     5941 ttttagaaga ttcacaaaat ggtattttag cagcttctgc ggctgagatc ccggtagtca
     6001 tggtaccaga tctgcttcca ccatcccaag aattgatcaa aaaaacagct ggggttgttt
     6061 cttctttaca tgaaatccct gcattgttaa aataaaaatc catctctttt cgtatcttat
     6121 tttagataag atagaaagga gatgtttttt tgggaaaacg attgatcaaa gaagtaccta
     6181 ctagctcttt accaagggaa cgtatggaaa tttatggcgc ggaagcttta tcggatcagg
     6241 agcttttggc aatcttactg cgtaccggtc aacaccctta tagtgtgatg tcgattgctg
     6301 gaaatctgtt aaaaacgttt ggaggactgg cctctttacg acaggctact ttgcatgagt
     6361 tagaagagat tcgagggata ggacgcgtga aagcaatcga aatcaaagcc ttgatcgaat
     6421 taggtcgacg aattcagacg gatcatgaaa gagcggctcc accggttcgt accagctacg
     6481 aattggccca gcaattgatt ctggaaatga aagatcacaa acaagaacat ttggtttgta
     6541 tttatttgga cacgaagaat caagtgatct taaaaaaaac agtgttcatc ggttcgctga
     6601 atcagtcagt cgctcatcct agagaaattt tccattatgc tgtacgttat tgtgcagcaa
     6661 ggatcgtttt agcgcataat catccttctg gtaatgttat tccatcgcag caggatatga
     6721 attttaccaa aaggatacaa aagtgtggag aaatgatggg gatcactgta ctggaccact
     6781 tgatcatcgg acgcaaacga tattttagtt tgagagaaga aggaatgatg gaagagaagt
     6841 aagttgcttt cttgcaaaaa aaatgaagca agtgtacaat tatattgtaa ttttgtttgg
     6901 gaatagaagt tgaaacatct gttgcacctc tatcgtacac gagcaatgtt acaatttaaa
     6961 ttagtgcttt tggcacgagg aggatattat tatggaaaac ggaacagtaa aatggttcaa
     7021 cgctgaaaaa ggatttggat ttatctcacg tgaagatgga agcgacgtat tcgttcactt
     7081 ctcagctatc caaggtgacg gattcaaaac attagaagaa ggtcaagcag tgactttcga
     7141 cgttgaagaa tcagaccgcg gcccacaagc tgcaaacgtt gtaaaagctt aattttatct
     7201 gtaaatcaaa ttgatggtag acatcaattt ttacgagaga acaaagcgag taacaaaaat
     7261 acctgccaca agagacaatt tctcttgtgg caggtatttt tttgtatgaa aaagtaaata
     7321 aatttaagag gctatgaaag agccaccttg tatcaaggaa taagaacaaa caaaaatggc
     7381 ttctcatgtt tttcatttgt tttctcttat attcaggaca aggtgctgat ttcataacct
     7441 cttatttatt cttcatctgg tgtcattacc attggcaaaa tcattggatg tctttctgta
     7501 cgttcgaata agaaaggctg aagcgcgcca gtcatcgctt ctttcaattt atattctgaa
     7561 cagtcaggtc cattcaaaca ttctcttaaa gcgtggaata agattcgctg agcttcattg
     7621 atcatttctc ctgattctcg catgtagaca aatccacgag agagaatatc tggaccagcc
     7681 aagatttctt ttttcttgat atccactgtc gcgacagcta atacaagacc ttcttctgaa
     7741 agcaggtgac ggtcacgtaa aacgacatta ccaatatcac cgatcccgct accatcgata
     7801 tacacatctc cagccacaaa atgcccagca ggacgcgcag aatcgctagt taacgctaaa
     7861 acatcgccat tttccatgat gaagcagttt tctgcaggaa cacctacatc ttgtgctaga
     7921 gaagcatgta ctttcaacat gcggaattct ccgtggacag gaataaaata ttttggcttc
     7981 atcaaacgaa gcattaattt ttgttcttct tgtccaccgt gaccggaagt atggatgtta
     8041 ttgatttttc cgtgaatcac ttctgcacca gcttcagaca gcaaattgat caaatgattg
     8101 acacttgttg tattaccagg aataggtgaa cttgaaaaga tcactgtatc gtctggctgg
     8161 atttggattt gacggtgagt accattcgcg atgcggctca acgcggccat cgcttctcct
     8221 tgagaacctg tacataggat catcgtttca ttggcaggca agcgattgat ttcgtgtgca
     8281 tctacaaatg tgccgtctgg aactttgata tatcctaaac gttgaccgtt gacgatggca
     8341 ttttccatgc ttcgtccgaa aacagcgatt ttacgaccag ttgccacagc ggcatctgct
     8401 gcttgctgca aacggaagat gttagaagca aagctggcaa aaatgatacg tccttcgatt
     8461 tttgaaaaga tcttcaaaat agaatgccca atcgtttttt ctgatttagt aaatgttgga
     8521 atctcagcat ttgtactatc agataaaagg cacaagactc cttcttcccc gatacgagcc
     8581 attttatgca agtttgctgg ttcgccaaca ggagtgaaat cgaatttgaa gtctccagtc
     8641 gctacgatat ttccagaagg tgttttcacc acgatcccta atgcatctgg gatactatgg
     8701 gtcgttctga aaaagctgac agaagtcttt ctaaaacgga tgatcgtatc ttcattgatc
     8761 tcatggagct ctgcatctcg aagcaagcca tgctcatcta atttgtttgt gatcaacgct
     8821 aatgcgagag gaccagcata gataggaata tttgcttgtt tcagcaagta gggtattccg
     8881 ccgatgtggt cttcgtgccc gtgagtaatc accaaggctt ttactttgtg taagttttgt
     8941 atgatgtagc tgtagtcggg aatgacatag tcaattccaa gtaaatcgtc ttccgggaat
     9001 ttgatcccgg catcaatcaa aatgatttca tcttggaatt gtaccccata cgtatttttc
     9061 ccgatttcac ctaatccacc aatagcaaag acgcctgtct cgttattttt gatggaaagt
     9121 ttcatattaa aactccacta atgagaaatc agcattttct ttttcgtact ctagatgact
     9181 tccttctaga agttgaacga attcaatatt gtatggggta ttttcttcca cacgttggcg
     9241 agcgtctaca tcgctttttg cctcaatgta aagagatttt gtttcttccc gtttagggtt
     9301 gcgtactttt gtttcttgat aataaacttt atagatcatt gttttgtctc ctttatctgc
     9361 gtaaagcagt gatttagcta gttgattttt ttagacaaca caaaacgagg ggacaagcca
     9421 cctggttttt catatgcttt gactagaata ctggattcga gtcaaagaga caagcctcgt
     9481 ttttgcttat ctgttcggat attttttcaa ccgtaccttc tgtataaaaa atatacaaca
     9541 attaattgtt attctagcat aaaacagcaa ataagtatag aaatcaacta gattgaaaga
     9601 aaaaatcaat gtttatcatc ccgctataat cataatgact tttttatgag agaagtgttc
     9661 tattaatagg ctcgttgtgc tatcattttt agtaatgaaa atgtaacaaa agattaaaag
     9721 gagatgagaa agtgaaacta atgtggcatt ataccatgcg ttataagaaa ttcttgtttt
     9781 tcaatttcat atgcgtattt ggttttattt tgattgaatt aggacttcct acgattttag
     9841 cacgtatgat cgatgtagga atccgcaatg atgattttga ttatgtgaag caacaaggga
     9901 tatttatgat cgttattact gtaatcggta ttattttgaa tatcttgtta ggttattttg
     9961 gttcgaggat tacgactaat atcgtagcag atattcgaga tgatttgttc gaaaagatcc
    10021 aaagttattc gcatcaggaa tacgaaaatc taggcgtatc ttcattgatc actcgtacaa
    10081 caaacgatgc ttatcagatc atgttgttcc tgcaaaatat tttacgtatc ggttttatgg
    10141 caccaatgat gttcattgtc agtttgtaca tggtcatgcg tacaagtgtg acactcagtt
    10201 tgtatgtagt aggtgcatta cctttactat tattggcagt cgtagcgatt gctaaggtgt
    10261 ctgaaccgtt atctaaaaag caacaaaaaa acttagataa aatcaacagt atcttgcggg
    10321 aaaatctttc aggattgcgt gtgattcgtg cattcgtcaa tgaaaagttt gaagaaaaac
    10381 gttttgctgt agtcaatgag aattatgctt ctagttcaaa aagcttattc cgtttgatgg
    10441 ctgttgcaca accgggtttt tatttcttat ttaatatcgt aatggtattg atcatctgga
    10501 gcggggcagt gcaaatcgat gcaggcgagc ttcgtgtagg agatttgatt gcgtttatcg
    10561 agtatatatt ccatgccttg ttctcattca tgttatttgc tagtgtgttc atgatgtatc
    10621 caagagcagc tgtttcagct ggacgtatcc aagaagccct agatgccaaa ccttcgattg
    10681 tagaagatcc aaatggtata tctgatacaa aaactaaagg gtatatcgaa tttcaaaatg
    10741 taacatttgc ttatccaggc cattcgcaag agccagtgat acgaaatgtc agttttacag
    10801 cctctccagg agagaccgtt gcttttattg gaagtaccgg tagcggaaaa tctacgttga
    10861 tccaattgat ccctcgcttt tatgatgtga gtgaaggaaa gatcttgatc gatggagtag
    10921 acgtgagaga ttatcggcta agcaagttgc gagataagat tgggtttatt ccgcaaaaag
    10981 cgctgctatt tactggaacg attgcagata atttgcgata cgggaaagaa gatgctacgc
    11041 aagaagaaat ggaacgtgca gctgagattg ctcaagcttc agaattcatt tctcgaaaac
    11101 cagatggatt taatgaacat ttatcagaag gtggttccaa tttttcaggt ggacaaaaac
    11161 aacgattggc aatagccaga gccatcatac gccgtccgga aatctatatt tttgatgaca
    11221 gcttttctgc attagattat caaacggatg cgaagttacg tgctcgtctg aaaaaagaaa
    11281 cgacagaatc tactgtattg atcgtagcac aacgtgtggg aacgatcatg catgcagata
    11341 agatcatcgt attaaatgaa ggagaagtcg tcggtatggg gacgcatcgg gagttgttgg
    11401 aaaaatgccc gatttattat gatattgccg cttctcaatt atcaaaggag gaattagcat
    11461 gaaaaaaacc ttttcttcct taaaacgttt agctcgatat atcagaccgt atcgtgagac
    11521 ttttctttta gtgatcctat ttacagttct gacagtggct ttcaacgtgg ctttgccata
    11581 tgttacagga cttcctacta cagaaatcag ccgtaatatt gccaatggcg aatcaatcaa
    11641 tttttcttat atattcaaat gtctcatttg gatcacagtt gttggaatag ggtattgtat
    11701 ttcgcaatta ctttctggtg ttttgatgac aaatgtcgtt caaagtgcca tgcatgattt
    11761 gagaagagac atagaagaaa aaatcaatcg tcttccggtt tcatatttcg ataaaaacca
    11821 gcaaggaaat attttgtctc gtgtgacgaa tgatgtagat gcagtcagcg gtgctttaca
    11881 acaagctttt attggtatcg taaatgcgat attgggaatt acgttggctg cagcaatgat
    11941 gttttatatc cagccgatga tggcgctgat ctctatgatc atgattccaa catcgatctg
    12001 gatctcaaaa cgagtgatca atgcttcgca gaaatatttt caaagcatgc agaattctct
    12061 tggtgagcta aacggctacg tccaagaaaa tatgacaggt tttagtgtat tgaaagtata
    12121 tggacgcgaa aaagaaacat tggaaggatt caaaaaagtt aatcacagct tgaaatactt
    12181 tgggtttcgt gcagcattca tttctggctt gatgatgcct ctagttcaat taactgccta
    12241 tgctacttat attggtatgg ctgttctagg aagcttttat gttattactg gggcgatcgt
    12301 tgtcggtcaa ctacaagcat tcatccaata tatttggcaa atcagtcagc caatggggaa
    12361 cgtgacacaa ttgtcttcag ttttgcaaag tgcatcagcg gcaacgaaac gtgtattcga
    12421 gattttagat gagccagaag aaaaagaaaa caaagtagat gcttcactgc cagaaacgat
    12481 tttaggagat gtgacattcg atcatgtcga ttttgcatat gatccgaaga aaccattaat
    12541 ccaagatttg aattttgaag tgaaagctgg tcaaaccgta gctgtggtcg gtccgaccgg
    12601 tgccggcaag acgacattga tcaatctatt gatgcgtttt tatgacgtag acaaaggagc
    12661 aataaggatt gatggaatcg acaccaaatc aatgagtcgc agtgatgtgc gttcgctatt
    12721 tggtatggtc ttgcaagatg catggcttta tcaaggaacg attgcagata atatccgctt
    12781 cggaaagctt gatgcgacag attatgaagt agttgatgct gcgaaaacag caaacgtaga
    12841 tcattttatc cgaacgatgc cagatggata tgatatggcc atcaattctg agggagacaa
    12901 tgtgtcttta ggacaaaaac agttgctgac gattgcccga gcagtggtct ccgatcctaa
    12961 aatcttgatt ttagatgaag caacaagttc agtagacacc cgtttagaag cgttgattca
    13021 aaaagcaatg gatcgcgtga tggagggacg gacaagtttc gtcatcgctc accgtttatc
    13081 gacaatccgt gatgcagatc tgattctagt gatggatcaa ggtcagatca ttgaaaaagg
    13141 aacgcatgag agtttattgg cacaaggtgg attttatgaa aaactgtata acagccaatt
    13201 tgctgaggaa gcagaataag tcatataaga ataaaacagt ttaaaactta ttttttgttc
    13261 tctattcttg ataatggtaa agtaaagtta tcaagaaaag tgaggacacg gagatggaac
    13321 agtttttaaa tgaaaaaatt actgaagcca aaagagaagt tgatcgttta acgcacgtct
    13381 gtcagacgaa gccttttgat tcagctgatt tttttgaaaa tgagtttcaa atcgaacgct
    13441 tggcagcaca aatagaagtg tacgaattaa ttttaaaaaa agtaatagaa gaaaatatag
    13501 atgtacaata aaaacaggta aaacgtcagt taccagatac tgtaaataga catctggcct
    13561 gagcaggttt tacctgtttt ttttcttatg aaataaatat atgctatgat acctttattc
    13621 aataggatta gtttctacaa aaaatgaaag gagaaaaata aatatggaac ggtttatagc
    13681 atcaaatgcc acagtgattg gtgatgtgac attgtcagaa gatgtgacga tctggtatca
    13741 agctgtttta cgcggggaca gtaattggat aaaaatagga caaagaacga atatccaaga
    13801 tggtacaatc atccatgtcg atcatgatgc acctgtagat attgcggaga atgtgacggt
    13861 aggacatcag tgcatgctac atggctgtac aatcgaaaaa ggggcgctga tcgggatggg
    13921 gaccacgata ttgaatcatg cggttattgg tgagaatagt ttgattggtg ctggatcatt
    13981 agtaacagaa ggaaaagtta ttcctcctaa tgtcttggct tttggtcgcc cagctaaagt
    14041 gatccgtcct ttgacaaaag aagaaataca aaaaaacaaa gaaaacatcc agcattatgt
    14101 cgagataggt cggatgcatg cgcaaggaaa attcaaggag caaagatagg cagtagcatc
    14161 cataaaataa aaaaggatgg tcagtttgag ttgaccatcc ttttttattt ggtttagata
    14221 agagagctta aaaaagcttc ctttgaaaca gattaattta atgaagaact gttttccatg
    14281 acttcatcga tcaatccata tgcttttgct tgttcagctg tcatatagtt gtcacgatct
    14341 gtatcttttt caatcacttc aagcggttgt ccagtgcgtt cagctaaaat tttgtttaag
    14401 cgttcgcgtg tttgcaaaat atggcgtgcc gcaatttcga tttcagttgc ttgcccttga
    14461 gcgccgccaa gaggttgatg gatcatgatc tcagcatttg gtaaagcaaa tcgtttacct
    14521 tttgtaccag ctgtcagcaa gaagcttccc attgaagcag ccatacccat aacaattgtt
    14581 tggacatctg ctttgacaaa gttcattgtg tcgtaaatag ccatacctgc tgtaactgaa
    14641 ccaccaggag aattgatata aagatagata tctttttcag aatcttgagc gtctaagaaa
    14701 agcaattggg cgatgatcga attagcaaga tcatctgtta cttggccgct taacatgatg
    14761 atacggtctt tcaataaacg tgagtagata tcgtaagctc tttcacctcg tgaagattgt
    14821 tcaatgactg taggaattaa attcataata gagttcctcc ttgatttcgt ttagaaatag
    14881 tgtaccataa tttagtttct aatacctaaa atgtagtata catttttggt caaaaaaggt
    14941 caaataaaaa acttaggcaa attagtcgca taaacccgct ttatggtatg ataagcagag
    15001 aaacaaaaca aggaggaaca acagattgat agttggtatt actggcctta tcggagtgct
    15061 gacaggtgct gtagttgtct atatcggcat gatactgcaa atgcgtcact tggaaaataa
    15121 agaacagcgt agacgtgaat tggaattgaa agtcaaagaa atcgagacgc tgaatcaatt
    15181 aaataaaaag atcaacgaga tcttgcaaaa acggacaacg ctgatggaag aatatgtcag
    15241 ctttgatgca tttgatgatt gttatatcac gattgacgat tttgcgtatc tccaatcatt
    15301 ttcagcgcaa aatagttttt atctgccaaa ttatatttta gacgagctat ttaagaatat
    15361 ctcccatcga aaagtgattt tatctccaga tgaaacaatg aagattggtg gatatgctta
    15421 taaaggcgga cgcgtgattt tagagaattt tacggatagt ctgactgaaa tgatcaatga
    15481 aaaaaaagct cagttgaaga ctatgacgcg ccagccgatt ggtttcttct cgaaagatga
    15541 tgtttgaagc tgatattttg aagcattgcc gttgcacatg agagcgtggt aatgctttct
    15601 ttttttctta atgatacaat agaactgaac ggagtgaaga aagatgttac aaatctatgc
    15661 ccatcgcgga agcagcggca cccacccaga aaatacgctt cctgcatttg cagaagctgt
    15721 acgagtaggt gcagatggta tcgaactaga tgtccatcta agcaaagatg gttatttgat
    15781 cgttatgcac gacgaagaag tggaccggac gactaacggt aaagggctga ttcgagaaaa
    15841 aacattggaa gaactcaaaa aattgaatgc agggggcaaa ttcagcaaag aatttccagc
    15901 agctaaagtt cctgaattga gagaagtgtt aggactgctc ctccaaaaaa attaccgagg
    15961 gatgctaatg attgaaatta aaactgatca gtatgaatat cccggtatcg aagaaaaatt
    16021 agccgaaaca ttaaaaagta gagattggcc ttttcaacat gcatattgca gctttcattt
    16081 ggattctttg gaaaaaatcg cattactgga accggagata cagctggact tattgatgag
    16141 tacttctgat aaaaagatcc aactagcagc agaaacccct tatatagaag gagtacatcc
    16201 aaaaaatact tggttgttcg aacatccagt ccaggcagct gattttccaa aagcaattcg
    16261 tgcatggacg attaacgatg aagaagaaat ggccaaatgt caagaactca aaatagatgg
    16321 aataataact gattttccgg agaaagctct tcggtggaaa caatcaacga aaagtaggta
    16381 aaatatggaa aaagtattag tgatcgtcgg tccgacagca gtcggtaaaa cagcgctaag
    16441 tgtggaactc gcacaactat ttggaggaga gatcatcagt ggtgattcat tgcaaatcta
    16501 taaaaatctg gatatcggta cagcaaaaat caaagaagaa gagatgaata atgttccgca
    16561 tcatttgatt gatgtgatcg aacctactca acgttactca gccgctgatt ttcagcattc
    16621 aggtcgacaa ttgattaccg agattgcgaa ccgagggcac ctacctatca tagctggtgg
    16681 tacgggtctc tatatccaat cccttctata tgattatcag ttaggagcga cagaagaagc
    16741 gaatcctgag attagaaaaa agtatgaaac gttagctgaa acaattggtt cccaagcatt
    16801 atggaatcag ttgaaagaaa aagacccggc agctgctgag aaaatccact ggaacaatca
    16861 acgcaaagtg ataagagctc tggaagtttt agagacgaca ggacatagta tcacggcacc
    16921 taaagaagag ccaaagcaat tgtatgatta cctcatgatt ggattagata cgaagcgtag
    16981 tattctttat gaacgaatca atcaaagagt cgatttgatg ctagcagaag gtttagtagc
    17041 agaagcgcgt aaggtgtatg agcttggaga agtccaggcc agtcaaggta ttggatataa
    17101 agaatttttc ccttattttg aagggaaaga aacattggaa gaagcaacag aacaagtcaa
    17161 actacattcg agaagatacg cgaaacgtca gttgacgtgg tttcgtaacc gtttggatgc
    17221 acagtggttt gatcttataa gtgagccaaa tcaaaaaagt cagattgagc aaacaatcaa
    17281 aaaatggctg gaggcggaat aaaatggaag aaaaagtgat catcgtaggg gtagaaaccg
    17341 aagcaaacca gcgttacttt tcagagtcaa tggaagagct agttcagctg acgaataccg
    17401 catcaggaga agtcgtattc acaattacac aaaagagacc acaagtgaat cggcaaacga
    17461 ttatcggtaa agggaaatta caagaattag ttcaacaagc ggatgcgcat gaagcggatt
    17521 tgatcatctt taatcatgag ctaactccga gacagagcca actgattacc gatgctgttg
    17581 gtcttccggt aattgatcgc gtgcagctta tcttagatat ttttgcgatg cgtgctcgtt
    17641 cgaaagaagg gaagctgcag gttgaacttg ctcagttaga atacctgctg cctcgtttag
    17701 tagggcaagg gaaaacatta tctcgtcttg gcggtgggat cggaacaaga gggccaggtg
    17761 aaacaaagtt agaaacagac cgccgacata tacggaataa aatttttgct gttaagaaag
    17821 aattgaaaga agtagaagct catcgagagc gcaatcgcca aaaaagaaaa aatagcgaga
    17881 tttttcagat tggacttatt ggctacacga atgccgggaa gtcaactatc ttgaatctat
    17941 tgacacaagc ggatacttat tcgaaagatc aattgtttgc aacgttagat ccattgacta
    18001 agagatggcg ttttgctgaa ggatttgaga ttgctgtaac ggataccgtc gggttcatcc
    18061 aagacctgcc tacgcaactg atcgatgctt tccattctac attagaagaa agtcaaaata
    18121 tggatctttt gctacatgta gtcgatgcta gttctccaga tcgtatttta caggagcaga
    18181 ctgtcttaaa attgatggat gaactgaata tgaaagaaat gccgatactg actgtttaca
    18241 ataaggcaga ccagatcgac ccagcgatgt tcacgcctac gctattccca aatgtattga
    18301 tttctactca gacgaaagaa ggtaagatgg cgttgatcga agcaatcaaa cgtcagctga
    18361 tggaactaat gaccccttat acactttctg tgccaagcaa cgaaggacag aaattaagcg
    18421 agttgagaag acaaacaatg gttttggacg aagcgtacat ggaagaaaca aacgaatatg
    18481 aagttaaagg gtttgcaaaa accaattcaa aatggttaag aaaaaaccaa taaaatttt
//
LOCUS       DAXLPI010000050        18198 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXLPI010000050 DAXLPI010000000
VERSION     DAXLPI010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.050"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(45..755)
                     /locus_tag="ACF3T5_001498"
     CDS             complement(45..755)
                     /locus_tag="ACF3T5_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFD3632110.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(773..1269)
                     /locus_tag="ACF3T5_001499"
                     /pseudo
     CDS             complement(773..1269)
                     /locus_tag="ACF3T5_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(1306..1758)
                     /locus_tag="ACF3T5_001500"
     CDS             complement(1306..1758)
                     /locus_tag="ACF3T5_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFD3632111.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWSFVKKGNKRIY"
     gene            complement(1893..2498)
                     /locus_tag="ACF3T5_001501"
     CDS             complement(1893..2498)
                     /locus_tag="ACF3T5_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFD3632112.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(2948..3568)
                     /locus_tag="ACF3T5_001502"
     CDS             complement(2948..3568)
                     /locus_tag="ACF3T5_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3632113.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(3570..4439)
                     /locus_tag="ACF3T5_001503"
     CDS             complement(3570..4439)
                     /locus_tag="ACF3T5_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFD3632114.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANKAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(4462..4869)
                     /locus_tag="ACF3T5_001504"
     CDS             complement(4462..4869)
                     /locus_tag="ACF3T5_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFD3632115.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELHENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(4888..6177)
                     /locus_tag="ACF3T5_001505"
     CDS             complement(4888..6177)
                     /locus_tag="ACF3T5_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFD3632116.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINDQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     ILLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(6204..6506)
                     /locus_tag="ACF3T5_001506"
     CDS             complement(6204..6506)
                     /locus_tag="ACF3T5_001506"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3632117.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(6531..8624)
                     /locus_tag="ACF3T5_001507"
     CDS             complement(6531..8624)
                     /locus_tag="ACF3T5_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFD3632118.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(9113..9841)
                     /locus_tag="ACF3T5_001508"
     CDS             complement(9113..9841)
                     /locus_tag="ACF3T5_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFD3632119.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(9986..10699)
                     /locus_tag="ACF3T5_001509"
                     /pseudo
     CDS             complement(9986..10699)
                     /locus_tag="ACF3T5_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
     gene            complement(10700..12607)
                     /locus_tag="ACF3T5_001510"
                     /pseudo
     CDS             complement(10700..12607)
                     /locus_tag="ACF3T5_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
     gene            complement(12721..14053)
                     /locus_tag="ACF3T5_001511"
                     /pseudo
     CDS             complement(12721..14053)
                     /locus_tag="ACF3T5_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(14019..15497)
                     /locus_tag="ACF3T5_001512"
     CDS             complement(14019..15497)
                     /locus_tag="ACF3T5_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD3632120.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(15802..17637)
                     /gene="tdc"
                     /locus_tag="ACF3T5_001513"
     CDS             complement(15802..17637)
                     /gene="tdc"
                     /locus_tag="ACF3T5_001513"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFD3632121.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            17864..>18198
                     /locus_tag="ACF3T5_001514"
     CDS             17864..>18198
                     /locus_tag="ACF3T5_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632122.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRL"
BASE COUNT         5221 a         3880 c         3097 g         6000 t
ORIGIN      
        1 aaaaaaaaac ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct
       61 gattcatctt tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt
      121 tccttcaacg cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca
      181 atgaccagca ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag
      241 ttgagacagc gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc
      301 ggagcatcgg aatctgcacc taaaggcagg aatgattcgt caccttgggc ttttcctaaa
      361 tgcagatttc ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag
      421 gaaatactat tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt
      481 gcacgtttca gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta
      541 aatgcctgac gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa
      601 tttcttgctt cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt
      661 gttacgtggt tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact
      721 tcccagaaat tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata
      781 attgcttgat gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc
      841 tgtatttgat aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa
      901 caccgagcca aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta
      961 aaaatgtacc gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta
     1021 aaaaatgatc agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg
     1081 caggctcttg atcgatactc actaggaaaa aatctgtttc tttatctttt agctcttctg
     1141 taagtttttc tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtaattatc
     1201 ttttccaaat gtatctgtaa atgtttcgat actaatttgt tgtagtaagt ctacatctga
     1261 aggcgtcatt tttcgaattt cgtatttatt cacggcttct tcctttcaat agatcctttt
     1321 attgcctttt ttgacaaaac tccaatcttc gctaacattc ttgcatactt tatttaaaag
     1381 atattccaat tgttttgctt cttcgtcaga aagaccattt aatgcgactg cattggagta
     1441 gttgttttct cgaatgatga aaggatagat ttctgctcct tttttagtcg gatagagttt
     1501 cttgattttt ttattttctt tatcttccaa acgttcgatc atcccattgg attctaattt
     1561 cttgattgct cgggcagtcg ttgtacgatc tactttgatc atttctgcta gtttttcttg
     1621 gatgattcca ggattttcgc aaatacgtac taaataaaga tactgtcctc ttgttaaatc
     1681 gacttctttg aattcgatat tgctaataga gtctagcgct cgggcaatca ttccgatgtc
     1741 tcgaaggatt tcagccattt catcccacct ttattttgtt tgtaagacta gtttacctct
     1801 ttttgttgca aatgcaacta tttttaataa aaaaatcaag ttctttttta ataactagta
     1861 aagtatattc ttttcttctt ttacgaaaaa gctcaatcac aatagatctg gtttttgttt
     1921 tctaaaataa gtaactgttt cttcgttgtt cgcttttctg atagctttgg caactcttct
     1981 atactaaaaa atcccatgtt ggaagtttca ttattctcaa taaacttggc ctcttcaccg
     2041 tggatcgcac aagcaaaaac aagtttataa tactgaaaaa gctgtggaat atctttttgt
     2101 ttattcgtat caaaaactgc tcgcaactga gtagtttcta ctgttaatcc tgtttcttca
     2161 tagacttctt tacgcacatt ttcttcagga gacaatccta tttcagcaaa tccaccagga
     2221 agtgaccatt ctttcgtccg tttatcttcg attagtagga ccttgccatc ttttttgata
     2281 taggcacgaa catctacttt tggtgttgga tgtccttctc caatcgttgt gagcgctttt
     2341 atttcttcga aagtttcttt tcctatttca ctgatcagtt ccaatgaaat cgtccggatc
     2401 tcctcaaaac gctctttatc aaatacatct tttccataat atagaccagc ttgagaaagt
     2461 gcgagtagtc ttttgtattg agccaacttc ttgttcattc tcttcccttc ttttttagca
     2521 aatgtatgac tatgtaatca agtatagaag actggtttaa ttacggcaaa taaatataat
     2581 gaaatctgca ataaataaaa gaatgttaaa aaactcgaga acaaaagaca ttcgacttct
     2641 ctggggctgt gaataaatgg cgttcaaaag cttgcatctg cggtaataag ccccatcaaa
     2701 cgaaaaatat gacgagctat tcttgtctgg ctgttcttat taattgaggc aggacagctt
     2761 tttcacaacc tccccactaa acggtgttct cctaccaact cctcggtcac aagtcacaat
     2821 attcatcaaa catgagaagc tgtttctttg aaattgctcc ttgtgactcg gagctgaacg
     2881 acaggctcac gacctctcgg taaacggtgt tcaaaagcca atttttcgat attaagccat
     2941 ttctttgtta cttattgtca ttgatttctt tttctaattt atccaatagt ggagtgtaat
     3001 ccacttgagt atagtctgtt acatcaaatt catatagtgt tcctaactgg aatttgtcat
     3061 attttcggtc ggcgatgatc tttttgaatg cttctttcgt aatatgattg gtccctttcg
     3121 ttcgatcttc caatacgtca ccatagtgat aatgatccat gatatagctc aaatgacgat
     3181 ctgattcacg atcacgtttg taccgacgtt gccataacac ttcaaagtct gctgttaggc
     3241 gaatcgtgat cacctgatat ccgtatctat ctgctaattc ttggagtcgt gctttttgct
     3301 tatcactaaa aggatactcg gacacaatca ctttctttcc tgcttccata tacaaatcaa
     3361 gtgctttgta atagaaagga taaactttcg tcctttcttg ttccgcccgt tcttccaaat
     3421 tgttaaaccc gatggattct gcatagtatt ccttgatttc atcgagcgtg atggcataca
     3481 tatctggaaa tcgttcccgt agtttattca taagatatgt ttttcctgta ccaggactac
     3541 cagccagaag aatgaaatac tttttcatct catttatttc ctaagttaat tggactttcc
     3601 aagttcataa acagtccaat cttatgcttg acaatttctt ttactgcttt atttgctgga
     3661 atcacattat ggcgaagaga cttgttgatt tctgtttcac taaaggtttc tttagctact
     3721 ttcgtccagt tgactaaaag ctccgttcca acattgaatt tgcggatccc gttattgatc
     3781 aattcaggat aatcttcttc tttcacacct gttcctccat ggataactag ggggacatca
     3841 actactgcat cgatttcttt cagcaaggga atgtttactt ctgtttttga tttgaattgt
     3901 ccatgattcg taccgatagc aatagctaat gcatccacgc ctgtagcttc tacaaatttc
     3961 actgcgtctt ccggtcttgt atataccttg tcattttcag cgacatggat tccttcttct
     4021 gtaccgccga ttgttccaag ttctccttct acggaaacac cgtgcgcatg ggcatattct
     4081 actactgctt ttgtcttcaa gatattttct ttgaaaggta gatgggaccc gtcatacatg
     4141 acagaagtat atccgctatc gattgcttct ttgatatcat caaagtctcg ggcgtggtct
     4201 aagtgtaaga ctgcatcaac catttcttct tctgcaatag ctgtcattac attgactaac
     4261 acatgcatgc cgatatattt agcggtatcg acacttgttt gaataatgat tggcgaaccc
     4321 atttcttttg ctgcgcctag catggcagga agcatttcta ggttatgcgt attaaaagct
     4381 ccaactgtaa aattcaactc ttctgctgtt tttgttactt ctttcaatgt tgtatacatc
     4441 ttttattgcc accttatctt tttatttgtt tcctttgatc gttacttgtt ttgcaatttg
     4501 tcgcatctca tccattttgc ccatgtagta atcgatccct tgatcatcac cagcacgagc
     4561 agcatctgca cgcttctggt tatagaggct catcagtttt ttatacccat ctccgatcgt
     4621 ctgtttattt tccaagctct tttctaaata ttggatggct tgatcgttct catgcaattc
     4681 agaaaattta agtccaactt gttcataaag ttccgctaat ttatgcctat cttgggtagt
     4741 ggaaatctct tgtttcaatt gtgtaatctc tgattgaatc ttttcggtcg cttctggcga
     4801 tagggctttt tcttcttttg ccagaacttt tttcggttct ttttcttttt tcttaaaaaa
     4861 tccaaacatc attttcccta ctttcaatta aaacgctttc aaaactgggc ttaagatcca
     4921 ggcatataag agtgctgcgg ctactgtatc tacatcggtt gctgtagcat tgatgaatcc
     4981 catcgaatta aagatcgtta ctagcaaagc gggcaataat gtaataaaga aaccatgagc
     5041 aataccgcca atgatcgctc ctcggcgtcc gccaactgcg ttgccaaata tccctgctgt
     5101 ccctccggca aagaaattgg ttagcatacc aggtaaaatc atcgctaagc cgaacatggg
     5161 caaagtaaac atggcaataa tcgtaccaat cgttgtcgta atgaaaccta aaatgacggc
     5221 atttgggcta taaggaaaga aaactggaca atctaaggca gggatcgcat caggaactaa
     5281 tttcatagct atccctcgaa atgccggcac gatttcacct aacagtagac gtacaccagc
     5341 tagcaatata tacacaccca ctacgaattg gatggcttgt aaaaaggcaa acatgatata
     5401 gttttgatct cctgataatt cagaagcgaa tggttcgcct gcaaataaaa cagtgatgat
     5461 atatagcggg accatcacga ccatgacaga caaataagta tcttgcagaa attcgaaatg
     5521 tgtcggcaac ttgatatctt cgatcgactt tttattttcg cctttctcac caaaaagctt
     5581 agcaacaccg gcttcaaata agtacccgat cgtacaaaaa tgacctaaag caatatcatt
     5641 tgatccagtg attttacgaa tgatcggttg cgctactgcc ggcatagcaa tggcgaaaca
     5701 agctccgata aaaccaccga ccaagatcaa tacgatccct cgcagaccgg caaaatagcc
     5761 aaagactgtc gtcatcgttg ccatccataa aattgcttga ccagttaaaa aaatgtactt
     5821 ccattttgtg aaacgagcca aaataatatt gaatacaaaa atggctaaga acgtcagtgc
     5881 aatatctcta cctaaaccca aatcattcat tgcttgatca ttgatagatt caatagaagg
     5941 gatgatccct tgcatatgaa atccttcagt aaagattttc ccgaaataag ttaaacttcc
     6001 aacgatgatg ctcgaaccgg cactcaatac ttgaaatcct aggagtgttt ttaacgtgcc
     6061 agagatgact tgtcctgctg atttcttctg cagtagtaga cccaacatag caatcaatgc
     6121 aatggtgatc gatgcctgag tcaaaatatt ttcgatgata aaattgataa cgctcatgtt
     6181 tttaccctct tttctttttg tatttagata actcccttat cttgcagaac tggggtgatt
     6241 ttttcttcaa tttcattttt tgaaacaata tttttcaaat agatcatcgc cgttttagga
     6301 tcaactgcga acttttcgaa ctgcgaacga aaattttctg ctgtgatgat cacatctggt
     6361 ttcatggaaa ctgcggaaga aatatcacaa tggtccagtt tggcttctac tcctaatttg
     6421 ttcaaaacat cctcagcaga catctgtgct gcaaaactac ttcctaaacc tgcgccgcat
     6481 acgaataaaa tgttcaattt gttcatcttt gttctccccc gtatcattta ttatttctca
     6541 actactgaaa aaagcagttg tttgaaatct tcgatatttg tgcattcaat caatcgatcc
     6601 aacttctctt cgttgttgat caattcaatt agttctttca tgatgttcag atgggagtag
     6661 gagtctactg ctgccaaaca aaaaataatc tttactggat ccatttcagg attaccgaaa
     6721 tcgattggtt gctcaatcgt agctacgctg actcctaatt tattgacccc atcttctggt
     6781 cgggcatggg ctagtgccat gtgtttgcca atcacgatat acggtccgta atcattcact
     6841 gcacgaatca tcgcttcgac atagcgctct tcgacaatgt tttctttaac caatggtcta
     6901 gcagctagtt caatcgattc ctcccaagta gtggcttttt ctttgatcat gatattgctg
     6961 tccgtcaaaa tatcttttaa cataggctgg atctcccttt tgttgatttc taaattgttt
     7021 gtttcgaata atttttctaa cttgccatag atgattcgat ctactcggcc accactttct
     7081 ttcatgacat ctattatttg agtgaataac tccgtcgatt catgactgtt aggctctaac
     7141 cgctgtagtt cttcatttct tttcaaaaag tcttctataa ccaagatatt cgtctcagtt
     7201 aaaatcggat cgacaactaa aagcggctta tttcgataat tcaattgaaa ggtgctgaaa
     7261 accagattga catccaattt atctactaat tcgatttctt ttgaacttaa aactgcagta
     7321 atctcgatct gaggaaactt ttctttcaga ttctctgcta acagatttcc agtagccatc
     7381 ccatggttac agacaacgac tgatttataa acataattca agtctcgctt gattacactg
     7441 gcgatcgtcg aaaagtggat gacaagaaaa gctatctcgt cctccagtat cttgccacca
     7501 attacttctt cgatcgtagg aacaaaatga gtgactgcgc ggtagattgg gaaataattt
     7561 gttcggatgt tttctttcaa tggattcatg acttggatat gattcttgat tcttacaatc
     7621 agtgcagcca tgtgcttata gagattttcg aataaggctt cttcctttag atggaaaggt
     7681 acgtgtgtct cgttctctac aaattggatc aattggatcg ttagtaattg agcttgtacc
     7741 cactcgattg aattggagat atctctagca ttgaacgtct caatagtgaa acgaagataa
     7801 tttctttcta cttgcgggac attgtccaat tgaaattcct cgatcatgtg gtcaataaat
     7861 gcagaaaagc tactgtcttt tatttctgta tttttccaat taatggcaga gataaattca
     7921 tgtttcctga ttcgctggat ccagatcaat gtaaaaagca ggatttgttt tttataaata
     7981 tcatcttctt gtttcaacaa cttattggaa tagatctcct gtattttttt gatttcctcc
     8041 aaaggaaaat aatggagaaa gattttttga gccggtgtta attgtgcatc ttgattcata
     8101 aagaagtcgg tcattcccag attctgattg ataaaatcat agatcatcgt acggatagat
     8161 cgttctgaac ctttataaac cagtccttgc tttccataac tgacgatctc tatcccataa
     8221 ctgaccagtg ttgctcgtaa ccgtctcata tcttcatcta atgtactttt agagatttga
     8281 aattcttctt cttttttgtt tagatggatc gggattcggc tgtaagcaac ggctaaaact
     8341 agatcaaaaa tacgttcgcg ccgattcaaa aagccgtctt tcacatcttt tatttcagtt
     8401 tctaatcgag cttgcttttc cttttccagc ttcaacaaaa aacctttgtt gcgaattgta
     8461 cggatctccg gaagcttttt actgctcaac cagatattcg cttcaagaac ttcctgacga
     8521 atcgtccgct gactgacatt gaactcactt gctagatgtt ttgcttttac tggatattgg
     8581 gctgtcatga ttctgtgaag tatttcaatt gttcttgttt tcatagaaat gatctccttc
     8641 tgatttcaag tatatggttc cgcttacata ataaaaagta gatatttctg ccacttgaag
     8701 tggaaagaaa gtaagcaaag caaacaaaaa ataagccata tgctttttca tctcctcaac
     8761 gaaaaagaca tacgcctatt attacaaaaa ttttgcccat ctatacaaaa aagaggttgt
     8821 gaaaaagctg tcctgcatca agtaataaga acaactaaac taaaatagtg cttcctattt
     8881 tttgtttggt tgttcttatt atcgcagatg caagctttta aacaccattt agtggagaag
     8941 ctgtgacaga gatgttcagc cttgagcgat aagaagcgat tttgaaaaat cgcttcccat
     9001 attttacaaa atcgtgcctt attgccgata ggctatctct ggaacaccgt ttattcagaa
     9061 aaaggagctg cgacaaggct tttgtcacaa ctccgttcat aaatatttcc tgtcaatcca
     9121 gcataatcgg ttgaataaaa tcggcttgcc atcttttttc tttctcagaa aaatcaaaat
     9181 accatatctg agaaaaatac tgtcgttgtg tcttacttcc tgagatcaat cgcccatctt
     9241 ttcgatcgac tgaataatca cagcatctga aatcaattcg gatggcaaaa ctattttcgt
     9301 ggatctgtcg ataatttcct acttcctgaa tagttatttt tttagtatga tttcttacac
     9361 cgtctgcttt gtttttttgc agaactttct gatgttcggc aaacagcttg tctgtataac
     9421 agcttctggc tttttcagga gtttcttctt cccaagcgga ttggatcttc atgaatatct
     9481 tttggatttc caaaaactgt gctttctttt ctttagccgt tcctgacatg cgggacaata
     9541 tctccgattt cagccttttc ttttgtcttt tattcttgac gatagaaaaa gcagaaaccc
     9601 ctcctactga caaaacagct aatgcaagaa accccgtttg ctcataacta gaaggacctg
     9661 tacttggacc ataataccca taagaatctc tgccgtaaga acgactgcca aaataactgc
     9721 ccccatatga acgaccgcca aatgaacctc tgcccgaact tcgaccgcta gatgtacctc
     9781 caccatgacc acccgcgaca gcgaatcctt gggtcggcag agcaaacaaa cataggccca
     9841 ctaacaaaat gattgctaaa taattgattt tttcattgtc ttcctcgtta tcttttatta
     9901 caaaaaacta ttttatcata gccttgctaa aaggaagagt caataaccag atgcataaac
     9961 tgttttttta gcaattttgt cttatttagt cattgagtaa catcggacag atgtatgtgt
    10021 cggcagtcca taattcctct atcttcagca tgaacaagct tgcagacaag agatctgcgc
    10081 ttcctccagg agagatttgg ttttcctgac aaaagtgcaa cagtgctgta aatggacgat
    10141 ttggtaaagc actttgacta gcagcatgag caagtttttg catttccact aacgttttct
    10201 gatcactgcg atgcaatacg catgtgtcat ctagcgaact catcaaaact aacaacatat
    10261 gcatttgttt ctgtacatat gaatcattat ctagctcctt agctgcttgg attgcaagcc
    10321 tgatatgcgg ataccctaat atcgcttctt catatgcacc atttacgcca tatttttgct
    10381 tcaccctatg accatgagtg gtttttttat gacgatattt tgtatcagga aaagcagcaa
    10441 gcttgctgac atcatcaagt attttcgata aagaaaaagg ctgttccata ctgatttttt
    10501 gtgcaatgac actgatcaat agccccattg cccaaatcgc tcctttatgg gtattgatgc
    10561 catttgtagc aatcagcatt gccttttccg cttttcgccc aatctcagca attctttctc
    10621 ttaactcctg atcaatagca taatgccatg ctatttgagc catttcttca aaaaaaggag
    10681 ttagcgtctt agcagactta tccaaaatgc gccgaccttc ttttacggct tttgcgatcg
    10741 tatggaaatt atcatctcct aaaaccatat cagctgcctg tttgcttact tcactacctt
    10801 tgattcccat agcgattcct atatcggctc ttttcaaagc aggtgcatca tttacgccgt
    10861 ctccggtcat tccgacgatt tctccatttt tctgcaaagc tttcacgatt cgcagtttgt
    10921 gttctggtgt cgttcgagca aagacgtcga cttttggtac ttgtcgaatc aactcttctt
    10981 cagacataag atccaaatcg atcccttcta aaactttagc agtatgtttt aagccaattt
    11041 gttccgcgat cgcttttgct gtatctttgt gatcccccgt aatcattttc actgagattc
    11101 ctgcttgttg gcattccttt acagctttga ctgcactttc ttttggcgga tcaatcaacc
    11161 cagcaatccc tgcaaaagtt aattgggtta aagactcatg cgtgagttcc tgtttcgaat
    11221 ctactttttt gtaagcaaat cctaaaacac gctgaccttt tttcgcaaac gctgtggctg
    11281 tttgacccca tgcttcttat tctgaagaag aaagagttga tttagccaat aatacttctg
    11341 gcgcaccttt gacgtaaatc acccctcctt tttcctccgc atgcatcgtt gccatatatt
    11401 tgtattcaga gctgaacgga atttttgctt ctattttttt caaaggcaga tcggctgttt
    11461 cggtgtggcg aatcagtgca agttctgtcg gattgccact taaattttct gttttctgtt
    11521 cttctttcgt ctttaattcc tgacaatttt tcataataaa cagtacgcga gtttcttcct
    11581 ttgctgctac atccacaact gtcatttcat tttttgtcaa tgtccctgtc ttatctgaac
    11641 agatcactgt catagaacct aatgtttcta cagaaggcag tcctttgatg atcgcctttt
    11701 cttttgccat ttcatgtact ccgacagata ggatcatggt caacacagct ggcaatcctt
    11761 cgggtaccat tgacaccacc aaagcgatca tagctgaaag aaggatatgc caatccatgc
    11821 catagcggaa tgttgtaaaa acaactaaga acaagatcaa acaaagaatg ccttgaaaaa
    11881 tttgtttgtt caatcggttg atcttcttga tcagaggcgt cgtttgttgt tcgaccgatt
    11941 gcagtgcttg attgatttta ccgatttctg tcgtgtttcc tgtttctaca acgacaccga
    12001 ctgcagaacc agattgtaca agcgttcctg aaaaagccat atttttttgg tcaccgattg
    12061 attcttctgc tgccaaaacg gaatgatttt tttcagatgc ttctgattct cccgtcaaaa
    12121 tcgattcttc tactagtaga tcatgtactt cgatcaaccg aacatctgct ggaacgacgt
    12181 ctccggcacg taagacaatg acatctcctt ttactagttc tttcgcaggg attttttctt
    12241 gcatcccgtt cgatagtact atcgcttctt gtcccatcag ctgcttcaaa ccattcaacg
    12301 attcttctgc ttttctttct tgccaataac taacgagtcc gttgacgatc actacgaata
    12361 aaataatact tccttcgatg tattcgctcg aagcaaattt caaaaaagag gcaatgatca
    12421 acacgatcat cagcagatca gtaaagtgtt ttgctatttt tttccatcgt tttgtctgct
    12481 gtcttgcttc tatctcatta tatccttgat cagtcaggcg tttctgccgt tcttcttgcg
    12541 acaagccttc tgttgatgtt tgatagtttt tcaatacatc ttctaccgtc aatctgtacc
    12601 agttcatcct atcccctcct gtttaattga tactgttatt atacgcaggg aagttttttc
    12661 aggcgtatcg attatttcag gggggtctga aataattcgt tttgcagaat gtatctgggc
    12721 ctaactgata tttctattgc ttattctttt atatacaaaa tttaagaata caaaccagat
    12781 aaccaataaa aaaggaataa agcgtgtcgt gctttcgaat aacaagctga aagcaattga
    12841 agtaaaaaac accaacaaag caagatcttt atatctttgt gtagtgttcc gtttcttttc
    12901 tttaataaat tgaacatgtg cacacaagat cgaagaccac acaaatagaa aacaactcgt
    12961 tgtcatgcct gccaaaagct gaaaaaacaa tcgactatca gctacaaaaa aaatgtagac
    13021 aaagaccgac aaaaaataac atccctgtaa ttaaaatacc gtgtaaaggt acgcctttct
    13081 tagaaagcgc acaaaaccaa tggggcaaac ttttctcttt tgctgtttga tataaaatcc
    13141 tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa atcattagat
    13201 tgacaattgc tgctccgctt tggcaatgga tcacttgcag catagtcact aaaggacttt
    13261 cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc gttcctatat
    13321 agaaaaagag gatacgccaa ggcagacagt tgattgcatt tttcaaattc ttttgtgggt
    13381 cactcgtctc accaactgtt aatccgatca tttcaattcc agcaaaactg aataaaacca
    13441 taggaaatga agcaatcatt ccagcaaagc caaacgggac taattcattg agctgtccaa
    13501 aatttgcttt gattccaata gactgggtat gaaaatctga gacactcaat cctataccca
    13561 ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat tccaattcac
    13621 caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg ctacctccgc
    13681 ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt gctgttaatt
    13741 ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa aatgcccact
    13801 ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta tttgctaaaa
    13861 ataaatcacc catgacccgc atcaaaccat aaatagccaa accgcatatc aaataaacga
    13921 gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat aatcctgttc
    13981 caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc cgtttcattg
    14041 ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac ttcctgttcc
    14101 caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat gacactgatg
    14161 gctgtgactg caattagatt gatccatgtc ccagctttca tcgtcagtcc gacagaaaat
    14221 ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct ctttttctct
    14281 ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa tctcaaaact
    14341 gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt tacgagcatc
    14401 aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat tgagataatg
    14461 acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt ccaaaaattg
    14521 aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc tggtcctgag
    14581 gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa cagattcaca
    14641 ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc gatattattc
    14701 aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa aatattgacg
    14761 aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac tggatttttc
    14821 aaccgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat gatcggcgca
    14881 aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca tgaaaaactg
    14941 cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc taataagagc
    15001 atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc gatttttgcc
    15061 atatcaaatt tcaaattcaa aaaagtgatg atccaataaa cgactaaaat gacaactaat
    15121 gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa caacggagca
    15181 agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag ccatgagaca
    15241 acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc acctttttgg
    15301 ggaaacattc cggcaaattc accagagatc aatgagattg gaaaagcgta caataatact
    15361 gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg aatatttcgt
    15421 acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat ttgctttgat
    15481 gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt ttgttttctt
    15541 ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa aacccagtat
    15601 caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa acgaacgatc
    15661 caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg aaaaacaggg
    15721 ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt ctcttgttat
    15781 tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt ccattgcttt
    15841 ggcgatttct tttgtataaa aattgaaaat tcgatcatca ttcaaataag gagtcatgat
    15901 agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg ttagacccat
    15961 tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg tagtatgaga
    16021 agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat cgaacatttt
    16081 ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga atacccaatt
    16141 gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc ctttaacagt
    16201 aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg aagccccaat
    16261 caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc agactgcggc
    16321 ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat ctggagcctt
    16381 tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca tatttttatg
    16441 tttgattacg attcctccag cagcataagg gacatagccc atcttatgag gatctatcgt
    16501 gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt caatcgttac
    16561 gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat acggcacaaa
    16621 ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg catcaacatg
    16681 taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga ttttatctac
    16741 actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta gaattgggat
    16801 tttttgttca gctaattcac gaattgtttt ttccaaagca ttaatatcca ttcgataatc
    16861 ctcttgaact gggatagcaa ccatctgatc taagcctact ccgcaaatat ctaacgcctt
    16921 catccaagaa tagtgttttg tttgaggcac cagccatttc cctaatcttt ggatattttt
    16981 tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa agctttcggt
    17041 catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca tttttttcac
    17101 ttgttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcagc gggcgtacca
    17161 taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc cgtctgtcat
    17221 attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag ttgccataga
    17281 agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag ctaataaagc
    17341 aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat gccatgggat
    17401 cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat ggtcgcgggt
    17461 ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat ctgaaggaat
    17521 ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca ttttataaac
    17581 tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat ctttcatttt
    17641 tcttccctcc atattttgtt ggtcaatgtc tcgcagatac atgtgtttat ctgtaacacc
    17701 tcttcactat agcgccaaaa atgagaaatt tttttttata acatcaaatc cacgtagatt
    17761 tttacaacgc gaattgtaaa attaagttag aaaaataaaa aggcatttat ggtacactca
    17821 aattgtattt ccgacgaaag aaaacaaagg agtgtaacca taaatgacct acaaacatct
    17881 taccatagac gaactgacaa tgatagaatc atattatctt caacataata agccggttga
    17941 aatcgctaac cgaatgggac gtgctataca aactatttat aatgtagtca ataagttcaa
    18001 gcaaggcaag actgctcttg attattggca ccagtataaa gaaaataaga aaaaatgtgg
    18061 tagaaaagtc attcaattac ctgctcatga agtagattac attaaagaga aagtcactct
    18121 tggttggacg cctgacgtca ttatcgggcg aaaagaaagg cctgtttcat gcggtatgag
    18181 aacactttat cgtttatt
//
LOCUS       DAXLPI010000051        17195 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXLPI010000051 DAXLPI010000000
VERSION     DAXLPI010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.051"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            52..933
                     /locus_tag="ACF3T5_001515"
     CDS             52..933
                     /locus_tag="ACF3T5_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632123.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            936..1706
                     /locus_tag="ACF3T5_001516"
     CDS             936..1706
                     /locus_tag="ACF3T5_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632124.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            1985..2839
                     /gene="aroE"
                     /locus_tag="ACF3T5_001517"
     CDS             1985..2839
                     /gene="aroE"
                     /locus_tag="ACF3T5_001517"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFD3632125.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            3017..4180
                     /locus_tag="ACF3T5_001518"
     CDS             3017..4180
                     /locus_tag="ACF3T5_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFD3632126.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            4297..4707
                     /locus_tag="ACF3T5_001519"
     CDS             4297..4707
                     /locus_tag="ACF3T5_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD3632127.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            4881..7109
                     /gene="topB"
                     /locus_tag="ACF3T5_001520"
     CDS             4881..7109
                     /gene="topB"
                     /locus_tag="ACF3T5_001520"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFD3632128.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            7280..7564
                     /locus_tag="ACF3T5_001521"
     CDS             7280..7564
                     /locus_tag="ACF3T5_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632129.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            7724..7960
                     /locus_tag="ACF3T5_001522"
     CDS             7724..7960
                     /locus_tag="ACF3T5_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFD3632130.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            7957..8356
                     /locus_tag="ACF3T5_001523"
                     /pseudo
     CDS             7957..8356
                     /locus_tag="ACF3T5_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(9023..9967)
                     /locus_tag="ACF3T5_001524"
     CDS             complement(9023..9967)
                     /locus_tag="ACF3T5_001524"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFD3632131.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(10263..11207)
                     /locus_tag="ACF3T5_001525"
     CDS             complement(10263..11207)
                     /locus_tag="ACF3T5_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFD3632132.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            11428..12642
                     /locus_tag="ACF3T5_001526"
     CDS             11428..12642
                     /locus_tag="ACF3T5_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3632133.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            12664..14679
                     /locus_tag="ACF3T5_001527"
     CDS             12664..14679
                     /locus_tag="ACF3T5_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFD3632134.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(14762..16135)
                     /locus_tag="ACF3T5_001528"
     CDS             complement(14762..16135)
                     /locus_tag="ACF3T5_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFD3632135.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(16211..17170)
                     /locus_tag="ACF3T5_001529"
     CDS             complement(16211..17170)
                     /locus_tag="ACF3T5_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3632136.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
BASE COUNT         5633 a         3042 c         3352 g         5168 t
ORIGIN      
        1 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
       61 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
      121 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
      181 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
      241 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
      301 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
      361 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
      421 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
      481 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
      541 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
      601 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
      661 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
      721 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
      781 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
      841 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
      901 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
      961 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     1021 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     1081 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     1141 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     1201 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     1261 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     1321 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     1381 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     1441 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     1501 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     1561 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     1621 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     1681 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     1741 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     1801 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     1861 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     1921 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     1981 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     2041 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     2101 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     2161 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     2221 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     2281 tttgattggc tataatactg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     2341 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     2401 tgttcaagca gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     2461 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     2521 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     2581 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     2641 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     2701 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     2761 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     2821 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     2881 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     2941 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     3001 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     3061 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     3121 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     3181 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     3241 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     3301 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     3361 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     3421 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     3481 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     3541 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     3601 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     3661 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     3721 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     3781 tctggatggt gtagatatta aaaaaggggg ccagaagatg gatggacatg ttcttttgca
     3841 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     3901 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     3961 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     4021 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     4081 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     4141 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     4201 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     4261 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     4321 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     4381 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     4441 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     4501 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     4561 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     4621 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     4681 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     4741 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     4801 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     4861 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     4921 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     4981 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     5041 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     5101 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     5161 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     5221 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     5281 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     5341 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     5401 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     5461 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     5521 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     5581 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     5641 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     5701 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     5761 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     5821 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     5881 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     5941 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     6001 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     6061 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     6121 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     6181 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     6241 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     6301 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     6361 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     6421 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     6481 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     6541 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     6601 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     6661 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     6721 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     6781 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     6841 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     6901 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     6961 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     7021 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     7081 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     7141 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     7201 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     7261 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     7321 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     7381 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     7441 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     7501 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     7561 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     7621 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     7681 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     7741 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     7801 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     7861 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
     7921 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
     7981 attatcaaaa tcaacgcgaa agttatttta ccgtcactcg ccaggcgaaa tgatcggtgt
     8041 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
     8101 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
     8161 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
     8221 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
     8281 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
     8341 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
     8401 aggaatatgc tgattttttt atgtttaatc atacaaaata gttttttcat aaaaatatgg
     8461 aaaatatgga ttaattttga aataattgat ttaaaaggaa tacaaaagag tgaatagtag
     8521 gaaatagtgg tttagaacta gtgggctgat tttttatata ttttagttta cataatatat
     8581 attatacaaa gtagatttaa aaaatgataa acctctctcg aacggttttt tatcaaaaat
     8641 cgtcattttt ccatcaaaat ctgcttatgc aaaattataa aaaaattttg cagtttttct
     8701 tacttttttt gtcgtttaat tgaatgataa aaaccagttt ctttattcag ttaatcaaat
     8761 atgaacgaat tagatatgaa tcattgtatt tgccgttgcg gaaggatttt ttaattaacc
     8821 acagataaac attgataacg attcgcttat tgtaatcaac agattaaatt tcatatgttc
     8881 atatgtacgg atacattttc ggaccgatta attttaacca ttctgtttat ttgcttacct
     8941 attagatatc aataaaaaac tgcttaacag ctatttcagc tgttaagcag ttttaggatt
     9001 tttagttcat attcattgtt tcttataata cagtgttcaa tacttctctt aaagcacttg
     9061 ttgatttgct gaacattgct ttttcttctt cgttgattga taattcaaca acttcacgaa
     9121 caccattttc gtcaacgatt gatggcacac ctgtaaagat atctttttcg tcgtattcac
     9181 cagttaaata agcagataca ggcaatacag cttgttcgtt attcaagata gcttttacga
     9241 tacgtgcagt actcatacca ataccatagt aagttgcttt tttacgatca atgatctcat
     9301 aagcagcatt acggacttta tcagcaatca catctaattc atcttgggca atacgatggt
     9361 ctttttctac aatttcaaac actggtttac caccgacagt tgtatgtgac catgcagcaa
     9421 cttctgaatc accatgttca cctaagatat agccgtggac gctgcgtggg tcaactttta
     9481 atttcaatgc gatttcttta cggaaacgag ttgtatccaa tgttgttcca gtaccgataa
     9541 cacgtgatgt tggcagacca gattcgttcc aagcgatata tgtcaaaata tctactgggt
     9601 ttgaagcaac aacgataata ccgtcgaatc ctgatcccat gatttctttt acgatttggc
     9661 gcatgattga cgcatttgtt ttaactaaat ctaaacgaga ttgaccaggt ttttggttga
     9721 taccagctgt gataacaacg atatcagcat ctttacattc ttcatagcca cctgaccata
     9781 cagccacatt ttcatcgccc caagccatac catctaaaag gtccaatgct tccccttctg
     9841 ctttttcttg attcacatcg atcaatacta attcattaga gattccttgg ttgatcattg
     9901 cataagcgat acttgttcca acaaaacctg taccgacgat tactacttta cgacttgttt
     9961 ttttcatgaa taaacagctc cttttcataa ctatttgtta ctttcatgaa aagtatagtc
    10021 ttgtttattc attttatcaa catgttaatt ataatagttg ttattataat ttttgatgga
    10081 aacgaattcc tgaatacgca taacagataa atgacaaatg tttacaccta tatcccctga
    10141 taatacaacc aaaagtttag aactgaaaat aaatccaaaa aaattcgctg caacgatata
    10201 ataagacagc aatcgcacct aaaacaattt tccttcatat gctttagtga aagattttct
    10261 tcttaatgtt tatttctcca ttcattggga gtacatgttg aatacttctt gaataatttg
    10321 aataaagaag tcgtttgttt atatccgata cgattagcga tttcttccaa cgaaaaactc
    10381 gtttcttgaa tcagttccaa cgcaaagtcg aattttttct ttagaaccag ttcttgaaag
    10441 gaagcatcaa aatacatttt caatttatta ctaagataga ccggattgta gccaaaatga
    10501 gctgctgttt cttttaacgt acaagaagta aaatgcttat cgatataatc taatactcta
    10561 agcatctcat tttctgcgca tccatctgct gatgaaaagc tttgttgttc taataaatga
    10621 gtcaattcga tcagtaatag tgatgtatag agatttaacg aacggatctt atgactgtat
    10681 gtatcaaaat aaatcaacag catattttca atcaaatgcg tcaaacgact tgtttttccg
    10741 ccgcgaaaat gaatgaattg tgtatgttcc cctttttctt ttgatgcatt tatcaaaaaa
    10801 gaagaaagta gattatttgt gcttgccaaa tgattcaata tctctgtaga taaggtttct
    10861 tcttttaata agatgttgat taaaatatcc tcttgccctg tgtattcgat tctttgagta
    10921 gcttctttat caatgatcac cacgtcactt tcttccatct ctatcctttc tccattaata
    10981 atttgtaaac atgtaccgct atacatataa ttgatttcaa caaaatcatg tgtatgctcg
    11041 ggaacttccg catatcgacg gtgtttactg atatgaatat ttccctgttg aaatagatcc
    11101 cctcggtgga tttttggcgg tgtctgaaga tggtaagcta agtttaaatc attgatattc
    11161 attccttgtt cacgctgcag agtttcgatt gattcttcgt tacgcaaaaa aaatgtctaa
    11221 ttcagctagt tccaattgac tccctccttt ttgtaagcgg tttctttcgc ttattagttc
    11281 acgctgtact gctattatat gcaaagctat ttttggtttc ataaagactt tctatggttg
    11341 ggacatgaac ttcctgtgat tccgctattg agagaataac cacttcatac tatatttttg
    11401 ataagcgctt acaaaaatga aaggaacatg cttatgaata aaaagatgat gaaattttct
    11461 ctgctgtcag tctctcttat tttgacttct gctggtgcag tcacgtctgc aattcccagt
    11521 atgtcagcta cttatgccac gcgttcttta tctgctgttg aattattaac gactgctccc
    11581 tcttttatgg tcactatttt tgttttgatt agtagtttta ttgctaggaa aatcggacaa
    11641 aaacaaactg tgatcatcgg attagtgatt gcatcgattt gtggtatcct ccccctcttc
    11701 tcacaaagtt acagtattgt cttagcttca agagctggac taggtattgg ctttggattg
    11761 atcaattctt tggcagtcag catgattgct gccttttatc acggagatga acgagcctct
    11821 atgattggtt ttcaaagtgc atttcaagga tttggtgcag ctttaatgac atttgttgca
    11881 ggacagctag tacagttcgg ttggcaatat acctttttag tctacgccat cagcttacct
    11941 attcttgtct tgtttgtttt gtttgttcct acacctgaaa caactgaact tcaatcgcat
    12001 ggcacagccc caacaagaca agtaatcaaa acgaatccta aaatgattag ttatggtatt
    12061 tttctcttgc tggtcatcgt ggtatataat gctgtatcga ttaaattagc cacagtcctg
    12121 acgacttatt ctctaggaaa cgaaactaat gcagcgaata tcttaacaat catgcaattg
    12181 gcaagtatgg ctgccggagt ttgttttggc tggcttcaga caaaagcaca tcgctatatg
    12241 cttacacttt ctttactact gatgggaatc ggctttgtat tgattgccac tgtgataaac
    12301 ctgtatgtga ttgggtttgg tgcgatccta acaggaattt ctttttcgtt attcattcct
    12361 tacttattca accaagtaag tatccaagcg cccaagtacg cggagagttt caatacttca
    12421 atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc
    12481 tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg
    12541 ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata
    12601 agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt
    12661 aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc
    12721 cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac
    12781 tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa
    12841 ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa
    12901 catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta
    12961 ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt
    13021 ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc
    13081 gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact
    13141 gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta
    13201 gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat
    13261 gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg
    13321 aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt
    13381 caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat
    13441 tcttctttac tgaatcagat ctggtcagta tcagaagaag ccttcaagtt atgtagtgat
    13501 ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa
    13561 tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta
    13621 gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc
    13681 ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc
    13741 atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta
    13801 gggtttattt atgggagagc aaaagattgg atatttattg attggtctga aatggataaa
    13861 gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact
    13921 tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat
    13981 caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt
    14041 ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt
    14101 ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc
    14161 ttattgaatg acactatcac acagattact actccatatt tcaaattcta tgaacaagat
    14221 gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga
    14281 gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga
    14341 aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga
    14401 gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt
    14461 tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta
    14521 cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct
    14581 gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag
    14641 ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca
    14701 tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg
    14761 cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata
    14821 atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc
    14881 ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag
    14941 cgattttaag atctcctaat actggaagga taaataatgg cagccaacta atcaataagc
    15001 tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac
    15061 cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca
    15121 agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta
    15181 atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg
    15241 gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa
    15301 ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga
    15361 ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag
    15421 caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa
    15481 agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact
    15541 ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat
    15601 tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg
    15661 ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta
    15721 ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga
    15781 atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca
    15841 aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa
    15901 ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac
    15961 tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt
    16021 ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa
    16081 cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta tccattgata
    16141 gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact
    16201 ttttcgtttg tcaaattaag ctagacaaat catctttacc tacgtaactc aaaaatactt
    16261 ccaatggtgt gcgatagtgt aatgattttc taggaatatg atttcgtttt gatgccaccg
    16321 attggataaa ggtttcggat accttattaa aatccatagc tttatataag ccatctttac
    16381 gtaataagcc attcgaatgt tcatttagtc ctcgttgaga aggtgttcct ggatcagcga
    16441 aataaatcga aatatcattg gtattactaa tctttttcca attcgagaac tcttttccac
    16501 aatcaaatgt gatggattta aacaagtgtt tcggtacaga ttgaaaccat tgattcagtc
    16561 tattctcaat atctatagct tgcctaccag cgggtttcaa ggtgatgatc actttagata
    16621 atcgctcaac taacgtgatc accgcacttt tatgttttcc accgacaatg gtgtcacctt
    16681 ctaggtgacc aaactcatga ttgaactgta tataatcagt tgttctatcc tgaatcgaac
    16741 ggcgaaaagc ttgttttcct cttttttctt tatgtccgtt tggttttctt ttccctttca
    16801 ttggtaaatc gtgagggtca aaggttttat ctttaaacat ccgatatagt gtacgtaccg
    16861 aacaagcaat aggaaactct gctcgaccaa taaggacatc tggagtccac ccttgagcca
    16921 cctttttttg aatgtattct tgttgcccat ctggtaacac aatcggacgt cttccgcagt
    16981 tacgtttgtt tttcttatat tgttcatagt agcttattgc gcttccacca gatttaagaa
    17041 aacgatagac attataaatg gtttgtcgag aacgttgtac cagatgggct acttttgaaa
    17101 cagagtgtga ttgattgaaa taagcttcta tcattacgag ttcagttggt gtaagatggg
    17161 tataggtcat ttgcactcac tccttataat tcttt
//
LOCUS       DAXLPI010000052        17011 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXLPI010000052 DAXLPI010000000
VERSION     DAXLPI010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.052"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(29..574)
                     /locus_tag="ACF3T5_001530"
     CDS             complement(29..574)
                     /locus_tag="ACF3T5_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFD3632137.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(586..1278)
                     /locus_tag="ACF3T5_001531"
     CDS             complement(586..1278)
                     /locus_tag="ACF3T5_001531"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFD3632138.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACF3T5_001532"
     CDS             complement(1297..1581)
                     /gene="macP"
                     /locus_tag="ACF3T5_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFD3632139.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1594..2157)
                     /locus_tag="ACF3T5_001533"
     CDS             complement(1594..2157)
                     /locus_tag="ACF3T5_001533"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFD3632140.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2385..3047
                     /locus_tag="ACF3T5_001534"
     CDS             2385..3047
                     /locus_tag="ACF3T5_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFD3632141.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3171..4367
                     /locus_tag="ACF3T5_001535"
     CDS             3171..4367
                     /locus_tag="ACF3T5_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFD3632142.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4468..5844)
                     /locus_tag="ACF3T5_001536"
     CDS             complement(4468..5844)
                     /locus_tag="ACF3T5_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD3632143.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5881..6747)
                     /locus_tag="ACF3T5_001537"
     CDS             complement(5881..6747)
                     /locus_tag="ACF3T5_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFD3632144.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6836..7861)
                     /locus_tag="ACF3T5_001538"
     CDS             complement(6836..7861)
                     /locus_tag="ACF3T5_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD3632145.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7879..8553)
                     /locus_tag="ACF3T5_001539"
     CDS             complement(7879..8553)
                     /locus_tag="ACF3T5_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD3632146.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8737..9630)
                     /locus_tag="ACF3T5_001540"
     CDS             complement(8737..9630)
                     /locus_tag="ACF3T5_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFD3632147.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9704..11401)
                     /locus_tag="ACF3T5_001541"
     CDS             complement(9704..11401)
                     /locus_tag="ACF3T5_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFD3632148.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11394..12707)
                     /locus_tag="ACF3T5_001542"
     CDS             complement(11394..12707)
                     /locus_tag="ACF3T5_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFD3632149.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12707..16976)
                     /locus_tag="ACF3T5_001543"
                     /pseudo
     CDS             complement(12707..16976)
                     /locus_tag="ACF3T5_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333417.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
BASE COUNT         4779 a         3831 c         2952 g         5449 t
ORIGIN      
        1 ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt gtggactcaa
       61 aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggca tctttgaaaa
      121 tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca cagatatctg
      181 tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg atgtctagat
      241 ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt tcctcttgct
      301 catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc gttcctatca
      361 cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga tcatgcgcat
      421 caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga gcagtcagct
      481 ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct gcaacaaaat
      541 catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata cgagatgttt
      601 gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa attcgtcaaa
      661 gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca aaaatgggat
      721 attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag ctaatgcttc
      781 agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg tatcacctgt
      841 aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa ccatttcact
      901 gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc ctgggttgta
      961 gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa cgatgtcacc
     1021 tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca tgttcacgcc
     1081 gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta taccggaacg
     1141 tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc ctgcgcgttc
     1201 ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt ccattgcgcc
     1261 gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga ttgctaaaaa
     1321 aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag agttccattt
     1381 ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct ttttgctttc
     1441 tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt ctgcttttcg
     1501 ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt cgcttcgcgt
     1561 aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca atagttccca
     1621 gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca tcgcttgttg
     1681 cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt gtgctaaagg
     1741 attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat ttgcaaaacc
     1801 tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc cagtttcttc
     1861 ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat cgatttttcc
     1921 agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga caagaagcag
     1981 acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata ccagttctct
     2041 cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa taatttttcc
     2101 gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt tcagcatatc
     2161 ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt tttctcaaaa
     2221 actccttatt agtttaccat agataaacct tagattttct ctaaatatag aaaatccttc
     2281 tttagtccga tactctttat agtagtccgt atgctaaaat aagtatgtaa tcgaataacg
     2341 aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata aaacgaaatt
     2401 ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat agtttttctt
     2461 ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc tttggttcgc
     2521 gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt gaaactcact
     2581 atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca atgaatcaaa
     2641 caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa cttaaagcaa
     2701 tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa ttggtgaaag
     2761 aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg aatctagttg
     2821 atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa cagacctatg
     2881 aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc gcacaagatt
     2941 atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct gttgcgaaac
     3001 agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc ttttgttcat
     3061 aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt ttctaaagta
     3121 gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt atggatcata
     3181 aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat ttattgaaca
     3241 acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag attgatcagc
     3301 tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa cgccaagaac
     3361 aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc atcagctacg
     3421 gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat catgtgcaag
     3481 cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt ctccaagaag
     3541 ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc ttcgggaagg
     3601 tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca caaattgaca
     3661 agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat ctgatgttag
     3721 aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat atcgctgctg
     3781 gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg aaaagagcag
     3841 aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa ttcctagatc
     3901 gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc cagcaagcac
     3961 cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc caagcatcta
     4021 tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc ttgcttcgtc
     4081 aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac ttattgaaga
     4141 aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat gaacgtggaa
     4201 tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca atccaagaga
     4261 cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta gagcttggac
     4321 acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc acctttcgct
     4381 tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc gtttttctta
     4441 tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt gtccatagcc
     4501 atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga tccatgtcac
     4561 agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg gcaatagcgg
     4621 aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc tcaacttcag
     4681 taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta gttccgctaa
     4741 tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg cataagtcgc
     4801 ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa gtaagccatc
     4861 cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg ttaccgtcag
     4921 tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc ctacatacct
     4981 cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga ttcctgtcaa
     5041 gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa tgattcctct
     5101 tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg catcaaatcc
     5161 caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac caaaaggcat
     5221 gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa acaacgcgat
     5281 gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct tggcttcaag
     5341 cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa tgtatgtacc
     5401 attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta aacttttcaa
     5461 aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta gtaaaaattc
     5521 cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa catacagata
     5581 ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac ttagtccaga
     5641 caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac cagcattttg
     5701 attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac caattccaag
     5761 catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat tgagattgat
     5821 ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg aaaacagcga
     5881 ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa ggcaaccaac
     5941 aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa tcccacctaa
     6001 agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac caagtgattt
     6061 gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga tcatgttttt
     6121 ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc ctaagatcat
     6181 tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt tgacgatgat
     6241 ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct taccaaaatc
     6301 aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag tcaaatacgc
     6361 accgcttaca agaagagcta aagcaataat accgtataca taatctttcg tttgtgtcca
     6421 ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac cagtagacaa
     6481 tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat tttgtccaac
     6541 actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga ttacaggact
     6601 aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc ctagtgtttg
     6661 ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc atgccaacat
     6721 agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca tgataagaaa
     6781 cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg attttctaac
     6841 gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc ccttttcctt
     6901 cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt gctaaataaa
     6961 gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta aatcccttat
     7021 caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta actgaaaggc
     7081 tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc ggcgtatatt
     7141 tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca tcggtcaaaa
     7201 cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa taatttgcct
     7261 gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg aggtagtccc
     7321 gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca tcgatttttg
     7381 ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg atcgaacgaa
     7441 ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag tcaatataat
     7501 ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt tcacggatca
     7561 gctgattgat atattcttct accagttttt cttcttctgt cctcccacca ctcaaataat
     7621 tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt ctcttgctca
     7681 agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct aaataagcaa
     7741 ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc gtcaatgcaa
     7801 taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta aaaaaattca
     7861 tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg tttcgatata
     7921 tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga ctgtcaatgt
     7981 attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt cccgacttaa
     8041 gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt tgctcaaatc
     8101 aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa tgccattgtg
     8161 gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta atgcattgat
     8221 ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg cccccatgtt
     8281 catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga tcggtacttt
     8341 cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa gattaatatc
     8401 tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat tgaaatcggt
     8461 cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt gtcgaatgat
     8521 ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct ttcttcttgc
     8581 tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa atgtcatttt
     8641 agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc ttccaagcag
     8701 cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt tctttcgttg
     8761 tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca ctagcaatat
     8821 acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt ctatctttga
     8881 agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt gagtgttcac
     8941 ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa ttgctaatgt
     9001 atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga gcatccgacc
     9061 atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg taaagagttt
     9121 tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc ctccagttgg
     9181 aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat tcgtttcatc
     9241 aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca tatgttgttc
     9301 gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag gatctttgac
     9361 ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct tttcatgcac
     9421 tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt gataattctc
     9481 gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat atgtaaaaac
     9541 tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac tgatttgggt
     9601 ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta aaaaaactgc
     9661 agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact tcagacttca
     9721 ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg attagtaaga
     9781 atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata ttcggtactc
     9841 tttctggccc tggattttct actcctacga tctgaatttc ttcattatag cttccataag
     9901 agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct tctggcgcca
     9961 tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag tatccatttc
    10021 catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta tctgctcctg
    10081 attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca gaacggtctt
    10141 cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca tcatattttt
    10201 caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct tcataatcaa
    10261 attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca tccgcttcta
    10321 gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt gcatctgcta
    10381 atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc gtcacgacaa
    10441 acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc gtctcgccgg
    10501 ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta tctgtgattt
    10561 tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa taaggaatag
    10621 gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa gaaatcgttt
    10681 cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc tctacattca
    10741 tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct gtattgtatg
    10801 tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc atcactggca
    10861 tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa gtttctaaga
    10921 aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg gtagagaata
    10981 ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga tattttaaaa
    11041 accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa ttaagaccgt
    11101 cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact tccgttacgt
    11161 caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta gaaaccaatc
    11221 cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat gtaatcgtca
    11281 cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc ccgaaaatcc
    11341 ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt tttttattca
    11401 tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa aagaattcct
    11461 agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca ttttcctgat
    11521 tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc ttttacggtc
    11581 tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt cccggattct
    11641 aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt tgcatccacc
    11701 caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc gtaaccttcc
    11761 acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg tcctgtcgta
    11821 acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa aggatcattg
    11881 tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc taaataatat
    11941 ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg ttttcctttt
    12001 tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag ataaccgaca
    12061 tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg gtgataaatt
    12121 ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa tccaacttct
    12181 ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt tttcttaggt
    12241 gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc agttgttacc
    12301 gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt gtttctgttc
    12361 gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc ttttttttca
    12421 tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg caccgtctgt
    12481 gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc attttcatgg
    12541 tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa cacgaattcc
    12601 acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac tgctgcaagc
    12661 ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac cttgctccct
    12721 cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc atgaagaatg
    12781 cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac ggattttcag
    12841 gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt tcatattcta
    12901 actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa atctcaactt
    12961 ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca ggttcttctc
    13021 gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca ggtgacttca
    13081 cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg gaagaatcgt
    13141 ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta ttttgtttct
    13201 caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt ttcaaaagtt
    13261 ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca tcgatgattt
    13321 catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca atgataaact
    13381 taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac ccatttggtg
    13441 cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg tcgtaattta
    13501 aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc ccaggcaaac
    13561 gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc gggaaggaag
    13621 aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct tcatggtcta
    13681 attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat gctacccata
    13741 aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca cctgtttgct
    13801 tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg tcattttcat
    13861 caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct gcatagttgg
    13921 tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga tgaataactt
    13981 tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac gtgagtctcc
    14041 ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga tatccgattt
    14101 ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt ggcatattac
    14161 tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt cgcatttcca
    14221 gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt acaacagaaa
    14281 gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt ccgccgactc
    14341 tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca tcttcgttgg
    14401 ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa cgtggtggtc
    14461 cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct cctaaaatcg
    14521 gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt tcgattggtt
    14581 cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca gccaacggct
    14641 cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg taacgaatgg
    14701 aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc tccaggttct
    14761 gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc gcatcaagtt
    14821 ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga gaaaatttat
    14881 aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct ggtggggctt
    14941 gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg ttttctcctg
    15001 gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt gagacattaa
    15061 aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta gaagagatat
    15121 cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc ggtgttacgc
    15181 catcttcttc taaatcagca atatttttca atacatttcg tccaatagca tctagtcgtt
    15241 gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt ggcgggttct
    15301 cttgatactt gtttttcagc caatgatgat aagcaactgt gttgatcgca gatgactttt
    15361 cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca acccagtgag
    15421 tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt acttcatctt
    15481 ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca ttagaagtat
    15541 tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca atcttatctt
    15601 ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt gactgaatcg
    15661 gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt tctggatttc
    15721 tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc cctagtgtaa
    15781 gcggagttcc tccgattggt gtacgattta atgctgtatt ctcacttccc gtcaaacgat
    15841 tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta ttgatagctg
    15901 tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg tcagagagca
    15961 gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt tgactattgg
    16021 tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg atatcgattg
    16081 gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa agggttgttg
    16141 catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct acccattttt
    16201 tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca tttccccaaa
    16261 gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat ccattattag
    16321 tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta tcatcgatca
    16381 agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg ctttgtgtcc
    16441 aatttcctaa cacttttgta tagtcattcg ttgtaaactt catctattct ttcatcaacc
    16501 accacttgaa gaggtagtta cccttcgttc acatcgggga tcttttctgt ttcaaaaatc
    16561 aatgtgccgg tttctgcttc ttttgaataa acttttccga cataccattc ttgaccatcg
    16621 atcgtctcga cttttaggtc ttctccatct tcatcatagg aggatagacc tgttccacga
    16681 acgtttttta cggtaccaat cccggcggct gccgcatcga ttgctaaacg tattctacct
    16741 tcattggcac tttccttttt ttcaaaagcc agatgccatt caagggtttc ttctttttca
    16801 atatacttcc cttcaatttt tgcatagtcc tcatctaaga atacctcatc ctcctttgtt
    16861 tctgttgact cagcagctac atccagggtc gtcatatgat agactgactg gaaatatggt
    16921 ccaaatagca aactgacagt tactaactgc aaaagcttca attttaaccc tttcacatcc
    16981 ttatactcct ttcaaaaata tttgtcttct t
//
LOCUS       DAXLPI010000053        16664 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXLPI010000053 DAXLPI010000000
VERSION     DAXLPI010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.053"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(65..265)
                     /locus_tag="ACF3T5_001544"
     CDS             complement(65..265)
                     /locus_tag="ACF3T5_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD3632150.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(717..1037)
                     /locus_tag="ACF3T5_001545"
     CDS             complement(717..1037)
                     /locus_tag="ACF3T5_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632151.1"
                     /translation="MKKRVLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1540..2658)
                     /locus_tag="ACF3T5_001546"
     CDS             complement(1540..2658)
                     /locus_tag="ACF3T5_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632152.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPEIEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2891..3013
                     /locus_tag="ACF3T5_001547"
     CDS             2891..3013
                     /locus_tag="ACF3T5_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632153.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            3129..4436
                     /locus_tag="ACF3T5_001548"
     CDS             3129..4436
                     /locus_tag="ACF3T5_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFD3632154.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGESRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANMFVIGWRRVAEFFGNLF"
     gene            4555..5754
                     /locus_tag="ACF3T5_001549"
     CDS             4555..5754
                     /locus_tag="ACF3T5_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFD3632155.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5781..7049)
                     /locus_tag="ACF3T5_001550"
     CDS             complement(5781..7049)
                     /locus_tag="ACF3T5_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFD3632156.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(7104..7170)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(7212..7859)
                     /locus_tag="ACF3T5_001551"
     CDS             complement(7212..7859)
                     /locus_tag="ACF3T5_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HFD3632157.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVQALAAEKI
                     RDILDHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(8267..8770)
                     /locus_tag="ACF3T5_001552"
     CDS             complement(8267..8770)
                     /locus_tag="ACF3T5_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFD3632158.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8893..9657)
                     /locus_tag="ACF3T5_001553"
     CDS             complement(8893..9657)
                     /locus_tag="ACF3T5_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFD3632159.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMALTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFMVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKENAESLNVGVAAGIVLYHFSL"
     gene            9765..10040
                     /locus_tag="ACF3T5_001554"
     CDS             9765..10040
                     /locus_tag="ACF3T5_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFD3632160.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEEDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10717..11667
                     /gene="yidC"
                     /locus_tag="ACF3T5_001555"
     CDS             10717..11667
                     /gene="yidC"
                     /locus_tag="ACF3T5_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFD3632161.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11846..12820)
                     /gene="lacD"
                     /locus_tag="ACF3T5_001556"
     CDS             complement(11846..12820)
                     /gene="lacD"
                     /locus_tag="ACF3T5_001556"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFD3632162.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDTKIPGR
                     LPDLLNIWSVKRLKEAGADACKFLLYYDVDEPAEINVQKQIVIERIGSECEAEGIPFF
                     LEILSYDATIGDPSSLAYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QETCYSQAEASAYFYEQSKVTEQPFIFLSAGVNATLFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATPVYVDR"
     gene            complement(12817..13275)
                     /locus_tag="ACF3T5_001557"
     CDS             complement(12817..13275)
                     /locus_tag="ACF3T5_001557"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFD3632163.1"
                     /translation="MTIIHEENIFLQQPLDSQEEAFKFLAKQTFCLGIAHDEKDVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSMIIVTLNKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRKAKSKTKIATILTKEIGEDL"
     gene            complement(13272..14738)
                     /locus_tag="ACF3T5_001558"
     CDS             complement(13272..14738)
                     /locus_tag="ACF3T5_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFD3632164.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFAGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAMSGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSFDPDHAQYNATAAMVKAVGDASMGMMVPVLAAYIAEGIAKRPGLIVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLTILPKQLDGLKAIFLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEHSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLAQGN
                     TTFMAGVSAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKMDDSKAANRTVIEKGRAGDLS"
     gene            complement(14720..15670)
                     /gene="pfkB"
                     /locus_tag="ACF3T5_001559"
     CDS             complement(14720..15670)
                     /gene="pfkB"
                     /locus_tag="ACF3T5_001559"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFD3632165.1"
                     /translation="MSIYTCTMNLAIDLFIETEEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAEDYLCVSGSLPRGLSPKVLIEISRICQEKGI
                     HLIIDSSDQEAMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVELGAENV
                     LLSLGEKGAILFTKDRVFRGNSPKGKVVNTACAGDTMLGAFLAGYMNRRPLDETLRKS
                     IAAGSSTAFRKGLTDFLDVEELSKQIKIREERFERWENIN"
     gene            15813..16562
                     /locus_tag="ACF3T5_001560"
     CDS             15813..16562
                     /locus_tag="ACF3T5_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFD3632166.1"
                     /translation="MDHKLSETEQYLWNFIEHHILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINVLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISQKLT
                     KKDSVIFVSLNGETKELVTAAKNCYDAEIGSILVTANHSSTLKDYCEINLVGFKSEGS
                     YFPDYEVRSRLPLQIIARILLDAYVLRMGEN"
BASE COUNT         4999 a         3500 c         2943 g         5222 t
ORIGIN      
        1 agaaaataga caaaaacgcc cagacacgca aatgtctggg cgtttatttt ttagtatagg
       61 ttaattaagc tttagtaacg tttgttgctt gaggtccgcg ttggccttct tcaacatcga
      121 aagttactgc ttggccttct tctaaagttt tgaatccgtc gccttggata gctgagaaat
      181 gaacgaatac atcgtttccg ttttctgcag tgatgaatcc aaatcctttt tctgagttaa
      241 accattttac tgtacctgtt tccataataa aaatcctcct tgtgtatcaa cacatatttt
      301 gcaattactt gaatggtgta tatctggaag atgtttattt gaaacaagct accacagatt
      361 accgaacaaa aactactcct atagtatatc ataccgttta caaaaaagct agagtttttc
      421 gacttttttt attaaaaaaa gttttctcag cgattacata aagtgaaaaa ccgaactttt
      481 tttaaaataa acgaaatatt gttcagttgg tgtttttttg ctctgatgtt cttctttaaa
      541 ctaaaaaaat tattctttct gactacttat cggttctgtt tcaacggtcg aggctgggga
      601 agattcttta gtaggtctga tgttctcatg gtatttctga tagcctatga cagtgaccag
      661 aagaacaagg caaaccaata cgatcaacga gattagtttt tcttttttgc tcatatctat
      721 acctcataaa taacaggatg atgtccatca tatttctgag tatgagtaaa agcaaaagct
      781 gcagctgtag aatgtaagaa atgccagcaa ctttcataat cacaggtcgg acagtaccaa
      841 aagtaaccaa atcttggctg caaagaccgt tcttctgttt caggaatcgc agaatcaaga
      901 tctgttgtct gtacataaac atttttgtac agacgtgtgc ctgtaatagg agaccgctgt
      961 gtttcttcag caaaaactgg ctgacttgtt aaacttaggg atagaaacag caaacaccat
     1021 aacactcttt ttttcataaa gacctcctta tcgataagtt tttcccaatt aaaatcatat
     1081 ctcaagaacg cccgttattc acattttgtt cataaatgat gttttttgta tcataatcgt
     1141 caaaaagagg tcgtgaccac gtcacaacct cttttttaaa tggtgttcca gaagtagctt
     1201 cttcggaaat aagccgaaat ttcacaaaaa tttgagaaat aatttttgtc aatctcttct
     1261 tatttcttga agctgaccac ttctgtcaca acctcttcct taaacggtgt tcaaaagctt
     1321 gcatctgcgg taataagccc accaaacaaa aaatatgaag agctattttt gcttgctgtt
     1381 cttattactt gatgcagaac agctttttca caacctctca tacacggtgt ttaatcagct
     1441 gaccttcggt attagctcaa aaaacggcaa aatatgtgaa actatttttc cgtttgtttc
     1501 tctaatactc aggtcaaaac actgttttca caacctcttt taataacgaa gaatcgctgt
     1561 cagactcttt tcatctggcg catctttttg atccaaaata aacactttcc cgccattaat
     1621 gatcacatca tctgctaatg cattcaacac ttgacgccga tcatattcta tttcaggatc
     1681 ttcaccgaaa ccatcagcta agtttgacgt agcgataaat agatgcgaca cttttccttc
     1741 tttcgcagct ggaacgatat caactaactg atcgatgaat ttcttatcta agagtttatt
     1801 gtattctttt gattcttgag ctgcgagttc acttgacagc ttcttcgccc cttcttcgat
     1861 atctctaaag gatagctgcg caggagaagc agaaatcgca acgtctgcac aataataggg
     1921 atttttagca atttttttga acatcgtctg attttctggt aatgcaaata aataaatcgg
     1981 ccattgttct ggattttcga aagaatcctt caagtacgta tcaacagctt ggtaatagtt
     2041 gacccaatcg atctctactt cttcatcttt tggatttacg ccatggaaag ttactccatc
     2101 tcgggctccg cctcctccat tagaagagaa gttcaaaccg ccacctgtca gttcagatcc
     2161 caatgcagta acgacatcaa ccggtgcccc ttctggtaga gatacttctt ttacttgttt
     2221 gttgctgact tcatacaatt tcatggagtc gcggttcaat cccaacagat aataggaata
     2281 gttgaactgt ccatttttga taatggccag taaataagga agatcactta catagtattg
     2341 atcgtctacc gggatactca aacgatggat ataggtttgg tcagcagtta taatgaccgc
     2401 tacactcgta gttgcactac gccaaaacga aggatctcct aacaaatttt caattttttc
     2461 ttggaacggg gaccagtcat gttcggaata ttttttctca aaacgtttct ttgcttcttt
     2521 cgcaaaattt ttgaatttga tttgatcttt ttccacatct tgatgggcga catgcgtatt
     2581 cagcatgatc gtgacgaatg gtcctttagc gccttctgaa gtcaattgtg acaataattc
     2641 tttatccttt tttgccataa acgtacccct cctaaaaaaa tcattccttt gtttctatct
     2701 aaagtctacc acaacactat ttttccatac aataaaacgt atctaaaaaa ataaaacggt
     2761 ttctacggtt ttaaatttag ctattttttt aagttatctt cataatttcc tcaattattt
     2821 agccccagtc ttcaactatt cttcatgaaa tgctgataaa ctaaaaaaaa gaataaaaga
     2881 ggtggatctt atgcatttga ttatgatttt cagtttatta agtattatca gtttatttgc
     2941 tttcagcgct tcccgcttat tagatgcgga aacacagcct attcctgtaa aagtacgtaa
     3001 aaacgaccgc taaccttcca taacgtgcgt tcttatccta aagttttttc agaaacattg
     3061 aaactctctc ccctctctga taaaatttta atttgtagcg aaacaaatta attacaagga
     3121 gatcggatat gtccataaaa aaaacacgtc tagtcctatt attggctagt ctcattcttt
     3181 gccttgcttc ttttctacca gctgttgctg tgcaagctga agatacgttc aaagtaaatg
     3241 caaaagcagc ttttgctgta gatgcgcaaa gcggtaagat tctttatgat caagatggag
     3301 aaaaaaccat ggggatcgct tcaatcacaa aaatcatcgg tctttacatc gttcttgatc
     3361 aagtaaaaga aggaaaacta tcatgggatg ataaagtttc gatttcagat tatgcagaaa
     3421 atttgagtat caccccagat ttatcgaatg ttcctttgca taaagaaaac acctacactg
     3481 tcaaagaatt attcgactct gcaatcatcc agtcagccaa tgcttcaatg gtggccttgg
     3541 cagaaaaaat ctctggcagc gaagctaaat tcactgaacg aatgaaagaa caattgaaag
     3601 attggggaat caaagatgca acgatcgtta atgcatcagg actgaataac tcttatcttg
     3661 gcgaaagccg accagaaggt actggagaaa acgacgaaaa tcaaatgtct gctcaagatg
     3721 tggccatcgt ggcccgtcat ttgatcttgg attttccaga aatactggac gtatctagca
     3781 ctacgacaca aatgtttggc gaaaacacgc aatcaccagt ggaaatggtc aactggaact
     3841 ggatgctccc aggattcgtt aattacaagg aaggcgtgga cggattaaaa actggtacga
     3901 ctgagcttgc aggtgcttgt ttcgttggta cgatcactaa aaacgatcaa cggatcatca
     3961 ctgtcgtatt gaatgcggac ggccatgcgg aaaacccgag tgcccgcttc aatgaaacag
     4021 accgtttgat ggactactgc tatgacaatt ggtcagaaaa agagctcgga aaagctaacg
     4081 caagtattcc tagcctaaaa accattgatg taaaagacgg aaaagaatca acagttcctg
     4141 ttgtgcttaa aagccctgta aaagtatggg tacgaaacga tatggatact ggcaaattaa
     4201 cgatcacgcc aacacttgac aagaagcaag tcactgacaa cgagatccaa gcacctatgg
     4261 ataaaggtac aaaaatcggt accgctacga tcaccttggc agacgataaa ttaggctatt
     4321 tagaagacaa caatgcgcca tcaacagaga tcattacgga taaaacagtg gaaaaagcga
     4381 atatgttcgt gattggctgg cgacgcgtcg cagaattttt cggcaacttg ttttaattga
     4441 acaataagaa aagcatcttt ccatcggaaa gatgtttttt tgctttttct agtttaaata
     4501 acagcttttt tcatctattt tcgttatact gtataagaaa aaggagttga atttatgaat
     4561 ctaaccagag atctgatctt tttagttctc tatcttattg gggtattctc tatcgtttat
     4621 tggcgaaaac agaagaagga tccttccttg ttcttttacg gcagtacgtg gacatttacg
     4681 gtaatcttaa cttattttgt cgtgttggaa attttttatt cgacctttta ttttttgatt
     4741 ccattgttgt tctttagtat ctttgctttt tcttatttca cagaaaaacg aaggctgctg
     4801 aatggtcttt tattcaatgt tttcctcatc agtttcggca tctacttgtt tgtcttgctg
     4861 tatgaaacgc aaaatatttt ccttggcgga ctgatcgcct tgatcacgat tccattgcta
     4921 cttgtccttt tatttggtat ttatggattg attgtttttc ttttttggaa cggggtcaca
     4981 gttttacgta gagaatcgca ttcattagcg aatcttttaa cgttgatact agccattttt
     5041 cttacactgt ttcttgtttt tgactttttc ttgttaaaat atttgccgca atggataaat
     5101 gctttgtttt tctgcgttcc cctgatcttg atctatctat ttatcgtatt ctacaacttt
     5161 ttgactgtct cattcttgta ccagttcaac cggcctcgat acaatcaaga cttcatcgtt
     5221 gtgttaggcg ctgggttgat caatggagaa acagtttctc ctcttctagc caagcgaatc
     5281 aataaggcaa ttgcttttta tcgggctcaa agccgtgcta cattaaatcc gcccatctta
     5341 ttgatgtctg gcgggcaagg agcagacgaa aaagtaccag aggccattgc gatgaaacaa
     5401 tatgccatgg aacaaggaat ccctgaaaga gacattcttg tcgaaaccaa ttcgacaacc
     5461 actttggaga acatgctcta ttcaaaggaa atcatggatc agcaaatgaa agggccatac
     5521 cgcgcgatct ttagttccaa caattaccac atttttagag ccggtcttta tgcgcggcaa
     5581 gccaaattga aagcaaatgg tattggtgca aaaacagctt tctactactt gcctaatgcg
     5641 tttttaagag aatacatcgc catcttgatg atgaacaaaa aaagacatat gatcatttgc
     5701 ggattgcttt tcgcaggctc cgcattctta tcgctgatta ctttactttt ctaaaaaaac
     5761 aaggtgtgac aaaacatatg tcacaccttg tttttttctt tcattctctt aactattggc
     5821 aaaaagagta tccctttttt gaccgctact tgctgagcag aataaatacc gctgttgcct
     5881 gaacacataa agctgatcaa gcagatcata aagaagtaaa cagccgcttg tgaaccgaac
     5941 aattcgatcc ccatcacaaa gcaggcaatc ggtgtatttg tcgcaccaga aaatactccg
     6001 ataaagccca atcctgcgag aaaaggaaca gaaacatgga gcaaaggcgc tagtgctgca
     6061 ccaagcgttg caccaatttc aaacaatggt gtcacttctc ccccttgata tcccgcgcct
     6121 aaagacaaaa ctgtgaagaa taacttccca ataaaatcat agacatgtgc ctgtccatta
     6181 aatgattctt gaagtaacgg aagactcaat ccgagataac gttgtgtttg caaaatcaat
     6241 gctgctgcca caactacagc cccacctatc gtatttcgcc aaacaggatg aggaatccat
     6301 tgtgcatacc gtgcttttaa aaagacgatc gaacggctga atacccagcc aatcaaacca
     6361 aaagcaatcc cagcaacaaa aactttgaag aacagcaaca tactccattc agggatcaca
     6421 cccatttggt aatgtaggtg agaaacacca tagctttccg tcatgaaatt tgcaaacagt
     6481 gcagcaaaga agctagggaa gatcgcttct gttcggactt tcccgattgc caacacttct
     6541 aatccaaata atgttccagc taaaggtgtg ccaaatacag aactaaaacc cgcactgatt
     6601 ccactgataa tcaagatttc ccgttctgct ttatccaatc ggaacaaacg tgctgtatta
     6661 tctgctaacg ctccccccat ttggactgct gttccttcac ggccaacaga tcccccaaat
     6721 aaatgggtag ttatggttcc aaacagtgta agtggaataa gccgcaacgg gattttttct
     6781 tctccaccat ttccttgatc gatcactaag ttgtttccac gactagaaag tccgccgtga
     6841 tacgcataga gataagcaaa aagcgcacca ctaactggta gtagataaag gagccaagga
     6901 tgtgatagtc ggatatctgt cacaactgtc aagctattta agaaaaaagc agacaagctc
     6961 cccatgaata taccgagaat cgctgtgatc accagccact tcagcatgta cactaaaatg
     7021 cgtactggac tcggtaattg tttttccata aaaaattcct cctcaataaa aaaacgccta
     7081 ctaaagaaag gggctatccc cagactttag taggcgtcat tagctatctt tcgttagcat
     7141 ttaaaggcga acaccatcac ctattcactt ccattaaaag atagcatatt tttctgtaaa
     7201 aaacaataaa attatcgttc tttctttaca cgttcagcaa attcatagaa aggagccaat
     7261 ttcgttaact gttgattaca ttggtcacta tcatgacaaa tataattccc ctttttcgta
     7321 tacgtgccat cagaacctgc tttcgttgta gaaagaaaaa gcgaaacatt cgatgttttc
     7381 tgacagattg cacagattcc cttatttatc gttggtgata aagtaccgct aatccctttt
     7441 aatttttcat cttcgtaata gaccatgaat tttttctgcg tacctggatc attccagccg
     7501 atgtacgtat gttcttgcaa gtcgatttca ttccaagctg gttgtttgag ttttttcact
     7561 tttcggaata atttctcgat ttgtggtttg ctaggctgca taaacggctg aacgatttgc
     7621 ttcagctcct ccaatgctct ttctgcttgc gcttttgtca gttttacgtc taaaactgtc
     7681 gttaaaaatg tatcgatttc tggcagttcg tgatctaata tgtctcgaat cttttcagct
     7741 gctagtgcct gaactgtttg caccgttttt ggatcattga cagatgagta cgcatttaat
     7801 aactgttcta cttctctttt tataaaaaaa tattggtatg gttttattgt ttgttccatg
     7861 attttaaagt cctttcttct aaccaaagtg catactttgt agaaaaaagt gatcttctgc
     7921 aaagtatgtg ttacctgaag atcccggact cttttaaata agatacgttt tgcatcttat
     7981 catcaccccc tttaatgctt attcttctat gtacatgatt cattcgataa ataaaagtaa
     8041 accgtatctt gaacagatag tcaactgcaa acagaaaaaa ggactgtgac aaaagtcgtg
     8101 tcaaagtccc ttgtttcgaa tgaatggtgt tcaaaagctt gcatctgcgg taataagccg
     8161 aaacttcaca aaaatttgag aagtaatttt cgtaaattcc ttcttatttc ttgaagctga
     8221 ccacttctgt cacaacctca atatggtcta ttattggttt tacctgttaa atcgaggatt
     8281 ctttcccaaa gtaacgttgc catctcttac gcatacttgc agacatgggc tgtgccgctt
     8341 ctttgactgc gcagtatttg tccacgaatg tcacgatata tccttcttta tacttaggcg
     8401 gtgcgatcgt tgcaccccac atgtgcttca aaatgatatc tcgctcaaga tccgacaaat
     8461 cggtgatttt ttctgcattc tttacagcaa ttcttggatg gatatacgca tgtgtcccct
     8521 cgtcaaattt cgtcgtacgc caatcatagt agaacagatc atgcaaaagt ccagcacgcg
     8581 ctgttgcttt agcattgcca ccccattttt ttgcgagcag ataactatag taagaaacac
     8641 tgatggaatg ctccaaacga gtggaatgaa catgctgtgt gtaattcgct aattttttca
     8701 ctgcttctgt ttctaaaaga tcttgtacat aagacatata ttcttcgtct tcacgccatt
     8761 tttctgtcat aattatcgat ctgccacctt tttgaatttc attttcttct gaaattgatc
     8821 taagtatacc accagtgttc ggattattct agtcgctcta gataaaatag ctaaagctta
     8881 atagttcctt aatcataaag aaaaatgata aagaacgatc cctgctgcga ctccgacatt
     8941 caatgattcc gcattttctt tcattgggat atatacgttc tgatcagtca aagacaagat
     9001 ttcctgactt actccctgac cttcgtttcc catgattata gcgaaatcct ctgttttttc
     9061 tactttgtct aatgcatgtg cttcttcatt taattctgta ccgtacacca taaacccttt
     9121 gcctttcatt acagggatca cttctgccaa atctttttgt aacacaggca aatgataatt
     9181 gcttccctgc atgcttcgca gcacctttgt actataaata tctgccgttc ctttacctaa
     9241 taaaacacca gccataccaa acgcatctgc cgtacggatc atcgttccca catttccagg
     9301 atcctgtaca ttatctagta aaagccagcc tcctgaaaaa tctggcgtat cttcagatgt
     9361 taacgccatc actgctaaga tcccttgcgg tgtaggtaga tctgacatag cggtcatgac
     9421 ctcatctgaa actagaaaag tagaaatctc tttgtttaca gccagccagt ctccccattc
     9481 tttcatacct ctctgtgtca caaaaagttc ttttatttct gcatcagacg acgcagcttc
     9541 ttcgatcaga tggaaccctt ccaaaagata ttctgccttt tcttctctgt atttcttttt
     9601 atgtagtttt tttaattctt tgattcttgg attttttgct gattggatct ctttcacatt
     9661 ccttcacctc acctgattat atcatggaat aggtgcttga gcttgtttca tcctaaaaaa
     9721 atcggtatca ttgatttata aaacaggaaa gaaaggtgac gaacatgcgc aaagttaaaa
     9781 tgaatgtcca aggacgtgtc cagggcgtgg gatttcgcta tatgaccaag atggttgctg
     9841 atcagttagg cgtgaccgga acggtaaaaa acgaagagga cggctcagtc acaatcgaag
     9901 caatcggaaa tgacgacatc atccaaaaat ttatcgaaga agtcaagaag tctccgagcc
     9961 caagtggacg agtgcagtat gtggatgtcc aagaagatcc gatgattgag gaacgaaaga
    10021 aatttgatgt ggtgggataa ggttgtgaaa aggtcgctta gttttgagta ttaaggaaaa
    10081 ttttgtgaaa atgtctttcc acatttttgc ataaatttga cttaataccg agaaactgac
    10141 ttttgaacac cgtcgataag gttgtttttg aagcgctttg cctcgagtat taaggagaaa
    10201 cattttcaaa atagcttctc atattttgaa atgtaatgac ttaataccga gaggcagctg
    10261 ataaaacacc gtcaataagg ttgtgaaaaa gctggtctgc atcaagcatt aaggactggt
    10321 tgtgaaaaat agctctccat atttttcaac aggctggact taatgccgta gatgcaagct
    10381 tttgaacacc atcgataagg ttgtttttga ggcactttgc ctcaagtatt aaagacaggt
    10441 agtaaaaaag ctgatctata acttgcaatg aatttgctta acccatattt tccagacaga
    10501 acaaaaaagt ttttagtttt ttaaataaga aagaaacact tggaatcact cttttctttc
    10561 taagtatttc tttcttgatt ttttgtcttt tttcaaaatt tttcgctacg aaaaagaaag
    10621 aatatttata tgaaatttct atgaagttga ttgaattcca tgcgcttttt tagtatagtt
    10681 aagtttgtgt aaaaattata gaagaggaag tattctatga ataaatggaa aaactggtta
    10741 ttgggatccg gacttgtcag tatcctgctc tttttatctg gctgcgtgcg gatggattca
    10801 aacggaaacc ctgacacttc aggaatcgtt tatcgagtac tcgtccatcc tatgggccaa
    10861 gcgattacat atctagttga gaatttcaat tggtcttacg gctgggcagt tattgcaatg
    10921 actgtgatcg ttcgtatcat catcttgcca ctaggtatca gtcaatcgaa aaaaacaatg
    10981 atccaatctg aaaaaatgca agcgttaaaa ccacaagttg aagcagcaca gcaaaagcta
    11041 aaagctgcaa caacacgtga agaacaaatg gcagcacaag cagaaatgca gcaagtatat
    11101 cgcgaaaacg gattgagtat gaccggcgga atcggctgtc tgcctctatt gatccaaatg
    11161 ccgatcttct ctgccttgta ttttacggct cgctacacag aaggtatccg tgaatcatct
    11221 ttttacggca tcgatctagg ctcaccaagt atggtgcttg ttgccattgc aggggtcgct
    11281 tatctgcttc aaggatatat ttcaacaatc ggtattcctg aagaacagaa aaagacgatg
    11341 cgtacgatgc tgatcgtctc tcctgctatg atcgtcttca tgtcgatcag tgcgccagcc
    11401 ggtgttacac tttactgggt agtcggtggt gtgttcagct gtctgcaaac attcatcaca
    11461 aacgttatga tgaaaccacg tttgaaagca caagtggcag aagagttgaa acaaaacccg
    11521 ccaaaacaag tcgtcactcc aagaaaagac gtgacagaat cagcaaagcc agctaacaac
    11581 aaacaagcaa caaaacaaat caaacaacca aaaaacagta caaacaacaa aggccgcaac
    11641 gcaggaaagc agcagaagcg gaagtagata gtaaataaga gtgtaataaa aattcattaa
    11701 cagttttagc aattaaaaat atcgaccaag atttttgaaa aagaaatctg gtcgatattt
    11761 ttatttaagt gaatgtttaa tttgaatcat cgattgatcg ctatctaatt gcagcagcat
    11821 ctttttatga agaaacaact ttttcttatc tatcaacgta gacaggtgtt gctgtttctt
    11881 ctaatagtgt acttaattcc ttaatatttt gttttccttc ttctaacatc caactactcg
    11941 ctttttcttc gccttcggta acaaaaggcg ccactccctt cgcccatgtt gctcgcccac
    12001 ataagacacc attgaatgtc gaacctgatt gttttgcaaa acgtaatgta tcttgaaata
    12061 gcgtagcatt gactcccgca cttaaaaaaa taaacggttg ttcagtcact ttcgattgtt
    12121 catagaaata cgcggatgcc tctgcttgac tataacaagt ctcttgccca tatccttcca
    12181 caaatctcat attcactggc acttctactt tcaatacatc cacttgataa cgttcttttg
    12241 aaaattctcg catggcttca ataactttat gtggttttac ttttgcatag gcaagagaag
    12301 acggatcgcc aatagttgca tcataagaga ggatctctaa aaagaaaggt atgccttctg
    12361 cttcgcattc tgaaccaatc cgttctataa ctatttgttt ttgcacatta atctctgctg
    12421 gttcatcaac gtcatagtac aataaaaatt tacaagcatc cgcaccagct tcttttaacc
    12481 ttttcaccga ccaaatattc aataaatcag gtaatcttcc cggaattttc gtatcatagc
    12541 cggttttttc ataagctagc agcaagccac attctttctc tcgaagcgct gctgcaggca
    12601 ggccgtattc tggatcaagt aaaatagctg aggcataggg agtcaggtat tgagaaacca
    12661 tctgtttaaa ttggatgatg tcttctgatg tagcttcttt atattggttg atcattttct
    12721 tcaaagcacc acgctggtcg attgctaacg cgctaataat tccagcttca tccgtcaatc
    12781 gcttcaaacg ttcctgcttt tctatactca tcttcttcat agatcttcac ctatttcttt
    12841 agttaatatt gtggctattt ttgtttttga ttttgctttt ctcaactgtt ctgtaacctc
    12901 ttttcgcatc aatgatcgtg caacgagaga caataatttc aaatgagtcg ttcctgcttc
    12961 tgaatctgga ataaataacg caaaaataaa atcaactgac tgtctatcta aactgttcca
    13021 ttcaattcct ttgttcagtg tgacgataat catactaggt tcgttgattg tttgagactt
    13081 cgcatgagga atagcaaaac ctgacattat tcctgtagta ccttcttttt ctctttctag
    13141 aagtttttga tatacatctt tttcgtcatg agcaattcct aagcagaatg tttgctttgc
    13201 taaaaactta aatgcttctt cttgactatc caatggctgt tgtaaaaaaa tgttctcttc
    13261 atgaatgatt gtcatgataa gtctcctgct cttccttttt cgataactgt acgatttgct
    13321 gctttactat catccatctt cttgtcttta tcagttactc ttttcttttg gattaatcct
    13381 agtatcactc caccaacgat cgaaccaata agaatcgctg taacccattg gatttttcct
    13441 gttactacag gtaatactaa aaaaccgcca tgtggtgctg gaacttggac gccaaacaaa
    13501 taagtcaaga cagcagcaat tgatgagcct aacatcatcg tgggtaacac tcggatcggg
    13561 tctttcgcag caaacggaat agctccttcc gtaatatgag ttgatcctaa aataaaatta
    13621 accaaacctg cattacgatc attggtatca aaatatttac caaacaataa agtagcaaaa
    13681 ccagtaatca acggtggcgc aatacaagct gctgacacac cagccataaa agtcgtgttt
    13741 ccttgtgcca aaagagctgt tcctgttaca tatgccgcct tattaaccgg tcctcccata
    13801 tcaaacgcac acatgcagcc aacaatcacg ccaagaacaa gtggattcga atgttcaaat
    13861 ccagctaaaa agctcatcat tccttcattt atagccttca ttggacctgc cagcaaagac
    13921 atcacgatgc caataaatgc tacgccaata actggatata agaagattgc cttcaagcca
    13981 tctagttgtt taggtaaaat tgtcagtagc ttttgcaaaa ataagataaa ataacctgct
    14041 aagaacccag ctaaaatacc tcctaaaaat ccagtaccgc cattcatggc tacgattcca
    14101 ccaacaaatc caacgattaa ccctggacgt ttcgcgatcc cttctgcaat ataagctgct
    14161 aaaactggaa ccatcattcc cattgatgca tcgccaactg cttttaccat tgccgctgta
    14221 gcattgtatt gtgcatgatc tggatcaaaa gaatagattc cccaaacggc aaatgaaata
    14281 gcaatcaata ctccgccagc taccacgaaa ggcaacatat gagagacacc gttcatcaaa
    14341 tttttataga tgctacgacc aaaattagca ccactcatcg cttctggttc taaagtatca
    14401 gcagcttcat tatctgctaa gacagtacct tttcctgcca atgcttcgtc aatcagccgt
    14461 tgtgcatcct tgattcctgt actgacagaa acatcaatga ctcgttttcc cgcaaatctt
    14521 tctgaatgga catctttgtc tgcggcaata atgacagcat ctgcctgttt gatttcttca
    14581 gccgttaatt cattttcgat cccaatttgt ccatgggtct ctactttgat cgtgatgcct
    14641 tttttcctag ctgcttgttc taatgcctct tgcgccatgt aggtatgcgc aattcctgtc
    14701 ggacaaccgg ttgctgcaat tagttgatat tttcccatct ttcgaacctc tcttccctga
    14761 ttttgatttg tttacttaat tcctccacat ctaaaaaatc tgttaatcct ttgcgaaaag
    14821 cggttgagct gcctgctgct atgcttttac gtaacgtttc atcaagcggt cgtcgattca
    14881 tgtatcctgc taaaaaagcg cctaacatgg tatcacctgc acatgctgta ttgacaactt
    14941 ttccttttgg cgagtttcct cgaaacaccc tgtctttcgt aaaaagaatt gctcctttct
    15001 caccgaggga taacaaaaca ttttctgcac ctaactctac tagtcgttgt ccataaacaa
    15061 agtaatcttc atctgtaacc atttttcgtc cgaaccaaga agaaagttct tcctcgttcg
    15121 gtttcagcaa aaatggtcga tagggtaaac aatccatggc ttcttgatca ctactatcaa
    15181 taatcaaatg aatccccttt tcctgacaaa tccgactgat ttctattaaa acctttggag
    15241 acagccctct tggtaaactc ccagaaacac aaagataatc ttctgcttgt aatgctctta
    15301 tttgatttaa aaaattatgg acagctgtct gcggtatttc aggtccttga ttcactaatt
    15361 tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc ataccaggca
    15421 cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata tactttcctg
    15481 tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt aatactagtg
    15541 acacattcac tccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg tttaccataa
    15601 aaggatgcat ctcctctgtt tcaataaata gatcaattgc caaattcatc gtgcatgtgt
    15661 agatagacat aaccaatgac ctccttgact ctattatgaa atacgcgaaa gagaaaatgt
    15721 tttcttaatc gttccaacac aaagaaaacc tttacattta taaactcgtc gtgctatttt
    15781 ttaactaact tttggtgaaa gaaggaaaga atatggatca caaattaagt gaaacagaac
    15841 agtacctctg gaactttatc gaacaccata tcctagagat ccctaactac tccatcgtga
    15901 aactaagtga acaagcaaat gtctcaacag ccaccattgt gcggacaatg aaaaagaaag
    15961 gctatgaagg gtttacttcg tttaagcatc atctaaaaga aggagaaaac aagaacatca
    16021 atttttcttt cttagataaa gtagacaaag gtatccgccg agccattcta aaaaatgaac
    16081 aagaagtggt acgtacgatc aatgtgttgg aaatcggaaa tattgaggat gcgatacaaa
    16141 gaatcaaatt tgctgaccgg gtttttgtct ttgcccgagg attttcagaa atgattggcc
    16201 aagaaatgct ggtaaaactt caattaacgg gaaaaaattg tcaaatgcat actgatccag
    16261 aaattatcaa aagcatcagt cagaaactga ctaagaaaga cagcgttatt tttgtttctt
    16321 tgaacggcga gacaaaagaa ctcgtcaccg ccgcaaagaa ttgctatgat gcagagattg
    16381 gttcgattct agttacagcc aatcattcct ctacactgaa agactattgc gagatcaatc
    16441 tcgtaggttt caaatcagaa ggttcttatt tccctgatta tgaagtacgg tcacgcttgc
    16501 ctctccagat tatcgcgaga attctgcttg acgcctatgt attgcgtatg ggggagaatt
    16561 gagaagcagt ttattatggt tggttacagt ttaaaacatc acatttttta ttctttatat
    16621 tgtatagact cacgtaaact ttttatgtat aaagttattc acaa
//
LOCUS       DAXLPI010000054        16195 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXLPI010000054 DAXLPI010000000
VERSION     DAXLPI010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.054"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..475)
                     /locus_tag="ACF3T5_001561"
     CDS             complement(<1..475)
                     /locus_tag="ACF3T5_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFD3632167.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANLNIN"
     gene            complement(605..1315)
                     /gene="budA"
                     /locus_tag="ACF3T5_001562"
     CDS             complement(605..1315)
                     /gene="budA"
                     /locus_tag="ACF3T5_001562"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFD3632168.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1328..2992)
                     /gene="alsS"
                     /locus_tag="ACF3T5_001563"
     CDS             complement(1328..2992)
                     /gene="alsS"
                     /locus_tag="ACF3T5_001563"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFD3632169.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3197..3859)
                     /locus_tag="ACF3T5_001564"
     CDS             complement(3197..3859)
                     /locus_tag="ACF3T5_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFD3632170.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3869..4684)
                     /locus_tag="ACF3T5_001565"
     CDS             complement(3869..4684)
                     /locus_tag="ACF3T5_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632171.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(4946..5389)
                     /locus_tag="ACF3T5_001566"
     CDS             complement(4946..5389)
                     /locus_tag="ACF3T5_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HFD3632172.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5523..5861)
                     /locus_tag="ACF3T5_001567"
     CDS             complement(5523..5861)
                     /locus_tag="ACF3T5_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD3632173.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5849..6226)
                     /locus_tag="ACF3T5_001568"
     CDS             complement(5849..6226)
                     /locus_tag="ACF3T5_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD3632174.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6450..7643
                     /locus_tag="ACF3T5_001569"
     CDS             6450..7643
                     /locus_tag="ACF3T5_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3632175.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7809..8231)
                     /locus_tag="ACF3T5_001570"
     CDS             complement(7809..8231)
                     /locus_tag="ACF3T5_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632176.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8602..9276
                     /locus_tag="ACF3T5_001571"
     CDS             8602..9276
                     /locus_tag="ACF3T5_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632177.1"
                     /translation="MKKLVALFLVAGSLLSGCGATTSTTQSTSSAQASSSSTTISEST
                     ADSSKEQEQIEKKKEEEKQKAEKAALEKKVTEAESALSQAEANPTKENYATASAAIQA
                     LPENKQELTSRLAAIDSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQ
                     AAAEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPC
                     SKCYGN"
     gene            complement(9438..10610)
                     /locus_tag="ACF3T5_001572"
     CDS             complement(9438..10610)
                     /locus_tag="ACF3T5_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFD3632178.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10816..12114)
                     /locus_tag="ACF3T5_001573"
     CDS             complement(10816..12114)
                     /locus_tag="ACF3T5_001573"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFD3632179.1"
                     /translation="MHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYP
                     SKDTAVPADATPFASVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLS
                     LPFYNPNSSDPDKPLDTIHLYAKNVIKESNIKIQKAGNFSGVDKLAGAQFQIKNSENQ
                     FVTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVETKAPDGYQLTNPQ
                     KETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTITVENVQNYGDFHF
                     IKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWSDEVTGSDWEEVKL
                     ISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDDDPTTSDETTIDNQ
                     PKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(12242..12757)
                     /locus_tag="ACF3T5_001574"
     CDS             complement(12242..12757)
                     /locus_tag="ACF3T5_001574"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HFD3632180.1"
                     /translation="MDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFT
                     SSKDGTFSVHGLSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEP
                     KANPTNNGKTPVQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKK
                     QQKGNNRREIK"
     gene            complement(12830..13744)
                     /locus_tag="ACF3T5_001575"
                     /pseudo
     CDS             complement(12830..13744)
                     /locus_tag="ACF3T5_001575"
                     /inference="COORDINATES: protein motif:HMM:NF027869.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
     gene            complement(13741..16068)
                     /locus_tag="ACF3T5_001576"
     CDS             complement(13741..16068)
                     /locus_tag="ACF3T5_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HFD3632181.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4614 a         3236 c         2866 g         5479 t
ORIGIN      
        1 catttatatt caaatttgct aataaaagat ctacatattt ttctagaaat gcttgatcga
       61 tttcatcata ccctttcgtt actccgcaat cgatatctaa aacaccgatt aaattacctt
      121 cttttaccat tggcactacg atttctgatt gtgcggctga atcgcaggaa atgtaatttg
      181 catgggtttt gacatcatca acgatcaatg tttcttgttt ttcagctgat tctccacaaa
      241 ctccttttcc catcttgata cgtgtgcaag aaactcgccc ttggaacggt cctaaaatca
      301 attcattacc atcgaataaa taatatcctg caaagacagt ctcaggcaaa gtctcattca
      361 acaatgcaga tgaatttgct aaatttgcta accagttatc ttctgcttct aataatcctt
      421 gttgctgtaa taataaaatt tcgtatgcat cttttttctg tttttctgtc cacatgataa
      481 aaaccgctcc tttttgatct atggagaaga ttatagctca gtttttttgt aaacgctagg
      541 tttcctcctc aaaaaaacga gatacgtcag gttaatcacc ttgaacgcat ctcgctgtct
      601 ttttctattc tgcttgttgg atttctgctg ctaaatcagc ataatcaatc gtttcgtgaa
      661 caaaagtcgg atcatcaact ggaaaatgct gtacaaggga cgagatgcgg ctaatctcaa
      721 cttttccttc agccacttca aaatccatta catgtccgcc aaatgtatgt gtatcatcga
      781 taaaatgcaa gtgaaatccc gctgcagcaa ctccttggaa taattcaggc gtaaagaacc
      841 cgactaatgt tccttggaca ttgctttgtt ggaattctgg ttgagactct gaaatttttg
      901 ctagtcttct gtaaggtttt tcttgcttcg gcatgatacg gatgtgcata tttttgaata
      961 atccagttat cttcactgcc aaaaataggt tttccccggc ttctcttact aatatgtctt
     1021 ttttcaattg ttctccatct gcagtttttg agacagaaaa actagtatct ggcgtaaaat
     1081 ctgtaattgc tgcatagggg gtaagttcac ttccatctaa ttgggcaact gagccatctg
     1141 atcttccttg ataagctacg ccatctaaaa agatgacttc accatcaagc ccatgcaatg
     1201 ttcccaatcc cagagttcct ttttctaata tatgagcaat cgtttctgtt ccatccatca
     1261 agccagccat caaagctcct aatgtcccgt gctggtataa gattttttca gtcatgtcga
     1321 cacttcctta atagaattga tctggtaaga ttgtttctcc caatttctca ttatcacggt
     1381 aatcaatcgg aatatcaata attacgggtc cttctgtcgc aaatccttct tctaaagctt
     1441 tttctaattc ctctacactt gtagctcgga ttcctttagc gccaaaagct tctgcatatt
     1501 tcacaaaatc aactggacca aagtctacac ctgacgcccg ctgatatttc attttttctt
     1561 ggaactctac catattgtag tgtccatcat tccaaatcaa atgaacgata ttcaattttt
     1621 tacgtaccgc cgtttctaaa tcctgggcag agaataaaaa tcccccatcg ccggaaactg
     1681 atacgatttg tgtatttgga cgcaccaatg cagcagaaat cgcccatggc aatgcaacac
     1741 caagcgtttg cataccatta ctgaataaca aatgacgagg ttcataactt ctaaagtggc
     1801 gtgccatcca aatataatga ctacctacat ctacggtaac agtcatatca tctgttactt
     1861 ttgactgaag cgtgttaata acttctaaag gatgcaagat acctgcttct ccttttgatt
     1921 gaacaatgtc ccgctccgtt aattctgctt gaagagatgc taaatattct tttgcatctt
     1981 ctgacacttg ttgaggctct aatgaacctg tcaataaatc aagagtggct gagatatcac
     2041 cgatcaattc tctttcaggc tgcataaaag gatcgatttc agctggcgct tcatcgatca
     2101 caatgatccg cgcatctttt tccgcattcc agtttcttgc ttcatattcg attggatcat
     2161 agccaatagc aataactaga tcacttcgtt tcaaaagcat atctcctggt tggtttctaa
     2221 ataaacccac acgaccaaag aaatgcgctt ctaattcacg tgagatcact ccagctgctt
     2281 ggaatgtctc tacaacaggc aatgctgtct ttccaactaa ttgcctgatg gctaacgttt
     2341 ctttttcact tgatcctcgc atacctacta atagtaccgg tagttttgct tctctaatgc
     2401 gttcagctag gtaacggata tcatctgcag aagcagaacc taatttagga tcgctcaatg
     2461 gcttgatgac attcccttgg acaggtgcat ccactacatc ttgcgggata ctgataaagc
     2521 tcgcgccttt ttttgaactc ttagccatac gataagcatt ggcaatcact tctgaaattg
     2581 tttctggatc ttggatttct gcactgtatt ttgtgattgg ctggaacagt gctgcattat
     2641 ccatgctttg atgggtcaac ttcaatagat cgcttcgttt tacttgtccg gcaattgcta
     2701 aaactgggtc accttctgct gtcgctgtta ccaaacctgt agctaagtta ctagcaccag
     2761 gaccacttgt tgcgataact actcctggct ctcctgtaat acgtccgatt gcttgtgcca
     2821 taaatgcagc attttgttcg tgacgagtca cgattaattc aggaccttga tcctctaatt
     2881 cattaaatac accatcgatt tttgcacctg gtataccgaa tacataatca acatgatgat
     2941 taatcaaact ttctactaca gatttcgaac cttgtttcat tttttcgtcc attgaacatc
     3001 gtccttttta tgctttattt ttaattatta gttagaaata atcacaaata attgatacag
     3061 tttctaaacc tgtttcattc aaaagtatag cacgttttat ttcatttgtt tagccatatg
     3121 tttcaaaaaa tgaaaaagaa tgacagtttt tacataaaaa ctgtcattct taattgaaag
     3181 tttcaattta taaagtctac agatccatag ttctcatcaa tttacgattg ttgaagtaaa
     3241 tcaaaccaaa tacgatagaa ggacctagta gaatacacca ttggggcttt acgaaaaaga
     3301 agagaaacaa acttgtcaat agtaaaaaat ctagtaaact cacataccat tttttcaacg
     3361 tatagtagaa cgctatccgt agaacttgat ttggtttcga cgctggattt ctcacctgaa
     3421 aataccagta gttgatgcta aaggaaactg ataaacaagc aagtaaaaca aacaacggaa
     3481 tcaaccattt tccataagaa aaacgtataa aaaagaaaca atcaaaaatc attatcacac
     3541 taactatcca agcaaatagc caataaaaaa atccctttag ccaaaattgg cgaaacgtct
     3601 ttatgaacaa acgtactggt gaaaaacttt tttcttttgc taattcttct gtcataccta
     3661 tcatcacgat gcaacctgga ccaataaaaa gtaaagaaaa aataaaggaa aagacattcc
     3721 ttccatctat cgacaaaagg aacgcagctg ctatatacaa gatattgaca caccaaaaac
     3781 aaaggctact tgccaatgat tgatacaacc aataaaagaa ttccatataa acatttttgt
     3841 caaaacgctg ctctttcata atcacctcct attccttcat tcctgatgaa gcgatccctt
     3901 ctacaaaata tttttgcatc gctaaaaata taaaagcaac aggaagaaca gaaacaactg
     3961 aggcagccat gattaatgcg tactctgcat caaatagccc aataaacatt ttcaacccta
     4021 actggattgt tttattttcc gtagaagaca aataaatgaa tggccccata taatcgttcc
     4081 atgtattaac gaatgtgatg atcgtcaaac tggcaatcgc cggttttgta agaggtaaaa
     4141 tgatcttgcg atagattccc cactctgata agccatcgat ccgcgcactc tcacataaag
     4201 aatctgggat actactgtag tattgcttca acaagaacac accaaaagca ttgaacgctt
     4261 gtaatagcac caacgaccac aacgtatatg ttaaacccac ttttctcatc ataatgaact
     4321 gcgggatcat ataagactgc caagggacag caatcgtgcc gatatatgct aaaaataaaa
     4381 tatcgcgccc tttaaaattt agtttagaaa acccgtaagc agcaaaacta gaagtaaaca
     4441 actgaatcaa cgtgatcaca acgcttaaaa aagctgtatt cttaaaaaat gtcactaaag
     4501 gaatacgaga ccaaataacc agataattct ctaaatgcca agttttaggg atccattgaa
     4561 tcggaatcgt gaacacttca ttatttgctt tgaatgaagc agaaaccatc cataaaaaag
     4621 gaatgatcgt caataaagct aatacaatca gcagaatcat acccaaccat tgaataaagg
     4681 gcatgttttt tgaacgaatt tttgcttttt ttctttcatt tcgaacacct ccaaagttat
     4741 aatcttcatt tctttgtaat gattacaatc cagaacaacg gttattatag ctgattcatt
     4801 tccgttcttc ttatgtttgt aagcgcttat ttaacaagtt atgtaaatct ctttattttt
     4861 tttgatttaa aaatgaaaaa atacaaaagc aagactgtca tacgtctcgc ttttgtattg
     4921 aaatttcttc gattagagat acttattact gttcaaaagc ttctttgaaa cggtgaatca
     4981 attcattgac tacaggtgat aaaatccgat tctttttcca gactagcatg cagttcgttt
     5041 caatcattgg gtcaagcgga acgaatgtca ctcgctttga aagatgactt acagcacctt
     5101 ctatgactaa agcagcacct acctgattct caattagcgc aaatacattg aaaatcaaat
     5161 tgtacgttcc tgtgatcagc aactgctcct tttttatgcc taaccagtca gcaaacattc
     5221 gctgtatttc tggacgcttt gagatcaaaa gaggtacatt cgcaaagtct tcttttgtta
     5281 tcacgtcttt ttttgctaaa acagaatttt ctaaaatgag caatccccat ttttcctttc
     5341 ttggtaacaa aatcgtttcg tatttctctg tatctactgg atctagcaaa atcgctaaat
     5401 ctaacaggct tttttctagt cactccgtga tatcatcact tgttccgctg tggatatgaa
     5461 attgaacgct gggataatca cggatcaact tttctagtaa tagtgccaat gtattcgaat
     5521 tatcagcttc cacacagccg atcgtaaatc ggccgctgaa taattgtttt tttctgttct
     5581 cgaattcttg agtggtttga ttcgttaaat ctaaaatttc ttctgctcgg cttttttaga
     5641 aaataacctg cttctgtcaa gatcattttg cggttttcgc gtacaaacag ttctgttcct
     5701 agttcctctt ctaactcctt cagttgtcgg cttaaggttg gttgtgtaag atgtagaact
     5761 tctgcggctt gagagattgt tccttcttct gcaattgtcc aaaaatattt tagtaagcgt
     5821 aactccatag cgatgctcct tgattttttt acttgataca ttccatttta tttctagata
     5881 acacacgatc atatttcttg atttttcctt gtaaacgttc caaacattct tgcatttcag
     5941 caattttttt cgtcaaaagt tcactttctt caatcaaaag cttacgccgc tcaaatacag
     6001 ttgcttctcc ttcgctagat aggcgaatat attcaatcaa agactcgatc gataatccgg
     6061 catttcgcat acagatcgta aagtcgaccc attttaggtc ttcttctgta taatcacgga
     6121 ttccgccttt tgtccgatta actggtggaa tcaacccgat tcgttcataa tagcgcaatg
     6181 tatcggccga aatgcccttt tcttcactga cttgtttgat attcatctga tcactctttc
     6241 tcttaatttc ttgccacttt acttatgtat tttatttatt ctacaacttt aagtgtactc
     6301 caagtcaata ctcatttcta ttaaaactaa gctggattat ctatatcggt tgtttgtgta
     6361 ttatttcaaa taaaaaagtc ttgttttttt attctttttt ggatatactt ttaacaattt
     6421 agggaaattt taggaaaagg ggtataaaaa tgacaacgta tcagcgaaag ttattattat
     6481 cgacatcggc gggaattgct ttagagaata tggatattat gtttttagct ttttcattat
     6541 cttcaatgat tacttctttt catattagtg gtacgcaagc gggtttcatt tcaacgatca
     6601 ctaatttggg gatgctcatt ggaggtattt tttttggcat catagctgat aaatatggac
     6661 gtgtgaaagt attttctcaa acggtccttc tgttttcgat tgcttcttta ttgatgtatt
     6721 ttgcttcaaa catctacttg gtttatctct ttcgctttat cgcaggtgta ggagcgggag
     6781 gagaatacgg ggcgtgtatg tccttgattt ccgaaagttt ttcaaaaaaa caaattggac
     6841 gagcttcttc ggttgctggt attggtgcgc aggtcggtgc ggctttggct gcgattttag
     6901 ctgctgtgat cattccaacg tttggttgga agatgttgta tgtcgtaggt gtactgccgg
     6961 tgttgatggt cttgttcatt cgtcgcggct tgactgaacc aaaggaattc aaatctgtac
     7021 aatctgccga aaagaaaacc aaacttagca gactgtttga atcaaaacat cttgcttggc
     7081 aaacatgtgg ccttagtatc atggtaactg tccaaattgc cggctatttt gggttaatga
     7141 attggctgcc ttcaattatg caaaatcgat tagggctttc ggtttctggg tcttcattgt
     7201 ggatggtcag tactatttta ggcatgtctt tggggatgat gacttttggc ataatcatgg
     7261 ataaattggg gcctaaacta gccttttctt tgtttttaat ttgttcatcc ttatctgtct
     7321 atctacttgt gttagcccat agccagtgga gtttggtagc agccgctgtg atagtcggct
     7381 actttatcaa tggcatgtat ggtggatatg gcgcagtgat tagtagtttg tatccaacag
     7441 aaatccgcgc gacggcgaat aatttcatta tgaaccttgg acgagctgtt ggtggttttt
     7501 cttctatcgt catcggtttc ttgatggatc attatagttt aactgccgta atcttgtttt
     7561 tgagcggtat ttacctagtc agtctgctgg tcttgacaac tcttactggt gttacagcat
     7621 taagggaaag cttgcagcat tgatagttga gtctgtaaat gacaagaaag aggtgtggca
     7681 aaagcttgca tctgcggtaa taagaccagc caaatcaaaa acatgagaca ctgtttccgt
     7741 ttggctggtc gtattacttg atgcagggca gctttttcaa ctgctaattt ccttctatct
     7801 ggcggctact attctttgac gtccgtcatt tcaaagtact ttactaataa cttagcataa
     7861 cgaaaaactg tggatacact cgtatattga gcgtctgcat aatcttccaa atttttctca
     7921 ggatgttgat acatgaattc aatcaaatga taattatatg attttctgta gaaatgaaga
     7981 taaataatgt agattgggaa gttctcctgc ttttccatag aaaagcgccg attattctca
     8041 ttcttcaaaa tgaccttatc aggatatttt tggaacaatt cttctaaatc tgttagataa
     8101 ttcaagatag acttacttga taccttcaat tcgttttgta gttcttctat tgaacaccaa
     8161 cgttctgttt caagcagtaa atgaatcatt ttaattttct tttgagaaac ttttgttaaa
     8221 aaagtctgca tattttctcc tcctgtatct actttactat actacataca agaaaaaaaa
     8281 cggatacaaa tatcatattg tcacaccgtc aatagtgcat aaacctgtta ttatttctct
     8341 ttttttccta agtattctat tgatatgaat catttaatcg tttattatat aaatgtcaat
     8401 aatctaataa taaaataacg tacatatttt attattgcat acttaataac agaactatag
     8461 caaaaaatcc cgtgtatcaa aacgggatta gattaagttt cctcaatcac tattcataac
     8521 acaattatta taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa acggttgttt
     8581 tttacttaag gaggaaattt agtgaaaaaa ttagttgcat tgtttttggt cgcaggaagt
     8641 ttgttgagtg ggtgtggtgc tactacgtct actactcaaa gcacaagttc agctcaggct
     8701 tccagcagca gtaccacgat ctccgaaagt acggcagatt catcgaaaga acaagaacaa
     8761 attgaaaaga aaaaagaaga agagaaacaa aaagcggaaa aagctgcact agaaaaaaaa
     8821 gtgacagaag cggaatctgc tttgtcacaa gctgaagcaa atcctacaaa agaaaactat
     8881 gcaactgctt cagcagctat ccaagcgcta cctgaaaaca aacaagagtt gacaagccgt
     8941 cttgctgcaa ttgattccac aatcaaagca aaagaagcag aagaagaacg gaaaaagcaa
     9001 gaagaaattg cacgacaaca agcagaacaa gcgcaacaag aagcaatcgc acagcaacaa
     9061 gcagaacagg cgcgacagca agcagcagct gaacaggcct ctcagcaggc acaagccgca
     9121 gctcctgagc aaaataatga acaaactgtc tatgtcacac cgaccggttc aaaataccat
     9181 acacataaat gtggaaatgg tacgtatacc cctgccactt taagcgaagc tcaagctcgt
     9241 ggtttaaccc cttgctccaa atgttatgga aactaattca aaagttatac tataataata
     9301 aaattcttct gaaacataaa aaaatagaag ctggcgttcc agatagtaaa ccaaaaatag
     9361 cagtttacac gtccatttcg tcagcttctc tcttttttca tttagtctgt tccctctgct
     9421 tttttggaca tcttcattca ttctttctca atcaaatagt acacctcttt ttcttttcgc
     9481 agtttatatc ttcctttttt caatttcatt gaaaactgtt tcgaatgttt atgttttaca
     9541 tggattttaa gcaggagctc ctcatgttgc aagcgatagt tcctgccttt taaatcagaa
     9601 aatctcacta gtccgtattc atcagaagct ttcacaaatg gaactttttc tctagtaata
     9661 tagtgtttgc cgttgagcgt ttttacagaa aagaccattc cttcgattgg tcgtccgttt
     9721 gtgttcttta cataaaaaga taaaggatag tttcgcttag cgatggccaa agaatctaaa
     9781 taagcgatga gtagccaaac taagccgatt gatccaagta gtataccgat aaaccaaata
     9841 aaaaatttcc attttttcca ttgatcaatt ccttggatcg ctttttttgc ctcttttgct
     9901 tgataaggaa tacgatgccc cgtcacaagc agtcgatgtg tattgaccat ataaggagta
     9961 caggtaagca aagtcacgag atctctgcct ttttggatac ccaaagaatc tacttcatca
    10021 ggtaagacca cttggatttt ttctacttga tacgccaatg tctttccatt gatctggatg
    10081 aaaaactggt ctcctttttt caacttaggc aaatctgtaa ataattttgc ttctgggagt
    10141 ccacgatgcc cagaaagaac ggcatgggtg tttttcccgc cagtcggata agaactttct
    10201 tccaataatg acgttccttt ttgcaaaaat atttccgttg tctgatcgaa tataggtaag
    10261 ctgacattga tttttgggat ttcgatgact ccaagcatat gttccataaa aaaggcatta
    10321 ctaggtaaat cttttaatgc ttttgcatca actgcttggt tgaaactgcc tatccctggt
    10381 gatacgtttt gctctgccaa ctgttggttt ttctttgtca ttttttcctg ctgttttttt
    10441 atttcctctg aattcttatt cgatgcttgc ttttgataat ggatgatcat ttgctgggca
    10501 agaaaatcat tcagtgaatc ttctacaaaa ggataagaaa agatcccgat cccaacgact
    10561 aaaatgattg ccatgaagat gtccagtatt cttattctgc ttttattcat catgtccacc
    10621 tattttctaa tatgtatatg aagaggttgt gaaatcagcg ccttgacctg agtattcgct
    10681 caacaaacgg caaaatagtt cctcctattt tgccgtttgt tgagcgaata ccaagagacc
    10741 gaccacttgt cacaaccttt ctccccattt gaatcactat cgtttattta tcgtatgatt
    10801 tttgtttcca atagtttaag cttctgtttc ttgatggcgt tttttgaagt agatcactgc
    10861 tcctccaact aaagcagtac ctaccaagat gaatacgatg atccccatac caccagttga
    10921 aggcaagatt cctttaggtt ggttatcgat cgttgtttca tcagaagttg ttggatcatc
    10981 atctactgta aattcagtgt acgcagcttc attcttaggt aatgcgtagc cttctggtgc
    11041 ttttgtttct tgaaggcagt atgccccttg tttcagccct tcgattgaaa atttcccttc
    11101 tgaatcagaa atcaatttca cttcttccca gtcagaacct gtgacttcat ctgaccaagc
    11161 atatgggtat tctcctgagg caagatttgc tatttttttc attaatttaa cagttggatc
    11221 ttttgctctt agaatagaaa attctgcatc ggctaatttt tcttgcgtgt tcacatcttg
    11281 tttgatgaag tggaaatcac cgtagttttg gacattttct acagtaatcg tgttatccgt
    11341 agttgtattt tcccgattga ctacaccgtt tggattccca agattggctt catctgcgcc
    11401 atggatgata ccggttgttt gttgcaattt tccgtttttt actataaaag tagtttcttt
    11461 ttgtggattg gttaattgat aaccatctgg tgcttttgtt tcaactaaag tataattgcc
    11521 gtctattaat ccattgacac tgatggatcc ttcagtatca gttgtgaatg tcaacgctga
    11581 ctcagcagga tcatagagag cgacaccagt gttttcatca aaaccggtca cgaattgatt
    11641 ctctgaattt ttgatctgga attgcgcccc tgctaactta tctacccctg aaaagttccc
    11701 agctttctgg attttgatat tgctttcttt gatgacattt ttagcatata ggtgaatcgt
    11761 gtctaaaggt ttgtcagggt ctgatgaatt cgggttataa aagggtaagc ttaacacgat
    11821 atttggcgac gtttcgacac cgggctttgg tgtttctaca aagacgtata cagcatcttt
    11881 cccatcagat ttacctggaa gagtcaggtt agctaatcct tcaccagaag tcgttacact
    11941 tgcgaagggt gtagcgtctg ctggaactgc tgtgtcttta cttggatagt acgtagcagt
    12001 gatgtcatat acgttgaatt caacgccgtt caacggttcg ctattcggaa atgtcaattc
    12061 cgttccgttg tttatttgat tttctggtgc tgaagtgaat ttcaatttat gcaaattaac
    12121 cgtaacattt tctggtgtag gtgatacggc ctagaaacct ctgcagttcc taggccgaga
    12181 acgaaaggaa gcagcatgat agcagttcct aaaaacgtcc atatttttgt tttgatgttt
    12241 ttcattttat ttccctccta ttgtttcctt tttgttgttt tttataaaac caagctgtcc
    12301 ctgttgaaag gactagcaac aaccctaaaa ttgagaaacc tccaagcatc tcttcgcttg
    12361 tttttggaaa gcttcctcca tttttttcat ttgatggacc atttgtctgc atctgaactg
    12421 gcgtttttcc attattcgtc ggatttgctt ttggttcgtt gatgatttcc aatggtatag
    12481 gatgaagcgg atcataactg cctttttcaa cttcaaagag aatggttgat tgactcaata
    12541 tatatccttt aggcggttta atctccacta gttcgtaact tccaaaagac aatccatgga
    12601 cagaaaatgt tccatcttta ctggaagtaa aaatcgttgc tcttgaatca tctttggcta
    12661 cccaatgatt tatcccgttt tcttgcgcta agtattggtc attttttttg actagaaatt
    12721 gagcatctgc tagtttttgt ttcgtttgcc gatccacttt gacaaattgt ttgcctcctg
    12781 tttggatggc aagatgtgtt tctactgttt gattgtcagt cgtcaaacgt acatcgttat
    12841 caaatgtcgt tttttcactg ttttctttca atgtcatttg gtaacggatc gttaattttt
    12901 tcccagcaaa cgaactaaga tcttttccag aatctgataa gacaaaacca tgttttgttt
    12961 tttgtgtgtg aaatgtatga atcttgtctc caccaatcaa aaaatcgatc gtttctggtt
    13021 ctagccataa ctgaggatca gctgtatctt tgatcgtata ggttcctatc tcgttgatgt
    13081 tctcaggaat tgttacggtg atgagataag gaactttatc cccatctgca aaatctgtct
    13141 gcttattctc tactgttttt gttaatgtga gtgcagcagg tattttttgt tcgctctttg
    13201 gatacaagtg gatggtcgtc aatttatgat tagatgaatc tgtcacagga agcacaacga
    13261 caaatggtgc tgatttttct tttacttaat ctgaagtctt tatttccgca aatcggtaaa
    13321 cagcgtcacg accttgctca tctttgtccg atgcactaaa agaagcaatg ccctcttcct
    13381 cgtctactgt ttttgtcaca ttttctgcca gtattttttc actatcactt ttttgtgcta
    13441 gttttctttg tgcgtcttcc acactacttc cttctgaccg cagcttgtaa aattcatccg
    13501 agacatcata tatagcgaaa gtaacgccgt tcaacccttt tactccttga aagttatccg
    13561 ggatatttat tccgtcgttt tgactgatac ttggcatctt atcgctttcg aacattcgat
    13621 tatgcaaagt aaatgtaata ggtgattctc tagctgacac agtcggagaa cctataaaaa
    13681 agaccagtag gataaaaaag agaacagcaa atatatccac acgtacattt tttgtttttt
    13741 tcatcgctgt tcacctgctt ttttcttacg atggataaaa taaaagaagc cagaaaaaat
    13801 aaagatcagt cctaaaaaga caaagggcag tagacctatc ccacctgtcg aaggaagcaa
    13861 gagagtcgga ttgatcgtca acttagcggt atctgttgcg ataatcgtat aattattgtt
    13921 tacacttcct tcgcctaaac taatcgttac tatttgtgat ccaatatctg tccggctgtt
    13981 aactgttagg gtataatcca atgattcgcc taagacaaca ccggtaacgt ggagatttcc
    14041 aaccgaaatc gagtgacttt gggcatcata attaaaggca gcgtcatcga cagtaatcac
    14101 tgcgttagct ttatagatgg tataagttcc tgatgtgttg gctatatcag taaatgacaa
    14161 cttgggattt gctgctttga ctttgtctat tccgctattc gttagtttaa ttgtatagct
    14221 gccagcatta accatatttt ttgctgattg tgcatctgct gtgtaatcca tactgctaag
    14281 tgccacttgt actggagtaa gcgaactagt gacaccctct gctagcttga catggattga
    14341 cgggacacct gcttttccat catatgtctt actgccactg agctctaccg caccgctgat
    14401 ttgcttaatc gtatagacat cagatgtctg cacgatagta aataaatagt ttgttctggc
    14461 agctagttta gctagtccgc tttttgtcaa ttgccaagac gtataggttc caatttcagt
    14521 aggcgcagtt acttcaactc ccttttcatt catcgctttg aaatcagttg gattccatgt
    14581 gatttgatca atactgagat cattgtttaa aagggatgtt gattctgttt tatcattaaa
    14641 ggcatattta acgatcggta caacagaaag accatcatag tacttactgc catttgcatt
    14701 gattgatact gttgcatttg tcttataata taggatgact tcagaaatct gttttccatt
    14761 tgcgtccttt acgattacat ttccatttgt atctaaacta gctaaaacac tcattgctga
    14821 ttggttcttt gtttctgtcg ctgaaacagc tgtaaaggta tatccgttga tagtaggcgt
    14881 attagtcgtc aggtcttgtg cagcacttcc gtaagtaaga ttcattattt tagaaggagc
    14941 aatcatctgt ccaaaatagt cttgatactt aatagtgatt ggtgtttgtt tgcctgtata
    15001 ggtcaccgta tcgacgttac tttctagggc tgtgaaagaa ccgtttgttt gtgcaaaaga
    15061 atagcccgtt actgctgttg gagtgataac cactttgtca cccacatgga ttccagataa
    15121 tgtagtcccg tacgttccga tttttgctgc tccaccattg actgtataag aaaaagttcc
    15181 tgtccgattc gcattgacct gaaccgttgc tgcttgatag gttggaaaat ttgaaacatt
    15241 tcccttacca tcaacgatct gcccattttt gatcgagtac cccgtgaatg gaatatcatt
    15301 tgctaactgt ataccgattg aaaaagtact catacctcct ccggcaggaa gccctcccca
    15361 tgttgagggt gtaaaagaat aacttttatc cgcattgggt gtaactgaat cgatcgtatt
    15421 tccagcggta gccaccccca tatagatact ttttgggaat gtgatagtag taccatttac
    15481 tgtggagcca ataaacagat tatggatatc tggacggtta tcaataaaag cgattgcttt
    15541 ggttacgcct tgtaacgcag ttttatccgc actcatcgat ccgttaaccg caacaatgac
    15601 tccatctttg gcatatatca ctgtatatcc atctggtatg gtatgtccgt tgaaattcgc
    15661 agtggccgca acagtactaa atgtctggct tctacctgtt tctgaataag ctttctcccc
    15721 tgctgctttt gctgctgtga tttctttttc tgtcggatca atcagttgag tcgaggatgt
    15781 ggttgaggtt gtctcgttgt tgctagactg attggtgctt tctatggact gtatttcaga
    15841 atagggtgga ctatttgtct cctctgtcgt ttggttagca aaatttgacg tttctgtata
    15901 aacgtttaca taaatacttg ataagctccc taagaaagta aaaatcaaga tgatcgaaat
    15961 cataatggga tgtaagtatt ttcttttaga tacagctttg agtctagaaa caaaatgttt
    16021 ttgacatata cgttggattt tattttgagt tttcttaatc attttcatcc ctcctctcaa
    16081 taaaaaatca tcaaataatc ctttacacgt tattctaaat ttaattttta ttattttatt
    16141 gatgtataga aagtttgggg ctttttttct ctttttattc taaggttatt ttagt
//
LOCUS       DAXLPI010000055        16172 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXLPI010000055 DAXLPI010000000
VERSION     DAXLPI010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.055"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(49..882)
                     /locus_tag="ACF3T5_001577"
     CDS             complement(49..882)
                     /locus_tag="ACF3T5_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFD3632182.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLAKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEEGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(1432..3597)
                     /locus_tag="ACF3T5_001578"
     CDS             complement(1432..3597)
                     /locus_tag="ACF3T5_001578"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFD3632183.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNRQADS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLEVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3857..6151
                     /locus_tag="ACF3T5_001579"
     CDS             3857..6151
                     /locus_tag="ACF3T5_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFD3632184.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFAGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEATITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            6144..6809
                     /gene="pgmB"
                     /locus_tag="ACF3T5_001580"
     CDS             6144..6809
                     /gene="pgmB"
                     /locus_tag="ACF3T5_001580"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFD3632185.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDRTFNEQLK
                     GVSREDSLSLLLAHGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6802..7809
                     /locus_tag="ACF3T5_001581"
     CDS             6802..7809
                     /locus_tag="ACF3T5_001581"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFD3632186.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEELPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRKMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7831..8850
                     /locus_tag="ACF3T5_001582"
     CDS             7831..8850
                     /locus_tag="ACF3T5_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3632187.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(9131..10498)
                     /gene="nhaC"
                     /locus_tag="ACF3T5_001583"
     CDS             complement(9131..10498)
                     /gene="nhaC"
                     /locus_tag="ACF3T5_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFD3632188.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(10607..12028)
                     /gene="tyrP"
                     /locus_tag="ACF3T5_001584"
     CDS             complement(10607..12028)
                     /gene="tyrP"
                     /locus_tag="ACF3T5_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFD3632189.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(12271..14148)
                     /gene="tdc"
                     /locus_tag="ACF3T5_001585"
     CDS             complement(12271..14148)
                     /gene="tdc"
                     /locus_tag="ACF3T5_001585"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFD3632190.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FTDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(14439..15695)
                     /gene="tyrS"
                     /locus_tag="ACF3T5_001586"
     CDS             complement(14439..15695)
                     /gene="tyrS"
                     /locus_tag="ACF3T5_001586"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFD3632191.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(15807..16012)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4872 a         3468 c         2940 g         4892 t
ORIGIN      
        1 tggagagttt tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac
       61 atctaattcg atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa
      121 aatccgttga tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa
      181 tttttctgta ttttgttccc aaccatcgat tttcttttcc acagtagctg cttttccttc
      241 ttcattcgcg acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata
      301 accttgagag tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc
      361 caaaaagtag ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca
      421 ccaagaaaag tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat
      481 caagatcttg cgtgtctgat aatcttcttt cttttgagat tgggagacgg taaggacctg
      541 actttccaat gtttttttcg caagcagtgc atttccttct tcatatttat cataaccgat
      601 gtggctcatt tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa
      661 acggtaggca aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat
      721 tagcttcgca ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat
      781 cacttcataa tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc
      841 tggctcaagc cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt
      901 catttcgaaa caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt
      961 tctcctgcta actcctcagt aataagctca atggaccagg agctattttt tgtttggctg
     1021 tttttatcac ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac
     1081 tgctgcttct cggtaataag ctcactagcc tagaaaatat gaaaagctat tttctgacta
     1141 gcgctcttat tactcaatgc ataacgcagt tttcacaacc tcttccttaa acggtgttca
     1201 aaagcttgca tctgcggtaa taagcccaac caaacgaaaa ataggaggag ctatttttgt
     1261 ttggctgttc ttattacttg atgcaggaca gctttttcac aacctctcga taaatggtgt
     1321 tcaatcagct gaccttaggt attagttcaa aaaacggcaa aatacgagga gctgtttttt
     1381 cgttttgttt ctctaatact caggtcagga caatgatttc acaacctctc cttacatttt
     1441 aatggagccg atcactttac cagctgtcac tgtacctaat tgcttgatca ctacttcttc
     1501 tactttatcg tttgtaaata cgatcatgat cgttggatct ttttcttcat tttggatcac
     1561 ctcaagatcc atctcagcta atttcgttcc tgttcctact tcttggcctt ctttgacagc
     1621 aatctcaaaa gctggctgat tcatttggac tgtatcgata cccatatgga tcaaaacttc
     1681 gataccatct ttcgtctcca gcccaattgc atgtttgctt gggaaaatac ttgtcacttt
     1741 tccagcgatc ggcgcaacaa cttctcttga tgttggaata acagcaaagc cgtcacccat
     1801 cattttttgt gagaataccg gatcttttac ttcagataaa ggaatcactt ctccagaagc
     1861 tgcggaaact agttcttgtt ctatacctaa gtattgctta ctatctgctt gtctatttcc
     1921 tgtagaaacc atttctggct ctggaatctc tgctcctgat tgcagcagat cttcgatatc
     1981 tgatttcaag acatctgctt ttggtccata aactgcttgg actccattgt ctttgacgat
     2041 caatcccatc gcccctgctc gtttccaggc ttcttcagtc cctacttttt ctcgatcatt
     2101 tacacttacg cgtaaccgag tcatacaagc atcgacatct ttgatatttt cttttcctcc
     2161 cagcaaatga atgatctgga ccacttgctg atctgctgtt cctgttccgc tgttagatac
     2221 aacggtttct tcactgtcat tgtcgtaatt tccgttacgt cccggtgtag caaagttgaa
     2281 ttttttgatc aagaaatttg ctaagaaata cgtaacaaca ccaaaagcga tcacacacaa
     2341 cacgaagttg ataagatctc cacctaaacc cgctttgata gctaacggtg tccgagtcag
     2401 caattcgata ttcccaaatg aatggacccg caatggcaag atatctgcca tcgcaaatgc
     2461 agccccttgg atcactgaat aaatgacata taatggcaca gcagcaaaca taaacatgaa
     2521 ctccaatggt tctgttacac ctgttagaaa gacagccaac gctgctgaaa aatacattga
     2581 tttatatctt gctttcttat caggatcgac attacgatac atcgcaaaag ccattcccat
     2641 caagatacct gaagaaccaa tcatttgtcc tactttgaaa cgagccggcg tccagttatg
     2701 caacacaaac tcatattgcg acatatcccc tgcaccttta agattcacta aatcagtggc
     2761 ccaagccaac cataacggat cttgcccaaa tacttgtgtc cccgcctgtg cgcctgataa
     2821 aatctcatac gatccgccaa gctgtgtata gttgatagga atcgtcagca tatgatgcaa
     2881 tccaaatggc aatagtaaac gttctaatgt tccaaataaa aacggtgcta gaatcggtgc
     2941 gctttcttgt gattctgcga tccataaacc aaaattattg attccagcct gtacgtttgg
     3001 ccacaccaca gcaagaatca atgccacgat tgttgaccat aagataacaa caaacggaac
     3061 aaagcgcttt ccattgaaaa atgacaaagc atccggcaat tttctataat tatagtactt
     3121 attataagcc attgccccga caaatccagc gatgatccct acgaatactc ccatattcaa
     3181 agctggtgct tccaatacac ttgtaaaaaa tcctttaacc atgatcttcg taccgaacag
     3241 agtgtgggta aatgcatctt cattcgcaag catgtcactt gtgacaccaa agatagcccc
     3301 agtgatccga ttgatcaaaa taaatgagat cccagctgca aatgctccgc ctgctctttc
     3361 ttttgcccaa cttcctccta ttgctaaagc aaataaaata tgaagattac caataattgc
     3421 ccaaccaata ttttcaatta ctccgcctgt tgtgacaagc ggcgtccaat caggattgat
     3481 caagggaatt gatttcccta tactgatcat caacccagct gccggcataa ctgctaccac
     3541 aaccattaaa gcctttccaa atttttgcca aaactcaaaa ctaaatatct ttttcattcc
     3601 gtttagctcc tttacgcaat cggtttcgtt aaacgtatta tagcgtgcgt tttctttttt
     3661 gtaaatgctt tattttttat ttttctgtct ataccattct agaaaaccta taatgcgtgt
     3721 gttcccttct ataattttct cctaaaaatt tattttttta tgcttttttc tatgaaatat
     3781 tttcttttgt ctattgactt gtgcaatcgg ttgcgtttat tatgaacgta taaaacagct
     3841 cattaggagg agaaaaatga aacagatcaa acgactattt gctattgatc catggaaaat
     3901 cagcacgaca caattagata aaaaaaatct tcgactgcaa gaatctttaa caagtattgg
     3961 aaacggttat atggggatgc gcgggaactt tgaagaaact tactcagggg accaccatca
     4021 aggtacttat ttggcgggtg tctggtatcc cgataagaca cgtgtcggct ggtggaaaaa
     4081 tggttatcca gaatactttg gaaaagtgat caacgccttg aactttatcg cgatagatat
     4141 ttcgatcaac gatcagctga tcgacttggc ttctttggaa ccagaagact tttactggga
     4201 attagatatg aaaaatggtg tattatctcg tacattcaca atcactactt ctactaataa
     4261 agtacgcatt tccttcgaac gttttctgag cattgtcaaa aaagaatcag cttatatcta
     4321 tttaactata gaaatgcttg aaggtagcgg aacagtcaaa ctcgtatcaa aattggatgg
     4381 aaatgttcaa aacgaagaca gcaactatga tgaacatttt tggaatgaga ttgatcgtgg
     4441 aactatagaa gaaatcggct atttgacgac taaaactatt ccaaacaact ttgggattga
     4501 acaatttact gttactgcag caatgcgcca tttcgttaat ggaaaatcag tcatccctga
     4561 ctatcacgaa gatactttag cattacatgg ttgcttgtct gcggaattat ctcaaggtga
     4621 gacatggtca gtggaaaaag aaattatcgt tcttactagt agagatattc cagaagcgca
     4681 gcaagcagaa aaagctgtct cagaattaac gaaccagaca aacaattata tccaagcaaa
     4741 agaagagcaa acagcggctt gggcagaacg atggaagctt gccgatgtgg tcatcgaagg
     4801 agacgaagaa gcacaacaag gtattcgatt caatttgttc caactattct ctacttacta
     4861 cggcgaagat gaacgattga atatcggccc caaaggattc acaggagaaa aatacggcgg
     4921 agccacttac tgggatacag aagcgtatgc tgtccctctt tatcttgctt tagccaaacc
     4981 tgaagtaact aagaatctgc tgaaatatcg gcataatcaa ctgccgcaag caatccacaa
     5041 cgctcagcaa caaggattaa aaggcgcgct ttatcctatg gtcaccttcg ctggtgtcga
     5101 atgccataac gagtgggaaa tcacattcga ggagattcac cgaaatggcg cgattgccta
     5161 tgcaatctac aattatacga attacaccgg cgatacggaa tatttgaaaa atgaaggact
     5221 ggaagtttta gcagaaatag cacgcttctg ggcagaccgt gtccattttt caaaacgagc
     5281 aaataaatac atgatccacg gtgtgaccgg cccaaacgaa tatgagaaca atatcaataa
     5341 caattggtat acaaatacga tcgcagcttg ggtcttgcgt tacacgcgag aaaattattt
     5401 aacttatcaa aaagaagcta ctatcactat cagcgaagaa gaactagcca aatggaaaga
     5461 tatcgcagag aacatgtact tcccagttga cgaagaatta ggaatctttg tccagcatga
     5521 cacattctta gataaagatc tcatgcctgt atccgatatt cccacaagcg atcttcctct
     5581 aaatcagcat tggtcttggg acaaaatcct tagatcttgc ttcatcaagc aggcagacgt
     5641 actacagggg atttatttct tcaatgatac ttttacaaaa gaagaaaaac aaaaaaactt
     5701 tgatttctat gaaccaatga ctgtccatga gtcttccctt tcgccaagta tccattcgat
     5761 tttagcagca gaattaggaa tggaaagcaa agctgtagaa atgtaccagc gaacagctcg
     5821 cttggatttg gataattaca ataacgacac agaagacggc ttgcacatca cttccatgac
     5881 tggtagctgg ttgtcgattg tccaaggatt tgctcaaatg aaaactgccc atgatcagtt
     5941 gagattcgca ccatttctgc caaaaacttg gaaagcttat tctttccata tcaattatcg
     6001 cggacgttta ttgttcatct cagtcacaga tgaacaagtt actttcgagt tattagaagg
     6061 ttctcctcta gaaatttacg tctatgaaca gtcttattct ttagcggatc gtctagaaat
     6121 aacgctaaga aaggaggaag caaatgttta aaggggtatt gtttgattta gacggtgtca
     6181 ttaccgatac tgctgaattc cattatcgtg cttggcgaaa gcttggtcag gaaatcggca
     6241 ttacgatcga tcgtacattc aacgaacagc taaaaggcgt cagccgagaa gactcactct
     6301 ctttgctttt agctcatggc ggaaaagaac actctttttc aaaggaagaa tttgcagaat
     6361 tagcgaaaag aaaaaacgat tattatttag agatgatcca gacgatcgaa ccaaaagatg
     6421 tcttccccgg agtcgtgcct ttactagatt ctttaaaaga agaaaaaatc aagattgctc
     6481 ttgcttctgc aagtaaaaac gggcctttcc tacttgaaaa aatgggtctt acaccttact
     6541 ttgatgctat tgccgacccg gcagaagcaa caaacggaaa acctgcgcca gatatcttcc
     6601 tccttgcagc aaaagctgtc ggactaaaag cagaagaatg tatcgggatc gaagacgcgc
     6661 aagccggtat ccaagccatc ctctcaagtg gcgctcaacc aattggcgtg ggacgtgctg
     6721 aagaccttgg agaggagatc ccgcttgtcc ctgatacgac ttttcttacg atcgattact
     6781 tgaaagaaaa gtggcatgac catggataat ccagaaatct ccgtcgtacc agttccagag
     6841 actgaaataa agaaaatcac attgaagaac gactacttag aagtcgttct tctcaattac
     6901 ggtgcccgcc tgcaccaaat cttcgcacca gacaaagaag gaaaatcaga aaatgtttta
     6961 ttgtcttacg atcgtttcga agatgttcta acagacaaaa gcttttttgg agcaactgtc
     7021 ggacctgtag ccggaaggat ccgggatgct tcttggggtg agcatagatt ggagaaaaac
     7081 tgcggatcac accatatcca cggtggcacg aaaggctggt ctttccaatt ttgggaagta
     7141 gaagttttca aaactccatc atcaataggt gtggtctttt acttaaaaga tgaattttct
     7201 acctatccag gaccaatcac tgctacgatc acttaccgtt taacaaaaaa cgaattagag
     7261 atgacaacct ctgctagtag ccttgttgaa accatctgca atccgaccaa ccatgcctac
     7321 ttcaaccttt caggaaatgg aacaagagac atctatgaac atcagttaac ggtcttttta
     7381 gatggtgtgc ttgaattaga tcaagaaaaa ctgcctactg gaaattggac aaaaaaagaa
     7441 gaactgccaa tcgactttag aaaatcccca acactgcaag agatccttgc atgttatcca
     7501 gatggactag atgatgtatt tcttttgcat catcctaggc tttccaaaac ttccttacaa
     7561 ttattcgaga agcattctgg tcgcaaaatg actatagcaa cttctaataa aagtatggta
     7621 ttattttcta cgactggatt cgaagctgat ttctctgtca atggaaaaca gatgcattca
     7681 aactacggat tagcaatcga accgcaagaa atccctgata tcgttcattt ccccaaattt
     7741 ggatcgatca acctccatcc tggtcaagaa cggatcagtc agacgattta tcggttctct
     7801 gcacaataac aagagaaagg tgtttttatc atggctatta ccgtaaaaga tgtggcgaaa
     7861 aaagccggtg ttgccacctc gaccgtctct cgtgtcatca acgatcatcc tagtatttcc
     7921 gagacaacaa agaagaaagt ccgaaaaatt atggatgaac taggttatgt tcctaacatg
     7981 actgcccgca atctaggaaa acgcatttcc agtgccatcg gcgtaatctt accccctctt
     8041 gattcaaaag agcggatcgg taacccattt tatcttgaga tcatggaagc aatcaacgaa
     8101 gaagcgcgga attatgatat gactacagca atcgctactg ctaaaaattt tgaattgctg
     8161 ttagagaacg tccagcgtat gcatttacaa aaacaagtag atggttttat tctagtgtat
     8221 tcagatagtc aagatcctgt cattgattat ttataccaaa accacatccc tttcactttg
     8281 atcggacagc cttatcatca tgagaacgaa atcgtttatg tagataatga taaccagctt
     8341 ctaggtaaac aagcaacaga atttttgatc cagaatggac ataagaatat cctgtttgtg
     8401 acaaacacga ctcatgaaag tttgtatttc gaacgatatt tcggttatca aaaagcaatg
     8461 atgatggctg gtcttcctgc gttatccagt gtagtactag agcatccgga agattatctg
     8521 tcctttgaga aaacgttgaa agaaaacgaa gccacagcat tggtggtcat tgacgatatc
     8581 ttcgcccttc gcacgatgca gttggcgcaa atgtacggct atgtcattcc agagacactc
     8641 tccgtcatta gcttcaacaa ctcgatcttc tcaacattaa cacaccctta ccttacaagt
     8701 atcgatatcg atatctccga attaggacgt gtcgctatgc aaaagctgcg cgaattgatc
     8761 catgaagaat tgagcagtgg cgtacgtttg gtcatccctc atcgcttgat caaaagagag
     8821 acagtcgttc ctttagaaaa cccatactaa atagctgact cactaaatac gctgaccatt
     8881 tatcctatgg tcagcttttt atgtaaaaga agatactact tctaacacat tgtttatctg
     8941 taaaaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta aacacgtata
     9001 aacggcggaa gcagaagcaa ctccttcgga aataagctga aattcacaaa aatttaaaaa
     9061 gcaattttct tgagtttctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt
     9121 tttccttcct tcaatttttt acacgtcgac gattcaataa aaaaccgcct aatattgtaa
     9181 agacaggtgc aagaatcgag aaaaaggcga atggcaagta atgcaacgcg ctaactttca
     9241 tcgttcccat aatgaatgtg ccgctgactc cccacggtac gagagaattg acggtcgcac
     9301 ctgaatccgc tagtgttcgt gtcaaatagg tttttgataa gccaacgtca tcaaaagaac
     9361 gcttgaatgt ttctcctgga agaatgatgg acagatactg ttctccaacg atcagattga
     9421 ttccgataca gctcaaggca gtaaacccga ctaaacgaga aggtcggtcc atcttttctt
     9481 ttaattcttg aacgatggta tcaacgatca gatatttgat cagtaaccca cctaatgcca
     9541 atgcaagtaa aatcaaacta gcagaaccta acatactcat gatcccgcct cgagaaagca
     9601 gttcatctag ctttggatca ggtgtatttg cgacataacc attcatcaag atgtctgctg
     9661 cttttccgac cgtcaaactt ttctcatgga taaaagaaag aacgagagca gctgtcgatc
     9721 ccgctagtaa tgtagggata gccggaatct ttttgattgc ccccccaaat aaaataacta
     9781 gtggaatcaa tgtccaagga gagatccaaa agctcgcttt catgttttgg atcatttcat
     9841 tgatactagc tagatcggct gaatgactgt tatgtcctaa tatcgtataa aaaatcaacg
     9901 aaaggacgaa tgctgggaaa tccgtataac gcataccagc gatatgttcg aatagattaa
     9961 ctcctccaat acccgctgcc aagttggtcg tatctgataa aggcgagcag ttattcccta
    10021 aaaaggcacc ggaaacgact gcgcctgtag tcattgcatc attgaatcca agtaaatgac
    10081 cgatccccaa aaaagcaatt ccaatcgtcg aaatcgttgt aaatgaactg ccgacagtca
    10141 ctccgacaat tgcacaaaca agaaatgctg tcggtaaaaa gaaacgcaca gaaacaagtt
    10201 taaatccata aaccataatc gtagggatgg ttccagataa aatccatgaa gcaactaaga
    10261 caccaatcaa aagaaaaatc aggattggaa tgatccctgg agtgattcct ttgatgattc
    10321 cgtcatggat ctcatcccat gtgaactttt taaatctgcc ataaaacaac aaacacatga
    10381 aagctgcaag gatcgggata tgtggcgaga gctggaatcc gatgatcagt actcctagga
    10441 gtgccaacaa cacgatcaat acagcgatac tttcttttaa agaaatattt ttattcatcg
    10501 tcgatctcct atttcaaatg aaaaggccct gccatgaaaa aactattcac ttggcaggac
    10561 ctcctactaa atcagcgaca aaacgtcggc tgaaagactt atttttttat ttttcttcta
    10621 aacttttttc tacctcaatc ccccaagaat ctttcttgaa gtgattgatg atcaatggaa
    10681 taacgaacat gaccactgtg atagctgcta ctaataatgc atcactcaat gtcgctgtac
    10741 ctaatgtagc aaatacgaca acgatgatag agaagatcag catgcagctg actacccaag
    10801 ctaatccgtt tccgttttta ccaattcggt aaggacgttc gaattctgga tgtttcttac
    10861 gcattttaag caatgaaaca gcaatcaaaa tgtacacgat acaataaata acagttgtag
    10921 catttgtaag tgtcaagaat acagcactta cgtcatccat tacaccataa agcaaagcaa
    10981 ataatgaaat aacgattgat tgtgttagaa cgacatttct agatacgccg tatttgtttt
    11041 ctttatggaa tccgaatttt ggtggaagga atccttcacg agctacttgg atgattgttt
    11101 ttgatggtcc agtaacccaa gctgataatt gtaaaagcac accaataaat accatgaagc
    11161 tgaagatatt tccgatcact tctggtaagc ctaggatttg acagtcaatc aagataggct
    11221 gcgtgatatt tgctaattcc atttttccat ctggtacgac atttgctaca agcatcgcat
    11281 tgatgacatt caataaaact aatccgatca aagcgataaa tacgcctttt gtataatttt
    11341 ttgttgcgtc tttcaaacga ggcatgtaaa cagaactcat ttcgatccca acaaaaataa
    11401 atgtgatacc agctaagtat ttcaatgaat caatatgttc caagtcaggg aaagcttttg
    11461 accatgaaaa gtcacctaga tagccgtttg agactagtcc gactttgaag gctgccaaaa
    11521 cacctaaaac gaacatgatg actactggga tatatacccc tagccataca ccgatgttcc
    11581 cgccgacttt tgccatatcg aatttcaagt tcaagatcgt gatgatccag tagataacta
    11641 aaatacaacc taaaacgaac cagtgattat tgccaagttc tacattacca aatgtgtttc
    11701 ctaacaatgg tcctaaagta gaagcgacca ttaccatacc tgggaacatt tgcacccata
    11761 atagccatgc aacgacaaat ccccatttgc tgcccagtgc tgtttttacc cataactgag
    11821 gaccgccttc ttgaggaagc atcgttgaaa gctcaccgga aaccattgat atcggaccgg
    11881 caaaaaacaa aactgcaaaa attgaataaa aaatcaatgt ccaaccgact gcggctaacg
    11941 taggaatact ccgaaccgtt gcacacagtg ccattgtcag accgataaag gtcccaagac
    12001 ccattttact tacttcagat ttactcaccg ttgacactct ccttgatttt ttggataaaa
    12061 taacgatgtc tttgaagtag tttcagcaat aaacgaaaca attcagcgaa tatttgaggg
    12121 gattctttga gtctattgct tgaagggttt cttcaaagac aacctcaaaa caaagggatc
    12181 gaagagctta ggtctcccct tgctcttcag atccaaacat gcgtcagaaa cagttagtat
    12241 tcaagctgtt ttctaacatg attaccttgc ttattttgct tcgcttgcca ataattggtc
    12301 tgcgtagatt ttttccaatt tttcttgaag agctgctttg attttttcag catattcttc
    12361 aaagtgttct tctttgttca tgtatggtgt cattacgctg gcacgcaata cagttacttt
    12421 tccggcacgg ttccattctt catctgtgaa tcctagttgg ttcacaaatt gtaatgggct
    12481 gttgccatag tctgggatag caaagtcagt atgtgaagtt aagaactcat taccgtaaat
    12541 gcttcctttc acgtatgaag aataatcata tacgtcatgg ttcaatttat tcattgcaac
    12601 taagtcatcg ttgccttttt ctttgaatac gtagtctacc atattgaaat ctggataagt
    12661 taatggatga acttcgattt ctttgtctcc tactttaaat gacaagtcat tcaagaagtt
    12721 gtagaaacga tgtgcacctt cgattgatgc acccattaat ttaccatatc ctgtgacatt
    12781 caaaggtaag acatggtgtg ctgcccaaac gctggctgct gttgcccctg cttttgaacc
    12841 ttccaagata taagccccaa gtaatgctgg gatatctgcg cctttttcaa atacatatgt
    12901 cgcaaagtaa gagataacgt ctctcatgcg gatatctttg atgacgatac caccggcaga
    12961 atatggaaca tagcccattt tatgtgggtc aatcgttaca gattctgctt cttcgattgc
    13021 tttatatgca ctgtgtactt cttctaagat ataatctttg ttttctgtaa agacattgta
    13081 tttgtaatga acatctttta aatcttcaaa tggaatgaag ttgttgtctt cgtctaagaa
    13141 aatcgcacgg ccgtatccgc cataagcagc atctacgtgt aagtagaagt aaataccgtc
    13201 tttttctaat acgcgacgca atgcaacgat tttatcgata ccgtcgattg ctccttcttc
    13261 tgttgatcct acaacaccga ctacacctag gattggtgtt ttttcagctg ctagaccacg
    13321 aacgattttt tcaagttcgt tgatgtccat gcgatagttg tggtcaactg gcactggaat
    13381 cacttggtct aaaccgatac cgatgatgtc ggcagctttt agccatgaat agtgtttagt
    13441 ttgtggaacc aaccattttc ctaatttttc taaatgtttt ccgctacgtg ctgagtgagc
    13501 tttgatttcg tcgatttttt ctgggacgct gtctaacaga ttcatgattt cttctgttga
    13561 caagttcatt aattcccaat cgctttttcc agcaactagt tcaggtgtca cttctttcat
    13621 tgcaagcggc aatgatttga tgttgcgggc ataccaaagt ccttctaagt ttgctaaaga
    13681 accatcagca acgatgtggc cccagccatc tttatagctc attaattttg caaattccat
    13741 tcctacttct tcttccattt ggcttgttgc aggagaagat tcataggcaa cgttattacc
    13801 attccatagc atagcaaaat tgtacgctag taaagatggc atcaatgttt cagagttcat
    13861 atgaccccag taacgtccgg cattatgcca aggaacggaa tgggtacgca tccgcgctga
    13921 aatttctgat agaacatcct ttgttttatt cacagtgtgt tcaaaactag cactgctttt
    13981 ttcttctggc gtaataatag gcatgtcttg aggcatgtag ttttgacgcc agcctaaatg
    14041 ttcatccacc aattcattca acaaagcttt atagatttgg ccgttctcag ctttgtcccc
    14101 aatgaatagg gcatttaggt ttagatcttt cgacaatgat tcactcatag tgaaaccctc
    14161 ctaaaaaaat atttaaattt tgtcgagccc tacattacac ctgttcttct attctgtaaa
    14221 ctaagttttg gcctgatttt taatcatatt ttaatatgta tcaattatcc gaattgttca
    14281 cacttttgaa ataattaaaa aaaacgaggg aatttacaaa tgtaaactcc ctcgcaaaga
    14341 ttgtattaac gacaaccagc gttctatttt aggatgggtt tcaaaattgg aatgttgtaa
    14401 tagtatttcc ctttttcaag gtaaaaactt actaaattct aatcaattac tttggccaaa
    14461 aagtaatttt tcttaccttt tcgaataacc acaaacttac catcgaattc atcggatgga
    14521 tttatgacaa aatttaaatc ggtaacgcgg tcaccgttaa tacttatagc tccgtttgaa
    14581 acatcttcac gtgcttgacg tttagatggt tcaatttttg tggaaacaag cagttccacg
    14641 atattttcag gtgtgcttga aatttcaacg ttcggcattt taccgaatcc ttgcgcgatt
    14701 tcttccgcat ttagttcttt aatatttcca gagaacaatg cttgcgttat tttttgcgct
    14761 tctttcaagt cttcttcact atgcacaaat cttgtaactt cttctgccaa acggcgttgt
    14821 gcttcacgtt tctcaggttc tgtctctact tttttcgcta agtcttcgat ttcttcttga
    14881 gacaagaaag taaagaattt caagtatttc actacatcgc gatcatcttg attcaaccag
    14941 aattggtaga actcaaatgg tgaagttttc ttcggatcaa gccaaacggc accgcctgca
    15001 gtttttccga atttcgttcc gtcagccttc agcatcaatg ggatcgttaa accgaacgct
    15061 tctacttctg gtccttctaa ttttcggatt aggtctaatc cagctgtgat atttccccat
    15121 tggtctgctc caccgatttg caattgcaca tcgtatgttt tatgcagatg caaaaagtcg
    15181 atagattgca gaatttggta agtaaactct gtaaatgaga ttcctacttc caaacggctg
    15241 gcaacgatat cttttgctaa catcgtgttg atgttgaagt ttttaccgta gtctctcaaa
    15301 aattcaagta aagagatttt tgacaaccag tcgtagttgt tcacaaatgt gatatttgca
    15361 tctttaccaa acaattttct catttgattg gaaagtgcgt ccacgttatg ttgcacttgt
    15421 tccatcgttt gcaacacacg ttctgttttg cggccgcttg ggtcgccgat cgttcctgtt
    15481 ccgccgccaa tcagaatata tggatgatgg cctgccaatt ggaatctttt catcatcata
    15541 aatggaatca aatgtccgat atgcatcgaa tcaccagttg gatcaacgcc gcaataaagt
    15601 gaaatgctct tttcttcaac gagctcgcga agtctttctt cgtttgtttg ctgattaatg
    15661 gcgtcgcgcc aagttaattc atcaataata ttcattttac tgacctccgt gtagtttttt
    15721 tatgaaagca ggaacctgct agcatgtgta attaattatt tcttcaggat aggataggaa
    15781 ataaaaaaag tcccttggca tataagccaa gggacgaaat ctcgcggtac cacccaattt
    15841 gtatgtctag atatccaaca tacctctcga acatttaacg ctgtcgcgtg gcaatcactg
    15901 ccaaagactc ctgaaatgta attcgcttta aagcatgtac tggtttgcag cgaccaccag
    15961 ctttctgaaa cattggcttt aacaactact tcgtttcgat cttcgtcttg ttattattaa
    16021 tatgtgtcta tcatagagcg caactttttc tttgtcaaca gaaaaaggat atttttttta
    16081 aattcctcat tttttctttt ttgaatccgc tctaattccg aacattttat tttcaattat
    16141 tgttaaaaga aataactttt tctgtatttt tt
//
LOCUS       DAXLPI010000056        16046 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXLPI010000056 DAXLPI010000000
VERSION     DAXLPI010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.056"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(283..585)
                     /locus_tag="ACF3T5_001587"
     CDS             complement(283..585)
                     /locus_tag="ACF3T5_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFD3632192.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(805..1171)
                     /gene="ssrA"
                     /locus_tag="ACF3T5_001588"
     tmRNA           complement(805..1171)
                     /gene="ssrA"
                     /locus_tag="ACF3T5_001588"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1342..2115)
                     /locus_tag="ACF3T5_001589"
     CDS             complement(1342..2115)
                     /locus_tag="ACF3T5_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFD3632193.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2259..2609
                     /locus_tag="ACF3T5_001590"
     CDS             2259..2609
                     /locus_tag="ACF3T5_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFD3632194.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2590..3306
                     /locus_tag="ACF3T5_001591"
     CDS             2590..3306
                     /locus_tag="ACF3T5_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFD3632195.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3306..4754
                     /locus_tag="ACF3T5_001592"
     CDS             3306..4754
                     /locus_tag="ACF3T5_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD3632196.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4824..5333)
                     /locus_tag="ACF3T5_001593"
     CDS             complement(4824..5333)
                     /locus_tag="ACF3T5_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632197.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5323..6047)
                     /locus_tag="ACF3T5_001594"
                     /pseudo
     CDS             complement(5323..6047)
                     /locus_tag="ACF3T5_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6270..8390)
                     /locus_tag="ACF3T5_001595"
     CDS             complement(6270..8390)
                     /locus_tag="ACF3T5_001595"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFD3632198.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8620..9798
                     /locus_tag="ACF3T5_001596"
     CDS             8620..9798
                     /locus_tag="ACF3T5_001596"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFD3632199.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9814..10572
                     /gene="aroD"
                     /locus_tag="ACF3T5_001597"
     CDS             9814..10572
                     /gene="aroD"
                     /locus_tag="ACF3T5_001597"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFD3632200.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10595..11080
                     /gene="ybaK"
                     /locus_tag="ACF3T5_001598"
     CDS             10595..11080
                     /gene="ybaK"
                     /locus_tag="ACF3T5_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFD3632201.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11151..12404)
                     /locus_tag="ACF3T5_001599"
     CDS             complement(11151..12404)
                     /locus_tag="ACF3T5_001599"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFD3632202.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12521..13870
                     /gene="gdhA"
                     /locus_tag="ACF3T5_001600"
     CDS             12521..13870
                     /gene="gdhA"
                     /locus_tag="ACF3T5_001600"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFD3632203.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13982..15328
                     /locus_tag="ACF3T5_001601"
     CDS             13982..15328
                     /locus_tag="ACF3T5_001601"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFD3632204.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15636..16022)
                     /locus_tag="ACF3T5_001602"
     CDS             complement(15636..16022)
                     /locus_tag="ACF3T5_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFD3632205.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5258 a         2867 c         3206 g         4715 t
ORIGIN      
        1 taaagcgcac cccaaaccac ttccccataa gtgtgttacg ctttaaactc ttttatattt
       61 gaagccatta aaaagcatac catatttttg aaaaaaagtg agaaaaaagg cttacaattg
      121 gagtggtagt taattagtga cttatttttg attttatagc actgatacta taaaatatag
      181 atatcatcat attacacaat cttaatacta acttaaaaaa tatccctttt ataagtatgg
      241 tgataaaatc cgttccgggc tacctttttt aggtagccta ctttaatctt tatacctttc
      301 tggatcaacg aaagtatact tcaaatagtc ataacgccga tgatcgctcc gtgcgtctgg
      361 cacatcagtc acgatatcaa acaaaaaata cacatctttc ttcattctgg tttttgttgc
      421 cggaatttta aagtagttct tattagagta atagagattg attaataagc tatcttcgat
      481 tgctaaaaag aaaacttctg aatcccacac cttataaaaa tctttgacaa atctattcga
      541 aggatcaaat ttaaaccata attgtgtttt tccttccatc aacataatgt tcacctcaaa
      601 aagagtatac gaactaatgt tctttttgta aagtaggaat atcattgata atatggatgg
      661 aacatatata ttaaaaagtg ttctttttga atgctatttt gaatgccatt ttcattcatt
      721 taacgaaaat ctgcgaaaat aaaaaatgct caaaccctta taaaataagg atttaagcat
      781 ctagcgaaat ctgctgaaat ctcacaatgg agacggcggg agtcgaaccc gcgtccaaac
      841 acatcgccac ttaaaaatct acgttcatag tcaactcatt taaggattca ctttacagct
      901 tgccgagtga caggcattct gtatcgctag tctgatgttc tctttttcaa cttacagacg
      961 gaaagttgaa acgtatccca ctaaagttag gacccttact cgagcacatg ggcgatgccg
     1021 agaggatcta cgctaactgt ttttaggcag ctaaagcgta agagttgttt tcgtttttag
     1081 cagttatatt taactgtaac gtttttacgt agacgtaacc tacgaaacgc aattcaagct
     1141 cgactgtgcc tgtcgaatcc gtaacgtccc cgttagctaa caagggttac gtgtattttt
     1201 catacaaaat tagtatagca tatattggga taaattcaat aaaaaagcga atcatatccc
     1261 attatttata taataactgc ccctttctta agaaaaaata agcgattttc ttaagaaaag
     1321 aggtagcttc aaaaaaagat cttaaatcag atcaaaatac ttcaatgcgt ggacaattcc
     1381 gccttctgta tttttctttg tgataaaggt agaaagttct tttaatccgt cataggcgtt
     1441 gcccatagcg attttgttgt cgcaagcttc caacaaggca aaatcattcg gaccatcgcc
     1501 aaatccaaaa gtaggaactt tttccagctg aagttctttt tgcagacgtt tgacacctgc
     1561 cccttttgag attcctttct tcactgtatc gatcgagtaa ggaccattcc gataaaaagt
     1621 tagctcagga aaatgttcgt gatagtattc gtcacctgat tgagataaga tcagcaacat
     1681 atttactgtg ttttctttaa agcctaaagg atcaatttcc ggaacatctg aatgaatgaa
     1741 gtcgtaagca ttgatcattg tttggctatg accggtacac cagattttct gatcgttata
     1801 gtaagaaagt tcatgaccgt tcaactgtac atgatcatac attttttgac attcttctga
     1861 agaaaatgtt tgagaataga tttcttcgcc gtctactcga atgaaagaac cattcatggt
     1921 gaccgcagaa gtaatgcccg catcttccat gatagagtgg atttctgctt ctgtacgtcc
     1981 tgtagcgatg acggggagaa cattgttttc ttttaatgcg ttcatagctt ttgccacttc
     2041 gggtgtgatt tttgatttcg catctagtaa tgttccatca agatcaaaga aagtgattgc
     2101 acgataattt gacacaaata aaactccttt tttattggaa taagtttatc ttaacaaaga
     2161 atgaacaaaa gacaaacctt tcgtgaagaa ttcctgaaca tattggttca gtcaacaatt
     2221 tagagtatac tataagaaga gaatttagga gggaaagaat gatttttttc agtattggta
     2281 tcttattctt aataattggc agtctttttc ggattctgcc ctcaaaagga aatctgcctt
     2341 tttatgggta tcattcgcct cttgccgcaa aaacagacgc tcattggcgg ctggctcaaa
     2401 agacaagcgg aaactggttt tttcttatgg gcttactgat ggcattgatc ggctattatt
     2461 taaagacatc tgggcatacg aattattttc tgattgaaat gcttctgcta gtatttccaa
     2521 ttatgcctat ttttattatg acagaaaaga aattacagaa atatgacctg gaaactggag
     2581 ggaacgataa tgaatatttt aatgattgaa gataatcaat ctgtttctga aatgatgcag
     2641 atgtttttct taaatgaagg ttgggaagca actttcaagt atgatggaaa agaagggctc
     2701 gatgcctttt tagaaaaccc acaacattgg gatatgatta cactagacct gaatttgccc
     2761 acactggacg gtatgctagt aggaagagag atccgcaaag tttcaagtac tgtgccgatt
     2821 atcatgctga cagctagaga ttctgaaagc gatcaggtga tcggcttaga gatgggggca
     2881 gatgattatg tcacaaaacc tttcagtcct ctgacgctta ttgctcgaat gaaagctttg
     2941 catcgccgta gtgagttggc agaagcaaga gaagaagcag tagtttcaga tgatcatttt
     3001 gatatcgaga ctagtcattt caagatgaat acgaaaacac gagaagcgta tctggacaac
     3061 cggccaattg aaggactgac gcctaaagag ttcgatctat tgtttacttt agcaaaaaaa
     3121 ccacgtcaag tattctctcg ggagcagttg cttgaattag tctgggatta tcagtacttt
     3181 ggtgatgaac ggacagtgga cgcccatatc aagaaattac gacaaaaaat cgaaaaggtc
     3241 ggaccacaag tcatccaaac tgtttgggga gtcggctaca aattcgatga ttctggtgtt
     3301 gcctaatgcg ttatttgtat cagcaactct tagctttctg cggaatggtc gcgttgatct
     3361 tattgatcgt tggagtttct tttacacaac tgacaaaaca aacgattgaa gaaaacaatt
     3421 accagcaact gtttggatat gctgaatcag ttgaaaagac aacgcaaacc tatgccgata
     3481 cgctcccgca gtttagtcaa gatcaggcgt ttcaaaatgc attgctgctg acagagcaag
     3541 tactgacaga acaaaatgtg aactttatat ttatcgataa aaacgaacgt gtcatttatc
     3601 cgactaatac agctttactg aattttacct taacagctga tcaatggaaa aatctgagga
     3661 atggacgtca agaaaaattc acatcgaata aaaatatttt aggacaagca caggcgacct
     3721 cttacgcact tgtgccattt aatttgaatc atgaattcta tggagcactt gtagttagtc
     3781 aacctgcgcg aaatatcgat aacagcgttc gtccaattac actgaacctg ttcaaaggat
     3841 ttattttttc cagtatcgtt gcagttattg ccagctatgc ttttgcagca ttccaagtta
     3901 agcgtatcaa tcggttaagg aatgcgacga aagaagtgac gaatggtaat tttgatgtac
     3961 agctccccgt acatgacaaa gatgaatttg atgagttagc agatgatttc aataaaatga
     4021 cgaattcatt gaaagaatct caagcagaaa ttgaagaaca agaaaatcgt cgaagacaat
     4081 ttatggcaga tgcttctcac gagatgagaa cacctcttac gacaatcaac ggacttctgg
     4141 aagggttaga atacaatgca atccctgaaa atcagcgaga aaacgcgatt aaattgatga
     4201 aaaatgaaac agagcgtctg atccgtttgg tgaatgaaaa cttggattat gaaaaaatcc
     4261 gtacgaatca aatcagtatg gtcattaaaa aattcaatgg aacagaaaca ttgaaaaata
     4321 ttgttgcaca gttagaagct aaagcagaag cagcaggaga tacattgaca ttgaaagccg
     4381 ttgatgatat tgatgtttac gctgattatg accgatttgt ccaaatcatg gtcaatatta
     4441 ttcaaaacgc gatacagttt actgaaaacg gccaaatcac gattacttta gaaaaaggct
     4501 atcttgaaac gatcatcacg attgaagata cgggaattgg aatgtctgaa caacaaatga
     4561 aaagtatctg ggatcgttat tataaagtag atccttcaag aaaaaataca aaatatggtg
     4621 aatcaggatt aggtttacca atcgtgcagc aacttgtacg cttgcataaa ggaaaattag
     4681 aagtagaaag cgaactagga aaaggaacaa aattcgctgt tcgtttgcct gacgtagaaa
     4741 tcaaaaaaga gtaaaaaaca agcggtataa aagatcatga atcgatcttc tataccgctt
     4801 gtttttacta taaaatgatt gatttagccc gcgggctcta aacgatcaat ataaggactt
     4861 tgagcaatag tagcatcatg agtaaagagt aagatacctt gattttcttc atgagctaaa
     4921 taagaaagca agtggagtag ctcttggatc tccaatacac taagttcatc aaatgtatct
     4981 tcaataacaa tattgtttcg cttggaaagc aaggcatgaa gtaattgcag ctttactttt
     5041 tcaaaagacg tcaactcagc tgccgcacaa ttaagcaagg aaggagagat atcaagtttt
     5101 tcttgatagg ccaaacgatc ttgtttgatt tctttttccg gtacatccaa caggacatgg
     5161 tctttaagat tcagataagg aaacatcggc caattttttt ggatgatcgc aaaattatcc
     5221 agttttgcaa caagaacctc ttttttcaag tgctccaata ttttatgctg tttttcgaga
     5281 ggacacaaaa taacggtcaa gtgttcagca tgaatcaaat attcaggttt cattacgtcc
     5341 aggcctccta ctttgcaaat gatggataaa aaagtgaaag ggaatagact aagaatgaga
     5401 cagcatccac tgagcccaat aagagtttgg gcaaatccct taagtatatc tgtgaaaaaa
     5461 agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat ccatcggaat tgtaagtccg
     5521 cttaccgaag tcaagatatc acggatatct ggcagttcag tagtctttaa gacaagaaaa
     5581 ttgatccttt gaagaagcgc ttcataaact gactggaagc ttacgatcca caataaaacc
     5641 aagatacaac aaagaaagag aggaaagaac agctgccaga aaataaaaag gaataatttg
     5701 gttttagaaa gaccagtcag gtgccaattg atcaattcct gcttgttttg cctgactgaa
     5761 tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa ataaactgaa tatgcctttt
     5821 atataaaatc ggctagctaa ttctaaagcc ggaagaagct ctgtagatgc agaagcgcca
     5881 ataaatgaaa gccgatcttg gacttgattg gctaaagccg attgcaaatg gtaaagagtc
     5941 aggcatgcta tcaataaaaa agcagaaaag cttgaaaata aacaatagag tattgtctgc
     6001 tttttgtaat aaattaatcg agcagtagca tagcggatta ttttcatact tttcacctct
     6061 ataaaaagta tagaaaatga atatgaataa agtatgaaat tcctttagtg aaatcacgaa
     6121 ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg aagaagctgc cctattctat
     6181 taccgtagat gcaagctttt gaacactgtt tattcgaaaa gaggagcagc gacaataatt
     6241 ttgtcgctgc tccttttaaa tttattttat cattcggaag aacttgtggt cgttccgtga
     6301 agttcttggt acgtttgcgt tttataaaga tcaacagttg attgtccgtt gtgttcacta
     6361 aagacactag ttgacttgtc accaagagat ttttcagctt cttttaattt ctctaagcca
     6421 ttcttgtagt cataatcact ggtatcaatt gggtctaatc cactattcgt atagaaccga
     6481 agaagatcac cattattgat ttggtcagaa acagctaact gtttatcaac tttttctttc
     6541 agtgaatcaa tttcattctg tacttcctca gtaggttcag taatagcttc accagtttcg
     6601 ttactataga tcgtaccacc gtagtaagta tattttggtg taacaaaatc accatcacgg
     6661 aaagaaacaa gctgttcatg atctttcgag aatagatctt gtcctaactg aatataattt
     6721 tttgtatcta cacctagtaa gtgaagtaat gtaggcaagg catcaacttg tccgccgtaa
     6781 gtgtgattga ttccgccctg tgtttgtcca gggatatgga tcatataagg gacacgctgc
     6841 atttgtgcat tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa ttcagctaga
     6901 tttttatttc ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat gactgaatta
     6961 tcatacaagc cgctttcttt caaataattg aagaattctt gtacggcggt atctaaataa
     7021 ttggctgtcg caaagtaacc gttgatcgtt tcatcatttg tattggctaa cgggaatcct
     7081 gcttcgtcat tggtgaattt tgaataagga taatggttag atacagcaat gaattttgaa
     7141 taaaatggtt gttgcagacg ttccaagtat tgtacggatt gttggaaaaa tggtttatca
     7201 tgcaacccgt attggaacga attgtcatca ttaacatcat aataagaaga atcaaagaaa
     7261 taatcatatc ctaaattctt gtatgtctcg tttcgattcc agaatgtacc agcgttccca
     7321 tggaataccg cactaatgta accttgtgtt tgacttaaaa tatcaggtgc agcttggaaa
     7381 gtattttttc cgcctaattg tgtgaacaag gagccttgat tcaagccaaa caatgaattt
     7441 tccatcagcg tttccgcatc gcttgttttt cctgctttta cttgatggaa aaagttgtca
     7501 aaactgaacg tgctgttgct gtggtaaaga ctattgatga aaggtgtaac ttcatgctgg
     7561 agaccattct catctgtcaa tttataatcg atcaggaatt gctggaagct ttctaaatgg
     7621 atataaatca cattttttcc tctagcgagc ccgaacaaat cgtcatttgg tgctgcataa
     7681 tgttctttta cataattttc tacttctttc atatcattgg gacttgcttc tgcccgcact
     7741 tggtttgtct ggtaagtttg aacaccgtca taaaccatga aagcattcaa gccaagatat
     7801 ttgaccaaat aatcacgaga gaatgttcga gtcagcaatt ctggacgatc agcttccgcc
     7861 ataaaaagat ttcctgagaa aatcataact gctaatgtag agatcgcaaa agccattctt
     7921 gctcggatcg ggcgagggtc caacttgatt ttttttgcaa tcagcagaac agtcaagata
     7981 ataaaatcga tccaatagat cacatcatat gggcgaaaca gtcgaatggc actttctccc
     8041 agaccactcg ctactttccc agcacctaaa atcgtgttga tcgtaatgaa gtccgtgaat
     8101 tctcgataat aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata aatgaccatc
     8161 atcgtgatat aagcaactct agggcgtctg atataaagag caattccaag taataacaat
     8221 gtagttgcaa tagggttgat aaacaagata aagtattgaa tgccactttc gattccgaga
     8281 tggaaatcaa ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacgac tagtaaagaa
     8341 aaaaatccga gcctcttatt caaaaaggca ggtgttttta tacatttcaa gataatcttc
     8401 ctttctgcac ttcttaatca cagaagtgtc ctgaaaccat gttacacagc tcacttattc
     8461 tcatttattt tactagtatg acaatatagt gtcaattgaa accagaagtt tatgctgttt
     8521 cattaaggtt ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc ctatgttttt
     8581 tgttatactt gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa agtaaaaaaa
     8641 agagcaagca atcgattgaa aaaaggatat ccactgatcc aaaaagaaga ccttgctcat
     8701 ccccaaaaag aaaccacgca atgggtggaa ttagttgatt cgcaaaacca atttatcgca
     8761 aaaggatatc taggaaagca gaataaggga atcggttggg tattgagcca gcagaacatc
     8821 ccatttgatc aatctttttt tgaagaattt tttgtgaagt caaaagaaaa aagaactttc
     8881 tttttttcag atgattctac taccgctttt cgtatattca atggagaagg agacgggatc
     8941 ggcggtctga ttgttgatta ttatgcaggt tatgctgttt tttcttggta taacgaaacg
     9001 ttatatgcac atcgtgaatt attgattcaa gcttttcgtg aaatctaccc ggaaatcata
     9061 ggaggctatg agaaaattcg atttgaatca gtaaatctgc ctgagagcca gttcatttac
     9121 ggaaaagagg caccggaacc cttgatcgta aaagaaaacg gagtaaaatt tgccgtttat
     9181 ttaaatgaag gattaatgac tgggatcttt ttagaccaaa aagaagtacg gggatcatta
     9241 gcagaaggtt tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac cggcgccttt
     9301 tctgttgctg ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct agcaaaacgc
     9361 agtttaaaaa aaacacagga acagtttgaa gtgaatggtt tggatgtcga tcagcaaaaa
     9421 atcattgtta tggacgtatt cgagtatttc aaatacgcca aaagaaaaca atttttatat
     9481 gacatgattg tattggatcc accaagtttt gccagaaata agaaaaaagt ctttcgcgta
     9541 gcaaaagatt atggggaact ggtcaaggat agtctggata ttttagcaga agacgggata
     9601 cttattgcgt ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac aatgattgaa
     9661 gaggaattcc atgccaaaaa tgtttctttt gaacaacttg ctttatatcg tctgccaaaa
     9721 gatttccaga cgacggatac ctttttagaa ggcaattatc tgaaagtgtt tgtttatcaa
     9781 attaaaaata accattagaa ggagcgatgc agaatggttc gggtaaaggg attgaatata
     9841 ggaagcggta gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga ggaagtatta
     9901 cgtgaagcag ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg agtcgatcat
     9961 tatcgtgaag ttttttcttt tgatgaagta gcagaaacat tggttcttat ccatgagatg
    10021 cttgatggac tgccgttatt atttacgttt cgtaccttag ctgaaggcgg agaacaagac
    10081 atatcgatcc gtcagtatcg cgacctttat gaacatgtga tacacacagg attagtagat
    10141 atggtcgata tcgagttgtt caaagtagaa agtctaggaa aaagactgat agaggaaatc
    10201 aagtctttaa acattccttt aattatcagt agtcatgatt ttaaagaaac accagcagac
    10261 cctgtcttgc tttaccgatt gaatgttatg gaacattttg gagccgatat cggaaaactg
    10321 gcagtaactc ccaataatga acgagatgtt ttgcggttga tggagttgac tagacgggca
    10381 aatgcttttt tctccatgcc tattattacg atgtccatgg gaaatcttgg taaaatttct
    10441 cgtctggttg gcggaacgac gggttccgtt atgacatttg gttccttaag cagcgattct
    10501 acttctgctc caggacaatt accggtcaaa gcattgaagc aaattattca attattaaat
    10561 gaaccgttgt agaacaagtt tgaggtgata ttttttggca aagaaaaaaa tagtaaaaac
    10621 gaatgcaatc cgtatggtcg aacaaaaaaa gattccctat acagagcacg aatatgagtg
    10681 ggacgaaagc cacctaagtg cgtcatcggt cgctgaacag cttccagaaa gccaatctcg
    10741 tatatttaaa acactggttg ctgtgggcaa tgtgacgggg cctcttgttg ctgtgatacc
    10801 gggagaagcc gaattgaatc taaaaaaatt agcaaaagtt tcagggaata aaaaagtaga
    10861 aatgctccat ttaaaagatt tggaagcaac gaccggatac atacgtggtg gctgctcgcc
    10921 tataggtatg aaaaaactat ttcctactta tttagatcaa attgcagaaa gctatgaaca
    10981 aatcattgtc tcggctggca gaagagggct acagatggag ttagcacctc aagatatctg
    11041 tgcattaacg agcggacaat ttgcagatat aaaacaataa atcatacgta taaaagaaag
    11101 aaggaggccg ggatgttgtt tgtccagtct ccttctttct tttttataca ttatgctctt
    11161 tgcccggcat tcattcctgc tacatgccct gtagcgaaag ccgctgtgat attaaagccg
    11221 ccagtataac cattgacatc cagtaattct cccgcaaaat acagaccatc aatgatttta
    11281 ctctccaatg ttttaggata aatttccttt agagaaaccc ctccgccagt tacaaatgat
    11341 ttctcaatag gaaaagtttt gttgattgat agcttaaact ctttgcatag ttgaacaaaa
    11401 tcttgaattt gtttttgaga ggcttgttgt cctgtcagat gatccatttc aagccgctca
    11461 agaaagaaaa ctaacaatcg ctcaggcaat ataccatgcc aagcattgac caagttcttt
    11521 ttggttgctt tacttttttc tgtcaatata tgaataagct cttgtttggt ttgtgtaggg
    11581 aaacaatcca gagaaagtgt cactggttct cccgttttct ctaattcttt attgacaaaa
    11641 cttgaacaac gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag taaatccatc
    11701 gtatgttcag tcaatacacg acctttttta tttaaaacgc gtaaagtgat gtcttgtaaa
    11761 gaaagccctt gaagtgtttt ctcctgaata tacggttcat ccgatatcaa gggggactct
    11821 gtcgcataaa gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta cccatctcca
    11881 gtagaaccag tagatggata agtccgtcca ccagttgtta agataatggc aggagcatgg
    11941 aactcttcga attccgtgcg tataccgtaa attttatgtt ctttgtggat caatttctct
    12001 actcgagtag agaatagaag cgtcacatct agttctttca gacgattgag caatgcttcg
    12061 ataatcgttt ttgatttatt tgttacagga aacatccgtc catgatcctc ttcttttaaa
    12121 tgtacgcctt gtgattcaaa gaagttcatg atatcgaaat tattccattg cgcaaacgta
    12181 ctatataaaa actttccatt tccaggaata tgtgcaatca aatcatcaac aggacgattg
    12241 ttcgtaacat tgcaccgacc accacctgtc atcagtagtt tttttcctgc ttttttgttt
    12301 ttttcgatca agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc catcatcccg
    12361 cttgttccag cgccgactac gatcacatca tacagttttt ccattccctc acctcaagga
    12421 aagtgtagca tatctgaaga aagaaaacag aaaaaaatgt tttgagccaa aagagaaaat
    12481 gagctaaaat gagggaagat aagcaaagga gcgtggaacg atgacaaacg caacagaata
    12541 tgttcaagca attcaagaaa agctacatca aaaggatcaa gggcaagaag aatttttaca
    12601 agcaatcgat gaattcatgc ccacggttat gtcttttttg gacactcatc ccgaatatat
    12661 cgaaaagaat atcttagaac ttttaacgga accagaaaga gttattcaat ttcgagtgcc
    12721 ttggcaagat gatgaaggaa actggcgagt aaatagaggg taccgtatcc aatacaactc
    12781 agctatcggc ccttataaag gtggattgcg cttccatcct agtgtcaatc taagtatctt
    12841 gaaatttttg gcttttgaac agattttcaa gaatagttta accggtctgc caatcggtgg
    12901 aggaaaaggc ggaagtgact tcgatcctaa aggaaaatct gataatgaga tcatgcgttt
    12961 ttgtcaaagt ttcatgctgg aattgtcaaa acatatcggc ccatcgattg atgttcctgc
    13021 gggagatatc ggtgtaggag ctagagaaat cggctatttg ttcggcgaat ataaacgatt
    13081 gaaaaaatat gatactggtg tgttgacagg taaacctttg gatttttggg gaagtaagat
    13141 acgtacggaa gctactggtt atggattagt gtattatgtc aaacatctat tgaacgaaga
    13201 aaaagattca tttaatcaaa aaacagtttt tgtttccgga agcgggaatg tagccattta
    13261 tgcaatcgaa aaagtgcaag agctaggcgg gaaagttgtt acatgttcag attctagcgg
    13321 gtacatctat gatccagaag gaatagatgt gaaactattg aaagaagtca aagaaatcaa
    13381 tagacaacgt ttgacggaat acgcaaacca acgtccttct gctatttatc atgctggaga
    13441 atctgtttgg acattgaaag aaaaagcgga tatcgctttg ccatgtgcta ctcaaaatga
    13501 aatagacgaa agcttggccg aaatcttagt agaaaatggg atcaaaatcg tagcagaagg
    13561 cgctaatatg ccatgtacag taaaagctgt ttctgttctt catgaagcag gcgtgtggta
    13621 ttgtccagga aaagctgcta atgctggagg agtagcagta tctgctttag aaatgagtca
    13681 gaacgtccag cgattgactt gggaaagaga gcaagtggat gagcagctgg atcaaatcat
    13741 ggaaacgatt taccatacgt gtcgccaaac agcagccgag tacggcgacg aaaaaaatct
    13801 gcttcttgga gcaaatgttg ctggatttga aaaagttgcc aaagcaatgg cagcagaagg
    13861 tttagtttga aaaatgaaac tttcttggaa atattgtcag cattttgttt atttgcttga
    13921 aaaataataa aaaaaaacgt aaagtattac ctgtagagta aacctataag gaggagaaca
    13981 aatggcacac attcattttg actattcaaa agtagcaccg tttgtcaacg aacaagagtt
    14041 agggtacatg caaagccaag taacagcagc ccacaacgaa ttacgggaag gtactggcgc
    14101 aggaagcgac ttccgcggct ggatcgattt accaacgaac tatgacaaag aagaatttgc
    14161 acgtatcaaa gaagcagcta aaaaaatcca atctgattct gaagtattag ttgttatcgg
    14221 tatcggtggt tcttatttag gcgctcgtgc agcgatcgac ttcttgaatc acacattctt
    14281 caatttatta gatagcaaca caagaaaagc accacaaatc ttctttgccg gaaattcaat
    14341 cagctcaaca tatattgctg acttgatcga agtaatcggt gatcgcgact tttcagtaaa
    14401 cgttatttca aaatctggaa caactactga accagcgatt gcgttccgtg tattcaaaga
    14461 gcttcttgtt aagaaatatg gacaagaaga agcaaacaaa cgtatctacg ctacaacaga
    14521 taaagcaaaa ggtgcagtta aagtagaagc agatgcagaa ggttgggaaa ctttcgtgat
    14581 tcctgatgac atcggcggac gtttctctgt attaactgct gttggtttgt taccaatcgc
    14641 tgttagtggt gcagatatcg atgcattgat gcaaggtgcc gctgatgcaa gcaaagcata
    14701 ctcaagcgac aaactagaag aaaacgaagc ttaccaatac gcagcaatgc gcaacattct
    14761 ttaccgtaaa ggcaaagtaa ctgagttgtt gatcaactat gaaccaggaa tgcaatactt
    14821 ctcagaatgg tggaaacaat tgtatggtga atctgaagga aaagaccaaa aaggcatcta
    14881 tccttcaagc gcaaacttct caactgattt gcactcttta ggacaattca tccaagaagg
    14941 acgtcgcaat atttttgaaa cagttgtgaa ggttgaaaaa cctcgtaaat caatcacaat
    15001 tcctgaacaa gcagaagatt tagacggtct aggctacttg caaggaaaag aagtggactt
    15061 cgtaaataca aaagcatttg aaggaacatt gcttgctcat acagacggtg acgtgcctaa
    15121 cttgctagtc aaaattccag aaatggatgc ttatacattg ggttatacaa tgtacttctt
    15181 tgaaattgct gtaggtattt caggttactt gaatggaatc aacccattcg accaaccagg
    15241 tgtagaagct tacaagaaaa acatgttcgc acttctaggc aaaccaggct ttgaagatct
    15301 tgctaaagaa ttgaacgaac gcctgtaaga taagggtttt tcgctagatt taaataatca
    15361 gtgatagaaa catgttcgct ttaaaatgaa aactggtacc catttaggta ctcaaacata
    15421 ctgcatcgtt tattcagaat aaggggctgt gacaagacgt ttgtcacagt cccttgtttg
    15481 tatagaaatc gattgattgc cttgctcttt agtttgtttt aggactttaa gaacagtgag
    15541 gctgacttcg aaggttaatt ctgttttttt gttttctaga atcagtaagc aaaaaagcac
    15601 tcacttcgag tgcttttttg cgatatattc ttagcttaag ataggcctaa tttttctttt
    15661 acagatgagg gtaccttatc gctggtaatt acttttttct ctatgatccg cttttcgctt
    15721 gctttcaact gcaagatcgt tccttcgggg taagcagaac cattggcaga gtcaccagaa
    15781 gtaatgacat cgaaatctaa ctgtcttttt tcaccgtttt cagcataagc atctacgttg
    15841 tatttaaaac ctcttccaat tacttcccca ttatctgctt tgatatcagt ttctgcaggc
    15901 attggagctt tgactaccgc ataataatct ttacctacat aagtagactg atagtagtcc
    15961 caacctttcc atcctataaa agcaatcaat atgataacga tcaaccctaa aatttttttc
    16021 atgaaagatc tcctttttta ttttta
//
LOCUS       DAXLPI010000057        15823 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXLPI010000057 DAXLPI010000000
VERSION     DAXLPI010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.057"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(105..629)
                     /locus_tag="ACF3T5_001603"
     CDS             complement(105..629)
                     /locus_tag="ACF3T5_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFD3632206.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACF3T5_001604"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACF3T5_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFD3632207.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACF3T5_001605"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACF3T5_001605"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFD3632208.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACF3T5_001606"
     CDS             complement(2351..3031)
                     /locus_tag="ACF3T5_001606"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFD3632209.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACF3T5_001607"
     CDS             3157..4026
                     /locus_tag="ACF3T5_001607"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFD3632210.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACF3T5_001608"
     CDS             4052..4567
                     /locus_tag="ACF3T5_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFD3632211.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACF3T5_001609"
     CDS             complement(4614..5177)
                     /locus_tag="ACF3T5_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3632212.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACF3T5_001610"
     CDS             complement(5232..5411)
                     /locus_tag="ACF3T5_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632213.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACF3T5_001611"
     CDS             complement(5488..5997)
                     /locus_tag="ACF3T5_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632214.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACF3T5_001612"
     CDS             complement(6093..6473)
                     /locus_tag="ACF3T5_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD3632215.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACF3T5_001613"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACF3T5_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFD3632216.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACF3T5_001614"
     CDS             complement(7736..9013)
                     /locus_tag="ACF3T5_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632217.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACF3T5_001615"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACF3T5_001615"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFD3632218.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACF3T5_001616"
     CDS             complement(9935..10879)
                     /locus_tag="ACF3T5_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFD3632219.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACF3T5_001617"
     CDS             complement(10881..11567)
                     /locus_tag="ACF3T5_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFD3632220.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="ACF3T5_001618"
     CDS             12041..12361
                     /locus_tag="ACF3T5_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFD3632221.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="ACF3T5_001619"
     CDS             12515..13420
                     /locus_tag="ACF3T5_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632222.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="ACF3T5_001620"
     CDS             13417..14190
                     /locus_tag="ACF3T5_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFD3632223.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="ACF3T5_001621"
     CDS             complement(14371..15795)
                     /locus_tag="ACF3T5_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFD3632224.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
BASE COUNT         4637 a         3294 c         2811 g         5081 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg ctt
//
LOCUS       DAXLPI010000058        15167 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXLPI010000058 DAXLPI010000000
VERSION     DAXLPI010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.058"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACF3T5_001622"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="ACF3T5_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFD3632225.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="ACF3T5_001623"
     CDS             complement(323..892)
                     /locus_tag="ACF3T5_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFD3632226.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="ACF3T5_001624"
     CDS             1078..1980
                     /locus_tag="ACF3T5_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFD3632227.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACF3T5_001625"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="ACF3T5_001625"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFD3632228.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACF3T5_001626"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="ACF3T5_001626"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFD3632229.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACF3T5_001627"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="ACF3T5_001627"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFD3632230.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="ACF3T5_001628"
     CDS             8548..8733
                     /locus_tag="ACF3T5_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFD3632231.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="ACF3T5_001629"
     CDS             complement(8795..9991)
                     /locus_tag="ACF3T5_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFD3632232.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10126..11586)
                     /locus_tag="ACF3T5_001630"
     CDS             complement(10126..11586)
                     /locus_tag="ACF3T5_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFD3632233.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILTKEKGLEQYLF"
     gene            11879..12688
                     /locus_tag="ACF3T5_001631"
     CDS             11879..12688
                     /locus_tag="ACF3T5_001631"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFD3632234.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            complement(12874..15027)
                     /locus_tag="ACF3T5_001632"
     CDS             complement(12874..15027)
                     /locus_tag="ACF3T5_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFD3632235.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT         4440 a         3213 c         2674 g         4840 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaaatgat gaagttgata atgaataaaa tatcaacgat
    10201 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    10261 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    10321 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    10381 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    10441 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    10501 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    10561 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    10621 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    10681 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    10741 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    10801 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    10861 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    10921 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    10981 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    11041 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    11101 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    11161 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    11221 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    11281 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    11341 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    11401 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    11461 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    11521 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    11581 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    11641 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    11701 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    11761 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    11821 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    11881 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    11941 tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc atgtcagtat
    12001 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    12061 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    12121 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    12181 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    12241 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    12301 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    12361 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    12421 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    12481 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    12541 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    12601 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    12661 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    12721 gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc agtttctcgg
    12781 tatgaagtca aatttaagca aaaatgtgag aagctatttt cacaaaattt ttcttcatac
    12841 tcgaaactaa gcggcttttt cacgacctct tttttatttt gccatgaaca tatcataata
    12901 cgcgtccatt atttctttag caatcgtcaa gtttatgtgg tcttgttcgt ctaataagtt
    12961 cggcaagaca acactgatgg caatttccgg attgtctgtt ggtccgtatg ccacgatatt
    13021 actattgact gtcgcaatat ctggatgacc ttccgcaacc gtttctgcag tccctgtttt
    13081 tgcggaaagg tccattttcg cactggctaa tggtttggct gttgtgtaag gatcggtacc
    13141 atggacaact tggtggaatc cttcacggag gatcgccata ttttctgcag aaatattgac
    13201 cgtattttct actgttgtgc caatttcttt cttcacttca cccaagtttc cattttcgtc
    13261 attgccatag atcgctttga ctaggtgcgg ctgtactcgt ttcccgccat tagcgactgt
    13321 tgaagcatat tgagctaatt ggattggtgt atatgtgtca aactgtccaa atgccaaatc
    13381 ggtaaagtta cctggagcat accattcttt gccattatcc gcattgaact tcttcatata
    13441 atcaacagaa cgtgagatcc caggagattc attcggcaaa tcgatcccag tcgttgtacc
    13501 aagaccaaac tctttgaaag agtctctcaa ttgctgataa gctttagctt cttcatctaa
    13561 actaggtagt cccattccag gtgtatagtc tagtcctaac atctttaacg ccactttgat
    13621 catatacgta ttagaagaca attccgatgc tttgactgca tcaagagcaa cttgtccgct
    13681 tcgattaaag acagagcttt tttctgaagc accttgcaga cgaatcggct catcaatcaa
    13741 aacttgattt ccgctgatcg cgttacttgc ccagcctgct gtcaaagtac cagccttgac
    13801 tacagaccct ggatcaaagg cgctagtaat cgtgcctaaa gcattctcgg tgatttcttt
    13861 ggaaccttgt tcatgactga aaccagtcat tgctagcact tctcctgtct gaggattcat
    13921 agccactgca taagcaccag gcgaatactg tgctttgccg ttgttgatca atgtttgata
    13981 attccttttc aaaatttctt ctacttttga ttggaattct gcattcatag ataacattaa
    14041 attagaacct ttttccccag agaagatttc tttttgattc gaaacatttc cttcattatc
    14101 aagggaaact tcatattggg ttttggttcc ttgcaggaca tcttcatatt gtttttccaa
    14161 ataactttgt cctacacggt cattccgaga ataacctttt gctaggtatt tctctgcttc
    14221 atctgctgga agtccttgct tttcagtcgt cacactgcca agaatacttt tcagtgaact
    14281 tgctgcatta tattctcgtg tccagtctgt gccagtcgaa actcctggaa gttcagaagc
    14341 gcgttctgcc acaacagcta actcttgatc agtaacacca gagtttttga tatagacagt
    14401 gtttaattca tatgcactat tcatccgttt aaagatggta gcaattttta attgctcctc
    14461 gtttaaatta atctcatcag ctgtgacttt attgaccatc gcactatatt gttcgctgtt
    14521 ccctaattgc ttttcttttg ccgaaagtct ttttgtggct tctttcaaat gtttgggatc
    14581 agccagccaa aaatcttttt tatctctatc tgtcagattt tcatctaccg gaacatcgat
    14641 cagcgagttc agcttcgttg cgatttcaag caaatcttta gccgtaactt gtgttcctct
    14701 cgtatatgtg atggcctgat tcgctttatt cgtaactaaa gcttttcctg tggagtcgta
    14761 gatcattccg cgaggagtac tttcttggac cgtaatcgta gaagtagact tcactcgcgc
    14821 ctccatctct tcgccgttca cgatctgcaa ataacccaat ctagcaatca acgcaacaaa
    14881 taacgtaaat ataatgaaaa aaagaaaatt taagcgaaaa ggaacatggg accggcgaaa
    14941 cgttttgttc ttaggttctt gtgcaccttt tcttattttt tctaaccaag aagtatttaa
    15001 tatttttttc ataaaatctt tcatcatttc ctatccttcc gcctatttac attcatgctt
    15061 cattgtaccc aaaaacttct taataaaaaa gaaaaaatgg gcggagcaag aaattcttta
    15121 cctaatttca agataaaatc aactttttgt ttcacagcaa tcactta
//
LOCUS       DAXLPI010000059        14814 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXLPI010000059 DAXLPI010000000
VERSION     DAXLPI010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.059"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(744..1085)
                     /locus_tag="ACF3T5_001633"
     CDS             complement(744..1085)
                     /locus_tag="ACF3T5_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFD3632236.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVIRPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1085..1315)
                     /locus_tag="ACF3T5_001634"
     CDS             complement(1085..1315)
                     /locus_tag="ACF3T5_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFD3632237.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            1611..1949
                     /locus_tag="ACF3T5_001635"
     CDS             1611..1949
                     /locus_tag="ACF3T5_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632238.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPIQIET
                     LVQRISTREDFAIVECEIVAIFVEEGILHDPTHINVDKWQPLIYKLREYTTANQSLSL
                     NFRFQEFKES"
     gene            complement(2154..2729)
                     /locus_tag="ACF3T5_001636"
     CDS             complement(2154..2729)
                     /locus_tag="ACF3T5_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HFD3632239.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPEQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDVSFARKIITQQMPELVMEKV"
     gene            complement(3094..3675)
                     /locus_tag="ACF3T5_001637"
     CDS             complement(3094..3675)
                     /locus_tag="ACF3T5_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3632240.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASETGKGQFYYYFS
                     SKKEVCLAVIHAHMVVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWIKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3858..4484)
                     /locus_tag="ACF3T5_001638"
     CDS             complement(3858..4484)
                     /locus_tag="ACF3T5_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFD3632241.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFSLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4506..5834)
                     /locus_tag="ACF3T5_001639"
     CDS             complement(4506..5834)
                     /locus_tag="ACF3T5_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFD3632242.1"
                     /translation="MEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTCI
                     NIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARFL
                     SNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPEK
                     LVIIGAGYIGLEFASMFNEYGSKVVVLDAHPEFLPREDEDIAQMILEDMTNAGIEFHL
                     GVSVDQVADQESTAAVTFTENGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDRG
                     AIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVPY
                     SVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETDL
                     ILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5993..6394)
                     /locus_tag="ACF3T5_001640"
     CDS             complement(5993..6394)
                     /locus_tag="ACF3T5_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFD3632243.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6480..6686)
                     /locus_tag="ACF3T5_001641"
     CDS             complement(6480..6686)
                     /locus_tag="ACF3T5_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632244.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6781..8283)
                     /locus_tag="ACF3T5_001642"
     CDS             complement(6781..8283)
                     /locus_tag="ACF3T5_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFD3632245.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(8301..8756)
                     /gene="argR"
                     /locus_tag="ACF3T5_001643"
     CDS             complement(8301..8756)
                     /gene="argR"
                     /locus_tag="ACF3T5_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFD3632246.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8854..9471)
                     /locus_tag="ACF3T5_001644"
     CDS             complement(8854..9471)
                     /locus_tag="ACF3T5_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFD3632247.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9692..11065
                     /locus_tag="ACF3T5_001645"
     CDS             9692..11065
                     /locus_tag="ACF3T5_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3632248.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            11140..12252
                     /locus_tag="ACF3T5_001646"
     CDS             11140..12252
                     /locus_tag="ACF3T5_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD3632249.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(12324..13961)
                     /locus_tag="ACF3T5_001647"
     CDS             complement(12324..13961)
                     /locus_tag="ACF3T5_001647"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFD3632250.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(14129..14737)
                     /locus_tag="ACF3T5_001648"
     CDS             complement(14129..14737)
                     /locus_tag="ACF3T5_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFD3632251.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         4049 a         3045 c         2609 g         5111 t
ORIGIN      
        1 actgtattga tagtgtacct aaaagctcta ctgacataag atcgtgtaat cggtaaagta
       61 attttattaa ttatacggga tgtaatcaat tagagaataa actaatcata aaaggtactt
      121 ttacattcat aattgtcgct ttgagattct actttggttc actatctgac ccaattgcac
      181 attttcgatc attacaagta ttggtgtcgt tagaacaact aaataaaaac tagcggtaga
      241 tttttcagaa tctatgctaa tttctgtatg gatgttccag tgatggcaaa tttctctttt
      301 cattgttttt cttgtcgcat gatatgcctt tttgtgttat tcagttaaac tatagaaatt
      361 ctttcctaga cagatcttga tcccaaattt tacgtttttg agcatcgata tagtttaaaa
      421 tgacattact catatctaaa gagtttagat tttttaggac taaaactgtc agttgttctt
      481 tttcttcttt ctgttaaagt attgtcgcga cttttcgtat acgttaagat atgtcgtgaa
      541 aaaacaagtt ttgtgacaca aaaaaacgaa tgacttgagg aaatcacctc aatcattcgt
      601 ttaaaatgct atatattctt tagtttaaga tacggaaatt aatgccatcg accacactag
      661 tagaatgaac gacatttact tggccttttt ttaatttcgt tatgtttagc atataaaaat
      721 attatagagt ttacttacaa tgcttaaaac atatactgta taatctgatc aattttagcc
      781 atgtcttgca atggtaagtt ttctattttt ttgagttttc gttccttaaa atcaagagat
      841 tttaattgtg taataattac ttgtcctttt atatctagct cttcaggtaa tgagtaacgt
      901 gtcggtaaat tctttatagt ggaagtaatt ggacgtatga ccgaaaacat tgttttacgg
      961 ttaaattcat aacgagagac gactagtcct ggtcgtcttt tttgaatttc ttttcctacg
     1021 gagggatcaa aatcaatcca tacaatatca ccttttttag gaatataaat tctttcttct
     1081 gacactataa aatttctctc ccttcaggag ttaaatcttc ccattcgtct ttctcataat
     1141 actcggcctc ttccccacca ctaaatggat cttcaatttt tggaactaac gtaattgttc
     1201 catcatcaga atacacaacg atatattctt ggttttctga aggtttcttt ccgttatcag
     1261 aaggtaaagt taccactaca gaacttcctt gtaatctaga ttttgtaaca agcattatca
     1321 tcactccctt aataaaagta tactatataa cactaagtaa cgcaaaattc ccctttgtaa
     1381 actatctaga aaattgtttt gtttaaagta tatcttattg gatataaata gaaataaatt
     1441 gtagccagtt cattttttct ttaaataaaa aaataatcca agaaacattg aacctatgga
     1501 tccaaaagct acccaaatac tatagctaac tccccgctac cttcaatctt gcagataaaa
     1561 cgctttggaa aaaaatcgag aaaatcgcaa agacgacagg ccatcctatc atgcctgatt
     1621 ataaacaaaa agctggctat tccttttgct ctgacaaatt tcaattggga aagttcacga
     1681 aactagcagg tgaagaagtt tcaactgttc ggattaaaga atgcccgatt caaatagaaa
     1741 cgttggttca acgaatttca actagagaag actttgctat tgtagaatgt gagatcgtag
     1801 ctatttttgt agaagaaggg attttacatg atccaactca tatcaatgtg gataaatggc
     1861 aacctcttat ctataaactc agagaatata caactgccaa ccagtcgtta agtttgaatt
     1921 ttagattcca agaattcaaa gagtcataat gataaacatc attaggtata aataaaaaag
     1981 actcgcagaa cgagtaaaaa acaaatagaa gctatacctt agaaaggtcg actgtgactc
     2041 tatagatgca gtatatgcag cttatgagca tttcttctgt tctctttgtt tttgggatct
     2101 gtgctgaagt ctttttcgtt ttttgccgct ctttctttga aaaatcgatt atgttatacc
     2161 ttctccatta ctaattcagg catctgttga gtaatgattt ttcgcgcaaa agaaacatct
     2221 gttacccaag gttcgatttg ttcttcagtc aagacaagcg gcattcggtc atggattggt
     2281 gcgactgtgc ggttaggttc agtagtcatt atgattgatt ctgccatttt ttcatcctga
     2341 tggctacgat aaaaaccgcc aatatacact attttttcct tatttgaaaa cataaatttg
     2401 tctttttctt cattccactc ataaaagcca ctcattggga aaacacaacg attttgcaaa
     2461 aatggttgat gaaaggtctt tttttcttct actgtctctg cacgggcatt aatcatcaat
     2521 tgtccttttt taaatccggt aaatccccac ttcatgattt ccggagtgag gatgttttct
     2581 tcctcttttc ttaacgtcac aacaagattt gacggaaaaa cttctccagc tgctaattct
     2641 gctgcctgct ccggttgttt cgtctgggct tctcgataat aattctgaag gagctctgac
     2701 tctagatcaa aaaaataacg accacacatc ctaatccctc ccaatctttt tttctatcat
     2761 acactgcaac aagaagtccg taccaacaaa acgctttcaa tttgtgtaga actaaaaaag
     2821 ttgtgacaag tgacaaactc tccagtgaat ggtattctcc tgccaactcc tcggtcacaa
     2881 gtcacaatat tcaacaaaca tgagaagctg ctttttgaaa ttgttccttg tgactcggag
     2941 tgaaacggca ggcttacgac ctctaggtaa acggtgttcc agaagtagct tcttcgaaaa
     3001 ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt
     3061 gaagctgacc acttctgtca caacctctaa ttattaaaga accttttgcg aagttaacaa
     3121 tctttctttg atcaattgaa aattttgtct aagtagtgat agatcctgtg tgactttcaa
     3181 caataagata cttccctgga tttcagcaat caacgctttg gcttgacggt ctgcttcttc
     3241 atcggacagt ttttcttcag aaagtgccag gatattttgt ttcgtatact tgatccattg
     3301 attgactaat tctagtagca acagacggta ttcttcatcc actgtactca actcaataat
     3361 caaattacct acaggacagc catgataagt gatctgtgtc tcatgatcag caaaaatcca
     3421 gtccaacatt tcttctaatg cttcttctgg ctgttttcca gcatttaaga tgtcttcgaa
     3481 caattctgtc tgccacacga ccatatgcgc atggatcacc gctagacaaa cttctttttt
     3541 tgaagaaaag taataataaa attgcccttt ccccgtctca gaagctgcta gaatatctcg
     3601 gattgacgtt tcatgatagc cttttttata aataagatct ttggtcgctt ccaagatctt
     3661 ttcctttgtt ttcaatgata ttctcctttt tcatttctca tagtcctata ttatacaata
     3721 aattcatcat tttttcttct taatcgttta aaaaagactg tgccaaatta tctgacacag
     3781 ccctcactat aaaactgttc tcatatagct agatgttcat tgattcaaga actgcgagga
     3841 caaaagcaat tcctatctta tgatagttgc tctaatactt cctctgttgt catcacttga
     3901 tctgcaataa agccaaagtt cgtcaacgct gcatccattg cttcttgacc aggagcacca
     3961 attgcatctg tcgctaccac aacattgaat ccactctcta ccaattcacg catatgggaa
     4021 tcgctacaca agtttccgtt cattccacca agaatcactg tgtcgattcc attttttctc
     4081 aattggagcg ccaaatcatt gctttctggt ccaaagattt tatgaggaga gctgataatc
     4141 gtattttcat ccaaatatgg tttcaattct tcgatgaaat cagctcctga accttcttca
     4201 actgattcat attgactttt cctagcaaac atctgttgct caagcatcat atgttctcct
     4261 gcaccgccaa attgccattt atggtcatgt gggtaaaaat aatgaggaga aataaagagt
     4321 ttgtatcctt tttcttttgc ttgtttcacc atttcgatca tgttttcgat cgtattgtat
     4381 ttttctaaga tatcttttgt caaactaaag ccaccgcctg ctggtttcaa gaactcgact
     4441 tgcgggtcag tgagaactac tgctgtattt ttattattga acatggtgga ttctccattc
     4501 gttatttatt ttaatacatc atttaaagct tcactcattg ttggatgaga atagatttga
     4561 tctctcaata acgtataagg aagttttcca ttcatagcta gcgaaatcaa gttgatgact
     4621 tcatgagaat cttccgcata gatacttgcc cctagaatca agtcagtttc agggtcgact
     4681 aaaactttga atacaccttt aggatcttct aatacttgtg ctttagggac accgcttgtc
     4741 atatacttga ataatttgta attgacgcct tgtgctttgg cttgtttttc tgttaaacct
     4801 acattggata aagtcggagt gataaataca ctgtaaggca cagctttccg gttattcgtt
     4861 gttcgtttat tctcgccctt caactgatcc ataatgattc gataatcatc tagagagata
     4921 taagtgaact gcaatccgcc tttgacgtct ccaatcgccc aaatgttgtc tgcatctgta
     4981 cgaaggtatt catccacagc gattgctccg cgatctgtta gtttaacatc tgtattttct
     5041 aatccaagtg atgctgtact tggttttctt ccagttgcag ccaaaacttt tgacgcttgg
     5101 attgttactt cttggccatt ttctgtaaag gtgacggcag ctgttgattc ttggtctgct
     5161 acttgatcta cagatacacc tagatggaat tcgattcccg catttgtcat gtcttctaaa
     5221 atcatttgcg cgatatcttc atcttcacga ggaagaaatt caggatgggc atcaagaacg
     5281 acaactttag atccgtattc attgaacatt gatgcaaatt ccaagccaat atatccagcc
     5341 ccgatgatca ctaatttttc cggtaattgt tcctgatcca tcgcttgcgt agaatcaatc
     5401 aagtagcgac ttgtattcaa tcctttgatt ggtaaaatga ttggttcaga accagtattg
     5461 atgaaaatcc gttcgccttc aacaatcact ttttcaccat tattcgtcac ttcgatttga
     5521 tggttagaaa gaaaacgtgc tgtaccgtcc atcactgtcc cagttgcctc atcgctgatc
     5581 atatgataat ttttggcgcg aagcatgccc gtcaattttt ctttccgagc aacagcctct
     5641 gtaaagtcga ttcctctttc tccattgaaa atcaatgatt tagaaggaat acagccgatg
     5701 ttgatacatg ttccgccata catacgtgtt gacttttcaa cgactaaaac cgattcgcct
     5761 tttgaagcta acgccttcgc taaagtctta ccgccctttc caaaaccgat cacaatattt
     5821 tgatattttt ccatcctttt tccctcgctt ctttttttac ctaccgattg gtacaaaaat
     5881 atgataaccg ataattgtct attctccaaa tcatctgctt tatttgtcaa aaaaaaccag
     5941 acaaaaagcg gtcctttcac gtttttagtc tggttcgatt tcctttattc ttctaaacaa
     6001 tcgttacttc tacatcgata tttcctctag ttgctttgga gtaagggcag aattcatgcg
     6061 catcttctgc aattttttgg gcttcttctt cgctgatgcc atcgatatgc acttctaata
     6121 ctacaccgat ctgatagccg ccatcatcta aattataaag agagacacgt gcttttactg
     6181 tggactctgc tgtcattcct gcttctttga tcatatgttg taccgcccca ttgaaacaag
     6241 aagcataacc tgctgcaaat aattgttctg gatttgttcc ttctgcatgg attcctggag
     6301 gagtgatttt catatgcatc gagccatttg gtgcctcgac ttctccattg cgcccgccat
     6361 ggttgatcat tgctgtttcg tatgcttttt tcattgtact gctcctttcg attgaccatt
     6421 tgctacttgg ctataattat agtgtatgac aagtaaggcg ctttctacaa aaattctgct
     6481 tattcgaata ccagaatgcc gtcttctact atttttccaa aaaacaaaaa aatactactc
     6541 gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa tcttttctac ataatctttt
     6601 atatgattga ttttaagata catatatacc agaaagttga ccccataaac caatactgtt
     6661 aaaaaaataa ccatattcgt taccattttt tcctcctttt gctggtctcc ctaccagcac
     6721 tcgatttaac cataccactt tttttgccgt ccaagcaact agcctttcaa aaagactggt
     6781 ttattctgct gttaatgatt caagatcaga aaaatgtccg aataattttg ctaattttac
     6841 taacgccaac ttgttctttc cgaattcatc attttctatc tgtaatacta gtaacggctc
     6901 atgtaagctt aaacggatca aaaaccagcc gtttccgtat ggctcttcta gatcatatcg
     6961 aataccttct tcattttccg ggtcgaagtt catccctttt tcttcttcca acagtcgctt
     7021 catttcttct aatatcttgt ttccttgttc gcgtggttca tcgctgaaca attttaatcg
     7081 gatttcttgt acttctacgg gctgtttcaa ctctttgatt tgttcatcta atgtgatacc
     7141 ttttcttttt aattctggca aaagcatcag gatctttgcg atgacatatg caccatcatc
     7201 cagaaaatag ttttccttga atgcagcgtg tccgcttgtt tcgatggcta gctgtgtatc
     7261 tttcccaaga cggttaagtt ctaatgcttt attgattaca tttcgatagc cggaaatata
     7321 acgaacttgg atgcctcctt tttcttcaat gaatctttgt agatgagaag aagtagggga
     7381 attcgtcacg attcttgttc ctggatgctc ttttaatacg attgtactta atactgcgat
     7441 caaattattt cgattgatca ctgttccaga agcactgaca actgcagaac gatccacatc
     7501 tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa acagcctgct tgatactttc
     7561 catcgcttct ttattatcag gatttgggat atgattagga aatcggccat ctggttccaa
     7621 aaattgggaa cctgttgaat ccgcacctaa gctattcaat actttttcag caaagaatcc
     7681 accagcacca tttccggcat caacaatgat tttccaacca tttagtggtt tgctttctag
     7741 tgttccaatc ccctttttaa tttttgtgac taaatcttgc gcataaggtg tcaaaaggtc
     7801 tgctttttct acgcttccag tttgttcgct ttttattttt tgctgctgtg ataggataaa
     7861 agcaatatct tctttttctg ctcctccttc tgaggtaaag aattttattc cattataata
     7921 atagggaaga tggctagcag tcagcataac cgcagcatcg cacgagaact gttcaaattg
     7981 agtggacata aacatagcag gcgtagtagc taattcgaaa tcgatgacat gtatcccttg
     8041 ttctttcaga ctttggatca acgcctcttt cagtgctggt ccgcttatcc tactgtcatg
     8101 accaatccca atcgtccatt ttttatttgg atcggttgat ttcttctttt tctttatcca
     8161 gtttgctagt ccccagccga tcttttttac catgtctgtc gtcaagttta cttcatatcc
     8221 ttcaactggt aatgcgattc ctcgaatatc cgatccattt tgcagcgctt tcatatcttt
     8281 catataaaag tcctcctcat ttattccctc attttttcca gcttttctgc aaatcgctgt
     8341 gcttcttctt ctgagcggca aataataatg aatgtatcta caccggccat cgttccgaca
     8401 acttcagggt aagccacttc atctagccaa ttcgttacca catcggcacc atttcgttca
     8461 gtcaatacga taaccatgaa ctgaacagac tggatacgca aaacctcgcg tctaattgct
     8521 gtacgcaaac gttcttcatt taaagaaaca gatggctggt taaataatgt atagcgtact
     8581 attttgtttt cattttgact ttttactaac ttcatttctc gaatgtctcg agaaatcgtt
     8641 gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa gcagctcttc ctgtgtccca
     8701 ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc gctgtttctt tttcatatta
     8761 gacacctctc aatgttgatt atagcaaaag ataagtaata tttcttcata ttttggtatt
     8821 ttttactttt attttttgtt ttcattcagg ttgctaacct ttttctattt taacacgcaa
     8881 aatcgtcttc attttttcag gtgctgattt tcttggatcg ctgatgtaaa tctctttgtg
     8941 ttctttagaa gctctttcat agccttcttt tgcgatgaat gcttctaatt tctcaaacgt
     9001 tgctggttct tcggcatagg gcccgacgtg taagatttgg gcacaaagac cttcttccat
     9061 ctttttgaat actacttgtt ctttcaagtc ttccggaagt ttgctagaac gctgaatggc
     9121 ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg atcatcaatt gataagaaaa
     9181 atgttctttt ttcatcactg gttcatgcaa atacttttct tgcagtcccc attgtccttc
     9241 cagtggatag acagtataca tctgataatc aggaatcagc caattttttt taggcgacat
     9301 gcgtattgca tagctgactg catatagaca ggctactctt cgtttaaagt cttcctgatt
     9361 cgggtcgccg atccctctga tgctaaagaa tgatttttta ggaacctcta gtatttgcgg
     9421 gctttttttc atactatagc tcttgtcttt acgccattca attttttcca tttttcttcc
     9481 tccgcgaaat tccatttagt tatttattat catacctctt tttgagtgac ttggctaatc
     9541 gtgttgacac tatttttcag tttgtgctag aataagtgaa gtaaataaat cgtacagata
     9601 acacgtttcg gcgtgtcgag agagaggcgg tttctatttt tataatagaa atcactctct
     9661 ttttttacga aattttttag aggtgatgta aatgacaaaa caaaaacagc tgcttttagt
     9721 acctgcagta attgcaacag ggatgatgtc ttttgcgggc gtattgatcg agacggccat
     9781 gaatgtaact ttcccaactc ttatcagaca gtttgattta acgactgcac aagtccaatg
     9841 ggtaacaacg atttatcttt tgatgatttc gatcattgtt ccgctgtcta cttatttgaa
     9901 tcgcaatttt agtctcaaaa aactcttttt agcttcaaat ctgcttttct tggcaggtat
     9961 aatgatcgat ttcttctctc ctaccttcat ggtcctattg tttggtcgat tgctccaagg
    10021 tgcagcgaca ggtatcgctc ttccgttgat gttccatatt atcttgacat ttgcccccct
    10081 caatcgacga ggcgcgatga tgggtgtcgg tactttgaca acggccattg ctccagcaat
    10141 tggccccact tatggaggca ttatgacttc acattttaca tggaatcata tcttcctgtt
    10201 tcttgtgccc gttttgattc tttctttgat tatcggctgg tttgctattc ccgagatggc
    10261 agtacaaaaa agtggttatt tggattggat aagcgtttta ggaatttgtc ttttgtttag
    10321 tggaagtcta acatttttga gtacgttggg aacagtttcc agctggattg ctttaatcat
    10381 cggcggtatt ggctggttcc ttttttacta tcgaagtaaa aaagtggagc atccactgat
    10441 ccgactgtct attctttcaa ataaaacatt ccgcttgttt ttgattggct tcttagttta
    10501 tcagttttta ttacttggtg tatcttttgt cttgccgaat ttccttcaga tcgttcaagg
    10561 aagtagtcct tttgtcgctg gattagcgat gcttcccggc gcagcggttg gtgctttgct
    10621 ttctcctttt tccgggaaaa tgctggatca atatggtccg aagaagccaa tttttgctgg
    10681 gctgattctt tctttgattg gttggctagc attattccta ctcataggac acgcttcagt
    10741 gggtgtattg attgcttgtc atgtgtttta tatgatcggt atcggattat cttacagcaa
    10801 tatgatgacc accggaatga atgcactgaa tgaagaacta caaggagacg ggaacgcaat
    10861 gttcaatacc ttgcagcagt tttctggcgc tgtggctact tcattagtag cggtcattat
    10921 caattacgtc cagcatcata cttcccataa ctatgaagtg tctactacat taggatcaaa
    10981 agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc tttgctcgat tcgcctatta
    11041 tctgttcttt gctaaaaaag cgtagattcc atatacggca ggtgttgcat ctgccgtttt
    11101 tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta tggaaaaaat tgccattatc
    11161 ggtggcggga tcattggtat gaccctagcc agttacttag atacatcaaa attcaatgtt
    11221 gctttatttg atgaagggac aggacaggca actaaagcta gcgctgggat tatctcccct
    11281 tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg ctaaagacgg cgctgctttt
    11341 ttccctaaac ttataaaaga tttccaactt gatcaaacga tttacgcaca atcagggaca
    11401 cttttgcttc gacctgaaaa agatttagct gaattggctg ttcttgccga gaaacgaaaa
    11461 gaagaagcgc cagaaattgg aaacatcgaa tggctttcac ctgaacagac atctcaagca
    11521 cttccgttat tgaaaccagc agtttcactg cgtatcagtg gtggtggtcg tctggatgga
    11581 aaagcatatt tagaacatct tggtcagcgt ttagcacaaa aacaaatgga gcagatccag
    11641 gcaaaagtca ctttgcataa gaagagcgat tcttggctca tcaagggacc gtttggagaa
    11701 tacacagctg atcgtgtaat tttgacacct ggcccggcac ttaagccttt attacaagaa
    11761 ttaggctatg ctgcagatat acgtccgcaa aaagggcagc tcttagcatt tcagacatct
    11821 tataagatga gtcaagactg gcctgttgct atgcttgacg gagaagctga tttcattcct
    11881 ttcatggatg gaaagatcct tttaggtgct acccatgaaa acgatcaagg ctgggatttg
    11941 gctcctacta gcgaagctta taagggattg actaagagta cagaacagtt cttgactgat
    12001 ccagatcaga taaaaaactg gccatacgag ttccgtgtag gtacccgtgc ttatacgtca
    12061 gatttttccc catttttcgg cgtactgcca gatgatccaa gcttactggc tgctagcggt
    12121 ctaggctctt ctggcttgac cacaggtcct tatatcggct atctattagc caacttctta
    12181 aatactggtc attggcctac ggaagattat caaaaggaca tctccaccta tattacgaaa
    12241 aatccatcat agaaaaacag ctttttcaag taaggatccc gagcgatatt gcgcatggat
    12301 tacttcttga aaaagctgtt attttagttc aatttctgaa tcaaattctc caaactttct
    12361 tttgcttcat ctggcaagag atcttttcct atctgagctt caatgaatcg taaacgaata
    12421 tcaagtcgtt gtttcaccag atttttatag gacgtatcct caaaagaaac aggaaaatct
    12481 ggtataggta aatagtggat gtcttcaaat ggaccaaacg acttgaattc tgccgtttgt
    12541 tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt tgattttttt atcatcgaaa
    12601 tatccagtat tcaggacata gcaggctgtt tgctgtttca aaaacaactc tcgaaaagct
    12661 tgatagtctt ccctaagagg atagacacgg aaaggattag caaatggttc aatgactagc
    12721 tcatctagct gtctttttgt agtgatattt tccgcgtctg atcgttttgt tgctaatgtt
    12781 gcaccaaaag acgctgctaa aactgggtca ttgatcttga taattggcgg aagcgaatca
    12841 tctttcatga tccaaaaaac agcatcgatc ttttcgctaa gatgatcgac tcggtcgggt
    12901 gtcgcataac gtgatttgac tgtccgccca tttccgtttc gtatatctcc tgttactagg
    12961 actttttctc ctttacttgt acatgtcacg cccacattct gacaagtaag gaaagaagaa
    13021 atggccggat catcagacgg atagtcttgt gttttatcaa agtaagaagg ttccaaggca
    13081 gtagcaaaac cattttttcg agaaataacg aaagcatcgt catgcaagat cgaaacagag
    13141 tgaccttccg tatgttttgc taaggtaatc gttgattttc cagaaccgga caacccaaat
    13201 acagccatcg tataggtctg gtcattacta gcatattgtt tcatcccacc gtggcaggca
    13261 ataaagccat tgcgatttcc caatgcccaa gcaagcgtca aagtcgcttt ttttaattct
    13321 ccaaagtaac gtaatcctag cacagccgct gtattatgga gagcatcaaa caaaatgagt
    13381 ccatttggaa atgtttcatg tgtccattcg ggatcacaaa agatataaag atctccctca
    13441 tcaaaggaaa cagacttttg ataagtctct aagatcttcg aattcgctgg ctgaaaattc
    13501 agcaaataag aatacaaatt gttctcaaat ccttctggaa tcagcaaatg actttgcaca
    13561 gaaaattctt cagataggcc aacgattact tttgtatggt aaaactggcg cttgcgactt
    13621 tgatacacag cttctcttaa aattttctcg tatttgcttt gttccttgtc taagtcctca
    13681 aagatccgcc gagcacttgc tgttcttccc acaatgcttc catcattgaa tactagtgtc
    13741 ctaacatcag ctggtaaatc tagttcttca gcatgtgaga ttgttaaatc tgtgatgatc
    13801 gttcctggac tcccttttgc tagttgataa gctgtcttcc tatcaaaaac ttcatgaaca
    13861 ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac tggataacag tggatttcca
    13921 cttgtgattt cgtttaattc gagactctct tttgtactca tggctcctcc tcattcccta
    13981 aacaaaaaaa cgttttcttt attatacatt attcgagttt ttatttgtac tgctgcgctg
    14041 tgggaagcaa acgagttatt tttcacacaa aaaaaccgag aacttgaata aacaagtttc
    14101 tcggtttcat cattttcctt ttatcggctt agtaccagcc gtttgctaac cagaatgttt
    14161 tagcagcatc ccatgaacca taacgagatg ttacatattg ttctgctaca cgttcttgat
    14221 tggcagctga atagtcgccg tttaagtatg aagcatctaa ttgataacgg ccgatgtaac
    14281 gtccgttagt agctgtgtat gaaccgcctg attctttttg tgcgatccat tcttttgcag
    14341 agcttgaagt tggcgctgtt gcagcagctg gggcagcagg agctacttca actgtttcaa
    14401 caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt tgtagatgct gtattttctg
    14461 ttgttttgtt ttctgttgtt gctgaagaat catttgaagt tgggattgtt aattgttcac
    14521 caacataaat gcggttgatg tttgcgatcg aatttttttc agcaattgca tcgatcattg
    14581 agttgtctcc tgcaaatttt tgtgagatct tagataatga atcacctgat tttactgtat
    14641 aaacttcatc agcgtgagca tttgtagttc ctacaaacat tgcagtagca ccagcagcaa
    14701 aagttgtacc taaaacaatt ttcttaaatg aattcatatt aaactccttt ttttctccgt
    14761 tttttattct ttcgacgcta ttaactttat catttagaaa tatttcatag gtaa
//
LOCUS       DAXLPI010000060        14718 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXLPI010000060 DAXLPI010000000
VERSION     DAXLPI010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.060"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            83..844
                     /locus_tag="ACF3T5_001649"
     CDS             83..844
                     /locus_tag="ACF3T5_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFD3632252.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            1072..1266
                     /locus_tag="ACF3T5_001650"
     CDS             1072..1266
                     /locus_tag="ACF3T5_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632253.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            1535..1834
                     /locus_tag="ACF3T5_001651"
     CDS             1535..1834
                     /locus_tag="ACF3T5_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632254.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1931..2365)
                     /locus_tag="ACF3T5_001652"
     CDS             complement(1931..2365)
                     /locus_tag="ACF3T5_001652"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3632255.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(2597..3307)
                     /locus_tag="ACF3T5_001653"
     CDS             complement(2597..3307)
                     /locus_tag="ACF3T5_001653"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFD3632256.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(3297..4157)
                     /locus_tag="ACF3T5_001654"
     CDS             complement(3297..4157)
                     /locus_tag="ACF3T5_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFD3632257.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(4162..4800)
                     /locus_tag="ACF3T5_001655"
     CDS             complement(4162..4800)
                     /locus_tag="ACF3T5_001655"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFD3632258.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(4815..5114)
                     /locus_tag="ACF3T5_001656"
     CDS             complement(4815..5114)
                     /locus_tag="ACF3T5_001656"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3632259.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            complement(5157..6641)
                     /locus_tag="ACF3T5_001657"
     CDS             complement(5157..6641)
                     /locus_tag="ACF3T5_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFD3632260.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            complement(6661..7122)
                     /locus_tag="ACF3T5_001658"
     CDS             complement(6661..7122)
                     /locus_tag="ACF3T5_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632261.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(7140..8207)
                     /gene="ulaG"
                     /locus_tag="ACF3T5_001659"
     CDS             complement(7140..8207)
                     /gene="ulaG"
                     /locus_tag="ACF3T5_001659"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFD3632262.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            8425..9195
                     /locus_tag="ACF3T5_001660"
     CDS             8425..9195
                     /locus_tag="ACF3T5_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFD3632263.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(9468..10295)
                     /locus_tag="ACF3T5_001661"
     CDS             complement(9468..10295)
                     /locus_tag="ACF3T5_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632264.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            10862..12097
                     /locus_tag="ACF3T5_001662"
     CDS             10862..12097
                     /locus_tag="ACF3T5_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3632265.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(13096..13356)
                     /locus_tag="ACF3T5_001663"
     CDS             complement(13096..13356)
                     /locus_tag="ACF3T5_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFD3632266.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(13383..14684)
                     /locus_tag="ACF3T5_001664"
     CDS             complement(13383..14684)
                     /locus_tag="ACF3T5_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFD3632267.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4682 a         2977 c         2361 g         4698 t
ORIGIN      
        1 tataatcaac aaagaaaatg tgattttttt cactatattt tatcacatga aaattcatta
       61 agaaagtagg aatcgaagta ttatgcacac accagaagaa attatggaac tcagtattca
      121 tcttggaaca aaaaaaatac ataaacctat cttagctaaa ctgattttag gttttatcgg
      181 aggagccatg atttctctag gttatttggc atacgtacga gtctctgctt ctatcccaga
      241 atctatggct agcttgacta gtttagttgg tgcctcggtt tttcctatcg gcctgctggt
      301 catccttctt ggcggtggtg aattgatcac cggaaatatg atggctgtgg cgattgcctt
      361 tatggataaa aaagtcacat tctcacagct ggttaagaat tggtcagtga ttacttttgc
      421 aaatattatt ggtgcaattt tcgttgccta tttctttgga cactttgttg gtttaactca
      481 ttcaggtgtc tatctcgacc aagtgattca tattgctgat cataaaatcg ctgtttcttg
      541 gtggcaagca ttaggctccg gaataggttg taattggttt gtcggtttag cgttatggtt
      601 atcatatgga gcaaaagatg cggccggcaa agtactaacg atttggtttc cagtcatgat
      661 ttttgtggca atcggtttcc agcatagtgt ggccaactgt tttgtgatac ctgctgccat
      721 atttgaaggt caagcgactt ggttagaatt tctctcgaat tttatccccg tttatattgg
      781 aaatatcatc ggcggagcta tttttgtttc aggtttttat tacgttagtt ataagcatca
      841 ttaagattga aggtaatgtt cattcgagca ttaccttcaa tctctttata tactatttat
      901 attttaagaa caatagcctt ttattatata tattattcaa attagttata tcgagtgaaa
      961 aaaactttca aattgattct ttttatttat cataaagata tagatacata atcattttca
     1021 cttacatata tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat
     1081 gtagaagtaa aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa
     1141 tatcctttac ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa
     1201 ataaagaacg agctagaaaa agaactgact tgtggcaaag ctgttgtaga tatcatcgaa
     1261 aagtaaatcc aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa
     1321 gattatagaa ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt
     1381 ataccatatg attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa
     1441 gatgaatcaa atatacttgt cattcatcat gaaatataga gacttataaa attgtttatt
     1501 tttataatgt atcagtatag aaaggaaata aaacatggat tatactgttg aatttgacag
     1561 tgaactactt aagcaatttc cacaagaaga acatttcatg tataaagaag caatttccca
     1621 caaagtgaca gagtatttgg agcataattt ttttcgtata aagccagtgc gtactcgttc
     1681 ggcaaaagga atatacgaga tgaaagtcaa aattaataaa aaaaattatc gcatagcatt
     1741 tgctttaaaa aaaaatcatg tgttcatttt ttatattagt tcgaatttgc aaaaaattgt
     1801 atttgataaa gaggtgagta aaaaaataaa atagctgagc attggaggat agattgagtg
     1861 atactaagtc tatcctcttg tttttctaaa gaactaagct agaatttttt cttttgtacg
     1921 ttattatatg ttatttgtca ttaccaaaaa gcaccttttt tcttctagta tctatgacat
     1981 aagaaatacc acccatttcc tttacttgaa caaatcctac tgattcataa aatttttttg
     2041 ttttctcagt atcatctgtc aataattcga tttggactgg tccataacta gtgatcttct
     2101 ccaaagcttc attcaaaagc actttgccta ttccttggcg ctgatacctc ggatgaatga
     2161 ggatatcttg aataaatgcg atcgtcgcaa aatcagtgat ccaacgaacg attccgataa
     2221 tcttcccttc cccattacgc tttatcagag attcagattg ttcaaatgct ctagctaacc
     2281 ttgcagtgtc ttttgtgtaa ttcgtccaac caacgctctt atataattca gaaatctcat
     2341 cgatcataag ggaatcaata ttcatttttt tcttcctttc ttatacaatt ctccctctaa
     2401 taatacagtt ctttgactat ttaatctatc acatataact ctcctgtaaa gattctctcg
     2461 attccttaaa atctctcttt ccttttagaa tgcatccttt ttgataaata aaaaggttgt
     2521 gataaatttt cttcacaacc tctgtttctc tctatcaagt gttatctttt aattttctac
     2581 ccttacccta gcatctctat tgcccgtagt atgcatactt tccatgtttg cgaaaataat
     2641 gtttatctag taaatattgc gggatttcac atatattggg gttgatcagc tcgttttctt
     2701 tggccatcag acaaatttca tctaagatca aactgttttc tactgctttc ataggtgaat
     2761 ccccccatgt gaacggtcca tgtccataga caagtactcc agggacttcg tttggatcta
     2821 attttctttc cttaaacgtc tcgacaatca cattccctgt atgaacttcg taagcttctc
     2881 ttacttcttc tttcgttaat tggcgtgtac aaggaacctt accataaaaa gcatcagcat
     2941 gagtcgtgcc ataagctggc aaatcgcgtc ctgcttgcgc ccacatgacg gagtgcgtgg
     3001 aatgagtatg agtaatagca gttatatctt caaaagtttg atacaaaaca acatgtgtgg
     3061 ggagatctga agatggtttt aagctatctt cttctattat atttcccgac aaatctgtta
     3121 ccaccatttg atctgcttgc atgcattcat atctgacacc gcttggctta atgacgatga
     3181 ctcctagctc acggtccact tcactcacat tgccccaagt caatttgact aatccgtatc
     3241 gcggcaatgc cagatttgct tcaaggacac gttgcttcat tgctgcaatt ttttcttcac
     3301 aagacatcat agccagcctc cttcaattta gaacttaagt agtcttttgc ctgctggatt
     3361 tctcttcgaa agtcttggct tttttcactc cacatttcaa tcagaaatgt tccatcataa
     3421 tctagacgtt tcaaatgtct taataatcca agaaaatcaa cacaaccgct accaaaagga
     3481 acatcacgaa actggcctga atattctttt gttacagggt aagtatcttt taagtggata
     3541 gcagtgatat aatcgatccc tttctcaagt tctcgagccg gattattttc cggccatgcg
     3601 gacaaattgc ctaggtctgg atatacttgt agaaaagggg aatgaatctg ttctttgatt
     3661 tctaaaaatt ttgaaatgga gttcataaat ggatcatcca tgatttccac ggaaagaatg
     3721 atgccatatt tagaagcttc ctttacgctt tcttttattc cttcgataaa gtcttctctg
     3781 gaattcattg atttctcttc ataataaaca tcgtatccag ctatttggat tactttgacg
     3841 cttagattat gcgccaaatg gattgctttt ttcattagtt gtttcgctgc ttttttcttt
     3901 tctggatcag cagaaccaaa cggaaatcgc cgatgcgcac ttaaacaaat cgaatggata
     3961 cgtacaccgg agctgaacat ctcttctctc agctcagcta tcttttcttc agaccaatct
     4021 aatcgtgcta accgctcatc cgtctcatca atcgacatct cgataaaatc gaagtccatt
     4081 tcttttacaa gggaaaaacg ttctttccat gaaatatctt tgggtaatgc tttttcataa
     4141 atcccaattt gagccatttc cttaccccca gatacgaatg atctcatctt tgaagttttg
     4201 tgcggccttc atcggatcat ttgctgctgt aataccacga ccagtgataa aggtatacac
     4261 atcgatccct tcaaataagt gtaatgtgtg tggatttaat ccaccagtta cagaaacatt
     4321 aaagcctagt tcaatcagtt ttttgatctt cgataaatct ttttcacccc aagtttcacc
     4381 agataacagc gcatcacggc tttgatggta gattacctgc cggataccga tgttatgcca
     4441 atccatggct tgttcaaaag tccaattgcc gtagagttct acttgcaatt cacctatttc
     4501 tttttgtgct gcctccatag ttggaatcgt tgcacaacaa atgactgtca tccagtctgc
     4561 tcctgctttt gcaacattac tcgccactgt tcctccagcg tctgcacatt tcgtatcagc
     4621 gactagtttt ttatctggga acatactgcg aatacagcga atcgcttttt gtccttcctg
     4681 taagcaaaga attgtgccca cttcaataat atctactaca tcaccaactt tcataacatc
     4741 tcctaatgca tgttctaagt cattgtgatc taacgcaatt tgcaaattcg gcctgctcat
     4801 cttagttcct tcctttactt gtttttttgg taccactttg tttcagcaaa tttttcactg
     4861 atttctttgg ctgacatcac atttttgata ccgataactg taacttcttt tttctctgcc
     4921 tgagtaaaca tattcaaaaa attgactggc gtaaaaacta catcgtattg gccagctgaa
     4981 ctttttccct ctgaaatggc acaatgatgg attttggtaa ttggtacgtt tagttctttc
     5041 attgcttttt ccacacttct catcatcatc aagcttgttc ctgatccatt tgcacacgat
     5101 actaaaattc tcatcttaat ccctccatta tgagtgttgt ctaatcagat tttcttttat
     5161 actctctgct tttctgcata tgtgtcatag tcttctacaa tcaaaaaata accctcagga
     5221 tttttccgat attgtaactg cggaatagcc aataaaccaa tgacaactac ggcaattcca
     5281 gcataaccta ggactttcat gattactgta aacaatggcc aagctgttgc ccagtcgaac
     5341 atcccgatat aaccaccata tgttgacatc ccaatccatg tagcgaataa tgctgaaccg
     5401 cctacttgta tgattcctga aagaaacggg aacaggcaag cggctttgaa tccacctcgg
     5461 ttattggcat aaactgctat aacggcatta tcaaagaaca acggaatgaa accggctatg
     5521 acgatcgttg gtgatttcaa taaaatcagt aaaccaatca tcaaaaactg tcccaaagca
     5581 ccaaacaaga acccaatcgt taccgcattt ggcgaaccaa agccaaaagt agcagctacg
     5641 tcaattcctg gaacagctcc tggtaaaatt gtgtttgaga ttccttggaa agattgagtt
     5701 agttcatcga cgaatgtccg aacacctaat tgcaagatcg ccagatatac cgcaaaattt
     5761 aaagaagtcg tcataatgta gaataagaaa ctctgtcctt cttgcatgaa ttgcgcttga
     5821 atcaaataat ctttgcctag tacaatcaga atgatcccaa agaaaaatag catgagtaac
     5881 gaagtagcaa ccatattctc attgaaaatc gacaagaatc ctggtaattt gatatcttcg
     5941 atttttttgt cttgcttgat tgatctattt ttcttttcat cccgcttttt catccactca
     6001 gctaattttg caaatataaa gattccgaac atttgctgat gtgccactgc aaatccggca
     6061 ccttctgtca attcctgcgt gattcctact gttaagttag aagctactgc ccaataacaa
     6121 cctaaaatca gtcccataaa aatcaatact tgtatcctac ctagatcagg aaagcaaaac
     6181 agtaaaatcc aaaaagcggt agctgcttgt tgaacctgaa cgtttccggt agtgaaaacg
     6241 gaacgcagtt tcgtatattt ctgaaaacgg actaataaga tatttaaaac aaatgcaatc
     6301 agtaataaaa tcatcacatc tccaaaggta cggccaaatg tctccattaa tccagcatcc
     6361 acagcatttt gtccaaaata aggatctgta accatggcat ccaaattgaa ccgttccttt
     6421 agtccaacca aaatcggccg aaaattattg accaaaccac cagagccaac agtcaaaata
     6481 aagtaaccga ccgttgcctt taagaatcca gcgatgcatt catgaagcgg gcgtttcagc
     6541 aaaatatagc ctaataacac gataaaacca atcaggtatg ctggctgcgt caagatattt
     6601 gttgcaaaat acgtccaaat gcttaacaaa aattccccca tcttttttct cctctatcaa
     6661 ttaattatat tctgccatca caacttggta atcatcgatg gagttcgtag agatgagacg
     6721 ctcaaccaat ccatctgtca ttaataattc ggataatttt tggatatttt ctagatgttc
     6781 ttctggatct tttgctgcaa gtgtaaaaaa caaactagcc gttttctcct catcctcccc
     6841 ttcactttca aaacggattg gctgtttcaa cttggtaaat gaaatagctg ttccaaaaac
     6901 accatcactt ttttctgaag aatgcggcat tgctacttga ggtgcgatca caatataagg
     6961 accgtatttt tctacacaat ggatgatctc ctccacatac gtttcatcaa taatttcctt
     7021 atcgatcaat acaagacagc tttcggcgat agcttcacgc caatccgtga tttctttatc
     7081 tgggtaatga atcaattgat ggtcaaaaaa gtatttcagc aagaaactcc ccttctttct
     7141 tataaaaacg acggaaacgg tgtatcattt tcaatcgaga agacatcttc aaatcctcga
     7201 taaaaattaa actcaagatc atctttgttt gtcggataga tgaatttccc gcctacttgc
     7261 cagatataag gtttaaatcc atacttcagg cgatcctttt tgaatttcca aatttcagtg
     7321 atctcttttg aatcagccat aaaattagac caaatatcgt agtggacagg aatcactact
     7381 tttgcattca atgactccgc catgcgcagc atatcaacag aagtaacttt gtctgttatc
     7441 cctcgtgggt tttcaccata ggcccctaaa cagacatcga ttttgtgttc attaccatgt
     7501 ttggcaaata aattggagta atgtgaatct cctgcatgat aaagattccc tcctgatgtt
     7561 tcgaataaat agtttactgc aatttgatcc atctcctgag gcatttttcc ttttaatacc
     7621 tcttctggat cttcacaagt gaccaacgct gtccgatcaa acgcttctaa agctactaca
     7681 ttgatgtctt tgattttgac tgcatcacct ggctttactg taatcgtttt ttcttcagga
     7741 attccccatt tttgccacgt gcgaaccact tcttttggcc caatgaatgt tgcttcagga
     7801 caattttgat gaactgctgc ggctgtatgg atatctagat gatcggaatg gatatgtgta
     7861 acgattaaag cgtctacatt ttttatcgca aaaggatcga ttacgaaagg ctgagtacgt
     7921 aaattaggct gcatattttg aacgccactc atacgcatca tctgatggcc ttttttcatc
     7981 atgccattcc catgactacg ttttcccgtc ccacaccata gatcacataa aatattcgtt
     8041 ccttcatgac ttttcagcca aattcccgta cagcccagcc accacatagc aaaagttcca
     8101 ggatccacta cttctgtttc gatttcttca ttcaagtatg tgccccactc aggaaatgtt
     8161 tctactaacc acttttcttt tgttacttca ttgacagttt tactcatttg cttgctcccc
     8221 ccatatcgtt gttccaactt acaagtaaat attaatgtgt atcagcatcc atggaaaccc
     8281 ttttatatga ccgtttgttt tttatatgat tttctttcag taatcaatga ttgtttcagt
     8341 ataataaaga cagatatgaa tgtttaaatg aacaactagt caaataaatc catggatcaa
     8401 gatgtccctc aggaggcaaa agtcatgaaa aactcacttg tcaatattga aaaacgtcaa
     8461 aaagaaattt tggaactgct tcagcagact gaacaactat caactttcga attagctgaa
     8521 tcccttgatg tatctattag tacgatacga agagatttaa atttattaga agaaaaaaac
     8581 gagatcatac gcaattacgg ttattgttct tataattaca aaaaccagac tgattttgat
     8641 caatctggtc ctgaaaggat taaacaagcg attgccagag aagctagtaa atacattaac
     8701 gattatgata cgctgtttat caattctagc tcgactgcgt tgaaaacact ggattttctt
     8761 caatctaacc atttgacagt cattacaaac aacgtgaaaa tcaattactc tccgcacaaa
     8821 gataattgta gttatatttt aactggtgga gagatgcgct ttccaaaaga agcactagtg
     8881 ggcgacatcg cattaaatac gatttcttca gtcaatgcag acatttgtat cattggctgt
     8941 agcggagtcg atgtagtaca tggggtcaca acaaagatat taaatgaatc aacgatcaac
     9001 gaaatgatgg tcaacaagac aatcaaatgt aagattttag tcgctgatca tcgaaaaatc
     9061 ggactgacat caaagttcaa gattgctgat atttcagtct ttgattattt gatcactgat
     9121 aaattttgtt ctgaaaaagt attagaggag atcagaaata cggggataaa aaccattcaa
     9181 gtagattcat tttaagaacc aggacgtaaa tatgtaaata ggattatcaa aaaaatgaat
     9241 aacaaattta tcgataaatg aaaaacacgc tacgctcaaa ttgataaagg aatcccaaga
     9301 tgtgtccatc caatcgatac catcataatc tattgcttct tttataaccg ttatatgttg
     9361 cctaaatagg aatcaaacaa tatcgaaaaa aggaggtatg acaaaagtca cacctccttt
     9421 tttcgaataa acggtattca aatcagaaca ctgatttcac aaccacttta tttcaggtcg
     9481 ttttcaatca cctttaccgc gtaatcattt ccgttgaaac gcgctatttt ttttgcgtcc
     9541 atatatcttt ctaaataatc atcttctcct aattgcttat ggcaagaagc aatttcataa
     9601 agcaagtatt ccaatgcata agtcgattta tgtttcttat tatactcaat acccttctca
     9661 cataattgga ttgctttatc ataatcctcg attttacggt aataggtggc gatatcataa
     9721 tatacaatgc agaccatcag attatcaaaa tcagtctcac tcaatatttt aagtgtacgc
     9781 tccacataaa ctttcgcaaa atctaattct ccttttgaca agtagatcat tgccacgttt
     9841 gcagctgctt gtaagtgatg gatatctgtt tcggtcgttt gttccaacac ttgattaaaa
     9901 taaaataatg ctgcatctgg ttttttcgca taaagatgat caagcagtcc ttgataaaat
     9961 aaaatttgat ttaaaagatc agaattactt actttttcct gatcgatcgt ttgtaaaatt
    10021 ttataagatt cttggtattt tgatacagaa ttcaatgatt gaatctcatt tatttttttc
    10081 ctgattttgt cttcttctgt ttctactaat atatcggaga tctcgacatt cagctttttg
    10141 caaagcgcac ttaagatatt gatgcctggc gacatattct tgttttcgat attgctgaca
    10201 gttgcttgtt cacaaatgcc ttctgctaat tgtacttgtg ttaacccctt tttctttcgg
    10261 tattctttta gtaatgctcc atctacttta atcaaaacaa tttccttccc tttaaaaaat
    10321 tatttctcct ttatatcata cacaaaaaaa agttctaaac aaccaaaaat gtttagaaca
    10381 gaataaaaat tttaatttta aaataatatt caaattaaac aactggcgta tctgctcctt
    10441 gatttctttt tgtgttatct gaataattat cgtttacagt tataacacat agtgaaaatt
    10501 gtcatggcga gaaagtctaa gaatattttt aaacataact atttctttct tacgtgtgta
    10561 taatgacgac agcacaccta tcaattaata taattatttt attttttatg ttctcatgtt
    10621 tatttttaat gacacaactt ctcgtattta tctggcacaa catataaatc gatcaaaaaa
    10681 ttgttgcgtg tttttttgaa aattggagtt tcaacttgat tacaaaattt aaatttagaa
    10741 gttgtgaaaa cagcgagaga cagtttaata ttcaaaatat acataggcta ctcgaacgct
    10801 gtgtttttta gtatactagt aagaaacgaa gggatagact cattccaata aggaggattt
    10861 catgaagaca attctttttg ctcctgcagt attcaattta gctgagacaa cacgaatgat
    10921 tgaggttgcc aaagctatga aagaccgtta ttactgtgta ttctttggct tttctaaaac
    10981 ttttgctcct ttgatcacag aaagtggctt tgagtatcat ttactagcgc cgatccttac
    11041 gaaaaaacaa gaagaacaaa tcataaaatt tgaccagttg cgtacactta aaaacccttt
    11101 taccaaagaa atggttgcct ctcgtatcac tagtgaacag agtctgattg ctaagtttga
    11161 gcctcgactg attgtgacag gttcgaatgt gacaattttt ttatcggccc gcaataaaaa
    11221 gattccttta gtatatgtta aaccctatgc tttgagtgaa cctttttttc aatcagagca
    11281 tgcgctgatg ccccacgagt tacgtttctt cggcccttta agcaatcccc tttggcactg
    11341 gacaaaaaag cgtatagtaa atacccgatg gatccccaat gccttcaaac acgctgcaca
    11401 aaaagaaatg cttcctttac caaaaacaag tttacaaatg atggatgctg atctaaatct
    11461 catcacaacg cctaaactat ttttggaaga agaacagctg ccggaaaatt atcgaatcgt
    11521 tggtccaatt tttgctaagc tcagtacttc cttgcctgaa gaagtgctaa cttttataca
    11581 gcgaaagcaa caagaaagaa aacgtattat ctatttcgca atgggtagtt caggcaatcc
    11641 gcgtatgtta cgcaaaattt tgttcttctt acaaaaacaa ccggatctag cggttattgc
    11701 acctattgac tatctgcttg atgacattca atctgactca gaagatcttt taataacaga
    11761 atatctgtct agtcatcttt tgcatgaatt cattgatttt tcttttatcc acggcgggga
    11821 agggaccgtc caaactgcct gtgaatccgg caaaccgttt atcggtgtgg ggatgcaatt
    11881 tgagcaaagc tgtaatattg actattgtgt aaaatacgga aacgcaatcg ccctcacaaa
    11941 accagtgact gaaagaaaac tgcagattgc tctgcgcgaa gtttcttcac ccaagattcg
    12001 tcagcaggca cgcacgctgc aagccgtttt tccaataaat ggtccacaac aagcaattca
    12061 agaaatcgaa accttcctga gcaaccaccc aatatagttt caagtagaaa aagctttttt
    12121 aatagagact catcagtaat ttcgctattc gagacccaga cagtgaaaat acaaaaaaag
    12181 taaatagtgg attgaaaagt tagagttact taaattacgg tgtctaaaaa acacaaaatc
    12241 atccttaaaa taaaagatct gacgcaatcc aaatgagcat aggtagcgtc tgccatttat
    12301 tcttagtcaa attctttgac acctttctct ggttggatca tctcaacgtt tcttcaaaag
    12361 aaagaccata ataattaggc aaatacattt tcttaaaatg tgtcatatag atctagaagc
    12421 atcgctttgt agagatttgt gctgctagtc ccttcaacaa ttactaaatc aaatgtagga
    12481 tttaccactt tatctttgat aatcaacctc cctcttctgt tgagacaatg atctcgcaat
    12541 ccgatgcata ctcctatata aactgccagc aatccccaca tgctgagtag atcatgtcag
    12601 aatgttctat gtcgtcagca acgataaact aaaagtcacg ttcaccatga ctgattgcta
    12661 ttcccagcgc aattatttct atacacgttt catgatttga ataaacactc tcatcataca
    12721 tattaccatt catacaccat actgcagccc ctactatatg gttgtaagct cccaaatcta
    12781 tatttgcctt gatcactttg gtagtagatc gactaattct taatccattg ctgataaaat
    12841 tttcattttt gcctccttct ttctgccaaa acagttaaaa caataataat ctttatgtaa
    12901 aaaagggctg tgacaaaagc cttgtcacag tcccatattc cgaataaacg gtggtcaaaa
    12961 gcttgcatct gcggtaataa gttcaaccaa acgaaaaata agccaacatt tcacaaaaat
    13021 ttaagaagca attttcgtaa atttcttctt atttcttgaa gctaaccact tctgtcacaa
    13081 ccacttttag aacatttaaa gcttcgatgt gacaaaccat ctatgctgct cgatcataaa
    13141 attgatagca aaaattccac aaagtacata tgtcccccat gaaaacaaaa cattcatatg
    13201 cggtacaatg atctttggaa gaatcacgat caaaaatgat gccagtgccc aaatccatcg
    13261 attgatttga atatgatatg tagctagcat attcaatata aggtgactag ccgttaataa
    13321 aaagataaaa acaaaaaata aaatcagcca atacattgtt tcccaccttc taaaaaccaa
    13381 acttagacag tttggacttc gttttctcca cgactttgtt catctgacag catcttttta
    13441 tcataagctt ttacaaatgg ataatagata ataaatgcta tcaatgtgtt gatcatgatc
    13501 aagataacaa cacgccagtc atttcctgct gaaagataac cgccgattgg agctggtaat
    13561 gtccaagttg gcaaaacagt aaatctattg accatttcta atttgactgc aaaataactg
    13621 ataatagtca aaactaaagg aactaagttg aacgggatga acatgtaagg attcaacatc
    13681 ataggagctc caaaaatcat gggctcattg atattgaaga taccaggaat aatagaaaaa
    13741 cgaccaacct ctttcaagaa ttttgattta cagaagaata taaacaaaac agataccacg
    13801 atcgttgctc ctgcaccacc aattgttacc gtccattgat aaaactgttc aggagcgata
    13861 tagggtaatt gggtggcagc tgtaccatct gccaatgcct ttgcattctc atccaacatg
    13921 atcagccaca ttggacgaac aacagcccct acaatactca tgccatggat accaaatgac
    13981 cacaacaaat ggatcaaaaa cattggtagt aaaacaccaa gcaaattatt ccctaaaata
    14041 ttggttaaag gctcgaataa cgataataag gtagcgttta catcaaagcc cgcgacgctt
    14101 cgaatcagcc aagtaattgt taaaatgact gcacctggaa tcaatgcctc aaaactacgg
    14161 gcaacagcag gtggcacttg ttctggcatt ttgatagtaa tatttcgatt tttaaagaaa
    14221 cgatatactt ccactgcaaa aatcatagat aagatccccg caaacatccc agatgctccc
    14281 aaagaactca taggtaagac ccagccaaga ggtgcctttt caggagttaa aacgtttaca
    14341 ggaacttgta acataaagaa cgttcctaaa gataatatac cacctgtcac actatctaat
    14401 ccatatgatt tagctagatc cgaacccata acaaaagttg cataaatagc catcaatccc
    14461 atcgtcatcc taaaaggtaa aacaatcgtt gccgaataag gggcaataaa ttcattccat
    14521 ccaggaaacg gtaaattgat aaagaccata aaaaatgtcc caatcaaaat caatgacaat
    14581 gttgaaacaa ccccattacg aattgctaat aaatgacgct gtgtaccaat ttttgccatt
    14641 ggtgggacaa gtttctcttc gacccatttc atcagctttt ccactaaaac cactcctttt
    14701 caataaaata tgaaccat
//
LOCUS       DAXLPI010000061        14686 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXLPI010000061 DAXLPI010000000
VERSION     DAXLPI010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.061"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..966
                     /locus_tag="ACF3T5_001665"
     CDS             <1..966
                     /locus_tag="ACF3T5_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobA"
                     /protein_id="HFD3632268.1"
                     /translation="MLSKISSERDMLTKANEILDKQAERFFKKSYPSLNIDKFSNHEV
                     RAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKKIFKEKPFQTSRYLDSKIKQVED
                     SITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDSVIEGEMLLAK
                     ADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKH
                     IDELSPLAKQNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVK
                     MFQFAKSINRLLSGNQLQKKRNLDKLIKQTKAKENQSLQRNIPLR"
     gene            990..1322
                     /locus_tag="ACF3T5_001666"
     CDS             990..1322
                     /locus_tag="ACF3T5_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CagC family type IV secretion system protein"
                     /protein_id="HFD3632269.1"
                     /translation="MKKFIKDTKFKLGSAVVALGTLFITDPVFAATDPQAKLVQAGNT
                     IKGVLTALIVVVGGIACAKIVIKYLPSIDDPQEKNTMYKALGTALLVTALGGALVWLV
                     PWAYGLLA"
     gene            1341..1724
                     /locus_tag="ACF3T5_001667"
     CDS             1341..1724
                     /locus_tag="ACF3T5_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3632270.1"
                     /translation="MNSDQVKQALLDLLNADTEKGRTWFFPSNVSDRYTVILGLDLKQ
                     SAKAIGTALISVLLAILIFRSTAVFPLIIYVIVGLVSFGGVWAFYTIKPITDRPNISI
                     SDFMKQRKDFSKRPKVYYKKPKERV"
     gene            1741..2370
                     /locus_tag="ACF3T5_001668"
     CDS             1741..2370
                     /locus_tag="ACF3T5_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632271.1"
                     /translation="MFDFLKKSSKSNDNKKSDTIKEMIYWEDSSRFQGVFKDFFVVYQ
                     PEKKQFSLVSMLKVDGLNVDTLPVSEQEGLNEDFGVFLSQNVLYEPQITSKNVPVEID
                     DFVEAWGNTVENYRKMPGHNEALLQLKASYYYHYRNLASNMETSKKQHFVINSEPISK
                     ETYDSLELSYQVLRDKTRTIRTALIAFLSKYDCQVEICTIGEMKKVLNS"
     gene            2381..4342
                     /locus_tag="ACF3T5_001669"
     CDS             2381..4342
                     /locus_tag="ACF3T5_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002425003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFD3632272.1"
                     /translation="MEKIPKEKIIVIPEVDTDVVSDLAPFNFTVERDKLLIDDSYAVP
                     YVITKYNNKPRGNWFNRIRKMSGDITISHYYTKANGNSLNDYYNRTIKNKQAEIDRSH
                     DPLTIIRLEREMKIAQTQLEQAVDENTSYLYLYTYVLIKSKSEDKLKKLCEDFETRCI
                     ASGVKALIPYTMIDKAYWSALPLQSNEVPEYTYTIANSISASSIFPFDDNELSVFTKN
                     MIIEGINKDTENIVSIDYTNRKLVVNRNKFVFGLSGGGKTTYLTSDYLKKYAFADNST
                     ELKHRIVLFDPEDEQTERVRSLGGEIINLSSMSDVRINPFQIYSRNTPDVDLKESLSD
                     FEEDELVENLEIKHKDFEMTENDIDKEISKRMNILTPYFLMVDHSLTDSQLSIIKLEA
                     KKCYTTLYEKKNLSKMENTDFPTFSDLESRLKALEETDPKRYKRIEDFIYSLEDFTIG
                     SRTIFNGHTNIDLSNPLICFSLRDLQTEEGIRDLAYLNSFSYLFEEITNNPQIVTSVY
                     ADEFHFLLKNKISADFFFQAYKRFRKYNADCTVSTQQIDDVLKAPDNIGKAIIGNSFT
                     KVFFGLDETEAQGISNELKLKLTKKELSFITSKRQGEALLFHGTKRAKIKVDLTQEEM
                     RLLNPGGYEDIYGVSPKKEINWLLRSKIQ"
     gene            4356..5708
                     /locus_tag="ACF3T5_001670"
     CDS             4356..5708
                     /locus_tag="ACF3T5_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFD3632273.1"
                     /translation="MKYKILKNLQFYYQENVIVVQINEKYLTNREHIFDVEESEQYFV
                     DVEEILTKDGKLEIVYHRPNGYTPLLDLKEYADFYKLDIVNRLLEMNVLEKTNTYLAM
                     QNILLKDTRDLLFIYKADHFGNLPYSTKEELEQWKNFICSFFGKFSLEKYEKNRIEIL
                     TKEKNSFLNDVEAVESLESLRDLIKNRLTEEQKNFFSAELQDKKANVRKIRRNKSLKI
                     ALVVGVIALYGGTVFLMKVNEKKQVTATQQSAETEITILNKIIDNDSENIEEDMQKLN
                     YPKKKQVDIYVKLGDYTKAYELDKKSDKKIIQSLYKQGETEKIENLDLPGSDYLADFK
                     KILAYDNSTDIEYLVQTSTDTTIVEALIDQAVKKKDIPTVKNIRQVSITQKKLAIDPK
                     RQISMIDLLIENNSEELENIYKDNSLNDDLKKKQTNDLLEENNTLLSEKIELTNAEKD
                     "
     gene            5730..6839
                     /locus_tag="ACF3T5_001671"
     CDS             5730..6839
                     /locus_tag="ACF3T5_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HFD3632274.1"
                     /translation="MGATAAKLLIRYRKPIAKAILGFFVFIIVIFLLIFNNDQTAPSG
                     GLATENKNLSESVLRYKSTVEKYARQYDGMEYVPYILALMQIESGGNGGDPMQSSESL
                     GLPPNTIQDPERSIQRGVEFFVENMKSAINRGSDIKTALQAYNYGGGFVDFVVKNGGT
                     YSFELAKQFSINMSGGEKVPYVNALSTSMGYNYRYNYGNMFYVPKLLEYVNESQASAE
                     GVQNNSNNTGAVQVDVPSEYKNKLRYPRYDGHNYNTSGSYPFGQCTWYAFNRMAQIGK
                     PVDDFMGNGGEWGYKGKALGYKVTNKPKVGTAISFPPGSFGSDPVYGHVAFVEVVNAD
                     GTLLVSECNVVNPGSGTISYRVVPASIANIASYVE"
     gene            6852..7403
                     /locus_tag="ACF3T5_001672"
     CDS             6852..7403
                     /locus_tag="ACF3T5_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632275.1"
                     /translation="MEFDKIKVSLFISTLALLVVTIGMIVFGIKQGEQIRHLKSDLKS
                     VQTELADKQAEQNTNQSESEKIIKEFYKTVYNYEKSQKEISMTTVKELATDNVYQELQ
                     NEINVNNSYSPQQNTIQKSSVNENEIKILAYASKDNSQQYLVTAPIHQVFNGTKNDFE
                     INQIIQIKNQQITQRTTIQLGEE"
     gene            7408..7839
                     /locus_tag="ACF3T5_001673"
     CDS             7408..7839
                     /locus_tag="ACF3T5_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632276.1"
                     /translation="MAKKKQEQVKLIILVILYLLLLPVTLIVFKLLPFIIANDTNIEL
                     STFVTTILKHPINTFLEIIQSNYMIPFFIVQIIVLIILFVFLHPTKRQPFEVVGKTNP
                     VHGSAYWGEESEINAPKNVHLIPEKSMKKILEKSMRRGKNE"
     gene            7832..9487
                     /locus_tag="ACF3T5_001674"
     CDS             7832..9487
                     /locus_tag="ACF3T5_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFD3632277.1"
                     /translation="MSSLLAESDGLILGKFQSGNVVIQPEDSKISNRNIFVVGGPGSF
                     KTQSYVLPNVVNNRSTSIVVTDPKGEIYELTNEIKKAQGFKTVVINFKDFLLSSRYNP
                     LLYIRKSNDTNKIANVIVSAKNDPKRKDFWFNAQLNLLNTLIKYVYFEYEPSARTIEG
                     ILDFLEEFDPRYNEEGVSELDEQFERLPDGHEAKRSYYLGFRQAQSEARPNIVISLLT
                     TLQDFVDKEVAEFTSTNDFFFEELGTSKICLYVLISPLDRTWDGLVNLFFQQMFTELY
                     FLGDKHNAKLPVPLVMLLDEFVNLGYFPTYENFLATCRGYRISVSTILQSLPQGFELY
                     GDKKFKAIIGNHAIKICLGGVEETTAEYFSRQVNDTTIKVYTGGTSESKTSAKTTNRS
                     GSKSESYGYQKRRLITEGEVINLQQEENGRKSIVLIDGKPYMLRKTPQFELFGNLLKK
                     HEISQQDYISSQTEFAKEYIEELEVQHKQRKVQLASAPVFHEKKQENDLNKKEIKLPE
                     QPIESEVTEEVEEKEQLMSDILSSFDEGIEENTTNNDNSELPF"
     gene            9505..10428
                     /locus_tag="ACF3T5_001675"
     CDS             9505..10428
                     /locus_tag="ACF3T5_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632278.1"
                     /translation="MNGLKEFFGNIPMFQKQVEREIEEKGIKPSEGIKKMEFSRQVLR
                     NIVIGVLFIGILAFVGISYFNNKNQPPVAPTATTTSKESNQSEASGEATENSSQAVQG
                     SSDHLLKLSAKERADEATEAFESWYKSFSKGDVILEINKELLKEGSGGTSPIELQTKL
                     IDNLKAKFGDKVSDDFYSGLQASFNFNPVIVDGTKGLTISKQNDDQSQWLRTWFLDTE
                     KTAQNTKIILRNDFSYEWADWRNKGQHDEKVGSMFTQIDWANDLRYEVIGIVTSKQEK
                     NIENIGGVFVVMQYNEKIGKWQVSGTIGGVM"
     gene            10430..11362
                     /locus_tag="ACF3T5_001676"
     CDS             10430..11362
                     /locus_tag="ACF3T5_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbL family protein"
                     /protein_id="HFD3632279.1"
                     /translation="MDVIDQIKETGSDILSSAGDAISGIFEGVKESVDSVLHPIDSLL
                     GSWGSDLLEASMKIANKTTFDIPNIKLISTITNVFVFATTTFAICIVLYKVIEAQIQA
                     SNGSGDALTANIVQKLFYSSIALATLPWAMNFFIKKIVAPIGEYVIQQVANDLNVETI
                     GERLRNFLVSNFFNGGVSAVVLFVFVIFFAFSIAKYFVAICVFYADFLVLTMLTPLVA
                     LSLLTDEQNYFQIWIKELLSEALTMLIKLILYLLMITLMIAEKYTLPNFMLMVGCGLL
                     IIKTPSALQNMWYSTKVSKGGGLGNLAMMAMFRK"
     gene            complement(11489..12676)
                     /locus_tag="ACF3T5_001677"
     CDS             complement(11489..12676)
                     /locus_tag="ACF3T5_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFD3632280.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
     gene            12999..13967
                     /locus_tag="ACF3T5_001678"
     CDS             12999..13967
                     /locus_tag="ACF3T5_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632281.1"
                     /translation="MSYYFEIRIILPEEEKQFLNRKLSKSELSEVTHYLQQKTSRGIP
                     VKFRVGIFRVEDQTKIMSVTLNTKNTKETDVINLLLNRVTDQHVLVYLNEPTEPTLNT
                     QELNRQELKTSNERQEIPQTEIPTETVNEPSVIKEISKKNQAKQTNSRKESLSESITK
                     KNVPKIHLFISILTLFIVLLIGISVIQQVQLQSVKKESELLKEQIERVKETDISQSKI
                     DTFGRYFLTYYFSQEKNQENYQSSLRTYVSEKVDIADWKALEKMLKSVNYYGSEQTKK
                     GYSVEYLLNVSVDNRSKMQKITFEVEPTKNGFLVTTQPKLTDFSFN"
     gene            13996..14364
                     /locus_tag="ACF3T5_001679"
     CDS             13996..14364
                     /locus_tag="ACF3T5_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632282.1"
                     /translation="MGKINLNQIYTAKEMSERIGKNRNYLSQAYRNNKHEILKNFNYR
                     KIGGTIIFSDNPNNDLSQLITAKEASQLLGKNDEYFAHIYKRFPHRLEGIDHIYTGKT
                     LFLTKESLEAFKKKMNGNVR"
     gene            14424..>14686
                     /locus_tag="ACF3T5_001680"
     CDS             14424..>14686
                     /locus_tag="ACF3T5_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFD3632283.1"
                     /translation="MKKLALLTLFSTALLVSAPLVSFADETASSSDVTILADDNPVVP
                     VEPTDPTTPVDPVDPVDPVDPVDPVDPVDPVDPVDPVDPVDPV"
BASE COUNT         5459 a         2339 c         2722 g         4166 t
ORIGIN      
        1 atgttaagta aaattagtag tgaacgagat atgcttacaa aggcaaatga aattttagac
       61 aaacaagcag aaagattctt caaaaaatct tatccaagtt tgaatattga caaattttca
      121 aatcacgaag ttagagcaat ggttaatgaa accatattta gaaaacagtt attgaataaa
      181 gatcagttag cagaggtcat ttacaatgaa agagtagtag aaaaagaaga aagtaaaaag
      241 atttttaaag aaaaaccatt tcaaactagc cgttatcttg attcaaaaat taaacaagtt
      301 gaagatagta taacaaaaga aaataaccct gaaagaaaag aaattttatc aattaagaaa
      361 gaaaaactaa taggaataaa acaaggattg atagaatatg ttcaatcaga agttgaaaga
      421 aaatttgata aaaatgtttc aatagattca gtcatagaag gcgaaatgtt acttgcaaaa
      481 gctgactatt acaaaacaac tgatttttct aaagtcgaag gagttgctag attcagcagt
      541 gaggaaatta attccatgtt ggaacaatca aaaggcttct taactaatat tcagacggtg
      601 aaaattccta atgattgtca aggtgtattt tttgttcaag atagcatgaa acatattgat
      661 gaactaagcc cattagcaaa acaaaatctg aaaaaggttg ttaatcgcaa tgcttattta
      721 cctgattctg ataagataga attaagtaaa gaaattgaaa ataccaataa agatcaatcc
      781 caagaattgg ataaagacgt accagaaaaa aatgaagtga ctgtaaaaat gttccaattt
      841 gcgaagtcaa ttaatcgttt gttgagtggt aaccaactac agaaaaaacg aaacctagac
      901 aaattgatta agcaaacaaa agcaaaagaa aatcaatcat tacaaaggaa tattcctttg
      961 cgataaaata aaaacaagag gtgtataaaa tgaaaaaatt tatcaaagat acaaagttta
     1021 agcttggaag tgcggttgtt gcgttgggta cattgtttat tactgatcca gtgtttgcag
     1081 ccactgatcc acaagcgaaa ttagttcaag cgggtaacac tataaaaggt gttttaacag
     1141 ccttaattgt tgtagttggt gggattgctt gtgcgaagat tgttattaaa tacttgccgt
     1201 ctattgatga cccacaagaa aaaaatacca tgtataaagc cttgggaaca gccttgcttg
     1261 ttacggcatt aggtggggcg ttggtttggt tagtaccttg ggcgtatggc ttacttgctt
     1321 aatagagaag ggagttagtt atgaatagcg atcaagtgaa acaagcccta ttagatttgt
     1381 taaatgcaga cactgaaaaa gggcggactt ggttttttcc gtccaatgta tctgatcggt
     1441 acacagtcat tttagggcta gatttaaaac aatcagcaaa agcgatcggt acggcattaa
     1501 taagcgtgct attggcaatt cttattttcc gtagcacagc cgtttttcct ttaattatct
     1561 atgtcattgt tggtttggtg tcatttggtg gtgtatgggc gttttatacg attaaaccaa
     1621 ttacagaccg acctaacatt tctatatctg attttatgaa gcaaagaaaa gacttttcta
     1681 aaagaccaaa agtctattac aaaaagccaa aagaacgagt gtaaaagaga ggtgatttca
     1741 ttgtttgatt ttctaaaaaa aagttccaaa agtaacgata ataaaaaaag cgatacgata
     1801 aaagaaatga tttattggga agatagttcc cgatttcaag gcgtttttaa agactttttt
     1861 gtcgtttatc agccagaaaa aaagcaattt agccttgtga gtatgctaaa agttgacggc
     1921 ttaaacgttg atacattgcc agtatcagag caagaagggt taaacgaaga ttttggtgtc
     1981 tttctatctc aaaacgttct atatgaaccg cagatcactt ctaagaatgt accagtagaa
     2041 attgacgatt ttgtagaagc ctgggggaat acagtagaaa attatcgcaa aatgccaggg
     2101 cataacgaag ctttattaca actaaaagct agctattatt accattacag aaatttagca
     2161 agtaacatgg aaacttcaaa gaaacaacat tttgtaatta attctgaacc aatttcaaag
     2221 gaaacatacg acagtttgga attgtcttat caagtgttac gtgataagac aagaacaatc
     2281 agaacagctt taattgcatt tttaagtaaa tatgattgtc aggttgaaat ttgtacgatt
     2341 ggcgagatga aaaaggtttt gaatagttag gagcgttaaa atggagaaga taccaaaaga
     2401 gaaaattatt gtaatacccg aagttgatac ggacgttgta tctgatttag caccatttaa
     2461 ctttacagta gaacgtgaca aattattgat tgatgattca tacgcagttc cctatgtcat
     2521 tacaaaatac aataataaac caagaggaaa ttggtttaat cgtattcgta aaatgagtgg
     2581 agatataacc atatctcatt attacactaa agcaaacggt aactcattga atgattatta
     2641 caacagaacc attaagaaca agcaagcaga gatcgatcgt tcgcatgatc cgttgacgat
     2701 tatccgttta gaacgtgaaa tgaaaattgc acaaacgcag ttagaacaag ccgttgacga
     2761 aaacacttct tatctttatt tatataccta tgttttgatc aaaagtaagt ccgaagataa
     2821 actgaaaaaa ttgtgtgaag attttgaaac acgttgtatc gcaagtggag taaaagcctt
     2881 gattccatat actatgattg ataaagcgta ttggagtgcc ttacctttac aatctaatga
     2941 agtgcctgaa tacacgtaca caatcgctaa ttcaatcagt gcaagcagta tttttccttt
     3001 tgatgataat gaattaagtg tatttactaa aaatatgatt attgagggaa ttaataaaga
     3061 tactgaaaat atcgttagta ttgattacac caacagaaaa ttagtagtca atcgtaataa
     3121 attcgttttt ggtttatctg gtgggggaaa aaccacttac ctaacgtcag actatttgaa
     3181 aaaatatgct tttgctgata actcaacaga actaaaacac agaattgttt tatttgatcc
     3241 cgaagatgaa caaacagaac gtgtacgttc tctagggggc gaaataatca atctttcgtc
     3301 tatgtcagat gttcgtatca atccgtttca aatctactca cgcaatacac cagatgtgga
     3361 tttaaaagaa tcgttatccg attttgaaga ggacgaactt gtagaaaatc ttgaaataaa
     3421 gcacaaagat tttgaaatga ctgaaaatga tattgataaa gaaatcagta aacgaatgaa
     3481 tattttaacg ccatatttcc taatggtgga tcactctttg actgacagtc aattatctat
     3541 tattaagtta gaagcgaaaa aatgctatac cactttatac gaaaagaaaa acttgtcaaa
     3601 aatggaaaac accgattttc caacattttc agacttagaa agtcgattga aagcattaga
     3661 agaaactgat cccaaacgat acaagcgaat tgaagatttt atttattcat tagaagattt
     3721 tacaatcgga agccgtacca tatttaacgg tcatacaaat attgacttaa gcaatccgtt
     3781 aatttgcttc tctttgcgag atttacagac cgaagaagga atcagagatt tagcatacct
     3841 caacagcttt agttatctat ttgaagaaat aaccaacaat ccgcaaattg taacgtctgt
     3901 ttatgcagat gaatttcact ttttactgaa aaataaaatt agtgcagatt ttttcttcca
     3961 ggcttacaaa cgctttagaa aatataacgc cgattgtacc gtatcaaccc aacagattga
     4021 tgatgtatta aaagcacctg ataatatcgg taaagcaatt attgggaata gctttacaaa
     4081 agtgttcttt ggacttgatg aaacggaagc gcaaggtatc tcaaacgagt tgaaacttaa
     4141 acttacaaaa aaagaattat ctttcattac ctcaaaacgt caaggggaag ctttactctt
     4201 tcatggaaca aagagggcta aaattaaagt cgatttgacc caggaagaaa tgcgtttgct
     4261 taacccaggc ggatatgaag atatttacgg cgttagtccg aagaaagaga tcaattggtt
     4321 gttaagatcg aaaattcaat agaagggaga aaaaaatgaa atacaaaatc ttgaaaaatt
     4381 tacaattcta ctatcaagag aatgtcattg tcgtccaaat aaacgaaaaa tatttgacga
     4441 atcgagaaca tatttttgat gtagaagaaa gtgaacaata ttttgttgat gtcgaggaaa
     4501 ttttaaccaa agacggaaaa ctagaaattg tttatcaccg tcctaatggc tatacaccac
     4561 tacttgattt aaaagaatat gctgattttt ataaattgga tatagtgaat cgattacttg
     4621 aaatgaatgt actagaaaaa acaaacacct atctagcaat gcaaaatatc ctactcaaag
     4681 atacacgtga cttgcttttt atttataaag cagatcattt tggtaatttg ccttactcaa
     4741 ctaaagaaga attagagcag tggaaaaatt ttatttgtag tttttttggt aaattttcgc
     4801 ttgaaaagta tgagaagaat cgtattgaga ttctaacaaa agaaaaaaat tcatttttaa
     4861 atgatgtaga agcagttgaa agtttggaat cattaagaga tttaataaaa aatcgactaa
     4921 ccgaagaaca aaagaatttc ttttctgctg aattacagga taagaaagca aacgtccgaa
     4981 aaattcgcag aaataaaagc ttaaaaattg cgttagttgt aggtgttatt gcgttatatg
     5041 gcggtacggt ttttcttatg aaagtaaatg agaaaaaaca agttacggct acacagcaaa
     5101 gcgcagaaac agagatcact attttaaata agattattga taatgatagt gagaatatcg
     5161 aagaagatat gcaaaaactc aactatccta agaaaaaaca agttgatatt tacgtgaaac
     5221 ttggtgacta cactaaggct tatgaacttg ataaaaagtc agataaaaaa attattcaaa
     5281 gtctgtacaa acaaggggaa accgaaaaaa tagaaaatct tgatttgcca ggaagcgact
     5341 atttagcaga cttcaaaaag attttagcgt atgacaattc aacagatatt gagtacctgg
     5401 ttcagacaag taccgataca acaattgttg aagctttaat cgatcaagcg gtaaagaaaa
     5461 aggatattcc aacagtaaaa aatattcgtc aagtatcaat tacacaaaag aaattagcaa
     5521 tcgatcctaa acgtcaaatc agcatgattg acttattgat tgaaaataac agtgaagaac
     5581 tagaaaatat atataaggat aattctttaa atgacgactt gaagaaaaaa caaaccaatg
     5641 acttgttaga agaaaacaac acattgctta gtgaaaagat tgaattaaca aacgctgaaa
     5701 aagattagga aggttggtga tttcatatca tgggagcaac agctgctaaa ctgcttattc
     5761 gttatcgaaa accgatagcg aaagcaattt taggcttttt cgtctttatc attgtaatct
     5821 tccttcttat ttttaataac gatcaaaccg cccctagtgg cggactagca acagaaaaca
     5881 aaaatttaag tgaatctgtt ttgaggtaca aaagtaccgt tgaaaagtac gctagacaat
     5941 atgacggtat ggaatatgta ccttatatct tagcactcat gcaaattgaa tcgggcggga
     6001 atggtggcga tcctatgcaa tcaagcgaaa gtttaggatt gccccctaac accattcaag
     6061 accccgaacg aagcatacaa cgaggagtag agttttttgt agaaaatatg aaaagcgcta
     6121 taaaccgagg atcagatata aaaacagctc ttcaagcgta caattatggc ggtggttttg
     6181 ttgattttgt agtgaagaat ggcggaacgt attcttttga gttggctaaa caattttcta
     6241 ttaatatgtc aggtggcgaa aaggtacctt atgtaaatgc tttatctact tcaatgggct
     6301 acaattaccg ctataactac ggaaatatgt tctatgtgcc aaaattactt gaatacgtta
     6361 acgaaagcca ggcaagcgca gagggcgttc aaaacaattc taataatact ggcgctgttc
     6421 aagttgatgt acctagtgag tacaaaaata aattacgtta tccaagatat gacggacaca
     6481 actataacac aagcggtagt tatccatttg ggcaatgtac ttggtacgct tttaatcgta
     6541 tggctcaaat aggaaaacca gttgatgatt ttatgggcaa tggtggcgaa tggggctata
     6601 aagggaaagc tttaggctat aaagtaacaa ataagcccaa agtaggtacg gcaatttctt
     6661 tccctccagg ttctttcggt tctgatccag tttacggaca cgttgccttt gtagaagtcg
     6721 taaatgcaga cggtacgctt cttgtatcag agtgtaatgt agtaaaccca ggaagtggaa
     6781 caatcagtta tcgagtagtg ccagcttcta ttgcaaacat tgcaagttat gttgaataag
     6841 gagagaaaaa aatggagttt gacaaaataa aagttagttt gtttatctca acgcttgctt
     6901 tacttgtggt taccataggc atgattgttt ttggaataaa gcaaggcgaa cagatccgtc
     6961 acttaaaaag tgatttaaaa agcgtacaga cggaactagc agacaaacaa gcggaacaaa
     7021 acactaacca aagcgaatcg gaaaaaatta tcaaagaatt ttacaagacc gtttataact
     7081 acgaaaaaag ccaaaaagaa atatctatga caacggttaa agaattagcg acagacaacg
     7141 tttatcaaga attacaaaac gagatcaacg taaacaattc atatagccca caacaaaaca
     7201 cgattcaaaa gtcttcagta aacgaaaatg aaataaaaat tcttgcttat gcaagtaagg
     7261 ataattcaca gcaatatcta gtaacagcac cgattcacca ggtttttaac ggtacaaaaa
     7321 atgattttga aataaaccaa attatccaaa ttaaaaacca acaaattact caaagaacga
     7381 ccattcaatt aggagaggag tagaagcatg gcgaagaaaa agcaagagca agtaaaactg
     7441 attatcctag ttatactcta tctactatta ttaccagtaa cattgattgt gtttaaatta
     7501 ttacctttta tcattgcgaa tgatacaaat attgaattga gtacctttgt tacaactatt
     7561 ttaaagcacc cgatcaatac ttttttagag atcattcaat ctaattacat gattccgttc
     7621 ttcattgtac aaatcattgt tttaattatt ttatttgtct ttcttcaccc taccaaacgc
     7681 caaccgtttg aggttgtcgg gaaaacaaac cctgttcatg gatcggctta ttggggggaa
     7741 gaatcagaaa taaacgcacc aaaaaatgtt catttgatcc ctgaaaagtc aatgaaaaag
     7801 atattagaaa agtcaatgag gagggggaaa aatgagtagt ttgttagcag aatcagacgg
     7861 tttaatctta gggaaatttc aaagcggaaa tgttgtgatc caacccgaag atagcaaaat
     7921 aagcaatcga aatatttttg tagtaggtgg tccaggatca tttaaaacac aaagttatgt
     7981 tttgcctaat gttgtaaata atcgcagtac gtcaattgtt gtaaccgatc caaaagggga
     8041 aatttacgag ttaaccaatg aaataaaaaa agcccagggc ttcaaaacgg ttgtcataaa
     8101 ttttaaagat tttctactaa gttctaggta caatccatta ctttacattc gcaaatcaaa
     8161 tgatacaaac aagattgcga acgtcatagt atcagcaaag aatgatccca aacggaaaga
     8221 tttttggttc aatgcacaat taaacttatt aaatacactg attaaatatg tttattttga
     8281 atatgaacca agcgcaagaa caattgaggg tattttagac tttttagaag aatttgatcc
     8341 acgctataac gaagaaggtg tatcagaact tgatgaacaa tttgagcgtt tgccagacgg
     8401 acacgaagca aaacgcagtt actatttagg cttccgacaa gcacaaagtg aagcaagacc
     8461 aaacattgtt attagtctat tgacgacttt acaagatttt gttgataaag aagtagcaga
     8521 atttaccagt acaaacgatt ttttctttga ggaattagga acgtcaaaaa tttgtttata
     8581 cgtgctaatt tccccattag atcgaacttg ggacggctta gtgaatctct ttttccaaca
     8641 aatgtttaca gagttatatt ttttagggga taagcacaac gcaaaattgc cagtaccttt
     8701 agttatgtta ctagatgaat ttgtcaattt gggctacttt ccaacatacg agaattttct
     8761 tgcaacatgt cgagggtatc gtatttcagt tagtaccatt ttacagtcat tgccccaagg
     8821 ctttgaacta tacggagata agaaatttaa agcgatcatt gggaatcatg ccattaaaat
     8881 ttgtctaggt ggtgtagagg aaacaaccgc tgaatacttt tcaagacaag taaatgatac
     8941 aacaatcaag gtatatacag gcggaacaag tgaaagtaaa acgtccgcaa aaacaactaa
     9001 ccgaagcgga agtaagtcag aaagctacgg ttaccaaaaa aggcgattaa ttaccgaagg
     9061 ggaagttatc aatttacaac aagaagaaaa tggaagaaaa agtattgtgt taattgacgg
     9121 taaaccttac atgttaagaa aaacgccaca atttgaattg ttcggaaatc ttcttaaaaa
     9181 gcatgaaatt tcacaacaag attatataag cagtcaaaca gaatttgcca aagaatacat
     9241 tgaagaattg gaagtacagc acaagcaaag aaaagtacaa ctcgcaagcg ccccagtttt
     9301 tcatgagaag aaacaagaaa atgacttaaa taaaaaagaa atcaaattac ctgaacaacc
     9361 gatcgaatca gaagtaacag aagaagtaga agaaaaagaa caattaatga gtgatattct
     9421 aagtagtttt gatgaaggta tagaggaaaa cacaaccaac aatgacaaca gcgaattacc
     9481 attttaaaga tcggaggaaa tcaaatgaat ggattgaaag aattttttgg gaatatccca
     9541 atgtttcaaa aacaagttga gcgagaaatc gaagaaaaag ggattaaacc tagcgagggt
     9601 attaaaaaaa tggaatttag ccgtcaagtg cttagaaaca tagtgattgg tgtcttgttt
     9661 attggcattc ttgcctttgt tggtatttct tattttaaca ataagaatca accaccagta
     9721 gcaccaaccg ccaccaccac aagcaaagaa agtaaccaat cggaagcaag cggagaagct
     9781 acagaaaaca gctcacaagc cgttcaaggg tcaagcgacc atttactcaa attaagtgcc
     9841 aaagaacgag cagacgaagc gacagaagct tttgagagtt ggtacaagtc attttcaaaa
     9901 ggtgacgtga ttttagagat caacaaagag ttattgaaag aaggatcagg cggaacaagc
     9961 ccgattgagt tacaaacaaa actcattgat aacttaaaag ccaagtttgg cgataaagtt
    10021 tcagatgatt tctattcagg tttacaagca agctttaatt tcaatccagt aatcgttgac
    10081 ggtacaaaag ggctaacgat ttccaagcaa aatgacgatc aaagccaatg gttacggact
    10141 tggttcttag acactgaaaa aacagcgcaa aataccaaaa ttatcctacg caatgatttt
    10201 tcttatgagt gggcagattg gagaaacaaa ggacaacatg atgagaaagt aggatcaatg
    10261 tttacacaaa ttgattgggc taatgattta agatatgaag ttatagggat agtaaccagc
    10321 aaacaagaaa aaaatataga aaatattggt ggagtatttg tagttatgca atacaacgaa
    10381 aaaattggaa agtggcaagt tagcggaacg attggtggtg taatgtaata tggacgtaat
    10441 tgaccaaata aaagaaaccg gatcagacat tttaagttct gccggtgatg ccatatcagg
    10501 aatatttgaa ggcgtaaaag agagtgtaga tagtgttttg cacccgatcg atagtttatt
    10561 aggcagttgg ggaagcgacc ttttagaagc aagcatgaaa attgcgaata agacaacttt
    10621 tgatattcca aatataaaat taattagcac cattaccaac gtgtttgtct ttgccactac
    10681 gacctttgca atttgtattg tgttgtataa ggtcattgaa gcgcaaatac aagcttctaa
    10741 tgggagtggt gacgcattga ccgctaatat tgttcaaaag ctgttttact cgtctattgc
    10801 cttagcaacg ttgccctggg caatgaactt ttttatcaaa aagattgttg caccaatcgg
    10861 ggaatatgtg attcaacaag tcgctaatga tttgaatgtg gaaacgatcg gcgaacgatt
    10921 gcgtaatttc ttagtatcaa atttctttaa tggtggcgtt agcgccgttg ttctctttgt
    10981 tttcgtgata ttctttgcct tttccattgc caagtatttt gtagcgatct gtgtctttta
    11041 tgccgatttc ttggtactga ctatgttaac accattagtt gccttatcgc ttttaacaga
    11101 cgaacaaaat tactttcaaa tatggatcaa agaactgtta tccgaagcac tcacaatgct
    11161 tattaagctt attttgtact tactcatgat tacactcatg attgctgaaa aatacacgtt
    11221 accaaacttt atgttaatgg ttggttgtgg cttgttgatt atcaaaacac cgtcagccct
    11281 acaaaatatg tggtattcaa ccaaagtgtc aaaaggtggc ggacttggaa acttagcaat
    11341 gatggctatg tttagaaaat aagagagact aactatatag agtcaagtaa aaattagcca
    11401 aatcaaaaaa tattttataa cagataattc cgaaaaaagg gtacaacaaa taagcttctt
    11461 accattagtt tttttgaatg ttcaatagct aatcagaaac aattatataa ggctcgtttt
    11521 tagtcaaaat agcatgaatc acgtaacaaa gcttacgaga aaccgcacct aaggcagtta
    11581 gatgatgttt tccttcggaa acttttttct ggtaaaacgc actaaaaaca ggatcacatt
    11641 gagaagctac taaagccgcc gaaaacaagg cttttcgcaa atatggagac cctcgtttac
    11701 tcataacatt atgcgttgcc tcatattgcc ctgattgtgt cacggaagcg tctatcccag
    11761 cgtaagcaac gagttttttc gggttagaaa atttttgaat atctccgatc tcaccaagaa
    11821 tagtcgcacc gtttatagga ccaattccag gaattgtgag aataacagaa tttaaagtgt
    11881 ccattatgtg cttgatttct ctttccgttt cctttatttg gctttcaatg aattttaatt
    11941 gttcaatcat tgaacgtagt tggaaggtga acgcttcttg agcaaatttt actccaaagg
    12001 agttagaagc tgcttgcttc aattggtccg ctttttctgc cttgattcgg ttcctgctta
    12061 actcagctaa gtattgggcc aaagtttctg aggaaatgtt ttctaagtcg atcggggcac
    12121 taaacgccag caataactct ttagaagctt ttccgaaaac gccctgcttg gtaaaaatcg
    12181 tcgcgtattc tggaaagact tgatccaata cggctataat ttttcttttg aagtcgctcg
    12241 ctgtacctac taaataagtt cggtaacgag aaagttgttt caaagaaaaa acattttcat
    12301 cggataagac cgtttcgaca aatgagccat agcgcattaa gtcagcaatc aaaacagaat
    12361 caataatatc gttttttcgt ttccgtattt ctgtaccttt acgccatccg tctgtttgaa
    12421 ggggattcac aacgtgaatc aaataatctg attcgtgaag gtatgaaaag atggacagcc
    12481 aatagtgccc cgtggcttcc atccctataa caaagtcact gggtgctccg gaatagtcga
    12541 ccattcgctc aattaatgac tctgctcctt cagtggtgtt aggaaatgaa aaacctttta
    12601 gtaatacctt tccttcctcg ttcatgatag aagcaacgtg agtgcgttta ccgatatcaa
    12661 tgcctaaaaa gtacatgtat atctgacctt tcaaaaaaag aattttggat tgaaatccac
    12721 tcaactgaaa agcgtcacta ccttgtacga gatacggagt aaagtaagga ctttcaacat
    12781 ctaactcata cgcaatccac ttatcagaga gaggagccag tctctcgagt gcgggttcaa
    12841 tgacccaagg ggggaacggc ttagcctctc aatccaataa atatattatc tcaaaagaaa
    12901 tgagttaata ccaaattaaa ataacgatgg ttcatagggt tatccaatca aaaccggaag
    12961 gttaaaaccc taactctaat atacaagggg gataacgatt gtcttattat tttgaaatac
    13021 gaattattct tcccgaagaa gaaaaacaat ttttgaatcg aaaattaagt aaaagcgaat
    13081 tatccgaagt cacacactat ttacaacaaa agacaagtcg aggaattcca gttaaatttc
    13141 gtgtaggtat ttttagagta gaagatcaaa caaaaattat gtctgttacg ctgaatacaa
    13201 aaaataccaa agaaacggac gtaattaact tgttattaaa tcgagtaaca gatcaacacg
    13261 ttttagttta cttgaatgaa ccaacagaac caacgcttaa tacgcaagaa ttaaatcgtc
    13321 aggagttaaa aacgagtaat gaacgccaag aaataccgca gacagaaatt ccaactgaaa
    13381 cagtaaatga acctagtgtt attaaggaaa tatcgaaaaa gaaccaagca aaacaaacga
    13441 atagtagaaa agaatcacta tcagaatcca taaccaagaa aaatgttccc aaaattcatt
    13501 tgttcatatc tattttgact ctttttattg tattattgat aggaataagt gttattcaac
    13561 aagtacaact tcaatcagtc aaaaaagaga gcgaattatt gaaagaacaa attgaacgtg
    13621 tcaaagaaac ggatatttct caatcgaaaa tagatacttt tggaagatac tttttgactt
    13681 actatttttc acaggaaaaa aatcaagaaa attatcagtc aagtttaaga acatatgtat
    13741 ccgaaaaggt agatatagct gattggaaag cgttagagaa aatgcttaag agtgtcaatt
    13801 actatggtag tgaacaaaca aaaaaagggt atagcgttga ataccttcta aatgtttcag
    13861 tagacaatcg tagtaaaatg caaaaaatca cttttgaggt tgaaccaact aaaaacggct
    13921 ttcttgtgac aactcaacca aaacttacag acttctcttt taactgaaat acgctaaaaa
    13981 gagagtgctt aaacaatggg aaaaattaat ttaaatcaaa tttacacagc aaaagaaatg
    14041 agtgaacgga taggtaagaa tagaaattac ctatcccaag cttaccgtaa caacaaacat
    14101 gaaatactta aaaattttaa ttatcgaaaa attggtggaa caattatttt ctctgataat
    14161 cccaataatg atttatcgca attgattaca gcaaaagaag caagtcagtt gttaggaaag
    14221 aatgatgaat actttgccca catttataaa cggtttcctc atagactaga gggaatcgat
    14281 cacatttata caggtaaaac attattctta acgaaagaat cgctagaagc ttttaaaaaa
    14341 aagatgaatg gaaatgtcag ataaagcata tttatgatat actatttatg tacacaaata
    14401 ctaattttta aggagattat aaaatgaaaa aattagccct cttaacacta ttcagcactg
    14461 cattattagt atcagcaccg cttgtcagtt ttgcagacga aaccgcaagt agcagtgatg
    14521 ttactatctt agccgatgat aatccagtcg ttccagtaga accgacagac ccgactactc
    14581 cagttgatcc agttgatcca gttgatccag tcgatccagt cgatccagtt gatccagttg
    14641 atccagttga tccagtcgat ccagttgatc cagtcgatcc agtcga
//
LOCUS       DAXLPI010000062        14643 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXLPI010000062 DAXLPI010000000
VERSION     DAXLPI010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.062"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(32..310)
                     /locus_tag="ACF3T5_001681"
     CDS             complement(32..310)
                     /locus_tag="ACF3T5_001681"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632284.1"
                     /translation="MENKVGYVRYNFFSSTPIMKDFASFNEALAVELSHDRERIHYEK
                     HVIINDLWNTEKTDLLALPDTDYPVFKEIEVKANKYNEIKQNKITNLS"
     gene            complement(425..1033)
                     /locus_tag="ACF3T5_001682"
     CDS             complement(425..1033)
                     /locus_tag="ACF3T5_001682"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632285.1"
                     /translation="MTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLLPQP
                     KARKKTGMMYTEKWGGIVSLWLSEDAKLPNKKRRTAEAMTTALKADGFPGSYRTVCVF
                     IQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPFSNA
                     GFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVT"
     gene            complement(1437..2261)
                     /locus_tag="ACF3T5_001683"
     CDS             complement(1437..2261)
                     /locus_tag="ACF3T5_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD3632286.1"
                     /translation="MEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAGANKKLV
                     YNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLKILTTFL
                     YVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKFVYDDFS
                     DELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGIPYINNL
                     GLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQHLEIS"
     gene            2604..3020
                     /locus_tag="ACF3T5_001684"
     CDS             2604..3020
                     /locus_tag="ACF3T5_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632287.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="ACF3T5_001685"
     CDS             3017..3487
                     /locus_tag="ACF3T5_001685"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3632288.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="ACF3T5_001686"
     CDS             3501..4274
                     /locus_tag="ACF3T5_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFD3632289.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="ACF3T5_001687"
     CDS             4288..5100
                     /locus_tag="ACF3T5_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFD3632290.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="ACF3T5_001688"
     CDS             5154..6119
                     /locus_tag="ACF3T5_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFD3632291.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="ACF3T5_001689"
     CDS             6256..8979
                     /locus_tag="ACF3T5_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFD3632292.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="ACF3T5_001690"
     CDS             8992..9624
                     /locus_tag="ACF3T5_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3632293.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9832..10056
                     /locus_tag="ACF3T5_001691"
     CDS             9832..10056
                     /locus_tag="ACF3T5_001691"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFD3632294.1"
                     /translation="MQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEVI
                     PENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10158..10391
                     /gene="relB"
                     /locus_tag="ACF3T5_001692"
     CDS             10158..10391
                     /gene="relB"
                     /locus_tag="ACF3T5_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFD3632295.1"
                     /translation="MSQSTITFRIPDDEKELVLEYAKAHNSSLTELYRNAVLDKIEDE
                     IDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="ACF3T5_001693"
     CDS             10381..10650
                     /locus_tag="ACF3T5_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFD3632296.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(10829..12130)
                     /locus_tag="ACF3T5_001694"
     CDS             complement(10829..12130)
                     /locus_tag="ACF3T5_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFD3632297.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(12359..13906)
                     /locus_tag="ACF3T5_001695"
     CDS             complement(12359..13906)
                     /locus_tag="ACF3T5_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFD3632298.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(14008..14361)
                     /gene="tnpB"
                     /locus_tag="ACF3T5_001696"
     CDS             complement(14008..14361)
                     /gene="tnpB"
                     /locus_tag="ACF3T5_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFD3632299.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(14351..14545)
                     /locus_tag="ACF3T5_001697"
     CDS             complement(14351..14545)
                     /locus_tag="ACF3T5_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632300.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT         4668 a         2481 c         2835 g         4659 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgcgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt atggctcttt gtcaaatagg actgatagag tgagataaga
    10801 acatctaagg tagaattcct tttctacttt agatgttttt tgattcatag gtaatcagat
    10861 tatttagtaa gaaaattctg gtcttcattt tccgaaagct tcgaaagcca taagcgactc
    10921 gtttcaatgc tttgatgtgg gtatgcagat tttctatttt ggcatttgaa taaggcaatt
    10981 cgagggctaa acgaatgccg gattcatagt ttataaaagc tttttttata gaatgcagtt
    11041 cttttggaac ggaattgggc atcgttctta caagtttaaa gaaagtgtcc gcctctttgt
    11101 cgtgaaacgc ttgaagaatt tcttgatagt aaaaataggc gattcgtaat tcgtcagaga
    11161 tcgaaagcag acgatccacc acttcacttt cggtgagata cgcccaacca aaattgcgcc
    11221 atttggtcca tttgtcataa acaagcttgt cctgtcgttt gatcagaagc cgagacaacg
    11281 cctttaattg tcgataatat ttggcttgtg ttggatcgtt tcgatcaaag cttttcatga
    11341 ttcgtacgcg aatatcctca aatttacgaa tgagatgttt gacaatgtga aaacgatcca
    11401 caacgagttt tgcctttgga aaacattcct ttatcagaga ggcataagaa acattcatgt
    11461 ccgtcacgat gacctttacc ttcaatcgtg cgcgtcgttc aaattgcata aaataagaaa
    11521 cgagagcctt tttcttccgt gaaggaagaa tatccagtaa ttttccagta tcgccatcca
    11581 tacaagaaaa ggagtaggga ccgacttgat ttttcagtga acgaaattca tccattaata
    11641 aacaacgcgg taaccaattt ctttttactt ttgtcatggg gatcagagaa gcgagtgtcc
    11701 gagaaacggt cgaccaagaa acgccgcaat gcttagcaat tgtcgtcatt gcttgttttt
    11761 cagttagctc ttctaaaatt ttatagcgca cttgtttagc gataaagcag tttctatcga
    11821 caatgtaggt attcgccgta aaataggtgt ggcagccacg acagtggtag cgttgtttcg
    11881 ctaaagcaag ataagtcagt tggttcgcac atttattaag ttgaatgagc gattttttct
    11941 ttccgttctt aacgatcatg ccttcatgat tcatctgttt acacaaagga cagcgacgaa
    12001 tttctttact taacgtcgca gaaacaacaa gactcttgat attcttaatg ctcttcgatt
    12061 ccacatttgt gatgaaaatg tttttatctt tgattccaag tagtagtttg atatgatgaa
    12121 gatcaggcat acagaacgct cctttgattg tgttgtggtg attcaattct aacggagctt
    12181 ctgtatgttt tgttttttca tgctcaaaag ggaccgatag aatgattttc tatcagtcct
    12241 agaaattata gagccttttt tatttggtct tgaagaagaa aagtaagcgc cccatagaac
    12301 agtacctgta cgtcaaataa cccgacctat agaatagaat cgggaatgtt tggcgttttt
    12361 agttacgaga aaaatgttgt tgtacatcct tgttccaagg caacagttga tctaaagttt
    12421 ctgctgaaag tacgggaaga tttggtatct tatcaaatag aaactctaga tactttacag
    12481 gattcaatcc gttaagttct gctgttttat atacactcag aatgattcct gaagttcgtg
    12541 ctccttcaag acttgtggaa tgcaaccaat ttttccgtcc tatcaccagc tctttgatat
    12601 ttcgctccgc acgattattt gtgagttcta aacgaccatc ttcaaaaata ggttcaaaac
    12661 cacttcgtag tttacacgca tattgaacgg ctgcatccaa cttggatttt gcaacaggat
    12721 ctatcgttgc catccactca taaaaagcaa taaataatgg acgcagttct tgttgccgac
    12781 agcttaatcg cttctcagga gaaaagtctt tccatttctt ttctaggaca aaccatttat
    12841 ccaattgttt gactgcttga gctgcgggaa tatccgtgtt tttctttcct tttggaattg
    12901 cttcgaaaaa tttccgccga acatgcgcga aacaatagac aagtcgtaca ctttccacgg
    12961 cattataccc gctatagccg tcacagtgta aataccctgt aaactccttg agaaaagttt
    13021 tcggaacatc tccagcacga ctttcgtcgt ggtgataata aacgatggga tgctgactgt
    13081 cttttcctgt acaaaactgc cagtaatagg tatctgtttt ggcactttca atcacacgat
    13141 aacttgtttc atccccgtgt agaatctctt cttgattaag atagactctt aatcgttctc
    13201 ctaaagggtc taacgcgttt tgcactgctt taatatgcca attggtgata ttatctctcg
    13261 aaatatccaa cccatgtgtt tgtttccaat agttctcttg ccgataggca ggaactttct
    13321 gaccaaattt catccggatc gtttccgcca ctaaactatt ggaacctaag ctattagaaa
    13381 tcaaaggttt aggaacgatc gcttttttaa aagaagaaag tccttctttt tcgcagcttt
    13441 tacaacaata ggtatgttga taatgggtga gacaatccaa catagcctga tggaaacgaa
    13501 tttcttctcg tacttttgtc cgtccgatat ctttcatctg tgaaccgcaa tgttcacaaa
    13561 attgttcttc caaaggaagg agatgttctt tctctgttat aggtaagtgt ttgattttgt
    13621 ctgctttcgt tcctacaact cgttgatgtc cttttactgg aataatggtg gcagctgctt
    13681 ctggaacact tacagaaatt tcttcatcac taaaaagatt caattgccca ttcgtttcct
    13741 caaagagagt ctctttagag cgtccaaata atttgttggt taaataattg accttttcat
    13801 tcaatagttg gatttcagtt gtaagattct gaatggtttg ttgttgtgct tgattggttt
    13861 tatgggcttc ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc
    13921 ttgaatcgaa ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca
    13981 acacattttt tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc
    14041 tcgaggttga tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt
    14101 tcgaacttcg tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca
    14161 gagaagaaaa ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca
    14221 aaataaaaaa acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc
    14281 aagaccatca attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt
    14341 ttgaagtaaa tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat
    14401 tgttaatacc gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta
    14461 acttggcagc cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag
    14521 gaacaatctc atttaattta tacatagaag acctccagac ttcttgatag ctcaagtata
    14581 tcttgaggtg catttgttag taaggtactg ttctatgggg cgcttacgtt aaaaatgttt
    14641 tgc
//
LOCUS       DAXLPI010000063        14549 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXLPI010000063 DAXLPI010000000
VERSION     DAXLPI010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.063"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(14..580)
                     /locus_tag="ACF3T5_001698"
     CDS             complement(14..580)
                     /locus_tag="ACF3T5_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3632301.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(580..1536)
                     /locus_tag="ACF3T5_001699"
     CDS             complement(580..1536)
                     /locus_tag="ACF3T5_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFD3632302.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1536..2192)
                     /locus_tag="ACF3T5_001700"
     CDS             complement(1536..2192)
                     /locus_tag="ACF3T5_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFD3632303.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2562..4580
                     /locus_tag="ACF3T5_001701"
     CDS             2562..4580
                     /locus_tag="ACF3T5_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFD3632304.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4650..4856
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5007..7421
                     /gene="leuS"
                     /locus_tag="ACF3T5_001702"
     CDS             5007..7421
                     /gene="leuS"
                     /locus_tag="ACF3T5_001702"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFD3632305.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7769..9322
                     /locus_tag="ACF3T5_001703"
     CDS             7769..9322
                     /locus_tag="ACF3T5_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFD3632306.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9703..11487)
                     /locus_tag="ACF3T5_001704"
     CDS             complement(9703..11487)
                     /locus_tag="ACF3T5_001704"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632307.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNFAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11502..13223)
                     /locus_tag="ACF3T5_001705"
     CDS             complement(11502..13223)
                     /locus_tag="ACF3T5_001705"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632308.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLQIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13323..14084
                     /locus_tag="ACF3T5_001706"
     CDS             13323..14084
                     /locus_tag="ACF3T5_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFD3632309.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IRERKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4721 a         2686 c         2899 g         4243 t
ORIGIN      
        1 ttgcgttttt tatttatctt tttttctcaa tacaatataa aataggcggc tggttctttt
       61 ggttgataaa ccgataattc agcacattat atgtttcctg tggcagtttg gaacaaaaag
      121 aatcaaccat atccagttct ttttctccac cttcatgacc atagtagacg acaaggatca
      181 atcgtccgcg aggcacaagt cgtttcaata tttcttccat agctgtgcgt gtagtctctg
      241 gcatagtgat gattgcttta tcactttttg ggagataccc tagattgaaa atccctgctt
      301 tgagattttg ttgttcagct atcacttctc ctaacgtttc gtggccttgt agaaacaatg
      361 agacacgttc ttctaactga cgctgttcta gcctttcacg agtagcatcg attgcttgtt
      421 gctggatatc aaaactatag acatgaccag tttttccgat gtgttctgct agaaaaaccg
      481 tatcgtggcc attgcccatc gttgcatcta cgacgtgatc ccctggttgc aagatttctt
      541 gcagtaatgt atggctgaaa tgcaaagcag tttgcaacat cagacgctca cctttttccg
      601 tacatcatat tttccttgga agctatctct gcgaagaagt tcttcatcaa ttgcattcaa
      661 gacttcccat tttttcaagc tccacattgg tccaatcaag gaatcccacg gcgcatcccc
      721 tgtcaaacga tggatgatga tctcttttgg tatcatttcc agctggtcgc aaatgacatt
      781 gacatagtca ggacggctca ttaattgaag gcgaccttca tgatagtccc gcaacattct
      841 cgttttgcgc atcagatgca tcaaatggag cttgattcct tggatatctg aatctaaaat
      901 ggtgcgtctg acattttctt tcatcatttc taaactttcg cctggtaatc cgttgatcaa
      961 atgcgtacaa acacggatat tatgtttgcg taatttagcc acgccatcta aataagtttg
     1021 ataatcatgt gctcggttga tcagttttga agtttcttca aaagttgttt gcaatccaag
     1081 ttctacccat aaatacatcc gctcgttcag ttcagcaagg taatcaacta cttcatctgg
     1141 cagacaatcc ggtcgtgtac cgactgataa tcctacgaca cctggtaaat tgactacttg
     1201 ctcaaatcgt tctttgatta tttcaactgg cgcatgggtg tttgtaaaat tttggaaata
     1261 aacgatgtat tgttggacat gaggccattt ttgatgcatc atgtcgattt ctttctggaa
     1321 ttggattgga agagggtctt caggtgcaac gatcatgtct cccgaaccag atacactgca
     1381 aaaagtacaa ccgccatggg caacagtccc atctcgattc ggacagtcaa atcctccatc
     1441 gatcggcacc ttgaaaattt tcccgccaaa ctcatttcgt aaagcgtaat tccaagaatg
     1501 atatctttta tttccttctg aataaggaaa agtcattaaa agtccttctt tctgtaagag
     1561 ttatctattt gttttctttt ttctctatac actggataag tcaggcacag ccaagaaaga
     1621 ctgagtaagt aaccgccgat gatatcagta ggaaaatgaa cgcccagata aatgcggctt
     1681 atcccgacac ttactataat aaacatcatc agcaattgaa gcaatgtgcg taaggttctt
     1741 ttttgaacaa aagctggaag cagaaatagt aaagaaccgt aaagaatcat actgcctgct
     1801 gcatgtccac tagggaaact gtaacttgtt tcaactacta agtgcgccaa tgaaggacgc
     1861 tctcgcatga aaaatagttt gatcaccggg ttgatgattc cagcaattac aataaatcca
     1921 gaaacgaacc aaaatgtttc tgcccttttt ttcttatacc acatccataa agccgcacaa
     1981 gcaaataaaa tgatgacggt aagaggatta ccgaatttag tcataaagag ttggtagctg
     2041 ttcagatcag gataagaagc tctgacaatg cctgtaaacc actgatcgaa accagaaagc
     2101 cacgacagat ggaactttac gatccagccc aaaatgaaaa acaggacaag aaagaagcta
     2161 ccagccacct gaaatgatgt tttatatttc atacaattcc tcctttgaag caatgactta
     2221 ttatagcatt tttggtccag cgaaaaaaac ggtaaagaga taaaaccaat gtatgattcc
     2281 tctttgaata accaatattc ttataaaaaa aataactgta atcggaaaga cattttttgg
     2341 taatgcaaat gaaatgttag ggaaataaaa tcagtgtttt tcgaaatcct tttaaacaag
     2401 aacgtagacg taggaagaac ttgaaaatga acagaagttg ttcattacgg taaattacaa
     2461 tatgattaca aaaaacaaca aagctcttaa ataactttta ataaatatta agagaagtta
     2521 taattattaa taatagataa attgaagttg gaggatagaa gatggaaaac attgcaagaa
     2581 aagaacggag acgtttagaa cagaccaaac gttttcgaaa agcaaaacga ggggcagcaa
     2641 tcgtgggaac tgcgatggta ggctgttctg tcgctgcccc attagtacag ccggttcctg
     2701 ttcaagcgga tgaaacacct gttcagttcg gcgcaagaat caatacagcg gcatttatca
     2761 cagaaattgc tacttatgct caaccaatcg ctcaagccaa cgatctatat gcatcagtga
     2821 tgatcgcaca agcagttgta gaaagcggtt ggggcagtag tgcgttatct caagcacctt
     2881 attataatct ttttgggata aaaggcagct atcaaggaca aaccgtctac atggatacgc
     2941 ttgaattttt aaataatcaa tgggtaaata aaaaggagcc tttccgacaa tatccttctt
     3001 ttgctgaatc tttcaatgac aatgcctatg tacttagaaa tacttcattt ggcaacggtt
     3061 attactatgc cggtacttgg aagagcaaca cgaaatccta tacagatgcg acagcttgtt
     3121 tgacagggcg ttatgcgact gatcctggtt acgcaggcaa attaaataat atcatcgcaa
     3181 cttacggatt gacccaatat gatacaccag ctagcggaaa cgcaggcggt ggagcgacta
     3241 caggtggcaa taatactgga agtgcaggta gtactggcgg ttcaagcaat tctggtaata
     3301 caaactcagg ttcagcaact tcgaccactt atactgtcca atctggtgat tctgtttggg
     3361 gaatctcaaa taaattcggt atcacaatgg atcaattgat ccaatggaac aacatccaaa
     3421 acaatttcat ttatccagga caaaaactga caatcaaagg tagccaggaa aatggctctt
     3481 cgacgaataa ttccggtaac aataccaatt cttcaggaaa tgctggtaca tcaaacggag
     3541 gacaaacaac tggtgcgaaa tacactgttc aatctggtga ttctgtttgg aagatctcaa
     3601 atgatcacgg aattacgatg gctcaattga ttgaatggaa caatatcaag aacaatttcg
     3661 tttatccagg acaacagcta atcgtatcaa atggcggttc ttctaatgct ggagcaaata
     3721 ctggctcaaa tactgggaat acaagtggct ctagttcgtc cagctcaagt tcttctaata
     3781 cagcttcagg aacgaaatat acagttaaag ccggagactc tgtttggagc gtctcaaata
     3841 aatacggaat tacgatggct cagctaatcc aatggaacaa tatccaaaat aactttatct
     3901 acccaggaca acaactgact gtttcaaaca gtggatcaac aaacagcacg tcaggtaata
     3961 caggttcgtc aaccaataca aataacagca gttcagttaa tactggcagc agttcgtctc
     4021 aaggcactta taccgtgaaa gcaggtgaat ctgtttggag cgtggcaaat aaaaacggca
     4081 tcacgatgga tcagttaatc gaatggaata atatcaaaaa caacttcatt tatccaggac
     4141 agcaattgat cgttaaaggt ggaacttcgg caaatacaaa tacaggttca actacttctg
     4201 cagcgaaacc aaatacaccg aatacgtctg ctacgacaag tacatcatcc actggtaata
     4261 cgatgtatac cgtgaaagcg ggcgagtcag tttggagcgt agcgaacaaa catcatatca
     4321 cgatggatca gttaatcgaa tggaataata tcaaaaataa tttcatctat ccaggacagc
     4381 aagtgattgt gaagaaaggt tcatctcaat caacaaacca acaatcagtt actggttcga
     4441 aaacctatac tgtgaaatct ggagaatctg tttggagtgt tgctgacagt catggaatta
     4501 caatggctca actaatcgaa tggaacaata ttaagaacaa ctttatttat ccaggtcaaa
     4561 cgttgattgt agctaaataa aatagctgtt cgaagaaata acgattgaat tccacaaaaa
     4621 tatttgttac tataaggcta cttaaatatg acaacacttg aaaaggaagt agtaaaaaga
     4681 ctgaagtttc agagagcgca tggctggtga gaatgtgcac ggatgctttt gaactcgcct
     4741 ttgagttgcc cttgctgaat acccgagtaa gcaagcggcg gtgacggccg ttacaacgtt
     4801 cgaggccttt tcactaaggt aaaattaggt ggtaccgcgt agattacgtc ctataaaaag
     4861 agcaatcttt ttataggatt tttttatttc ttttgtgaag tttgagaaaa caaagcaaaa
     4921 ggaaataaaa ataaacagat tctgccggtt ttttcgttat atttataata gaaacaagtg
     4981 ttttcaatag aacaggagga cagcaaatga gttacaatca taaagaaatt gagaaaaaat
     5041 ggcaaaaata ctgggcaaaa acgaatactt tcaacaccca tgatgatcct gaaaagccaa
     5101 aattttacgc actggatatg tttccctatc catccggtca agggctacat gtagggcatc
     5161 ctgaaggtta tacggcaacg gatatccttt cacgtgtgaa aagaagtcaa ggatacaacg
     5221 tgctgcatcc aatggggtgg gatgcattcg gtctgcctgc tgaacaatat gcgctagata
     5281 caggaaacga tccagctgag tttacccaaa aaaatatcga aacattccga cgccaaatca
     5341 attctttagg atttagttat gactggaatc gcgaagtgaa tacaactgat ccggagtatt
     5401 ataaatggac acaatggatc tttacgaagc tttatgaaaa aggtctagct tatgaagcag
     5461 aagtagcggt aaactgggta cctgaattag gaacagttat ttcaaatgaa gaagtgatcg
     5521 atgggaaaag tgaacgtggt ggctatgatg tagtcagaaa accaatgcgc caatggatgc
     5581 tgaaaatcac tgcttatgct gatcgcttat tggatgatct agaattagtt gattggccag
     5641 aaagcattaa agaaatgcaa agaaattgga tcggtcgttc agtaggagca aatattgaat
     5701 ttaaagtggc tggtacggat aaatcctata ccgtatttac gacacgacca gatactctat
     5761 tcggtgcaac ttactcggtt cttgcacctg aactagatct tgttagagaa attacgaccc
     5821 ctgaacaaaa agctgctgta gaagcttata tcgaagagac agctaaaaaa tcagatctaa
     5881 agagaacgga tcttgcaaaa gaaaaaacag gagtatttac tggtgcttat gcaatcaatc
     5941 cagttaatgg caaggaaatc ccaatctgga tcgccgatta tgtattatct tcatacggca
     6001 caggagccat catggctgtt ccagcacatg atgaacgtga ctttgaattc gctcaaactt
     6061 tcggactaga aattcttcca gttatcgaag gtggagatgt tgaaaaagct gcttacacag
     6121 aagatggtac gcacatcaac tctgaatttc tagacggcat gaacaaacaa gaagcaatcg
     6181 ataaaatgaa tgaatggcta gaagaaaatg gcgtagggaa aaaagaagtc agctatcgct
     6241 tacgtgactg gctattctcc cgtcaacgtt actggggtga accaatccca atcatccatt
     6301 gggaggacgg aacagtcaca gctgtcccag aagaagagct accattacgg ttgcctaaga
     6361 caaaaaacat caagccaagc ggtactggag aatctccatt agcaaatatc gaggagtggg
     6421 tcaatgtcgt tgatcctgta acaggtaaaa aaggccgtcg cgaaacgaat acaatgccac
     6481 aatgggcagg aagctcatgg tattacctgc gttatatcga ccctcataac aaaaaagaac
     6541 tagcagatta tgaaaagttg aagcgttggc taccagttga catctacatt ggtggagcag
     6601 aacatgcggt acttcattta ctatatgcac gattctggca taaattctta tacgatatcg
     6661 gtgttgttcc tacaaaagaa ccattccaaa aactatacaa tcaaggaatg atcttaggcg
     6721 aaaacaacga aaaaatgtca aaatctcgtg gtaatgtagt caatcctgat gatgtggtcg
     6781 aaacttacgg tgcagataca ctgcgcatgt acgaaatgtt catggggcca ttagatgctt
     6841 cgattgcttg gagcgaaaat ggcttagaag gaagccgtaa attcttggat cgtgtatggc
     6901 gtttgatcgt tgatgagaac aataaaatgc gtgaccgtat cactacattg aatgatggca
     6961 agcttgataa agtgtatcat caaacagtga aaaaagtgac agaagactat gaaaatcttc
     7021 atttcaatac agcaatctcc caattgatgg tctttatcaa tgaagcctat aaagtggacg
     7081 ctctgcctta tgaatatatc gaaggatttg ttcaactact cgcaccgatt gctcctcaca
     7141 tgggtgaaga actatgggca atccttggaa atgatggtgg tatttcttac gcaccatggc
     7201 caacatacga tgaagcagca cttgttgaag acgaagtaga agttgttttc caagtaaacg
     7261 gtaaagttcg tgcgaaaagc aatgttcccc gagacttagg aaaagatgag ctagaaaaag
     7321 cagcattagc caatgaaata gtgcaagaat atattgaagg caaaacagtc agaaaagtta
     7381 ttgcggtacc aaacaagtta gtcaatatcg ttgctaatta attggctctt tgtcaataag
     7441 gactgatgag ttgtgcaaaa tcaaatctgg gccagaatga acttcattct gtctcagatt
     7501 ttttgttatt tcaaattgat taattgattg atcaaaaagg ttttgatttt catgttctca
     7561 aacacgttca atgtcttttt cttctgaatg gtcttgtaag agatctctgt aatcatcaaa
     7621 tttttgtcta ttaatcttag tattttttga tgtataaact acctgaaatt gagagtacat
     7681 accaaattca taaatttttt attagtggga ctagtgattg tatggatttt gacgcataat
     7741 gaatgaagta tgagaaaagg agagacaaat ggatcaagaa ctatttatta tcgaagtatt
     7801 atcagctaaa caaaaaatta tcgagaaagc gaaaaaaaga gcgaaaagaa taatgttcaa
     7861 gcttttaagc ttagtatttt tctctggttt gctttttttc ggagctgtat cgttcctata
     7921 tcgccatatg agtgcagtag tccgttcagc tgaagggatg caaaaattaa ataatttgta
     7981 tcacttttcg tggggagttg gcggaataac aattgttgtg cttattgttt ttcttctact
     8041 tatttggctc cgttaccatc aattgtcgcg tcatttcgat caatggctta gtcaaagagc
     8101 acaacagttt ttacataata aaccaacttc tcaagatgat gactatcttt attttgagaa
     8161 gacttcgacg agtcaagctt ttcggataca aaaaagtgat tgccagttat tgctgagatc
     8221 acttgatgat ttacctatct acttaggcga aagcaaatca aaattcggaa tgaacacatt
     8281 ccatattttt ttactagaaa acgaacccag atgggaaaaa agccagtctt cgcctggtgg
     8341 atacctgaca actaagatga gtacattagt cactggtttt ttcctcattc ttttagctgg
     8401 aacaatctat tatgggagat acactcagcc aaaaatgtct tctcatataa atgctgcttc
     8461 tcacatgaca atgaactcaa ctaacgatac gcatatacaa aagacaaatg aagggttgtt
     8521 agctgatcct aagcaaaagg taaatttgac aacacaaaag ataaatgatc tcaaacttga
     8581 tacagagacg cactctctct atatgacaac agatagtggt gctaattggg tatttgtgcc
     8641 gattgattcg caatggttgc gttttggcga atacaccctg acaacggggc tgattcctga
     8701 aggttattgg atggacaaca catatgatat ttccgccgat ttctcatggt ttatctattc
     8761 ttctggggat gatgtacgtc tgctgacttc aagagataat ggtaaaacat ggcaaacgtc
     8821 tattatcacc caatcagttc agccgatacg ttatcgaaaa gcggtatttt atggcaacga
     8881 tggtctattt atctgtacat cagccagtgg gatgtctgca gaaactttgt acatttacac
     8941 tactatggat ggcggaatca cttggataaa aagtggaagt acaatgatca atcagccagt
     9001 acaaaatgtc agcttcgtta caacttcttt aggttttgtt tcgacacgtg aaagtttgtt
     9061 ttatacaaac aacggaggac gctcattcaa agaatcagtt ctgtcgattc cggaaaacta
     9121 tcagacaggg gggatagatc tttttcaatc accaaatgaa gttactcaaa taagcgcgaa
     9181 tacgttagag acaaaatttt atttgttgaa aaaaggacca atcgatgcag ggaaaatgtt
     9241 tgcctgcctc ttccgttcaa ctgataacgg ggaaacatgg caattcgttc agcaactatc
     9301 gcaagtagaa caaggagatt aaagaagaga gattgtgagc ctgacgttca gcaccgagtc
     9361 acaagaaaca aattcaaaaa acagcttctc atatttgttg aatagtgtaa ctcgtgattc
     9421 ggagttgaca agagcaacaa gaagcgattt tgaagtatag cttctcgtat tttacaagat
     9481 cgtgtcttat tgccgatagg ttgtctcttg aacaccgttt attcgtaaaa agggattgtg
     9541 acaagtcttt tgtcactact taccgaacga aactgaagga gctatccaaa aagtatcatt
     9601 agtattattc tttgtgtttc tagtggcacc actagtacat ttaaaaaagc tatcgatcat
     9661 ggactaatca tacatgatcg atagcttttt agataatgtt tgttacgcta tctgaaattg
     9721 actattataa agactggcat aaagcgttgg tgattggagt agttcagaat gtgttccttt
     9781 ttcgacgata tcgccattct tcattacaag gatcatgtct gcattttcaa tcgttgacaa
     9841 acggtgtgca atcacaaaac ttgtcctatc ttttgtcaca gcttccattg cttgctgaat
     9901 caatgcttct gttcgtgtat ctacgctaga agttgcttca tctaaaataa cgacaggagg
     9961 attcgctaga atgactcgag caattgtcaa taattgttgc tgtccttgag ataacgtgcc
    10021 gttttcgcta gaaatgatcg tatcatatcc gtctggcagt gttcgaataa agtgatcaca
    10081 ttgtgcgatt tcagccgctt cgataatttg ttctcttgtg gcatccattt tcccgtaggc
    10141 gatattatca gctaccgttc cttcaaacag ccaggtattt tgtaaaacca tcccaaaaga
    10201 acgacgtagt tctccacgtg aaagcttggt aatatcaaga ccgtcgaatt cgatctttcc
    10261 tttattcagt tcataaaaac gcatcagcag attgactaaa gtcgtttttc ctgcaccagt
    10321 tggaccaacg atcgctaccg tttgttttgg ctttactgaa aaatcgactt gattcatcaa
    10381 tattttatct gatgtgtaac caaactgtac atttttgaaa gctatttttc ctttcggttg
    10441 ttctaaaagt cttgcatctg gaaggtctgg aatttcatct gtttcatcta atatctcaaa
    10501 tatcctatcg attgctgcca ttgcggattg gatcgagttg atcacataag aagcggtaga
    10561 aattggttca gaaatctgat tgatgtactg aagataagct tgtaagaagc cgatcgtcac
    10621 accacctgat agaacaagca tagcaccgaa taccgcactt atgatgaagg cgagttgatt
    10681 aatgaaacga atggccggat agatagcaaa gttcataaat tgagcacgtt taaaagcgtt
    10741 gtattgcttt ttatttactt cgtgtatagc agcttctgca tgagcttgct gattgaatgt
    10801 tttgaccact agattaccag ctaagatttc ttccattcga ttatttaaaa cacccagttc
    10861 atactgattc tgttcagaaa tctgtttgtt tttgttcgca atcttagttg taacaattga
    10921 actgccaacg atcagcaata agacgagcaa agtaagcttg ctttcgatat aaaacagcat
    10981 cagaccagca gcagctattg ttacgacaga agtaaaaaat tgattgatac ctgtcaataa
    11041 cacttgagaa agttgattga gaccggtagt tgtccgacta atgatgtcac ctacttgatg
    11101 attgtcaaaa aacgacatcg gtaatgtttt gaattttttt gtgatttctt ttcgcaccct
    11161 caatgtgatc ttttcgctta acgctgccat tgtccgttcc tgaataaatg aagtgatgct
    11221 gcttaagact gaacagccga tcagcaataa gacagggaac aacaatgctt gtttgatatc
    11281 actgaaagaa agctgtccaa tacctgtttt tcttatttct gataagagat catcgatccc
    11341 gattcccatg atcaagggca tggctacgac taataggttc ccgaataaac tgcatagaaa
    11401 caatccgtaa aaatatttat gttcaggttt gatcattttc caaaaacgta tcaaagcacg
    11461 cgtgttcaac gtttttttct tttccatcgt atcccttctt tctatattcc ttgtgatttt
    11521 gcaaactcct gataagtttg gcaattagac agtagttctt catgagtacc ttgtccaacg
    11581 atttttcctt catctaaaac aatgatgtta tctgcttgtt ggattgtact gaggcgttga
    11641 gcaacaatca cgagagtttt ctccgacatg tgtttatgaa gggctgctct taatgctgca
    11701 tctgttttat agtctaaagc ggagaaacta tcatcgaata catagatctc tgctggtttg
    11761 atcaacgctc gagcaataca tatccgctgt ttctggcccc ctgaataatt agaaccgcct
    11821 tgagcaacaa agctgtccaa tccgtcagga agggtgtcca ctagttcttc taattgagcg
    11881 atcttgatcg ctttagacaa ctcttctgtt gaggcatatt catttcccat ctgcaagttg
    11941 cttaaaattg tcccgctgaa taagaaagcc ttctgaggga cataactgat gtgggagcgg
    12001 atctgttctt gagtcatttg agtaagagga ataccaccaa gggttacttt tcctatcgta
    12061 gggtcactaa gtctcaagag aagtttcgcc aaactacttt ttccagatcc ggtcccgcca
    12121 acgatcgctg ttgtttttcc tttaggtatt gtgaaatcaa tatcttctag aactggatca
    12181 gagatttcag tatatttaaa tgtgacatgt tcaaaagcag ctgcatttgt ctgatcaaag
    12241 gttggttcag ctactgtttt gatcgtctct tcctcaattt cgatctctgc ttgcagtatt
    12301 tcttctatcc gtcgtaatga ggcaaacgat ttagggataa tgaccaatac catagctgcg
    12361 atcatcaagt aagcaagtgt cagcatagag tattcgacta cagcagtcac gacaccgatt
    12421 tgtaatgctc cttctgctac aagatttccg ccaaaccaca aaatagaagc ataaacgatt
    12481 cccatgagca aaaaagcaat tggagtgacc caagcaaata ttcggttcac tttgatcatt
    12541 tgattagcat agtcagcaaa gctcttattc gttcgctttt cttcatatga ttgattatca
    12601 aaggcacgga tcatattaat cccagtaaag aattctctca atgtgatggt gatccgatcc
    12661 atttttggct ggatactcaa agataaaggt gcccctcgtt tcatcagtcg ataaacaatc
    12721 catacataaa aaacaacaga gatcaatgta acccatgtta gtaatagaga ataacgaaac
    12781 atcatgagaa tggtgaaaac gcaaataacc ggtgctggta agatcatttg gcaaaataaa
    12841 acaatcattt gctggacatt atcgacatca ttcgtcattc ttgtcaaaag agatccagaa
    12901 ctaaaacgac tgatatcctt cattgacagc tgttggaatt tacggaaata tttctcccgc
    12961 gtttggaatc caaacctagc ggaagccaat gctgaaaagt aactagaggc aactgcagcg
    13021 attgttccta gaaacgctac aagcagcatt tcaactccaa ttttttttat aactgaggct
    13081 tcgccacttg ctatacctgt gtcgatcagc tttgctacta attttggtac acctaatgta
    13141 ccggcgattt gtaaacagat catcacaaag gtaaggagag cgagtccttt gtttttctgt
    13201 aagaatgttt ttaagataga catattattc ctcctttttt cctgatactt ttcgattcta
    13261 taccttcaag taacttgaag gtcaatagaa aaaagaataa actgaaaaag aggtgagaaa
    13321 aaatgaaaaa aatgtatacg attggccaag tatcggaact atttgatttg ccaaaatcaa
    13381 cgatccgcta ttgggatgaa caaggattaa tccgttcaag tagacaagaa gagaatgatt
    13441 atcgattgtt tgatatcgat gatattttta tgctttatga tatcgttttt tatagaaaat
    13501 tagatatacc gatcaaacag atgaagaatt tatatggaaa aacattgacg gagttatatg
    13561 agacacttga tgaaacagaa agaagaatcc ataaagaact ggtggtgatg aaacaaaaac
    13621 aaaaagagat aagggagcga aaaaaacagt tgaaattgat gatcgataca aacgaggaag
    13681 aatttcctgt agaagagatc ccttttgact gtatgatctc aacggagttt gaagatattg
    13741 tggaaatcaa aaaatttcta cctaactatt caagctttgg gatgatgagt atgccagctt
    13801 ccagttcaca gacgatgtat ggatttttta tcgaccctag tgaagtacat ttatttactc
    13861 aagacgtgat ttgggagaaa aaagatacgg ctgtttatcg tcgattctta ttgaaatccg
    13921 agatgaacca tgcggaacgt aataatattt tagaaatcag agaaagaatg tatgaaaaag
    13981 gatggaaaac aggagaagtg atcggccagt atctattgac gaatacggat gaaaacaata
    14041 tcagaactga atattatcat gcatggatag aaatgaaaaa ataggaaata tgtgtttaag
    14101 ataactcgaa aatgatggtt gttttagtta taaagaaaaa ttttctggtt cgattacggg
    14161 tttaatgtgg gggattatta gtcgtggata gatagaattt tagtttttta gaggaaatat
    14221 gtctttaaaa atggaatggt tgcaattctt agcgatatat acgatgtaat tgtcctctta
    14281 aaaaagtatg gaaaaataat tgtattgggt gttatgtata attcatggtg aactgagact
    14341 aatgagaaga aaaattcata ttgttatgag tgagattgta tcagatgaga atgataaatt
    14401 aaataggtgc ataaaagcat tacacttaaa atacattgtt gcttacatta ttacaatatg
    14461 ttaataaggc aaaaataaat agatggattg aataaacata ttttttaatc aaatagtccc
    14521 tactgtgact ttcctcagct atcggtgat
//
LOCUS       DAXLPI010000064        14119 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXLPI010000064 DAXLPI010000000
VERSION     DAXLPI010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.064"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(31..3684)
                     /gene="rpoC"
                     /locus_tag="ACF3T5_001707"
     CDS             complement(31..3684)
                     /gene="rpoC"
                     /locus_tag="ACF3T5_001707"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFD3632310.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3763..7380)
                     /gene="rpoB"
                     /locus_tag="ACF3T5_001708"
     CDS             complement(3763..7380)
                     /gene="rpoB"
                     /locus_tag="ACF3T5_001708"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFD3632311.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8108..9076
                     /locus_tag="ACF3T5_001709"
     CDS             8108..9076
                     /locus_tag="ACF3T5_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3632312.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9094..9999
                     /locus_tag="ACF3T5_001710"
     CDS             9094..9999
                     /locus_tag="ACF3T5_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632313.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9996..10802
                     /locus_tag="ACF3T5_001711"
     CDS             9996..10802
                     /locus_tag="ACF3T5_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632314.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10959..11927
                     /locus_tag="ACF3T5_001712"
     CDS             10959..11927
                     /locus_tag="ACF3T5_001712"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFD3632315.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12244..12768)
                     /locus_tag="ACF3T5_001713"
     CDS             complement(12244..12768)
                     /locus_tag="ACF3T5_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFD3632316.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13055..>14119
                     /gene="scm"
                     /locus_tag="ACF3T5_001714"
     CDS             13055..>14119
                     /gene="scm"
                     /locus_tag="ACF3T5_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFD3632317.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         3997 a         3047 c         2784 g         4291 t
ORIGIN      
        1 taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca ttgcgtcttc
       61 tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg ctacaccgac
      121 ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga tgatcttacc
      181 tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga tcgcagcatc
      241 cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat ttgtttctaa
      301 tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc ctcctgagac
      361 tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac ctggtaagat
      421 atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa ccattacttc
      481 gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta cttcacgaag
      541 caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg gatcgattga
      601 tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt tcatacgtgc
      661 tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta cttctttggt
      721 acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg tgatcactgc
      781 ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac cttgagtgat
      841 atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag taccaggttc
      901 accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt cagagccagt
      961 tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac atgtgaagac
     1021 agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga tgtcttcgga
     1081 aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta cttctttacg
     1141 ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc cctcgcggat
     1201 ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga ttacatcttg
     1261 ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca aggctgtatc
     1321 ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg atagtccttc
     1381 acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg ccatcaaacc
     1441 acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag aatcagacat
     1501 catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga tgctgtcttt
     1561 tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga tcagaccacg
     1621 acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga tttcttgttt
     1681 ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg tcgaatgttt
     1741 gtaccctaag tctttcatac ggtcaagcat cttagaagtt tcttttactt tgaatcgttt
     1801 gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata ctaattcttg
     1861 ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt ccggtgtttg
     1921 gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag gcatgatctc
     1981 gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc attcagtaaa
     2041 tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat gaacatatcc
     2101 gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt cgccttcgcg
     2161 tccttcttct tccattgtca agtagtagtt acctagaacc atatcttgag aaggagtaac
     2221 aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca acatacgtgc
     2281 ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc catcgaaatc
     2341 ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt gtactaaaac
     2401 tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca gcaatactgg
     2461 gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac gttcgatttt
     2521 acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac gcatcacgaa
     2581 tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca tcttcaagaa
     2641 aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta gcaagttttg
     2701 acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg ggcggttacc
     2761 tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta ctgcttcttg
     2821 cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta acaaacgttt
     2881 caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac ttgttgcaaa
     2941 acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag ggataacgtc
     3001 cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa tatccaaacg
     3061 gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt tcaattcggc
     3121 tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg cacccatagc
     3181 agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt ctgtcaacaa
     3241 ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag caaaataaat
     3301 gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg aaggaatccc
     3361 tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca tgcgttcacg
     3421 gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc ctttataacg
     3481 gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa aaatccgctc
     3541 acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct caggtttttt
     3601 tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta tttgcatact
     3661 ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat ggcaaaccgc
     3721 aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta aattatcttc
     3781 tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt cagcagcttt
     3841 cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat caacagtgat
     3901 caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt cgatatccaa
     3961 gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga atgattccgg
     4021 tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt tcacacgtcc
     4081 gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg caccgtaagc
     4141 ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag ctttacctcc
     4201 caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca atttgtcatc
     4261 gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc catcaaatgg
     4321 ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac cggcttcacg
     4381 aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg ctacgtggat
     4441 acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga tgttcatacg
     4501 tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag gtaggaatgg
     4561 catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac gaccggccat
     4621 tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta acatgtttac
     4681 gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt gaacgatacc
     4741 gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt tttcgccaaa
     4801 gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg gcgtgacttt
     4861 ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac ccatttcgtc
     4921 taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt cttcaggtcc
     4981 taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag tgtatacgtc
     5041 atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc cttcccaagt
     5101 catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca tagatggtcc
     5161 gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg gacgttggtt
     5221 gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat ctaatgcacc
     5281 gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac catcatgttt
     5341 acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag taccaaccca
     5401 tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac ccatcaaggc
     5461 acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg caactacttg
     5521 tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt tttcactttg
     5581 agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg ggctgtttgc
     5641 ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt ggtcagtaac
     5701 acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga atttatttac
     5761 tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag gagtttcgat
     5821 cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac cggcgcggtc
     5881 acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt ttgtgtgtca attcgccaag
     5941 agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt ctttgatact
     6001 tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag tatcttggat
     6061 cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga attggttttg
     6121 taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat catcgacatt
     6181 gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt cggctggacg
     6241 aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt cacgttcagg
     6301 gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga ccacaccgtc
     6361 ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag ctagtgtttc
     6421 catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg tttctggatc
     6481 aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta attttttgtt
     6541 tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga aacgagcatt
     6601 cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac gttcgtaaac
     6661 gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt ctttttcgac
     6721 tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat cggaaccaaa
     6781 tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga tacgtacata
     6841 agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag ggataacagt
     6901 agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga cacctggtga
     6961 acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg ttccttgttc
     7021 tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc cagtctcgcg
     7081 gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat gcgcacgagc
     7141 ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca aagatagatt
     7201 gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc cctcatctaa
     7261 gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca acacttcact
     7321 gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt gtccagccaa
     7381 gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt tacaataata
     7441 tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg caaaaaaaaa
     7501 ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt gtttccttat
     7561 attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat agtttttgca
     7621 acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt tcttctgctt
     7681 tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga caattcttaa
     7741 ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga ggaacttcta
     7801 tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac tttagctact
     7861 ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt tgtatctatg
     7921 atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct aagtggctga
     7981 gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta ttttccgact
     8041 tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa ttaaggagga
     8101 aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac tagctttagg
     8161 aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa caattggcgt
     8221 attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca aagaaggatt
     8281 agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa atgcccaaaa
     8341 caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc ctgatttgct
     8401 acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca ctatcccgat
     8461 gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca atgaaaaacc
     8521 tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa tcggcttact
     8581 gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg gtgaagcaaa
     8641 ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg tcaaagtcaa
     8701 agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc tagctaaaga
     8761 tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa gtgttgtcgg
     8821 tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc aagtcaaaga
     8881 cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggcaaacaaa cagggatcat
     8941 ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa cagcgaatga
     9001 tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc cagacagcat
     9061 caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc tcttttctta
     9121 tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata cgtcactttc
     9181 cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg tgcagggatc
     9241 gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct aggcttttta
     9301 gggagtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa aattcccgcc
     9361 cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg tgtgatgggt
     9421 gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga gtcattcggc
     9481 ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat cgttgtatgt
     9541 ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac tggcgataat
     9601 ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat cggttatatg
     9661 atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa cggctttgct
     9721 gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat catcgcagaa
     9781 gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt aggtgcggtc
     9841 atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag tgattcgaaa
     9901 cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca gcaaaaattc
     9961 catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta acattggaaa
    10021 atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg aaaggcatta
    10081 atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg gctggtaaat
    10141 ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa attttattga
    10201 atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc agtcgcgtat
    10261 tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat cttgctcttg
    10321 ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa gaccgaacat
    10381 ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta tcctcagaaa
    10441 tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc acattacagc
    10501 gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct acttccaaaa
    10561 cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct tttatggtca
    10621 ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg catcaaggaa
    10681 ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc caattattag
    10741 cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt ttatcttcat
    10801 aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt ctctttcctt
    10861 ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt gttctctcct
    10921 tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa gacaaggtac
    10981 ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca cagcaactgg
    11041 aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca ggctatcgtt
    11101 ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat gcaaaagaaa
    11161 tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg ttgacttctg
    11221 aatctacgat gaaagatgcc aaactggcag cagctcaagg agattctgtt tcgaagttat
    11281 ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt ttcgctgaac
    11341 ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt gatgaattgc
    11401 ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct gtaaccggtc
    11461 gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa gtctgtggga
    11521 ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc atcattggga
    11581 tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa gaaaaagcca
    11641 tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc agcaaaatct
    11701 ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc tataaagaaa
    11761 aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact tatgtcggtc
    11821 ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa cagaagatga
    11881 gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta tcagatttca
    11941 caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca aaagactctc
    12001 cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta aaaatatcta
    12061 taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag tcatcagttt
    12121 acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata gaccatgggt
    12181 aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt tttagctagt
    12241 gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag agtgcatagc
    12301 agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg tccagttctc
    12361 gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa tagcaaaatc
    12421 agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga tctgctttgc
    12481 ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg gaaaccggct
    12541 ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa tctggaaatc
    12601 taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg gggctctatc
    12661 ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac gttcaaaccg
    12721 atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt attttcctcc
    12781 tttttattta tcctacttac tttttcctaa ttctaggata cctacttata tgttactaca
    12841 cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga ttgaaagaaa
    12901 aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt cattaatatt
    12961 ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa aagagtataa
    13021 taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa gtttactagt
    13081 cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag cggaaacata
    13141 cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg gcttgttagg
    13201 aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg cacatacaga
    13261 cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag agcttcatga
    13321 aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg ctaattcttc
    13381 aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta acgtggatgt
    13441 agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact taactccaga
    13501 tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg aatttgctaa
    13561 attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat atactgctgc
    13621 agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag ataccgggtt
    13681 tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc aaatcattaa
    13741 tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat tgaacgtaca
    13801 gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt cggatgcgaa
    13861 tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc cttttcaagg
    13921 aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg gaacgattat
    13981 aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg aaatatttat
    14041 cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat ctaccacaag
    14101 tgatagctca gattcatca
//
LOCUS       DAXLPI010000065        14000 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXLPI010000065 DAXLPI010000000
VERSION     DAXLPI010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.065"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(26..319)
                     /locus_tag="ACF3T5_001715"
     CDS             complement(26..319)
                     /locus_tag="ACF3T5_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFD3632318.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(347..1012)
                     /locus_tag="ACF3T5_001716"
     CDS             complement(347..1012)
                     /locus_tag="ACF3T5_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFD3632319.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1152..1784)
                     /locus_tag="ACF3T5_001717"
     CDS             complement(1152..1784)
                     /locus_tag="ACF3T5_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD3632320.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1791..2858)
                     /gene="liaS"
                     /locus_tag="ACF3T5_001718"
     CDS             complement(1791..2858)
                     /gene="liaS"
                     /locus_tag="ACF3T5_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFD3632321.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2855..3586)
                     /gene="liaF"
                     /locus_tag="ACF3T5_001719"
     CDS             complement(2855..3586)
                     /gene="liaF"
                     /locus_tag="ACF3T5_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFD3632322.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(3754..4233)
                     /gene="greA"
                     /locus_tag="ACF3T5_001720"
     CDS             complement(3754..4233)
                     /gene="greA"
                     /locus_tag="ACF3T5_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFD3632323.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="ACF3T5_001721"
     CDS             complement(4369..5544)
                     /gene="mltG"
                     /locus_tag="ACF3T5_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFD3632324.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            5709..6869
                     /locus_tag="ACF3T5_001722"
     CDS             5709..6869
                     /locus_tag="ACF3T5_001722"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFD3632325.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            complement(6909..7856)
                     /locus_tag="ACF3T5_001723"
     CDS             complement(6909..7856)
                     /locus_tag="ACF3T5_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFD3632326.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(7989..9380)
                     /locus_tag="ACF3T5_001724"
     CDS             complement(7989..9380)
                     /locus_tag="ACF3T5_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFD3632327.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            9506..9916
                     /gene="tagD"
                     /locus_tag="ACF3T5_001725"
     CDS             9506..9916
                     /gene="tagD"
                     /locus_tag="ACF3T5_001725"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HFD3632328.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            10330..11130
                     /locus_tag="ACF3T5_001726"
     CDS             10330..11130
                     /locus_tag="ACF3T5_001726"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFD3632329.1"
                     /translation="MRLRKQHYHYQSCGKYFTAHTYLVVPSCFISKQVHYTILEELTE
                     RQALKTLTKRCDLSVTTFQRTLSALSAMLRQMLDYLPHCLLFDEFRSLSISHGKFSFS
                     CMNRETGKLFDILSSRRKKDLIAYFMRFTRKAHMGVRYIVTNMNAPYFSLVKESFPNA
                     QIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKDTSQAKYYRQLKPLYKLLLKSDDELDY
                     TTFKKWQNFQWRYLTESEVVDRLPSISYELKIAKQFIKSCLAIITRNS"
     gene            11239..11385
                     /locus_tag="ACF3T5_001727"
     CDS             11239..11385
                     /locus_tag="ACF3T5_001727"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3632330.1"
                     /translation="MTKLENLRTHIKTLKRFAYGFRSFTNMRLWIFLINRLISYALKA
                     SNSY"
     gene            complement(11452..12879)
                     /locus_tag="ACF3T5_001728"
     CDS             complement(11452..12879)
                     /locus_tag="ACF3T5_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFD3632331.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(12876..>14000)
                     /locus_tag="ACF3T5_001729"
     CDS             complement(12876..>14000)
                     /locus_tag="ACF3T5_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HFD3632332.1"
                     /translation="FFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVISEKIHE
                     KKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTIKYAKRK
                     KQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKKYVSAFN
                     IVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQLEWLGVD
                     NLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLEMLFEEI
                     DVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDSYGLVDV
                     FKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
BASE COUNT         4580 a         2680 c         2075 g         4665 t
ORIGIN      
        1 aaaacttgtg tcaccctctc tttttttatt gttttttaaa gtgataggtt ggctcctcca
       61 ggttgggatc ataatcaaca agtaaatcat atcctttaaa gaaccattca tctgcttcct
      121 cgataaaaaa aagtgtccca tccatttcca ctttctttac aggatgttct ggctgttcaa
      181 ctgacattcc aatcgaaaaa ccatcatgaa cctctgtttt gccgtagact tttccaaaaa
      241 aacgtattcc gtaagtttct ggcagatcaa tttctgagtc aaaccacttt tgtgcttttg
      301 gcgttaccgt taattccatc ccaacaacct actttctctt ttctttctat tgattcatct
      361 tatcgtctag aatatccaca tcgcctgtct ctccaatcac cagtagatta tcattttcgc
      421 gaacatattc atctgctgca ggagaagcga taaccgtttg atctactctt cgtatggcga
      481 ctactgttaa gtcaaaacgt tgcctgaaat ttaattcagc taatgttttg ttgaaaaatt
      541 taggattgga gactcggatt tctgctaaag aatatttatc tgacagctct aagtagtcta
      601 gaatattctt tgatactaag ttatgtgcaa tccgtatccc catgtctcgc tcagggtgta
      661 cgacacgatc tgctccgatc ttctctagta cacgggcatg gtactcattt tgggcttttg
      721 ctaaaatatt cggtacgccc atttccttta ccatcagagt aactaagata ctggcttgga
      781 tatcttctcc aatagcgaca attacatgat cgaaatttct tatacctaat gaacgaagtg
      841 tcatctcatc ctgtgcattt gccacaacag catgagtggc tatattcata tattcattga
      901 ctcgatcttc attactgtca atagccagta cttcttgtcc agcttcgatc agtgtacggc
      961 agatgcttcc gccaaatcgt ccaaggccta caattgcaaa attttgtttc accattttcc
     1021 tcctttagta tcttcttttc tcagtataac aaagcctttc caaaaacaaa agcaagcatc
     1081 attattgtaa aaaagccaag aaaaataata gttattttct cagcttttga ttatcctctt
     1141 tatcatcgtt tttatttcac caaaccatgt ttaaacgcat agatagctgc ttgagttcgg
     1201 tcttctacct ctaacttagc taatatattc gacacatgcg ttttaactgt tttcagtgtg
     1261 ataaacaatt catccgcgat ttcctgatta cttttccctt cagaaatcag catcaaaatt
     1321 tcattttctc gattcgttaa ttcttcatgc aatactggat catttcgccg actcattttt
     1381 tccatcattt ttgttgtcac ttcaggttcc aatactcgct cgccgcgttg cgtagccctt
     1441 atagcatcag ctatttcatg tgcggtagac gtcttcaaca aatatcctgc tgctcctgct
     1501 tcaattgcag gataaacttt ttcatcatca ataaaacttg tgacgataat gattttggct
     1561 tttggacaat cttttaagat tgcttttgtt gattcgatgc catccatttc ttccatgact
     1621 agatccatta aaatgacatc tggtcgaagt gccatcgcct tttcatagcc ttgacgtcca
     1681 ttttcagctt ctccaatcac ttcaatgtct tcttggatag ataaataaga agagacgcct
     1741 aagcgtacca tttcatggtc atctactaat aaaactttta tcatctttat ctatgcctcc
     1801 ttcatcaaag ggaccttgat ctccacgctc gtaccttgcc ctttaaaact aatgattttt
     1861 actgttccac caataccagc tactcgttct ttgatattgt tcaatccata acttcccgtt
     1921 ttggtttcat tcatattaaa tcctgtcccg tcgtcaatga tacgtaataa aagattgtta
     1981 tctattttat gcaaatagac ttctaattca ttagccttag catgtcttaa tgtgtttgat
     2041 aacaattctt gaacgattcg gaacaagtga tcctcaatgg aactagttaa ttttacatct
     2101 tctacatccc attttagcga aatctggatt ttattctgca attccttcaa taactgctca
     2161 atgccttgtt ttaaactttt ttcttctaaa ttgactggac gcagatgaag aagcagcgca
     2221 cgcatctcag actgggaagc gttgatgatc tctgccacca ttttcaactg cttacggaac
     2281 atttctggtg tctcactttt ttctgcttgc tccgtcaatg ccgacatcat catcatcgct
     2341 gcaaataatt gctgtgaaac tgaatcatga agctcacgag ctaatcgatg cctttctaat
     2401 tctaaaatct cttctttggt ttgtccatct acatattgag gacggctagt cataatctgt
     2461 aattcactgg atacttcgat cattttttct tttattttag caatttccat atcaatatct
     2521 ttgatgtaca gatcatccga cgcgcttggg atcggctgat gcaaaagttt ggattcataa
     2581 ttgccagaag aaagtgcacg aagcttttct tcgattttcc cgtattttgc cttttgaata
     2641 agtgataaca atagaaatac gatcaatcct acacctaatg aaattgccat caaatggaag
     2701 ataagcggaa cgtataataa acgcgcacgc agcaattctc cccaccaatg actttggtta
     2761 ctggcataaa aataagtgaa aagtgaaaat agaataataa ggaaagccgc aatccccgaa
     2821 taaacagcta gcattgcttt ggatattctt cccatcatat tcgtatcacc tcgacatctc
     2881 caaccaaggt attggtaatg atcttcaatc tgcgaggatt ttcatcataa tcactgctgc
     2941 agattttgat ctgttcgttt tttaaggaaa actgctcttc ttcaaacaag acatttccta
     3001 ctaatgtcgc atgctctaaa cgaatagcca ctcccagggg tactaagatc cgtgttctgc
     3061 ctatcccttt tcttacaatc acaatattat catcttttgg caaaagtgta ttacccaaat
     3121 cgatgatagt atcaccagaa atgatggcaa tgttgatatc atcccattca tagacgtcgt
     3181 ttccaatacg ttggttacca aataattgct gtcttttacg ctcattatta tgtgttttta
     3241 attgctctgt ttggaccatc atgatctgtt tttttcgcca aaaagcattt tttgtcaaat
     3301 ctattcctga aatctctaca cctttcagcc cgataaataa aacggccaat acaaccatca
     3361 tccataaagc aggactgttg actaagctaa aaaaaatgac caagctccct aagatcaatt
     3421 gaaaattttg aaatttagta cgaggatatt tgcgcatagc tagatatata ttgaaaatac
     3481 cgaatattac taaaagcaaa agtccagtat tgtttacgat ttgccaaaca gcaaataaca
     3541 gcagcagtgc ttcgactacc acaaaaaaac gccaagaact attcatcgct ttttctccct
     3601 cctgacacat tctcatttca tcatacctat ttttttgact gttggctata ggactttaga
     3661 tgtaagatcg ttcagtgaaa cctgcaatac tcaaagagaa aagatctaga gtgcgtcatc
     3721 agtaagatga actctctaga tctttcagtc agactacgct tgttctactt tgacgatttt
     3781 aactaacata tcgccacctg gtgtagcaat ggttacttca tcatttttac gtttgccaat
     3841 caatgcttgc gcgataggag aatcattgga aatctttcca gaaaacgggt ctgcttcagc
     3901 acttccgaca atcgtgtact cttcttcatc tccatcaggc aactcgatga acgtaactgt
     3961 tttaccgatc gatacctcat ctgcatctac gccgtcatta tcgatgattt gtgcaaaacg
     4021 gatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat ctttagctga
     4081 ttcgtattcg gagttttcgg aaagatctcc gaaaccgcga gcgattttga ttcgttcaat
     4141 gatttctccg cgtttaactg tttttagttc ttctaattct ttttctaatt tttctttacc
     4201 ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt ttcgataaaa
     4261 taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac gcttttctta
     4321 aaatgtaaat gtttttctaa aaatgatcaa tgactagccc ctgtttttct aagaattatt
     4381 tacatatttt tgcactagtt ccatatgttc ttcatacgtt ttagcgaaat aaacatttcc
     4441 agtgctcgta tctgctacga aataataata gtcattttct gctggttcta atactgcctt
     4501 gattgccttt tcgcttggat tgtcaaaagg accaggccca tagccggtat tggtatacaa
     4561 attgtatggt gaatctacag ctgtatcttc atacgtcacc aattctttat gttctcctaa
     4621 tgcatataaa acagagatat ctgactgaag tggcatattt gccttgattc ggttaaagaa
     4681 gacttgtgca atatttttac gatcattttc ttttacgcct tctttttcaa ctagagaagc
     4741 caaagttaaa acttcttgta cagtcaaatt tttctgtttg atagctgaat aatagttttg
     4801 catgaccgta tttgtcttgt tcaccatctc aatcacaaga tccttaagcg aagtactcgt
     4861 gtagtaatca taagtagctg ggaataagta gccttccaaa cggtattttg tgttctcagc
     4921 tttagaggca ctttccaaca attctgggaa tttttgatac aactcgttaa agaaagtctc
     4981 atcatttact aaatctaaaa attctttctt gtctttacct gttaccttcg ctacccgctc
     5041 agcaatctga tcgatgtcgt agccttctgg aatggcaatt ttcccatctg caacttttgt
     5101 tggctcagat gtgccgcctt cttgcaactg cttgccaatc tcatcaagag tcatattagg
     5161 cgctaactga taataaccag cttgaaagcc agttaagttt ttaaattttg tataataatt
     5221 gaaaacaatc ccacttttaa taatattgtc tttctccaaa atttcgccaa tctgcttatt
     5281 agaagagccg tttgggattt ccacttggac taattggtcg tccgatttat ctaaaggctt
     5341 caacccgcta tctacataac gatatacggt aaacccgaga aatccgccaa taatcaataa
     5401 tgtaagagcg atcactaaaa tgatcttcct cacgatctta tcttcttttt tacgcagatt
     5461 tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt tgtctttgct
     5521 tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcatcttc gtaactctgt
     5581 ttattataca tgaaatttct actgccgtga attattttac caaatattat gtcaaaattg
     5641 taagtggcaa atgctctttt tgaggattac aatgaaaaat agttaaaaat ggatagaaag
     5701 gatttaatat ggcaaaagga aaacatcaaa aaaaacagca gatctctctt tcttttctcg
     5761 cagttttgtt gctggctgtc agcttctttt caacaggcat cattacagga tggcaattaa
     5821 caaaacaacc tcaaccacaa atttataaaa cggaacccgc acaacttgct tccaatttaa
     5881 catctaatga gcagatgctt gccagcaaaa ttgatcaatt actgcaaaaa tcagattata
     5941 tcggttcgat ttatgtacgc aaaaataatc gagtgatatt agaaaaagga tatgggtacg
     6001 caaatctacg cataaaagaa tcgaattctc cttctcttta ctatcaaatc ggttcgatcc
     6061 aaaaagcgat gactgcttta cttatcctaa aacaaattca atctggcaag ataacttttg
     6121 acacaaagct atctgaattt tatcctcaga ttcctggaag tcagcaaatc tcaatcaaaa
     6181 atatgcttta catgcgctct ggtcttcatc gaaccgcttc tccaaaaaaa cctatgtcag
     6241 atagtgagat cattcagttt gcccttcatc atttgaaatt tactggttat gatacgtatc
     6301 attatgaacc attaaattat acattgctta caggggtatt gatcaaacta atgaatcagc
     6361 cttatgaaac tcttttaaaa aaagaaatca ttcggccact ccatctagaa catacagatt
     6421 tttatgaaaa tgtaaaaaat tcccctcagc atgctgtatc ttaccaaatg tctgcaactg
     6481 acgattattc aaaagcattg gtcgagcctg aaacagacat ccgaaatgaa ttaggcacag
     6541 gaaatataag tatgtctgtt tatgacttga acaaattctt taccagtgtt ctgtctggcg
     6601 atattatacc aaaagaacta cttttctcat tgtggcaaaa taatggtgat aaacatcctt
     6661 acagcggcgg tgtttacagt ggaaatgatt atatccttgc tcagggaaat atcaatcgat
     6721 tccatgcagt agcggcgatg aaaaaagaca caaaagatgc aatcgtcatg gaaagtaata
     6781 tccaatctga taaaaacatc aaattgccgg ccactgattt aagaaatcag atttacgaac
     6841 tgatggaagg tgttaattta aaaagctaag tcgagacaaa aaaaccagca ggatgctggt
     6901 tttttgtttc aatcgatttc tgtctttaaa taacgttcca ttccgttcaa tatatcaaat
     6961 ttgggttgat aacctaaaga acggatcttt gaaatatccg ctaaagaatc acgaatatcc
     7021 cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg atcaattgaa
     7081 tgaatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg ctgtcctaaa
     7141 gctttttctt ccttagcaac aagtaaaagt gcttgtataa catcttctat ataaacgaag
     7201 tctctagatt ggctaccatc accaaataaa gtaaattctg ttttttctcc tgccaattgt
     7261 tttttgtatc ggtccactag aatcgaaata actccgctat atggagagtt aggattttga
     7321 tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata taaatgacag
     7381 taatccaaaa catattgttc tgatgcaaac ttatcgatgg catatggtgt taaaggacgg
     7441 atgaccgatt cttctttttt tggcaacgtt ggttcatcgc cataaactgc agctgatgaa
     7501 ctaaatacca atcgttttaa accagattga tattttcgta ccaattcaag caataaaagc
     7561 gcactgtcaa aatttacacg atgagtttct agaggacgtt ctacagaatc tgccacacta
     7621 gctacagcag ctaaatgaaa aatataggca aactgatact gtttcatgat tctttccatc
     7681 aattgaggat ctgatacatc cccctcaatg aaagtcactt gtttctccat atccaagttt
     7741 tctttttttc ccattgataa atcgtcgact actacgattt tgttttcttt tcccaaataa
     7801 ttcgctagcg ttgaaccgat aaaaccagct ccaccagtaa tcaatatagt atccatcaaa
     7861 ttcactccaa ttatgcattc tcatacttaa taaagttagt ttaccatttt tatattgtaa
     7921 aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt atatacctaa
     7981 aaatagtttc aattagttac tccttcttct ttagatatag ttttttggat agaacccaga
     8041 tatccccaaa aaataaatga acctatcgta ctaaccaaaa tgagttctgt gataaataat
     8101 gccgaaactg ctattgtaag aatcattaaa atagcaaaaa agtgcttatc ttttactaga
     8161 tttggttgtg tgaaacaata acgcaaagtt ttaaagcatt ggataaataa gaaggttaac
     8221 aatgtcaata gacctaatcc tcctgtagca gccataacta agaaaataaa attatgagca
     8281 gttaaagtat cttttcccat taatgtatat ggtagtttat catgtgcgta ttcaatatag
     8341 tatcctggag aaccgccgga tatcggctta ctcttgaata tttcaattgc acttttccaa
     8401 agtccaaacc ttaaattaga aacatccccg ccatcagtgt cagggcgttt taaatcaact
     8461 ttattgctgc tagttgttcc tgatgaatca gatatatctt tttttatttc tgtaatatgc
     8521 ggaacatgtg cccatgcttg agcagttata tctgttacat aatacatcgc aattatactt
     8581 agcaatccta ctactaatgc agttagccat ctttttaaag tattagtttt aagaaaacga
     8641 ctactataac ttgtaaagaa actaaaaaca aaagttgcta ttaataaaca aactaatgtg
     8701 gtgcgtgagc ccgataaagc aataaacgag aattgaataa aaatattaat tgcaaaaaat
     8761 attttaaata gaagatttaa atgcttgtaa gtgaccaaat aatatatagc aataatgatg
     8821 acaattaatg atataaccgc accaaaattt ggatctgtat atacaccaaa taaacggttg
     8881 cctaaccacc ctaaacgaac tgggtaatac ttatatttta ttggaatttt tatgccatat
     8941 tgaatgacaa accagtataa tgatactaaa ttagctaaaa accataccaa tgaaagtatc
     9001 caagtaaata ctttgaaaaa aaagttacta ctgtagtctt tgccaaactg ataaatcata
     9061 aagaaaaaga ttaattgcca catgattaac ttgatatttt cgctatatcc atattttaaa
     9121 ttcattaaac tgcttactag catcgcgcca ataaaagcaa ttaatagcca atcatatttt
     9181 ttaagatttt tttctctaaa tgcaataatt gcatctataa tgatcaagac aactcccgct
     9241 attccagcag gccaaaaaag taaagtatca tatctagtag gtaatagtaa atacgggcct
     9301 attgctcgta acattagaat aaaaccaaaa taaataaaat accaaccttt cacatatttt
     9361 aaaaattttt cattcaacat ttttcctcca tccacctatt tctcataaac atttcttatc
     9421 ctttttagga taaactggtt ctttcaattc taacaaaaaa atgctaaact aacaatgtat
     9481 attaatgaat ttcgagaggt gaaagatgaa aagagtaatt acttacggta cctttgattt
     9541 gcttcactac gggcatatca atttactgaa acgagcaaaa caatatggag attatcttat
     9601 tgttgccctt tctacagatg aattcaattg gaatgcgaaa caaaaaaaat gttattttag
     9661 ttatgaaaaa agaaaacagt tacttgaagc tattcgctac gttgatttag ttattcctga
     9721 agaaaattgg gaacaaaaag taaaagatat aaaagaatac catgcagatt atttcgtgat
     9781 gggcgatgat tgggctggac aatttgatga acttgaacaa ctaacagatg cacaagtaat
     9841 ttatttacca agaacacctg agatctctac aactcaaata aagaacgaac ttaattcttt
     9901 aaatagtgat aaataatatt ttttaaacaa tgaaagaaca atatatctgg ataagacgta
     9961 aaaaaatggc tctataattt ctaggactga tagaattttc tgtcgatcct agattttctt
    10021 gtaaaaaaaa cagacataca aaaatttcga taaaatagag tcgtctaaaa catcaatctg
    10081 aaaggaagat tttgtatatc catttctcgt tctatcaaag agcacttcac tattaaagat
    10141 aaaaatattg aaattaattc ttttcaaaaa actttttata aggacaagaa agccatcgta
    10201 atcgaaggta cattagttcg cacttttaga cattgtctat tttgtaaaga aactgcgaag
    10261 cagctcgtga aaaacggcaa gaagatttca atgattatct taaatcgcag tgggaataaa
    10321 taaacctatt tgcgtcttag aaaacaacat taccactacc aatcatgcgg aaaatatttt
    10381 actgctcata cgtatcttgt cgttccttca tgttttattt cgaaacaagt tcattacacg
    10441 atattagaag agttgaccga gcgtcaagcg ttaaagacac ttacgaaacg ttgtgatctt
    10501 tcagtaacga catttcaacg gacattatct gctctatcgg caatgctgcg acagatgcta
    10561 gactatttac ctcattgttt actattcgac gagtttcgct ctctttcaat ttcccatgga
    10621 aagttctcct tttcttgtat gaatagagag acagggaagc tctttgacat tttgtccagc
    10681 cggagaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc tcatatgggg
    10741 gttcgataca ttgtcacaaa tatgaatgcc ccttattttt cattagtcaa agagtccttt
    10801 ccaaacgccc aaatcatcat tgattgcttt cacattggac aacacttgaa caagtttttc
    10861 gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aagacacttc acaagctaag
    10921 tactatcgac aattgaaacc tttgtataaa ttacttctaa aatcagatga cgagttggat
    10981 tacacaacat tcaagaaatg gcagaatttt caatggcgct atttaacaga aagtgaagta
    11041 gttgatcgtc taccctcaat ctcttatgaa cttaaaattg ctaagcagtt tatcaaaagc
    11101 tgcttagcaa ttatcacaag aaacagctag ctacttcgag aaacgaccaa agaagttccc
    11161 tcgaaacttc actatctcaa aaagcctttt tcatatctga aaaaggcttt taactagcat
    11221 tttcgaaaaa gtattctaat aacaaaatta gagaatcttc gcacacatat caaaacattg
    11281 aaacgatttg cttatgggtt tcgcagtttt accaatatgc gcttgtggat ttttctaatc
    11341 aacaggttaa tcagctatgc attaaaagct agtaactctt actaactttg ttgttcataa
    11401 atgaccttat cagcccgatt tgacaaagaa ctactttttt acaacattat tttattttcg
    11461 agtaatcttt cttatttcat ttcgtatatc tggaaaaaga aaataaacaa acggaagata
    11521 aataatgaaa atcattattg atttaattgc catatctaaa aatgaagaag agaataattg
    11581 tggaagaaag taataggccc aagtagtcaa agtaagattg actataaaaa ataagataac
    11641 gttcttccca aagaaatagg atatttttat ttcttttcgt acaaaaaata ctctaaagaa
    11701 aaagactaat aattctacta ccacgagtac gatcgtagcc gctacagcgc cataagtagg
    11761 cacaaaagct acatttccaa taatacttac aatcgcacca atgatcattg gataagcata
    11821 ctccttatct ttttctatag caattgcaaa ttggttagca aaaactccac caattggact
    11881 aacaactata attagacaag aaataagcat gtttattgtc atcggtttga aattctcccc
    11941 aaaaaaccat ggaacaaatt cttttgtatt gataaacacc acactaaaaa gaattaatgc
    12001 aatgataatc gtgtaatcaa aagatttttt tgtgtaaact aaaatatttt ctgatttccc
    12061 ctctgcctgt gctttagata ttttaggcaa cataacaata gtaattgatg ttaagactgc
    12121 caatataata cgagcaattt tttgagactg atcataataa gataattccg ttggatccac
    12181 aaaataccca actatcgttt tgtctaaagt tgtgtacact tgcataaaca actgaggagt
    12241 caaaataata aaagctggtt tccacaactg tttaaaaaca ttagagttaa agattggttt
    12301 acctactttt ccgtggatac ttaaccagaa aactaaactt cctaaaaagt tagtaattga
    12361 agtaatgaac acgtaaatcc acaaatcatc tttagttgaa acaaaaatga ttattaacaa
    12421 taccgatgat aatttgataa ctgtatttct aataaccact ttcgaaaatt cttcaattcc
    12481 ttgaaaaaac caagaaatat ctaatgttag ccctacaata aatatcaatt gaattaagta
    12541 taaattttta tactttacat caaaaaaata aatataggct atatagagaa aggaaacact
    12601 caagcctatt aaaaattgaa taaagtaaat attccagaat tgtaaagata gtgctttttt
    12661 atctttcctt gatttagcga tatttcgaat acgaaaagga ttcattccca cagcaatgac
    12721 agtcattaaa aactgtgata ttgagaaaac atacccataa atccctaaac cagttgaacc
    12781 aattctacga ctaagtatag gtatagtaag taagggtaaa acaatcaaaa agacttgata
    12841 tatcgcatta taaaatatat cttttactgt ttttttcact ttatatccct ctctatcaac
    12901 tcaagaacct tttctgtact tgctcctgtt tcataatttc caatataatc aagtaactct
    12961 tgagaacttt tcaaaagctt ttcttgttgg tttttaaaaa catcaacaag gccataacta
    13021 tcttgtacgc ttataattgg taactcactt aactgtatat aaaaacctct ttctaagcta
    13081 tattcttttt catcatagtt aaaaagaata attggttttt ttgttagcgc aaaatcgaaa
    13141 aaaatagatg aataatctgt gattaaaaca tctatctctt caaaaagcat ttcaagagga
    13201 atcccatcat taattaaagt aatttgatgt tttttcatat attctatact tttagaatca
    13261 gttgctaatt tactatgaac attaggatga aatttagtca taaaatttaa ttcttgttgt
    13321 tgctttttaa aaaattcacg taagttatcc acacctaacc attctagttg cgtaattccg
    13381 ttgtcctcaa tattatttcg aaaagttgga gcaaataaga cattgatttg tttctttttc
    13441 aagtcaaaca cttgctcttt actagaggct aattcatcgt ttctaggagt tcctacttct
    13501 aatattttag aaggaacgat attgaatgct gaaacatact tcttagaact atagcgatta
    13561 ccagataata aatatgtaat tgactcacta tcttttttcg catagcttag gtaacgttta
    13621 ggtaacttat cttttgcatc aaattcaatt ttttttaaag gcgttccgtg ccatgtttga
    13681 aaataaagtt gttttttcct ttttgcatac ttaatcgttt tccgactatc gtctatccaa
    13741 tacttagctg tatataaata aaaggcagct aaaaaagaat cggagtcaat tcttttaata
    13801 taatctggta aatcagttat aatcgcttct tttttgatta accaaataat tctgtaattc
    13861 aatttttttt catgaatttt ctctgaaata actcttggat ttccagaata gttacgcccg
    13921 ccaaaacttg aaaatacaat taaatttttt tgaataggta atattttaat aaaaaccggg
    13981 aaaatattcc ttgaaaaaaa
//
LOCUS       DAXLPI010000066        13969 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXLPI010000066 DAXLPI010000000
VERSION     DAXLPI010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13969
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.066"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACF3T5_001730"
     CDS             complement(325..2322)
                     /gene="tkt"
                     /locus_tag="ACF3T5_001730"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFD3632333.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPIGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2424..2675)
                     /locus_tag="ACF3T5_001731"
     CDS             complement(2424..2675)
                     /locus_tag="ACF3T5_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFD3632334.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2822..3445
                     /gene="lexA"
                     /locus_tag="ACF3T5_001732"
     CDS             2822..3445
                     /gene="lexA"
                     /locus_tag="ACF3T5_001732"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFD3632335.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3624..3842
                     /locus_tag="ACF3T5_001733"
     CDS             3624..3842
                     /locus_tag="ACF3T5_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632336.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(4130..4642)
                     /locus_tag="ACF3T5_001734"
     CDS             complement(4130..4642)
                     /locus_tag="ACF3T5_001734"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFD3632337.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACF3T5_001735"
     CDS             complement(4656..6953)
                     /gene="recJ"
                     /locus_tag="ACF3T5_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFD3632338.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLMHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPVE
                     FLDLVAIGTIADLVSLTDENRILVSLGINAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSN
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     IMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(7095..7538)
                     /locus_tag="ACF3T5_001736"
     CDS             complement(7095..7538)
                     /locus_tag="ACF3T5_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFD3632339.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7539..8327)
                     /locus_tag="ACF3T5_001737"
     CDS             complement(7539..8327)
                     /locus_tag="ACF3T5_001737"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD3632340.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETDFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACF3T5_001738"
     CDS             complement(8324..9265)
                     /gene="rnz"
                     /locus_tag="ACF3T5_001738"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFD3632341.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9442..10029)
                     /locus_tag="ACF3T5_001739"
     CDS             complement(9442..10029)
                     /locus_tag="ACF3T5_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFD3632342.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACF3T5_001740"
     CDS             complement(10250..11563)
                     /gene="obgE"
                     /locus_tag="ACF3T5_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFD3632343.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11851..12861
                     /gene="gap"
                     /locus_tag="ACF3T5_001741"
     CDS             11851..12861
                     /gene="gap"
                     /locus_tag="ACF3T5_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFD3632344.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12946..13881)
                     /locus_tag="ACF3T5_001742"
     CDS             complement(12946..13881)
                     /locus_tag="ACF3T5_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD3632345.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3856 a         3130 c         2455 g         4528 t
ORIGIN      
        1 gagcaatcgc ttttttattg atcaccattt ttcttttagg tctatggtgt cgggcactgc
       61 gggtcttcag ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc
      121 tcttcttaaa acaaacttaa ttaattaaat tatactttta attctcaaga aacacaaact
      181 aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac gaaaagtaat
      241 attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga cagctgttct
      301 tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca cgacattttc
      361 tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac cgaaatgatc
      421 gattgtaatc gttgcgccct ctgttcctac gtagcgttcc caaccaaatg atgcagcagc
      481 ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat attcagaaga
      541 ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt tgcctttttc
      601 agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg caatcaaaat
      661 accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt tcaccatttc
      721 atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca atacaaggat
      781 agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt catttccgtc
      841 tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt gttcaatcgg
      901 ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga cgtaaacaac
      961 aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga caaagacaaa
     1021 gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca tagccgaagc
     1081 cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt caggagtgaa
     1141 atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg aaccacccca
     1201 gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa cacggcttgc
     1261 ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg gtagtttgcc
     1321 atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat aattggcaaa
     1381 catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt gatggaagcg
     1441 ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa cttctttagc
     1501 catcttgatg ccatcttcac caataggagc accatgaaca gcagatgttc cttctttagg
     1561 agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg tttcagcttt
     1621 tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca ctaagatatg
     1681 ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt ttgaagttgg
     1741 accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta gtttcatatg
     1801 acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt caccgcatat
     1861 tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg ctgcaaggtg
     1921 cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg gccctgtagt
     1981 ggcttctaca ccatctgtgt gatgtacttc aggatgtcct ggtgttttag aatcccattg
     2041 acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa gcaagctata
     2101 caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag cccagttttt
     2161 tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca taggtgctgc
     2221 gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa tacttaatgt
     2281 acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc ctcctattaa
     2341 tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc acacgaaaaa
     2401 gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa gccttttttc
     2461 ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc gacgactttc
     2521 attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc tttgcgtaag
     2581 ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt acgtgcaagt
     2641 tcattgatcc tctctatttt ttcaggtgac agcatgctca gccctccttt tactctcctt
     2701 catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat acgaacattc
     2761 gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga aggtgtacct
     2821 tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc atgaacaagt
     2881 aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc agctttcttc
     2941 tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta tccaaagaga
     3001 tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga ttgggattcg
     3061 tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt tagcagtaga
     3121 ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag gcagtctctt
     3181 tatgctgaca attcgtggag atagtatgat caatgctggg atcttggatg gtgacaacgt
     3241 gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa tgactgccga
     3301 ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc tgcagcctga
     3361 aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac gagtagtagg
     3421 actttatcgt agtcatatct attaattgct actaataaaa gaaaactttt taaaaagagt
     3481 ttgagacgat ggtctcaaac tcttttttcg cccgggttct tcttctttca aatttttatc
     3541 acttccctct ttacaaaccg aacaaatatt cgtatactaa atatacgaac aattgttcgc
     3601 ataaattaac gaggtgagaa ttcatgaaat caatgagacg tcttggctta gtattaactg
     3661 ttttgttttt aggtattatt attggtaatt tcggcatacg tccagcttta cttgtgtttg
     3721 ttccgttatt tactttgtgg ttcatgttgt gggatgaaaa gaagtaccgt caatctgagc
     3781 ggcgcagaat tcatgaagat catttctatc atgagcccta ctacagatcc aggcaatcat
     3841 aataaggatt gactgctaat tattcaaaag acaagaagtt aaacttttac gggggtgtga
     3901 caaaaatcgg tcacatcctt ttttcgaata aacggtgttc taaagccagt ttttcggtat
     3961 taagtcaaat tgcggaaaaa gtgatttttt aaacttctac tggtctttgc ttgtttccat
     4021 ttataaaaaa atagcgaccc tcaaaagtca gatagagaat ctgacttttg agggtcgctt
     4081 taaaatcgct tctttaagaa taacatcaag aatagcttct tttttctgct taatattcca
     4141 ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc atggagatcc atcaattcga
     4201 tcaaaaatgc gcagcctacg actacaccgc ctaattgttc gatcagttcg atcgtcgctt
     4261 tgattgttcc tccagtagct agcaaatcgt cacaaatcag cacacgttga cctggtttaa
     4321 tggcatcctt atgcaatgtc aatgtatctg ttccatattc taatccataa gtcacttcga
     4381 tggtttctcg aggtaacttc ccttttttgc ggactggggc aaaaccgact cctaactcat
     4441 aagcaacagg acatcctacg atgaatccac gggcttctgg cccaactacc atatcaattc
     4501 gtttttcttt tgcataattt acgatttgct tcgtagcttc ccgataagcg tcaccatctg
     4561 ccatcagtgg ggaaatatct cgaaacacaa ttccttttga cggatagtcg ggaatactgg
     4621 caatataatc ttttaaatcc actctttgtt tcctcctagg ttgttaacca tttctttatg
     4681 gtaggaatat cgctcaaaag caaaaattcc tcaactttga ttttattgag ccgccgctgg
     4741 tataatctgc tctcagtcaa aggatgactt tctgggctag cgactttttg taaaacaccg
     4801 ttctctattg taacaaattt caattcaaaa aacacctgta tcataaaaat taataatttt
     4861 tcagggatac gcaaatattg agctattgct tttattttat gacgtatatc aattcgttct
     4921 tgagaatgga tcagtttaaa caattttgcg tactgttctc ttgtgcctac cccattcaaa
     4981 taggcctcat caggcgaaat ccccatcaga taaacacgat tgaatgaata agaccgaaaa
     5041 acttctttca gatccgctaa atcatctgga caatccataa taactagaga gttcggtttt
     5101 cttttttcaa ttattgcctt tacagaatct agtccttcga aaacaatcgc agcttcctgg
     5161 ataatctcag gcacgaattt caacgtagaa gaatcaaatg ctaaataaag gctaccttcc
     5221 gacgttcggc tctgttcacg gaaacgtttt gcccgccaat caaatacttg cagaccaccc
     5281 acagaaaaat cagataccat cagctgcggt ttcttacgcc cgttccattc attgatcgaa
     5341 agctttccta ccacatctac ctgattattc agcagttctt cttcttgagt accaaaacca
     5401 aaagcgaccg catcaagctg actcgatgca ttggaaagga caaatttcag atgctgttgg
     5461 tttgctccga ttcttttgat attttctgct tggacttgtc gaaataaaaa ctgaggcaaa
     5521 ggattatccg taccaaatgg cgccaatagt ttcagctcat caattcgctc tactgttact
     5581 tcatttggca gcaatacttc atcaattctc aactcaggcc ctctcatcaa gtcaatttga
     5641 tgatcgacta tatattggtt catcttctcc tgtagtatag tgacattttc acttggcatc
     5701 gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa ataaatctcg catctggtca
     5761 agcatttcaa aaagattcaa ggcttcaata ctacgtccag atcctttagc tgatccatct
     5821 tcttttaaag taaggacaag cgttggttta cccgtttcat tcattatttt cccagcaaca
     5881 atgcccaaaa cgccttcatg ccatcctcgg tttaccaata gatggatatg attctcctta
     5941 ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga ttgcttttct ttcttcattg
     6001 atctcattca atttttttgc ttgaacggat gcttcctctt catcaaacgt cgcaagtaac
     6061 gagacggcag gacgtggatc gcccatccga ccgattgcat ttagtctggg agcaatcgaa
     6121 aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc cgctttcttc aaatagcgcc
     6181 tgaagtccta tccgttctga atgatgaatg gcattgattc ctaatgaaac aaggattcga
     6241 ttctcatcag tcaatgaaac taaatcggcg atcgttccta tcgctactaa atccaaaaat
     6301 tctacaggcg gttcttccaa taatgcagaa gctactttga atgctactcc gactcctgcc
     6361 aaatctggaa atggatagtg ccccgctgga tgacgagggt gaacaatcgc ataagcgtct
     6421 ggtaaattct caggtagttc gtgatgatct gtaatgatca cgtctacacc agcttgctga
     6481 gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa cagtgatgat caactggatt
     6541 ccttcttcga tcttttcttg gtatacagat tggtttggtc catacccatg ctcaaatcga
     6601 tttggcagga atacttctac ttctgcacca agtaattcca gtgtttcttt cattaccgtt
     6661 gcactggtga tcccatctgc gtcataatca ccgtaaacga ggattttttc tccactgatt
     6721 acagcttctt ggattcggct gattgcttta tccatatcgt gcatcagata cggatcatgt
     6781 aattcttgtt cctttggatg taaaaatcgg ttgatatcct tttcctcatg gtaacctctt
     6841 tgccacagta acttaccgac aaatggagat aatttcagtt cacggatcat ttggagaaag
     6901 gattcagaag gctctgtctc gcttaacttc caatcaaaat ttgcttgtct cactttctca
     6961 ctcctaactt agttattata gcaaaggaaa tcagcaaaaa aaagcagaaa gaaagaataa
     7021 aaacagatca gatccgctag acagatgttc agcaggacct gatcttttgt acgaccattt
     7081 atgaaatatc cctattagtc aaaataattg aagctttgtt tgggcgattg agttttttgt
     7141 tcttccggcg ctttcaattg actgatttgt gcggttttcg cttgtaactc cgcctgataa
     7201 gcagcttcca actccgcacg ttcgtttcta aactcgcgtt gctgttcttc ttttgcttct
     7261 tcaattttct tttgaagttc tgtttgatat tctgacattt cctgagtcag ctgtttgatt
     7321 tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa atacaatcag tgcaccaatc
     7381 agcgcagaac cgatgatcac taagatcaat ggcgcagaaa aggaagtgaa gccaaaactt
     7441 acaggaacat ttttattgtt caatacagca aaaagaacaa taatcaaaac taaaacaaat
     7501 ccaagaatca cattgctttg ctttttcaat tccttcacct actttttgtt aaaaattccg
     7561 ctggctaaat aatcaccaat cgttggaaac agcgtataaa attttgatgc aacttctaaa
     7621 ataaacggtt gattgatttc ccgttttgga tgtctcatat tgcggacgat catttttgcc
     7681 aacttggcag gttctaaaac gatttgtccg actttttcca aatagcttcc gcttggatca
     7741 gccttatcga agaaatctgt ctcgattgga cctggattca ctgtcgtaac agctacgcca
     7801 agtggtttca attctaatcg caaagcgttg gagaagccaa ggactgcaaa tttcgtggca
     7861 gaataaaccg ttgattttgc agttgccatt tttccagcca tagacgcgat attaatgata
     7921 tgaccttgtc ttctttctgc catatcgatc gcaaattttt gtgtcaaaac catcattcct
     7981 aagatgttga cttcaaacat atcgtatgct tttccaaggt cgaatgtaag gaagtcttcg
     8041 aaaatcccaa aacccgcatt gtttaccaga atatcgacgc gcccgacttc ttccatcacc
     8101 ttttcatata attcgtccac gctttcagga ttgctgacgt ctaccttgaa agcataagcc
     8161 tctttgccgc tcaagcgtct gcactcgtct ctgacacctt ctacaaatgc cattcttcgt
     8221 gcgcaactta taacgattgc tccttgcttt gctgcttcat aacagatttg tgcgcccaga
     8281 ccgcctgaac tacctgttac taaaacaact ttatcttcta aattcatgcc tcttcctcct
     8341 cgcgaaatgg aatctcaatg ctatcaaaat ctttcatgat cgtcgtgttt tcaaacactt
     8401 cttgtgcttc tttttcaagc tctaaagctg cttttcctaa atatctcgca ctgatatgag
     8461 taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc agcagcttga tgagtcgtgg
     8521 aatggaaata gttgtgcgcc attcttgctt catctttatt aaatgtgctt tcatgtacca
     8581 gaatatcgct attttccgca agcacaacgg aattatgtgt ttttctcgta tcgcctaaaa
     8641 tagtcacgat tcgtcctttt ttatctggac ctacgaaatc ttttccattg atcgtccgcc
     8701 catcttctag ttggatcgtt tcaccttgct tcagtttgcc gtacagtggt ccagctggaa
     8761 tacctaattc ttttagacga tccacctgca attctccttt atgatcatgt tcaaccacac
     8821 gataaccaaa actgtcgatc ccatgattta gtttttttgt ataaacagaa aattgctgat
     8881 cagtaaaaat cggggctgtt tcatttagct caatgaattt cagcggataa cttaaacgcg
     8941 tttgcgaaat acctaaagag acttttatgt attcctctat cccttttggc ccgtaaatct
     9001 ctaatggcgt atccccacct tgaaatgacc ggctgctgat gagcccggga agtccaaaaa
     9061 tatgatcgcc gtgtaaatgt gtaataaaaa ttttaccgat ttttctcgga cgaatagtcg
     9121 ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa caaccaaact tcattgcgct
     9181 cgtctaaaag tttcaatgct atacttgtca catttctatg ttttgcagga acacctgcac
     9241 ctgttcctaa aaattgtaat tccataatct acctgacttt ctttaagttc ctctttaatt
     9301 ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa tgggttcaaa ttaaaaataa
     9361 cgttctaaag cgatagcgtt ttctttgaaa attactataa aaacagcagc gaacccgttt
     9421 gagatcctct gctgtttttt attaatttga agatccagaa ctttcattgt cgttggaagt
     9481 ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct ttcgcatgat tttcagcttc
     9541 tttcgcatca tgcacatctt tttcgacatc ttcataatcc aagcgggtaa tttctccgcc
     9601 taattccatc attaccaatt gattcagtgg gcgattgtcc tcttcatcag caatcaagat
     9661 cagtcctgta gctccttcac cgattttatt tcctacaaag tcgaacacgg tctgggcatc
     9721 ttttatttct ttggcatctt gtgtcgcacc gatcattcct ccggcaaacc atcctaataa
     9781 aatccctagc ggaccaccta atacaccaac caacattccg attaatccac ccgtagaaga
     9841 tttgttatta ccggtaaagt cgataaaatc ttctattttg aattgatgtg tcccattagg
     9901 atcatggctg acaactgcca tctgttctcc tttaatcgaa cgaacagcat gtaacttttt
     9961 gatttctgaa aatgcctgat atgcctggct ttctacttga aaatttaaaa tgattactcg
    10021 tttgctcatt aaaaacgccc ctttcatacc ttcttgttat ttattttacc tttaattgta
    10081 aaataagaaa agaaaagcac tctttttttc caataaaata gggctgtgac aaaagtcgta
    10141 tcacagctcc tttttccgaa taaacggcgt tcttgaagct gatcacttct gtcacaacct
    10201 cccttaaatc tgaatctata ttttttggta tttgttttta tttgcttgcc tattcgacaa
    10261 attcaaattc aaactcaccg atacgtacaa ggtcgccatc tttcgcaccg cgtgcccgaa
    10321 gagcttcatc cacacccata ccacgtaatt gacgagcaaa acgcatcact gtttcatcat
    10381 gatcgaaatt cgtcatttgg aataattttt cgatcttctc accagataag atccacgttg
    10441 catctgaatc acgatcgatt tggaactcag gaccttctgg attgaatcca tagtgtacag
    10501 tttcttcttc cagttcttct tcgtacaatg ggaattctgg cgttacttct aacaaatctg
    10561 ctgtagcatt caataacgcg tccaatcctt gtcgagtcac tccagaaatt ggaaatactg
    10621 ggatgtcatc agcaaattca tcttcttttt ccttgttcag ctgttctttg aatttcacta
    10681 gattttccgg tgcatccggc atatccattt tgtttgccac gatgatttgc ggacgctcta
    10741 gcaagcgtaa gttataagta gataattctt tgttgatggc aagatagtct tcataaggat
    10801 ctcgtccttc catcccactc atatcgatca catgtaaaat tactcgtgtt ctttcgatat
    10861 gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc tccttcaatc aagcctggca
    10921 agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac cattcctaga ttagggacga
    10981 gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga tgaaataacg gatagtaaag
    11041 tcgatttccc gacagatgga aaacctacaa gtccaacgtc tgccaatact ttcaattcta
    11101 gttcgatttt tcgctcttgt cctggttcgc cgttttcagc aatttccggt gcaggattcc
    11161 gaggagaggc aaaacggata ttccctcgtc caccgcgtcc accttttgcc acaactagtg
    11221 tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt ctccgcatct cggacagtgg
    11281 ttccttgtgg tactttaaca taggtatctt ctgaaccacg accgtgcatc cctttactca
    11341 tcccattttc acctggctgc gctttgaaat ggcgattgaa ccgaaaatcc atcaatgtac
    11401 gtaacccttc atcgacgatc aagatgacat cgccgccacg accaccgtct ccgccagccg
    11461 gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac cattccgtct ccgcccttgc
    11521 cagctttcac atcgatcgtt acttgatcta aaaacatgga cataattaag tcctcctatt
    11581 attcatttat ttatttgact gcctgaattt ccacaataaa caaaagtctt acttatttta
    11641 tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga ttgtttacct tcttcttttt
    11701 ttatcatttg gtaaggtttc caaccttttt tctgctacca agtataattt tttgacaaaa
    11761 tattctttgc gtagcagaat aacagtgtat taattgtatt aattgaggat gccgtttcac
    11821 agcaccccta ataaaggagg aacttttgat atgacagtaa aagtaggtat taacggattt
    11881 gggcgtattg gccgtctggc tttccgccgc atcaaagaag tctcagatga tattgaagta
    11941 gtagcaatca acgacttaac aagtccaact atgttagcac aattattgca atttgactct
    12001 actcatggta cttatcctgg cacagttact gcaacagaag actcaatcgt agtggatggc
    12061 gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa tcccttgggc aaaagaaaac
    12121 ggtgtagata tcgtactaga atgtactggt ttttatacat ctgaagaaaa agcaaaagcg
    12181 catttagatg ctggtgtcaa acgagtagtt atttctgccc cagctggtgc tatgaaaacc
    12241 atcgtctata acgtcaatga tgatacatta gatgaaaacg acaaaatcat ctcagcaggt
    12301 tcatgtacaa caaactgttt agcacctatg gcttatttct taaataatga atttgggatc
    12361 gaagttggta caatgacaac agttcatgcc tacacttcca cacaaatgct cttagatgga
    12421 cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg ctgacaatac tatcccacat
    12481 tctactggtg ctgcgaaagc aatcggctta gttatccctg agctacaagg aaaattacaa
    12541 ggacacgcac aacgtgttcc agtcgttgat ggttcattaa ctgaattggt ttcagtattg
    12601 aaaacaaaag taactgctga tcaagtaaac gaagcaatca aaaaacacac agttgacaat
    12661 ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg atgtcattgg aacaactcaa
    12721 ggttcgatct ttgacccaac gcaaacagaa gtaacaactg ctggtgattt ccaacttgtt
    12781 aaaactgttg catggtatga caatgagtat ggatttactt gccaaatgat tcgcttacta
    12841 gaaaaattcg ctaacttgta atttgataga tcaatcgata aaagaagcct ttgactggat
    12901 ccagtcaaag gcttctttct tttcatcccc tacttttaaa ctcgttcaat gaacatattg
    12961 gcttaaaaat tgttggacta cttgctgata tttttcagga tctgtcttgt atgcttccgc
    13021 atgctcggct ccttttacaa catacttttc ttttggtccc ttcgttgcac gatagacctt
    13081 atctaacatt tcaaatggta caaatgtatc ttcgtctcca tgtataaaga gcattgggcg
    13141 ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg aaaaagtagc cagcacggac
    13201 ttttgtcact agactcgtta cttgcataag cggaaatgct ggaagactaa acatatcgtc
    13261 tagctgataa gcaagctcat catgaacgga agaataaccg caatcttcaa tgattgcttt
    13321 cacattatct ggtagttttt ctccgctagt catcataact gttgctgctc ccatgctgac
    13381 accgtataga acgatttctt gcgattttcc attgttttct agcactttgt ttatccactg
    13441 tacatagtct ttacgctctg gccatccaaa tccaatgtaa tccccttcgc tttttccatg
    13501 tcctcttgca tcaggaacaa ggacattgta acccatatca tgaaacatct ttgcgtaatt
    13561 ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca agagctgttt tttcactttt
    13621 cgtttcagca ggaagataaa tcgctgataa tttcagaccg tcttttgaag tgatggtcca
    13681 atcgcttcgg ttttcttttg aactaaacca tttatgtgca tccgtttctt tggatgtctg
    13741 tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca gaaggcacaa cagcataatt
    13801 ataaaaataa ttccctgcaa acactaacgc aacaatcaga gctaaagcga cgcctaaaag
    13861 gattttcatc caaagtttca taaatcatcc atctcatttc tcttttttca tataaccttg
    13921 aattagtata atcaaagaat ctctactatg caatgttcat ttttataac
//
LOCUS       DAXLPI010000067        13583 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXLPI010000067 DAXLPI010000000
VERSION     DAXLPI010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.067"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            73..234
                     /locus_tag="ACF3T5_001743"
     CDS             73..234
                     /locus_tag="ACF3T5_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFD3632346.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="ACF3T5_001744"
     CDS             253..1530
                     /locus_tag="ACF3T5_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632347.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="ACF3T5_001745"
     CDS             1540..2196
                     /locus_tag="ACF3T5_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632348.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="ACF3T5_001746"
     CDS             2196..3572
                     /locus_tag="ACF3T5_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632349.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3786..4115
                     /locus_tag="ACF3T5_001747"
     CDS             3786..4115
                     /locus_tag="ACF3T5_001747"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3632350.1"
                     /translation="MQDVLKDVFGPLFEKMLQGELNNHLGYDAHSKEPKKHDNRRNGY
                     GNKTLKTSFGKVNIDVPRDREASFEPELIPNRKRDVSDIADCRQKLFQTRNSSLEQLF
                     IYFVSNF"
     gene            complement(4038..5498)
                     /locus_tag="ACF3T5_001748"
                     /pseudo
     CDS             complement(4038..5498)
                     /locus_tag="ACF3T5_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            6132..7103
                     /locus_tag="ACF3T5_001749"
     CDS             6132..7103
                     /locus_tag="ACF3T5_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFD3632351.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(7080..7169)
                     /locus_tag="ACF3T5_001750"
                     /pseudo
     CDS             complement(7080..7169)
                     /locus_tag="ACF3T5_001750"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
     gene            complement(7181..7417)
                     /locus_tag="ACF3T5_001751"
     CDS             complement(7181..7417)
                     /locus_tag="ACF3T5_001751"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFD3632352.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(7386..7517)
                     /locus_tag="ACF3T5_001752"
     CDS             complement(7386..7517)
                     /locus_tag="ACF3T5_001752"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFD3632353.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(7593..7976)
                     /locus_tag="ACF3T5_001753"
     CDS             complement(7593..7976)
                     /locus_tag="ACF3T5_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFD3632354.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(8009..8794)
                     /locus_tag="ACF3T5_001754"
     CDS             complement(8009..8794)
                     /locus_tag="ACF3T5_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFD3632355.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            8972..10072
                     /locus_tag="ACF3T5_001755"
     CDS             8972..10072
                     /locus_tag="ACF3T5_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFD3632356.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10062..10886
                     /locus_tag="ACF3T5_001756"
     CDS             10062..10886
                     /locus_tag="ACF3T5_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HFD3632357.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            10883..11698
                     /locus_tag="ACF3T5_001757"
     CDS             10883..11698
                     /locus_tag="ACF3T5_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632358.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            11695..12525
                     /locus_tag="ACF3T5_001758"
     CDS             11695..12525
                     /locus_tag="ACF3T5_001758"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632359.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            12532..13563
                     /locus_tag="ACF3T5_001759"
     CDS             12532..13563
                     /locus_tag="ACF3T5_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632360.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
BASE COUNT         4290 a         2321 c         2868 g         4104 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg
     4081 aacagctttt tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct
     4141 ttccatagcc aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc
     4201 gtcgcgactt tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca
     4261 tccgcaaata ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct
     4321 gttaggcgaa gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatgact
     4381 cgttgatgat ttttacttat ggtacattgg tttagcagag gacagttcac acattctttt
     4441 ggattggatt tgtattctcg gtacccttct cgagtaaccg ttgaaaaagc gagcatttga
     4501 tcttgcggac aaatataaca atcaaaatac tcgtcataga gaaactcatt ttttgataat
     4561 agctcctttt tccctttggg ccttgaataa ggcaaacaag gtcgaaggtt caaatgagtc
     4621 aaaaagtgaa caaacttagg tgtcttatac ccagcatcag cgacaacaca attgatttca
     4681 ggaaaaagtc tcttcgattg tttgacgagc tgaaaggcga cttgactgtc atgaatattt
     4741 ctaggagtga tgattgtgct aagaataaat ccattgtcgt cgcatgaagt atggacagaa
     4801 taagcaaatt gtttttctcg ttcgcctttg acgtagtagc cgctttccgg atcagcttta
     4861 cttatttttc tttctttcag cttcacttcc ttttctaagt ccaaagtaaa agggcgtttt
     4921 ccagcctcta ttcgttgacg attgatctca tcttctaatt catcctgata ggctttcgct
     4981 tcatcatggg tcatttcttt cgtaaactta tgtttattgg cgttagcttt gatgtgggta
     5041 gaatccagat acaaattatc ctctgttaca aaacctgctt taactgcttg ttctaaaata
     5101 taggcaaaaa tatcctcaaa caccgtcgtt tctctaaatc ggcgaacata attctttcca
     5161 aaagttgaga aaggggggat tttctcctcg aaggagtagc ctaaaaacca gcggtaagcg
     5221 acattggtat cgacttcctt aatggtttgt cgcatggaac ggataccgaa taaatactga
     5281 ataaacacca atttgatcag aatcactgga tcgatacttg ggcgacctaa atcggagtaa
     5341 gtggcttcga cgattggata aataaattca aaatcaagcg cggcatcaat ctttcgaacg
     5401 agatgatctt gaggaactag atcatcaatc gtaagcatcg caaattgatt tctaccattc
     5461 gtattgtttt tagacatcat agctatcact ccttgcattc gttttcataa atctattata
     5521 aaagaaaaga ctgtagacat catccataaa atggactttg tctacagtct ggataacgtg
     5581 attttttgct gtaatgacaa ggttttagct gtttcagata ccatgtatcc aaaaatagtt
     5641 tattgagaaa ataggagagt atttgatact aactagtagg aaaaaatgag ataaaaagta
     5701 aatggaatca tttaaggtaa tctgaaataa aaaatataca gccctgtggt actttaccta
     5761 caaggctgta tatatttttt actctcagcg tttttgactc cgtgtctgac tccgttttat
     5821 tgattttgaa tgttattcaa tgaccattta acaggtcaaa tttgatgttt ttgattccgt
     5881 atgacgccgt ttgatatcta gtgatatagt gatttgagtc tacatcgaat aagggaataa
     5941 accaataaaa aatgatttag aatattaaaa taacaaaaaa gaggactacc tgtattagtc
     6001 ctcttttttg accagatttt gaccagtcta ttttacatcc tagtatattt atatgagcta
     6061 attttatgat taaatggtaa gtttttcctt gtgtgtttta gtttaaacta gtgtgtaatt
     6121 caaaaatcta tatggaagga acagtattaa gaatagaaca gggttctcta catgatggtg
     6181 ctggtttacg gaccgttgtt tatttaaagg gctgcccatt acgctgtgcc tggtgttcga
     6241 ttcccgaatc acaaagcaag cagattgaaa aaggatttgg ccagaccatg accgctgaag
     6301 aggtaatgga tgagattgag aaggatgctg ttttttactt tcactcggat ggcggagtga
     6361 ccattagcgg aggcgaggca ctggttcagg cggattttgc taaagaaatt ttgcaaaaat
     6421 cgaaatacat tggaatcaat accgtactgg aaaccagctt ttgtggtgct tataatgaaa
     6481 tccaaaaggt agctccctat gtggatacat tgtttgtgga tgtaaaaatg tttaccagca
     6541 agctccacaa acaatggacc ggcttagata accaacaaat tttgaagaat attcgtcgct
     6601 ttttaattga gtatcctaac tgcgaagtgc gaattcgagt tcctgtggtt ccaggtatca
     6661 atatgaacct gaccgagctg ctgacgattg cttgttttgt ggcagatctt gatcggttcg
     6721 taccgttaga attactaccg taccactgct atggtatgca cggttaccaa gcattgggac
     6781 tggagtatcc gttggcagac acgccagcgc cttcacctaa agaaatgttt gcattggcag
     6841 atcaactggc tcgcacggtt cctcatttgc tggtcacagc tttaaccaaa acatttattt
     6901 atttattgaa tccaagcttt atcttatgga taaatgaaag acactcacta gctgcagcca
     6961 gctcaagaat agagcttttg ctgaagtcag tgagtgtctt tttacgatgt tttttcgaag
     7021 ttaatttgtc gaatgaacac tggctcaatc ggtacatctt cactgtttgt cggctgagct
     7081 gcgatcgctg ctacgatttc taacccttgg tatacctgtc caaaaacagt ataacgacca
     7141 tccagctccg gtagtcctcc aagctccaag tatgcttgct ttacattagc aggtcagcct
     7201 gcgcctttgt gttttgcaca ataaaaaact ggttgccgtt ggtgtggggg ccgtggtccc
     7261 ctaaggccaa ggcgccatta aaatgataaa gtccgcgatg cacttcatca gcgaaaaagc
     7321 cctcatagct ggaaacagct tcatcaccag aaccatccaa agcgccacct tgaataacaa
     7381 attccttaat gacacgagcc gaaaggtgtg ttgtcataaa cgccttgctc agctagggag
     7441 cgccagttgg ctacagccag gggagcttgg tcaggaaata agcggagacg aatttcacca
     7501 gcatcggtga ccagcacaca aacttcttcc tgctgcggtt tttctaattg aaagagagga
     7561 aggtttgcaa ctgaaacgcc ttgatcctgc catcaaatat cgaaaatccg ccactcgacc
     7621 ttcgcctgat caccaaggcg agccattccc aataaaacgg tataggcggt ttcatccgct
     7681 tgaaaggtca attgcagctg tcctcgtaaa tcgttgtagt catctggggc ggctacttga
     7741 taaaaaagat gaaactgcgc atgcaaatat tccaaaggct gatctttaaa ggctgcaaaa
     7801 taagcctgaa ctttttcccg ctgcaagctg tcagccacca aaaaattttc cagttgttgt
     7861 tttggaacgg ctgctaacaa ggatcggcaa accgcctcaa ttttcaacgc atcccaatgt
     7921 cgcgcaatta aataatggat gggtgcctct tgattgtagt cccacaggtt gctcattttt
     7981 ttcaccactt tcttctatta tataggaatc acaaaatcgg cagcggcgtc aggcggcgtt
     8041 cttttccttg aatgactagc aacttttcta cctgacagtc tgccaacagc tgactcattt
     8101 ttttaaagcc cagctgataa tcttcggcaa catgagcatc cgagcctaag gtaaagagct
     8161 ttccgccgac actttgatac aaaggaacgg cataacggta aaggtcttcg ttgccgtatt
     8221 tcaagaagct cttggcattt aactccatcg ccaattctcg ttcaacaacc tttttgaaaa
     8281 tcgttagcag ctgagcctca aaatgctccg cgaattcttc cgctgtgaag gtgaagcggc
     8341 gtaatccata atcaaagtga gtcaaaatat ctccttctgg aaaatccgtc aagacctgca
     8401 acatctgatc aaaatattcc ttggtagttt gaatgggatc tttggttaaa accacatcgt
     8461 ccatataatc aaagcggcca ttttgatgaa tacttaccag ctttaaatca taaggatgct
     8521 gagcgaggta atccacgatc cgctcttctt gtcctggaac aacgccaatc tcgatacctc
     8581 gcagaaattt ggtggaatag gtttctttta aagagtccag tttgtttata taggctggat
     8641 aatccggaat gtcatctttt ccggtagatg gattgtttaa atcaaagtga tccgtagaaa
     8701 cgaaaaactc cggctgatag gctaaatagt tttcaaattt ttcctctgaa tcaaaggaaa
     8761 aatacgtgtg gagatgctga tcataatact gcatagggag cctccttttt tctactatca
     8821 taccgcaaaa gctttagaag gttaacggga attcgtcgaa atttacaaac catatacaac
     8881 ctttacattg ttcatgaaac cttttcaaga aactgattct ctcattacaa agctttgtta
     8941 gaatggaatt atcgaaaggg gagaagaatg aatgaaaatg aatgaaaaaa ttcgtaacat
     9001 gactatcggt ttattttgtg cagcaacagt aagtatcctg ctgacaggct gcggctcagg
     9061 tgaagcaacg aagcaatcgg ctgcggcaga cgactcttta gatacgatta tagaaaaagc
     9121 caaggaagaa ggagagattg ccagcttggg aatgccggat acttgggcaa attgggtagg
     9181 cacttggaac gaccttgaaa aagaatacgg cttaagtcac gtggatacgg atatgtccag
     9241 tgcagaagaa ttggctaagt ttgaatcaga aggaaaaaat ggaaccgccg atattggtga
     9301 tgtcggtatc agcttcggtc cgttggctga aagcaaagag ttaaccttac cttataagac
     9361 caactattgg gaggaaattc cggattgggc aaaggatgat gatggggatt ggttgttaag
     9421 ctatacggga accatttcct ttatcaccga taaagagaat gtgaagcaag caccgactag
     9481 ctggaaggag ctgcaagagg gtgactatca agttagcgtg ggggatgtga cgattgccaa
     9541 ccaagcacaa tttgctgtat tggctgcggc gattgccaac ggaggtgacg aaaaggatat
     9601 tcagccaggt ctgaattact acaaaaaaat ggcagaaaaa ggccgtttgt ccaccgttga
     9661 tgccagcgta gcaaatcttg aaaaaggtga aatcgatgtg gcagttgttt gggacttcaa
     9721 tggactaaat tatcgtgacc aaattgactc cgagcgtttt gaggtaactg tgccaacaga
     9781 cggctccatc attagcgggt atacaacctt aatcaataaa aatgcccctc atcccaatgc
     9841 ggcaaagcta gcacgggaat atattttatc tgatgaaggg caaatcaatt tggcaaaggg
     9901 ctatgcgcga cctattcgca aggtagagct gcctaaagaa gtagctgaaa aattactgcc
     9961 ggaggatgct tatgaagcgg cggtgccagt tgaagacaat gaagcttgga acaagacctc
    10021 tgtagagctt ccagagcttt ggcaggcgga ggtactgacc tatgcaaagt agacttgaca
    10081 aactgattgt agttatacta gacggctgtc gctacgacac agccgtcgcc cagcttgggt
    10141 tcatgaatca tttagttgag caccagcaag caagcttatt gaaggtaaaa agtgagctgc
    10201 ccagcaattc acgtccgtta tacgaagtac tgatgaccgg cgttccagcc tatgaaaatg
    10261 gcatctatag taattaccat gctcagcgat ccaaagagtg gtcgctgttt gaattagtta
    10321 aggaagctgg cggaaaaaca ggagccgccg cttaccactg gtacagcgag ttgtacaatc
    10381 aagcccccta taatcgtttg gttgatcgca tccagcatca ggaggagcga ctgattcagc
    10441 atggcatctt ttatagtgag gacacttacc ctgattcgca tttatttgcg gatgcccatt
    10501 atttagtgaa tcagtatcag ccagactttt tggtcgtaca ttcgatgaat attgatgatg
    10561 tcggtcacaa atttaccgcc gattctcatg aatatgcagc agcggttaat caggcggaca
    10621 gattattggc tatttacctt cctgaatggc tgcaaaaagg ttatcaagtc gtggtgactg
    10681 cggatcacgg gatggatgcg cacggactgc atggcggcag cttggctgca catcgtgaag
    10741 ttccttttta tttgatttca aataaacgcc aaaagctgaa ggaacaaact gtaccacagc
    10801 tgctggcggc tccattgttt tgctggttat taggaattgc accgtcagag aaaatgcagg
    10861 acattcaaga attgatgagg ggatgaaaga gatgaaaaaa cgcttggttc tggttcctgc
    10921 ctcctttatc ttctgtgcat ttttactgct gccgctgctt tttatggttc tgtgcagctt
    10981 gcacagtgat ttaactaacc agtggacatt ggccaattac agtgatattt tcagcaacaa
    11041 atattacaca caggcctttg ttaacagcgt actgattgct gtcagctcca gtgtgattgg
    11101 gttaatcggg acgtttattt tatgtttatg cttaatgaat ctatcgacta aattgcagga
    11161 gaaaatcacc tttgtttcca atttggcagc taattttgct gggattccgc tggcattctc
    11221 ttttattatt ttgttgggga atgtaggtgt gctgaaaatg attttgccat ttctagcgga
    11281 cttcaatttg tattcttggt ggggattgac catcacgtat acctatttcc aaattccgtt
    11341 aggggtcttg tttttgtatc ccagcatgaa ggaagtgaaa aaggaatggc tggagtccgc
    11401 acaattgctg ggcgcttcat cagcgtatgc ttggttcaag gtggctttgc cttttttgcg
    11461 accatcgatt gccagcacct ttgtcatctt atttgctaat ggcatgggga cctatgaaac
    11521 agcgtatgcc ctgaccagca gtaatgtaaa tcttttaacg attcgaattg cggctttagt
    11581 gtcgggagat gtgttcgcca agcctaattt aggcagtgcg ttggcagtcg ctttaggtgt
    11641 cctgctggta acggcaatgt tgattattca aaagaaaggc aaggtggtgc agacgtgaaa
    11701 aagttaataa aaccgatttt gttctgcttt gtcctgtatt acttgctgcc gattattgga
    11761 acgattttgt atgccagctc cactaaatgg tcaaaatcgc ttttacctag tgattttacc
    11821 ttgcaatggt tccaacagct gctgacggac agggaattta ttgctgcggt gggtcgctca
    11881 cttttattag caggtgtggt cttggtgacg attctgttgt tgatgattcc caccattatt
    11941 tggattcatt tgtatttccc tcgtttgaat cgttggctgg aaaagctgct cctgctgccg
    12001 tatgctttac caggagtgat tttggtaacg gctttgcttc gaacgtatgc tgaaactggt
    12061 atcccaatgt ttgtagtgtt ggtaggtgca ttgtttatta cggcgttgcc gattgtctat
    12121 cttagtctta acaaccagat gcgcttaatt aatctgaagg aattagtgga tgcagcagaa
    12181 accttgggcg ccccgatgtc tacgattatt atccaggtgc tgtttccaaa catccgtatc
    12241 ggtgtgaccc tagtttctct gatgattttt tcttcggttt ttggggagta catgctgacg
    12301 aacctgttga ttggcggtcg atttcagacg gtacgcatct atatgctgcg gcggatgaac
    12361 gaaaacggcc atttggctag cgctgtgatg gttttgtatt tgctgtttat ggtgggaatt
    12421 gctttaacta cctttatgct gacttatcga caaaagcaac agctgtccct taagcagacg
    12481 agggaagaga agcagtcgaa aaagaagccc ttaaaggagt tgtgacggaa aatgttttta
    12541 gaaatacatg atctcaatct gttttttgat gaaaaacaga ttttagctat tgatgaaatt
    12601 cagattgaaa agggtgagct ggttacttta ctaggtccca gcgggtgcgg aaagtctacg
    12661 ttgctgcgct gtatcacggg attggaacaa ccaaagagcg gtgagattgt gctggacaat
    12721 gagaatattg ccgataagcc tacgaaggat cgcaatatcg gctttgtttt tcaacagtat
    12781 gcattgtttc caactatgac tgtttttgaa aatgtagcct ttggtttgaa ggtaaagaag
    12841 ctgagtcctg aggtgattca ggaaaaggtt tttgagatgc tatcattggt ggatatgacg
    12901 gagcaggcag ataaaaatgt ccagtttctt tctggcggtc aacgacaacg ggtggcactg
    12961 gctcgctcat tggtcactga accgaaagtc ctgctgctgg atgagccatt aagtgctttg
    13021 gatgcccgaa ttagaaaaca gctgcagcgg gatttgcgcg ctatccagca atcgctaggg
    13081 atgacgatga tttttgtgac tcatgaccaa gaagaggcga tgcgaatcag tgatcggatt
    13141 tttgtgatgg aagctgggcg ggtggctcag gtttccacct cgaaggaatt gtatcagaat
    13201 cctcagagcc gatttgttgc ggaatttatc ggcaactata atcgtttcga atggtatgag
    13261 ctgaatcagt atacccacga gttttccgct aagggcaatc gctgcattta ttatttaaga
    13321 ccggagctga ttcaatttga accggttcct cagggggtta agattccggt tctatggaag
    13381 gaatcattta ttttaggcaa cattcaacgg tatgtttttc aaacgctgga gggcaaagaa
    13441 attttagtgg atcggttgaa cgatcgggaa gagaaaatta gcgatgcctt atacattcag
    13501 ctgaaagata ttctagcaat ccctgtggaa attgatactc agcattttgc acaacaaagc
    13561 tagtgtatag tcggcaaaca ttt
//
LOCUS       DAXLPI010000068        13305 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXLPI010000068 DAXLPI010000000
VERSION     DAXLPI010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.068"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..311
                     /locus_tag="ACF3T5_001760"
     CDS             <1..311
                     /locus_tag="ACF3T5_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3632361.1"
                     /translation="IHFARREKKLYRSLYLEEYGGGKEMQNFSYTYFVNTVKRDPAYV
                     NLSEEQIESLYNGTWIIATGIAALMSSSIIHPTDEQIEKMIQDSIDAILKFKEPIEID
                     "
     gene            complement(489..929)
                     /locus_tag="ACF3T5_001761"
     CDS             complement(489..929)
                     /locus_tag="ACF3T5_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFD3632362.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            1087..1851
                     /gene="map"
                     /locus_tag="ACF3T5_001762"
     CDS             1087..1851
                     /gene="map"
                     /locus_tag="ACF3T5_001762"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFD3632363.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            1883..2806
                     /locus_tag="ACF3T5_001763"
     CDS             1883..2806
                     /locus_tag="ACF3T5_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFD3632364.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            2882..4021
                     /locus_tag="ACF3T5_001764"
     CDS             2882..4021
                     /locus_tag="ACF3T5_001764"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFD3632365.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            4014..4814
                     /locus_tag="ACF3T5_001765"
     CDS             4014..4814
                     /locus_tag="ACF3T5_001765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3632366.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            4814..5641
                     /locus_tag="ACF3T5_001766"
     CDS             4814..5641
                     /locus_tag="ACF3T5_001766"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3632367.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            5646..6353
                     /locus_tag="ACF3T5_001767"
     CDS             5646..6353
                     /locus_tag="ACF3T5_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD3632368.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            6453..7319
                     /gene="rfbA"
                     /locus_tag="ACF3T5_001768"
     CDS             6453..7319
                     /gene="rfbA"
                     /locus_tag="ACF3T5_001768"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFD3632369.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            7333..7905
                     /gene="rfbC"
                     /locus_tag="ACF3T5_001769"
     CDS             7333..7905
                     /gene="rfbC"
                     /locus_tag="ACF3T5_001769"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFD3632370.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            7927..8955
                     /gene="rfbB"
                     /locus_tag="ACF3T5_001770"
     CDS             7927..8955
                     /gene="rfbB"
                     /locus_tag="ACF3T5_001770"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFD3632371.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            9053..9904
                     /gene="rfbD"
                     /locus_tag="ACF3T5_001771"
     CDS             9053..9904
                     /gene="rfbD"
                     /locus_tag="ACF3T5_001771"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFD3632372.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            9938..11971
                     /locus_tag="ACF3T5_001772"
     CDS             9938..11971
                     /locus_tag="ACF3T5_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632373.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            12015..13295
                     /locus_tag="ACF3T5_001773"
     CDS             12015..13295
                     /locus_tag="ACF3T5_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFD3632374.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVCTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         4083 a         2251 c         2821 g         4150 t
ORIGIN      
        1 atatccattt tgcaagaaga gagaagaagt tgtatcgttc actgtactta gaagaatatg
       61 gtggaggaaa agagatgcag aatttctctt atacttactt tgtgaataca gtgaaacgcg
      121 atccagctta tgtgaatctt tcagaagagc agatcgaatc tttgtataat gggacatgga
      181 tcatagctac tgggatcgca gctttgatgt cttcaagcat catccatcca actgatgaac
      241 agattgaaaa gatgatacaa gacagtatag atgctatctt gaaattcaaa gaaccaatag
      301 aaatcgattg aatatactca aaagttccac aaatcagtag ataacaagag atgtgtcaaa
      361 ataaggaaaa gttcgttttt cttacagcgt atgcattttt aactcccgaa gaactcttcc
      421 atgaatgcaa tgaataaaag agccgtacag attgaataaa tctggatggc tcttttattg
      481 caagtatatt aaatttttgc gactagttct ttagcgaaag cttctaaatt ttcgatatct
      541 tcttcttcag cattcagatc cactttaaca ttttcggctc cttttgatgc gcctgttttt
      601 gcaaaagcag catcgaaatc atccaccgat ttgcagaatt catcgtaaaa agtatcacca
      661 gatccgcaga caccgtaaat ttttcccgat agatccaatt cttgcaagtc ttcataaaag
      721 tctacgatct catcaggcag atcgccatca tcgtatgtat aagtagctac tacgcaaata
      781 tctgcctcct cgaattcatc tgcatctact tgggtacatt cgttgatttc gacttcgata
      841 tccagatttt caaatgcttc tgcaacaata tcggcgattt cttcagtgtt tcctgtcatg
      901 ctagcatata cgatttttgc taaagccatg atgtgcctcc tcgtttataa ttatttaaca
      961 attatgaatt ataccaaaac atcattgaaa agaaaataaa acctagagac acctggggaa
     1021 tgtcatttgg ctaaatgtat gccgctatgt taaaatgaga gggatttgta aaaaggagac
     1081 gaaaatatga ttactttaaa atcagcacgt gaaattgaaa tgatggcaga atctggagct
     1141 ttattagcag atgtacataa aaatctgcgt gattttataa aaccaggaat cacaagttgg
     1201 gatattgagg tatttgtcag aaactatatc gagagtcatg gcggaattgc tgcgcaaatt
     1261 ggctttgaag ggtatgaata tgcgacttgt atcagtataa atgatgaaat ttgtcatgga
     1321 ttccctcgta aaaagccttt gaaaaacggt gatttggtaa aagtcgatat gtgtatcgat
     1381 ttgaaaggcg gcatttctga ttcttgttgg gcctatgtag ttggagaatc tacacctgag
     1441 atcgaccgat tgatggaagt aacgaagaaa gctttgtaca tcggtattga gcaagcacaa
     1501 gtagggaatc ggattggtga catcggtcat gctatccaaa cttatgtaga aggcgaaaat
     1561 ctagcgatcg ttcgtgattt tattggtcat ggcgttggtc ctacgattca tgaagaacca
     1621 gcagttcctc attacggaga agctggaaaa gggttgcggt tgaaagaagg catggttatc
     1681 acgatcgaac ctatggttaa taccggaact tggaaaatga aaatggatcc taacggctgg
     1741 actgcgtata caagagacgg cggactttct tgccaatatg aacatacatt ggctattact
     1801 aaagatggtc caagaatttt gacatcacaa ggagaagaag gcacttattg atctatccag
     1861 tcaataggta cgggagaggt acatgaaagt ttggaacaaa ataaaacaaa atgaaaatct
     1921 gatgcgcttt attgagacga ctcaaaagcg catcactgat tcagaaatga gtacgacctc
     1981 tgtcgtcgtt gcttattatt tactcttgtc tttatttcca ttgattattg cttttggtaa
     2041 tattttaccg tttctccaca tcgatcaaga gactgtactg acctatttac gagaagtgat
     2101 tcctgagacc gtttaccagt ttatcggtcc tgcaatcaaa gaccttttga cacaaagttc
     2161 aggaggcttg ttatccgttt ctgcattagc ggctttatgg tctgcaagtc aaagtatcaa
     2221 tgctttgcaa attgcgatga ataaagcgta tggcgtagaa aatagaaaaa attttttgat
     2281 cgtccgtttc ttctcactaa ttgtcattgt cttgttcatg attgctatta gtggggtaac
     2341 tatcgttctt gggttgggac agctgatttt ggatgctatt cagccaatcg tacaaatacc
     2401 cgtgaacttt atcgatcagt tccaaacttt gaagtggcca atcacattgg tagctttatt
     2461 tgtcatcatg ttcttgattt atttaattgt ccctaatgct cagttgaagt tgaagacgat
     2521 cattccgggc acgatcttcg ctactgtcgg ctggatgctg ttatcacaag tgtttagtat
     2581 ctatgctagg tattttgcaa cgaggatcag cggttatcag atcataggaa gttttattgt
     2641 actcatgctt tggcttaatt ttgctgctac gatcatcatt ttgggcggaa tcatcaatgc
     2701 agttgttcaa gaatacgtga cgggtcatca aatcaaaaaa cgtcgagatc caagccgaaa
     2761 atggttcaat aaattgaaaa ctaaattttc tcataaaaaa aagtagaaac aagagttctt
     2821 attgtaaatt tatatcagta atttataata attatgactc ttaattaaag taggtgccca
     2881 tatgcttgtt tcttttagta tggttataag attttttctg acaattttat tatcactgat
     2941 attaacacct gtattcaaaa tcatttcagt acaaacaggt atggtcgaca aaccaaatga
     3001 aagaagaata aataaagttc caatgccttc agcgggcgga ctgcctatct ttatttcatt
     3061 tgttatatcg actttattct tattccgtaa aattatccct caatcgtata ttgtaccgat
     3121 cttgattgct tctttagtaa tcatagtcac aggagtgctc gatgataaat atgagctgac
     3181 acctaaacaa aaaagtatag ggatcttgat tgcttctctt attatttatt ttgtagcaga
     3241 tatacgtatt gattccttca cgcttccttt tattggatat atccaactag gctggcttag
     3301 ttttcccgta acgatttttt ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg
     3361 tttggatggg ctggcggcag ggatttcatt gatcggttta attacgatag gaatcattgg
     3421 atattttttc ctccatgctt ctacagtgta tattccagtt gttattttct gtcttgtagc
     3481 aagtattatc ggtttttttc cttacaattt ttatccggca aagatttatt taggcgatac
     3541 aggagctcta ttcctagggt ttatgatggc cgtcctttca ctacaagggc tgaagaacgt
     3601 gacttttgtt tcatcgattt ctttgctgat cgtcatggga gtgccggtaa cagacacgtt
     3661 ttttgctatc attaggagaa aagcaaaccg tgtttctttt tctacagcag ataaaaaaca
     3721 tcttcatcac cgactaatca gtttaggctt tactcataaa ggtgctgtcc tgacgattta
     3781 tgcgatggct ttgatgtttt ctttcacagc tatggtcatg aattatactg gaagaatcgg
     3841 aacgatcata ttgattattg ccatgttgtt tgctgcaatt ttattatttg aattgattgg
     3901 tctgatcaat gagaaacatc agtttatttt aaaaacattg cgatttttag ggaataaaga
     3961 gtaccgtacg caagtaaaac agaaatattt gaagaaatgg aaccgtcata tagatgaata
     4021 acaaattaaa ccagccgatc cttattagta ttgtgacggc aaacagtgat aaaatttttc
     4081 ggacactaga taattttatt tcaactgtaa aagatgaaac agcagtaaaa atccgaattt
     4141 ttgacaacaa ttcacttccc gagtacaaaa aaagactaac agagtatagg aaatatccct
     4201 acatcgatat ctattttcat gatgaaaatt ccggatttgg ttatggacat aatcacaatc
     4261 tgatagctag cgattctcgc tatggcatta tttgtaaccc agatatttta gtggatgaag
     4321 agacagttac agcattggtc ttgttattga aagaacatcc ggaatgtgcg atgttagcac
     4381 ctaagattct taatgaagat ggaacaactc agcacctcat ccgtgagcag ctggctgttt
     4441 ttgattacgt tttgcgcttt gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac
     4501 tggctgtgtt tgaatgccga ggattgccta ctgatgctaa tagttatgta cggatgatct
     4561 caggtagttt tatggtagct gatattgata aatttaaaga gataaagggg tttgatgagc
     4621 gtttcttcat gtactttgaa gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca
     4681 agttattgta cacacccttt tatgcagtta tccattttta cgggaagggt gcgcatcgaa
     4741 atgcaaaact gtttcgcgtg tttttgactt ctatgatgaa atttttcaat aagtgggggt
     4801 ggcgtttttt ctgatgaaac cagaaatatt agtagtcgtt gtgctttatc agcaactatt
     4861 cagtcaatct ccagcttatg gaccattaag caaagcactt agcgaaaagc aaattcagct
     4921 tattatttac gataatagtc ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat
     4981 cgtttatcat catgatccgc aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc
     5041 tcaagcagat gaaggaacac gttgtctcgt cactctggat caggatacga ggctagtcga
     5101 cgattatttt gaactattgc gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc
     5161 gatgattttt tctgggagcc gacaagtttc tcccctatat tcagatcgtt atatcaatcg
     5221 tacagctcaa gcagtagaag ttgaaggccc aacagctgaa cgaatcatgg caatcaattc
     5281 aggtgttgcc tggtctgtca aatttctaaa aaagattggc gggtttaatc ctgctttttc
     5341 tttagatttt ttggatcatt ggctgttttg gaaagtggat cagttggaaa aaactgtgga
     5401 gatccttcct gtacgaatgg aacatgatct ttcggtttta gactatgaaa aagtttcaag
     5461 taatcgatac cgttctattt tacaagcaga aaatcgattt tatcatgaat atgatcaaag
     5521 gcatttatcc agtcaccgac gacaattatt gttaagaaca gctaaacagt tcttgaccgt
     5581 caaaaaccgg catatctggc ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg
     5641 aattcgtgcg ttcggtttgt atagctacct ttaatggagg cagatattta agagagcagt
     5701 tagatagtat tttatctcag ctttcagcag aagatgaagt agtcatttct gatgatgggt
     5761 cagtagatga tacatgggat atattaactg aatacgcaga aaaagataag cgaatccagc
     5821 ttttttcggg gcctcgtcaa ggattgatcg ctaattttgg ttatgcgatc cagcagacga
     5881 aaggagatct aatctttttg gcagaccaag atgatgtttg gttggagaca aaagtgaaaa
     5941 agatcactga ctattttgag cagaatccgg atcagctagt agtagtttct gatttaatca
     6001 ttgtggacag cgagctgcaa gagatccatg attcgtattt ttcgtttcga aattccaaag
     6061 aaggatggct taataatgtg ataagaagtc actatattgg tgctggtatg gctttccggt
     6121 cttctttaaa acccgatatt ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga
     6181 ttggtttgct gggaggaaag aagactgggt tcctaagaga accgttaact ctgtaccgaa
     6241 gacatgattt aaacgcgagt gagattgaaa cacattctaa attacggcaa aaaatttctt
     6301 ggcgtgttca tctgattacg gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga
     6361 tgtttaaatc cttaatattt ccatgacaaa tataacaatt gatgagataa taacaaagtg
     6421 aaatttgaaa tcaaaaagga gagcagatat ccatgaaggg aatcattctt gctgggggca
     6481 gcggaacacg tctgtaccct ttaacaaagg caacatcgaa acaattgatg ccgatttatg
     6541 acaaaccaat gatttattat ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc
     6601 tgattatttc aacgccacaa gatacaccac gtttcaagga attatttgga aacggaaatg
     6661 atttaggact gcagatcgaa tatgcagtac aagaaagtcc agatggattg gcacaagcgt
     6721 ttatcattgg agaagaattt attggagatg atagtgtctg cttggtatta ggggacaata
     6781 tttactatgg tggcggtctg tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg
     6841 cgacagtatt tggctatcat gtcaatgatc cagaacgctt tggtgtagta gaatttgatg
     6901 agaatatgca agctttgtcg attgaagaaa agcctgctca gccaaaatca aattacgctg
     6961 taactggcct ttatttttat gacaatgagg taatttctat cgctaaaggt atcaaacctt
     7021 cagaacgtgg agaactagag attacggatg tcaataaagc atatttggat aaaggaaaac
     7081 tatccgtaga acttatggga cgcggatttg cttggcttga tacaggaaca catgaatcat
     7141 tgcttgaagc atctaccttt atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt
     7201 gtttggaaga aattgcttac cgtatgggct acatcgataa ggaacaattg ctggcccttg
     7261 cacagccatt gaaaaaaaat caatacggtc aatatttatt gaatttggcc gctgaataag
     7321 gagtccttac agatgaaagt aacagataca aaattaacag atgtcaaaat cgtagaaatg
     7381 gacgtctttg gcgatcatcg cggtttcttc acagaaagtt attcaaaagc aaaatttgca
     7441 gaacatggat tgaaatatga ctttgttcaa gacaaccatt cactatcagc tgaagctggt
     7501 gtccttcggg ggcttcattt ccaaaaagga gaagctgcac agacaaaatt gattcgggta
     7561 gtcactggag cagttttaga tgtagtagtg gatatccgca aaggtagccc gacttatggt
     7621 caatgggacg gatttatctt atctgaacat aatcatcgac agttgcttgt acctaaagga
     7681 tatgcccatg gattcgttac tttgacacca aatgtgaact tcctttataa gtgtgataat
     7741 tactataatg cagaagcaga tgggggcatc gcatttgatg atccagattt agcgattgat
     7801 tggccaattg atccatcaag agcgattatg tcggaaaaag atcaccgtca tccgacatta
     7861 aaagaatttg aagcagaaaa tccatttgtt tacggagaaa tctaagaatt aaggagaaat
     7921 tttcttatga aaaacatcat tgttaccgga ggtgccggat tcatcggctc taatttcgtt
     7981 cattacgtgg taaacaatca tccagaagtg catgtcactg ttttggataa actgacttat
     8041 gcaggaaaca aagagaactt ggctggactt cctagcgatc gcgtagaatt ggtcgtagga
     8101 gatgttgcag atgcagaatt agtggaccgt ctagtaaaag aagcggacgc agttgttcat
     8161 tacgcggcag aatcccataa cgataattca ttaaaagatc ctttcccatt tgtacaaacg
     8221 aatatcattg gtacgtatac acttttagaa gcttgccgca agtatgatgt acgctatcat
     8281 cacgtatcta ctgacgaggt ttacggcgat cttccattac gtgaagacct gccaggacat
     8341 ggggaaggcg aaggagaaaa attcactgcg gaaacaccat ataatccttc aagtccatat
     8401 tcttctacaa aagctggttc agatctgtta gtaaaagcat gggtgcgttc ttttggctta
     8461 cgtgcaacca tttcaaactg ttcaaacaac tatggtcctt atcagcatat tgaaaaattt
     8521 attcctcgcc aagtaacgaa tatcttaagc ggtattcgtc caaaattata tggcgaagga
     8581 aagaatgttc gtgactggat ccatacgaat gatcattctt cagcagtgtg gctgattttg
     8641 acgaaaggca gaattggcga aacctatctg atcggtgcag acggtgaaga agataataag
     8701 accgtgatgg aattgatctt agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta
     8761 aacgaccgtg caggacacga cttgcgttat gcaatcgatt cgacaaaatt acgggaagag
     8821 ttaggatgga agcctgagtt tacgaacttc cgtgatgggt tggctgacac gatcaaatgg
     8881 tacgaagaac atgaagactg gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc
     8941 aaaaatggac aataatacag agaaaatgtc actgtattaa tggaaaaaca catgtgaggc
     9001 aggcgctcgc ctgtctcttt tttaaaataa aaatcaagga gcggataatc atatgttttt
     9061 attaactgga ggaaatgggc aactaggtac agaattgcgc catttattag atgaaaaaaa
     9121 tctgcaatat gtttcaacag atgcaaaaga cttggatatc acagatgccg agaaaacaat
     9181 ggcttacatc acagaattaa aacctgatgt tattttccat tgtgcagctt acacagcagt
     9241 agataaagca gaagaagaag caaaagaatt agatgagaag atcaatgttg atggtacaag
     9301 aaatgtagca cttgcagcaa aagctgtgaa tgctcaattc gtctatatca gtacagacta
     9361 tgttttcgat ggaaaaaaca aaaatgtaga atacgaagta gacgaccaaa cgaatccact
     9421 aaatgaatat ggtcgtacga aattattagg cgaaaaagca gtagaagaga ttttagaaga
     9481 ctattatatc atacgaactt cttgggtttt cgggatctat ggacacaatt tcgtcttcac
     9541 gatgcaacgc ctagcggaaa cccgcgacaa actgactgta gtaaatgacc agtttgggcg
     9601 accaacttgg acacgtactt tggcagaatt tatggtatat gtcatcgaag aaaaagcacc
     9661 atttggtatt tatcatttgt cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga
     9721 aatattgaaa gatacagatg tagaagttgc acctgttacg tcagaagaat ttccgcaaaa
     9781 agcaacacgt ccgcagtatt cagtcatgag cctgaaaaag acagaagagt taggatttgt
     9841 cataccaact tggcaagaag cacttgcgca aatgctggaa gctgccaaaa aaatgaataa
     9901 gtaatttcct tttatcaaat agaatttgga gcatgatatg gaaaaacaaa caagaagtgc
     9961 acgacgaaaa gaaaagccaa aagcgaacca ggcagattcg aatttggtgc ttgacctgtt
    10021 tctaaaactc cgctatctta ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt
    10081 aaacggaagt tcacttggtg tttgggataa atatgtgtcg caaagagacg atgggaaaaa
    10141 aactgatgtg atttttggac agaatcggga agtgcggtca gacgaatggc tggtgcagac
    10201 acctttttac ctctcacaag ctgaagagga ctttccgttg gtgaacgagg attattcatt
    10261 aaacggacaa aacatgatca ttgcgtataa ttctcctgca aaggatatta cggttatcgg
    10321 aaaaccattc aactggggat tctttttcct cggaagagat cgaggacttt ccttttattg
    10381 ggcgatgaaa ttagtcgtca tggtcttgct tggattcgaa gtactgatga tattaacgaa
    10441 gagaaaaaaa gctctatcgc taatcggtgc atttgcgctg acattttctc cagcagtcca
    10501 gtggtggttc atgcaacatg tgggcgattt gattttcttt actttgggac tgatgactgc
    10561 tttttatcat tatttctaca atcatgataa aaaatggcta cgaacgttga tgatgctttt
    10621 ggtggttatt ttcggtattg ggtttatttt agtgatttac ccagcccatc aagtgatgct
    10681 ggcgtacttg ctgatttttt attttgttgg cctgcttatt tactatagca agaaagtttc
    10741 atgggatctg ttggatgtag tgttgattgg aggggcagtt ttatttattg gcggagtcat
    10801 ggttcatttc tggctaacgt caaaagacgc attgttagct tctttgaata ccttatatcc
    10861 aggaaaacgg gtaagtactg gaggaaagtg gacgattggc gaattttttt ctttccttac
    10921 aaattggaaa attccttttg aagatatcaa ctattcgaat aactctgaag tggccctgtt
    10981 ctatcacttt tttccaactg tattcctagc aagtccattt gtgttatttg gcaaaaaaga
    11041 tagcgaacaa aagttactgg gacgtatttt gatgattttt tgtctgtttt ctatcttttg
    11101 gattaccgtt gggttgccaa aatggctagc agaaatcact ctgctgtctt atgttcctcc
    11161 attaagagcc atccttactt ttagttttgc ggcttgttta ttatcgatct ggtttatcgc
    11221 ttatatttgg agagaagatg ttattcctat ttggtataaa ttcattttgt tggcagtaaa
    11281 tgcatggtct tatttctatg cgattacgca ttcgaaaata gataattatt tttcggattt
    11341 tgaaaccatc gcagttgctg tgttgggaac agtgatttta gcatttgcat tatttggctg
    11401 gagacgcttg ttttacctcg cttttgccgc cttagttatc gtatctggct ttttcgtcaa
    11461 tccagtcgta gaaggtacag gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa
    11521 aatcgatcaa caagatcctg gtaaagtttg gttgtctgaa gatgaattat ataacttcac
    11581 cccaacattg ggagtgaaat ccttcaatac agtacgcttt tatccagata tagctgcatg
    11641 gaaggaaatc gatccgaaag aagaatacga gaagtattat aatcggtatg ctcatacaag
    11701 agcttttatc gctaaggaag atacaaagtt cattttagat cgtccagata tgttttcggt
    11761 cacgttgaat tttgaagacg cagaaaaatt agggatcgac tatgtgatca gtaaacgacc
    11821 attaactgat tacaaccaat tatatgaagc gcagttcacg cagctatatg gaccggataa
    11881 agacggttat atgattttta aagtagaata tccggattat ccagatgtac cacaaacaca
    11941 aactcaggaa aatcagctag tcgtacagta ataggctagc tgagaataat cctgaaatat
    12001 tagtagaaag gagagtggag ataattcaag caatcgtaca aaaatttgtc aacaaaatga
    12061 gtgtcggttt cttgcttttg ttgctgacgc tagtcttttt tgtttgtaca gcagggttag
    12121 gttttccagc aactttgttt atatataaca atagttcatt acttatcgct tttagtgtgg
    12181 ctattttgct cattttaaat gtttataaag caaaaggcgt cgattttctt tatctaggtg
    12241 ttgcgcttgc ccttttcatt ttcttttctt actttcggga attaagaaat gagtctttat
    12301 ttggagattt actgtttcct ttgattattt tattttgttt agtaattaaa tggatcgagt
    12361 ggacaaaatg ggatcgcggt atttatctga ttgtctttac ggtcttcctc ggaatcacgt
    12421 tatatcgtgt atttacagag atccaagtac cagaaggaca aagcatctgg gctccaggca
    12481 attcgctttc tgatatttgg atcaatacga atacaatcgg aagttctttg atgactcttg
    12541 ctttattgat cagtggattt gccagttctt ttgaacgatg gtatgtacga atcatcggtg
    12601 cagtagcagt tgttgcagct tttctcggga tttgggtctg ccaatctaga ggtgcattag
    12661 tcgcgttgat tgtttttgcg atattggatc ttttacctaa atcgttgatg cgtgtcattc
    12721 gtgcgccatt tattgcttac acggttacga ttcttttagc tttgccgatt tcttatctgg
    12781 cggctgtctc tgaaaaagtc aatttgttta ccggtcgaga agatatttgg cataaattct
    12841 atcagacgct aggagaaaaa tctgaacaag ttttactagg aatgaaaaca tttatcttcc
    12901 aacgaggaaa ccaattttta ggaaaccaca atagttataa ttctattttg aatatctatg
    12961 gattgatagg atttgggatt gctactttgc tgcttatcct ttttattggt cgcttaacgt
    13021 tgaaagcaga tttatccaac gggcagatga cttttatctg ggcctttttt gcggtgatga
    13081 tgcagtcatt tatggaggat acattgactt catacccatg ggttccgatt atttatatcc
    13141 tgttggctat ggccgttcat cgatatgatg aaccagaagt tgcggaggaa ttctatgtta
    13201 tggaagaggc atatgacgat atgacttatg atgatacatc ttatgaggaa caaccagata
    13261 gcgctcattc ttccaggatc caacgctatc attgataggc acaaa
//
LOCUS       DAXLPI010000069        13222 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXLPI010000069 DAXLPI010000000
VERSION     DAXLPI010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13222
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.069"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            158..1027
                     /locus_tag="ACF3T5_001774"
     CDS             158..1027
                     /locus_tag="ACF3T5_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD3632375.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1236..2261
                     /locus_tag="ACF3T5_001775"
     CDS             1236..2261
                     /locus_tag="ACF3T5_001775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFD3632376.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2300..3250
                     /gene="manA"
                     /locus_tag="ACF3T5_001776"
     CDS             2300..3250
                     /gene="manA"
                     /locus_tag="ACF3T5_001776"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFD3632377.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3344..5047
                     /locus_tag="ACF3T5_001777"
     CDS             3344..5047
                     /locus_tag="ACF3T5_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFD3632378.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5049..7082
                     /locus_tag="ACF3T5_001778"
     CDS             5049..7082
                     /locus_tag="ACF3T5_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFD3632379.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7093..7533
                     /locus_tag="ACF3T5_001779"
     CDS             7093..7533
                     /locus_tag="ACF3T5_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632380.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7733..8860)
                     /locus_tag="ACF3T5_001780"
     CDS             complement(7733..8860)
                     /locus_tag="ACF3T5_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632381.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8863..9540)
                     /locus_tag="ACF3T5_001781"
     CDS             complement(8863..9540)
                     /locus_tag="ACF3T5_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFD3632382.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9804..11570
                     /locus_tag="ACF3T5_001782"
     CDS             9804..11570
                     /locus_tag="ACF3T5_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFD3632383.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11585..12022
                     /locus_tag="ACF3T5_001783"
     CDS             11585..12022
                     /locus_tag="ACF3T5_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632384.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12035..13186
                     /locus_tag="ACF3T5_001784"
     CDS             12035..13186
                     /locus_tag="ACF3T5_001784"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFD3632385.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4491 a         2298 c         2731 g         3702 t
ORIGIN      
        1 cacaacctct ttttataaga gtttttactc attaatttac aaaaaacaag caaaatgcga
       61 attttctcac atttttttta acaaaatcaa agttattgct tgaaagaaga agaatccatc
      121 gttaaaataa agcttgacgt agaggggagc aagaaatatg aaagattttc aattgggggc
      181 tgccacctat ttaaaaagtg tagccattcg ggtgaaagac cgagataaaa tgattgattt
      241 ttacaagaac gttgttggct ttgatttgaa aagagaagaa aacgaattag ccattttagg
      301 aacagaagaa gctggaagtg aaatgttgct gctggaagaa agtcctagag cgaatgatca
      361 catgggggtg atcaaaaagc tgaaccgttt ttcattgatt atccctagca cagaagaaat
      421 ggcagatatc ctttgtcgta tccgtaaaat gaattatcca atcaaagaag ctttagaaga
      481 tcatggacgt atggggattt tgctcgtaga cccagaagag aatgaattgg aaatttacca
      541 tgaagagaat aagaacgacc aaagagaaat tccagaaaca atggatcaag aagcattatt
      601 aagaaaatct gaagaaaaat tcagtaaact atcaacaggt tcttattttg aaaaaatcca
      661 tttaaatgtt actaacttaa caaaagaacg ccaatttctt aatcatgtac ttggtttgaa
      721 ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagatttcc atgttggttt
      781 gacagaagct agcggaggcg caattgattt gcctacagat gaagtgattg gacttgattt
      841 tctgaaattc atgatcgata ctgaaagcat ggaaggactg attgctcact tgcagcaact
      901 atcagttgat ttcttcttag acaaaaagca cacagtactc acaatctatg atgctatagg
      961 aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca ttcgatgcga
     1021 cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg aacggtcata
     1081 ctatcgctta ttctagagat agaaagagaa aatagtatac cgggggaaac tgcgcggttt
     1141 ttcgcgcagt ttttttgtgt aaaaaaggtt gattcctcat agattaagct ttaagcttgg
     1201 actgtaagaa tctgatagaa aagcaggaga aaaagatgta tccacacacg aaacagctta
     1261 ttttgaaaaa gatggaagaa ggattttatc caggtgtagc agtcgccttc ataaaagaaa
     1321 atcaagtaga aacatttaca gccggctttg cccaagtcgt tcctgaaaag cagctgatga
     1381 cagaagatct gttgtttgat gcagcctcac tgacgaaagt cgttgcaacc acttcattag
     1441 ttttacgtct attagaagat ggaaagatag aactcgatca accgcttcat aaatatcttc
     1501 ctgaatttga aaatcagaca atcactttgc gtcatttact gacacatact tctgatatca
     1561 aaacatggat accaaacaga gataagctat ctcaggaaga attgatcgcc gcttatttga
     1621 agctagaacc aggtgagcaa ctaggaaaag aagtaaaata tacagatgcc ggtacgattc
     1681 ttcttgggtt gatgctagag gaaatttacc aagaaactgc agtggaaatc tttcaagaac
     1741 aagtattgga accattagga atgatgaaca gcttatttct tccttttcct tccaaaaaaa
     1801 tcgttcccac agaacaactg ccttcgggtg aggttttgcg tggacaaact catgatccga
     1861 aagcaagagt tttggctgaa catgcaggta acgcgggtct ctttattgat ttgaaagaca
     1921 gcattacttt tgtccaaatg tatttgaaca agggaaaaat ccgtaatggt cgttttttag
     1981 aagaagagac gattgattct ttactgcaag atcaaacacc gaaaaaaaat ggctatcgtt
     2041 ctttaggatg ggacttaaaa aaggatgtag aagacggtca tgcgattctt tttcatactg
     2101 gttatacagg tacgtttttc ttgattgata taaaaacaca agaagcgttg atatttttgt
     2161 caaatcgcgt acatcctgtc gatcatcgtg cggagtatct catatgtcga gatgaaattg
     2221 ttgaaaactt tttgaaagag aaagcggtat taccgaaata atgctataat agctatgaaa
     2281 aatgagagga gtttgaatta tgcaggaacc tatgttttta aaaccagttt ttcaagaaaa
     2341 aatttgggga ggaagtcgtt tacgttcagt ctttggtttt gatatcccta acgataaaat
     2401 cggagaagat tgggcgatca gtgcgcatcc tcatggtgtc agtgtagtag aaaatggcga
     2461 attcaaaggg aaaagattag atgaactatg gtcagaacac aaggaattgt ttggccatcc
     2521 ttcagaacca gtgtttccat tattgatcaa gatcttagac gcagaagatg atctatctgt
     2581 acaagtacat ccagatgatg cctatgggat gaaacatgaa ggtgaattag gaaaaactga
     2641 atgctggtat atcatcgatg ctgaacctgg tgcagaaatc atctatggtc atcatgcaaa
     2701 gacaagagaa gaactagctg agatgatcaa agatgggcgc tgggatgacc ttttgaagaa
     2761 agtaccagtt aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc atgctattgg
     2821 taaaggaatc atgattttag aaacacagca aagttctgac acgacttacc gcgtgtatga
     2881 ctacgacaga aaagatgcgc aaggacaaac aagagagctg catatccaac aatcgatcga
     2941 tgtaacaact gtccctgcga aaacaccaga attacaaatc aaagaagtcc gaaaagggaa
     3001 ttcatcgatc gtcacttatt tagaaactga gtttttcaat gtatatgagt gggatattaa
     3061 aggaatcaca tctttcaaaa aacaagcccc ttatacattg atgacggtaa tcgaaggagc
     3121 aggagacctt gtcgttgacg gaaaaacata tccattagaa aaaggaacaa gctgtattat
     3181 tccaaatggc gtttctgagt gggcggtcca aggtgaacta gcaatcattg cgactgaacc
     3241 aggcaaatag ttcccagata gctgaaagca gcagacggtt tttcgtttgc tgctttttct
     3301 acataaaatc cgtccatcaa taaatagaag tatggtgaaa aagatggaat acaaatggaa
     3361 agcaaacaac agcagtcgcg tgtctatgaa acaactgaga aaagctggac atcacttaaa
     3421 tgaaatgatc atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt cattagcaac
     3481 aaaaggtgtg tctggcgatt tgcacaccac attagaagag gtatgccatt ggatgatcca
     3541 gctgctttta cccatcttga tcagttattc aggcgggaaa tcggtagggg gacaaaaagg
     3601 ggcagtcgcc ggtgcaattg cttctttagg gttgatcgct cgttccaatg ctccacaaat
     3661 cgtaggagcg atgattatcg ggccgcttgc cggcatcgta tatgaacaat ttgtttctag
     3721 attccacgtg aaattcaagg ctggatatga aatgcttgtc agcaacctac tagtaggact
     3781 gactggtagc gttatttgta taattggaat cgtttttatt gaaccagttt taaatagtgt
     3841 tcacttgttg ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt taccacttgt
     3901 cagtctgatc ttagaacctc taaaagtact atttttcaat aatgcaatca atcatggtgt
     3961 attgacacct ttaggaatcg aattctcaca aacagtcggg tactcttcct tatttttgat
     4021 ggaggcaaat cctggacctg gtttagggat cttactttct atcctttgtt ttgcagaaaa
     4081 acaggaaaaa gtaaatgcga gtggagcatt gatgatccat gcgattggcg ggattcacga
     4141 aatctatttt ccatttgtct tgctgcgacc gtatctattt ttagctgtaa tggctggtgg
     4201 ggcaagcggc acgttgatat ttcaatggtt agatacggga ttagctgcac ctgtatcgcc
     4261 tgggtcacta ctcgtgattt ttgccaatgc gccttatcca caactagctg gggtaagtat
     4321 agggatagca atcagtacat ttgtgacctt catttgtgca gcgatactat tgaaattacc
     4381 aaataaagaa aaagagaaca aaacggtcaa agaaagggga gaaatcttgg aaccatctat
     4441 cattcattcc gtcatttttg cttgcgactc aggtatggga tcaagtgcta tgggagccgc
     4501 cctgctgaaa aagaaattag cagcagaaaa aatcgatctt tctgtcggat acagctctat
     4561 ctatgaattg aataaccaag taaatcagct ggttatcgtg caacatgaac tagcgggtct
     4621 tgcaagagaa aaagtacctc aaagtcaaat ctatgtactg gatcactttt tggaaacaga
     4681 tatcttgctg tcacgaataa aagaagcgat ccaatttcca aagcgggaag aaaaaaaaga
     4741 gatagcagat atcacagaac aaaaaaagtc gcctgctaat attgtatttt tgtataaaaa
     4801 caacagacgc ggctcacaaa ctatgggcat gacgatttta agtcaattag caaaaaaagc
     4861 gaaaaaacag tttgttatct caaaagaatc aatagaaaaa atcaaaatcc aagaagaaac
     4921 gatttatatc gcacaaagag aactagctgt aactgaacag ctagaagaaa agatccctcg
     4981 gctagttcta gttgaacatt gggtagctac gaatgaatac gaaaaactcg tgaagggaga
     5041 aatataagat gtatttatcg cccagagaaa aacaattatt agcagaattt ctcagtagcc
     5101 cgtcacctgt atctatccaa aaaatgatga atttattgaa ggtcagtaaa cggactattt
     5161 accgcgaatt agataatctt gaagtctcat tgaaatcggt aaatgcttct ttgaaaaaag
     5221 cagctagagg ctcatttgtc attgaggcat cagaagaagt attagagcaa ttaaaagaaa
     5281 atacagatgg gaaaagtaat gacttatcca ctgctaaacg acaacacgcg attttagccg
     5341 agttgttact gacgacagag ccgttgagtc ttgctcattt catggaatca tatcgcatca
     5401 gtaataccac tttttatgca gatatcaaac agttggaaga aagtattcgc caactgcctt
     5461 tggaaatcat tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac cgccggcttc
     5521 tgacagcgaa tgtattagaa ttagaaatca atgaatatga actttttcat agtatttctt
     5581 tcgatgcatc actgaattat ttttttcgat tcgttgatcc gcaacatcta tctcttgcac
     5641 gcaaagtaat cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat agaaagcttg
     5701 aacatttggt tttgatgctg acattgacga tggagcgtgt gactaaaaat catttactga
     5761 caaatgagac atataatagt ctggcaaaca aagaattact acagacagct aaacgattgt
     5821 tttcacggat cgcttctgag acaaaacaac tttatccagt aaatgagatc gtcttttttg
     5881 caggattatt aaatgacttc acgaactcat ttgatcagaa tttctttgaa gagacgttcg
     5941 atacacagct tgcttattta gtaaaacaac tgattcaaga agtaagtgaa gagacgaatg
     6001 tccattttta tgaagatgaa actttgtaca aaatgctgtt gactcattta tctggtgtgt
     6061 tttcaagagc cgttttacaa gaagagacac tgaccaatcc gattttagaa aaaatcatgg
     6121 ttcaatacga ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt tttcaagaaa
     6181 aaaaattatc cgaagaagag atcgcttata tggtccttca ttttgcgaat tcattggaac
     6241 gaagtccgaa agaaagcaat atcagtattg caggattttc gccaagtgga ttagcttcta
     6301 ctagcatgtt ggaactacga ttgaaaaggt attttccctt catccaacaa attcatttct
     6361 atcggattgc tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt gttatttcta
     6421 catctatttt accaggatac ggtggaaaat atattttagt ctccccgcta ctgctagaag
     6481 atgaagtcaa gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa aggcaaaaaa
     6541 ttacgtataa cagcaataac gagtggatga tagaagatac ttatgaggaa acaatgcatt
     6601 tcatggatca aatcaatcaa ttactcggtc gtttttttat ccaagaactg gataatcctg
     6661 agacgatttc cgaaacgatt cgcagcttat ttccttattt tcctgaagga ctagtaacgg
     6721 atgaagaggt ggttcatcgc cggctgctta agcgatatga acaagcacct atcggtattc
     6781 ctcatacaaa tatggggtta ttccatacat ccagctcatt gatttctaaa ccgatctttt
     6841 gtattttcaa tttgaaacat ccgttgacca ttgaagggat ggatagacag ccagtcgatt
     6901 tgtcccgtat gttagttatg ctggcgcctt cgccaatcca agaaaaagac gggattcttt
     6961 taggaaagat cagtggagca atcatcatga atgacttgaa caccgagatc ttccgctcag
     7021 gaaataaaga aatcgtttat cagttgttag cgaaaatcct gattgaagaa ataaagaaat
     7081 gaaggtgaaa agatgataca gctaccagca gaaaaaatat tgctcgatca atcctatcaa
     7141 aattggaaag aagcgttgac cgagatcggc cagtggatga cccaacaagg gggaatcaaa
     7201 ccggcatata tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta tatcgggaat
     7261 ttcgtagcct tgccacatgg gactgacaaa gatgaagaag tagtagaaga aggaatctat
     7321 ttagtccaag tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa aatcgcgaca
     7381 ctcttatttg tagtggcttt aaaaaaagaa catcagttag cggtgctcca agatttagcc
     7441 tttttctgtt cagacatcga ccaagtaatg gcgctcagtg atgcgaaaac cattgaagaa
     7501 gtccaaatga tttttgaaca tggacatata taaccaatgt tcacagtaaa aaaagctgcc
     7561 ttgcatcaag taataacttc aaccaagata aaaataagag gagctatttt tgttgggagg
     7621 ttcttattac tgcggatgca agcttttgaa aactatttat tctagaaaac cgtttatttg
     7681 aaatagggga cggtgacaag gtttttgtca ccgcccctat tatttttgtg ttttaatagc
     7741 tcttttttat atagatagtg cccataccta tgaataataa aacgatactc catataccaa
     7801 gtgtcaagat accattcatg aaagtaacct gttctgaagc tagtggcgag aaaatatctc
     7861 ctcctatgat cacattttga aaatcaacat aagaagttgg caagtaatga ctgattgatt
     7921 ctatccatcc aatcgaagaa aacaagcgat actctgctaa gaaaagacaa agaagtgtac
     7981 ctaaaagatt gactagagaa tgttcaaata aagcagatag aagaaaactg attgtactta
     8041 aaaagagcac ccacaacaac aggaacagaa agctctggaa aagatactca tacatcggaa
     8101 ggattcgtat ctcctgacca atgatcgttc ctacaggata acggaagtct cccaaaccat
     8161 ttttgattat tactagtata gagacgatca aaaatggcat cacgagactt agcatagctg
     8221 caccataata aatggtaaaa cggatgctat gtttctttac catactatcc ggtaatagat
     8281 tgactagaga gattacctgc tttctttttt cataagagat gatggaagaa agaaacaagg
     8341 cggttataca taaaatcaaa aaactagaaa tcattcctga aaaaatattt tcgtagtaat
     8401 ttgctaaagg gagatgcgct ggcaagtcaa aaatagaata tctttggatc tgatgttctt
     8461 tcatataacg gagcaactcg acacgttttt gttgttcaat gattggaatt ccttgtcttt
     8521 gaccagaaat cagtcgatcc agtatatctt tttcataatg ataagttgct tcgaccattt
     8581 gctggacatt ccctgtgttt tttgcatgta agattgcttc gatcagtttg ttgcttttct
     8641 ctaaactttc cacaaattct ttctcattct catcttcttt caacgtttca atcaaacgtt
     8701 gcatctcttc atagttatga ttcagttcat cgaattctgt ataagcaagt ttatgatcaa
     8761 aaaaaagtgg gaatgcaaag agcacgacca acaatagagg aaataaaaag tttactttgt
     8821 tttttagtaa gagattgcgt tcaaatgtaa gtaaagacat agttacacct catataattt
     8881 acgataaata gctaaaatct cttccatatt tgaggattct gtgacgattt ctccattttt
     8941 tagaaaacat gctttagaac aaatcgattg gatattcgcg atagcgtggg aagaaagaag
     9001 aatcgtcttc ccctctttct gccattgtct gatgaggcta ttcaagatat caatacttcc
     9061 tgggtctaat ccgttcaacg gttcatcgaa taacatgaca gatgaatcac tgatcaagta
     9121 agcagcaagc aagacatgtt gtttcattcc tagagacatt tttccaatcg gcaaagtaag
     9181 gtactcagtc atcttcaatc gttggaaaac agtttggcat tctactttgc tctgccaata
     9241 gtatttgatc aaagtcaaat aatcaacagc cgatagatag tcggataact gattcgttga
     9301 ttctagaaaa aagaaagacc ggcgataatc tgtataagca gtaggagaga tgttgtgaag
     9361 ggacaattga tcataagttg ttggcagcag ttgggcaatg ccattcagca aagttgtttt
     9421 gccaaatccg ttcggggcaa tcaaagcagc tacttctcct gattctacta aaagattcaa
     9481 ttgtcgaatc acacgttttt taccacgttt catgttcatg tttttgatca gtaatgtcat
     9541 agataggcct ccaatataaa atttaataac ataatagaat ggaaaacatc taaattaaat
     9601 gctaacaaaa gttattgttt gcatattata aaaaaattaa aataacagtt ttatttaaaa
     9661 ttgaagtttt ctgataccga aatcttttta ttttgctgac atactcaaac ctttggcaca
     9721 aagtttcgtg ccaaagtacg actatttctc tttttagtaa gcggatacaa ttagagtatc
     9781 aaaaggagag agggagagac aaaatggaac ggacgattcc caaaaacaac atttcgctaa
     9841 aagcgagggt acaaaaatta ggcagtactt tatcaagcat ggtcatgcca aatattggag
     9901 cactgattgc ctggggtgta ttaacggctc tatttatccc agatggttat ttaccaaatg
     9961 aggctttggc taccatggta agcccaatgc taacttatct gattccttta ttgatcggtt
    10021 atactggcgg aaaagtaatt gctggtgacc gcggatcagt tgttggggca attgcaacga
    10081 tgggtgtgat tgtcggaaca gatattccaa tgatgctagg cgcgatgatc atgggacctc
    10141 ttggtggata tgccatcaag aaatttgatc agctattcca aaaacggatc aaatccggtt
    10201 ttgaaatgct agtcaataat ttttcagcag gtcttatcgg ttttggatta gcgttattag
    10261 gtttttctgc gattggtcca gttgtcgatg cattgactca agcaatggca aaaggtgtag
    10321 agatcatttt atctgcccat ttgattccat tgacaagtat ttttattgaa ccagcaaaaa
    10381 tcttattttt gaataatgca atcaatcatg ggattttgac accattggga actgaacaag
    10441 tagcagaggc tggaaaatct attctattct tgcttgaagc aaatcctggt ccaggacttg
    10501 gcgtattact tgcttttact ttgtttggca aaggagcagc aaaatcttca gcaccaggag
    10561 ccatgatcat tcacttttta ggcgggatcc atgaaatcta ttttccttac gttatgatga
    10621 aaccgttatt attcttatca gttataggcg gaggaatgtc aggaagtttc attttccaat
    10681 tgatggatgc tggactaaga gcgccagctt ctccagggtc gatcattgcg attctagcga
    10741 tgacaccgtt caattcttac cttcctgtta ttttgggcgt gattgttgcc gcagctgttt
    10801 cgtttggtat ttctgcagta attttgaaag cggatgcgaa agatacaaat gaagacttcg
    10861 aaaaatctgt tcaagagaca aaggcagcta agcaagcttc caaaggtgcc caaggtattc
    10921 ctcaaggagc tacattctct ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg
    10981 gatcaagtgc gatgggcgct tcgatcttga gaaaaaaagt acaggaagca gacttgcctc
    11041 aaacggtgac caatcaagca atcagccaac tgacagatga ctcgaataca ttgatcgtta
    11101 ctcaggtcga actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag
    11161 tagaaaactt tttgaattca cctcgatatg atgaaatcat tcaagaatta agcagcaagc
    11221 agaaaacatc agtttctgaa attgagaatg agccagcaga agagtttctt tcagcaaaca
    11281 tcaaacaaat caaagagatt ctacttgtcc acgatgatcg ttccggatca gcaacaatgg
    11341 ggatgaccgt gctcaaagat atattagaca agaaccattt aactatcccg ttaagaaaag
    11401 tcaatatcca agatttaaaa gatgaacctg attcactgat cgtagccaaa gaaagtttag
    11461 cggaaaaagt acagatggct gcacctcaat ccattcattt atcagtcaac agcatggtca
    11521 atccgcaaaa atacgaatcg attgcacaat atttgaaaca gacagcttaa gggaggaaaa
    11581 cagcatgcaa agtttgccat tcgacatgat cgagctcaat cgatctttta aaaccaaaga
    11641 agaagcgatc cgctattgcg gtcagatgct tgtagaagca gggtgcgttg atgaggaata
    11701 tatcgatgcc atggtagata gagacaaaat gttatctgtt tatatgggga attttatcgc
    11761 gattcctcat ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt
    11821 tcaagtacca gatggtgtag attttggtac cgaagaagaa gaaaagattg cgactgtttt
    11881 gttcggtatt gcaggagtcg gagatgagca tttgcaactt gtacagcaaa tcgcacttta
    11941 ttgcagtgat atggacaatg ttgtccagct agcagacgcg ctcagcaaag aagaagtaac
    12001 ggaaaatcta gcaattgctt aaggagagag aaaaatgaga gcagtacatt ttggtgcagg
    12061 aaatatcggt cgaggattta ttggagaggt tttggcagct aatggatttg agattacttt
    12121 cgtagatgtg aatcacacga tcattgatgc tttaaatgaa agaaaagaat acgaaatcga
    12181 attagcagat gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca
    12241 aacaaatcca gaaaaagtaa tccaagaaat cgctgaagca gatttagtta ctaccgccat
    12301 tggcccgaat atcttacctt ttattgctga gttgattgca aaaggtatcc aagaaagaga
    12361 aaaggaagga agcacaacat ctttagatat cattgcctgt gaaaatatga ttggcggttc
    12421 agcatttcta gaaaaagaag tgtataaata tcttccggaa acatcattta ctgacaaata
    12481 tatcggcttt ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc atgaagatcc
    12541 tttattcgtc caagtggaac ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa
    12601 taaaaaaatc caattaaaag gggttttata tgtagatgat ctagaaccat atatcgaacg
    12661 caaactattt agtgtcaata ctggtcatgc gactgtcgct tatacaggtg ccttgttagg
    12721 ttaccagacc attgatgaag caatgcaaga tgcgttagtt gtggcacaat taaaatcagt
    12781 tttgcaggaa acaggaagtt tgctgattgc caaatggggg tttgatgcag agcagcatca
    12841 tgcgtatatt gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac
    12901 acgtgtcgct cggacaccat tacgtaagtt aggttatcaa gaaagattta cacgtccagt
    12961 gagagaacta caagaacata acttgacttg cccacatttg acggcaacaa tggggattat
    13021 ttttaattac tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca
    13081 tgaaaacttg gaacaattga ttcaagaagt cacaggaatc aatgatccga aaacaatcgg
    13141 gaatatcaag caaaacgtga accgttatgc caagcaagtc gcttaaaata ggatacgaaa
    13201 aaggggaatg tgacgaaatt tt
//
LOCUS       DAXLPI010000070        13096 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXLPI010000070 DAXLPI010000000
VERSION     DAXLPI010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.070"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(275..889)
                     /locus_tag="ACF3T5_001785"
     CDS             complement(275..889)
                     /locus_tag="ACF3T5_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFD3632386.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1495..1872)
                     /locus_tag="ACF3T5_001786"
     CDS             complement(1495..1872)
                     /locus_tag="ACF3T5_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632387.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2172..3053)
                     /gene="galU"
                     /locus_tag="ACF3T5_001787"
     CDS             complement(2172..3053)
                     /gene="galU"
                     /locus_tag="ACF3T5_001787"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFD3632388.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3096..4115)
                     /locus_tag="ACF3T5_001788"
     CDS             complement(3096..4115)
                     /locus_tag="ACF3T5_001788"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFD3632389.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4748..5752)
                     /locus_tag="ACF3T5_001789"
     CDS             complement(4748..5752)
                     /locus_tag="ACF3T5_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFD3632390.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5765..6814)
                     /locus_tag="ACF3T5_001790"
     CDS             complement(5765..6814)
                     /locus_tag="ACF3T5_001790"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFD3632391.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6831..7652)
                     /locus_tag="ACF3T5_001791"
     CDS             complement(6831..7652)
                     /locus_tag="ACF3T5_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFD3632392.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7639..8418)
                     /locus_tag="ACF3T5_001792"
     CDS             complement(7639..8418)
                     /locus_tag="ACF3T5_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFD3632393.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8435..8905)
                     /locus_tag="ACF3T5_001793"
     CDS             complement(8435..8905)
                     /locus_tag="ACF3T5_001793"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3632394.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(8917..9336)
                     /locus_tag="ACF3T5_001794"
     CDS             complement(8917..9336)
                     /locus_tag="ACF3T5_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632395.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9417..12194)
                     /locus_tag="ACF3T5_001795"
     CDS             complement(9417..12194)
                     /locus_tag="ACF3T5_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFD3632396.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12385..12609
                     /locus_tag="ACF3T5_001796"
     CDS             12385..12609
                     /locus_tag="ACF3T5_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632397.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3841 a         2740 c         2124 g         4391 t
ORIGIN      
        1 atatggattc tttcttgtct ctttttccac tactttttat ttttctgaca ctacatattc
       61 ttataactca tttctcataa ctgtgaaaca acttataaaa ataacaaact ttaaacaaaa
      121 caagcttaaa atcaaagatg atccatctta aaataactaa aacaaaaaaa gtagtcgttg
      181 ttaatcctca ttaataatcg ttttcttttt ttcaaacgct ccatttttat gtggaacatt
      241 atttttttaa ggaactttgt caacatgtgg ataactattt tccacttgtg tcctggacta
      301 gctgttttgc tgtaaaatac aaaacttgct gcagcaaaaa gatcgttgca agagaggctg
      361 ttccgcagat cgctggatct agatttggca aagtcaagac tgcatcatgt aaattggcgt
      421 ggttgtccaa aaccaaatca gcaaattcaa ataatcgcaa accactgcta tgttgcgatt
      481 tgatacttcg tgacaaatca aatccagtaa caatgattaa tggatgacca ttttctttga
      541 tccactgagc tagttcgatg attgccgggt ttcttccttc attcgaaaga agaagaaaaa
      601 tgtcttctga tttgacagaa agctgatcaa taattgcctg tcctgcacct tctagcgttt
      661 catagacacc tccggctgga ttttcaatca atatacttgg tacaagatcc ggggtttgat
      721 cccaaaattc aaatgctaca ccacttagtg tgcggctagc atatatctgc aatctaccac
      781 cttgattaat ttgtttcgcg atccaagtgc cagcttgaag catcagcggc tcttctatcc
      841 ttaatttctc ttccaattgg tgataatagt caaaataaca taaactcatt tatctctccc
      901 gcctagctat acttgacatg aatagaaaca gctgcctaat ctaaggaagt acataaaaaa
      961 acagtagtta gacagtaaca catgtggtct ttgctgtttc aaacacaaaa aaggcattga
     1021 acgaatacgc ccaatacctt gacatagcaa ataaaacaac gtgcagacca tggaaaatat
     1081 ctacataggg gtagaatttt caccctagat taggtacaat aaattaggtc gatgcactgc
     1141 attgactatg gcaaaatgat cggtcagggt gtatgtgtgt gactggtcat tccctgattc
     1201 ttagcgccaa ctaagggtca gggagccttt ttttattcag ctgtttctat ctgaatttat
     1261 tgtaagtaat ctcttatcca agtgcaagca agaaaataca aaaaaacatt ggtatgattt
     1321 atatatatat aaataacata aaaaaaggaa tggcttatcc cgatacaaca agcattcctt
     1381 taaaaaagat tcttttatat aagtgaaaaa agcttttttc caccaattaa ataaatctct
     1441 catctcaata gtttatccat tattttatct ttttttgcgt atacctacta aatattaccg
     1501 tcttcttctc gttcttctac tttgttcgag agaatctgta ctagaaaaag tagttgtatg
     1561 tctacctttt ctgcctatcc gagactttct tttcatatat gggatataaa tgattagata
     1621 cagtacagca aaaaataaag caaatacgca agcttcccaa ctacctaacg ccaattcaac
     1681 catcaaggta gcaaccccaa cggatgctgc ataccagata aaaatcagaa acgtttcttt
     1741 ttttctgacc atgcgaaaca tgataaatag actaataagc aatagtggca acacaaatat
     1801 ccaattgctt ccttccatca gccacttgag tatatcaagc agcgactgtg caacagcgac
     1861 taattttccc atagtgaaca cacacctttt tttataatta aaaaattcgc ctttttattc
     1921 tactatgaat cttctggctt ggctttcatt tctcttatgt aatcattttc ttttatacaa
     1981 cttaaaaaaa aattaaacca ttagcataat attttctgaa tctattcacg aaagtctttt
     2041 tctatctttc gttttccacg actcaactga tctagagtca aaaaactaaa acgacagttg
     2101 cgcaaatgac gcacaagctg ccgctttaat aacaaatagc ctttcattct cagcaggcca
     2161 cttatttcca gttaactttc tgttcttagc tttttactca actggataat atatgcactt
     2221 aatgaagtcc caatttccga atgttgaagt ccatattcaa tatttgtctt catatatcct
     2281 aacttatcgc ctacgtcgta tctttttcct ttaaactctc tcgcatagac cgcctctgtc
     2341 tggttaagag tctgaagtgc atccgttagc tgtatctcgt ttcctgctcc tggttcttga
     2401 gtttctaata tctcaaatat tttaggagtc agtagataac gtccgataat ggctaaatta
     2461 cttggtgctt ttcccatttc aggtttttct acaaaccctt ttactctgca acgacccttt
     2521 tcgctatctg ttttaggatc aataattccg tatttagatg tttcattttc tggtacacgc
     2581 atgacggcaa cagcggaagc ttgtgtttct tcataatcca tcatcaattg ttgagtcaat
     2641 gggatatcat ctttcatcaa atcatccccc agcatgacga caaacggttc atttcctata
     2701 aatgcttttg cttgaagcac ggcatccccc agtcctcttg gatgagattg acggatgaag
     2761 tgaagattga cttgagtcgt ttcttcaact aaagcgagta attctgtttt ttccttttcc
     2821 cgcaaattgt tttccaattc gatatttgca tcaaagtgat cttcaatggg tcttttttct
     2881 tttcccgtta cgatcaagat gtcttcgatt cccgaatcca atgcttcttc cacaataaac
     2941 tggatcgttg gtttatccac gattggaagc atttcttttg ccatggcttt tgttgccggc
     3001 aaaaaacgtg ttcctagccc agcggcagga atgactgctt ttttgacctt cacttgctgt
     3061 tcctcctaaa cgacgattaa ctattgatca tctatttaac gaagagaaaa ttcattctcc
     3121 atttttccgt cacgtaacat gatttctttt gctgcttttt tgatatcttc tccattatac
     3181 aaaacattat agattgtttg agtaattggc atctcaacac ctagctgttg agctagctct
     3241 actgctgctt ttgtcgttgc cacaccctca acgaccattc ccatgttttc tagaacttct
     3301 tccagcttat gtccctgtcc taacaagtta ccggcacgcc aattgcgaga atgaacactt
     3361 gtacaagtaa cgatcaagtc ccccacacca ctcaaaccga taaatgtcag aggattggca
     3421 cccatcgcga caccaagacg gctgatttct gccaatcctc gagtcatgat tgctgctttt
     3481 gcatcatctc cgaatcctaa tccatggata gcgccggcac caattgcaat gatatttttc
     3541 agtgctgcac cagtttctac gccaatcaca tcattatttg tataaatcct gaaataatca
     3601 ttcatgaaca gtttttgtac ataggctgct gctttttgat caatacttgc agctgtaatt
     3661 gttgtgatat catgtactgc cacttcctca gcatggctag gaccagataa tacaacaatt
     3721 gcctgtcttt tttcttctgg aatttcttca gccaaaactt ctgagattcg tttatgcgta
     3781 tcttgttcta gtcctttact tgcatgtata atgattggct tagtattcat tttaccaata
     3841 agttcctgtg cgactgaacg aatcgctttt gttggaacaa caaataaaat agcatctgcg
     3901 ttatccactg cttctgccaa ttctttatat ccgataatcg attctgggat cgtcaaatca
     3961 ggtaaaaaat gctgatttgt atgatactta ttgatttcat caatctgttc aggtacattt
     4021 ccccaaatac ggacatcatg cccgttttct gccaatgttt gtgctaaagc agtgccccaa
     4081 gatccaggac caagaactgc gattttttgt ttcatggata ttcctccaaa taaataattg
     4141 ataatttcca ttatagtata catttcttaa agttgggact aatttccatt gtttttaagt
     4201 gtttgtttac ttcttttatc gtttttactt gaaataatca atgatatgtt acaatagtct
     4261 acattttatt tttacactcg ttatgctttg cttgttccta agaggaagca gttttgacaa
     4321 tgttactacc agcattacta ttaggaggtt aggaaacttc tcctagtgag tattgattta
     4381 aaatacataa gagacagatt gaacatttct aaatctgtca ggtcctgcaa cggacagatt
     4441 gtctctgttg acggttgaca taggaacttt tgttttagtc aacattctgg atacaaaacg
     4501 ctatgtaaac tggcgcaact cgttgtactt ggacttttac tacggatcca tgcaacaact
     4561 aaatagtact attgcataac aaaaaatggt tggcacccac atgtcccatt aaacagatta
     4621 taagttgaac tgtagataat gcaaaaaatg tgttttataa cgtaattgat gccaatattt
     4681 taaaaaaata aagggagcag ttatcgctgc tctctttatt ttttatattc tttttatcca
     4741 gtctatccta ataatcaaac tgacgatagt agcggcgtat ctctaatgga tggcagttca
     4801 actgctccac atgagcatcg atgcgttgtg tcactacatg catcaataag aaagacaaaa
     4861 cacgtccgcg atatttttca ctgatacctt ctactggata gtcttttgtg tctatcaatg
     4921 tataattgct gcaaattttt ggcaaaagtt tagccacacg ttcagataac actctctgtt
     4981 catcttcgcc taaaaataat gtgactgggg ttgtttcttc tacgatctct aacgtcccgt
     5041 gaagaaactc ggcagcatgg atagatttcg atggaagcca gctttgttct tcccagtaac
     5101 acatggcata tgaataaaca gctccccatt gattccctgc tccaataaaa taatgcatac
     5161 tatcacgtcg atgtttttct gcaaatgata agccaaatgc atctgctttt ttctctgctt
     5221 ccactaaccc tgttggtaaa taacgttcga gctgttcata gtattctgaa taagcttcga
     5281 attctccatt caaatacatc aatcgatctg ctgccataaa aaatttgatt tgttctgttc
     5341 caggagctgg ataagtgacc acaaaatcgc atagctgaga caacttactg ttcgctttat
     5401 cgataaatcc aatcagtgtc gctcctactt tcttgatttg ttctactgct ttcactactt
     5461 cttgagttgt accggtaaca gaggacaaaa taacgataga atcttttgtt aatcttttgt
     5521 ttccagtagt gtaatattct gcagcatgct gtacaaagac aggcaagttg cttttcccat
     5581 tcatataagt gacagcttgc atcgaggaag catacgttcc accgattcct agataataga
     5641 cagcgtcaaa tttttccgca taaagtttat cgatgatttc ttctacttgc ggacgtaaag
     5701 ctagtgcgcc ttttattgct ttgatttgtt cttcttcatt gaatttcagc atgatttcca
     5761 cctcttataa tttacttttc aagactgtat caaagtcttc aaaaatcttt ttgttcaagt
     5821 caataccttt agctactgcg gttttataag cgaatagttg aaacggaacg acaagcgcta
     5881 atacagacag atattcatca cattctattg ctaatccaag tgttttctcg tttcttgctt
     5941 tttttgtggt aatggtgaga gtctctccaa cgtaccgact catatatctt tgcaactttt
     6001 gcgaacgttc cttatttaca ctttcccctt caataaagaa tagcaaatgc gaagcatctg
     6061 cctccaggta tgggccgtgc atataagctt ctaactcaaa tccttgtgac ggaacgcgga
     6121 ttgtttcagt caatttcgtc tctgcttctt tagctgttcc ccagtttgga ccatatccaa
     6181 tggtgacgaa acgctgtgct aaacgaaata aactttgata tccatcaaaa aattcttccg
     6241 ttttttgaat aatcgctggt aaatggttga ttattttgcg caactgttgc atgtagtctt
     6301 gttctatttt tttgctgatt ttatttttac ttattccaat tcctatccct aagagtagta
     6361 actgtaaaac agtcgcagaa tagccttttg taacgaagcc tactgattct accccggcac
     6421 ctaaatcaat aatttgtgca gcttctaaag caagtggact ttgcacgtca tttgtgatag
     6481 caacaacagg taacccttgt ttttgtatca tttttactgc ttcaacagta gaagcacttt
     6541 tcccgctttg tgacacagca atcacagtat cgattgaagg agaaagtttt ccataattga
     6601 aaaaataaaa aggttcttga atatcgacag taagatcacc ataagattcc aatgctggct
     6661 tcgctgctaa acaagcgtta taagatgaac ccgttgctaa gatcagtaaa ttcactgttt
     6721 ttttcatatg ttctcttgtc gaaaaatcat tctttttcaa gatcgtactt agtacttcct
     6781 tttcttcata gatataatct tgcatcgtaa acattttctc tgctcctttt ttaaaaaatc
     6841 cctatccacg aaccaaaaat tccaaagaaa gctagtccga tcaagatcgt taatggtttc
     6901 acttctttct tcaatagata aaacactatg ccaaatgctc caagacataa gatatttgga
     6961 acgatatcat caaaaatact ttgaacagtc acagcatttt ctcctgaacc gatttttaaa
     7021 ggaatattga tggtgatcat cgtggcagtc attgctccaa cgaccatcag tccaataata
     7081 gacgcgccta atgtcaaact ttccatcata ccgtttgcct ggatcttttt caaaaaaccc
     7141 gttcctaagc gatatcccca acctgttgct agatagcgaa acagaatgtg cggaatattg
     7201 aaaatcaaaa tgaacaagat agggcctaaa atatttcctt gcatggctaa agaggtaccc
     7261 acacctgtgg caatcaatcg caaagttccc caaaagaaag aatctcctaa ccctgcaaga
     7321 ggtcccatca aagatgtttt gatactatcg atcgcagtca catcaaattc aggatcatcc
     7381 ttattttctt cctccattgc agccgtgatt cctaaaatca gtgtaacgat atgtggggtc
     7441 gtgttaaaaa actctagatg ccgtttcaaa gctgctgcca tttcttcttt cgactgatat
     7501 aactttttca aaatcggaat cattgcataa acataagcca tattcatctg acgttcataa
     7561 ttccaagaaa actccatttg aaaagaacgc caaaatacgt gatttagctc ttttttcgtt
     7621 attttgctgt ttttgatctc agaattcttc atcttcttct acctcccctg cagttgtttg
     7681 aagtggtagt tgacggacat tcataatatt gactaccaca actgctaaaa tcgcaccaaa
     7741 aatagctact ccggtcacag gaatctctaa gtaagaagct atagcaaaac ctaagaaaaa
     7801 atatggtgca acttgtttgt tgatcagcaa acgagcaagc atcgcaaaac caagagctgg
     7861 aatcaaccct gtagctactg acaacccctt ttgaacgaag tcaggaatca tatttaatag
     7921 atttccgatc gtattgcttc ccaccatata agaaaccatt accacaacac caagcatcaa
     7981 ggaaagaccg ataccggcaa gcaagtgcat atgctcgact cctttataat ttccttcttc
     8041 tgcataattg tctgctttat ggttcataat tggaatcaaa agccctaagt aaatattttt
     8101 caatatcaat gtcaacgtag caatcggcaa acctagtaaa agcgcggttt ctgtaccggc
     8161 tccagctgta atagcaaaag ccgtccctaa aatcccaccg gtcacaacat caggtggaat
     8221 cgaagcacct actgagaacg atccgataaa tgctaactct aaagttgcgc ccataatgat
     8281 cccagttttt acatcaccta acacgattcc tgtaaacagc cccgtcacaa tcggacgaga
     8341 aagcaacgat gtccctagtg catattctga ttgtgcgaca aatgcaacaa ttcctaataa
     8401 gactgcttgt acaatcattg aggttcttcc tttcttacaa aacttctctt acatccactt
     8461 ttttatctgc tggtacttga cggatttcta cttctatttg tttgtcaatt aatcgtttca
     8521 agcgttcctc ctcttctggt aaaatattta ccgcttttcc gatgctgcga gtaccaggtt
     8581 ttgccttgat ccctcctaag ttgatggttt tgatttctgg atacgcttct gccagcttat
     8641 atgcatcctc gatcgattcc actacgatga ataacttata tttatctgtc accccgcttt
     8701 tcaacgcggc aatcgagtca tcaattgatt tgatcactag tttgactcct tgtggcttgg
     8761 ctaatttgat cgttgtcttt cgtatctcat tcgtaggtac atcatcatta gcaatcaaaa
     8821 tacagtctgc gcctattgtt tgtgtccatg aaaaagctac ttgtccgtgt aataaccgat
     8881 ggtcgactct tgtcaataca atcatttttc ttcctcctaa aattcgtctt cttctatttt
     8941 ctgattgagc cgctcgttac aaaactggat catttttttt gaatcagtta gcacctgctg
     9001 gatcaaaaca gttgtctcta cttcagcgtc caaccgagat actagctcaa tcaatagtgg
     9061 caaattcaat ccggcaacga gatagacatg atcccgattg atatattgta agaattcgtt
     9121 gttgacactt ccgccaaaaa gatccgtcag aacaatgatt tctttatctt tgtacctcat
     9181 aaaaagctgc cttactgttt ccgctaaatc aaaattctca tcagtatagc aatcgaatac
     9241 agtaatagaa tgatttttcc catagatcag ctctaatgaa tctaatatcc ctgaagcaaa
     9301 ttttccatgg gaagctaaga taatttctct atccactttt tcatctcctc ttttcactta
     9361 cacatactaa aaaagcaagt tgcatgccaa aattagtggc aacaacttgc ttatgttcaa
     9421 aattcttctt ctatcgagag aaactctgta tcttcactca aaatatcata gatataggca
     9481 atctcagaat tggggatcat gacactataa tgctcttcaa tgacactaaa cgcagattta
     9541 atagcagata acccttcttt ttgacactgc ttccatttct ctaatccttg ataggtttcg
     9601 attggcattt gtcggatcaa tcgttcaatg agacagctca catggacata caacgctaat
     9661 tttttgggat tgctgatgct tcgattcaac tgcacctcta attctctcat aaaatgatcg
     9721 atttctccca tcaccttctc tgtatctaga atagtcacgg aatccagtac tttttccaat
     9781 gaaaaattac gaatgatgcg ctgattgaat atttccttct ctgaagcatt catagtagtg
     9841 tccaaccatt cagctagtac agacatcttt tcccccgaga tgaggtcttc taatgaaaga
     9901 tagtcgatat tggtgatatc aggatctgtg gtgccaatca ttcctacgac ttcgtatatg
     9961 gaaaataatg ggtcactgtt tttcttatct tttaattgct gatattcata tgggatgatt
    10021 ttcaactgac aagccgtagg taagcttttt tctagcagtt cacttaagtg gaccgccgta
    10081 ccgattccag tgaagcaagt agtaatgagt gctttgggtt tgttttcctc tggatagagc
    10141 agtcgccatt ctggctggat ctgtcgaaca gtctcttctg ctatctcttc taatgataca
    10201 tttttttgca ggcattcccc tactgtgatt gctaaaggtg tcgtcacatt attcaaaatc
    10261 aaaagcggta cggataactg ttttttaaaa aactgatgga tttctttcag agatcccatg
    10321 tctactaata tgatcaatcc atttgaaaca tcgctgtttt cgcaataatc aaggatttct
    10381 gaagcaatct gctgtggtgt gacatctagt ggcatatcaa atgattcaaa tagatcctct
    10441 tgtaataaac gatttactac atttgccata ctgctagcag ttgcataacc gtgagcagca
    10501 atcatcgctt ttggcagtcg tttttctttt gaccatttcg ctttttttag atagatcgtc
    10561 agtaaaatcc gatccatact tgtgtcttca atatctaatt tgatcttact taattccaga
    10621 attcgttgga catagtgata actagatgga taagactgag aaacttgttg ttctaattct
    10681 ttgattagtt ggataatatc atgttcttcc ggtaaccatt tgatagtgct tcgctgatag
    10741 agatagtaac tcaaggcata gacactgttt ccatcgaatt tgatttgata ggcagtttcc
    10801 atttgtcgaa aggtctctcg gatagattgt gtccaataaa ccagcagctc gtgtttttct
    10861 tgtctactgg tctcaaacag caaaaaatca aataattgaa tcacttcttg ctttaatttt
    10921 ccttcgcaag aatatagcag acagttattt ttcttgaaat caattaggat ttgctcaaaa
    10981 cttttaacga ttcgctgctg ttcgggctgt tgcttctcca ttaattgctc taaggtctgg
    11041 tcttgcgtaa tatagattcc ttgctgtgct gtatttttag taataggctg cgcaagtagt
    11101 tctttttgca aatgatggat cgtgacatgg atttcaggct gttcacgctg ttcagcatat
    11161 gcttttgctg ctgaaacttt gacgatattt ttcagctctc ctatgtttcc ggataatttc
    11221 tgacttgtta aaagtgacaa agctggtcca ctgactgaca aagggctccc catttttctt
    11281 tgttcttcga gaaaaaaaga gtaaatcagt tctagccgtt cattacgact acgctgatta
    11341 agtgcaggta gttcgattcg gataggtatt cttcgaatga aagttgttaa gaaatgacta
    11401 gtaatctctt cagtcgtagc aaaaaagaga cgtgttttga tccggcgtga caaattaggt
    11461 tctcccatac gataaatcac accttgatca agataggtga acagtttttc ctgaccttct
    11521 ggtgttaagc gatgtacctc gtctaaaaac aagatcccac catctgctgc ttcaaaagcc
    11581 ccttttttat tttcttctgc accagtgtac gctcccttaa catgtccaaa caaattgcta
    11641 gtcaaaagtt caggattatc tgcatattgt gcacaattaa aagtgatgaa tggtgcagaa
    11701 tcttctagca gatcatgaag cagacaatac tgatagacca aattaactaa ataactttta
    11761 cctgtgccac tttcgcctgt aatcaaaagt ggtaatccgc catctggata gtaaagagct
    11821 gttttgattt gttcgatact ttctttcaaa ctggcgtcgt atccgatcat aaaagagaat
    11881 aagtcttttg gttgcgcgaa aaaaggctgt tcctctttga gatcatcgat agaaaaataa
    11941 aaattcgtgc gtaacttaaa aaattgtttt tcaaaagctt ctttgtggaa ataaatcact
    12001 ggtcgagaat tgattttgac tagtagttgc tcatcattta actggttcaa ataatgactg
    12061 actgtgtttc tttttacccc aaaatgatca gctaaccttt gtgctgtaaa gacctcgttc
    12121 atatgttcta aatcgaaaaa ggctgtctgg ttctctaagt aacataaaat ttccttcttc
    12181 aacaacttat tcacccctcc tccatcatag aaaacggatt cattttgtca ataaaatctt
    12241 taataaagaa ttgaaaaata tatcttatta tagacagcag cctacttatc ccttataatt
    12301 tttattctaa aaaacgtaca gttccttcca gtttttatac tggaatttca acatatgaaa
    12361 gaataaggag gctatgacta ttcaatgaaa tcggataaat ataaaaacag gaaacatact
    12421 tcctggaaaa cttttcgaga aatcatcgga tcttatctcg ctgtagttgt atttttgcct
    12481 ttgctttata tggctatgac agaacaaact cgttttattg gcgtagatac gcaatatata
    12541 gccgaaacag gatttttatt catctttatc ttgatttttg cgttctataa atccttccat
    12601 acaaaataag attcacagat ttcaactttc ctgtctgttt ttagctatta caaatcctta
    12661 tatgtggtat aacaacaatt tattggcata atgaggaaaa acgagttatg atgctttcag
    12721 agagtaaaga ggtactgttg tcaccatcct ttttattgat tacgtacacc actcttagtt
    12781 ttttcccatt gattacgaaa acaaggtaga aacattgatg ttcctacctt gttttctttt
    12841 tttattttca gatcaaaatc tcaagtagct tttgacttga tgtcgggagg ttgtcttcgt
    12901 tccatcaaac ctgatagaat ctgcgccaaa ccatagtttt ctccactatt atttattcct
    12961 ctaagatagt acccacaata aacgaataaa tatttttctc attttcatac tccttctttg
    13021 aaaatcaatt agatttctct caacccactt ttttacaaaa attacgtagc aaaaaagatt
    13081 gcaagaacaa attaaa
//
LOCUS       DAXLPI010000071        12621 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXLPI010000071 DAXLPI010000000
VERSION     DAXLPI010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.071"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            2..2374
                     /locus_tag="ACF3T5_001797"
     CDS             2..2374
                     /locus_tag="ACF3T5_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFD3632398.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTLTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2435..3895
                     /locus_tag="ACF3T5_001798"
     CDS             2435..3895
                     /locus_tag="ACF3T5_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFD3632399.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            3906..6110
                     /locus_tag="ACF3T5_001799"
     CDS             3906..6110
                     /locus_tag="ACF3T5_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFD3632400.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            6126..6275
                     /locus_tag="ACF3T5_001800"
     CDS             6126..6275
                     /locus_tag="ACF3T5_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632401.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6351..6944
                     /locus_tag="ACF3T5_001801"
     CDS             6351..6944
                     /locus_tag="ACF3T5_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFD3632402.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6957..7856
                     /locus_tag="ACF3T5_001802"
     CDS             6957..7856
                     /locus_tag="ACF3T5_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFD3632403.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7859..8344
                     /gene="ssb"
                     /locus_tag="ACF3T5_001803"
     CDS             7859..8344
                     /gene="ssb"
                     /locus_tag="ACF3T5_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFD3632404.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(8485..8589)
                     /locus_tag="ACF3T5_001804"
     CDS             complement(8485..8589)
                     /locus_tag="ACF3T5_001804"
                     /inference="COORDINATES: protein motif:HMM:NF028245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFD3632405.1"
                     /translation="MPVETALGLMISFGSLIATIIFGILAVVNNNQKK"
     gene            8763..9023
                     /locus_tag="ACF3T5_001805"
     CDS             8763..9023
                     /locus_tag="ACF3T5_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632406.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            9460..9666
                     /locus_tag="ACF3T5_001806"
     CDS             9460..9666
                     /locus_tag="ACF3T5_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFD3632407.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            9666..9917
                     /locus_tag="ACF3T5_001807"
     CDS             9666..9917
                     /locus_tag="ACF3T5_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632408.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            9932..10369
                     /locus_tag="ACF3T5_001808"
     CDS             9932..10369
                     /locus_tag="ACF3T5_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632409.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            10362..11069
                     /locus_tag="ACF3T5_001809"
     CDS             10362..11069
                     /locus_tag="ACF3T5_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632410.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            11234..11434
                     /locus_tag="ACF3T5_001810"
     CDS             11234..11434
                     /locus_tag="ACF3T5_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632411.1"
                     /translation="MNKIIPLLFVLFVGEILMRHGAALHLNFVVFLGILVFCLIFWRF
                     VLTLVSLIFKLIAAIFLIGLFR"
     gene            11450..11629
                     /locus_tag="ACF3T5_001811"
     CDS             11450..11629
                     /locus_tag="ACF3T5_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632412.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11760..12029
                     /locus_tag="ACF3T5_001812"
     CDS             11760..12029
                     /locus_tag="ACF3T5_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFD3632413.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            12022..12279
                     /locus_tag="ACF3T5_001813"
     CDS             12022..12279
                     /locus_tag="ACF3T5_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFD3632414.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT         4745 a         2059 c         2612 g         3205 t
ORIGIN      
        1 catggcagaa gaaaaagaac cactagacaa tagtcgcttg ggaaaatcca aaagaaaatt
       61 agtacgattg caaaatgaat taaacgaaca aattgagaag atgtttgaac accagagaaa
      121 gaccaacggt cagccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      181 acgtttagaa aacaaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaggt
      241 agaaaaactt gaaagacaag aagagctaaa ggaaatgggc tataacaagt acggcggcct
      301 ggatatgaca atcgaaaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      361 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      421 acagctgaaa gaaagatcag aaaaaggaaa agaaaatctt cttcctgaag tacaagcaat
      481 tattgacagc ggacgagtga cgcaatggaa aaaaaatcca accatttatt ttttgaaaga
      541 ctatcgaaaa gttgctttag aactgaatga aaacggggga tttgaggaat caaaaaaata
      601 ccaagccacg tcggaagagg aaaaagcagc tgttcgtgaa ttacttgaag aggctgcgcc
      661 aactgattct ttgaaggaga aagaaaacga tcatttctat cgagtagaat tcaacgaaaa
      721 tgatccagaa aaaggattga aaagttatgg tggggagatc gtaacattag aattgatcga
      781 tcaactcaaa acgttggata accaatacgc cgcagaagca ggttattata aattctattt
      841 tgaagaagtg aagaacggag aagtagtcga tcagatccga atggatctag gggatggcgt
      901 caacagtaat caacccatct atttagagtt agcaaaattg tgcgaacaag tacctgaaaa
      961 ggaacaaaaa caaacggtca aaaatgaacc tgtttcgaaa gaacaaatgc agtcgaaaga
     1021 aacaaaaaca aaacaaaagg gctatcaaaa acgaacggca gaagagatta agcaagaagt
     1081 caaatccctt tcgcaacagg cactggatgc ggtgaaaaag catacccatt ctcctgaaga
     1141 cgtgaaagaa cttttgaatt ttatgtctcg ttttccagaa cgatccttta gaaatcaatt
     1201 attgattgag gaacaatttc caggtgcaac ggcttgttta ggtcgtgctg cgttgaaaaa
     1261 agaaggaatc tatatcaaaa aaggggaaca agggaacaag atctttgtca gaaaaacggt
     1321 caaaggattt tatgagaaag gacgtggctt tgttcgagaa acagaagcta caccagaaga
     1381 taaaaaacga atcgaaagtg gtcaaatcga agtgatcaaa aaaccttact acacgattga
     1441 aaaagtgttt gatatcacac aaacacagtt aaaacctgaa gactatccga aaatctttcc
     1501 gaaccgtgtc tttgattttc aattggataa aaaaggacag tcagaacttc aagctgggat
     1561 tcaagcggta gccaacaata ttggaataca aattcgagat atgacagaaa gtgaagtcta
     1621 tcaaagagaa ttaggacaag cacggggggc gtatgttcgt aacgaattta ctggcgaaga
     1681 agaaatcgtc atgaatactc gaaatactct tacgcagcat ttagctacaa gtattcatga
     1741 attagcccat gcaagaatgc acaaattttc agaattagat accgcaacta aagaattgca
     1801 ggcagaaatg acttcttata ttgtgtgcaa acattttgga atggacactt cggagaaagc
     1861 tattccttat attgcagcat ggacgaagaa tggtcagacg ttggatgata aggaagcggc
     1921 ggaacgtggg aaaatcatga atgatgtcag ccgtgtcgca aatgaattca ttcaaaccat
     1981 ttctaatgaa atcaatcaat acagagaaag agaaccagag atccaaccag cagaagtgaa
     2041 gcaggagcat aaaaatactg tagaaacggc agagaataag aatattgtgc ccgttggttc
     2101 actttggatc gacaagaaag acgggaaagt aatggaagaa gatacgggtc gaacgttaca
     2161 tttgaaggat gcggcgttta ttgaagatcc gcagcataat caagtgattt taaaaggaaa
     2221 aagttatgga aaagagattc aaacggcgtt accttcagaa gaatttgcgc taggaaatcc
     2281 attccgattt tcacttgaaa cagtgtcaga agtggccaaa cgaaaaaatg aaatctatta
     2341 tgaacaacaa ttgtcacaaa aagaacgcgt ataaaacaag taaaaaagac tatctgaaaa
     2401 acggatagtc ttttttcttt tgggaggttt gaaaatgacc gaaaaaaaaa tgagtcttat
     2461 tgatcgatgt aaacagatcg atatagtgga ttttgctcga aacaatggaa tggctgtagt
     2521 caataaagga cgagactatc gcttagaaga ccatgattca tttgtttttg atcgaagaaa
     2581 acaacgtttt tattggaaca gtcaaaatat tagtggcgac attattgaat tagctaaact
     2641 ttttttcata gacaaagaga tccaagactc taagcaacaa tttaaagcag ccacagattt
     2701 tattctaaag aatgaagaca aaacagaacg agtagaaaat cttcattttg agacagaaaa
     2761 gtacaaagat caccccgttg attatcaacc actcacagaa aaaggaagga attatctgaa
     2821 ggaagaacga aaactaccag agtggctgat tgattatgca gaaaaagaag ggttgattgc
     2881 cgaactaaag ccaaaacatg aacggcaaaa tttcttagtt cgagacgatc gtttagatca
     2941 tgcggtcgcc tttttatgga aagatccaca gacaaatgag acagtagggg cgagttatca
     3001 aggaacgttc attgattatg aacgttttgg tgagagaggc acatataagc atatagataa
     3061 aaattcgaca gcgaatcatg gctttaacct aaagatcggt gaccctaagc agttaaaatt
     3121 ttttgagagt agtattgatt tacttagcta tgctgcacta aatcgtgatc agttaaacga
     3181 tacgtggtta gtttcgatgg aagggttaaa acaccatgtg atcagtcatt attttggtga
     3241 ggcagtatct gaattgagaa agaaacaagc ctttcctcaa tccatcgaga tttgtgtgga
     3301 taatgaccgt gcaggacata ttttctacga aaaagaacaa ttaatgggcg cagttgatcc
     3361 atttacgaat cagaaagtac gttgtgaacg aggaatcgct aatgattggc aagtgccaaa
     3421 agaatacaaa gtcatttatg aagaagtcgc aaaagaagaa aagatcacac cagaagctat
     3481 tatggccata cacaagacag aaaacaatct gcaattaacg aaccaattgg tttccgctca
     3541 caaagtaaat gcgtcttttg gtcagcagct ttctgttaac gattcaatag aagctattaa
     3601 tttgaaagat atttgtagag aagtcgcaaa agaattaaaa gcttgtgaac gcgtggatgg
     3661 tacgtacgat tttgatcgtt tttatcaaga aaaaggggat attaatgctc agattctttt
     3721 ctcttataaa gcagaacaat actacaaagg ctataaaaat catgaacatg aatttgtacc
     3781 tgaagtaaaa aaggattgga atgatcaatt gaagcatgaa atctaccaac aagagatcag
     3841 aaagcaaaaa cgtgccatgt tgtttcaaca aggtagacag caagaaaggg agtgaaatcg
     3901 tcatgatgaa aaccgttatt ttagcagaaa aaccgtcaca agcgaaggct tatgccgata
     3961 gcttttctaa agctacccga aaagatgggt attttgagat ccaagatcga ttgttttcag
     4021 gagaaactgt gatcacgtat ggttttggtc atttagtcga gttagattcg cccgatatgt
     4081 acgatgaaaa ttggaaacaa tggtcattag agcatttgcc tattttccct acacattatc
     4141 actatcatgt tcctaaagat aagaaaaagc aattcaaggt ggtgaaacag caacttcaat
     4201 cggccgacac cattattatt gcgacagata gcgatcgtga aggggaactg atagcttgga
     4261 cgatcattca acaagcaggc gccgatcatg gaaagatatt taaaagactt tggatcaact
     4321 ccttagaaaa agaagccatt tatcagggat tccagcagtt acgagatgca gaagagacct
     4381 acccaaaatt tgaagaagca caagcacgcc agatcgccga ttggttgatc ggcatgaacg
     4441 gtagcccgct gtatagttta ctcttgcagc aaaaaggcat accaggaagt ttttccttag
     4501 gcagagtcca aaccccaacg ctttacatga tttatcaatt acaggaaaag attagaaact
     4561 tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat ggcgcatttg
     4621 atgcgaagct tgatccgaat gaaacccaag cgacgcaaga agcatttgaa gattatttaa
     4681 aagaaaaggg ggttcaattg ggaaaacagc caggtacgat ccaccaagtt gaaaccgaga
     4741 aaaaaagtgc ggcaagtcca cgtctttttt ctctatccag ccttcaatct aaaatgaatc
     4801 agctcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg tatgaaggga
     4861 aatatctaag ctacccacga acagataccc cttatattac agaaggggag tatgcgtatc
     4921 tgttggatca cttagacgaa tacaaacact ttttaaaggc cgaagccatt cctactccta
     4981 ttcatacacc gaatagtcga tatgtgaata ataagaaagt ccaggaacat tacgcgatca
     5041 ttcctacaaa gacggtcatg acggcagcgg cttttgaaca attaagtcca ctgcagcagg
     5101 ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag tatacctatg
     5161 aagaaacaac gatcttgacg caagttcagc agcttcagct aaagacagtc ggaaaagtac
     5221 cagtagatct aggttggaaa aagctgtttg gcaaagagag cgacagaaaa gaaaaggaag
     5281 aagaaccgct acttcctaaa gtgacaaaag gagaagcggt gacggtagat ctacaagtcc
     5341 ttgaaaaaga aacgaagcca ccacaacctt ataccgaagg tacgttgatc acagcgatga
     5401 aaacagctgg aaaaaccgtg gatagcgaag aagcacaatc catattgaaa gaagtagaag
     5461 gaatcggtac agaagcaaca cgagcaaata ttattgaaac tttgaaacaa aaggaatata
     5521 tcaaggtaga aaaaaataag cttgtggtga caaataaagg gattttactt tgccaagccg
     5581 tggaaaaaga gccgctgcta acaagtgccg aaatgaccgc caaatgggaa agttacttat
     5641 taaagattgg tgagcagaaa ggaacacaag ctactttctt agctaatatc caaaaatttg
     5701 tctctcactt gttggaagtc gtaccgggac aaatccagtc aacagatttt ggatcaacgt
     5761 tacaagaggt gaaagcagcc agtgaaaaac aggaagcaac gcgtcattta ggtgtttgtc
     5821 caaaatgtcg ggaacaagaa gtcttgcttt atcaaaatgt ggcggcatgt acgtctgaag
     5881 cctgtgattt taaattatgg acaacgattg ccaaaaagaa actcacagcc acacaactga
     5941 aagagatcat tcaaaacggc cgaacaagcc aatcagtcag aggactaaaa ggtcagaaag
     6001 gttcgtttga agcaacgatc gttttaaaag aagactttac aacaggcttt gaattttctg
     6061 aaaagaaaaa aacgaactat aagaaaagaa cacgccgcac tacaaaataa tgaaggggtt
     6121 atcctatgcg aaatacaaca cacaaacaac aagaaatcga aaatcaaaaa gcaaaaattc
     6181 gtttagaaca agagaaatta cgtgcaaata aaagaagact gcaacaatta aagaaccagc
     6241 agcgcattca aaaagaaatc gaattagatc gatagacgtt aatcttgtta caaataaact
     6301 actatgatat atttgatgta gtgtatttgt aacgaaggga ggggaagtaa atgcaagata
     6361 aactgaaaga attgttggct attcgcgatg gaaatttatc tatgaaggaa gccaggaaat
     6421 ttggaatcgc agcaaccact gtacaaagat tggtgaatca aggaaaattg atcaaagttg
     6481 atcgtggtta ttatgtagag gatggtcatg gaatagatga tttattttgg cttcagcaac
     6541 gattttccag gggaatcttc tctcatgaaa cgactttaga tatttaccag ctttctacga
     6601 atatgccgaa attcattcac ttaacttttc cccatggcta taacgttgat cgttcaatta
     6661 caaaagagca gcagttacaa tttcatttcg tgaagaaaga agtttatgaa ctaggaaaag
     6721 tagagggaac ttcctttcaa ggaaatccta ttactatgta tgataaagag cgtacacttt
     6781 gtgatatttg gaatccgaga tatgaatttg aatatgaaat gaaattagaa gcattaaaag
     6841 agtacatgga agacccattg agagatccaa gtaaattaag agattacatg acaaaactac
     6901 atgtagaaaa agaaatgaaa taccacatgc aaagcttata ttgatggagg aaaaagatga
     6961 caccgacaca atttgcagca aaaacaagaa atttagcaaa gagtacagga ttaaatgcac
     7021 agttagtgca acggcatttt atgatggatc gtctaattga acaaatttct gaatcaaagt
     7081 acaaaaatga cttcattttg aaaggcggct ttttattagg atcaaaatat ggaatcgata
     7141 gaaggacaac aattgatatc gatacgacat ttagaaatag caaattaact gaagaaaaat
     7201 tgaagagcat ttttaatgat ttaactagaa aacccacaaa agaaggcatt gttttctcta
     7261 ttcaaggaat gaaggaaaca agagaagccg atttttatcc tggatttcaa attaaattgg
     7321 tggctaattt agagaaagta cgtgtaccgt ttaaaatgga cgtgacaaca ggagattcta
     7381 tttatcctga agtagataca cactatcacc aattaatgtt tgaagataaa aaagtggaga
     7441 ttccagctta tccaacagaa caaattattg cagaaaagct aagtgctacg ttcagctttg
     7501 gcacagataa ttcgagagca aaagattact acgacttatt taccattccg aaactagaaa
     7561 atatcgatac gaaagagtta tacaaaagtg ttcgaaatac atttacaaaa cgtggggata
     7621 caaaaccgtt gtccttttat tatgaaagag aaatggataa gatcagagga aatgactatt
     7681 tggaaaagca gtgggaaaaa tatcgctcag ttaacacgtt tgcgaattcc atttcattta
     7741 cagatacagt caacgaaatt gatcacctaa tgaaaagtgt gatcaaaatt gaaaatcaag
     7801 aaagaaaacg gcagtttaaa agatcacaac aaatgaatca agaaatggag agataaagat
     7861 gatcaataat gtaactttag taggacgctt aaccaaagat ccagatttac gttataccgc
     7921 gagtggtact gcagtagcga cttttaccct agctgtcaac cgaaatttca cgaatcaaaa
     7981 cggcaatcga gaagctgatt ttattaactg tgtgatttgg cgcaaaccag cagaaacgat
     8041 ggcgacatta gctaaaaagg gcagtttgat cggcgttgtt ggacggattc aaacccgaac
     8101 ctacgataac caacaaggac aacgtgtgta tgtgacagaa gttgtggccg ataatttcca
     8161 actattagaa agtaaagcag cgacagaaag ccgtgcgcac gctgatcaat cgtccagcag
     8221 tccaagccct accacctttg aacaacggga tgtagctacg cctaacaaca atggattgaa
     8281 cgcttcacaa aatccatttg gcggacaatc aatcgatatt agtgatgatg atttaccatt
     8341 ctgattgtaa aagatcaata aagttgttat aatggatacg aaaagaggag ttgcgctaac
     8401 aactccccta acagcaccgt ttaagacggt gaccagctta atattatttt tgaaaaataa
     8461 tcgtcgaccg gctaaagctg acggttattt tttttgattg ttgtttacaa ctgctaagat
     8521 tccaaaaatg attgttgcga tcaaagaacc gaaactaatc attaatccta gcgccgtttc
     8581 aacaggcaaa aggcttctcc tttctgtaag attttggaag tacgcacata cgcaccacct
     8641 cactttcaat cggatagcca ccgtcacaaa ctttactgtc ttgatcatta tagcatatct
     8701 aaaatcgaat aaaaattctc aattttagct gtttttgcta taattttagc gaggtgatcg
     8761 tgatgaacaa atatgttggt ttgcttgata aaatccgagt aataaaaaca caaccattat
     8821 tggttcgctt tactttacaa acaattcatg aatcgattaa ttgtgtagtt gcagatattg
     8881 aaattatcga taaattgtta attatggatg atggaaaata caatattgcc gtcacaggtc
     8941 atttcaataa acggaatcaa ttagtcattg aatcgatgca aattagaaac cccgatcatt
     9001 ttacgcgatc gatgggcata taaaagaaga gctgcagtcc taaatgagga ttgcagctct
     9061 ttttgtttta catagaaaca ggaaaagacc tttttaggaa gacacaagtg ccttctcaat
     9121 tgaaaaacct ttctctcact ccaaccgcca ccgccgttcg agaagaaagg aagtgaacgt
     9181 tgcacgttca ctaagatatg gtgtcttgca aagcaagaca aaatagaaaa aacttcctct
     9241 caccaaaagg ttcgttcctg ttcgctatct ctttattgat ttgccagacc ggcaacaagt
     9301 tgccttttat caccaaaaag cgacaaggac aagggtaaat ggcacgctgc gcgcccttgt
     9361 ccttgtcgct tttcgttgat tcattctggc caacaaatca ataaagaaag aactaacgaa
     9421 aggaaataaa acctttccta agaggaggca gaatcaatca tgcgaaagaa atttgattat
     9481 tggggtgttc ccttcagcga attattccca aactatcatg caccacatac tgtcgaatgc
     9541 gattgtggag aaagagcgaa atgtatcaaa agctatcgtt tgtatcaatg tccaacatgc
     9601 gggaaaaaat ataccttgtc ttatggcgat tatgttttga ttgatgagaa agtaaagaaa
     9661 cggtgatgaa aacacaacac aaattattga ctgtaaaaga agtcgttcaa attttggtgg
     9721 acaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     9781 tagaaacccc tttagtagag gatgcaccga ctatgctatt tgaagaccta gtgaagattg
     9841 caggatatga actgcattat caagaaaaca tggaaactta tgaaatcgaa tattggttaa
     9901 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcaa aattactatg acgaatttgt
     9961 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
    10021 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
    10081 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacactgg aagaactgaa
    10141 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagtag gtgtcaaacc
    10201 aagcactatc aaaccttccg aatatcaagc cttagaactg acttatgcaa aattcgcaca
    10261 gattaagaaa gctaaagcaa tggctagcga ctggctagat acttttgaaa atatcgaaaa
    10321 aaatggtgca ttttatacga ttgaatcgga ggagaaagat aatgaataag gtggtttatc
    10381 agctaacaac agaagatgtt tcgcaatttt taacaaacaa agaacacttg aatgaagtaa
    10441 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
    10501 tggaagaaca gttgtatatg gaaagtgaaa cgagtcaaac gaaggttaca gtcagccata
    10561 atcgtttaga cgtgtataaa cgtcgtcctg acacattaaa aaaaggagcg gagcttctac
    10621 gactagaaac agaaaaatat atcatgaatg tttacctaga aaggagtgag ctggtcgaaa
    10681 actttgtcag ctacacgatt tgcaggaaac actattactc agaatcagaa tattattcaa
    10741 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaagatgcga
    10801 cggtagacga ttattacctt gtatttacta gatttttgac gtgtgggacg gaatctatcg
    10861 tgttaatggg aatggattat gaactcactg tacccgaagc atttttgagg gaatatgtag
    10921 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagacacct
    10981 acacttacga cgatacagaa gaaatccaaa atgaacttga ccgtaaacga atgccatatc
    11041 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag gatgtgtgaa
    11101 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgatccaa
    11161 ccgcccccgc cgcttaggtg gtgcgaaata agcgggcaaa gcccgataac ctaagagaaa
    11221 gaaggagatc catatgaata aaatcattcc tctattgttc gtactgtttg ttggcgagat
    11281 acttatgcgt catggtgcag ctctacattt aaacttcgta gtattcttag gaatactggt
    11341 attttgtttg atcttttggc gctttgtgtt aacccttgtg agtctaatat tcaagttgat
    11401 tgcagccata tttttaatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
    11461 aataggaaat aaacggttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagca
    11521 actaaagcag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
    11581 gattgcccaa gaaaaagcag gatttgaggt accagcactc aaaaagtgac gtcacttttt
    11641 atcctaaaaa ctaaaagtga tagcactttt aattataaga agttagaata ttaatcattt
    11701 gcttaattgt acaatataat gtacaattgt tttatagaaa taaataaggg gtgaaaggaa
    11761 tggaagcagt agcttattca aatttccgcc aaaatttacg tagttatatg aaacaagtta
    11821 atgaggatgc tgaaacactt attgtaacaa gtaaagatgt agaagataca gttgttgtat
    11881 tatcaaaaag agattatgat tctatgcaag aaacgttgag aacactttct aataattacg
    11941 tcatggaaaa aattcgtcga ggagatgaac aattctccaa aggtgcattt aaaacacatg
    12001 acttaatcga ggttgaatct gatgattaag gcttggtctg atgatgcttg ggatgattat
    12061 ctttattggc atgagcaagg aaacaaaagc aatataaaaa agattaacaa gttaataaaa
    12121 gatatcgatc gttccccctt tgctggatta ggaaaacctg agccattaaa gcatgattta
    12181 tctggaaaat ggtccagaag aattacagat gaacatagac tgatatatag agttgaaaat
    12241 gaaacgatat ttatttattc tgcaaaagat cactattaac caatcggaag taaggaaagg
    12301 gtcagaaact taaaagtttt tgatccttat tttatttacc ctagtcattt aaaaagctaa
    12361 tatagcttag tgttgattgt tattaatgaa tgtgtttgtt acgcgtatta cggatataag
    12421 gttagtaaaa tcatttctaa agttgaggaa aagtaaatat aaatggctta aatttcaaca
    12481 atttgaagtt gaatagatat gttataatac tattgtagtg tgggatgtta gttactaaag
    12541 gatgacgctt atatatatga ctgaatagaa taagcaatag gtttaataat ctattttaaa
    12601 ttttttgtac tagttttagt t
//
LOCUS       DAXLPI010000072        12344 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXLPI010000072 DAXLPI010000000
VERSION     DAXLPI010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.072"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(180..737)
                     /gene="frr"
                     /locus_tag="ACF3T5_001814"
     CDS             complement(180..737)
                     /gene="frr"
                     /locus_tag="ACF3T5_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFD3632415.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(740..1462)
                     /gene="pyrH"
                     /locus_tag="ACF3T5_001815"
     CDS             complement(740..1462)
                     /gene="pyrH"
                     /locus_tag="ACF3T5_001815"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFD3632416.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1598..2479)
                     /gene="tsf"
                     /locus_tag="ACF3T5_001816"
     CDS             complement(1598..2479)
                     /gene="tsf"
                     /locus_tag="ACF3T5_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFD3632417.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="ACF3T5_001817"
     CDS             complement(2578..3360)
                     /gene="rpsB"
                     /locus_tag="ACF3T5_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFD3632418.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3719..4198
                     /locus_tag="ACF3T5_001818"
     CDS             3719..4198
                     /locus_tag="ACF3T5_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFD3632419.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4415..5506
                     /gene="phnW"
                     /locus_tag="ACF3T5_001819"
     CDS             4415..5506
                     /gene="phnW"
                     /locus_tag="ACF3T5_001819"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HFD3632420.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKELLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5499..5627
                     /locus_tag="ACF3T5_001820"
     CDS             5499..5627
                     /locus_tag="ACF3T5_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFD3632421.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5631..6176
                     /locus_tag="ACF3T5_001821"
     CDS             5631..6176
                     /locus_tag="ACF3T5_001821"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HFD3632422.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6630..8321)
                     /gene="argS"
                     /locus_tag="ACF3T5_001822"
     CDS             complement(6630..8321)
                     /gene="argS"
                     /locus_tag="ACF3T5_001822"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFD3632423.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8741..9688)
                     /gene="arcC"
                     /locus_tag="ACF3T5_001823"
     CDS             complement(8741..9688)
                     /gene="arcC"
                     /locus_tag="ACF3T5_001823"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFD3632424.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9803..10822)
                     /gene="argF"
                     /locus_tag="ACF3T5_001824"
     CDS             complement(9803..10822)
                     /gene="argF"
                     /locus_tag="ACF3T5_001824"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFD3632425.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10913..12142)
                     /gene="arcA"
                     /locus_tag="ACF3T5_001825"
     CDS             complement(10913..12142)
                     /gene="arcA"
                     /locus_tag="ACF3T5_001825"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFD3632426.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3389 a         2548 c         2214 g         4193 t
ORIGIN      
        1 acttaaaaga ttcccactat cttaagtatt atagcaatat ctaactgttt ttcctatgaa
       61 atcattgttt ttttatttaa aaactgaata attttttctt tttttaattt ttaaataaaa
      121 gaaatataaa taaaaacaga ggaagcggaa tgatccgccc ctctgttttt atgattttat
      181 taaacttcca ataattcttt ttctttatca gcagcgatct tatctacttc tttgatgctg
      241 ttatctgtca atgtttgtac gtctttttct aatccgcgca aatcatcttc ggtaatatca
      301 ccattttttt gttgtttttt atattcatcg atcgcatcac gacggatgtt acgaatcgcg
      361 atttttgcat tttctgcttc ttttttaaca tctttagcta attcttttct gcgttcttct
      421 gtcagttgcg ggataactaa gcgaatgaca tttccatcat tggttggact gattccgata
      481 tcacttgcca taatagcttt ttcaacatct tgaagaatgc tcttgtcaaa aggtgtgatc
      541 attaaaacac gtgcttctgg aatttggatt gatgccattt ggttcaatgg tgttggcgca
      601 ccgtagtagt tcacagtaat acggtccaaa aggctagcgt tcgcacggcc tgcgcggatt
      661 tgcccaagtt cacgttgaag attttgtgct gcttttttca ttttttcttt tgcttctgtc
      721 ataattgcat ctgccatttt tatttccccc ttacagttgt tccgatattt tcgcctaaga
      781 ttgcacggcg aatatttccg tgttcattta aattgaagac aagcagtgga atatcattat
      841 ccatgcttag tgaacttgca gttgaatcca tgacttgtaa tcctttggca ataacttcca
      901 gatgagtcag ttcttcaaat tttactgcat ttgcatctac tttcggatca gcagaataaa
      961 caccgtctac attgttttta gccatcagga taacatctgc gccgatttct gccgcgcgta
     1021 atgctgctgt tgtgtctgtc gagaagtacg ggttgccggt accgcctgca aagataacga
     1081 tccgtccttt ttctaaatga cgttctgctt tacggcgaat atatggctct gcgatttgac
     1141 gcatttcaat cgaagtttgc acgcgtgtcg gtacacccac gttttccaac gtatcctgta
     1201 aagctaaggc attcataact gtagctaaca tacccatgta atcagcttga gcacgttcca
     1261 tgcccatttg tgctccgatt tgtccgcgcc agatatttcc gccgcccaca acgattgcca
     1321 tttcaacgcc cagctcgtga acttctttga tttcttggat gatttctttg attactggtg
     1381 gtttgattcc aaatcctgcg tccccagcta aagcttctcc actcaatttt aagactacac
     1441 gctgatattt cggtgtgacc atgttcattc ctccaatatt ttctattgcc attttaccat
     1501 acttagatga tttcgacact ctaagactgg tttttcttgt tttgcttaaa aaaagggagc
     1561 gcacaaaccg tgcgttcccg tctgttattc aacagaatta ttttttcact tggctcataa
     1621 cttcttctac aaagttatct tcgcgttttt cgataccttc gccaacttcg aaacgtacga
     1681 atgttttaac agttgcgcct tttgatgcaa cgtatttttc aacagtcata tcaggatctt
     1741 taacgaatgg ttggtccacc aaagcgattt ctgctttgaa tttcttcaag cgtccttcta
     1801 ccattttttc aacgatattc gctggtttgc cttcgttcaa tgcttgttct gtaagaacag
     1861 ttttttcatg ttctaattca gcttctggaa tttgtgattc gtttacataa cgaggattga
     1921 tagctgctac gtgcatagct acgtctcttg caactgtttc gtcagttgtt ccatctagaa
     1981 cagtcaatac ggcaatacgt ccgcccatat gcaagtatcc accaaatgca gcattgtcgt
     2041 ctttttctac aacttcaaag cgacggaagc tgattttttc tccgataact tgagttgctt
     2101 cgatcaagtc agattcaatt gtacctttat ctgttttgat tttcattgca gcttccatgt
     2161 ctgctggttt gttttcagca acaagttctg caatttcttt tactaaatct tggaacattt
     2221 cgtttttaga aacaaagtct gtttctgagt ttacttcaac gattgctgca gtgttacctt
     2281 tcacagcaac tgaagccaat ccttcagctg caatgcggtc atttttcttc gcagcctttg
     2341 ccattccttt ttcacgtaaa agatcaactg ctttttccat gtcgccttct acttcaacta
     2401 atgctttttt cgcatccatc attcctacgc cagtcatttc gcgtagttct tttaccattt
     2461 tagctgtaac gtctgccatg tttaactcct cctgtgtgtt tttcttttta aaaaaagctg
     2521 cttcaaagga gagtgaggct tgtcgcccag cctttgaaac agctccatca ttcaaaatta
     2581 ttctgctgaa gaattgtcgc cttcaacaac atctacgatt tcttcgattg atgttgctgt
     2641 gttttcttct gtgaaatctt cttcaactac ttgatcttca ccttggtttc cttcgatgaa
     2701 agcatcagcc atttttgaag taatcaattt aacggcacga attgcatcgt cgtttgaagg
     2761 gattactacg tcgatttcat ctggatcgca gtttgtatca accatagcta cgattgggat
     2821 atttaatttg tgtgcttctt gaacagcaat acgttcttta cgtgggtcaa cgatgtacat
     2881 tacatctggg attcttggca tatcagcgat accgcctaag aatttttcaa gacgttcacg
     2941 ttctttgttc aaaccagcaa cttctttttt aggtagaact tcaaaagttc catcttcttc
     3001 cattgcattg atttgtttca aacggctgat acgtttttgg atagtatccc agtttgtcaa
     3061 tgttccgcct aaccaacgat ggttaacgaa gtattgtcct gcacgagttg cttcttcttt
     3121 gattgcttct tgtgcttgtt tttttgtacc tacgaataat gctacgccgc cttcttcagc
     3181 aacgctcttc atgtaatcat aagctgcatc tactaattta actgtttttt gcaagtcaat
     3241 gatgtagatt ccgtttcttt ctgtgaagat atatttcttc atttttggat tccagcgacg
     3301 agtttggtga ccaaagtgta cgccggcttc tagtaattgt ttcatagaaa ttactgccat
     3361 gtttgtttcc tccgatttgg ttttattttt cctcttctta tcttctacct tgccggcaaa
     3421 ctttttcaag cacctaccga caatccaata agaatgtgga tttgctgatg cataaagcac
     3481 cctcagctat tatacagaca aaagatccgt atcgcaagag caaatatact tttctgctca
     3541 acattcaata gtttacctga aagataaaaa gaattcaaga aaaacagtag atttctcttt
     3601 gtttctttat tatccttttt ccataaaata attttaggta taaaagacga atattattgg
     3661 gcaatccttc ttttttttgt tatgatacat gtgaaatctt tcgtaaagga gaaagaaaat
     3721 gcgcaaagct caaagacatt tgctgattaa acaagtcatt gaagaattcc cgatccgtac
     3781 gcaagaagaa ctattaaaca aacttgggca ttatggcgtt accgccacgc aggcaacgat
     3841 ttctcgagat atccgtgatc taaaaatcgt gaaggcacct gatgaaaatg gcgtttcccg
     3901 cttcgtattg tttcgcgaaa aaggaactcc tgagactagg actgaggaag aggatcggct
     3961 ggttcaaatg attgaagaca ttgtattgaa agtcgaacga gtccactttt tgacgatcgt
     4021 caataccttg ccagacaatg ctcatttatt tgcatctgtt ttagatgaga tcaaacctcc
     4081 ctctattgtc agtacaattg ctggattcga tacgatcatg gtcatctcgg aaaatgagga
     4141 ggacgcaaaa cgagtagaag aatttcttca ccatccaact gaagtatcac ttttttaatc
     4201 agagcacgta ttcctaatat ggaatgcgtg tttttttatt ttcaatattt tccttattga
     4261 ataaaccttt acactcttta caaaaataca tcatttttta ttcaaatttt tcaacacctt
     4321 gattgaatca caataatcat catttatatt ggatgttaac aagagcagct tcttgctctt
     4381 tgtgtatttc aaaaaagaaa tgaaaggaaa tcatatgtat aaattattaa cttctggacc
     4441 attgacgact tctgaaacag tgaaaaatgc tatgctgatc gatcattgta cttgggatca
     4501 agaatacaaa gaaattacac aatggataag aaaagaatta ttggatcttg ctcaagtatc
     4561 ggaaggaata tacacaacgg tcttaatgca aggtagcagc tccttcgggg tagaatcggt
     4621 attaagttct gtgatcaggc cagaagatac tgtactgatt tgttcaaacg gcgcatatag
     4681 acaacgaatg atcaacatgt gcgaatgtct gaaactgaac tataccgttt atgaggtatc
     4741 ggaacatcaa atcctccaag ctcatgtgat tacacagctg attgaagaga atccgtctat
     4801 ctccgcacta gcaatggtcc atagcgagac tacttccggt attttgaata atttggaaga
     4861 gattgctgct gtcacgcaag aaagagatct tttgtttata gtggacgcga tgtcaagctt
     4921 tggtgggata ccgatttgtg tagccgaact agggattgat tttttgattt ccagctcaaa
     4981 caaatgtatt caaggtgttc caggcttttc ttttgtactt gctaataaag cggaactgga
     5041 aaaacgaaaa aatgttagcc gaagtttgtc acttgatcta tatgaccagt accgcgtgat
     5101 ggaaaacgag aatggcaaat ggcgtttcac ttcccctacc catacggttt tggcttttca
     5161 acaagcgctg aaggaactag aggcagaagg aggaatcgaa actcgatatt cacgttatct
     5221 cgccaataat catgtgctac gacaaaaaat gacagagatg gggttccagc ctttcattca
     5281 agaatatcag gggccattca tcacctcttt tctttaccct aaggactggc gatttgattt
     5341 taatcatttt tatcagtatt taaagcaaaa tggatttgtg atctatcccg ggaaaatcag
     5401 cgatcatgaa tgttttcgta tcggaaatat tggcgagatc tatccggaag atattttgga
     5461 actgacagca attatcaaag actataagga gaacgttaat gattaaaact gtaatttttg
     5521 attgggcagg aacaactgtg gactttggct gtatggctcc tgtccacgct tttagaaatg
     5581 cttttctaga aaaaggaatc caactgactg acaaagaaat ccgctaaccg atgggaaaat
     5641 taaaatggga tcatatccaa cagcttcttt ccctcccttc tgtcaaaaaa caatggatag
     5701 ctatttatgg acaactacct caggaaaaag aggtggagga attgtatcaa caatttgaac
     5761 attatctgtt taaagatttg gtcaagcatt ctaccttgaa acccgacaca ttggaaacga
     5821 tcgaacggtt gagaaacgct gggatcaata ttggttccac gaatggattt aatgctccta
     5881 tgatgacaat cgtagcagaa accgcaaaaa aagaaaggta ttcaccagac ttcatggcaa
     5941 cttttgaaga cgtcgaaggg tatggtcgac cgtttcctta tatgatccat aaaaacatgc
     6001 agtacttcaa aaacaagtcg gttgatgaag taatcaaagt cggagatacg gtctcagata
     6061 tccaggaagg gaaaaatgcc ggagtgttga cagtaggtgt aattgaaggt agttcgctaa
     6121 tggggatttc ttatgatgag taccaaaaat tagagatggg gaaaaagatc gtttaaaaca
     6181 gtctgttgct cttagattcc aacaagccgg cgctgatatt tgtgtaaaca atttaaaaga
     6241 gttaacagaa tacattttgt acgctgacat gaatccagaa tgacttttat acaaaataag
     6301 aggttgtgaa aaagctgtcc tgtatcaagt aataaaaaca gccaagcaaa aatagctcct
     6361 catatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6421 cgaaaaaaaa aagggagctg tgacacgact tttgtcacag tccccttatt tgcaaacaaa
     6481 cagtgtttaa gagacaacct ctccactaaa cggtgttcat tcagctgact ctcggtattt
     6541 gctcaaaaaa cgataaaatt tgaaaagcta ttttctcttt tgtttctcta atactcggat
     6601 cagaacgctg atttcacatt ctctttttct tacatcttat ctggtgcatg aagtcctagc
     6661 aaacgaaggt cttctttcaa taatacagtc actgcataaa ctagagctaa gcgtgcttct
     6721 ttttgttcat catcagctaa aatcttcgta tgagcatagt atttgttgaa tgcttgtgct
     6781 aatttgattg catgtttcgc aatcactgac ggttcgtatt tttccccagc agacaagacc
     6841 gtttcagggt atttttgaac taatttaacc acttcccagc tggtatcgtc attcaatgca
     6901 tagtctgctg tttctgaagg gacaaatcct gctttttcca acaggctgac tgcacgagca
     6961 tgtgtatatt gcacataagg tccagtttct ccttcaaatc gaacaacttc ttccaggtta
     7021 aagtcaaatg tattcagtcg atcgtttttc aaatcgtgga aaatgactgc cccaacccca
     7081 acttgtttag ctacagcatc tttgttttcc aaatcagggt ttttctcgct gatttgttct
     7141 ttagcagatt ggattgcttc atttaatact tcttctaata agacgatctt tcctttacga
     7201 gttgataatt ttttaccacc ttgcgtgatc aaaccaaatg gaatatggtg catgtcgtca
     7261 gaccagtcaa agcccatttc cttcaagaca gctttcaact gtttaaaatg atagctttgt
     7321 tcattcccta ctacatatag agattgtttg aagtcgtaag tacgtttacg gtacaatgca
     7381 gcagctaagt cacgtgtaat gtatagtgtc gcaccgtctg acttcttaat caacgctgga
     7441 ttcaggtcat aagcagatag gtcaacgatc tctgcacctt tatcctcgtt cagcaaatgt
     7501 ttttcttcta aaagtttgac gatttcatcc atcttgtcat tgtagaaagc ttcaccgttc
     7561 aacgaatcga aacgtacttc cagcagatca tagattttat tgaattcttt catcgattca
     7621 tcacggaacc attgccataa ttgaatagcc tctttatcgc cttcttccag acgtttgaac
     7681 caagcacgag cttcttcatt taattctggt tctgtttcag ctacttcatg gaattggacg
     7741 tataaacgaa gcaattcatt gattggttct gctttaacag cttcttctgt accccatttt
     7801 ttgtaagcga cgatcagttt tccaaactgt gttccccagt cacctaaatg gttgatgcgg
     7861 attggctgat aaccgatttt ttccataatg aaaccgatcg aattaccgat aactgtcgaa
     7921 cgcaaatgtc ccattgagat tggtttggca atattaggag aagacatgtc gattggtact
     7981 gttccttgat tcccgatgtt gctatcccca taatgttctt tttctttcac tactgtttgc
     8041 aaaacttttt tgctgataag ttctttattc atgaaaaagt tcaagtaagg tccgacaact
     8101 tcgatttttt caaagttggc agaatcgatt ttttctgcta aatctgcagc gatttgctgt
     8161 ggcgcttttc ggtaaacttt tgctaatgaa aaggctggaa atgcaacatc tccgtgttca
     8221 gctgattttg gattttctag taattgctcg acttgttcca gtgttaaatc atctttgaca
     8281 gcgtcataga cagcttttgc gacaagatct ttattattca tctttattcc tcctaaagtg
     8341 ttcccttgat tgttaaacaa aaaaaatctc tagcgacggc atacttagta tgccatcact
     8401 agagacgaga tttcccgcgg taccactcca attgtcctaa aaaaggacca gctccatctg
     8461 atatcggtag acgaccgtct gtttaaaaca gaattctcca agtgcgcttc gttgtatctt
     8521 cttcattcgg cttccaccac ttccgaattc gctagccaag aatgatacaa gtactctctt
     8581 gttcaacaaa tcttagtctt attaaacttt cttgaattat atcagaaaaa aaaacgaaag
     8641 gctagtcgtt tcataaaaaa acaatccaga tggcctaaag gagaagaaat accacctgga
     8701 tagctataaa tcatttcgta aatacgaaag acttaacaaa ttaatcagct acgataatcg
     8761 ttccttcttc agaagctaaa agattctcga tattttctaa agaagtgatg attgccttag
     8821 cgtttggttt tgcttcaaca aatgcaatag ctgcttctac tttcggaagc atgcttcctg
     8881 gagcgaattg cttttcatca atgtattgtt tcatttctga aacagtaaca gtttctaatt
     8941 tcttttgatc tggtttttgg taattgacat acacattgtc tactcctgtc aatacgatca
     9001 gcaaatccgc atcgatgatt tcggctaatt tttctgaagc aaaatcttta tcgattacgg
     9061 cttctcgtcc ttctaatcca gtagctgttt ctacaacagg aatcccaccg ccaccaacag
     9121 agactgtgat aactccttga tcgaccagcg tttcgatgac acgtgcttct ttaatcgaga
     9181 tgggtttagg ggaagcgact acttttctcc atccacgtcc agcatcttct ttgaatgtca
     9241 cagtggagtc agcattcatt tgttctttgg cttcttcttc cgtgtagaat ggaccgactg
     9301 gtttacttgg atttttgaat gaaggatcat tttcatccac aataacttga gtgactaaag
     9361 aaaccacatc tttatcgatc cctttttcct tcagtacttc gcccatggcg ttttgtaacc
     9421 agtaaccgat agatccttct gtcattgcaa cacatgtatc taaaggcatt gccggtgttt
     9481 tctcagaatc agcagcttgc tgctgaatca ataggttacc gacttgcgga ccatttccgt
     9541 gggagatgat caattcatct ccttgttcga taaatttcac taaatacttt gctgtttcca
     9601 ttaacgcttc ttgttgggct tttgcacttg catccgtaga caagatggca ttgcccccta
     9661 aagcgacaac tacttttcgt ttggccattt cattcgtcct ttcttcagtt ctaccgaatt
     9721 ttctagataa caaaacgagc cagcgttttg tgattttccc tcaatgctga ctcgtttttt
     9781 cgtgagatga atgtggttat gcttaaacgc gagggataaa caagttacct aatgttgctg
     9841 ccatgatcgc tttgattgag tgcatacgat tttctgcttg gtcaaattga cgagcatatt
     9901 tgctgcggaa gacttcatct gttacttcca tttcagtgat gccaaagcgt tctttcattt
     9961 gttcaccgta aacagtgttt gtatcatgga aagctggcaa gcagtgcaag aagatcaaac
    10021 gatctgtgtt gtgtgttttt tctaccattt ccatattgat ttggtaaggt ttcaacaatt
    10081 tgatacgttc ttcgaattta tcttcttcac ccattgatac ccatacatca gaataaagga
    10141 cgtctgctcc gtctacacct ttatcaacat cgtcggtaat catcaattgt gcaccggatt
    10201 tttcagcaaa gccttctgcc attttgacga tttcttcttc aggttgtaat tcttttggtg
    10261 caacgatacg catattcgct cctaaaattg cacctgtgac taataaagag ttagccatat
    10321 tgtttcgacc atctccgcag taagcaacag tgataccttc tactgttcca aagttttctt
    10381 ggatagttaa aaagtcagcg atcatttgtg ttggatgcca ttcatcagtc aagccattcc
    10441 aaactggaac accagagaat tctgctagtt cctctaccat tttttgactg aatccacgga
    10501 attcgattcc gtcaaacata cgtcctagta ctttagctgt atcttctgtg gattcttttt
    10561 tacctaattg gatatcattt gcacctagat attcaggatg tgcacctaga tcgatcgctg
    10621 cagtagtaaa tgcagaacga gtacgtgtag atgttttttc aaataaaagc gcaatatttt
    10681 ttccttctag atagtgatgt gggataccac gttttttcaa gtctttcaag tgttctgaaa
    10741 aatcgatcaa atattgtaat tcttcttttg taaagtcttt ttctgctaat aagcttcttc
    10801 cttggaatac tgattctttc atattaaaca ttctccttta gattgttttt atttaagatt
    10861 aaagaggaaa tcccgacggc agctgacagt aagactgccg tcaggaaaca aattacttca
    10921 agtcttcacg gacaagtggt tggctcatgc agcgtggacc accacgacct cgtgacaatt
    10981 cacttgaatt gatttctaac actttgatgc catagctacg aagcaattcg ttcgatacat
    11041 aatttcggtt ataagtgact acaacacctg gagcgattgc caatgtgttt gatccatcat
    11101 tccattgttc tcgaggtgca acgatctcat ctccattacc agttgggatc agtacaagat
    11161 catctaatcc aagagcagct ttcatcgttt cgtgtaagtt atctgaatga gtaatcttga
    11221 tatcatcacc atctggttcg attgtaaaga catccatctt accgtcttta ctttgaattg
    11281 ctggatggat agtgaattta tcgtgatcta ccattgtaaa tacggtatcc aaatgcatca
    11341 ttgcacggtt gttcggaatt ttgattgcca acactttttc aaatcctgaa ttttttgcga
    11401 ataggtggcg tgctaatttt tctaaagctt tcgcattagt tcgttgagaa atcccaacag
    11461 ctactacttt atcgcttaag atcaattcgt ctccgccttc gatatgatct ggattttcac
    11521 gatccaacca tacttcgatt cctttatttg caaatcttgg atgatgtttc aaaatatatt
    11581 ctgtaaacat cgattcacga cgacgtgctt caaaagtcat cttattgact gtcatcccat
    11641 ttcccattga tgcggaaggg tcgcgtgtaa agtacaagtt tggcattggg tccatgtaaa
    11701 atggatagtc atcgtctgct gacagatcat atagactgct agaacgtact tcgatatctt
    11761 ttgtacgaac cccagccatg attttatcta ccatttcttg cgtttccatt gatagaagga
    11821 actcatgaag accctctctt acagcattcg atgcaatatg agattcatcc aacattttat
    11881 tcaagaattg ttctttgaca ttgcctgcgt caatcgcttc tgccgctaat ttttccaagt
    11941 aaagagtctc gacgccttca ttttgcaatg tcttcgcaaa gttgtcatgt tcttcttgag
    12001 caatcggaag atatggaata tcatcgaaca acagacgatc cataatatct ggtgtcaaat
    12061 tttccacttc ttgtccaggc cgtttcagta aaactgtctt taatttccct atttcagaga
    12121 aaacgtgaat aggtttatcc atgttgtgtt gcctccttct ttttgattac atgtatagga
    12181 tatcctcctt ttgtaatcac tttcataatt ttgtctcatt caaaacgaga cattcctcac
    12241 attaatattc gataattaaa taattaaacg tttttctatt tttaagcgta atttattcat
    12301 tttttattct gtatacactt tcaaaaaaat atttattcat tgaa
//
LOCUS       DAXLPI010000073        12171 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXLPI010000073 DAXLPI010000000
VERSION     DAXLPI010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.073"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(133..297)
                     /locus_tag="ACF3T5_001826"
     CDS             complement(133..297)
                     /locus_tag="ACF3T5_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632427.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="ACF3T5_001827"
     CDS             592..1920
                     /locus_tag="ACF3T5_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFD3632428.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="ACF3T5_001828"
     CDS             2122..2955
                     /locus_tag="ACF3T5_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFD3632429.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="ACF3T5_001829"
     CDS             2971..3708
                     /locus_tag="ACF3T5_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFD3632430.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="ACF3T5_001830"
     CDS             complement(3863..4831)
                     /locus_tag="ACF3T5_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFD3632431.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="ACF3T5_001831"
     CDS             5063..5896
                     /locus_tag="ACF3T5_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFD3632432.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="ACF3T5_001832"
     CDS             5930..6769
                     /locus_tag="ACF3T5_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFD3632433.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="ACF3T5_001833"
     CDS             6795..7355
                     /locus_tag="ACF3T5_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3632434.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="ACF3T5_001834"
     CDS             7554..9275
                     /locus_tag="ACF3T5_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFD3632435.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="ACF3T5_001835"
     CDS             9440..10582
                     /locus_tag="ACF3T5_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFD3632436.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="ACF3T5_001836"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="ACF3T5_001836"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFD3632437.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4147 a         2042 c         2528 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt ggataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DAXLPI010000074        12136 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXLPI010000074 DAXLPI010000000
VERSION     DAXLPI010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.074"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..610
                     /locus_tag="ACF3T5_001837"
     CDS             <1..610
                     /locus_tag="ACF3T5_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD3632438.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="ACF3T5_001838"
     CDS             723..1001
                     /locus_tag="ACF3T5_001838"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3632439.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="ACF3T5_001839"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="ACF3T5_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFD3632440.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="ACF3T5_001840"
     CDS             1458..2213
                     /locus_tag="ACF3T5_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD3632441.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="ACF3T5_001841"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="ACF3T5_001841"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFD3632442.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="ACF3T5_001842"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="ACF3T5_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFD3632443.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="ACF3T5_001843"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="ACF3T5_001843"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFD3632444.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="ACF3T5_001844"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="ACF3T5_001844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFD3632445.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="ACF3T5_001845"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="ACF3T5_001845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFD3632446.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="ACF3T5_001846"
     CDS             6950..7492
                     /locus_tag="ACF3T5_001846"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFD3632447.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /gene="atpA"
                     /locus_tag="ACF3T5_001847"
     CDS             7515..9071
                     /gene="atpA"
                     /locus_tag="ACF3T5_001847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFD3632448.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="ACF3T5_001848"
     CDS             9085..9987
                     /locus_tag="ACF3T5_001848"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFD3632449.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="ACF3T5_001849"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="ACF3T5_001849"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFD3632450.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="ACF3T5_001850"
     CDS             11430..11852
                     /locus_tag="ACF3T5_001850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFD3632451.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4036 a         2155 c         2676 g         3269 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcactaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttattt
//
LOCUS       DAXLPI010000075        12127 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXLPI010000075 DAXLPI010000000
VERSION     DAXLPI010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.075"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..884
                     /locus_tag="ACF3T5_001851"
     CDS             <1..884
                     /locus_tag="ACF3T5_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFD3632452.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            932..3061
                     /locus_tag="ACF3T5_001852"
     CDS             932..3061
                     /locus_tag="ACF3T5_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFD3632453.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3036..3725
                     /locus_tag="ACF3T5_001853"
     CDS             3036..3725
                     /locus_tag="ACF3T5_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632454.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4133..4819)
                     /locus_tag="ACF3T5_001854"
     CDS             complement(4133..4819)
                     /locus_tag="ACF3T5_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3632455.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4955..5149)
                     /locus_tag="ACF3T5_001855"
     CDS             complement(4955..5149)
                     /locus_tag="ACF3T5_001855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632456.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5199..5639)
                     /locus_tag="ACF3T5_001856"
     CDS             complement(5199..5639)
                     /locus_tag="ACF3T5_001856"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFD3632457.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5643..6488)
                     /locus_tag="ACF3T5_001857"
     CDS             complement(5643..6488)
                     /locus_tag="ACF3T5_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFD3632458.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6637..8055
                     /gene="pepV"
                     /locus_tag="ACF3T5_001858"
     CDS             6637..8055
                     /gene="pepV"
                     /locus_tag="ACF3T5_001858"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFD3632459.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8131..9333)
                     /locus_tag="ACF3T5_001859"
     CDS             complement(8131..9333)
                     /locus_tag="ACF3T5_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632460.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(9320..11107)
                     /locus_tag="ACF3T5_001860"
     CDS             complement(9320..11107)
                     /locus_tag="ACF3T5_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HFD3632461.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(11120..12073)
                     /locus_tag="ACF3T5_001861"
     CDS             complement(11120..12073)
                     /locus_tag="ACF3T5_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD3632462.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
BASE COUNT         3931 a         2241 c         2407 g         3548 t
ORIGIN      
        1 gtatttcagt accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg
       61 ctaatatcgt caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg
      121 aaagctatac ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag
      181 ttgtggtact aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag
      241 caaacgtacc agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac
      301 cagttttagg ggtcgttctt ttagatcttc aaatcatctt aggttttatt ctagcaggta
      361 tctgggcatt gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt
      421 ttgcgaaatt atttacagat ggcgcagtcg atgttgcacc aatggttggt ttcttgatga
      481 ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg
      541 gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac
      601 taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg
      661 tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt gcaattacaa
      721 cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata
      781 caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggtg
      841 cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat
      901 aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga
      961 tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg
     1021 aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag gtgtcgttca
     1081 gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg tgtttgttgc
     1141 acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt
     1201 aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa
     1261 cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa
     1321 aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga
     1381 ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta
     1441 tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca caaaattata
     1501 tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac caaaaatgct
     1561 agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg tttctttgat
     1621 gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt
     1681 aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact tgagagcgtc
     1741 taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg
     1801 acgtccagca atcgattatt caattgtagg tggccaccca acatatatct cttcattaga
     1861 tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat
     1921 cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac
     1981 agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga
     2041 ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc
     2101 caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg
     2161 aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca tcgcagagca
     2221 gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta
     2281 tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat
     2341 tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat
     2401 ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc
     2461 gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc
     2521 aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata cttcaaaagt
     2581 aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac
     2641 aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg
     2701 cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc
     2761 tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc
     2821 atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat
     2881 ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag
     2941 agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt
     3001 atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata
     3061 agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc
     3121 agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac
     3181 aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg
     3241 ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc
     3301 aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc cgattgatga
     3361 cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc
     3421 aagctttgat tattggacat gaactatttc accacatcga agcttctgtt aagggaattt
     3481 atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca
     3541 ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt
     3601 tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag
     3661 ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat ttagattaca
     3721 aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacggtgttc
     3781 tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg
     3841 aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaaaggtgt
     3901 tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag aagctatttt
     3961 tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg
     4021 tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat
     4081 tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga
     4141 tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct
     4201 cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat
     4261 caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg
     4321 aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt tctagccaat
     4381 cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat
     4441 caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct agtatttttt
     4501 ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt
     4561 ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat
     4621 aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt
     4681 ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac
     4741 ttccaggtgc taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt
     4801 gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta ttataacata
     4861 aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca
     4921 tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg
     4981 gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa
     5041 tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt
     5101 gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta
     5161 ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa
     5221 gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag cttcactaat
     5281 cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg
     5341 tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata cttttggata
     5401 gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact
     5461 tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt
     5521 accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc
     5581 cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg
     5641 actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt cggaagtgct
     5701 tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa
     5761 ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat
     5821 cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc agcagaacct
     5881 aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg
     5941 agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc
     6001 ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga
     6061 tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc aatcaccagc
     6121 cattgttctt ttgattgttt ttctagtact tgtttgaaac gttccaaact cttagaagaa
     6181 gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg
     6241 tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag agcactccga
     6301 tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc agctaaagca
     6361 ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat
     6421 gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag ttgttcgttt
     6481 atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca
     6541 gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat
     6601 gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa
     6661 gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc
     6721 gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca
     6781 ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac
     6841 tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg
     6901 gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat
     6961 ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat
     7021 gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc
     7081 ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa
     7141 ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat
     7201 gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt
     7261 gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca
     7321 agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca
     7381 gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc
     7441 gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta
     7501 gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc
     7561 catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa
     7621 ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc
     7681 atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc
     7741 gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca
     7801 cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac
     7861 actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa
     7921 cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac
     7981 gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat
     8041 cgtttggcga aataaaaata ctaaaaaact ccggctatct ctgacagctg gagtttttat
     8101 gtttaaaaat gaattagtac atcggtattc ttaaaagtaa tttagttcgt cttttgattt
     8161 ctcaggaggg atcaaaaagg cgaaagcact aagtatagca aaacttgtga tgatttgcgt
     8221 acgatacgta aaccaataat gaatctgtga gtgattagct aaaatattgt accaaatata
     8281 cggcgttaca gcagctaaaa caaacaaaac tagaagttgg aacggacgcc agctatttcg
     8341 tttacgataa gcaaaataga gaaaaacaag acaagtaatc aatagcaata atattgctac
     8401 ttttggatac atcaatctaa tattgctttt cagcatctct aatcgatgca acgggtattt
     8461 gtcgtttcct tccgttcgaa agaggatttg gttgattgca tcttttacaa tgtttttgtt
     8521 caatatgatc gatgatagga tccatttagc cacccatgtc aaaccatagc ctaatcccca
     8581 aaagataccg gtaaaaacaa aactgcctac ttgtttgctc aatgaactgc gtttatccat
     8641 taaatactta tttgttacat aataaataat gatcacaggt atcccccaag agatgaccgg
     8701 tgttgtcaac aagtcaaaaa agttagctaa agaaccaatg acaaagaaat acaagaataa
     8761 ttgttggttg gtgttggaga gttgtggttt tgttaaaaat agtaatacag acaataaagt
     8821 caaaatccaa atatttgaaa attgcatact gaagaaaaag gttgcagcat ttcctatagc
     8881 aagcgaaatc aacaaggctg tcgttaaata gatgtcgcta cacttggtta aaaagtagct
     8941 cgtcaatcca atcagcagaa aaagaatggc tgcgtaaatc attcgaatac tccccaaact
     9001 catccctagc agtaaaggtc gtaaaaaaag ctgataacca tgccaataac ggggataatc
     9061 cgccatagac atcgcgttct ccaaggagtt caattcagga tctgcttttg tttttcgtat
     9121 catcaaatga tcagtaaaat tatctaaccg agtgcctagc atatttcctt gattgataga
     9181 aggatacatt ccttcttttt caaccatctg tagagatttt tctgcattcg attcaataga
     9241 gtgctgcgga atgaaaaaag taattgtcag tagtacgcaa tagctagcga tcaagagaac
     9301 aaaagcaaaa aatatctttt taaatagttt catgagtatc tcctatatct gttttcattt
     9361 tttctctttt atctagtaaa aaaggagaga ggatataaac aggtagacct aatgctaaaa
     9421 caaacacata ccgtaaacta aaagccacag gagtagcaat cataatagta gcaaaaacaa
     9481 gcataccggg aagaaaaagg agtaaatacc gatatttttt cttaaagatt gctaaaagtg
     9541 cactgccgat agctaaccac agtaatgtgc cggaaccaaa gaaatcacga gcatcgatga
     9601 tttttttcat tcctgacaat ccagtaagat gttcaatcaa atctacttgc ttcatcccat
     9661 aattattcgt gtttacacga gtatctaaaa agccgtaact attttttgta ccaggtaccc
     9721 agaacccgaa cgtttctaaa atataagcat ctgtatagat ttttatatta cttggtaaat
     9781 ttcttgccca tagtgataga aattcttttt tatgtgtatt caaaaactca ttgttaaaat
     9841 ttttatgcca cttgattgag tctgcaagac taggagaata cgcttcataa tcttttatcg
     9901 ggagtagctg attcatgaat gctttatctt tcttagacaa gtcaccatcc ataacaatag
     9961 ttctagaaat ttgctgcaaa ggaacaccta attgttcagc agtttctgta ggaatagcat
    10021 aacgagccat caacgggtgg atgagtagtc cagcaatcac tgtataaccc agcaccactt
    10081 gaaagaatgc ttgatacctt ttttgtttca aaataatcaa gccgcaaatc aaacctgtgc
    10141 cggttactac ataaatccca ttatttctta gataggtggt cagtaaagca aggatgatta
    10201 cgtaaatctg attgagaatc gttggctttt ctcgttgaaa aataaagtca aaaaccgcta
    10261 aggaaaataa aaagcaagcg atagagaaca aagtatcttt ttgcatgttc aaggcgtaga
    10321 ttggaaaaac cggtgcaaat gcaaaatagc ataaactaat gagtaccgga aaaaaatgga
    10381 tgtttctttt taacagccag caaagagaat aacttatcgc tgaagctgtt atcaatgttt
    10441 gaaaaatcga aaataaaaat acaccgtgat tgatcgtatc agttggtaaa tcatgaacaa
    10501 atagcttgac cagtaaggtg aataagactg gatgatgatt gcttatagga acatcaccca
    10561 tagattgggc gaccgaactt aacgaatcag gcaaaaccac tccaggatac aatgccaaca
    10621 gataaggaag ccaacacaga actaaaaaga cagtaaagaa taaaaaatat tttcttgaag
    10681 agaaagcctg tgtgttttga gcagtaaaaa atgtttccag ttttggcatt gataaataaa
    10741 tcgattgtaa aaataaaaaa gaatacacaa aaatggagaa ataaacaaga aagtaaaaaa
    10801 tacttccatc agcaatcgtt gtctttgtat agtttgctga gacatactga aattgtatct
    10861 gtctgccaat agaaagtgta agagcaaaag ctgcagaaaa aaacaatcgg gcaatagctg
    10921 caactttgtt ttttattgaa aacaactcta ttccatattg cttaaaagca agtagcaaaa
    10981 ccatggctaa accaatgatt actgttggat tcaaagttcc tagaccataa gaagtaaaaa
    11041 aagcaaaacc tgacgcaaat aaagttgcaa aaaaagtagg aaatagattc tcctttttct
    11101 tatgcataaa aaaggcctcc tacttcattt ctttattttg atattcgtgg tagacatggt
    11161 ataaattcaa ttcatattct ttcttggcat ttgtgacgac cgtatccaaa atcaaccctg
    11221 ttactaatga aagaagggct aaagtcatca atccagtact taaaatcgca gaagggatgc
    11281 gtgtaataaa gcttgtcagc atatattcac gaatcacagg aatccccatg accaatccaa
    11341 ataaaaagaa gaagatggtc cagatcccaa agaaaagcaa aggtttataa tctttgcaca
    11401 ttttgatgat catcatgatc actttgaatc cgtcagagaa tgtattcaat tttgattcgc
    11461 taccttcagg acgatcgcga taatcgattg gaatctcaac gaacttgaat tttttatcta
    11521 aagcatgaat cgtcaattct gtttcgattt gaaaaccaga actcataata gggaagcttt
    11581 tgacaaaaat tcgattgaat cctcgatagc cggtcatgac atcgcgaatt tttgttttat
    11641 ataagtaagt aattgtattt ttaaccagat tattcccaaa atcatggaaa ggacgcttat
    11701 tttcatcaaa ataggttcca tttgatagac gatcgccaat gaccatatct gcttcgccag
    11761 aacgtaattt ctcaagcaaa ccatgtgcag cttctgcagg ataagtatca tcaccatcta
    11821 ccatcaaata ataatcggca tcaatatcaa aaaacatttg acggattaca tttccttttc
    11881 cttgacgtgg ttctttcttt acgattgccc caccttgaac ggccaattcg tatgttcgat
    11941 cggtcgaatt attatcatag acataaatat ctgcttctgg taattccctt ttaaaatctt
    12001 caataacagt tgaaattgtc gcctcttcgt tataacaagg aatgagcacg gcaatttttt
    12061 cacgtgattc catttgtcac tctccttata taataaaact aatcttattt caagaatacc
    12121 atataat
//
LOCUS       DAXLPI010000076        11909 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXLPI010000076 DAXLPI010000000
VERSION     DAXLPI010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.076"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            119..361
                     /gene="yaaA"
                     /locus_tag="ACF3T5_001862"
     CDS             119..361
                     /gene="yaaA"
                     /locus_tag="ACF3T5_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFD3632463.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            348..1472
                     /gene="recF"
                     /locus_tag="ACF3T5_001863"
     CDS             348..1472
                     /gene="recF"
                     /locus_tag="ACF3T5_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFD3632464.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1469..3415
                     /gene="gyrB"
                     /locus_tag="ACF3T5_001864"
     CDS             1469..3415
                     /gene="gyrB"
                     /locus_tag="ACF3T5_001864"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFD3632465.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3435..5906
                     /gene="gyrA"
                     /locus_tag="ACF3T5_001865"
     CDS             3435..5906
                     /gene="gyrA"
                     /locus_tag="ACF3T5_001865"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFD3632466.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6142..6441
                     /gene="rpsF"
                     /locus_tag="ACF3T5_001866"
     CDS             6142..6441
                     /gene="rpsF"
                     /locus_tag="ACF3T5_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFD3632467.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6491..7003
                     /gene="ssb"
                     /locus_tag="ACF3T5_001867"
     CDS             6491..7003
                     /gene="ssb"
                     /locus_tag="ACF3T5_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFD3632468.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7029..7265
                     /gene="rpsR"
                     /locus_tag="ACF3T5_001868"
     CDS             7029..7265
                     /gene="rpsR"
                     /locus_tag="ACF3T5_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFD3632469.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7408..9381
                     /locus_tag="ACF3T5_001869"
     CDS             7408..9381
                     /locus_tag="ACF3T5_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFD3632470.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9387..9839
                     /gene="rplI"
                     /locus_tag="ACF3T5_001870"
     CDS             9387..9839
                     /gene="rplI"
                     /locus_tag="ACF3T5_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFD3632471.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10113..11480
                     /gene="dnaB"
                     /locus_tag="ACF3T5_001871"
     CDS             10113..11480
                     /gene="dnaB"
                     /locus_tag="ACF3T5_001871"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFD3632472.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT         3985 a         2160 c         2637 g         3127 t
ORIGIN      
        1 aatcaccttt tttagattaa atcgtcttat atggaaaaat atatggataa atttgtttta
       61 ttgcgaaaaa aaaggtataa tagagtatac ttttaataat gaaaatgagg gatgggcatt
      121 gaaacaacaa gtcattctaa atacggaatt catgacgttg ggccaaatgt tgaaagaagt
      181 cacagttatc ggtagtggcg gacaagcaaa atggtatttg gcagaaaaca ctgtgttagt
      241 cgatggcgaa ccagaaaatc gtcgtggacg caaactttat cccggcatga tggttgaagt
      301 tccagaagta ggaacttttt ttatggtaaa aaaaggaaac gacgataatg aggctgaata
      361 agctgtattt aaaaaattat cgaaattacg aagagctgaa catcgaattt tccaagaacc
      421 tcgtcatctt tttaggagaa aatgcgcaag gaaaaacgaa tcttttggaa agtatctacg
      481 tattagctat gacgcgaagt catcggacaa gtagtgagca ggaattgatt ggctggactg
      541 acgatcaggc gatgattcaa ggagagatca caaaaggttc atccactctt ccattagaaa
      601 tcttattatc taaaaaaggc cgaaaaacca agatcaacca tatcgaacag aaaaagttaa
      661 gtacttacgt gggacaaatg aatgtcattt tatttgcacc agaagatctt tctttagtaa
      721 aaggcagccc gcagatcagg cggaagtttt tagatatgga attaggccag atcaatccaa
      781 tctatcttta tgatcttgtc caataccaag caattttgaa acaaagaaac cagtacttga
      841 agcaattgaa tgaaaaaaaa caaactgatt tgctttattt ggatattttg tccgaacaac
      901 tagcagcgtt tggcagcaaa gtattgaaag caagagcaca gttcgtcaag cgattggagt
      961 tttgggctaa ttctttgcat cagcaaatca cccatcaaaa agagcagctg gaaatcgagt
     1021 atctcaccgc agtcgattct ttagaaacac atacgcaaga gcaaatccaa gaacaatttc
     1081 tcgcactttt gaaccaaaat aaaaagaaag acctttttcg gggaacaacg acagttggtc
     1141 ctcatcgtga cgatctttcc ttttttatta atcagaaaaa tgtgcagact tatggatcgc
     1201 aaggacagca aagaacaact gcactaagtg tcaaattagc agaaatcgat ttgattaaag
     1261 aagagacagg ggaatatcca atccttttgt tagatgatgt gatgagcgag ctggatgata
     1321 atcgtcaact ccatctgcta gaaacaatcg aaggaaaagt tcaaacgttc ttaacaacaa
     1381 cgacacttga ccatgtaaaa aataaaatga ctgttgaacc tgagattttt tatgttcaac
     1441 aagggaaact aaaaggagaa gaaactgaat gacagaagaa agaagtttag tagaacgcgc
     1501 taaagagtat gatgccagtc agattcaagt acttgaagga cttgaagccg ttcgtaaacg
     1561 acctggcatg tatatcggtt cgactagttc tgagggtctg catcaccttg tttgggaaat
     1621 tgtcgataat tcgatcgatg aagtattagc aggttttgca acgaaaatcc atgtgattat
     1681 tgaaaaagac aacagtatca ccgttattga cgacggtcgg gggatacctg tagatatcca
     1741 ggcaaaaact ggacgtcctg cagttgaaac ggtctttact gttttacatg ctggaggaaa
     1801 attcggcggt ggtgggtaca aagtttctgg tggacttcac ggagtaggat cttctgttgt
     1861 aaacgcgctt tctacacagc ttgatgtcaa agtatacaaa gaaggaaatg tgtactatca
     1921 agaatatcgt cgtggagctg tagtggacga cttaaagatt atcgaacaaa cggatcgcca
     1981 tggaacaacg gttcacttta cgccagaccc tgaaattttt acagagacaa ctgagtttga
     2041 tttttcaaaa ttggctaccc ggatccgtga acttgctttc ttgaaccgcg ggatgcgtat
     2101 atcgattgaa gacaaacgag aagaagaacc agtcatcaat gaataccatt atgacggcgg
     2161 gatcaaaagt tatgtcgaat acttgaatgc aaacaaaacc gtgatttttc cagaacctgt
     2221 atatttggaa ggtgaacagc aagatatcgc agttgaagtc tctatgcaat atacagacgg
     2281 ctaccattcc aatatcatga gttttgccaa caatatccat acgtatgaag gtggaacaca
     2341 tgaatcagga ttcaagactg ctttaactcg tgtgataaat gattatgccc gaaaacaaaa
     2401 gctgatgaaa gaaaatgacg acaacttgac cggtgaagac gtgcgagaag gattgacagc
     2461 tgttatttct atcaagcatc ctgaccctca attcgaagga caaacaaaaa caaaattggg
     2521 caattccgaa gtacggacag tcactgatcg gttatttagc gaacatttca tgaaattctt
     2581 gctggaaaac ccaagtgtgg gccggcaaat tgttgaaaaa ggattactag cttccagagc
     2641 tcgtctagca gcgaaacgtg cgcgtgaagt aacgagacgt aaaggtgctt tagagattag
     2701 taatctgcca ggaaaattag cggactgctc tagcaacgat cctgaaaaat gcgaattgtt
     2761 catcgtcgaa ggagattcgg ccggcggatc agctaagcaa ggtcgtaatc gagagtttca
     2821 agccatcttg cctattcgtg ggaaaatctt gaatgttgaa aaagcaagca tggacaagat
     2881 cttggcaaat gaagagatcc ggtctttatt tactgctatg ggtacagggt ttggagaaga
     2941 ttttgatgta tcgaaagctc gctaccacaa attagttatc atgactgatg ccgatgtcga
     3001 cggcgcgcat atccgtacat tgctgctgac tcttttttat cgttacatgc gtcctattgt
     3061 tgaagctgga tacgtctata ttgctcagcc acctttatac ggggtaaaac aagggaagaa
     3121 tattacgtat gtacaaccag gaaaaaatgc agaagaagag ttgaaacaag tcgttgcttc
     3181 acttcctgcc tcaccaaaac caagcgttca gcgttataaa ggacttggag aaatggatga
     3241 tcatcaacta tgggaaacaa ctatggaccc aagcaatcgg atgatggccc gcgtcagtgt
     3301 ggacgatgct attgcagcag atcagatatt tgaaatgttg atgggggatc gagtagaacc
     3361 ccgtcgtgca ttcatcgagg aaaatgcaca ctatgtgaag aacttagata tttaatcatg
     3421 aaggaggaaa aagaatgagt gaagaaatca gagaaaacat ccatgatgtc aatctaacca
     3481 gcgaaatgaa agactccttc atcgattatg cgatgagtgt tattgtcgca cgtgcacttc
     3541 ctgatgtcag agatggtctg aaacccgttc atcgccgtat tttatacggc atgaacgaat
     3601 taggtgtaac accggataaa gcccataaaa aatctgccag aatcgtcgga gatgtcatgg
     3661 gtaaatacca tcctcatgga gacattgcga tctatgagtc aatggtacgg atggcacagc
     3721 cattcagcta ccgttatatg ctagtggatg gtcatggaaa ctttggttct gttgacggtg
     3781 acggtgccgc cgctatgcgt tataccgaag cacggatgag caaaatcgct acagaaatgc
     3841 tgcgtgatat caataagaac actgttgatt tccaaagcaa ctatgatgat acagaaaaag
     3901 aaccagttgt actacctgct cgcttcccga atcttttagt gaacggaaca acggggatcg
     3961 ccgtaggtat ggccacgaat atcccacctc ataacttgtc tgaggtagta gcggctatcg
     4021 atttgctgat ggataatcct gatgtgacga ccaatgaatt gatggaagta ttacctggac
     4081 cagattttcc aacaggtggt ttggttatgg ggaaatcagg aatccgaaga gcgtatgaaa
     4141 caggtaaggg ttctattacc gtacgtgcaa aagttgagat tactgagatg ccaaatggta
     4201 aagaacggat catcgtgaca gaattgcctt atatggtcaa taaagcaaaa ttgatcgaac
     4261 ggatttctga actgcacaga gacaagcgta tcgaagggat cacagatttg cgggacgaat
     4321 cttcccgtga aggtatgcgg atcgtcattg acatccgtcg tgacgccagc gcatccgtta
     4381 tcttgaataa cttatataaa ttgacatcct tgcaaaattc atttggattc aatatgttgg
     4441 cgatcgaaaa aggtgtgccg aaagttttga gcttgaaaca aatccttgaa aactatatcg
     4501 aacatcaacg cgaagtcatc acacgacgga cggtctttga aaaagaaaaa gcagaagcaa
     4561 gagcacacat cttagaaggt ttgcgtattg ctttggatca tatcgatgaa atcattgcga
     4621 ttatccgcgg gtctaaatca gatgacgagg caaaagcaac aatgatcgaa cggttcgatt
     4681 tatccgatcg ccagtctcaa gctattttag atatgcgttt acgccgattg accggtttgg
     4741 aacgggaaaa aatcgaaaat gaataccaag aactcttaaa actgattgaa gatttaactg
     4801 acattctggc aagaccagaa cgtgttacag aaatcatcaa gaatgaatta gcagacttga
     4861 atcaacgatt tggtgataaa cgacgcacag aattactagt cggtgaggtc ttaagtctgg
     4921 aagatgaaga tttaatcgaa gaagaagaaa tcgttattac attgacaaac aatggttaca
     4981 tcaaacgttt ggcaaataat gaattcagag ctcaacgaag gggtggacgt ggtgtccaag
     5041 gaatgggtat ccacaatgac gacttcgtca aaaatctggt ttcttgttct acccatgata
     5101 cattgttatt ctttacgaat aacggaaaag tgtatcgggc aaaaggatac gaaattccag
     5161 aatacgggcg tacagccaaa ggaatcccaa tcatcaattt actaggtatc gattcgagtg
     5221 aaagcatcca ggcaatcatt gcggtgacgg gagaagcaga agaaggccac tatctcttct
     5281 ttacgacgcg tcaaggtaca gtaaaacgta cttctgtcac tgcttttgct aacatccgaa
     5341 gtaatggtct gatcgcaatt ggtttgaaag aagacgatga gctcgtcaat gtgctactga
     5401 cagatggtca atctaatatc attattggaa cacataacgg ctactctgtc actttcaaag
     5461 aagaagcagt ccgcgatatg ggacgtacag cttccggggt cagagggata cgcctgcgtg
     5521 aggaagatta tgtcgttggg gcagccttaa tgaaggaaaa tcaagaagta ttagtgatca
     5581 cagaaaaagg ttacggaaaa cggacaaaag tatcggaata tccagtgaaa ggccgcggag
     5641 gtaaagggat caaaacggcg aacatcactg aaaaaaatgg tccactagct ggattgacca
     5701 ctgtttctgg agatgaagac atcatgctga ttacagataa aggtgtcatt atccgtttca
     5761 acgtatcgac tgtttctcaa actggacgtt caacattagg cgtacgtttg atgaagatgg
     5821 aagctgatac gaaagttgtc acgatggcag cagtcgaacc agaagctgca gaacaaacag
     5881 ttgaagaaca aggatcagaa gaataaccat caagactaag tgtgtaagct agcaagaata
     5941 accgattaaa aaatctggtt attcctgctg gcttttttga ttagttgaat ttctcttaaa
     6001 atattcgaaa ctgttcattc tgtattgctt ttgaccaaaa tagagtgtat aattagagct
     6061 tgtgagtagt ctaattaatt agataactct ccttgctctt aggaacctaa gagccgaaag
     6121 tccacaggga ggtgaatatc aatggaaaac acgaaatatg aaatcatgta cattattcgt
     6181 ccaaacattg atgaagaagc aaaaactgct ttaatcgaac gtttcgatgc aatcttgaaa
     6241 gataacggag cagaagttat cgaatcaaaa gattgggaaa aacgccgttt cgcttatgaa
     6301 atgaacggtt accgtgaagg tatctatcac atcgttaaag tatcttctcc atcatcagca
     6361 aatgctgtca atgaatttga ccgtcttgct aaaatcaacg acgatatcat tcgtcacatg
     6421 attgttaaag aagaagctta attgttgttt catgtgaaac aatagaaaac gatgagagga
     6481 gatgactatt ttgattaata atgttgtatt agttggacgt ctgacgaaag atccagattt
     6541 gcgttacaca gcaagtggta cggcagtcgc aacatttact ttggctgtga atcgtaattt
     6601 tacaaaccaa agcggcaatc gcgaggctga ttttatcaac tgtgtgattt ggcgcaaatc
     6661 agcagaaact ttagcaaact atgctcgtaa aggaacactt ttaggtgtta ctggacgtat
     6721 ccaaactcgt tcttatgata atcagcaagg acaacgtgta tacgtgacag aagtagtagc
     6781 tgacaacttc caattgctgg aaagtcgttc tgcatctgaa aatcgccaac aaagcggcgg
     6841 attccaaagt tctggtcaat cagctggcgg atttggcgga aacaataact ctaatcaaac
     6901 atctcaatca tccaacggta tgcctgattt cgaccgtgat acttctgacc cattcggctc
     6961 aagttcaacg atcgacattt cagacgatga tttaccattc taaacgaatt tgataggagg
     7021 gaaatagaat ggcacaacaa agaagaggcg gacgtaaacg tcgtaaagta gactacattg
     7081 ctgctaacca tattgaatat atcgattata aagatatcga attactaaaa agatttattt
     7141 cagaacgcgg taaaatcttg ccacgtcgtg taactggtac aggcgctaaa aaccaacgta
     7201 aaattactgt tgcaatcaaa cgcgctcgta tcatgggctt gctacctttc gttagcgaag
     7261 actaataaat cctaggaatt gaatgactga tccttttgat cagctattca attccttttt
     7321 ttctgtaaaa ataacctcta ttcgtttagt cgctcgaatg acgaaagtta tggtaaaatc
     7381 aaattagtca tatatgggag gaaaattatg gaaaataaaa acaatcattt atccccgaaa
     7441 cctctcttcc ttatcctgtt tatattaatc gaagtattgg tagtcttaat tttaccattt
     7501 cgttttgtta gcgcttcggt cattctattg atgaatttat ggggtgttcg ctattggttg
     7561 aaaatgaaac gttggcttat tttaaataaa cgtgaaaaaa tccagcaggc ttcgactgta
     7621 gctgaaacca atctagctta tgtctcagaa gtcatgcctg taggagtgat tgcttatcag
     7681 ccatcaagcc aaaaaatcga atggctcaat ccattagcat tgaaaatcaa agatcaaagt
     7741 ggattaacag aggaagaatt gctagatact ttttttactg caaaagaaaa aaagaaaaac
     7801 caagtctatc tagcagataa aacatttcag tttgatatgg atacagtaaa gggtgttgtt
     7861 tattttaatg atattaccca aaaccagttg ttaaaactcc atcagacgaa cagtcagccg
     7921 gtgattggta ttttatctat tgataactat gcagatgcca ttgataaatt agatgacaaa
     7981 gagatttctt atttgaactc tttcgtcact acactagttt ctgactggat gaatgaatac
     8041 catgtctttt tcaaaagaat caacgcagaa cggtttttct tcgtagcaca gatgatcgat
     8101 ctgaaaaaga tgatggataa aaaatttgat ttgctggatc gctttcgagc agaagctgaa
     8161 aaacagagct cgcctatcac ccttagtatg gggattgctt atggctcaag aaccctcgat
     8221 gaaatcggtg gagaagctca gaataattta gatgttgctt tagtccgtgg aggcgaccaa
     8281 gttgtcgtga aagaggtaaa agaaggagca aaacctgttt attatggggg gaaatctgct
     8341 aagaatgtca aacgcacgcg tgttcgttct cgagcgatga gtaccgcttt gcgtcggatt
     8401 tttgatgaaa caggcgatgt gtttatcatg gggcacaagt ttccggatat ggatgcttta
     8461 ggctctgctt ttggtgtcag ttgtctggca cgcttcaacc agaaaaaagc ttacatcgtt
     8521 atcaatgaga acgagatcat tccagatgta gaacggtgtc ttgaagaaat ccataagcat
     8581 cctgaattag aaatgcaact aatctctcca gaacgtgcca tagagctgaa aaaagaagag
     8641 gacctcctcg tgatggtgga ttaccataaa ccttctctat cgatctctca gggcctatat
     8701 gagcaatttg ataagattgt gatcattgat caccatcgtc gtggggaaga gtttccaagt
     8761 aaacctctgc tgacttatat tgaatcttca gcttcttcag cttcagagct agtttctgag
     8821 ctgatccaat accaaagcag caaaacaaat cgcttagatc gttttgaggc cacattattg
     8881 cttgcaggga tggttgtaga tacaaatagt ttcaatgtcc gtacaactgc acgaactttt
     8941 gacgttgcca gctatttaag gtcttgtgga gcagacgcat ccttagtaca atatttattg
     9001 agctcggatt tagcgacata cttggagatc agcaatttga ttgctggaag cgactatgtg
     9061 acttctgata ttgttgtggc ggcagggaca gacgaaaaag agtataatag tgttatagca
     9121 gcaaaaacag cagatacatt gctttcgatg atcaatatca atgcagcttt cgtcattaca
     9181 aagcggacag acggcttgat tgggatcagt gccagaagta ccggcacgat caacgtccaa
     9241 atcatcatgg aatccttagg tggcggagga cacttcacaa acgcagctac tcaactggaa
     9301 aatacgacag ttgaggaaac aaaacagaag ctatttgctg caatcaaaga aaatatgaat
     9361 caaatatatg ataaggagta atccaaatga aagtaatttt tttacaagat gtaaaaggaa
     9421 aaggtaaaaa aggggacatc aaagaagttc cagcaggtta tgcacaaaat tatctaatca
     9481 aaaataactt ggcaaaagaa gcaacgaaaa gcagtatcag tcagttagca ggacaaaaga
     9541 aagcacaaga aaaacacgaa gctgaaatct tggaagaagc taaaaagtta aaagaattct
     9601 tggaaaaaga agatactgta gtcgaattga aggcgaaagc aggggaagac agccgtttgt
     9661 ttggttcgat cccttcaaaa caaattgctg aagggttaaa caaacaatac aaaatcaaat
     9721 tagataaacg aaaaatcgaa ttagaaaatc cgatccgttc actaggctac acaaacgtac
     9781 ctgttaagct tcatcatgag gtgacggcta cgatcaaagt tcatgtcgta caagaataac
     9841 aaatcttgat agcgatgttc agctggtaag tataaccaga aggaaaaaag acaaaccgaa
     9901 atttatctga gaaaacagct gcagcaatga aagcaaaagc tgcaaatcga tttcagattg
     9961 atttcggttt cttttttggg aacagcaaaa tagcataaag aaaacgagta taataaaaga
    10021 gaactatttt tttattacac tttcggtacg aatgatgact tttctctcat tttcttgtaa
    10081 aatgaagcaa ttgaagacaa ggagcgatac gtatgaatga agtgtggcag gatcgaatcc
    10141 caccgcaaaa tattgaagct gaacaagcgg ttttaggctc tgtttttctt gacgcagaaa
    10201 cgatcattga tgccatggag tacatagagc caaaagactt ttatcgtcgt ggccatcaaa
    10261 ttatttttga gacaatgatc gagttaaatg atcgtaatga agcgattgac gtggttactg
    10321 tcaaagaccg gctggaacaa gccaatttgt tagaagatgc cggtggttta agttatctct
    10381 cagacttggc attagcagtt ccgacagcag caaatatcgt ctactatgcg aaaatcgttg
    10441 aagaaaaatc attgctgcgt acattgatcc agacagcaac aggaatcgtt acgaaaggat
    10501 tcgagcaagg agaagaagtt caaacaattt tagatgatgc cgaacgcagt atcttggagg
    10561 tttcggaaaa aagaaatcga agcggctttt tatctattgc tgaagtactg aactccacga
    10621 ttgaaaatat cgatcgacta gctcaaaaca atgaagaaat cacaggtcta ccaactggct
    10681 atcaaggtct ggataaaatg acagcaggtt tccagcccga agaactaatc attcttgctg
    10741 cacgtccagc ggtaggtaaa acagcgtttg cattgaatat tgctcaaaat gtaggaacaa
    10801 aaacagatcg ttctgttgcg atatttagtt tggaaatggg ggcagaatct cttgtaaatc
    10861 ggatgctttg tgcagaaggt tcgattgagg ccagccattt acgtacaggt cagctttcgg
    10921 aagaagaatg gtcgaatctg atcgttgcga tggggagtct ttctcgagca aatattttta
    10981 tcgatgatac accagggatc aaaatctctg aaatccgtgc gaaatgtcgg aaactagcgc
    11041 aagaaaaagg caatctaggc ttgatattga tcgattattt gcagttgatt gaaggtaacg
    11101 gtaaagagaa ccgtcaacaa gaagtatcag atatctctcg ccaactgaaa aaactggcga
    11161 aagagctgaa agtacctgtc attgctcttt cacagttatc tcgtggagta gaacagcgac
    11221 aagacaaacg accagtgcta agtgatattc gtgaatccgg atcaattgaa caagatgcgg
    11281 acatcgtagc cttcttgtat cgagatgatt attatgaaag agaaggcgga gatgacgatg
    11341 atggaaacga gccaaaaaat gacaacgtca tcgaagtcat catcgagaag aaccgaagcg
    11401 gtgcacgagg aacagttgaa ctactgttta tcaaagaata caataagttt tcgtcgcttt
    11461 cgccaagaga agaattttaa agaagaggtt gtgagccttg agcaatcccg agagctctcg
    11521 taagaagatg caaggagtaa ccgcaggaac gactagagta cttaatttaa acagatctcc
    11581 aaagggattg tgacaaaagt cttgtcacag tccctttttc cgaataaacg gtgttcaatc
    11641 agctgaccct tggtattagc tcagcaaacg tcaaaatatg aggaactatt ttgacgtttg
    11701 tttctctaat actcaggtca aaacgctgat cttacgacct cttttcctta aacggtgttc
    11761 tcctgccaac tcctcggtca caagtcacaa tatcatcaaa catgagaagc agttttttga
    11821 aattgctcct tgtgactcag agttaaacgg caggctcaca acctctttct taaacggtgt
    11881 tcaaaagctt gcatctgcgg taatacgcc
//
LOCUS       DAXLPI010000077        11617 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXLPI010000077 DAXLPI010000000
VERSION     DAXLPI010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.077"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(234..719)
                     /locus_tag="ACF3T5_001872"
     CDS             complement(234..719)
                     /locus_tag="ACF3T5_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFD3632473.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    809..1154
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1199..1840
                     /locus_tag="ACF3T5_001873"
     CDS             1199..1840
                     /locus_tag="ACF3T5_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFD3632474.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1859..2494
                     /locus_tag="ACF3T5_001874"
     CDS             1859..2494
                     /locus_tag="ACF3T5_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFD3632475.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2512..3327
                     /locus_tag="ACF3T5_001875"
     CDS             2512..3327
                     /locus_tag="ACF3T5_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFD3632476.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3646..5082
                     /locus_tag="ACF3T5_001876"
     CDS             3646..5082
                     /locus_tag="ACF3T5_001876"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFD3632477.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5271..7718
                     /locus_tag="ACF3T5_001877"
     CDS             5271..7718
                     /locus_tag="ACF3T5_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFD3632478.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7948..9015
                     /gene="rlmN"
                     /locus_tag="ACF3T5_001878"
     CDS             7948..9015
                     /gene="rlmN"
                     /locus_tag="ACF3T5_001878"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFD3632479.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            9005..9211
                     /locus_tag="ACF3T5_001879"
     CDS             9005..9211
                     /locus_tag="ACF3T5_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632480.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(9208..10311)
                     /locus_tag="ACF3T5_001880"
     CDS             complement(9208..10311)
                     /locus_tag="ACF3T5_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFD3632481.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10864..11412
                     /gene="aac(6')"
                     /locus_tag="ACF3T5_001881"
     CDS             10864..11412
                     /gene="aac(6')"
                     /locus_tag="ACF3T5_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFD3632482.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3863 a         1956 c         2502 g         3296 t
ORIGIN      
        1 aaaagaataa aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt
       61 ttcgtagaat caataaatat ggatttggct aactatggaa gaatactcgt attagttttc
      121 agcgttcata ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt
      181 gctaaataaa gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa
      241 ttttcataaa ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc
      301 ataatcaacg tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata
      361 gcagcatcga tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc
      421 actgtacctg ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag
      481 cgcttttctt ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag
      541 atgacaagat agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga
      601 ccaggtaggg aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga
      661 aaagagatac ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc
      721 gcgcatcacc ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata
      781 atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag
      841 gtgtttttag agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct
      901 tggagtcgat tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt
      961 cgttgctaga tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga
     1021 accacgtgat taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg
     1081 gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat
     1141 aatacgtcct cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat
     1201 ggagtatttg attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt
     1261 atttttattt accttgattg gctcattacc attagggatt gttttagcat taggattgtc
     1321 tttgcatttt aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac
     1381 accgctgttg ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt
     1441 tgatcgtttc gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga
     1501 gatctttcgt ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt
     1561 gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt
     1621 tttaccttct gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat
     1681 attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt
     1741 actgcctttg ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcat
     1801 aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat
     1861 gattgaattg aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa
     1921 tctaatcatt ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac
     1981 cacgctttta cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga
     2041 cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt
     2101 ccaagacttc cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa
     2161 aatggtattg aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc
     2221 attagggatt agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca
     2281 gcgggtagcg attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc
     2341 aacaagtgct ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa
     2401 agaagatggg gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg
     2461 tgatcaatta ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa
     2521 accagttttc tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa
     2581 aaaacttcag cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta
     2641 gacgatacat ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat
     2701 attgatttag ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt
     2761 gactggtcaa tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc
     2821 tattccaaaa cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac
     2881 gatcaagtgc tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat
     2941 aagagattag gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa
     3001 gtattaaaag agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca
     3061 ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac
     3121 tattatttga aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag
     3181 agtgaggatt ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat
     3241 gaagccctag atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt
     3301 ggcgaagatg ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca
     3361 gtcccttttt ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaatg
     3421 gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttttcggt attaagccaa
     3481 atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact cgaaactaag
     3541 cgactttttc acaacctcct ttacataatt ttattttgtt gtggaagggg tttcgtgtta
     3601 ttctaaaaga ggaacgattg aaggttatag gaaaggaaga gatgaatgga acacaaagaa
     3661 cttacctcat ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa
     3721 ggagcttggc aagaagatgg gaaaggagaa tcagtctggg atcgatttgt acggatacct
     3781 ggaaagacat ttaaaggaac gaacggtgat ttggcagtag accactatca tcgttataaa
     3841 gaagatattg ctttaatgaa agaacaaggt ctgaaagctt accgcttctc tgtagcttgg
     3901 acacggattt ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc tttttatgag
     3961 agattgatcg acgaattgat tgaaaacgat attgaaccta tgttgacttt atatcattgg
     4021 gatcttccac aagcattgca agatgaatat aatggttggg aatcacgtca gatcattgaa
     4081 gatttcactc attatgcaga aacattattt gaggcattcc gcgggaaagt ccattactgg
     4141 attagtttga atgaacaaaa tatcttcact tcattaggtt atttgttagc agcacatccg
     4201 ccaggtgtga cagacccgaa acgaatgtat gaagttaacc atattgctaa tttagcaaat
     4261 gcatctgtca tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt
     4321 gcgtatacgc caaattatcc aattgacagt aatcctgaaa atgttctggc tgctgaaaat
     4381 gcagaagacc tgatggccaa ttattggatg gatgtctata tgtggggaaa atatccgatt
     4441 gctgccatga ggttcttaga agaaaaagga tgggcaccaa cgattgaagc tggcgatgca
     4501 gaattgctag aatctgctaa accagatttt cttgggatca attactatca aacggcgacg
     4561 aatgcattca atccattaaa cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa
     4621 ggttcatcag aagaaactgg agttcctgga atgtataaaa aagttgagaa tccatttgtc
     4681 gaacgaacaa attgggactg ggagattgac ccagaaggat taaggatcgg actgcgtcgt
     4741 atcacaagcc gttaccgcat gccggtactg attacggaaa atggcttagg agagtatgac
     4801 aagttgacag aagataaaca aatccatgac gactatcgaa tcaattactt acaaagccac
     4861 gtcaaagcca tcaaagaagc aatttcggat ggagcagaag tcctaggcta ttgcacctgg
     4921 tcttatacag atttgcttag ctggctgaac ggttatcaaa agcgatatgg atttgtatat
     4981 gttgatcaag acgaaacaca agaaggatct ttggaacgat ataagaaaga cagttattac
     5041 tggtaccaaa aactgattga agaaaatgga gagaaccttt agttcacata cttaaaacaa
     5101 gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa atcatttaga aattaagaaa
     5161 aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt
     5221 tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa atggcacaaa
     5281 agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc
     5341 aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct gtcttcggct
     5401 ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa
     5461 atacttacca aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga
     5521 atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg
     5581 gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata
     5641 tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata
     5701 acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac
     5761 agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta
     5821 atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat
     5881 tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga
     5941 aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaaaaag
     6001 aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat actgctgatg
     6061 aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca atcgacagtt
     6121 ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca ccaaaacaga
     6181 ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc tcattagaat
     6241 ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt gatttgcttg
     6301 attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa aatatcggcg
     6361 tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa gccagcttag
     6421 gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta agtaaaattg
     6481 tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg atggaagcac
     6541 cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc agtatggttt
     6601 cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta gaagtacctt
     6661 taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg cctcacttac
     6721 tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt atcacaagta
     6781 tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg aaaatggttg
     6841 aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg aaccctagaa
     6901 aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat gaaaaattcg
     6961 cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag aaaaacttgg
     7021 aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat gaattagctg
     7081 atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta gcgcaaatgg
     7141 ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg gatgttatca
     7201 caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca agcggtacag
     7261 attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga ggagatatgc
     7321 tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc atttctgacc
     7381 acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat tatgaagaga
     7441 aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca caagatgagc
     7501 tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt tcccttctcc
     7561 aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa ctggaagctc
     7621 atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt gagcaggaag
     7681 aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat gaagggttga
     7741 aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa atggtttttt
     7801 tccatctttt ctaatacggt atgatgtctt gtctttttag tttttgtgag attaaagttc
     7861 tattcgattt atttgtgaag tattgcatta aggaaatgaa tcatgtatag tatgtagcga
     7921 aactttgaga aaaagaggga aaatactttg gaaaaacagt ccatttatgg cttgacgaat
     7981 gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa aattccgagc tgcccaagtt
     8041 tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa tctatcgaaa
     8101 caattgatcg aaaaattatc ggagcatttt atcatcaatc ctctaaaaca gatggtcgtc
     8161 caagaagctt cggatggtac agtaaaatat ttgtttgaac tccctgataa aaacatgatt
     8221 gagactgtac tgatgagaca agaatatggc ttgtcagttt gtgtgacgac acaagtagga
     8281 tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg tgatctgact
     8341 gctggagaaa ttgttgcaca gatcatgatg gtccaacatt attttgatga gcgcaatctt
     8401 ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg agccatttga taattacgac
     8461 aatgtaatgg actttttgca tatcatcaat gatgccaaag gattggcaat tggtgctcgg
     8521 cacattacag tttccactag tggattagca cacaaaatca aggaattcgc taataacggc
     8581 ttgcaagtca atctggctat ttccttgcac gcgccgaata atgaggtgcg aacatcgatc
     8641 atgcggatca accgaagttt cccaattgag aaattgatgg aagctgtgga tgagtattta
     8701 gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt aaatgataga
     8761 ccagagcatg cgcaacaatt agcagatcta ttaaaagata agaaaaaatt agcatatgtc
     8821 aacttgattc catataatcc agtcagtgaa catgatcaat attctcgcag tccaaaagct
     8881 gatgtattga gattttatga tgtattgaag aaaaacggcg taaactgtgt gatacgtaaa
     8941 gaacatggta cagatattga tgcagcttgc ggacaattgc gaagcaagca aatgaaaaaa
     9001 acagttgcca aataatcgta tcctactcgt tttttcaatt accataaaag gagcagtttt
     9061 cttaattaag gaactgttca acttaaataa gagaaaagat tctatagcga aagctataaa
     9121 gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt tgatgacagc
     9181 ctgcttgatg ggggttttag caattattta actgattttc ttatatcctt gctggctttc
     9241 ttgtgtagca tgataagttt ctacgtcaag aatggcctgt ttaaattttt ctcgggtgat
     9301 ttcataaggc acatgtttaa tatcatttgt tgccatggct ttatctaaga ttgcttctaa
     9361 atcggtcgtg acattgagat tcatgtcggt taacgatact ggaagttggt tctttttcat
     9421 aaacacatag attttatcaa acaaatcgaa ctgctgatcc atcatcaata aggtcagtac
     9481 accataactg acagatgctc cgtgaaggta tccctccatt ttttctagaa cagtcgtacc
     9541 gtaatagaat gagtgtgcca tagcagaatt atagttattg ttaactaaaa cagaaaccaa
     9601 tccggtagta acaatgatat ccgaaacgac ttgttcaatt gcatatgagg gacgatcgtc
     9661 ggcactatct ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc acatttttcc
     9721 gatttggatt cctaatctat ccgtgtagcc tagatcatcg ccacgagcag aaaaagtaga
     9781 ttcgacttct ttggataaag catcgccaat acctgcccat aagtatttat caggtgcttc
     9841 ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa agaagagtcc
     9901 gtccattacg ccttcttcgt tatagatgac acataccgca gtgacgggcg aacagttcga
     9961 accaatagtg ggaaaggtga agactggttt agctagtttt tcaccaatga cttttacagt
    10021 atcgcaagca cggccaccac cgactgcaaa caggacgtct gctttttgga cactttcatg
    10081 ctggatcaac tgttcaatat ttccataagt tgaattaccc ccgtaccaga gactggcgac
    10141 aacctctatt tcctgtgtca aagctacttt tattttgttt tctgcctttg aaagtgcagt
    10201 cttccctcca atcagcacta cttttgtccc atattctttg acgattccat ttgctttttc
    10261 atacgcatct gttccaatgg tataatttgg taagaaaaca ctttgactca tattcatttc
    10321 cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga ctgcggctgt
    10381 agatggagta gaggtgaacc taagacagga taagtgatga ataaggtggc atgaacacct
    10441 tgaaatcatc aataagtatc ctaaaaacga acaagtacaa aaacattgaa aacgacaata
    10501 atgaattttt gttcctctta gtatttctac cgattccctt tgcttttttg taattatcta
    10561 aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa ttcataattg
    10621 gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt ttttcctcca
    10681 attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata atttttttat
    10741 tttataagta agataaataa tgaaagaata gagatactga ttggtagcta aagatttttg
    10801 agcgagaaca tttcgttttt tcaaaaggac tgttagaata agcaagaaca agggggaaga
    10861 cgtatgataa tcagtgaatt tgaccgtaat aatccagtat tgaaagatca gctttctgat
    10921 ttactgagac tgacttggcc ggaagaatat ggagacagct cggcagaaga agtagaagaa
    10981 atgatgaatc cagaacgaat cgcggtagca gcggtagacc aagatgagtt agtaggattt
    11041 attggtgcaa tccctcaata cggtatcaca ggttgggaat tgcatccatt agttgtagaa
    11101 agctcccgac gaaagaacca aataggtact cgattagtca attacttaga aaaagaagta
    11161 gcttccagag gaggaatcac gatttattta ggtacggatg atttagacca tggaacaacg
    11221 ttaagtcaaa cggacctgta tgagcataca tttgataaag tggcttccat ccagaacctt
    11281 cgtgaacatc cgtatgaatt ctatgaaaaa ttaggttata aaatcgtagg tgtcttacca
    11341 aatgcaaatg gctgggacaa accggatatt tggatggcaa aaacgattat tcctcgacca
    11401 aattctcaat aataaaaaga ggttgtgaca gaagtaatca gcttcaagaa ataagaagga
    11461 atttacgaaa attacttctt gaatttttgt gaaattccaa cttatttccg aagaagctac
    11521 ttttggaaca ccgtgtacca gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa
    11581 aattttgtga aaatggcttt ctacattttt gcataaa
//
LOCUS       DAXLPI010000078        11542 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXLPI010000078 DAXLPI010000000
VERSION     DAXLPI010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.078"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            398..790
                     /gene="merR"
                     /locus_tag="ACF3T5_001882"
     CDS             398..790
                     /gene="merR"
                     /locus_tag="ACF3T5_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFD3632483.1"
                     /translation="MIYRISEFADKCGVNKETIRYYERKNLLQEPHRTEAGYRIYSYD
                     DVKRVGFIKRIQELSFSLSEIYKLLGVVDKDEVRCQDMFEFVSKKQKEVQKQIEDLKR
                     IETMLDDLKQRCPDEKQLHSCPIIETLT"
     gene            815..2455
                     /gene="merA"
                     /locus_tag="ACF3T5_001883"
     CDS             815..2455
                     /gene="merA"
                     /locus_tag="ACF3T5_001883"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFD3632484.1"
                     /translation="MNKFKVNIQGMTCTGCEKHVESALEKIGAKNIESNFRRGEAVFE
                     LPDDIEVESAIKAIDEANYQAGEIEEVSSLENVALINEDDYDLLIIGSGAAAFSSAIK
                     AIEYGAKVGMIERGTVGGTCVNIGCVPSKTLLRAGEINHLSKDNPFKGLQTSAGEVDL
                     ASLITQKDKLVSELRNQKYMDLIDEYNFDLIKGEAKFVDASTVEVNGAKLSAKRFLIA
                     TGASPSLPQISGLEKMDYLTSTTLLELKKIPKRLTVIGSGYIGMELGQLFHHLGSEIT
                     LMQRSERLLKEYDPEISESVEKALIEQGINLVKGATFERVEQSGEIKKVYITVNGSKE
                     VIESDQLLVATGRKPNTDSLNLSAAGVETGKNNEILINDFGQTSNEKIYAAGDVTLGP
                     QFVYVAAYEGGIITDNAIGGLNKKIDLSVVPAVTFTNPTVATVGLTEEQAKEKGYDVK
                     TSVLPLDAVPRAIVNRETTGVFKLVADAETLKVLGVHIVSENAGDVIYAASLAVKFGL
                     TIEDLTETLAPYLTMAEGLKLAALTFDKDVSKLSCCAG"
     gene            2697..4019
                     /locus_tag="ACF3T5_001884"
     CDS             2697..4019
                     /locus_tag="ACF3T5_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFD3632485.1"
                     /translation="MSDLLSLPDIKTIEPPQENETDMMFKVEAVGPPERCPECGFDKL
                     YKHSSRNQLIMDLPIRLKRVGLQLNRRRYKCRECGSTFWERLISVDEKRSMTKRLLKS
                     IQEQSMSKTFVEVAESVGVDEKTIRNVFKDYVALKEREYQFETPKWLGIDEIHIIRRP
                     RLVLTNIERRTIYDIKPNRNKETVIQRLSEISDRTYIEYVTMDMWKPYKDAVNTILPH
                     AKVVVDKFHVVRMANQALDNVRKSLKAHMSQKERRTLMRERFILLKRKHDLNERESFL
                     LDTWLGNLPALKEAYELKEEFYWIWDTPDPDEGRLCYSQWRHRCMSSNSKDAYKDLVR
                     AVDNWHVEIFNYFDKRLTNAYTESINSIIRQVERMGRGYSFDALRAKILFNEKLHKKR
                     KPRFNSSAFNKAMLYDTFNWYEVNDHDITDNFGVDFSTLIKNLEKGDL"
     gene            4044..4307
                     /locus_tag="ACF3T5_001885"
                     /pseudo
     CDS             4044..4307
                     /locus_tag="ACF3T5_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043131.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(4354..4584)
                     /locus_tag="ACF3T5_001886"
     CDS             complement(4354..4584)
                     /locus_tag="ACF3T5_001886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632486.1"
                     /translation="MNKVHVYNHNKFVKEINSDTNSIAKEIASQSPFAELKMTDEYDN
                     LILTTKGFFLDDVPNQIWLKNELMPALTNLQI"
     gene            complement(4854..5339)
                     /gene="vanZ-A"
                     /locus_tag="ACF3T5_001887"
     CDS             complement(4854..5339)
                     /gene="vanZ-A"
                     /locus_tag="ACF3T5_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFD3632487.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(5492..6382)
                     /locus_tag="ACF3T5_001888"
     CDS             complement(5492..6382)
                     /locus_tag="ACF3T5_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFD3632488.1"
                     /translation="MLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLENSGTSE
                     NTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMS
                     KEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGL
                     SLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHS
                     AIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGN
                     NIDGVIVTVFPGSTHTNSRR"
     gene            complement(6832..7440)
                     /locus_tag="ACF3T5_001889"
     CDS             complement(6832..7440)
                     /locus_tag="ACF3T5_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFD3632489.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(7446..8477)
                     /gene="vanA"
                     /locus_tag="ACF3T5_001890"
     CDS             complement(7446..8477)
                     /gene="vanA"
                     /locus_tag="ACF3T5_001890"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFD3632490.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(8470..9438)
                     /locus_tag="ACF3T5_001891"
     CDS             complement(8470..9438)
                     /locus_tag="ACF3T5_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFD3632491.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            complement(9653..10807)
                     /locus_tag="ACF3T5_001892"
     CDS             complement(9653..10807)
                     /locus_tag="ACF3T5_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFD3632492.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(10785..11480)
                     /gene="vanR-A"
                     /locus_tag="ACF3T5_001893"
     CDS             complement(10785..11480)
                     /gene="vanR-A"
                     /locus_tag="ACF3T5_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFD3632493.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
BASE COUNT         3541 a         2252 c         2144 g         3605 t
ORIGIN      
        1 atatcacctg atactttttt ttctataaaa tcaactgtta aagttatttc tcttgttgac
       61 attggcgaac gtgcttctat ttgatataaa tcaaaatcgt atttttgatg attatttatt
      121 cctctcattt ctgttactgt cgcttttata acttttaaaa tattaccttc aaaatttatt
      181 ttgggtcttc ggaatatata gtggaattac tttcttaata gtttaaaaac aactggtgtt
      241 taaatcggat tttctggtgg gatgttagga caatcaatag tattatataa aagtatcaat
      301 cacatacgag tgatacgttg gggaaaaata aaatagtcca atttacttga ccgcgtacta
      361 tggtacacgg tttataatat tcatggggtg aaataagatg atttatcgca ttagtgagtt
      421 tgcagataaa tgtggagtta ataaagaaac gattagatat tacgagagaa aaaatttatt
      481 acaagaacct caccgaacgg aagctggtta tcggatatat tcatatgatg acgttaagcg
      541 tgttgggttt attaaacgaa tacaggaact tagtttttct ttaagcgaga tttataaatt
      601 acttggtgtt gtagataaag atgaagttcg ttgtcaagac atgttcgaat ttgtttctaa
      661 aaaacaaaag gaagtgcaaa aacaaataga ggatttaaaa cgaattgaaa ctatgttaga
      721 cgacttaaaa caacgatgtc cagatgaaaa gcaattacat tcgtgtccaa taatagaaac
      781 attaacatga gacattaacg aaaggggctt ttttatgaat aaatttaagg taaacattca
      841 aggaatgact tgtacgggtt gtgaaaaaca cgtagaatca gcacttgaaa agataggtgc
      901 taaaaatatt gagtctaatt ttcgtcgtgg tgaagcagta tttgaactgc ccgatgatat
      961 tgaggttgaa agtgcaataa aggcgattga tgaagcaaat taccaagccg gagaaattga
     1021 agaagtatca tcactagaaa acgtggcgtt aattaatgaa gatgattatg accttcttat
     1081 tattggttct ggtgctgctg ccttttcttc ggcaattaaa gctatagaat acggtgcaaa
     1141 agttggaatg attgagcgtg gaacggttgg gggaacctgt gtgaatattg gctgtgttcc
     1201 gtcaaaaact cttcttaggg caggggaaat caatcattta tcaaaagaca atccgtttaa
     1261 aggtttacaa acatccgctg gagaagtgga tttagctagt ttaatcacgc aaaaggataa
     1321 attggtgagc gaacttcgga atcaaaaata tatggattta attgatgaat ataattttga
     1381 tttaattaaa ggtgaagcaa aattcgttga tgctagtacg gttgaggtca atggggcaaa
     1441 gttatctgca aaacgctttt taattgcaac aggtgcatcg ccttcgttgc cccaaatttc
     1501 aggacttgaa aaaatggact atttaactag tacaacactt cttgagttaa agaaaatacc
     1561 aaaacgatta actgtaattg gctcaggata cattggaatg gagcttggac aactatttca
     1621 tcatttaggt tcagaaataa cgcttatgca aagaagtgag cgacttttaa aggagtatga
     1681 tcctgagatt tcagagtcag ttgaaaaagc gttaattgaa cagggtataa accttgtcaa
     1741 aggggcaact tttgagcgtg ttgaacaaag tggagagata aaaaaggttt acataacagt
     1801 aaatggcagt aaagaagtca ttgaatcaga tcagttactt gttgccactg gaagaaaacc
     1861 aaatacggat tctttaaatt taagtgcagc aggtgttgaa actggaaaaa ataatgaaat
     1921 cctgatcaat gattttggtc aaacaagtaa tgaaaagatt tatgcagcag gagatgtgac
     1981 gttaggaccg caatttgtat atgtagcagc ctatgaaggt ggaattatta ctgataatgc
     2041 tatcggtgga ttaaacaaaa aaatagattt atcggtagtt cctgctgtta cgtttacgaa
     2101 tccgacggtt gcaacggttg gtttaacaga agaacaagca aaagagaaag ggtatgatgt
     2161 gaagacatct gtattacctt tagatgctgt tccaagagca attgtaaacc gtgaaacaac
     2221 cggtgtattt aaactagtag cagatgcaga aacactaaaa gtattagggg ttcatattgt
     2281 atctgagaat gcaggagatg tcatctatgc agcatcatta gctgttaaat ttggcttaac
     2341 gatagaagat ttaacagaaa ccctagcacc atatttaaca atggctgaag ggctaaaatt
     2401 agctgcactt acgttcgata aagatgtttc gaaattatct tgttgtgcag gctaagggaa
     2461 ctttttttac aactccctat tcaagtgaac cagctaatac tgtactaaag gtgtgcagta
     2521 gtttagcaaa gtctacaaaa gatttcatac actattttgc gatcacccat tatataaatg
     2581 ctttaaggga ttttctaagt gcatttgtgg tcacaaaaat aatttaacaa tgatgatttc
     2641 gacatcaaat ctataattga taccttaaca ccgcagagta ataaaggatg aacaatatgt
     2701 cagacttatt atccctacca gacattaaaa caatagaacc accacaagaa aatgaaaccg
     2761 atatgatgtt taaagttgaa gcagtcggac cacctgaacg ttgtcctgaa tgtggttttg
     2821 acaagttgta caaacacagt tcaagaaatc aactaattat ggatttgccc attcgtttaa
     2881 agcgagtggg cttacaattg aaccgtagac gatacaagtg tcgtgaatgc ggatctacct
     2941 tctgggaacg cctaatatct gtagatgaaa agcgtagtat gaccaaaagg cttttaaagt
     3001 ccattcaaga gcaatccatg tctaagacct ttgtagaagt cgcagaaagc gttggtgttg
     3061 acgagaaaac tattaggaac gtttttaagg actatgtggc actcaaagaa cgtgaatacc
     3121 agtttgaaac tcctaagtgg cttgggatag acgagataca tattatccgt agacctcggc
     3181 ttgtattgac taatattgaa cgcaggacta tttatgacat caagccaaac cgtaacaagg
     3241 aaacagtcat ccaacgtctt tcagaaatca gtgacaggac ttatattgag tacgtcacaa
     3301 tggatatgtg gaagccttac aaagacgcag tgaacactat ccttccacac gctaaagttg
     3361 tcgtagataa gtttcatgta gttagaatgg ctaatcaagc cttagataac gtcagaaagt
     3421 ctttgaaagc ccatatgagc caaaaagaaa gacgtaccct tatgcgtgaa aggtttatcc
     3481 ttctaaagcg taaacacgat ctaaatgaac gtgaatcatt cctcttagat acttggttag
     3541 gtaatcttcc tgctttaaaa gaagcctatg aactcaaaga agagttttac tggatatggg
     3601 atactcctga tccagatgaa ggtcgtcttt gttatagtca atggagacac cgttgtatgt
     3661 ccagtaactc taaagacgca tataaagacc tcgtgagagc cgtagacaac tggcatgtcg
     3721 aaatattcaa ctactttgat aaaaggctca ctaacgctta tacggagtca attaacagta
     3781 ttattaggca ggtagagcga atgggtagag gttactcgtt tgatgcctta cgagccaaaa
     3841 tccttttcaa tgagaagctc cataaaaagc gtaagccacg atttaattca agtgctttca
     3901 ataaagctat gttatacgat actttcaatt ggtatgaagt gaatgatcac gacattacag
     3961 acaactttgg tgtcgatttt tccacactta ttaagaattt ggagaagggt gatttataag
     4021 cccttttcca ccatgaaatc cgaatacccg aaatatataa atatcaagca gaatctgaac
     4081 gaaagaatat aaaagaaaga caagcccagg gaataaagat tgctaaagac ggtaaaaaat
     4141 atagaggtag aaaaccttta tttacaaagg atagccctag attacagcat gcttttgaat
     4201 tatataagaa tggatattca gacaaagagg ttcaaaattt aactggtatt aatcatgaaa
     4261 catttagaag atataggatt aaacataata ttcaacgcaa tatataaaat aacctcacct
     4321 aattatacta atgtgatcag gtgaggttat tttttaaatc tgtaaatttg taagagctgg
     4381 cattaactca ttttttagcc atatctgatt aggtacatca tctaggaaaa agccttttgt
     4441 tgttaaaatt aagttatcgt attcatctgt catttttaat tcagcaaatg gggattgact
     4501 agcaatttct ttagcaatgc tattcgtgtc gctattaatt tcttttacaa atttattatg
     4561 attatataca tgtaccttat tcattttatt tattctcctt tatggggtac cgtcaggaaa
     4621 atgcggattt acaacgttaa ggaaatttat taaagagacc aattcctcag cactaaaatt
     4681 gataattggg ctttctttgt tactatagtg ttaggtaagc tttttacttt atcaccggtt
     4741 ttcccctcac ttcacaccta cgggcgagtt tccccacatc ccgttttcca tctaatccaa
     4801 ttcattcaat atctttatgt gtatgaaaga tcagattgct tgatttagac atcttacacg
     4861 taatttattc ttaaatgggt acggtaaacg agcaataata cgagcaaaag tatacctaca
     4921 aaaataataa ctctgtctaa ttttttttga ttcatatgct tattgcttaa accatataat
     4981 ttcagtccaa gaaagcctcc aacagtattt gtaattacat ctgttatgtc tgtcgctcca
     5041 atagcgaaga taaattgaat tatttcaaaa gtaagactta aaaccagtac aaaagcaaac
     5101 ttaggtaaaa atccgatttc tttaaaattg acattcaaaa gcaagccaaa tggaataaag
     5161 attataacat tatctatcat ctctctgaaa ttcccagtag cagtaaatgg agtcaagtta
     5221 agacttcttt gatgataatt aaatactgat aaaatattgt attgtaattt gaataacact
     5281 aaccagatta gtgtcactaa atataaagct agcaatcctc tagataatat ttttcccaat
     5341 ataatttcct ctaattcttt tgtttaagtt gtatcattag tatagcaatg tcctagttaa
     5401 cgtgggtttt aaattaaaaa gctaacgtta ccccaaaagt gctagtacaa taatgctgtt
     5461 cattaatttc gttggtttca ttccgccatc cttacctcct tgaattagta tgtgttgatc
     5521 cgggaaacac tgtcacaatt acaccgtcta tattgtttcc tgatatctca taacgaagat
     5581 tagtcggcac atgaatggtg gtatttttag taacaggata ataaaagatc tcatattttt
     5641 ccccatttac actaacagaa atggtttttt cttcttttag gtaatccata tattcctcga
     5701 gaacgaaatt cttttctttc ataatcgcac tatgtggtaa accaacatag cgaatatgcc
     5761 atggttcata ttgaattcct gttaactctg ttttgtcctc tggataacgt aaaatgaacc
     5821 cgtatttcca agcattttct tctatccact ttccttcagg ggctcgttcc attttcgtca
     5881 agcttgatcc tacatctagt gataaacctg aattatgctc actataacct gctggtaagg
     5941 catactcagc ccccatttct tggtaaagca cactttgctc atcaaagtct cgatagccac
     6001 tattaataat aaaatgacta acgccaccct ttacagcatc attgaccatc tctgaaaatt
     6061 tttgtgctat ttcttttgac atataaatat tactatcaag caacccgtat ccatttatta
     6121 attcgtcatg tttagataaa ttcacgatat ctgacttcac actttcttgg cgaacaggat
     6181 atttactatt gattaatagc agatttcctt gataaacctg ttcttctgta attgttttct
     6241 cttgggtatt ttcagaagtc ccactatttt ctaaatgttc tttgggattt tgatcataat
     6301 tttgaaattc gactttttcc tgagaaaaca gtgcttcatt aacgtagtca taacctaagt
     6361 atattaagaa taacaataac aataaaaaaa aacaacttct tcattttcag tctcctcctt
     6421 aactatatag ttggaagtat gaaatatttc tagaatcttc cccttaaacg gcacaatctc
     6481 gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa ttatttcgcc ctactggatt
     6541 tactattatc accaatgtag atgaattttc ataagtgata cccatgctga atttaacgag
     6601 gctgaactgc ctgttgacaa tgggcggtgt gaaataccac ccctctatgc gatcagtctt
     6661 attatcaggc tatcttgccg cagcgcacgc agataatgac cgcaatatcg ctctgcctta
     6721 catcttcaat actatctact atatataaat tccctaagaa ccagttacat acgtcgggtt
     6781 tcctgccgaa agagttagct tatatagttt gtccgtgcaa cggttaaaag tttatttaac
     6841 ggggaaatca aaatagctat tggggtatgg ttcgtctctt aatacatagt gccaccattc
     6901 gaggctatat gcttcaaacc cactgttttc catgatggag cgcaaacgtc tgcgattttg
     6961 cgcttcattg catgatattc catttgccgc atgatgagag cgttcatcca taaaatcaaa
     7021 tcggctcccc attggtacaa gctcacccgt gtctaatcga taaagcgtaa gatcaatggc
     7081 actgccgcgg ctatggcttg attttgaagc cacgtatcct tttgaaatca tctcagttcg
     7141 gtcaatattg ggataataac tttcctttgt caggttattt tccggctgtg cagcccattg
     7201 cataaaacag tttacagcac gcttaggacg gtaaccgtcc catagaagca atccgtaccc
     7261 ttgggtagca gccagttctt ttgccttcaa aagcgattca gccaactcgt atgtccctac
     7321 aatgcgattt acttcataac cgtcaaccgg ttttccggtg aaattatccc aagtggcata
     7381 tttagcgtcc caacgaacac cgtgtactat ttcatctaaa aaagtaaatc ctatttccat
     7441 gcttatcacc cctttaacgc taatacgatc aagcggtcaa tcagttcggg aagtgcaata
     7501 cctgcagcgg ccatcatacg gggataacga ctgtatgacg tgaaaccggg cagagtattg
     7561 acttcgttca gtacaatgcg gccgttatct tgtaaaaaca tatccacacg ggctagacct
     7621 ctacagccga gcgctttata tatttttttt gccgtttcct gtatccgtcc tcgctcctct
     7681 gctgaaaggt ctgcgggaac ggttataact gcgttttcag agcctttttc cggctcgact
     7741 tcctgatgaa tacgaaagat tccgtactgc agcctgattt ggtccacctc gccaacaact
     7801 aacgcggcac tgtttcccaa taccgcacaa ccgacctcac agcccgaaac agcctgctca
     7861 attaagattt tgctgtcata ttgtcttgcc gattcaattg cgtagtccaa ttcgtccgcg
     7921 ctattgactt ttttcacacc gaaggatgag cctgaacgcg ccggcttaac aaaaacagga
     7981 taggtaaacg tagctgccac cggcctatca tctttattaa taacccaaaa ggcgggagta
     8041 gctatcccag catttttcgc aacgatgtat gtcaacgatt tgtccataca aattgctgag
     8101 ctttgaatat cgcagcctac aaaagggata ccggacaatt caaacagacc ttgtatggat
     8161 ccatcttcac ctgacttgcc atgcaaagct gaaaatgcta catcaacatg gttgatttca
     8221 tattcatggt tctttttaac aagtaatccg tgcatttttt tatccggcga gagtacagct
     8281 gaatagcaat tgtcgttttc ccattccgcg caaggttttt cgcacatttt ccatacacca
     8341 gatttcgtaa ttccaatgta taacggctcg tatttttctt tattaatgtt agcggctatc
     8401 tctattgcag attttaccga tacgtcatgc tcctctgagc aacccccaaa cagtattgca
     8461 acttttattc tattcatgct cctgtctcct ttcaaaatcc aaacagtttt taatggtttt
     8521 ttcaacggta tcacgcaacg cttgctcggt ataataggcc gtatgcggtg tgattatcac
     8581 gttaggcatt ctttgaagtt taagtaaaaa ttgattatca attggttttt gggtgcaatc
     8641 agagtagaaa aactcttcct ctccttccaa tacatccaat gcggcaccgc ccagtttccc
     8701 gttttctaat gctttaacca actcataggt atctacaagt ggaccgcgcc cagtattgat
     8761 aagaaatgct ccttgcttca ttctctgtat ttgttcgtgg ctgataatat agtgcgtatc
     8821 cgtattgagc ggcacatgaa gcgtaacgat atcgctattt tgcagcaact catcaaacgg
     8881 tacatagttt acctctatac ttcggctgcg actataagcc aacactttac atccaaatcc
     8941 tcgcagccgc tcaataaccg ctttgcctat ctggcccgtt cccaccacac caactgtcat
     9001 gtcgctgagt accttgccac ggtcgctgtc caacctgaaa tcatgttttt ccacagagcg
     9061 cacaatcgat tttacgttgc gtactgccat aagaattagc atcatagtat aatcggcaac
     9121 gctatccggc gagtacgcca cattgtcgac agtgatgccc attctcttag cagcagttgt
     9181 atctatatga ttgcagccga tgcttcgggt agaaatatat ttcacaccgg ctctcttcag
     9241 cgcaagaaga atagaggcgg aaatctctga tttatgtccc acactgatac attgattgaa
     9301 aggcgcggat ttggcgttgg attccgacac gttggcgtta attatcgttg ccataacgcc
     9361 aaagcgaggc gaaagagcat ggaatgcatc tgcctcatcc tgctcacatc cataaacagt
     9421 aatgccgatg ttattcataa ttaagaccaa ccctttctgt gaaaggcacc aaataaaacg
     9481 gggatgccaa tggtaatctt gtcaaatctt tctctgaaga acgaaaacgg ctcgttctta
     9541 cggtattaag tctaccgtgt aagaacgagc cgtttcttaa tattaaattg ttaatttcct
     9601 aagaaaaagt aaagataacc ttgagatttt cctaaaaaat tatatacatc tcttaggacc
     9661 tccttttatc aaccaagtct ggcatcgctg gaagctctac cctaaacgtc gtatagttat
     9721 cattgctttc cgcgtaaatc tgccctccat gctgaacaat aatttctttt gcaatcgcca
     9781 atccaagtcc cgcgccaccc gtatcggaag aacgagcatt gtccagccta tagaactttt
     9841 caaatatggc agctagctta tcttttggga tgcttccagt gttcttgaat tcgattgaca
     9901 ccacatcccc ggagaggccc gcggtaatgt caatgatgct gttatcctca ctgtatgcag
     9961 cggcgttttt caaaatgttg ttaaagactc tcgcgagttt atcagggtcg ccggacacgg
    10021 tcagatcctc gggggcgtga ataaccgcct gttttccatg tgcggaaagc tgaggataaa
    10081 attcatcggt catctgcacc agcatatagt ataggtctat gtgcgttttt gttagcgtta
    10141 tcgtttgtag gttataccgt gtaatctcaa aaaactcgtc gattagctgt tcgagtcgat
    10201 acgctttgtc caacgtgata tgcacatact ttgccttttg atctaccggc atgtctggag
    10261 cctcgtcaag caggctcaaa taaccgataa tggatgtaag gggcgtttta atatcgtgcg
    10321 ccaagtacat aacaacgtca ttttttcttt gttcggccag ctttgcatcc tgctctcgct
    10381 tttccagagt ccgttttaat gtgttgagct tttgttccat aacatccatt tccgcagaaa
    10441 gctcaatttg tttatcttcg ttctgaataa gtacatcaat gccggtattt atctcgtcaa
    10501 agtattttgc gaattttgaa agcatgacgc gacatagaat aagaatacta atgacaatcg
    10561 ccacataaat aaagatatct atattgttcc gtatggaata ttgatataat ttcatcgcgt
    10621 ccaggtgatt taagtcatat ttgttttcca aaatacttaa gatccaatcc ccaagtttcc
    10681 ctcggatcat tgaacgaata tacaacacga atacaattgc taccacaaca attgcaacga
    10741 tatacatgta aagttttcgt tctagtttgg aatagtcgtt ttttttattt ttcaatttta
    10801 taaccaaccc cccataccgt ttttatatat ttcggattat caatggtgtc gttcattttt
    10861 tcgcgcaaat gccggatatg cacggtgatg gtgttgttgc tcttgctgaa atattcgtcg
    10921 ccccatatct catgaaatag cagctcggag ctaaccacat tccccttgtt ttcacagagg
    10981 attcgcagta ttgaaaactc ggtgggagta agggataact gcttctcgtt cagataacac
    11041 tcatgggtgt taacattaat gacaaggccg gagtggacga taacattttc gttctgctcc
    11101 tttactccac tgaatttttt gtatcggcgc aactgggcct ttacccgagc aattaactcc
    11161 agtgggcgaa agggcttcgt tatataatca tccgcgccga ttgttaaccc tgtaatttta
    11221 tctacctctg tatctttccc ggtcagcatg ataatcggat aggtgtgctt gtcccttatt
    11281 ttttgacaga tagtaaggcc gcttgtgccg ggaagcatga tgtccaatat ggcaaggtca
    11341 atctcagact tgtctataca ttccaatgct tctttggcgg tatagtattt gaaaaccgta
    11401 taattctcgt tttttaagta taattcaacc aaatcggcaa tttcatgttc atcatccaca
    11461 ataagtattt tatcgctcat agttatcacc ctttcacata agtttgccct taataattta
    11521 tcagattata gggccgaatt ta
//
LOCUS       DAXLPI010000079        11271 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXLPI010000079 DAXLPI010000000
VERSION     DAXLPI010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.079"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..760
                     /locus_tag="ACF3T5_001894"
     CDS             <1..760
                     /locus_tag="ACF3T5_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFD3632494.1"
                     /translation="EMETRVYTTLLEKGNLTGYEVSKVSGVPRSKVYNILEKLLKKNL
                     IVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTQNLGMIKEKDEEEMLWKVDGIEY
                     VLDKAEHLVKNAKESLLIQVWHENLTDSLLKTLQQAEKRVDKFVLILFSSSHEYDLPL
                     EKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDVTWTKNHAMVNLAKE
                     YVKHDAYTLKVIAESSEELKAKYGEDFEGIRKIY"
     gene            776..1654
                     /locus_tag="ACF3T5_001895"
     CDS             776..1654
                     /locus_tag="ACF3T5_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFD3632495.1"
                     /translation="MIRNILLALIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFITFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGVKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAAFLIFMGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFDEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYVLKWIVILVLLYS
                     AYTMLGSQLKKVSAAN"
     gene            1667..2647
                     /locus_tag="ACF3T5_001896"
     CDS             1667..2647
                     /locus_tag="ACF3T5_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFD3632496.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENLKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFDRASRFAEEMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVNKAKENNLGKLIY"
     gene            3169..3387
                     /locus_tag="ACF3T5_001897"
     CDS             3169..3387
                     /locus_tag="ACF3T5_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632497.1"
                     /translation="MNFFQNVFTDFVGDFKYLIMLAMLGGAAYIAFERKASKAIPLIL
                     VLAVAVWLVGDTSGVFNWILDRMRSWGR"
     gene            3389..3796
                     /locus_tag="ACF3T5_001898"
     CDS             3389..3796
                     /locus_tag="ACF3T5_001898"
                     /inference="COORDINATES: protein motif:HMM:NF024062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFD3632498.1"
                     /translation="MSEIRNWTKIWYIERVFYKLWDGVALPRPVTQTFAIWFGIGFFG
                     GIFRIFPFVMEHTITRQFFCPWLFAFLMTRKQFQGRKPYNYLASFLLFQCRSKESFHG
                     QWVKPIEKKEIKRGIRCVVRYGGEKDATTKTSA"
     gene            3771..6308
                     /locus_tag="ACF3T5_001899"
     CDS             3771..6308
                     /locus_tag="ACF3T5_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD3632499.1"
                     /translation="MRLPKLVPKIPFYYLEDNLVFGEEDRVYAYYEFPMYSYSFVGED
                     QAYQLQQAIMRLIRQSHSSHVRFFFCTNEEKIEKTIKSSKELVRSKGELKTIAYQHLE
                     GVQHILEELHGKYIQNVCWYVGIELVLEEIAPKEAHWLKEFRIAAKDFFHRSNARLFG
                     DFETIPNEKIQRYKRLENLLYNRISKYFRLRKTTPEDMVFLVEHLEGKKEQSLDEVMY
                     RPNSFKEETNTYTYHYDVLNLASAGITEKKNYLILEQGTEKEYITYLSLSRMTRDLVF
                     PFQSEIYYFLQSELDFPIDSWLDVNLLDNRLALAKVRGKKSDLSDIYESGMSVGKDVG
                     NNVREAYQSANELEQKLEDTREDLYVLSLNVRVSGKTYDEMLKRVSDVRNFFDVYSMY
                     LEVPCNVQLLLHFGSYPAGRRVIEDYRQYVGAEFIASLGIGATQQLGDGCGIPIGFNA
                     ETGIPVFIKPWLAAQGIKGTVTNTLAKALIGSLGGGKSLTENLLLFWSVLFGALAFCI
                     DPKSERSNWSEDLPYFAPYLKTVNITSEKENIGLLDPFQLMRRKKDQESLALDVLTYI
                     TGVSIRDEDRFPLLQRAVKTVSDFEGTKGLLLVIQELYAQEAATAQRLAKHIESFKEL
                     SIAGLLFGDGTNQQGLNLDAKCNLALVQDLTLPDSETVPEDYNSSEILSIAIMMSLAT
                     YALDFIKQNRKIYKAVGLDESWAWLNVAQGKILGDKLVREGRSRNAGIDFSTQNTDDL
                     GDEKMRSKIGLKFIFRSNDREEITKALSFCGISVTEENIQRVKNLENGECLFCDIHGN
                     IGILYVYYWFEDLFHAFDTRPPMEETYEEEEYKDEILEKLVEEIEVN"
     gene            6309..8330
                     /locus_tag="ACF3T5_001900"
     CDS             6309..8330
                     /locus_tag="ACF3T5_001900"
                     /inference="COORDINATES: protein motif:HMM:NF046089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFD3632500.1"
                     /translation="MKQWYSKVSFLITRRKLVFISLVIMMGVFFFHPSLVEAAGWIKE
                     TLGGKNAVYDFETYPLDNYNLDFFVDTSWNWLPWKWGEGISNTVIYGIFAVTNILWML
                     NVYLCYFLGFLVQEAFELDFLSELIGSLSHTIQNIAGMNRTGTLSHGLVPFFGGLLIT
                     LAGCSVVYQGIVKQHPTQAIQQAVTFLFTFICCGVFFMNTNTYLEKANGVQKEINSEM
                     LTIAKNLLPEQKEKPDAIKAIRENLFAIQIKQPWLVMQFGDSDIKTVGEERIINLLKE
                     SPYDEGEQRNKLVEEEVTKQKNGHLSVQKVYLRFGMVFLTLIVNIVISMSVLVLIVIL
                     IASQVLFLLFIGFLPVAMIFSLFPNSGSLLGNALQKVFQLLFTKMGILLILTITFSIS
                     HELNHLSKDKGMIWTSFLQVTLWLSVSTRVNELLGWMKIGGVQTRAGDRTGRFLRGMM
                     LGTMGQKMIRGIGENFVGGTAGGVAGQMISKSMSGQRSIASTPLIERVGKKVSAMTEL
                     PATIESRAEQWKRTIQYVPTNAQYKAQKMKYDYFSGRMNESERQTRARNLANERQKNF
                     YEARKDALQLEQQERKQRAMRSMAHQTGKTQVTSGKLQTRKSAMIGRTEQFPTIEKRS
                     NISNIAKNKKLSKQEHPIHERSTNRKIETPVRWTSTSRQNARKGRTKKE"
     gene            8477..9265
                     /locus_tag="ACF3T5_001901"
     CDS             8477..9265
                     /locus_tag="ACF3T5_001901"
                     /inference="COORDINATES: protein motif:HMM:NF024056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFD3632501.1"
                     /translation="MVFSVCFGVYNNLTSQDTHTIHETKVIDKQLKDMTGIESYVKNF
                     AFLYFTVSLESDQQINRSKAVESYLADGLQMDNEIDKDIETNVKVKNVSIWAIEEHES
                     KKHTFVVMFHVLLEIEKEFKERVYKVEVYRNELSYAIVKLPIVASNVKKATIKVDTRF
                     ETTSISRKEQEELEKFLYTFFRIYPKANAEELKYYGTTLLPIQQPLAFEELSNLQVAK
                     KEGRYEIHCNILYQDQETHLWFTMYYEMSMKQLENGNFRIEAIH"
     gene            9337..10356
                     /locus_tag="ACF3T5_001902"
     CDS             9337..10356
                     /locus_tag="ACF3T5_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFD3632502.1"
                     /translation="MSSGESKHMDSAVGVTHTGEYIETLPIFSEIKGRGPITDEHAQA
                     AVGAAVKYQLLPSVILSQLGYESAYGQSQSGRNDTNFFGITWFPGCPYPQGSARGIGG
                     SEGGFYMKFPNLNACYDYYGFMVASQSNFNQCVGNKDPGSCLLILGRGGYAAAGITEG
                     SSYYQGAISIIKSYNLTEYDAFAIKHWNSLPIKKTSLIAGKSKGNAKVLDRIIGRPVN
                     NGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGEDYNWATYGWQVIMNPKPSDLQAGDV
                     VNWQAGGALSPGIYGHTGIITDVSNSGQSFSTVEQNAEQGRIMARYQRGYNQAKIRSL
                     VRKVK"
     gene            10407..10919
                     /locus_tag="ACF3T5_001903"
     CDS             10407..10919
                     /locus_tag="ACF3T5_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFD3632503.1"
                     /translation="MKKYYFFFLSCFCLFGLTSCGWIFSEEKTEVSLISKEKMIQAES
                     SSKESKRNQEDKTECNISEEIQYLLKNFGRKWVNYTDIYERNQSVKNYLTDRCIKENG
                     IDNDPHIQYKTIGRIHTITQDVIQSDQYLLFGEETTNEITRFVLLQIKVDQEVQKIDK
                     IKIYYVRSAY"
     gene            complement(11038..>11271)
                     /locus_tag="ACF3T5_001904"
     CDS             complement(11038..>11271)
                     /locus_tag="ACF3T5_001904"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFD3632504.1"
                     /translation="ETPVTPENPEPDKPVSLKNTNSKILISEKNVSNTNSKSNNTLPQ
                     TGEKESSGVLAGLVILLASIAVGIKLNLKRKNI"
BASE COUNT         3763 a         1702 c         2421 g         3385 t
ORIGIN      
        1 agagatggaa acaagagtgt ataccacact attggaaaaa ggcaatctta caggatatga
       61 agtaagtaaa gtctcaggtg tgccacgctc gaaagtatat aacattttgg aaaaactatt
      121 aaaaaagaat ttgattgtcg tcaataaatc agaacctaaa ctttatcatg ccatctcggc
      181 aaatgagttt ttagagaaat tagaaaaaag tgttaaaaat gatctctctt ttcttactca
      241 aaatctaggg atgatcaaag aaaaagatga agaagaaatg ctgtggaaag ttgatggcat
      301 cgagtatgtt ctggataaag cagagcattt ggtgaaaaat gcaaaagaaa gtttgctcat
      361 ccaagtttgg catgagaacc tcacggattc actgctaaag acattgcagc aggctgaaaa
      421 aagagtcgat aaatttgtac tgattttatt cagttctagc catgaatatg atcttccctt
      481 agaaaagtat tacattcatg gttttgaaac agacaaactc gctgactttg gggcaagatg
      541 gataaacatc gtcgccgatg agcaagaagt cgtgtttggc accattaacg aagaactgca
      601 atcaaccgat gtcacttgga caaaaaacca cgccatggtc aatctagcaa aagaatatgt
      661 caaacatgat gcatatacac taaaagtgat tgcagaatct tctgaagaac ttaaagcaaa
      721 atatggtgag gactttgagg gaatcagaaa gatttactag taggaggaaa tttttatgat
      781 tcggaatatt ttgctggcac taattgtcgc aatcaatcta tattttatta tttactttct
      841 aagagacctt ttcaaaaaca aacaaaattt caaagaagaa ccaggggata tgcgtctgtt
      901 gcccttcacg tcatttatca ccttctttct ttcaacattt ggcgtttccg actttgctat
      961 cggaaccgtc ctttatccaa agttgaaatg ggtcagcatg aaaaaactac ccggaacgtt
     1021 gaatacccaa tgtgtcgtac cggtagccgt catggcattg tcttatatca ctgccatcaa
     1081 cgtaggggtc aagacactag ctgtttgtat tatctgccaa attatcggtg catatttagg
     1141 accaagattt gtcgtgaaat taccagaaaa aacgatcaaa ctatttgtcg gcataggatt
     1201 ggtcattgct gcattcttaa tttttatggg acaaatgaat tggattccct caaacggaac
     1261 agccagtgaa ttgtatggcg gaaaattaat cttggcagga tttttactct ttgtctatgg
     1321 cgcattgaac aatattggca ttggttctta tgcgctaacg atggtaactg tctacttgct
     1381 aggcttgaat ccagttgcag cctttcctat tatgatgggg gcttgtacat tctcagtacc
     1441 gatcggcagt gtccaattca ttaaatttga tgaatacagc agaaaaatca cacttttcac
     1501 ttcaaccttc ggtgtattag gggtattggt cgctgtattc ctagtcaaat ctttagatac
     1561 ctatgtcttg aaatggatcg taattttggt cttactatac agtgcataca caatgttggg
     1621 cagtcaatta aaaaaagttt cagcagctaa ttaaggagcg attcaaatgt tatttcttaa
     1681 aaaagaagat attatcaaaa gtttctcaat gagagaagcc atcgatgcag acaaaaaagc
     1741 attgtcattg tattcagcag gaaaggcaag tgtgcctctt cgcaccaata tcgatgtgcc
     1801 aaaaagcaac ggacaaagct tatatatgcc agcttatgta gaaggtggcg aaggtgcgtt
     1861 aggtgtcaaa atcgtttccg tgtatcctga aaacctcaag aaaaacttac caagtgtacc
     1921 agccacgatg atcgtccttg atccagaaac gggtatggta tccgcttgtt tggatggtac
     1981 ctatttgaca caattaagaa ccggcgctgt tcaaggtgca gcaacagaac ttcttgcaag
     2041 agaagatgct aaaatcggcg cactgatcgg aaccggcgga caagcacagt cgcaattaga
     2101 agcgatgttg accgttcgta aattagaaga agttcggatt tttgatattg attttgaccg
     2161 tgccagccga tttgctgaag aaatgatgca acagttttct gtaacgatgc gcccaacaaa
     2221 aaccagtcaa gaatgcgttg aaggagcaga tatcattaca agtgtgacca cttcaaaacg
     2281 tgcgacattc tctgctgagt gggtcaaaaa aggtgcgcac atcaacggag tcggtgctta
     2341 cacaccggaa atgtgtgaaa ttccacgaga aataatcaaa gccgcagatg ttgttatttt
     2401 tgataccatg gacggtgttc taaaagaagc tggggacttc atttcaccgc tgcaagatgg
     2461 gtatattcaa agagacagtt atcatgggga actaggccaa ctgatcaatg aagaattgat
     2521 cggtcggaca agtggagaac aaatcacaat tttcaaaaca gttggttcgg ctgtattaga
     2581 tgtggtagtt gcgacagaaa tcgttaataa agctaaagaa aataatttgg gaaaattgat
     2641 ttattaaata aaaagaccgg aagtaaacct ttaattagta catttataaa aagagaacaa
     2701 aaaagactgt aatcaaaatg cgattacagc ttttttttgt tttatttggg aggagataga
     2761 tcgtgtttat gactgtatcg gatcatttaa aggtacttgg ttttatccct ttatctagta
     2821 gagggaagta tcaattgtat ttgttagatt taccacccta gcttttcact gatgattgaa
     2881 ggaacatacg ataaaaaaag tggtaagtat ttttatgatt tttattctat gaaacttgat
     2941 cctaaaacaa agaaaagtac gcagcaccgg atttattgta cgcatgttcc tgtaaaagta
     3001 atgtatcgtc gtgtgagaaa ttatttatac ttcttacgta aaaaacagca atcaaggaac
     3061 agaaaaaatg cataatgagg aatatcaagt gaaattttta tcagttttgc tctgtggtat
     3121 tgtgtattgt cattagttca atgagtatta acagggagga aaaagcaaat gaattttttt
     3181 caaaatgtgt ttaccgattt tgttggggat tttaagtatt tgattatgtt ggcgatgctt
     3241 ggtggtgcgg catatattgc ctttgaacgg aaagcaagca aagccattcc attgattctt
     3301 gtgctagcag tagctgtttg gttagttgga gatacgtctg gcgtattcaa ttggattttg
     3361 gatcgtatgc ggtcatgggg gcgataaaat gagtgaaata cgcaattgga caaagatttg
     3421 gtatatagag cgagtgtttt ataaattgtg ggatggggtc gcgttacctc gtcctgtaac
     3481 acaaacattt gcgatatggt ttgggattgg cttttttggg gggatttttc gaatttttcc
     3541 atttgttatg gaacatacga ttactcgaca atttttttgt ccgtggttat ttgctttttt
     3601 aatgacacga aaacaatttc aaggaaggaa gccttataat tatttggctt cttttctttt
     3661 atttcagtgt cgttcaaaag aatcctttca tggccaatgg gtaaagccaa tagaaaaaaa
     3721 agaaatcaaa cgaggaattc gttgtgtagt acgttatgga ggtgaaaagg atgcgactac
     3781 caaaactagt gcctaagatt cctttttatt atttagaaga caatttagtt tttggagaag
     3841 aagatcgtgt gtacgcttat tatgaattcc caatgtattc atatagcttc gtgggagaag
     3901 atcaggcgta tcaactccaa caagcaatca tgcgtctgat tcgtcaaagt cattcttcgc
     3961 atgttcggtt ctttttttgt acaaacgaag aaaaaattga aaaaacgatt aaatcctcaa
     4021 aagaattggt gcgttctaaa ggggaattaa aaacgattgc ctaccaacat ttagaaggtg
     4081 ttcagcacat tttagaagag ctacatggaa aatatattca aaatgtttgc tggtatgttg
     4141 ggattgagct ggtattagaa gaaattgctc ccaaagaagc tcattggtta aaagaatttc
     4201 gtattgccgc aaaagatttt tttcaccgat ccaatgcccg tttgtttgga gactttgaaa
     4261 ccatcccaaa tgaaaaaatt caacgatata agcgattgga gaatttattg tataaccgga
     4321 ttagtaaata ctttcggtta cgaaaaacaa caccagaaga catggtgttt ttagtggaac
     4381 atttagaagg aaaaaaagaa caatccttag atgaagtgat gtatcgacca aatagcttta
     4441 aagaagaaac gaatacgtat acgtatcact atgatgtatt gaatttggcc agtgcaggaa
     4501 tcacagaaaa gaaaaattat ctaattttag aacaagggac agaaaaagaa tatatcacgt
     4561 atttatctct ttcacgtatg acccgtgatt tggtatttcc atttcaatca gagatttatt
     4621 attttttaca atcggaattg gattttccaa tcgattcttg gttagatgtg aatcttttag
     4681 ataatcgttt ggccttggcg aaagtacgtg gcaaaaagag tgatttatct gatatttatg
     4741 aaagtggtat gtcggttgga aaagatgtag gaaacaatgt acgcgaagct taccaatcag
     4801 ccaatgagtt ggaacaaaaa ttagaagata cgcgagaaga tttgtatgta ttatcactaa
     4861 acgttcgcgt gtcagggaag acctatgatg aaatgttaaa acgtgtgagt gatgttcgca
     4921 atttttttga tgtctattct atgtatttag aagtaccgtg caatgtacag ctgcttttac
     4981 attttggaag ttatccagca ggaaggcgag tgattgagga ttatcgtcaa tatgtaggcg
     5041 cagaatttat tgctagtctt ggcattggtg cgacacaaca actaggagat ggctgtggta
     5101 ttccgattgg atttaatgca gaaacaggaa tcccagtgtt tattaaacca tggttagcgg
     5161 cacaaggaat aaaaggaacg gttaccaata ctcttgccaa agcattaatt ggttcgctag
     5221 gtggtgggaa atcattaact gagaatctct tgttattttg gtcggtgttg tttggcgcct
     5281 tggctttttg tatcgatcca aaatcggaac gttcaaattg gtccgaagat ttgccatatt
     5341 ttgcccccta cttaaaaaca gttaatatta ccagtgagaa agaaaatatt ggtttacttg
     5401 atccatttca attgatgaga agaaaaaaag atcaagaaag tttagcattg gatgtgctca
     5461 cctatatcac gggtgtaagt attcgggacg aagaccggtt tcctttgtta caacgggcag
     5521 ttaaaactgt ttctgatttt gagggaacaa aaggattact tctagtaatt caggaattat
     5581 atgcacaaga agcagcgacc gcacaacgcc tggcaaagca tattgaaagc ttcaaagaac
     5641 tatctattgc aggcctatta tttggagatg gcacgaacca acaagggtta aatttggatg
     5701 ccaaatgtaa tctcgcactg gttcaggatt taacgttacc agatagtgaa actgtacctg
     5761 aggactataa ctcttctgaa attttaagta ttgcgattat gatgagtttg gctacgtatg
     5821 cgctagattt tattaaacaa aatcgaaaaa tttataaagc ggttggctta gatgaaagtt
     5881 gggcgtggtt gaatgttgcg caaggaaaaa ttttaggaga taaattggtt cgtgaaggaa
     5941 gatctcgcaa tgcagggatt gatttttcaa cacaaaacac agatgattta ggtgatgaaa
     6001 agatgagaag taaaattggg ttgaaattca tttttcgttc gaatgatcgc gaagaaatta
     6061 ccaaagcatt aagcttttgt ggaatatctg tcacagaaga aaatatccag cgtgtaaaaa
     6121 atctagaaaa cggtgaatgt ctgttttgtg acatccatgg taatattggc attttgtatg
     6181 tgtattattg gtttgaagat ttattccatg catttgatac acgaccgcca atggaagaaa
     6241 cctacgaaga ggaagaatat aaagacgaga tattagaaaa actggttgaa gaaattgagg
     6301 tgaattagat gaaacagtgg tattcaaagg tttcattttt aattactcgc cggaaactgg
     6361 ttttcataag tctagtcata atgatgggtg tttttttctt tcacccatca ttagtagaag
     6421 cagctggttg gataaaagaa accctaggcg gcaaaaatgc cgtatatgat tttgaaacat
     6481 atcctttaga taattataat ttagattttt ttgtggatac gtcgtggaat tggttgccgt
     6541 ggaaatgggg tgaaggaatt tctaatacag ttatttatgg gatatttgcc gttaccaata
     6601 ttttatggat gttaaatgtc tacctgtgtt actttttagg gtttcttgtc caagaagcct
     6661 ttgaattaga ttttctttca gagttgattg gttcgttaag tcatacaatt cagaatattg
     6721 ctggaatgaa ccgtacaggg acattgagtc atggattagt ccctttcttt ggtggattgt
     6781 taattacttt agcaggttgt tcagtagttt atcaaggaat agtgaaacaa caccctacgc
     6841 aagctattca acaagcggtg acatttttgt ttacttttat ttgttgcggc gttttcttta
     6901 tgaatacaaa cacatattta gagaaagcta atggggtaca aaaagaaatc aatagtgaaa
     6961 tgttaaccat tgccaaaaat ctgttacctg aacaaaaaga aaaaccagat gcaattaagg
     7021 ccattcgtga gaatttattt gccattcaaa ttaagcaacc ctggctagtg atgcaatttg
     7081 gggatagtga tataaaaacg gttggtgaag aacgtataat caatttatta aaagaatcac
     7141 cttatgatga aggagaacag cgaaataagc ttgttgaaga agaagtaacc aaacaaaaaa
     7201 atggccatct ttcggtacaa aaagtgtatt tgcgttttgg tatggtattt ttaacgctca
     7261 tcgtcaacat tgtcattagt atgagtgttt tggtattaat cgttatctta attgcgtcgc
     7321 aagtattatt cttgttattt ataggattct tacctgtagc gatgattttc tcgttatttc
     7381 caaattcagg atcgttatta ggtaatgcct tacagaaagt ctttcaatta ttgtttacaa
     7441 aaatgggtat tttactcatt ttaaccatta ctttttctat tagtcatgag ttaaaccact
     7501 tatccaagga caaaggaatg atttggacca gtttcttaca ggtgacgtta tggttatctg
     7561 tctcgacacg tgtcaatgaa ttattaggtt ggatgaaaat tggtggcgta caaacacgag
     7621 ctggggatcg aactggacgt ttcttacgtg gcatgatgtt aggtacaatg ggtcaaaaaa
     7681 tgatacgagg aattggtgaa aattttgttg ggggtactgc cggaggtgta gctggccaaa
     7741 tgatttccaa atctatgagt ggtcaacgaa gtatagcttc tacacctctt atagagcgag
     7801 taggaaaaaa agtttcagct atgacagaat taccggcaac gattgaaagt agggcagaac
     7861 aatggaagcg gacaattcag tatgtcccaa ccaatgccca atacaaagca caaaaaatga
     7921 aatacgatta tttctctggg cgaatgaatg agtcggaacg acaaacacga gcgcgtaacc
     7981 ttgcgaatga gcgacagaag aatttttatg aagcacgaaa agatgcattg caattagagc
     8041 aacaagagag aaaacaacga gcgatgcgtt caatggcgca tcaaacagga aaaacacaag
     8101 tgacttctgg taaattacaa acacggaaaa gtgcaatgat tggtcgaaca gaacaatttc
     8161 ctacgataga aaaaagaagt aacatttcaa atatagctaa aaataagaaa ttgtcgaagc
     8221 aggaacatcc gattcatgaa cgaagtacga atcgaaagat tgaaactccg gttcgttgga
     8281 cttcgacttc tcgtcaaaac gcgagaaaag gtagaactaa aaaagaataa ggaggagggc
     8341 tatgtttcct cggaagaaaa aactgtctat tctctctgaa caagaaacaa gactaaaagg
     8401 aaagaaagac aagaaaaaag aaagctataa gggagcgcga aaaaggcgct tccttttttt
     8461 tgtaggctgg agctttttgg tttttagtgt ttgttttgga gtttataata atttaacttc
     8521 tcaagatacg catacaatcc atgaaacaaa agtcattgat aaacaattaa aagatatgac
     8581 tggaatagaa agttatgtga aaaattttgc atttttgtat tttacagtga gtcttgaaag
     8641 cgaccaacaa ataaatcgat caaaagcagt agaaagttat ttagctgatg ggctgcaaat
     8701 ggataatgaa atagataaag acatagaaac gaatgtaaaa gttaaaaatg tctcgatttg
     8761 ggcaatagag gaacatgaat caaaaaaaca tacatttgtg gttatgtttc atgtcttatt
     8821 agagatagaa aaagaattca aagagcgtgt gtataaggtg gaggtatatc gaaacgaatt
     8881 atcatatgcc atagtgaaat tgcctattgt agcgagtaat gttaaaaaag caacgattaa
     8941 agtagacact cggttcgaaa caacgagcat ttctcgaaaa gaacaggaag aattagagaa
     9001 attcttatat accttctttc gtatttatcc taaagcaaac gcagaagaat taaaatatta
     9061 tgggacaact cttttaccaa ttcaacagcc tttagccttt gaagaactat cgaatttgca
     9121 agtcgcaaaa aaagagggta gatatgagat tcattgtaat attttgtatc aagatcaaga
     9181 aacgcacctt tggtttacca tgtactatga aatgtctatg aaacaattag agaatgggaa
     9241 ctttcgaatt gaggcgattc attagtgaaa aagatttttt tatttagtgg catctgtatg
     9301 atgagttgtc tgtttttatt atttgcgata attagcatgt ctagtggtga atcgaagcac
     9361 atggattcag cagttggtgt tactcatacg ggagaatata tcgaaacctt acctattttt
     9421 tcagagatca aagggcgtgg tccgataaca gatgaacatg cccaagcagc agttggtgca
     9481 gctgtaaagt atcagttatt accgagtgtg attctttctc aattaggata tgaatcagct
     9541 tatgggcagt cacaatctgg aagaaacgat acgaactttt ttgggattac gtggtttcct
     9601 ggttgtccct atccacaagg ttctgcacgt gggattggtg gttctgaagg tggtttctat
     9661 atgaagtttc cgaacctgaa tgcttgttat gactattatg gatttatggt ggcgagtcaa
     9721 agcaacttta atcaatgtgt gggcaataaa gatcctggaa gttgtttatt gattctaggt
     9781 cgtggaggtt atgcggcggc aggtatcacc gaaggcagtt cgtattatca aggcgcaata
     9841 agtatcataa agtcctataa cttaacggaa tatgatgcct ttgcgatcaa gcattggaat
     9901 agcctaccaa tcaaaaaaac ttcgcttatt gcaggaaaaa gtaaaggcaa tgcgaaggtt
     9961 ttggatcgga tcattggacg acccgtaaat aatggggaat gttatggtgg tacggcatgg
    10021 tatgtatctc aactaggtgg cccacagtta atgggttctg gacacatgtt tgcgatgtta
    10081 ataggggaag attataactg ggcaacctat ggttggcaag tgatcatgaa tccaaagccc
    10141 agtgacttac aagcaggaga tgtggtcaat tggcaagcag gtggtgcatt gtctcctgga
    10201 atctatggcc acacgggtat cataacagat gtctcaaata gtggacaaag tttctccacc
    10261 gtagaacaga acgccgagca ggggcgaatt atggcacggt atcaacgcgg ctataaccaa
    10321 gcgaaaattc gaagtctggt tcggaaagtg aaataaaaga attctttcga tgaaaatttt
    10381 tagtagaaaa gggagaagga tcgattgtga aaaagtatta ttttttcttt ctaagttgtt
    10441 tttgtttgtt tggacttact agttgtgggt ggattttttc tgaagaaaaa acagaagttt
    10501 ccttaatatc aaaagaaaag atgatacaag cagaaagttc aagtaaagaa agcaaaagaa
    10561 atcaggaaga caaaacagaa tgcaatattt ctgaagaaat ccaatatttg ttaaagaatt
    10621 ttggaagaaa atgggtgaat tacacggata tatacgaacg aaatcaatct gtaaaaaact
    10681 atttaaccga tcgttgtata aaagaaaatg ggattgacaa cgatccacat atacaatata
    10741 aaacaattgg ccgtattcat actatcacac aagatgtaat ccaatcggat caatatttat
    10801 tatttggaga agaaacaaca aatgagataa ctcggtttgt gttacttcaa atcaaggtgg
    10861 atcaagaagt gcagaagatt gataaaatca agatttatta tgtaagaagt gcatattaaa
    10921 ataaaaaagg aattgtggaa attgtttgta attaaatcga acagtgatta tttattttaa
    10981 attgaaaaca gagataggat gaatacttat tctatctctg ttttcttttg gactaatcta
    11041 gatatttttt cgttttaaat ttaatttaat acccactgca atagaagcta ataaaataac
    11101 taatccagct aaaactcctg atgattcttt ttcaccagtt tggggaagag tattatttga
    11161 ctttgaatta gtatttgaaa cattcttctc tgatattaaa attttagagt tagtgttttt
    11221 taaagacact ggtttatcag gctcagggtt ctcaggcgtt actggagttt c
//
LOCUS       DAXLPI010000080        11202 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXLPI010000080 DAXLPI010000000
VERSION     DAXLPI010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.080"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(105..767)
                     /locus_tag="ACF3T5_001905"
     CDS             complement(105..767)
                     /locus_tag="ACF3T5_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632505.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(780..2282)
                     /locus_tag="ACF3T5_001906"
     CDS             complement(780..2282)
                     /locus_tag="ACF3T5_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632506.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            2830..4509
                     /locus_tag="ACF3T5_001907"
     CDS             2830..4509
                     /locus_tag="ACF3T5_001907"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFD3632507.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4636..5115
                     /locus_tag="ACF3T5_001908"
     CDS             4636..5115
                     /locus_tag="ACF3T5_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFD3632508.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5334..6014
                     /locus_tag="ACF3T5_001909"
     CDS             5334..6014
                     /locus_tag="ACF3T5_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFD3632509.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            6022..7353
                     /locus_tag="ACF3T5_001910"
     CDS             6022..7353
                     /locus_tag="ACF3T5_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFD3632510.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7368..8330
                     /locus_tag="ACF3T5_001911"
     CDS             7368..8330
                     /locus_tag="ACF3T5_001911"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFD3632511.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8441..8779
                     /locus_tag="ACF3T5_001912"
     CDS             8441..8779
                     /locus_tag="ACF3T5_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFD3632512.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            8858..9565
                     /locus_tag="ACF3T5_001913"
     CDS             8858..9565
                     /locus_tag="ACF3T5_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFD3632513.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9668..11149)
                     /locus_tag="ACF3T5_001914"
     CDS             complement(9668..11149)
                     /locus_tag="ACF3T5_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632514.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3565 a         1948 c         2337 g         3352 t
ORIGIN      
        1 ataaaaacca cttattacta attgaatcaa gcaaataaaa aatccgaacc atatctgcga
       61 gacagatatg gttcggattt tttatttgat cattatcctg ttttttaaac ttcgattttt
      121 tgaaattttt tattttttag ttcaaagact tcatcacagc tttttgctac ttcaatctca
      181 tgagtcacga tgataataca gcgattttgt tcatgggcga tttcttggaa aagtttgacg
      241 atatcttgtg aagttgtttc atctagattt ccagtaggct catccgctac gattagttga
      301 tgttcacagc agatagcacg aacgatagcg actcgctgct gctgacctcc cgaaagtttg
      361 gtcacctgtt tttttgctaa tatttcatca attcctacac gagccagtcg atctaaagca
      421 acttgtcgat aattctcttg cttggtctta gtgatttcca ttgctgctat gacgttgtct
      481 agagccgata aataaggaag caagttgtaa gcttggaaaa taatcgagac atcattttta
      541 cgataacttc ttaacccgat tttgttcagt gaacgatctt gatagtatac ctttccttct
      601 tttggtgcat ctagtccaga aataagcgaa agaaaagtcg tttttccaga accacttgct
      661 cctaaaatag cgtacatttt tccagcttcg aaattcagtt ctacatcttt ataaagcgca
      721 tcttgcggat cttgatacca aaatcccaat tgttctgtcc gtaacatcaa attcctcctt
      781 tagtttaata ggatttttct tgggtttaat cttaatatcc ctatagaagc taaaaagaca
      841 gaaaacagac tgattccaaa tgctactccg gctaatagtc ccaattgtgc aggtttgaca
      901 ttgatttcca attcgctgac ttgatcagaa acagcaaatg gattattcgt ggttccttgt
      961 tgaccatcgc gttgaccgcc ttgttcttgt ctgttacctg gcatttgtcc accgccagga
     1021 gcttgctggt tttgtgtcgt ggtttctgtc tgttggtcaa gtagctgctg tcccacggca
     1081 ttcgcgacga cgtttccgct aagcgaagca actcctaatg caagaatcat acaaatagcc
     1141 acttcggtaa acaattggag aatgatttta cttctggatt ctcctaaaga tagcaagacg
     1201 cctaactcat gccgacgctc ccgaatggta atcataatga tcagagtaag tatgataata
     1261 ccggctacag tcactagtaa aacaacattt ttagaaaaac ttgccacgtt gtttaaaggt
     1321 tccaacattg actgatacat ggaatcattt gattgtaagc taaatgtatc tgtgtcgatc
     1381 agcttctcag cttctttgag aaactcatcc attttatctg gatcagatag agtgtaaata
     1441 gcagaatcta tcgtgttatc gtcagatgtc ccatttagtt catttgcaaa agtgtaagaa
     1501 gtgtaaagtg tattcgacgg attcatgaag ttgaatttca tacccatact gccgcttgtt
     1561 tcgctggatt cataaattcc tttgatcgtc atttcataag tggtatcttc atcttcagta
     1621 ctcgtgattg tgaaagtatc tccgactgcc aggtcatttg tttctgctag agtagaatca
     1681 atgactacat tgtttgtgcc tttgtcgctg tcatctattc cttcaccatc cgtgattttt
     1741 gctgttccat cagaaaaatt actattttgt gcagttgctg acacaccact gatttgaaag
     1801 tctgcttgag tcatttgcgg attcccgccc attcctccag caggttcacc gcctgcatca
     1861 gtagtttctg aactgtcttc acttgaatca gaactagtga tggcagttat tccatctttt
     1921 gccatagctg aggcggaact ttcaaaggaa taactcttta cattatctaa ttcagctatt
     1981 ttttccgcgt ccgttaagga aactggtgta agagaaaaac tgcctggatc tggacgttct
     2041 tcagtggtag aaccgcttga agaagtttgt tcctgttttt taaatgaagc ttctcgattg
     2101 gcacttaaag tcactgttgc tccaacttct ttttgtgctt gattcgctgc ttcttctgca
     2161 gcgcttcgaa tcgtgaggcc agctagcaca aaaataagaa tggctgtgaa cacggcaaac
     2221 aataacaatg aacgtcccca ttttactttt gtacttttcc atgcacgttt cataaaattc
     2281 atattttgca tccctcctag tagatgcatt tgataataga caacctatct taaagctagc
     2341 ttaaatctta tgctgtttgc acaccatcaa catgaggttc tgtatatata aaaagtcact
     2401 atagttattg caaaaaatgt ggtaaaatga aaaaagtatg tttcaaaccg acgaagaaga
     2461 aatgaaagag gaatagcaaa agaaatccta gatgctacag agggattttt acgtgcatag
     2521 ttgttctaac aattgctgat actagcgatt tagtttattt gttgtataat aatggggaac
     2581 agctgaatac ttatataagt attctggcta tagcgtttcg caaaagatcc attttgttaa
     2641 tgaagtatat ccaaaataaa atgcccatcc aactgtcaga acgcggatgt gctggtaatt
     2701 ttcctgctga ttctccataa gttgagaaat agtgattttg catatgcttc attggaatgg
     2761 ctcatatatt tagataaaga agaaaatcga catctttaaa cttggatgtc ataagaagag
     2821 gtgaagtttg tgagtaccat taaaattatc ccattaggcg gcgttcgtga aaatggaaaa
     2881 aacatgtatg tcgcagaagt gggcgaggat atttttattt tagattgtgg gctaaaatat
     2941 cctgaaaatg agttgttggg gatcgacgtc gtaatccctg attttacgta tttagaagaa
     3001 aacattgatc gtgtagctgg tgtcttttta acacatggac atgcagatgc gatcggggca
     3061 cttccttact tgttgtcaaa aatttctgta ccagtattcg ggacagagtt gacgatcgag
     3121 ttagcaaaat tgaatgtcag tcgtaatgaa gcagccaaag gatttaagga tttccatgtg
     3181 gtagatgagc atacagaaat agactttggt gaaacagtag tgagcttttt ccgtaccact
     3241 catacgatac cagattcagt ggggatcaat ctgaaaacaa aagaaggtag tatcgtttat
     3301 acgggagatt ttaaatttga ccaaacggct atcccaatgt atcaaacaga ttatgcacga
     3361 ttggcggaaa tcggcaaaga aggagtctta gccttactaa gtgaatcggc aaatgcagaa
     3421 aatcctcagc ctgtagcttc tgaagcccaa atagcagatg aagtatttga tacgatcaaa
     3481 tattgggaag ggcgtatcat cgtcgcttgt gtagcaagta atttgcaacg agtgcaacaa
     3541 gtattggatg cagcagaaaa agcacaacga aaagttgtct tgactggtca agatttcgaa
     3601 cgaattattc gcacagcgat gcgtttagaa aaacttcaat taccaagtga agatctgctt
     3661 gttacgttga aagacatgaa aaactatgat ccagaagaac tgatcatttt agaaactggc
     3721 cgaatgggtg aacccatcaa gtctctgcaa aaaatggcaa acggtacaca ccgtaatgtc
     3781 aagattgaag aaggagattt ggtttatatc actactacgc caagcatcgc gatggaaacc
     3841 atcgtggcga aaacggaaga tatcatttat cgagctggtg gaacagtgaa gttgatctct
     3901 gagaatatgc gcgtttctgg ccatgccaat cctaatgact tgcaattaat gatcaatttg
     3961 atgaaaccaa catattttgt gccagttcaa ggagagtatc gtgaattagc tgctcatgca
     4021 gatttggctc atgcagtagg gatgccatac aaaaatatct atattactgg acgcggagac
     4081 gtgctggaat ataagaacaa gcgtatgagt gttgcaggaa ctacaagtgc agaaaatgta
     4141 atgattgatg gattaggcat aggtgatatc ggaaacattg ttttacgtga tcgaaaaatc
     4201 ttgtcagaag acgggatttt cgtagcggtt gtcactatca gtcggcgaga aaagaaaatc
     4261 atttcaaaac ctcaaattac ttcacgggga ttcgtttacg taaaagctag ccgtgatctg
     4321 atgaaagaaa gcagcaatat gattgaaaca atcgttgaga aacatttaga aagcaatgat
     4381 tttgagtgga gcaaattaaa gcaagacatc cgagatcaat taggccgtta cttatttgaa
     4441 caaacaaaac gccgcccagt gatcttgcca gtcattatgg aagctactca acgtcgaaat
     4501 aaaaaataaa caaaaagagt aagatgaacc tctctataga gtgctcgtct tgctcttttt
     4561 ttgcgaaaaa caaataattt ttgaatacag catccctcgc tttataataa acgatagaat
     4621 aaagggagtg aatcaatgat gagtcaaaca aaagtcgaac gttatttaga agaacaaaag
     4681 atcagctatc aaccacttga ttttagtcat gtggtagaag aatctttcac agaagaacta
     4741 caaaaaagag ggattgatcc agctttgata tgtaaaactc tcgtagtgaa aggaaacaaa
     4801 actggggtga tcatcgcatt gattcctttg aacgatcact tagattacaa aaaaacgaga
     4861 caagtgacga aagatcgtaa agttggtttt cctggaatgg attttgtatt aatgcataca
     4921 ggatatcccc acggagcaaa cacccctatt ggaataaaac tcgcacatcc tgattattgt
     4981 tttttagcag acagttcttt aaaagaaaaa gaagaagtaa ttgtgtcaag cggtgaaatc
     5041 gggagaagcc ttcggataaa agtagccgat ttagaaaaga tcgtccatcc tatttacgct
     5101 gatttaatta gttaagggac gagaagagaa gctaagcaga gcagagacct caaacttgag
     5161 attatatctg cttggtactt ggtgcatcct gtattatctg ttacaattgc tttgtacata
     5221 ctgtactatc aatccaatga aaatatattt agcagctata ttttttcatt gatgtgcaag
     5281 tgatgaaatc gtttttaata aaaaagatac agagaaaacg cgaggtgtaa aatatgaaaa
     5341 ttacgtatca tggacattcc tgtttaacaa ttgaaatgga aaatggccaa cgcgtgatga
     5401 ttgacccgtt tattacagga aatccattga cagacttaac tgcagaagaa gtaaagacag
     5461 attggttgct gattactcat ggtcatagtg accacatagg agatatgatt cctattgcaa
     5521 aagaaaatca agccacaatt atcagtattg ttgagattgc tgattatgcg aacagcagag
     5581 gagtagattc attcggtatg aatatcggtg gaaaacatgc gtttccattc ggcgtggtaa
     5641 aattcgtaca tgcacagcat agttcaagct atgaagtaga cggtatcgtt cagtatatgg
     5701 gcgagcctag cggaattatt attcaagcag aaggaaaaac gatttatcat gcaggggata
     5761 ctgcttattt cagtgatcta ggattgcttg cagaagaatt cgatattgat gtcgctttct
     5821 tgccgattgg cgataactat acgatggggc cagaagatgc agcaaaggct gcgcaaacaa
     5881 ttcgtgccaa gaaagtagtt ccaatccatt acaatacttt ccctgtcatt gaacaagatc
     5941 ccgaatcttt tgtagagtta ctggaagata gtgaaggaaa aataatgagc gtctcggaaa
     6001 cgctggaagt gtaggcagaa aatggcgaca aaacatgatt tgattttaga atatattgaa
     6061 agtttacctg ttggcaatcg aatttctgta agaagcatcg caaaagaatt gaatgtcagt
     6121 gaaggaacag cgtaccgtgc aatcaaagat gcagaaaatg tcggactagt ttcaacaatt
     6181 caacgagtag ggaccatccg tatcgaacgc aaattaaaaa aacatattga acgtttgaca
     6241 tttggcgagg tcgtcaggat cattgaaggg gacgttttag gcggggcggc aggattagac
     6301 aaggtcttga ataaattcgt catcggcgcg atgactaaac atgccatgga acgctatatt
     6361 acgccaggat cactaatgat cgtcggcaac cgggcggaag tccaaaaatt agcattggaa
     6421 gatggcgccg cagtattgat cacaggagga ttcaatacga ctgctgagat tgcaaagcta
     6481 gctgatgatc ttgaaatgcc gatcttacgc acctcttatg atacatttac agtcgcaacc
     6541 atgatcaacc gagcgttaag cgaccaattg atcaaaaaag atatcatgct tgtcagtgat
     6601 atctatatgc cagctgaaaa gactcattat ttgcagcaga cagatacgat ttctgattat
     6661 cagcgtttgt cagaagaaac gcagcattcg cgttttccag tcgtcaaccg ccatcatcgt
     6721 ttgatgggga ttgttacggc aaaagatgtt ttagggaaat cgccaaacca gatcatcgat
     6781 cgggtgatga cgaaagaacc aatcagtgta aagaaaacaa tgagtattgc ctctgttagc
     6841 catcaaatga tctgggatgg gctagaagtc atgccggtcg tttccgatga cttgacattg
     6901 gaaggcttga ttacccggca agatgtaatg aaagcgatgc agcttgtcca gcgacaaccg
     6961 cagattgcag atacgatttc ggatcagata tctggaaata tccatacgat tgatactgac
     7021 cgtgaaggaa atcgtttgga aaaccctaag ttcaaattcg tagtagcacc acaaatggtc
     7081 aatggcgttg gaaccatttc ttttggcgta cttagcgaaa tcatttctga tgtcgcacaa
     7141 aaaacaatgg tgatgaacca aaaacgaaat atcttgattg aacaagtgaa cctacattat
     7201 ttgcggctga tccaattgga aagcgaatta gacattcgtc cacgtgtatt ggaaatcggc
     7261 agacgttccg ctaaactgga tatcgaagtg tttattgaaa acaccatcgt ttccaaagca
     7321 atcgttgttt gtcaagtaat ggaacgttct tagaaagcag gagtgaaatg acagtacagc
     7381 aagaaatagt agatacaata aaaaaatacg ataaaatcat tatccacagg catttgcgcc
     7441 ctgatcctga tgcattagga tcacaagtag gattagccga actattacgg gaaagtttcc
     7501 cagaaaaaga aatacgtcaa gtaggtgaaa cgattgaagg gcttcgcttt ttagcagaaa
     7561 tgcaagaagt tgaagatgtc ttctaccaag gagcgctagt catcgtgact gatacagcta
     7621 attcacccag aatcagtgat caaagatatc agttggcaga aaaactgatc aaaatcgacc
     7681 atcatcccaa tgatgaccct tatggagatc ttgtgtgggt aaatacaaaa gcaagtagct
     7741 gtagtgagat gattgcagaa tttgctttga tgtttcctga atctttaaag atgaacgtta
     7801 aggctgcacg gttactttat gcaggaatcg tcggagatac agggcgcttt ttgtatccct
     7861 ccacgacgtc ccgtacatta gagattgctt cgatattgag aaattatcca tttgatgcaa
     7921 gtgctttgaa cagagagatt gaacaaatgc cgatgaaagt cgctaaactt tccggttatc
     7981 tttatcaaaa tatccaggtg gatgaaaatg gtgcaggaag aataatcctt ccgcaagaaa
     8041 ttcttgataa atatggaatc gatgattcag agacagcttc gatcgtttca cttcctggag
     8101 tgattgacga agtgctggct tgggggattt ttgttcagca gccttcaggg tattaccgcg
     8161 tacgtttacg ttctaagggg ccgatcatca atgaattggc taaacgccat catggaggag
     8221 gccatccatt agcaagtggt gcgaatgcaa aagatttagc agaagtagag gaaatctata
     8281 aagagatcca agatatatgc caatcgtata agagcagaaa aacagaatga tacttcgttc
     8341 gaaaacgtgc cttttgtagt aaaaatcaca aatgtatctt ttcaagatct tctttttccg
     8401 ttacaatagt gctcactaaa gattttaaag gagaaagaag atgacattac caaattgccc
     8461 agaatgtggc tcagagtaca catatgaaga tcgaggcttg ttgatttgcc cagaatgcgg
     8521 gaacgaatgg aacaaagaag aacaagaaac tgtcatagaa gacggattag tggtattaga
     8581 tgcaaatgga aaccgtctgt tagagggcga tagcgtgaca gtgattaaag acttgaaagt
     8641 aaaaggtgcg tcaggtgcca ttaaacaagg aacaaaagtc aagaacatcc gtttagtaga
     8701 aggcgatcat aatatcgatt gcaaggtaga aggatttgga ccgatgaaac tgaaatcgga
     8761 atttgtgaaa aagaattaaa gaatattcaa ggtcttttca cattcttttc ctactactaa
     8821 tgataaaata aagtaaacga aaaggagtga aacgaaaatg tatggttatg gaatgtatgg
     8881 ttttttagat ccgacatttc ttttggtcat tcttggatta gttatttccg gaattgcgtc
     8941 agcgtacgtc aatagtactt tccgaaaata cgatcaagtg agaagcaaaa acaatgtgac
     9001 tggtacacaa gcagcacaat atatattaca aagtcaacag ataaataatg tcggtgttca
     9061 acaaattgcc ggagatctca cagataacta caattcaggt aataagatgt tgagtttgtc
     9121 tcaagcaact gcacagtcta cttcagttgc tgcgataggt gtagcagctc atgaatgcgg
     9181 acatgcggta caagatgctt cgggttatgt cccattgaag attcgtgcag cacttgttcc
     9241 agcagctaac tttggttcga tgatttcgtt ccccttgatt atggtaggtg tattgcttag
     9301 ctggaactct acgttaatca atatcgggat tttcgctttt tctttagcat tacttttcca
     9361 attggtgaca ttaccagtcg aatttaacgc gtctcggcga gcaatccaaa ttttgagtga
     9421 aggcggacta ctgacggaag aagaagtacc gatggcaaga cacgtattat ttgcagcagc
     9481 actgacttat gttgcagcag cgctttcgac attcctgcag ctgttgcgct tgatcttatt
     9541 gttcggcggc aaccgaagag attaaaataa agaagttgtg aaatcagcgc tttgatctga
     9601 gctaataccg agagttagct gattgaatac catttatttg gaataagagg atgtgacaag
     9661 aattttgtca catcctcttt gttttgttct cataaccttt tttcatatac agttcatgaa
     9721 ccgcttttat taacttgcca aataacactg tattgtgcgt tgtatccaga tagaaagtct
     9781 ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat caaatcagca tgttctgaat
     9841 atgcggtaat tttaacagca tctttgcgaa ataactgctg caaatggctt tgaagatgat
     9901 aatttcctgt aaactgtttg gttatctgca aatggatgag taactgctct ttcgtttctg
     9961 tttgtagtaa gtgttggatc aattgactaa ggtattgact gatttggttt ctttccccat
    10021 tttttgaaaa aattgtatcg attttttctc ttaatttctt ttcctgtata aaagaactag
    10081 aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa actactgctg gattcaccca
    10141 acagttcgaa aaaaacaact gccaacacaa gaaaataaaa ataaattggt attttatttt
    10201 ctaaatggtt gagaggaaac tgttgagtta agtaagtcat caattgaccg acttgtcgac
    10261 aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc ctgtttttgt cgaaaagaga
    10321 tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta ggctctctct gtttctgtct
    10381 cttcacaagg gcgactcact gaaaaagaag taaagtattc tgaataatca tgtgtatgct
    10441 gcaataagtc catcaattca gtgatttcag aagaagtttt tggaaaatat gcctgttgtt
    10501 tgtttcgtaa tatgaataaa gttaacagca agtagatatt tttttcttgt tttgaaattt
    10561 tttctatttg ctcatctgta ctgttcattt ttagtgcatt taattcaagt gaggaaaatg
    10621 gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta aagatagaga cggatagata
    10681 actcatttcc ttcaagagta cagcaatttt ttacagatag gatccgtaaa tggaattttt
    10741 ttaaggaatg attgagctgt tttgtcagtt tttcgagata ggagggcgat atataaagtt
    10801 gttcaagtaa atattttttt ggtacagcag tttgtggata attgattagt aacatcaaat
    10861 agttaaaacg attggattct tttaggtagc ataattttaa cttagaaaaa acagctaact
    10921 ttttagtttg atcggtttct ttggtttgga tctctccttt atgattagaa taaataaatt
    10981 catcaatcaa taaaaattgc ttgagtgata catttatttt tcggatcagt tctttgattg
    11041 tccgttttgg aatatgtaag tcattggata attcttgtaa agaacaatta tttttttgaa
    11101 tgaccatttg tagaacaagt aactcttttt ttcttgcttc aattagcata aatacctcct
    11161 ttttattctg tttttgtata tagttagaca aaaactgtga gt
//
LOCUS       DAXLPI010000081        11148 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXLPI010000081 DAXLPI010000000
VERSION     DAXLPI010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.081"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            6..305
                     /locus_tag="ACF3T5_001915"
     CDS             6..305
                     /locus_tag="ACF3T5_001915"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3632515.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACF3T5_001916"
     CDS             378..1739
                     /locus_tag="ACF3T5_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFD3632516.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACF3T5_001917"
     CDS             1761..2021
                     /locus_tag="ACF3T5_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632517.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACF3T5_001918"
     CDS             2035..2787
                     /locus_tag="ACF3T5_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFD3632518.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACF3T5_001919"
     CDS             complement(2881..3486)
                     /locus_tag="ACF3T5_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD3632519.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="ACF3T5_001920"
     CDS             complement(3542..4516)
                     /locus_tag="ACF3T5_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFD3632520.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="ACF3T5_001921"
     CDS             complement(4894..5613)
                     /locus_tag="ACF3T5_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3632521.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACF3T5_001922"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="ACF3T5_001922"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFD3632522.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="ACF3T5_001923"
     CDS             7164..8561
                     /locus_tag="ACF3T5_001923"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFD3632523.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="ACF3T5_001924"
     CDS             8642..9082
                     /locus_tag="ACF3T5_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFD3632524.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="ACF3T5_001925"
     CDS             9229..9555
                     /locus_tag="ACF3T5_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFD3632525.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="ACF3T5_001926"
     CDS             9556..10305
                     /locus_tag="ACF3T5_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFD3632526.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="ACF3T5_001927"
     CDS             complement(10331..10741)
                     /locus_tag="ACF3T5_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFD3632527.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3536 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaatt
//
LOCUS       DAXLPI010000082        11010 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXLPI010000082 DAXLPI010000000
VERSION     DAXLPI010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.082"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            128..628
                     /locus_tag="ACF3T5_001928"
     CDS             128..628
                     /locus_tag="ACF3T5_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3632528.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(818..1162)
                     /locus_tag="ACF3T5_001929"
     CDS             complement(818..1162)
                     /locus_tag="ACF3T5_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFD3632529.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1316..2236)
                     /locus_tag="ACF3T5_001930"
     CDS             complement(1316..2236)
                     /locus_tag="ACF3T5_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFD3632530.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            2423..2914
                     /locus_tag="ACF3T5_001931"
     CDS             2423..2914
                     /locus_tag="ACF3T5_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFD3632531.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(2938..5313)
                     /locus_tag="ACF3T5_001932"
     CDS             complement(2938..5313)
                     /locus_tag="ACF3T5_001932"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFD3632532.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            5639..6265
                     /locus_tag="ACF3T5_001933"
     CDS             5639..6265
                     /locus_tag="ACF3T5_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD3632533.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6288..6992
                     /locus_tag="ACF3T5_001934"
     CDS             6288..6992
                     /locus_tag="ACF3T5_001934"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFD3632534.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7184..8017
                     /locus_tag="ACF3T5_001935"
     CDS             7184..8017
                     /locus_tag="ACF3T5_001935"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFD3632535.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            8214..10142
                     /locus_tag="ACF3T5_001936"
     CDS             8214..10142
                     /locus_tag="ACF3T5_001936"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFD3632536.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            10280..10735
                     /locus_tag="ACF3T5_001937"
     CDS             10280..10735
                     /locus_tag="ACF3T5_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFD3632537.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            10815..10943
                     /locus_tag="ACF3T5_001938"
     CDS             10815..10943
                     /locus_tag="ACF3T5_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632538.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
BASE COUNT         3372 a         2058 c         2028 g         3552 t
ORIGIN      
        1 catatttgta ttgttcgatg attcttaata cgatatgaca caataaataa aaataggcat
       61 ggctatttac aacaatagtg ctatagtaga aaacaaatga ataaggttac ttaaagaggt
      121 gaatacgatg aaactgatca aactatggga agcagattta aagaaagctt atcagctaca
      181 aactagcttt ggaccaaatg agagtggatt tatcaacaat gcttatggct atacatttgc
      241 tgaattttta gattatgtaa aaaaatgcga aaaaacgtct ttaggaatcg atcttcctga
      301 gagttacgtt cccgcaaccg tctttatttt agaaagtgat gaacatgagt atgtaggaat
      361 ttttaatttg cgccataaac tgaacgaaac attaagaaac ggcgctggcc atattggtta
      421 cggcatctcc cctaattatc gtaaaaaagg atatgccaca caaggattag cattagtact
      481 acaagaggca aaaaaaatcg gcattgatga ggcacttttg tctgtaaata aaaaaaatct
      541 tggcagtctt aaagcacaac aaaaaaatgg ggctgcgatt tatcgtgaag atgagaaaga
      601 atactatacg aaaatcaaac tgatgtaaaa aagaaggatt ccaaaaggaa agggatttga
      661 atgtccttta tcaatagaac acatttattt aattaactta taatatagca gacaaaattt
      721 tataaacaaa gaaacctttt atctcttttc ttctagaaag gataaaaggc ttttttgttt
      781 ttcctatttg cttctcatag ctagtcagcc aagatttcta tcgtatcccc aacgcaatcc
      841 gtgcataacg gctcatctta tctgtcgtcc atgctggata ccagactagt ttgacttctg
      901 ctttcgttac ttctggcact tcttttaatg cgtcatggat agattctgtt aaaatatctg
      961 ctaatggaca gcccatagtt gttagagtca tcttaatgac tgtttctcca ttttcaggat
     1021 tgaattctat atcataaatc aagcctaaat tgacgatgtc gattcctaat tcaggatcaa
     1081 tcaccatttc caaggcagca agaatacgtt ctttgattag ttctatttct tgtccagacc
     1141 gttcttggtt tgcttcgctc atcgtacgac actccttaac acgattttta tttatcatac
     1201 tcttttttta gctgcttgta aatgagaatt tagaagtaag attctcgttt aaatttataa
     1261 aaatgttctt attagatgta atagattaaa tttattctaa aataaacctg attcctcatt
     1321 ttttttgtga acgatttttt atttctggca atacgccagt tgttcgatac attttttgtt
     1381 cgttttcatt tgcaaaccgg ccaaatactg aatgagttgg taaaatattg ataaatgcat
     1441 cgtcgtcgat ttcatgaacg atctgatcaa gatcatacat ttcgtagcgg gtgatgacca
     1501 tcatgatcat gttcccttgt ttttttgaat agccaccgac agacggcagt aatgtcattc
     1561 cacgtaccat ccgttttgct agttcttctg ttacctcttc tggcttgacg gtaacgatca
     1621 ttgctgttac tttttgatga ctcgtgtgga tggcatcaat tacttgagtc attgcataaa
     1681 tggaaataat cgtatacaag gcactttccc agttaaagaa gaagcctgcg atgactacaa
     1741 tgataccatt tagaatcagc atgtagtttc cgactgtttt tccagttgtt tgtgaaagta
     1801 caagagagat gatatccatc ccaccagttg taaagcccat ttttaaagat aatcctacgc
     1861 caagacctaa taaaacaccg cctactaggg cattcatcaa aatattatca gtgatctgtc
     1921 cgactggaag cagcatcgtc atcaccgata cacatacaac attcacaaaa ctaaggatcg
     1981 ttgctcgttt tcctactttg agaaacccta atacaaacac agggatattc aaaagaaaaa
     2041 taaatgttcc tgtgtctatc ttgatatgaa accattcata taaaagcgag gaaatgattt
     2101 gagcgatccc attcatccct gcagaaaaga cattggctgg aatcaaaaaa taatttaaag
     2161 cgaccgcagc ggtcaatccg gttatcaaaa taaccactat tttctttaat ctttcatttt
     2221 tatgatacgc agtcataatt ttactcacaa gcacctctcc cctactttgg tattccaaat
     2281 gtaagaataa cagtttatct attaaaataa aatacttcga actcaaatga gaagaatttt
     2341 agcaaacagg taagaatctg ccctaatcct atgataagat agatgtatgg taaatattta
     2401 tatgtatatc aggagggttc ctatggcaaa agatgctagc ttcgacattg tatcagaagt
     2461 caatacagaa gaagtaaaaa atgcgattca gcaaacaaca aaagaattgt ccaatcgctt
     2521 tgacttcaag gattctacag tagaaatcaa attagagaac agcaatctag ttgtattgag
     2581 cgatgatgat ttcaaaatcg aacagatcaa agatgtatta tttggtaagc tgatcaaaag
     2641 aaatgtgcca atcaaaaata tccatttttc cgaatcagaa cacgctctag gtggcaaagc
     2701 tcgacaaact gccgatttgg tcaacggaat cgatcgtgaa aacgcgaaga aaatcactac
     2761 agctatcaaa aatgaaaaac tgaaagtaaa agcacaaatc caagaagatc agattagagt
     2821 gacaggtaag aaccgtgatg atctacaagc tgtgattgct ttattacgta aacttgactt
     2881 accaatcgag ttgcaattca ctaactatcg ttaatcaaaa aacgaggctg acgtctgtca
     2941 ttcctcgttt ttttgattta ctgcttcttt tgataaccaa aaacgctgta ttccttcttc
     3001 caattcccca acacttcgta cttctcttgc tgtttgccaa tgttgtggga ataaacgtcg
     3061 ataagaaaaa ctagaaaatc gttgatctgc caatacaacg attccttgat ccgccatgcc
     3121 acgaatcact cgaccaccag cttgcaagac tttgttcatt ccaggaagct gataagcgta
     3181 agcaaatcca aggtgttcct tctcatcata atatgatttg atcagttcct gctcatgatt
     3241 catctgcggc aaacctacac ctacgatcat actgccgatc aatcggtccc ctctcaaatc
     3301 gattccttct gaaaaaatcc cgccaagtac gcaaaatcca atcaatgttt cagcgggatt
     3361 tgtttgaaat tttgttaaaa atcgttcgcg ttcttcctca ttcatgtccg tttcttggat
     3421 tagcacattg ctgtcgggat attgctgtct gaatgcttcc accgcaagat ctaaatattg
     3481 ataagaagga aagaaaacga aataattccc gacctttcca tcagcaaaag catgaatcgt
     3541 ctctactaat tttgccagac tgtgttccct ttttcgataa gtcgtctcta ggtaatttgc
     3601 gatgaaaact tgctggtttc cttctgggaa aggactcggc agtttgtaag ccagactttt
     3661 ttgtcccccc agtgtttcct gataataaga gagtggtgaa aaactagcag aaaaaagaat
     3721 actgcttttt cctttatcca acgactgctc aagaaacaag cttggatcaa tacaaaactc
     3781 ttttactatc agatcgcgat acgttttttc gactgttgtt tcgtagtgat catcataaaa
     3841 ttcactgacc ttcaaaaaat gaagcaaatc aaaataataa ggaagcaatt gttcttgtga
     3901 tgggtgctca ggaaattctg ccagccattc tttaattttt tcgcttaatt gataacctgc
     3961 ctttactaaa gattcagccg gtgctttttg atgatgataa tcccaatgat cttcttttgc
     4021 tatttctcgg atcgaatcaa attcttttaa tagtttgttg aaacggcgat gaagtttttt
     4081 gaattccttt ggtatagctt ccttacttcg tttcgttttt tcataagata gttctgccga
     4141 atacatctct cgggaacgat tcaccaaatt atgtgcttcg tcaatcaaaa acagatagtc
     4201 ctcgtttttt tcatcttcaa aaaaacgtct taaataaaca gttggatcaa ataaataatt
     4261 gtaatcccca atgattacgt cacaccagat actgacatct aatgatagtt cgaaaggaca
     4321 aagtgtatgc tttcttgcat aagtttcgat gacttcacga gtgatctgat tctcatgatt
     4381 caaaagatcc cataaacctt cattaatccg attgtaataa ccattggcat atgggcactg
     4441 atctggatta caggtcgtct catctaaaaa gcagatcttg tctttcgccg tcaaagtgac
     4501 actttttact tcagctcctg tatcttttaa tgctgtcata gcatcttcag ctacttgtct
     4561 agttattgtt ttagccgtta gataaaagat cttttctccc tcttcttccc ctacagcttt
     4621 caaagcgggg aataacgtag agattgtttt tcctgttccg gtaggtgctt ctacaaacaa
     4681 tttctgtttc gtacggatcg ttttataagc agctaccgct agttcacgct gacctttcct
     4741 atactcatca tgggggaatt tcaaagccat caaagaggca ttgcgtacag tgcgccagtt
     4801 ttcttgaaaa acaagccatt ctttataatc ttcagtcagc tttttaaaaa aagtttctaa
     4861 ctgctctctt gtctgatgtt caacctttct cgtgatcact tcttctgccg tctggaagta
     4921 agtcaattgc agatttattt cttgtaaatc atgttcttga ctatagatat aggcatacac
     4981 ccttgcttga tgaaagaaaa gttccacttg ttctggttct aaatcttcaa atcgtggttc
     5041 ggaggttttg atttcatcta tataataaat accgtctctt ttgaaaatac cgtctgcacg
     5101 gccttcaacg atcaggtaat attcatctaa ttgaactgtt gtttttaaaa agacttcttt
     5161 ttcatagttt tctcccgcat ctttttggag cttgcggtgg attctcgctc cttctaatgc
     5221 agtatggtta ctttttttac gattgtcgat acttccacgt cgcaaaataa attctactaa
     5281 gtgccgcaca gatatttttg actgtttgat catttccacc acctctttct ctctaagatt
     5341 acctgaaaaa acctagttta gcaaaaagag ctctctttcc aaacataaaa aaacaccctg
     5401 ctgattaggc agggatatag gatatagaat ttggattttt ggatgaatct aatgtaacaa
     5461 ggacatatta aaatgctatg aaaacttact ttccttctct ttagaatatc caaatacttt
     5521 tcatatagtc tgataattca gataagtaaa gagaaataag tacctgtttt tgtttcctag
     5581 caatgaatgc gttatactaa tggatgaaag aagttttggc tatcaaggag gaatgcacat
     5641 gcatatcgaa agattaaaaa caggaatgat tgaagaaaac tgttatttgg tctacaacga
     5701 agaagcactt ttgatcattg accctggaga agatgcggaa cgaatcaaac aagaaatcaa
     5761 aaaaacaaac caacagccgg tagcgatttt gcttacccat actcactacg atcatatcgg
     5821 tgcagtggaa cctttacgcg cgtattatca aatcccagtt tatgtcagcc cactagagca
     5881 aaaatggcta ggtgatccga ttttgaatct ttctggactc ggtagacatg atgatattgc
     5941 tgatgttatt gtctcgcctg cagaatatga atttgaaatg aaaacttata ctttaggaaa
     6001 catgtctttt cgcgtcgtcc ctaccccagg gcattctata ggaagcgtca gctttatttt
     6061 tgatgatttt gtcgtttctg gcgacgccct gtttagagga agtatcggta gaaccgatct
     6121 gtatacaggg aatttagaac aattacttca tagtattcgg acccagctgt ttgtattacc
     6181 tgatgaattt gcagtctatc caggacatgg cgatgcaacg acgattgaac aggaaaaaag
     6241 gacaaatcct tttttcaatc gttaaaacga cttaggagga gaataaaatg acagaaacta
     6301 cactttatat cgtacgacac ggaaaaacga tgttcaatac aattgaacga gtacaaggct
     6361 ggtgtgatac acccctcaca aagcaaggac aagaaggcat tcattattta ggtcgcgggt
     6421 taaaaaatgt cgacttttca tttgcttatt ctagtgacag tggtcgtgca atcgaaactg
     6481 cccgtattat tctaggagaa cattctaagg gaaaagaaat tccttatttt attgaaaaaa
     6541 ggattagaga atggtgtttc gggtctcttg aaggtggata tgacatggaa atgtggggtg
     6601 tgatccctcg tgtattgaat tttaaaacat atgatgaaat gtttaccaca gaagtttctt
     6661 ttgaacaaat tgctaatgct gtatacaact tagataccgc tgggtgggcg gaaacttatg
     6721 aaacattgcg agaccgtgta tggacaggat ttgaagatat cgcccatcac tgcgaaaaaa
     6781 atggtggtgg aaatgtttta gtcgtatctc atggtttgac gatcgccttt ttgcttaatc
     6841 tgatcgatcc taaacagcct gttcgtgctg ggcttttaaa tggcagcgta acaaaggtca
     6901 cttatgcaaa cggtcgattt tctatccacg gcatcaatga tacgtcttat atcgataacg
     6961 ggaaaacaca tagtcgcgac tcgtttctat aatggggata ttttaatttt taccagagaa
     7021 tcataaaagg caaagtctga acgagggctt tgtcttcttt tttctcttcg tgtacttatc
     7081 tgctttattt ctcaagtttt tcttaatatt aaaaaacaga ttgggtttct caaaaaataa
     7141 cggtatacta taagatgtat cttaattggg aaggaaggaa tttatgtttt ttgcaaatat
     7201 tatcaaagca gtgattctcg gtattattga aggaataacg gaatggctgc ccatcagtag
     7261 tactggtcat ttgatcctag cagatgaatt catcaaactg gatgccagtt cacaatttat
     7321 gtcgatgttc aatgtcgtta tccaattagg ggcgatcttg gctgtcgtcg ttctttattt
     7381 tcacaagtta aacccatttt ctccaagtaa atcaacggta gaaaaaaaag atacgtggac
     7441 actttggttt aaagtgatcg ttgcttgcat accagccatc atcattggtt tgcctttgga
     7501 tgattggtta gaagcaaatt tccacaagtt cttaccagtt gccattgttt tgatcgttta
     7561 cggtattgcc tttattattg tcgaaaaacg caacaaaaac aaaacacctc gttggacttc
     7621 tctgaatgac tttacgtacc aagcagctct tcttgttggg ttattccaag tcttggcact
     7681 tgttcccgga acatcccgtt ctggcgcaac gatcctagga tctatcttga ttggtgcttc
     7741 gcgttttgtc gctacagagt tttcattttt cttagggatt cctgtcatgt ttggcgcaag
     7801 cggcttgaag atcgtcaaat atttagctga agggaattca ttcaaaatgg aagaaactgt
     7861 tgttcttctt gtaggaacat tcgtatcttt tgtggtttcc gtcttagcta tcaaattctt
     7921 actaagctat ttgaagaaaa acgactttac cgcttttggc tggtatcgta ttatccttgg
     7981 tatcattttg attggttatt ggtttattgc aatgtagaaa aaaccagaac acttctctta
     8041 tttgagaaat gttctggttt tttattttct ttaataataa tcattataaa taataaaaac
     8101 aacatcaagc ttaatcacca aatgataacc attatcgttc tcatttagct gttattatta
     8161 tcaattacat ttttattatg ctatccttgt tttgacaagg aggtctaata acgatgacta
     8221 atcagaaaaa agcttttgca gcaacaatcg gctgcctttt atttatgatc ttcggtttta
     8281 tcttcgaccg attctctctt accatcaccc cactttttta tatcagtgcc attatcatcg
     8341 gtggatataa acaaacatcg gaaggcttga aagaattgtg gaatgatcgg acactgaatg
     8401 tggatttatt gatggcttta gctgctattg gtgcctgttt gatcggtaat tactttgaag
     8461 gagcaatgtt gacattcatc ttttgtttaa gcggtgcatt agaggaatac acaactaata
     8521 aaagtcaaaa agaaattact gctttgatga atcttcaacc acaaaaagcc caattattaa
     8581 aagaaaatgg acaaatgcaa gaagtagatg tttctcaact aaaggtggat aacttggtat
     8641 ttgtcgctaa aggagcagct gttccgatag atggcttttt agaaagcaca caggcaacga
     8701 tcgatgagtc tgcattaagc ggtgaatctg tgcctgtaga aaaatattta ggcgatgaga
     8761 tgtatgcagg aacgctgaat caaggaaatc ccatcacgat tcgagtaaca aaaacgagtt
     8821 ccgaaactgt ctttgctaag attatccaat tagtagaaga agcgcagaat acgccaactc
     8881 aaacagcttc atttatcgaa cgaatcgaaa ataattatgt aaaattgatt ttgcttgctg
     8941 ttcctctaat gatccttctc ccccactttt tcctaggttg gagttgggat gaaagctttt
     9001 atcgaggaat ggtattatta gttgtggctt ctccttgtgc gcttgtcgca tctgctacac
     9061 cagcttctct agctgctctt tcaaattctg caaaaaatgg gattttaatc aaaggcggta
     9121 tccatttaga aaagctttct gaattaaaag cgattgcctt tgataaaaca ggaacgctca
     9181 ctaaaggaaa accagttgta actgacagct ttttctttga tgaaaaggat ttggcacaac
     9241 aacttcttgt tgctatggaa caaaaaacga cgcatccact tgctcatgcg attttgcaat
     9301 atttcgattg cacaatacct gaagaaatcc aatacttacc aatagaagaa attactggtt
     9361 ttggattaca aactacctat ttaggtgcgc aatggaaagt tggcaagcac gcatatgatc
     9421 ctgagacgat gataatcagc aaagagattg cggaaatgat cgaacgtttg gaaaatcaag
     9481 gcaaaaccgt gatttatctt tctaaagatc agcagttaat tgctgtttta ggtctgctag
     9541 atattccaaa agcaaatacc caacaagtaa tttcctactt taaatcccaa aacatccata
     9601 caagtatgat aaccggcgac cattctggca cagcaaaggc cattgctgaa caagtaggga
     9661 ttgaacatta ctatgcaagc tgtactccgg aagaaaaaac acaactagtc aaaaaagaga
     9721 atgaacttta ccaagtaaat gcgatggttg gtgatggagt caatgatgcg cctgctttag
     9781 cagccgcttc tttaggtgtc gctatggggc aagggacaga tgccgctatg gatatcgcag
     9841 acatcgtctt aatgaaaagc gacttaaata aatttgtcta cagtcatcgg ctatctttaa
     9901 aaatgcggcg gatcatcaaa caaaatatta ttttttcgat tcttgttatt ctcttgttga
     9961 ttgcttctaa cttcctgcaa tttttaaatc tacctcttgg agttgtagga cacgaaggaa
    10021 gcacaatatt ggttattctg aatggcttgc gtttactaag acctttacct gatctttctg
    10081 attcttcaaa aaaatgtaag tcttgtccac tatacaaagc gcggctgtct tctgtcaaat
    10141 aagcaaagtg tttttttatg attttgttta ttttatgaaa aaaacagctt atgaataggt
    10201 cttgaaggat gaattcctac ttttgtaact ttctaaaaac atgttatgat tgtttcgaag
    10261 aaagtgagga atatatttca tggaaagttg gcttttttta ggaattattt tacttgtagc
    10321 aattttagct aaaaatcaaa gtctgatgat agcggcagct tttgttttga ttatgaagct
    10381 cataccagcc tcccgttcct ttttcccttg gcttcaaggc aaaggaatca atattggggt
    10441 gacgatcatc acagcggcta ttctaatccc tatcgcaaca ggagaaatcg gtctacgtga
    10501 tctattagaa gcctttaaat cccctattgg ctgggtagct gttttttgcg gtgggctagt
    10561 ggctgtctta tcttccaagg gtgtaggatt gattgcttct gatccacaaa tcactgttgc
    10621 acttgttttc ggcacgattt taggtgttgt cttcttaaga ggaatcgccg ccggaccggt
    10681 cattgcttct ggtatcactt actgtatctt acaagtattt gctttaggaa aatagaacaa
    10741 attttgtcta ttttaggaga ttcttaacac ccccaattta ctgatccgtg atatactgac
    10801 aacgaggtga tacaatgttc gaaaaattaa agtatttcat aggaacaatc ggctggtttc
    10861 ttctagcggt acttttgatt ctgggcttag cttatctatt atttcctgat ttgatacaaa
    10921 atgtgtttga tactgctttt tgattgacct attaaaacga tataggggat gctcctgcga
    10981 tgcaggggct ttttttaatc aatgttttgt
//
LOCUS       DAXLPI010000083        10982 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXLPI010000083 DAXLPI010000000
VERSION     DAXLPI010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.083"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            87..2876
                     /locus_tag="ACF3T5_001939"
     CDS             87..2876
                     /locus_tag="ACF3T5_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFD3632539.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2923..3450
                     /locus_tag="ACF3T5_001940"
     CDS             2923..3450
                     /locus_tag="ACF3T5_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFD3632540.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3471..4661
                     /locus_tag="ACF3T5_001941"
     CDS             3471..4661
                     /locus_tag="ACF3T5_001941"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFD3632541.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4701..5999
                     /gene="asnS"
                     /locus_tag="ACF3T5_001942"
     CDS             4701..5999
                     /gene="asnS"
                     /locus_tag="ACF3T5_001942"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFD3632542.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            6929..7603
                     /locus_tag="ACF3T5_001943"
     CDS             6929..7603
                     /locus_tag="ACF3T5_001943"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFD3632543.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLIAQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            7600..7941
                     /locus_tag="ACF3T5_001944"
     CDS             7600..7941
                     /locus_tag="ACF3T5_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632544.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSFFAQF"
     gene            7971..8330
                     /locus_tag="ACF3T5_001945"
     CDS             7971..8330
                     /locus_tag="ACF3T5_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFD3632545.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGISLIVNIIWLIISGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            8591..8713
                     /locus_tag="ACF3T5_001946"
     CDS             8591..8713
                     /locus_tag="ACF3T5_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632546.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            8855..10936
                     /locus_tag="ACF3T5_001947"
     CDS             8855..10936
                     /locus_tag="ACF3T5_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFD3632547.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
BASE COUNT         3651 a         1936 c         2403 g         2992 t
ORIGIN      
        1 ttctttaaaa agcgatgaga gtatttttct gcactttgta gtataatttc aaaagaacat
       61 tagaggcagg agaagaggaa gatattttga aaccaagaac ttatgcagtc gttgatatcg
      121 aaacgactgg gacgaatcca aaagaagatc ggatcatcca atttggctgc gtcatgattg
      181 aatcaggtcg gatcacttca cgtttttcga tcgatattca tccagggcgg aagatttcaa
      241 aacaaatcca gcatttaaca gggatcacga atcaacgcgt ccaaaaagcg ccttatttcg
      301 aagatgtcgc ccaaacgatt tacaatcttt tggcagacac catatttgtc gcgcataata
      361 tttattttga ttataatttt ttgaaccatg aactcatgcg atgcggactt cctagtttga
      421 agataccagg catcgataca gtagaacttg cacaaatttt cttgccgacg gaaccaagct
      481 ttcgcttagc tgatttgtca gaaagccttg gatttattca tgagaatcct catcaggcag
      541 atagtgatgc cgaagtgaca ggtcaacttc ttttgctgat cgaagaacgg atgagaaaat
      601 tgccaatcat cacgttagag cagattgcgc gattaagtag acacacaggt atggatacga
      661 gtcgtttcat ttatcatgtg attgaagaaa tgaaagaaaa accagagcca cttgatccct
      721 cattagaaat cgtcgatggg ctagctttgc agaaaaaaga agtggaacta ttcacatccg
      781 tccattatgg tgagcggaca tatccaagaa aaaaacaagc aaaagaaaaa atgtttggta
      841 aaacactgat gtatcgaaaa gaacagaaca gactgatgaa tgcggtctac gaccatttca
      901 caaaagatga atcaaaagat ttgatgatcg aagcagctac aggaatgggt aaaacaatcg
      961 gttatctgct gccattgagt tatttggcta cacctgaaaa acccgctgtc atcagtaccg
     1021 tttcgctagt tttgcagcag cagatcatag aaaaagacat tccattgctg aatcaattgc
     1081 tagatcagcc catacaacct gtaattatca aaagctatcg ccactatatt gatttacagc
     1141 gttttaaagg cacacttgtg gaaccgccag aacaaaaaca atatgctttg tatcaaatgg
     1201 cggtcttagt ttggctgacg caaacgaaga ccggtgattt ggatgaatta catttaacaa
     1261 atttgaacca ttcattcttt gcagatatcg cgcacagagg tattggattt ttagcaagaa
     1321 atcagtcatt ttacgaacaa gactttgttc gtttcttgca aaagaaaatc aggcaaagta
     1381 attttttgat tgtcaatcat gcgtttttaa tacaagaaac acagcgaaaa gaaccattgc
     1441 ttccagctag tccatttttg ttgatcgatg aagtacatca tttgccggaa atcgctgaaa
     1501 aagtaaatga caaacgatta agtattaact atttccataa acaaatccag caattagaag
     1561 aaccaaatca gctgtttgac cgaatacaaa gctatttacc gaaagaacat gaagccttac
     1621 gctggttatc gatttatcga gaagaacttt ctgcgctatg cgaagcacag atttgtatgg
     1681 cagaaggatt aatgagttta gtgagcggca agcaacctaa cggacagtat ccagaagaag
     1741 tcatgatgac aaaagagatg ttggatcagc tgccactgga atctgggaca gccattgatc
     1801 acctgcagct tttttatcag gagatccagc agctacagtc acaaatgcgt ctgacttttg
     1861 agattatcca agaaagctgg acaaaccaga tgcggatcga tttagcaagt ttatatagct
     1921 tatttgatca attagacaag cagtacttgc tgatggatca atggctggaa gactggcgag
     1981 atcatttgat ccattggttc gttcctggga acacagcgca gcagatgatt tttcatctcc
     2041 atgattttca agcggctatg ttgccgtcga ctgtttggta tcctcgatat gatcgtattc
     2101 tttatattgg aggaacgctg aagatcggcc ctaatcgaca ctatctctct aagcggcttg
     2161 gcattgaagc agcacctctt aaagtgattg cctctcctta tgattatgag aaacaggcaa
     2221 ggatctatgt gccaaaagag gcacctgccg tccatcaagt cagcaatcaa gcatatatcg
     2281 attatttaga gacagcactt acaggtctga tcgaacaaga agatcggcca atacttgtgt
     2341 tattcacttc tcatgatgtt ttgcagaagg tctatcagaa aatgcatctg tctatgctag
     2401 aaaaaggacg agaaatacta gctcaagggt ttggcggaag ccgggagaag cttttgaaac
     2461 gcttcatttt gtcagaaaaa agcgtattgt ttggcgctga cagcttttgg gaaggtgtag
     2521 atttgcctgg agatgctttg cagatcgttg tagtcacacg tttacccttc gataatccgc
     2581 ttcgcccgat ggtcaaagct aggaacgctt atttagaatc agaggggatc aatccttttt
     2641 accaggaatc cgtacctaaa gcagctttga agttacgcca agcattaggt cgcttgataa
     2701 ggacggagac agatcgtggc gtattgctta ttttggatcg aagactagtc actgcaaaat
     2761 atggtgcacg gatcatccat gcattaccga aaaaagtacc gatcaaagag ctagctatgg
     2821 aggaaatcta tcaggacatc catgaattct taaagaaaaa tgaagaagga agttagagga
     2881 gctgtctttt ttcataatta aaatctgcta taatgaaaag gaatgaagat aaaggagcga
     2941 cgacccttga agaaaaaaca gacaaaggaa aaaacgatta atcgcgtgtt acttggcttg
     3001 atcgtatttt tattagcggt catcgtgatc agctcgattt tttacttgcg ttccagtcgt
     3061 ccggcggtgc aggcgaagaa agaagcagta gaaatcgcaa agaaatatgc aaatcttgag
     3121 gaagccgatc atttttattg gtttactcga aagaagacat acttttcact gacaggtaca
     3181 aatgataaag gaaaagaaat cgccgttatc attcctaaat ctggtgaaaa agtaaaagta
     3241 ttggatcaaa caaagggact gactgaaaac gaagcaaaac agacaattgc tgcggcccat
     3301 cctgaaatcc aaatcgaaaa agctgcttta gggatgtatg atgatcagcc tgtgtgggaa
     3361 gtagtaggaa aagaaacaaa tgggaaactg aattattacc tgattggctt tgaatctgga
     3421 gatgaagtca agacaatcaa gggaatttaa aaggtaaagg aatgaatggg atggagttat
     3481 caaaaagaac aaaacgatta gaaccgtctg tcactttggc agctgcggca aaggccaagg
     3541 cattaaaagc aaaaggcaga gatgtgctga gtttgactgt gggagaaccg gattttgcga
     3601 caccagaaaa catccaagaa gcagcaatcg aagcaatccg caatgggaaa gctagctatt
     3661 atacaccaac agcaggaatc ccagagcttc gacaggcaat cgttgattat ctgaagaaaa
     3721 actatggact tgtctatgaa ccttcacaaa cagtcgtaac agacggagcg aaatttgctt
     3781 tgtataccct tttccaaacg atccttgacc cgcaagatga agtcatcatt cctgtgcctt
     3841 actgggtcag ttacggtgag caagtaaaac tggcagaagg acttcctgtc tttgtgaaag
     3901 gggaagaatc caatagctgg aaagttactg ttgagcagct agaacaagca cgttcagaaa
     3961 aaacaaaagc cgtaatcatc aattctccat cgaatccgac tggaatgatt tatagcaaga
     4021 atgaactgca ggcaatcggt gaatgggcag taaaaaatga tattttgata gttgctgatg
     4081 atatttatgg tcgtttagtt tataatggca atgagtttac tccaattgca acgatttcag
     4141 aagcaatcaa aaatcagacc atcatcatca acggcgtgtc taaaacatat gcgatgacag
     4201 gctggcgcat tggttatgcg gtggggaatt ctgagatcat caatggaatg atcgctgttg
     4261 cttcccagtc aaccagtaat ccgacagcag tcagtcaata tgcagctgtt gaagcattga
     4321 aaggcgagca agatacagtg gaagaaatgc ggaacgcctt tgaagaacgc ctgaatacgt
     4381 tatatccgct tgtggcagaa ctgccgggtg tttctcttga aaagccgcaa ggagcttttt
     4441 atctttttcc taatgtaaaa gaaacgctac gcatgtgtaa atatgaaaat gtcacacaat
     4501 gggtagaaga tcttttagaa gaaacagggg tagcgcttgt gactggcgaa ggctttgggg
     4561 caccagaaaa tgtccgcatg agttatgcta ctgatcgcgc tacgttagaa gaagcggtac
     4621 gcagaatcaa gcaatttatt gagagtaaaa gtcaaaatta atcctgaaga aattcaagga
     4681 atataaaagg agagtcatac gtggaacaaa ttcaaatcat cgattcgaaa aaccatgtgg
     4741 gagaaacagt aacaatcggc gcttgggtcg caaataagcg atcaagcgga aaaatcgcat
     4801 ttttacaatt aagagacgga acagcctttt tccaaggtgt agttgtaaaa agcgaagtgc
     4861 cggaagaagt tttccaaaca gctaaaaatc tacaccaaga aacatccgtg ttagtaactg
     4921 gagaaatccg tgaagacagt cgttcaaaat ttggttatga aatcggaatt acaaatgtgg
     4981 aagtggtaag cgagagccac gattatccga tcacaccaaa agaacacgga acagacttct
     5041 tgatggatca tcgtcattta tggctgcgtt cttcaagaca acatgcgatt atgcagatca
     5101 gaaatgaaat cattcgtgca acgtatgaat tctttaatca acatcatttt gtcaaaatcg
     5161 atccgccgat cttgactggt tctgcaccag aaggcactac ggatctattc gaaacaaatt
     5221 actttgatca aaaagcttat ttatctcaaa gtggccaatt atacatggaa gctgctgcta
     5281 tggctttcgg aaaagtcttt tcattcggtc cgactttccg tgctgaaaaa tcaaaaacac
     5341 gacgtcattt gatcgaattc tggatgatcg aaccagaaat ggctttcact catcaagaag
     5401 aaagtttaga agtacaggaa caatatgtag cattcttagt acaaaatgtt ttagatcatt
     5461 gcgaatacgc attagatgta ctaggacgag acaaagaagt cttaaaaact tatacaaaat
     5521 tgccattccc tcgtattact tacgatgatg caatcgaatt actgaagaaa aatggatttg
     5581 atgacatcga atggggcgaa gactttggtt cacctcatga aacgttcatt gcgaattcat
     5641 tcgaacagcc tgtctttatc ctaaactatc caaaagccat caagccattc tacatgaaac
     5701 cacatccaac aagagaagac gtggtgatct gtgcagatat gatcgcacca gaaggttacg
     5761 gtgaaattat tggcggaagc gaacgggcaa cagactatga tttcttgcta gaacaaatcc
     5821 gtaatcacgg tttggatgaa aaagaatatt catggtatct tgatctacgt aaatacggtt
     5881 ctgttccgca ctctggtttt ggtctaggcc ttgaacgaac agtcacttgg attgctggta
     5941 tcgaccatgt gcgtgaagct attccattcc cacgtttgtt gaaccgaatt tatccataag
     6001 aggttgtgaa atcagtgcct tgacctgagt attagagaaa caaaacggaa aaatagcttc
     6061 tcatatttta ccgttttttt agctaatatc gagggtcagc tgattgaaca ccgtttatat
     6121 caagaggttg tgcgcctgcc gttcagctcc gagtcacaag gagtaatttc aaaaaacagt
     6181 ttttcatgtt tgatgactat tgtgacttgt gaccgaggag ttggccggag agcaccgttt
     6241 attctaaaaa agaactgcga actctcgcag ttcttttttt taaatacggg ataaaggaag
     6301 tttccagcag acaagagaag aaagcaaaag aaattgctat ttgattcaca aggttctata
     6361 acgaaatcgc aatcaatcaa acatttacaa agaaggtaat tcaaatggaa atctaatttg
     6421 atgatgcttc tgctgttgaa ataaagcatc atctagcacc tgaaaacaaa ctaatagtaa
     6481 cattcgaaga tggagtaggt ccgtattttc agcatgcaat aatccatatg taagttcaat
     6541 tcacccttga aatcatcgga ccagataaag ttatgaccaa tctatcccaa gcaatctaga
     6601 agagatatta gtaaagggac atttactaga agtcttagat gagcctataa atatatgttt
     6661 tgatggcgtc atcagtggtc tgattgacgg aagcttgggt atgatcgatc tctctggacc
     6721 aaacaacgaa ggaatcaagg ggaatccggc acgttaagaa aaaaatagca taaaaaacta
     6781 gacacttgat gagcgttcat tcaattgttt gaagggacta ggctgatgtc attttttata
     6841 catatatttt ctctttcgtc ttgatagggc ttatgggata atataaggaa gaaaatcatc
     6901 gttttattca aaaaggagtt tttgacaaat gaaaaaacgt atattattta tcggtgatag
     6961 cattacagac gcaggaagag accgagcaga agcaactgat ctaggtaaag gttatccact
     7021 tttgattgcc ggaaaactaa tcgcccaata tccaacgatg gaatttgaat gcatgaaccg
     7081 tgggatagga ggagacaaaa ttgcagatct gcaagtacgc tggcaagaag attgtttagc
     7141 tttacaacct gactatgtat cgattttgat cggaatcaat gatacctggc ataatactag
     7201 cgatcaggct atattcggaa ctgagaaatc tgctcagcaa tttgaatccg tttatcgaaa
     7261 attactaaat gaacttcaaa cagcggaaat ctgcaacatc atcttgatgg agccgtttgt
     7321 acttcctata accgacgacc gtcatggctg gcgtaaagac ttagacccta aaatccaaat
     7381 tgttcgtaaa cttgctaagg aatatggcgc attgctggtt cctttagatg gtatattcaa
     7441 tgctttaggg attgcttatg gtttttcgaa atatacaaga gaagatgggg tccacccaac
     7501 gattggtgga catgagatca ttgcacaaag ctggatagat gcaattgccg cagtcgattt
     7561 ttttagtgga gataaaaatg agcgaaagaa aagtgaaaaa tgatggaaat aaggtgggca
     7621 gtaaaagaag atttacctca aatcaatcag ctgattactg atcatccaga atcattcgta
     7681 aaagcaaaaa cggcagctga cctaaatgat aagaataaac tattgctcgt tgctgtaaca
     7741 aaagaaaaac ggattatcgg atttgcgcaa gcggacgtgc aacagtcaga agaacgattg
     7801 gatcagcgac catcaggaaa actaatccaa cacacattag taacaaaaga aaatacggaa
     7861 gaaatccaaa aaaaatttgc agaatatttg atgctggaag ctaaaaaaag aagaataaaa
     7921 tcattttttg cccagtttta atcaaaaaca gtaatagaag gagaaatata atgagttgtt
     7981 taggaaatgt catctggttt atattcggcg gactaatcgg tggtcttagc tggttagcag
     8041 cggggatact ttggtgtatc acgatcatag gtatcccaat cggtatgcaa tgttttaaga
     8101 tcgcctccct ttcttttgcg ccttttggca aagaagtaat ttatcaccag tctggcatat
     8161 cattgatcgt taatatcatc tggctgatca tttcaggcct accaattgct ctcgcacatg
     8221 tcgtcagcgg catccttcta tgtatcacga tcatagggat accttttgcc aagcaatctt
     8281 tcaaacttgc tcgattggcg ttgatgccat tcggtgcaga aattgtataa aaagagaata
     8341 ataaaggtta gaaaacgcat agagtcaggt gggatacatg tatttcacct gactctatgt
     8401 gttttcctgt ataagttctc ccatgtgact tttacgtctt ttatttatat gggattactg
     8461 tttatggaaa gttgaaataa ttttcattaa agaactcttg ctatattttt gaaagcttag
     8521 tggagcttcg aatatctggg aaggacattt tgcagggatt gaaaaaagat attgtcctat
     8581 cgaaaaatta atgaatttag acaaattgcc agcaacaggc ttcaaactta gttgctatcc
     8641 tgtcaagatc aagaaggcaa gtgcgggttg gataagagca gtggcaatga tcgaaaagaa
     8701 aaagaaagaa taagccaaaa catctgtatg gaagaaattt tccgatgcag atgtttttca
     8761 ttttttcttt cggatacctt gacatatgaa taaccgttca tttataatta gttatgaaga
     8821 gatattcata tgttgaggcg aggaggtcat taaaatgaaa agttacagat tagaaggttt
     8881 agattgtgcg aactgtgcaa tgaaaatcga aaaaggagtt cagaagatca atggagtcaa
     8941 ggaagcaacg gtaaacttca cttctggaaa actaacgatt gacgcagagg aagatcattt
     9001 ggctacgatt gaacaagaaa taaaaaaagt agtgaaggaa ttagaaccag atgtcaaagt
     9061 aacagaaata gataaggaaa aagtatctga acatggaaat gaaaaagaga gaaatacgct
     9121 gttccggatt ttattttccc ttgcaggtat cgctttattg ctattatttg attttaatga
     9181 accaattcgc ttgatagggt atttattgat ttatctgctg ataggatatg atgtagtgaa
     9241 gaaggcagtg atgaatatcg tcaaaggaaa aatctttgat gagaattttc ttatgtctgt
     9301 tgctacgatt ggcgctatgt tgatcggtga atatccagaa gccgtagcag tgatgctatt
     9361 ttatcaaatc ggcgagtatt tccaaggatt ggcagtcagt cattcaagaa aatcgattcg
     9421 cgaattgatg gcgatccgtc cagaaacagc tcacgtgcag acgacggaag gattgatgac
     9481 cgtgaaccct gaagacgtgc agatcggtca gtttgtactt gtgaagcctg gtgaacgtgt
     9541 accgttagat ggaacgatca tcgaaggaga atcattagtc gatacgtcag cacttacagg
     9601 agaatcagtc cctaaatctg tatatgttgg tgaaacagta ttaagtggat ttatcaataa
     9661 aaataaacca tttttagttc aagtagaaaa atcttatgaa aattctacta tcagcaaatt
     9721 attagaatta gtagaaaatg ccagcagtaa aaaagcacca gctgaaaatt tcattactaa
     9781 attcgcgcgc tattacacac cagtcgttgt aggattggcc gttttattag ctgttttacc
     9841 tccacttgtt gtttctggag cggcgttttc tgaatggatc tatcgagcat tgacattttt
     9901 agtgatctca tgtccatgtg cattagtgat ctccgtaccc cttagtttct ttggcggtat
     9961 cggaggagca agtaaaatcg gtgtattagt aaaaggttca aattacttgg agttgcttgc
    10021 acaaactgag acagtggtat tcgataaaac cggcacatta acgaaaggtg acttttcgat
    10081 ccagacgatc gatacaaaaa tcgatccaaa gatattcatg caatatgttg cgagtgcaga
    10141 acagttttct acacatccga ttgctcaatc tgttttagaa ggctacgcag gtccgctttt
    10201 accaacagct aatatccaag aatttgcagg tgaagggatc ttagctgaag ttgatggcaa
    10261 acaagtatta gcaggtaatc acaaattgat ggaaagattt gaaatctctt ttccttcaag
    10321 tcaagaaatc ggtacattgc tttatctagc gatcgatcaa agttacagtg gttatcttgt
    10381 tattgcagat accttgaaag aagatgccgt agacgcattg gttcaattaa aaaaggctgg
    10441 tgtgaagaac acagtcatgt tgacgggcga ttcgaaaaaa attgctgatc atatcgggaa
    10501 gcaagtaggc gtggacaaga tatatagcga attgcttcca gaagataaag tgcagcggtt
    10561 agaggaaatc ttacaaagaa acaataaaaa gaccgcattt gtcggagatg gaatcaacga
    10621 tgcaccggtg ctagcaagag cagatgtagg aatcgcaatg ggtggattgg gaagcgatgc
    10681 tgcaattgaa gcagcagatg tagtcatcat gaatgatcaa ccatcgaaga ttgcagaagc
    10741 aatccattta gcaaagaaaa cattgaaaat cgtcaaacag aacattgtct ttgctattgg
    10801 gataaaaatt ttggtacttg ccttaggtgc ttttggtttt gcatcaatgg gagatgcagt
    10861 atttgcagat gttggggtga cagtattagc tgttttgaat gccatgcgaa gcttgcatgt
    10921 aaaaaataaa cgttgatcaa tgaagaggac gaatgaacgt cctctttttt ttatatcctt
    10981 tt
//
LOCUS       DAXLPI010000084        10878 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXLPI010000084 DAXLPI010000000
VERSION     DAXLPI010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.084"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..1001
                     /locus_tag="ACF3T5_001948"
     CDS             <1..1001
                     /locus_tag="ACF3T5_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632548.1"
                     /translation="VRYVNWEGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFI
                     IRGIEIAIGRKAFPLGLKEEDKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSL
                     FNSRNYTNENMELLRKDFEVVYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFL
                     QFVRTLWADGQVFLPERIYLLSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFV
                     KSLSLINSEQAGRQEIEIFVVSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELP
                     DECLCAKKRVVLCDAASYQNGLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1222..2985
                     /locus_tag="ACF3T5_001949"
     CDS             1222..2985
                     /locus_tag="ACF3T5_001949"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFD3632549.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3069..3527
                     /locus_tag="ACF3T5_001950"
     CDS             3069..3527
                     /locus_tag="ACF3T5_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFD3632550.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3636..4319
                     /locus_tag="ACF3T5_001951"
     CDS             3636..4319
                     /locus_tag="ACF3T5_001951"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFD3632551.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4466..5080
                     /locus_tag="ACF3T5_001952"
     CDS             4466..5080
                     /locus_tag="ACF3T5_001952"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFD3632552.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5096..5302
                     /locus_tag="ACF3T5_001953"
     CDS             5096..5302
                     /locus_tag="ACF3T5_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632553.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     regulatory      5527..5693
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            5841..6398
                     /locus_tag="ACF3T5_001954"
                     /pseudo
     CDS             5841..6398
                     /locus_tag="ACF3T5_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            6456..8495
                     /locus_tag="ACF3T5_001955"
     CDS             6456..8495
                     /locus_tag="ACF3T5_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFD3632554.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIPDKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(8604..9755)
                     /locus_tag="ACF3T5_001956"
     CDS             complement(8604..9755)
                     /locus_tag="ACF3T5_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFD3632555.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(9909..10439)
                     /locus_tag="ACF3T5_001957"
     CDS             complement(9909..10439)
                     /locus_tag="ACF3T5_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFD3632556.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(10538..>10878)
                     /locus_tag="ACF3T5_001958"
     CDS             complement(10538..>10878)
                     /locus_tag="ACF3T5_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFD3632557.1"
                     /translation="RIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQTELE
                     YQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMIDELAK
                     GKKMEKILRS"
BASE COUNT         3910 a         1857 c         2137 g         2974 t
ORIGIN      
        1 tagtacgtta tgtcaattgg gaaggctatg aaaataaaaa tccgtatgta gaagaagctt
       61 gtaacgagtt gattgattat gtagagactc atttttttaa aagaagatat tcggaagatg
      121 aaaagtcctt tatcatacga ggaatagaga tcgcaatcgg aagaaaggca tttccccttg
      181 gactgaaaga agaagataag aaaagcgcag aaacgaagcc gttatttcaa ttagtctgtc
      241 aaggattgga agagcaaaaa ggaattctag acttacaaga agaagatgtc tattatattt
      301 tttctttgtt caacagtaga aattatacca atgagaacat ggagctgtta agaaaagatt
      361 tcgaagttgt ttataaaaat tttgttctca atgacctatc actgaatgaa ttgattttac
      421 agattattag tatcgctgaa atcgacgatg cagaaaattt tattttcaag caatcctttt
      481 tacaatttgt ccgtacgttg tgggcagatg gacaagtatt tttaccggaa aggatctatc
      541 ttctatcaga aaaagaacaa cttctgtacg gaaaaattgt agaaatattg gaagagtgga
      601 aaaagaaaaa tcaaatccaa ctaagatgga atgaaaattt cattcgaaaa ttcgtcaaaa
      661 gtctttcttt gatcaacagt gaacaagcgg ggagacagga aatagaaatt tttgttgttt
      721 cagaaagttc agtcaaacaa tttttttatc gaacccaatt tcatttgtat gcggaagaag
      781 gcatcgctga aataaatcct attttatttc gttcattaga agaattgcct gatgaatgtc
      841 tatgtgcgaa aaaaagagtg gttttgtgcg atgctgctag ctatcaaaat ggattagaaa
      901 ctgaaaagac aaagatctat ccaatctcat taaaaggtgc aaacatccat ttgtatcata
      961 ttgctcagga acttcaccta ttaaaaaaac atatttcttg atgagacctt aattaggaga
     1021 ccttctacaa agctttttaa ttgtctgtag aaggttttta tttgagtcct gattcgtgcg
     1081 aagatctact tatataacaa gctttctctg aaattttttt aaaatgctat tgacttagag
     1141 tcaactctaa gtagtagaat agataaagtg taacaaatct attttcatta aaagaagata
     1201 gtacggagga gagaatgtat tatgagtaca gtattacatg tattggatgc aatcaatagc
     1261 cccgcagatg tcaaaaaatt atctgttgag gaaatgaaac agttggcaga agagatgcgc
     1321 caaaaaatct tgcaaaagga cagcgatgta ggtgggcacg ttggtccgaa tctaggtgta
     1381 gtagaattaa cgattgcgtt tcattatgta tttgattcac caaaggataa aatcgtttgg
     1441 gatgtttccc atcagtctta tggtcataag cttttaactg gtcgaaaaaa tggttttgaa
     1501 aaaggacatt accgtgaagt gagcggatat tccaaccaac atgaaagtaa acatgactat
     1561 tttacagtgg ggcacacatc tacatctatc agcttagcaa caggtttagc taaagcgaga
     1621 gatctgaaga aagaatcagg aaatgtggta gcatttatcg gtgacggctc cctaagtggt
     1681 ggcttagctt ttgaaggatt gaacaatggt ggtgttttac aatccaatct aatcgtatta
     1741 gtaaacgata atgagatgtc tattgatgag aatcatggca gtttatataa aaacttagct
     1801 gagcttcgtc aaaccaacgg taaaagcagc aataacctat ttaaagcaat gggatttgat
     1861 tatcgttacg tggataatgg aaacgatctg gaaacggtga tccgtttatt tgaagaagta
     1921 aaagatattg atcatccgat tgtacttcac gtccatacgg aaaaaggtcg cggttatcag
     1981 ccggcaatcg ataataaaat gaaatatcat tggcatgtac catttgatct gtccaccggg
     2041 gaggcaaagc aagtaacttc tggagaaaca tttaatatga tcatcatgga ttatttggat
     2101 aagaaaatgc aaacaagtac agctcctatt gccgcagtca atgcagcgat accaggcgcc
     2161 tttggactga aaacattcca agaaaaacat ccagatcatt attttgacgt ggggatcgct
     2221 gaacagcact ctatcacttt cgcttctgct ttggcttctc agggagtacg tccagtcgtc
     2281 ttccaatcaa gtactttctt gcaacgggca tatgatcaat tatcacatga tttagcgatc
     2341 aacgagaatc cagtgattat tttagtcggt ggcggaacga tctcagctag tgaccgaaca
     2401 caccaaggaa catttgacat tccttactta tcaagtatcc cgaacatcaa atatcttgct
     2461 ccagcaacaa aagaggatat gatctctatg ctagattggg cgttcgaaca aacagcgcag
     2521 cctgtagcaa tccgaattcc tacacatggt gtagaacatg gtacagctat cgcaaatgat
     2581 tatagtattc cagcctatca aacagtaaat aatggaggaa aagtagcaat tttagcttta
     2641 ggcggttttc ttgaattagg aaaagaaaca gtaaaagctt ttagaaaaaa aggaatcgaa
     2701 gcaacgttga tcaatccagg aacgatcaca gaaaatgacg tagaaacatt agataaatta
     2761 aaagaaacac acgaattagt catcacatta gaagatggta gtttgtcagg aggatttggc
     2821 gaaaatatca gtcgttatta tggtgattct tcaatgaacg tattaaattt tggtgcagag
     2881 aaagaattca cggatagcgt tcctgtaggg cagctttatg aacgttatca tttaacacca
     2941 aaacaaatcg ttgaagatag tctgcgtgtc ttgaatattc aataattcat tatgctcacc
     3001 atccagataa ttgtttatac tagtatctgg atggtttttt cattataaaa agcaaagggg
     3061 tggatgttat gtatcagcaa gtagaagaat atgtcaaaaa gttccaagaa aaatatccta
     3121 gttatcaatc agaaaaatta ggtttctttt catttgggga tacagaaaaa atggcagacg
     3181 agttgagcga actagttgta aaaaagataa agactggtac atcaagtggt tacgatctgt
     3241 ataaacaaga gaatgagcct ttaccaaaag ttggtcaatt agacgtggtc ctgaatggaa
     3301 aaaatgaacc agtttgtatc atcaaaaata taaatgtgac catcttgcct tttagtgaag
     3361 tcacgaaaga gcatgcttgg aaagaaggag aaggggatcg gacattagct tattggcgga
     3421 aagtccatgc tgagtttttt aaacatgctt ataaagaagc gttgaacata gaatttacag
     3481 aagagaagct ggtcgtatac gaagaatttg aagtaatttt cttttagtaa gaaaggtctg
     3541 ctgaaagaaa tagctcatta ttgtctcctg aatagaaaag ggttacattt ttatatgaag
     3601 ctgtattttt atataacatt actggaggag atattgtgga tacactggaa caaatcaaag
     3661 gcggagtcgt cgtttcttgt caagctttgg aaaacgagcc gctacacagt tcttttatta
     3721 tggggagaat ggctttggct gcaaaagaag gcggagcagt tggtattcga gcaaatacga
     3781 cagaagatat caatgaaatc aagcgaatcg ttgatttacc ggtaataggg attatcaaaa
     3841 gagattacga tgggtcagaa gtgtttatta ctgcgacaga aaaagaagtg gaagaacttt
     3901 tggagacgca agcagaaatc attgcactgg atgcaacttc tcgtaaacgt ccgacagaaa
     3961 aaacaactgc tgaacttgtc caaatgattc acgaacacgg tcggttagct atggcagata
     4021 tttcgacttt tgaggaagcg cttcaagcgc aagaagatgg ttttgatctt ttgtctacga
     4081 ctttagcagg ctatacagaa tattcacgaa agacgcagac acctgatttt gatttattga
     4141 atcaaatcat agaacaagtc gatatcccag taattatgga aggacatact gactctcctg
     4201 aacaagtaga gaaagcatat gaattaggtg ctttttcagt cgtagttgga tctattatca
     4261 cccgtccgca attgatcact aaaagatatg tcgaagccgc aagtaaagca aataactaaa
     4321 aaacacgcac ggcacagatt cttgacaaaa aagaaaaatt gatagatagt ttaaagcaga
     4381 ttctaagaag atgaaaaaag cattactttt cattttgtct tcaagatctg ctttttaacg
     4441 aattttatag aaagaaggcg aaaccatgtt tgaacggagt ccttcagaat tgacagaacg
     4501 tgaaaattac aagttgctga ttggttcagt catccctaga ccagtagcag tagtaacgac
     4561 taaatcagca aaagatatag tgaatatcgc acccttcagt tacttcaata tcgtcagttc
     4621 gaatcgaccc atcttatcct tggcgatcca gagaaaacaa gatgcagtaa aagacacagc
     4681 aaagaatatt cttgagacaa gagaagctgt tatccatatc cttgatgagg acaacgtaga
     4741 agaagtaaat aaaacagcag ccaatctgcc taatgaggaa agcgaactgt acctgactga
     4801 cttgacttta actgattcag taatcatttc agtaccagca ttgacagaag caaaagttcg
     4861 ttttgaaaca tccttatatg aacatgtcga gatacggaat aaaaataaag taacagctga
     4921 tttatttttg ctagaagtac agaaatatca tttaaatgaa acagtctatg atgataagac
     4981 aggaaggatc gatccaagaa aattgaatgc tgtaagccgt ttggcaggaa atgattatgc
     5041 agcaatagga gaaattttca cgattgcacg tcccgaataa aaaggagcac tagttatgtt
     5101 aaaaataagc tataaaccat cgacagattc aaaagaaatg aaaaaagaat atgaaacagt
     5161 gaatgatttt ctgcaagggc agtacttaga agttccgcca ttgcaagacc attttgtcgt
     5221 tactactgtt actttagatg gtaaggaaat agaaatgcca gatcaaacga ttagcggatt
     5281 gtttaattac tttaacaaat aaatatttat tccgaaaagt gaaaaaacat ataaaaaaac
     5341 acataaaact aaaaaattat caaagaatcc tggtgcaact gaaagaattt gcaacgggat
     5401 tctttcaact tatttgttat ttttttctaa aaaatattaa agattgttaa atttcactat
     5461 ttcatttcaa attccattgc ctaatcatta ggaaaagtct atcatgaact cgtaaataga
     5521 atatagtaat aaccttgtta ggtgaggctc ctatacaaac ataggctgtt gcccagaaac
     5581 gtcgaaagtc gccaatgggt aaaacagaaa ctgttggatt aaggcttttt ccaatagcta
     5641 agaatgtttt ctttacgttg tatagtgcta aaactcggac gatgagatga tgctaggtca
     5701 gaattcttgt tcgaaaaggt ttctgacctt ttctttatat ttttgtgact gacttatttg
     5761 atgagacaaa ctcgctcctc acctgacaag tggattgctg tgtgaggagg agatgcggtg
     5821 aaaaaagatc gattggtgga ggtcgctcgg attcacttct ggttgcacaa tacaaccaga
     5881 aaacacaaca agcaaaactg atctctatca tgagagattc atatgtcgat attccaggtt
     5941 acggaatgaa taagataaat gcagcttact cttatggggg agttgattta ttaaatcaaa
     6001 cattaaagga aaatttcaaa tttgaagctc cctattatgc aagtatcaca tttcaagatt
     6061 ttatcgattg cgttaatgaa ctgtttcctg gtggagtcaa gatcgatgca gaaaaatctc
     6121 tagatttaga tggcgtatat ataaacaaag gagagcaagt gatggatggt aatactttac
     6181 tccagtatgc tcgatttcgg gaagacaaag aaggagactt cgggaggatc agaaggcagc
     6241 agcaagtgat caaagctatc tctcagcagc tgaaagacgt aacttcaata ttcaagctac
     6301 ccaaagcggt tgggaaatta cttggtagta tccaaacgaa ccttccagaa agtgtgcttc
     6361 tggattgtgg tatggatttc ctcaaagaca tatgttagaa taaacaggta taaatagtga
     6421 aataaaggaa tgacaagcaa gagaaggagg aaaaaatgaa aagaagtgac aagcacggca
     6481 aaaatcgaac aggcgcttat attgccggcg cagtgatctt aatagcagct gcgggtggcg
     6541 gttattttta ctaccagcac tatcaagaaa cccaagcagt agaagctgga gaaaagacag
     6601 ttgagcaatt tgtccaagct ttgaacaagg gagattataa caaagctgca gaaatgacat
     6661 caaaaaaggc ggcaaataaa agtgcattat ctgaaaaaga gatcttagat aaataccaga
     6721 atatatacgg tgctgctgat gtcaaaggac ttcagatatc gaatctaaaa gtagataaaa
     6781 aagacgattc tacttacagt ttttcatata aagcaaagat gaatacctca ttaggtgaat
     6841 tgaaagatct ttcttataaa ggaacattag acagaaatga tggtcaaacc acgatcaact
     6901 ggcaacccaa cttggttttt ccagaaatgg aaggaaatga caaagtaagc ctgaccacac
     6961 aagaagcagc aagagggaat attatagatc gaaatggtga accattagca acaacaggca
     7021 aactaaaaca attaggagtc gttccaagta aacttggaga tggaggcgaa aaaacagcca
     7081 atatcaaagc gattgcttcc tccttcgact taacagaaga tgctatcaat caggcgattt
     7141 cacaaagctg ggtacaaccc gattactttg tcccattgaa aatcattgat ggagcaacgc
     7201 cagaacttcc agctggagct accattcaag aagtagacgg cagatattat cctttgggtg
     7261 aagcagctgc tcagctgatt ggttacgtgg gagatatcac agcagaagat attgataaga
     7321 atccagaatt aagcagtaat ggaaaaatcg gtcgctcagg tttggagatg gcttttgata
     7381 aggatcttcg tgggacaaca ggtgggaaat taagcattac agatgcagac ggtgtagaga
     7441 aaaaagttct gatcgaacat gaagttcaaa atggcaaaga tatcaaattg acaatcgatg
     7501 cgaaggcaca aaaaatagct tttgacagtc taggaggaaa agctgcatca actgttgcga
     7561 caacgccaaa aaccggtgat cttcttgcgc ttgctagctc tccaagctat gatccaaaca
     7621 aaatgacaaa cgggatctca caagaagatt acaaagctta tgaagaaaat cctgaacatc
     7681 cattcatcag ccgatttgcg acaggttatg ctcctggatc tacgtttaaa atgattacag
     7741 cagcaatcgg tctcgacaac ggcactatcg atccaaatga agtgttgacg atcaacgggc
     7801 ttaaatggca aaaagacagt tcttggggat cttttaaaac aacgcgtgtc agtagtgatg
     7861 tatcacaagt agacttaaaa actgctttga tctattccga taatatatat gcggcacaag
     7921 aaacgttgaa aatgggtgag aaaaaatttc gtacaggctt agataaattc atttttggtg
     7981 aagaccttga tttgccaatt agtatgaatc cagcacaaat ttctaatgaa gatagcttta
     8041 actcagatat cttgctagct gatactggat atggacaggg cgaacttcta attactccta
     8101 tccagcaagc agcaatgtat tctgttttta ccaacaatgg cacacttgtc tatcctaaat
     8161 tgattccaga taaagagaca aaagataaga agaatgtcat cagcgaaaca gcagtacaaa
     8221 cgatcgtgcc agatctgaga gaagttgtgc aagatgtaaa tggtacagca cattctcttt
     8281 ctgctttagg gattccgttg gcagcgaaaa ctggtacagc ggaaatcaaa gaaaaacagg
     8341 atgtaaaggg gcaagagaac agtttcttgt ttgctttcaa tcttgataac caaggttata
     8401 tgatggtcag catgttggaa aataaagaag atgatgattc agcaaccaaa cgagcatcgg
     8461 aactattgca atacctcaac caaaattatc aataaaaaat tgctttttaa gtacagtaaa
     8521 aggactgtgg cgttatcgtc acagtccttt tttcaaataa acggttttca cgatgtctct
     8581 ttcacaactt ctttttgatt tactcacact tcataaactt tttttgtaaa atatttgaga
     8641 acaagtggag aaataagtgt acttaataaa atcacgataa ctaaaggtga atagtactgt
     8701 ggttcgatca attgactttg ctgtccaatt tgtaaaatga tcaaagccat ttctccacgc
     8761 gaaatcatac cagcaccgac catcaatgaa ctatgttgag aaaaaccggc aatctgagca
     8821 ccaaaataac ctccaagcaa tttggtcaaa atcgctacaa gcgtcaatat agaaataaag
     8881 agtagttgtt cactaaagtc agaaaaatca acttctaatc ctacactgac aaaaaatact
     8941 ggtataaata ctgcatatcc taaagcctct acattgtggt atacttcttc tttaaccttt
     9001 gtctgactga cagctatccc agcaaaaaaa gcaccaatga cagagctaag cccaacaaga
     9061 tccgctacgt aagacattcc taaacaaata actagcgata cgatgataat agaagaattt
     9121 gcgtacactt tttctgcaaa tgaaagtagg aaaggcgcaa tccatttgac taataagaaa
     9181 ataaaaacaa aataaattag ctgttcaatc aaaacaagcg gtaaagaagc attgctagaa
     9241 gcttcccccg ttagaaaaga taggctgaaa cttagaacta aaacgactaa aatgtcatct
     9301 accacagaag cacccaaaat tgttgatcct tcttttgtat tcactacgtt tagttctttt
     9361 aatacttcta ctgaaatact gacagaagtt gccgcaagaa taatcccaaa gaaaatcgct
     9421 tcatttcccg aaacttggaa tgctttgcct gtcaaccagc caaaaacaac cgggaagatg
     9481 atcccaagca aagcaacgaa cataccaggt cggaaatatt ttttcaaaag agataaatca
     9541 ctttccagac cagctaaaaa cattaataag atcacaccaa tctccgaaaa gtcatggact
     9601 agaatatcag gtttgaccca attcaatcca gcttggccta gcaaaacacc aacaagtaat
     9661 tgtccaatca ctgctgggat acctaatctc cgcgagatat gtgagccaat cgttgttgaa
     9721 atcaatatca ggcataaaat accaataaat tccattctca tcattccttt gtataaaata
     9781 tagcccgaac ttagcataac atacattatt gaaaaaatag gaaaatatac tcacaaaaaa
     9841 accaacaatc caaaagattt ttttggattg ttggttttta tacggtctat tttaataact
     9901 aaaaatgatt aacgtatagt catttttctt attctgatga aattgataat ctcctggatg
     9961 cccatgaaaa tcaaaataaa gccgaatatt tggaatagaa gcatcaaact tttgaatgga
    10021 ttgaacaata agacgaatcc gccaatcaac aacagaatac tactataaac actccaactt
    10081 ttagcttttg tattcatgct gaagaacaac tggaacaagc tgttgatgat aacagctaat
    10141 ccaagaagaa tcggcaggat agaaacgatt gcaggtgcaa aagcatagac gatcagagct
    10201 aagatcagga aaaaaagacc actgaacaga ccgatactcc aagaacctgt tttcctttta
    10261 aaacggtaag cctcaaaaag attcaacaac ccaagcaaaa caagataggc ggtgatcaga
    10321 taaccgataa aatgaaaaac agcagaaggg tttagcacaa tcgcaatccc agaaataata
    10381 taaacagcag ctcttaataa ggcgtatttt tgaatatttt gtttaatcgt ctcaaacata
    10441 taagaaaacc tcctttgact tattttaacg tgaactagtc attttaccaa attaaatgtt
    10501 acaaaatgag cgaaggagaa gaatatctct ggaaaaatca acttctgagg atcttttcca
    10561 tttttttgcc ttttgctagc tcatcgatca ttttatctaa ataacgaatt tctttcatca
    10621 agttatcctc gatgtcttct actcgtacac cacaaacaac acctgtaatc aaggaacgtg
    10681 aaggattggg gacaggggct tccgcaaaaa attgttcgaa agtcacttgt ttttgtagtt
    10741 gatattctag ttctgtttgg gaataacccg tcagccaaca aatgatttca tctacttcct
    10801 cttttatacg atcttttctc tccgcttttg ctacatacat aggataaacg cttgcaaaac
    10861 ttgtcgtaaa aatacgat
//
LOCUS       DAXLPI010000085        10724 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXLPI010000085 DAXLPI010000000
VERSION     DAXLPI010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.085"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(86..1615)
                     /gene="ahpF"
                     /locus_tag="ACF3T5_001959"
     CDS             complement(86..1615)
                     /gene="ahpF"
                     /locus_tag="ACF3T5_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFD3632558.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1625..2188)
                     /gene="ahpC"
                     /locus_tag="ACF3T5_001960"
     CDS             complement(1625..2188)
                     /gene="ahpC"
                     /locus_tag="ACF3T5_001960"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFD3632559.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2332..2994)
                     /locus_tag="ACF3T5_001961"
     CDS             complement(2332..2994)
                     /locus_tag="ACF3T5_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3632560.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3178..4824
                     /locus_tag="ACF3T5_001962"
     CDS             3178..4824
                     /locus_tag="ACF3T5_001962"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFD3632561.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4815..5897
                     /gene="uxuA"
                     /locus_tag="ACF3T5_001963"
     CDS             4815..5897
                     /gene="uxuA"
                     /locus_tag="ACF3T5_001963"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFD3632562.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(6005..7558)
                     /locus_tag="ACF3T5_001964"
     CDS             complement(6005..7558)
                     /locus_tag="ACF3T5_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632563.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            7706..8995
                     /locus_tag="ACF3T5_001965"
     CDS             7706..8995
                     /locus_tag="ACF3T5_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFD3632564.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVILWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(9121..10200)
                     /locus_tag="ACF3T5_001966"
     CDS             complement(9121..10200)
                     /locus_tag="ACF3T5_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFD3632565.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(10309..>10724)
                     /locus_tag="ACF3T5_001967"
     CDS             complement(10309..>10724)
                     /locus_tag="ACF3T5_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFD3632566.1"
                     /translation="VCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLVLWEKKKTSV
                     KELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAALQKQAEGIP
                     QKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
BASE COUNT         3607 a         1967 c         1952 g         3198 t
ORIGIN      
        1 ttattcaaaa aaacaaaaag gattgtgaca aaattcttgt caccatcctt ttttccgctc
       61 tctgactttt tacaatcttc atagctcaag tgcgcatcag ataatcgaat gctcctaaag
      121 ctgcagtagc tcctgaaccc attgaaacaa tgatttgttt gtatggacta tctgtacaat
      181 caccagctgc gaaaacgccg gggatattcg ttgcgccatt tttctcggtc actatttcat
      241 ttcttggatt catttcgatc gttccatcta gccaatccgt attagggagt aagccgacta
      301 gaatgaatac tcctgaaaca gctagtgtat gcatctccat cgtacggcga tcgatatagg
      361 agagagattc tacctgattc gtaccgctga tttctttcgt cgctgcatgt ttgataacag
      421 tgacattcgc aagagagact agtttatcct gtaagatttg atctgctttt aattctggta
      481 agaattctag aacatataca tgttttgcta acccagccaa atcgattgct gcttcaacac
      541 cagaattacc accaccgata acagcaatct cttttccttt aaataaaggg ccgtcacagt
      601 gtgggcaata agcaattcct ttatttttaa actctttttc gcctggtaca ttgatagaac
      661 gccagtgcgc acctgtcgcg atgatggcgg tttttgcttt aagtgatata ccgttagcta
      721 agccaatatt aataaaatgt tgctctttat ttaaggaaac ggcctgttga ttcatcataa
      781 tgtcgatagg gtaactacgc acatgacttt ctacttgctg catcaatttt ggaccttctg
      841 tgtaagaggt accaatcata ttttcaatcc ctagtgtttc aacgacttgt ccgccgaaag
      901 aatcagcgac aagtcctgta cggattcctt tacgtgctgc atagatagct gcactactag
      961 ctgctggtcc accgccaatt actaatacat cataaggttc tttttcaaac aactcttctt
     1021 caatcaaagg gccagtgatt ttttcaacaa tttgttcgat cgtcatgcgt ccgctactaa
     1081 attcttctcc atttaaaaac acagttggca ctgccatgat ttttttatca tcgatttctt
     1141 tttggaacat cccaccttca atcatcgtat gagtaatatt tggatttaat acagctaata
     1201 tattcaatgc ctgtacaacg tcggggcaat tatgacaagt caagctaaca taactgtcaa
     1261 aatgcaatgt ttgtgttatt tttttgattt taagactcaa agattcactg attttcggtg
     1321 cccgtccact aatttgtaac atagccagta aaaaagagga aaattcatga ccgaggggga
     1381 taccagcaaa aacgattcca ctttctttgc caactggatt gatagaaaaa ctaggtgtgc
     1441 gcatcgaatc tcttttttcc caagagattt taggagacat ttctgctatt tccttcgtaa
     1501 attcagctag tttttgacta ttttcactat tatccaaact aacagaaaaa acaataggag
     1561 attcaatcag tgctaaatat tgttcgagat tttgtttagt ttctttgtct aacatatcca
     1621 ccacctaaat ctttccaaca agatcaaaac ttggtttcaa tgtttcgcca ctttctttcc
     1681 attttgccgg acaaacttca cctgggtggg tacgtacata ttgagcagcg cgaatcttat
     1741 caatcagtgc actagcgtca cgaccgattc catctgcatt gatttccata gcttgaacga
     1801 ttccgtctgg atcaataata aatgttcctc gctgagctaa tccttgctct tcatccaaaa
     1861 catcaaaaat acgagaaatc tgatgagacg gatcaccgat catcacgtat tcgattttac
     1921 cgatagcatc tgatgtatca tgccatgctt tgtgagtaaa gtgggtatcg gtagagcaag
     1981 aaaagacttc aacacctaaa gatttcaatg tactgtattg ttcttgtaag tcttctaatt
     2041 cagtaggaca aacaaaagta aaatcagctg gatagaagca aagaatactc cagttcccca
     2101 taagatcttt ttcactgact tctaaaaatt ctccttgatg ataagcatgt gcattaaatt
     2161 cttcgatttt tttcccaatc aatgacatag taaaacctcc aataataatt atttatttat
     2221 aattattgta atttagttgg agttgcaaag tcaatggata ttttgttatt taataaagat
     2281 tactcaaaaa cgaaggttaa ttataaaaat agaagaggtc gcaagaaaat gctactgctg
     2341 gaaatactcg ggataacgtt gagtaacagt ttcctgttct ttcagcatca tgtgtaagtg
     2401 caatcccgtt tgaaatcgag cttcttctgg ttttttatca actaaagcct tgagtaaatc
     2461 ataatgttgt tccatgatcg tttcccatgg aagcccctga atggtcactc gtaaccagcg
     2521 gaaacgttct aaatgggtat tatggtcatc taaccagttc caaacttctt ttcttcctgc
     2581 aatttcgaaa caagcttcat gaaataaatt atctgcacgg aaaaaagcgc gttcgtcatt
     2641 tattttgact gctttttctt gttcttctaa aatcgtttct agcactattc gtgtttgtgt
     2701 gtccaatttg gaacaacatt caatcatgat ttgctcttcc aactgttctc ggacaaagcg
     2761 agcattttct gcagaaacaa gattaatttt ggaaacatac gtaccacttt gaggtatcgt
     2821 atacacgagt tcttgctgac gtagttgaat caatgcttcg cgaatgggag tccggccgat
     2881 agcaagatgt tcctccagtg ccttttcaga tattttcctt ccaggttcta agtcagcata
     2941 gataatttgt ttacgaatcg cttgataagc ttgtgattgc aagctgcttt tcatctttat
     3001 tcctccaaaa tcatttctat gaacgtagta taacagaaga ggtctgaaaa acaatctggt
     3061 atgtcacatt aactagtaaa tttgtataaa tagtaaaatt ttggttgaca tagcgatttt
     3121 tattgtataa aattcacttg aatacaaaaa ctaatatgtt agtttaggag gggaaaaatg
     3181 attgatttaa acaactggag gaactttatc cctgaattat ctcaaaaaga agtagaaata
     3241 cctcattttg accgtgcatc attaagagaa aatggacaaa aaaatcctgt ctggatccat
     3301 ttcggtggcg gaaatcttta tcgaggattt catgcagaaa tcgctcaacg tttagcggat
     3361 ctaggcgaat tgtccactgg tgtcattgtt tgtgaaacat tcgatgaaga aataattgaa
     3421 agggcttacc aagattttga tgatttgatt cttgaagtgg tcatgccaag cagtggagag
     3481 ttcgtcaaac gtatattagc tgcgacagct gacagtctgt attgtcatcc agattgttct
     3541 gaacactttg ataagatgat tcgacacttt gaaaatccta gtttacaatt agttactttt
     3601 gctattactg aaaaaggtta tcgattgaaa gatacaaata ataaatatat aggaccgatt
     3661 gcagtcgata taaaaaatgg tccaaagaag ccatcacata caatgagtat tcttgctgcg
     3721 ctactttata aacgtttttt atctggagct tggccgattg cacttgtctc tacagataat
     3781 ttttcacaaa acggtagtca attacgtact tctttgctaa cgatcgcaaa agaatggcaa
     3841 aaggaaggtt atgtacaaaa tacatttatc caatacttat cgaatgaaga aattgttgcg
     3901 tttccatgga cgatgattga tcgtatcacc ccgaatcctt ccttgtcagt tagcaaaatg
     3961 ttggcaaaag agggcttttc aaatatgtcg attttccaaa ccgcaaaagg aaataatatt
     4021 gctccatttg tcaatacaga ggacatccat tatttaatca tggaagatca ttttcccaat
     4081 ggacgtccga aattcgaaaa agctggggta ttcttgactg atcgtgcaac ggtagataaa
     4141 gcagatacga tgaaggtgac gacttgcctt aatcctttgc atacgtcttt agctattatg
     4201 ggttgtttac tggggtatag gagcatagct gaggagatga aagatccaga tttatcgctg
     4261 ctagtttctg aaatcgggta caatgaaggc ctcccagttg tggagtctcc aggtatcatt
     4321 gatccaaaga tctttctgga agaagtaatt actgtgcgct tttctaatcc aaatattcct
     4381 gatatgcctc agcgaatcgc cacggatacc tctcaaaagg tggcgattcg ttatggtgag
     4441 acgattaaaa aatatatgga aatagaagtt aaagacccgc agacacttgt attcatacca
     4501 ttgactctcg ctgcttggct tcgatacctt attggcattg atgatcaagg aaaaccattc
     4561 atcccgagtc cagacccact attgacagac tttcagcata agctttccga tattcaactg
     4621 ggtacgcaag atacatcaca tattcatgag aaaatggctc ctattttgtc aaataaagaa
     4681 atttttggtg tagatcttta ccaagcagag cttggcagaa aaatagaaga aatgttcatc
     4741 aagatggtag caggaccgaa tgccgtacga aaaacattac aggattactt acaagaacat
     4801 ggaggaaatt tatcatggaa atgactttta gatggtacgg atcacaggaa gaaaagatta
     4861 ccttagatga aatcagacag attcctgcgg tcaaagggat tgtaggcaca ttgatggata
     4921 taccagtagg agaggtatgg cctagggaga ggatcaaaga actaaaagaa gaaattgagt
     4981 ctcgtgggtt aacactaaaa gtaattgaaa gcgtcaatgt gcatgatgcc attaaaattg
     5041 gattaccttc ccgtgagcag tatatcgaaa actataagca gacaatccga aacttggcag
     5101 aatatggaat agaagtcatc tgctataatt ttatgcctgt atttgactgg gtgaaatctg
     5161 accttgacta ccgcttagaa gacggctcat ccacgttagc ttttatatct gatgatattc
     5221 cagatgatcc aagagaaatc gtcgcgcgca tcgaacattc cagcaatgaa tttgaattgc
     5281 cgggatggga acctgagcgc ttagctcata tcaaatcctt gcttgaagcc tatgcatcag
     5341 tagacgaaga aaaacttcga gaaaattttg cctattttct ccaatcaatc atcccaacat
     5401 gtgaagaggt aggggtcaaa atggctgtac atccagatga tccgccatac tcgatttttg
     5461 gattacctcg aatcgtaaag aatcgagaag atttagattg gttgtgccgc atagtagaca
     5521 gcccttataa cggaatcact ctttgtacag ggagtattgc agaagatcca gaaaatgatg
     5581 tatatgggat tttagcagaa ttttgtcaaa gagatcgaat cccatttgca catgttcgaa
     5641 atattaaatt tttagctaat cgagatttct atgaggcacc gcataaatcc gaatacggtt
     5701 cattagacat gtaccgcata ctaaaaacaa tgtatgacaa cggatttaac ggatacatcc
     5761 gtcctgatca tggaagaatg atctggaatg agacaggaag accaggatac ggcttatatg
     5821 atcgagcatt aggtgctgtt tatctaaatg gattatggga agcaatcgaa aaaggaaaca
     5881 aaagtaaaga taattagtga aaaaaactgt aattttatta tgtaaataag aaggatgttc
     5941 aaattcttga tacataaatc cggaggttgt taataagcat tcggatttat gtatcttatt
     6001 tggtctagga agtaacttga ttttcttttt tcgattgatc catgagtgct tcttctttta
     6061 attcaaagat atattctgag atcctcataa tcactttttt ccagtcttct ggtgtgatga
     6121 ttccttttaa aatatagatt tttttgatat gagaaggcaa ttgtttcaaa gggcagtcgc
     6181 tgataacgat gttgcagttc tccataaaat cattttgtat tttgatcgat tcatttgaaa
     6241 aaatttgttc tatcttaacg atcagatctt tactgatata aaaatcacgc gtatagttca
     6301 caccaatcac aattcgcggc tgttttttgt agcgatcata caaatgtact aggtcttcaa
     6361 ccatccattg cacattttcc ttttctaaaa taactggtaa caagtgttga ttttgttctc
     6421 tgaaatccat ataaaattgt tcaatggcgg tttggacttc tttcaacacg ctatgttctt
     6481 gataaagcat gaaagtagga aggggcgtat ttggttgatt gaagttaaga aatccattat
     6541 aaagtttatt tcgtgcgaat gccaatgagt aaagaatctt ttctttttca ggtataaaca
     6601 aatcaaatac agtacaaaag gactccacca tttctcttga ataaagaaca tgaggaacgt
     6661 ttgctttttt taaaagtttg tattgctcca agcttgtttc ttccgattcg attttcgtga
     6721 tagaaagtcg agctaaaata ttcactaaga tgcgttgttc gcgacaaatt ggcagattag
     6781 gtcgcaaaat atctacagag ggatcaataa aaaagtcaat atattcatac tctgtgaatt
     6841 ctttttgtac gtctttcaca gaagttgcag gaaaattctc tttacatatt tttaatagaa
     6901 aatataattt atctatcata ccgctattta gtcggtcaag tacttctttt ttaaaaaaag
     6961 tattcatcat aaaagccact acttgcggat cttcatcata aaattctgta gcagtacttg
     7021 aaaagatgtt ccaataaata tctaatgctg catattgaat ttgtacctct tttccagaaa
     7081 aagactttct gccattatta gaaaaggaga tagacaaacc gtacaatgct aaaaatgcat
     7141 taatcttttt cattttcgta tacaaataag aggcactata gtttgtttgc tgacttaaac
     7201 tgataatcga atttgtctga cagctgaaca aagaagtcaa gatctgataa ctgactgatt
     7261 cgttgcagta tcttccaaat agacgataat agcagtaagc cggatcaatt ccttcaaaat
     7321 tcaactttaa aaaaggtttt tgcttatcaa tatgcagttc tgttagattg agacgctcaa
     7381 agtcttgttc taattctttg atgtatagta aaaaatttga ttcagacata tggaaatgat
     7441 ctgctaaata ttgtgtagta ataaagaaac gatgttcaga taggaaaaga aaaatatcta
     7501 attttgtatt gctttttttc ttcaagagaa acgttttaat cgcttcttga gtttgcatga
     7561 aagaggctcc tttttactta aattgttaaa acttgaaaat attactgttt ttttcataaa
     7621 tttagacaaa aaaacagtaa acatataggt agaatctcta ctatcttatt tcttgcatac
     7681 aaaaaaatca aggagtgaga gagttatgag atattcaacc atcttaatat caacatttgc
     7741 agccttaagc ttatcgctta ctataaatca gacagtccac acagaggaga tagaaacagc
     7801 caaatggaca gaaaccaagt gggccaacgt caatcgaatt acctttcagg atgaattttc
     7861 atccttcaat tcaggagtcg caaaattaag aaaaggacaa acagcgacca tcaaagcaaa
     7921 aattgactat tcaggagact tagcacctat actacaatca gctgttacat ttgagaatgt
     7981 tgatcccgct ttgtcgattg gctatgacga gaacactttg actatccaac acaagcaagc
     8041 gatatttacc ttcaccgtta cattaaatca ggacttgtta aaaccagcgc tttttactgt
     8101 caaggtatca gatggactac cgaacgatca tcatcattta acgccgtata gtcagcggca
     8161 gacaattgag caagattcag ctatcgactc aggaggagat cttgttgaag aaccaacaga
     8221 taaaccagaa aatgaaaata agccagaagt accgcctaca gaaaatccag acggagaaca
     8281 gaaaccggaa atagaacctg gagaagaacc cgatacagaa actcaaccag agccagacaa
     8341 tgaatcaaaa cctgaaataa cccctggaga aaaaccagat gtagacccag aagaaaaacc
     8401 ggatgtgact ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc
     8461 agacacagat aatgaaactg aaaatccaga aaaaccagag gtagccccag aagaaaaacc
     8521 agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg aaacaaatcc
     8581 agacacagat aatgaaactg aaaatccaga aaaactagag gtagatccag aagaaaaacc
     8641 agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag aaaaaataaa
     8701 caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc tagcaattct
     8761 aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg cccaatcagc
     8821 tgaaacctta aaaagtagca aggatacaac ctttgctagt cctgaaacga aaaataaaca
     8881 gctaccaaaa tccggagagt cacaaaacaa agttatactg tggagcggaa tcattctatt
     8941 aagtatagca acaatgcttt ctgcaaaaag atttaaacaa aacagatctc tctaacattt
     9001 gaataatgtc tgtgaacgca tttttcaaat aaaaagaaat atccagaaaa cgaccccaaa
     9061 ataccagatt gaaatctgat atttggggtc gttcatactt tatctttctt catatttatt
     9121 ttaagggata tctgtttctt ttggaagatg gttcgggatc tttttcactt tcaatacatc
     9181 taaaaagaag gtaaagatcg gaatgccgac aatcaagccc caaacgccaa agaaccgttc
     9241 gctaacaagt aagaccacaa atgtataaaa aatcggcagt tctgttttgc tggacatcaa
     9301 cttcggattc agcacataag actcaattag atggacaatc aaaatcgtaa tcaggatata
     9361 aacaacatct cggatgcctc cttgggaata agctatgaaa gatagaggaa tacaagaaat
     9421 gataacacct gctactggga tcagacttaa aatgaaaatc atgatcgcta aactcagcag
     9481 ctgatgaaag ccaaatacag ccaaggcgat tgtagtgagc aacgtgttta aaagagcgat
     9541 aacaaattga gcttctaata ccaaaccaaa tgtattgaca aacttatcgg caaaatagta
     9601 aatatcttga aagaaccaag aaaattcact ttttaaaaac aatcttgaga atacaattgt
     9661 ttttttcttt tcaatcataa aaaagaaact taagatgaat gacatagcaa acgccactgc
     9721 catttttcct atatcctgta aatagccaag cgcaatcgaa gcgccatttt gaagctgttc
     9781 aaaaaaatcg tttcgctcaa tataagtatg gatatattgt aaaacaggat ctgcatcttt
     9841 tggctgatgc tcgtaaaaat caataacgga ctggatcata tcaaatgttt gatgaataag
     9901 catcgggata tactttgtga atgccagata gataaggtaa acgatcagtg tataaatcat
     9961 gatagctaaa acacctgtag ggattcggac aaatcgctga atgatttgaa caagatgaat
    10021 catcaaataa gtaaagataa atgtgagcaa aatggttgta atcatacttc taactaagaa
    10081 aagaacaaaa acgacaaata ataaaacagt gagacggcgt attttttcat tcgccaaaaa
    10141 acgctgataa acggtcaaat cgacaacctc caaataacaa aaaataaaca cgttattcat
    10201 tatatgatgg aatatgaaaa taagaaagag aaagagcaaa aagaaaaacc gctaaatgac
    10261 gttatcttat gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt
    10321 ctggtcttga tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc
    10381 ttcctgttca agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg
    10441 caacgcagca ccttgaggag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg
    10501 ctgacgatta atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga
    10561 atccagacac aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac
    10621 taacatcaca agatactggg tataggtaag attcagcggc ttgaggaggg aagtgtagtg
    10681 tctaatgatt tccttagagg cggcatataa aggaaaacat actt
//
LOCUS       DAXLPI010000086        10595 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXLPI010000086 DAXLPI010000000
VERSION     DAXLPI010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.086"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            12..84
                     /locus_tag="ACF3T5_001968"
     tRNA            12..84
                     /locus_tag="ACF3T5_001968"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="ACF3T5_001969"
     tRNA            88..160
                     /locus_tag="ACF3T5_001969"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="ACF3T5_001970"
     tRNA            168..249
                     /locus_tag="ACF3T5_001970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="ACF3T5_001971"
     tRNA            267..339
                     /locus_tag="ACF3T5_001971"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="ACF3T5_001972"
     tRNA            351..422
                     /locus_tag="ACF3T5_001972"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="ACF3T5_001973"
     tRNA            439..524
                     /locus_tag="ACF3T5_001973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="ACF3T5_001974"
     tRNA            535..608
                     /locus_tag="ACF3T5_001974"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="ACF3T5_001975"
     tRNA            618..691
                     /locus_tag="ACF3T5_001975"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="ACF3T5_001976"
     tRNA            711..783
                     /locus_tag="ACF3T5_001976"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="ACF3T5_001977"
     tRNA            811..884
                     /locus_tag="ACF3T5_001977"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="ACF3T5_001978"
     tRNA            898..971
                     /locus_tag="ACF3T5_001978"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="ACF3T5_001979"
     tRNA            995..1084
                     /locus_tag="ACF3T5_001979"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="ACF3T5_001980"
     tRNA            1097..1170
                     /locus_tag="ACF3T5_001980"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="ACF3T5_001981"
     tRNA            1189..1264
                     /locus_tag="ACF3T5_001981"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="ACF3T5_001982"
     tRNA            1266..1336
                     /locus_tag="ACF3T5_001982"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="ACF3T5_001983"
     tRNA            1350..1423
                     /locus_tag="ACF3T5_001983"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="ACF3T5_001984"
     tRNA            1448..1535
                     /locus_tag="ACF3T5_001984"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="ACF3T5_001985"
     tRNA            1557..1628
                     /locus_tag="ACF3T5_001985"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="ACF3T5_001986"
     tRNA            1651..1740
                     /locus_tag="ACF3T5_001986"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="ACF3T5_001987"
     tRNA            1753..1826
                     /locus_tag="ACF3T5_001987"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="ACF3T5_001988"
     tRNA            1845..1917
                     /locus_tag="ACF3T5_001988"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="ACF3T5_001989"
     tRNA            1921..1993
                     /locus_tag="ACF3T5_001989"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="ACF3T5_001990"
     tRNA            1998..2068
                     /locus_tag="ACF3T5_001990"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="ACF3T5_001991"
     tRNA            2081..2154
                     /locus_tag="ACF3T5_001991"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="ACF3T5_001992"
     tRNA            2166..2237
                     /locus_tag="ACF3T5_001992"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /gene="metK"
                     /locus_tag="ACF3T5_001993"
     CDS             2503..3696
                     /gene="metK"
                     /locus_tag="ACF3T5_001993"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFD3632567.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="ACF3T5_001994"
     CDS             3826..5298
                     /locus_tag="ACF3T5_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFD3632568.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="ACF3T5_001995"
     CDS             5381..6169
                     /locus_tag="ACF3T5_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD3632569.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="ACF3T5_001996"
     CDS             complement(6236..6904)
                     /locus_tag="ACF3T5_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFD3632570.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="ACF3T5_001997"
     CDS             7019..8731
                     /locus_tag="ACF3T5_001997"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632571.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="ACF3T5_001998"
     CDS             8734..10503
                     /locus_tag="ACF3T5_001998"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632572.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
BASE COUNT         3045 a         1937 c         2456 g         3157 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttacc
//
LOCUS       DAXLPI010000087        10595 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXLPI010000087 DAXLPI010000000
VERSION     DAXLPI010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.087"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(29..727)
                     /locus_tag="ACF3T5_001999"
     CDS             complement(29..727)
                     /locus_tag="ACF3T5_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFD3632573.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(720..2072)
                     /locus_tag="ACF3T5_002000"
     CDS             complement(720..2072)
                     /locus_tag="ACF3T5_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFD3632574.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2295..3695)
                     /locus_tag="ACF3T5_002001"
     CDS             complement(2295..3695)
                     /locus_tag="ACF3T5_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFD3632575.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3856..4593
                     /locus_tag="ACF3T5_002002"
     CDS             3856..4593
                     /locus_tag="ACF3T5_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFD3632576.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4718..5197
                     /locus_tag="ACF3T5_002003"
     CDS             4718..5197
                     /locus_tag="ACF3T5_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFD3632577.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5194..5961
                     /locus_tag="ACF3T5_002004"
     CDS             5194..5961
                     /locus_tag="ACF3T5_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFD3632578.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6191..6760
                     /gene="lepB"
                     /locus_tag="ACF3T5_002005"
     CDS             6191..6760
                     /gene="lepB"
                     /locus_tag="ACF3T5_002005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFD3632579.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6864..7466)
                     /locus_tag="ACF3T5_002006"
     CDS             complement(6864..7466)
                     /locus_tag="ACF3T5_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFD3632580.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7572..7802
                     /locus_tag="ACF3T5_002007"
     CDS             7572..7802
                     /locus_tag="ACF3T5_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632581.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            7793..8041
                     /locus_tag="ACF3T5_002008"
     CDS             7793..8041
                     /locus_tag="ACF3T5_002008"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3632582.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            8147..8731
                     /locus_tag="ACF3T5_002009"
     CDS             8147..8731
                     /locus_tag="ACF3T5_002009"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFD3632583.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8734..9807
                     /gene="prfA"
                     /locus_tag="ACF3T5_002010"
     CDS             8734..9807
                     /gene="prfA"
                     /locus_tag="ACF3T5_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFD3632584.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9800..>10595
                     /gene="prmC"
                     /locus_tag="ACF3T5_002011"
     CDS             9800..>10595
                     /gene="prmC"
                     /locus_tag="ACF3T5_002011"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFD3632585.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIK"
BASE COUNT         3636 a         1907 c         2086 g         2966 t
ORIGIN      
        1 ttttgtcaca ttcccctttt tcgaataact aggctgtttg tccttcaaga tagggcagat
       61 ccaattcttt accgtaacgt tgttctaaag ctaggcggat caaacgagct gccaggttct
      121 catttcttgc aaggataggt ccatggaaat aagaaccgaa gacatttcgg taaatgacac
      181 cttcagattg gtcttcacca ttatttcctt gaccttgtac cactttcccc aaaggtcgtt
      241 caccttcacc taagaaagtc cgaccattgt ggttttcaaa tccataataa gtttcattga
      301 attcttcgtt atggataacg atatcaccga taaaccggtt attttcctga cttaacgtgt
      361 agtgatccag tgcactgatt ccttggatct tttctccatt agctcccatg tagtagtgac
      421 ctagcaattg gtagccacca caaatggcta acatgacgcc atcattttcg atatagttga
      481 tcaaagaatc tttctttttc tggatgtctt ttgaaatgat taattgctca aagtcttgac
      541 caccgccaaa gaaaaccaag tcatatttat cagcatcaaa aggttcatgt atactaacga
      601 tctccgtatg gcaagaaacc cccatttttt ctgctatata tttcaacatc aaaaggtttc
      661 cgttatcacc atatgtgttc atcaaatttc cataaaggtg ggcgatattg agttcataat
      721 tagccacggt tcattcctcc tttgataaat ccttgagcag ctagttcttt acgaagctgc
      781 aatacagcag tgtaagtagc taaaatatag acatgctcag ttggcaattc tttgattttt
      841 tcgattactt ctggtaattc ttttgtttct gtcaatttat tttctggaat cccggcaact
      901 tttagacgta aagtcatatc tgtatgacgg tctcctccag aaataacagc tgggatatcc
      961 atatcggcaa agttttcaaa attaccatcc caaatccagc tgacatcgat tccgtctgcg
     1021 taattggcat ttaataaagc cactaaagaa aatggataag gagctagacc aatcatatcg
     1081 attacttgat tgatacctac aggatttttg acaagtacta atgtacattt tttcccattg
     1141 atattgatga cttcttgtcg gccgaatact ttctcgtcat atcctaaacc ttgtttgatt
     1201 ttatcaggat cgacattgaa gtactctgcc actgcagtag ctgctaaagc attgtagaca
     1261 ttatacatac cgccaacttc gattccgtat tcatgaccat cgatcacgaa atctgctgac
     1321 acattgtcca tccgtaccat atcagttaat cgataatcca attcaggacg tttgaagtca
     1381 caatttggac agtaatattt ccctaagttt gcataagtga tcattttata atgcaagatg
     1441 tgatgacaat ttggacataa gacaccgtct gtattgtaat gagccatttg ttcacgatct
     1501 tcttcatggt caaagccata atattttcta ggattgactg tttcgataga attgaagatt
     1561 ggtgaatcac cgttactgat aatcgttgca tttggtgact ttgcggcacc gtctacaatc
     1621 attttatagg tcgtatagat ttctccatac cgatccattt gatcacggaa aatatttgta
     1681 aataagaaca gttctggttt catatattct gtgactttgc tcaaacttgc ttcatctatt
     1741 tctaggacag caaacttttt ctgtcctttt tttggttttg cctgcaagaa agtcgataca
     1801 attccctgta ccatattcgc accagtaggg ttggttagaa cttcatcgaa ttgctggcgc
     1861 aaaatattga ctgttaaagc ggtagtcaat gttttaccgt ttgttccggt gacaacgacg
     1921 acttggtagt cttttgccaa actatctagt atatgtggat cgactttcag cgctagttta
     1981 cctggtaaac ttgatccgcc tttaaaaaaa gtttttaatg cccattgcga cgtttttcca
     2041 actgcaatcg caaaatgact gcgtattccc attatgtaac ctccaaaaac gatttgctga
     2101 cgaataaatt caactaatca taccatatgt tgaaaaaaag gttaaattgc ttttcttatt
     2161 cttaagaaaa ggttacggct gaaagtgcat gaaatcggac aaaatcgcat gatttaacaa
     2221 aaaaacagct tgaaacaaaa tgattgtttc aagctgctgt gattttatta ttccctctta
     2281 cctatattcc cattttaacc gataataatt ttctcgcttg gatattcata gcctagatct
     2341 cgtgtttctt ttggcacgaa taacatcaat gtatatagca ttccgatccg cccaataaac
     2401 atcagtgtag caatcgtgat tttgcccaca acagacaagt ctccggtaat ccctaaagat
     2461 aaaccagttg ttccaaacgc agaagttact tcgatgatga tcgagatcag cgtttgttct
     2521 tctgtagcag ataaaaaaac gatacaaaag aaacacatac caagagaaag cataaagaca
     2581 acgactgatt ttcgcacatc atcttgatcg attctccgtc caaaaatatt gatgttatct
     2641 tcacttttga ggaaggaata tagatacaaa ccgatgattg ctacagtagt tgttcgaatc
     2701 ccgccgccta cagaactagg actgcatccg ataaacatca gtaaagaaaa aatgatcagt
     2761 gttgtgattt gaaaatcacc caaatcgtga atctgcagac cagcattacg agtcgtaatc
     2821 gaataaaaca tcgaattgat ccatttgaca ctcatattgg aatcttggaa taaatggtcc
     2881 ttttccaata gataaatcaa gacagttccc gaaacaaaca agataacaaa agctaacaca
     2941 gctaatttgg taaacaagga aaaacgaaac ggaagcttcg cattggaacg tttgtataat
     3001 agccattccc ggcactccat caatacagga aaaccaattc ctccgacaaa aatcaaaaac
     3061 atgatcagga tcaagaaaaa atagtcatgt gcaaagggct tgatggaatc cccagtgacg
     3121 tcaaacccag agttcgtgac agccgatatc gcctgataaa aaccgtaaaa aacggcatca
     3181 cgccaacgat caaaatagcc tctgtaataa aaataaatag aaaagaatgt cccaaaaagt
     3241 aattgaaacc agattaaaat agcaaaagtg atccgaatca gtcggacgat accagacaaa
     3301 cgcggttgat tcatatccgt catgatcagc tgtcgctgtt tcagtgtgat ccgtcgttta
     3361 gaaaaaatga caaaagcagt agaaatcatc atgatcccca aaccaccaac ttgaaataat
     3421 atttccagca gtataatacc gttatcattg aacacagaat tgatatcaaa agtcgatagt
     3481 ccagtcacac tcactgtact gattgccatg aacagcatgt ccacaaaaga aacatggcta
     3541 tctggttcgc gaaaaatcgg catataaaat agaacgagtg ataagaccgt catcaatata
     3601 taataaaaaa cgatgatttg gatcgatgag aaatgattgg cagcaaactt ctgtcctcgc
     3661 cgctgcagcc ttcgaaagaa taaatcaaca tgcacagtac tatcctcctg acaatagtcg
     3721 tttctaccag tatacaaggg gaattaggaa tactcaaaga aattatcaaa cagataacaa
     3781 tacagctttc gattaaaata gaaatggctt ataataagga aaatcctaga aaaaagaagg
     3841 aggaggaata acaaattgat taatgctcaa caaatcgtag aaaaaatgaa aaatcaaaaa
     3901 ataaattatg atacagttct gaaaaaaatg atcatgcaat gggagaaaga agaagtaaag
     3961 cctaagatat taatacatac atgctgtgct ccttgcagta cgcacacatt ggaattcatg
     4021 tctcaatatg cagagatcac gttatttttt tcaaattcga atattcatcc taaaagtgag
     4081 tatttgcgaa gattacatga acaaaaacga tttgtagacc aatttaatga gaaaactgac
     4141 catcatgttg cgctaatcgt tgatgaatac cgtccaaatg aattcaccaa aatggtttta
     4201 acaaaccatc ttgaaaacga aaaagaaggt ggtgctcgct gcagtgcatg cttcaatatg
     4261 cgattagatc ttgttgcgca aaaagcgcaa gaactaggat acgattactt tggaagtgcc
     4321 ctgaccatct cgccaaagaa aaatgcagct ttgatcaatc agatcggaat ggatatccaa
     4381 aagatttacg ggacacaata cttgccaagt gatttcaaga aaaacaaagg ctatgaacgc
     4441 tctttgcaaa tgtgcaaaga gtatgatatt tatcgccaat gttattgtgg ttgcgtgttc
     4501 gctgccaagc agcaaggatg cgatctgaaa gaaatcaatc gggaagcaaa agcttactta
     4561 aaaacatcaa ccgtagtttt tgaggaaaat taaagaaatc tttcggaaaa acgtaagaaa
     4621 aaaataaaga atttatggta gaaataaggt agactgtata atttgtccgt agtaaagtaa
     4681 ggaaacaaga ataaatttca atgaaggaag agataagatg cgaaaagtag cacctatttt
     4741 gaagccaatc gctgtattaa caatctcgac gattgtcgcg actactacag gtattgtgaa
     4801 tagttccagc taccgtgcac caagttcaag ttctagtact gtagaagaaa caacaccaac
     4861 tacttcagtc tcgacatctg aaccaacaac aacggaaagt ttgaatgaaa ccactacgac
     4921 aagtacatgg gacgatacaa caactagttc gtcgtatgat gagacatata caactgacag
     4981 ctcaagcgaa acaagtaata cggtaccaga tggcactcca tcggattcaa ccaatccaag
     5041 tacaagtccg gatacaagtg ctagctccga aacgatacca agcagtacga ctgtagatga
     5101 taacacgcaa aatgatgatg cagcacgtac agcacagcaa aaccaagatt caggaaatac
     5161 ccaaacgtat aaccagcaag caggtgattc tcaatgacat ttttactaga ccttatcaac
     5221 ggggtgcata aattccttgg ctatttagac atcagtccta aatatcttaa ccgtggatat
     5281 acgattttaa gtgtgattcc cacgctttat ttattgagaa ttgtttatgg actttggcaa
     5341 aaccaaaact atcttcaatt tttcttatat ggacttgcat ttctagtgct tgtttatttt
     5401 actgttttaa atgtatttta ttacttttta gataaaaaca cgaaagccga tgtcactcag
     5461 ctttttgtga agtatttacc tgatgaagcg ttcaacatcc agacagaaga atcgatagat
     5521 aatggaaatt cgatcgataa agtgaataca caagaggtca tggtttctta tgacgaagat
     5581 tatcaattga aattagccga gaatatgcgg tatttgattg gaaatggtga gatcaaaaca
     5641 aatgaactcg gtcgcatgga tggatttctg gtcgatagaa atacattgta tccttattac
     5701 tatgtcaaga aaacaggaga gaaagagtat gttctaaata ttggacgaag ctactcggac
     5761 atggaaaaag tagggaccat tcgacttgac tcaacagaag acacattgga acctgtcgga
     5821 ttgttcatcg tcggcggtga ttttgtcaaa gacgggttac gctatcatga gccttatcgt
     5881 ctgaaattga ttgcaaaaaa ggcacagtcg caggaagaac cagtggttta tagtcgtagc
     5941 caaagaagaa gacattcata atgtgatgaa acgccgtgta tcagtggttg cgataaaaac
     6001 gtttattcgg aataaggggg atgtgacacg tcttttgtca cgttcctttt ttatataacg
     6061 agaaagataa gacgaacaca tcacttattc tttcctttca tttataaaca ttgtagaata
     6121 aagaaagtac aacgaagtat tgtgaaaaaa cgtaataaat aacgaggaaa atacaagtag
     6181 gaggtaagct gtgaaagaat ttttgaaaaa ttgggggatt ttgatccttg tattagcggc
     6241 tattttatta gctagagtgt acgtgtttac accagttact gtaaacgggc attcgatgga
     6301 ccctacatta agtgatggac aacgattgat ttcatctaag atctcaaact atgaacgaat
     6361 ggatattatt actacaaagg aaccggggga tgaagaacgg atgattgtta aacggatcat
     6421 cggtatgcct ggagatacag tcaagatgga aaatgaccag ttgaccatta atggcaaaaa
     6481 gtatgatgaa ccttatctgg atgagttcaa aaaagaattt tcagaagata agttacaagg
     6541 agaatatgct tatagctcag gattccaagc acaagcagaa agttcatcca cctttacgag
     6601 tgactttgaa tatactgtgc caaaaggaaa gtatcttgtt ctaggcgata accgcttgat
     6661 ttcaaaagac agccgaatgt ttgggttagt cgacaaagat atgatccaag gaaaagttgt
     6721 tttccgttat tggcctcttt cagaaattaa aatcatgtga tgaaaaaata aaaagaagct
     6781 gtgacatgtt ttttgtcaca gcttcttttt ccgaaaattc cttcttattt cttggagttg
     6841 ctcacttttt ttgcaatctc attttaagct ttttggctga tggataagaa aagcgaagcg
     6901 tttttgttca taaatagctt ttccccacga tggaaggtat gactagtagc agagacaaag
     6961 cccgtttttt ccaatcgatt attcaattct tcttggctaa agccattatg gactttcgga
     7021 tgactaattt gttcattctt gtcaaagtct acgatgatca attgaccgcc aggagctaaa
     7081 atttcataaa gttttgtaag aatattctcc gtatcaggaa tgtgtaaaag tacaagtgat
     7141 agaaggatga gattagcttg gacagcagga aattcgacag aagcatcagc gtggatcgtt
     7201 cggacatttt tcagatctgc tgcttggatc ttttcttctg ccattttcag catagtttct
     7261 gaagcatcgg caatgatcag ttctttgaat ctttccgcaa gcggtaaact aacaagccca
     7321 gttccgcctc cgtaatccag caggactttt tggtggctgt cgcgcggtag ataagtagtt
     7381 agttcttcgc gaataatctt ggctaatgct tgacggtcag gggaatcata atggttagcg
     7441 atggaatcaa aaatattttt tgtcatcaaa tagctcctta cgtaaaactt tgaaatttta
     7501 acttattata gcacgtgaaa agaaagaaag catgctatac tcaattcaaa aagggagaat
     7561 ctagaaagaa tatgccgata tcgtttgtaa aagatagaga ggaaaaagga aagtgtgtcc
     7621 gagaaatatt actagacttg cctgagtggt ttgggcttcc tgaatcaacg gaaaaatata
     7681 tcgaggaatc aagtaaacta cctttatggt gtgaaaaaag aaaagaagaa tatttaggct
     7741 ttatcactct ttcacagaca agtgaagata ctgccgaaat ctattctatt gtatgggaat
     7801 aaaaaaagac tatcatcacc aaggattagg aacaaaactt tttaaagaag cagaaggtta
     7861 cgcagcaaaa cattacaaat atttgcaagt gaagactgta gacgaggggc actactctat
     7921 atatgatcaa actatttgtt tctacgaatc attaggattt tcaaggttgg aagtttttcc
     7981 aaatttatgg gatgaatgga acccatgttt agttcttgtc aaaaaattgg aacaaaagtg
     8041 agcaatagat ttaatactaa tctgtgcttg atgaaaattc tcactgtaaa aaatgctgaa
     8101 aatcaagtat aataccatag gaacaaactg gaaggtgaaa aagagcatgg cacaactatt
     8161 ttttaagtat ggcgcaatga atagcggcaa gacgatcgag atcctaaaag tcgcacataa
     8221 ttatgaagaa caaaataagc cagttgtctt aatgacaagc ggtattgata caagagatgg
     8281 tttaggcgtt gtttcaagcc gaatcggact gaaacgtaat gcgatgccga tatttgaaga
     8341 aacagacgta tttgacgtaa ttcagaaaat ggaaagcaaa ccctattgtg tgctgatcga
     8401 tgaagcgcaa ttcttaaaaa agaaacatgt cattgctttt acgagaattg tggacgaatt
     8461 ggatattccg gttatggcat ttggtttgaa aaatgatttt cgaaacgagt tatttgaagg
     8521 atcaaaatat ttattgcttt atgcagacaa aatcgaagaa atgaaaacaa tctgttggtt
     8581 ctgccacaaa aaagcaatca tgaatttaca ttatattgat ggaaaccctg tttatgaagg
     8641 cgatcaagtg caaattggcg gaaatgaagc ttattacccc gtttgtcgaa aacattactt
     8701 tcacccatca atgattacag aaggagaata agcatgtacg atcaattaca agcaattgaa
     8761 gaccgttatg aagagcttgg agaactttta agtgacccag aagtgatttc tgatacaaag
     8821 cgttttatgc agctttcaaa agaagaagca aacacgcgcg aaacagtcga agtttatcgt
     8881 cgttataaag aagtagtaca aggaatcacc gatacagaag aactgcttgg tgaaaaactg
     8941 gatgatgaaa tggctgaact agcaaaagaa gaattatctg aattaaaaaa agaaaaagaa
     9001 gtcttagaag aaaaaatcaa gatcttgctt ttaccaaaag atccaaatga cgataaaaat
     9061 atcatcatgg aaatccgtgg agcagcaggc ggagacgaag cagcattatt cgctggcgat
     9121 cttttcaata tgtaccaaaa gtatgctgaa gcacaaggct ggaaaacaga agtgatggaa
     9181 gctaacgtta ccggaatcgg tggatataaa gaagtgatca tgatgatcac cggagacaac
     9241 gtatattcga aattaaaata tgaaagtggt gcgcaccgcg tccagcgtgt tccttcaact
     9301 gaatcgcaag ggcgtatcca tacttccacc gcaacagttg tcgtgatgcc agaagctgaa
     9361 gaagtagaga tcgatattga agacaaagat atccgtacag atatctatca tgcttcaggt
     9421 gctggcggac agcacgtcaa taaaacagca tctgctgtac gtttgactca cttaccaaca
     9481 gggatcgttg ttgctatgca agatgaacgt tctcaaatca agaaccgtga aaaagcaatg
     9541 aaaatcttgc gtgctcgtgt gtacgataag atccagcaag aagcacaaag cgagtatgat
     9601 gccaatcgaa aatcagcagt cggaactggg gatcgttcag aacggatccg gacgtataat
     9661 ttcccgcaaa atcgtgtgac tgatcatcgt atcggattaa ctatccaaaa attagatcag
     9721 attttagcag ggaaattaga tgagatcgtt gatgcattga tcatgtatga tcaaacatca
     9781 aaactggaag ctatgcaaaa tgggtaaaat gacctatcga gaagtcctgt cacgggcttc
     9841 ttctttttta gaggatcatg ggaaagaagc atatagtatc caatttttat ttttggaacg
     9901 aaaacaatgg aaaaaacttg attggctcct tcatatgaac gaagagattt cagaagaaga
     9961 acagcggttg atcgaaaccg atctgcgact tctgcttgca gatcatccac ctcagtattt
    10021 gctaggatat gcagattttt atgatcatcg gttaaaggta actgaagcaa cactgatccc
    10081 tcgaccagaa acagaggagt tagtcgaatg gtgtttagac gagacgcctg gtgtaccgtt
    10141 agaagtgata gatattggga cagggactgg agcaattgct attagtttga aagcagcaag
    10201 aaaaaattgg catatatctg ctgtggatct ttcagaagaa gcattagaag tagcgaaaga
    10261 aaatgctcag aaagaaggaa cgaaaatcag tttttatcat ggagatacat taggaccagt
    10321 aatggatcag caatttgatg tgattatttc taatccgccg tacatcagtc ggaatgaatg
    10381 ggaactgatg gatgaaagtg tccggtcatt cgaacctaaa atggcccttt ttgctgagaa
    10441 cgatggatta gctatttatg aaaaaatagc agttgaagca ccaagcgtac taaaatcaaa
    10501 tggaaagatt tttttagaaa tcggatttcg ccaaggtgaa gcagtaaaaa aaatatttca
    10561 gcaagcattt ccagataaaa aagtggcgat aaaaa
//
LOCUS       DAXLPI010000088        10495 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXLPI010000088 DAXLPI010000000
VERSION     DAXLPI010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.088"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(1..459)
                     /locus_tag="ACF3T5_002012"
                     /pseudo
     CDS             complement(1..459)
                     /locus_tag="ACF3T5_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
     gene            complement(711..1070)
                     /gene="rplT"
                     /locus_tag="ACF3T5_002013"
     CDS             complement(711..1070)
                     /gene="rplT"
                     /locus_tag="ACF3T5_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFD3632586.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1123..1323)
                     /gene="rpmI"
                     /locus_tag="ACF3T5_002014"
     CDS             complement(1123..1323)
                     /gene="rpmI"
                     /locus_tag="ACF3T5_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFD3632587.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1395..1919)
                     /gene="infC"
                     /locus_tag="ACF3T5_002015"
     CDS             complement(1395..1919)
                     /gene="infC"
                     /locus_tag="ACF3T5_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFD3632588.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1963..2088)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2239..2871
                     /locus_tag="ACF3T5_002016"
     CDS             2239..2871
                     /locus_tag="ACF3T5_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFD3632589.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2873..3778)
                     /locus_tag="ACF3T5_002017"
     CDS             complement(2873..3778)
                     /locus_tag="ACF3T5_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFD3632590.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3863..4522)
                     /locus_tag="ACF3T5_002018"
     CDS             complement(3863..4522)
                     /locus_tag="ACF3T5_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFD3632591.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4838..5782
                     /gene="mvk"
                     /locus_tag="ACF3T5_002019"
     CDS             4838..5782
                     /gene="mvk"
                     /locus_tag="ACF3T5_002019"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFD3632592.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5775..6752
                     /gene="mvaD"
                     /locus_tag="ACF3T5_002020"
     CDS             5775..6752
                     /gene="mvaD"
                     /locus_tag="ACF3T5_002020"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFD3632593.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6765..7850
                     /locus_tag="ACF3T5_002021"
     CDS             6765..7850
                     /locus_tag="ACF3T5_002021"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFD3632594.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7847..8911
                     /gene="fni"
                     /locus_tag="ACF3T5_002022"
     CDS             7847..8911
                     /gene="fni"
                     /locus_tag="ACF3T5_002022"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFD3632595.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9160..9963)
                     /locus_tag="ACF3T5_002023"
     CDS             complement(9160..9963)
                     /locus_tag="ACF3T5_002023"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFD3632596.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9983..10306)
                     /locus_tag="ACF3T5_002024"
                     /pseudo
     CDS             complement(9983..10306)
                     /locus_tag="ACF3T5_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
     gene            complement(10339..10476)
                     /locus_tag="ACF3T5_002025"
                     /pseudo
     CDS             complement(10339..10476)
                     /locus_tag="ACF3T5_002025"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         3105 a         2220 c         2008 g         3162 t
ORIGIN      
        1 atacctaatg acatcaagat cggcaacaca ataactgcaa tagaaatcac tggtccgatt
       61 cctgtcatcg acatgaatat aacagcggtt acaatacata gtaagctcat cgtgattctt
      121 ggcttgtccc ctccaagttc aactaccttt cgaatcaggg tagctgcgat tccgctatct
      181 actagaacac gcccgaaaaa ggcaccaaag aagacattta ccaagatcga tttggcgtac
      241 tcagctggcc ccgtcgtata gatatgcgtc aacacatcaa ttactccttg cccttccatc
      301 gccgagtttg gtgaaaacgt gtttccgact agcgctaatc ccatccatac agtagccatg
      361 atgacaaaag ccgaccatca agttgtagcc ttttacacag taccagacca tgaaaaagaa
      421 tgagataact aacaaaatac caataataat atcactcatg ttattcctcc gtccattttg
      481 tttttctgat aagcaaacta atatatcagt caaactcatt ctaacttatg tagttagcgc
      541 tttcattaaa ggatgaccat aaatgttcag ttttcaatta aaaaacctta attatgttat
      601 tttagactta tacgttattg tcctgtttca tttgatggag gattgtaaat aaaaaaagag
      661 gaaagaacat aacttttcgt tatcttcttt cctctttata attgtttagc ttatttagcc
      721 aaagcttctt ttgcttggtc agccaatgca gtaaatgctg ctgcatcgtt aacagccaag
      781 tcagctaaca ttttacggtt gatgtcgatt tcagcaactt tcaaaccgtg cattaatttt
      841 gagtagctca agccattcat acgagctgct gcgttgatac gagcaatcca caatttacgg
      901 aagtcgcgtt tcttttgacg acgatcgcga tatgcgtagt tgtatgaatt cattacttgt
      961 tcttttgctg ttttaaataa agtgtgtttt gaaccgtagt aaccttttgc taattttagc
     1021 actcttttac gacgtttacg agttactgtt ccacctttaa cacgtgccat gtataattcc
     1081 tcctaataaa aataaatctt cagattctgt tatttgtact tcttatttca tttttgctag
     1141 ttgttggcga atacgtttga agtcgccact agaaaccatg cttgctttac gcaattgacg
     1201 gcgttgtttt tttgttttgc cgtggaaacg gtgacttgta aacgcgcgga agcgtttcaa
     1261 tccgccttta ccagtacgtt tgaaacgttt tgctgatccg cggtgtgttt tttgttttgg
     1321 catgactata tttcctcctc aaaatctttc gttatccgac tatcggctgt ccaacagcca
     1381 gttagtcatt caaattactt actgtcgttt ttcggtgcca gcgtcaagaa catgctgcgt
     1441 ccgtccattt tcgctttttg ttccactgtt gcaatatccg cagtttcttc agctaagcga
     1501 ttcaagactt tttgaccaat ttctttatgg gtaatggcac ggcctttgaa acggatagaa
     1561 gctttcactt tatcgccctt ttccaagaac ttacgtgcat tacgaagttt tgtattgaag
     1621 tcattcaagt cgatcgtagg acttaagcga acttctttta cattgatcac tttttgtttt
     1681 ttacgcgctt cgcggtcttt cttttgttgt tcgaaacggt atttaccgta gtccatgatt
     1741 cgcgcaactg gtggtttcgc accaggggca actaacacaa gatccaagtt tgcttgttct
     1801 gcaatattca atgcttctgc ttttgtctta actcctaatt gctcaccatc tgaaccgatc
     1861 aatcgtaact ctcgtgcacg aatgccgtcg ttaaccatca tatcctttgc tatggtcatt
     1921 cacctccaaa tattatgaga gaaagaaaag cttaccgaat aaacggcaat aaaaaaacga
     1981 gttctcttac agaacccgcg cgtacttcac ctaatagaac cagtgaaaac tatctagccc
     2041 agtgacatta tcaactaagg cgagaagcgg gtgcttctgc ttttatttct caaccattat
     2101 agtatagcaa aaactaatct ttttgtcaat gtttttcata cagtttgtct tccttttctg
     2161 aaaatgatta gattagcagg ctttctagca gtgattcccg cttctttgct atactaaaga
     2221 aaaacgaggt gaacccacat gaaaaaaatc gaacctattc caccttctct tcctcttctt
     2281 acaaagggag agttttattt agaagatgag gagatcttct ctggtcttct gatcaacgga
     2341 ggtaaccaaa gctatctcga tgccaaaaat atggtattaa gagagagtca tatccaaaaa
     2401 cttacgatgc aaaaaacagg tttacgtcat tttgaatgca gtaatgtcat ttttgaaaaa
     2461 tgtgattttt ccaatctgga gtggattggt ggcagtttcc accaagtcgt gtttcatcaa
     2521 tgtaaattaa caggtacgaa ttttgccgaa agttatcttc gagactgtac tttcactgat
     2581 tgtattgctt cgatggcttc ctttagtagt acaaacttga aagctgtctc cttcgatcat
     2641 tgtcaattag aagacagcga attttatgag gtcacttgga aaaacctttt tttgtctgac
     2701 aatcagttaa ccggttcgaa ctggtttcga acttctctga agggattaga ctttacgact
     2761 aatttcttca caacaatcgc tttatctcaa gattctttgg cgggactaat cgtcaatcaa
     2821 gagcaagcac ttgtgatcgc ccaagccctt ggtttgcaga tcaaagacta gatcaacgaa
     2881 tccgccgcca cagtgcaata agcatccaaa tagataagac caaactgatg atcaaggcga
     2941 tttcccatcc gaaaacggaa tgggtaaagg gtaactggag attcattccg aagaatcctg
     3001 tcacaatagt tggaacggtc aagatcagcg accagaccgt caaaaatttc atcgtgtcat
     3061 tcaagttgtt attcagcaaa ttattataag tgccagagag ctggtctaaa atctgcgaag
     3121 ctaagttcgt catttcaact gcttgtcgtg cttcgatcag tgcatcgtcc aactcttctt
     3181 tttccttttc tgacaacttg cgataaatag ccagtgcctt gatttgttct aaaagtacta
     3241 cattttgttt tgttcctgta actaagtaaa ccaaaccgat ctctaaatct gaaagttgca
     3301 gcagattctt gttcgttgtt ttttctctta atttcagatt aagccgctga cgttcactat
     3361 tcacttcctc gattaaagga aaaaaggaat cagaaatcaa aaacaatgta cgaaacaata
     3421 aactgtatac actcatgtct ggcatccgtt ccaaaagcga tcgaattagt cgaatcacgt
     3481 actcggtatt cggcgtggta aatgtaaaca aaccgtcttc ttttaaaata aaagcgatag
     3541 gactagtttc ataatgatta tcgacttttt gctggtgcgg aacatttgat acaagcagta
     3601 acgcttcatc aaaagaatcg tactctacgc gcgcccgttc atttttatct agagaataag
     3661 tcagcatctc gtcactaatg ccatattctt cttttaagtt tttaacagct agcacatcgt
     3721 tttgctgtac attgatccaa aaattacggt cttgattaaa atagtgtttt tcccgcatac
     3781 tctatcctcc tgacatttgt ttccagtata aagaaaaaag tcgctcaaat aaaggtttac
     3841 cctttatttg agcaactgta aatcaaatgg atactttggt ttggcgaatc tctgctaatt
     3901 tttcttcgat cattttcaag acttcttctt ttgcttgagg atcttccaca aaattatagc
     3961 ggtctccatc gatttggatt tttggacttt ctttgtattc ttcataccat tgatcataac
     4021 gtctgttcaa ttctttgtaa taatcgtata gacttgggtc aaaggacaat tgttcatacg
     4081 aacgaccacg tttttcgata cgctctaaca tcgtatcaaa agatacacga atatgaacta
     4141 ataaatccgg atgtttttta tgggcagcat agggaagttc ttccatcatg ttttccagca
     4201 gttcatcata cacacaaact tctgtttctg ttgctcttcc tagatctgca tttagatgga
     4261 aaagcagtga atcttcataa atcgaacggt ccaatacatt attgtcatct ttcattgctt
     4321 gtttgatgct tgcaaaacgt ttattcaaaa aataaatttg cagcaaaaaa gcatactgtt
     4381 cagggtttgc ataaaataag ggcaagactt cattatcatc aatcgattca taaaatgcct
     4441 gactatcaaa gtgttcagcc atcatttctg ttaaacttga ttttccggca ccaattgtac
     4501 ctgctaaaac gatcacgctc atttacctcc agctcacttc atcactaaat attgtgttta
     4561 actccaacct tttttaaata tacccccaat atctagtagt gtcaattgta ttttttctag
     4621 tcaaagaaga aaaaaagtca aaaaattctt aagaaaaaag tgttttttct ttagaaaacg
     4681 tattcttagt tttacttctg agatggtttt agtaaaataa aggaggaatg ttttttgaga
     4741 cccttagtgg acaaaaaaat tcgtgaagca gcgcaactcg ttttgggcgc tttatttttt
     4801 gcttaaaaat cacttttacg tgagaaggag ttagattatg gcaaactatg gccaaggaga
     4861 atcaagcgga aagatcatat tgatgggcga gcacgcggtt gtttatggag aaccagcgat
     4921 tgcctttcct ttctatgcaa caaaagtcac cgcattcctt gaagagctgg atgcaatgga
     4981 cgatcaactg gtttcttcct actattcagg aaatttagcc gaagctcctc atgcattaaa
     5041 aaatatcaaa aaattattca ttcacttaaa aaaacagcat gacatccaaa aaaacttgca
     5101 actgaccatt gaaagcacga ttcctgctga acgtggaatg ggatcaagcg ctgcagtcgc
     5161 cacagcagtc actcgtgctt tttatgatta cttagcattt cctttgtctc gtgaaatact
     5221 attagaaaat gtccagcttt cggaaaaaat cgcccacggt aatcctagtg gaatcgatgc
     5281 agccgctact agcagcttgc agccgattta ttttacaaaa gggcatcctt ttgactactt
     5341 ttctttgaac atcgatgctt ttttgattgt cgctgataca ggaatcaaag gacaaacaag
     5401 agaagccgtc aaagatgtcg ctcacctctt tgaaacccag cctcatgaaa ctggacaaat
     5461 gatccaaaaa ttaggatact tgacgaagca agcaaaacaa gcaatcattg agaattcacc
     5521 agaaacgttg gcacagacaa tggatgaatc acagtcactt ctggaaaagc tgacaatcag
     5581 caatgatttt cttaatctat tgatccaaac agcaaaagat accggggcat tgggtgccaa
     5641 attaactggc ggtggacgcg gtggctgtat gattgcatta gcacaaacaa aaacaaaagc
     5701 ccaagaaatc agtcaagcac ttgaagatgc gggtgctgct gaaacttgga tacaaggatt
     5761 aggagtacat acctatgttt aaaggcaaag cacgcgcata taccaacatt gctctaatca
     5821 aatattgggg taagaaaaat gaagaactca tccttccaat gaacaacagc ctttcgttaa
     5881 cattggatgc attttataca gagacagaag tcatcttttc tgatagctat atggtagatg
     5941 aattttatct agatggcacc ttacaagacg aaaaagcaac aaaaaaagtc agtcagtttc
     6001 ttgacctttt tcgtaaagaa gccggcctct cactaaaagc ttcagtaatc agtcagaatt
     6061 tcgttcctac cgcagctgga ttagcctcct ctgccagcgg gctagctgct ttagcaggag
     6121 cttgcaatac tgctcttaag cttggattag acgatctctc tctttcaaga tttgctcgac
     6181 gcgggtctgg ttcagcttgc cgaagtattt tcggtggttt cgttgaatgg gaaaaaggcc
     6241 atgacgactt aagttcttac gctaagccag tcccttccga ttctttcgaa gacgatttag
     6301 caatggtttt cgttttgatc aacgaccaga aaaaagaagt gtccagcaga aatgggatgc
     6361 gtcgaacagt cgaaacatcc agtttttatc aaggctggtt agattccgtt gaaggggatc
     6421 tatatcaatt gaaacaagca atcaaaacaa aagatttcca acttctcgga gaaacgatgg
     6481 aaagaaacgg actaaaaatg catggaacga ctttagctgc ccagcctcca ttcacttact
     6541 ggtctcctga cagcttaaaa gcgatggatg cggtgcggca attgagaaaa aaaggcattc
     6601 catgctactt tacgatggat gctggcccta atgtcaaagt tttagtggaa aacagccatt
     6661 tatctgaggt acaagaaaca tttaccaaat tatttagcaa agaacaagta attactgccc
     6721 atgctggtcc aggaattgcg attattgaat aaagggagat ttttatgatt gaagtatctg
     6781 caccaggcaa actctatatt gccggagaat atgcagttgt tgaaacagga catccagcag
     6841 ttatcgctgc agtcgatcaa ttcgtaacag tcactgtaga atccgcacga aaagtcggaa
     6901 gtatccaatc tgcacaatat agtgggatgc ctgtacgttg gacaagacgc aacggagaat
     6961 tagttttaga tattcgggaa aatccttttc attatatcct tgctgctatt cgtttgactg
     7021 aaaagtatgc acaagaaaaa aacattcttt tatcctttta tgatctgaaa gtaacaagtg
     7081 agttagacag ttcaaatggc cggaaatatg gattgggttc tagcggtgcc gtgaccgttg
     7141 caacggtcaa agccttgaat gttttttacg cgttgaattt atctcaattg gagattttca
     7201 agattgcagc actagccaat ttagcagttc aagataatgg ttcctgcggc gacatcgctg
     7261 ctagctgtta tggtggctgg atcgctttct caaccttcga ccatccttgg ctccaagaac
     7321 aagaaactca gcattctatc agtgagttac ttgccctgga ttggccaggt ctatccattg
     7381 agccattgat tgctcctgaa gatttacgtt tattgattgg ttggacgggt agccctgcct
     7441 ctacttctga tttggtcgat caagttcacc gttcgagaga agacaaaatg gtggcttatc
     7501 agcttttctt aaaaaacagt acagaatgtg tcaacgaaat gatcaaaggg tttaaagaaa
     7561 ataatgtaac gttgatccaa cagatgattc gaaaaaaccg acaattactg catgatttat
     7621 ctgcaatcac tggggtcgtc atcgaaacgc ctgctttgaa caaattgtgt aatttagctg
     7681 aacagtatga aggagccgca aaatcttctg gtgcaggtgg tggcgattgc ggaatcgtaa
     7741 ttgttgacca gaaatctggc atccttcctt taatgagtgc atgggaaaaa gcagaaatca
     7801 ctccactgcc gttacatgtc tatagcgatc aaagaaagga aaaccgatga atcgaaaaga
     7861 cgaacatgtt tcactagcta aagcctttca tgataaacaa aaaaacgaat ttgacttcgt
     7921 acggatcatc cataaccctt tgccgcaaat agcagtttct gatgtcgatc taagtacaca
     7981 ggcagttggg tttacactaa gcagcccatt ttatatcaat gcgatgactg gcggaagcga
     8041 aaaaacaaaa aaaatcaacc aagatcttgc aatcgtcgca agagaagcag atttgatgat
     8101 tgccactggt tcagtcagcg ctgccttgaa agacccttct ttagccgata cttatacgat
     8161 tatgcgacaa gaatatcctc acgggaaaat cattgccaac atcggtgcag ggacttctgt
     8221 cgaaagagcc caagaagcta tccgattgtt tcacgcagat gccttacaga ttcatttgaa
     8281 tgcacctcaa gagttggtta tgcctgaggg agaccgtgat tttaccaatt ggaaagtact
     8341 tattcaagag actcaaacag ccattgacgt ccccctcatc gtaaaagaag tcggttttgg
     8401 catgacaaga gaaacgctca atgatctagc tgctttagga gttcatacag ttgatatcag
     8461 cggtcgaagc ggaacaagct tcacccagat cgaaaacgcc cgtcgctcca agcgggaact
     8521 gagttattta gctgactggg ggcaatcgac agtctcttca ttgcttgaag caaatgaagc
     8581 agacacttct atggaaatcc tagcctccgg aggtatccgc aatgcctatg acatatttaa
     8641 agccctttgt ctcggcgcaa atgcagtggg tacttcagga accgtactga cgcatctgat
     8701 gaatcacggc gtagaagaaa ctattatact gatgaagcaa tggcaagaag agcttcgtct
     8761 gctttatact atggtcggcg caacgaatac cgcggcccta caccaacaat cactgatctt
     8821 ttccggacca gtgaaggatt ggtgcgaagc aagagggatc gacttagtga agtatggaag
     8881 acgcacagaa aagagcgtgg gacaaaagta aagaacactt ttatcccacg ctctaaacac
     8941 gtataaacgg cggaagcaaa agcaactcct tcggaaataa gctgaaattc acaaaaattt
     9001 gaaaagcaat tttcgtgaat tccttcttat ttcttggagt taaacgcttc tgtcccagcc
     9061 tcttttttcg aataaatgat gtttaatcag ctgtccttcg gtattcgttc aagccgctga
     9121 tttcacaacc tctccactaa acggtgtact aaagctcttt taaggttgtt ttccgatata
     9181 ggcaagtatg cctccgtcca catacaagac atggccgtta acgaaatcag atgcgctaga
     9241 agccaagaag acagatggtc cagccaaatc gattggctcg ccccagcgtg ctgctggcgt
     9301 acgtccaacg ataaattgat cgaatggatg acgttcaccg ttttcttgtg tttcgcgtaa
     9361 tggagcagtt tgcggtgtag caatataacc tggtccaatt ccattacatt ggatattgta
     9421 ttgaccgtat tcagaagcga tgtttttcgt cagcattttt aatccgcctt ttgccgcagc
     9481 ataagcactg accgtttcgc gtcctaattc actcatcatg gagcagatat tgatgatttt
     9541 accgccgcct ttttcgatca tatcccgaat aactgctttt gaaacgatga atggggcatt
     9601 taaatccacg tcgattactt ggcggaagtc tgccgcatcc atttcgatca tcggtgtccg
     9661 tttgatgatc cctgcattgt tgactagaat atcaactgaa cctacatctt ctttgatctg
     9721 ttgaaccatc gcttgaaccg cttcttcatc ggtcacatcg cagacataac cttttgcatc
     9781 gatgcctgct tctttataat gcgctatacc ttcatcaact gattcttgat tcaagttatt
     9841 aaatacgatt tttgctcctg ctgtagctaa agagcgtgca atctcaaatc caataccata
     9901 gacagcaccg gtgactaatg ctacttttcc atctaaacga aacatatcca tactaaattc
     9961 catcattttc tctcccttat gcttatttta attgatccat ttgcaccata tccatgtctg
    10021 tataggtgat attttctccg cacattgccc agatgaatga gtaattgctt gtacctacac
    10081 ctgaatggat cgaccagctt ggcgaaataa tggcttgttc attgtctacg actaaatgtt
    10141 ttgtttcatc tggtttgccc atcatatgga acacacgtgt atcttcattg gcataatcaa
    10201 aatagacata agcttccata cgacgttcat gtgtatgaca aggcatcgta ttccaagcac
    10261 ttcctggttc taaaatcgta taacccattt ataaatgaca gctatcgctg aagcttctac
    10321 tgctgtaaat atccccgtca agataccacc gataatgatg acaatcaata gaaggctagg
    10381 aatcgcctct tttattattt ttccagcatg agccatctgt cctttttcga caacagggta
    10441 tttattcttt ttagcaacga tgaatgctac tagcatgatt cctagcgccc acaaa
//
LOCUS       DAXLPI010000089        10494 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXLPI010000089 DAXLPI010000000
VERSION     DAXLPI010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.089"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            132..725
                     /locus_tag="ACF3T5_002026"
     CDS             132..725
                     /locus_tag="ACF3T5_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632597.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            747..2441
                     /locus_tag="ACF3T5_002027"
     CDS             747..2441
                     /locus_tag="ACF3T5_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFD3632598.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            3129..5006
                     /gene="ltrA"
                     /locus_tag="ACF3T5_002028"
     CDS             3129..5006
                     /gene="ltrA"
                     /locus_tag="ACF3T5_002028"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFD3632599.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            5137..5514
                     /locus_tag="ACF3T5_002029"
                     /pseudo
     CDS             5137..5514
                     /locus_tag="ACF3T5_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            5514..6719
                     /locus_tag="ACF3T5_002030"
     CDS             5514..6719
                     /locus_tag="ACF3T5_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFD3632600.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            6735..7379
                     /locus_tag="ACF3T5_002031"
     CDS             6735..7379
                     /locus_tag="ACF3T5_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632601.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            7390..8196
                     /locus_tag="ACF3T5_002032"
     CDS             7390..8196
                     /locus_tag="ACF3T5_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632602.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            8218..8448
                     /locus_tag="ACF3T5_002033"
     CDS             8218..8448
                     /locus_tag="ACF3T5_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632603.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            9005..9280
                     /locus_tag="ACF3T5_002034"
     CDS             9005..9280
                     /locus_tag="ACF3T5_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632604.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
     gene            9322..9744
                     /locus_tag="ACF3T5_002035"
     CDS             9322..9744
                     /locus_tag="ACF3T5_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632605.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            9764..>10494
                     /gene="mobL"
                     /locus_tag="ACF3T5_002036"
     CDS             9764..>10494
                     /gene="mobL"
                     /locus_tag="ACF3T5_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HFD3632606.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNT"
BASE COUNT         3921 a         1662 c         2211 g         2700 t
ORIGIN      
        1 gaccctataa taaccctggt caacgccaaa tttttgcgat tatcgaatct attttcttta
       61 cggataagaa ccactatcat gcgttagatc ctaaccgaaa ttatcatggt acgtataagg
      121 aggatgaata aatgcgagtc aaaggacaaa attcagttga taagaaatcc attttgcagt
      181 ttggaaaacc agaagagcgc tctatcttgg acttatttcc atataaatcc tgtacagaag
      241 agggagtctt aattactaga gaagatcatt ttcagcgtta ttatcgtgtc gtttccactg
      301 acgtggaggg attaaatgaa caagagaaac tagaacgaat gaatcagttg accaccgtca
      361 tgcgtacgta tgtgccaaca attaagttag ttagtttgac cacagagacg gatctaacgg
      421 aacaaatcgt gcaaaagcgg cagttattag ataaaaatcg tatggaacag tcactaggaa
      481 gaaatctaca gcgattaaga aaatatgaaa agaaattagt agaggagatc caggaattaa
      541 aacgggcaga acaagaacgt cctgacttaa gcttcttttt cgttattgaa ggaaagacaa
      601 tcaaggattt acaaactaga aatcgtcagt taatgcgatc tagtggcatt ttagggttaa
      661 aaccactcta caaaaaagaa ttgattaaga ttctttatcg aatgaacaac atgaatgacg
      721 aataaaaaag atggaggaaa tgattcatgc tagatgtgat aaaaacagct ttttcaacgc
      781 aaagtaatca aaaggtgatt gatccattaa aacaacttgg taatcgtgaa atagagattt
      841 taaaatcaaa aggttatgat ttagatttct taaaacaagt gatgccacaa ggtgggttga
      901 attttgatga agaatatgtg acctatggaa atggttattc acgatgtgtg acagtttatc
      961 attacacaaa aaatcctact cttttttggt tggccaatct catgaacaat cctcatacag
     1021 tggccacact agatgttagc acagatgaaa agcaaaaagt gattgatcaa atcaaccgcg
     1081 cattagatga attaaatgat cgatcagata atgaacgaaa agcgactgac cgaaataaaa
     1141 gtgcggaaga atacctggaa ctagcggaat atgcccgaaa attgacttct aatggggaaa
     1201 tttctaaaca agtcaaagtg agagtacatg tttacggaaa tacgatccaa gaagtggatg
     1261 aacgggcgca ggaactgatc gaaaatctaa aagggatgga ttatcgtgcg gtgacttatc
     1321 tgcataaaac caaacgagat tggcaatcga tgtttaccat gttcgatgaa caagctgaat
     1381 ggtttggaaa tatcaaaggg caatcacttc catcgttaaa cattggcgga ggattcccgt
     1441 tccaccacca aacgctaaaa gatccaggag gcacacaata tggggaaacc atgacaggcg
     1501 gcccatttgt ctggaatgcc acgtataaaa atagtgtacg tttatctacc aataccttgc
     1561 ttctaggaat gatgggagct ggtaaatcga ccgttcttaa aatgatcgcg gaagcccatt
     1621 tagctgcagg agactttatt tggggctttg aaaagggaaa agactttatt cctttcttaa
     1681 aggaatataa cggtattatg gtgcgtttag acggatctga cggaatgatc aatcctttag
     1741 agatatttgc cacacggacg tatgatgaag catccagttt atatgacgat ggatcagtca
     1801 aagatttgaa gatcaatgaa gcagcgtcct atcaaacaca cttagacaaa gtggtctatc
     1861 aggtacagtt agtgagtcct cagctaaaag gaacgatgaa agcagaattt aagacctact
     1921 taaatcgttt ttatgaagag tatggaacag taccacgcgg ctttacttcc tcaaattcgc
     1981 gttcaaatac tgatacgcaa gtcacaggga aagatccaga agcttatccg acgttcaaag
     2041 aatttttagc ctttttagga caactggaac taccaggggc ttcccaagag aaaaagaatc
     2101 ggaaagaaga aatggagtct atcgtggaat ccttatgtga gacttatggc atgatttttg
     2161 atggtcatag tacgattcga caccttgacc aacaacagtt ggtctttttt gacattgact
     2221 ctattacagg cctagatcga ggggtacaac aatgtcagat gtacatggcc atgacgttga
     2281 tttggaatca ggcattgatt caaggacgaa gacaacgacg cttaattaaa gaaaaacaaa
     2341 tggaagaaca agacttcaaa cgcttcttgg cgatgattga tgagtgccat aatggtgcga
     2401 cctgttctgc agtgaaaagc tgtgggttgc aagtagtcta aaaactattg taataactct
     2461 tataacgaga agtcaaatga gtgggtaacg ccacaaaggg cttccactga tactccgaca
     2521 tgcaacaagt agtttatgca gaccaacgtt gtgtgaagct cggtgaagtc ggctgaagga
     2581 taccgtaagg taaaagaaat agtgaatacc acgaaagttc tctagaggta aagggtgtat
     2641 ccgataagtc gggtgtccaa aaaaacttaa tatggtgagt atgtacaaac gtactgacga
     2701 actagcgaat gtactgtccg aaaggcatag ctcctagaaa tgggagagac agtgagcaaa
     2761 aaatcactca ttgtgctaac ttgtggttga gtaaaagtcg ttgttatgaa agaccatgct
     2821 gcgttacagg cgtaggagaa gttagggggc aaaagctagc atctaagtta aggcatgtgg
     2881 atagatataa gggaacaggg gaagccatca acatggagag tgcatactcg atgaagtggt
     2941 agtaagaaat atgtgaatga caaataactt actttagtga tggtgaaagt agagtcacga
     3001 ctgatgaagg ttctgtaatg gaactggagg aacaggctcg attcgtctgt aaaagtcatg
     3061 aaacccgttc aaccatggga ttctcgtatg actaaaaagg tcacgagcca attggagcgt
     3121 gataaaccat gacaaaagaa acaagtccaa aagataggaa gttgcggcat gcagaatatt
     3181 atggcatgac ggaaatcttc gataagatat actccaaaag tttgaaggga gataatttca
     3241 agaatctaat gcagattatc atttctgata acaacattgt cctagcatat cgtaatatca
     3301 agagaaatca cggtagtgtg acaagaggag aagatggagt aacgattaaa gatattgaaa
     3361 atctcaaccg taatgaattc gtagaaattg ttaggaagcg attcgcacat tataaccctc
     3421 ggaaagtaag gcgtgtagaa attcctaaat ataatggtaa attaagaccg ttgggtattc
     3481 catcaatatg ggatagaatc gcgcagcaat gcatcttaca agtattagaa ccaatatgtg
     3541 aagcaaaatt caataatcac agctatggtt tccgcccaaa tagatcagca gaacacgcaa
     3601 tagcagattg tatggcccga atgaatcaaa gtcacatgga atatgtggta gatgtagata
     3661 tcaaaggttt cttcgacgaa gtcaatcata ctaaactcat gagacaaatc tggtctatgg
     3721 gtatacgaga caaacagctt ctcttaatca ttaggaaaat gttaagagca cccgtagtac
     3781 ttccaaatgg gaagttgata tacccaacaa aaggaacacc acaaggcgga atacttagcc
     3841 cattacttgc gaacatcaat ctcaacgaat ttgactggtg gatagcaaat cagtgggagg
     3901 aaaaagatgc caaagagcta aaacatcatt ttaggaaaaa tggagcttgg gataaaggta
     3961 atatctatca caagctcaga aagagtacaa ctctaaaaga aatataccta gtgagatacg
     4021 cagatgattt taaaattttc tgtagaaatt ataatgaagc gaaaaaagtt ttctatgcaa
     4081 ctaaaatgtg gttacaggag cgtctaaagt taccaatatc tgaagaaaag tcaaaaatca
     4141 caaatctttc aaaagaagaa agtgaatttt taggctttac gatgaaatta gtgaccaaag
     4201 gtaacaaaaa ggttgttaat acgcatatag gtacaaaagc acaaagcatg attaaagtga
     4261 aactaaaaaa acaaatcaaa cgaattcaaa aaagtcctaa tagcaaagat acctttatct
     4321 atattggagt ctataacagt atggttattg ggattcataa ttacttcaaa attgcgactc
     4381 attgtagtgt agcaatgcga aaaattgcaa gagaactcag accaagtttt gaaatacgac
     4441 tacgagaaca aggaatctca aaagaaggac aatacggaaa gtacgaagga tacaagcctt
     4501 acctacggtc aggacaaata cgatatataa gagggtttcc aatattacca gtagggtata
     4561 ttcaacacag acatccgatg tgtaaaaaaa gaaatattaa taaatacacc gaagaaggta
     4621 gggaagccat tcataagaag caacaaaccg tagaagaatg gaagatatcg tggttgtgta
     4681 gcaatccagt gcttagtaag agagcaacga ttgaatttgc ggacaatcga atttcaaaat
     4741 tcatcgctca aaagggaaga tgtgcagtaa caggtgagga gcttatctta tctgaaatgc
     4801 attgtcatca tattatccct tatcatgaaa gcaaaagtga tagctatgaa aacctagtga
     4861 ttgttactga ggaggttcat cgggtaatac acgctacaca aagtgaaacg attgaggaac
     4921 ttctaaaata tctaaaatta aatccaaaac aaaaagaaaa actcaatgag ttgagattaa
     4981 aagttggtaa cgaagaaata tcttaataaa tatttgtagg acgggaaaac agaatgtctt
     5041 ttatagacga gggaacgccg tatgcggtga aagtcgcacg tacggtgtgg actgggggaa
     5101 aagacggaga taatatcaaa ctcttaccta tcagtatcga ttaatccaga ttttattgaa
     5161 acggtaaatt atgtcacaaa ctttcaacga gaaatgcgta aagtagaagc aatgactgtt
     5221 ttagctacac aatccccaca agaaatggtg ccagaaaata tgtcttctga taacttctcg
     5281 aaactgaaga aagtctttga attttcttct acgaagatct ttatgcgtat ggatgaatct
     5341 attttacgtc atatagaagg attagtgggt aaatcaatga cgaactcaga attagaatcc
     5401 attcctcaac aaaatcaagg agacgctgtg atcaattttg gctcaaaaga aaccattcgt
     5461 gttcatttta gtccgaacaa acgtcaatta aaattgtttg atggaggaaa ataatggaaa
     5521 accaaaatga aagtttaatc aagcagtatg ttaagcgtcg tgcaaagcgg cgcttatttt
     5581 tgtggttgtt tggtacttct gcaggtttga ttactatttt gatcactgtg ttcgtgaccc
     5641 tatttctaat acttgcggcg ggctcgattg ataattcgga ttcagattca agcagtggtg
     5701 gcgaagcttt tacaggagaa tattcggaag gcttaccgat ttataaagaa atcaaaggaa
     5761 gaggcccttt ttctgatgag attgcgcaat atgccgtagg tgcagcagtg aaatataaat
     5821 tattaccaag tgtgatcctt tcgcaatacg gttacgaatc agcgtttggt acatcggcgt
     5881 ccgcaagaaa tgatctcaac tattttggga ttacttggtt tgatggttgt ctgtttccta
     5941 aaggaactgc acgaggaatt ggtggcattg aaggtggctg gtacatgaag tttcccaact
     6001 caaaagctgc attttcctat tacggcttta tggtagccac acaatccaat tttaatgctt
     6061 gtgtaggaaa taaaagccct ggcgcaagct tattgatctt aggacgaggc ggttatgccg
     6121 ctgcaggaat tacggaagat agtccgtatt atacaggttg tatgtcaatt atcacttcta
     6181 ataaattaac cgaatatgat gaattcgcca tcaaacattg gggtgaaggt ggcaataaca
     6241 acggcacgat tactggtgaa tggacgaatc cattccctgg cagcagttta gataaaagtt
     6301 ccttcagcgg tggtcaatta tttggaacga atccaggcgg tgaattccgt ccgaacggct
     6361 tccatgatgg attagatttt ggttcagttg atcatccagg aagtgagatt catgcggttc
     6421 atggcggaaa agtcgtgtac gttggcaatc ctggtatttc aggtttgggc gcgtgtgtga
     6481 tcgtgattaa ttacgatggc ttgaatatgg tgtatcaaga atttgcaaat agtacaggaa
     6541 attctcgagt gaaagtaggc gatcaagtga aagttggtca agtaattggg atacgagata
     6601 cagcacactt gcatttagga tttactcgaa tggattggcg tcaagcacaa ggacatgcat
     6661 tcattgatga tggtacatgg atcgatccgt taccattttt gaatagcagt aaaaaataga
     6721 tcaggagaaa aaatatggaa aataaaacgt taaaaatcgt cttaacagct gcaattagcg
     6781 ttagtttaat cggcaatatt gcgttgtttt ggcagctgaa agaggaacaa gcagcaaata
     6841 cctataaaat tgaagaagtg gctaaaaacg cgtctgagaa gctcaaaaaa aattcctctg
     6901 aggcacaggc gaagatcgac aagttaaatc aaaccatcaa tgagaaaaac caagaaatcg
     6961 atcagctgaa gcaacaagat ggcacaaaag caaaagagga agtaactgaa gcacaaagaa
     7021 agacggcaga agagttcggt caattggcca ttgataaatc attaagcaga aatgaattaa
     7081 cggaaaaact aaaagatgtc gcaacccaag aagtgatcga caaattaagt cctacagacg
     7141 atgatcacca acatgatgat tacggaagtt actcctttac attaggtgac gtgcaaactt
     7201 acgctcaaac aagcagtgta aaagaggaac aaaattttgt cgtatttgtt gattatacaa
     7261 tcggcaatcc gcaattcaaa gatgtcaaac cacaaaaaat aaaaggtgga ttaactgtca
     7321 ctgaaaaaca agtagatggt caatggaaag tcatagactt tagttatttc actcgttagg
     7381 aggaagaaaa tgagtaagaa aaaaggagaa agacaacgtt tcaaaaaacg aaaaagttat
     7441 cttagatggc tgggaatctt tatgattctc ggctttttga ttcttgctag tccgatttat
     7501 acgaatcaga aggtagatat accagatgag gtaagcacga acgccttttt aaacacgaaa
     7561 gagctgacaa tggtttcaaa agaaaaagga gcgaaccagc aattagtttt tgaatttgcg
     7621 atcgatgatg aagatcaaag cttgttaaaa gaactggcta atcttaatta tcgcgtagaa
     7681 gtaaagacag ctaaagggga ttatcaagca atacgagcca aagtgattaa ggtgtctgat
     7741 gattattttg cagtgaagct aacgcatgta ccagaagaat atatcgccat gcgtttaacc
     7801 attattcctg aaaaaattga tccaaaagtg gatatgcaag aaccacaaga tctgatttat
     7861 tacgtgcacg aagataaggt taaagatcaa gtgaaagatg aagattatga acaacacgcg
     7921 atcaattaca aagtgaaagg atacaagaaa gaacaaaaag cagctcaaaa acaaatggaa
     7981 tctttccaag caaccattga tttgaatgaa gaattagtga agaagctaaa agatcaattg
     8041 ccttatcaag tcgcagatga tcaggaaaat actgaaaaca agattaatag ctaccagcaa
     8101 gaaattgaaa catcgaaaac gcaactgaaa gatcaagaag agatcattca aaaacttcaa
     8161 gaaaaaattg atcgtatgac aaaagaaaag atttagaagg acaggtggag tgaaaaaatg
     8221 aagcgaacaa acttggctga caaagatact ttcgctgagc agaaagtatt gattaaagcc
     8281 tatctcaaag ttgtaaaagc taaagaaata tgtcgagaag tgaatttatc taacacaaca
     8341 atatctgagt tgaagaatgg tcgaaagaat ttagatactt taaaattcag atcatttgaa
     8401 gctctatatg tagctgctaa aaaatatttt acagctgata acaaataaaa aacaggagta
     8461 ttttatgtga aaaaagtata taaaaaatgc ttatgaatca gctatatcac cttaaccatt
     8521 tgttaatgag atgggagaaa acattaacca cgattctaac gcaaacatca agcacataaa
     8581 acatttaaca ctcctccttt ctggctgaag atttcaacca aacaggaaga cagcgtatca
     8641 attatagcat acactttttc ttaaattcat ttttcatatg acaaaatatt tccttctaat
     8701 cgacaaagtt agaaggattt tttattgtct aaatacacaa ataaccttaa tttttaaaga
     8761 gcataaggaa acgtgtaccc actttcaaaa taaagtgggt agataggctg tgcctatcac
     8821 gtttctgaaa aaaataaatg accccaaaat tttcaattta tgtgtaggtt tatgggtagc
     8881 aaaaatatga atgtgggcag caaaaagttc atttgtgggt agtaaaattc aaatgtgtgt
     8941 agcaatgtgt gtagctacac acattgctac acacgctata acatatagtg aaaaggagat
     9001 atttatggaa gtaaaaataa gagatattga tgaaaaaacg attcaaagaa ttgaccgaag
     9061 atgtattgaa ttaagtgaac gcaccgggac taaatggagc cgaaatcgat acctaaaagt
     9121 tttgattgaa aatgattttg accatgaact tatggatcat agaaaagatc aatttgatcg
     9181 gttgttagaa aaatttgtgg atattcaaac ttataatacg aaaattttag aagaatatat
     9241 tgcaacaaat aatcaattaa tcgccttgtt aattgaataa taaaaatagt attgtgaatt
     9301 tataaaagga ggaaatcaat tttggcaaaa gcaacaagaa aacatgtata tctttacgat
     9361 atgcatatcg aagtacttga tgaaataatt tctactcatg aagatatatc taattatagt
     9421 gaagcagtca gacatttgtg tatgatgtat gatccaaaaa tcgaagaaaa aataaatcca
     9481 attcaaatta aattgaatgc aatgggaaaa gaaatttcta tattaaccga aatggtttca
     9541 gagtttgcgg atcttcattt gaaagataca ggaatactaa ttggtacaaa ttcaactgtc
     9601 tatcaagaag caaaagagcg tgtggaaggg aaaataaaag cgaatcaaac gaaaaagtat
     9661 tctcctaaga tcaagaaacc aataaatgaa tctgttcatg ccagagtaga cccgtttgat
     9721 ccaacaaagt ttttaaaaag ataaaaagta gaaggggaac atgatgaata aatcgcctgc
     9781 agtcattctc acttgtcaat ttaccttgcc caataaaaaa tctttttcaa catacattga
     9841 ttatatgaca agagaaaaag cgctggaaga aaaggaacga cgcacgccgg acgaagaaca
     9901 ggaactgcaa cgaatcaaga atgctttaga ggatttttat attcctgaag gagaaacttt
     9961 ttcggagagt aagacagata aagaattcag tgaaacgtcg aatgaagctc aatcgttgat
    10021 ggaacatgga atcaattttg acaagttaga ggaacaagat tttaccaaat atctttctta
    10081 tatgacccgt cactatgcgt tagaacaaaa gaaagaactg acgcaaaggg aacaaaaaga
    10141 ataccaacgg ttgaaccaag caatgaaaaa atatcaaaca gagccacata aaaaagcttc
    10201 aggaaatctt cctggagttt tttctatggc ttcagacgaa gtgaaaggaa gtgatctgaa
    10261 ggagatcaaa caaatcttcc aaagagggca acaaaaaggc tcaatcattt atcaagatgt
    10321 tgtctctcac gacaattcgt atcttgaaaa aatgggcttg tacgatccca aaacagatac
    10381 cttagatgaa gaaggattaa aagcagctgg aagaaaaatg atggagacat tattcgagaa
    10441 agaacaattg aatgaaacag gttactggat ggcaacgatt caccgtaaca ctaa
//
LOCUS       DAXLPI010000090        10414 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXLPI010000090 DAXLPI010000000
VERSION     DAXLPI010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.090"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            114..854
                     /locus_tag="ACF3T5_002037"
     CDS             114..854
                     /locus_tag="ACF3T5_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632607.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(1145..1813)
                     /locus_tag="ACF3T5_002038"
     CDS             complement(1145..1813)
                     /locus_tag="ACF3T5_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFD3632608.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            1947..2234
                     /locus_tag="ACF3T5_002039"
     CDS             1947..2234
                     /locus_tag="ACF3T5_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632609.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            2650..4188
                     /locus_tag="ACF3T5_002040"
     CDS             2650..4188
                     /locus_tag="ACF3T5_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFD3632610.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTSTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            4185..5150
                     /locus_tag="ACF3T5_002041"
     CDS             4185..5150
                     /locus_tag="ACF3T5_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632611.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            5137..6459
                     /locus_tag="ACF3T5_002042"
     CDS             5137..6459
                     /locus_tag="ACF3T5_002042"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFD3632612.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      6522..6626
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6700..7443
                     /locus_tag="ACF3T5_002043"
     CDS             6700..7443
                     /locus_tag="ACF3T5_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632613.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            7458..8450
                     /locus_tag="ACF3T5_002044"
     CDS             7458..8450
                     /locus_tag="ACF3T5_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3632614.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            8462..9199
                     /locus_tag="ACF3T5_002045"
     CDS             8462..9199
                     /locus_tag="ACF3T5_002045"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632615.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            9189..9911
                     /locus_tag="ACF3T5_002046"
     CDS             9189..9911
                     /locus_tag="ACF3T5_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFD3632616.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9995..>10414)
                     /locus_tag="ACF3T5_002047"
     CDS             complement(9995..>10414)
                     /locus_tag="ACF3T5_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFD3632617.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3458 a         1792 c         2171 g         2993 t
ORIGIN      
        1 tatatttatg aaattcatcg ctattttctt aaaataatca gcgcattgct actgaaaaca
       61 atgaatctgt gctattattg agaaaagttc catttttgag aaggatggtt tgaatgaaag
      121 aaatcagacg aaaacatttc cgttttcctg cgtacgatga tgagttaggc gtaaaattaa
      181 ccaacgacaa gaagcgagta ttatttgacg gacaagaaga tcttttgact gaacagaatc
      241 agtccccgtt tgaacagatg aaagaatttc atttcacgaa cacgaacacc gagaaaatcc
      301 gtcgcaagcg tcgtgcaaca aatggattga agtcagctcc gcgtaaggaa aatttggaaa
      361 agcataaagg aaaattaccg gattataccg gccattacca atcagaaatc acatcgacag
      421 gacgaaaaaa actttttggt gattcctctc gttctgtcag acaaagtgaa acaaggacag
      481 agtccaagca tactacacga aacgcatctg ttgacacgaa ggtgcgggag cgttcttatt
      541 ttgttcctaa atatgtaccc gcatcgatta tcccagaccc tagagaaagc aaaatttctg
      601 aggatcattt aatcgaatcg atgaaaaaaa cggatgactc ttatctgttg ttcgatacag
      661 aaccgacgcc gtatcaagaa aaaagaaatg gtgagccgtc tgtacgaaca ttcaatcgaa
      721 caaaaacgtt agaggaaaca aagcagtcag aagaaaaaaa atcgaaaaaa agccatggta
      781 tcttagaacg ctcattacaa ggattgatcg atgatcaagg aaactctttg ggagaaaata
      841 gctattttaa ataagtgata tacaaattta tattcggcta cagttggaaa ggaaacgctg
      901 cggcgtttcc ttattttata ctctatccga aagtttatat cgtatcctca aatttttttg
      961 agtttaagcg aattttttct cccaataagt aagaaaataa tggcaaaata aaaaaacaaa
     1021 ctattttcgc agatcgtttg caaaaatagt ttattttttt ataaagaaga ttttactgaa
     1081 taatatcatt ttagtttaca taatgatatt tctgtaaatt aaataaagta gtgaaaaata
     1141 agaatcagtg ctctaactgt tcaagaggat caagtggttc aaatgtttct gtagtctgtt
     1201 ttgcttccaa ttggggcaaa acagatccaa tttccagata cttcacttta ttcaatgtat
     1261 aagatagaat agaacagcta agtaatgtat acagaaaatg tgtgagatcc aaataaagca
     1321 aggagacaac taaaatacca aaagtaatca agaaaagact tttgccaatc ttaatatgaa
     1381 aatatttttc aatggcttga taaatgatat ctgttccgcc tgtgcttccg ccgaatcgaa
     1441 agacgagacc gcaaccaatg ccagaaagaa tacctgtcat caaactattg atcagtagac
     1501 tatctacagc aaaatgtata tgaaggctct cccataaatg taaaaagaaa actagcgcaa
     1561 atgttccgac gatcgtccgg agaaaagcgt gttttccaaa aagaaacaat gagataagca
     1621 ataaaggtaa attaaaaagc agtgaagtga cagaaggagc gaatccaaaa agattatgca
     1681 aaagcaaagc aataccagta accccaccat ctgccagctt agatggaata ttgattcctg
     1741 tcacagctaa tccgtagatt cctgcgccta aaatgattcc gcacaattct gtaaataaat
     1801 ttaatttttt catttctagc ctcttttctt ttgaaaattt tcagcaactt ctttattata
     1861 ctgacttata ctcttacttc aagaggaaaa tgaaaaaaaa ttatttttgg ctgtttcttt
     1921 ttatttatca aataaggagt ggaaaaatgc gagcagcaga cttattgaca attcttacaa
     1981 aatcacccaa aaaaacagag tatcaaggag tttttttggc tcaagaacaa aaactggtgc
     2041 ctgtgacaat ggtccagata aataaagcaa atcaatttat ctttttattt gaacacggca
     2101 aagcaccact tgctatgaaa gacatctttc tttttttgat gaagaaccgt gagaaagctg
     2161 tttattatca gtcaaaagaa gaactcattc ctctttatgg agtgaaagag atagaaaata
     2221 aagtggtgat ataagtaaaa ataagaaaac aagacgttta tgatgtcaaa acaaccattt
     2281 tttacatttt tggcaaaaaa gtgtcatgat acagtataat attcgttgaa caggccaata
     2341 aaggtgttct cccccacgag atttctactt tattggctgt ttctttttat taaaataaaa
     2401 gttgtgtgtg taaaaaagat atagctgatt ccaagtgaca atttcgtata gatttttatg
     2461 gtattcgtag tgaaaaaacg agaaccaaaa agttgatccg actttgttgc ttggattctt
     2521 tttttgtcag catatttaga ggtatttttt tctttttttt gttcgtgctg taaggttaag
     2581 gcaagacaag ttagttggga aaaaggaaag aaatgtttat acttaaagaa taacagaaag
     2641 gaaggtcgca tgaataattt tatacaaaca ttaagtgaaa gaaaaggcga gttgctggaa
     2701 gctgcatttc aacatctgtc catttctctc atttctttgc tgattgcagc actgattgct
     2761 attcctttag cgatttgggc agccaatcat aaaaaaatgg cagaagtctt attgcagatc
     2821 acgggtgtgc tccaaacgat tccttcactt gccttattag gtttacttat cccatttgta
     2881 gggatcggaa ctgttccagc attgattgca cttgttattt acgctttgct tccgattttc
     2941 caaaacacgt atattggtct agctgaaatt gacccgtcta ttgaagaagc agcggtagca
     3001 tttggtatgt ccagaatgag acgcttgctg aaagtagagc ttcccatcgc attacctgtc
     3061 attatctcag gtatccgaac tgccttagtt ttaatcatcg gtacggctac tttggcagca
     3121 ttgatcggag caggtggatt agggacattt attttgttag gtattgaccg aaatacacct
     3181 gttttgacat tgattggtgc gatcagttct gccttgcttg caattatatt cagcggactg
     3241 attcgctggt tgcagcataa gaaacctcga tattccatca ttactttagc agcagtggtg
     3301 tgtttatttg gcggcgtttc tttatatcag tctgccttgt ttaaaaatga aaccatcacg
     3361 attgctggaa aattaggagc ggaacctgaa atcctgatcc aaatgtataa agaattgatt
     3421 gaagacaaca caaaaacaga agttgaatta aaaccgaatt ttggcaagac gagttttttg
     3481 ttcaatgcac tggatcaagg tcaaatcgat atctatcctg agttcacaag tactgtccta
     3541 gaaagcttgg tgaaggtacc tgaagcaatg aaaagtgctg atttgacaaa agaaaaaaca
     3601 tatgacgaag cagaagaatt attgaataag caattcgata tgacattatt aaaaccaatg
     3661 gcttaccaaa atacgtatgc cttggcagtc aagagatctt ttgcgaacga acatcaactt
     3721 aaaaagattt ccgatttggc agctatctct gatcagatca aagcgggatt cacactggaa
     3781 tttattgatc gttcagatgg atataaagga atccaagaag catatggctt gaatttttca
     3841 agtgtacaaa gcatggagcc aagtttgcgt taccaagcaa tcaataatgg agatgtcaat
     3901 attgtggatg cctattccac agatagccag ctcaaggagt acgatctagt gactctagaa
     3961 gatgatcgag ggttattccc agcttatcaa ggcgcaccgc tgatgaaaaa agattttgct
     4021 aaagaacatc ctgaaatcgt taaagcactg aataaattaa gcggaaaaat aacagaggat
     4081 caaatgattg atatgaatta ccaagtcaat gtcgaaaaga aaaagccgac agaagtagca
     4141 cgaatcttcc tgaaaaatga aggattagta aaggaggaag atcagtgact aaattaatcg
     4201 agttcaaaca tgtgcaaaaa aagtatgatg gaaagtatgt catcgatgac ctaaatctgg
     4261 caatcaaaaa aggagaaatc tttgttttgg ttggtccttc cggtagtgga aaaacaacta
     4321 ctttgaaaat gatcaatgga ttatctaaac catcagcagg agacatctac ttcaaaggaa
     4381 aatcgttaaa tgaatataat ctgcagaaaa tgcggtggaa tatgggatat gtgctgcagc
     4441 aaatcgcttt gttcccgacc atgacagtca agcaaaatat tgaagtcatc cctgaaatgt
     4501 taggttggga aaagcagaaa agagcagacc gtgtagatga acttcttcaa aaagttggct
     4561 tatctcctga tatttatcga gatcgtatgc ctagagagtt gtctggagga gaacagcaac
     4621 ggatcgggat catccgagct attgctgcaa gcccagatgt gattttgatg gacgagccat
     4681 ttagcgcatt agatccgatt tcaagaaact cattacaaga attggttttg tccttacacg
     4741 aagaattggg cacgaccatc gttttcgtta cgcacaatat ggaagaagca atcaagctag
     4801 gtgatcgtat tgcatttatg aaggatggag aaattatcca atgtgacaca ccagagcagt
     4861 tattgatgaa tcctaaaaac gactacgttc ggcatttctt tgatgaaccg aaacagacga
     4921 aagagtggcg agtagaagat ttagtcgtca atggatattt cttaaatgaa ataccagaaa
     4981 atgctcgtca acaagtttgc tttgatacgc ctatgaaaga agtatattcg cttctttcgg
     5041 tgtatccaag cattgtgata gtagaaaagc aaagaagtat tgggtcgctg acaagtaaag
     5101 aaatctttgc ttttttaagt cgagaggagg aaggaaatga aaacatatga tgcaatcatc
     5161 atcggttcag gtgtgagcgg cttaagtgct gcttatggat tgaaagaagc aggtaaaaca
     5221 gtactagtgg tcgaagaaga tctatggggc gggacttgtc ctaaccgtgg atgtgatcct
     5281 aaaaaagtct tgttaagtgc agtagaggca cgtaatcggg taaaacaatt gagtggaaaa
     5341 ggttttaacg agatcccaac tgccaactgg gaagaacttc aaaaattcaa acgaacattt
     5401 acggatccag tcccagaaag ccgaaaaaaa caattagcag aagctgaaat cgatcatctt
     5461 tcaggaaccg ctcgtttttt ggatgattca tctattgaag tcaacgaaga agtcttccat
     5521 gcggattact tagttttggc aaccgggcaa agacctacga tcttaccagt ggagggaaag
     5581 gaatacttga aaactagtgc agacttttta tcattgcctg tactgcctaa agaaatcatt
     5641 tttatcggtg gtggttatat tgcttttgag ctagctacga ttgccaatgc tgcagggagc
     5701 aaagtaacca ttgtccatca taatcaacgg ccattaaaag aatttgaggc gtctttggta
     5761 gaggaagccg tccatcaaat ggaagcatct ggcattcagt ttgcttttgg tgttgaaacc
     5821 caaaagatca tttcagaagg tactcgctat cgtttagtcg gaaaagagac ggaattagta
     5881 gcagatatga ttttttgtgc gactgggaga cagccgaata cagaatcttt agcacttgag
     5941 caagcgaata tcgtctttga taaacacgga attgcagtca atgattatct gcaaacgagc
     6001 aatccaaaaa tttttgcgtg tggagatatt gtttcaagga aaaccccaaa attgacacca
     6061 gtcgccactt ttgaaggaaa ttatgtagct aagcggataa cggatgcaac aagcgaacct
     6121 atcaaatatc cgattatacc tacgatcgtc tacgccagtc ctaaacttgc tgaagtagga
     6181 gttacaaaaa gtcacgcctc ttcgtcagat caagtggtag agatggatct taccagttgg
     6241 ttcacctatc atcgtgtcaa tgaaccagtt gctaaggctg aattgacctt tgaccagcaa
     6301 aattatttga ttggtgctgc agtgattagc gagcaagcag acgaattgat cgatgatttg
     6361 acactcgtca tcaatcaaaa attgactaaa aaagagttgg atagctatat catggggtat
     6421 ccgacattgg caagtgatct ctcctattta ttgaaataat cagcatgata actaaagcgc
     6481 ttgttgcttt ttccttcttg tgctacactt taaaaaatga atacctgttt ctggggatgt
     6541 cgtagacgac atgagagtaa gttgtgaact tagacccatt gaacctgtca gttaatactg
     6601 gtgaagggag aaacatagca gacgcttgaa gcgggttact atgtcgtgcc catttcaagc
     6661 gttttttgtt attcatatta gaagaaagga ggtaacaaaa tgaagaaaaa tctttatcca
     6721 gcagtcagta tggcattcat tttaattatt tggcagattg ttgtcatagt ttggaaaata
     6781 cctgccttta ttttacccgg acctgctgct gtattttctg cattagcggc agatgcacaa
     6841 gtattacttt cccattctgt cacgacatta aatgaagcag tgcttggttt ggtaatagct
     6901 agcgttcttt cttttattac tgccttggca atggattatt ggaagttttt agaaatgtct
     6961 ttttatccgt tactagtgat ttcacaaatg ttgccaatca tggtgttagg gccattactt
     7021 accctatggt ttggtttcgg tatgcttcca aaagtgatcc ttgttgtctt gataagctat
     7081 tttccgatcg tagtttcttt ttcggataca ttgaggaaag tatcaaaaga gcaaatcatc
     7141 tttttgaaaa cgatgggggc agatacaggg aagatttatc gaatatataa aataccagcg
     7201 ggtttatccg gcttcttttc cggtttaaaa gtggctgcta cttattgtgt cagcggagca
     7261 gtcgttggag aatggctaag cgctcaagca ggcttagggt attacatgat acgcgtaaaa
     7321 aacagctacc agatcgataa agtttttgct gcgatcattt gtgtcattct attgagttta
     7381 ttgctgaatg gcgcttgttc attattgaaa aaagggtatt atcgtatgct ttatattttt
     7441 taagagaagg ggatcaaatg aaaaaattat ggcttttatt accgctactg ttgcttttat
     7501 ccgcttgtgg cactgcaaaa gaaacaagta gcaaacaaga aataaaggat ttgaaaaaag
     7561 tgacacttgt tttggattat gtaccgaata ctaatcatac tggcatctac ctagcaaaag
     7621 aaaaaggcta ttataaagag gctggtttaa atgtccaaat catcgaacca ggagataatt
     7681 cgacaagtat tggtctagta ggtgcggaca aagcacaatt tggtgtaagt tatcaagaag
     7741 atgtcactta tgctcacgca gatggacaga atataccagt caaagcaatt gctacagtga
     7801 tcaaacataa tacttctgga ttcgccaccc tatcagacag taacatccat tcaccaaaag
     7861 attttgaagg aaaaacatat gcaggttggc aaagtccaag tgaagaagct gtcttaaaag
     7921 ctgtgatgga aaaagacggc ggggactttt caaaactgac aatggtagga agcaatggag
     7981 aagggccaga aagcttagga aaatcctcag atatccaatg gtactttgaa ggttgggata
     8041 tgatcaaagc aaaagaagcg ggtattgaac taaactatat cccattgaaa gaattagatg
     8101 aacggttaga ttactatact ccggtaatta ttacgaatga tcaactgatc aaaagtgatc
     8161 cagaattggt acagtctttt atggatgcca cgaaaaaagg ctatcaggaa gctattaagg
     8221 atccaaatga cagtgctaaa ctgttgcaaa aatatgcaaa agaaaatgat cggacgtttt
     8281 tagaagaatc acaagcattc ttgtcaaaaa attatacgga tgatcctgag aattggggat
     8341 tgatggaaga aaaagtttgg cgcaattata cagcttttat gcaggaaaac ggattgatca
     8401 agcaagatgt accttcgcaa gaactattca ctaatcagtt tataaaatag gagtgataga
     8461 tatgtcaatc caaatcagcc acttgaatgt agtcattgaa aacctgaaaa tattagaaga
     8521 catccatttc actatcccta aagatacctt tacatcgatt gtcgcgccaa gcggagccgg
     8581 taagtcaaca ttgctgaaaa ctttaacagg agtccagcct atgacttctg gaacgatcaa
     8641 agtagacgat caaaaagtaa ctggtctcca tacagcattt agttatatgc cgcaagaaga
     8701 tatgttactt ccttggctga gcgtctatca aaacgttacg ttatatcaaa aaatcaatca
     8761 tctaacaatc aatgaagaac aagtaaggga ttatctggat atctttgggt taaacggcta
     8821 tgaacatttt ttgccggaac aattatctgg cgggatgaag cagcgaacgg ccttgttacg
     8881 aacaatcatg aatccttctt cttacttgct tcttgatgaa ccatttggag cgttagatgc
     8941 tatgaccaga gggcagatgc aagactggat gttgaagctg cctaaaaaag caaagcggac
     9001 tactttactt gtgacgcatg atatcgaaga agcaatttat ttatcagaca ggattcttgt
     9061 gctttccgcc cgtccggcac atgtgattgc tgagattcaa gtgcctgaaa aacgacggtc
     9121 acgtgaatgg ctactgcagc aatcagagtt gaagcaaacg atttatcagt tattggcagg
     9181 tgaattagat gttaagtgat gctcattgtc atttagatgg gagtcagagt ttagcactcc
     9241 ttcagcagga acacacgatt ttgacgatca tcaattgtga ttcgccagaa gaatggaagg
     9301 aaaacagaca gcttgcagca agtaagactc aagccttaag ttacggtatc catccttgga
     9361 aagcagatag ctatacgttt gaacaagttg agcctttttt gaaaaaagca aggatcatcg
     9421 gtgaaatagg gttagataat atttggacga atgtaccgat gactacacaa aaaaaagtat
     9481 ttgagcgaca gcttgctttt gcagccataa atgagaaacc tgttgtactg cacacaaaag
     9541 gatgcgaaaa agagatctta aattatatcc aagcttaccc taatcactat ttgatccatt
     9601 ggtattcttc tttggactat caacaagact atattgattt aggctgttat ttcagcattg
     9661 gtcttgattt gaaaaagaat ccagcagtct ggcaactagc taaagcagtc ccaatcgacc
     9721 acttacttat tgaaacggat ggactacatg cagcagagtg ggctttaggg gggatttttt
     9781 cagaaaaaga ttatgtctca ctgcttgaag aacagttaaa gacaatcgct gagatcaaag
     9841 agctatcagt tgaagaagtt aaaaaaaata cttatcaaaa cttggaacgt ttcattagat
     9901 taggtaaata aaaaagagat gtgacacaac tagtgtcgca cctctttttg gctttctttg
     9961 tataaaagta aactgtttga ttaataaatg aatcttagta ccaaccgttt gcttcccaga
    10021 atgctttcgc cgcttcccaa gaaccataac gtgaagtaac atattgttct gctactcttt
    10081 cttggtttgc agcagagtag tcaccattta agtaagaaga atctaattgg taacgtccga
    10141 tgtaacgtcc gttcgttgct gtgtaagaac cgcttgattc tttttgtgcg atccattctt
    10201 ttgcagagct tgtgcttgct ggagtagcag cagtttctgt tacagcagga gcaggttgtg
    10261 tttcagcaac aggcgctgct tgtttttccg tttgaaccgt ttcttgtaca acaggttgtt
    10321 cagcttggac tggtgcttct tgaacggctg cttgaggttc agcagctgct tgtgcagagc
    10381 cttctgttgg gattgttaat tgttgtcctg agta
//
LOCUS       DAXLPI010000091        10104 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXLPI010000091 DAXLPI010000000
VERSION     DAXLPI010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.092"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            268..1560
                     /locus_tag="ACF3T5_002048"
     CDS             268..1560
                     /locus_tag="ACF3T5_002048"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFD3632618.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            2347..3210
                     /locus_tag="ACF3T5_002049"
     CDS             2347..3210
                     /locus_tag="ACF3T5_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFD3632619.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(3339..4961)
                     /locus_tag="ACF3T5_002050"
     CDS             complement(3339..4961)
                     /locus_tag="ACF3T5_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFD3632620.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            5210..5731
                     /locus_tag="ACF3T5_002051"
     CDS             5210..5731
                     /locus_tag="ACF3T5_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFD3632621.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSVLTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(5917..7647)
                     /gene="spxB"
                     /locus_tag="ACF3T5_002052"
     CDS             complement(5917..7647)
                     /gene="spxB"
                     /locus_tag="ACF3T5_002052"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFD3632622.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            7812..8630
                     /locus_tag="ACF3T5_002053"
     CDS             7812..8630
                     /locus_tag="ACF3T5_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFD3632623.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(8733..8930)
                     /locus_tag="ACF3T5_002054"
     CDS             complement(8733..8930)
                     /locus_tag="ACF3T5_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632624.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            9099..9677
                     /locus_tag="ACF3T5_002055"
     CDS             9099..9677
                     /locus_tag="ACF3T5_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFD3632625.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            9772..9909
                     /locus_tag="ACF3T5_002056"
     CDS             9772..9909
                     /locus_tag="ACF3T5_002056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632626.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         3078 a         1814 c         2016 g         3196 t
ORIGIN      
        1 ttcacggtgt tccaaaagta gcttcttcga aaaataagtc gaaatttcac aaaaatttga
       61 gaagcaattt tcgtacattc cttcttattt cttgaagctg accacttctg ttacaacctc
      121 ttttttaata accattctaa aaagataaag ttcgtttttt aagcgttttt ataaaataaa
      181 aataaaaatc attcgatttt atttgaaata tgtatctatt attgatacaa tggttcagga
      241 ttaaataata tagaacgagg tgttcacatg tcatctgtag tagtagtagg tacgcaatgg
      301 ggcgatgaag ggaaaggaaa aatcacagac tttctcagtg aaaatgctga agtgattgca
      361 cggtaccaag gtggcgacaa tgcagggcac acaattaagt ttaacggtgt gacttataaa
      421 ctgcatttga ttccttctgg gattttttat aaagacaaga tcagtgtgat cggaaacggc
      481 gttgtagtca atcctaaatc attggttaaa gaattagctt atttacatga aaaaggtgta
      541 gaaacaagta atttgcgtat ttctgaccgc gcgcacgtaa tcttgcctta tcatatcaaa
      601 ttggaccaat tacaagaaga tgctaagggc gaaaataaga ttggtacaac gattaaagga
      661 atcggacctg cctatatgga taaagcagct cgtgtaggta tccgtattgc agacttatta
      721 gataaagaaa tttttgaaga acgtcttcgt ttgaatctag atgacaaaaa ccgtcaattt
      781 gtaaaaatgt ttgattcaga accacttgat tttgacgata tttttgaaga atattatgaa
      841 tacggacaac aaatcaaaca atacgtcaca gatacttccg ttatcttgaa tgatgctttg
      901 gatgcaggca aacgcgtgtt gtttgaaggc gcacaaggtg taatgctgga tatcgaccag
      961 ggaacttatc cttttgttac ttcttctaac ccagtagccg gtggggtaac aatcggaagc
     1021 ggtgtagggc cttcaaaaat caataaagtt gtcggcgtct gcaaagccta tacgtcacgt
     1081 gtcggtgatg gaccatttcc aactgaatta tttgatgaaa caggagaaca aatccgcgaa
     1141 gtaggacgag aatacggtac gacaactggt cgtccgcgtc gtgtaggctg gtttgattct
     1201 gtagttatgc gccattctaa acgagtttca ggtattacca acctatcttt aaactcgatc
     1261 gatgtattaa gtggattgga aacagtaaaa atttgtacgg cttatgaatt agatggcgaa
     1321 ctaatctacc actatccagc aagcttgaaa gagttgaacc gctgcaaacc aatctatgaa
     1381 gaattgccag gctggtcaga agacattaca ggatgcaaaa cacttgcaga actgccagaa
     1441 aacgcacgta actatgtacg tcgtatctca gagcttgttg gtgtacgtat ctcaacattt
     1501 tctgtcggac ctgatagaac acaaacaaac atcttagaaa gcgtctgggc gcaaatctaa
     1561 ggataaaaac gacgactgaa tcaggacgtt cttaccagag cgagtcgaag tgtttatcta
     1621 tacgataata ataacttgaa gaggtagtga aagagacgtt tagccttatt gccgataggc
     1681 tgtctcttga acactgttta ttcagaataa gggactgtga caaaaaccgt gtcacagtcc
     1741 ctttttccga acaaacggtg ttcaaaagct tgcatctacg gcactaagcc caatcagatg
     1801 aaaaatatgg agtgctattt ttatctgatt gttcttactg cttgatgcag agcagctttt
     1861 tcacaacctc tccactaaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
     1921 tcaacaaaca tgagaagctg ttttttgaaa ttgttccttg tgactcggag ctaaacggca
     1981 ggctcacaac ctcttggtaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
     2041 acggcaaaat atgaggaact attttttccg tttttttctc taatactcag gtcaaaacgc
     2101 tgatttcaca acctctgatt cacggtgttc cagaagtagc ttctacggaa ataagccgaa
     2161 atttcacaaa aatttgagaa gcaattttcg taaattcctt cttatttctt gaagctgacc
     2221 acttctgtca caacctctta ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg
     2281 tctagcgcaa gagatttgct ataatagacg ataagggtaa aatgtaacga gaaggggttg
     2341 aaaaatgtgt atcaaattgt aacagattct tgttgtgatc tgccatacca tgtattagca
     2401 aaagaaaaag ttgatttttt atcaatgaat ctccaattga atgatcaaga gttagtagat
     2461 gacctcggaa agacattcaa ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg
     2521 cctacgacat cacaaatcaa tgttggccga tatttagaat ttttccgttc ttatgcggaa
     2581 aaacagattc ctgttttata tgtggctttt tccagtggat taagcggctc ttacagtagt
     2641 gcgcttcaag cggttgaatt gctgaaggaa gaatatgaag accctgagat ctatgttttt
     2701 gatacaaaag ctgcaagtat tggggaaggg cttttagtat tggaagctgt aagattgaaa
     2761 aaagaaggta aaagcatcga agaaacccta gagtggctgg aaagtaatta tttgaaagtc
     2821 cattcatggg taacagtaga cgatctgaaa tatctagaac gaggcggtag gatttccaaa
     2881 gcaagtgcta ctcttggcag tctattgagt gtaaagccta ttattacggt ggataaagaa
     2941 ggaaggctga cgaatgttga caaagttcga ggaagaaaca agtcgatcca gacaatcgtc
     3001 aatgagacgg taaaaacaat cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct
     3061 ggggatctag agagcgcgga gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa
     3121 gatgtgaagc tttatccctt aggaccaacg atcgccagtc acacaggcta tggctgtatc
     3181 gcgatttttt cgatgggaat cagtcgataa aagcagtaac tcattctgta aaaaagccca
     3241 gaaattgatt gtaacaaaaa cagcgactct taaaagatag atgagcatcc tatctcttag
     3301 aggtcgctgt ttttttattt tttaagctaa ataggccatt atgcccataa ctcgttcaat
     3361 tgtttttgaa tatctgcatt ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc
     3421 acacctttat ccgaaacggc aatgatgcgg tttgccaatg tttggataaa ttgatggtca
     3481 tgtgacgcaa ataagattga gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt
     3541 gattctaagt ctaaatggtt cgttggatca tctaatacaa gtacgttagc tttcgaaagc
     3601 atcatttttg aaagcatgca acggactttc tctccaccag ataaaacatt cactggtttc
     3661 aatacttctt caccagaaaa caacattctt cctaagaagc ttcttaagaa ggtgttatca
     3721 tcttcttctt tacttgcata ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat
     3781 tctttcgttg tatcctttgg taaataagaa ttacttgttg tcacacccca acgtactgtt
     3841 ccagtatctg gcgtaatttc acccatgata actttgaata gcgttgttgt tgttatatca
     3901 ttttctgcga cgaaagcgac cttgtcttct ttgctcaaat taaaagatat atcatctaaa
     3961 attttctttc catcaatcgt tacacttaca ttttctacct gcaataagtc gtttccaatc
     4021 tcacgttcag gtttgaatcc aacgaatgga taacgtctag aagaaggttg gatatcgtct
     4081 aaagtaattt tttcaagcat ttttttacgt gaagttgctt gttttgattt tgatgcattc
     4141 gcactaaagc gggcaataaa ttcttggagt tctttgattt gttcttcttt tttggcattt
     4201 gcgtttgctt gaagtttagc cgctagttga ctagattcca accaaaaatc ataatttcct
     4261 acataaagtt tgattttacc aaaatctaga tccgccatat gtgtacatac tttgttcaaa
     4321 aagtgacggt catgggatac aacgatcaca gtattttcaa aattgatcaa aaattcttcc
     4381 agccaattga tcgattggat atccagaccg tttgttggtt cgtctagcaa aagaacatca
     4441 ggtttaccaa atagtgtttg tgctaataac acttttacct tttgtccacc agttaattca
     4501 ctcatttgct gatggtgcaa ttcttcaggg atgttcagac cttgcagtaa aacagctgct
     4561 tctggttcag cttcccatcc attaagctca gcaaattctc cttctaattc agctgctcga
     4621 ataccatctt cgtcagtgaa atcctctttc atgtaaatcg catctttttc tttcagcact
     4681 tcatacagac gcttgtgtcc catgatgact gtttcaagta cagtatagtc ttcatagtcg
     4741 taatggttct gtttcaatac ggccatccgt tcatctggtc ccatagtaac ggaacctgtg
     4801 gaaggctgca gatcccctga taacacttta aggaacgttg atttacctgc accatttgca
     4861 ccaatcaagc catagcagtt tccgggggta aatttaatat tcacatcatc aaatagtttg
     4921 cggtctgaaa agtgcaaact cacatcattt acagtaatca atttttttcc tcacttttat
     4981 cattattagt tttgacaagt cttatagaat tatatagggg aaaggtaaaa gattcaaggc
     5041 aatcgaacat tctttgtgtt tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga
     5101 gcgaaaatag ctactatagg atttattagt ttgcaacagc ataatttttg acgttattta
     5161 taagagggag tactataaaa ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa
     5221 aagaaccatc gactggggct cattgatttt gggaatttta tttgtacttg tctcattgtt
     5281 atcatttcaa gaccctgtcg gaaatttagt tgcaattgta gtagtctttg cgatttttgc
     5341 ctttattaaa gggatatttg aactgtttgt acggaacaga ttaaaagagc taacaggata
     5401 taaaggaaag atgcccttaa taatcgggat catcgatatt ctagttggcg tctttttctt
     5461 attcaatatt ggtgcaggag tcgcagcatt gccttttgtt tttgcagtat ggtttattgc
     5521 tgattcagtt ttagcgttat ttactgctga cttggcaaga ggagtaagtg aaggatatta
     5581 ttggtttacg atcatcgtca atattttagg aattttatta ggaattttct tattgtttaa
     5641 tccaatttct tctgtgttga cattaagctt tttagttgga ttctatttta tgttatttgg
     5701 tatcacacat atcgtctatg catttagata agtaaaatca aacaaataaa aaagccggct
     5761 tccgattaca gttttactgt tgtaattgga agccggcttt tttatcatgg acaaatatgc
     5821 acgaggtgat ttttaggtgt cgagacaaat ttatgcaaaa atgtgagaag ctatatacac
     5881 aaattttatc ttaatactcg aagcatagcg cagttgtcac aacctctttt catataattc
     5941 attcaatact ggcaaatcat ggacttcata tttttctttg aatgcttgga tttcttcttc
     6001 agaatatttt cttggatcta aatgcagatc ttctactggc aatgggcgct tgttattgat
     6061 cttcacatcg attactacag gacgatctga ttttgcagct gcatcaaagg caaatcgaag
     6121 ttgcgagtat tctgtgactg taaagctatt tgccccgagc gcttctccaa ctttaccgaa
     6181 atctgcatct tctaaaaata caccaaattt cttttgttcc gtatcttctt gttctgcttc
     6241 gatgaaacca aaagaatcat tcgaaaagac aacattaata attggaagct gatatttgac
     6301 ttgtgtaatg atatcttgca tgaccatagc aaacccgcca tcaccactta aggtaaatac
     6361 ttgtttttct gggtagctta gttgtgcggc aattcctcct ggcacaccat tacccatagt
     6421 tgcgaaccat ccagatgtcg tatgtttttg tttaccgttc atattcaata aacgaataga
     6481 atggatagtc gtgttaccga catctgtgac gaaaatcgca tcgtcttcag caatacgatt
     6541 gatttcttta tagacagcct ctggacgaat cggttcttcc tctcgatctt caaaagattt
     6601 caaccaattt acccattttt ctttattttc ttgatttgct aggtaccagc tatcagcagg
     6661 gcgagcatcc cctaactcga caagtctttg caaagctgtg tttgcatcac cgaggatcga
     6721 caaagatgtt tcatggcgtc gtccaaattt tgacgcatcg atatcgattt ggataaattc
     6781 agcagttgga ttaaaaaatg cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag
     6841 atcggcttct gccaatgctt catttccggg tttagtagcg acacgtccag caaaaccaag
     6901 aaagttttca taatcatcag gaataattcc ttttgctaga acggaagcga caacaggaac
     6961 tgaaaaatgt tctgagaatt ctttgatttg cggaaatccg tttcgcgttc cttgaccaac
     7021 ataaagaact ggtttttttg ctttttcaag ataaggcaaa gcagctagca aatctttttc
     7081 ttctggtaat atcacacttg ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat
     7141 ttcttcaaaa ccaagatcaa cagggatagt cacgatagcc acgcctttat gctcataggc
     7201 ggctttaatg gcttcatcta ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt
     7261 gtatacgcta acatctgcga aaataggatt ttcatttagt tcctggaaag agttatagtt
     7321 catggatgta gaagcaactt gtcctaagaa cgctactaga ggcacatggt ccatctgtgc
     7381 atcatacaaa ccattgatca aatgtgtcgc accaggtcct gcagagccga agacagcgcc
     7441 gactttcccc gttaattttg catctgcagc ggctgcgagt gcgccgactt cttcatgacg
     7501 aacttgaatg tagttgattt cggttttttc ttgatatagc gcatccataa ttgaattgaa
     7561 agatcctccg ggaatgccgt aaatatggtc gataccccag ctttcaagaa cttttaccat
     7621 tgcgattcct gcattgattt ttgacatggt gtttcactcc tttagcaatg atgatacata
     7681 aattttttgt atctagagta tagaactttt tatcgaaaat cccaactaat tcgtcttttc
     7741 acaaaaatgt agtcatgttt ttgggaatat gctagagtag tacatagata gattcaagag
     7801 aggggaatgg catggttgaa tggctattga gacttgaacc ttggcagcaa gcacttgctg
     7861 ggacagcttt tacttatttt atgacagctc ttggtgccgg gttggttttt ttctttaaag
     7921 aaatcaaaaa agaagtgcta aacttgatgt taggatttgc ttctggtgtc atgattgctg
     7981 caagtttttg gtctttgtta gatccggcta ttacaaaagc agaagaaaat ggagatattg
     8041 cttggctagt tgtaagtatt gggtttggtt taggcggtgt gtttttatat atggcagata
     8101 aaacactgcc ccatatgcat tttggaccac aacatgaagc agaaggattg cctacccatt
     8161 taaaacggac gatcttgctt gttttttcta ttacattgca taacattcct gaaggtttag
     8221 ccgtgggggt agcattcggt gcagcagcaa ctgcggataa tccgacagcc gctatattag
     8281 ccgctgtttc ggtggcgcta ggtattggta tccaaaactt cccagaagga gcagctgtat
     8341 caatcccgtt gcgacaagaa ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt
     8401 ctggaatcgt tgaaccaatt gctggagtga ttggagcgct actagtaacg aaagtggagc
     8461 tgctgcttcc ttatgcactt gcttttgccg caggagcaat gatttatgtc gtggtggaag
     8521 aattgatccc tgaagcacag cagacattgt ccagcaaacg tcattttgca gtttttggcg
     8581 tcatgagtgg atttatcatt atgatgattt tggatgtggc attaggataa ataaaaaaaa
     8641 tatatatgat atatcaaata gggccgcgaa cttatatcgc gaccctattt ttttagtaag
     8701 ttatatcata atgaagtgaa tggtatcata cattaataat agttttttct atgtgtattt
     8761 cctttgatcc agcgaacaag ccagatgata agtgcgattg ggattagaac tttgaccgca
     8821 aaccataaaa gcccgcttag gacactgaca aaaagactga tgacgaagct agctgcaatt
     8881 agcactaaaa gaaccatgaa gatatttaag aacatatttt tttgacgcat acaaaaccct
     8941 cctcttgttc attctttact atagaaaaag tataacaggg attgtttaaa aacacctcca
     9001 actgaagact gatttttact tcatctactt aaatgatcaa acaattgctt tttctctggt
     9061 ttatcatcta aaatagagcg gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag
     9121 catttacaga tgctgtaatc gcgattatca tgactatttt agttttagaa cttcatgaac
     9181 ctaacggaga cacgtgggag gcgctcgctg cattaggtta ccgatttttt atctatattg
     9241 tcagttttat cattttagcg atttattgga acaatcatca tcatttattt caagttgtac
     9301 ataagataaa tggacgggtc ttatgggcga acagtttctt tgtttttgcg ctttcgcttt
     9361 ttccgtttgc aacttcatgg atcagtgagt acatcaatag cctggctcca gaaatgactt
     9421 atggttttgt taccttgacc gctaatattg cgtattatat cttggcaaaa gaacttgtca
     9481 aagcaaataa aggccacgag aaagcattga aggcgatgtt gaataattac aagaaatcgt
     9541 atctatcgat tggtttgaat gtgttaggaa ttgtcttagg gtatttagta gcgccagtag
     9601 ccgtgctgat cgcaaacatc gtgattctag ctgcttggat catcccaagt cgtaccatcg
     9661 aatcacaata tcggtaaaat gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat
     9721 aaataaacga tcaaatatcc gagtgatact agtattcaaa gagggatgca gatggatcaa
     9781 aaaagaattc atgtaggact gattactagt tttgtcacga tgcttattta tttggatcaa
     9841 ggtcatttga cttggacagt agttggtgtc gtaggatttt tgacattatt cacagcaata
     9901 aaaaaataaa catctttatg taatctgatg ttctatctat ttaagaaaag catgtttaca
     9961 gcagtatgat tctgttgtaa acgtgttttt atttgtggaa aatttaattt ttagaaaagt
    10021 gtatcgaaaa atagatagtt agtttttcca cagtggataa cttcgtaaga gataaaaata
    10081 aaaaattagt caaagaaggg cgta
//
LOCUS       DAXLPI010000092        10022 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXLPI010000092 DAXLPI010000000
VERSION     DAXLPI010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.093"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..722)
                     /locus_tag="ACF3T5_002057"
     CDS             complement(<1..722)
                     /locus_tag="ACF3T5_002057"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3632627.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="ACF3T5_002058"
     CDS             complement(727..1494)
                     /locus_tag="ACF3T5_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFD3632628.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="ACF3T5_002059"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="ACF3T5_002059"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HFD3632629.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="ACF3T5_002060"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="ACF3T5_002060"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HFD3632630.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="ACF3T5_002061"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="ACF3T5_002061"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HFD3632631.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="ACF3T5_002062"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="ACF3T5_002062"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HFD3632632.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="ACF3T5_002063"
     CDS             4894..5211
                     /locus_tag="ACF3T5_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFD3632633.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="ACF3T5_002064"
     CDS             5211..6917
                     /locus_tag="ACF3T5_002064"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HFD3632634.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="ACF3T5_002065"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="ACF3T5_002065"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFD3632635.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8488..8761
                     /locus_tag="ACF3T5_002066"
                     /pseudo
     CDS             8488..8761
                     /locus_tag="ACF3T5_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            8782..9681
                     /locus_tag="ACF3T5_002067"
     CDS             8782..9681
                     /locus_tag="ACF3T5_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFD3632636.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3164 a         1690 c         2006 g         3162 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgatt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctaccct cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagacg aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
     9241 tctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
     9481 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
     9541 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
     9601 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
     9661 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
     9721 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
     9781 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
     9841 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
     9901 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
     9961 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    10021 gt
//
LOCUS       DAXLPI010000093         9902 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXLPI010000093 DAXLPI010000000
VERSION     DAXLPI010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.094"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            72..407
                     /locus_tag="ACF3T5_002068"
                     /pseudo
     CDS             72..407
                     /locus_tag="ACF3T5_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            572..787
                     /locus_tag="ACF3T5_002069"
                     /pseudo
     CDS             572..787
                     /locus_tag="ACF3T5_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397514.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            925..1896
                     /locus_tag="ACF3T5_002070"
     CDS             925..1896
                     /locus_tag="ACF3T5_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3632637.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            2036..3349
                     /locus_tag="ACF3T5_002071"
     CDS             2036..3349
                     /locus_tag="ACF3T5_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HFD3632638.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            3392..4258
                     /locus_tag="ACF3T5_002072"
     CDS             3392..4258
                     /locus_tag="ACF3T5_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632639.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            4255..5103
                     /locus_tag="ACF3T5_002073"
     CDS             4255..5103
                     /locus_tag="ACF3T5_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632640.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            5117..6589
                     /locus_tag="ACF3T5_002074"
     CDS             5117..6589
                     /locus_tag="ACF3T5_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFD3632641.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            6606..7478
                     /gene="scrK"
                     /locus_tag="ACF3T5_002075"
     CDS             6606..7478
                     /gene="scrK"
                     /locus_tag="ACF3T5_002075"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFD3632642.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(7713..9094)
                     /locus_tag="ACF3T5_002076"
                     /pseudo
     CDS             complement(7713..9094)
                     /locus_tag="ACF3T5_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9556..9879
                     /locus_tag="ACF3T5_002077"
     CDS             9556..9879
                     /locus_tag="ACF3T5_002077"
                     /inference="COORDINATES: protein motif:HMM:NF015824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFD3632643.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTHFGSVAKFSK
                     KSILV"
BASE COUNT         3041 a         1695 c         2052 g         3114 t
ORIGIN      
        1 ggaggttttg aatgaatgaa attaagcgca ataaaaattc ttttggtgtt tatttaggag
       61 agcctagctc ggaacagtta aataaaggtg aagctcgtca tgctctatgt cgaaatatat
      121 tttatggtag aaaaggcaaa ctttatcaaa cttattacga cggtatggag gaacagctca
      181 acgcattagg attagtaact aatgctatca tatattggaa ttcactatat ttagaaaaag
      241 tctttgaaca aattaaaacg gaagggttcg actgttccga agaagtgtta cgcaagcaat
      301 ttccattaat tagtgatcac attaattttg ttggcaaata tagtttccgc tatgaaaaag
      361 aattatcgaa tggaatacta cgtccgttaa atatagagaa ttattgagaa tgttcgtctt
      421 ttggttccta ccgtgcattt tcgcacggat gttccagata ctatttttga atgtataaaa
      481 ttgaaaacac aagaatacta ataaatcaac gattcttgtg tttttaattt ttcactaccg
      541 tgcattttcg tttctatgtt ccatgtaccc caatcttgaa aaattaatta aagacttaga
      601 taaagccatt gacgacttag tcgtggtgct accggagtat caatgtttaa ccagtatccc
      661 aggtattggt cctgtttagg ctgctggaat actcgctgaa attggtccaa ttgaacgttt
      721 ttctgatgaa ataaaattga caaagtatgc cggcctatat tggcgacaga gtcaatccgg
      781 taaatctgaa tacgaaaatt tttttacgaa aagcttcacg tctaatatct attttaaaat
      841 aaaaatattc ctgctaattt ggaatcgttt ccttcttttg gtataataat acctgaaatt
      901 cttttgagag gagttgttcg tgaagtgaaa actactatga aggatgttgc tgcgttagca
      961 ggagttggtg tgggtacggt atctagagta ataaatggag taaaggtgaa agaggttacc
     1021 aaagaaaaag taatggctgc aattagtgag cttaattatg agcctgatga atatgccaga
     1081 ggtatgaaaa cgaatagtag caatacaata gctcttattt tacctacgat ttggcatccc
     1141 ttcttttccg agtttgccta ctatgtagag gaagctctca gccaaaaaaa ttataagctt
     1201 tttttgtgta atgcgaacgg cagtgcggaa aaagaaaaag aatatatcca aatggtcaag
     1261 cagagtaaag tagacggaat tataggtatt acctattcag acattgataa atatgtatca
     1321 tcaaatttac catttgtcag tatagaccgc catttttcgg aacaggtttc ttatgtaacg
     1381 gcagataatt atttaggtgg gcaaattgcc gctcaagaac tacttactcg taactgtaaa
     1441 aacttgtgct atgttggggg tgtttcacct taccctaacg aaacaaacaa tcgtaaagct
     1501 ggtttttaca atatttgtga ggaaaataaa atagctgtga aggtattgga tatgcctgaa
     1561 ccaattagca atttagagga acaattgctc gctttttttt cggataatcc tgaaattgat
     1621 agcattttta ccatcaatga tttcatggct ttggatgtga taaaagccca aaaaaaacta
     1681 gggaaggaag cacctaagga ttttcaaatt attggttttg atggagtcaa gctatcgttg
     1741 caaaaagact atttactatc aacgattgta cagccgatag aggaaatggc caaggtttca
     1801 gtagaaacac tcatggcgat gattttaaat gaagaaccgc ctaagcgtgt ggtagtaccg
     1861 gttttctttc acgctggaga gacgactaaa gagtaaaaaa ccggtttata acgatttttt
     1921 tgagataggt tgacagaaag tgatttcaat gatatcttaa aaccaattaa ttggaaacgt
     1981 taccaattaa ttgaaaaata aaacggaaac gtttcaaaaa ataggaggaa catcgatgaa
     2041 aaagagaatg ttagcaattg cagcaatttt agcattaaca gctggaggac ttgcaggatg
     2101 tggcagtaag gagaaaagtt caggtagcag tgatccagga gaaattaaga tttgggttca
     2161 gttttctgat gaaacgcctg aaggaaaagc ttgggcagag attgtgaaca attttaatga
     2221 tgaatacgag ggtgaatata aagctgttac tgagtatatt cctcgtagcg gaagcggcgg
     2281 tggatatgag gataaagtaa atgctgcaat tacaaccaat acccttcctg atgttattac
     2341 gttagatgga ccgaatactg cagcttatgc tcattcagga gtcattgctc cattagatga
     2401 ttatttgaaa gatgcggaca tggatgatgt attagacagc attattcagc aaggaaccta
     2461 cgatggcaaa atgtatgcgt ttggtttctc agagtccagt gtgggtgtct attacaataa
     2521 gtcgctgttc aaagatgctg gaattacaga agatcaattg ccgacgttag aagatccttg
     2581 gacttggacg gaatttcaag caatttgcca aaagctgact gataaataca aaacgcctgc
     2641 aattgatatg caaatggcgt cgggagacga aatgctgacc tacgcatata caccatttat
     2701 atggtcgaat ggcggcgatg tcgttaatgc tgatggaacc aaagccgaag actattttaa
     2761 cggtaaaaaa tccgttgaag cgttggaatt tattcaagaa ttggttgaaa aaggttacac
     2821 cacaatcagt cctattgaaa aaggatttga aactggcaaa tatgccatga tgctgagcgg
     2881 ttcttggaca atggcagatt tagaggaaaa ctatcaagag attgattatg gtattttgcc
     2941 ttaccccgtt tcagatgcaa ctggggagtt agtttcacca accggaagct ggcagttagc
     3001 aatgactgag aaaacagaga aaaaggatgc ttctgcggcc tttattaagt ttgcaaccag
     3061 tacagaatct agtcgtatta tgagtttagg gaacagtgtt ctacctatca gaaattcaac
     3121 agttgattta attaaagatg aagtgtcgga aggaatgcag gtattaatta agcaaaacca
     3181 actttctgga catgcccgcc cagtggttgt agcttatccg caagtatcta gagcattcca
     3241 acaagcagtt caagatatta gctacttcga ggaaaaccca gatgtacaaa aagtagcaga
     3301 tagtcgcgct gctgaaatgc aacaggcaat tgatacttca ctaaaataga agtttctaaa
     3361 gggggccaat caagtgttaa aaaaaccgac aatgaaagac aatttgatag gttacggctt
     3421 tctagcgcca gctttgctgc tgctgacgat ttttctgatc atacccgtaa tcatggtaat
     3481 ttattatgcc tttactgatt attatttgtt aacgccagat gctagacaat ttatcggctt
     3541 agaaaatttt aagcgactgt tcgctgatcc aatttttgta aaaagtattt ggaacacggc
     3601 taaatttgtt gtttggatta ttccggtcca aataggggca gctttaggaa tggcacttat
     3661 tatcaacaaa aatcgtaaag gaaatatctt ttttaaagta gccttttttg cgccagtcgt
     3721 gatgtcctta gtcgttattt cgattttatg gttgtacttg ttaaatccta atgaaggtct
     3781 ggttaatacg ctatttgcaa agattggaat tgcttcgcag ccttttttga ctagtcccaa
     3841 acaagcaatg tatacgcttg ttttcgtatc ggcatggcaa ggtgcaggat atcagatgct
     3901 gctgttttta gggggaatgc aaaatattcc gcaagacgtt tatgaggcag ccgaaattga
     3961 tggtttttct aagtttcagc aatttcgcta tatcaccatg ccattattaa agcccacggc
     4021 gatttttatt ttacttacca cattgatttc ggcctttaaa ctaatcattc agccaatggt
     4081 aatgactcaa ggtggaccaa tgaattctac tatgacaatg gtttattaca tttaccaaac
     4141 tggatttaca gaccgtatgg taggctactc cagctccatt gcgttaatct ttaccactat
     4201 gattggttta attacaattg ctcaaagaaa attggtaaag gaggatgatc cagcatgacc
     4261 aagaaatttg gaccgctaat gatcgtagaa tattgtttgc tgttcctttt agcggcatta
     4321 ttcattttcc cgatgctgtg gatggtagtt tcctctatga agcctgaagc agatgtctat
     4381 caaaatttag cttccttcaa agctttttta cctagtttga atccagctaa ttggtttaaa
     4441 acttatcaag aggttatttc ccggttcagt gtgggaactt atttattaaa cagtattttt
     4501 tatggtttaa cctttgcctg tgggtcaatc attgtaaact ctttggcggg ttttgccttt
     4561 gcgaaggtta atttttcagg caagaaaatt atctttggag ctttgttagc tctgctgatt
     4621 gtcccgatgg agacggtttt aattccgcaa tttaccatcg ttaattcact aggcttagtg
     4681 aatactcgct tggcagtgat tttaccagcg atggccagcg cgtttaatat ctatctgttt
     4741 agaaatttct ttatcgcgat tccagaagaa atcattgaat cagcgaaatt agatggtgca
     4801 aatattgtta aaattttctt tcacatcatg ctgccaatgt ctaaaccagc catagccaca
     4861 gtaggtgtct tatcctttat ctccagctgg aatgattata tttggccgtt aatggtctta
     4921 actgacagca gcaaattttc aatgcaagtg gcaattacta cgatcaatac tacacagcca
     4981 gtttatatca atcaagtgat ggctgtttta accattgcaa cgattccact gattattgtt
     5041 tatatcgttg cacaaaaata tattctgcag ggacttggcg gttctggaac gggtataaaa
     5101 taggagtgaa tagtgaatgt tgcaaaaact tttaaaggcc aatgagttta ttgagaaaaa
     5161 tgctcaaaca gtcaatcaag aataccggaa taaatttcat ttgatggctc cgattggctg
     5221 gatgaatgat ccaaatggtt ttgtttatta tcaagaggag tatcacttgt tttatcaata
     5281 ttatccttac gacagcatct ggggaccaat gcattggggc catgctaaaa gcaaggatct
     5341 aattcactgg gagcatttgc cagtagcact ggcaccaggt gaaagctatg acaaggaagg
     5401 ctgtttttcc ggcagtgcca tagaaaagga tggcaagctg tatttgattt atacaggaca
     5461 caatgttgtg gaagggcaag ttcgacaagt tcagtgtttg gcagtttcag aagatggcat
     5521 tcactttgaa aaatatgccg gaaatccaat catcgcagaa gatcacttag cttctgttgc
     5581 aacgaccgaa gatttccgcg atcccaaagt gtttcagcga aaagaagact attttactgt
     5641 agtcgctact aaaactgcag atgctcgtgg acaaattgtg atgttccatt ccaaagattt
     5701 actaacttgg gagttttatt ccgtattgct ggagggaact ccagaacaag gcattgtatg
     5761 ggaatgccca gacttgtttg aactagatgg caaagaagtc ttgattcttt ctcctattca
     5821 aatgactcca caaggcaatg cttaccaaaa tacgaactca actgttgctt tcatcggtac
     5881 tgtcgattgg caaacaggca aatttctagt agagaatttt catgaaatcg atggcggttt
     5941 agatttttat gcaccccaga cctgtgaaaa cgaagcaggc caaagaataa tgattgcttg
     6001 gatgcaaatg tggcagcgta aaatacctac gaatgatctg ggccatggtt ggtcaggttc
     6061 catgaccttg cctagagagc tgagggtggc tgataatcaa ttgttgcaac ggccagtttc
     6121 atcaatttat caagtaattg atccgcagaa aaggattgaa tgggcgaaga ctgaagagaa
     6181 tcgcttctta ctgaaggact tagtatatga ccagcactat ttacgcttgg aaatcaattt
     6241 aaaacaggca acgaaactag aacttcacta tgcgaaggga cccgaccata cattactgct
     6301 taactatgat gttgaacggg agtgctttac tgcttccagg gaacacatgc cgtatgattt
     6361 atcaggagct gaaaaggaag tgttgaaaaa gcgcagtgtg acgattcctt tggaaaataa
     6421 ttgtctaacc atcgaaattt tccgtgatac ttcagctatt gagctttttc taaatgatcg
     6481 tctgactatg agtacaactt tttatgagac agaaaaagga caggattttc ttttgctggc
     6541 agacagtgaa gtaacctttt cattggaaag cggcttaatt aatctataga agggagagct
     6601 cagtgatgat ttatggttca atcgaagcag gcggcacaaa atttgtgtgt gccattggcg
     6661 atgaagaaat gacgatcaag gaaagagtga gctttccgac aacgacacct gaggaaacaa
     6721 tacctttagt cattgacttc ttcaaacaat accaagcaga tttagcaggg attgggattg
     6781 gctcatttgg gcctatcgat atccatcgcg attccgccac ttatgggtac attacctcga
     6841 ccccgaaagt ggcatggcaa aattttgatt tcatcggtac catgaaaaaa gaatttccga
     6901 tcccgatttc ttggacaaca gatgtcaatg cggctgccta tggtgagtat gtcttcggca
     6961 gtggcaaagg attgagcagt gtcgtttact atacgatcgg tacgggtgtt ggcggcggtg
     7021 ccttacaaga agggcgcttt atcgaagggt ttagtcatcc agaaatgggg catatgctcg
     7081 tcgtccctca tccaaaagat gactttgctg gcagctgtcc atttcatggc aactgtttag
     7141 aagggatggc cgctggaccc gccatcgaaa aacgcttagg gaaaaaagga caagaggttg
     7201 cagaagatga tccttattgg gaaatcgaag cctcctatat tgctcaatgt gcctacaata
     7261 caaccttgat gttttctcca gatgtgatca tttttggcgg tggtgtgatg aaacaacgcc
     7321 atatgctgca aaacgtccac gacgcatttg caaaattggt caatgggtat gtcgaaacac
     7381 ctgagttaag caaatatatc gtgaccccag cattagaaga caacgctgga acgctcggtt
     7441 gtttggcatt ggctcgagaa gcggtcttac attcataaac gaaaaaaggc tcttcgacat
     7501 ttagtgttgg tagagaaaga tacaactgaa aatttagtca taaccagtta ggcagagtgc
     7561 ttaactggtt tttgttttac ttggaagatc aagccttgaa tgagataaat tcttgctcga
     7621 aagttatgga aatttcgata gccgtaggcc acttgtattg tgcaatagtt ttttacaaaa
     7681 aaaagggggt gcggatattt ttttggacct ttttaacaaa tatatctttg cttcttccga
     7741 tattgaagag ggcttagccc tcctaaagat aacttaattc gttgattatt ataccaatga
     7801 atataatcat ccaatttgtt tataaactga ttgattgttg tattcttcca gtcacgttga
     7861 taaaacatct cgttttttaa tcgtccaaag aaaccttcgc acgctgaatt atctggtgaa
     7921 taaccctttt tagacataga gtgaagcaag ttggctttgt tccaatagtc caactaacaa
     7981 tagcaccatc gtaacaatcg acaagtggtg ataagtagat tttccctgcg ggaattctaa
     8041 attcagtaat atctgttaac catttttcat ttggcttctt tgctttaaaa ttccgattta
     8101 ataaattggg gtgtgcagga gaaatttccc cagcatacga agaatacttt tttcgtctag
     8161 aaaatttaac aaatagattt tcctcagtca tcaatctacg tacaactttt tcagaaagct
     8221 ttttacccat attttttaaa gcttggtgaa ttctacggta accatacgca cagaagttat
     8281 gattgaaaat cgtgataatt aattgacgtt ctttgtaata cttatctggt ttctcaagtg
     8341 cttgtttttg ataaaaatag ctgcttttag gcaactggag ctgttgaaga agctctttca
     8401 aggtgaactg atttcgtaag gcatcaatca atatcgtttt ttcttgattt gatagacaaa
     8461 gaagattgac gcctttttct ttttttagca gttcagccgc tccttcgagt aatgtttttt
     8521 cgagctgtaa ttggtgtact tgtttttgta agtcttctac ttcctttaaa agtagctctt
     8581 tttgtttttc ctgatttttt aaagtcatac gcaatggaaa atcctttcca agaaccgtgt
     8641 ttttccattg atatagagta actctactaa ttccttcaga ttctgctaaa tttttcctat
     8701 tagtttgcgg tttatagaat tttttcaaca cagcttcttt ttgatcttgt gttaacttta
     8761 ccgcactttt tcggagtttt ctagctcctg gtgcaagttc ttcacaccgc tgccgcaagg
     8821 actctctatt aggataacca agcatgcgaa tggttcgtgc ataacattga ccatattcaa
     8881 aataatgatt caccgctatt cgtttttgtt cttcagaata ctttgatttt tttcggtgct
     8941 cctgatggag ctctccggat tccaaatact ctttatacca tttccgtagt gcaccaatcg
     9001 acggataccc taattctcga atggttgcag cataagagca atcgtatttt aaaaataact
     9061 gaattgccct tacacgctct ttgtgggaaa acacacggac attcctccta agtttatttt
     9121 ttcgtccagg attatgtccg cacccccttg ctttctgatg acttattctg gttctttgag
     9181 tgtcaatgca tagatgtgat gtagctagtt agaaaaaaat tttgaatact ctttttttct
     9241 ttcagactcc tttttaagta agattttgaa gtgtgagtta cggggtcaga aacttaaaag
     9301 tttttgatcc tttattttca agtctgattt ggcagctttt cggcagctca tttatttatg
     9361 gtcagaaata gatgctgaaa ttttaatcta ttagcaaata gtccagaacg tatgaagagg
     9421 agtcggatat catttgtcat taacaccata aatatttata aaattcgtgt tcacctacaa
     9481 ttaaatttaa tatataatat tcgttgtaag ggcttataat tttttgttaa tgaacaagat
     9541 aggaagaaac atttaatgga cagttttcag agattgtcac tagtaaatca atttgagtta
     9601 ttggctaaat ttgaggacca aaacagatct tattatgaaa gaaaaatcga aatattgaga
     9661 gaaggttacg agtatcatta tgatgatgaa atttggagtg atctttctga gccatttccg
     9721 aaagaagatt ctcgatttgt attagatgta ttgaacatgt atcgagatat caattttagt
     9781 aaatcaaagc taaacttaga tgaaaaccaa attactaatt catactatac tcatttcggt
     9841 tctgttgcaa agttttccaa aaaatctatt ttagtgtaaa attgagaaaa aagacagaga
     9901 gg
//
LOCUS       DAXLPI010000094         9790 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXLPI010000094 DAXLPI010000000
VERSION     DAXLPI010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.095"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            185..1183
                     /locus_tag="ACF3T5_002078"
     CDS             185..1183
                     /locus_tag="ACF3T5_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFD3632644.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="ACF3T5_002079"
     CDS             1295..1804
                     /locus_tag="ACF3T5_002079"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFD3632645.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="ACF3T5_002080"
     CDS             complement(1834..2493)
                     /locus_tag="ACF3T5_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFD3632646.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="ACF3T5_002081"
     CDS             complement(2495..3259)
                     /locus_tag="ACF3T5_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFD3632647.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="ACF3T5_002082"
     CDS             complement(3267..3944)
                     /locus_tag="ACF3T5_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFD3632648.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="ACF3T5_002083"
     CDS             complement(3981..5366)
                     /locus_tag="ACF3T5_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFD3632649.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="ACF3T5_002084"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACF3T5_002084"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACF3T5_002085"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="ACF3T5_002085"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFD3632650.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="ACF3T5_002086"
     CDS             6305..7162
                     /locus_tag="ACF3T5_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632651.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="ACF3T5_002087"
     CDS             complement(7243..7578)
                     /locus_tag="ACF3T5_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632652.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="ACF3T5_002088"
     CDS             complement(7645..9015)
                     /locus_tag="ACF3T5_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFD3632653.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="ACF3T5_002089"
     CDS             complement(9093..9629)
                     /locus_tag="ACF3T5_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3632654.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2891 a         2015 c         1626 g         3258 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac
//
LOCUS       DAXLPI010000095         9724 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXLPI010000095 DAXLPI010000000
VERSION     DAXLPI010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.096"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            37..3591
                     /locus_tag="ACF3T5_002090"
     CDS             37..3591
                     /locus_tag="ACF3T5_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFD3632655.1"
                     /translation="MAVFCQVFFLVGSLTTQAEEITQGTTVSQVSPTSNEVEVQSDLP
                     KETAKEEISTEFVPPTEAPSMNEKSVLDETIGKSQQPGDSPVVAEEIPIETPEVQTIF
                     EKENSSEKSCSTVINEGTESVQSEDSKEATTPIELTKEEMNSNTSLSSEKEEKTALST
                     ESHEPELKKVEESGDTQKEKIKKEAVSSTSSKEVTLIVPQTARSARVRRATNTAVLGG
                     RKIDWGENIGVISSANNPNFTLNYMVRFEINGQVAFCIEPGVAAAEGQVYTATALENY
                     LKDLNIRKKTSLISYLGYVTSKDKSDEQYIAAQFMIWEVLGTKIPIKNIRIDYDRRKA
                     EIQRLLNNFDVRATFHNQTHTVKIGETLKVKSGNDFLQRVKDIQKPKGVNVSIHDNEV
                     WITITKESPEKAKIHFNQIEALGVPLAYRRPGAQTIGVLNPFVPGDSVVNLTILKQGN
                     IRVFKEDAETGVKAQGAASLENAVYGLYQANGKKIKEITLKNINGKVQAEIKDLDPGK
                     YVLKEIKAPKGYVLSDEAIEIVVEPGKTVNTIAKDQVIKGTIDLVKVANKDLVDSTNP
                     DNKPKLAGIEISLTSKTTGKVVKTVVTDNDGYATFGKNTVVFDTYILSETKGKEGYKL
                     FEPFEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKVIALAGTQFKIWDKYANNG
                     KGGYVSMALPNDTEVSDIFTTNEKGYFVTTDTLKYGKNRYELREIKAPEKYVLNKTPY
                     VFSVTDDTNTIQLIYFANRLAKANVEVFKYDTSTNFDQKTAIAGVEFDLFKKDETGTT
                     FVGTFKTDKKGKINVKDLLVGDYFFKEVKPLAGYLALDKDVPFTVTVDKDGTVIALEV
                     ENKRIPPKIGTTAIGKYTNQQKVTPLKKVTITDTVKYSNLIIGKTYTVNGVLMDKEES
                     ITAGKDVPLLVNGKQITASKVFVAKNPNGMISLDFVFDASALAGKEIVVFENVYRDGR
                     LVASHTDIHDKGQTVRIVNPMIHTKATGQNGEKQLQALPNQKVKELVQLNDLIVGQTY
                     KVLVQGYITPDGVKLDGTSAEKIFKADKTTLEFLFEFLVDGRKLAGKGLSFAEWLEAQ
                     SEPKDENDYEVRFEQIANHNVDLKSEDQTVYFVEVPKVPEPKIPEKSQPFKEVKQASV
                     VPMLPKTGSEDTVLWMIIGVLFVLAAGLTFYELRKVKD"
     gene            3691..4629
                     /locus_tag="ACF3T5_002091"
     CDS             3691..4629
                     /locus_tag="ACF3T5_002091"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFD3632656.1"
                     /translation="MKKKERKKRMKKLFFACLFSSVVLAGGINVFADEIGITPVTPPV
                     IEETTPVVPIPDISKIDESVPDQPTPVDPDISSEVTEPTTPVVPDITENSENETPVVS
                     PSTGETTNDKDTTPTTSESYSGTSSVDNDSKIDDFSSSSTTIDTPKETEKKNDLGAPK
                     NLTPENHIKTVSEKEMTVSVSSDGKIVQGTGTEKSIPIVTSNLEEINHVPTSETPLQV
                     ATGQAIVGVQDGVPLVQDNQGNLVEDLSIPVKKLPSGNIEIKTIDGKTKVLPKTGEEL
                     QISLTILGSLLTAVTGGIFYGKKQWRHLRHMFKKTI"
     gene            4648..4998
                     /locus_tag="ACF3T5_002092"
     CDS             4648..4998
                     /locus_tag="ACF3T5_002092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632657.1"
                     /translation="MGLSYVAISQEEVLGKCFNQKHVGSTGERDHRGNMKKVEWEIFQ
                     MNSEKMGEIEVRVAVGGAPKAMRYNAEVLLANPRISRRADVRNIGNDGETNKIARNRF
                     YLLVDRVEVKGGNN"
     gene            4998..5429
                     /locus_tag="ACF3T5_002093"
     CDS             4998..5429
                     /locus_tag="ACF3T5_002093"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HFD3632658.1"
                     /translation="MTLEFDQGIVPGLVKETLGEIYFVKAQRPRMLYEDNENTGEIKE
                     RPVEFSSATQETMFVVNFPPKTNLDGLSFGDVVELEEVEFRFWISIDRESMSNRAEND
                     LKVSALRYKKTGKNVFQTSSSTRATYVKEKQDSNKESAKKE"
     gene            5697..7016
                     /locus_tag="ACF3T5_002094"
     CDS             5697..7016
                     /locus_tag="ACF3T5_002094"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFD3632659.1"
                     /translation="MFQYKGIRIRNRHKYASERFIFYLMLPVLLLMSGFFSLFYTTIT
                     WEWCCIYWIIAFGLAGLFAYMCLYFYFYNVYQRLVHKGKLAKMIHSRRLILERVLKNG
                     RRKVVYYPKIFYHYKNGLIHVRLPLDLGPFQDRFNEMAGQLEEAFFCDLVEEQREKNY
                     ICYTFLYDVEKNRIGIRDITVKDGIITLMKNVQWAFNKAPHAIIIGSTGGGKTYFIMS
                     LIYALLPYGTFDICDPKRADLASLEKIPIFKDQVFYGGGILSCLINLAKETANRYDFL
                     REQPEYELGYDYTYYHLTPHFLVIDEWAAYYASLDTKEKKKAMGCLNQIALMGRQCGV
                     FLILGTQRPDTKYLDGAIRDNFGLRVTLGRVSKAGYKMIFEHTVKSLFNKPIKGRGYI
                     DSGTDQVREFYAPFIDITRFNFFAEFKQLSDQLMAEKIKETKEGKNV"
     gene            7009..7173
                     /locus_tag="ACF3T5_002095"
     CDS             7009..7173
                     /locus_tag="ACF3T5_002095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632660.1"
                     /translation="MYEEIPATCGTRLGIKDLMIDSFLAIVFSEKRLSFFINKKVENR
                     WRVERMYPTS"
     gene            7712..8887
                     /locus_tag="ACF3T5_002096"
     CDS             7712..8887
                     /locus_tag="ACF3T5_002096"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFD3632661.1"
                     /translation="MDGTQLKQMRLSRNLTQKLVADTLGWPQSFLSMIENDQRSCSKE
                     QEEALCEFYANFSSFTSLEIKFDYVRVRVPTNDTKTVVEEILGMDFNSFFYAETRLYG
                     YIEMYQKGMIRVLNSKKGDDKGVLIELSGQGCRNYESVLDERNDSWYQFFTRCVSFNG
                     VSRRIDIAVDDFEELFSLHEFAQKLNRGEFDSKFRTWRFQEEKQLLDNLSSGLSVYLG
                     SSQSLMYFCFYQKNYEIAKKQKLPLEEVEVKNRYEIRLRDAKAVSFIEQYLNKVDFSD
                     LTMGILANQLIMFEGEKNTNVWKPWGKLIQNVGKVNLTVTPEKPNYNRKLMYLKTQPA
                     RTMKIVKTVDEILGLNRFGEILEETELREQDEKIVEDEVRAVYEYIQENPDAIHYLL"
     gene            9392..9589
                     /locus_tag="ACF3T5_002097"
     CDS             9392..9589
                     /locus_tag="ACF3T5_002097"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632662.1"
                     /translation="MGQYFLTGVWYVLKLVIILGIAYICFRILLSGEGMPTPKDFIIS
                     VLNFVADQIQVIIHWAQQRFG"
BASE COUNT         3500 a         1393 c         1991 g         2840 t
ORIGIN      
        1 ataagaaaca aaaagctttg cttgaaagtt gctttattgg cagttttttg tcaagtattt
       61 tttttggtag gctcactgac aacacaagca gaagaaataa ctcaaggaac aactgtttct
      121 caagtttctc ctacttcaaa tgaagttgaa gtacaatccg atttaccaaa agagactgcc
      181 aaagaagaga tatcgacaga atttgttcca ccaacagaag caccttcaat gaacgaaaaa
      241 agcgtactcg atgaaaccat cggtaaaagc caacaaccag gagatagtcc agttgtggca
      301 gaagaaatac caatagaaac acctgaagtt caaacaattt ttgaaaagga aaattcatca
      361 gaaaaaagtt gttctactgt aataaacgaa ggaacagaat cagttcaatc agaagattca
      421 aaagaggcaa cgactccgat tgaattgacg aaagaagaaa tgaattctaa tacttcactt
      481 tcttctgaaa aagaagagaa aacagctctt tcgacagaat cccatgaacc tgaattgaaa
      541 aaagttgagg aatcaggtga tacacaaaaa gagaaaataa aaaaagaagc tgtttcttct
      601 acttcttcaa aagaagttac attgattgta ccacaaaccg ctcgaagtgc acgggtgcga
      661 cgtgcaacca atacagcagt tttgggtggt cggaaaattg attggggcga aaatattggt
      721 gttatttcta gtgccaataa tcccaatttc actttaaatt acatggttcg ttttgagatt
      781 aatggacaag tagcattttg tattgaacct ggtgtcgcag ccgcagaagg tcaagtttat
      841 acagctactg ctttagaaaa ctatttgaaa gatttaaata tacgaaaaaa aacaagcttg
      901 atctcttatt taggatatgt cacaagcaaa gacaaatccg atgaacaata cattgcggca
      961 caatttatga tttgggaagt tttgggcaca aaaattccaa ttaaaaatat ccgtattgac
     1021 tatgatcgtc gaaaagcaga aattcaacgt ttgcttaata attttgatgt aagagctact
     1081 tttcataatc aaacacatac ggtaaaaatt ggcgaaacgt taaaagtaaa aagtggcaat
     1141 gactttctac aacgtgtaaa agacattcaa aagccaaaag gtgttaatgt atctattcat
     1201 gataatgaag tatggattac aatcacaaaa gagtcaccag aaaaggcaaa aattcatttc
     1261 aatcaaattg aagcattagg cgtgccatta gcgtatcgtc gtccaggtgc tcaaacaatt
     1321 ggggtgctga atccttttgt acctggtgac tctgttgtca atttgaccat tttgaaacaa
     1381 ggaaacattc gcgtctttaa agaagatgca gaaaccggtg tgaaagcaca aggtgcagct
     1441 tctttagaaa atgcagtgta tggcttgtat caagcaaatg ggaagaaaat caaagaaatt
     1501 acattgaaaa atatcaatgg caaagtacag gcagaaatca aagatcttga tcctggtaaa
     1561 tatgttttaa aagagatcaa agcaccaaaa ggttatgtac tttctgatga agcaattgaa
     1621 atcgtcgttg aacctggaaa aactgtcaat acgatcgcaa aagatcaagt gatcaaagga
     1681 accattgatt tagtcaaagt cgcaaataaa gatttagtcg attcaaccaa tccagacaac
     1741 aaacccaaat tagctggtat tgaaatctct ttgacgtcaa aaacaactgg aaaagtggtg
     1801 aaaacagttg tgacagataa tgatggttac gctacttttg gtaaaaatac cgttgtcttt
     1861 gatacgtaca ttctttctga aaccaaagga aaagaaggat ataaactatt tgaaccattc
     1921 gaagtcacaa tttctgaaaa tggacaaacc ttccattatg ttttagaaga caaagtaatc
     1981 gaacaacgtc ttaaagtggt gaaagtggat caagaaacag ggaaagtcat tgctctagct
     2041 ggtacccaat tcaaaatttg ggataaatac gcaaacaatg gtaaaggtgg ctatgtatcg
     2101 atggcattac cgaatgatac agaggtttca gacatattta caacaaacga gaaagggtac
     2161 tttgtcacaa cggatacatt gaaatacgga aaaaatcgtt acgagttgcg agaaatcaaa
     2221 gcacctgaaa aatatgttct aaataaaacg ccttatgtct tttcagtaac agacgacacc
     2281 aatacgattc aattaatcta cttcgctaac cgtttagcga aagccaacgt agaggttttc
     2341 aaatatgata ctagtacgaa ttttgatcaa aaaacggcga ttgctggtgt tgaatttgac
     2401 ctattcaaaa aagatgaaac aggcacaacc tttgttggta cattcaaaac agacaaaaaa
     2461 ggtaaaatca atgtgaaaga cttattggtt ggcgattact tctttaaaga agtgaaacca
     2521 ttagcagggt atctggcctt agataaagac gtaccattta ctgtaacggt tgataaagac
     2581 ggtacagtga ttgcactaga agtggaaaac aaacggattc cacctaaaat tggtactaca
     2641 gctatcggta agtatacaaa ccaacaaaaa gtgaccccat tgaaaaaagt aacgattaca
     2701 gacactgtaa aatacagtaa tctaatcatc ggaaaaactt ataccgtcaa tggtgttttg
     2761 atggataagg aagaatctat cacagcagga aaagacgtac cgttgttagt aaatggtaaa
     2821 caaattaccg caagtaaagt gtttgtcgca aaaaatccta acggaatgat ttcccttgac
     2881 tttgtctttg atgctagtgc attagctgga aaagagatag tcgtatttga aaatgtctat
     2941 cgtgacggac gcttagttgc gtctcataca gatattcacg ataaaggaca aacggttcgt
     3001 attgtgaacc ctatgattca tacaaaagca acaggtcaaa acggcgaaaa acaattacaa
     3061 gctttaccaa atcaaaaagt gaaagaattg gtacagttga acgacttaat tgttggtcaa
     3121 acgtacaaag tgctcgtaca aggttatatt acacctgatg gtgtgaaatt agacggtaca
     3181 agtgctgaaa aaattttcaa agcagataag acaactttag agttcttatt tgaatttttg
     3241 gtagatggac gtaagctagc aggcaaagga ttatcttttg cagaatggtt agaagcccaa
     3301 agtgaaccaa aagatgaaaa cgattatgaa gttcgatttg aacaaatcgc aaaccacaac
     3361 gtagacttaa aatcagaaga tcaaacggtt tattttgtag aagttccaaa agtaccggaa
     3421 ccaaaaattc ctgaaaaatc acaacctttt aaagaggtga aacaagcttc agtagtacca
     3481 atgttgccaa aaacaggatc agaagatact gtattatgga tgatcattgg tgtgctattt
     3541 gttttagcag caggtcttac attttacgag ttacgcaaag tcaaagacta agtaggacaa
     3601 acaagcggtt agatgcctaa tcgcttgttt ggttttaaaa aagttattgc ataagtaacc
     3661 tttgaaaaga taagaaatga tctttgtttt atgaagaaaa aagaaaggaa aaagagaatg
     3721 aagaagctat ttttcgcatg tttatttagt tccgtagttc ttgctggtgg aataaacgtt
     3781 tttgcagatg aaataggtat tacaccagtt acgccacctg tgatagaaga aacaacacct
     3841 gttgttccaa ttccagatat ttctaaaata gatgagtctg ttccggatca accaactcct
     3901 gttgatccgg atatatcttc agaagtaact gaaccaacaa cacctgttgt tcctgatata
     3961 acggaaaatt cagaaaatga aacaccagtt gtatcgcctt ctactggaga aacaacgaat
     4021 gataaagata ctacaccaac taccagtgag tcttattcgg gaactagttc ggtagataat
     4081 gattcaaaaa tagacgattt tagttcttca tctactacaa tagatacacc aaaagaaaca
     4141 gaaaagaaaa atgatttagg agctcccaaa aatttaacac ctgaaaatca tataaagact
     4201 gtatcggaaa aggaaatgac tgtttcagtc agttcagatg gaaaaattgt acaaggaacg
     4261 ggtaccgaaa aatcaattcc tattgtgaca agtaatttag aagaaattaa tcatgtccca
     4321 acatctgaaa ctcctttaca agtagcgact ggtcaagcga ttgttggggt acaagatggt
     4381 gtaccacttg tacaagataa tcaagggaat ttggtagaag atttatccat tcctgtaaaa
     4441 aaactaccta gcggcaatat tgaaatcaaa acaatcgatg ggaaaacaaa agtattgcca
     4501 aaaacaggtg aagaacttca aattagttta actatccttg gttctctttt aacagcagta
     4561 accgggggca ttttttatgg taaaaaacaa tggcgtcatt tgcgtcatat gtttaagaaa
     4621 actatttagt aaggagagag aataaaaatg ggtttatctt atgttgcaat tagtcaggaa
     4681 gaagtattgg gaaagtgttt taatcaaaaa cacgttggct cgacaggtga acgagatcat
     4741 cgaggaaata tgaagaaagt agagtgggag atttttcaga tgaactctga aaagatggga
     4801 gaaattgaag tgcgtgtagc tgtcggtggt gcgcctaaag ccatgcgata caatgcagaa
     4861 gtgttactgg caaatcctag aatttctcgt cgagcagatg tacgaaatat tggaaatgac
     4921 ggtgaaacaa acaaaatagc acgtaatagg ttttatttgt tagtcgatcg tgtagaagta
     4981 aaaggaggaa ataactaatg acactagaat ttgatcaagg aattgtccca gggttggtaa
     5041 aagaaacact aggagaaatc tattttgtca aggcacaacg tccacgtatg ctgtatgaag
     5101 ataatgaaaa cacaggagaa attaaagagc gtccagttga attttcaagt gctacccaag
     5161 aaacaatgtt cgtggtgaat tttccaccaa aaacaaattt ggatggatta agttttggcg
     5221 atgtggtgga attagaagag gttgaatttc gattttggat ttctattgat cgcgaatcaa
     5281 tgagtaaccg agcagagaat gatctgaaag tatctgcgct tcgttataaa aaaacgggaa
     5341 aaaacgtttt tcaaacgtct tcctctactc gtgcaacata tgtaaaagaa aaacaagact
     5401 caaataaaga aagtgcaaag aaagagtaat ggtttccatt gctctttctt attttaaagc
     5461 tagaagaagt agctgatgtg gagaaaattg aattggctgg ctatattatt gggataaatt
     5521 tttgaatttt taagaattta gatagtggtt agatacgatt gattctttgt gtattttgta
     5581 ttatttaagt attgtgtggt atttacatta agttaatatt gatgttgtta gtgttaatga
     5641 aggttttctt ttatccaata cattagtcac tctatgatgc gaggagatga gaataagtgt
     5701 ttcaatataa aggtattcgt attcgaaatc gacataaata tgcttcagaa cgatttattt
     5761 tttatcttat gttgccggtt ctgttgctaa tgagtggatt tttttctctc ttttatacaa
     5821 cgatcacatg ggaatggtgc tgtatttatt ggataatcgc atttggttta gctggtctat
     5881 ttgcgtatat gtgtttgtat ttttactttt ataatgttta tcagcgattg gttcataaag
     5941 gaaaattagc aaagatgata catagtcgtc gattgattct tgaaagagta ttgaaaaatg
     6001 gacggcggaa agtggtgtat tatccaaaaa ttttttatca ctataaaaat ggcttaattc
     6061 atgttcgatt gccattagat ttaggtccct ttcaagatcg gtttaatgag atggctgggc
     6121 aactagaaga agcctttttt tgtgatttag tagaagagca acgagagaaa aactatattt
     6181 gttatacctt tttatacgat gtggaaaaaa atcgtattgg gattcgagat ataaccgtaa
     6241 aagatggcat aatcacctta atgaagaatg ttcaatgggc atttaataaa gcaccacatg
     6301 ccataattat aggaagtact ggcggtggga aaacgtattt tataatgtct ttgatctatg
     6361 cattattacc gtatggtacg tttgatattt gtgatccaaa acgtgcagat ttggctagtt
     6421 tagaaaaaat accaattttt aaagatcaag ttttttatgg tggtggtatt ttatcttgtt
     6481 taataaatct agctaaagaa actgctaatc gatacgattt tttaagagaa cagcccgagt
     6541 atgaattagg gtacgattat acttattatc atctaactcc tcattttttg gtgattgatg
     6601 aatgggcagc atattatgca tcgttagata ctaaagaaaa aaagaaggca atgggatgtt
     6661 tgaaccaaat tgctttaatg ggtcgtcaat gtggcgtgtt tttaatttta ggtacccaac
     6721 gtccagatac aaagtatttg gacggcgcga tacgagataa ttttggttta cgtgtaacac
     6781 tgggaagagt atccaaagct gggtataaga tgatctttga acatacagta aaatcattat
     6841 ttaataagcc gattaaaggt cgtggttata ttgattcggg aaccgatcaa gtacgagaat
     6901 tttatgcgcc atttatcgat ataacaagat ttaacttttt tgcggaattt aaacaactat
     6961 ctgatcaatt aatggctgaa aaaatcaagg agacgaaaga gggaaaaaat gtatgaagaa
     7021 atcccagcta catgtggaac tcgattagga ataaaagatt taatgattga ctcttttttg
     7081 gcaattgttt tcagtgagaa gagattaagc ttctttatca ataaaaaagt agagaatcgt
     7141 tggagagtag aacgtatgta tccaacgagt taactgattg gttacagttg ccttatgaca
     7201 attatcgaag gcgaatatga cgggttttta cttcaaataa aagtgctcga tcctatagtg
     7261 accaaattgt cgaaaaagca gcggaacagc ctgtttttta tggacgattc aaaggacttt
     7321 cctgttctta tgttaaaagt aaagaccggt ctcgactgta cctacaagta gaggatatta
     7381 acttgtcctt tgcgttgcca gaaaattaaa tttgtcctca ttagaatacg gaagatgctg
     7441 tgcgtatgct ccacaagcaa acaagcctca gggcggaagg acgcggaagc gtcctagcgt
     7501 tagcgaaagc cctgaggtgc agggtgcgtt gtggggcgtg cgcgtagcgc acgcctaaaa
     7561 cccccatatt ctaacagggg ggtagaattt atattcggtg gcaaagatgg gtgattgaaa
     7621 cattgatata acactatttt gcttcaaaaa ttttgtgata tttttcttcg ctttgtaaca
     7681 taaaatatca caaaagaaag gaaagatata tttggatggg acacaattaa agcaaatgcg
     7741 gttaagtcgg aatttgacac agaaattagt ggcagataca ttaggatggc cacaatcttt
     7801 tttatcaatg atagaaaatg accaacgaag ttgctcgaaa gaacaagaag aagcactttg
     7861 tgaattttat gcgaattttt caagttttac gagtttagaa attaaatttg attatgtccg
     7921 tgtacgagtt ccgacaaatg atacgaaaac agttgtggaa gaaattttgg gaatggattt
     7981 taactctttt ttttatgcag aaacccgctt atatggttat attgaaatgt atcaaaaagg
     8041 catgatccgt gtattaaatt ctaaaaaagg tgacgataaa ggagtgttga tcgaattatc
     8101 tggtcaaggc tgtcggaatt atgaaagtgt actggatgaa cgaaatgata gttggtatca
     8161 attctttaca cgatgtgtct ctttcaatgg tgtgtctcga agaattgata ttgcggtaga
     8221 tgattttgaa gaactatttt ctttacatga atttgcacaa aaactaaatc gtggcgaatt
     8281 tgattcaaaa tttcgaacct ggcgatttca agaagaaaaa cagttgttag ataatctaag
     8341 tagtggctta tctgtttatt taggtagttc acaaagtttg atgtattttt gtttttatca
     8401 gaagaattat gaaattgcaa aaaagcagaa acttccgttg gaagaggtcg aggtaaaaaa
     8461 tcgttatgaa attcgcttac gtgatgcaaa agcggtcagt tttattgaac aatacttaaa
     8521 taaagtagat ttttcagatt taacgatggg gattttagcc aatcagttaa taatgtttga
     8581 gggagagaaa aacacaaatg tgtggaaacc atggggaaaa ttgattcaga acgttggaaa
     8641 agtcaatttg acagtgacac cagaaaagcc aaattataat cggaaattga tgtatttaaa
     8701 gacacaacct gctcgtacaa tgaagattgt aaaaactgtg gatgaaattt taggattgaa
     8761 tcgatttgga gaaattttag aagaaacaga attacgggaa caagatgaaa aaattgtaga
     8821 agatgaggtg cgtgctgttt atgaatatat acaagaaaat ccagacgcga ttcattattt
     8881 gctataaagc ttgcgataag atgggtgaaa catgattaaa acagaagacc aatgatgggg
     8941 agagtatatg attgcttgtt aacgattaat aaaagaaacg atcctatgtg ttggaaatga
     9001 tctacagtta tttaaagaat atttagatta tcaacaatca atcaaaatgt tacatgtaga
     9061 aataggggaa gtattaagct accgtattag tgattcgtta aaatattcgt tgacaaagat
     9121 ttatttatat gcaacacaag tatgtggata tacgaaaaag cagtaaaaac aatgatgaaa
     9181 tatgacgatt tgccaagtaa gatatcctag atttacaaca agtattggaa gaaaaactat
     9241 atctatatat gaaaaataga ggggaaaaga aatgattcga tttttatttt ctgtaggttg
     9301 gcattttttg aaattactgt ttcttttagt ttgtattgtc tgtagtatta tttggttatt
     9361 aacactttaa agaaaggaat atgttggaaa aatgggacag tattttctta ccggtgtttg
     9421 gtatgtattg aaattagtga ttattttagg tattgcctat atttgtttta gaattttatt
     9481 atcgggagaa ggaatgccaa cgccaaaaga ttttatcata agcgtgttga attttgtggc
     9541 ggatcaaata caagtgataa ttcattgggc gcagcaacgt tttggataaa aaaatatatt
     9601 taaaaaattt aatgtttaga agtgggctat gtgatagaaa atttcgagta taagttatct
     9661 agattgccaa agaaaaaggg agctacacaa aaagagctgg ctaataaatt aggtgtcaat
     9721 gtac
//
LOCUS       DAXLPI010000096         9559 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXLPI010000096 DAXLPI010000000
VERSION     DAXLPI010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.097"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            292..2763
                     /locus_tag="ACF3T5_002098"
     CDS             292..2763
                     /locus_tag="ACF3T5_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFD3632663.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2866..4602)
                     /locus_tag="ACF3T5_002099"
     CDS             complement(2866..4602)
                     /locus_tag="ACF3T5_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD3632664.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4599..5303)
                     /locus_tag="ACF3T5_002100"
     CDS             complement(4599..5303)
                     /locus_tag="ACF3T5_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD3632665.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5434..5937
                     /locus_tag="ACF3T5_002101"
     CDS             5434..5937
                     /locus_tag="ACF3T5_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFD3632666.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5897..6235
                     /locus_tag="ACF3T5_002102"
     CDS             5897..6235
                     /locus_tag="ACF3T5_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFD3632667.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6256..7674
                     /gene="ffh"
                     /locus_tag="ACF3T5_002103"
     CDS             6256..7674
                     /gene="ffh"
                     /locus_tag="ACF3T5_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFD3632668.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7786..8364
                     /locus_tag="ACF3T5_002104"
     CDS             7786..8364
                     /locus_tag="ACF3T5_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFD3632669.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8368..8889
                     /locus_tag="ACF3T5_002105"
     CDS             8368..8889
                     /locus_tag="ACF3T5_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFD3632670.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8968..9522)
                     /locus_tag="ACF3T5_002106"
     CDS             complement(8968..9522)
                     /locus_tag="ACF3T5_002106"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFD3632671.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
BASE COUNT         3092 a         1763 c         1987 g         2717 t
ORIGIN      
        1 ttctagtatg attatccatt gagagactcc tttggtagga tgacgttttg gtcgactaaa
       61 ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta gtagaaaatc
      121 gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc tatatgacag
      181 gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt tctatactgg
      241 ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca catgaagaat
      301 aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga taaagtcatc
      361 cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga acgaatagaa
      421 aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca aatcgtattg
      481 gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt gctttgttta
      541 gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac aggaatcgcc
      601 atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt aaatacagca
      661 aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga ccttgtaata
      721 gatgacattg ttattttaga gactggatat caaattccag cagatgcaga ggtcgtggaa
      781 gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat aaccaaagaa
      841 ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta cgcccgatta
      901 gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa gacaatggga
      961 gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg gattgggatc
     1021 atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa tggaaacacg
     1081 atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc tgaaggactg
     1141 tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa gcaaagagtt
     1201 cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct ttgtgtagat
     1261 aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt ctctaaaaaa
     1321 caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta tgcaaaagcg
     1381 atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa aaccactgat
     1441 caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag tgtagcgttg
     1501 gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca attgccggaa
     1561 tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt ctttggtgtg
     1621 tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt aggctatatt
     1681 ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt caaaaaacag
     1741 caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa tgtagctgtc
     1801 caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc agaagaagaa
     1861 tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga acaaaagaaa
     1921 atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga tggcgtgaat
     1981 gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg aaatgaagcc
     2041 gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat gccagaagtt
     2101 gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt gtttttagtg
     2161 aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct gacctatcct
     2221 ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc atctttcttg
     2281 cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa tgtgatggaa
     2341 aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt gatctgcggg
     2401 gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct tttgattgca
     2461 gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag aagtcagatc
     2521 attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa gctgttttca
     2581 atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt ctcggcagat
     2641 tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa aaatgaaaaa
     2701 gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa acgagaaaat
     2761 tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat ctttcgaagc
     2821 aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca ttttcggaat
     2881 ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg taccattaag
     2941 gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg aatgtcggct
     3001 tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct caatgtcgat
     3061 acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat agcggataat
     3121 aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt tgataattgg
     3181 ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc gaatcgtcaa
     3241 ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat aaggttctgt
     3301 ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga tgatttcccg
     3361 aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag aagtcagagt
     3421 ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca gtgaagtcac
     3481 aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct tttctaaacg
     3541 tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt ctgaattagt
     3601 aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga tctcctgatg
     3661 gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg gctcttgaat
     3721 gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta gctgcatctt
     3781 tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc caatcatcag
     3841 ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat acatcttact
     3901 catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat cgggtgtttg
     3961 catgattact tccgcctgat taggattttg caccattttt tttagtacgg gcagcacggt
     4021 tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa gaacaaggaa
     4081 gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg gtaaaaaaac
     4141 gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt tgactggtaa
     4201 tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc tacctaggtt
     4261 cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat tcaatgtttc
     4321 attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc gttcgttcga
     4381 ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt tttcgacatc
     4441 taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct gttccacgat
     4501 ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac taactaaaag
     4561 aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg ttcctgaaac
     4621 ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc tcgctcgatt
     4681 ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc gaagtcaaaa
     4741 ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg tttcatgaaa
     4801 taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc tacggtcact
     4861 tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg ctcagcttca
     4921 tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg ggctaatact
     4981 tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag gccaatgatc
     5041 cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt tttttcttga
     5101 cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc taaaatgatg
     5161 aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt ggcactggtt
     5221 accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga aggttcatca
     5281 tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt ctgtttatat
     5341 tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt ataataggaa
     5401 ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc atgtttaggc
     5461 ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat ggaactagtt
     5521 gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc aattcctaga
     5581 gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca agcaaaagta
     5641 tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat caaagcctat
     5701 caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag cccttcatgc
     5761 ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg tgccggagtg
     5821 gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga atggctgaaa
     5881 actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc tctttgaatt
     5941 ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt atgcagatga
     6001 tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag tatatgacaa
     6061 tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc ttttttctaa
     6121 ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag ataagtaccc
     6181 ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag aataaataaa
     6241 aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca ggcgatggga
     6301 aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat gcgggaaatc
     6361 cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt tactaagcgc
     6421 gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc acaacaaatc
     6481 gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc agagttaaat
     6541 aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc aggtaaaacg
     6601 acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg tccgctccta
     6661 atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt aggtcaacag
     6721 ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat cgtccgtcaa
     6781 gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac ggcaggacgt
     6841 ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt gacgcaacca
     6901 aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa cgtagctgat
     6961 agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga tggggatact
     7021 cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa attcatcggt
     7081 tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc tagccgtatc
     7141 cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta cgacgagaaa
     7201 aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa tgactttatt
     7261 gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa aatgatccca
     7321 ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga tgttgaacgc
     7381 aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga tctgttgaac
     7441 ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga agtgaatcga
     7501 atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa aggggatatg
     7561 aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact aggcaaaatg
     7621 gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa ataagatgaa
     7681 aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta tcagcaattt
     7741 ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag atgtccgtgg
     7801 agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt tcctgaatat
     7861 gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg attgagctgg
     7921 cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt cgatatcaaa
     7981 aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa aggaattatt
     8041 cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt gaaaattcaa
     8101 gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa aatcgttgat
     8161 tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga tgaattagca
     8221 aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta tgctttttta
     8281 gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga agaggtcgta
     8341 cgtatcggca gaaagggcga ataaagcatg cgaaaaatca tattttatgg tgctataagt
     8401 ttagatggct atttggcaac gaaaacggat gatcttcaat ggctttttga cactccaaca
     8461 ggtaagcaaa ctacttatga agctttttat gaaacaatcg atacgaccat catgggcaga
     8521 aaaacttatc aggaagcaaa aaaatatttg gagaccgaag caatttatcc agagaaaaag
     8581 aatttcgttt tttccaccca cgcacaatta caattgccag atgctgaggt cgtaaacgaa
     8641 gatcctgttg cttttctaaa aaatctacaa atgacagaag gaaaagatat ctgggttgtg
     8701 ggtggcggtc gattgatcaa accgctattg gaacatcaaa tgattgatga atggtatatc
     8761 cagattaccc ctgtattatt aggggacggg attcctttat tccaaaaagg aacatatgaa
     8821 gcgcgttttg aaatgctaga cacgacacga ttcggtgaat ttatcgaatt gcattatgta
     8881 cgaaagtaga agtaaataaa ataaaaaagg caactatatg cagaaatgtc ctgtacatag
     8941 ttgccttttt tattaccgtt atgcttttta ctctgttaat ttgttagaaa aatctagata
     9001 cgcatctaaa atcgtttgga agaaacttaa tgttccttca acgatattac cattttcatc
     9061 aattagattc gcaacgtttc cgatatatgc ttccggttgc tgcaaggttg gcacattcaa
     9121 aaagacaagt gattggcgta gatggtggtt tgcgccaaag ccgccgatcc cacctggtga
     9181 tgcagtaacg actaaacctg gtttatgatc ccaaacactg tgtccataag gtcttgagcc
     9241 tacatctaaa gcatttttca atactgctgg cacagaacgg ttgtattcag gagtaacgaa
     9301 aacgacaccg tctagtcccc tcacttcctc acgaaaacga gtccattcag caggagcctg
     9361 ttctggagtt tcgagatcct cattgtaaaa tggcagatca tcgattttta caaatacact
     9421 ttcgtaccct tcaggaagca atttcgcaaa agcttctgct acttttttgt tgtaagaatc
     9481 ttttcttaaa ctaccaataa aaaatccaat tttttttgtc atacgatttt tcctccttca
     9541 aactatctat aataatctt
//
LOCUS       DAXLPI010000097         9487 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXLPI010000097 DAXLPI010000000
VERSION     DAXLPI010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.098"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            17..919
                     /gene="gnd"
                     /locus_tag="ACF3T5_002107"
     CDS             17..919
                     /gene="gnd"
                     /locus_tag="ACF3T5_002107"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFD3632672.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            921..1922
                     /locus_tag="ACF3T5_002108"
     CDS             921..1922
                     /locus_tag="ACF3T5_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFD3632673.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            1919..2203
                     /locus_tag="ACF3T5_002109"
     CDS             1919..2203
                     /locus_tag="ACF3T5_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFD3632674.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            2236..3033
                     /locus_tag="ACF3T5_002110"
     CDS             2236..3033
                     /locus_tag="ACF3T5_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFD3632675.1"
                     /translation="MGYSTNLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            3047..3880
                     /locus_tag="ACF3T5_002111"
     CDS             3047..3880
                     /locus_tag="ACF3T5_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFD3632676.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            3898..4755
                     /locus_tag="ACF3T5_002112"
     CDS             3898..4755
                     /locus_tag="ACF3T5_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFD3632677.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            4777..5286
                     /locus_tag="ACF3T5_002113"
     CDS             4777..5286
                     /locus_tag="ACF3T5_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFD3632678.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            5323..5724
                     /locus_tag="ACF3T5_002114"
     CDS             5323..5724
                     /locus_tag="ACF3T5_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632679.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            5836..6189
                     /locus_tag="ACF3T5_002115"
     CDS             5836..6189
                     /locus_tag="ACF3T5_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632680.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            6223..6666
                     /locus_tag="ACF3T5_002116"
     CDS             6223..6666
                     /locus_tag="ACF3T5_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632681.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            6683..7768
                     /gene="uxuA"
                     /locus_tag="ACF3T5_002117"
     CDS             6683..7768
                     /gene="uxuA"
                     /locus_tag="ACF3T5_002117"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFD3632682.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            7761..8474
                     /locus_tag="ACF3T5_002118"
     CDS             7761..8474
                     /locus_tag="ACF3T5_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3632683.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(8681..8830)
                     /locus_tag="ACF3T5_002119"
     CDS             complement(8681..8830)
                     /locus_tag="ACF3T5_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632684.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            9226..9426
                     /locus_tag="ACF3T5_002120"
     CDS             9226..9426
                     /locus_tag="ACF3T5_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD3632685.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3185 a         1495 c         2107 g         2700 t
ORIGIN      
        1 aaatggaggc gctatcatgg aaattggtgt aattggtttg ggaaaaatgg gattgaattt
       61 agctttaaac ttaaaagagc accatcacaa agtacaagga ctcgacattt caagtacagc
      121 tgttagtcag gcaagagaaa atgacattcc tgcttatcaa gagatctctg aatttctagc
      181 tacttttgag cagaaaaaaa tcatctggtt aatgcttcct gctggagaga taaccgaaaa
      241 catgttaaga aaactttttc caaaactcgg accaggagac attattattg aaggcggaaa
      301 ctcttattat aaagattcaa tccgtcgagc agaagagttt cagaaaaatg aaatcggtta
      361 cttggattgt gggacttctg gaggaatcga aggagcacga catcaagcct gcttaatgat
      421 tggaggagat aaagaaactt ttctatccgt ggaacaatta ttcaaagatg tagcaattga
      481 aagaggatat ctgtatgctg gtgcatccgg aagcggacac ttcttgaaaa tgatccataa
      541 tggaatcgaa tatggaatga tgcaagcaat cggtgaagga ttccaacttg tacaaaaaag
      601 tgagtatgct tttgacttgg aacaagtcgc acgtgtttgg aatcatggtt cggtgattcg
      661 cggctggctg atggaaatca tggaagatca gttaaacgaa catcccaatt taagtaatta
      721 tcggggaatc gtttctgcat caggtgaagc aaaatggacg gttgaaacag cgctagaaat
      781 ggctgttcca gttccagtaa ttgcaactag tttgttcatg aggaatcttt cacaggaaga
      841 agatagtttt tcagcaaaag tagtttcagc actaagaaat gggtttggcg gacatgaaat
      901 agttgaaaag ggtgagtaaa atgccagatt atcatatagc aatcgttaat tccagcagtt
      961 tcggaagagt ctttccacac cacttggaaa gattaaagtc agttggagac gttcatttct
     1021 ttactgttga tcaagagatt ccaggaaaag aactagcgga gttacttaaa ggattcaata
     1081 tcattattgc aagtgttacg ccattttttc gacgagattt ttttgaaaac aaagatgaac
     1141 tgctattgat ttcccgacat ggtattggtt acaacaatat tgatttaaaa gcagcaaaag
     1201 aacaccagac aatcgtatct atcatacctg ctttagttga acgaaatgct gtagcagaaa
     1261 ataatataac taatttgttg tccatgcttc gacgcacgac acaagcacaa gcaagagtca
     1321 aggacgatcg atgggaagat cgggcggagt tcgtcggtag gacgctgttt aacaaaacag
     1381 ttggtgtgat tggtgcagga aataccggaa gcggagtaat cgaaattttg cgtaacggtt
     1441 ttcgctgtga tgttctagct tatgatcctt acaaatcaga tttatacatc gaaagctttg
     1501 gtgcaaaaaa agtagaactt caagaattac tggaacaatc agatatcatt tgtttatgtg
     1561 cgaatttgac agaagagaac tatcacatga tttctgataa agaagtcgat cagatgaaag
     1621 aaaatgttta tctatctaat agtgcaagag gcgctctagt tgatgaagaa gcgatgatta
     1681 atggtctgaa aagcggaaag atcgcaggat atgccacaga tgttttagaa gaagaaccgg
     1741 gacgtcaaga tcatccgtat ttagcgtttg aacaagtagt gattacgcca catatatcgg
     1801 cctacacaag ggaatgttta gaagcaatgg gtgaaaaatg tgtcagagat gtcgaacaga
     1861 tcagtcagca ccttcttcct gaacgttctg tccagacaaa gagcaactat ataaaaggat
     1921 gatgaagaaa atggtgaacg gtttaaaagt gaaaacaggc cctcaatttt atttatatga
     1981 agaaggtggt atttcaaaag tttctgatct cttgaaatca tacggcgcga aaagagtact
     2041 ggtcacccac ggaacggtat catgggaaaa ggcgctacct aaattagtat ttttgaatga
     2101 cgaaacgatt caattttttt atcatcgtta ttctggagaa tgtagttatg ctgaagccag
     2161 acgaatagct acaatcatca aaaaaatgaa atcgatttcg tagttggcgt tggcggagga
     2221 aaactgaccg atctagtggg ttatagtacc aatcttgcaa gaatcgattt cggagtcatc
     2281 cctactttag ctagtaattg tgctccatgg acacctcttg ctgtcatgta taaggagaat
     2341 ggacaagcag aagggaaaac agaacacttt tacagacaag ctgctttttt tataacagat
     2401 ccccaattag tgattgattc ccctgttcca tatttcattg ccgggttagc agatacacta
     2461 gcaaaatggt atgaatctga gctcatctta gaacaagatt ttttgcaaga tgaatcattt
     2521 ttgaagctag cacaagatac tgcgaagatc tgcaaagaag agattttgaa tcattctgct
     2581 aaagcaatcc aagatatggc acaaagaaaa ctgacatcgg agttcaaaca gctttcagag
     2641 attatctttg ctgttgctgg tttagttgga ggattaggag ataaatacgc aagaaatgcg
     2701 gcagcacatg ctatgcatga cgctatcagt aaatatattc cggaaagcca tcgttttcta
     2761 catggagaaa aagtagcgta tgggattttc tatcaactgg cactagaagg tcgctgggaa
     2821 aaaatagacg agttatatcc tttgtatcgg gaattagaac tgccggtttc tctagaacaa
     2881 atgggattat ttccgaaaga taaacagata atagataaaa tggctgaatt tattgattcg
     2941 aaagagaaag tgcatttgat tcctatagag gtaacacaga aatcactgat acagtcaatg
     3001 atggaactgg aaggttatat cagaaagatc tgaaggagga ggacacatgg aaacattaag
     3061 tgtagtacaa agtttgttga ttgctttatg ggtagccgcg gttatgtccc gctggttagg
     3121 tggaggagcc acgttgacct tacgtttttc tcctttgatg actggattag tcgtcgggtt
     3181 gatcatgggg gaagttgcac aagcgatgat tgttacagcc gctttgcaaa tgatttatat
     3241 gggggttttt tctccaggag gatcaatgcc agctgaacca tctattgcag cagctatcgc
     3301 agtaccagtc gcattattag gggatctgtc gccagaagca gcgattgccg ttgcggtgcc
     3361 agtcggattg ctcggaagtt acttgtatca gtttcgtttt ttcatcaata cttttttagg
     3421 taaatataca gacaaagctg tagaagattt gaatacaaaa gctatggccc gatcaattat
     3481 tctttatcca acggttgcct catttttatt attcgttccg cttgttttca ttgctttata
     3541 tttaggggcg ccattgatcg ctgatgtaat caaagcttta gagggaacag tcgttatcca
     3601 cattcttgaa gtagtaggtg gagggttagc ggcaatcggt atcgcgacaa ctgtatacgt
     3661 tatcggtcga aaagattatc tggtcttttt cttccttgct tattttatga gtgttgtttt
     3721 gaaatcttta gatattacga tggttactta tgccattttt gggattatta tcgctttgat
     3781 ctttgtccag tcgcaaaaag ggaaacaagt acagcctgcc acaagcaata acaattcagc
     3841 atcttttgat gacgaagatg actatgatga cggattttaa tttaggagga atgggaaatg
     3901 gctgaaataa tggataatca aacaggtgct cctgaagaaa tcacgaaaaa agatgtaaca
     3961 aaagcctatc tgagatggca ttttgcaaac gaaattcctc actcttttga acgttacttg
     4021 gcgccatcac tactatacgg aatgatgcca atcttgaaaa aactttataa agatgaagac
     4081 tctttaaaag cagcatataa acggcagtta ctgttcttca acactcaatt aagctggggt
     4141 ggcggtgtga ttactggcct aatgtcttct atggaacagc aaagagcaga agaagagtat
     4201 gaagaaaaag agatcttgat gcaggatgac ttgatgtata acacgaaagc gggattgatg
     4261 ggggcactag caggaatcgg agatgcaatc gattcaggaa ccgtccaata tatttttatc
     4321 gcaatcgcgg taccttgggc acaacaggga agtcctttag gcgcactttt tccttttatc
     4381 tgctttgggt tgtatcaggt tttactaggg gtatttttcg caagacaagc tttcactatg
     4441 ggccgtaatg ctacaggcct aatgcaaaac actggtgttc agacagcgat tgaaatgcta
     4501 tcagttttag ggttgtttat gatgggggtg cttgcgggaa actatgtaaa agtagaatct
     4561 tcattgaaat ttgcgatatc cggacgtgag tttgttatcc aggaaattct tgatcaaatc
     4621 gtgccaggga tattaccttt agctgtcgta atgggtgtgt acttgttcta tacgaaaaaa
     4681 ggattgaaag tcacacaagc attattatgg ctgactggaa tattaattgt gctagcagga
     4741 atcggtattt tataaaaaga agggaacgtg aataagatgg gtgtagtcaa tttagcgcgt
     4801 gtggatgagc gtttgatcca tgggcaagta atggtaacgt tatctcaaaa aagtggagtg
     4861 aattccattt ttgttgtaga cgaagttgta gcaaaagata aatttatgag agatctttac
     4921 aaaaatgcag gaagccgtac aggacaaaaa acaattgtca ttacaccaga gaaagctaaa
     4981 ttctattggg atgaatatca atttaaagaa tacaattgta ttttgatcgc aaaaacagtc
     5041 caaacgattt acgacttagt aaaacacgga atccccatgg aagaattgaa tattggaggg
     5101 attgctcaaa aaaatcctga tacagatctt ttagtgacga aatctgttta tttaaataaa
     5161 tcagatgctg aaaaattgaa agaaatgaat gaacaatatg gcgtaaagaa tatttatttt
     5221 caagccactc catctgcacc taaaacagga ttaaaagatg tattgaacaa gtttgatttg
     5281 tcataaacaa atgcttagaa aaaagataaa gagggcgaaa aaatgaagaa gaacgagctg
     5341 tttcgtgatt gggaatttcg ttatcgctat atttaccgta agcgccgtac aaaaaagagt
     5401 aaacagcgtt ttctttcagc attagtatca gatatttaca gtatgagaac agatgtgaca
     5461 gtcattgctt acgatacgct agcctaccga tcaaaaaata tttatgttgg agatatcgaa
     5521 aaagcagaga aagttatctg tacttattac gatacacccg ttcatgcatt aggaagttat
     5581 ttcatgtttg attggaaaga ccaaaggaaa aaaacaatct actctatatt attgtccttc
     5641 attttgttgt tcagtttagg ttggtgggga atgatgatat ataacaaaaa tcctcatcac
     5701 gtattcgatc tgctatccgt ataaacaggt atcactgtac tagcatttgg gagctacttt
     5761 ttcttattgg gaaaagcagc tcgcggatgg cctagcagac aaacttttat tcgaaatact
     5821 tcatccatat taacgatgct agaaatgatc cgtacgattg atgatccgag tgtagcgtat
     5881 gcgtttgttg atgaaggctg ttatggagaa aagggcttag acagcgtgag atcgggtatg
     5941 aaaaaagagg ctattctttt ttatctagac tctgttgggg cagatacacc gctacagttt
     6001 tcgggaaatt atttttcaaa taaagaacag tggctgaaac aagttgataa gttgaaagaa
     6061 aaaaacgtca attatatttt tagtgcaaga aaaaagcaag cgcagttctt ctatttgacc
     6121 aaaacagatt tacgagggaa aacatttaat tggcaaaatg ccaatcagat tattgctctt
     6181 tttcgataag taaaaagtgc aaaacaagga gtgagaagac taatgttgga aatcatgatc
     6241 gttacacatg gtaagttgag cgaaggactg aaagatgcgg cggaagtaat tgtcgggaca
     6301 tcgagccata ttgaaacaat gtgcttagag tctggggagg acatccaaac attcggagag
     6361 aaggtcaaag aaaaattaac tatgatcaac acagcagaac ccatgctagt atttgttgat
     6421 ctaattagcg caagtccgta caatcaagcg ctcttggcag tcaatgaact gactgaagaa
     6481 aagaaagcag ccacttatgt catagggggc gtgaatctgc caatgttgtt agaagcaatc
     6541 aatcatcaga tgcttcacac accgatagaa gaagcggtgg aacaaattgt caaacaagga
     6601 aaagataatt taactgtttg gcatgcttca gcggaagaaa aaaaattaga tgaggacgat
     6661 ttttagaagg aaggctgaaa agatgaagtg gggatttaga tggtatggtg aaaaagggga
     6721 ttcgatccca ttaaaatatg ttcgccaaat accgggaata aatgggataa ttggaacttt
     6781 gctgactaaa atgcctggag atgtttggga gatttcagaa attgaggagt tgaaagcatc
     6841 tgttgagagc caaggactgg aactgcttgg aattgaaagt gtagcgatcc acgatgcaat
     6901 caaggcaggg acagatgaac gagattacta tattgataac tatatacgaa caatcaaaaa
     6961 cttgtctcga tgtggggttc gattgatttg ttatagcttc aaaccaattt ttggatgggc
     7021 gaaaactgat ttgtttaatg aaaacaacga tggaagctat tctcttttat acgatcagaa
     7081 aatcgtcgat cagatggagc caggagaaat gtatcaattg atacacagcc agtcaaaggg
     7141 attcaaattg ccgggttggg aagaagaacg gttgaaaaaa ttcgctcagc tggaaaaact
     7201 gtatgaagga atgaccgaag aagaattatt tgaaaatctg acttattttt tagaaaaagt
     7261 gattccagtg tgcgaaaaag cagatgtaaa aatggctatc catccagatg atccgccatg
     7321 ggaacttttt ggttttccta gagtgacgaa aaatcttggc gacttgaaga aaattctaaa
     7381 tgcagtagat tctccatata atggactaac gctgtgcaca ggatctttag gggcaaatcc
     7441 ggaaaacgat ttagttgaaa tcatccatga ggtaggttcg cgcatccatt ttgtccactt
     7501 cagaaacgtg gagtttttag gagaaagaaa gttcaaagaa acggcacatc cctctacaga
     7561 aggctccctt gatatgtatc atattatgaa agcgttggtg caagaagggt tcgacggtcc
     7621 tatacgccct gatcatggta gaaccgtgtg gggtgaagtg gctatgcctg gatatggcct
     7681 gtatgatcgt gcaatgggac tcacttacat gcaaggtcta tatgaggcga taagtaagga
     7741 aaaaccactg ggagagcatt atgaataaaa tcaagctctt catctttgat atggatggcc
     7801 ttttattaga aactggacgt ttagcttatc gtgcttatgt aaaatcagca caaaagtatg
     7861 attatgaaat gcgtaaggaa gtctattatt tactaactgg ccaaacagaa atggcgataa
     7921 gagaacaaat gggcattttg tacggagaag atgttccata tatcaaatgg cgagaagcaa
     7981 tcaatcaata taaagaaaaa atcgtgaaag aagataagag agtatatacg aaaaaaggag
     8041 cagaagaaat tttatctttt gcaaaagaaa gaggaatcca tacaatcgtg gcttcgtcta
     8101 atacacgggt aaaagtagaa atgtatttaa aaatggaaaa tctatatgcc ttgtttgatc
     8161 aaatcatttc aggggatgat gtaaaaaaag gaaagccgga accagagatc tttttgaaag
     8221 cttgttcaaa aatgaatatt cctccaagtg aagcacttgt atttgaagac tcgattgcag
     8281 ggatcgaagc agcaaggcga gcgggcattt tgtcttttct catcaaagat catttgatag
     8341 ggttgcccga tcataatggg aagcataagt tgaaagtaga tgtaagttta tatgaatcca
     8401 ataaagcaga ctatgaattt tatgacctgc atcaagcaaa acgattttta gaaagtaaag
     8461 ggttgtatct ctaataaaga ggactatttt ctagtcctct ttatttattg tcctgcacga
     8521 ctgagggaac agatgaaaaa gaagtaattt tttattttga agtatctacc ttatttgtag
     8581 tgaacaattt agaacaaaca caaaacgcat ctactgttat ctaccagtag atgcgtttaa
     8641 agagaagaac gagaatcgtc catatgaggg tttccactcc ttatgcagat ttttttaatt
     8701 cttgcactgc ttgttcgatc gtaacacctg tcgctacatg attggtattt tctttgtcaa
     8761 aaacgacaaa tagattcaaa tctgtatcca aggttactcg atattgtata tctttgttat
     8821 agaatgtcat agccattctc ctttctttaa atacttgctg aaaggcaacg aacgcgttga
     8881 taacgaaatt ccatttataa gtataccacc aattgaaagg ttttcatagt taagaaattt
     8941 taaagtcaaa agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg aatcaatcgc
     9001 ggttattcaa ctttttcttc gattcgaaga caatagaaga caatgaattt tatatttcat
     9061 tgcattatcc ttgtgttatt agaaaaagca tgatatatta aatgagtaat ctattttgaa
     9121 acgtaatttg agcgatatat ctacatagat aaaactcctt ttgcctatca aatatttatt
     9181 gcaacgaaaa aacgtacatt gatacgtatc aggaggaatt tatttatgaa taacggtaca
     9241 gtaaaatggt tcaacgcaga caaaggtttt ggctttatca ctggtgaaga tggaaatgat
     9301 gtatttgcac atttttcagc tatccaaggt gacggcttca aaactttaga agaaggccaa
     9361 gcagtgtctt acgatgttga agatggtcaa cgtggccctc aagcaacaaa cattgttaaa
     9421 gcttaataaa aattgacttt taaccgtctt ttcgaaagaa aagacggttt ttttgtacga
     9481 aaaaatt
//
LOCUS       DAXLPI010000098         9208 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXLPI010000098 DAXLPI010000000
VERSION     DAXLPI010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.099"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(11..1816)
                     /gene="glmS"
                     /locus_tag="ACF3T5_002121"
     CDS             complement(11..1816)
                     /gene="glmS"
                     /locus_tag="ACF3T5_002121"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFD3632686.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            2225..2515
                     /locus_tag="ACF3T5_002122"
     CDS             2225..2515
                     /locus_tag="ACF3T5_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632687.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            2528..3553
                     /gene="sppA"
                     /locus_tag="ACF3T5_002123"
     CDS             2528..3553
                     /gene="sppA"
                     /locus_tag="ACF3T5_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFD3632688.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            3556..4215
                     /locus_tag="ACF3T5_002124"
     CDS             3556..4215
                     /locus_tag="ACF3T5_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFD3632689.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            4372..5304
                     /locus_tag="ACF3T5_002125"
     CDS             4372..5304
                     /locus_tag="ACF3T5_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632690.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            5458..6747
                     /gene="tig"
                     /locus_tag="ACF3T5_002126"
     CDS             5458..6747
                     /gene="tig"
                     /locus_tag="ACF3T5_002126"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFD3632691.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            6994..8244
                     /gene="clpX"
                     /locus_tag="ACF3T5_002127"
     CDS             6994..8244
                     /gene="clpX"
                     /locus_tag="ACF3T5_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFD3632692.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            8327..8914
                     /gene="yihA"
                     /locus_tag="ACF3T5_002128"
     CDS             8327..8914
                     /gene="yihA"
                     /locus_tag="ACF3T5_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFD3632693.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(8988..9143)
                     /locus_tag="ACF3T5_002129"
     CDS             complement(8988..9143)
                     /locus_tag="ACF3T5_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFD3632694.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         3032 a         1608 c         1936 g         2632 t
ORIGIN      
        1 tgttctttta ttattctact gtcactgatt tcgctaaatt acgtggtttg tcaacgtcga
       61 atccccgtaa tagcgttgcg taatatgcaa gtaattgagt cggtacgact gtggttagag
      121 tagttagata agggtggaca gttggtagca ccagctggtc actttccttt tctaatccct
      181 ctgagacaat aactagtgtt ttagcgccac ggctttcgac ttctttcagg tttcctcgag
      241 tatgcgcacc agtcacttct tcactgatga ttccgatcac tggtgttcct tcttcgatca
      301 aagcaatcgt tccatgtttc aattcgccag cggcaaaacc ttctgcctgg atataactga
      361 tttctttcaa tttcaaagca gcttcaagag aaacgttgta gtcagcacca cgtccaatgt
      421 aaaaggcatt tcttgtatcg ctgagatata cttctgccaa ctcttctaaa taatcttttt
      481 gggcgatgac cgattccatc gcattagcaa ctaaactcaa ttcatgagtg atatcgaata
      541 caagagaagt ttcaaattct tttttatcac cgatagcttt ggctaataaa gttagtacag
      601 ctatttgtgc agtataagct ttcgttgatg ctactgcgat ttcgggtcca gcatgcaata
      661 gcagtgtaaa ggatgcttcc cgcgaaagag tagaaccagc aacatttgta atcgtaagtg
      721 aagggtagcc gagacggttg atttttacca gcacttggcg gctgtctgct gtttcgccac
      781 tttgactcaa gaaaataaag aatggttttt ttgaaagaag aggtgtgtta tagccaaatt
      841 cactggctaa atgaacctca acaggaatct gtgtcaattt ttccaaagtc tgtttacctg
      901 ctaaccccgc atggtaacta gttccgcaag caacgatata gattcgatcg ctatcagaca
      961 atgtatcgac cagcttttga tcaagagaga cacgattgtc ttctgttacg tatttttgag
     1021 cgatacgacg catgataata ggctgttcgt cgatttcttt cagcatataa aaaggatagg
     1081 tacctttctc gatatctgaa gcatctactt gtgcttcgaa tgaagaacgt tctacgatgt
     1141 cacctgcaaa attttgaata ataacttttt ctgatgtgac ggtaaccatc tcttcatctt
     1201 caatctcaac gaattccttc gtatgagcaa gcatagccat tgcatcgctg gcaatcacat
     1261 tgaatccatc gccgagtccg attaatagtg gacttttatt tttagcgacg taaataacat
     1321 caggtgctgt ccgatcaatc aaagcgaaag cgtaagaacc tttgatgacc cgcaaagctt
     1381 ttaaaaacgc ctcttgcgtt gtagtatcct tcgcgaaagt ttcaattaga tgagcgataa
     1441 tctcagtatc ggtctcacca ataaagtggt catttgctaa ataagcaatt ttaagttctt
     1501 caaagttttc gattacgccg ttgtgaacga gcacgaagcg tccactttgg gaagtatgtg
     1561 gatgggcgtt ttcttcagaa ggttgcccgt gagttgccca tcgtgtatga ccaataccta
     1621 tatttcctgt agtatcaata tttattttgt ttttcaaatt ttgaattctg ccttgtgctt
     1681 tgaccagata atcagttgtg ccatctgaga caaagatacc agctgaatca taaccacggt
     1741 attctaattt ttctagaccg tcaatcaatc ctggtgttac attttttaat ccaatcattc
     1801 caacaattcc gcacataaaa aacatcctac tttctataat gaattcgtat gaatgcttcc
     1861 agctgcagga aaatcctttt gttttatctt tgcagacatt ttttgtggat tttctgtaga
     1921 gtgcagtccc tagggccatc cgccgaatct ttcgataaac cctctcctcg tcatctgttt
     1981 acacagacct ggcgctatcc taaaaggtat aactatcctc ctttttgttt tttatctgga
     2041 aacatttaaa atcataacct tttgcgaatg ttcttgtcaa caaaaaaaga ataaaaaata
     2101 aattggtata tatcaaattc tttgtcaaat gtccttttta tgtcaaaaaa taatgggttt
     2161 ttatctatca tctttttcag gagttcggta caatagaaag taatcggata gaggaggagt
     2221 aaaaatggat tcaaaaattg agatcatgac actgggaatg ttaaaaaaac aattgtccaa
     2281 attcgaagca tcagctggtg tatcagatga tacgaaaata tttttagata ctggctggga
     2341 tagcatccaa gaaatcgcac ctgatgcctt ggaagttgct caagctcgtg agtttacggt
     2401 agaagatgaa ttaacaaaag agtcgttttc cgggtatgcg cgggaagaaa aagcagagcg
     2461 gttcgacact tctgagcagt cagaaactgt gatcgtcata aaaaacttgt attagaaagg
     2521 agaaagaatg aataaaagac gttgggcagc agttctgatt gctgcagggt tatttgtggt
     2581 ttcgttagtt tcagctagtt tgactaagcc tcaggaaaac gatcaaattg agggattgaa
     2641 cagttggtta tatggggatg aagaactagc tccaaatgtg ttggaagagg gagacagtac
     2701 gcaaaaaatc gtaaaattat cagtagatgg aacgattgct gcgggagggt cttcaggttt
     2761 atttgcttct gaaggttaca atcataaaaa ttttatggaa caactgaagg cgattgaaga
     2821 agatccaaca gtcaaaggta tctttttaga agtaaattct cctggtggcg gtgtctacga
     2881 aagtgcggaa atcgctaaga aattggatac gattcgtaaa gaacatgata tcccaatgta
     2941 tgtcagcatg aaaaacatgg cagctagcgg tggctactat atttctgctc aagcagataa
     3001 aatctttgca acagaagaaa cagtgaccgg ctcaattggt gtgatcatgt caggattgaa
     3061 ttattcagga cttctagaaa aactgggtgt ggaagataca acagttaaaa gcggagcgtt
     3121 gaaagatatg ggttcaggaa cgcgaccaga gacaaaagaa gaagaagcgg tgcttcaagc
     3181 atatatcgac agcgcatata gtcgttttgt agcaatcgtc agcgagggac ggaataaatc
     3241 tgaagaagaa gtcaaaaaaa ttgctgatgg aaggatttat gatggtgtcc aagcaaaaga
     3301 agtaggttta gtcgatgaat tgggatttcc tgaagatgct ttaaaagcta tacgaaagga
     3361 acaacagctt gaagatgcgg aactagtaga atattcttcc acgccatctg ggtttgctag
     3421 tacatggtta ggaagtaaac tagcagaatt ccagggattg aagccttctg agactagtca
     3481 gatcttatcc gttcttgaga atctagggac agcggaatca ccaaaagcaa tgtattatta
     3541 cggaggtgag taagcatgtc agagctttca aatgaaaata aggaacaaga aaaagataag
     3601 caacagcagc ctgctgtagg acctgactcc aacttttccc aaaaggtact gagctctttt
     3661 gaaagagagc ctttgacgcc cgaagaaatc aaaaagaaac aacgtaaatg gaaaaagtat
     3721 acggacacaa gaaatgaacc gccaaaagca aatgattttc ctaagtattt ctatgcaggt
     3781 ttttttatcc gaatgtttgc cttcttggtc gatcttttgt gtatcggagc tattacgcgt
     3841 ttaacacttg gtattgccaa aaatctaggt tggcttaatt tttcagattc ttatctgagt
     3901 ctctacggat tcacagcctt acttatttat ctagcgtatt ttattttatt gacgaaattc
     3961 aatcatggcc aaacgattgg gaaaatgatc tttggtattc gagtcatcag ctttgatgaa
     4021 gaagaactta gctggtcaac cgttttagtt agagaaggtg cttgccggtt cattctaaaa
     4081 tttcctttgc tgatgttagg gtatcttcca gcagtcttta atcagaaaaa gcaacatctg
     4141 ggcgattttt tcactaatac aagtgttgtt acgatcaatc tgatcaaagc gttcaaccag
     4201 gaagtcaaca gctgatgagc aataaaaagc agtaacagtt gaaagcacct tctaaaagtc
     4261 agatctaaaa tctagctttt agagggtgct tcagttattt ttaattgtca tgcttttttt
     4321 gttttgctac tattgaacat gagctgaata tgagcaagag gaggacactg cgtgaagccg
     4381 attgattttc cagaaaaata tgagaattta atgcgagtgg cacaacaagc actagcaaac
     4441 cagcaatatc agcaggcaaa agaattattt caacgagctt acgagttgaa agaaagcttt
     4501 gaggctaata gcctgcttgt tttttgtctt tacgagttgg acgagaaaaa agaagcattg
     4561 aagcaagcac tcttgcatga aaaacagtat cttcaaaatg aggagtttgc tgaattttat
     4621 tttgacttgt taatcagtac acaagatttt ttatatgcta gaaaattgat agcaagtaca
     4681 gatttttatg aagctttcga gcagcgggtc attgaaaaaa tccaatttgc agaagaatta
     4741 tccggacaaa tggagaggca gaagatcaag gcactccaca ataaatcgga ggaacttcct
     4801 tcgttggaac ctgcacgtca gcttgcgtct attgatgaga tcgaacagct tccttatcat
     4861 gagtttatcc aaacagcgag taaacttatt gtatcgccag aggtccatat tttagcaagg
     4921 gcaaaattac tggagacact taggcaattg aacgaatgcc atcctgtttt ctatttgacg
     4981 attgaagaaa agttagtcaa agtcatacca aaagatctgc caaagccgca gcagcaggca
     5041 agttaccagc aattatgtgt attttcagat cgttacggga atgaagatgc gctgcttagt
     5101 tccgtgttaa aagaagaatt cacgttgcaa tctgcaattg tttatcctgt ctatgataca
     5161 tacatcgaag acccgaagcg ctggtttcaa ctgactgtag aggcgtgtac aggaaaaacg
     5221 atgtatgaag gtacagaaga tgaaaaacaa gattttttaa aaaaaagaga aaaaattttg
     5281 cagcaaatga tcttcttcca ttagatttta tcagtgaaaa agataaaaat gttatgaaaa
     5341 acaaggattc tatccattca atatgtttac aatttgaaag ggttagtgta ctatttaaag
     5401 gtatgcatga aacgtttttg atgtataaaa tgaatttcta aactcggagg gaattaaatg
     5461 actgcaacat ttgaaaaaaa aggcacgaat gatggtgttt taaaatttac gattgcgcaa
     5521 gacgaaatcc aaaaaggatt aacaactgcg tttaataaag tgaaaaaaag cttgaacgta
     5581 cctggattcc gtaaagggaa agtttctcgt caagtattca accgtatgta cggtgaagaa
     5641 gctttatatg aagatgcatt gaatgctgta ttaccagaag cttatgaagc tgctgtcaaa
     5701 gaagctggca tcgatcctgt ttctcaacca aaaatcgatg ttgaaagcat ggaaaaaggt
     5761 caagactggg taatcactgc tgaagtaaca gtgaaaccag aagtaaaatt aggacaatat
     5821 aaagatctag ttgttgaaaa acaagaccgt gaagtaacag atgctgacgt ggatgcacgt
     5881 atcaaacgtg aacaagaagc acaagctgaa ttagttatca aagaagatgc tgcagctgaa
     5941 aatggcgata cagtagtaat cgacttcgaa ggctttgtag atggcgaagc gtttgatggc
     6001 ggaaaaggcg aaaattactc acttgaatta ggttcaggct cattcattcc aggttttgaa
     6061 gatcaattag ttggtcataa atcaggtgaa agtctagatg taaatgtaac tttcccagaa
     6121 gattatcaag cagaagatct tgctggaaaa gaagcagtct tcaaagtgac aatccatgaa
     6181 gtaaaagcaa aagaagtgcc tgaattagat gacgaattcg caaaagatgt tgatgacgaa
     6241 gtatcaacat tagacgaatt gaaagaaaaa tatcgcaaag aattagtaga aacaaaagaa
     6301 aaagcagcgg acgatgcaaa agatgaagca gcgatccgta tggctgttga aaacgcagaa
     6361 atcgttgaat tgccacacgt gatggttcat gatgaagtac atcgttcaat ggacgaattc
     6421 ttgaacaaca tgcaacgtca aggtatctct cctgaaatgt actatcaatt aactggttct
     6481 acagaagaag acttgcataa acaattcgaa ggtgaagcag aaatgcgcac aagaacaaat
     6541 cttgtgatcg aagcaattgc agctgctgaa aatcttcaag caacagaaga agaaatcaat
     6601 gcagaaatcc aagaattatc agaaacatac aacatgccaa tcgaacaaat caaacgcgtg
     6661 ttgacagaag atatgctgga acatgatgtg acaatgaaaa aagcaatcga attgatcaca
     6721 ggtacagcga cagaagcgaa agaatagaaa tcaagtaata aatcatttct gaagaagcta
     6781 cttctagaaa attgtttatt cgaaataagg gactgtgaca cgattttgtc acagttcctt
     6841 attttttgtt aagagtaaaa aatgcaaagg tataggtcag ttgatccttt gttaaacact
     6901 ttttcttttt actaaactat gttatgatag taaaagactg taaagtcttt gatagtttct
     6961 tgcatacttt ttgcaagtta aggggtgaag accatgtatg acaatccaag tagcaatgag
     7021 accgtacgct gttctttttg cgggaaaacc caagaagaag tgaaaaagat cgtagctggt
     7081 ccaggggtct acatctgtaa tgaatgtatc gacctatgta aagaaatcat tgatgaagaa
     7141 ttttatgaag aagctgtacg cgaattcact gaagtcccta aaccactaga aattcttgaa
     7201 gtattgaata actacgtgat tggacaagat cgcgcaaaac gctcgttagc tgttgccgta
     7261 tacaatcact ataaacgtgt caatcagcaa gcacaagaaa acacagatga tgttgaactg
     7321 caaaaaagca atatctgctt gatcggacct acagggtctg gtaaaacatt tttagcacaa
     7381 acattagcaa aaacattgaa tgtacctttt gcaatcgcag atgcgacaag tttgacagaa
     7441 gctggttatg tgggggaaga tgtagaaaac attttgctta aattgcttca atctgctgat
     7501 tacaatgtgg aacttgcaga aaaagggatc atctatattg atgagatcga taaaattgcg
     7561 cgtaaaagcg aaaatgtatc tattactcgt gacgtttccg gcgaaggtgt ccaacaagcc
     7621 ttgctgaaaa ttctagaagg aactgttgca agtgtgccac ctcagggcgg acgtaaacat
     7681 ccacatcaag aatttattca gattgatacg acgaacatat tgttcatcgt aggtggtgca
     7741 tttgatggca tcgaaacgat tgtgaagaat cgtatgggcg aaaagacgat tggatttggt
     7801 acacaaaacc aaaaactttc tgaagacgaa agtgtcatgc agcatatcat tcctgaagat
     7861 ttactaaaat tcggattgat ccctgagttt atcggacgat tacctgttat ggcagcttta
     7921 gagaaattga caacagatga tctggtacgg atcttgactg agccgaaaaa tgcgctagtc
     7981 aaacaatatc aaaaacttct ttctctagat aataccgaac ttgaattcga acctgaagcg
     8041 ttgagagcca ttgcgaaaaa agcaatcgaa cgcaataccg gtgctcgagg acttcgttcc
     8101 atcattgaag aaatcatgat ggatgtgatg tttgatatcc cttcaaatga aaacatcgaa
     8161 aaagtgatta tcacaaaaga agcagctgaa ttgtctggtg agccaacagt aatctataaa
     8221 gattcagaga aaaaagcggg ttgatgaaaa gaggctgccg taaaagtcat tttggctttt
     8281 gcgacagccg cttgctttat tcatcacctt gttaaggaga gagaccatga aagtccatca
     8341 agctgaaatt atcataagtg cagtggcacc aaaacaatat ccagatacac aattgccaga
     8401 aatagctctg gcaggacgat caaatgtagg gaaatcttct tttatcaaca cattgattga
     8461 tcgaaagaat ttagcccgta cttccggtaa gccaggaaag acccagacat tgaattttta
     8521 tttgatcgag aacgcattac attttgtcga tgttccggga tatgggtatg caaaagtatc
     8581 caaaactgag cgagcaaaat ggggaaagat gatcgagaca tatttaacga ctagagacca
     8641 gctacgtgcg gtcgtttctt tagtagattt gcgtcataag ccttctcaag aagatatcca
     8701 gatgtatcaa tttttgaagt attatgatat ccctgtaatt gttgttgcca ctaaggcaga
     8761 taagatccct cgtggtaaat ggaacaagca tgagagcgaa atcaaaaaag cgttggattt
     8821 tgatcgcaat gacacgttta ttctcttttc ttccgttaca aaacagggaa aagaagaagc
     8881 gtgggcagca atcgaacaag caattgaatc ataaaaagaa taaaaaagac ttaccagtca
     8941 aactctttca taaaagtttg gctggtaagt ctttttttga gcttaactta cttttgtttc
     9001 ttgtcttttt tcttttcatt ttgtggctgt ggttttagaa atcgatcgat ctcttctttt
     9061 ggtgcaactg ttttttccgg atcgacagca aatgattcga aaagtttatc taaattatct
     9121 gtacgttcaa atttcaaccc cattgtaaac acctctttgt ttctttttac ttagtctatc
     9181 atgtaatcgt ccaaattggt ttctaaga
//
LOCUS       DAXLPI010000099         9194 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXLPI010000099 DAXLPI010000000
VERSION     DAXLPI010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.100"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            236..1042
                     /locus_tag="ACF3T5_002130"
     CDS             236..1042
                     /locus_tag="ACF3T5_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632695.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1054..2274
                     /locus_tag="ACF3T5_002131"
     CDS             1054..2274
                     /locus_tag="ACF3T5_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632696.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2264..3850
                     /locus_tag="ACF3T5_002132"
     CDS             2264..3850
                     /locus_tag="ACF3T5_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3632697.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3889..6027
                     /locus_tag="ACF3T5_002133"
     CDS             3889..6027
                     /locus_tag="ACF3T5_002133"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD3632698.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            6315..7706
                     /locus_tag="ACF3T5_002134"
     CDS             6315..7706
                     /locus_tag="ACF3T5_002134"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFD3632699.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            7765..8574
                     /locus_tag="ACF3T5_002135"
     CDS             7765..8574
                     /locus_tag="ACF3T5_002135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3632700.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            8546..>9194
                     /locus_tag="ACF3T5_002136"
     CDS             8546..>9194
                     /locus_tag="ACF3T5_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD3632701.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNND"
BASE COUNT         3155 a         1416 c         1864 g         2759 t
ORIGIN      
        1 aaacgaagaa aaagtgttag tagaaaatcg ccttcgattt tccaccaaca caaaatatta
       61 tagaacccct tttttggttt attgcaattg tctagcatct tctgtaaaat agaaacgtta
      121 gagaaaatct tgtatcatag aaaattaaga aagaaataag aacgcagctc ggtaccaaaa
      181 atatggtaaa atggcacttg actgtaattc actgaaatcg cttggaggaa cacaaatgtt
      241 taaagatttg tatttattta tgaaagacat ttatcagaat agaaaattac tgctacagtt
      301 ttcgatcaat gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt
      361 tatcaatccg ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc
      421 acaaatgatc gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg
      481 gttcttcttc tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt
      541 agtaaagaaa gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct
      601 gtacacccat ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata
      661 tccttcagca atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac
      721 aggattgact tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat
      781 tacgatcgtg ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt
      841 tgattcaggt cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta
      901 tcgtgagtca tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt
      961 ctgggttttc acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc
     1021 acatttttca gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa
     1081 gatatcacaa aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc
     1141 aatcctttta agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa
     1201 ataaaaaaag gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg
     1261 ttgaaaatca ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa
     1321 atctcagctt tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat
     1381 atctatttaa atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat
     1441 gatatcattg aatttgctga tattggcgac catatttatc aacctgtaaa aacctattca
     1501 agtgggatgt tcgttcgtct agcttttgct gtagcgatca atgtgaatcc tgatattttg
     1561 attgtggatg aagcattggc agtaggagat cttgaattcc aactgaaatg tatggaaaaa
     1621 tttacagaaa ttaagaactc gggaaaaacg attttattcg tttctcatga tattagttcg
     1681 attcgccgtt tttgtgatcg ttcctattgg ttgaaaaatg gagaagttgt cgaatatggt
     1741 gatacgatgg aagtaacgac gaattacgaa aactttttga agaaaaaatc agttaaaaca
     1801 gtagatcgaa ataaaaacca agtagaacgc ttcgcagctc cagatatcgt agacgtgacg
     1861 aaagccgaat tgttggataa aaataaaaat ccagtggata tgctggaaca aggagaaaaa
     1921 ctatacgtta aagtaaccta tgaagtaaaa gacgatacaa tcaaaaatcc agttttagga
     1981 attgctctgc gtacagtcga caatttttat gtgtgcggac tcaatactct actagatggt
     2041 atcaagatcc cttggaaaaa aggtgagaat atcttttatc tggagtatga aaagttaggt
     2101 ttgttagctg gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct
     2161 ttggtataca agacaaaata catgaacttg tttgtcagcg gttcttatat cggagaaggc
     2221 atcgtggtct tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga
     2281 aatggaccta gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag
     2341 taaagtttta gagtttggtc ccggaaacgg tcgattaacg aaacatttga tcggagcaaa
     2401 acaatgtgaa gtgagcattg ttgagctaga taaggaatta ttcgattttg tcagcgaatt
     2461 tgcccaagat ggattttatg gagatatcga aagttttgaa tgggcaaact attatgctgg
     2521 acagacattt gattacgtct tgtttgcgga tgttttagaa catttagtag atcctggaaa
     2581 aacattgaag aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc
     2641 taacttagca cataattctg tcatgatcga tttgttcaat aatcaattgc cttgggcttc
     2701 ttatggtctc ctagatgaaa cccataattc tttttatact catgatggtt tccaaaaagt
     2761 ttttgaaaaa gccggattat tcattaatat tgaagattac ttgtacttgg ctgtaggaga
     2821 tacagaatta aaatcaactt atgaagagtt gccggaagct gtacgctatg atttcaaaat
     2881 gcgaccattt ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagtaca
     2941 aacttccatt gcacagccac aaaactctaa ttatgtaaaa gtactagaag taacccagca
     3001 aacgaaacaa gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga
     3061 gacactaagt ttccccgtgt cagaagcaac agaaagaatg gtgtttcgct tcgcacagca
     3121 acctagcttt atcgaattca gtgcagaagc tgctggagaa aaactcatat ttatcgattc
     3181 aaatgctgta gtcaaaacag taaatgattg ttatttgttt gatgggaaag aaataccaga
     3241 gttcgctcta acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat
     3301 cggggaattg acaccaacaa tgaaagaatt gctggaaaca attcgtccta tggctgaagt
     3361 cacaaagcag ctttctgaga aaaatgatga attagaaaga gaaaatcagc atcttcttga
     3421 ggaaaataca agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt
     3481 ggaaagtgat gaatggacga tcaaacatag actaggacga cgtaagaaaa aaacatcgaa
     3541 aaaaatacaa gaaaaagagc tttctatatg tattgatgag aaaatatggg atgcagaaac
     3601 aaaaatatta aaaatcatcg gatggggaat cgctaattcg gatcgccagc cattgtccta
     3661 taagttatca gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagatgaagt
     3721 caatcaagca gaacagctcg caaaaggaac agaagcggga tttgaattac gaatcctttg
     3781 cgagcaagaa cgttcattct tagtggaagc agtagctcaa aatggtcaat catggatcat
     3841 tgaaatgtaa ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa
     3901 gaaattgccg tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt
     3961 gtgggctggg caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa
     4021 aatgtgattg ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt
     4081 gatgttaaga cccaaagcgg ttttaagata cgtttatccg ggaaaatgag aggaaaagcg
     4141 atcttagatt ttcaaacggc aaaacatcaa aatggcatag ctgtgaaact aaatggtaaa
     4201 tacccttatg atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa
     4261 gggataaatt atgcaaggac tcatggcgta aaaaaaacaa tccgccgggt caaactggaa
     4321 ttgaaaccag gatcgatcga ctatgcagag tggatcagtc gtcacgagaa acctgatctg
     4381 aaagagcaaa ggaaagaagc acaagggttt gcataccgtc cattgatttc catacttatg
     4441 cctgtctata acgttgaaat caaatggctc gaaaaatgca ttgattcggt attggatcaa
     4501 acgtatgatc actgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga
     4561 aagtgtctgg aatcctatca agcaaaggat gatagaatca aagttgtttt ccgacaagaa
     4621 aatggacata tcagtctggc aactaattct gcgttggaaa tggcagaagg agaatttata
     4681 gcgctcttag ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg
     4741 ctaaacatac atccagaact ggatttgatc tacagtgacg aagataaaat cgatgcagat
     4801 ggcaatcgct ttgatccgca cttcaaagca gactggtctc ccgatacatt gatgggaaat
     4861 aattatatct ctcacttagg cgtatatcga acaagtattg tgaaaaagct tggcggtttc
     4921 cgtaaaggct acgaaggctc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc
     4981 cctgcagatc atatttacca tattgaccga gtcttgtatc attggcggac gatcccagga
     5041 tcaaccgcaa gtaatggtga agccaaaagt tatatctatg attctggagt gaaagcattg
     5101 acagatgccc tgtctagaag gaatatcaaa ggaagcgtac gtcctggcag aatttctgga
     5161 ttttatgaga ttgcttatga cgtcttgcaa gaggatttgg tcagcgtgat cattccaacg
     5221 aagaatggct atgaagatct aaaaacttgt gtggattcga ttatcgaaaa aacgtcttat
     5281 ccaaattatg aaatcattat tgcagataat ggaagtacag atcctaaaat gcaggaatta
     5341 tttgcagaat acaagcatca gctgaaagac cgatttatcg ttgaattaat tgacattcca
     5401 ttcaattatt cacgtatcaa caatctggca gcagaaaaag caaacggcaa atacttttta
     5461 ttcttgaata acgatacaga agtgattgaa cctgactgga tgacagcgat ggtttcttat
     5521 gcacagtttg atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg
     5581 cagcatgcag gagtattatt aggaatcggt ggtgttgcag gacatgcatt gaataactat
     5641 gatcgaacac attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc
     5701 actgcagctt gtatgatggt caaggcagct gacttcaatg ctgtcaatgg atttgatgaa
     5761 acattagaag tagcattcaa tgatgttgac ttgtgtttga aagtttatga actgggtcgt
     5821 tacaatgtct atgcacatca agcagaattg tatcatttcg aatctaaatc aagaggttat
     5881 gaagatacac cagaaaaaca aagacgtttt gccggggaaa taaaaaaaat gcaagataag
     5941 tggccttctt atattgctca tgacccattt tataacgata atctaactaa agaaggaatc
     6001 ggcgattttt cattgagacc tgattgatta aaagaactgt attttcattt ttattgatga
     6061 aaatacagtt tttttagtat atgaaaattg taacttgtga ccgaagagat gataggaaga
     6121 acacccattc agtaggaaga atgtgatacg atttttgtcc aactctctgt taggcagaaa
     6181 tatacatata aacatacaat agtcaccttt tctagtttct tttaatttat tttacaattt
     6241 ggttattcac taggttttta aactttgcta tggtaagatg atagaggcat tattttatgg
     6301 ttaggagtgt atatatgaat aaaaatgggg agtggaatgc ggcacgtagg atattcatta
     6361 tcatcatgga tgtacttgta ttgaatgtgt cgatctatct tagctttttg ctgaagttcc
     6421 aaggagaagt cccagcaaga aacttagaga cctttcagca ttcagcagtt tttatttcca
     6481 ttatctttat tgcgttgaat attttgttag gagcttatgt attctatagc cgagtgatca
     6541 gtgatatcgt cttcattact gttattggac aggtgctgat agcattgggg atcatggtag
     6601 ttacttttgc tggacgttgg tttgcatttc cgcgtggcgt catcgtgctc tcgtttatac
     6661 ttggcacgat attacttagc gtttatcgga tagttgttta taaactatac ttaagagtca
     6721 gtcgtgataa gaaagtcctt atcgtaggtt tggagaaaga tgtcgcacca gctatccaaa
     6781 actttcaatc taagaaaaac aacaaacata aagttgaagc agtagtgatc gacaagtatt
     6841 acgaaaacac aaaaaaattg attgatgata tcgatattgt ctacttggct tctaaaatcc
     6901 cagaagacga aaaaatcaat atttatcaat tagtaactaa aaaagagaag aagctatttt
     6961 taaatacaac ttttgaaaat ttaacgatga tcaatccgaa catcatgaat ttcgaagatg
     7021 aaagtatcat tgaagcttcc ggtttcaaaa ttcagccgga atatgaatta ttcaaacgtc
     7081 tttttgatat catcgtttcg cttattttac tgattcttgc ttcacctttt atgctaatca
     7141 cagcgatttt ggtaaaaaca acttcacctg gaccaattat ttataaacaa gtacgtatta
     7201 cgaaaaatca aaaggaattt tcaatctata aatttcgttc catgagtgta acagctgaag
     7261 caaaatcagg accggtcttg gcaaagagca atgatgcacg tgtgacacca gtaggcaaat
     7321 ttatccgtgc cgtacgtttt gacgaattac ctcaaatctt taatgtattg aaaggcgata
     7381 tgtcgatcgt cggaccacgt ccagaaagac catttttcgt tgatcagttc aatgaagaaa
     7441 atccttatta ttatttaaga cacaatgtac gtgcagggat taccggatat gcacaagtat
     7501 acggaaaata tgtatcagat tataatagta aactacgttt tgatttgttg tatatcaaaa
     7561 agtattcctt actattagat ttgaagattt tacttcaaac aatcaaaatc ttatttgata
     7621 agatgtcctc acaaggacta gatgaagatg aaaatatccg ttcgttagca gaaatcgact
     7681 tttcacaaga gaaaatttat cgctagatgg gaaaaatttt gagcaaagaa gtaggagaga
     7741 acattggaaa ataaaaatat aagtatgctg ctttctttat atgataaaga aaaaccagag
     7801 tatttaaagg aagcattaac tagtattttt gagcaaacag taatgccaaa agaaattgta
     7861 ttagtttatg acggccctat aaatgaaaaa cttcaagtag ttgtcactta tttccaacaa
     7921 aaagatcctc acttgtttac agttgtaaaa ttagaaaaaa atcaaggatt ggggattgct
     7981 ttagcaattg gattagaaca tacgaaaaat cgcttagtag ccagaatgga tacagatgat
     8041 atcatggaac cccaacgatt agaaaaacaa ttagagatct tccaaaaaga tccagaaata
     8101 gcgattgttg ggagtaatat tgaggaattt actggcacgc ttagcaatgt tatcggaaga
     8161 aggattgtac ctgaatataa tgatgaaatc cgtgtgtttt cgaaaagaag aaatcccttt
     8221 aatcatatga cagtcatgtt tgataaaaat gctattttag aagtgggaaa ctatcgccca
     8281 ttaattggat ttgaagatta ttatttgtgg gtaagactgt tgaaggcggg atataaaggg
     8341 tataatatcc aagagattct agtccatgct agagcaggtg ctgacatgta tgctagacgt
     8401 ggaggaagta aatatctttt accaggaatt aaaggccgat atcgcgtatg gaaagatggt
     8461 ctaggatcac taaaagattt tctgatagtt atttgcggac atcttgtaat tagtttatta
     8521 ccgaataaaa ttaggggaaa agtatatgaa tcaaaactta gaaaaacaaa ctaaactttc
     8581 tataatcata cctttttaca atgcaaaaaa tactttaaaa agagctgtag acagcgtctt
     8641 aacgcaaggt tatcccaata ttgaaatctt gttaattgat gatggatcta cagatggtag
     8701 tagtgacatt ggtgacgaaa ttgctaatct agagagtaaa gtagcatgtt atcacttgga
     8761 aaacgctggt ccatcagttg ctaggaattg tgggataaaa gaagcaactg gaaatttggt
     8821 aggattttta gatagtgacg attatttttt acctgattgt tttaataatg ttttgaaaga
     8881 ctaccatagg aattaccccg atatcattgc ttttggatta agtaaaggaa aaaatataga
     8941 aaatgcaaat ttatttgttc ccgaaaagaa gattggttgt tcaagtgaag aagcaattga
     9001 gcaaatgttt agaagtaagg cagtggattt ttacgtatgg aataaatttt ttaaaaggga
     9061 attattcaat catatagttt ttccagaaaa taaattatat gaggatatgg ttcctatgta
     9121 tgaagctttt agagcggcta gtaaaattga tattttgcca ttttcaggta ttttttatta
     9181 tcaaaataat gata
//
LOCUS       DAXLPI010000100         9058 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXLPI010000100 DAXLPI010000000
VERSION     DAXLPI010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.101"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(3..827)
                     /locus_tag="ACF3T5_002137"
     CDS             complement(3..827)
                     /locus_tag="ACF3T5_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFD3632702.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(800..1480)
                     /locus_tag="ACF3T5_002138"
     CDS             complement(800..1480)
                     /locus_tag="ACF3T5_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFD3632703.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1503..2441)
                     /locus_tag="ACF3T5_002139"
     CDS             complement(1503..2441)
                     /locus_tag="ACF3T5_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFD3632704.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2686..3537)
                     /gene="ispE"
                     /locus_tag="ACF3T5_002140"
     CDS             complement(2686..3537)
                     /gene="ispE"
                     /locus_tag="ACF3T5_002140"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFD3632705.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3691..6264)
                     /gene="mprF"
                     /locus_tag="ACF3T5_002141"
     CDS             complement(3691..6264)
                     /gene="mprF"
                     /locus_tag="ACF3T5_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFD3632706.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6405..7211
                     /gene="proC"
                     /locus_tag="ACF3T5_002142"
     CDS             6405..7211
                     /gene="proC"
                     /locus_tag="ACF3T5_002142"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFD3632707.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7290..8846)
                     /gene="rny"
                     /locus_tag="ACF3T5_002143"
     CDS             complement(7290..8846)
                     /gene="rny"
                     /locus_tag="ACF3T5_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFD3632708.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
BASE COUNT         2669 a         2008 c         1561 g         2820 t
ORIGIN      
        1 atttagttat tttttcttct gaaggcaacc aatattcgtt tgatgatatt tacaatcagg
       61 aacaaagcaa taaagactaa tgtgattgtt gctcctggag gtgtatctag ataaaacgag
      121 ctagttagtc ctgacagcat ccctagaaat cctacacaaa cactgatagc aatcacgaca
      181 ttgaagcttt tgcctaatcg cataccgatt gctgcgggaa gaaccatgat tgcggatatc
      241 aaaagtgcac cggcaatcgg gatcatcaca gcaatagcca caccagtaat cacattgaac
      301 atcatcgaca tcaaatgaac gggtagccca tccacgtggg cggtatcttc atcaaaagtc
      361 agaacataca tcggccgttt gaataaaaag aacaatatac cgatggcaac gaataagatt
      421 cctaaaaaga tgacttgctc ccatgtaatg gtcacgatcg aaccaaacag ataggattgg
      481 atacttgttg ctgaattccc tccgctcaaa ttcatcaata ctaacgctag cgccaaacca
      541 ccagccatta aaatggcaat ggagatttct gaataggttc ggtagatcgt tcgcagatac
      601 tccaatatca cagctgctaa tacaacgact agcatggtcg taagtgtagg attaaggttc
      661 aaaaagaatc ctaacgctac ccctgctaaa gagacatgag aaagcgtatc agccatcagc
      721 gattgtcttc tgatcactaa aaacacacct agcatcgggg cgattccggc aatgaagata
      781 gcggcaagaa acgctcttct catgaattca tatgaaagca acgccattga gaatcctcct
      841 ttctcaccag tcggatctgt ctgtccgcat actgtttgat atcttcatga tcatgggtga
      901 tcattagaac tgatttccca tggacatgcg cactatgtcg gagaagacga taaaattcat
      961 ttctggattc ttcatccata cctgttgttg gttcatcaag aacaaataag tcagggtcag
     1021 tagcaaaaat acgcgctaaa ctgattcgct gcttttgtcc gccagacaat tcacctattc
     1081 gccgatttcg catatcccac atccctacag cttctaaagc acgctgtaca tgttgatgat
     1141 ctttttcaga gagacgcttg aaccagcggc ctcttggata tcttcctgaa cggactaatt
     1201 cgatcaccgt actaggaaaa cctgcattga aagatgcaac ttgttgagga atataaccga
     1261 tacttaattt ttttccttct tgatttttct cagcaatctt tatcattcct tttgaaggtt
     1321 tcagtaatcc taacgatgct ttcaccaagg ttgacttggc tgctccattt tctccagtta
     1381 aaatgacaaa ttcacccgca tctacatgat aagagacatc ttctagtaca ggttcatcat
     1441 catagtaaaa ggtcaaatca gatatttcga tataacgcac cttacaacct ccattctaaa
     1501 acttaattga tactttcttt taaagctttc agattttttt ccatgacaga aatataattt
     1561 tcgcctgctt tgatttgttc attagtcaag ctttctaaag gattcaacac ttctagcttc
     1621 actcctgttt cattcgacaa tgtctcagct accttcgaag aggcattttc ttcaaaataa
     1681 atcactttga tgttgttttt cttcacaaat tcttttagct cagctaagcg acttggtgtg
     1741 ggttcctgat ctggtgaaac gcccgaaatc gctacttgtt ttaagtcgta ttgattcgct
     1801 aaataagcaa aagcagcatg ctgagtgaca aatgtgcggt ttgttgcatc ttttagctca
     1861 tcggagtatt tttgatccag tgcttcaagc tttttgatat aagcatcggc attcttcgta
     1921 aaagtttcct ttttatctgg atatttttcg cctaattctt cagcaatcgt tctgacttct
     1981 tttatagcta ataccggatc gagccaaaca tgtggatcgt attcatgtga atgaccatgc
     2041 ccttcttctg attcttcatg ttcgtctgct tcttgcgatt ctaaacggtc gattccttta
     2101 gctgcttcga tcatcaaggt ctgtttgcta tccactgaat cctttaacga atccacaaaa
     2161 aattccatgt ctgaactatt atacacgaaa acatctgcat cggaaatttc tgctctttct
     2221 tttgcacttg gttcgaaatc atgcggctcc gttccagcag ggatcaaaag tttcacgttt
     2281 ccttcatccc ctacgatctc ttttgtaaaa tcatacatcg gataaaaagt cgtaacgatc
     2341 gtcaaatcct cttttttgtc tgcttccttg ttcgtatttc cacatgctgc aaacagtaaa
     2401 gcacctatca ttaccgtagc tgtcaaaaga tattttttca tttatatgct cctttcaatg
     2461 aacaaatcgt aattttacga tttattaacg tgaactaatg tatcaaaata aaaaaacaca
     2521 gtcaatagaa atccatcaat ttctgattta agcatgtata ttcttgatta aatcgtaaaa
     2581 tggatgagtt gctgctcata tctagccatt tcactacaat aaaaaactga agaataccat
     2641 tttaggattc ctcagttttt catcacctat tttacttctt atattttatt gcagggttcg
     2701 tactaaataa acttcctcac aaaaaccttt taacccatta tatactctct gcgctcttga
     2761 ataatgttga cacaaggcaa aaacggttgg tccgcttcca ctcatcaatg cagcatctgc
     2821 tccgtacttg atcatacgct ctttgatttg atggatgact ggatgccgct ttcccgttat
     2881 atcttctaaa ctgtttccca tttttttgat catctgctga taatcttgct ctcggatggc
     2941 ttcagcaagt ccgtggatgt cttgatgttg gatggcttgc gtatctactt ctctaaacac
     3001 tgtacgtgta gagacactca ttcttggttt gaccaacaca acccagcact gtggcataga
     3061 aggtaatact tggatttctt cccctttgcc accgacaaat gccgtacttc cgtagacaca
     3121 gtaaggaaca tctgtaccga cttccaaacc gatttctgcc agttcttcta acgaacagcc
     3181 cagattccat aattggttga tccctcttag agcagctgca cagtctgtac ttcctccgcc
     3241 taatccagct gctactggaa tattcttctt aacaaagatc ttgacaccgg ttttgatatt
     3301 gtatttattt tttagcaata aagcagcttg ataaacattg ttccgttcat ctaccggtaa
     3361 aaatgcttta tttgtttcta caatgatttt gtcttctgtt atttcttcaa aaaatagatg
     3421 atctgccaag tctacgctcg acatcaccat ttccaactca tgatacccat cttgccgtct
     3481 atataataca tccagtccta aatttatctt ggctggcgct ttttcaatca attccatcct
     3541 ctcacccatt cgaaaagtct aagtcgcaag tattctatca tagggaaaaa taaaagtata
     3601 gcttatgtct gcctagtggt tgattttgtc atcgtttcaa cagctttcac agaattttct
     3661 cttcataatc caaacaagtt ctagtgattc ttataattct gtgagcactt cttctgccaa
     3721 actcggaaca tttccttttt cttttcctat cagactgagt agctgcatgg tagcaaatcc
     3781 gaaattactt cctttcctat aagcaaaata acgtccttcc caatgatcga catattttaa
     3841 tttcttttca taagcagact ggaaggaata gaaaaaatta ccataattat atacgatatg
     3901 catgactttt tcttgaaaga aagaaaaatc tgtttctcca acattagcta atggagctaa
     3961 gcctagattg gcttccgagt agcccatttt ctcgtataca ataaacagat tggctaacag
     4021 atagtcagct aattcctcag attctttagg taagacccgc aacaaatcat atcccgcctg
     4081 cttcttcgtc atcggctgag cggtgatgaa gccaacgact ttttgatctt tacggacaat
     4141 accgacaccg ctagataata catatgattt atcaaatcgt cccacagaga agtatttttc
     4201 tcgtgcacct tgaagccatt tttgtgaaac ttcatttaca gcttgatgta attcttccgg
     4261 cagttgatcg taccaagtaa aatggtatcc ttctttcatc aaacgttgat attcaagttg
     4321 ctgactcagc cgagacaagt cttctgttgc tagatcaaca atccctgctt cacctacttt
     4381 cgtgaagtga aaacccaatt catgaagcat cactacgtag ctttctgata tcttataaaa
     4441 cgcaagctga tagcctaaac aatctgcttg tttcataaac tgattcgttg ctgcccgcat
     4501 cattttagga ttgccaactg gatctcctaa aacaaagcat ttgtttcctt tgatctgata
     4561 accaaaaacg acttgatctt ggtcgtcttc ttggtaataa aaataactgt aacctgacaa
     4621 gcgaagctga tggcttgtag ctgtgcctcc gtatgtttcc aataaacggt caaaacggtc
     4681 tttttcgtac tcactgccaa gcttcgtagt atcttcagct aaatattgat aaagcgccac
     4741 taaagctagc aatgagatgc ccaaaccaat aagccctgag aaccacacat catctgaagg
     4801 aaacaaaata aaatgatttc ctgttattcg accgactaaa aagcgtccgc tgtgatagcc
     4861 tgcaattgcg taaacgataa acaaaaagcc gaacaagcta gcatcaaaga ttatagctcc
     4921 ccaagaataa acgaattttg ttcgataaaa ttcttttctg gataaccaca cagctaacag
     4981 aatcaagaca tataccaata tcaactgcca agataacgta cgtgtgattg tatttaaaat
     5041 accaaaagat agtaaagcaa tcgtaggcag aaatgccttt ttgactttta ctgaaagtgc
     5101 tctagcaagg ccgagtaata aaaagccaac caatagattc actgtctgat ctaaaaaatt
     5161 gaatgagaac ggcaataata cttgaaatag ccgactgatt gcagacagat tagtgattgt
     5221 tgatagaagt accatcatga tccccgcaaa atagacagca cttacgagca cgatatgcgc
     5281 aaccttttgg aaaaacagtc gcggaatgtt atctaaaaat cgattgattt tgactcctgt
     5341 atgtgtgatg aacaatagaa gtcccgtcat aaaaggtaga acataataaa acaaccgata
     5401 atataatagc caaactactg ctacatcttg ccgaacagac aattgactca ttcctaagat
     5461 catcaaaacg tcaaaagtcc ccattccacc tgggaccatc gtcagcattc ctatcaatgt
     5521 agcaatcatg aacataggat agatcgaaag catcgaaata tctattctca tcaacttgcc
     5581 gatgatcaaa aataccgcaa gggctcccgt ccattgtcca aaggatgcca gtagcagacc
     5641 aagcaagcct tttggataaa actcgaagaa caatgtcttt ctctttagat aagcaaaaaa
     5701 gaaaatgact ggtgctaaaa aactaccgcc gaaaagccag atccaatagc ttcgaaaaat
     5761 actttctgac tgaataaaaa agacatcaat aaatgtaaga aacgaccata cagacaaacc
     5821 agtcagcata aatagtgcga cttttgatac agtggctaaa accatttttc tatttgtctg
     5881 ctttccataa aaactagccc gcagacttgc tcctaccaca ccaccaaaac cggcgagatt
     5941 gttgatcgtg tttgttaccc acgcagcaag aagaaactct tttctttcca tttttggttt
     6001 tccttgtttt tctaagactc tgatcgtcac ccaatcatac aacaacatcg gagtgacacc
     6061 cagcagtcca gccagaatca tgagcaggat cgtcatgcgg ctttgttgtc ccatcctttg
     6121 ccaaatatct tgccaactca tgccattagc aatatttgcg acttgatttg ccacaaagac
     6181 taacacggag ccgaaaaaaa tcagttttaa aatcagacta tgttccttta tccattgcag
     6241 catcaagtat cgtttatttt tcacattttc ccattcctaa cttgtatact tttgtgtcat
     6301 accccaatgt tatcttattt attttaattt ttccacttta gacctgttct ttgcttgcga
     6361 tgatagaaaa agaaagatat cctaatgcta aaggagagga taacatgaaa gtaggattta
     6421 tcggcacagg gaatatggca caagctatta ttgatggttt attaaaaaaa ggaacagtct
     6481 cgaaagaaga ggtgttcgtt tccagtggac attttgaaaa tgcacaaaaa tttgctgctg
     6541 aaactggcgt ccatgcatgc aaaagtaatc aggaagttgc atcacaatca gatatcgttc
     6601 ttttagccgt gaaaccaaaa atcattgaaa aagtattgga agaactaaga gaatatagcc
     6661 agaaagtctt attcatttct attgcttcag gaaaatcatt aatagattta gaaacagcgc
     6721 ttgaagatcc tcaggcctcc atcgtacgtg tgatgccgaa tgtcaatgtc agcgtaggag
     6781 caggcgtttc tgcgatttgc aaaaatgaag ctgccacgga ggaacagctt cagcaagcta
     6841 cgatgatttt tgaagcaatc ggcagcgtct atcctttagc agaatcagat ttcagtacat
     6901 tcattggcct tgctggcagc tcacccgcat tcgtttacat gctgatcgat gcgatgggac
     6961 ggactggtgt actgaatggt ttgtccaaac agacagccac tgagattgct gcaaaagcaa
     7021 ttatgggttc ttgtcaaaaa ttcctttcaa gtgataaaaa tccatgggaa cttgtagacc
     7081 aagtttcttc tcctggcggt acaactgttg caggtgtagt tgccttagaa gaagcaggat
     7141 tcatcccagc aatcatcaaa ggaatcaata cgacgatcga gaaagatctt gagatgttga
     7201 ataaaaaata atgatagcaa tgttttttcg tgccttacaa aaaaagaaga gctgtggctt
     7261 ttgccgcggc tcttcttttg tattttttat tatttagcat aatcagttgc acgagtttcc
     7321 cgaataacga ttactttgat gtgtccagga taatccagtt cttcttcgat tcgtttacga
     7381 atatcacgta ctaaacgaac agcgtctaaa tccgagattt cttctggctt gaccattacc
     7441 cgaacttcgc gtccagcttg aactgcgtaa cttgattcta caccggcgaa gctgttcgaa
     7501 atattttcca gacttcttaa acgacggatg tagttttcta atgattcact tcgcgcacca
     7561 ggtcttgctg ctgagagcgc atctgctgca gctacaagga cagagataac agaagtagct
     7621 tccacatctc catgatgaga agcgatcgcg ttgattacta ctgggttttc tttgtacttg
     7681 gcagctaatt ccgcaccgat ctcaacgtga gatccttcaa tctcatggtc aagtgctttc
     7741 ccaatatcat ggagcaaccc ggcacgtttt gctaattgga tatcttcgcc aagctctgct
     7801 gctaagatac cagctaattt tgcaacctcg accgaatgct tcaagacgtt ttgtccataa
     7861 ctagttctga atcgcagacg tccgatgatc ttgattagat ctggatgaag tgtatgagca
     7921 ccgacttcaa acgctgcctc ttccccgtat tcgcgaatac gttcatccat ttctttacga
     7981 gatttctcga ccatttcttc gatacgcgct ggatggattc gtccatcttg tatcagtttt
     8041 tccaaggtca tccgtgcgat ttctcttcgg attggatcga atccggaaag aacaactgct
     8101 tccggtgtat catcgatgat taaatcgata ccagtcagcg tctcaagcgt tcgaatatta
     8161 cgaccttctc gtccaatgat acgacctttc atttcatcat tcggaagggt aacgactgaa
     8221 acagtcgttt ctgaaacttg atctgctgcg cagcgttgaa tcgctaatga taataagttt
     8281 ttcgctttgc gatctgcttc ttctttggca cgctgttcag attcttttac cattagagtt
     8341 aactcatgat tcagctcttc ttcagtcgat ttcatgatga catctcgtgc gtcctctttt
     8401 gataaaccag caattttttc tagttctttt tgttgatctt caatcatttt ttcaacgtct
     8461 ttttctcgct cttcaattaa ttgctgtttt gaactaagtg cttcttcttt actttcaagt
     8521 gagttctcgc gtttttctaa cgagtcatct ttacgattca gcgtttgttc gcgttgaatc
     8581 aaacgattct cttgagattt taattctaac ttactttctc tcagctcact ttcaacttca
     8641 gaacggtatt tctgattttc ttccttagct tctaacaatg cttctttttt tagagtttct
     8701 gcttcttttt ttgcactatc gatgattcca gcagctgatg attgtgcacc agctatttct
     8761 ttctcgtgac gtgactttgc gaccataaac cctaaaccaa gaccgacaat taaaccgatg
     8821 atagcgagga gaatgttgaa taccataaaa tccacctcca tactatcttt ttcattttta
     8881 tcatctatac agcttctatg ataaattatt ctaaactcgt caatatgcat cttcgcatat
     8941 atgtgtacaa aaaacatgca caactttatt ctaatgtttg taccccgtag tgtcaacttt
     9001 aaataaacaa agaaacggct ttctacttat tcattaaacg atggttttta aaataaat
//
LOCUS       DAXLPI010000101         8944 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXLPI010000101 DAXLPI010000000
VERSION     DAXLPI010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.102"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(15..1154)
                     /locus_tag="ACF3T5_002144"
     CDS             complement(15..1154)
                     /locus_tag="ACF3T5_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFD3632709.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACF3T5_002145"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="ACF3T5_002145"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFD3632710.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACF3T5_002146"
     CDS             complement(2967..3722)
                     /gene="tpiA"
                     /locus_tag="ACF3T5_002146"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFD3632711.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACF3T5_002147"
     CDS             complement(3826..5016)
                     /gene="pgk"
                     /locus_tag="ACF3T5_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFD3632712.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACF3T5_002148"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="ACF3T5_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFD3632713.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="ACF3T5_002149"
     CDS             complement(6168..7259)
                     /locus_tag="ACF3T5_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFD3632714.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACF3T5_002150"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="ACF3T5_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFD3632715.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="ACF3T5_002151"
     tRNA            8802..8873
                     /locus_tag="ACF3T5_002151"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
BASE COUNT         2318 a         1897 c         1648 g         3081 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgcccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag ttttgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc agacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaagtctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aaag
//
LOCUS       DAXLPI010000102         8888 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXLPI010000102 DAXLPI010000000
VERSION     DAXLPI010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.103"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(154..552)
                     /locus_tag="ACF3T5_002152"
     CDS             complement(154..552)
                     /locus_tag="ACF3T5_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFD3632716.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(549..1322)
                     /locus_tag="ACF3T5_002153"
     CDS             complement(549..1322)
                     /locus_tag="ACF3T5_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFD3632717.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1319..2002)
                     /locus_tag="ACF3T5_002154"
     CDS             complement(1319..2002)
                     /locus_tag="ACF3T5_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFD3632718.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2169..3056)
                     /locus_tag="ACF3T5_002155"
     CDS             complement(2169..3056)
                     /locus_tag="ACF3T5_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632719.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3053..3922)
                     /locus_tag="ACF3T5_002156"
     CDS             complement(3053..3922)
                     /locus_tag="ACF3T5_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632720.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4059..5345)
                     /locus_tag="ACF3T5_002157"
     CDS             complement(4059..5345)
                     /locus_tag="ACF3T5_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3632721.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5618..6196
                     /locus_tag="ACF3T5_002158"
     CDS             5618..6196
                     /locus_tag="ACF3T5_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFD3632722.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6196..7125
                     /locus_tag="ACF3T5_002159"
     CDS             6196..7125
                     /locus_tag="ACF3T5_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632723.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7122..8858
                     /locus_tag="ACF3T5_002160"
     CDS             7122..8858
                     /locus_tag="ACF3T5_002160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD3632724.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
BASE COUNT         2825 a         1881 c         1501 g         2681 t
ORIGIN      
        1 tagcatattt tgaaaagaaa gtctgatgct tagagtagaa tttcataaaa atttactatt
       61 tcataagtat tcacgagaaa aatacaaaca aaaaagcgga aataacgctc atccgccttc
      121 ttgtttgtgt ttatttcctc ttcaaacgct tgtctatgat tcagactttt ctctatcgtt
      181 attttcatct aaaagttctt gcattccgat aatttctcga tattgatgaa tcttagtgat
      241 accaatgact ttgtctacat caatactaaa acaaatacca ttacctggaa tatcgatgtt
      301 gatgccttga ccaatcgcat ccattacttt attcgtcaaa ctatcgggta ctaatgtcaa
      361 cacaatatct ttttctggcg aaatgtccaa acctaaaaat ttccctgaat catgtacacc
      421 agaacctctt ccatgtaaga tcgttccgcc tgctgcacct gcttcttttg aaaagtcgat
      481 aacatcttcg cccattcctc cgtcaacgat cgtcacaatc atgtgaagat tcaatgctac
      541 aagatctttc atttgctttc ccccaatttt tgcaatcttt ctttttcgat tctgtctttt
      601 tcatcttttc gtttataaat atatcctagt actaaagtgg ataatattgg tgtcaatgca
      661 atcattgcaa ccacaccaaa cccgtcaatg atcggatctc ttccttcaat ttgtgcagcg
      721 attgccacgc tcattgataa aataaatgtc gaacacatcg ggcccgttgc tactccaccg
      781 ttatcaaaag ccattgctaa aaagatcttg tccacttttc gtcctaaaat aaatgctagc
      841 ccgtatcctg ggatcaagaa ataataaaga gagaatccga cgagcaaacg ccacatagac
      901 aatcctactg ctgcgccgat cccaattgaa attaccatca gcatgatttt cgatcggatt
      961 ctcccctcgc tcaattcttc aacctgtttg atcattacat gaatcgccgg ctcagcaaac
     1021 cctactaaga accccattaa aaagccaagg ggaaccagta tataattgta aggcaatccc
     1081 gcaatagcac tgcctaaatg ctgtcctacc ggtacataag ccatattgac tccgtgcagg
     1141 aaaaggatca acccaatagt cgttatcaag aaacctttca taatgtcaaa gaaacgcttt
     1201 ttgcgcatac gaaaactaat gatattcatt aaaacaaata ggattacgat aggtgccact
     1261 gccaaaatga cttccatggt aacgatttcg attccatcta tcacttgctg ccagaaactc
     1321 attggaagat cacccccaaa atcatcacgg caagaatcgg ccctaacgaa gcaataccga
     1381 ccattccaaa actgtcattt tcatttttct tttgtttgat catactagtc atccctccgg
     1441 caagggccaa gataaatgga acagttactg gaccagtagt cactcccccc gaatcaaaag
     1501 caatcggcat aaattcggaa ctggtgaaaa atgaggcgat caaaacgcct atgtagccta
     1561 tagccatcaa ttgataatag gatagcttga atacgacccg aagcagcgca aaggctagaa
     1621 atactccagt gccgacgctg acgatcccaa tcagcaacac tctgccaatc tctccgtcag
     1681 atatatcata tacttgttgt cccaaaactt gcacggaagg ttcagcaatc gtgatcacga
     1741 tcccgatagc aaatcccaaa ctgaccaaaa cggtcaaact tttctttttg atcatatatt
     1801 tccctactag atctccgact tccatcatag aataatctgc tccaaacaaa aagagcgtca
     1861 ttccgatcat catgattgcc gcaccaacca gaaatgaagc gaggtcttct ccttctaacg
     1921 gtgcaaaaac aaacgttaaa atgacaatca ggacagtcat cggcaatata gcagcaatca
     1981 cttctttaaa attttttagc atgtatcctc ctcctttact ctttaagttt accttaaaat
     2041 gaaagagttt tcgagcacaa acttaaaacg ttatcattta atttttctca acataaaata
     2101 acacacgtcc caaaagtatc tttttacttg gaaaaatgac tttgaaggaa cgcgcttatt
     2161 gtgttttttt attttacaga gccgatcatt ccttgaacga attgcttttg caaaacaaaa
     2221 aatacgatgg cagttggtaa agtagcgatg actaatccag ccatgatcat tccatagtct
     2281 ggtgtatagg aagcacccat agcagataaa acaagcggaa ccgtccgttt atcaggagat
     2341 tgcaaagcta ctaagggcca taaataattg ttccagctac tcatgaatgt gatgattgca
     2401 gccgcggcat aggtattttt agccgtcggc atatacactc taaaaaaaat acttagttct
     2461 tttagaccgt ctaatcgagc ggcttctact agatcttttg gaaaagattt aacattttgt
     2521 cgaaagaaga agatcaaaaa agcagtgcta acagatggca ggatgacaac caagaaactg
     2581 ttgattccca aaaccgttcc gttcaattgt gaaaacattc gatataaagg gatcatcaat
     2641 gccgcaaaag gaaccatcat cgatagcagt agaatattga aaattaggtc ttttcgttta
     2701 ctttgataaa tctcaaatcc atatcctgcc attgacgaaa tcagcaatgc aaacacagtg
     2761 gtcaccaaag cgatcatcgc tgaattccaa agggaacgag taaaattcag ctcattggaa
     2821 aacaaatttt gaaaattgat gatcaagtta ctgccaatct ttatttttcc cgatgtgata
     2881 tccacgggtg tattcgtcat tcccaaaacc atccagacaa aagggaaaat ggaaataaat
     2941 gataggatgc ttaatgctgt atactttaat actgacccaa ttgtcgaacg aagttctaat
     3001 gcttgttttt tgacgttggg gcgttcgatt gtttcttttt ctaccatatt tttcatcgac
     3061 tttctcctcc tactttcgtt tggattatcg acaatatcac aataaaggaa acaataacaa
     3121 aggaaactgc tgccgcatat ccaaaattag gcgtgaattt ataactcaaa ttatagatgt
     3181 attgagatag ggtggtcgtt gcatttgctg gtcccccgcc agtaatattt tgtacttcat
     3241 caaaaagctg caatgtcccg atcgtcgaag taatggaagt aaacaaaatg attggtttta
     3301 aatttgggat cgtcactttg ataaactgct gccatttaga agcaccgtca atagatgctg
     3361 cttcataaat ctccgaatca atattttgca tgcctgaaag gaaaaaaatc atattgtatc
     3421 cagtccaacg ccaagtgatc gaaagaatga tcagaactct cgcccaaatt ggatgagtca
     3481 accagccaat cggtgtgtca atcataccaa tgcccaacaa caagttgttt acaaggccag
     3541 attgagaaaa tagacttttc atgatcagag aataactgac catcgaagtg atacatggaa
     3601 gaaagatagc ggttcgaaac aaccctttat atttcaactt tttatcattt aaaaggttgg
     3661 aaataaaaag tgctagaaac aacatgatcg gtacttgtac aagtaaatac aggaatgtat
     3721 taaaaaaagc ttttttaaaa gttgcatctg tcaacatccg ctgataatta cctaacccgt
     3781 taaatgtcat attgacaggg ggaccacttt gaaaagacgt gatcaaagcc aaaaccatcg
     3841 gaataaaaac catgattgat atcaaaatga ctggcaagat taaaaaagca tgcccgtttt
     3901 tgaataaatc tttctgcttc attcttttcc ctccaattct atcattgatt atctacatga
     3961 aaagacaaga aagacaatat ctgtaaggca gacgtgatct tttttgtctt ttcgtttgtc
     4021 tgatgaaaaa gcgaagaaat tcactttttt accaccgatc aattagccaa ttgtgcttct
     4081 acttgttttt gtgtatctgc tagtaccttg tctgtctctt ctccattgat gattcgatga
     4141 agagcttcag ctacgactgc ttcaattgca taggtctcat gtccgtaatt cacttctggt
     4201 atttcagatg tccatgttga aaaatcgtca aagatctttt gaccactgta aaattcagaa
     4261 gcttcttgat aagcttcttg ttcattcgcg ctaagcatcg tagatactag tccgatttct
     4321 ttcgcaagag tgcccatcag ttcttcattc gaagcaaacg ttgcttctag gaaatctttc
     4381 gctgaatctt ctccagctac tcctttgatc acataccagc cagcgccgcc aaggttagaa
     4441 gcttgcgctt tttgcagatt ttctggaagt gcaggtattg gtgcaatctt ccattttcca
     4501 gattggtttt ctgctccttg gatcgttgaa gaataccaag cgccagttgg gctagaagct
     4561 actgctcctg attgtatcgc agttactcca gcattccagt cagaggtttg ttcgactaga
     4621 tcttctttca aaagagtggc aaacaattca agaccatact ttaaagatgc attgtcttgg
     4681 atcgtgactg tctccccatc ttcagatgta taccattctc ctgcctgttg cataatcatc
     4741 cttactcgac caaggtcaga aggattgact tcagcgattt tctttcccgt cttttctttg
     4801 acatctcgcg ctacttgaat atactcgtcc cacttcagat tctccatatc ctcttctgta
     4861 taacctgctt ctgccatcag atccgtacga tagaagtttg ctgttacgcc cgaatcgaaa
     4921 ggaacaccgt atattttatc tcctactttg tttaccgcaa atttatacgg tgcaaaatga
     4981 tcttccttta caatagcaga caaattagcg aatgcatctg gataagaagt caaatagcct
     5041 tgtatcctat aatcttcaat caagacgata ttgggcaatc cttctgtatt cccgctcgct
     5101 aaagcggtgt tcagtttctg aacgatatca tcctgagaca tcgtaacaac ttctatctct
     5161 gtttcttcgt tctcataaac tttttttgct tcattcactg ctttgatatt gaatgtctca
     5221 tcccacgccc agacagtgac tttttcttct ttgtctgctg tactactagc acttccgcat
     5281 cctgttaaga aacctgatgc tgccgctaaa caaacgactc cagcaattaa ctttctgatg
     5341 ttcattttta tccgctcctt ttccatccat tagttgttta cgtttgctac attcatcata
     5401 ccaaaatttg aaaacggttt ctttattatt cagatacttc aaaaatttct cctaaaataa
     5461 taaaaaaata caaatcaact atgacaaaag gaataatctt agcatctaga ccaaagatcg
     5521 aaatggtgag gtttctttaa aataaaaata tccttgcatt ttctcctaga aataagtatt
     5581 tttaaattac aagactattc ttaaggcggg taaaaaaatg ccaatacgcg taattcttat
     5641 cgatgatgaa cagacgattt tagatggaat gaaacagttg atcaattggt ctgagcacgg
     5701 gtttcaatta atagggactt tttccagtcc gctgaaagcg cttgattttt ctttggtaca
     5761 tgcaatcgat atcgtggtca cagatgtaat gatgcctgaa ttaaatggaa tcgaattatc
     5821 gcggctatta aaacagcaac aacctgaaac acagatcctt atcctctcta gctatgatga
     5881 atttgatatg gttaaagatt cttttaaaga aggcgtcgct gattatcttt taaagcctcg
     5941 attgagtcct gatttttttc taaattcctt aactgccgtt tcacaaaaaa tcaaaaaaaa
     6001 gcccaacaaa agcccaatca ccaatcgtta tgagcagatc tgcgagaatc tttcacagca
     6061 gattgctgga caaactcgtc cattgcttga ccctcttctt tttgaacaca ccttgttttt
     6121 ctgttatata ccgaaaaacg gccattcagt tcaaaacaag gctctaaaca aaaacagtcg
     6181 atcacccatt cattgatgga tagcgacgat tcgtttacta tttatcctgt ttctaccttt
     6241 tcggaagaat ccggctatgt aatcaatact gattccaaag atacacttag gaagtttgtt
     6301 caaaagtttc gtcaagaagt ttttccaggt gatttttttg ttgtttctga ttcttttgca
     6361 atccaagata tcaatgaaat attccaagag atcagaaatt gcaccaaagg acaacgtttc
     6421 tatcataagg gaaagaacct tgcttatcag catgaacttc ttccgttatt aaccaaccac
     6481 gagcagcaag caagtagtta tttaacaaaa atcgttcata aagactacgc tttagctgtc
     6541 aaagaacttg agaatttctt cacgacgata ttggaacagc ctattgatcc agcttatttg
     6601 aaacatgagg caatcaatcg tttctatgca ttactcaacg ctttggctga cgagcatgtt
     6661 gggaatgaag aactttcttt gctcaagatc gcccttcctt ctttattagc cgatgctgag
     6721 tatttcgaag atttttctat tcttgtcaaa gaaacacttg agaaactcac ccacttttct
     6781 gcaaatgcaa aaaaagacaa tttcgatatc ttgactttga tctacgagtt cgtccaggaa
     6841 aattatgaga aagaaatcaa tttacaactt ttatccgaaa aatatcattt ttcatatagc
     6901 tatctctcag ccatctttac tgaaaaattt ggtatcaact tttcaaaata tctaaaaaaa
     6961 gtccgtatca gtaaagccaa gctctttttg acggaaacac ggcttacttt gacggaaatc
     7021 tgtgagaaag tcggctatac cgaactgggt tatttctcca gagtttttaa agaagagaca
     7081 ggaatgacac cttctcaata ccgtaaagga acttttgtaa aatgagaaaa aaaatcaaag
     7141 agcatgaact ctttgccaaa cttctgttca ttttgttcat cggtcttttt ctgcaagcca
     7201 tcgtcatttc tttgtttatt tatcatcgat caagggatgc ctatatccag ctgttcaatc
     7261 aatcgaatga tgtggtacta aaaaagattc aatctgaatt tgaaagtttg aatgatacaa
     7321 tcgagaatac attagccgct tttgattcta atccagctgt caaaagctat ttttccaatg
     7381 atccagccca acatatggaa cagcttcagc aactaagaac aatccaaaaa atgaatgact
     7441 ctctttcaaa aatccatcca atgatcgatt acgatgtatt gatctttgga gaaaatggaa
     7501 gaacttttgt cggaaacgac atgctcactg ccgtatctgc cgattctttt tttcaatctg
     7561 cgattgctca gagagtgaat gaacgtgctg ctgatacaca aatgcttttt gcacaccacg
     7621 gattgactct tcgcgataag aaagcaccca gcgtcttctt tgtcaaaaaa ctgaaaaata
     7681 cattgaatca cgtttacgga tatgctgttt tatcgatctc atccaagcaa ctagcgaatc
     7741 tttttcaatc tgtagtaaat cctgagatat cgaagatctc cttcatgaac gaagaacaga
     7801 cgatcatcgc atcaaatgaa gaaaatacaa ttgggagccg atcaatgatt ttcaaaaaac
     7861 taacagtagg tgaaacaacg atttcaaatg gacagcagtt gacccgtctt cctctgtacc
     7921 gccaggattg tacattactc agtcaagtcg atcttcattc tttatctaac caaatgggcg
     7981 tgatttttcc gattattttg tataatatcg tgacacttgc ttttgctggc tcattggtat
     8041 ttatgtattt ggatcgacac acaaaatcga tctaccgctt gattgattcg ctaaaacggc
     8101 tagaggaaga tggcttaaaa gctactgttc ctgtacaagg aagccatgaa gtccaattgc
     8161 tagggaatac gatcaatcaa ttgctaagta atgctcagca tcattatgaa cagacgatcc
     8221 aagacgaaaa gaagaaacgt tcgcttgaaa tccaaactat gcaagcacag atacagcccc
     8281 attttattta taatacgctg actagcttga aatttttgat ttggcagaaa aaaacagatg
     8341 aagcagttca aggaatcgac agtttcatta ctttactaag aagtacgatt gggaaaaaag
     8401 aagaagtgat tccagtcaag gaagaaatca aaagcgtaca aagttacatc gatcttttga
     8461 gtatgcgata tggcgaaaga atctcggcta aaataatgat tcctgatgaa ctgcagtcat
     8521 tatctatgcc gaatatgatc cttcaaccaa ttatcgaaaa cgcctatctt catgcttttc
     8581 acaataaaac gcaaggattc atcacggtgt acggcaaaac aaaaaatcag cttcttttat
     8641 tcgaagtgat tgataacggc gatggcttcg atactgaaaa taccaaacaa aaaaatgatc
     8701 atttttcagg aatcggcatc agccatgtag atgagcggat acgcctaatg tatggcgatg
     8761 catatggctt aacgatccaa tcagtgattg ggacaggaac gactgtaacg atttgtctac
     8821 cgcttcttcc taaaaatcaa tcagaatatc aaaaataaag aatagccaag cgctgatttt
     8881 tttatttt
//
LOCUS       DAXLPI010000103         8686 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXLPI010000103 DAXLPI010000000
VERSION     DAXLPI010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.104"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..428
                     /locus_tag="ACF3T5_002161"
     CDS             <1..428
                     /locus_tag="ACF3T5_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632725.1"
                     /translation="RYSKNKKAEKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFME
                     RSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTY
                     LYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFYRNKL"
     gene            complement(554..1231)
                     /gene="nth"
                     /locus_tag="ACF3T5_002162"
     CDS             complement(554..1231)
                     /gene="nth"
                     /locus_tag="ACF3T5_002162"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFD3632726.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1238..1936)
                     /locus_tag="ACF3T5_002163"
     CDS             complement(1238..1936)
                     /locus_tag="ACF3T5_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFD3632727.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2204..3388
                     /locus_tag="ACF3T5_002164"
     CDS             2204..3388
                     /locus_tag="ACF3T5_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFD3632728.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3394..3837)
                     /locus_tag="ACF3T5_002165"
     CDS             complement(3394..3837)
                     /locus_tag="ACF3T5_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFD3632729.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4063..4350)
                     /locus_tag="ACF3T5_002166"
     CDS             complement(4063..4350)
                     /locus_tag="ACF3T5_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFD3632730.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4521..5873)
                     /locus_tag="ACF3T5_002167"
     CDS             complement(4521..5873)
                     /locus_tag="ACF3T5_002167"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFD3632731.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSDPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQEALGEIETAQ
                     "
     gene            complement(6145..6591)
                     /locus_tag="ACF3T5_002168"
     CDS             complement(6145..6591)
                     /locus_tag="ACF3T5_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFD3632732.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6827..7753)
                     /gene="cysK"
                     /locus_tag="ACF3T5_002169"
     CDS             complement(6827..7753)
                     /gene="cysK"
                     /locus_tag="ACF3T5_002169"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFD3632733.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7893..8686)
                     /locus_tag="ACF3T5_002170"
                     /pseudo
     CDS             complement(7893..8686)
                     /locus_tag="ACF3T5_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
BASE COUNT         2366 a         1770 c         1435 g         3115 t
ORIGIN      
        1 ctcgttattc caagaataaa aaagctgaaa aattttgttt attattaaat aaatttactt
       61 taattgctcc tatccttgca tttattattt tttctgtact tttaagcact actcttaaag
      121 gaaaatttat ggaaagaagc tctcatgcaa tgattctcac ttttttatgg ttgttgttca
      181 ccagaatcta tatttttctg atgagcctga aaccgcccaa atccattagt ttatgtttag
      241 ttataaatgg tattttttta ttatcactta tcatttttat tacaccttta gatagatacg
      301 tcacttattt atataatcct ttggaatact ggacttattt tataggtata ttggaaggca
      361 ttattttcta catcgggtat tttcccaata aaaataacaa cttttatttt tatcgcaata
      421 aattgtaata agttaccatc ctagctactg agtatgtaca tttacttttt catgcaaaaa
      481 agataaacga aacagctttt ctccataata acaagataat tcatacattt atttgcaatt
      541 aaagaaacca cgtctacagg cgtggtttct tttttagcgc cttttctttt agacgcatcc
      601 gatttttccc atcttgacat attgatagca aaggacatac ctcgcacttg ggatttcttg
      661 ccgtgcaatg atatctacca aaaaaaatca gtgtatgatg tgtctttacc cataattctt
      721 gtggtacttt ccgcatcagt gtttcttcta cttccatgac cgtagcatct agtttgcaga
      781 tcctcaggcg ttttgagaca cgttccacgt gggtatctac tgcgattgct ggtatcccga
      841 atgcatcccc caatacgaca tttgctgtct ttctgcctac accaggaaga gacatcaatt
      901 cttctcttga cgttggcact tgcccatcga aacgttcaat cagctgctgt gcacacgctt
      961 tgatgttttt tgctttattt cgatacaaac caatcgtttt tattttaaga atgatttcat
     1021 cgattgaagc ttctgctaac gcatcgggtg tcggaaaaga ggcaaacaaa tctggcgttg
     1081 ctttattcac tgatacatct gtcgcctggg cacttaagat cactgcaatc aataactcaa
     1141 atggattatt gtgcttgagt tctccatgtg cttcgggaaa cattccatac atcgtttcta
     1201 gagcttccat tgttttttgt ttgcttaaca tattccgcta ctcctccaac caatttttca
     1261 gtgagacttt cggcaattct tcttgttgag ctgactgttc tacttctctt tgaagaagct
     1321 gctgttttct tcgtttttgg tcttcttcca cttgttgttt tgtacgtaga ttttttcttt
     1381 cccacgatag caaaatgcgg tcaacgtaat taaaactata agcctgattt aatacggctt
     1441 cccgcagtgc tagcttcaaa atttccggct gataatggtc ttcttctagc cattgtccga
     1501 tccgctggaa ctcgatcgaa gaaagcgggc gtccaaattc ttgttcaaat aattgataca
     1561 ctgactggat ttctttttct tgtgtttttt cttcgtattt tttttcttgt gtttttaaat
     1621 actcacctaa tagatcatag atggggtaaa gattatatcg atctgctttt ttaccgttga
     1681 caatcgtcgt ttctatcttg ataaatccat ttgttacgag acgatccagt atttgaaaaa
     1741 tctgatcttg tttgattccc atatccatag caatcatctg caaatcaggg aaagtatctc
     1801 cttcaagctg tgccatatgc aactgtagaa caaataaaaa ttcttcagat tgtagaccga
     1861 tcctatgata atttttaagt aaaagattag atatgactgt ttgtccagcg cttaagtaat
     1921 tttctaaatt caacatgatt tctcctccgc tcaatcacag tatacgtaaa aaaaacatgg
     1981 gaagcaagaa caggtttctt ttctatttac ttcactgatc ggtcagattt acaatgtcgt
     2041 ctacttgatt tttgtttgtt catgtttttg atgcgtatct tcacttttcg gaataaataa
     2101 attaagtaga tttcgctgtt ccttagaatg ttgacaagga aacaaaatta ctatacacta
     2161 tttttgtttc caagttaaca ttattgcata aaggagtttt tacatgtaca gtttcacaaa
     2221 taaacattct aaagtttttg gatacgtctc tgtcttattc actggattag gatttactat
     2281 cgtcagtcct gtacttccgt ttttaacgct gccttattct cactccatac atcagcaagc
     2341 tttctatatc actttgctca tgtctgttta tgctctagct gccttcttat ctgcccccat
     2401 tctcggtagc ttaagtgatc attttgggcg tcatccgatc cttattatca gtttattagg
     2461 atcaagtatc ggatatttgc tttttggttt aggaaattcg atatggatgt tgttccttgg
     2521 tcgtatcatt gaaggattga cggcaggaga aatctccacc ttatatgctt actttgccga
     2581 catcacggaa cctaatgaaa gaaccaaagt ttttggatgg atgggtgctt tagtcggtat
     2641 aggaacaacg ctgggtccta ttataggggg tctattagct gagttgggga attctgttcc
     2701 tatatttatt ggagcactct ttactttctt aaatgccgtt tatggctaca catttatgct
     2761 agaaagcctt ccaataaaaa aacgttcagt agatttaagt ttctcccatg taagaccttt
     2821 tcaccaatta caacaattat tcaagatttc atccgttatt cctttattga cagcaggttt
     2881 tgctgtttgg ttggcagctg gctctcttca atctatcttt tctcaatttt cgattgacgc
     2941 ctttcagtgg aaggctgggt tggttggttt aagcttctct ttgattggaa tcttggatac
     3001 tgtctcgcaa ttgttcatta tgcctcgatt gctcaaaaaa ttttcagaac agcagatcac
     3061 aagaataggg atgttcagtg aaatcctcgc ctatctattc atcacattat ctggaattct
     3121 tctactgccg attttgtttt tatttgggat tatctgttat ggtttcggtg attctatttt
     3181 tactcctgta ttcaatggcc aactatccaa ttctgtctca gataaccagc aaggtcttgt
     3241 aatgggcggt acccaaagta tacaatctct atcgcgcgtc atcggcccgc taatcgcagg
     3301 acagctttat gctgcagcac catttcttcc gacggtattc ggatttttgc ttatgatttt
     3361 tgcctattat cactacaata aaaaatagta gcttcatttg ctacttcgta taaattctct
     3421 gtatcgaccg ataaaacgaa atactgtgtc aaattcttct tttgacatat tttcaaatac
     3481 agtagcatct cgctgtaccc atttttgatg gagttgctcg tgtctattcc ttattttttg
     3541 tccgattggc gtcaattgaa aatagatttc ttttttattc tcttcgcttt gataagtgtt
     3601 tatgatccct ctagctatca gtttttttgt tagcttactg atggctcctc tagtcatgta
     3661 actagcattc gctaattttg tcacgttagg atcagggatt ttcccgatat gttcaatcac
     3721 ttctagttca tttagagtaa atcctgcaaa ttctttttcc atttctggtt tattacgatg
     3781 aataatctca ttagctaaat ccagatattc ttctattagc agttgtcttt tatccatttc
     3841 tacaccttct ttttctttca ttatattgtt tttttgtttc ctattcaaca aagataaaaa
     3901 tattttgtag gaaaacccaa tctgaaacca agaaaaaaag gggttgacaa agcttgtcat
     3961 aacccctttt tctgattaaa agatagtaaa gagacagtca tcggtaattg tgatgatttt
     4021 ttgtcatcac ttaaggatga gcggcctttt caaaaactgt ctttaaagat catcatcagt
     4081 ttctagcaca tatggctgac cttcatatgt gtactctgta ttcaatgtgc cgtcattttc
     4141 atctgagtag atgcgtctac tgaaaggaaa acgatgagaa acattatctt tggcgtcatt
     4201 tgctggcatc gtgtaatatt cctcttgcgt ttcattcaac tgagagagta cttgatctgc
     4261 caactcatca atattgatcg ttaatttctt cgaagcactt ttataatctt ctaaaaatgc
     4321 atcttgttgg acaaaatatt ctttattcat cttctttctc acctttcttt tcaaaatcaa
     4381 ataaagctgc ttgttctttt atgtaacagc tttatctttg ctgcttattg tgacaaaaaa
     4441 ttcgaagaaa gacaactaat tgtacttttc acaaaaaaag acagagggtc gtgacaaaat
     4501 tgtgtcacaa ccctttttct ttattgtgca gtttcgattt cgcctagcgc ttcttgtgca
     4561 agtaggttca agtaattcca tgggcggtca aaatgtggtt ggaagaagaa atctgaaagt
     4621 gcgagatctt ctacagtcat tttattttga acagctaaag acatggtatt tgctgattgt
     4681 gtaatgtcgt atttggacat gaactgagca cctacgatac gattcgttcc tttttcatag
     4741 actagctcca tcattacttt ttcagtagtt ggcatgaact ctggacgata attatcttca
     4801 aatacagtcg cttggacttc tagatcgttc aaagttgcgc tttctttggt cacacctgtc
     4861 gaaccgatcg tccagccaaa taggtaaagt cctgaagttc cttgagtacc acgatacgcc
     4921 attttatgat ctgtcaagtt atttcccaca agtaaacctt gacgaacggc atttgttgcc
     4981 aaaggaatat agttttgcgt gccacttgga ttgtagttga ctactgcaga gtctccagct
     5041 gcaaaaatgt ctggatcact tgtctgcatg tattcattca ctttgattgc cccgtttggc
     5101 agtgtatcta cttttccttc gatcagtttt gtatttggac ggaagcccac acataaaatg
     5161 accatatctg tatcaaatgt atctgttggt gtagctactt gtttcacttt tccttcttca
     5221 tctgtgataa attcagtaac attttcgcct agcgcaagag taacaccgcg gtctgtcaat
     5281 tctttttcta agatatctgt gaatggttta tctagatatt tattcaagat gcgatctaat
     5341 ccatcaatca atgtcacttg ttttccagat tcgacaaaag cttctactaa ttcaataccg
     5401 atatatccac caccaacaat tactacacgt tttgcttctt tcgcttgtgc aataatttca
     5461 tttgcttgat tgtagttttt gcataggagt acatttttcg catcgattcc actaatcggt
     5521 gggataatag gccatgaacc agttgtcatc actaatttat cataagaaac cgtttctgtt
     5581 tctcctgttt tcagatcttt tgccgttact gttttatttt ctgtatcgac gtctgttaca
     5641 tcgtgttcca ttttaactgt tgctcctaat gaagataatt cttcaggatt tgagtagaac
     5701 aagcctgctg ggtcctttac tactccgccg acataaagtg cgataccgca agataagaaa
     5761 gaaatattgt catttctttc atatactgtc acttctgctt ctggatgatt ttttaaaata
     5821 ctttttacag ctgcagtacc tgcatgagta cagccaatta ctactacttt catttatggt
     5881 ccctccgatt aacattttgt ccttcgacaa aactactata acagaaataa aacggataca
     5941 acagtttaat gcttgtgaat tcacaaaggt ttgtttgtag tttcattcct tttcaagcat
     6001 aagaacccaa tatctacaag attcttattg tgataatatt cactttgata tctaagattg
     6061 tgaataaaag acaaaaaaat aaaaaagcta gcgttttttt acacagctag ctttttttag
     6121 gtataacact tatagaaact gtttttacat ttgcgccttt tttgctgact ctttacaatc
     6181 cggacagagt ccatacaatt ccaaccgatg ttcattaatc tcaaaatcag tcagtctgct
     6241 cgcagccatt tctatatctt caagccctgg gtaatgaaaa tcaacaatct taccgcagtt
     6301 ttcacaaatt acatgataat gacgctttga actgaaatca aatcggctag aagcatcgcc
     6361 gtacttcatt tcttgaacaa atccaatcga agtaaataag cgaaggttat tgtaaacagt
     6421 cgcaacactc atatttggaa accgatgttc aagtgcttta taaatctcat cagccgttgg
     6481 atgggaatga tgttcaatca aatattctaa aaccgcgtac cgttgaggtg tgattcgaat
     6541 gttctctttt tttaactttt cgataacctc ttcgactaac ttattttcca tgtaaacacc
     6601 ccacgttgtt attctcgtct tatcatacta tttttacatt ttaaaatcca cttaacatga
     6661 aaagaaaatt caattttatt gtattttaat gaaaaaagac gtaaaacagt cgcgttcact
     6721 attttacgtc tctattcaaa taatttttct catcataaat ttttgtgtgt tcttttcatc
     6781 tttaaaagtt ttttacataa acctatctgg agaaaaacca agtgccttat atctcttggt
     6841 aaagggctgt agataaataa cgttcaccat tgtcgggaac cacagctaat accttcttgc
     6901 cttttcctaa ttcttttgcg acttttagcg cggcagcaat tgcagcgcca gatgagatgc
     6961 ctactaaaat cccttctttt cgcccaactt ctctagctgt ttccatcgct tcgtctccac
     7021 taatagaaag gactttatca tacacagaag tatccagtgt tttaggaaca aaacctgtac
     7081 cgattccttg gattttgtga ggtcccgctt ctttcccttc taaaatagca gattctgctg
     7141 gttctacccc gattaattcg attttcggat acacacgttt taattctcgg ccagcgccag
     7201 tgatcgttcc tcctgtaccg atacctgcaa caaacgcgtc caagccattt actccaaatg
     7261 cttgatgtat ctcaggacca gtcgtcttct catgcacaag aggatttgct tcattttcaa
     7321 attgtaatgg taaaaagtag ccgttttctt ttgccaaacg ttctgcttct ctgattgatc
     7381 cagaaatacc atccgcacca ggagtcaaaa tcaactctgc gccgtaccct ttcatcaata
     7441 aacgtctttc gatactcatc gtctcaggca tcacgatgat tactttatat ccttttgcaa
     7501 ctcctaccat cgacaatccg attcctgtat ttccagaagt aggttcaatg atagtatcac
     7561 ctggtttcag tagaccatcg tgttctgctt tttctatcat acttaaagca atacgatctt
     7621 ttacacttcc tcctggatta aaaaactcaa gctttacaaa aacatctgct gaatcttctg
     7681 gtacgatttt atttaatttg acgattggcg ttttgccaat caattcggtg actgagttat
     7741 agatagctgt catcttcaat tcctccttgt ctttgctctt cttacttatc ataatacaat
     7801 ctgttcattt atggcgagtg attcgtcttt gactagcaaa aaaaagacaa agatatacca
     7861 tatatggtag tttctttgcc tttttatcta aatcaaacgt ttagttcttg accaactaat
     7921 aaagaataac tggacataga catgccattt ttcgccatta gatcatcaac agacatgcca
     7981 aatttttgtg caatatccca tagcgtatca cctgacacga ctgtatatgt ctgaccatta
     8041 tttgtattta catctgttgt tgaagatgat gacgttgaag tcgtcgtagt agtcgttgtt
     8101 gttgtacttg tggtagccga acctgttgat ggcgtatcaa aacgggttaa attataagta
     8161 ttaatcaatg agttcaattt ggccccataa tttggatcgg tagcataacg acctgtcaag
     8221 tacgctgttg catcataaaa acttgttgta ttgcttttcc acacgccagc ataatgataa
     8281 tcgcccgtgg aaaaactggt tgaacgaaga acattcgcat ggtcttggaa ggattcccaa
     8341 taagaagaat attttcggaa tggttcattc atcgttaccc attgtccgtt acatattctt
     8401 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
     8461 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
     8521 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
     8581 cgataaacgc ttgaggatca gcaactgttt gagatccttg ttcatctgca tgaacatgtt
     8641 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttcc
//
LOCUS       DAXLPI010000104         8603 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXLPI010000104 DAXLPI010000000
VERSION     DAXLPI010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.105"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACF3T5_002171"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="ACF3T5_002171"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFD3632734.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="ACF3T5_002172"
     CDS             complement(1098..2201)
                     /locus_tag="ACF3T5_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFD3632735.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACF3T5_002173"
     CDS             complement(2291..3226)
                     /gene="pplA"
                     /locus_tag="ACF3T5_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFD3632736.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="ACF3T5_002174"
     CDS             3528..5462
                     /locus_tag="ACF3T5_002174"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFD3632737.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="ACF3T5_002175"
     CDS             5919..6335
                     /locus_tag="ACF3T5_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFD3632738.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="ACF3T5_002176"
     CDS             6349..6915
                     /locus_tag="ACF3T5_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFD3632739.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="ACF3T5_002177"
     CDS             6938..7924
                     /locus_tag="ACF3T5_002177"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFD3632740.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="ACF3T5_002178"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="ACF3T5_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFD3632741.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1657 c         1751 g         2668 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaagcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttaggtcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DAXLPI010000105         8501 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXLPI010000105 DAXLPI010000000
VERSION     DAXLPI010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.106"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..353)
                     /locus_tag="ACF3T5_002179"
     CDS             complement(<1..353)
                     /locus_tag="ACF3T5_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632742.1"
                     /translation="MIDKQITNILQSYKKQQIFKIEDFLLSEIDEDNLQETIDFVVSD
                     DVSKKINFSDELYDGNEYEGVFLEGNQYLLSSSEGKVMIIDMLSEAHGVNIKDTRVQF
                     DEEKFIKLITNKKEIL"
     gene            complement(370..1437)
                     /locus_tag="ACF3T5_002180"
     CDS             complement(370..1437)
                     /locus_tag="ACF3T5_002180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFD3632743.1"
                     /translation="MAKPSTPATYKVKAGDSVWGICEKYGLSISKFVQWNKIKNNTIH
                     PGQKLTVKQPVSSGKPTTKVPASTNPVTHKVSYGESLWLISSKYGVTVDELRKQNGIK
                     GDLIHPGQVLVVKKGMTTSHSTPVGKSGTSYTVKAGDSVWLIANRYGVSMDDLVKWNK
                     IKNYTIHPGQVLTIQTIVSTPAKPAITTTPKVKAKPAITTTPKAESKSGVTTTPKVDE
                     KPVITTIPKVKEQPVVTTVPKTESEVLVEKASGDGSRLDINIKNFEIKNKHLNSSTAK
                     RARKFNVSSAEEANKIVQDALKNGKLVEAIPNGVGSQGQNSFSSIIDTGKVIGTKGET
                     HIKIVYDELKNVWTTYPVPKP"
     gene            complement(1959..2471)
                     /locus_tag="ACF3T5_002181"
     CDS             complement(1959..2471)
                     /locus_tag="ACF3T5_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632744.1"
                     /translation="MKIKSYEDKLVKDDIEVDYYVPITVSFPENDDKYNLGEFYYYRF
                     VNEYESFVEIKINSVTQKIVEVVFTSINDIEDSNLFIGEYAEKNPVIQTDIFDENSII
                     TKESGFKISREHKKLCFKLDKEKMDSVVKMSNHVSLFLNEDSEIIGLEFSSFSDNEWA
                     ELNESIKSTT"
     gene            complement(2490..5453)
                     /locus_tag="ACF3T5_002182"
     CDS             complement(2490..5453)
                     /locus_tag="ACF3T5_002182"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFD3632745.1"
                     /translation="MLKKVLRFGVCISIWLVTSSTFVPSMIAVADSVAIQETIASDSE
                     GEEKLTERSDDASDGQTEKVEVQKTETTQTTVSSSSDESSDLTQSSEETAPEEVDEST
                     EESSDPQEQTISHEEETKEAVDSSTIDFEGTRSSESFGKHTSPSIDKPNEKIAEPVSS
                     NEGAVVSRRSRRDLSAVSSNHPEEEPVVAFDEKAFDAAIDQYIDHATEEELGLVINSS
                     NEKELADALEYLLLNQLDESFGVEELDELKKFLTADEIERLENESSLETFEQLLTQYY
                     NDREQKLEEGAAFSDFFLASTDEEIDRMLEVKDEQALEQVISEISASRNSQTSGKRNK
                     RFLVIPLLVKGAMLVIGAGVAAYSSHQFYKTMEAQRAEKKRMVERWRYHEQQAQKRHQ
                     AAQQARAKAVARAKLQAAARAKAQATQRARAQAAAKARKQAQNWAKQQAAARARAQAA
                     ASARRKSNASYVPPVTRPKPVIKAQPKVPAKPVAKPVTKPTSPAKPKPTTTPKNTMHQ
                     VRAGESVWSISHKYGISMNDFIKWNKIKNNLIHPGQQVIVKQPASTSKPVSKPATKSS
                     TPATYKVKAGDSVWGICEKYGLSVSKFVQWNKIKNNTIHPGQVMHLQEPKPASKPAPK
                     TSPTTNTTYTVKAGDSVWLIANMHGISMNDLVKWNKIKNNTIHPGQKLTVKQPVSSGK
                     TTTKVPASTNPVTHKVSYGEPLWLISSKYGVTVDELRKQNGIKGDLIHPGQVLVVKKG
                     MTTSHSTPAGKSGTSYTVKAGDSVWLIANRYDVSMDDLVKWNKIKNYTIHPGQVLTIQ
                     TIVSTPAKPAITTTPKVESKPAITTTPKVESKPGVTTTPKVDEKPVITTLPKVKEQPV
                     VTTVPKTESEVLVEKASGSTGTVWDDIKGTQPPREGTTIPKSFEIIVNGEKLWVNPNG
                     TKHMVEYSTRNLSHGQKLTEQQLLRSFQGAVEQATSTGIKFETKIIIDNWELIFSPAR
                     EAGQLPVIKHAVYLP"
     gene            complement(5699..6166)
                     /locus_tag="ACF3T5_002183"
     CDS             complement(5699..6166)
                     /locus_tag="ACF3T5_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632746.1"
                     /translation="MKKSLIVLIVGLSIFGSVLFIGNSLFSNKDVTEQSPASTTEEVS
                     PELLLETDVLDTKKPETIPSENELYQGDLSFEYADPEKPYVLTIRFIDKANHEYVGPD
                     KKPLKREKELILGKAKGVVHVVVKANRSMLTHIQKLKDIQVSMTLNERNEKTE"
     gene            complement(6188..6340)
                     /locus_tag="ACF3T5_002184"
     CDS             complement(6188..6340)
                     /locus_tag="ACF3T5_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632747.1"
                     /translation="MKKKGIPYSPKPRNAFERGLFKYFEGERDQEEKEKNQKKKSSQE
                     KENIEK"
     gene            6841..7182
                     /locus_tag="ACF3T5_002185"
     CDS             6841..7182
                     /locus_tag="ACF3T5_002185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632748.1"
                     /translation="MKANTPYKVGDIFYSSWGYEQTNVTFWQIIKLTKKTAWFRPIKK
                     QTVKNYTSMSGETMPIPNEFIDYPLARTKDSIVRKRINPALYPNELYGNRSWDTFYRY
                     DNQPVYESSYY"
     gene            7375..7668
                     /locus_tag="ACF3T5_002186"
     CDS             7375..7668
                     /locus_tag="ACF3T5_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632749.1"
                     /translation="MLRTNAIPIVDSYDRNTNNYLGSFEQTDENILNYVAGLSPFESV
                     RLVEHTTDTLILTTIGYFFDQVSDQQWLQQILPKLIAKQTGKSTIEAVKIFFY"
     gene            7724..8272
                     /locus_tag="ACF3T5_002187"
     CDS             7724..8272
                     /locus_tag="ACF3T5_002187"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632750.1"
                     /translation="MSILQPFGEPIERTEFIQHYMKLFVQVIKHTHQIDEFYSKEIEY
                     LLAEKQKIALLYDYFVEMYDRAPDYFYLSDTLTTNFLAKEHLFASHTKNLMCVEHFVN
                     TYLHLLKIQKICTFEAFQTDYLFILDREAYHAKLAFEKQNQAIEGYPELRIQNNSFLQ
                     QRLLKQLINGFHQRNKAYQKDQ"
BASE COUNT         2422 a         1670 c         1400 g         3009 t
ORIGIN      
        1 agaatttctt ttttgttcgt gattagtttg ataaattttt cttcatcaaa ttgaactcga
       61 gtatctttta tattaacacc atgagcctca cttaacatat caattatcat tacttttcct
      121 tctgagctag atagtaaata ttgattacct tctaaaaata caccttcgta ctcgttccca
      181 tcatataatt catcactaaa gttaattttc ttactaacat catcagaaac tacaaaatct
      241 attgtttctt gtaaattatc ttcatcaatt tctgaaagta aaaagtcctc tattttaaat
      301 atttgttgtt ttttatacga ttgcaaaata ttcgttattt gtttatcaat cactttgctt
      361 acctcctatt taaggttttg ggacaggata ggtagtccac acatttttta attcatcata
      421 aactattttt atatgtgttt cccctttggt tccaataact ttccctgtat caataatact
      481 agaaaaagaa ttttgtcctt ggctccctac tccatttggt atagcttcta caagttttcc
      541 attttttaat gcgtcttgaa ctattttatt agcttcttcg gctgaagaaa cattaaactt
      601 tctagcccgt ttagcagttg aactattcaa atgtttattc ttgatttcaa aatttttaat
      661 attaatatcc aatctacttc catccccact ggctttctca accaatactt ctgattcagt
      721 cttaggaacg gttgtgacca caggttgttc ttttactttc ggtattgttg taattactgg
      781 tttctcgtcc acttttggtg tggtagtcac tccagatttt gattccgctt ttggtgtcgt
      841 ggtaatcgcc ggttttgctt ttacttttgg tgttgtcgtg atcgcaggtt ttgctggtgt
      901 agaaacaatt gtttgaatcg ttaagacttg acccggatgg atcgtataat ttttgatctt
      961 gttccatttc actagatcat ccatgctcac gccgtatcga ttagcgatca accaaacaga
     1021 atcccctgct tttacggtat agctggttcc gctttttcct acaggagttg aatgactcgt
     1081 ggtcatacct tttttgacga ctaatacttg gccggggtga atcaaatcac ctttgatgcc
     1141 attctgtttg cgtaattcat cgacggtcac accatattta ctactaatca gccataaaga
     1201 ttcaccataa gagactttgt gggtgactgg atttgtagaa gcgggtactt ttgtcgttgg
     1261 tttccctgat gaaacaggct gtttgactgt caacttttgg ccaggatgga tcgtattatt
     1321 cttgatctta ttccattgga caaattttga aatggataac ccatacttct cacaaatccc
     1381 ccaaactgag tctcctgctt ttactttgta cgtagctggc gtacttggtt ttgccactgg
     1441 tttggacgtt ggttttatag tagattatct tctggaaatt ctcaaattaa ccttttacat
     1501 gcctctaaag tggatataaa attttcaact gaagaagcaa aggtcactaa tagtaaatat
     1561 gaacactatt tttgaatctg gaaaagagaa aattaatatg acaaatgact aaatagaagt
     1621 tgtgaaggta cttgttataa aacaatttct aaaaggagcg gaaggcaatg agaaaaatga
     1681 ttcttctgat ccgtaaaaca ctataaagaa agtcaataga tcattagtct ctcctaatat
     1741 tgctattgac tttctttatt gggcatgtgc ttttgatagt gcccgaggaa ctcacaaaat
     1801 acctagtacc ttggattatc cctcgaacct tttcagcata acatgcccac cgtttatttt
     1861 agcatttgaa tttgttcttt caaaattctt ttgtttcatc ctcagaatct agcaaaatag
     1921 ctgttgaaaa tcctatcttc ttctcattta aaattaaatc aagtggtaga ttttatactt
     1981 tcattgagtt ctgcccattc attatctgaa aagctagaaa actctaaacc aataatctca
     2041 ctgtcttcat ttagaaataa agatacatga ttagacatct ttacaacaga atccatcttt
     2101 tctttatcta atttaaaaca taattttttg tgttctctag atattttaaa cccagattct
     2161 ttagttataa tcgaattttc atcaaaaata tctgtttgga tcactggatt tttttctgca
     2221 tactctccaa taaataaatt ggaatcttca atatcattga tggatgtgaa tactacttct
     2281 acaattttct gtgtaacaga attgatttta atctcaacaa atgattcata ttcattaacg
     2341 aatctataat aataaaattc tcctaggtta tacttatcat cattttctgg aaatgatact
     2401 gtgattggga cataatagtc aacttctata tcatctttga ctaatttatc ctcataactt
     2461 ttaattttca taatttgctc ctttcatagt tatggtagat atacagcatg tttaataact
     2521 ggtaattgtc ctgcttctct agctggacta aatattaatt cccaattatc tattattatt
     2581 ttagtttcaa attttatccc tgtagaagta gcttgttcta cagcaccttg aaaactcctt
     2641 aacaattgtt gttcggtaag tttttgtcca tgagatagat ttcgagttga atattctacc
     2701 atgtgcttgg ttccatttgg atttacccac aacttttccc cattaacaat tatttcaaac
     2761 gatttaggaa tagtagttcc ttctcttgga ggttgtgtac ctttaatatc atcccagact
     2821 gtacctgtag acccactggc tttctcaact aatacttctg attcagtctt aggaacggtt
     2881 gtgaccacag gttgttcttt tactttcggt aatgttgtaa ttactggttt ctcgtccact
     2941 tttggtgtgg tagtcactcc aggtttggat tccacttttg gtgtcgtggt aatcgctggt
     3001 ttggattcta cttttggtgt tgtcgtaatc gcaggttttg ctggtgtaga aacaattgtt
     3061 tggatcgtta agacttgacc cggatggatc gtataatttt tgatcttgtt ccatttcact
     3121 agatcatcca tgctcacgtc gtatcgattg gcgatcaacc aaacagaatc ccctgctttt
     3181 acggtatagc tggttccgct ttttcctgca ggagttgaat gactcgtggt catacctttt
     3241 ttgacgacta atacttggcc ggggtgaatc aaatcacctt tgatgccatt ctgtttgcgt
     3301 aattcatcga cggtcacacc atatttacta ctaatcagcc ataaaggttc accataagag
     3361 actttgtggg tgactggatt tgtagaggcg ggtacttttg tcgttgtttt ccctgatgaa
     3421 acaggctgtt tgactgtcaa cttttggccc ggatggatcg tattattctt gatcttattc
     3481 cacttcacca gatcattcat actgatccca tgcatattag cgatcaacca gaccgaatct
     3541 cctgctttta ccgtatacgt cgtattagtc gtaggagaag tttttggtgc tggttttgat
     3601 gccggtttcg gttcttgtaa atgcatcact tgtcctggat gaatggtatt atttttgatc
     3661 ttattccatt ggacaaattt tgaaacggat aacccatact tctcacagat cccccaaacc
     3721 gaatcccctg cttttacttt gtaagtggct ggcgtacttg attttgtcgc aggtttggac
     3781 actggctttg atgtacttgc tggttgtttg acgatgactt gctggccagg atggatcaga
     3841 ttatttttga ttttgttcca tttgatgaag tcattcatac tgatgccata cttatgcgaa
     3901 atagaccaaa cagattctcc tgcacgtact tgatgcatcg tatttttggg ggtcgtcgtt
     3961 ggcttcggtt tcgctggaga agttggtttc gttactggtt tggctactgg ttttgcaggt
     4021 actttgggtt gggctttgat tactggtttc ggacgagtta ccggaggaac ataggacgca
     4081 tttgactttc ttcgtgcact tgcggccgcc tgtgcacgag ctcgagcggc ggcttgttgt
     4141 ttagcccaat tttgtgcttg ttttctcgct tttgctgcgg cttgggcacg agctcgttgt
     4201 gtggcttgcg cttttgcacg ggctgctgct tgtaatttgg cacgggcaac ggctttggcg
     4261 cgggcttgtt gtgcggcttg atgccgcttt tgtgcttgtt gttcatggta acgccagcgt
     4321 tcgaccattc gtttcttttc cgcacgttgt gcttccatcg tcttatagaa ctgatggctt
     4381 gaataagcag ctacccctgc cccgattacc aacatcgctc ctttgacaag caatggaatg
     4441 actaagaagc gcttgtttcg ctttccactt gtttgactat tccgagaagc actgatttca
     4501 gaaatcactt gttccaatgc ttgttcatct tttacctcta acatccgatc gatttcctca
     4561 tcggtacttg ctagaaagaa gtcactaaat gctgctcctt cttccaattt ctgttctcga
     4621 tcattatagt attgcgtaag cagttgttca aatgtctcta acgacgattc attttccaat
     4681 cgttcaatct catctgccgt gaggaacttc tttagttcat cgagttcttc tacaccaaag
     4741 gactcatcca attgattcaa caagagatat tctaaagcat cggccaattc tttttcattg
     4801 cttgaattta tgactaaccc taattcttct tctgttgcat gatcaatgta ttgatcgatt
     4861 gcggcatcaa aggctttttc atcaaaagcg accaccggtt cctcttctgg gtgatttgaa
     4921 gaaaccgcag aaagatctcg gcggcttcgc cgtgaaacaa ctgctccttc atttgaagag
     4981 actggttcgg ctattttttc gttaggttta tcaatacttg gagaggtatg tttaccgaat
     5041 gattcagaag atcgtgttcc ttcaaaatca atcgttgatg aatcaactgc ttccttcgtt
     5101 tcttcttcat gagaaatcgt ttgttcctga ggatccgaag actcttcggt agactcatcg
     5161 acttcttctg gcgcagtttc ttcgctggac tgggtaagat cacttgattc atctgaactg
     5221 ctcgataccg tcgtttgagt agtttctgtt ttctgtactt caaccttttc agtttgtcca
     5281 tctgacgcat cgtccgagcg ttctgtaagc ttctcttccc cctcgctatc agatgcgatc
     5341 gtttcttgta tcgccaccga atcggccact gcaatcatac ttggcacaaa agtggaagat
     5401 gttaccaacc atatacttat acatacccca aatcgtaaca cttttttcaa catatttttc
     5461 ttcctttctt catttacacc taattaaaat aacgatcatt atcatactga aaattgtaga
     5521 aacaaattta aataggtgtt taaagaaata atatcatcat ttttaatgat gataaataac
     5581 agggaagaaa aaaatgacct ttcatttttg taatatgatc aagcaactat aaagatggaa
     5641 caaaataaat aaaccgataa aagtagaaca gatattccca aaacatgtct gatttccgtc
     5701 attccgtttt ctcatttctc tcatttaatg tcatggacac ttggatatct tttaacttct
     5761 gaatatgggt taacatcgat ctattggctt ttacaactac gtgtacgaca cctttagctt
     5821 ttcctagaat caattctttc tctcttttca atggcttttt atcaggccct acatattcat
     5881 gattcgcttt atctataaac cgaatcgtta gtacatacgg cttttccgga tccgcatatt
     5941 caaatgaaag atctccttga tagagctcgt tttccgaagg aattgtctca ggtttctttg
     6001 tatcaagaac atccgtctct aataatagct ctggagatac ctcttccgtt gtgctagcag
     6061 gggattgttc cgtaacatct ttgttagaaa acaacgagtt tccaataaat aaaacacttc
     6121 caaatatact taatccaaca atcaatacta ttaaactttt tttcatactt ctctattttt
     6181 tcacctacta tttttctata ttttcttttt cttgactaga cttctttttt tgattcttct
     6241 ctttttcttc ctgatctctt tcgccttcaa aatatttaaa taaccccctt tcaaaagcat
     6301 ttctaggttt cggagaataa ggaatgcctt ttttcttcat acaaacacct cttttaacta
     6361 tttgatctat tttataccac cacattatca aaataacaaa tgtattaaaa ggaatttctt
     6421 attaaaacca tcttgacctt ttgggagatt tcagtgcgat ttctttttat tcccatactt
     6481 ttttcgttgg tcgctttcgt atacccaatc taagcgatca tcggcagtag tgcttttaca
     6541 acgaatggta ggacaaaaca aggggcggaa aaactttttt gatcatttca taaaaaactt
     6601 tttcctacag cattcccttg ttttctctta ccacacgttg tgacctaaaa gcacgccgac
     6661 gaacgcttag ctatacgaaa atgaccaacc aaaacaaaaa agtgaggtga atggaaacaa
     6721 aaagaaattg aaatctccca aaaggtcacc cgcctcggag acaacttgct gccgaaaatg
     6781 gttggttgat tcggctaagt atcaaataag gcaaatcaaa tattattgga ggaatagatc
     6841 atgaaagcaa atacaccata caaagtagga gacatttttt atagtagttg gggctatgaa
     6901 caaaccaacg ttactttttg gcaaattatt aaattaacca aaaaaacagc atggtttcga
     6961 cccatcaaaa agcaaactgt aaaaaactat acttctatga gtggagaaac tatgccaata
     7021 ccaaatgaat ttatcgatta tccgcttgca cgaaccaaag acagcattgt tagaaaacga
     7081 attaatccag ccttatatcc caatgaatta tacggtaatc gcagttggga tacattttac
     7141 cgctatgata atcaaccggt ttatgaaagc agttattact aagagggtat ttccctctta
     7201 gtcaacgtta caaaaataaa aggagcaaga acaatgaaac caataactct atatgaaatc
     7261 ttaggtgaag aacggtctga caatttacca acaacgcata aattaagagt tcttgcttct
     7321 aaatctatcg gtaaaaaatg ataactgagt atttctaatc aaggagagat tgcaatgcta
     7381 agaacaaatg ctattcctat cgttgattct tacgatcgga ataccaataa ctatttagga
     7441 tcttttgaac aaacagatga aaatattttg aactatgttg ctgggttaag tccttttgaa
     7501 tctgtacgtt tagttgaaca tacaacagat acactcattt taacaacaat cggctacttc
     7561 ttcgatcagg tatctgacca acaatggtta caacagattt tacctaaact tatcgcaaaa
     7621 caaactggaa aaagcaccat cgaagcagtc aaaatatttt tttactaaat acatgtattc
     7681 attctcaagg gattttttcc cttgagtgaa aggagaaaga atgatgtcca tacttcaacc
     7741 ctttggtgaa ccaatcgaac gaacagaatt cattcaacac tatatgaaat tatttgttca
     7801 agttattaaa cacactcatc aaattgatga attttattcc aaagaaatag agtatcttct
     7861 agcagaaaag caaaaaattg ctttacttta tgactatttt gtcgaaatgt atgatcgcgc
     7921 accagattac ttttatttat ctgatacact tactactaac tttttagcta aagaacatct
     7981 ttttgcctca cacacaaaaa accttatgtg tgtagaacac tttgtcaata cctatcttca
     8041 tctgttaaaa attcaaaaaa tctgtacatt tgaagcattt caaacagatt atctatttat
     8101 tttagatcgt gaagcatatc atgcaaaact agcatttgaa aagcaaaatc aagccattga
     8161 aggctatcca gaattacgca tccaaaacaa ttctttttta caacaacgat tgttgaaaca
     8221 gctaataaat ggattccatc aacgaaataa agcctatcaa aaagatcaat aactggttct
     8281 ttttgatagg ttttttccat caaaaacaga aacttctttt tttaattgtt tgattacttt
     8341 tttagtcata aagatcattc aaattttttt aaataaatta tccatatctt ctaatattaa
     8401 tggaacatct gcaattattc gatccctatt cgtaccaaat aattcaatcg atgaagcatc
     8461 aaagaaagaa actatttttt ctaaattact aaatgtagtt a
//
LOCUS       DAXLPI010000106         8452 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXLPI010000106 DAXLPI010000000
VERSION     DAXLPI010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.107"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            78..2687
                     /gene="clpB"
                     /locus_tag="ACF3T5_002188"
     CDS             78..2687
                     /gene="clpB"
                     /locus_tag="ACF3T5_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFD3632751.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2838..3524)
                     /locus_tag="ACF3T5_002189"
     CDS             complement(2838..3524)
                     /locus_tag="ACF3T5_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632752.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3502..3996)
                     /locus_tag="ACF3T5_002190"
     CDS             complement(3502..3996)
                     /locus_tag="ACF3T5_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFD3632753.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4016..5236)
                     /locus_tag="ACF3T5_002191"
     CDS             complement(4016..5236)
                     /locus_tag="ACF3T5_002191"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3632754.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5383..7218
                     /gene="lepA"
                     /locus_tag="ACF3T5_002192"
     CDS             5383..7218
                     /gene="lepA"
                     /locus_tag="ACF3T5_002192"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFD3632755.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(7312..8439)
                     /locus_tag="ACF3T5_002193"
     CDS             complement(7312..8439)
                     /locus_tag="ACF3T5_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3632756.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         2715 a         1620 c         1700 g         2417 t
ORIGIN      
        1 gaccaaagcg atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat
       61 taggagggta tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg
      121 ctgaagctca acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat
      181 ggaaaatctt tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg
      241 atgtcgaatc ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg
      301 gtggaaacgt ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag
      361 cagaccaaat acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag
      421 cgttgatgaa attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg
      481 aaaaagaatt gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa
      541 accaagaaga acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca
      601 aaagcggaaa aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta
      661 ttctttcacg aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa
      721 cggcaatcgt tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa
      781 aagataaaac gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg
      841 gtgaatttga agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta
      901 tcctcttgtt catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta
      961 tggatgccgg aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg
     1021 ccacaacttt agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct
     1081 ttcaaaaggt attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat
     1141 taaaagaacg ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag
     1201 cagctacttt atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt
     1261 tgatcgatga agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag
     1321 atcaagttac tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat
     1381 cagatgatgc aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg
     1441 aagaagcaaa tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt
     1501 cagcaaaacg tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact
     1561 atgatttaga acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat
     1621 tgaaggaatt agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta
     1681 ccgaaaatga gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg
     1741 tagaaggtga acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg
     1801 gtcaagatga agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc
     1861 aagacccaaa tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa
     1921 ccgaattagc gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga
     1981 tcgatatgag tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag
     2041 gctatgtagg ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata
     2101 caatcgtctt gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac
     2161 aagtcctaga tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata
     2221 cggttctgat catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg
     2281 atggaacgat tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca
     2341 aaccagaatt cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca
     2401 acgtgaaagg aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag
     2461 aaatcctgct gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac
     2521 cagcatatgg tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag
     2581 ctaaagaaat cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg
     2641 atggacaact tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga
     2701 ctataaaaga aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac
     2761 agtgttccag aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc
     2821 tgtcacaacc tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa
     2881 cgctcatctt ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta
     2941 tttgtcagca agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca
     3001 tcagaagcag caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag
     3061 aactcccaaa acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa
     3121 ggactggttt ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga
     3181 gctgtcagga ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg
     3241 atcaaatgca tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa
     3301 acgattgctg ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc
     3361 aatgcaatcc ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt
     3421 tgtgtgataa attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct
     3481 atttgcacct ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac
     3541 ttctgaaagc aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat
     3601 tgtatataag ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt
     3661 tttcgggtgg gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc
     3721 tttcgcataa gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag
     3781 taaagcaatc attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg
     3841 actgtctgca taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga
     3901 aagctcagaa gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact
     3961 tttagcaaac attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa
     4021 aataatcgct aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt
     4081 cttcatctgt ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca
     4141 tcaagcaagt caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga
     4201 cacggacttg tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga
     4261 caggatcttc aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa
     4321 ggaattcttt tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct
     4381 gactatcttc aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt
     4441 agatcatgga aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa
     4501 gatagaacat ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc
     4561 ttggttcatg gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac
     4621 gccaatactc ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa
     4681 atttgtcata aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac
     4741 ttactgggat ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca
     4801 tgatttccca tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc
     4861 attgcttatt cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat
     4921 ggttagcata ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac
     4981 cgtgaatatt gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga
     5041 accatccgaa cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt
     5101 tattctcaaa cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca
     5161 attcattgaa ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa
     5221 aatctttttg atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt
     5281 ctacaaaaaa aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt
     5341 ggtataatgg aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg
     5401 aaagaacgcc aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa
     5461 tctacattgg ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa
     5521 gatcagctgt tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat
     5581 gctgtagaac tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac
     5641 accccagggc acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt
     5701 gccgttttag tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat
     5761 ttagcgttag acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca
     5821 gcagatcccg aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa
     5881 gctgtattag ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg
     5941 gaatatgttc ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac
     6001 tctatttatg atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc
     6061 aaacccggag acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc
     6121 ggggtcttct cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat
     6181 atcacagcaa gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca
     6241 gacaatcctg ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt
     6301 ggtctttatc caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg
     6361 caattgaatg atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg
     6421 ttccgctgtg gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg
     6481 gaattcaatc tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg
     6541 gatggtacga ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag
     6601 gatgtagaag aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca
     6661 gtaatggaac tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat
     6721 taccgagtaa atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac
     6781 aaattgaaat caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa
     6841 aaaagcaagt tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc
     6901 tttatcgttc accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa
     6961 aaactaatcc cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc
     7021 gttgcccgtt cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga
     7081 gatgtttctc gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa
     7141 caaatcggct ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag
     7201 gacgaaccga agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt
     7261 acatggattt gccaccggtt tgccaccggt ggtttttttc ggtggcaaaa atcaagaatt
     7321 ctttctaaga ttttccatga tatcgaccgt ttcatttctc atcttatctg ttacatgcga
     7381 gtaagtatcc atcgtaattg aaattctgct gtggcccaat cgttcagaga tttctttcat
     7441 ttttgcgcca ttttcgagaa gaagtgtagc atgagtatgt ctgagagaat ggaagttaaa
     7501 agagagggag agtgcattcg atatcctcct agtattccat ttcactacac ttggcgtaac
     7561 tagctcaccg tcttccttcg tacatactgc atttgaatca atgtagagct ttccatactt
     7621 cattcgattt tctaattgtt gtttcttatg ttttttcaga attgctagca aggtttgtcc
     7681 aataaaaatc gttcgattgg aactgcttgt ctttggcgta ccatataccc atgcgccgtc
     7741 attctttacc atttgtttct ctacagtaat tgttccattt gaaaaatcga catcatccca
     7801 cgtcagacca caaacttcgc caacgcgcat tcccgtataa aatccaatat tcaaaggaat
     7861 atagaaagga tgtccttcag gagtaatttc tagcatatga tcaaagtcct caagagaaat
     7921 gatttttaga tcttttttag tcgttggtcg tgcttcatat tttggtattt ttacatacag
     7981 cataggattt tgcttgatta atccccaagg ataaaccgcc atattcagcg cattcttaag
     8041 gacagagtga gtaatagtca ttgttttctt cgagtaaccc tttttaaatt cagcattgat
     8101 gaaattttgt aaaagagcag gggagagatc cgtaagtttt ttctttccta aataaccgtt
     8161 tatatgattt ttgatggtaa atcggtagtt ttcataggta ttgtatttta gatttagttt
     8221 aacgtattcc tccatccaaa aatcaaggta ttgttttact cgagtatccg tacctaaaaa
     8281 gtattgtcct gtttcgtcaa tatctgataa aacttttcgt aaagcagctt cggcctctgg
     8341 tcgggtgtct ccgccaactt tctccacttt ttttcttgag ccatcatcat tgatatcttc
     8401 gccgtttgta taattaagcg agacaaataa aaaagccatt tatgttacac tc
//
LOCUS       DAXLPI010000107         8340 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXLPI010000107 DAXLPI010000000
VERSION     DAXLPI010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.108"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..253)
                     /locus_tag="ACF3T5_002194"
     CDS             complement(<1..253)
                     /locus_tag="ACF3T5_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632757.1"
                     /translation="MKSKIILGGLLLSTTAFVSLQSPQITSAESLTNSAEIIVPLNLK
                     VKAHYTNGLPLEAGKEVRLRNLTDGSTEVLKKQVDANGEV"
     gene            complement(682..1872)
                     /locus_tag="ACF3T5_002195"
     CDS             complement(682..1872)
                     /locus_tag="ACF3T5_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HFD3632758.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFKGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGAFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(2458..2862)
                     /locus_tag="ACF3T5_002196"
     CDS             complement(2458..2862)
                     /locus_tag="ACF3T5_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632759.1"
                     /translation="MTNEIILMSHGLMAEEILNSAKMILGNQINYPVVSMTQNDGIEG
                     TINKLNKILEETSDIRNIIILVDLMGGTPANVAMMKASMDKRIQVVTGMNLGMVLESY
                     FKINEEKLAEHLIEIGKSSIKIPEIQMDGGEE"
     gene            complement(2877..3359)
                     /locus_tag="ACF3T5_002197"
     CDS             complement(2877..3359)
                     /locus_tag="ACF3T5_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFD3632760.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDTVANND
                     IQKQVLRMAVPAGISSSIITEEKAITNIKNGKYEGQNVLLIVKSPVDLLSFIENDLQI
                     DRINVGNMSNRKGTTVLRPNISVTNEERNAFEKLLNLGVEITTIMTPDDKKTYLKDIL
                     "
     gene            complement(3379..4191)
                     /locus_tag="ACF3T5_002198"
     CDS             complement(3379..4191)
                     /locus_tag="ACF3T5_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFD3632761.1"
                     /translation="MNSLETTKLGVNSKIKKSVLWRWFFTSSVSSNYEKMQALAYCYA
                     VLPFLKVTYKNKPEALQKAVLNHLQFFNTNPWVAPYILGINIAMEENSDENTEEAVTS
                     IKTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPVGIILWIAVSVALKMISIPL
                     FRIGYTSGTKLITTIEKSLKLLTESTSILGLMVVGALIPSVVKTNVVLDFKQGDFSMR
                     GQEILDQIMPGLLPALLVGLVYWSLKKNVKPIYLILGVMVLSIVLATLGILK"
     gene            complement(4184..4969)
                     /locus_tag="ACF3T5_002199"
     CDS             complement(4184..4969)
                     /locus_tag="ACF3T5_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFD3632762.1"
                     /translation="MEISVIQLGLIVLYGFFINFEKNSTMFGTYQPVTAGFVTGLILG
                     DIKTGLFIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTGQESSVGISIGIPV
                     ALLMVQLDIIRNTIGIWMVHKAEAGVKRGDYSMISKMQMFGVFLTAATTGIPVALAVI
                     FGPSLINTILAHTPEWLTNGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVMSVY
                     LKMPLLGVALIGAAIALIIYKKNSQNVINNTVVNTIGGMDEDE"
     gene            complement(4969..7044)
                     /locus_tag="ACF3T5_002200"
     CDS             complement(4969..7044)
                     /locus_tag="ACF3T5_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HFD3632763.1"
                     /translation="MKEVINIESNYFSRKFDDELLIIEAWGENAIRIRSFVDMNYEDR
                     QNSLIDKPNSLNKVIINQYENKTELINGKIRAVLDHRDRLTFYNEKNDVLLKEYIRLR
                     AVKHDDGGEDAGSVEVTKDFNSTLKLKSREYRSNMDGKFQVTTRFESDPKEKIFGMGQ
                     YQHSYLDLKNTVLELAQRNSQVSIPFYISSLGYGFLWNNPGIGEVSFAKNITRWKMYS
                     SDYIDYVIISGNEPKEILRNYSNLTGKVPKMPDSLLGLWQSKLRYRSPEEVMDVVEKY
                     HKRGIKLSTIAIDYFHWPKQGEYCFDEEYWPNPEKFLKTLKTDYGVEPIISIWPTVQT
                     DAENYEKYLENGYLVKVNRGVRLTMQIQGNTIFIDMTNSEARKYVWSLIKKNYVDKGI
                     NYFWLDVAEPGYSVYDFDNYRYKKGTDLYSGNLYPIDYLSMIYEELSNEQPVITLVRG
                     GWAGAQRFGALLWSGDIDSSFEAFRNQVNTGLNVGLAGIPWWTTDIGGFHGGNPEDPE
                     FRELLIRWFQYATFSPILRMHGDRLPHSKPLSDHGGGSMVTGAPNEIWSYGIEVEQIL
                     TKYIKIREALKPYISTLMDKAHRFGDPIMRTLFYEYPSDNNTWAVEDTYLFGDTFLIA
                     PIVNYKETVREVYLPINENWTNIWTGEEYDGGQTLDVEADISMIPVFIKTNQKDKFSK
                     LLSIIKEEI"
     gene            7352..>8340
                     /locus_tag="ACF3T5_002201"
     CDS             7352..>8340
                     /locus_tag="ACF3T5_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFD3632764.1"
                     /translation="MISSKYTIREQNEAKILNQIIRHKEISRAELSKITSLNKASVSS
                     IINKLIDDSLVIENRIGEATNRGRKPIMLTFNGVSSGLAIAIDLGYNYIDGMVASLDG
                     KEIARIQLTDTYVSKDNIQMLIRKIIKKLTDNLPYTRHGIIGMTIAVQGQVLNNKVLS
                     TTYNGLEEIDLVKLLNQEYSFPVFLQNEANLSALGEYTFSSDIENLVSISLHSGIGVG
                     VVKNGKLDIGNKGYAGQLGHTILFPNGRKCECGNYGCLEKYCSTQVIYQEISKEKKLD
                     KINSDIVEYLYSNKDQKVVEMIELYAYYLSIGINNIVMLYAPELVIFNSPLTK"
BASE COUNT         2679 a         1570 c         1163 g         2928 t
ORIGIN      
        1 ttacctctcc gttagcgtct acctgtttct taagtacttc cgttgatcca tctgttaaat
       61 ttcttaaccg tacttctttt cccgcttcaa gtggtaatcc atttgtgtaa tgtgctttca
      121 cctttaaatt aagcggtact ataatttctg ctgaatttgt taaactttct gcactggtaa
      181 tttgtgggga ttgtagtgaa acgaaagctg ttgtagatag tagcaaaccg ccaagaatta
      241 tctttgattt cataatatca ttctccttaa attctattta ttaagctcaa aaaatagttt
      301 ttatgttaat attctagcta ttaaaattct taatactaca aaaggaaacc attgttttat
      361 tgaatagaga catgtcccta tctcctctct aattagtttt tataaaacgt aactattacg
      421 ttttataaaa acgattttac aaatttactt aaaaaatgtc aataaaaatg aaggttcatt
      481 ttttattgaa gtgccaaaaa tagattaata taaaattcaa ctcgattttt ttcagatagc
      541 taaaaatgca acttcagctt tattagctaa aaagttgcat tttccccgta taataaattt
      601 tatagtctaa agcatttcta gaatttgatt gggagcgtca ataattttgt gtaaatgatt
      661 cttccctact gcagattgtt tctaattctc aatcagttgg cttagcattt gttcaagttc
      721 gccttcggcc tgttgaaagc ctttatgact gcgacctagg aatttttggt tgtaagtatc
      781 aaaagacgag actaagaaac gttccatcga ctcttcgttt tggaactgtt ccttgcggtg
      841 gctgtatttc ttgatttgct tattaaagga ttcaatcaag ttcgtagaat aaatactctt
      901 gcgtatggcc aaggggaaat catagaaagt gagcaagtga tcgttcgaaa gaatcgattc
      961 aaccactttt ggatagctgg ttttccattt ttccgcaaaa gcaccacgtg cttccaacgc
     1021 cacctcttta gatgaggctt gatacaccat tttaaaatca tcacagactt ccttacgatc
     1081 atcgacacgc actttgtgac tgatattacg ggaaacgtgt acacaacaat gttgaaaacg
     1141 agctttggga tagaaccgac taatcgctcc taccatcccc tttaagccat ccgtgatgaa
     1201 caggaggaca tttttcaaac cgcgctcttg aaggtccaat aaaatttcct cccaaatcgt
     1261 gatggattca gtcggtgcaa tcgcatagct caatacttcc tttgatccgt ccgggcgaat
     1321 gccaactgcg atatgaatag cttccttggc gacggttttc cgctttaacg gaatatacgt
     1381 tgcgtccata taaatagcag cataacggtc atgaagctcc cgccctttaa acgccgttac
     1441 ctcttctgta aatgattttg ttatattgga catggtttgg ggcgtgtagt aatgcccata
     1501 cattttctca atcaagtctg cgatttccga catggtaata ccttttcgga agaggtgaat
     1561 gacggtctcc tctaacgtgt cattcgtccg tctataagca ggaacagtct gttgcttgaa
     1621 ctcaccattg cggtcgcgcg gaatctggag atgaagttcc ccatactcgg tcttgaccgt
     1681 acggtcatag gagccgttac gagaattacc cgtgttaaaa ccaatgcgat catacttttc
     1741 gtaatctagg aaagccgtta actccgtttt gagaagcgta ttgactgctt tctccaaatg
     1801 gacacggaat aattcattca aatcgccttt gttcgctaga gtctttagaa tttctgtagt
     1861 aaaatcgttc atagggaagt cctctcttct gtgaatgtgt tgtggtaact ctattctaca
     1921 gaagggactt ccttttttct atttacacaa aatattttac actctcattt gattggacaa
     1981 cattcatata tatatcaaaa aaactttaac acaactatat aatatttgca tatagatcat
     2041 atcccaccgc tgttaagatt gttacatcta gaattagtac ctactctggc actaatttcc
     2101 taaccgtctc attgaaaata ccacttatac ttgaaagcat cttcaatctt agattgtcct
     2161 ccaaatagta cactcgaacc attattgctt tactatttaa ctatttggtt tttatagcag
     2221 ttcaagcact agctataccg tgctattcca aaaagagccc ttttaacatt cctctatttc
     2281 gctagtatta attatttaag attataatga aaaaaacata tttcttacta tggcttcgta
     2341 tctaattttg atattatgta gaaaacgaat catattaata tcgaaaatag atttcctttt
     2401 gttttgattt aaactttttc aattgagatt aacagaacta ttcttccttc attataatta
     2461 ctcttctcca ccatccatct gtatctcagg tattttaata ctacttttac ctatttctat
     2521 taaatgctca gcaagttttt cttcattaat tttaaagtat gattctaata ccatccctag
     2581 attcattcca gttactactt gaattctttt atccatactt gctttcatca tagctacatt
     2641 tgcaggtgtt cctcccatta ggtctactaa tataataata tttcgtatgt cacttgtttc
     2701 ttccagtatt ttattcaatt tatttattgt tccttcaata ccgtcatttt gtgtcataga
     2761 aactaccgga taattaatct ggtttccaag tatcatttta gctgaattta atatttcctc
     2821 agccattaaa ccatgactca ttaaaattat ttcattagtc atcgcactta ttccccttat
     2881 aaaatatctt ttaagtaagt ttttttatcg tctggtgtca taattgtagt aatttcaaca
     2941 ccaagattta atagcttctc gaaggcattt ctttcttcat tagtaacgct aatattagga
     3001 cgtaaaaccg ttgttccttt tcgattagac atgttgccaa cgttaatcct atctatctgt
     3061 aaatcatttt cgataaacga taataagtct actggggatt tgactattaa aagcacattt
     3121 tgcccttcat attttccatt tttaatattg gtaatcgctt tctcctcagt tataattgaa
     3181 gaactgattc ctgcagggac tgccatcctt agcacctgtt tttgtatatc attatttgca
     3241 acagtatcat ttacaaccat caaacgagtc acacctaatt tattactcca cattgtagct
     3301 acttgaccat gaattaatcg atcgtcaatt ctaatatttt ttataccctt catttccaca
     3361 aaaatttcct cctatcattt attttaaaat gcctagagta gctaaaacaa tagacagaac
     3421 catcacacca agaattaaat aaataggttt tacatttttt ttcaaagacc agtaaactaa
     3481 accaactaaa agtgcaggta ataatcctgg cattatttga tctagtattt cttgccctct
     3541 catagaaaaa tcaccttgtt taaaatctaa gacgacattt gttttaacaa cagatggtat
     3601 taatgctcca acaaccatta atcctaaaat tgaagtcgat tcagttaaaa gttttaaact
     3661 tttttcaata gtagtaatta atttcgtacc tgaagtatag cctatgcgaa ataatggtat
     3721 acttatcatc tttaaagcta cacttacagc aatccataaa ataattccta ctggactacc
     3781 atcaattgcc atatttgctg caatagcccc aaatatagtc catggaataa ctacaaatag
     3841 actatcacct aatccagcta ctggccccat aagtcctgtc ttaatcgatg tcactgcttc
     3901 ttcagtattt tcatctgaat tttcttccat agctatatta atacctaaaa tatacggtgc
     3961 aacccaagga tttgtattga aaaattgtaa atgatttagt actgcttttt gtaacgcttc
     4021 tggtttgttt ttataagtaa cttttaagaa tggtaataca gcgtaacaat atgctagagc
     4081 ttgcatcttt tcataatttg aagaaactga gcttgtaaaa aaccatctcc ataatacact
     4141 ctttttaatt ttggaattaa caccaagttt tgtcgtctcc aagctattca tcctcatcca
     4201 tacctcctat tgtattaaca acggtattat taattacatt ctgactattt tttttataaa
     4261 taattaaagc tatagcagcg cctattaatg ctactcctaa taatggcatt tttaagtaaa
     4321 cagacattac aaaaccgata acaagataac tgaaatattc ttttgcaggt aagtatcgaa
     4381 gcaataaacc aataccaaca gctggtaata atcccccagc aactgttaac ccatttgtta
     4441 accattcagg agtatgagca agaatagtat taattaaaga tggtccaaaa attactgcca
     4501 acgcaaccgg tatgcccgtt gttgccgccg ttaagaatac tccaaacatt tgcatcttgg
     4561 agatcatact ataatctcct cgctttacac ctgcttctgc tttatgaacc atccaaatac
     4621 caattgtatt tctaataata tctaattgaa ccattaatag tgctactggt ataccaatag
     4681 aaatccctac cgaagattcc tgaccagttg ttatggtgat aaaagttgcg acaatacttg
     4741 cagtttgata gtctggtata gaagctcctc caaaattact aattcctaaa gacaataatt
     4801 gtaaggttcc cccaataaaa agcccagttt ttatatctcc aagtattaaa ccagttacaa
     4861 atcccgctgt tacaggttgg taggttccga acattgttga attcttctca aaattaataa
     4921 aaaaaccata taacacaatt aaaccaagtt gaataacact tatctccatt agatctcctc
     4981 cttaattata cttaatagtt ttgaaaattt atctttttga tttgtcttta taaaaacagg
     5041 tatcatacta atatctgctt caacatccaa cgtttgtccg ccatcatatt cttcacctgt
     5101 ccatatattg gtccagtttt catttattgg taagtaaact tctcttactg tttccttata
     5161 atttacaatc ggtgcaatta ggaacgtatc tccaaataag taagtatctt ctacagccca
     5221 agtattatta tctgaaggat attcataaaa tagcgtcctc atgatcggat ctccgaatct
     5281 atgagcttta tccatcaaag tactgatata cggtttcaac gcttcacgta tttttatata
     5341 cttagttaat atctgctcaa cttctatacc gtatgaccat atttcatttg gtgcaccagt
     5401 aaccatcgaa cctccaccat gatccgataa gggtttacta tggggtaatc ggtcaccgtg
     5461 cattcttaaa attggtgaaa aagtagcata ttgaaaccaa cgaattagca attctctaaa
     5521 ctccggatct tctggatttc cgccatgaaa cccacctatg tcagttgtcc accagggaat
     5581 ccctgctaaa cctacattta gtccagtatt aacttgattt ctaaatgctt caaaactaga
     5641 atcaatatct ccagaccaca ataatgcacc gaatctttgt gctccagccc atcctcctct
     5701 aacaagagta attacaggct gttcattact taattcttca taaatcattg aaagataatc
     5761 gattggatat aaattaccac tatataaatc agtccccttt ttataacgat aattatcaaa
     5821 gtcataaacg gaatatcctg gctcagctac atctaaccag aaataattaa tccctttatc
     5881 aacataattc tttttaatta gactccaaac atatttccga gcttcactat ttgtcatatc
     5941 aataaaaata gtgtttcctt gaatttgcat ggttaatcga actccacgat taacttttac
     6001 tagataacca ttttctaaat atttttcata attttctgca tcagtttgta ctgtaggcca
     6061 aattgagatt attggttcca ctccgtaatc agttttcaaa gtctttaaaa atttttctgg
     6121 attaggccag tattcctcat caaaacaata ttctccttgc tttggccagt gaaaataatc
     6181 aatagcgata gttgaaagct ttattccacg tttatgatac ttttctacta catccatcac
     6241 ttcctctggt gatctatatc gtaatttgct ctgccataat cctaataaac tatctggcat
     6301 tttagggact tttccagtta gattactata gtttctaagt atttcctttg gttcatttcc
     6361 tgaaattatt acataatcga tataatcaga agaatacatt ttccatcgag ttatattttt
     6421 agcaaaagaa acttctccaa taccaggatt attccataaa aaaccgtatc caagactaga
     6481 tatatagaat ggaattgaca cctgtgagtt tctttgcgct aattctaaaa cagtgttctt
     6541 caaatcaaga taagaatgtt gatattgacc cattccgaat attttttctt ttggatctga
     6601 ttcaaatcta gtagttactt gaaatttacc atccatattg gaacgatatt ctcttgactt
     6661 taatttcaat gtagaattaa aatcctttgt aacttcaact gaaccagcat cttcaccacc
     6721 atcatcatgt ttaacagctc ttaatctaat atattctttt agtaagacat cgtttttttc
     6781 attataaaaa gttaaacgat cacgatgatc tagtaccgct cttattttcc cgttaattaa
     6841 ttctgtttta ttttcatatt gatttattat cactttattt agggaattag gcttatcaat
     6901 taaggagttt tgtcgatctt catagttcat gtctacaaat gaacgtattc taatagcatt
     6961 ttccccccat gcttcaataa tcagtaattc atcatcaaat tttcttgaaa aataatttga
     7021 ttcaatgttt atcacttctt tcatcccact ttccttttct tcttaattag ttaactctta
     7081 ttacaacact agcaattgta atcgcataca tatacatatc aatttaattt aagtattttg
     7141 actagaattc tgctttttaa tttgttttct cgataaacta attatcaaaa aaaaaactaa
     7201 taacaaaaaa cacttaatta cttgaactaa aattgtttgt acgttaaact aattaataaa
     7261 taaaatataa aataagctat ttaaaatgtc aactgtaaac gctatatttt ttttgcatag
     7321 gctattttat ttttaaattg aggagaaatt aatgatttca agtaaatata caattagaga
     7381 acaaaacgag gcaaaaatac ttaatcaaat aattagacat aaagaaatat ctagagctga
     7441 attgagtaaa ataacctctt taaacaaagc atctgtatct tctattatca ataaattgat
     7501 cgatgactcg cttgtaattg aaaatagaat tggagaagca acaaatcgtg gccgaaaacc
     7561 cattatgtta acttttaatg gagtatcttc gggcttagca attgcaattg atctaggata
     7621 taattacatt gatggaatgg tagcttcatt agatggaaaa gaaattgcaa gaatacagct
     7681 tactgatact tatgttagta aagacaacat acaaatgtta attcgtaaaa ttataaaaaa
     7741 actcaccgat aatcttcctt atacgcgaca tggaataatc ggtatgacaa ttgctgttca
     7801 aggacaagtt ctaaataata aagtacttag tacaacttat aacggtcttg aggaaattga
     7861 tctagtaaaa ttattaaatc aagagtatag ctttcctgtt tttttacaaa atgaggcaaa
     7921 cttatctgca ttaggtgaat atactttttc ttctgatatc gagaatttag tatcaattag
     7981 tcttcatagt ggaataggcg ttggagtagt aaaaaatggt aaacttgaca ttggtaataa
     8041 aggatatgct ggtcagttgg gacatacaat tctatttcca aatggcagaa aatgtgaatg
     8101 tggtaattat ggttgtttag aaaaatattg ctctactcaa gtaatttacc aagagatttc
     8161 caaagaaaaa aaactagata aaattaattc ggatatagtg gaatatcttt atagcaacaa
     8221 ggatcaaaag gttgtagaga tgattgaact atatgcatac tatttatcga taggaattaa
     8281 caatatagtt atgctctatg ctcctgagtt agtgattttt aacagtcctt taacaaaaaa
//
LOCUS       DAXLPI010000108         8338 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXLPI010000108 DAXLPI010000000
VERSION     DAXLPI010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.109"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..425
                     /locus_tag="ACF3T5_002202"
     CDS             <1..425
                     /locus_tag="ACF3T5_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632765.1"
                     /translation="KFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGT
                     LIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLP
                     PKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            520..1404
                     /locus_tag="ACF3T5_002203"
     CDS             520..1404
                     /locus_tag="ACF3T5_002203"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFD3632766.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1628..4795
                     /locus_tag="ACF3T5_002204"
     CDS             1628..4795
                     /locus_tag="ACF3T5_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFD3632767.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4863..5360
                     /locus_tag="ACF3T5_002205"
     CDS             4863..5360
                     /locus_tag="ACF3T5_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFD3632768.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            5379..5843
                     /locus_tag="ACF3T5_002206"
                     /pseudo
     CDS             5379..5843
                     /locus_tag="ACF3T5_002206"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            5840..6598
                     /locus_tag="ACF3T5_002207"
     CDS             5840..6598
                     /locus_tag="ACF3T5_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFD3632769.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6684..6998)
                     /locus_tag="ACF3T5_002208"
                     /pseudo
     CDS             complement(6684..6998)
                     /locus_tag="ACF3T5_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
     gene            complement(7051..8223)
                     /locus_tag="ACF3T5_002209"
     CDS             complement(7051..8223)
                     /locus_tag="ACF3T5_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HFD3632770.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         2708 a         1458 c         1775 g         2397 t
ORIGIN      
        1 gcaaatttct tttatttcta gaagttattc acgtatacgg atttgttttt ctcacaaata
       61 aaaaggcagg aagtacaatg gatcatttgt atataaaata tattcatcaa ttttccgttt
      121 tggtgttagg cacactaatt atagcgggga ttgtcgcatt tattattcaa ggccgtgaca
      181 tattaacagt acatcaagag tttttcagtt atgcatttgg tattcctctg atcgctataa
      241 ggtctgataa caaaaagtgg aagaaacgct attacattct cttgctattt ttgtgttttt
      301 atctgccacc taagattttt actttattag gtatacaatg cctatcattt tcttttattt
      361 cctatatcat tgttttaatt ttcctcagtt tcatagtatg gatgttttat cttgtcaaaa
      421 aataaatgaa tgtttcatga acagtacttc atcacagcct aatttttttc tttatgctat
      481 cgttatatta aagaaatcaa tcagaaggga aagaaagtga tgaaaaaagt tttattagtg
      541 gtaaacccca gttcaggcgg tgaacaagca aaggaatttg agcaattagc gatagcaaaa
      601 ttggaatcgg tgtttgatga agtggtcgtc ttgcatacaa aaaaagcagg ggatgcaaaa
      661 aactttactc gcgaagcggc tacggaaggg tatcatagtg tgtttgtcat gggcggagat
      721 ggaacagtca acgaaggaat cagtggaatc gctgaacaag aacatcggcc aaatttcgga
      781 tttttcccat tgggtacggt gaatgacttg gctcgagcat tagggatacc gctagaacct
      841 gaggaagcga tcaatcattt ttcgattgaa tcagtcaagc cattggatat tggaaaaata
      901 aatgacgact attttatgaa tgtcgtcgca attggctcta tccctgaatc aatcaatgat
      961 gtagatgcgg agaagaagac gaaatttgga aaactagctt atttcatgtc aggaatcaaa
     1021 cagttagctt ccacacaatc ctactcgttt cacgtagaag ttgatggaaa gaaggaagaa
     1081 attgagagca gtaccttgct gataggacta actaactcag tcggcggttt tgaaacatta
     1141 cttccaaatg caaaagtgga tgatggtaag ctccatttgg tttatttaaa agattcttct
     1201 ttgatcgata cgcttaaaac attgccagac ctattaaaag gagtagatga atctacgaaa
     1261 aatctagcct accttacttg tgagcaatta gttgtgtcat tagctgatga ggaagaattg
     1321 acgacgaatg ttgatggaga tgaaggggat aaattacctg tgacaatcaa agtattgccc
     1381 tcacatttac aggtttattg ttaaaatgga ggttgtgacc gaggagttgg cgggagaaca
     1441 ccgcttgttc gggataaggg actgtgacaa gacttttgtc acagcctttt ttttgtacaa
     1501 aaaagaaatt tacacctaat agaaaaattt ttccataaaa atacatattt taaatatgaa
     1561 agtacaattg gaaatagatt aaaacgataa aataaaattg tcaaaacgtc agagaggatg
     1621 gtggagaatg aatcaatgtg taaaaaagct gattgcgtcg attttgtttg tcttccctct
     1681 cttgatggtg acatccgttt ttgccgatgt agagaaaaca aacgaatcaa cgcaatcaga
     1741 gaatcaaagt tttgacaaga ctactttttc agaagaatcg attacttctg aaactacaga
     1801 atctcaaagt gaggcttcta ctaataaaag gcaagaagaa aataagacaa gtgagacctc
     1861 agaagtagct gaacaaacgt ctgatagcag tgaagcggca ctggcaagag ctgtttcagc
     1921 ggatgggtac agcgaagctg ccacaacaga agaactggcg cagcttctcg cagacgaatc
     1981 agtaactaag atccgactga ttcagccttt gactttagac agggaattag agatcaaacg
     2041 agatatcgtt attgattttg gtggattcgc tcacaatttc ggcacacacc atatttatat
     2101 caatgagata cctcagtcga tcgagttcca gaattttaaa ggaacagctg cacatccagg
     2161 tggattgaca ccaaatatcg atggaaatgc cattatcatt gcttatatga ccgatttttg
     2221 gggaagcaga tatcatttta cgggagaaat aaaatttacc gggacacttg atttgtatga
     2281 tgggtccaaa ttaggactga tttacgctcc tcgtgcaact gtcacattag atggtgtttc
     2341 cggtgtgttg gatgtccaac cagtgaagga aggactgaat gccgctccgg gtaaagccta
     2401 tttttgtaga agctatcaat tgaatgtgat caatggtagt caactttatg gaccgtatct
     2461 agggaaattc tacggttttc tagattcagg agatgagact ggtagtggaa aaacagcgcc
     2521 aggaatccat atcttaagcg gttcaaaggt ctctcttgat tatgatcggg cagatggcgt
     2581 tagcgatgaa ggagaggcag tcgatacgct acctggtaat gtcatgtttg aagtcagtgg
     2641 ttcgggaagc gaattttcgg taaatacaaa gatcaatatt accaatgaca acaatcgcgg
     2701 aatcatccaa atgagaggtt caggatcgaa agtcactgta tcgaatggtg ggaaaatcac
     2761 catcaataca gcaactacgg gcggatttcg cttgcaagga gattctagtc aaatccatgt
     2821 atcaagtggt ggacaaatct cggtcaaaat ggctggagat gacgatcaac ccgggaacaa
     2881 tgggatgcgt tttgtaggaa aaggcctttc tttgatcgta gaaggagccg gatcagttat
     2941 cgacatcgat aagcagagtg ggcgttccag cgcagttcgt tttgagaatg gtacgcaaaa
     3001 gctgacagta accaatggag gctcattgag ggtaagaaat acaggtgatg gcaaatctta
     3061 cgtgaacaga ctcaatcaag gaaatcaagc gatccaattt tatgacgctt ctggttttct
     3121 agaaagtcca ggttcagctg attttacagt cactggagaa aaatcaaata tcgatatacg
     3181 cgcagattcc ggagcaactg tagatacaac tggagatatt gatttaaatt ttacggcagg
     3241 tgaaaaaact tatttagtca tggtcgggaa aacaggggaa gataagaccg gcatcatcag
     3301 tggggatgtg ttgaatgtgg acttagaatc tcctacctat tttgatttcc aaaacaaacg
     3361 gattggaagc gagaccaatg gcataggcgg ctgggtattc caaggaaatg gagattcttc
     3421 tttaaagatc gcccattcag aaatcgcttt atggcgaaaa acaggcgcaa cattcgataa
     3481 ctttgaaaaa gacccggatt atttttctga tatgacagac ttatcgatca atgggaccga
     3541 cttaggcaat ttcgtatcaa gccctagtgc aggtttaaca aatgaatttg ctcagacagg
     3601 tgccggaatg aaaaattata atcgaatcag tgccaataat cagctcccga tgattgaatc
     3661 cttaagagtg ccaacgaatg cagataaaaa aatctatggg catgtcgttg ttcccgaagg
     3721 tgtcgaggca aagccaagag atgcttggac aggcgaagta gaagtgactt tacgtattac
     3781 ttacgcagat acgacgaaat catctgtaga aattactgct ctcacgcaag gacaaacaga
     3841 tgaaaatgaa ggggcaggat ataacccatg gggagaaggc gaacaaggcg gcttgttcga
     3901 agttcaagta cccaatgatg agagattaaa aacggaggat caagtgcaag ttgttgctgc
     3961 taaaaaagta agaggtggcc aagaagtgac agaactggac tctgccaaaa cgacagtcga
     4021 tgtgattcct cctgaacctg ctaatttaag taccacattg atcaatgcag cttctcgcac
     4081 gatcacaggt acaggtgaag caggagcctt ggtttcattg aaaaaaaatc aagctgtttt
     4141 gtcagaaaca actattgatg atacagggga atttgttctt tctatcccag aaaatcaact
     4201 ttttgcagga gatgtattag aaattgtatt aaacgaccaa gcaggagaag ctactgctaa
     4261 gggtgtgcag aacaagccaa gtaccaacga tgaaattggg aatcacaatc caagtgatac
     4321 gccagttgtt tatcatgatg ccccgccttt tgcacctgct acgaagatcg tagtagaagg
     4381 tggtctctca tttcttgccc cttccaaact cgattttggt cagatcaaag caactggttt
     4441 gacacaagaa gcatttggcc aaatggatat acaggaaaaa ttatggattt atgatcagcg
     4501 agaaacgaaa aatccgtgga tcttaacgat aaaattatct cagcctttca caaatgatgc
     4561 aggattttct atgcaggatg tcctatattt caagaaagga gaggagtatc aattagtgaa
     4621 tgaagaagaa ctggaaattg tcagaggaca aaattcaacg aatgtgcaag cagattattc
     4681 agaatattta aataacggtc gggcgtttaa acttgtctta accaaagaaa atcagatagc
     4741 tggtaattac tcagcagaga taacatggac attagcagat gcgccgacag aataaagatc
     4801 aaggaggatg attgatgaat cgaatatgga ataaaagtca tcgaaacaac tgctctttac
     4861 ttatgatcct tctccttgga ttcatccttt ggcagacacc tgtccatgcc tccgaattca
     4921 attttgcggt agattttgaa ttaccggcta atcagattga taagaacaaa acatatataa
     4981 atgtaaggat ggagcctggg aaaacacagg aaatccccta cacggtcttt aaccgaacag
     5041 ataaatcagt aacagttgag acattgatcc actccgctac gacgaattca aacggtgtga
     5101 tcgaatatgg agaatcaaaa agtaaatttg atgaaacatt aagttatgat atgaaaaact
     5161 tagcaaaaac agtgggagaa ataactgttc ctcctcaaag tgcggtcact gagacacttt
     5221 cactgaaagc gccagaaaaa gagctggctg gtgtggtcgc aggagggatc acgtttcaag
     5281 aaaaaaaaga gaacgtttct aacaaaagta cagttggtgt cgtcaatcag ttttctcaag
     5341 tgaaagcaat cttgatttga aatagcgaaa aagagtcttc cccagatttg aaattaaaaa
     5401 aagcttatgc ggatcaaagc gatggaagaa atgtgattaa ggcgaatctt caaaatccaa
     5461 atgcttctta tatgttcggc ttgactacat cggttgaaat ctacaaaaaa gaaacaaaag
     5521 agccttacct ttcaataaaa aaagaaaaaa tagatgtagc acccaataca aattttgatt
     5581 tacttgtccc attagaagga aaaaaattag ctccgggaca ttatcaagcg gttgtgaagg
     5641 gtgcttggaa agaaaaacgc tggaatctcc ataccgattt tgaaataact gatgatgcag
     5701 cggaccaatt gaatgaaaag gatattgatt taaaaaatca gccagaaaca tttgactggc
     5761 gatggctgat acttgttgga gtcattctta ttggcttatt tgctggatta tttattttaa
     5821 aagaaagaag gaaaaagaaa tgaaaaaaac aattgcatta agtaccgttc tagctggaag
     5881 tctattatta tgtacacaaa gtgtttttgc tgagaataag agctcagcca ctacagaagg
     5941 aacgatttct gtgaccaaac cagatgctgc aaatcctgaa gacgttgttc cagttcctcc
     6001 tgttgatcca aatgatcctg atactccttt tacacctgac gaaccaaatc agggaacatc
     6061 cggattactg acgattgacc aagctccaga tcttgatttt ggaacaatca agttaggtac
     6121 agcaaaaaca gtatatgctg cattacaaag cggaacggac agtactggag cacaaacaca
     6181 agtacagaat ttcgtacaag taacggataa aagcggaaat tatgccggtt ggaaattatc
     6241 agtcaaacga acagaattta ttggtacaac atctgatacg aataaactaa ctggttcaaa
     6301 ggtgaaattc aaaaacgccg tgattcgaaa tagcgcaact aatagcagtg gaatgccatc
     6361 taccgtttct ggaactggcg ctgaaggaat cgaaatccca gcagatcagg cagtagatct
     6421 agtaactgca ggagcaaatg aagggattgg cacatggatt tattcattag gagagaatgt
     6481 ggaacaaggg agacaatcag tggaattatt tgttcctgta tccaattatg ctgtagacaa
     6541 ctacgtttca acattcacat ggtcgttaac agatgctcct acagaagagg aagcgtaaaa
     6601 tcatatcaac gacttcaggg agtggagccg attcaaggcc cactccctga aatctgctat
     6661 gcttacgtct cagctcttat tcatcaactc gaagcagttt tttggaagtc atggaaacgt
     6721 tcctttgtct tttcaaagat ctttcgacca acgagtaagg agtcaccgtt gaaaaattct
     6781 aacacataat gagcagaatc aattttctgc aattgcgata agttgataca gtaagatttt
     6841 aaaagtaaaa agtaagcata cttttctaac tctgtcttca attgttttaa agaagtattc
     6901 actaatttat cttctgtagt tgttttgacc ataacttggt ttttcaaacc tttgatactt
     6961 tcaatatata aaatttcccg aggatacagg acgagacttc agtcaagtcc agactcctgt
     7021 gtaaaatgct atacaatgtt tttaccattt ctacttatca aaattgatgt attttcttga
     7081 agaataaatc cattcatcat gtaggtccat aagaacggct ccaattaagc gattggctga
     7141 tgtttgattg gggaagatgc gaataatctt ttctcttctg cgtacttctt gattcagtcg
     7201 ttcaattaga ttggtactct ttagtcgatt gtgggaattt ccttgtacgg tatattgaaa
     7261 ggcgtcttcg aatccatcat ccaatgatgc gcaagctttt gaatattttg gttgatcgat
     7321 ataatcatga atcaatcgat ttttagcctc acgcgctaag ttaatatctg tgaacttaaa
     7381 aattccttta acagcttctc tgaaagattt tgaatttttt ttaggaatgg tggtaaagat
     7441 atttcttagg aagtgaactt ggcatctttg ccaacttacg ttggtgaagg attttctaat
     7501 ggcagagact aatcctttgt gcgcatcaga aataacgagt tccgtacctt gtaaaccgcg
     7561 ttcttttagg tattcaaaaa atgttgtcca ggtctcttcg ctttcgccac tttgaatcat
     7621 gaagccgata atttcacggt cgccatcttt ggttattcca atcgctatat gacagctttt
     7681 tgagagtact cgattttctt ctcgtacttt tatatagagt acatcggtca ttaagtaagg
     7741 ataatttttt tctgataata aacgattctg ccactcgtta accataggtt ctagctgttc
     7801 tgttaagcta gaaacgaagg acttagagac ggatttacca caaagttctt ccacaatttt
     7861 tgatacttta cgagttgaaa cgcctgatac atacatttcc aacattgaag ccatgagggc
     7921 tttttcgttt cgttgataac gttcaaacac tgtgggtgaa aaatggccat cacgtgttct
     7981 gggtactttt aattctagcg tgcctacacg tgtcgtaaag ctgcgctcat aatagccatt
     8041 tcgttgactt tgtcggtttt ctgttcgttc atattctttt gcttgaatat attctgttcg
     8101 ttgattttcc attagttgat taaataccgt tgttaaaata tttttagaaa cgtcatcctt
     8161 tacagaatat tcaataatgc tttgaatctc ttcgcttttc agtgtaaaat gtacttgggt
     8221 catgtaaaag tcctcctggg tatgtttttg tcgttaaaaa cattgtaccg taaaaggact
     8281 gttatatggc ctttttactt ttacacaatt atacggactt tatcggtttt tgaaagcc
//
LOCUS       DAXLPI010000109         8329 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXLPI010000109 DAXLPI010000000
VERSION     DAXLPI010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.110"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..2196
                     /locus_tag="ACF3T5_002210"
     CDS             <1..2196
                     /locus_tag="ACF3T5_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFD3632771.1"
                     /translation="VANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPG
                     GFDGVYTPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRK
                     MQQWLNVNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTY
                     PVSEGNSGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIAD
                     VVVIKGLLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDER
                     NKYLTTEELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTT
                     VIYFAVDLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFA
                     FVSDMSTGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSND
                     TNNDNYIAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPV
                     TEHDRKIYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVS
                     DFAGWLGDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAK
                     RNSDSTALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVG
                     IISFFIDKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            2276..2671
                     /locus_tag="ACF3T5_002211"
     CDS             2276..2671
                     /locus_tag="ACF3T5_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632772.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            2996..3613
                     /locus_tag="ACF3T5_002212"
     CDS             2996..3613
                     /locus_tag="ACF3T5_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3632773.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            3654..3953
                     /locus_tag="ACF3T5_002213"
     CDS             3654..3953
                     /locus_tag="ACF3T5_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HFD3632774.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            3987..4154
                     /locus_tag="ACF3T5_002214"
     CDS             3987..4154
                     /locus_tag="ACF3T5_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFD3632775.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(4199..4804)
                     /locus_tag="ACF3T5_002215"
     CDS             complement(4199..4804)
                     /locus_tag="ACF3T5_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3632776.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            4929..7916
                     /locus_tag="ACF3T5_002216"
     CDS             4929..7916
                     /locus_tag="ACF3T5_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFD3632777.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            8065..8274
                     /locus_tag="ACF3T5_002217"
                     /pseudo
     CDS             8065..8274
                     /locus_tag="ACF3T5_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346508.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
BASE COUNT         2827 a         1284 c         1570 g         2648 t
ORIGIN      
        1 gtagctaatt ttggacctac cactgaaaaa atgtacgata atcaggttac tcctaaatgg
       61 ggcaaaaatc tacctaaaaa tattgtattt cttattcaag gtgccttttg gtgtaagggg
      121 attaatccag gtggcttcga tggggtatat actccatatt tagatactgc tgtaaaagag
      181 ttgcagactg atgcagggtt taatggaagc gctgtaactg gagttttgga tgcgaaatgg
      241 gccaaagcgt tatttgatat gagtgctttt gttcttgttc ccggcggaga tagtaaaatt
      301 agaaaaatgc agcaatggtt aaatgtaaat tatttagaat acacgggaat aatgccttgt
      361 gatggtattt atcaaagaaa ttcaaatcaa gcattaattt ttgcattaca agctgaacta
      421 ggatattctc ccagtgaagc aacagggtct tatggtaatg ggacaactag taaaacttat
      481 cctgtgtctg aaggtaactc gggaaactat gtaagaataa ttcaatatgg attatatgtt
      541 aatggttatt ttgaagacgg tacattcgat ggtattttca ctaaatatat gggtttagaa
      601 gtactggcat tccgaaaatt tatggtattg cctgaatata ctgaaatagc agatgttgtt
      661 gtaataaaag gactattgtc tagcgctgga gatattagaa gatctgcaga tggcgcagat
      721 acttctacgc aattaactag atctcaaatc caaacattgg ttgataatga tataaaaata
      781 gttggtagat atcttactgg tactgttgga attggaaaag atgaacgaaa taagtatcta
      841 actacagagg aattgaataa tattttttct aaaaatttat ctgtgtttcc tatctatcaa
      901 gatggaggtg cagcattagc ttattttact tatgatcgtg gcttatcaga tggtaagaag
      961 gctatagatg cagcaaaaaa tctaaatata ccattaacaa cagttattta ttttgctgtt
     1021 gatctagaca tgttagggga agaaattctt gcatatgctg gagaatattt taagggggta
     1081 tctgctgtaa tgtcatatag cgggtatcaa acgggtgttt acggaacaag aaatgtttgt
     1141 tctcaaataa tcaataacgg gtatgcttcg tttgcatttg tttcagatat gtcgactggt
     1201 tatagtggga atttaggttt tcctatgcct agacaatggt cttttgatca atttgttgaa
     1261 tttaccatag gaagtggcaa cggagctgtt ggtatcgatt taatcgctac atcgggtaga
     1321 gatagcggat ttaatgagtt atcaaatgat actaataatg ataattacat tgccaaatac
     1381 aatcagaagg ttataaatca gatggtacgt gcgtatcaat atctatcaca agctggtctt
     1441 gataatcctt ggaaccctca tttgacattt tatagatatg taaattattc tggtttgtca
     1501 tgggacatca tttcttctcc tgtaacagag catgatagaa aaatttatga tgaatatagg
     1561 aataaattaa ctaatagcga gggcctgtat aattatttta tagatcctaa tactggttca
     1621 ataataggtt tgcctcactt aattgtgacg ttacaatcac agatgtttct agccgacaca
     1681 atcaatgata gtgtttctga ctttgcaggt tggttaggag atttaatgac ttgctggggt
     1741 gaagttaaga aattaggaat acaaatggag caaggtgtat ttgcattagt aggtacggct
     1801 gcaggtggaa catttagtct agaagattta taccaagatg ttgacgcggt aaatgtttat
     1861 accttagcaa aaagaaattc tgattcaaca gctttatcgg ctttaaaaac gtactattgt
     1921 gataagggtg ctattgatag atttaatcaa tttgttaaga acagatttgg ttcttcagat
     1981 aaaatcgctt cagtaacaaa tagtttatta gaaaatgacg aaacagttga tatcttaggt
     2041 gtagggataa tttcattttt cattgataaa atagttaata attcacttgg gacagatttt
     2101 acttatcttg attttattgg agatgagggg aaagttgcaa atggattttc tgaaaaaatt
     2161 attcaatttc aagaaaatag aagtaaactt aattaagaat cgtttactat ttaaacagtg
     2221 tccttttcct ttttaaatat atacgataaa ataagtatat gaattgtaaa aggacatgat
     2281 gattatggaa gaaatagaat ggaataatct gactcaaaat aataaaaata attctggatg
     2341 tggaattatc tttatgtttg ttatagtttt gggaatagta gtacttttaa taactgtcaa
     2401 gatagtagat tactttgcga atactcctga agaacaaata cagagaaaac ttcctgagtt
     2461 tgaaagagta ccaccttatg atccaagaga taattcattt caagtaaaaa aattaaaaga
     2521 gtctccaaat gaaatgtggt ttgaagttac agttgaatca agcgacttca aagaaaaaaa
     2581 gaaagaatac aattatattg taaaggcaaa agtatcaaaa agatttattt ttaaccctaa
     2641 attagaaata tatagtgttg taatagaata atagtgttaa aaaaattgta aaagttaaag
     2701 aagtcaaatt ctgatgtgaa aaataaaata taccatggaa gacataaaag aaaaactgga
     2761 taactagttt atttaatggc agaccgttaa ggcctgccat ttttatgtat tttagcggaa
     2821 taaatataat catgcgggag tgtaaactta ctgtttcaaa aagtatactt tttgacatgc
     2881 cttgattttc tttgtttatg tatggcataa taaccacata atacaatttg aaacgtttct
     2941 gatttaatct gattgttttg aaacaatttt agtgaaaaaa gggtagataa aaacgatgag
     3001 aaaatttttt tatatccggg tcagtagtta tgatcaatcg atcgaacgac aactgatttc
     3061 cgcgaaggaa ttaggaatcc ctgaagaata tatatttgta gaaaaagcca gcggcaaaga
     3121 ttttaaacgc cctgaatatc aattaatgaa acggatgttt cgaaaaggag atttactcta
     3181 tctacaatca ttagatagac taggcagaaa taaacaaatg attttagaag aatggcgaga
     3241 attaactaag gaaaagcaga ttgatatttc tgtgttagac atgcctctgt tagatactgc
     3301 tagttacaag caactagatg ggttagagac gcttatctct gacttaattc tgcaattact
     3361 cagctatatg gccgaagacg agcggaaacg aattaatagc cggcaaagag aaggcgtaga
     3421 agctgcgaaa caaaagggtg taaaattcgg cagaaagaaa attgctttac ctcctgaatt
     3481 tcctgcgatc tattctgact ggaaagcagg aaagataact gcagtctatg ctatggatca
     3541 attgggcatg aaacgaaata ccttttatcg acgggtgaaa gaatatgaac ataccttgca
     3601 ccacttttct tgatagatat aacagctatt agacgggaag ggagagtttg aaaatggact
     3661 taacgaatca aagggtcgtt tttactggaa cgctgcagca aatgacacga acccaagcca
     3721 tcggtttggt tcactcttta aacggccatt gtcaatcaag tgttacttca aaaacaaatt
     3781 tcctggtttg tggtcagtat tctaagtcgt tgtttgatga ttctctctat acaaaaaagg
     3841 aacaaaccgc gcgggacttg attgcgttag gtcatgagat taccatttta tcagaagtcg
     3901 aattttatgc gttaattagt caacagttga aagaatggca acggtattta taatcgttag
     3961 taaaatttac aaggaggaaa atttttatga agcgaaaatc ttatgatcgt tggggcattg
     4021 aaataactag ttttaatcga aatattgctt ttcacgtgga gtgtgactgt ggggaattag
     4081 caaaaagtgt ttatcaaaaa aattactttt actgtcctaa atgccatcgc cgatacgggg
     4141 tacatggaac atagaaacga aatagtgcgg tagaaaagcg aatgaatgta gtatatttct
     4201 acaaatcatt cgctttcttt attttataaa tagttggtcg agagatcccg ttttcttttg
     4261 caatctctgc aactggaatg ccttgcttta acatgtccac cacggtttta aaaactaagc
     4321 gtttttgcgg atctgttgag ttaatagaat actcgattgg tcgtccctta tatcggccgg
     4381 ctttttgagc caataatact ccttgccgct gacgttcacg aattttctca cgctcgtttt
     4441 gtgcaatata acttagcagt gataaaaaca tatcgaacat agcggtatcc aggagttcat
     4501 ttccagtatt aaaatctaaa aactgtgcat ctaatatttt taacgctact tttttctgtt
     4561 tcataaaagc aactgtattt tttatatctt cataatctct tcctaaacga tctagtgatg
     4621 taacgatgac gcaatcacct tcacggatat aatttagtaa tttttgaaac tcaggacgct
     4681 cagtatcttt accactggct ttttcttgga ataatttatc aatgttaagt ccttccatca
     4741 ttgccaattg tcgatccaaa ttttgatctt gtgaagatac tcgaatatag ccaatttttc
     4801 ccataaaaaa ctccttttta aaagtgatat aatttacatg taaaatttac ttgtagtttt
     4861 actgctatca tagcattttt aaaaataaat tttatgtaga cttaaaattt acttcatgga
     4921 gggtttaaat ggatgaaatc aaaagtaaca aaaaagcatt tggcatttat ttaggagaac
     4981 ctagtacaga acaattaaac caattttttt atttgcatga aaaagattta gaattgatgg
     5041 agactatacg acgaccagct actagattag gttttgcact acagttagtc tcactacgtt
     5101 ttttaggaac ttttatatta gatcttacca atattcccca aaatgtattg agttatgtag
     5161 caactcaact aaatattgat cctggagaaa ttatctacta tagcaggaga cagaccaggt
     5221 atcagcacac aaaattgata aaatcacatt atggatatca agatttcaac caaccggaaa
     5281 tagaaacata cctagctaac tggcttttaa atagagcgct gtatacaaca gaatcttcag
     5341 atatgttgtt tgatatgctt ctaaaaaaat gtctggatga aaagattgtt ttacctggaa
     5401 ttactacttt ttctcgtttt gttgctagtg caattgaaaa agctgaagag catttatacc
     5461 gacaattgtc tttggttcca actgaagatg agagaaagcg gcttctcaat catttagaat
     5521 taattggaac tccagtttat ggggcaataa tcaaaatgga cattttgaga accccgttag
     5581 ctgacgatag tcataaagaa atagccagag gttttgaccg actaaagcaa tttcaagctt
     5641 tctctactga aaaatggaag atggaatcta ttcctgaagg gaaaataaaa gttcttgcaa
     5701 attatgcatt taaagctaaa gctcaattaa ttcaacgaat gagtgaacag aaaaaactgg
     5761 cgttgttagt ggcatttatt ttcatataca aacgtaaggc tatggatgaa caaattttag
     5821 cactatctaa tttttttgaa actgttttta gacgcgccaa aaataaggaa tctaaagaac
     5881 gtcttcgtac cataaaagat ctagatcgag ccgcatcgac actttcagat attgtagatt
     5941 tagtaatgtc cagtcattct gatactaatg aactatccct tcgcgatcgt atcttagaaa
     6001 aatattctga ggaaaatgtt accgaagctg tatcgcaagt tcggctattg gttaaaaatg
     6061 atcgtgaacc aatagccatt tctgaacttc tgaacactta tcgaaagttc agaaaattta
     6121 ttccggatat tttaactaca ctaacatttg acagcaatga ctacggtaac aactgtaagg
     6181 aactctggac cttcattaat aatagatttc ctaagccgat tacttacaaa atgtttggag
     6241 ctattaaaca tactcttcca aagaaatgga tttattacat tcatgaatac cctgaacaaa
     6301 ctaataaatg tgtattgatt gcagctgtcg aattgttaat tcaatcttta aaacgccatg
     6361 atatttatat tcctcagagt gtcttatata acaatcctat ggattgcttg attgatgaga
     6421 aaacttgggt tcaacaaaaa tacgttttat tagaacaact agatcttcct tcttcagcaa
     6481 atgaaatgtc tttatggtta aagaacgatt tggaactgtc ttataaagaa gcgctaaatc
     6541 gttggcctac ttctaaaatg gccagaatag aaaatgcaga tcgcctggtt gtctctagac
     6601 ttaaaaagac atatgaaaaa aaggaagata agtcttttag acgtcgcatt cagcagttac
     6661 ttcctaaaat tgatttgtca gatttgttat tggaagtaaa ccaacagtta aatcttactc
     6721 aatcatttaa ccacttaagt gacaaggaca ccaaaatgaa agacttggat attagtttat
     6781 tagcagttct tttatcagag tcctgcaata ttggtttttc cccggtttca aaagaaggat
     6841 ttgattcttt aaagtatgat cgtcttgtct atgtcaatca tcattatatt cgagtggata
     6901 cactttcagg agctaatcaa aaaataatac atgcacacag gaaacttgcc acctctaaga
     6961 tatggggtga tgggcatatg gcttcagctg acggtattcg ttatatcaca ccacaacgtt
     7021 ccctatattc tagaagtaat ccaaaatatt ttggccgtgg tcgtgggatc acattctaca
     7081 attttgtctc tgatcaatat atagggttcc acggaatggt cgtttcaggg acactgcgag
     7141 attcgcttta ccttctagaa ggattcctca atcaacccag cgaattaact cctaatcaga
     7201 tcatgactga tacagctggt tatagcgata tgatttttgg tctctttggc ttattagggt
     7261 ttcaatttag tccaagaatt gccaatgata aaggaaccaa actttggcga atcgacgcta
     7321 acgctcacta cggggaactt gatacgttgt ctcaaaattc aattaatatg gaactgattt
     7381 atacccattg ggaggacatt cttagggttg caggttcgtt gaaatctgga aaagtgaatg
     7441 caacagaatt aacgaaatct cttcaacgga atgggcaacc cacgccttta ggaaaagcaa
     7501 taactgagta tggaaaagtt tataagacga aacatcagct tcgttatatt tctgacgaaa
     7561 tatatgcacg gcaaatttta gaacaattga ataaggggga agctcggcat gccttatgcc
     7621 gtaatatttt ttatggacga aaaggaaaac tctaccagac ttattatgat gggatggaag
     7681 agcagctcaa tgcattagga ttagttacta atgccatcat atattggaac tcactgtatt
     7741 tagaaaaagt atttgaacaa atgaaagctg aaggattcga ctgttccgaa gaaatgatac
     7801 gcaggcaatc tccactaatt agtgatcaca tcaattttgt tggtaagtat acgtttagat
     7861 acgaagctga attatccaac ggtaagttac gtccattgaa tgtagaagaa ttctagcaat
     7921 gttcgggttt tgcatgctac cgcactattt cgtatgactg ttccagcgaa tcacttcgaa
     7981 tagaaaaaga gaaaaacacg agaacagcgt taaatcaacg tttcttgtgt ttttactttt
     8041 gtgctaccgc actatttcgt ttctatgttc catgtacccc tttatcaaga agcgcaaaca
     8101 cgccaaattg ccgattggtt gatcggcatg aacgcaagcc ctttatatac cttgaattta
     8161 caacaaaaag gcgtacaagg tacattttca ttaggacgtg tacaaacacc gactttatat
     8221 ctcatttatc aacgccagga agcaatagaa aactttaaaa aagaaccttt tttcctgaat
     8281 aattcatagt tttactaata gtctttccta acattgattt aatagtctt
//
LOCUS       DAXLPI010000110         8175 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXLPI010000110 DAXLPI010000000
VERSION     DAXLPI010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.111"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            171..416
                     /locus_tag="ACF3T5_002218"
     CDS             171..416
                     /locus_tag="ACF3T5_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632778.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(600..1349)
                     /locus_tag="ACF3T5_002219"
     CDS             complement(600..1349)
                     /locus_tag="ACF3T5_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFD3632779.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            1535..2239
                     /locus_tag="ACF3T5_002220"
     CDS             1535..2239
                     /locus_tag="ACF3T5_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFD3632780.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            2279..2836
                     /gene="hxlB"
                     /locus_tag="ACF3T5_002221"
     CDS             2279..2836
                     /gene="hxlB"
                     /locus_tag="ACF3T5_002221"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFD3632781.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            2844..3983
                     /locus_tag="ACF3T5_002222"
     CDS             2844..3983
                     /locus_tag="ACF3T5_002222"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFD3632782.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            3995..4426
                     /locus_tag="ACF3T5_002223"
     CDS             3995..4426
                     /locus_tag="ACF3T5_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632783.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            4440..5885
                     /locus_tag="ACF3T5_002224"
     CDS             4440..5885
                     /locus_tag="ACF3T5_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFD3632784.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            6171..7796
                     /locus_tag="ACF3T5_002225"
     CDS             6171..7796
                     /locus_tag="ACF3T5_002225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFD3632785.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
BASE COUNT         2767 a         1181 c         1635 g         2592 t
ORIGIN      
        1 agaaacaaaa ataaaagtca tgtatatggt ggtcaaaaag taaataatat cagttatcaa
       61 atttcctgta aattttcttg aaactacagc atattattac ttcaaaacaa tctacatttt
      121 ttataaaaaa ggaaatgaat aactgaaaaa attattatag gagagaaaaa atgtcaaaat
      181 ctaaaaaaaa tatcaccata attggtttag gcttaaccgt atatttattt acagaatttg
      241 tttatagaca tgtgaattta tttgcagaac ctttatttgt attatacgaa tcaaattcct
      301 caatcgtcag tgaacttgct aatttaatga tagcattgct gtttactccc gcgttgagag
      361 tcataactat tttggtctgt ttagtgctat tgttttttgt taataaaaag aaataacgta
      421 gtatttcaaa agacaaagaa gtgggatgat gattgctata gtatgttata cgtggtgcta
      481 ggtaagtgag aataaacaaa aaaggaaaca cgcaataaag tttatttcaa aactttattg
      541 cgtgtttctt ttttagattt tttatatctg ttgctttagt agttaccaat agttttcgct
      601 taatcaatca aaacgatttt ttgtttattg tattctttac gaaccttttc aggaatttct
      661 tctgaaataa gataggtaag gtcttcaatt ggaccgacga tatgattact gatggtgccg
      721 aatttatcat tagttatcat cccaagtact tcagtagaaa tacgtatcat ctcacgttta
      781 atgtcggatt cttgcatagt tggcacgctc gttccaacat ctagatctat gttataaata
      841 cccataatat aaaggtcggc tcgaatgttt tgtagttgct tataagcttg taccccaata
      901 ttgatcatag attgtttata aaaagtaccg ccaatattga taatttccaa attaggatgc
      961 tgtgctaatg ctatggcaat cggcgggcta ttggtaatga ttgttgcacg aaaatcttgt
     1021 gggatttctt ttgctaaagc caagttagtt gtacttccat caatgatgat gacagattct
     1081 tcttttaaaa aagggagggc tttatgagct aatgcagttt tttgttcgct agctacagtt
     1141 tctcggtagt taaaattggt aattggcgga ccaacacgta aagcgccgct gtgtactctt
     1201 tttaagagac cttggctatc caaatctttc aaatctcgtc gaatcgtgtc ttcggataca
     1261 ttgaaacgtt gacttaaatc actagcgata actttttgtt ctgcgtttaa taaatctaga
     1321 attttttgat gacgttctaa tttcaacatg ttgcgtgctc cttcttattt attcttgttt
     1381 atcatgtcat ttatgcgttt ttatgtcaat aatttccgtt tttaatacac taaaaaggca
     1441 taaacaagca aaaacaaaat atttttatgc agttttatgt tgactagtat ttgttaagcg
     1501 gttacaatga gggggtaatt aaaggaggga atgtatggag aaaaaaatta ttttgtcgcc
     1561 ttcacttatg tgtggggatc tagcaaattt ggaaggaagc atccaagaac tagaaagtat
     1621 tgggatggat tccttgcata ttgacgtgat tgacggttct tttagtccca gtatgccttt
     1681 agggatcgaa acaatcaaaa gaattagaga aatcacttca atgaattttg atgtacacat
     1741 catggctaag gataacgata acgaattttt tattggagaa atgttgaaaa ttggcgtaga
     1801 gagcattact tttcattatg aaacaagcct ccatattgat cgatatatcc agctcatcaa
     1861 gaattctgga acaaaagtag ggatagcttt gaatccagca actccgttaa atgtattaca
     1921 atatatttta cccgagattg ataggatttg tttgatgctg attaatccag gttttgcaac
     1981 aaacaagtca gaaaaacaag tttcttacgc gctaaaaaaa attacagatc tttgccagtt
     2041 agtaaatgag aaagagttag cagtagatat ccaagtggat ggacgggtat cgttagaaac
     2101 gatcccggga ttattagatg caggcgcaac aaatctggta ttaggaagta caagtttgtt
     2161 tcttaaagga aatacattag cagaaaatca aaaactactt tatcaagcga tgaagcaatc
     2221 agttaggaaa aatcaataga tgtagtggtc ctgaataata gaaatggagg ggaaatagtt
     2281 gagttataag gcggcgaaac aaaatattgt aagagaaatt actgaaacat tagaccgtat
     2341 ttcttcaaaa gatgtggaac aattgataaa gaaaattgaa aaagcggaac gagttttttt
     2401 cgtgggggtt ggcagagtac tgttagcgct agaaggaatg gcaaaacgct tagggcatct
     2461 aggaattcag acagtagtcg taggacaaat cactgaaccg gcaattacag ataaagattt
     2521 attgattgtt ggttctggta gtggggaaag tgctttccca ctaactattg caaaaaaagc
     2581 aaaggaattt gggtcccaag ttgcccatat aggatcaaac ccagacagca caatgaaaga
     2641 atattcagat ctttttattc gtatacccgt acaaacaaaa ttagcgttac cagaggaaat
     2701 ttcttctata cagccaatga ctagcctgtt tgaacaaagt gttcttttgt taggagatag
     2761 tattgcttta gtgatgatag aagaaaaagc aattgatatg tcaaagttgt ggcaatacca
     2821 tgctaactta gagtaggtga aaaatgaaag cggtacattt tggagcaggg aacatcggca
     2881 gagggcttat tggacaaatt ctacatcaga atggattcga tctatgtttt attgatacga
     2941 atcctgaact gatccatcaa ttaaatcaag ataagggtta tactatcgaa attattgatg
     3001 aaatgaacag gagtgatttc attgatcatg ttgtggcact gaatagttta tccgagctag
     3061 aagaggtgat tacagcaaca aaaaatgcag atatcttaac aacatctgtt ggcgcaataa
     3121 atttagtaaa aattgcaccg atcattgcgc aagccttaag taagcgattt gaacaaaagc
     3181 atagcatcaa tatcgtagct aatgaaaatg tgataaatgc aagtgatgtg ttgaaaaaag
     3241 aaatttattc tttgctgtca gaaaaagaac aagagaattt tgatgaatat gcatattttg
     3301 tgaatacggc aatcgatcga caatctttag gaaaagttct tggaggaaaa atgatctctg
     3361 ttgtcgaacc ttattatgaa tggataatgg atcgacagca gttagatcca actacatctt
     3421 ttgatatgaa aaatgtaaat ttggttgatg atatggagcc ttacattgag cgaaagcttt
     3481 atatcgttaa tgctgctcat gcggcagtag cttatttagg cgcactgaaa ggatataaga
     3541 cgattcaaga agcaatccaa aatatggaga ttttcaatct agtcaagcaa tttttaatgg
     3601 agaatataga gtactttgta caaaaatatc atttttctag agaagaatta acatcttttg
     3661 tggaaaaaac attaaaccgt catgggaatc agaaactctc cgatgaaatt actcgtgtag
     3721 gaagttcccc tattcgaaaa ttaggcccta atgatcgttt aattgctccc atagtaaaac
     3781 tagattcata tcatttacct tacgatgcag gaataaaagt aattgctgcg ggatatcatt
     3841 atcggaatct tgctgatcca gaagctgaac aactacgtcg aatgattgta aatgatgggt
     3901 tgaaagcaac gattaaaaag atttctcgtt tggaaggtaa gctgctgaat gaagttgtcg
     3961 ctgcttatga gtcccagatt tagaggagga gaatatgcta aaagaacaat tgtcaggaaa
     4021 tacaatgttt atggatgcga tcgattcatg gcaagaggca atccgagtaa gtgcgcgtcc
     4081 tttgatagga aagggaataa tagaggaaag ctatgtacaa gcgatgatac aaaatgtgtt
     4141 ggataacgga aattatatta tattactgcc actagtagca atgcctcatg cgcgtccaga
     4201 atttgggagc aaaggggtcg ggatgagctt tttatgttta gataagccag tcatgttccc
     4261 aagtgaagaa ccagtaaaat tcttctttac cttttcttct gattcgccaa agggacattt
     4321 agacttaatg gccaatcttg gagaattgct gagtgatgaa aaaatgtatc aaaaattatt
     4381 cgatgtgaag acagaaaaag cattgttaga tttgataaag aagtaaagga gagaagaaaa
     4441 tggaggtagc aaacaaaaaa aaacgagtct ccatgcgagc taaagttcaa gcgattggcg
     4501 gtttcttaac aaatatggta ttgccaaata taggcgcatt tattgcatgg ggaattgtta
     4561 ctgcattatt cattccaact gggtggttcc caaatgaatc tctgaatgaa ctcgtaagtc
     4621 cgacagtcag ctatctattg ccgttactac ttgcctatac tggtggtcgg atggttggtg
     4681 gtacacgtgg agctgtttta ggtacaatcg gaacaatagg tttgattatt ggtgctgaca
     4741 taccaatgtt catgggagca atgattatgg ggccacttgg tgggtggatg atgaaaaaaa
     4801 cagatgattt tttagatggg aaagttcctg ctggttttga aatggtcgtc aataattttt
     4861 ctataggaat tattggtttc atactgatgt gcctttctta tctggtagtt ggaccaataa
     4921 tccaatcagc taatcaattt gtaacgagtg caattgaagc attagtttca actggttttt
     4981 taccgatact gtccttaatc aacgaaccgg caaaagtctt atttttaaat aatgttattg
     5041 accaaggtgt ttattatcca ttaggtatgc aacaaacatt agaggcaggc agatcaattt
     5101 atttcatggt agcatcaaat cctggccctg gcttaggact attgttagct tattctttat
     5161 ttggtaaaaa ggcagcaaaa cagacagcac caggtgcaat catcatccat tttttcggcg
     5221 gaatacatga gatttatttt ccatatgtat taatgaaacc actcactata ttatctatga
     5281 ttgctggtgg aatgacagga atcatcactt ttgaattatt tgatgcgggc ctggtagcag
     5341 gtccaagtcc aggttctatt ttttcttatt tagctctaac gcctcgggga aattttttgg
     5401 gtgtcatggc gggagtggtc acagcaacac tggtttcctt ttttgtaaca tcagtaattt
     5461 taaaagcaga taaatcaaca gatgaagaca gaaattttga actatcagtt caaaaatcga
     5521 aaacgatgaa atcagaagga agaaaattat taaaagaaga aaatgctaga gaaatatgta
     5581 ccgagaaaaa agagatgaat aaaatttcct ttgcttgtga tgcaggaatg ggaagcagcg
     5641 caatgggcgc aacaatattc aagaaaaaat tgcaaaaagc aggtattact gatgttgaga
     5701 taaaaaatta tcgaattgaa gaggtaccag aagatagtga tttggtgatc gtacatcaag
     5761 atttggagga acggactaga aaagcacatc ctaacatgaa aattgtgaca ttaaagaatt
     5821 atttgcaaga tccagtaatc aatggattag cagaagaaat cattggatta aaaacagaag
     5881 actaatttat gggaattttt gattaacctt tattaaaatt attttgccta atataaaaag
     5941 gttgtgacat ggcttgtgtc gcaacctttt tttgtcgcac aaataattaa tcggatattt
     6001 tatgttcata ttttttgtga tcaacgagaa ctgatggggc aaagatacat tttagatact
     6061 tttaactgtt aatttacaaa acaaggtgaa acgaatagaa aaatgaaaaa atcgatacgg
     6121 aagaatatgt ttgattaaaa catgcatgaa gagaaggagg ttgatgatac atgaaaaaaa
     6181 tatattcaaa ggaaatcgat tgttttagag gcattgctgt tttagcagtg atattttatc
     6241 atttgccaaa tagtttgctg ccaggcggat tgcaaggagt aacgatcttt tttgtggttt
     6301 ctggtttttt aatggctaaa aatacgataa gagatgaaaa aaacagacaa ttcagttttt
     6361 tacattttta ctataagcgt atcaaacgta tttatccatt tttactgttt tctttggtcg
     6421 tattcatttt catactaggg attagcaaca ttcgattgat aggaaacatt cgtagtgagc
     6481 tacccagttt gatttttggc tataacaatt ggtggcagct gaatcaagga gtatcttatt
     6541 ttgactctta ctttaatgct tctttattta aacattattg gtcgctttca gttgaactac
     6601 aattctattt gatatggcca tttttgttta ttgtcattaa gcgtatgaga agaaaacaag
     6661 tgttttactt aatctataca ttgatttttg tttctattct ttttagtttg tttttacctt
     6721 caactaaggc atattatcat acgatagcta aactttttcc atttttatta ggagtttggg
     6781 ggtattttaa tcgaataact attggccgtt tctttgaaca aaacactttt agtaaaatct
     6841 ggcttttatt actagctagt ctttgtctga ttctgttccc gatttttcct tatacactaa
     6901 atgaactact gatcagtatt tgttttgctc tattgttagc cagtgtcgat gatatgaaca
     6961 ttgcaaaaaa gaccaaaatc gctagtacaa aactttcttt tgtaggaaaa atcagttatg
     7021 aactttattt ggtgcatttc ccagtattga ttgctctgac aaatctattc aaacatcacc
     7081 attggaaata tttggatatc gcaggtctat tcactgtgtt tttatgtaca ttactcatga
     7141 ttacttgctt tagaaaagga gtgcaaaaga tgagatcacc caaaatttgg acagctatgg
     7201 cactaatgat aatcggagtt tctgtactgg tattacgtgc tcctgccagt cgactgaccg
     7261 ctgaccaaaa tcagttgcaa agtttattaa aagaaaactc tagtaaagca aatgcaggag
     7321 aaaatctgtt gtcacaagag aagaaaatat tatttatcgg tgattctgtt atgctggggg
     7381 cgtatcagga actaaaggag acatttaaaa aagaagcatt ggtagatgca aaagaatcaa
     7441 gacaaatcac tgcgttacca gaaatcttaa aggagcataa agatttagtt tcttaccaac
     7501 aaatagtgat cgggttagga accaatggtg tgataacaga tgaagctata gaacaaacaa
     7561 tgtctttgct gaaagataaa aaagtatact gggtaaatat aaaagcacca actggctggc
     7621 aagatacgat aaatgccaca ttagctacat tgccaaatca gtattctaat ataacgatga
     7681 tcgattggta tagtgagagc aaaaatcatc ctgaattttt ttatgatgat gaaacccatt
     7741 tgaatgaaag tggacgtaca gcttatgcaa agtatattgc ttcagttatt caataaggta
     7801 gtgattacgt gtgttaacct tactttttcc taataggtga atcaatttaa ataaaaaata
     7861 cttcatatga taaacggtgt ttaagaggag gagcttttta atattcgtgg taaaaaagag
     7921 atgggcgagg tcgttaactg agagcttctt ggaaagaaat cgctttggat ttatcctctt
     7981 tgttatacag aacatgagcg agacgacaat actaggatgt atgttctgat cgaagtttta
     8041 tggttacaag cagcaatcag cttagataaa attaaggatc gtaggaacca agtaaagaag
     8101 gctgttagta aagaaacaaa cgatcaagta tgttttactc ttaagaagat gcagtgaata
     8161 aataatagta atata
//
LOCUS       DAXLPI010000111         7955 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXLPI010000111 DAXLPI010000000
VERSION     DAXLPI010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.112"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(13..2184)
                     /gene="copB"
                     /locus_tag="ACF3T5_002226"
     CDS             complement(13..2184)
                     /gene="copB"
                     /locus_tag="ACF3T5_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFD3632786.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2204..4390)
                     /locus_tag="ACF3T5_002227"
     CDS             complement(2204..4390)
                     /locus_tag="ACF3T5_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFD3632787.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="ACF3T5_002228"
     CDS             complement(4390..4599)
                     /gene="copZ"
                     /locus_tag="ACF3T5_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFD3632788.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4612..5052)
                     /locus_tag="ACF3T5_002229"
     CDS             complement(4612..5052)
                     /locus_tag="ACF3T5_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFD3632789.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5126..5665)
                     /locus_tag="ACF3T5_002230"
     CDS             complement(5126..5665)
                     /locus_tag="ACF3T5_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFD3632790.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6280..7746)
                     /locus_tag="ACF3T5_002231"
     CDS             complement(6280..7746)
                     /locus_tag="ACF3T5_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFD3632791.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2162 a         1742 c         1379 g         2672 t
ORIGIN      
        1 cttttttcat tattatttga ttttcaatgt caaagcattt agagctacaa ctaccgtact
       61 taacgacatc aagacagcac caactgctgg acttaatatg ataccaattg gcgcaagaat
      121 tccggctgct agcggaattg caagaatatt gtaacctgcc ccccaccaga ggttctggat
      181 cattttcttt cgcgtttgct tggctagatc aagaaaatgc aaaatatctt ttggatcgct
      241 gtttgtcaat acgacatccg cagaatcgat tgcgatatct gtccctgcac cgattgccat
      301 cccgattgaa gcacgggcta aacttggcgc atcattgatc ccatcaccca ccatgattac
      361 ttttttccct tggtcaaggt atttctggac aacagcttct ttatcatctg gtaataaacc
      421 gccataatat tcttcgatcc ctaaataatc tgctactgct tgggcagcgt ttttattgtc
      481 accagtaagc atgacaggag tgatttttct ttctttcaat gttcggataa attcttttgc
      541 ttctgttttt actacgtcac ctaaagcaac gattgcagct agatgatttt ctaaaatcag
      601 gaagctgatg gtatttcctt gttcttggta aggtttcaat aatgcttgtt ctacgtttaa
      661 tccgagacgc tcaacttctt tttcattaac tattttcact tgttgttttt tgactgttgc
      721 ctctagcccg actcctgaaa gattttttag attttcagct tgataaggct tgatttcatg
      781 ttctttcaaa taattcataa tgccaacagc caatggatga ttggcattcg cttccaaggc
      841 tcctagatat tgaaggattt catttttatt gaacgcatca tcaagtactt cgacacccgt
      901 tacagtaaat tttccttctg ttagtgttcc tgttttgtcc agcatgatca catctagatc
      961 attggcttgc tccagtgcat tgcggttttt caaaagtaat ccgtttttag ctgctattga
     1021 tgtggagcga gcaacaacta atgggatagc taaacctaga gcatgaggac aggcgatgat
     1081 aaagactgtt accatacgtt ctaatgcatt aggaagatct gtcaagaaca accaggcgat
     1141 gaatgccagg atacccacaa ttaatgcgac ataaaacagc cattttgcca ctttgtcaga
     1201 taatgactct agcttggatt tttctccttg tgcttttcgc accatttcca taaccttcgc
     1261 tagataacca ttttcaccag ttccagttac ttcgatttcg atcgttccat ccccattgat
     1321 agaaccgcca atcactttgt cattggcttg ttttttgatt cctcttgatt cgccagtcac
     1381 agctgattcg tcaacaattg tttcaccttt caagattaca ccatctgttg gcattttatc
     1441 accagaccgg acgatcaaac gatcaccttc gtttacttct ttcaatgaaa ctgtctcttc
     1501 ttttccatct ttcgtcaagc gtttcactga ttctggcagt aattctgcta gtttttgtaa
     1561 agcattgctg gcattcgaca cagcattcat ttcaatccag tgacctagta gcataataac
     1621 gatcaaagta gccaattccc agaagaaatc catgacgtgt gtatgtggat tgagcagatt
     1681 tgcgataaac gaatagacac tataaatata tgcaactgta atccccatcg cgatcagtgt
     1741 catcatagca ggactttttt gtttcaattc catcttagct ccacttaaaa atggctgacc
     1801 gccatagata aatagtatag ttgctaagat caatacgagc caatcagaac caggaaacgt
     1861 tacttgaaat ggaaattcca ttcccatcat tggtgaaaaa agaatgattg ggatagctag
     1921 gataagcgat agccaaaatt tttgtttgaa attccccatg tgcatggaat gatccatgcc
     1981 gccgtgttcg tgatccatgt gcatcgaatg gtccatttgt ttgctgtttg ctatatctcc
     2041 atggttatgt cccatgtgca tggaatgatc catctgacta tggtcatgtt tcatctccat
     2101 ctgtttttca tgagattctg tactttcttt gtttactact tcttttttat gctcatgttt
     2161 tttctccata tcgttcccat tcatttttac tccatccctt ctcttacttt ttcatgtgtc
     2221 gatttaagct cagggagttc aacaatacac tgacggaact aaacgccatc gctcctcctg
     2281 caatgattgg attcaaaaag cctagtgcgg caaaaggaat accaatcgta ttatagacaa
     2341 aagcccagaa aagattttgt ttgatttttc tcaatgtcaa cctagacaac tcgattgttt
     2401 gtgcaatgga tgccaaactg ctattcatca gcgtcacatc ggctgtttcc atcgcaatgt
     2461 ccgttccact tcccattgcc ataccgacat cagctaatgc taaggccggt gcatcattga
     2521 ttccatcgcc tgccataccg acttttttgc catctttctg aagtttttct acgtaggctg
     2581 ctttatcctc tggaagtact tcagcaaaga tatgctttgg atcaatacca acttgtttac
     2641 caatcgtttc ggctgccagt ttgttgtctc ctgtcagcat aaaaacatct aatcctttat
     2701 tctggagctg cttgattgct tgtttggctt ctagtttgat ttgatcagcc acggcaatcc
     2761 acccgatcac ttgcttttca tcagctaaaa acatgactgt ttttccctgc tgttctaatt
     2821 ctaacgcgtg ttcttggtat tcatcaaatg ataggttcaa ttcaattaat cgtttacgtg
     2881 tacccgcaaa atagcgtact ccattaatcg taccgctgat cccagcacct gggtgggctg
     2941 taaaatcaat catatcgtat gttgctgctt cttgcagttt tccgtattcc acgattgctt
     3001 tacctagtgg atgttcagaa gcttgttcta aagtataaaa aatagatagg acttcttttg
     3061 aaccgactag atctgtaact ttgggctttc cttctgtaat cgttccagtt ttatccaaaa
     3121 caatgctgtc taagtgagca gctgcttcta atgcttctcc gcctttaatt aaaattccat
     3181 ttcgtgcacc aacacccgtt cctaccataa tagctgttgg ggtcgctaaa cctaatgcac
     3241 atggacacgc aatgactaat acagagacac tatggatcaa tgcgagttgc caatcaccag
     3301 tgatcaaacc tgtagctatg agcgtgataa acgcgatgac taatacgatc ggaacaaaaa
     3361 tccctgagat ccgatctgcg attttttgga ttggggcttt gctcccttga gcatcttcca
     3421 ccatttgaat gatttgcgct aatactgtct gcttgccgac ttgtgtgact tccatgtgta
     3481 gcagaccatt cgtattgatt gtcccgccaa aaagcgtgtc atccggattc ttttctacag
     3541 gaagattttc accagtaagc atactttcat ctatcgcact gcttcctgaa ataattcgac
     3601 catctgctgg aatctgctca ccaggacgga tgactagttg atcaccaacg actacttctt
     3661 caatcgctaa cgtttcttct ttcccgtttc ttatcacttg ggcggttttt gtttgaagag
     3721 acatcaattg tttgattgca ttacctgttt tcgtttttgc tgtatgttcc aaatatttgc
     3781 cgagcaaaat cagcgtgatg atcatactgc tgctttcaaa ataaagatct tgggggtgtc
     3841 cacggaaaaa tccgttgtaa atgctcaatg caaaggcagc cgatgttcca attgctacca
     3901 atacatccat atttggtgct ttcgtcttga ttgcatggta cgcaccttta taaaaacgag
     3961 caccgacata aaattggaca ggagcagaca gcactaactg gacgattgga aaatgaaaaa
     4021 agtgaacaat cgccgcatgg cttcctaaca tcatagcaat cattgacaac atcaacggca
     4081 aagtcaaaac cgtactcaat ataagatcga atagcatttt tttcaaatac gcttgctttt
     4141 cttctgcaat tttttgtttg tgggcttcat cgtacaaaat tgcaccataa ccgatgtttt
     4201 ctacgctttg gattaatttt tcagtggtcg tcccttcata tttgacactt gctttttcag
     4261 tggccaagtt gactgtggca ttgaccacac caggttgtcc gttcagctct ttttcgatcc
     4321 tagcagaaca atttgcacaa gtcatacctg taatgacgaa cgtatccatt ttggtttctg
     4381 ctgttgccat caaatcacct ctgctttata acccaatcca ttgattgctt gacaaatctt
     4441 ttcggattga acttttgttt catcaaactt gaccgtaccg tttgcttttt tcaaatttac
     4501 tttcactttt tgcacgccgt caagtgcgct tactgtttct tctactcttg ctgcacaatg
     4561 attgcagctc ataccttcga ttgaaaattg ttgtttcatt ttttcttcct cctattgatg
     4621 ttgtttacat tcacattgac ctgggataca gttacattcg atcgtatcta ctggttgctt
     4681 ttgttccagt tgtttcaaaa tctcattgat atcttcttga gtcaaagtgg cttcttctac
     4741 tagatccgca atcgtctctc caatcctttt tgcacagata tgggaaaaaa gattttccgt
     4801 cgcactgcga actgtttcca tctccgagac agctggatga taaatgaatt tttttccttc
     4861 ttgttctgtc cagatcactt ctttttttac caaacgtccc agcaaagttt tgatcgttgc
     4921 tggtttccaa tccatcgaat cagctaagat ctgcgtgatt tcttgggctg tcgtctgtcc
     4981 aagtgtccaa ataacacgca tgatctccca ctctgaatca ctgatcttta ctggcgtttt
     5041 gactttttcc atttatttca cacctttcgt ttacaaatgt aatcattagc ttatgaatac
     5101 agattacgct tgtaaacaaa aactgtcaat tatttttgct ctctattttc aatttagtta
     5161 aaaattcttg tttctctcgt cttgttttaa ggatgatcag tttatttctt gcattagcta
     5221 cttggatcag ctgctgtatt ttcggttcat tgttttcttc aaaatgccag ataaaagata
     5281 aaaattctaa gtattccctg tttagtttct ctgaaaatga ttcaggcata tctggccgag
     5341 attttttgtt tatctttctt tttagagaac gccaaactac tcggtagatg gcgagataac
     5401 gcggcacttt aagccaaatg atcttatctg cttctttaat cctttcctct aatgtagaag
     5461 tataatttcc ttcaacaatc catgacggat tagataccat aaacgattgc tgttcttgca
     5521 aaaaccactt ttttgcctga atactgtagt ctgttgtatg ccataaaagg tctagcggca
     5581 gtagcggaaa gtcggtgatt tctttgatct tccttgcgag ggtcgatttt cccgacccac
     5641 tttgtccaat cacgatgatc ttcattcttc tccctcactt ctctttggaa tttggttcga
     5701 tcataccact tataacgccg caaaaaaaca agggactgtg acaaaagtcg tgtcaaagtc
     5761 ccttgtttcg aataaatggt gttcaatcag ctgaccttca gcattaactc aaaaaacggc
     5821 aaaatatgag gagctatttt ttcgtttgtt cctctaatac tcaggtcaaa tcgctgattt
     5881 tacaacctct tccttaaacg gtgttctcct gccaacacct cggtcacaag gcacaatagt
     5941 caacaaacat gagaagttgt tttttgaaat tgctccttgt gactcggagt taaacggcag
     6001 gctcactacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
     6061 aaaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
     6121 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttctgcgaa aaacaagccg
     6181 aaacttcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
     6241 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
     6301 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
     6361 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
     6421 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
     6481 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
     6541 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
     6601 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
     6661 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
     6721 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
     6781 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
     6841 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
     6901 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag gaatattatc
     6961 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
     7021 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
     7081 aggggatatt ttttccgctc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
     7141 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
     7201 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
     7261 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
     7321 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
     7381 cgtatttacg atatttccat ttccttggac tgcccaaccg ccagcaatca ggattgcttg
     7441 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
     7501 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
     7561 gaaggctgac cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
     7621 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
     7681 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
     7741 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
     7801 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aatacacatc
     7861 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc atttacctta
     7921 ttgaatttca agctattttg aattttctgc aaaat
//
LOCUS       DAXLPI010000112         7798 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXLPI010000112 DAXLPI010000000
VERSION     DAXLPI010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.113"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(590..1936)
                     /gene="gor"
                     /locus_tag="ACF3T5_002232"
     CDS             complement(590..1936)
                     /gene="gor"
                     /locus_tag="ACF3T5_002232"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFD3632792.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2070..3323)
                     /locus_tag="ACF3T5_002233"
     CDS             complement(2070..3323)
                     /locus_tag="ACF3T5_002233"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFD3632793.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3329..4252)
                     /locus_tag="ACF3T5_002234"
     CDS             complement(3329..4252)
                     /locus_tag="ACF3T5_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFD3632794.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4473..6965)
                     /locus_tag="ACF3T5_002235"
     CDS             complement(4473..6965)
                     /locus_tag="ACF3T5_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFD3632795.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6972..7439)
                     /locus_tag="ACF3T5_002236"
     CDS             complement(6972..7439)
                     /locus_tag="ACF3T5_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFD3632796.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7575..7648)
                     /locus_tag="ACF3T5_002237"
     tRNA            complement(7575..7648)
                     /locus_tag="ACF3T5_002237"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7612..7614),aa:Arg,seq:acg)
     gene            complement(7706..7779)
                     /locus_tag="ACF3T5_002238"
     tRNA            complement(7706..7779)
                     /locus_tag="ACF3T5_002238"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7743..7745),aa:Pro,seq:tgg)
BASE COUNT         2173 a         1697 c         1255 g         2673 t
ORIGIN      
        1 agaaactggc ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt
       61 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
      121 aaggacagct gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt
      181 ttcacagtcc cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct
      241 tggtttggta actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta
      301 tacaaagtca cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat
      361 cgataccaat attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag
      421 ttacgaagat aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag
      481 caatcttaca taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg
      541 ttttacatgg tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg
      601 ttacgaattc ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag
      661 ttgcacccat cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa
      721 tagcatgtaa gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt
      781 tttggcgata atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca
      841 attcattttc gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg
      901 gtggatgcgt aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc
      961 cattaaataa acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta
     1021 atttaccgat cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat
     1081 ctactttgat aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt
     1141 cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat
     1201 tttcaaatag caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg
     1261 cgtaagtttt tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat
     1321 caaacgttct aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac
     1381 aaaatacacc tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag
     1441 gtaactctgt taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg
     1501 tcattttact tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac
     1561 cgtttacttc gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat
     1621 gattgctatc taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc
     1681 tttctactag accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg
     1741 catctcgttc aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac
     1801 aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc
     1861 catgcatccc tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt
     1921 aatcatatat ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa
     1981 cgatcctttt tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg
     2041 cttaaaacag ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt
     2101 ttcaccggct gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg
     2161 gattcgcctt cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga
     2221 ccataaaatt cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc
     2281 tgtgttgcct catcataaga taatacttca ccgacaaatt tatatactgg gattttacga
     2341 cgttcaccga ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat
     2401 tctcgttgtg ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca
     2461 ggatcttcca tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa
     2521 tggatcgatt tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct
     2581 ggaatatgtt cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg
     2641 cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc
     2701 cagcgacaag attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac
     2761 aatgtgcatc gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg
     2821 gtatccgtat ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg
     2881 acacgttcca acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct
     2941 tgagtggata aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct
     3001 ggatcagaaa caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct
     3061 gctccatctt catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca
     3121 tgttcttttg caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg
     3181 cttcttaaac catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg
     3241 gtttttaact tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt
     3301 tttactgtca tttctttcat catactcctt atttgatagc agaaggatca atataaaaga
     3361 atcctgtatc taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt
     3421 ccgctttttc ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc
     3481 gagtgatgtt tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt
     3541 aatgatgctg ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa
     3601 tatgtgtgcc atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg
     3661 agataaaaag acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat
     3721 tctgtagcag tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta
     3781 caggaatatc gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa
     3841 gtactgctcc aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta
     3901 atgtttcagc atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa
     3961 taatacctgt atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat
     4021 attcaggtac atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt
     4081 ttttaccata cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag
     4141 caggcagacg caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa
     4201 gcaactgttc tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct
     4261 cctcctacaa cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt
     4321 aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt
     4381 ccaaatataa aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact
     4441 attataaaaa ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta
     4501 ggtccgcgaa cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct
     4561 aaacgaattt ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt
     4621 gcgcgacgga ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg
     4681 attgcagatg gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct
     4741 ttgctcatga tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca
     4801 gtctcatcga ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata
     4861 cgtttttgca tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt
     4921 tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag
     4981 tccacttttc tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa
     5041 ttgaaaacgt ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt
     5101 tgacgaattt tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac
     5161 ccgattaatc ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg
     5221 gcatcttcac ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca
     5281 ccagttggtc caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt
     5341 cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt
     5401 tcatgcagaa gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt
     5461 agagggacac cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca
     5521 gaataacctt gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt
     5581 gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct
     5641 tccttttctt gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga
     5701 tcggtttgat ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg
     5761 ggtagttgac gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca
     5821 tctgtgatct ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact
     5881 tctgcttctt caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca
     5941 gaatcttttt cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat
     6001 tctcctcgag ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca
     6061 cctgcaccaa ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag
     6121 atttcgtcga tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca
     6181 accaaagatc ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca
     6241 cctaaaatga ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca
     6301 cccaccagta cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact
     6361 tctctcgaac gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca
     6421 cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt
     6481 acatttctac gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt
     6541 ttcagaactt gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc
     6601 atgatacttt catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa
     6661 ccagttttct tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat
     6721 ggaagaaaga ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt
     6781 tcttcataaa tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca
     6841 ttttgctcga ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac
     6901 cgttttgctt cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct
     6961 tccatctaat ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc
     7021 cgagcacgtg tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc
     7081 aacatcaagt ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata
     7141 gcaaaagcat ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat
     7201 tgttttttat ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg
     7261 ctttctacta gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt
     7321 gaaggaacac agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta
     7381 ttttccagaa ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc
     7441 ttattcattc cctttgacta atattgacct taattataac gaatttgata atagaaagaa
     7501 agaagaacgc tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata
     7561 ctattccgta tcgacgcgcc caagaggagt cgaaccccta accttttgat ccgtagtcaa
     7621 acactctatc cagttgagct atgggcgcat attcaattta aaaaaattat aaatcatttg
     7681 aaaacaaaaa gcaagataaa aaaaatcggg aagacaggat tcgaacctgc gaccccttgg
     7741 tcccaaacca agtgctctac caagctgagc tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DAXLPI010000113         7626 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXLPI010000113 DAXLPI010000000
VERSION     DAXLPI010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.114"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(15..713)
                     /locus_tag="ACF3T5_002239"
                     /pseudo
     CDS             complement(15..713)
                     /locus_tag="ACF3T5_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            792..968
                     /locus_tag="ACF3T5_002240"
                     /pseudo
     CDS             792..968
                     /locus_tag="ACF3T5_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="ACF3T5_002241"
     CDS             complement(1144..1713)
                     /locus_tag="ACF3T5_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFD3632797.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="ACF3T5_002242"
     CDS             complement(1787..2110)
                     /locus_tag="ACF3T5_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632798.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="ACF3T5_002243"
     CDS             complement(2264..3076)
                     /locus_tag="ACF3T5_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632799.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3403..4278
                     /locus_tag="ACF3T5_002244"
     CDS             3403..4278
                     /locus_tag="ACF3T5_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3632800.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4292..4615
                     /locus_tag="ACF3T5_002245"
     CDS             4292..4615
                     /locus_tag="ACF3T5_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632801.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4612..5364
                     /locus_tag="ACF3T5_002246"
     CDS             4612..5364
                     /locus_tag="ACF3T5_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFD3632802.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="ACF3T5_002247"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="ACF3T5_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2272 a         1512 c         1372 g         2470 t
ORIGIN      
        1 agggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacctatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caacgtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtgtgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg taggatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcggcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttc gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 acttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gttacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgagaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatga atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagctttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaacg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctcgaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caacct
//
LOCUS       DAXLPI010000114         7422 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXLPI010000114 DAXLPI010000000
VERSION     DAXLPI010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.115"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..250)
                     /locus_tag="ACF3T5_002248"
     CDS             complement(<1..250)
                     /locus_tag="ACF3T5_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632803.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISL"
     gene            complement(368..628)
                     /locus_tag="ACF3T5_002249"
     CDS             complement(368..628)
                     /locus_tag="ACF3T5_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632804.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(765..1178)
                     /locus_tag="ACF3T5_002250"
     CDS             complement(765..1178)
                     /locus_tag="ACF3T5_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFD3632805.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1634..2119)
                     /locus_tag="ACF3T5_002251"
     CDS             complement(1634..2119)
                     /locus_tag="ACF3T5_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632806.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2258..2473)
                     /locus_tag="ACF3T5_002252"
     CDS             complement(2258..2473)
                     /locus_tag="ACF3T5_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFD3632807.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2697..3497
                     /locus_tag="ACF3T5_002253"
     CDS             2697..3497
                     /locus_tag="ACF3T5_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD3632808.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3516..5384
                     /locus_tag="ACF3T5_002254"
     CDS             3516..5384
                     /locus_tag="ACF3T5_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFD3632809.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5377..5868
                     /locus_tag="ACF3T5_002255"
     CDS             5377..5868
                     /locus_tag="ACF3T5_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFD3632810.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5856..6410
                     /locus_tag="ACF3T5_002256"
     CDS             5856..6410
                     /locus_tag="ACF3T5_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFD3632811.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6428..>7422
                     /locus_tag="ACF3T5_002257"
     CDS             6428..>7422
                     /locus_tag="ACF3T5_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFD3632812.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2633 a         1193 c         1418 g         2178 t
ORIGIN      
        1 taagagagat cataagcccc aatgaatgac taaaaagaaa agcagaatca catgccatat
       61 catcccatat ttcttcaaga aaataaataa gcgggaaaaa gggaaggaat agtagaacat
      121 cgatccagca ccattttttt cttcttttga aataacataa aacaataaac atgaacgtgc
      181 agggaagtaa aaacaaagct ttactcataa ctaacaaaat accagctcct tttatttttt
      241 tctcccccac attcaaagag taacaatggt atgtacttaa taatagaaaa atggatcaac
      301 ttgtatgaat aacagctgaa aagatctgca ccaaataaaa aacttccagc caaaactgac
      361 tggaagttta tttttttctg aaatattcta acacaaatat gaaaaagatg gccgaagcta
      421 ataagccgat ttcgtagcaa aacaatgaga taccagagtc gtaataataa aatcctctaa
      481 tcttatggat aaaatagatc atcggacaag cagataagag taagtatttg ttaggccttt
      541 tgaataagta aaagaaagca ctggtcatta tgataaatgg tatccaaaac aaaagtttac
      601 ttaagacaat caaatgatca actcccatat tttttaccaa gagagatcat aacatcttta
      661 tcgtaaaaac aaaatatttt tttgctcatt ttgaaaattt aaacatactc gttcttatcc
      721 aaatatataa aaatcgcccc tcattcatcg agaggcgata agttctaatt taactcaatt
      781 ttttttgttt cttttggctg ataagctgtt ttaggtaata ccaatttcag cacaccgttt
      841 ttaaaggttc cagtgatttt ttcttcatca acattaggaa gatagaatga acgtttgtaa
      901 gagaccgaat gtctttcttt ccgtacatag ttgccatctt ctttatcttc tgtatgggac
      961 tcatgattgg cggagatcgt caaagtatta tctgaatatt caactgtcgt gtcttctttg
     1021 tcacagccag gcatatcagc agttagtttg tattcttttt cattttcgac taaatctact
     1081 tttggataac ttgaaacacc taaaaaatca ttgaatgctg gactaaacac gtcattgaaa
     1141 tcagggaaca tgtcgcgaat gctaggtaca tttgccattt ttgatcatcc tttcaataag
     1201 tattttttat ttatccctat ttacgtaata actctatttt gaaaaaaatg caagtataat
     1261 ggttagcttt gtgaatattt catctaatac gatacaaagg ctatctttct aatttgaaaa
     1321 agggatcatt aaaataataa gaagaaaata tgtcaaaagg taaaaaagta aattcttcta
     1381 tccaaatata gaaaaattga atctagaaaa ccgcccgaca ataaatttca ttaactcagt
     1441 tgaccgaaaa tgaagtagat accaaaaatt tatcaagaga gcaagtagag aagtgagtat
     1501 attcataaga gaaatagctg taacgaatta ttaggttaat gcaaatgctt atttatactc
     1561 cgtataactt tctcagaaaa aatgatacca ataatgactt gctagtactg taaatccgta
     1621 taatatcatc taatcaaaat ggtgttccat ttgcatctgt catcgtaatc gatccttcgg
     1681 gtgacactcg ataaaaacca acgcttccag atcctccttg ggaacggatt tgtttgctgg
     1741 aagctgtgaa caaataatct cgatcaattt tttgataaaa gctaataaca atatcgtcac
     1801 tttgtaattc ggaacggtgg gcaataagaa atagaagtgc tttgtcagag ttttctaatg
     1861 gaagagaagg tacagtgctt tcttgcattt cagatgttga agaaatgcta aacgactgaa
     1921 tacttgattg gctatgttgc gaatctacat taccagtaga agtattagta atggaagtag
     1981 tagaagtact tttagttgaa ccaacctcag ttgttgaaat ggctgctgaa gattctttaa
     2041 cagaattctt tgttttatat ccacaggcag aaaaaagtat tacagttccc aacaagcctg
     2101 cgtaaatccc tttatgcatg atagctcctc ctaaaataga ttatctttat tcagtatatc
     2161 acttaaacaa agaaaagcag atggtttaaa atgaaaaaac cttcccaata aagggaaggt
     2221 tcatgaagat aataactttt ttaataatta tagctttcta atatctttca tcaacttttc
     2281 tagtaatcat tgggaaaaat ttcaagcagt agatcgcaca aatacctacg acgatataaa
     2341 tgatcttcgc aaaaatagtt gttgaaccac cagcaatcat agctactaaa tcaagttcga
     2401 acaagcctac caacagccag ttcaaccctc ccacaatcaa taaagttaat gcaatagcat
     2461 ctaaagtttt cattagaatt ctcctttctt aaatgataaa acaattatat atcaaaaaga
     2521 gaaacattgc ttatataacg ctcaggaatt ttagcaatct atgcaaaaag atgaaaattt
     2581 tttttgctct tccattagta cacatttttt agctgaagct gtaaatttca tgtcttaatt
     2641 ttttttagtg agcttttata ctggatttgt aagaaaaaag aaggagagga tcacatatgt
     2701 cggattggtt ggatattgca ggaaaagtag taattgtaac tggaggttcc tcaggaattg
     2761 gaagaagtat cgtcgaaaat ttgttgaagc agaatgcaca agtagcgaat tttgatgtaa
     2821 ctgaatgccg tatacagcat gaaaacctac tatctttaaa agtcgatgtg tcttctaaaa
     2881 cggacataga agaagggata tataaagtaa tgaaacattt caagacgatc gatggtctag
     2941 tcaataatgc aggaataaat attccttctc ttctaataga tagaaatcat ccaaaaagca
     3001 agtatgaatt atcagaacaa gtttttgata agatgatcgc tgtcaatcaa aaaagtgtat
     3061 atttgatgag ccaagcagtt ggaagaatct tggtgcaaaa gggatcagga gtgatcgtga
     3121 atctttcttc tgaatcagga ttggaaggat cagaaggaca gagctgttat gctgctacaa
     3181 aagcagcgat gaacagtttt actcgttctt gggcaaaaga gttaggaaag aggaatgttc
     3241 gtgtggtagg tgttgcacca gggatactgg aagaaaccgg tttgagaaca caagaatatg
     3301 aagaagctct ttcttatacg agaggtataa gcgtcgaaca gctgagaaac ggttacagtc
     3361 gaacaagtac gatccctcta ggaaggagtg gtaagctgca agaagtggcg gatctagtct
     3421 gctattatct ttcagaaaga tcaagttata tcactggagt cacgactaat atttcaggag
     3481 gtaaaacaag aggataaagg atagggggga cgcacgtgac gctagtaaac agatggtacc
     3541 aattattgaa attactagtc actcacaaga aagtttcaat ccaggaagtg caaaaagaaa
     3601 tcaaaaccag taatcaaacc ataaaaaaag atattgggga gttgaatgaa gaaattgccg
     3661 gtattgcaaa aatcatccaa aaaaacaaac attttgaact tgaaatttat gattttgatt
     3721 tttttgatga gatcatgcaa ggaaaattga aaacagccag tgatttcaat tcggcatcca
     3781 aaagaatcgc ttatttacta aataggctga tcgaaacaga ggatttctta cgtatggatg
     3841 atttgtctga agaagtcggt gtcagcagag gaacagtggt caaggatcta aagacattaa
     3901 aagagatgat caatgatttt gatgtaaaga ttacaggcac accaaataaa ggactaagaa
     3961 taataggaga tgagctagaa ttacgactac ttttatttta ttttgtttct ccctactttt
     4021 cagagacgaa aacagaacag tttctagaag aaaaacaagc aatcaaacat tttcagaaga
     4081 agacgaatgc tagtaaacaa gaaatcaccc ttttaacgaa agttatcggt atttctttga
     4141 atagaatctc tagtaactat ctgttgaaga agcccatcaa tcgttacagt gggtgttttg
     4201 aatatatcca agaattcaat gaactgatca atcagttgga agaaatctat gaactaactt
     4261 tgagccaata tgaaaaagat tttatcagtt ttcctttgaa catcatcaac acaagtataa
     4321 cgatgataag gaaagctaac tctgatttgc gcttatattt cgaccccatg atgatgcgta
     4381 ttcgacgatt agatctcatt gatctggatg agtccttctt atatcaagag atgaaacagc
     4441 acctctcttt attgataaat cgggtaattt ttcgttatcg attgactgat ttattttatg
     4501 gtgagataga aaaaaagtat cctttttctt accaaatcgc tagcgaatgc atacaagcat
     4561 tagaagctat cttgcaacat aagatctcta aagtagagat cagctaccta acgttgtatt
     4621 tggagttagc atttcgatat caaaaagagc atttttcctc taaaaaagta gccattgttc
     4681 atacgacagg aaaaggaaca gcattaatga tccaaagaca aatcaaaagg atattgggga
     4741 gggatataca aataacacat gtttcagaag aacaatatga aaaagtagat ctaaacaaat
     4801 atttcgctgt ttttacaacg attccgttaa aaaatgtaaa tgaagagaca ccggtgatcc
     4861 atttgactaa tctttttgat gactcttggt taagggcaca atggcaaaaa gcaaaaaaaa
     4921 agcgattagc cgggtttcat tctactgttt ttttctatca aatatggaaa gaaaaaaaga
     4981 cgtatcaaga atatttaaac aaattagtcg ctcctttgat cagaggctat tatgtggatg
     5041 aagaatttga aaaaaggatc ttgaaaagag aatatcagca aagcacattt gttaacaaag
     5101 aatttgcttt tcctcacaca accaaccagc tatctcaaca cattgtttta tcagtagggc
     5161 taataactga tcaaggttta gcagaaacag gaggaaatgg agttattttt ttatttgcca
     5221 tacctgaaga aatgacggct gaagttgaga atgaattatt tgagctatac gatatcctat
     5281 ttgaactcat aaaaatggga atagatgagt ttattgaatt agcaccgaca caagaatcat
     5341 ttttaaaact aatcaaaagt aaaggagaga gacttcatga atgatatgta tctattcata
     5401 gttttcgcag cttcagctta tgtgctgcaa gcagttatca gttggaagca actaaagcat
     5461 ttcaatcaaa catatagcga actgcgaaga aaaggaaaag tagcgattgg taaacgacct
     5521 gggaaaatcc gttcaggcac gattgttatg ctagctatag acgatcatga tcaagtttta
     5581 gaagtcagaa aaatgcaagg ggttacatct ttggcaaaat ttcattcatt acctgtctat
     5641 gcgggtgaag atatccatta catcgatcgt taccatccct cagttcgagc ggaagatcgt
     5701 ttgacacaag cagctttaga aaatgccaga gaagtatact tacgaataga ggcaggaaat
     5761 gaaaaaagtg aacaaccttc attgcctttt attgctatgc tcaataattt tcggatatgg
     5821 aaaagtacga tcaaagaaaa aaggagtgac aaacgatgga tatcataaca aaatttgctg
     5881 agggatttat gaggctgttc caaacaggtg cagaaacatt cataagctgg atgacaggaa
     5941 tcgtaccagt tgtcttgatg ctgatggttg caatgaatac gctgattgct tttttaggtg
     6001 aagaccgagt aacgaaagta gccaaattat cagcgaaaaa tccgcttact cgttaccttg
     6061 tgttaccgtt cttatctgct tttatgttgg gaaatcctat gtcatttaca atggctcgtt
     6121 tccttccaga atactacaaa ccaagctatt atgctgctca agctcaattt tgtcatacga
     6181 gcaatggtgt attcccccat attaatcctg gagaattgtt tgtatggatg gggattgctc
     6241 aagggatcga aaccttaggg ctaaattcga tggagctggc tatccgatac ttgcttgtgg
     6301 gactgctgat gaactttatt ggtggttgga taactgattt tactactgga tttgtatgca
     6361 gacagcaagg aatcgttctt agcaaaaaag tagagctttc agttgattag agaaaggaga
     6421 gttttaaatg tatggtgata ttaagatcgt aagaggaaac ggcgggtatg gaggtccttt
     6481 gattattagg ccgacaaaag ataaacacaa gttcatctat gtcacaggtg gaggtgagag
     6541 gccaatgatc gtagataaaa tagaagaact gacaggtatg gagtctatca atgggttcaa
     6601 aacatcaata cctgatagtg aaatcgcgct tgcaatcgtt gactgtggag ggactcttag
     6661 atgtggaatt tatcctaaaa aagggatacc aaccatcaat atcgtttcca caggaaaaag
     6721 tggcccatta gcacaatata tcactgaaga tatttatgtt tcagcagtag ggttagaaca
     6781 aatacagcca ctaatggaaa ctgatgataa caaagaaaca gtaaatactg catctccaga
     6841 taaaaagtac aaatatgata cttctaaaaa gataaccgag caaaaagcag agcagcaaaa
     6901 ctggatagca aaaattggaa tgggcgcggg aaaagtagtt gctacattca atcaatcagc
     6961 aagagaagca gtagatacga tgatccggac agtgatccct tttatggctt tcgtttccct
     7021 tttgattggt gtgatacaag gttctgggat cggtaatgta tttgctaaca ttatggcacc
     7081 tttagcagga aatgcagttg gattaacaat cattggcttt atttgttcac tgccattttt
     7141 atctcctttg cttggtccag gggcagttat tagccaagtg attggaacat tgatcggtgt
     7201 agagattggg aaaggaaata ttcctcccca attagcatta ccggcactat ttgctatcaa
     7261 tactcaaaat gcatgtgatt ttattccagt cggtctaggt ttggaagaag caaaagctga
     7321 gacagtagaa gtaggtgtac catcagttct ttattcaaga tttcttaacg gcatcccacg
     7381 agttatcatt gcttgggtag caagttttgg attatatagc tg
//
LOCUS       DAXLPI010000115         7106 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXLPI010000115 DAXLPI010000000
VERSION     DAXLPI010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.117"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(14..2248)
                     /locus_tag="ACF3T5_002258"
     CDS             complement(14..2248)
                     /locus_tag="ACF3T5_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3632813.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            2727..2993
                     /locus_tag="ACF3T5_002259"
     CDS             2727..2993
                     /locus_tag="ACF3T5_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFD3632814.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            2993..4720
                     /gene="ptsP"
                     /locus_tag="ACF3T5_002260"
     CDS             2993..4720
                     /gene="ptsP"
                     /locus_tag="ACF3T5_002260"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFD3632815.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            4884..5930
                     /locus_tag="ACF3T5_002261"
     CDS             4884..5930
                     /locus_tag="ACF3T5_002261"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFD3632816.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            5947..>7106
                     /locus_tag="ACF3T5_002262"
     CDS             5947..>7106
                     /locus_tag="ACF3T5_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFD3632817.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMR"
BASE COUNT         2241 a         1296 c         1459 g         2110 t
ORIGIN      
        1 tataaaactg gatttattca gccgtttctt cggctgattc tttagccaaa cgttctggtt
       61 tagaagtaac gatgattttt ccatctttat ccagttttgc tttcaactca tggatagaag
      121 ggtgatcaag ataaaattca gcgataccat cttcgatttg ttcttggatc gtgcgtcgaa
      181 gaggacgagc acccatagat ggatcatagc ctaagtcaac taatttttct ttgacattcg
      241 taggaacttc gatgtgaagt tgctgtgctg ccagcatttg gttgacatcg tctagcatta
      301 gtgtgacgat tgtcatcaag ttttcttttg ataatgcact gaattcgatg atcccgtcaa
      361 aacggttcag aaattctggt gtaaagtagt tgtttaattg gttcaataca gaacgagtaa
      421 caccttcgcg tgccgcacca aaaccaacgt ttgcttctac tttaccagtt ccggcattac
      481 ttgtcataat gatgatcgta tctttaaagc tgaccgttct tccttgtgca tctgtcaatc
      541 gaccatcatc taagatttgc aagaacatat gcaaaacatc tggatgagct ttttctactt
      601 catctaacaa gacaagactg taaggatttc tccgtacttt ttctgttaat tgaccagctt
      661 cttcgtatcc gacataacct ggaggagaac caatcaattt agagacacta tgtttttcca
      721 tatattcaga catatcgaaa cgaatcattg aatcttgtga accgaatagt tcgtaggcaa
      781 gttgtttcgc aagctctgtt ttacctacac cagttggtcc aacaaataag aaggagccga
      841 ttggacgatt ctgtttgttt aatccaacac ggtttcggcg aatcgctttc gctactcgat
      901 ctacagcgtt atcttgtccg atgacatgag ctttcaagtc atctgctaag tttttcagtt
      961 gtgtctgttc tttttctttt aattcaccca ctggaatacc tgtccgttgt tcaacgattt
     1021 tttccatatc tttttcggta atcacaggtg tttcctcttc agtgagctga cgttctttca
     1081 ttttttgaag tttattgatc tgatcgcgat aataagctgc tttttcgaaa tcttcttctc
     1141 gagaagctaa aactttttgt tcttctgctt cttgcagttt tttctcgatt gttttaggat
     1201 caacgatttg gatcgtcaag tttttcttag aacctgtttc atctagcaaa tcgatggctt
     1261 tatccggcaa gaagcgatct tggacgtaac gattggataa agttgcggct gcttcgatgg
     1321 cctcatcagt atattttaca tgatgatagt cttcataacg tttttgcaaa cctttcaaaa
     1381 tactgatcgt ttcttcgaca gttggttcat ctacccgtac aggttgcata cgacgttcta
     1441 aagcagcgtc tttttcgatg atgcggtact catttaaggt agtagcacca accatttgca
     1501 attctcctcg agctaaagct ggttttaaaa tattaccagc atccatattt ccatcaccag
     1561 cagaaccagc accgacgatt tcgtgaactt catctatgaa taggatcaca ttttctgctt
     1621 gacggatctc ttcaatcaat ttttgcatac gttcttcaaa ttgtcctcgt ataccagtac
     1681 cttggactaa agatacgaca tctaaacgaa tgacttcttt gtccattaat ttttgcggta
     1741 catcaccatc tacgattttt tgtgctaaac cttcaacaac tgctgtttta ccaacacctg
     1801 gttctccaat caagacagga ttgttcttgg ttcggcgatt taaaatttca atgactcgtt
     1861 tgatttcgtc atcgcgtccg atcacaggat cgatatcacc ttgtcgtgct tgttctgtga
     1921 tattgattcc gtattctccc aataaacctt ctgtattgcc ttgaggtggc tgaccaccat
     1981 tgaagttgtt gtttccgcca aattgtgttg gtggtgtttg ctcataattt ccttgctgtt
     2041 gcatttggcg agacatggaa cggaaaagat cgtccaaact gccaaatcca aatggatcat
     2101 tttgggccat attagtcaga cctccatttt gttgattttt gattttttga taacagctct
     2161 gacagtaatc gagttgtgtc cgctgaccat tgacagtggc atacaagtga atcgttgcct
     2221 cattcttgcc gcagttttga caaagcatga atcattcacg tcctttcaca aaaatgggta
     2281 catccatatt ttacatctcg tttttcatga ggtaaaagaa taacccctag ttttttaaga
     2341 gatttatgag gtagaaaagg tgaaatctct attgaccatt cctgaccttt gactcttatt
     2401 atactaaaaa taaaaaggga agcaagtaat tcgtttcata tgaaaacaaa gagggtcaaa
     2461 ggaaagaatg ataaacgtca ggagaagaga aagcgacaag aaaaaggaaa taaacacgtt
     2521 tgttataaaa tgtgaagaaa aggatgaaaa agaatccgga ataatttgaa tacttttatc
     2581 tgaaattgaa tgaaaactgt tgtatctaac gaaaaaaaat gttaatcttt acagatgaaa
     2641 gggtggcatt tgttaggatt ctctgaatta ctaaaaaaga aaaccctcac ataaaaacaa
     2701 cactcactta aaggagaccg attaatatgg aaaagaaaga atttcacgta gtagcagaaa
     2761 ctgggatcca cgctcgtcca gctacactat tagtacaaac agcaagcaaa tttaactctg
     2821 atgtaaactt agagtataaa ggtaaatcag taaacttgaa atcaatcatg ggcgttatgt
     2881 ctttaggtgt aggtcaaggt tctgatgtta ctattactgc agaaggtgct gacgaagctg
     2941 atgcaatggc agcaatcgtt gaaacaatga agaaagaagg cttatctgaa taatggttga
     3001 aatgctaaaa gggattgccg ctagtgacgg tgtagccgtt gcaaaagctt acctgctagt
     3061 tcaaccggat ttaacattca gtaaggcaac agtagaagat actgcggctg aagaagctcg
     3121 tttggatgct gcattagcta aatctactga agaattgcag caaattcgtg aaaaagcagc
     3181 gcaaagctta ggtgaagcag aagcgcaagt atttgatgcc catttgatgg ttctttcaga
     3241 tccagaaatg gtaggtcaaa tcaagcaaaa tattaaagac aacagcgtaa atgctgaatc
     3301 agcgctaaaa gaagtaacgg atatgtatat cggtatgttc gaagcaatgg aagataatgc
     3361 ttatatgcaa gagcgtgctg cagatattcg tgacgttgct aaacgtattt tagcgcactt
     3421 attaggcgtt actttaccaa atccatcaat gatcaacgaa gaagtggtcg ttgttgcaca
     3481 tgacttgact ccaagtgata cagcacaatt agatcgcaat tttgtaaaag cgtttgtaac
     3541 agacattggt ggacgtactt cacattctgc tatcatggca cgttctttag aaattccagc
     3601 aatcgttggg acaaaagaaa ttactgctaa agtcaaagaa ggcgatattt tggctgtcaa
     3661 cgggatcgaa ggcgatgtaa tcatcgatcc tacagatgaa caaaaagctg aatttgaaaa
     3721 agcaggtgca gattacgcag cacaaaaagc agaatgggaa aaattaaaaa atgcagaaac
     3781 tgtcacagct gacggcaagc attttgaatt agctgcaaac atcggaacac caaaagatct
     3841 agttggtgtc cataataatg gtggagaagc agttggtctg taccgtactg aattcctgta
     3901 tatggattct ccagacttcc caacagaaga tgaccaatat gaagcttaca aagctgtatt
     3961 agaaggtatg gaaggaaaac ctgtagttgt tcgtacaatg gacatcggtg gagataaaga
     4021 actaccatat ctgcaattac ctcatgaaat gaatccattc ttaggatatc gtgcattgcg
     4081 tatcagttta tctgaacaag gcgacgaaat gttccgtaca caaatgcgtg cattgcttcg
     4141 tgcatctgtt catggtaatt tacgtatcat gttccctatg gtagctacac tcaaagaatt
     4201 ccgcgcagca aaagcgatct ttgaagaaga aaaacaaaaa ttgataagtg aaggcaagga
     4261 agtatctgat acgatccaag tcggcatcat gatcgagatt ccagcagcag cagtcttagc
     4321 agacaagttt gcaaaagaag tagatttctt ctctgtaggt acgaacgatt tgattcaata
     4381 cacaatggca gcagaccgta tgaacgaacg cgtttcttac ttgtaccaac catataaccc
     4441 atcaatttta cgtttgatca aaaatgtaat tgatgcagca cacgctgaag gaaaatgggc
     4501 aggtatgtgt ggagaaatgg ctggagatca aacagctgtt ccattgctag taggtatggg
     4561 tctagatgag ttctcaatga gtgcgacatc tattcttaaa acgcgtagtt taatgaaacg
     4621 cttggacaca gcgaaaatgg ctgaacttgc tgatcgtgca ttgaaagaat gcgatatgat
     4681 ggaagaagtc gttgaacttg ttcatgaata tgtaaaataa acaacgtatc aaaagctggt
     4741 gtggcttgta aaagagctac gccagttttt ttgttctttt ttaagaaaac gtttttgtca
     4801 gtaagaatag acaaaaatag ggcttaagtt tgagtgacag tttgtaggct atgttatact
     4861 atataagtga agggggacgt aaggtgaagg tattattata ttttgaaagt gaaaagatgc
     4921 ttgcaaaatc tggaatcggg cgtgcgcttg atcatcaaaa acgtgctctg acggaagtcg
     4981 gtatctctta tacactagat gaaaaagaag attatgacat cctgcacatc aatacatacg
     5041 gaatcaatag tcataatatg gtcaataaag ccagaagaga aggaaaaaaa gttgtctatc
     5101 atgcgcattc tacagaggaa gatttccgaa attcctttat cggatcgaac cagctttctc
     5161 ctatcgtaaa aaaatatctg gtaggattgt accaaaaagc ggactatctg atcacgccaa
     5221 caccttattc aaaacagttg ttggaaagct atggtatccg tgtgccgatt caagctattt
     5281 ccaatgggat cgacttagag aaatatcacc ctgatccaat gaaagaacaa aagttcagag
     5341 aatatttcaa actgtctccg gatcaaaaag taatcatttg tgtcggtttg tttttcgaac
     5401 gtaaaggaat cattgatttc gtagaaattg ctaagaagat gcccgaatac acatttattt
     5461 ggtttggtca tgttcccatg tactcgattc ctcggaacat ccggaaaatt gtaaaagaag
     5521 atcatcctga aaatgtcctg tttcctggtt acattcgtgg agagatcata gaaggtgcgt
     5581 attcaggtgc agatctattc tttttccctt cttatgaaga gacagaaggg atcgttgttt
     5641 tagaagcttt agcaagcaaa caaaacgtac ttgttcgaga tattccagtg tataatggct
     5701 ggttagaaga tagcaagaac tgctatatgg ggaagacaaa cgaagaattt atccgactga
     5761 tccaacagat taccaatcaa gaactaccga gtacgacaca agccggttat caaacagcaa
     5821 aagagcgaag tatcaataaa atcggtgaag aacttaaaaa tgtatacgaa agtgtactga
     5881 atgaaaaagt ctctagcaaa ttaaaaaaag taaatcaggt caaggactag tttggaggcg
     5941 aaaaacatga aaatcggctt ttttaccgat acctactttc cgcaagtcag cggagttgct
     6001 acatcgatca aaacattaaa acaagaatta gaaagacatg ggcatgaagt ctatattttt
     6061 acaacgaccg atcctaatgc ggataagttg gaaaaagatg tgattcgaat gccaagtgta
     6121 ccatttattt ctttcaagga tcgaagagtt gttgttagag gcatgtggta tgcttatttg
     6181 gttgccaaag aactagaact agatcttatt catacccata cagaattcgg agcagggata
     6241 cttgggaaga tggtagctaa gaagttgaaa atacctctga tccatactta ccatacgatg
     6301 tatgaagact accttcatta tatcggtaaa ggaaaagtga tcaggcaatc ccatgtaaaa
     6361 tacttgtcta gattatttgc aaatcatacg acaggtgtag tttgtccaag tgaacgtgtt
     6421 attgatacat tacgcagcta tggtgtaata gcaccgcttc gcgtgattcc tactggtatc
     6481 gacattgaaa aattcaaacg ttctgatatc actcagcagg atatcagcaa attaagggag
     6541 tcactaggtc ttcaggaaaa ccatttgatg attctttctt taagcaggat ttcttatgag
     6601 aaaaatatcc aagccttgat taatgggttt cctcaggtca tctccgctta tcctaacgcc
     6661 agattagtta tcgtaggaaa aggaccttat gtagaagagt tagaggaatt gatctcagaa
     6721 ctgcagttag aagaatttgt gcagttcaca ggagaagtgg acaacaatga tgtagcactt
     6781 tattataaag cggcagatta ttttgtcagt gcttctactt cagaaacaca agggcttact
     6841 tatacagaag cgatggcagc cggaacacct tgtgtcgtgg aaggaaatgc ttatttgaac
     6901 cgattgttcg atcatccttc attagggaaa acttttgaaa ccgacgaaga ctttgcacca
     6961 gcacttattg attatatttc cagtgaagtt cctaaagatc caaaagtttt agaagctaaa
     7021 ctttatgata tttcatctac acatttcggc gaagctatga tcgaatttta tcaagacatg
     7081 gttgtttatc atgaagaaga gatgcg
//
LOCUS       DAXLPI010000116         7078 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXLPI010000116 DAXLPI010000000
VERSION     DAXLPI010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.118"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            282..1139
                     /locus_tag="ACF3T5_002263"
     CDS             282..1139
                     /locus_tag="ACF3T5_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFD3632818.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            1132..2034
                     /locus_tag="ACF3T5_002264"
     CDS             1132..2034
                     /locus_tag="ACF3T5_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFD3632819.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            2067..2417
                     /locus_tag="ACF3T5_002265"
     CDS             2067..2417
                     /locus_tag="ACF3T5_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFD3632820.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            2466..3389
                     /locus_tag="ACF3T5_002266"
     CDS             2466..3389
                     /locus_tag="ACF3T5_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFD3632821.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            3467..4360
                     /locus_tag="ACF3T5_002267"
     CDS             3467..4360
                     /locus_tag="ACF3T5_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD3632822.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            4398..4961
                     /gene="amaP"
                     /locus_tag="ACF3T5_002268"
     CDS             4398..4961
                     /gene="amaP"
                     /locus_tag="ACF3T5_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFD3632823.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            4975..5163
                     /locus_tag="ACF3T5_002269"
     CDS             4975..5163
                     /locus_tag="ACF3T5_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFD3632824.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            5176..6078
                     /locus_tag="ACF3T5_002270"
     CDS             5176..6078
                     /locus_tag="ACF3T5_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFD3632825.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            6110..6352
                     /locus_tag="ACF3T5_002271"
     CDS             6110..6352
                     /locus_tag="ACF3T5_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFD3632826.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(6608..6979)
                     /locus_tag="ACF3T5_002272"
     CDS             complement(6608..6979)
                     /locus_tag="ACF3T5_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632827.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         2581 a         1196 c         1406 g         1895 t
ORIGIN      
        1 aaatgagaat ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa
       61 cattcttaag aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa
      121 gccatgaaaa acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa
      181 tattcaatga aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg
      241 tagaacaggt tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg
      301 tagttcctgt ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg
      361 caactgcatt agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg
      421 gtctatcaga atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg
      481 caagtatcca attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc
      541 atatcacaac taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt
      601 ataagaaagt cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat
      661 atgatgagcc gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag
      721 ctttggaaag attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga
      781 ttgacattga tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg
      841 aaaatggcga tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag
      901 attgggaaca acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc
      961 cagcaccaaa tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg
     1021 ttcactttac aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt
     1081 tatacttaaa aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa
     1141 aaagaaatag cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta
     1201 tttgtctcag tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac
     1261 gatgatatcg attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg
     1321 aactcagatg tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc
     1381 gatcgtattc cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca
     1441 gaagttgaac gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa
     1501 ttatggcgtc ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat
     1561 caacgactgg aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg
     1621 atgctgatca atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc
     1681 atcgaacata atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta
     1741 catgaccgtt ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa
     1801 gcaaaaaaac atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct
     1861 tacattatcc atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg
     1921 aagaaatatt acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct
     1981 aaatatccaa aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag
     2041 acaacaactg aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga
     2101 cgttaattga aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg
     2161 atccatttct aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt
     2221 ttgtttcgga atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata
     2281 catctaaatt agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc
     2341 ttgacagctt ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga
     2401 actgcttctt cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact
     2461 actttgtgga tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg
     2521 tacttgatag tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg
     2581 atgatatttt cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg
     2641 agacgaaact agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc
     2701 gtatcgaaaa aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt
     2761 gtatcgatag aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa
     2821 actcgtttga aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag
     2881 aactaagaga gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg
     2941 aaaatgaaga actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata
     3001 ccttaagaga tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac
     3061 gtttagacga tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa
     3121 tgatcgagat ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg
     3181 acaataatct aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc
     3241 cggcgttgtt cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg
     3301 ctctaggctt tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc
     3361 atttataccg gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag
     3421 aaatttgttt tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa
     3481 attaatcgac ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac
     3541 accagcttta cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg
     3601 caataatcaa ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg
     3661 tgcagcagcg attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg
     3721 agaagaaaaa gatgcagagg aagtagcaaa atacgtgaaa gatgcaggac gtaagattgt
     3781 cttactacct tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt
     3841 gagtgaactt ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca
     3901 gtctattgct gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc
     3961 tatgtttgct ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac
     4021 aacaacttct gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa
     4081 agcttcaatc gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg
     4141 tgtaaatggt gtagcacctg gtccaatctg gacaccatta caactagacg aaggacaacc
     4201 ttctgattcg atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg
     4261 agaattagca ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca
     4321 aatttacgga gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc
     4381 tatgaggagg tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac
     4441 ttttgctatc tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc
     4501 agctagagaa gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt
     4561 ggcttgccgt tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc
     4621 caaaaacaat cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa
     4681 aagcaattga aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa
     4741 aagtaaatgt caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac
     4801 gaacttcagc actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac
     4861 aacacatatt gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag
     4921 aaccagctaa acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa
     4981 gaactatttt cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc
     5041 gttttatgga tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc
     5101 ggcgtcgcta tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct
     5161 taggaggaaa catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa
     5221 aaaccagtgt ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat
     5281 gaaaagaaca aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa
     5341 caaggagtag aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact
     5401 ccagaatcta aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag
     5461 caaaaacatc cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac
     5521 ggacaaggaa aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag
     5581 gaagattcaa tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga
     5641 atcgcaattg aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg
     5701 gcaggaaaac ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa
     5761 aaacaagtag cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc
     5821 tttgaagaaa tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg
     5881 atcgaagtca acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa
     5941 gaaacggtgc aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat
     6001 caaacagata aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca
     6061 gaaccacggg ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat
     6121 ttggtcactt atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg
     6181 ttcttcgatg gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca
     6241 agcattatta ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat
     6301 tggtgccatc attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt
     6361 ttaaaaagag gtcgtgaaaa agctgttctt catcaagtaa taagaacagc caaacaaaaa
     6421 tagcttctca tatttttcat ttggttgggc atattatcga agatgcaagc ttttgaacac
     6481 tgtttatacg gaataagagg ctgtgacaag acttttgtca cagcctctta tttatgcggg
     6541 caaattccca attagatttt taaaagtttt tccttaaatg tgtctttatt gatacagaga
     6601 agcttcttta ataaaacaat gcctccctaa attttcttga gataggaaag gtgaacatac
     6661 aaaataatac gaccactttg aaaacccaga tgatccacaa atggatcgta tgatactgat
     6721 gaagtaaaaa gcaatggatc agaaactcaa agagattgaa aaagacaagg aagaaataat
     6781 atctcttttt tactcttgtc tgttttagcc aataatagct cgtaaagtaa tctagacaaa
     6841 caaatagaag taaacaaagt ttcgtaagag cagcaatcgt atcatgttgc tctgctagac
     6901 gattgacgag caggaaaaat gttggtaatg gaatatagat cacgagtaat gaaagaaaaa
     6961 agtaaacaat aaatggcata ctattcctcc taattgtgtg agttcgccct cattttaaaa
     7021 taacgatatt tgtttgtgaa gctttttcgt gtcgtaacaa atgttatcta atgtattt
//
LOCUS       DAXLPI010000117         7023 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXLPI010000117 DAXLPI010000000
VERSION     DAXLPI010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.119"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            35..220
                     /locus_tag="ACF3T5_002273"
     CDS             35..220
                     /locus_tag="ACF3T5_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632828.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            725..919
                     /locus_tag="ACF3T5_002274"
     CDS             725..919
                     /locus_tag="ACF3T5_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632829.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            1110..1359
                     /locus_tag="ACF3T5_002275"
                     /pseudo
     CDS             1110..1359
                     /locus_tag="ACF3T5_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1603..2433)
                     /locus_tag="ACF3T5_002276"
     CDS             complement(1603..2433)
                     /locus_tag="ACF3T5_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFD3632830.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            2641..3975
                     /locus_tag="ACF3T5_002277"
     CDS             2641..3975
                     /locus_tag="ACF3T5_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFD3632831.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4016..4927
                     /locus_tag="ACF3T5_002278"
     CDS             4016..4927
                     /locus_tag="ACF3T5_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632832.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            4924..5778
                     /locus_tag="ACF3T5_002279"
     CDS             4924..5778
                     /locus_tag="ACF3T5_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFD3632833.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            5775..6392
                     /locus_tag="ACF3T5_002280"
     CDS             5775..6392
                     /locus_tag="ACF3T5_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFD3632834.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            6394..6912
                     /locus_tag="ACF3T5_002281"
     CDS             6394..6912
                     /locus_tag="ACF3T5_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFD3632835.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
BASE COUNT         2108 a         1206 c         1404 g         2305 t
ORIGIN      
        1 ctataataaa agttatttaa ggaagtgtct atgaatgagt aaaaattcta aaggattcct
       61 aaccatctta ttagctttta tagggtatat gttggttggg ctgctaaaat catacagtaa
      121 tgagttgctg aatttttcaa catttattaa tgatacatta gttccaagtt tgtttttcat
      181 tgtatttttt gctgttggtt attttataat aaagatatga tgactaattt agctcaacta
      241 ttccagcgtc agctgaagat attgcaattc ccgattcctt aatcatttat ctggatgaga
      301 cagaaagtga agttgcaaca gatgtgcatg tagttgagga taaaaatgat agtttaaaaa
      361 ttaatagttc agatacttta ctaagagtaa gtcctatctt ttcagctgtt atttagatgc
      421 aagtagcaac taagttaggt agtactcctg caatggatag gtatttgtgt gttcgatata
      481 taagtggtcg aagtatcaag gttatgtata ctggatggga gatagaagag ttaaaaattg
      541 gaggtcaccg ccacaaatgg aatttagatt tagaggtacc ttagatttgg tatagttatt
      601 aaacacaaga tggcctggtt tgtagggaaa gtcgaagagt atttttccac ttctgcgccc
      661 ctttaatttt tctagtaatg ccgtgaattt gtgattaatt ggaaatatga agaaaggaga
      721 gtctatgaaa acaacactag ttctattcta taaaaagcat ccttatttta cacttctgat
      781 aaatatattg ttggctagtg ttatcgggat ttctgttgaa tatctaataa ataaagattt
      841 cataggttct tgtttttata cggctttatt tcttggactt cttgaagcat tttccattta
      901 taaaaaatca aaaaaataaa atagattgcc gtaagagatc tttttcattt catttttttc
      961 tgaaaacccg ttgtttctgt cagtgaagca gcgggttttt agatttattg tctttagacc
     1021 taattgagcg cgccgagcga ttgaaataca aaaccacttg ctatgcgggt ggagcaataa
     1081 agggaaatag tttagcccgt acaaagtaga tgtgtaaata ccacattgta ttcacaccaa
     1141 agtatagaag aaaaataatt tataatcaat atcgagccaa tattcaggag atcatcaaga
     1201 gattgtgcaa atctaaaata taaatttggg tatcgacact tttgggtgga aggatactat
     1261 gtgacaaaag taggacaaaa tgaagcgacc atcaaaaagt acattcaaga acaagaaaaa
     1321 catgatagtg cgttagataa gctgagtgta cgagagtaag acgatccttt taagggctaa
     1381 ggagtagtac ataaaccttt taagaggtgg gcaaaactgg caaaggcaat atggcttgaa
     1441 cataaaatat gaaagccagc gtctttaggc gctggccggt gatttgagct tatagctcta
     1501 gagaaaacca cccttttgaa gggtggttat gattaatgac tttagtcagt tttgctcaca
     1561 aagcgtgtgg aacaaaaaac gggtgtttct acgggtagat atttatttct tgaatggagg
     1621 tgtcccatac atttttggtg ttactggttc taattttggt gcagcgcctt cgatcttagg
     1681 ttctttcaac cattgatatt cgccttcgcc atccagtgct ttgccactag cccaagaacc
     1741 ttcactactt tggtctccct ctgagaagtt atagaagtca taagcaatgt cttttcgttc
     1801 tttgtcacgc gggaaagtac ttgggactaa gattgaacct tctttttctt ctaattcttt
     1861 cactgcacat tcccatagat tttgatgata gctatctctt gcaaggagaa cagacagcat
     1921 atcttttacg ccacgatcat cgatcatttc gtaaatacgt gctacttgaa gtcttccttg
     1981 tgattcggca tttaaattag ctctaaagtc tgctaacaag ttgccactcg caatgatata
     2041 ggcaccgctc catggattgc ccacgctgtc agcaggacga ggtcctaagc cagctacgat
     2101 cgcgtgctgt ggattcattc cagagataat ggcaccgatt gttggatctt tttgcatcgc
     2161 tgcttcttga tcttcaacag gcgcaccctc taataaacga gcaaccattg tcgccaacat
     2221 ttccacgtgg ccgatttctt ctgttgcaat gtccatcagc atatctttat atttttcttc
     2281 tcctcggcag ctccagcctt ggaacaggta ctgcatagct acactcatct cgccatattg
     2341 gccgccgatc agctcctgca gatatttcgc gattaatgga ttcggacgat ctggttttgc
     2401 ttcaaactgc aattctttct ggtgtctaaa catcaaaatt cctcctaatc tatttttaat
     2461 acattctcat tctgctacga aagattaaaa aattcaaaga aagcgctcgc gaattatata
     2521 aagtattggt atcattaata atttttttag aatcatacct tataaattgt tattttcaga
     2581 acaaaatgat atcgttatat taaaaccggc atcggttaca ttaaaatttg gaggtaaaat
     2641 atgaaaaaat tattgtttac agggttgctg gcaagttcag cattgttttt attaggagga
     2701 tgcggatcaa gtaatgcaag cgatgccagt tcaagtgcag caaattcttc tgaaaaaaca
     2761 agtgatggaa gaaaaaatat tgatttctgg tccttttggg gatccggtgc acgaaaagaa
     2821 gctatcgaag aaatcattga ggatttcaat caatcacaaa acgagatcca tgtaaattat
     2881 aagtatcagc catggggtga catctggact aaatctttat ctgcaataac agctggaaat
     2941 gcacccgatg ttattgtaca ggacataaat tccgttgccc aaagagcaga agcacagcaa
     3001 gcaactaatc tctccaaata tgtagaaaaa gacacttcta aggattttta tccgcagctt
     3061 tgggatacag tagaatatga aggtgatcct tatgcgatcc cattcaatac agatacacaa
     3121 gtcatttttt ataataaaaa gttatttaaa gaagctggaa tcagtgaaca agatctaccg
     3181 acaacttggg atgaattaga agaaacagca cgtaaactag ataaaaaaga aggaaaagaa
     3241 tttacacgca tcggttttta tccattatgg aatttagggg ctgatgtatg ggcgctaaac
     3301 gcagataacg ggactagttg gtttgacaaa gatgaaaaag tcaagattga tactaaaaat
     3361 aaagtggaag ccttggagtg gatccaagac tggcaagatt attatgggaa aaatacgatc
     3421 aataaattag aagccgaatt tgggtctggt gtatctgatc catttatttc cggattagta
     3481 gccatgcgtg cccaaaatat caattattac tctagtttga tggaaaatgc accggatgat
     3541 tttgattttg gcgtgatgca gctcccagaa aaagaagaag gttcaggcca ttggtcgtgg
     3601 ggtggcggat tcgtcttgga agtaccatac ggagcaaaag atcctgaggc ctcttatgaa
     3661 ttcatcaaat atctttcttc tccagaagta caagaaaaat tcggcgaaaa aagttttgat
     3721 atcatggcga gcaagacagc caatgagaat ttagtgaata atgacaacct aacggataaa
     3781 ggaaaaatga tctatcaaat ggcggacgaa aactttaaaa atacggtgat cacaccagtt
     3841 cctttagatg ctccggatta cacgacatta gtgaatgaac agattgacca gattctttta
     3901 ggaacaaaat caccaaaaga aggattggca gatgcacaaa aagcagtaga aaacttagtc
     3961 aagcaaaatc aataatccat ttttgactgt ctaggtacag aggaggcaag aaaatttgaa
     4021 gcgtttatct atccgaaaga aaaaagaagc aagatgggcc tatttattta tctcgccttt
     4081 tattatcggg ttcttggtat ttatgctagg gcctatgctt ttttcagtta ttggtagttt
     4141 caccgattat aatttgacat ccaagatgaa tttcattggt ctagcaaatt tcaaaagaat
     4201 gttcgttcac gatgatttat tttggaaatc gttatacaac accgtttatt tcgtcatctt
     4261 caatgtaccg ctgacgacga ttttcagtgt gtttttagcc acagtactca atcaaaaaat
     4321 caaaggaatc agtgtattcc gtacagcttt ctatttaccg gcagtcttat caggagtagc
     4381 agtctacatc ttatggatgc agctgctttc tccatctgca ggattgatca acacatttct
     4441 gggctggttc catatccctg gaccagcttg gctgtttgat ccacaatgga cgaaacctgc
     4501 gttgattttg atgaaaatat ggagttctgg aggagcaatg ctactttact tagctacatt
     4561 acaaaatgtt cctagatctt tatatgaatc agcagaaatc gatggagcag gcgtttgggg
     4621 aaagttcaaa agtattactt tgccattaat cactcctatt attttttacg atgtgatcac
     4681 ctctacgatc ggttctttcc aaatttttca agaagcttat gtcatgacga aaaatggaac
     4741 aggcggacct gcaaattctt tgctttttta taatctgcat atgtggaaca aggcgttcgt
     4801 agcttttgat atgggttatg cgatggctat gtcaatgatc ctgtttctga tcgtgttggt
     4861 tctgacgttt gtcaatttga agcttgcacc taagtgggtc tattattctg gaggtgacgg
     4921 caaatgatgg attatttcaa aaagaccagt gtgaatcggc aaacaaaaaa aatactaagt
     4981 tatgtcctca tgacagtaat aggaatcatt ttgatcattc cattgctttg gatggtgttt
     5041 acttcattga aaccaatgga agaaatcgtt cgttatcctc cgaccttttt cccagaaaaa
     5101 atcgtatggg aaaactatct ggatacgatt gccgcctttc ctttttggcg ctatgcaaga
     5161 aatacattat tcatcactgt gctagtagtg atcggtaatg tcctttcaaa ttcttttatt
     5221 gcttatggat ttgcgaagct tgattttcct gggaaaaagc tgatgtttgc tttggttctt
     5281 tctacgatga tgatcccggg gtttgtaacg atgatccctc aatatgtatt attctccaag
     5341 atcggctggg taggtactta tttgcccctg atcgttccat cgttcttcgg caatgcgttc
     5401 aatatatttc tgatgcgtca attttactta tcgatcaata atgaattgat cgaagctgct
     5461 gaaatcgacg gagcgaatca tttgtatatt tggagtcatt tgatgcttcc attgacgaaa
     5521 cctgccttga ttaccattgc gatcaattcc ttcaacgcag cttggaatga tttcttaggt
     5581 cctctgttat atatccaaga tcaagaaaaa tacacgttac agattggctt gcaagttttt
     5641 cagaaccaag caacgacaca atggaattat ctgatggcag gagcaacact tgtcttgatc
     5701 ccgacgattt tgctgttctt ttttgcacaa cgttatttca ttgaagggat ggatttgaca
     5761 ggaggttcaa aaggatgatg aatcgatcga ttggtaaaaa agccaatgat tatttcttag
     5821 aaatcattga atggattcca cgtcttatcc atttacagct actatggttc ctgtgcgtct
     5881 tgcctgtttt cacattagga actgcaagtc gaacagttct gtatatgatt tatcattaca
     5941 aaaaaaatga agagaaaaag ctcggttcac tattctggaa aaagtttcgt gagaacttct
     6001 ctctttatgg aaagcaagat agtttagcaa gtgtttattt tcttcttcta ttgatcgatt
     6061 atcagatttt ccgctattgg ggtgggggaa ttggctatac attgatgtat actacgttgt
     6121 ttttgcttct attaagcgcg cttctcttta gttacaaaat cttgcttcaa ctggaacaaa
     6181 aagaagcttc ttggattact gcatttttct tgttttttaa tgatttcaaa agcgcgcttt
     6241 tctatttggc aggcacgttg atccttcttg tcgcattctg gtttgcagga ccaatctatt
     6301 ttgctttatt aggtttcagt ttcttgtttt attttcaagt cctgcttttt atccatcgta
     6361 cgcaagaaaa ggttcttaaa aaggaggaat aacatgttgc gtttgattgc taaaatcgat
     6421 gatagtatta tcaacaagtt cgccaagcaa cggaaaacgg atagtcgaaa aatcaatttt
     6481 ttgaaattta tcagtaatat ctctaatggt ggttttattt ggttgctcct tgctgctgtt
     6541 ttagtcttga tacctgatac tagagaatat ggtctagcca ttcttatcgg cagtttgatc
     6601 tatttttttg ttggtttgct ttttatcaaa aaaacgacaa gacggaaaag accttatgaa
     6661 aaaaatcgtc ggcttgtcct ttacacaagt aaaccgaaga cttcttcttt tccttcagga
     6721 catacagcgc tagccttttt atcagtagct gcgttgttcg aattaagtcc agttgcagga
     6781 atattagcct ttttatttgc ttgtttgatc gcttattcga ggattttttt acttttacat
     6841 tatcctactg acattttagg aggagctatt ttaggaagtg ttattggcat cctatgcgct
     6901 gccgttatct gaattaattg tacgaataaa gcatgagtgc gtaaatgcgc atttgtgctt
     6961 tatttttttg tttcgaggaa agcgtagttg tttattcatt tgtcttaata acttaagttt
     7021 tgt
//
LOCUS       DAXLPI010000118         6904 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXLPI010000118 DAXLPI010000000
VERSION     DAXLPI010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.120"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..173
                     /locus_tag="ACF3T5_002282"
     CDS             <1..173
                     /locus_tag="ACF3T5_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HFD3632836.1"
                     /translation="RNIFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLF
                     ATLLNYSIYFLN"
     gene            complement(281..1507)
                     /locus_tag="ACF3T5_002283"
     CDS             complement(281..1507)
                     /locus_tag="ACF3T5_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFD3632837.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(2261..2461)
                     /locus_tag="ACF3T5_002284"
     CDS             complement(2261..2461)
                     /locus_tag="ACF3T5_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFD3632838.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(2595..3203)
                     /locus_tag="ACF3T5_002285"
     CDS             complement(2595..3203)
                     /locus_tag="ACF3T5_002285"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFD3632839.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(3288..3758)
                     /locus_tag="ACF3T5_002286"
     CDS             complement(3288..3758)
                     /locus_tag="ACF3T5_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFD3632840.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(3806..4720)
                     /locus_tag="ACF3T5_002287"
     CDS             complement(3806..4720)
                     /locus_tag="ACF3T5_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFD3632841.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            4866..5669
                     /locus_tag="ACF3T5_002288"
     CDS             4866..5669
                     /locus_tag="ACF3T5_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFD3632842.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            5847..6794
                     /locus_tag="ACF3T5_002289"
     CDS             5847..6794
                     /locus_tag="ACF3T5_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFD3632843.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         2149 a         1368 c         1046 g         2341 t
ORIGIN      
        1 taagaaatat tttcattgca gcaatcgata ttacactttt agttattttc ttgtcagtgc
       61 ttatgtatca gttgtggcta catcatcgcc tggcaatgtg gctcatgatt ccttattatt
      121 tatgggtact attcgcaact ctattgaatt attcaattta ttttttaaac taagcgctaa
      181 aaagagacgt cctgtaattt aacacataga aaaagtgcca cctaagttag attttttgtc
      241 taatttaggt ggcactcttt attaaccaaa ctattttttt ctagtaccca cttgttgttc
      301 catactcatt attctggtca taaccataac cattttgttc ctgtccagcg taaggatcat
      361 aactttgagt atcttgactt ggtgtatagt actgacttgt atcttctgtt tgctgataat
      421 aggtattcgg atcttggtac tgatcatatg tgttttctga ataggaagaa taatcggtgt
      481 acccttcact attcgatcca tcaccgtttg tcgaatcgcc atcttccgaa tcatcaaaga
      541 acatcattga agcattctca tttttcaaat taggtaatgt atcgcttgtt tttatattta
      601 attgtttctt cagttcattt tggatcgaaa gaagatcatt ttttcctaaa atttgatagt
      661 agatatcatt gatcatttgt ccttcgcctt gcaactggtc ttgtttgatt gtttcaaacg
      721 caggggtgta gttcgtcgct acatcaagca tgtcatccca atctaagtcc gtcgtcacat
      781 tgttctcaac tgcttttaaa atttttctat aattgcttac gccatctaaa cttaaaactt
      841 tattgacgat tttcgtcact acttcccgct gacgacgttg tcgaccgata tcgccttctg
      901 gatcttcgtg tcgcattcga gaataggcta aacctttttc tccattcaat gtttgttcac
      961 ctactggtac atggacacca tccaaagtaa agtcaatttt attattcact ttgatgccac
     1021 cgaccgcatt gatcaaatct tccatgccat ccaaattgat cgttacataa tgatcaatag
     1081 gaatatcgag cagttgctca attgaatcca tggccatttc gacaccacca aatgcatagg
     1141 catgatttaa tttatctacc gttccataac ctacgatatt cgtataaata tctcggtcca
     1201 aactgatgat cgttgatttt ttttgtttcg gattaactgt tactaccatc atcgtatcag
     1261 aacgtccttg ttccgttctt cctaatccac cggtatctaa acctaatagc aaaaccgaaa
     1321 aaggttcttt gtcatcaatg cttactctct cagaacgttt ggaggatatt cgattagagt
     1381 ttttagaaat tttgtttact gtttggctcg cttctcccat aaatctgatt ccgtatgctg
     1441 ataatccggc aatcagcaaa acaaataccc ctaacaaggt taatagaact ttttgccatc
     1501 gcttcataat acacctctgt ttcacatttt ctaaaatatt gtctcatata actgttcaat
     1561 cagcaaattc actttttaat cataagaaaa ctttaaaaaa catttttgtg taacgattac
     1621 tattttttaa aatttctcaa aattcttaac tatcatacca taccgtgaaa aagatcgaaa
     1681 gaccgaatac acgcagttcg caatcgtttt atcttttttt acgtattttt tccagaaaaa
     1741 gcgaacaaca aggtattttc caaataatcg tgcagtaaaa tgatcgcaca tacctttccc
     1801 atctttttaa cagtctttgc ctaccgttca gattatcatt cgatttcacg aaaatttctt
     1861 cctatgactg gtccttttgt taaacccatc ttctattcgt tttatttcca gctcactgca
     1921 tggctcttaa aatgactcga actagtgatt tcattaccat catcttttaa tggatagata
     1981 tgctcctctt caaagtaaaa cgattttctt ttaaagtaat agaattgaca tggtaatgag
     2041 tatgtatact agtggtttca caaatggtct tttattcaac atccaaaaac tgaaatttct
     2101 ttaaaatcca ttactaacga agaaaatgtt ccttgcttga taattagtta ggacatgtca
     2161 agtgaacaaa tgcatttact tagccaaaaa atctaaaaac ttcataaata gacaaaaaga
     2221 cacgactctc taagaagtcg tgtctttatt caacaaatta ttataattta gtcacttcag
     2281 cagcttgagg tccgcgtgca ccttcaacaa tcgtgaattc tacttcttga ccttcttcta
     2341 ggctcttgaa gccgtctcct tgaattgctg agaaatgaac aaatacgtca tctccgccgt
     2401 ctactgtaat aaacccaaat cctttttcat tgttgaacca tttaaccgtt ccgtgttcca
     2461 taaatgtata gcctccaaaa aagttcttgc aagtaacctt gcaagaacat tatacccaat
     2521 ttagaaaacg ctagcaagct ttcgctcttt tattttccaa attattttta agactgatat
     2581 gacggaggaa atggttattt tgctgcttcg aaacgtttgt ttacttcatc ccaattaata
     2641 acattccaga atgctgcaat ataatctggt cggacatttt tatatttcaa ataataagca
     2701 tgttcccaaa cgtccaaacc tagaattggt gtctttcctt ccatcaatgg agaatcttga
     2761 tttgcagtag aggtaatagc taattttcca ttttccatta caagccaagc ccatccagaa
     2821 ccaaatcgtc cagcggctgc ttttttgaat tcttctttaa aagaagaaaa atcaccaaaa
     2881 gcttcattaa tcgcttcttt tatttctcct gtaggttcgc caccagcatt tggtgccatg
     2941 atttcccaga aaaatgaatg gttagcatgt ccgccaccat tgttacgtac agctgtcttg
     3001 atatctgttg gaatagcgtc catatcagat aataattctt ctattgtttt ttcgcctaat
     3061 tctgggtatt tctcaattgc tgcatttaaa ttcgtcacat aagtattgtg atgcttatca
     3121 tgatgcagat gcatcgtttc ttcgtcaata taaggttcca atgcgtcata tgcataaggt
     3181 agatctggta atgtgtaagt cataaaaaaa ttcctcctca atgttttctt tataatgtct
     3241 acattacaaa gagtaacaaa gagaaggaaa ttcttcaatt gttatgccta gtcgtctttt
     3301 tcactttgac ctacttcttc ttcaaaaacc tcagtttctt taaaaataaa caacttgctg
     3361 aaaatatagt tcgctaggat aacaataatt tgagtgatga ttttcgccac atactcattg
     3421 ctgttcataa cattgatcaa caataccatt gcccccatgt ccaatccgta agaaaggata
     3481 cggtagaaga agaacttacc gcattcaata agtgcatgtg taaaattcgg tgattttgaa
     3541 tggaataccc aaagtttgtt cgttacaaaa gcaaacaata cagaacaaaa ccacgaaata
     3601 gaattggata aggccatact taaatcaaaa tattggcgtg cgagagcaaa actaacaaaa
     3661 tttacaatcg tagccaaacc accaaaaaag agatagacta gtatctccca atatcctttt
     3721 gcttctaaat atccttttaa ctgtcgatac tgcttcatga gccaacctac cttattaaaa
     3781 attcttattt atcatactct atatttcaat aactttcatc tttatttaaa aaaattaatt
     3841 ttttttgatc ctttgccttt gtctctgctt ggatcgtcac atgtgtaata ccatatttta
     3901 attttaataa agaaattata cgaggatata tcttttcaat ctcgcttagt agctgatctt
     3961 caaaatcaat atgtccgtcg aaaataatta tcttttcgct atactgccaa atatggacat
     4021 gatgaacatt ctttacccca tcgattgctt caatttcttg ttccattttt tcatagtcaa
     4081 tagcaggtgc actttgtatc aaaatagcgg cagctttttt gatcaccatt atcgcttcta
     4141 tcaatatata tatcgaaatg atcagtgtaa taactggatc gatccagtag atatcgaaaa
     4201 aacggatcaa aattgctccg ataatcacac ttaccgaaga cagtgtatca cttaacaaat
     4261 gcaaataact agattttata ttcaaacttt catctgcttc ttgtcgtaat aataatactg
     4321 agaacaaatt cgcgagcaat ccaataactg ctactgttag catcaaatga ctatttatct
     4381 tttctggtga agtgaaccgc ttgaatgctt caacggttaa aaacaaggat aatatgatca
     4441 atatgaatga gttgatgaaa gcactcaata tttctaaacg ctggtagcca tacgtacgtt
     4501 ttttatttgc ttcttttcgg gccatttttt gtgccccgta agaaaaaata attgccaatg
     4561 tatcactaag gttatggaaa gcatctgaaa caagagacaa actccctgac ataatccctc
     4621 caataaattc tgataaagtg atcaccgcat tcaaaaccac tactccaaat agtttttttg
     4681 atgatctctg tggctgtgta ggcactattc cttcctccat tttatttata ttggatagtc
     4741 tttagacaag aataacattt ttttgatatt tcaccaatca ttcagatgtt tccctaaagc
     4801 acttgtattt cacttgatac ttcgctagaa ttgcaagtga tgacactact agatgaggag
     4861 tacttatgac aacaaaacaa ttaattatca gttcctttat acgattcaaa gacctaaagg
     4921 agacgagaaa tgtcccattt tggaaagcca ttgtctatct tttagttttg agtataatta
     4981 tggctttgcc aatctctttt caagtttttc aagtcttaaa caacattcaa ttggatgggc
     5041 aaaagattgc tactcgcatt cctgatttta caatccaaaa cgggcagctt caaaccgaag
     5101 aaaaagaagg attcatttac caaacaaact ccattatctt cacatttgat ccagagggaa
     5161 aacgaacaga acaggacatc tctactgatt tgatggggaa ttttgttagt gtcgggatgt
     5221 taaaaaacaa acttatcata gctcttccta atacaggcac gacttctgct ttgttgaaca
     5281 ataaccaatt agaacttccc tacaccaatg aatctttaaa aaacttaaca ggtaaggagc
     5341 tccgttcttt tttaagtaaa gcatctatac cactttggat aaaagtcctg acatttatat
     5401 tttctattta tcctagtttt ctgaatctgc tgatcacttt gctatttacc aacattgctg
     5461 cttttctcta tgcacgcttt cgactaacaa aagctacttt tcttgattgt ttaaaaacat
     5521 tgatctattc aatttcattg cctactataa ttgcaacaat cttgatggtc tttcttcctt
     5581 cttttgacac aagcgccttc attgcttttg caggaatctt tatttttgct caagctgtca
     5641 aaggatgggc aaaaatatcg atcaattaag aaataaagac aactcgttat ttctacttca
     5701 atagaccctt ttagaggttg tgacattttt ttgtcacagc ccctttttta tttgaaaaat
     5761 atttctttct caaaaaacaa gcaaggcgct tcaaagttct tcttgtatgc gctacactta
     5821 gagtaaagaa aggcgggaaa ttaactatgt cagaaagaat catttatgca gatcgtccat
     5881 ttcgtgatga atttatcgaa gaaatccata ctatagcgcc tggttatatc tttaaaacag
     5941 aattacaacc agaggatcta caaaatgtag agatcagttt gggctggaac tcagactacc
     6001 aagaaaaact attagcctct tctaccttga aatgggtcca atctatctca gctggtgttg
     6061 atcagctacc ccttgagact ttttcaaaga aacatatttt actttcaaat ggtagtggca
     6121 tccacagtga atcgattacg gagcatgtct taggaatcat ccttgggtat tctcgtggtc
     6181 tttttcaagc acaaaaagca cagagtgaca tgaaatggtt aggttcatcg gtccattacc
     6241 aatctatcca aggaaaaaaa atactaatca ttggaactgg ccatattgga aaaatgattg
     6301 caaaaaaatg caaagcatta gacatgaact gctatggaat caatacatcc ggtcatccag
     6361 cagaaggaat ggaaaagact ttttctttaa atcaactaaa ggaagttctt cctgaagtag
     6421 atttcgtagt caatatctta cctctaacgg aggaaacaac tgggctcttt gatcagcata
     6481 tgtttgaaca ctttgatcca aaatctgtat tcatcaatgt tggccgtggt gcttctgtta
     6541 agacacaaga tctggtgcag gctttaaata accagcaact ttcctttgca gcattagacg
     6601 tttttgagga agagcctctt ccacaagatc atcctctttg gaaaatggac aatgtactga
     6661 ttacttccca cattgcaggt ttgacacctg attttcaaaa gaaattaatg gctatttttt
     6721 taaccaatct gaaaagctac tttgccaccc atgagcttca aaccaatcaa gtaaaattgt
     6781 ctgcaggata ttaatagctg aaaacaatat tttcaaaaga acaaagagaa ggtgacaaaa
     6841 ctggctctat aatttatggg actgatgaat cagaattgat tcttcagccc catttttatt
     6901 ttag
//
LOCUS       DAXLPI010000119         6834 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXLPI010000119 DAXLPI010000000
VERSION     DAXLPI010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.121"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            1..873
                     /locus_tag="ACF3T5_002290"
                     /pseudo
     CDS             1..873
                     /locus_tag="ACF3T5_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            886..2496
                     /locus_tag="ACF3T5_002291"
     CDS             886..2496
                     /locus_tag="ACF3T5_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFD3632844.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2573..3232)
                     /locus_tag="ACF3T5_002292"
     CDS             complement(2573..3232)
                     /locus_tag="ACF3T5_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFD3632845.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3515..4180)
                     /locus_tag="ACF3T5_002293"
     CDS             complement(3515..4180)
                     /locus_tag="ACF3T5_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFD3632846.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4327..4932)
                     /gene="dhaL"
                     /locus_tag="ACF3T5_002294"
     CDS             complement(4327..4932)
                     /gene="dhaL"
                     /locus_tag="ACF3T5_002294"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFD3632847.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(4960..5970)
                     /gene="dhaK"
                     /locus_tag="ACF3T5_002295"
     CDS             complement(4960..5970)
                     /gene="dhaK"
                     /locus_tag="ACF3T5_002295"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFD3632848.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5945..6340)
                     /gene="dhaM"
                     /locus_tag="ACF3T5_002296"
     CDS             complement(5945..6340)
                     /gene="dhaM"
                     /locus_tag="ACF3T5_002296"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFD3632849.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6494..6814
                     /locus_tag="ACF3T5_002297"
     CDS             6494..6814
                     /locus_tag="ACF3T5_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFD3632850.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
BASE COUNT         1806 a         1443 c         1321 g         2264 t
ORIGIN      
        1 atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt aaaagatgtc
       61 tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg ttctggaaaa
      121 tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac ggcaacgatt
      181 tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc ttatgttcca
      241 ggcgatatct ctttgtaggg aaatttgact ggaggagcga tcatcgatct cttcatgaag
      301 cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt ctctcttgat
      361 cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg cttgattgct
      421 gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg tttagatcct
      481 ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg caaatccatc
      541 ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt cgtgatcata
      601 cgcgaaggaa cagtcgtaga agaaggaaca ctcgatgaat tgcgtcattt gactcgttcg
      661 accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa cggtgtccat
      721 gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc tttcgatgtg
      781 attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc accgactctt
      841 gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac tgataaattt
      901 tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa gatcctgata
      961 tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga agaattgact
     1021 tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat gattgcaatg
     1081 gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat gtatgcaaat
     1141 gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca cgtggtgagc
     1201 caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt tcgagtcgga
     1261 cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt gcttttagca
     1321 atatcgatca gctgtacgat gatttttttc caagcagatt cgatcactgc agaaggtgct
     1381 tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt tgcactgttt
     1441 gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc gtttttaggt
     1501 ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc gatgtggaat
     1561 cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat tcctttgctt
     1621 tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga ggaacatcga
     1681 gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa acgttctctt
     1741 ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag ttggcttatc
     1801 acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca acagttttta
     1861 gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat tgaaaaaaac
     1921 tttacaagca cgatcatggt cgtcttgatt tgtctagctg ctattttacc aattgctagc
     1981 atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt cgcaacaaaa
     2041 atttctagga accaacttta ttggaccaat atgttactag ctattctatt tgctatattg
     2101 gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat gaaggatgcg
     2161 aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc tgccgtttta
     2221 gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatggg taaacttgct
     2281 tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt ggatttacca
     2341 gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa cgattctttt
     2401 gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt ccttggttct
     2461 attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta caatttcatg
     2521 ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt atttacaagt
     2581 cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt acataaccat
     2641 tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg ataaatgcag
     2701 caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct ttagctaagt
     2761 caagcagata gacagcgact cgttcttcta ctttttccat catcaaaaaa gttgtctgct
     2821 gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta agggcgggat
     2881 aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc gttttttcta
     2941 gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct ccctcatatc
     3001 cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat tgatacactt
     3061 tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca agaatctgtt
     3121 ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga tcttgctgta
     3181 ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc ataatctctc
     3241 ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc aactgataat
     3301 tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag agtggatttc
     3361 tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat tcctatcaat
     3421 tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa gccgagagta
     3481 aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc gcacctagct
     3541 tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca acgtgttcga
     3601 tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc atcgcttttt
     3661 tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag tggtcgtcaa
     3721 gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt ttgctccact
     3781 ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt tcttcgcttt
     3841 tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa actgctagac
     3901 aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga ctgcgtaaat
     3961 tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata tcaggcaaaa
     4021 gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta tattctttct
     4081 tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat gcgtagattg
     4141 gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct ctttccgtta
     4201 tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga aaaagctgtt
     4261 ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac cgcagtttta
     4321 ttgaacttaa ggttgattga tcacatctaa aagcgttgaa aataaaatgg cactagaagc
     4381 agctcctggg tcgatatgtc cgattgcacg ctctcctaga taggaagctc gtcctttttt
     4441 agctaccaag actttggtgt gttcttttgc ctggtcaatg atttcttttg ttaaattccc
     4501 tgccttcaat gcctctgtga ctggcagcca aacatctaac atcgttttat cgtcagcttc
     4561 tgcctttcct cggctttgaa tacccgctgt tccctgctcg atcacttcgc ctaatttttc
     4621 ttgagaatca atagaatcga cctcttttgt cgctttcatc atgttcatga atgcagatcc
     4681 gtataacgga ccagaagccc caccgacttt cgatatcatc gccatagaga atactttgaa
     4741 tgtatcgctg attgtttcag gatgatcggc ttgaaaagct tcttcataag ctgaaacccc
     4801 tcttcccatg ttatttccat ggtctccatc gccaatcggg gtatctagat cactgagata
     4861 ttgtttgttc tgattgattg cctctgcaaa ttgcgtgagc cattcttgga tttcttgaac
     4921 ggttagtttc atattatcac ccttttatct tgagattttt taccatgaaa tcgtctgtac
     4981 attgctttct aaagaatctt tccattgtgt ctccgtcaag tcgatcagtg ttagagaaag
     5041 accttgcata tctagtgagg tcataaaatt accgactttt cggaaaacta cattgacgcc
     5101 tctttcttcc aacagagcta aaacatcatt catgaacacg aactgttcca tcaaaggcgt
     5161 cccgcccatt ccgttgacta gtacacctgc ttcttttggt aaattcgagt aatcatctaa
     5221 aatcttagta acaagttcct ttgccagtac tttagaaggc tgcatctttt ctcgacgata
     5281 gcctggctca ccgtggatac caacaccata ctcgatctca tcttctccta gttcgaatcc
     5341 tggtttccct acttctggta cagttgctgc tcgaagcgct agaccgatcg tcttagttgc
     5401 ctgtacgacc ttttctccca gttctttcaa ctcagctagg gaagcaccgt ttcttgctgc
     5461 atcgcccacg attttgtgta caaggacagt acctgcaact ccgcgttttc cagcagtata
     5521 cgtactattt tcaacggcga tatcatcatc tacgatcaca ctttctactt caatatcgtc
     5581 catagcagct aattcctttg ccatatcgaa gttcaatgca tcccctgtat aattcttaac
     5641 gatcagcaaa actccttttc cactatccgc ttccttaata gccgtctgaa tctgatctgg
     5701 agttggagaa gtaaacacat caccaagcac cgctgcactt aacatccctt cccctacaaa
     5761 cccggcatgg gatggttcat gtccgcttcc tccaccagaa accaaaccaa cttgttccgc
     5821 tttctgattc ttggctacaa cacgggaaga ttctacacga tgaacaagtt ctggatagct
     5881 ttttactaat ccttccagca tttcttcgac aatattgctt ggatcattca tgattttttt
     5941 caatttattc ccatcctttc tttccggcat tttgtgcttc tgttaagatt tgctgagtat
     6001 catctgttat accggcagtg atggctgctg caaaagctcc ttccactaaa ggcgcatcca
     6061 ccagatgata acgttgctgc tgctcttctt ccaacatgtc ttttgccagt tccgagttca
     6121 ataccgcgct accgatatcc gcaaaaacaa aataactagc tgcatcgctt gattcattga
     6181 ctgcttctac gattttcatc gggtctgatc cgatctcgcc atttattccg ccgagagaaa
     6241 aaatttgcac ctcttcggat ttttgcatct gctcaatcat ttctttaata ccatctgtta
     6301 ctttttgact atgagaaata agcaaaatac tttttttcac aagaatcttc ctttctttta
     6361 catagttgct aacgattggt ttcgctttca ttaatgaaca attcaagtat ataataaatt
     6421 tctaattttg ggaaaatatg tgtagtgctt cttgaagttt gttatacttt aattaaaaga
     6481 gaggagtgat cgaatgatta caacaacaac gaacagtgta gagggaaaag ccgtagtagc
     6541 ttataaaggg atcgtttttg gtgaggtcat tactgggatc catgcattta aagacttgga
     6601 ggcggggttg cgaaatatct ttggcggccg ctcaaaaagc tatgaaaacg aactaatggg
     6661 tgcaagagaa gaagcacttg ccgaaatggc cgaacgtgca aaagcattag gagctgatgc
     6721 gatcattggc gtaaaaatgg actatgaagt attaggcagc gacaatggca tgctgatggt
     6781 cacatgtagt ggaacagctg tctccacgag ataagtaggg gacatctgtt ttaa
//
LOCUS       DAXLPI010000120         6725 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXLPI010000120 DAXLPI010000000
VERSION     DAXLPI010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.122"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            38..126
                     /locus_tag="ACF3T5_002298"
     tRNA            38..126
                     /locus_tag="ACF3T5_002298"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            141..212
                     /locus_tag="ACF3T5_002299"
     tRNA            141..212
                     /locus_tag="ACF3T5_002299"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:174..176,aa:Glu,seq:ttc)
     gene            228..300
                     /locus_tag="ACF3T5_002300"
     tRNA            228..300
                     /locus_tag="ACF3T5_002300"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:261..263,aa:Val,seq:tac)
     gene            310..382
                     /locus_tag="ACF3T5_002301"
     tRNA            310..382
                     /locus_tag="ACF3T5_002301"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Asp,seq:gtc)
     gene            418..493
                     /locus_tag="ACF3T5_002302"
     tRNA            418..493
                     /locus_tag="ACF3T5_002302"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Phe,seq:gaa)
     gene            495..575
                     /locus_tag="ACF3T5_002303"
     tRNA            495..575
                     /locus_tag="ACF3T5_002303"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Tyr,seq:gta)
     gene            582..655
                     /locus_tag="ACF3T5_002304"
     tRNA            582..655
                     /locus_tag="ACF3T5_002304"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Trp,seq:cca)
     gene            658..730
                     /locus_tag="ACF3T5_002305"
     tRNA            658..730
                     /locus_tag="ACF3T5_002305"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:His,seq:gtg)
     gene            742..813
                     /locus_tag="ACF3T5_002306"
     tRNA            742..813
                     /locus_tag="ACF3T5_002306"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Gln,seq:ttg)
     gene            859..929
                     /locus_tag="ACF3T5_002307"
     tRNA            859..929
                     /locus_tag="ACF3T5_002307"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:891..893,aa:Cys,seq:gca)
     gene            950..1034
                     /locus_tag="ACF3T5_002308"
     tRNA            950..1034
                     /locus_tag="ACF3T5_002308"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:984..986,aa:Leu,seq:caa)
     gene            1096..1168
                     /locus_tag="ACF3T5_002309"
     tRNA            1096..1168
                     /locus_tag="ACF3T5_002309"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1129..1131,aa:His,seq:gtg)
     gene            1178..1249
                     /locus_tag="ACF3T5_002310"
     tRNA            1178..1249
                     /locus_tag="ACF3T5_002310"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1210..1212,aa:Gln,seq:ttg)
     gene            1267..1351
                     /locus_tag="ACF3T5_002311"
     tRNA            1267..1351
                     /locus_tag="ACF3T5_002311"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Leu,seq:caa)
     gene            1606..2481
                     /locus_tag="ACF3T5_002312"
     CDS             1606..2481
                     /locus_tag="ACF3T5_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFD3632851.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2585..3349
                     /locus_tag="ACF3T5_002313"
     CDS             2585..3349
                     /locus_tag="ACF3T5_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFD3632852.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3336..4778
                     /locus_tag="ACF3T5_002314"
     CDS             3336..4778
                     /locus_tag="ACF3T5_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFD3632853.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            4789..6119
                     /locus_tag="ACF3T5_002315"
                     /pseudo
     CDS             4789..6119
                     /locus_tag="ACF3T5_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            6132..6590
                     /locus_tag="ACF3T5_002316"
     CDS             6132..6590
                     /locus_tag="ACF3T5_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFD3632854.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
BASE COUNT         2119 a         1093 c         1480 g         2033 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg caaactgtct caagggttcg aatcccttac
      121 tctccgtagc aaatttacct ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
      181 cggtaacacg ggttcgaatc ccgtacgggt cattttaatt gaatgttgga ggtttagctc
      241 agctgggaga gcatctgcct tacaagcaga gggtcagcgg ttcgatcccg ttaacctcca
      301 taatgaactg gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg
      361 ggttcgattc ccgtcggaac cgtcgttctt gttattcaat taataatttt aaaatatggc
      421 gcggtagctc agttggtaga gcaacggatt gaagctccgt gtgtcggcag ttcgattctg
      481 tcccgcgcca ccatggagga gtagcgaagt ggctaaacgc gacggactgt aaatccgttc
      541 cttagggttc agtggttcga atccactctc ctccatttta taggggcata gtttaaaggt
      601 agaacagcgg tctccaaaac cgttagtgtg ggttcaattc ctgctgcccc tgccatggcg
      661 aatgtggcga agtggttaac gcaccggatt gtggctccgg cattcgtggg ttcgattccc
      721 atcattcgcc ctttttattc ttggggtata gccaagcggt aaggcaaggg actttgactc
      781 cctcatgcgt tggttcgaat ccagctaccc cagtttctat aatttattct ttattttgta
      841 gattgataat agtataatgg cggtatagcc aagtggtaag gcagaggtct gcaaaacctt
      901 catcaccggt tcaaatccgg ttaccgcctt agtactaata aatcaacttg ccggcgtggc
      961 ggaattggca gacgcgctgg actcaaaatc cagtgcccgt tgagggcgtg ccggttcgac
     1021 cccggccgcc ggtattgact aagatgtctt catcttagtt ttttattgag aaattaaata
     1081 acatctttta tgatggcgaa tgtggcgaag tggttaacgc accggattgt ggctccggca
     1141 ttcgtgggtt cgattcccat cattcgccct ttattcttgg ggtatagcca agcggtaagg
     1201 caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt tattattttt
     1261 aaatttgccg gcgtggcgga attggcagac gcgctggact caaaatccag tgtccgttga
     1321 ggacgtgccg gttcgacccc ggccgccggt ataattatca atatagcaac tttcttatag
     1381 agagttgcta tatttttttt gtttttatgt aattacctcc aaatcaattc gatacaagta
     1441 ttatcataaa acgagtaaaa aagatattga taacgctttt attatgtgtt aaactatttt
     1501 tgtaaattat gtagggtgct actattcaat taggcacgac ctaagaaagg gttctttctt
     1561 aggtcgtttt ttgtctttaa aaattgtttg gagacaaaaa tatgtatgaa ggagtctgca
     1621 agttgttttg ttttatcata tagtggaggt gaattaacgg tgtatgtgat gaaaaagctg
     1681 aatcataatg ctgtattgat cgatgacgat ggaatagaaa aaattgcgat ggggaaaggg
     1741 attggctttt ctgctaaagt gagtaagcca tttgatgttg aggatgctga caaattattt
     1801 gtattagatt ctaaagaaaa gatcaaacga ttcagtgaga tagtctcgca gattccaatc
     1861 gaatttatgg attttgcgga agaagtgatt gaactgataa cagctaggat ctcctctcca
     1921 ttagattcaa atatctatat tacattgaca gatcatattt attttgctat tcaacgaaaa
     1981 agagaagata gagatgtcac agcaattatg ttgccagaaa tgagattcct ttatccaaat
     2041 gaatatgata tagcaataga agtggtcgag cgaatcaatc atcgatatca aaccgagtta
     2101 gggtcaaatg aagtaggatt catcactatg catatcatta atgctgagct aggagagcct
     2161 aatagtttaa agagcttaaa aatagtagaa atgacgacca cgattttgtc ccatctagaa
     2221 aaatcctatt caaacgttta tgataaagaa tcactgactt atcatcgctt aatggttcat
     2281 atcaaattcc tggttaaacg cttgatttat caggaagaaa cgccagtaga aaatattggt
     2341 ttttttactg aaacatttaa acatagcaaa gcgtattgta cagcactaaa gattaaagat
     2401 atcatcgaac agcaatttca acttgccatt caagaaaatg aaatcatcta tttggcaatt
     2461 catctagcac gaatctatta aaataagggc gtaactatct ataggcgcaa ccaaatgaca
     2521 catagtaaat ggactatgat tatttggttg cgcctatttt agtagaaaaa agaaggagaa
     2581 tgaaatggga aaagtgattt ttaattcgga tgattttggc tatagccatg gtgtgaatta
     2641 tgggattatg gatgcttatc agcgaggaat tttgacatca actacattga tggcaaatat
     2701 gccgggtttc gagcacgctg tgaaactaag aaaggaaatg cctaggctag gtgtaggagt
     2761 ccatttaaca ttgacgtgtg gcaaaccact tctcaaaact gtcgatactc ttatcgaaag
     2821 cggagagttc agatccttat cttattatca gcatccattt aacattgaca aagatcaact
     2881 gtatcaggaa tggaatgctc agattcaaaa aatttatcat gcggggatca taccaactca
     2941 tttagacagt catcatcata ctcatacttt ggatgaaaac caggaggtag ttgttacatt
     3001 agcaaaaaaa tatgatttgc cggttcggag caactttgaa cgaaaagatg aagttcgtca
     3061 tgtcgattat tttgagcctt gtttcgatgt cgtaggagaa actgatgaaa aaaaacgaca
     3121 aatcaatacg agtgtggaag aatatttggc aagtttactc atgatattga gagaagataa
     3181 aacgatcgaa gtcatgtgtc acgcagcgta tttggatcag gaattgttaa atggttcaag
     3241 ttttgtgtat cctcgtactc accaagtcga atttttaatt cattcagcat ttgctcaaaa
     3301 agtgaaaaat gatcctttga ttgagttagt aacatatgag gatatttaat agaggaggaa
     3361 taaaaatgaa tgttaaatca aatgtgcaga agcttggaag agcgatgcta cttccagttg
     3421 cagcgatgcc attagcggga ttgatcatgc gtttatcagc tgatgatatg ctgaacatcc
     3481 cagtaattgg tgcagctgga aatgccgttt ttggaaactt ggatatctta tttgctatag
     3541 gagttgcaat cggttttgtc aaaaataagg acaaaggaat acctgcactt actggttttt
     3601 tagcgattgc taccttgaaa aaaggcttag aaatcatgga cccagaagtg aatatgggga
     3661 tttttggtgg aattatttcc ggtttagtag cggcatggac gtataatcgc tttaaagaac
     3721 aaaaattacc agtagttttt tcttattttg cgggcgaaaa attcccattg actatggtga
     3781 tgattctaca aacaatcgca gctatttttt tgggctttat ctggccgctc atccaaattg
     3841 ggattgataa ttttgcaaaa ctgctcgttg atatgggggc attaggtgtg gggatattta
     3901 tgtttttgaa tcgtttgctg atcccctttg gactgcacca tgtgttgaat acgtatattt
     3961 attacgattt aggaagctat acatctccta gcaaggaagt tttcagagga gagatgacac
     4021 gctttatcaa cggagatcct caggctggac tgtttttatc aggttttttt gtagtaatga
     4081 tgtttggtat accagctata tgtctagcta tctataaagc agcttataaa gaaaataaag
     4141 agatggtcaa aggaatcatg ggaagcggtg caggaacttc atttattgca aatatcactg
     4201 agcctgttga atttagtttc atgtttcttt ctcctatgtt gtacgtgatc catgcacttt
     4261 ttgccggttt agcagggatt gtctgttact tattcaatat acgaattggt tttacattcg
     4321 gagcatgtat tgttgattat ctcatcaatt ttcgaatcgc taccaatgct attttgattc
     4381 ttccaatcgg aattttcttt tttgccttgt attacgttac tttttattat ttgatcaata
     4441 agagaaacat tcagacatta ggacgtgaag ctaaggcaga gtttggcaat gaagtaacct
     4501 tagaagaaac agagttaggg ttagcaagta aaaattatga atatatggca acaaaaatgc
     4561 tacaagcgtt tggaggaaaa gccaatatcc tggatgtcta tagttgtaat acacgcctac
     4621 gggtagaggt agtggatccc accatggtcg aggaacagcg aatcaagcaa ttagggataa
     4681 gcgggatcat taaaccaact gaaaaaaatt atcaaattat catcggctta gaagtgactt
     4741 atgttatggc agaattcaat aaattattag aagaatagaa aggaaaagat gatgatgaaa
     4801 aaacagatta tcactatagc tggaggcggg agtacgtata caccaggaat cgttcaagcc
     4861 attttgaaca atgaaaaacg gttgccatta tctgaaatac gtttatatga tattgatgaa
     4921 gaaagaaata tggatatgta cttgattgtt aaatttatgc tgaaaaaaaa ggtttttcaa
     4981 atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc gattttgttt
     5041 tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag attccactta
     5101 aacatggatt agtaggacag gaaacgtgtg gactcggagg atttgcttat gggatgcgct
     5161 caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc aaagcatgga
     5221 ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga
     5281 atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg attgcagtga
     5341 attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac cactttggct
     5401 ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag attattgaaa
     5461 aactaataac aaaagaaatg caagtcgccg attttaatat aggagacaaa acttggcaaa
     5521 aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc ccgaataatt
     5581 acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa gagtatacac
     5641 gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct gataaaatac
     5701 gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga caatatatcg
     5761 tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta atcgttccta
     5821 atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg gcatatgttg
     5881 gtgcaactgg tgtggagaca attacactaa gaaaaccaat cagtgatttt cataaaggat
     5941 tgatggaggc acaagttgca gcagagaagt tactagtaga tgcttacttt gaaggttctt
     6001 atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc agagtcgcaa
     6061 aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca ttaaagtaga
     6121 ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta gctagtggtc
     6181 aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg ataggagttg
     6241 gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga ataatcgata
     6301 atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga aaaatactga
     6361 tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata cgggttgaaa
     6421 gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag aagttggagc
     6481 aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa ggaaatttat
     6541 taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag tgtacgatga
     6601 aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg aaatgataag
     6661 tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt tttgttgctc
     6721 ctatg
//
LOCUS       DAXLPI010000121         6650 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXLPI010000121 DAXLPI010000000
VERSION     DAXLPI010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.123"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            115..1140
                     /locus_tag="ACF3T5_002317"
     CDS             115..1140
                     /locus_tag="ACF3T5_002317"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFD3632855.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            1315..2559
                     /gene="glyA"
                     /locus_tag="ACF3T5_002318"
     CDS             1315..2559
                     /gene="glyA"
                     /locus_tag="ACF3T5_002318"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFD3632856.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            2712..3659
                     /locus_tag="ACF3T5_002319"
     CDS             2712..3659
                     /locus_tag="ACF3T5_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFD3632857.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            3793..4422
                     /gene="upp"
                     /locus_tag="ACF3T5_002320"
     CDS             3793..4422
                     /gene="upp"
                     /locus_tag="ACF3T5_002320"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFD3632858.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(4561..4803)
                     /locus_tag="ACF3T5_002321"
     CDS             complement(4561..4803)
                     /locus_tag="ACF3T5_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632859.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            4924..4996
                     /locus_tag="ACF3T5_002322"
     tRNA            4924..4996
                     /locus_tag="ACF3T5_002322"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Thr,seq:cgt)
     gene            complement(5115..5753)
                     /locus_tag="ACF3T5_002323"
     CDS             complement(5115..5753)
                     /locus_tag="ACF3T5_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFD3632860.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(5876..6508)
                     /locus_tag="ACF3T5_002324"
     CDS             complement(5876..6508)
                     /locus_tag="ACF3T5_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFD3632861.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         2244 a         1163 c         1335 g         1908 t
ORIGIN      
        1 gtttatcaac aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt
       61 gaggataaac tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa
      121 acgaaacgat atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa
      181 cttgttgctt ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac
      241 gctgtcaaga aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat
      301 gtcgcctctt ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag
      361 attgtgaaga acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact
      421 ttgccctcag ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa
      481 aaaacactag aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg
      541 tcgggaaaac caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt
      601 accgggatta ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta
      661 agtgatccaa cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc
      721 gaagcagtca tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca
      781 ccaaaagccc ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc
      841 agagatggcg attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt
      901 gtcatagcaa gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg
      961 tataacgaca actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta
     1021 gatgaaccaa cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg
     1081 gggaatgctt acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga
     1141 tcaattggtt attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat
     1201 cagtgaacga aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat
     1261 ttgtttttga tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta
     1321 gattacaaaa cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa
     1381 gaacataatt tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca
     1441 caaggaagta tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt
     1501 gggtgtgaat ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc
     1561 ggagcgaaat ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat
     1621 ctcgctttgg tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat
     1681 ctaacacatg gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt
     1741 gttgatccga caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat
     1801 caaccaaaat tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa
     1861 ttcagagaaa tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca
     1921 ggattagtag cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact
     1981 accactcata aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca
     2041 ttagcaaaaa aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat
     2101 gtgattgcgg gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat
     2161 agtgaacaaa tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga
     2221 accagagtca tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta
     2281 ggtataaatg gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa
     2341 aactcgattc cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg
     2401 ccagcaatca ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt
     2461 gtcaaagcat tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta
     2521 cgcgaattaa ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa
     2581 acagaaaagt tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca
     2641 atatcggtga tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg
     2701 gaggaaaagt catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg
     2761 caaatgcgcc tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag
     2821 aagaagtaca agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg
     2881 atactggcga atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata
     2941 aattaccagt tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc
     3001 atgataaatt gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat
     3061 atagtcgatc accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc
     3121 aacagctgaa taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag
     3181 gagattcagt aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg
     3241 gcctcccaat atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag
     3301 attcctatca tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt
     3361 gggaccaata tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag
     3421 caaatataaa tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg
     3481 tattaagaga tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta
     3541 cacaagtacg cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga
     3601 caaaggcaac gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag
     3661 ctttgcttgt ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga
     3721 gtttgactag tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg
     3781 agagcttaaa aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta
     3841 actattattc gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc
     3901 gctatgttga tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa
     3961 acaccgatca ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca
     4021 atcttgcgcg caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa
     4081 gtgggacata tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc
     4141 aaacttccag aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca
     4201 ggcggatcag caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa
     4261 tttgtttgtt tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat
     4321 attgacatct atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct
     4381 ggactagggg atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt
     4441 gtgacacaac ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct
     4501 ttttcacagc tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat
     4561 tcaaatcttt tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa
     4621 gttcgcattt tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga
     4681 aatcgttcca gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa
     4741 tttttcacca atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt
     4801 cataaaacct gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa
     4861 actaattttc catcggactc tttacaatca gccaaataga gtatataatt tcttttggat
     4921 aaggccagct tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg
     4981 atccctatag ctggcattaa aagagaagac agatatatca aggattttta atccattgat
     5041 acatctgtct ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt
     5101 atttctataa aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc
     5161 acatgaactg cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac
     5221 aatcttgcca ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta
     5281 tcataagtac tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga
     5341 atgctgaact tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt
     5401 atcatgatag gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct
     5461 gttaaacccc atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca
     5521 ttgataaaaa aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt
     5581 ttcgtatctt ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa
     5641 aaaatgctaa taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata
     5701 gtgcccaata tccctttttt aagttctatt ttttttccta aaatgttatt catgataata
     5761 actcctacta ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa
     5821 tgttttttta tggatatttg tattgctaaa aatttatctt atatattcat tttgattata
     5881 ctacttttat ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac
     5941 aattttttag atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga
     6001 tattttttaa atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta
     6061 ttttagtgag ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac
     6121 tttcttttat gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg
     6181 tgttcccaat ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca
     6241 cgcgatgcaa catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac
     6301 agtcggtaat ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag
     6361 atttgtaaat aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga
     6421 tattatacag ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc
     6481 ttgcttcatt aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat
     6541 taacaaaagc taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga
     6601 taacgatagt gtcgctatct cttgaaacgt tcttagaatt gccctgagag
//
LOCUS       DAXLPI010000122         6563 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXLPI010000122 DAXLPI010000000
VERSION     DAXLPI010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.124"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(1227..1892)
                     /locus_tag="ACF3T5_002325"
     CDS             complement(1227..1892)
                     /locus_tag="ACF3T5_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632862.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2018..4054
                     /locus_tag="ACF3T5_002326"
     CDS             2018..4054
                     /locus_tag="ACF3T5_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFD3632863.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4058..4696
                     /locus_tag="ACF3T5_002327"
     CDS             4058..4696
                     /locus_tag="ACF3T5_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632864.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4724..5152
                     /locus_tag="ACF3T5_002328"
     CDS             4724..5152
                     /locus_tag="ACF3T5_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632865.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5609..6520
                     /locus_tag="ACF3T5_002329"
     CDS             5609..6520
                     /locus_tag="ACF3T5_002329"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFD3632866.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2262 a          992 c         1210 g         2099 t
ORIGIN      
        1 ttagtcctct tttttgacca gattttgacc agtctatttt acattctaat atattcatat
       61 gagctaattt tatgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
      121 gtaattcaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
      181 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
      241 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
      301 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
      361 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
      421 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
      481 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
      541 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
      601 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
      661 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
      721 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
      781 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
      841 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
      901 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
      961 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     1021 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     1081 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     1141 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     1201 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     1261 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     1321 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     1381 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     1441 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     1501 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     1561 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     1621 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     1681 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     1741 tgtaaaagtg acagcatttc aaagtctggc attgtacgag aattttccca actagaaatc
     1801 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     1861 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     1921 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     1981 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     2041 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     2101 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     2161 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     2221 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     2281 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     2341 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     2401 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     2461 agaaaaagaa gcctatgaac gggtcatttc actattcact gatcgattca atcaagcatt
     2521 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     2581 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     2641 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     2701 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     2761 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     2821 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     2881 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     2941 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     3001 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     3061 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     3121 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     3181 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     3241 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     3301 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     3361 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     3421 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     3481 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     3541 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     3601 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     3661 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     3721 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     3781 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     3841 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     3901 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     3961 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     4021 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     4081 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     4141 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     4201 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     4261 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     4321 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     4381 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     4441 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     4501 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     4561 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     4621 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     4681 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     4741 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     4801 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     4861 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     4921 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     4981 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     5041 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     5101 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     5161 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     5221 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     5281 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     5341 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     5401 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     5461 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     5521 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     5581 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     5641 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     5701 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     5761 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     5821 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     5881 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     5941 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     6001 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     6061 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     6121 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     6181 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     6241 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     6301 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     6361 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     6421 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     6481 tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa taaaaaatat
     6541 gaactattta aatcaataaa act
//
LOCUS       DAXLPI010000123         6552 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXLPI010000123 DAXLPI010000000
VERSION     DAXLPI010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.125"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            30..2627
                     /gene="adhE"
                     /locus_tag="ACF3T5_002330"
                     /gene_synonym="adhC"
     CDS             30..2627
                     /gene="adhE"
                     /locus_tag="ACF3T5_002330"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFD3632867.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2856..3962
                     /locus_tag="ACF3T5_002331"
     CDS             2856..3962
                     /locus_tag="ACF3T5_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFD3632868.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            3979..5003
                     /locus_tag="ACF3T5_002332"
                     /pseudo
     CDS             3979..5003
                     /locus_tag="ACF3T5_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            5009..5488
                     /locus_tag="ACF3T5_002333"
     CDS             5009..5488
                     /locus_tag="ACF3T5_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFD3632869.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5504..6058
                     /locus_tag="ACF3T5_002334"
                     /pseudo
     CDS             5504..6058
                     /locus_tag="ACF3T5_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            6114..>6552
                     /locus_tag="ACF3T5_002335"
     CDS             6114..>6552
                     /locus_tag="ACF3T5_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3632870.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENA"
BASE COUNT         2136 a         1140 c         1466 g         1810 t
ORIGIN      
        1 aataaccatg acccattagg aggataatga tggctaaagt aatagaaaaa gaaaaaacga
       61 agacaattga cgtacaagca atgattgatg atttggcaga aaaagcaaat gcagcattga
      121 aagaaatgga aagctttgat caagaaaaag tagaccatat cgtgcatgaa atggcaatgg
      181 cagctttgga tcaacatatg ccattagcga agatggctgt agaggaaaca ggccgcggtg
      241 tttacgaaga taaagcaatc aaaaatatgt atgcgtccga atatatttgg cataatatca
      301 aacatgataa aacagttggt gtgatcaatg aagatgtcca aaaagggttg attgaaattg
      361 ctgaaccagt cggtgtggtt tgcggagtaa ctccaacaac gaatccgact tccacaacga
      421 ttttcaaatc aatgatcgcg ttgaaaacaa gaaatccaat cgtttttgct ttccatccaa
      481 gtgcccaaaa atcttctgca gaagcagcac gagtggtacg tgatgctgct atcgctgccg
      541 gagctcctga aaattgtatc caatggatcg aacatccttc gatcgaagcg acttctatgc
      601 taatgaacca tccaggtatc gcgatcgtct tagcgacagg tggagctgga atggtcaagt
      661 ctgcttactc tacaggaaaa ccagctttag gtgtaggtcc aggtaacgta ccagcttata
      721 ttgaaaaaac agcaaaaatt aaacgtgcag tgaacgattt gatcgtttcg aaaacatttg
      781 ataatggaat gatttgtgct tctgaacaag ctgtgatcgt ggataaagaa atctatgcag
      841 cagtaaaagc agaattccaa gcacaccaag tttatatcgt aaaaccagat gaattgcaaa
      901 aattagaaga tgcagtgatg aacgaaggaa aatatgcggt caatccatca atcgttggac
      961 attctgcgat ggaaatcgca aaattggcag gaatcagtgt accaaaagga acgaaaatgc
     1021 tgattgctga attggaaggc gtaggcccag attatccgct atcaagagaa aaactttctc
     1081 cagttcttgc catgatcaaa gctaacaaca cggaccatgc gtttgatttg tgtgaaggta
     1141 tgttgaatct tggaggatta ggacatacag cagttatcca ttcagaaaac gaagagttgc
     1201 atgtcaaatt cggtcttcga atgaaagctt gtcgtatttt agtgaataca ccatctgcag
     1261 aaggcggcat cggtgatatc tataacgaaa tgattccgtc attgactttg ggatgcggtt
     1321 cttatggtaa gaactctgtt tctcgtaacg tctctgctgt aaacttaatc aatgtcaaaa
     1381 ctgtagcgaa acggagaaat aacatgcaat ggttcaaatt accgccaaaa atcttctttg
     1441 aaaagaactc tctattgtac ttagagaaaa tggaaaacgt tgaacgtgtc atgatcgttt
     1501 gtgaccctgg tatggtccaa ttcggttact gcgatacagt tcgtgaagta ttggcacgac
     1561 gtaaaaatga tgtgaagatc gaagtcttct cagatgttga accgaaccca tcgacaaata
     1621 ccgtatacgc aggaacgaaa ctgatggcag acttcaagcc agatacagta atcgctcttg
     1681 gaggcggttc tgcaatggac gcagctaagg gaatgtggat gttctatgaa cacccagaca
     1741 catctttctt tggtgcaaaa caaaaattct tggatatccg taaacggaca tataagatcg
     1801 acaaaccaga aaaaacacaa ttcgtatgta tcccgacaac atcagggact ggttctgaag
     1861 taacgccatt tgcggtcatc actgacagtg aaacacatgt gaaatatccg ttagcagatt
     1921 atgcattgac gccagatgtc gcaatcgtcg atcctcaatt cgtcatgtca gttccagcat
     1981 ctgtcacagc agataccggg atggatgtgt taacacatgc aatcgaatcc tatgtttcag
     2041 tcatggcttc agattataca cgtggattga gcctacaagc aatcaagtta gtctttgact
     2101 atttagaaaa atcagtaaaa acacctgata tggaatcacg tgaaaaaatg cacaacgctt
     2161 ctacaatggc tggtatggcg tttgctaacg cattcctagg tatttgtcac tcagtcgcac
     2221 ataaaatcgg cggggaatac gggatccctc atggacggac aaatgcgatt cttttaccgc
     2281 atattatccg ctataatgca aaagatccac aaaaacatgc gatgttccct aaatatgact
     2341 atttccgtgc agatacagat tacgcagata tcgcaaaatt cttaggtctg aaaggcgaaa
     2401 caacagagga attagtcgaa gctttagcaa cggctgttta tgaattagga aaatctgtcg
     2461 gcatcaacat gagtctaaaa gctcaaggag tcacacaaga aacactagat acaaccgtag
     2521 accgtatggc tgaattagct tacgaagacc aatgtacgac agctaatccg aaagaaccat
     2581 tgatctctga attgaaacag atcatcattg atgcttacaa cggataagca aaagaaagcg
     2641 tagaaaaaag ataagaaaat agatgaaaaa gaacttggaa aacgttgttt ctccaagttc
     2701 tttttttaat ttccaagtaa ctgaaagtta cttttgttat ttaacattta ggtcaatttt
     2761 agacagtttt tgttcaaaaa gcgatattta tagtggacgg tattactgct atattaagga
     2821 tataaaatga aagcgtttaa aaaggtggcg taaagatgat tattaaagaa gagatttttg
     2881 tacctaaaga attagtgaaa gaaaaaattg acctgctcgt tgaaaacctg acaacgatca
     2941 aagacgataa tggtgaattt ttgttggact ttgatggact gaaagtagat gacaaaagct
     3001 ggacagtgtg gaattggcca caaggtgtcg gtctatatgg tatttataaa aattaccaga
     3061 tgacaaaaaa tcctcgcgct tatcaagtgg tcaatgaatg gtttgaggat cgtatggaag
     3121 aaggcgctcc gcccaaaaac gtcaatacaa tggcaccgct attgactatg gcttacttgt
     3181 atgaagacac gaaagattca cgctatttgc cttatttaga acaatgggca gaatgggtca
     3241 tgaatgacat gcctcggaca aatgaagacg ggttacagca tgccacatat ggaccggaaa
     3301 ataagaatca gttgtgggac gatacattga tgatgacagt gcttcctttg gcaaaaatcg
     3361 ggaaactgtt gaatcgccca gaatatttag aagaagctcg ttaccagttt atgatccata
     3421 tcaaatactt gatggataaa cgaacaggat tgtggtatca cggctggacg tttgaaggaa
     3481 atcataacta tgccgaagca ttttgggctc gtggaaactg ctggttgacg attgctattc
     3541 cagagatcat tgaaatctta gaattatcga aagaagatgc attgcgcaaa ttattgatcg
     3601 aaacacttga agcacaagtg cgtgcattaa aaacttatca gtctgaaagc ggtttatggc
     3661 atacgttgtt ggatgatcct tcttcttacg tagaatcttc tgcaactgca ggatttgctt
     3721 acggtatttt gaaagcggtc cataaacgtt atttaccaca agaatataaa gaagtcgctt
     3781 atcgtgctat ccaaggattg cttgaacaaa tcgatgaaaa aggagaagtt caaaacgttt
     3841 caatcgggac aggaatgggg gacagtttag atttttatcg gaatatcgga atcacagcaa
     3901 tgccttatgg acagtcgtta acggtgcttt gtttgacaga attgctcgtc tcttattgtt
     3961 aaaagaaagg aaacccatat gaaaaaaaca tcactgctgt ttgcagcaag ttcgcttagc
     4021 ctaggtctgc taggtctaag cggctgcgga aataattcta gtgtggcaac tgtcgcagat
     4081 gcagacgtaa atgagatagt taccttgcgc tttgcttatg caagcaacag ccagtaattg
     4141 atgcaatgaa tgaatttggg cgtttagtaa aagaaaaaac agatggttcc gtacagatcc
     4201 agtatttccc agatggacaa ctgggtggag aacgtgaact gatcgaattg acacagtcag
     4261 gagcagtaga tttcacaaaa gtaagtgcct cagcgttgga aagtttctct aaagattatt
     4321 cgattttttc cgttccttat ctattcaatg atgaagaaca tttctttaag gtgatggata
     4381 atgaagaaat aatggagcca atctaccaat cgaccaaaaa tttaggattt accggattga
     4441 cctattatga ttctggtcag cgcagctttt atatggtaga cggtccgatc catacgccgg
     4501 aagatttgaa gggaaagaag atccgtgtga tgcaaagcga aaccgctatc aaaatggtag
     4561 agcttttagg cggttcgccc gtacctatgg gaagtgatga agtgtacacg tctcttcaat
     4621 ccaatttgat caacggatca gaaaacaatg aatttgtctt gtatacagct ggacatggcg
     4681 gggtagctaa atactattcc tatgatgagc atacacgtgt gccagatatc atcatcatga
     4741 atgatgccat caaagaacag ctaactgatg aacaggaaaa agccattgaa gaagcagcga
     4801 aagaatcaac ggtttttgaa attgaagctt ttgcaaaagc aattgaagaa gaaaaagctt
     4861 tagcacaaga agaacatgga gtccaattca atgatgtaga taatacaccg tttcgagagg
     4921 cagttgcccc acttcatgat atattcaaaa acgatcctac ctacaaagac ctttatcaaa
     4981 agatccaaga gcaaggagcg tagctgtaat gaaaatgatt ttaaacaagg tattagagat
     5041 cctgggagct gttttgcttg ttgggatggt cgctgttgtt ttatggcaag tattttccag
     5101 aacggtttta ggaaatccaa atacggtcac ggaagagctt gttcgattcg gtcttgtctg
     5161 gtttgccatg ttgtcttccg cgtatgttgt gggacaaaaa gcacgtttag ccgtaacggt
     5221 tttaagtgaa aaattaactg gcaaaaaaag taaaatttta gaagttgttg ttcaaagttt
     5281 atttctggta tttgctgcga tgatcatggt gtatggcgga tggaatgctg tcacattgac
     5341 aatggggcag atctcgcctt ctttaagtat tcctatggga tatgtttatc tgtcagtacc
     5401 tgtatcgggg gtactcatca ttatctatag cctgatcaat ctaatcgata cattaagcaa
     5461 caagccagca attcaagaaa aaatataaaa atggaggaat aaaatggcaa tagaagccgg
     5521 attagtgtta ttttttgtat ttgttgtgtt gcttgtatta gggatgccga ttgctataag
     5581 tattgcattc tcatcgatga tgactttatt gttagttata ccgtttgatg tttctgcttt
     5641 tagttcagct caaaaaatgg tgggaagtat caatagtttt tcgctgatcg ctattccgtt
     5701 tttcgttctc tcgggcatta tcatgaataa tggagggata gctaagaaac tagtggattt
     5761 tgcgatgttg ttcgtcgggc gcgtgccggg ggctttggct catacgaatg tgttagggaa
     5821 tgctttattt ggttccatct ctagctcggc cattgccgct tctaccgcga ttggtggagt
     5881 gttgatccct cagcaagtgg aagaaggata tgaccggaag tttgcgacag cggtcaatat
     5941 cgcgtcagct ccgacaggta tggtcattcc tccaagtact gcttttatca tgtattcact
     6001 tgttgctggt ggtgcttcga tctctgcttt gtttatggga ggttatctcg taggcgcttt
     6061 tgatgtgcac cccaaaagtt agactagaaa tctaattaaa aggggtgctt tttttgcgga
     6121 aatatacatt tgtttttaag aaaaaagtag tttcagacta tttaaataac gaaggcggct
     6181 acaaatatct tgcacataaa tatcaaataa atcgtacact ggttagacat tgggtaagga
     6241 tttataacta tcatggttgg gaaggcttgg ttggaggtgg caaaagctac actacaaaat
     6301 ttaaacttga tgttatagaa tatatggaaa caaatggtct ttctatccaa gaaactgcta
     6361 aaaaatttaa tatcggttca aatagaactc taagtaaatg gatagagcaa tatgaagaag
     6421 gcggtgcttc ttcacttgag agccaaaaaa ggggcagaaa aattagtatg aattccaagc
     6481 taaacattcc taaaaaactt aaagatgagt ctcttgaaga agaagttatt cgtttaagag
     6541 cagagaacgc at
//
LOCUS       DAXLPI010000124         6521 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXLPI010000124 DAXLPI010000000
VERSION     DAXLPI010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.126"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            292..891
                     /locus_tag="ACF3T5_002336"
     CDS             292..891
                     /locus_tag="ACF3T5_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3632871.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLSDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQVHDFSKYLFPSRKLADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            940..1410
                     /locus_tag="ACF3T5_002337"
                     /pseudo
     CDS             940..1410
                     /locus_tag="ACF3T5_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405176.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1624..1887
                     /locus_tag="ACF3T5_002338"
     CDS             1624..1887
                     /locus_tag="ACF3T5_002338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632872.1"
                     /translation="MKQIQNDLYGNIEKEYFDSQMFQQNSSPYYSEERFFQLMEQEEE
                     KRLLQLMKEMEEQQVAEEIEEKRFIKLIEEQMEIESLREQEEE"
     gene            2035..4137
                     /locus_tag="ACF3T5_002339"
     CDS             2035..4137
                     /locus_tag="ACF3T5_002339"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFD3632873.1"
                     /translation="MMTTQDLMDKYEVSRQTVNNWVRKNEIPAPTVKKGHQNAWTFSQ
                     AELIDKKLKHSHSEQLELFKTESTPLHINNRRYLGSKQKILDFINDVVSEHTKNVKSV
                     ADVFGGTGVVANLFNKQGKKVIVNDILTSNYISYETWFGNMPVDEKKIQFKIEELNSL
                     EGIHGYVDKNFGNKYFSVENAQKIDAVREKIETYNDLNEREKAFLLTSLLYAMDKVAN
                     TVGHFDAYRKKMDTFKPIYLRVPEFNKNVGNEIYNEDANQLVRKIKTDLVYIDTPYNS
                     RGYESAYHVLENVMEWKKPDVEGVAMKAVNRSEKSSDYTKSKAPQAFDDLIQNINARY
                     ILVSYNNMAKKGNSRSNAKISNEEIIATLKKRGQVEVFSIDFNAFTTGKSNIENHKEL
                     LYLCTVDDEIIQSPLNYTGGKQKLIPQLRPYFPSRYTRLIDLFSGGGSVTANLAKNGQ
                     AESYLMNDVENHVINFFEYISTIDIDDFINQAEMKISEYGLSNTKKNGYEFYSANSAS
                     GLGAYNKEKFLKLRKDYNNSPSPLLFYLLVVFGFNNQIRFNSKGEYNLPVGKRDFNKK
                     MEEKLRKFVKVIQDSPIEYSTKDFREVSIDTGDFIYADPPYLITTAAYNENGGWSEKD
                     EKDLYDYLDKANENNIKFALSNVIIHNGRENEILKSWASKYNLYVLNYNYNNSNYQSK
                     AKQSETVEVLVTNYERDI"
     gene            4137..6092
                     /locus_tag="ACF3T5_002340"
     CDS             4137..6092
                     /locus_tag="ACF3T5_002340"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HFD3632874.1"
                     /translation="MKLTNTAETFNLGDTSFRRKTLIDDYKTLLPYLQETNLEFNEWN
                     NVAQAEFYEKILTKTDLFSRNTDEDFAKRGRTLTNALVKIGLTNSKRKLSRVANSWIN
                     ENTLSANDVERSLGIDANNLLFTRQLLKLRVYDSNKMNYFYPFRVALGLVIKYQNIPQ
                     QDFLTLIHLIQPSFDNRKIKDIINDYHSVNDNNEIFSEFLDRNFPENTSELTADELFS
                     KKPLNRKEFDSLFVNRKSSATQGIYFEFVNKLLAFKSNKNVENLDALLELNSNDKLTK
                     AFGFGKAIFAKTNFMKSKNIQDFLDENVDNDLLSENNTLIYNQFVLSKKDDIVREYRD
                     MTKRTFNLSGLLDFNTGLVNATNQDVLSIIFSSMSFSGTENYADYEENLSYSFYQEST
                     ITEVLDLDESAILSEIKAFLGIENTSQIESVVLTQKEDKFRKFISKTFPREKIIEILP
                     LFSPRDDKKIKSEVSESATVPTIYEYIVAIAWFYISKEEFFITKSLNLTLDGEMRPLS
                     HAAGGAGDIVIDYENLTLMLEVTLMNAQAQKRGEWEPVLRHATNLTVDNFDKNVITLF
                     IADELDDNTVNIWRAVASVPLRSSNKDEIADLVKIFPLENRELLHMLKNNLDERKLLK
                     AIDESYEELAGNFDLSWRDKIFVEANM"
BASE COUNT         2377 a          937 c         1226 g         1981 t
ORIGIN      
        1 ttaattatat agactgaaaa ttttgaattt tcagaataga acaacttaaa agtactgaca
       61 aaatttttta aaggggcaat attaatgttc tgagataatc aaatcttacc gaaaatctca
      121 aaagtccaat gtgacacaag ctgattctgt cacattcgtt aaaatcgctc catccttaga
      181 ggctctaaga ggaaatcact tttcttttag ggcctttttt ctatagcttg tttaaatgtg
      241 acttattatc tgttataatc atattaagta acatttgaga ggggactaaa aatgaagagc
      301 gtggaaccga ttcgcgacaa aaaaaagatc gatgccatga aggccatttt ggcttccgga
      361 aaatatgggc aacgaaattt ggtgcttttt tcaatcggga tcaatacagc ctatcgaatc
      421 tccgatttaa ggcagttgaa attaagtgat gtgttagaaa tttcccgtgg gcgggtgatt
      481 gtcaaagaac gtctggccat gaaggaacaa aaaacggcca aacataattc ggtcttcatt
      541 tcaaacaaat tacgaaaagt gattctcgat tatgtccaat ccgaatttcc tgagcagctg
      601 caagtacatg acttcagcaa atatttgttt ccaagtcgaa aattagcaga tacgcctttg
      661 acccgacaaa gcctttggcg gatcattcat gaagcaggaa cagcggttgg tttaaaagaa
      721 attggtcccc attcgatgcg caagaccttc ggctattttt tgtacaagca agggaccaaa
      781 acagagatca ttcagtcgct gctcaaccat tcttcgcaac gggagacctt acgctatatt
      841 gggatcaccc aggaagataa agataccgca gtaaagagtt tggatctgtg acagaataat
      901 gccctatata atagaagaaa aaattcaaga ggggagaaga tgaacgaaga aaagcgtccg
      961 aaatttcctg ataaatacca cttatctcgc aaagagtctg tgtatttatt gaagaaaaac
     1021 attgtggaac tggtgtataa tgcgggaaaa tttgaagggt taaatacaac gctattacaa
     1081 acagaagaga ttatcaagta taatcgagct aataatatgg cggtagatga tgtcctgacc
     1141 gtcgttaact taaaaagagg ctttgaaatg cttttaaacg acttacagga gcccctaata
     1201 gagacaagta agcggattaa tcggatcgtt gcagcagagg atgcgctttt ttccggtgaa
     1261 atacgaactg gtggggtaga agtatcgacg attcaaggaa gatatgtgcc accaatgtta
     1321 actgaagacg aggtaaacaa tcaatatggt gagataatga ataaagagat atcagatacg
     1381 gaaaaagccc tgcgactgtt tctattcatt taatgcaata ggagcgggta tttgtaatgc
     1441 ttcccatagt gccaccccat ttttgaaatc aatttcccac gtttcttcgt tctcgccatt
     1501 ctttgggaga agctcaagag atggcggagt aaaatcaaat tcgcttttca ttatattaat
     1561 tgtctaactt aatttgacaa ttaatattat ttatacagca caagtgagaa atatattgaa
     1621 cagttgaaac aaatacaaaa cgatttgtac ggtaacattg agaaagaata ttttgatagt
     1681 caaatgttcc aacaaaattc tagcccctat tattctgaag aacgtttttt tcaattaatg
     1741 gaacaggagg aagaaaaaag actacttcaa ttaatgaaag aaatggaaga acaacaagta
     1801 gcggaagaaa tagaagagaa acggtttatt aaattaatag aagagcaaat ggaaatagaa
     1861 agtttaagag aacaagaaga agagtaagtt ctgcgtttgt tagaaacata atatctttga
     1921 aaacaattta gacaaacgac aggaaatggt aatttgaaaa ctaaacattg ataattactt
     1981 attgcactta atatggtaga attgcattaa ctgtaaagag agggtgatgc ctaaatgatg
     2041 acaacacaag atttaatgga caagtacgaa gtatcacgac aaactgtaaa caattgggtt
     2101 cggaaaaacg aaattcctgc acccacagta aaaaaagggc atcagaatgc ttggacattt
     2161 agtcaagcgg aacttattga caaaaaattg aagcatagtc acagcgaaca attggagttg
     2221 tttaaaactg aatcgactcc tcttcatatc aataatagaa gatatttagg cagtaaacag
     2281 aaaatactag attttatcaa tgatgttgtt tcggaacaca ccaaaaatgt taagtcagtt
     2341 gctgatgttt ttggtggaac aggtgttgtt gctaatttat ttaacaagca aggcaaaaaa
     2401 gtcatagtta acgatattct tacaagcaac tacatttctt atgaaacatg gttcgggaat
     2461 atgcccgtag atgaaaaaaa gattcagttc aaaattgaag aactgaattc tcttgaaggt
     2521 atacatggat atgttgataa aaattttggt aataaatatt tttcagttga aaacgcacaa
     2581 aaaattgatg cagtccgtga aaagatcgag acttacaacg atttaaacga acgtgagaaa
     2641 gcatttttgt taacgtcttt gctatatgct atggataagg tggctaacac agtaggacat
     2701 tttgatgcat atcggaaaaa aatggatacc tttaaaccta tttatttacg agttcccgaa
     2761 ttcaataaaa atgttggaaa tgaaatttac aatgaagatg ctaatcagct agttagaaaa
     2821 attaaaacag accttgttta cattgataca ccttataatt ctcggggcta tgaaagtgct
     2881 tatcatgttt tagaaaacgt tatggaatgg aaaaagccgg acgttgaggg agtggcaatg
     2941 aaagcggtca accgctctga aaaatcttcg gactatacta aatctaaagc accccaagca
     3001 tttgatgatt tgattcaaaa tattaacgct cgatatattt tagtttcgta caataatatg
     3061 gcgaaaaaag gaaattcacg ttcaaacgca aagatttcaa atgaagaaat tattgccaca
     3121 ttgaagaaac gtgggcaggt agaagttttt tcaattgatt ttaatgcttt tacaacgggc
     3181 aagtcgaata tagaaaatca taaagaatta ctgtatcttt gcacagttga tgatgaaata
     3241 attcaatcgc cactgaatta tacgggcggg aaacaaaaat tgattccaca attacgacca
     3301 tattttcctt caaggtatac acgattgatt gacttatttt ctggtggtgg tagcgttact
     3361 gcaaatctgg caaaaaatgg acaagcagaa agttatctta tgaatgatgt tgaaaatcat
     3421 gtgattaatt tttttgagta tatttcaact attgatatag atgatttcat taaccaagcg
     3481 gaaatgaaaa taagtgaata tgggttatct aatacgaaaa aaaatggata tgaattctat
     3541 tctgcgaata gtgctagtgg tttaggtgca tacaataaag aaaaatttct gaagctaagg
     3601 aaagattata ataattcacc gtcaccattg ctattttact tgttagttgt tttcggtttt
     3661 aataatcaga ttcgctttaa ttcaaagggt gaatataatc ttccagttgg taaacgagac
     3721 tttaacaaaa agatggagga aaaattacgt aagtttgtaa aagtaataca ggactcacca
     3781 attgaatatt ccaccaaaga tttccgagaa gtgagtatag acacaggaga tttcatctat
     3841 gctgatccgc cgtatcttat tacgacggca gcttacaatg aaaatggtgg ttggtctgaa
     3901 aaagatgaaa aagacttata cgattatcta gataaagcaa atgaaaataa tattaagttt
     3961 gctctttcaa atgtgataat tcataacgga agagaaaatg aaatccttaa aagttgggct
     4021 tcaaaatata atttgtacgt cttaaactat aattacaata atagcaacta tcagtccaag
     4081 gctaaacaaa gtgaaacagt tgaagtttta gtcacaaatt acgagaggga tatttaatga
     4141 agcttacaaa taccgcagaa acatttaatc taggtgacac aagttttaga agaaaaacat
     4201 taattgacga ctataaaact ttgttgcctt atcttcaaga aacaaatcta gaattcaatg
     4261 aatggaataa tgtcgcgcaa gcagaatttt acgaaaaaat tttgacaaaa acagatttat
     4321 tttctaggaa tactgatgag gattttgcaa aacgtgggag aaccttaacc aatgccttag
     4381 ttaaaattgg gctaacaaat tcaaaaagga aactttcaag agttgctaat agttggataa
     4441 atgaaaatac gcttagtgca aatgatgttg aacgttcact aggaattgat gcgaataatt
     4501 tattattcac tcgtcagctg ttaaaactac gtgtttatga ttcaaataag atgaattatt
     4561 tttatccatt tcgtgtcgca ttgggattgg tgataaagta tcaaaatatc ccacaacaag
     4621 attttctaac actaattcat ttaattcagc cgtcatttga taataggaaa ataaaagaca
     4681 tcattaacga ctatcacagt gttaatgata acaatgaaat tttttcggaa tttttggata
     4741 gaaattttcc agagaataca agtgaattga ctgctgatga acttttttca aaaaaaccat
     4801 taaatagaaa agaatttgat tcgctgttcg tgaatagaaa atcttcagct acgcaaggaa
     4861 tatattttga atttgtaaat aagttgttgg cgtttaaatc taacaaaaat gttgaaaatt
     4921 tagatgcttt gcttgaattg aattctaacg ataaattaac aaaggcattt ggatttggaa
     4981 aagctatttt tgctaaaacg aattttatga aatctaaaaa tatccaagat tttttagatg
     5041 aaaacgtaga taatgatttg ctgtcagaaa ataatacgtt gatttacaat caatttgtac
     5101 tttctaaaaa agatgatatc gttcgtgagt atcgagatat gacaaaacga acattcaatt
     5161 tatcgggatt attagacttc aatactggac tagtcaatgc taccaatcaa gatgttcttt
     5221 cgataatatt tagttctatg agtttctcgg gaactgaaaa ctatgctgat tatgaagaaa
     5281 acttgagtta ctcattctat caagaatcta caataactga ggtattggat ttagatgaat
     5341 ctgctatttt gtcggaaatt aaagcatttc ttggaattga aaatacttca caaatcgaaa
     5401 gtgttgtttt aacgcaaaaa gaagataaat ttagaaaatt tatctcaaaa acatttcctc
     5461 gagaaaaaat tattgaaatc ttaccgctat tttcaccgag agatgataaa aaaattaaat
     5521 cagaagtatc agaatcggca acggttccaa caatttatga atatattgtt gctattgcat
     5581 ggttttatat ttctaaagaa gaatttttta ttacaaagag tttgaattta accttagacg
     5641 gtgaaatgcg tccgttatca catgcagctg gtggtgctgg tgatattgtc attgattatg
     5701 aaaacttgac attaatgctt gaagttactt tgatgaatgc ccaagctcaa aaacgtggag
     5761 aatgggagcc agtattacga catgcaacaa atttgacggt agacaacttt gataagaatg
     5821 tcattacttt atttattgct gatgaactgg acgataatac tgtaaatatt tggcgagcag
     5881 ttgcaagtgt acctctacgg tcatcaaata aagatgaaat tgcagattta gtaaagattt
     5941 tccctctgga gaatcgagaa ctacttcata tgctgaaaaa caatctcgat gaacgaaaac
     6001 tattaaaagc aattgatgaa tcatacgaag aacttgcggg caattttgac ttaagttggc
     6061 gagataaaat ttttgttgaa gctaatatgt aaaaatgtag tagtttcgat ttaaacggga
     6121 atgtttttta gtcaatggaa aatatcactt aatggtgtga acttatggta aaagctagct
     6181 caattcgttt tatttagggt taaaattcac accattcaaa tgatattagt tgaaattaga
     6241 tgaagttttg ttatttgcaa tcgcccaaaa gatttgataa taaaagcttt tgaaagttat
     6301 tcaattaggc gttgttcaag ccaggtctta aaggcgctcg tcgttcacta cagttctcaa
     6361 cgttaatatc gttgctattt caacgtttac agcactcttt ggattatttc aaagagtgtt
     6421 tttttgtatt tttatagagg tatttagata agtttgtgga ggtttgttgc gctttatttt
     6481 gctatccagg agttcattat tttaaaagct taaataataa g
//
LOCUS       DAXLPI010000125         6217 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXLPI010000125 DAXLPI010000000
VERSION     DAXLPI010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.127"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            430..762
                     /locus_tag="ACF3T5_002341"
     CDS             430..762
                     /locus_tag="ACF3T5_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3632875.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            672..866
                     /locus_tag="ACF3T5_002342"
     CDS             672..866
                     /locus_tag="ACF3T5_002342"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFD3632876.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            905..1051
                     /locus_tag="ACF3T5_002343"
     CDS             905..1051
                     /locus_tag="ACF3T5_002343"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632877.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            1489..2829
                     /locus_tag="ACF3T5_002344"
                     /pseudo
     CDS             1489..2829
                     /locus_tag="ACF3T5_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3070..4686)
                     /locus_tag="ACF3T5_002345"
     CDS             complement(3070..4686)
                     /locus_tag="ACF3T5_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFD3632878.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4804..5022
                     /locus_tag="ACF3T5_002346"
     CDS             4804..5022
                     /locus_tag="ACF3T5_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632879.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5217..5678
                     /locus_tag="ACF3T5_002347"
     CDS             5217..5678
                     /locus_tag="ACF3T5_002347"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3632880.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5871..6110)
                     /locus_tag="ACF3T5_002348"
     CDS             complement(5871..6110)
                     /locus_tag="ACF3T5_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632881.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6134..>6217)
                     /locus_tag="ACF3T5_002349"
     CDS             complement(6134..>6217)
                     /locus_tag="ACF3T5_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFD3632882.1"
                     /translation="YNEMHNWPTFYPASLSEHEIAQEMMAH"
BASE COUNT         2063 a         1212 c         1045 g         1897 t
ORIGIN      
        1 taaagtatcg attttacagc gttctataat gattcataga atctaaaacg aatgatttta
       61 attatcaacg ttttttaatt cgtttttagc ttaaaaataa cttttgtcac agtctcactc
      121 ctaaaattac tttatttacg tatgaagtgg gcaggaggtc gaatccagaa ctgggctatg
      181 gtacttcaac gaggacttct cctctagaat tgaaaagcat gcgatttacc ttccgtaacg
      241 acggtagatt tattcaacta tgtgtccaat caaaatttgt ctgatttcaa aaatagtcaa
      301 gagaattttg gttgttaaaa actcttatct atcaacttcc acagtttagt tgacaaaccc
      361 tgatactaag ttgggctatg atacactttt cttgactatc agttacgtta ccggaaggaa
      421 tgaactctat tgagaaaaac atatgatcgt aaatttaaac taaagatttc acaggatatc
      481 cttgagaaaa aagtaactac caagaaaatt gctgaagaat acaatatctc tcgtccaaca
      541 atttcccgat gggtttcaga atatcgtcga tacgggaaaa atgcttttgc tggacaagga
      601 aaaagattac ctgataaagc agatttctac attcttgaac aggaaaataa acgactaaaa
      661 gaggaagacg agtgttctac gaacacaaag atcgatacgg atctccaggg atcactcaaa
      721 aactttacga tgaaggaatc gaaatcaata aacgtgttgt agctgttctt atgcgtgaaa
      781 tgaatttatg cacaagagga tttcatcgac gcaaatcttc tcatggtaga gaaaaaacaa
      841 tcaaagaaat ggtaaaaaaa tgttgaatcg ataatttgaa cagaaccaaa ttgatgctgt
      901 ttgggtgatg gatattactt acattccatg taatgacggt cgcctctatt tgtccaccta
      961 tattgactta gcaactcgta ttctacgtta ctatatggta gattcacaca tgaaaaaaga
     1021 gattgtcgtc cgtccattac aaatttatta aggagaattg cctacggtaa ttcattccga
     1081 tagaggtagc cagcatcggt cttttattta tcaagagctg ttgaacgacc accacatcac
     1141 acattcagtg agccaaactg ggactcctgt tgacaatgca gtaattgaag cgtttcatcg
     1201 atcaatcaaa tgagaattaa tcgactccaa caaatataaa agtaaaatag agatgaaggt
     1261 gttaatccaa gattatttag aagactacta cccaaacaaa agaatccata caaaatttat
     1321 gatgactcct tgacaatttg aagcgaagaa aataagggat ctataatatc tagtgttggt
     1381 gacgccttct gttgccaata ctttttcttt tttctaataa acgcaaacag acaccttaaa
     1441 atcctttaga ataaagtcga cgaaaaccat tcaaaggagt gtttcaagat gtcctattca
     1501 gagtttacca aagagttact agatattcta gacctaaatc ttatctttca tgaagatgcc
     1561 tttcgcaaag agcgaatcaa tgacgaaaca tgttttgttt ttgatggcac attgacctat
     1621 caaccggagg aatgctttca tcgccactat caaaatgaac aaacgatcat taaatagggc
     1681 tggaaaaaag tatccatctt attgaatgat gtcagtaact acaagaccat tctgcggatt
     1741 aacaaacagc gcttcaaatg taaacactgt ggaaagactt ttttagcaga agattcagtt
     1801 agtgatcgtc gctgctccat tgctcgcaga gtcaaacaag ccattcttga gctattaagc
     1861 gaacctcttt ctatgtcttt gatcgcacgt atgaagcaca tttctccgac cactgtcatt
     1921 cgaatacttc gcagccttcg gcctaaaacc atttctttga gccagctgtt accagaagtg
     1981 gtgtgtttcg atgaattcaa atcagtgaaa aatgtctcgg gagccatgag ctttgtcatg
     2041 atggacggca agacccatca actgattgat attgtggaaa atcggcaact caatccttta
     2101 cgcgactatt tcttgcgcta ccctataaaa gtccgtgaac aagtgcgtct agtggtttca
     2161 gatttctata caccctatcg cacattggtg aaagagtgtt ttccgaatgc ccgaatcgtc
     2221 gccgatcgct ttcatattag ccaacacatt ggtcgggctt ttaccaacca ccgaattcaa
     2281 gtaatgaaga cctttaaaaa aggggatcgt cggtcgaagc acttaaagaa atactggaaa
     2341 ctacttcaaa agaatgcgtg ggaattgaaa ggtcagcatc gctactggcg tccgtcattt
     2401 cgagatcatc tgacagaagc agagatagtc gatcggctat tatcttatga tgattcctta
     2461 aagcgaggct acgaagtcta ccaggatttt ctttccgtta tcagaagaca agatgttcct
     2521 gaattcgtcg cgctcctaaa agaagactac aaggagttgc cagagcacta ccaaccagtg
     2581 ttcacgacct tcaagaaata ccaaacagaa atcaaacgag cgttgcgcgt tccctattcc
     2641 aacggcccta tagaatgttt gaataaccat atcaaagtac taaaacgaat tgcctatggc
     2701 tatagaaact ttcaaaacta ccgagaacga atctttttat atcgaggaaa atattttaag
     2761 aaaactaaga atactaccca actgactaaa gccagaacaa caacacggct agacaagata
     2821 gctgtctagc cgtgtgagta tctcgattat tctattgaat ttcttcacca acactcattg
     2881 acgaagagcc aaaataagta atctaaccga ctgttaacta aaccattgca ggtcccgtta
     2941 aagatacttg tgcctgttgt caccgcttct ttttcttgtc cacaagcgct taacacactt
     3001 aataaagccc ctgctattat taataataat atctttttct tattaaaaac ctcctcatct
     3061 atttccagat tagtaaatgt agtaatctgt gctttctttt ttctcaatac taataactaa
     3121 cttatggggt aaacaaatac tcgtttgacc gggttttgag atccatccag ttctaaccgc
     3181 gatttgatca ggactattat cttctttaac tcgaattctt ccatctttaa tctcaacaat
     3241 attatattgt cccttcgcag gatgataatt cacgagttta tgattgatct tatccaaatc
     3301 gaagcgatcc atttccttgc catcaattcg aacgatggca tatttgatag catcttcagt
     3361 tgtttcattc tccttttcta tagagaatac aaataaggga ataaaagagg taataatcaa
     3421 aatgccaata attatgacat cgcctatctt gacaccagct tttcttaaaa atttcattaa
     3481 cgatatctgg ctaaacatat tagacttttt ccatggaaat ggtgaaattt ttcggaaatt
     3541 atctgcatta agagaatcag ttaaacaacc ctcaagttgt tccttaatac attgttgatc
     3601 catcttcata ccaataaaaa ctagtttatt aatgcgttca ccaaacaacg gatgccaggc
     3661 ttccttcaat tcgggatttt tttgtataat catttgacac tcaattttag gtaaactagc
     3721 tacccaataa tttaatgatt caatactttt atagctaccc gatgtttcaa agatcgtcga
     3781 aaaatcacta tgttgggcaa accaaagaaa cccttttgct cttgtaatat tgccaggaaa
     3841 atttttatcc gtaagatggg caaatttttg tgcgtcaagg agtaaccgat ttcgatagat
     3901 aaaacttgag ataccatatt cttctgtttc aggaatatga ttttgatagc caagctctaa
     3961 ctctttgaac cagcccactg aataggaagt ctcatctata ttaaaaagat ttgtatttag
     4021 aattttcgat aagtctactt cacctttaat tgtcctaatt attttggcat tggactgtaa
     4081 cttagcaatt agaccgttaa ttttgtaaat cgtcttggtt gacactaagt cactattatt
     4141 taaaagtaat atgttacaac actcgaattg atcaattaac aagccacgaa tatcctgttc
     4201 ttcttcaact acattacctt cagcttttct ttcctgaatc ttttcacccg tttcgaaatt
     4261 atcccaaaaa cgttttacat ccactactat aataatggca ccaatctcta atcgttgggt
     4321 caaatcaatg tctaaagggt ttttagctaa tataattgct tgaataattg gaattggttt
     4381 gctgattcct gaagcttcaa taacaatttg attcaagtca gaaaattgca ctaattgatc
     4441 tagttcaaca atcaaatctt cgcgtaatgt acaacaaatg catccattcg gcatcgaaac
     4501 aagtttttct ccagttcttt taaaaccttt cttgtcgact aataccccat caacatttac
     4561 ttcccctatg tcatttacaa tgacagcaat ttcctttccg tgatcaccgt acagaatatg
     4621 atttagaagt atcgtttttc ctgaaccaag atagccagtt aagattgtaa taggtatttt
     4681 tttcataggg aacctctttc tataccttga atttacactt taagtgcaac acataaaaat
     4741 gagtcataat cgatatccaa ctagtataag tttgtcgaga acactcctag aaggaaaata
     4801 attatgagcc agtatcgtca tcttactata ctcgaacgag aaagaatctt tctgcttcac
     4861 gaagaaggat tttctattag tataattggc aacgacattc agcatagccc gtttactatt
     4921 ttacatgaac tgagaagaaa caagaaaaaa actattcacc ttccaaagcg gaaaaaaatt
     4981 atgcttatcg aagaaaaaaa tgcggacgtt atcacattct aaatgatccg tgagtttggt
     5041 caattgttcg tcatttattt gtcgattttc agtggtcgcc agaggaaatc tcgaatcgtt
     5101 tgaagcatga acatatggac ttttcaatta gttttatcac gatctaacgt gcaatttgca
     5161 atgatctttt cgatacagaa cccctctcac acggcaataa aggcctgatc cgttcattgc
     5221 accatcgagg aaaaacaaga catacaaaaa atcatgtcga gtctcgtgga aagatttgcc
     5281 ttactcattc gatccatggc cgtccaaaat ccgcagatga tagaagtgat tttggtcatt
     5341 ggaaagccga tattgtagct ggcaaaactg gtggtacttg tttagtgact ttgacagaca
     5401 gaaaatcatg cttcctttta gcaggaaaga aggtccccag aaagaaagcc gcttatgtga
     5461 gcgagaaaat gattgaactt ttaagcagta ttccaaatga ggcggtcaaa acgatcactc
     5521 cagatagagg aaaagaattc tccaatcatt tcgcagttac agaagcattg aataatatgc
     5581 ccttttattt tccagatcct catgcccttg gcaacaatga acaaattaaa atacgaatag
     5641 actcattcga gaatacttgc caaaatcgaa agagatagct acggtagata actcaacgat
     5701 aaatcagatg attgaaaaac tgaataacag acctagaaag tgtttagatt ggagaacacc
     5761 ttatgaagtc ttctttgata aagtgttgcg cttgatttga caattcaaga cataataaaa
     5821 agcagatcaa atccaaataa gggaaaaatg atctactctg gtattatcaa ttaattggcc
     5881 aagctattat gccgttttgc taaacgtcta cttcttccag tattgacctt gataaaacaa
     5941 caatcaggat cccgttttaa tactctatga actgtgtcaa tactaacatg taaagttgca
     6001 gcaatatcag ctaatgtact cccatttaat ctcatttgct ttgcaaatcg ataacgccga
     6061 taagcatcaa tattcttgga atccatagac aataatactt ttcttaccat gtattttctc
     6121 caaagattgg attttaatgg gccatcattt cttgagcaat ctcatgctca gataatgaag
     6181 ctggatagaa agtaggccaa ttatgcattt cattata
//
LOCUS       DAXLPI010000126         6211 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXLPI010000126 DAXLPI010000000
VERSION     DAXLPI010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.128"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..164)
                     /locus_tag="ACF3T5_002350"
     CDS             complement(<1..164)
                     /locus_tag="ACF3T5_002350"
                     /inference="COORDINATES: protein motif:HMM:NF045046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HFD3632883.1"
                     /translation="MNILILSCGRRCELVNAFKTALNNNEMEGKIIGADCSELAPALY
                     MCDEKIVLPKV"
     gene            complement(176..1993)
                     /locus_tag="ACF3T5_002351"
     CDS             complement(176..1993)
                     /locus_tag="ACF3T5_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFD3632884.1"
                     /translation="MFVLTRRRKIATLVVVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFSSLSASAASSILILLFINRRYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYVSADSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVVGFVDDDPNKRNTYLSGKKVLGALKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESARVKVNTMPSIEELASGKISVSKLKTIDVVDLLGRDEVKLDIESI
                     KDQITDKVILVTGAGGSIGSEICRQIIQFNPAKLLLLGHGENSIYLIDRELRTHHQNC
                     PTEIVPIIADIQDREKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPITVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSIQKVMDFVKSLPQDDEQLAKDIVTFANASNR"
     gene            complement(2006..2770)
                     /locus_tag="ACF3T5_002352"
     CDS             complement(2006..2770)
                     /locus_tag="ACF3T5_002352"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFD3632885.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNAHFRKNPNYLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIGKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2798..3496)
                     /locus_tag="ACF3T5_002353"
     CDS             complement(2798..3496)
                     /locus_tag="ACF3T5_002353"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFD3632886.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLSNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQIFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLSKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3508..4287)
                     /locus_tag="ACF3T5_002354"
     CDS             complement(3508..4287)
                     /locus_tag="ACF3T5_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFD3632887.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKEAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNGKTAPSRRSRSRV"
     gene            complement(4303..5247)
                     /locus_tag="ACF3T5_002355"
     CDS             complement(4303..5247)
                     /locus_tag="ACF3T5_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFD3632888.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5293..6072)
                     /locus_tag="ACF3T5_002356"
     CDS             complement(5293..6072)
                     /locus_tag="ACF3T5_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632889.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTIVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIDQIRNEKIRAAYATKAQTVAQQMGYSNISY"
BASE COUNT         1622 a         1303 c         1125 g         2161 t
ORIGIN      
        1 actttaggta atactatttt ttcgtcacac atatatagtg cgggtgctag ttcagagcaa
       61 tctgctccaa tgatttttcc ttccatttca ttattgttta atgctgtttt aaatgcattc
      121 actagttcac aacgacgtcc acaacttaat attaaaatat tcaattttta atctcctatc
      181 tattcgaagc atttgcaaaa gtgacaatgt ctttagccag ttgttcatcg tcttgtggca
      241 ggctcttaac aaaatccatc actttttgta ttgagtaacc tttgatgttt cctacaaaga
      301 ttttctcata aactgcttca tcatttcttt ccttatccaa caatagttct tcatatagtt
      361 tttcccctgg acggatacct gtttcaatga tttcgatttc gtcttcgcta tacccgctca
      421 atcggatcat gtttttggct aggtctacaa tcttcactgg ttcactcata tctagaataa
      481 aaatttcgcc accttttgct aatgcgccag attgaataac taaacgactc gcttcaggaa
      541 tcgtcatgaa gtatcgggtc atacgaaagt ctgttacagt gattggacca ccttgagcaa
      601 tttgttctct gaatactgga atcacactac cgcgagaacc aaggacattc ccaaatctga
      661 cagctgagaa tttcgtacag ccttcttcat tcaatccagt cacgatcatt tcagcgattc
      721 gttttgttga acccatgacg tttggaggat tgttagcttt atctgtcgat accatgacga
      781 agtttttcac ttttgctgct ttagctgctt ctgctacatt ttttgtaccg aaaatattgt
      841 ttttgactgc ttcttttgga ttatattcca tcagtggtac atgcttatga gcagctgcat
      901 ggtagacgat atctggatga tattgttcca tgatctcatt gattttctcg cgatcttgga
      961 tatctgcgat gatcggtacg atttctgtag gacaattttg atgatgcgtg cgtaactcac
     1021 ggtcaatcaa gtagatcgag ttctccccat gtcctaaaag caatagcttt gcaggattga
     1081 attgaatgat ttgccgacag atttctgaac cgatcgatcc tcctgcgccg gtgacaagaa
     1141 tgactttgtc tgtgatctga tctttgatgg attcaatatc cagctttacc tcatctcttc
     1201 ccagtaaatc cactacgtca attgttttta attttgatac gctaattttt cctgaagcaa
     1261 gttcttctat agaaggcatc gtgttgactt tcacacgagc ggattcgact aactcgaaga
     1321 ttcttcgtaa tttttttctt gataaggaag ggatagcgat cgttaccatt tgaatgtcat
     1381 atttttcaat caattccgga atatctttta atgcacctaa tacttttttt cctgaaagat
     1441 acgtatttct tttatttgga tcatcatcta cgaacccgac aacgtggata tcttgagccg
     1501 ttttggatcc aagaaagctg ttgtataaaa tacgcccgcc ttcgccagca ccaacaatca
     1561 atgtattttt agcagaatct gctgatacat atctcatatt ttttgtttcg atgtagatac
     1621 gccagatcaa tcggctaccg atgatcaata agagcgaaag cagataagca aagataacta
     1681 accgtaaact gtatcgccga ttaatgaaca gcagtatgag gatacttgag gcagcacttg
     1741 cagatagtga actaaaaatt gcaatgatct ctcttaaatt tgtatagcga ttgattcgag
     1801 tgaaaacttt aaataaacta ccataaaata aataaaaacc aatcgacaaa ccaatagaga
     1861 ttaaaataag gtccattggt atagctacaa acggcttcat gaatttagct gctgcaatat
     1921 ttgcagcgat taataacaag ctgtccacaa caaccaatgt ggcaattttt cttcttcttg
     1981 ttaatacaaa caccctacat tccccctaaa ataaaccaaa tttcttcttt tttacctctt
     2041 catagactgg ataactgatt tcatccccgt tgatcagatc ctttgcgact tgtttcatct
     2101 gtactacttt ttccttacca aagtccttgc caatctgctc atagcactct tttaagtaaa
     2161 aggtgcgctt agagacccca tgggcatcag aagcagccat ctgcacgaga ttatgtttga
     2221 ccatcgtttt agctgttttc tggatgtctt tgccaaacac cccaacataa cttggcgcag
     2281 taagttgggc caaacacccc atatctagga acggaagcaa ataattcggg tttttacgaa
     2341 aatgcgcgtt acgttctgga tggacgatca ctggtgtctt tcctagttct aacaactcaa
     2401 aaaaaagccg ttctgcatat aacggcactt ccagtgtcgg aaattcgatc agaagatatg
     2461 tatcatctaa atctgtaaat aaaatttcat ctcggtggat gtcttcgatg agcgttccgg
     2521 taatacgtac ttcttgtcct tccaataacg tcaaaggcaa ttgtcttttc tctaattcgg
     2581 cttgtaacga agcgactaga gaaatgactt gtgatttttc attgctgtat tttccattat
     2641 tgtggtgtgg tgtgcataaa atatgcgtga tgccttgctg aatcgctttt tcagccattg
     2701 caatacttgc ttctaagttt tctgccccgt catcgattcc tggtaggata tggcaatgta
     2761 gatcgatcat ttctcttgtc tcctctcttc ttattcctta atttccgtag taataaccag
     2821 catctttgct atgttctgct ccgttgtata caacacctaa gactcgtgct tgtaccatct
     2881 ctagcagttc ttttgcttta gacaaggatt ctttacgtga tgtattttca cgtacgacca
     2941 ataaagtgcc atccgttttt gaagacatga tctgtgcatc tgttacggct actactggtg
     3001 gcatatcgaa tatcaccacg tcaaatatct gtcttgcttc ggctagaatc tgatccattc
     3061 ttggcgagct taacaattct gatgggttgg gtggcttagg cccactaggc aaaatcgaca
     3121 agttctcgac tggcgttcgt tggatcacat ctgctacact tcctgatgaa ctaagtgctg
     3181 tacttaatcc actagcattg ctcaactgga atgttttata gacgacgggt ttacgtaaat
     3241 ctgcatcgac taatagaacc cgttgaccgg atttagcaaa aacgactgca atgtttgctg
     3301 cagtggtgga ttttccttct ccaggacctg atgaagtcac gacaatcgtt ttgatttgct
     3361 gaccagctgc ggaggcaaac tggatattcg tccggatcgt acgatattgt tcggcgattg
     3421 gtgaagatgg gtttgttaat gtgatcaaac tgactgcatg agtttgtgct gctttttgtt
     3481 tctgtgttcg tgccattttt cttcctccta gactcttgag cggcttctgc gtgatggcgc
     3541 tgttttacca tttgtagctt ttggtcgtgc aggagctgat ccttttttac ggataccagc
     3601 gttcaattct ttcgctgaca tttctggaac tgttcctaag attgggaatc ctaatgtatc
     3661 cgtcacatat ttttcatctt tcactgtccg atcgagtaat tcgaataaga atgccagacc
     3721 tacgcctacc atcattccta atactaagcc aatcactaaa ttcaatttat tatttggcga
     3781 aacaggtgtc gtattggcaa ctgcacctga aataatcgag attttatcga cattcatcac
     3841 gtcttttgcg ttctcttgga aaacttgtga agttgtgtta gcgatctgtt gagcagtcac
     3901 tgcattcgtg cttattgcct ggatcgagaa catttgtgaa ttttgtgact gattgactga
     3961 gatggcctct ttgatttgtt cagcagtcat tttcacattg tcttctgttt ctaaacgatc
     4021 tttgacttca tttagcacta aatcactgac gatcatatct ttatacgtat tgatcatttg
     4081 caggttcgta ttgacatcat tgacatttgc tgtgtctgat tgtggcagtg tcacgatcaa
     4141 ttgtgcttgt gaactgtatt ttggtgtcaa gatgaaaaat gttacgattc ctgagatggc
     4201 caagccagca aacattgaga tgatgattgt taccaagtgt ttgcgtaatg tttcaaaaat
     4261 ttctttcaag ctgattgtct cttccatttt ttctccactt tcctatttat tctctagatt
     4321 catttgatct tttaattctt ttttcacacg atcaagttct tgatcgctga ctttttgata
     4381 ggagatatta tcttgcatga agccttctcc ttgcagctga tcttgcttga tcgtaccaaa
     4441 tgcctcccga taatctaaag caattttttt catttcatca aagctcatgt ctgttttcat
     4501 attcgactct agagcattca aaacggattg atagttgctg acaccgttta gactcaatac
     4561 ttttttcgct acaccttcta cgatctttct ttgacgctct tgtctaccat agtcaccatt
     4621 tggatcttca taacgcatac gagaataaga taatgcctga tccccgttca aagtaatttt
     4681 accgattgta aagtcatagc catcttgtga gaaagtgagg tcattattga cttcgatacc
     4741 accaactgca tcaacaagtt cttttagccc tttcatgttg atagagacat agtgatcgat
     4801 tgggatgtct acatattttt gtacggtatc catcgccatg gcagctccgc cgaatgcgta
     4861 agcgtggttg atcttgtctt ttgtgtcacg acctacgatg tcgacatacg tatctcgagg
     4921 aatgctgacg atcgttgtct gtttgtcttc aggactgact gtggcaacca tcatcgtatc
     4981 tgatcttcct tgatcgaccc gacctaaatc tccagtatca atgcccatca atagaacaga
     5041 gaaagattct tgtttgtcca gatcaactgg tttcgttcgt tctccttggg ttcgttccac
     5101 tgattcgtaa gttttctcaa tagaatgtga gagatccaga tataattttg ctcctactcc
     5161 tacaattgct aatactaata cgataagaac tgctaaaata atcagaaata tttttttccc
     5221 tcttgatttt cttcttcgtc ttgccatagt ttcctccgtt tgctaacctt gcttgaatca
     5281 acgttttaac tgtcaatagc ttatattgct atagcccatt tgctgagcaa cagtttgtgc
     5341 ttttgttgcg tatgctgcgc gtattttttc attccggatc tgatctattt gactgagcac
     5401 cgtcaaatat tgatcgtaag tagcggtggc agtcacttga ccatccttca acatcgtatc
     5461 gaataactga tctagttgcg tgacttgcgc gatctgagta tcagcgaatc ctagatattg
     5521 cataacaatc gttttccaag gactatcttt aaaaaagctc atattttcac gtacacgagc
     5581 gacatctgca cttgtcacct tttctttgat gacgacatcg tctacagctt gctgccagtt
     5641 tgatacattt ttagtaaata aatcgttgac agctgtctgg atcttcagtt ttgattctgc
     5701 actatctaac tgtgtcacga ttgctttctt ctcatcttgg attgcaacag cttctttagg
     5761 caattctgat tctttgatgt cgtaatcatc tgctgaaagt ttgacagagg atagcttatt
     5821 tgacaaactt gtgattgttt cttctgttat ttcagggttt agaaaatttt tttcttttgt
     5881 actatacagc ttgtctactt cttttgataa agaagtcaag gtgtcttttt gattggtgat
     5941 ggcagattct gcataattga tccgttgctg atgttgccgc tgattattga tatggtaacc
     6001 aaacaaacag atcgcaatag cacctgcacc taccgccaca gcagtcaatg acttgacgaa
     6061 tggcttttcc atagattcac tcctaagtat tgatgataga taaattaaga tataattcac
     6121 ttacataaaa atgacagctt tttattttaa aaaacataat taaactaata tattcacaga
     6181 aatatttgta aacaagtttt ccctatataa t
//
LOCUS       DAXLPI010000127         6188 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXLPI010000127 DAXLPI010000000
VERSION     DAXLPI010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.129"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            725..811
                     /locus_tag="ACF3T5_002357"
     CDS             725..811
                     /locus_tag="ACF3T5_002357"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFD3632890.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACF3T5_002358"
     CDS             complement(932..1621)
                     /locus_tag="ACF3T5_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632891.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="ACF3T5_002359"
     CDS             complement(1628..1906)
                     /locus_tag="ACF3T5_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632892.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="ACF3T5_002360"
     CDS             complement(1866..2780)
                     /locus_tag="ACF3T5_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFD3632893.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACF3T5_002361"
     CDS             complement(2762..3142)
                     /locus_tag="ACF3T5_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFD3632894.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACF3T5_002362"
     CDS             complement(3592..3879)
                     /locus_tag="ACF3T5_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFD3632895.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACF3T5_002363"
     CDS             complement(3899..4123)
                     /locus_tag="ACF3T5_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFD3632896.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4593..4763)
                     /locus_tag="ACF3T5_002364"
     CDS             complement(4593..4763)
                     /locus_tag="ACF3T5_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632897.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4778..5311)
                     /locus_tag="ACF3T5_002365"
     CDS             complement(4778..5311)
                     /locus_tag="ACF3T5_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFD3632898.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5332..>6188)
                     /locus_tag="ACF3T5_002366"
     CDS             complement(5332..>6188)
                     /locus_tag="ACF3T5_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFD3632899.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1640 a         1152 c         1042 g         2354 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatttattat ataacaatga ttaacgtcat aaaaagtagt tttgaattca
     4261 taatcgttta aataaaatta taagatcagt tatttaaaca gctagtagac ataatgtgta
     4321 tactaccaag taacgcctgt accaagggat ccaggcgctg attttgtgaa tttttattgg
     4381 tctaggttaa aagctctttt taaggcttgg ttttctgcgg ttgtttcttt ctaattcttt
     4441 gtgaatattg atcggtcatt ttttaaatac ttgttatttt tgtttgtgtt tgttatgctt
     4501 tttacatatc tcatattcat ttattattct tttctaaggt taataatagg ataatctctt
     4561 tcagaaagac tgctcgtacg agcagtcttt tcttatttat caagtttttc tacaaatact
     4621 tcctcatttt gactgttgta gtaattagga ttgagtttta gttccccttt tttatttcct
     4681 ttgttggcca ctgataataa cattgggcta aagcttaata ttcctagtag gatagaaccg
     4741 aatagcaagt gtttttttgt cataagtatt tccttcttca tttccatagc tgccaccatt
     4801 ttttccaatt ctttgtttgt tcgtccattg tttctgttgc ttcagaaaga gtcgttgaac
     4861 tattctctga agactcttcc gtataagtca actgcagttg ctcctgaagt ttttcgattt
     4921 gttggttggc ttgcagggtg agttgttgtt gctggtcgag tagtttttgt atatgagaat
     4981 tattttgctc tttgagggca agttgttctc gtaaaaattc attttcttca cgcaactgat
     5041 ctagcaactt gtttgcaacc acgtttttgt ttggtatata agcattttta tcgttgtttt
     5101 gttcatgttt atgcaactta tctagcaacc tttgattgtc taagttgcga gcaagttgca
     5161 atactccaga acgcaataca accttataac cacgctcatt tttagctatc atggaagaat
     5221 ctaatttttg gatatgtttc gatattgctt gcctactcat cttaaaaaga tctgacaatt
     5281 cctttattgt aaaggtttct tgtggttgca tactggttgc ctcctaggtt gctactggaa
     5341 taaattatct aaatcagata ataaactttt tttctctttt tccgaaagtg cggactctgt
     5401 ttgtttttca agagcttttt tctctttttc tagttgtttg atttgttgga ggagttcaag
     5461 ttctcgtttc ttctgtttga ttttttcgtt aattgttgta gcgggttctt cgtattcttt
     5521 ttgtttatca gctaaagcca atgctttatt ttggtcttct ttggttggtg catgtttgtt
     5581 aaagtcgtct gcttttttga tctcaggttt gaacgtaaat tgatagccaa taacaggttt
     5641 tccccgtcct tttccgtatt tctttttgat tgttagtcct ttgaagattg cagttagttc
     5701 ttgacgtata ggttttataa ctcgtttatc tatatcaggt tgcttataac ttttaggaat
     5761 atctaataat tcaagaaagt cttctttgga gaaatatgcg aaacctttgg ttctgtattg
     5821 ctttaacaga cgaaacatag tctttgcata gccactttgt agctcgttga attgttgtaa
     5881 agaatagcgt acccactcgt ctaaattatt taacaaaggc agtgcttttt cgtaaattcg
     5941 tatatctacg taaggatttt ctacgtcccc gttgatttta aattctgtaa acattacaaa
     6001 catatcgtaa gaaagcccag actcacttct acgaccaaag cgtaaagata agattttttc
     6061 ataagttgct tttaaatctt ctacaaatcg tacatttgca gttgctttgt agttacttaa
     6121 atcttttagt tggtcaaacg taaaacgaac tgtcttatcc cctttttcac gcattctaga
     6181 cacgatgg
//
LOCUS       DAXLPI010000128         6121 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXLPI010000128 DAXLPI010000000
VERSION     DAXLPI010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.131"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            179..1207
                     /locus_tag="ACF3T5_002367"
     CDS             179..1207
                     /locus_tag="ACF3T5_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632900.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(1233..1673)
                     /locus_tag="ACF3T5_002368"
     CDS             complement(1233..1673)
                     /locus_tag="ACF3T5_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFD3632901.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1833..2339
                     /locus_tag="ACF3T5_002369"
     CDS             1833..2339
                     /locus_tag="ACF3T5_002369"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFD3632902.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2507..4419
                     /locus_tag="ACF3T5_002370"
                     /pseudo
     CDS             2507..4419
                     /locus_tag="ACF3T5_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            4424..5425
                     /locus_tag="ACF3T5_002371"
     CDS             4424..5425
                     /locus_tag="ACF3T5_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFD3632903.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5627..5833)
                     /locus_tag="ACF3T5_002372"
     CDS             complement(5627..5833)
                     /locus_tag="ACF3T5_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFD3632904.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2263 a          935 c         1212 g         1711 t
ORIGIN      
        1 aaggtactta tcaatcctta gaaaatctta gtcaaaaaat tatataagtt tgcgtacgat
       61 tcaaagaaaa ttaagagaac ttaatactat tttgaaaaac tatcatttat cttgcagctt
      121 gaaaaaaggt aatccgatag aaggagaaga ataccgtatc cgatactttt ttcacttgat
      181 gtattggcag tcattcgacg aaaatcattc tgatatatta tcaagtataa aaaataaacg
      241 tcaactatta gaaaaatttg cgaaacatgc gccatatacg cgtagtatag atagaaagaa
      301 atggatcagc ctactaagaa tttcgttgca acgaatgaaa caaggccact cgattacgta
      361 tgtcccagaa gagatgaaca aatttgtgca tccattgata acaaaagaag agtttacgca
      421 ttacatacta gagccttttt ttgaagcaaa cttctggtcg atgaatcaca tcactccaac
      481 agagcaagct tacctttatt ttatgtttgg tgtaatgaac acctatttgg aagaagattt
      541 gttggatact ccagataaag tacaagaaag ttggggctgg attgaatcgt ctattgtaga
      601 aaattttgaa aaaaacttca agatttgttt tacaacttca gaaaaaaatt atctatatgt
      661 caatttggca atgatccatt tgtattcaag ggtttttggg acaaaaaaga aggtggatgc
      721 ttttggaaaa tcagcagaag acagagattt tcagcatata tttccagtga tgtatccaat
      781 catgcaacag ttttttagtg gggtagctcg cagaattcca gagcttaata cttattatga
      841 aaagaacaga cggttgattt ttcaatactg tatgttagtc agggatattt ttataaaaca
      901 cgaacaacct gtcatattct atattcaatc aaagtatggt aaaacacagg aattatgggc
      961 aaagaaaagg atattatcta tgactgaacg cccaataaaa tatagtgcat ccgctgatca
     1021 gttacctgat attgtcatta gcgattatcc tatcgacaaa aaggcatatg atcaaacgaa
     1081 tacacagata ttttactgga atggacaacc tacaaaaaat gattgggcgc gtctgaaagc
     1141 tgaaatcata aaaatcagga atgagaaaaa tagatccata ttggaaaaat tggatttcgt
     1201 taattaagaa aagcagattg tttatgccaa aattaagaca ttgcaaaaag attattttct
     1261 ttaaaatcga caagatgacc agaaagcaag agttcgttga gctcttgctt tcttttagtt
     1321 ttagaaagga acatcctgat tttatcttcg ctaaatccag tcaaaagttt tgcagattgg
     1381 tcgtagataa tcggtttttt caataaagaa ggggatgtac gaatctcaat atatagttca
     1441 ctcaaactga gttcatctat tctgtgaaca agatttttat aactaataga atgagtagag
     1501 agcatttcgg tcacgccgtt gtttgtcaat tttagtaagt ggataaattc atttttggtt
     1561 agtggttgac gaattaagtt ctgttcgata aacggtatat catggacgat aagccaacgt
     1621 tttgcgcgtc ttgaagacga gcagccagaa cctgtataca atttaatcat catacattcc
     1681 tccaatagct aagtatttat gaaataataa catgaagaaa tagtcgaaaa gtgccattta
     1741 gaggtgtaaa ataagaaaat gttgtcacgt ataatggaac tagctagata agaaatacta
     1801 ttaaatcaag aaaaaagaaa gaaggcacaa gtatgtcaaa cacagccgac gcaatagtag
     1861 tgatcgattt acaaaaaggg gtatgttttg attcttcgcc gatacacgaa ttagaaacag
     1921 tgacagcatt agtgaataaa agaatcaaaa catacgaaac gattgggaaa ccagttattt
     1981 tcgttcaaca cgaagatgct caattagtgc cacaaacaaa accatgggag ctattgtcgg
     2041 aattatctat tcctgagcac agttattttg tgagaaaaac ccatgcgaat tcattttata
     2101 agactaattt gagtgtttta ttaactgaat tggactgtca ttcgatcgaa ttatgtgggg
     2161 cacaagcaga gtattgtata gatacgactg tgaagtttgc tcatggatta ggataccagc
     2221 ttatgatgtg tagaggagca acgacaactt atgataatcc ttttatgaaa gcaaaggaaa
     2281 caatcgcttt ttacgagaaa atttgggata aacgatttct gacatttttg gagcaataag
     2341 ttgtttcttc gtttaaaaaa agcagaatgg atactggttt atccattctg ctttattacg
     2401 taaaatgaga aaataatcct gttctatttt tatgggataa accatttatt ttttgtataa
     2461 aaaatgtttg cgtttacata taattctcct agaaacataa aaaggagtga atagcttgag
     2521 aacaaaacaa gtattaaagg gagcgttggt catcttattt gctgtaagta caagcgtccc
     2581 gcaatggaca gtatatggag agagcatttc ttccgaatcg gaatctcggg aggtagaaac
     2641 cacagaaact actgatgctg gaaatgaaac gaaatcgagt acttcacaaa cagaaacaaa
     2701 tactgaaaca acggttgaga catctgttac tagcacagat caagaaacga ccgctaccac
     2761 agataaccaa caaacagaag aagcaacagc tacaaaaatg tcaaacgacg aatcctcttc
     2821 tgctaaagta agtgacaaag agaatgggac agaaaatgat gatatcggta aaggtgaaaa
     2881 tttcaatggt aaagaatatt tgaattactt cggtaaacag atgacagaaa tacttaatgg
     2941 accaacagaa gacatcaagt caaaattaat agagttatta gtcactgagc ttgctgttaa
     3001 tgattatggt gtagataaag agattatcaa acatctgatt gctacttatc tattgtacga
     3061 tatcgctttt gcaaaaaaca gtgtgttgat gtcgatgctt ggaggtacag atgatgggac
     3121 aacgcctaca gaaaacacag gcttctatcc aaatacaaaa caacttcatc aaactgtact
     3181 aaacaaacaa gatggcaaaa tgatggatgt atatgcatat tatgtcgatc aaggttcaga
     3241 taaaacagtc atgatccatg gcggatttcg agggaattgg aataacggga tcgttacaga
     3301 agaatacaat gatttttata aggccgggta taatttatta tttgttgatt ccagagcgac
     3361 tggtaatagc ggaggcgatt atgtgaccta tggacaatat gagtcagatg acgtccttta
     3421 ctggataaat caagaagttc gagagcggcc atctcaaaag attttacttt atggtggttc
     3481 gatgggagca gctacgatga tgtctgtatt agcgaaagat attcctgtca atgtcaaagg
     3541 aattattgaa aattgcggat ttgcatcaat tgatgaacaa ctacgtttta cgtacagcca
     3601 gacagtagcc cccgcactac cggatgccat aaaaaatcag ttagacatca ttggggatca
     3661 agagcatgaa gatttattta tgggcctttt gaaacaatac tattttgatc aggagatgca
     3721 tttggataca aaagctgctc tacctacgat tggaatgtca gattcattgc ctaaattgat
     3781 tatccatgga acagcagatg atgtagtacc tgtgtctaac gcgcaaaaat tatatgagtt
     3841 atcaggaggg tataaagatc ttttattagt agaaggagca gggcatggaa aggcacagga
     3901 agttgaccat gctgcctaca cgaaacatgt aacggacttt ttgaaagtag tgttccatga
     3961 tcagataaac gtaaagtatg tggatgaaaa caataaaagt ctactcaaag accaggacga
     4021 aatccagctt tacggagctt atggagaaaa ttatatgaca gagcaaaaga cattcgaagg
     4081 atacgaatta gcaaatgttg aaggaccgac tgaagggatt tttaatgaga cgataccaac
     4141 tattattttc tattacaaaa aaatcccagt agctccacca aaaaagcaag atccgacaga
     4201 gaatgcagac aataagggaa agaatgaacc tcgagaggat caatcaagtg atgatacgca
     4261 accgattcag atgacagtga aagtacaaaa aaagacagat gcaaaaaaag aatgttgccg
     4321 aaaactggag aaaagaaaca ttcgcttctc atttattcag gtggattttt atctgttagc
     4381 acgattatca catggatttg gcgaagaaag agagggtaaa tgaatgaaat ggttcaagcg
     4441 aatagtgatt gcagtaccag ttatcatcgt agttatttta gtcactgtat ttttgattaa
     4501 tcaaatcaca ccaaagccga ttagcttgct tgttagaaaa caatttgaca caagtgaaaa
     4561 agaacaagta tatgcccgac cagatgattt ccagcaaaaa atcgaacaaa tcgatataaa
     4621 aaaagaaata tcctatcctt caaagtacaa aaataataat atggatatct acacaccgaa
     4681 agtggaaaag aaaaaattac cgattctttt ctggatgcat ggaggcgcat atgtaggagg
     4741 ggataaaaat gattgtcgtg attatttgga atatctatgt tcagacacgc agcaaatcat
     4801 cgtaaatata gattatgaac gatcaccgga agcaaaacat cctacaccag ttatccaatt
     4861 gaacgaagcc attcaagcag ctaaaaaaga gataaaagat caagcagatt ggtcgaatat
     4921 ttcgattggt ggaaattcgg caggtgcaca aattagtgga gaatatttgt tagcacttca
     4981 aaatgaagaa attaaaaaag ctgatcatct tgatccgact ttggagaaca agcagattac
     5041 gaagtttatt tctttgtctg gtctgttgga tccttctgca cttactcaag taagtgataa
     5101 aatctctagc tttctttatg aaaaatgtgg atgggcttac tttgataata aggactttga
     5161 acaatcagag aaggtcaaaa gtttagcctt ggtcagacat gcggatcgtt ggacgcaaaa
     5221 cacattctta acggatggaa atacaaatac gtttacgaaa caaatggaag aagtaacttc
     5281 tgtatttgag aaagcggggg tgaaagtcac aaaagtagat tatgcggaga aaaatgctgt
     5341 tttgaaccat gaatatcagt ttgatttcag caataaacaa gcgcaagaaa catatcaaaa
     5401 gttagtaatg tttttcaagg agtaaagtct tatgatgcaa aagaggttgt aatctgtcac
     5461 ttagctccaa gtcacaagga gcaatttcaa taaacaagct tttcatggtt tatgaatatt
     5521 gtaacttgtg actgaggagt tagagatggc aaggggcacc ttttattcga aatttgggaa
     5581 tatcacacga ttattgtcac gatctctttt acgtaaattt tataagtcag atttgttgtt
     5641 ttgatcgttt aaaaaaagca acaaatccga agcctgcgga ggcaagaagg gacaaaaagc
     5701 cgagtggtaa gcaaaagaat gctggttcca ctaatattcc gttgttattg atataaggct
     5761 gggaacaaag atgaatagca aataaaatca atgcactagc gaaaaaaagc aaagaaatat
     5821 ggatagcttt cataagcagc acctcctttt ttataattgt atgataaata gattaagaat
     5881 ctataaatgt gacaagtttg ttaggatgtg aataaaatga tcatctagcg aacttgaaac
     5941 aaagatggct aatgataagc aattaatgat tcagcaatca gataggtaca aagtttacca
     6001 tcatacgtct agaccaataa tagaaaatat gggaatataa tagaaaagat ttaggatacc
     6061 cccaaaagaa tttccccaaa aattcgatgt atcctaaact tttttatttt acataacaat
     6121 c
//
LOCUS       DAXLPI010000129         6003 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXLPI010000129 DAXLPI010000000
VERSION     DAXLPI010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.132"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            60..758
                     /locus_tag="ACF3T5_002373"
     CDS             60..758
                     /locus_tag="ACF3T5_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFD3632905.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(926..3565)
                     /locus_tag="ACF3T5_002374"
     CDS             complement(926..3565)
                     /locus_tag="ACF3T5_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFD3632906.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4184..5215)
                     /gene="queA"
                     /locus_tag="ACF3T5_002375"
     CDS             complement(4184..5215)
                     /gene="queA"
                     /locus_tag="ACF3T5_002375"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFD3632907.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            5408..>6003
                     /locus_tag="ACF3T5_002376"
     CDS             5408..>6003
                     /locus_tag="ACF3T5_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632908.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         1717 a         1300 c         1141 g         1845 t
ORIGIN      
        1 cagaaatatt ttttgataca tggtatattg gtgttattaa agaaaaggag ggaaaaatca
       61 tgcaatcttc tgcagaatta aaacgccaag caaaagagag tctaaaagga cgttggggac
      121 aagctgtttt actaaatcta atccctacat tgattggtat tgctactatt cttcttatta
      181 tgattcctat tatcttgttt gccaccacca tatatgatgg atcagcgagc gtctcagaag
      241 cgacaacttc aggaggcggt agttcaggag gcggtggcgg aattatttcc agcatcatta
      301 gcgctttgtt catgagcggt atttcttgga cttatcttga tattttacgt ggacaaaaag
      361 atcagatcga accattcaaa gatgcatttc gtggattcaa aggtttgttt atcggcggag
      421 tgatcagctt agctgtgctg atctcgatct ttacaacatt atggggtttg ctattagtta
      481 ttcctggaat cattaaaagc tattcttatt ctcaaagcta ttttatttac tacgatatcg
      541 tccaagaaac aggacaaaca cctaaagtac tggatacgat cactgcaagt cgaaaattga
      601 tggatggtta taaaggacaa cttttctggt tagatctttc atttatcggc tggcatatct
      661 tagcattagc aactgctggt attggttatc tatggctcag cccatacatt tctgcaacaa
      721 aagcagcatt ttatgaacaa ttgccaaaag atatttaaaa gagagtgtga cttgtgaccg
      781 agggggtggc aggagaacac cgtttatcga gaggcagtaa aagaaacatt cagcccatta
      841 gcaaaaagga ggtgtgacaa cattcatgtc acacctcctt tttgtatagt tccagtaaaa
      901 agaaaatcaa cttagaaaca gttaatcatt cctcttcttc tgcttccggt aatcgatgga
      961 acttgatcag ttcattcacc gcgattggta cgatagataa aatccctacc aagaaccagt
     1021 gattcaaact gatgtgtact aggccgaata gttcttgcgt aaatggcagc aaagcaacag
     1081 ccgttgtaat agctaatgcc agtaacgcca tttctaccaa agctttattg gtcgctaaat
     1141 tgactttgaa gatcgattgg ctgctgcgta cattgaacac atgcagaatc gatgaataag
     1201 ctaagatcaa gaatgcaaca gtttgaccga catgctgact tgcgtctacc catgggctga
     1261 tagtatcgac ataactacct aaataataac caaacaaggt tacgatggtg aagacccctg
     1321 catttatggc aattttttgc aataaacctc gagcaaagat cccttcattt ttcggaatgg
     1381 gtggctgatc catgatgccg tgttccgctg gctctcttcc taaagcaaat cctggaagtc
     1441 catcagcaac tacattgaca aacagtagtt gcacagctgt aaatggtgct ccccatccta
     1501 acagcatcgc aatcaaaaca ataaagatct ccgaaatatt acagcttagc aaaaagttga
     1561 tagctttacg aatattctga taaaccgaac gtccttgact gacagcgtct acgatcgtcg
     1621 caaagttgtc atccgtcaaa atcatatctg cggcactttg cgccacatct gttcctgtga
     1681 tccccatggc acacccaacg ttacttgctt tcaacgctgg tgcatcattc acaccatccc
     1741 cagtcatcgc tacgactgca cccgtacgtt gccaagattt gacgatccgg atcttgtctt
     1801 ctggcgttac ccgagcatag acagagagag ctttgacacg cgcatctaat tcttcgtctg
     1861 ataattgatg cagttctgat cctgaaagtg cttctgacgg atcattcaag atacctaatt
     1921 ctttcgctat ggcactggca gtcaccacat gatctccagt gatcatgact gttttgatgc
     1981 cagccttttt cgctcgcttg attgctcctt tactttctgg acgtggtgga tcgatcattc
     2041 cgatcagacc aatcaaacgt aagtcttttt ccagtgcttc agaagaaata tcttgcggtt
     2101 cttcgtcata agtagcataa ccaacagcaa tcactctcag tgcccgttta ccaaagcgat
     2161 cattaacgat cgccgcttgt tctacatcgc ctgaactaaa gcgcggagca agaacatcga
     2221 atgctccttt ggtgattgag atgtattttt cgcccatttg gtgaactgtt gtcatcattt
     2281 ttcgttcaga atcaaaagga agctcgttta ctcgtggata tttttcttct aattctgcct
     2341 ttgtatgata attctcttca acagccctta cgatggctac ttctgttgga ttacctttga
     2401 taacaaggtc gtcatcttct tgctcgaccg tcacatctgt acatagtgca gccattttca
     2461 atatttccat cgcgctgttc gtcattgaat cttcgatatt cgttacttca tcttctttgc
     2521 tccatacccg gcggacgcgc atttcatttt gtgtcagcgt tcctgttttg tctgagcaga
     2581 taacattagc ggttcctagc gtttcaactg ctggcaactg tcggatgatc gcatgtttcc
     2641 tcgccatctt ctgcacaccg tatgcaagtg tcaacgtaac aataacagtc aaggtttccg
     2701 gtacagctgc aacagccaag gaaatcgaag tcatgaacat ctcaaggagt ggttctcctt
     2761 gcagttcacc aatcacaaat actaaagccg cagccgctaa agcgatcata ctgattcgtt
     2821 tgcctagctg attcaatcgg acctgtaaag gtgttttttg catagtattt tcgttcaaaa
     2881 gatccgcgat cttacccatt tctgtagcca tgccaatggc agtgacgatc gctttcccac
     2941 gacctgcagc aaccgtacat cctttaaata ccatattaag ttggtcacct aatgactcat
     3001 cttcttcagc aataaatgct gcatcttttt caactgcttc actttctcca gtcaacgccg
     3061 attcttctac acgtaattgt gatgcttcga tcaaacgggc atcggcagaa atagcatcac
     3121 cggactctaa aacaagaata tcacccacta ccagttgctc tgactcaaca gtggtcactt
     3181 tgccgtctcg tatgacagtt gccgtttgtt tattcatatc ttgcaaggat tccagtgctt
     3241 tttctgcgtt tccttcttgg acaatcgcca ataccgagtt gatcacaacg atagcgatga
     3301 tcagcagtcc ttcaaataaa tccccatgtt ctgtagcaaa tgcggtataa aaggaaatca
     3361 ccgctgctac aagaaggatg atcgttgtaa aatcggacaa gcttcgaaag aattttttga
     3421 tcatcgattc tttcggcgcc tcatcgaatt tattcaatcc ctgctttttt cgccgttcat
     3481 ccacttcttg tgaagacaac ccagtatctg gatctgcttc tgttaccttc tttacttctt
     3541 ctattgtctg cttgtattct tccatcctat tcctccttta aaatcttcgt acaaaaaaag
     3601 gtacttattt tactataaca tccttttttt atttacttca aataaagggc ttatcgatta
     3661 tttgttaaga aagcgtaaaa tttaaagtgc ttatatttgc aaacgctacc aaaggatgct
     3721 attatataga tgtaaggaag gaggatacca tcaaatagac agaagaattt ctcgatagaa
     3781 cagtgtaagc aaacaaagtt gttagaagta caggtttcac ccagacatgc gtgtagagta
     3841 caaacctata aatagtgaat gcgtatggat gtttggatca cgaagtatcc gaaacaagaa
     3901 gtaatgcgat acgtaacaat tgaataacgt aaacgtctga aaattgaata ggaacaagag
     3961 atgtttcaaa tgttgaagat gtaacgaaaa caaaattttt gtttaacatg aagaaattca
     4021 acaccgtgct ttgcatgtat gatggaaagt tacaatcatt tctgtttatt tgaatccttc
     4081 cgaggtacaa gaaagccgag cttatcgaga gctcggcttt ttctttgtca taaaatcttt
     4141 gtaaagcttt tttgttattc aaaactagcg gcttttcata accttatttc acgaacatcg
     4201 catctccaaa actaaagaaa cgatagcgct cttcaatcgc atgctggtaa gcagataaag
     4261 tcaattctct tcctgcaaag gcactaacta acatgactaa agtcgatttt ggcaaatgga
     4321 aattagttga gaaagcttct accactttga attcatagcc aggcgtaatg aaaatatcag
     4381 tccatccgct gtctgctttg atttctccat taaatttcgt tccaatcgtt tctaatgttc
     4441 gaattgaagt tgttccgaca gccacgattt tcccgccatt tttacgtact tggttcaatc
     4501 gctcagccgc ttcttccgtc aaacggtaga attcactatg catatggtgt tcttcaattt
     4561 tatctacgct gacaggtcgg aatgtaccta accctacatg tagtgtcaga tagactaatt
     4621 caacaccctt tgcttcgatc tcatcaagta attctttcgt aaaatgcaat ccagctgttg
     4681 gcgctgcagc agaaccattt tctttcgcat agacagtttg atagcgatct gggtcttcca
     4741 gacgttcttt gatatatgga ggcaatggca tttctcccaa tgattcaaga atctctagaa
     4801 aaattccttc atactcaaag tcaatgatcc gtccgccgtg ttccaattct tctttcacta
     4861 ctgcttgcaa tctgccatca ccaaaagaga ttcttgtccc aactttggca cgtttcgcag
     4921 gtttgatcaa tgtctcccac gtatctcctt gtgtattcgt tagaagaagg acttctaaat
     4981 gcgcaccagt atctggtttt tctccatata aacgagcagg cagaactcgt gtattattca
     5041 taactagcgc atcaccaggc tgtaactctt caatgatttc atggaaatgt ttatcttcca
     5101 tctctccagt tttccgatcc aaaacaagta aacgagaatg atcgcggtct tttaatggtg
     5161 tttgtgcgat taattcttcc ggcaagtcaa aatcaaaatc ttctgttgtt aacatatcgt
     5221 cactcacttt ctatcatgtg cttcatcatt ctatcatgtt tttcgttctg ttggcaaaat
     5281 cagcactttt tcacttctat tttttgtttt cctaaataaa aaaactaccc tatttgtatt
     5341 ttttcattgt tttaggcata ctagtaatag atagttcttc attgaaaata gaaaaagagg
     5401 tggcaccatg attgaatttc aaaatgtgtc aaaaatttat aaaggtggaa aagtcgctgt
     5461 agaggacgtc aatctttcat ttgaaaaggg tgagttcatt tgttttatcg gtacgagcgg
     5521 aagcggaaaa acgacgacga tgcggatgat caaccgaatg actgatccaa cacaagggaa
     5581 aattttgatt gatggaaaat ccatccaaga aattgatcca gtggaattgc gacgtcagat
     5641 cggctatgtt atccaaagta ttggattgat gcctcacatg acgatccgtg aaaatatcac
     5701 tcttgtccaa aaattgttga aagtcagtca agaagaacgg aataaaacag ctgaaaaaat
     5761 gattgatctt gtggaattgc cccgtgagat gttagatcgc tacccacatg aattatctgg
     5821 tggtcagcag cagcgtattg gagtcgttcg tgcccttgca gcgaatcaag atatcatctt
     5881 gatggacgag ccattcggtg cgcttgatcc aatcactcgt gattccttgc aagaccttgt
     5941 caaagattta caagagcgtt tgggaaaaac gatcgtattt gtaacccatg acatggatga
     6001 agc
//
LOCUS       DAXLPI010000130         5976 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXLPI010000130 DAXLPI010000000
VERSION     DAXLPI010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.133"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..1381
                     /locus_tag="ACF3T5_002377"
     CDS             <1..1381
                     /locus_tag="ACF3T5_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFD3632909.1"
                     /translation="CGQFYVNKEVNLEKLSNLLHVCKTTLLNDINNIKKEFEEQIAYT
                     HREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSK
                     AYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERA
                     DRFIESVTENSGRFYDKENKEILLIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYV
                     ESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKE
                     LVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQT
                     IFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALE
                     AVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDR
                     AIISAVQNK"
     gene            complement(1473..1838)
                     /locus_tag="ACF3T5_002378"
     CDS             complement(1473..1838)
                     /locus_tag="ACF3T5_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFD3632910.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1835..2200)
                     /locus_tag="ACF3T5_002379"
     CDS             complement(1835..2200)
                     /locus_tag="ACF3T5_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFD3632911.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2392..2640)
                     /locus_tag="ACF3T5_002380"
     CDS             complement(2392..2640)
                     /locus_tag="ACF3T5_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632912.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2639..2767
                     /locus_tag="ACF3T5_002381"
     CDS             2639..2767
                     /locus_tag="ACF3T5_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFD3632913.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2824..4518
                     /locus_tag="ACF3T5_002382"
     CDS             2824..4518
                     /locus_tag="ACF3T5_002382"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFD3632914.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4618..4791
                     /locus_tag="ACF3T5_002383"
     CDS             4618..4791
                     /locus_tag="ACF3T5_002383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632915.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
     gene            5169..>5976
                     /locus_tag="ACF3T5_002384"
     CDS             5169..>5976
                     /locus_tag="ACF3T5_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFD3632916.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKGERQ"
BASE COUNT         1958 a         1135 c         1096 g         1787 t
ORIGIN      
        1 atgtgggcaa ttctatgtaa ataaagaagt caatctagaa aagctctcca atctcctgca
       61 tgtttgcaaa accactttgc tcaatgatat caataacata aaaaaagaat ttgaagaaca
      121 gatcgcttat acacatcggg aaaaggaccg ttacacttta tatttttcag aacatatacc
      181 tcgatgcaag atcatgcagc aattgtctca aaattcgttg tttttaaaaa cttgtctgct
      241 ttatttggaa gaagatgaac ctgattatct tcaattaact gaatgtgaat tcatctctgt
      301 cagcaaagct tattcattaa aaaaacaagt attagcttat tttaatgact gcgggatcga
      361 aatcgaccgc tactcccctc gattcactga aatggaaaga cgtctgctgc ttcttaatgt
      421 gtcgtatcgt ttaggcggtt ttaattcttg ggagctacct gaatcgtttt ttgaacgtgc
      481 cgatcgtttc attgaatccg taacggaaaa tagtggcaga ttttatgata aagaaaataa
      541 agaaatctta ttgattggtt ttgctatcag tttcctacgc cagcaagtgt gtgctgtgac
      601 aatagattca aaatttatcg aagaaatcaa aaaacgtcct atttacaatt atgtcgaatc
      661 agcatgggaa aacactgatt tccaaaccta ttataaaaaa gaagaattcg cctttatttt
      721 gacactattc aatctttgta attatggttt ccattcttat cagttgattg cggaagactt
      781 tcaacagcta caccaagtgt ttattgacaa tactcccgaa ataaaagaac tagttgctac
      841 ctttgaatct cacttcaatc aagaattatt cggaaaccaa ccattcgaac gtgcgctgat
      901 tcatctgatg cgttcggctt gggataacta tcaattattc atgccagaaa aattttatct
      961 tctaaatgag gaacagacaa atttatataa agaagtccag acaatttttt ccagctggtc
     1021 cagccagtta ccttatgatc tacggttgaa tcctaattgc atgcgggctt ttgtgatcga
     1081 attgagcgga attctccgtc tgacaaaaga acatctgact atctatatcg tcacaaattc
     1141 ggatgttcat tatctgattt atcgggaagc cttggaagct gtaactactt tcgattttca
     1201 agttgcacca acaatttatt caagtatatc tgatataaag aagtatgcgc agcagtcctc
     1261 aaaccgggta ttatgtgaac gtacattata tacaccagat gcggtccaat atgaaaatat
     1321 tattcctatt tcgatcaata cgatcgatcg agctatcatt tcagctgtgc aaaacaaata
     1381 agggactgtg acaaaaatca tgtcacagcc tcttatttgt tgaagatgcc catttctgta
     1441 acaatctctt gatacacatc aggctcacaa cctcacttat tttggacatc tacgggtccg
     1501 atctctttat ttaaaaaata agaaatgatg gttcggataa ctaccaacgc cgctagttgg
     1561 aaaatatcct gcaaggtagg tttgataatc gattcgatga tcccagctga aacaagaatt
     1621 tccaaactta gaagtatata gcctcccaac atctttttaa tagcattgtt ctcgctgact
     1681 cggtcgcttt gcacttttgt atggacaaat aaatatttca aaaaatcctt gattgataga
     1741 caaacacccc aaaccaataa acagatagca aagatatcaa gtcccagtac aagtttttca
     1801 aaatatggaa gaagaaactg catcaactct gtcgtcatcc tacctctcct cttctttgtt
     1861 tttctttgtc ttctatttcc tgattcaaga aataagaaat gactgttcga atgataataa
     1921 taagtgcaag cttcaaaata tctgtccaag tcggcttgat aactgactcg atgatatcag
     1981 caacgactag tacttccaag cttaacaaaa tataacttgc tagcagtttt cttgtatctg
     2041 cattacgagt attacgttct gcatagtttt tcttgacatg agaccaatca aaaagtttga
     2101 atagaacaag aacgatcccg acaattaaga taccgataga acaaatattt aaaatagcaa
     2161 tgatccattc tagaaattca attaaatttg agcgaaacat atacgctacc tactttcttt
     2221 taatttgttc ctattctttt attgtaaaag aaagttacct tgttcgtcta tttaaaagtt
     2281 gttctaatga taacggttcg cttaaatttt tctttttcac tctccccttt ttctccctcc
     2341 atccataaat cgtggaagta tccaaaacaa cgtagcaagt taaaataagg cttagtccgt
     2401 catcagcttt tcaaagaagc actgtctcgg tgcttttttt gtacccaaat ttctaaaaat
     2461 agaaccatac acctcctcaa atcttttctt cttacctacc ctatttttat ttacaggaaa
     2521 atcagacact tcttcttttt tgtcaaaatt tcctttttct tctcgtccta tcatttggca
     2581 ttctaataaa aacgaactat ccttggatat agaaagagaa aacaaggagg cgtttttcat
     2641 gagtaaaaaa ttaatcaaag tcggcatcgg tttaggtttg cttgcccttg gcgctgcata
     2701 tctaggaaaa aagacaggac tattcgaaga tgacagtcat ttatatgatg aattcgaatc
     2761 aatctagcat cctatttagt tgaatcacta cggacatact tggaagacat tggaggaatc
     2821 acaatgagaa aaagtaaagc aataatgatt ggtgccggta ttgccaatat ggcagcggct
     2881 gtttatttga ttcaagaagg caaatggaaa ggggatcaga tcacctttta ttcattggat
     2941 gatcacggtt caaatgatgg tgcaccgacc gacactgtag tggatgaata ttggaataaa
     3001 aaccatccat tagaaaacca aaaaggctat attgctcgag gcggtcgcat gcttaattac
     3061 cgtacctatg tagatttgat ggatctgtta agtcgaatac cttctgttac tgagccaggg
     3121 atgactgctg aagaagatac gagagatttt gatgcaaaac accgaacctt tgacaaagca
     3181 cgtttgatgg aaggtggcaa aggaatcatt gatggcggaa aactgggatt caataataaa
     3241 gaccgtattc ttctgactaa actgattgct atgcctgatt cagaagaaga aaaactagac
     3301 aatatcacta tcgaagaata tttcaaagat gatccacact tttttgaaac aaacttctgg
     3361 tttatgtggg aaacaacatt tgctttcaga acacgtagtt ctgctcaaga attgcgtcgc
     3421 tatatgcatc aaatgattta tgaattcact caaatcgaac atctagtggg cgtcaatcga
     3481 acacgttaca atcaatacga aagtattatg cttcctttga tcgagtactt gaaagaacaa
     3541 ggatgcaaaa tcattttgaa ccgccgtgtc actgactggg aattcaaaga aacaccgatg
     3601 caagatgaaa tcactgtgac tggtttgaaa atgaccaata cactgactca agaagaagaa
     3661 cacgttgtgg tcgatgaaga tacagcagtt attttcacta atgggtccat tacagattca
     3721 gctactttag gagattacga aacaccagct gtagaaaata tggattatgg tgcagcttct
     3781 agtctctgga aaaaagccag tgaacgtttc tacaacttgg gaaatcctga caaattcttt
     3841 gctgatcgag atgcaagcga atgggtcagc tttaccctga cgacgaaaaa ccatttgttg
     3901 ttgaatgaaa tcacccgtat cacgactcaa gaaccaggaa atgccttgaa ttcatttatt
     3961 tcaaccacac cgatcacgcc gttaggccaa aaagatgtca atatgtctat cgtcgtgcat
     4021 catcaaccgc attttacgtc tcagaaacca aatgaaacag ttatttgggg ttatttcctt
     4081 tatccaagac gacgtggcga atttgtggat aaacagtata ttaaaatgaa tggaaaagaa
     4141 atgctagaag aactgattgg acaactttca aaagtagatc ctggaccagt gaatattcgt
     4201 gaaaaagaaa cagaaatttt cgacagcgta ataaataata tccctgtcta tatgccttat
     4261 gcttccgcgt tgttcaataa tcgtgccaaa agcgatcgac caaaagtcat tcctaaacat
     4321 tcgaccaatc tggcctttac aggagaattt gtcgagcagc cttatcagat gatctttact
     4381 gaacaaagtg ctgtacgttc tggagaaatc gctgctttcc attttgcggg tatccctatg
     4441 tcacgtcttg tcaaaacacc gcgttacgac aaagatattc ctactctagc tcgtgccgca
     4501 aaaaaaatgt ttgaataatc aagaggttgt gaaaaagctg ccctgcatca agtaataaga
     4561 acaaccaggc taaaacagct tcttatgttt ttgatttggt tggacttatt accgcagatg
     4621 caagcttttg aacaccgttt accaagaggt tgtgaaaaag ctgccctgca tcaagtaata
     4681 agagcagcca agctaaaaca gcttcttatg tttttgactt ggttggactt attaccgcag
     4741 atgcaagctt ttgaacaccg tttaccaaga ggttatgaaa aagtcgttta gtttcgagta
     4801 tgaagaaaaa ttttgtgaaa atggttcctc acgtttttgc ataaatttga cttaataccg
     4861 agaaactgat cccttctgtc ataacctctt tttatttatg tctgtttctt tttgaaaaca
     4921 accatttttt caacgtttac tctcttgcca atccaacatc atctaacaga tacaactatt
     4981 tcaatgatta ccattacact aaaaaatcgg aaactcacct ctccgaacac aaatacataa
     5041 gactatagaa tgtttcattt aagaaaaaaa gcgtttcatt atcaaaaagg taagatctcc
     5101 tttaaaatga aattatctgt agtactaata atgcagacaa ttttatgaat ctgaaaggag
     5161 catctccaat gaaagcagtc acttggcaag gaaaacagaa aatggaaatc agaaacgtag
     5221 atgatccaaa aatattagaa ccaacagatg caatcatccg tatcacagca acagccattt
     5281 gcggctccga tctgcatctt taccatcatg gtgaagcagt tttggaaaaa gggtatgttg
     5341 taggacacga accaatggga atcgttgaag aagtgggatc tcaagttcaa acattgaaaa
     5401 aagggcaacg tgtggttatc ccttttaata ttagttgtgg tcattgtcat ttctgcctga
     5461 atcacatgga aagccaatgt gatgaatcaa atccagatca gttatatggc gggcttttcg
     5521 gatttggcaa gttgaacggt aatcattggg gtggtcaagc ggaatatcta agagttcctt
     5581 atgcagactc tacttcattt gttgtaccag ataatgatct tcctgatgaa aaagttttat
     5641 ttctatcaga tattttgccc acagcttatt ggagcgttga aaacgcagga gtaaaaaaag
     5701 gcgatacagt tgtgattcta ggttctggtc cagtcggtct attcgctcaa aaattcgctg
     5761 ttatggctgg agcagagcgc gtaatcgcag tagatccagt acagcaccgt ctagataaag
     5821 cggctaatta caatggggtt gaaacttatt tcttagaaga cacggcacaa gctggaacag
     5881 aattgtatga actggctaaa ggtggcgcag atgtcattat cgattgtgta ggaatggacg
     5941 gattagagcc tgtcaaagaa aaaggggagc gtcaat
//
LOCUS       DAXLPI010000131         5947 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXLPI010000131 DAXLPI010000000
VERSION     DAXLPI010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.134"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..416
                     /locus_tag="ACF3T5_002385"
     CDS             <1..416
                     /locus_tag="ACF3T5_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3632917.1"
                     /translation="DTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHCIHFVLYS
                     DKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQGYNTILLG
                     AQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(494..1027)
                     /locus_tag="ACF3T5_002386"
     CDS             complement(494..1027)
                     /locus_tag="ACF3T5_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFD3632918.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1121..2299)
                     /gene="mutY"
                     /locus_tag="ACF3T5_002387"
     CDS             complement(1121..2299)
                     /gene="mutY"
                     /locus_tag="ACF3T5_002387"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFD3632919.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2292..3089)
                     /gene="recX"
                     /locus_tag="ACF3T5_002388"
     CDS             complement(2292..3089)
                     /gene="recX"
                     /locus_tag="ACF3T5_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFD3632920.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3196..4578
                     /gene="rlmD"
                     /locus_tag="ACF3T5_002389"
     CDS             3196..4578
                     /gene="rlmD"
                     /locus_tag="ACF3T5_002389"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFD3632921.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4752..5330)
                     /locus_tag="ACF3T5_002390"
     CDS             complement(4752..5330)
                     /locus_tag="ACF3T5_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFD3632922.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5652..>5947
                     /locus_tag="ACF3T5_002391"
     CDS             5652..>5947
                     /locus_tag="ACF3T5_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFD3632923.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFF"
BASE COUNT         1758 a         1175 c         1067 g         1947 t
ORIGIN      
        1 aagatacaat gagcaatatt tacttagatg ctgtacggat caggcatcaa gtttttgtag
       61 tcgaacaggg tgtgcctttg tctcgagaaa tagacaaaga tgaagcccat tgtatccact
      121 tcgtcctgta ctcagataaa aaagaaccgc aaggaactgt acgtcttttg cctttagaaa
      181 acggaaaaat gaaattacag cggatggcga ttctttctga atacagacac caagggttag
      241 gaaaaatatt gattgaagaa gcagaaaact ttgctaagaa tcaaggctat aatacgattt
      301 tattaggggc acagtccaca gctgaaactt tttacgaaaa actaggatat acagcttacg
      361 gcgatccttt tgaagatgct ggcatgcctc atatttatat gaaaaagact ttataaaaat
      421 ttatttcgaa aaaagcaaaa aacagcaagc ttttgagaaa aatctctcac tggcttactg
      481 ttcttttttg tgtttactta cgcgacaatt gcttgtaacg gttataccaa atatcaatat
      541 agccttccga gaacgggcca tctccattat tgatccagtc aaccagaatc ttcacatttt
      601 cttttaggat gaagtcaata tcgttcgggt aattcatcat tttgctgtgc atctcatatt
      661 catcgacatc caatagtcgt ttctcgccgt caggaaatac cttgatatcc aagtcatagt
      721 cgatatattt caaggcctca tcgtccagca aaaaaggtga agctagatta caataatagg
      781 aaaccccctt ttctctgatc attgctatta cattgaacca gtatttttta tgaaaataaa
      841 caatcgctgg ttcgcgcgtt acccatcggc gtccatcaga ttcagtgact aatgtgtgat
      901 cattcacgcc gatcatagag tgttcgcttg tttttaatac catggtatct cgccacgttc
      961 gatgcaattt gccgtcgtgc ttgtaacttt ggatcgtcac aaactctcct tcttttggaa
     1021 ctcccatagc aacccttcct taagcagcat tttgtgcttt gtgtttgggt gttcattaca
     1081 ccgacatcat aattattata tcaataaaaa tgagctttgc ctagaaagga ttcctattgt
     1141 ttcgattttt ctctaactga tctaccagtt tcatttggac cttaggaaaa acgaccgaat
     1201 caaatgcatc aggcttcaac cacttcgaag ttttgttctc ggtaaattct tgttctgctc
     1261 cctcggttgc ccgtccgtaa aaaaggagaa catgccattt cagatgacta aaaatatgag
     1321 taacttctcc taaatgtcgt gtctgccaaa caaagagttc ctgcttttca aatgggagat
     1381 tttgctcttc ttctgctact aggtcatcaa acagatcgat ttctggcttt gtttcccatt
     1441 cttttttcaa tcgctcatac tcctcttgtg tcacttccat cattggaaat gtccacatat
     1501 tcgccagcaa tttttggcta tctctttctt caaaataata cgctccagaa tgattttgaa
     1561 gagctgcact gatataataa acgtcttttg gctttgcttt ttttgttttt acaggaaaac
     1621 tcgtttggat tcctcgtttg ttagctaaac aaaaggcttg gatcggacaa gtctcacact
     1681 ttggagaagt tggtgtacag atagctgaac ctagatccat cattgcttgg ttgaattcac
     1741 ctggatgctt ttcatcaatg attttccgca tcgcttcatc aaagattttc ctgctagaag
     1801 cttttgcgat gtctgcttca atgcaaaaca atctgctgac tactcgcatc acgttgccgt
     1861 caactgcagg ttctggaagt ccaaatgcga tacttgcaat cgcgcctgtc gtatatggtc
     1921 ctattccctt caatgagcta atttcttcag gcgtttgcgg catttcccca tcaaattctg
     1981 acatgatctg tttagcagca gcttggatgt tccgggctct tgagtaataa ccaaggccct
     2041 cccatgcttt taaaagcttt tcttctggag catttgctag ctcttcaatt gtaggaaacc
     2101 attccatgaa acgataaaaa taatcgatca ctgtgtctac tctggtttgc tgaagcatga
     2161 tctcagagat ccagatacga taaggatcac gattatagcg ccaaggcagg tttctttttt
     2221 cttgttcata ccattgaatg aactgatctt gaaattcttt tgtttcttta tctgtccatt
     2281 tttcccattc tttattcact ttcctcttcc ttttccgtaa tgaatcgctg gatcatatcc
     2341 aaatcaaatc ctttttgata taagctttgc tttactttta gatttctttt tttaggttca
     2401 aaccttaaat ttcttcgcca tagtttatct ccttgtttga ctaaataatc atattctatt
     2461 tcttgatctg cttcttgcgg aagatcagct atcgcttgtt ggatcacttc ttgagaaaag
     2521 cctttcgtca tcaatgtttg ttggattttt ctcaataatt ctttttggct ttttccgtga
     2581 ttttttccaa acgctttttc ggctgtctga tgtgcatttt gaacttggtc ttcaaatgta
     2641 tattggctga gcgcttgttc aatgatttca ggtttcaaac ctttttgctg catctgctgc
     2701 gctaatttct ttggtccttt gtcacttaag cgcatattcg tgcgcacata actttcccca
     2761 tagaccaaat catcgacaag gtccaattct tttaagcggg ctacgatttt atttcgatct
     2821 tcttgaagga tttccttttc ttttaagtat gttcggattt ctttttccga tcgcaactga
     2881 taacttaagt aattcattgc taggtgaaaa ccgaaatcag ctccgctact cttcttgatc
     2941 tcttggatag tttcttcgtc taattcccgt ccttttaata atcgatagcg tactaataca
     3001 tcttcagata cttgtaaagc ttctccttca gataaatcta cgcgatagaa agggcctttg
     3061 cctttactga ttctcgtaac ggttaacata aacatcctcc tcgaacatat attctctatc
     3121 ataccacaga aaatccccgt aatcaggtat tatatgttag aataaaccac agaaaaaaga
     3181 aaaagggaga tacttatggc agaaacaatt gtcaaaactg gacaaacggt tcctttgaaa
     3241 atcaagcgtt taggcataaa tggtgaagga atcggttatt tcaaacggtt gatcatattt
     3301 gttccatatg cactaccaaa agaagaagta ctagtgaaaa tcacgaaagc aactcctcgc
     3361 tatgcagaag gagaattaat aaaagtcaaa aaaacaagca aagatcgcgt agtggcacca
     3421 tgtcctgttt actatgaatg cggtggctgt caattacaac acttagctta tcaagctcaa
     3481 ttggatttta aaaaggatct gcttttgcaa gcattggaaa aattcaaacc agcaggcttt
     3541 cgatcttatc aacttttgcc gacgatcggg atggatgagc cttggcatta tagaaacaaa
     3601 gcacaatttc agttacgaaa aaataagcag actcaaaaaa ttgaagctgg tttatacgaa
     3661 gcaaactctc atcagttagt ccctataaca gactgcttgg ttcaagaacc tcgaacacaa
     3721 aaagtaatga atactgttgt tcaactattg aataaatttg atgcacccat ctatgatgaa
     3781 cggacaaaca gtggaatctt ccgcacaatc atggttagaa tcggtgttaa aactggggag
     3841 ttacaagttg tttttattac tcgcagtaaa aaattcccgc aaaaaaacgc aatgatccgt
     3901 gagatcaatg aacgactgcc cgaagttgtt tcaatcatgc aaaatgtcca ggcacaaaaa
     3961 acgtctctcg tgatggggga tgaaacgatc cacttatggg gaaaagaagc cattgaagaa
     4021 caaatcaatg aagtgacttt cgatctttct cctcgtgcct tctttcaact caatcctcag
     4081 cagaccgaat tgctttatgg cgaaggaatc aaagcattag atgtccagcc taatgaaacg
     4141 atcgtggatg cttattgcgg agttgggacg atcggcttaa gtgtagcaaa acaagccaaa
     4201 gaagtacggg gaatggatac cgttcctcaa gcaatcgctg atgcaaaaaa aaatgcggaa
     4261 agattaggat atcacaacac tcgatatgaa gtcgggacag cagagcaagt tctgcctaaa
     4321 tggttgaatg aaggttttca gcctgatggt atcatcgtag atccaccacg tacgggtttg
     4381 gataacgcat tgatccaatc attattgaaa taccctccaa aaaaacttgt ttatatctcc
     4441 tgtaatgttt ctacacttgc cagagatctg gttaatttaa gtaaaaagtt caagattgaa
     4501 tatttacagt ccgtcgacat gtttcctcag actgcacgtt gtgaagttgt cgtgaagatg
     4561 acgagactcg ctgactaaat gaaagaggtt gtgaaaaagt ctgccctgta tcaagtaata
     4621 agaacagcca aacaaaaata gctcttcatg ttttatgaat actgtacttg tgaccgaaga
     4681 gttaacagga ggacactatt tatttaaaaa aagggttgtg acatagtttt tgtcacagcc
     4741 ctttttgatt attattggat attcacgtct atcacttgat aaaatgcatt tgtcgtatcg
     4801 ctgatattcc atactgctaa aatcacatta tatccttttc gatcctgcgg aagtgtgatc
     4861 gtttggttga ctgtttttgg tggaacagtt gctttgtcgt ctatttttgt gatcaattca
     4921 aaatcactaa atttcaaagg ttgattagga ttccaagttg gtttggtaat gtagtaatcc
     4981 caagtacttg tccgatgttg tgctgttaaa ttccaagtaa tatttaatgt tcctggatta
     5041 accatgtttt tatgccatct gttagatgtc tgttcatcta aaggctcaaa gccgctgact
     5101 cctgcactag caatttttcc atcaatgaat gtatttttgg tagcttcgat actttgcggt
     5161 tcccattgtg ctcttccgac attttgattt aagtttcctg ctgctgatgt tcctaggtat
     5221 gcacggcttc ctggagaagt aatataacca tgcgcaaaac ctggaatgct aaaaccgaac
     5281 aaccctaaac taactaaccc aataaacaat aacgtactaa tctttttcat gtagaaacct
     5341 ccttcatttg ataaccacat tgtaaacgct ttattgtatt aacacaaaca ttatgctttt
     5401 ataattttat gttgaagtaa aaaaggtgaa aaaagtaaga tgcagattat aacagaagta
     5461 gacgagaaaa gtacaaatag aaaacatgag aatcaaatca tatgatcttt ttcaaaaaca
     5521 tcattcatat aaacaaatgt tcaacttttt aataacaaca attatcaatt tttttcttac
     5581 ttcacaaact aaatacaggt cttgctgtga gccttcatgg taaactacta ttatagaaag
     5641 gagttagtaa aatgaagatg ataaaagata ttcgtttttg ggtttgtgtt ataggaattg
     5701 tcatattagg ctttttatca gggttgctta gcggaaatcc cggagaatat tactattctt
     5761 tgcaacttcc accatttgca ccacctagct ggatatttgg gccgatgtgg accttactgt
     5821 atatattgat gggtatttct ctttatttgt tacttaatca caacaacaaa aaacagcgaa
     5881 acaatcttgt cggtcttttt gtgattcagt tcatttttaa ttttatatgg tctgctctat
     5941 tttttaa
//
LOCUS       DAXLPI010000132         5916 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXLPI010000132 DAXLPI010000000
VERSION     DAXLPI010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.135"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..1283)
                     /locus_tag="ACF3T5_002392"
     CDS             complement(<1..1283)
                     /locus_tag="ACF3T5_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632924.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKG"
     gene            complement(1581..2516)
                     /gene="murB"
                     /locus_tag="ACF3T5_002393"
     CDS             complement(1581..2516)
                     /gene="murB"
                     /locus_tag="ACF3T5_002393"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFD3632925.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2589..3509)
                     /locus_tag="ACF3T5_002394"
     CDS             complement(2589..3509)
                     /locus_tag="ACF3T5_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFD3632926.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3622..4374
                     /locus_tag="ACF3T5_002395"
     CDS             3622..4374
                     /locus_tag="ACF3T5_002395"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFD3632927.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5115..5648
                     /locus_tag="ACF3T5_002396"
     CDS             5115..5648
                     /locus_tag="ACF3T5_002396"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFD3632928.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1773 a         1140 c          991 g         2012 t
ORIGIN      
        1 cctttcactt gagaaataat gtaatcggta taagcagatc cttgcgaaaa atcaatacag
       61 acatgaatcg gtgcttcaaa atatgatacc ggacaaatac cgaccaataa aaagagataa
      121 tcataaaata agcgaatcat gaaacgttca tcttttccca agaaatagcg tttattctct
      181 tgtatgacct tttggattgc tgtactgaat attggatacg tctcgaagaa aaactgtagc
      241 tgctttttag acgtaaagct agtcatctca aagtcgaata tccaattttt tcgattgaca
      301 tgcatcatac ccatacgaag ttcttttcgt atatatagtc tttcttcttc agagatttca
      361 atcgaaaatg gtaattgatc aatcaattcc actgacattt tttgactaat catgtggata
      421 tcatgaaaat gagcttccgc aagtccaaat gcgccatcta attcaaattc ttcttccgtg
      481 atcaaaaaca acatgatata agaaatctca tcaatgatct gctctttagg gatatgaaga
      541 aaagaaatat gtgataaatc cttgatgatc ttcattcctt ctgagctctt aaagtattca
      601 tctgaaaaga aacgttttcc gattggttga ttcccatagg ccctcaacaa actaatagtg
      661 gttagcaaat tgaatttgat tcgttttgtt gcagacagtt ttaaattgaa cagatattca
      721 aaatattgca cgatactctt tgcttcattc atgacgcttt ctctaaatgg catagaaaaa
      781 ccattaaaga ccccgaaata taccccaaat aatacgttcc gtatgtttat ttcttcccca
      841 actaatatgt ttttctttaa ctttatttga tgctgttcaa gaaaagagac taattctctt
      901 ctttttacat aggctcttga tatgcttaaa aaatgattct ctgcaaattt ttcgatcgtt
      961 acttttgaat ccacgatctc cgttaacagt cgagcaattt ttgatcgttg aaagtagatt
     1021 cttcttaaat ttttgatatc cgtcaaattc agacgttcga catggatctc tgcttcttct
     1081 gaaatttgga tctctggctt tgttttgaat tcagtaaatt ctttatttaa ttctatgata
     1141 taatttcttg ttttaaattt agacaaacct aatgtttcat ataacttagg cagcactaca
     1201 taattctttt tctcaaattc taaatatcta gctagcagca gtttttctct atcttcctct
     1261 tctataaggt aatccaactt catatcctac acctcaaaaa atgcctcata ccaattactt
     1321 gatatgcaga tgaaagttca tcttttcacc tatactgatt ttatgatgga ttatgcttgt
     1381 tttttcatca gttattctca ttttttatct tttttttgag aatattgcaa aaaatggaaa
     1441 aatttatgag gaaatattct atattttttg ttttattgtg ttaaaatgta caagatttgc
     1501 gccatttgcg taaacaacca caaaaaagag caggacggcc accgtcctgc tcttttactt
     1561 attttaagtt ccattactta ttaattggct aaacttctgc ttgtttctga tattgcttgt
     1621 ttttctccaa tgatccgcac ttctgtttct aaagacacat caaatttttc tttgattact
     1681 tcttggatgt gcgcaatcaa ttcaatataa tctgtcgctg tcgcatgatc gatattgacg
     1741 atgaatccag catgtttttc agagacttgt gcaccgcccc atttcaggcc ttgcaatcct
     1801 gcttgttgga tcagctgacc tgtatagtgt cctactgggc gcttaaagac acttccgcat
     1861 gaagggtatt ctaatggctg ttttgattga cgcaattcgg tcaactcatc cattcttgct
     1921 ttgattgctt cataatcccc tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt
     1981 aattcttgga ctttactgtg acgataagaa aataccattt cgtcttttgt caaagttttc
     2041 aatgttccat cttcaagcaa aagatccact tcagcaaaaa catctttgat ctctccgcca
     2101 tacgcacctg cattcatata aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat
     2161 tcaaatccag tcaatgattc tgccaaagct gcataagtcg tatcaatcag tgaggcacct
     2221 gcttccgcaa tgaccatcgt tcctttgaca tgaatctgtt tcatttcagt cagcatgatg
     2281 actacaccgc gaatcccgcc atcttgaacg atcagattgc ttgcatttcc taataccatc
     2341 caaggtatat catggattcg gcaataatcg atgatttgct ttacttcttc ttgttttttc
     2401 gggaatgcaa ggacatcagc tggtccacct gttttcgtaa acgtatagtt catcaatggt
     2461 tcatcaaaca atatagtaat cgtggctaag ctttcaacta tttcttcttt attcactgag
     2521 gattttcctt tcttttctca aacgtcctca ttattctatc acgcttaata caatcttgcc
     2581 agtcttgttc aaagttgatg acggtagtga tctttaagca cttcctcaca taattcatga
     2641 cttcttacaa cttctttttg tttaagcaat tctgtttccg atttacctaa acggatctga
     2701 gaaaagaagg cctctaccat tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg
     2761 ccaaattctt tcactttttt tccatcagca tcttgaatga caagctgggt cagatcgttt
     2821 acttcataag taccattttt acttgttact tgatattttt ctatattagc gccacttgct
     2881 aaatccatcg ttaaaatagc cgtttgattt tgtgtttcta attgtaaaat ggcatattcc
     2941 aaaaattcct ttgtttctct tatctggtat tttgttctca aaatgggttc atctaataaa
     3001 tacacagctg tatcaataag atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct
     3061 tttgccgcta ttcgattttt ttgcaggtgg atcaaccgct tatctgggat acgtttcaat
     3121 tcttctacca taggagcaaa gcgccggttg aaaccgatca tcaaaattat ttggttctta
     3181 tcagctaatt cttgcaattc acgaacttca tttagatgga cactcagtgg tttgtcaata
     3241 taaacatgga taccattttc tagacatttt ttggctaatt cataatgtac ttttgttgct
     3301 gcgtggatca tacatgcttc gattttttct tctaaaagct catcgatcgt ttcatataga
     3361 tgttggaacc cgtactcttt ttgaattctt tcttttgttt ctttatttct tgttgcaaaa
     3421 taaaaatcaa gtgttccttg ttttttcgca taagttggta aatatgcttt ttctgcgata
     3481 ttccctacac cgattacacc aatcttcatc tactttcctc ctggatcatt tagttacttt
     3541 catcttacac cattttttac taaaatgagg tacaatagta tcgcaaaggt aaggaaatta
     3601 attcgaagga gtgtgctttt attgaaattt atctcttgga acgtaaacgg gcttcgtgcc
     3661 atcgtaaata aaaatttttt agaagttttt catgagttag acgccgattt tttctgtctg
     3721 caggaaacga aactccaagc aggacaaatt gatttagatc ttccaggtta ttatcaatat
     3781 tggaattacg cggaacgaaa aggctattcc ggcacagcga tttttgctaa aaaaccagca
     3841 ttgaatgcca cttacggaat gggtatcgat atccatgata cagaaggccg tctgattact
     3901 ttggaatatt ccgatttctt tcttgtaacc tgttacacgc caaattctca aagcgaatta
     3961 aaacgtttag attaccgatt agaatgggaa gaagcctttt ataattattt ggaaaatcta
     4021 aaaaaacaaa aaccagtcat cgtgtgtggg gatctaaatg tcgctcatca aaagattgat
     4081 ttgaaaaact ggaaaacgaa tcaaaaaaat gctggattca ctccagaaga aagagcggct
     4141 ctttctcgac tgttggataa cgggtttatc gatacattcc gttattttta tccaacccag
     4201 gaaggtgtct actcttggtg gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg
     4261 cggatcgatt actttttgac gagcaaagat ttagagccag gactagctga tgcaaagatc
     4321 catacggaca ttatgggcag tgatcattgt ccagtagaat tagatttgaa ataaagaggt
     4381 tgtgaaaaag tcgtttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
     4441 ttttgcataa atttggcata ataccgagaa actggctttt gaacactgtt tatcgagaga
     4501 ttgtgaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agttcctcat
     4561 atttttcgtt tggttgggct tattaccgca gatacaagct tttgaacacc gtttattaag
     4621 aggttgtgaa aaagctgcac tgcatcaagt aataagaaca gccaaacaaa aatagttcct
     4681 catatttttc gtttggttgg acttattacc gcagatgcaa gcttttgaac accgtttatt
     4741 gagaagttgt gaaatcagcg tcctgacctg agtattagag aaacaaatgg caaaatagtt
     4801 cctcatattt tgctattttt tgagctaata ccgagggtca gctgattgaa caccgtttag
     4861 tggagaagtt gtgaaaaagt cgcctacttt cgagtcgtaa gaaatttttt tgaaaatggt
     4921 tcctcacaac tttgcataaa tttgacttaa taccgagaag tcatgatccc catctaactc
     4981 caaatcacaa ggaatgagtt caaaaactag cttttctcaa caaatattac ataaaaaatt
     5041 ctatgttttt tgttaaattt atttgctttt ttcaaaagta tgttacagta atttcccaag
     5101 gaggttttac agaaatgaat tttgttgcca tggattttga aactgccaat catcagcctt
     5161 atagtgcttg ttctcttgcg ctagtcatgg taaaaaatag ccagatcgtt gatgaattct
     5221 atacattgat acagcctgag actccttttt tctggcgaaa tgtccaaatt cacggaatcc
     5281 atcaagaaga cgtacgcaat gcgccgaaat tcccagaagt ctggcaaact atccaaccat
     5341 attttcaaaa aaatcgttta gtcgttgcac ataatgctgc ttttgataca aaggtcttag
     5401 cgggatgctt agattattat catcttgaac agcctaatta tctttcttta tgtacagtga
     5461 aaagtagccg tcgcctgttt cctgaatttc caaatcatcg attgaacact gtgtgcgaaa
     5521 atttgaacat ccaattagat catcaccatg atgcattgga agatagtcgt gcatgtgcgg
     5581 aaatcctgct ttatcaagaa aaaaaattcg ggacagatcc attgaaaaaa cttgtgacta
     5641 taaagtaaaa gaatacttat gaaacacaca aagaggtcgt gagcctgccg tttcactccg
     5701 agtcacaagg agcaatttca aaaaatagct tctcatgttt attaaatatt gtgactggtg
     5761 accgaggagc tggcaggaga acaccgttta ttggagaggt tgtgaaaaag ctgttctgca
     5821 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcattt ggttggactt
     5881 attaccgcag atgcaagctt ttggacaccg tttacc
//
LOCUS       DAXLPI010000133         5912 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXLPI010000133 DAXLPI010000000
VERSION     DAXLPI010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.136"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(99..1229)
                     /locus_tag="ACF3T5_002397"
     CDS             complement(99..1229)
                     /locus_tag="ACF3T5_002397"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFD3632929.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACF3T5_002398"
     CDS             complement(1878..3080)
                     /locus_tag="ACF3T5_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3632930.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACF3T5_002399"
     CDS             complement(3140..3343)
                     /locus_tag="ACF3T5_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFD3632931.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACF3T5_002400"
     CDS             complement(3485..4552)
                     /locus_tag="ACF3T5_002400"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFD3632932.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACF3T5_002401"
     CDS             complement(4610..4717)
                     /locus_tag="ACF3T5_002401"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632933.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACF3T5_002402"
     CDS             4880..5692
                     /locus_tag="ACF3T5_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632934.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1748 a         1219 c          949 g         1996 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgggctct ataatttatg
     5881 ggactgatga atcagaattg attcttcagc cc
//
LOCUS       DAXLPI010000134         5843 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXLPI010000134 DAXLPI010000000
VERSION     DAXLPI010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.138"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            254..751
                     /locus_tag="ACF3T5_002403"
     CDS             254..751
                     /locus_tag="ACF3T5_002403"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HFD3632935.1"
                     /translation="MLFLRDGEIKATLTTMMNKLAFSHKLILEPLFKSVSQIDEESDR
                     ERMDAIDKLMEQLLEERNTLIALMSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTN
                     SASGVLRANEIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLK
                     EKVVR"
     gene            752..1168
                     /locus_tag="ACF3T5_002404"
     CDS             752..1168
                     /locus_tag="ACF3T5_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFD3632936.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            1188..2732
                     /locus_tag="ACF3T5_002405"
     CDS             1188..2732
                     /locus_tag="ACF3T5_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3632937.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            2875..3099
                     /locus_tag="ACF3T5_002406"
     CDS             2875..3099
                     /locus_tag="ACF3T5_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3632938.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            3114..3983
                     /locus_tag="ACF3T5_002407"
     CDS             3114..3983
                     /locus_tag="ACF3T5_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFD3632939.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDWINGGGWLFINGYHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            3964..4698
                     /locus_tag="ACF3T5_002408"
     CDS             3964..4698
                     /locus_tag="ACF3T5_002408"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3632940.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            4731..5639
                     /locus_tag="ACF3T5_002409"
     CDS             4731..5639
                     /locus_tag="ACF3T5_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFD3632941.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            5636..>5843
                     /locus_tag="ACF3T5_002410"
     CDS             5636..>5843
                     /locus_tag="ACF3T5_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632942.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDN"
BASE COUNT         2066 a          915 c         1283 g         1579 t
ORIGIN      
        1 aagtattctg cttcatcacc cattaggtga tgaaaaaaga acccctttct gatttagcgg
       61 acaaatactt tgacctaatc tcagaagata taaaagaaaa aataataaaa tatccgattt
      121 atactttgta taaagaacta aagtagttag caaatcattt acagcaaatc actatgttcc
      181 acataccatt ttacatactt accttatcca cgacacatgt tgagatagta tcgaagataa
      241 agaaagttgc tccatgttgt ttttgcgaga tggggaaatc aaagccacat tgacaaccat
      301 gatgaataag cttgctttca gtcataagct aatcctagaa ccacttttca agtcagttag
      361 ccaaatcgat gaagaaagcg accgtgaaag aatggacgct attgataagc taatggagca
      421 actattggaa gaacgcaata cccttattgc actgatgtcc aaaggtttcc tagaacctgc
      481 tctttttaat caggaacgaa atgttttaga tagtgagatt aaaaatctta ctaccgaaaa
      541 aacaaacctg gtaacgaact ctgcgagtgg ggttttgcga gcaaacgaga taaaggacct
      601 cattaattat gtgtcagcag ataattttaa tggtgactac acggaagaac tatttgaaga
      661 atttgtagtg aacatcattg taaattccag ggatgagctg acattcaatt tgaaatgcgg
      721 tctttccctg aaagaaaagg tggtgagata aatggcatat attccatatg gatacaaaat
      781 tcaagatgga gtggttactg tcgatgaaaa ggcagcaggt caagtaaagg tattctttga
      841 gaaatacata tcaggactat cccttacagt ggctggcgaa caggcaggta ttgaaaagac
      901 acattctgtg atggggcgca ttttgaaaaa cgtcaactac cttggaaatg atacgtatcc
      961 agcaattatt gataaagaga tatttgataa agctgaagaa gttagagata aacgtgcaaa
     1021 ggatttagga cgagtggtag agcttgccgc tttcacctct ccccctccca aagaacgatt
     1081 caaaatgaaa aaggcagata ataagatgcc agtggatcct tttgaacgag cagaatactt
     1141 atacagtctg atagaaagcg aggaataaag tgacagagaa aaatataatg gttattcctg
     1201 ctcgtaaaag agtaggaagt acagccgcaa aagaaaagat aaagaaactt cgtgttgctg
     1261 cctattgccg tgtttctaca gaaacagaag aacaaaattc tagctatgag gttcaggtct
     1321 cacactatac agagtttata aagaaaaata atgaatggga gtttgctggc atctttgcag
     1381 atgatggtat ctctggtacg aacactaaaa agcgtgacga atttaatcgc atgattgcag
     1441 agtgtatgga cggtaacatc gacatggtta ttacaaaatc catcagccga tttgcgcgta
     1501 acaccctaga ctgccttcaa tacattagac agctcaagga taagaacata tcagtttatt
     1561 ttgagaaaga gaacatcaac accacggatg ccaaaggtga ggttttgctg actattatgg
     1621 catctttggc acagcaggaa agccagagcc tttcacaaaa cgttaagctt gggctacagt
     1681 accgatacca acaaggaaag gtgcaggtca accataagcg atttatgggc tacacgaaag
     1741 atgaagatgg aaatttaact attgtccccg aagaagctga gattatcaaa cgcatctacc
     1801 gagaatacct tgagggtcag agtctagtgg gcattggtcg agcccttgaa aaggatggta
     1861 ttttaacagc agcgggaaaa ccaagatggc gaccagaatc agttaagaag atacttcaaa
     1921 acgaaaaata catcggagat gcccttctgc aaaagactgt cacagtagat tttctgacca
     1981 agaaacgagt gaaaaatgaa gggcatgttc cccaatatta tgttgaaaat agccatgaag
     2041 cgattattcc taaagagtta tttttgcagg cgcaagaaga aattcatcgg agaagcaata
     2101 tctacacagg agaaggcaag aacaaaagaa tttacagtag caagtacgct ttaagtgcca
     2161 tcaccttctg tggagattgt ggcgatattt atagacgggt ctattggaat atccacggaa
     2221 gaaaagaatt tgtctggcga tgcgtgacta gaatcgagca aggtcctgaa gtttgtaaga
     2281 accgaaccgt aaaagaagat gaactttatg gtgcagtaat gactgcgatt aataagctac
     2341 ttgcaggtgg gaacaatatg ataaagacac tggaagagaa tattcatgct gtgattggcg
     2401 aaaccacaga ataccaaatt tcagagatta acaactcact agaggaaaaa caaaaagaac
     2461 taatcaagct ggcgaacaag ggtcaagact atgaccactt agcagatgag attgatgagc
     2521 tgagagacaa gcgacagatc cttttagtag aagatgcctc cctcagtggc gagaacgagc
     2581 gaatcaatga gctgattgaa tttatccgca agaacaaatt ccgcacctta gaatatgatg
     2641 ataagcttgt aaggaagata atccagagcg ttacagtcta tgaaggccac ttcgtcatag
     2701 ccttcaaatc tggcatcgaa atggaaatat gaaaaccaga agtaaatagc ccatgactct
     2761 acagtagagt tgtgggtttt cttttgcttg ctgattataa taatatttta ataactattg
     2821 tgtttatcaa ccaaatggtt tataatattc ttagcgtaaa cttggaggtg ctgtatgact
     2881 acggcagaaa tgattaaaga actgtgtgag caaatgaata taagtgtttc cgaacttgct
     2941 agacgtattg gccagactcc acagaatttc aataaaaaat tacaacgaga aacagtaacc
     3001 ttggatgagt tgaaggccat tgctgatgtg ctaggtgtca agtttgtgca ggcatttatt
     3061 ttaccagatg gagaagaaat aaaaatatct aacgaataaa ggaggatgtt tatatggtag
     3121 gtaatgttat tcaaatagta acagagaaat tatcatcttt gccttttata gaaggcatcg
     3181 tattaggggg ctcacgtgca agaggcaccc atacagaaaa ttctgatata gatatcggaa
     3241 tctattacaa ttcagactca tttgacctga cagcgattaa tcaaattgct acagaattgg
     3301 atgataaaaa tagaaacaac cttgttgtgc ctcccggagc gtggggtgat tggattaatg
     3361 gcggcggatg gttatttata aatgggtatc atgttgactt gattttacgt gatataaaac
     3421 gggtagaaca aataatcaaa gatacggagc aaggaattgt tactgccaat tatcagaccg
     3481 ggcatcccca tggttatata agtgcaatgt atcgtggaga attagcgatt agtaaaatac
     3541 tatatgctaa gaacgaaagc ttatgcgaat taaaaaagca ggcagaaatt taccctactg
     3601 ctctaaagaa aagcttgatg aactttttta tatttgaagc tgaattctct ttaatgtttg
     3661 taaaagcaaa tgcgggggta gaggataaat attatattgc aggccatgtt tttcgtatta
     3721 tttcatgctt aaatcaagta ctatttgcat gtaataatgc ttattgcatt aacgaaaaga
     3781 aagctataaa actgcttgaa acttttgaat ataaacctga aaaatatgcc gaaagagtta
     3841 atcatatttt tgaagtactc ggtctttctc tttttgaatg ctacgatatg accgagaaac
     3901 tttataaaga agtgaatgaa attgtatcgg agataaataa ctttttaaac gaggagagtt
     3961 cagatgaaag aaaacaaata tgatgataat atattttttc aaaaatacag tcaaatgagt
     4021 cgctcgcaga aaggactggc tggtgcggga gaatgggaga ctttgaaaaa gatgctacct
     4081 gattttaagg gtaagcgtgt gcttgattta ggatgcggct atggatggca ctgtatatat
     4141 gcgatggaaa acggtgcttc ctctgtagta ggtgttgata tttctcataa aatgctcgaa
     4201 gtagcaaaag gaaaaaccca ttttccacag attgaatatg aatgctgtgc catagaagat
     4261 gtggatttcc cagaggagag ctttgatgta atactaagtt cgcttgcgtt tcattatgta
     4321 gcagactatg agaatttaat aaaaaagata tataggatgc tgaaggctgg tggcaattta
     4381 gtttttacag ttgaacatcc tgtttttact gctcatggaa cacaagactg gtattataac
     4441 gaaaaaggag aaatactgca tttcccggtg gacaattatt attatgaggg caaacggaca
     4501 gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa cactgaccac atatctaaat
     4561 acactgcttt caaatagttt tataataaat cagattgtgg agccacagcc gccagagaac
     4621 atgatggata ttccggggat ggcggatgaa atgcgacgcc caatgatgct gattgtatcg
     4681 gcaaaaaaga agatgtaata atatagaaaa aataaacgag gagtatgtaa atgagatcag
     4741 aaaaagaaat gatggattta gtactttctt tagcagaaca ggatgaacgt attcgaattg
     4801 tgacccttga ggggtcacgc gcaaatatta atatacctaa agatgaattt caggattatg
     4861 atattacata ttttgtaagt gatatagaac cgtttatatc taatgatgac tggcttaatc
     4921 aatttgggaa tataataatg atgcaaaagc cggaggatat ggaattattc ccacctgaag
     4981 aaaagggatt ttcctatctt atgctatttg atgattacaa taaaattgat cttaccttat
     5041 tgcccttgga agagttagat aattacctaa agggcgataa attaataaag gttctaattg
     5101 ataaagattg tagaattaaa agggacatag ttccgactga tatagattat catgtaagaa
     5161 agccaagcgc aagggagtat gatgattgct gcaatgaatt ttggaatgta acaccttatg
     5221 ttattaaagg attgtgccgt aaggaaattt tatttgctat tgatcatttt aatcagattg
     5281 ttcgccatga gctgctgaga atgatatcat ggaaggtcgg catcgaaaca ggctttaaat
     5341 taagtgtagg caagaactat aagtttattg aaaggtatat atccgaggat ttgtgggaga
     5401 aacttttgtc cacctaccgg atggattcct atgaaaacat atgggaagca ttatttctat
     5461 gccatcaatt gttcagggcg gtatccggtg aggtggcgga aaggcttcat tatgcctatc
     5521 cggagtatga taggaatata acaaaatata ccagggacat gtataaaaaa tacactggta
     5581 aaaccggctg cctggatagc acatatgccg ctgatataga agagaggcgg gaacagtgat
     5641 tacagaaatg aaagcagggc acctgaaaga tatcgataaa cccagcgaac catttgaggt
     5701 gataggtaag attataccga ggtatgaaaa cgagaattgg acctttacag aattactcta
     5761 tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat gaagaggatg aggaggcaga
     5821 ttgccttgaa tatattgaca ata
//
LOCUS       DAXLPI010000135         5840 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXLPI010000135 DAXLPI010000000
VERSION     DAXLPI010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.139"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            146..1462
                     /locus_tag="ACF3T5_002411"
     CDS             146..1462
                     /locus_tag="ACF3T5_002411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3632943.1"
                     /translation="MLVNEVLKTNFLETIKKYYDYEDIYEIAQFETSNANDFIKKQRT
                     EQGKNALDIWKKRYDSQFSAIQDYLKNNEEDFNQDVEKFVESVKQFAKDSTTSNSTSK
                     YQLIKESNLEEISTKNLETHLYKLLDLINSPDLWELLSNSVEKRRVFVETYSEVRNLF
                     VQKQQNNKVREKVNDILGSVKKIVQSKTGITPVVDCEFSKIIQKHQIEKEINNFMQKI
                     VCNTEIKKEKIYGYQIIVKLSPFINATQFRKDRTSEAVQDDLIVPYKSGQYITFLRNL
                     KKRSFFNPSILEEYLLHLEVNLLDSDGVPASGGQAVGFALMMRLEEAKTKPIILIDEP
                     EASLDNEYIRKELRDAIRNLSASSTVFVVTHNSTLGALLEPDYLIVTTKDKEKGYRVL
                     TGEFSSHLISTKSDTTESSYEKFVEAMEAGIETYKKKGEIYESLNS"
     gene            1443..1727
                     /locus_tag="ACF3T5_002412"
     CDS             1443..1727
                     /locus_tag="ACF3T5_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632944.1"
                     /translation="MKVLIVNSGKSKFVIGENNVEPENLSRDDLLMILNNIYESEENV
                     VIPSIEELDEIRNPIEKEIVQQIIQKISDFSNNVENIRQEVQAQFPVIED"
     gene            1765..2088
                     /locus_tag="ACF3T5_002413"
     CDS             1765..2088
                     /locus_tag="ACF3T5_002413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632945.1"
                     /translation="MVGIDNDSNFKSYEFEKTYDFSVEIPIVDEDTGEYTREIELVAG
                     QITFFGDADWDSTEKEYVYNFSWEESTRTFSHYGNAPDKEDEFIKDFKEYLLQQGVER
                     YNIGW"
     gene            2139..2363
                     /locus_tag="ACF3T5_002414"
     CDS             2139..2363
                     /locus_tag="ACF3T5_002414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030127 - COPII vesicle coat [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec23/Sec24 zinc finger-containing protein"
                     /protein_id="HFD3632946.1"
                     /translation="MTERFPDTDWWCDRCGAHLNSQNNFDDYKYTWKCTECGFKNSIS
                     KDNIYESHEEYYNTGDDDSGLLRSIFKKLF"
     gene            complement(2750..3370)
                     /locus_tag="ACF3T5_002415"
     CDS             complement(2750..3370)
                     /locus_tag="ACF3T5_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFD3632947.1"
                     /translation="MATKVFISFRFSDGKEIKDELVDLFDESTEVINRSEDVDRSQMS
                     KDTIQEYLYEKLKDTSVTIVLLTPEAVSYRKNWIGNYDDWIYDELRYSLEDRNNNRAN
                     GVVAVYTDDAKDMILESSSHYCAHCQETKSCRTLKSFDNLARKNMLNIKQSYKKNPCN
                     DLYDDSHDSYISLVSLEEFKQDYTKYIDNAKDKRDRLHEFNIYKRM"
     gene            complement(3370..4782)
                     /locus_tag="ACF3T5_002416"
     CDS             complement(3370..4782)
                     /locus_tag="ACF3T5_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFD3632948.1"
                     /translation="MRQELFIKKFSKAIQEGNAAVFAGAGTSVDAGFVNWKNLVAPFA
                     NEIDLDIEKETDLVGLTQYYINKKRNRGSVNQEIIDQFSSQYAETEVMNLLTRLPIST
                     YWTTNYDKVIENGLKNNNRRGDIKRKTDDLAISIRDSDAIVYKMHGDVDSPNDAVISK
                     DDYERYSEKNSLFVTALRGDLVSKTFLFVGFGFEDPNLESILGKIKNLLGERTRDHYC
                     IQKKVLEQDYKNREEYSYAKIKQELKIEDLKRYGIDTVLVDDYSEIPKLILKIEEEYF
                     KNTIFISGSISDYNENWSQEKVNQFCYNLSRSLVSKNYKIISGFGLGVGSSVINGALD
                     EIYNTKYRHVSEHLGLFPFPQHDAGEKSLAKRWTENREQMISEAGICIFIFGNKLVNG
                     EVVLASGMMEEFRIAKERGKVIIPIGSTGFVAKEIFDEMKESGDYSYINDYWNDLENE
                     EDVIKVFNTINEIIEGVRFR"
     gene            complement(4812..>5840)
                     /locus_tag="ACF3T5_002417"
     CDS             complement(4812..>5840)
                     /locus_tag="ACF3T5_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFD3632949.1"
                     /translation="YMFPDKILLIERERNYWYGKRIIRKNLSKIRCKGMSLETDSFIL
                     STSKHIRKIKRESLASNEEMVKKVNTARTISLEKLKAESLRSKRKLNYIQEVEPPYLR
                     NYRNRFFKTHDLHERLEIIRELSKYDSKEVREFFYAINGHIRNQSLKIEVQSYFQSLS
                     LPFRLSRKKKGKKNFIDNEIVKNKKGPDELKKRLYINDLEKIKRFDIFVSHNSRDEEK
                     IIEFYKKFNKKGQVVYIDWINDKYDLRREWCNASTVDIIKERIKQSDVFVLYFSGETL
                     NSQWCTWELGYADALGKKICVYFSEDINRKGIPKFYVAYPELQMSKNIYVKIRGNEDI
                     LLKEWKKL"
BASE COUNT         2023 a          934 c          814 g         2069 t
ORIGIN      
        1 aatacgtact tacaaattga caattgctcg tttcaagaaa taaagaattg tttaatggat
       61 aaaacaaaag ttggtataaa ccttagatat ttgagggacg taatcgatat tgaaaatcac
      121 actgtttcaa ctggattaaa tttaattgtt ggtaaacgag gtactgaaaa ccaatttttt
      181 agaaacgata aaaaaatatt atgattatga agatatttat gagatagctc aatttgaaac
      241 atcaaatgcc aatgatttta taaaaaaaca aagaacagaa caagggaaaa atgcgttaga
      301 tatatggaaa aaacggtatg actcgcagtt ttctgctatt caagactatc taaaaaataa
      361 tgaagaagat tttaaccaag acgtagaaaa atttgttgaa agtgtgaagc aattcgccaa
      421 agattcgact acaagtaact caacttccaa atatcaactt attaaagaat cgaatcttga
      481 agaaatttcc acaaaaaact tagaaacaca cttgtacaaa ttacttgatt taattaatag
      541 tccagattta tgggagctgt taagtaattc agttgaaaaa aggagagttt ttgttgagac
      601 atatagtgaa gtaaggaatt tgtttgttca gaaacaacag aataacaaag ttcgagaaaa
      661 agtaaatgac atactaggtt ctgtaaaaaa aatagttcaa tctaaaactg ggattacccc
      721 tgttgttgat tgtgagtttt caaagataat tcaaaaacat cagattgaaa aagaaattaa
      781 taatttcatg caaaaaatag tctgtaatac tgaaataaaa aaagaaaaaa tttatggata
      841 ccaaattatt gtgaagctat ctccgtttat aaatgctact cagtttagaa aagatcgtac
      901 aagtgaagca gttcaagatg atttaattgt tccctataaa agtgggcagt atattacatt
      961 tttaaggaat ttgaaaaaaa gaagtttctt taatccttct attttagaag aatatctatt
     1021 gcatttagaa gttaacctgc tggattcaga tggtgtacca gcatctgggg gacaggctgt
     1081 gggatttgct ctaatgatga gattagaaga agcaaaaaca aaaccaataa ttttaatcga
     1141 tgaacccgaa gcgtcacttg ataatgaata cattcggaag gaattaagag atgctataag
     1201 gaatttgtct gcaagcagca ctgtttttgt ggttacacat aattcaacat taggagcatt
     1261 actggaacca gattatctaa tagtgactac gaaagataaa gaaaagggat atcgcgtact
     1321 taccggtgaa ttttcttctc atcttatctc tacaaaatca gatacaactg aaagtagcta
     1381 tgaaaaattt gtagaagcaa tggaagcagg aatagaaaca tataaaaaga aaggagaaat
     1441 atatgaaagt cttaatagtt aattcaggaa aatcaaagtt tgttataggt gagaataatg
     1501 tggagccaga aaacctttct agagatgatt tattaatgat actaaataat atttatgaat
     1561 ctgaagaaaa cgtggtgatt cctagtattg aagagttaga tgagattaga aatcccattg
     1621 aaaaagagat tgttcaacaa attattcaaa aaatttcaga tttttcaaat aacgttgaaa
     1681 atattcgcca agaagtgcaa gcgcagtttc ctgttataga agattaaaat ttatatgcta
     1741 ataaaaaata aaggagtgag gaaaatggta ggaatagata atgatagtaa tttcaagtca
     1801 tatgaattcg aaaaaacata cgattttagc gtagagattc caattgtaga tgaagatacg
     1861 ggggaatata ctagagaaat agaattggta gctggacaaa taacattttt tggagatgca
     1921 gactgggatt caacagagaa agaatatgtt tataattttt cttgggaaga gtctactaga
     1981 acatttagtc attatggtaa tgcacctgat aaggaagatg agtttattaa agatttcaaa
     2041 gagtatttat tacaacaagg agttgagagg tataatattg gatggtaaat gttgaaatta
     2101 attataaaaa tttttaaata tgtagggaga gatatactat gacagaaaga ttcccagata
     2161 ccgattggtg gtgtgaccgt tgcggggcac atctgaattc tcaaaataat tttgatgact
     2221 ataaatatac atggaaatgt actgagtgcg gttttaaaaa tagtatttca aaagataata
     2281 tatatgagag ccatgaggaa tattacaata caggtgatga tgattctggt ttactaagat
     2341 caatttttaa gaaattgttt tagtgctgaa ttacttacag attttctgag aatgccttga
     2401 aacgttaatc ttccggtagt taaaggagag gtaatcactt ttcttttttt tacttcattg
     2461 ttccataact ttatccaagg tgattaattt gacatagaac ttgtgatgga tttatggaca
     2521 cgtgtttcaa ttcttttaga gaaaaatcat accaaaatag cagtgtccag aaatgaacta
     2581 ttttatggac atgagataaa aaagaaaaac agccatttcc gtttttcttt tttatctttg
     2641 tgtctaaata tttgacatta gtccaaacaa aagcacttta taaatcttgt acagaaaagt
     2701 gttgcctttt catttattct aaagacaatt tatgctaaat caatgctctt cacattcttt
     2761 tatatatatt aaattcatgt agcctatctc ttttatcttt tgcattatca atatatttag
     2821 tataatcctg tttaaactcc tccaaactta ctaaagaaat ataagagtca tgactatcat
     2881 cataaagatc attacatgga tttttcttat agctttgctt tatattcaac atattctttc
     2941 gtgctaaatt gtcaaaagat ttcaaagttc tacatgattt agtttcttga caatgtgcac
     3001 aatagtgtga actactttct agtatcatat ctttagcatc atctgtatat actgctacta
     3061 cgccatttgc tctgttgtta tttctatctt ccaaggagta tctaagttca tcataaatcc
     3121 aatcatcgta atttccaatc caattctttc tataagaaac ggcctctgga gttaataata
     3181 caattgtaac cgatgtatct tttaacttct catatagata ttcctgaata gtgtccttac
     3241 tcatttgact tctatcaaca tcttctgatc tattaattac ctcagtagat tcatcaaata
     3301 aatcaactag ttcatcttta atctcttttc catcactaaa acgaaatgaa ataaatactt
     3361 ttgttgccat tatctaaatc taactccttc aataatttca tttattgtat taaaaacttt
     3421 tataacatct tcttcgttct ctaaatcatt ccaataatcg tttatatatg aatagtcccc
     3481 tgactctttc atttcatcaa aaatttcttt tgcaacaaac cccgtagaac caattggaat
     3541 tattactttt cctctttctt tagcaatccg aaattcctcc atcattccag atgcaagtac
     3601 tacctcacca ttaactaatt tattaccaaa tataaaaata cagatcccag cctcactgat
     3661 catttgttca cgattttcag tccaacgttt agctagagac ttttccccag catcatgttg
     3721 aggaaaagga aataatccaa gatgctcact gacatgccta tacttagtgt tataaatttc
     3781 atcaagagct ccattaataa cactactacc tactccaagt ccaaaccctg atataatttt
     3841 ataatttttt gaaacaagtg aacgcgataa attataacag aattgattta ccttttcttg
     3901 actccaattt tcattgtaat cggagatact acctgaaata aaaattgtat ttttaaaata
     3961 ctcttcctct atttttaata tcaacttagg tatttcagaa taatcatcaa ctagaactgt
     4021 atctataccg taccttttca aatcttctat ttttaattct tgtttgattt tagcatagct
     4081 atattcctct cgatttttat aatcttgctc taataccttc ttttgaatac aataatgatc
     4141 tctagtacgc tccccaagta aattttttat ctttcccaag atggattcta aattaggatc
     4201 ttcaaaacca aaccccacaa acaaaaatgt tttggaaact aagtcacctc gtaaagctgt
     4261 tacaaatagg gagtttttct cgctatatct ttcataatca tcttttgaaa taactgcatc
     4321 attaggtgaa tctacatcac cgtgcatctt ataaacaatc gcatcgctat cacgaatact
     4381 gattgctaaa tcatctgtct tcctttttat atctcctctg cgattattat tttttaagcc
     4441 attttcaatt accttatcat aattagttgt ccaatatgta gaaattggaa gtcgtgttaa
     4501 cagattcata acttctgttt ctgcatattg actagaaaat tggtcaatta tttcttgatt
     4561 caccgaacct ctatttcttt ttttattgat ataatattga gtcagtccta caagatccgt
     4621 ttctttttca atatctaaat caatttcatt cgcaaacggt gctacaaggt ttttccagtt
     4681 aacaaatcct gcatcgaccg atgttcctgc accagcaaac actgctgcat ttccttcttg
     4741 tatagctttc gaaaattttt ttataaataa ttcttgtctc atattaccct catttctaat
     4801 ttattataag cctataattt tttccactct ttaagtaaaa tatcttcgtt ccctctaatt
     4861 ttgacatata tattttttga catttgcaat tcaggataag ctacatagaa ttttggaata
     4921 ccttttctat ttatatcttc tgagaagtaa acacatatct tcttccctaa tgcgtcagca
     4981 tatcccaatt cccaagtaca ccattgggaa ttcaaagttt ctcctgaaaa gtataataca
     5041 aaaacatctg attgttttat acgctccttg ataatatcaa ctgtggaggc attgcaccat
     5101 tcacgcctta aatcgtattt atcatttatc caatctatat aaactacctg tcctttttta
     5161 ttgaactttt tatagaactc tataattttt tcttcgtctc tagaattatg cgatacgaat
     5221 atatcaaatc tttttatctt ttctaaatca ttaatatata atctcttttt caattcatct
     5281 ggtccttttt tattttttac aatttcgtta tcaataaaat tcttcttacc tttctttttt
     5341 ctgctcaacc taaaaggtaa agataaactc tgaaaatatg attgaacttc tatttttaat
     5401 gattgatttc taatatgccc atttattgca tagaaaaact cccgaacttc ttttgaatca
     5461 tacttagaaa gttctcttat aatttccaat cgttcatgta aatcatgagt tttaaagaat
     5521 ctatttcgat aattccttaa atatggaggt tcaacttcct gaatataatt taattttctc
     5581 ttgcttctca aggactctgc ttttaatttt tctaaagata ttgttcgggc agtatttact
     5641 ttttttacca tctcttcgtt actagctaga ctctctcttt ttatcttcct aatatgtttt
     5701 gaagttgaaa gtataaaact atcagtttct aaagacatac ctttacatcg aattttggaa
     5761 agattctttc taataatacg ttttccatac caataatttc tttccctctc tatcagcaat
     5821 attttatctg gaaacatata
//
LOCUS       DAXLPI010000136         5689 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXLPI010000136 DAXLPI010000000
VERSION     DAXLPI010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.140"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(488..1297)
                     /locus_tag="ACF3T5_002418"
     CDS             complement(488..1297)
                     /locus_tag="ACF3T5_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFD3632950.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="ACF3T5_002419"
     CDS             complement(1297..2043)
                     /locus_tag="ACF3T5_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFD3632951.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="ACF3T5_002420"
     CDS             complement(2061..2528)
                     /locus_tag="ACF3T5_002420"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3632952.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="ACF3T5_002421"
     CDS             complement(2528..2938)
                     /locus_tag="ACF3T5_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFD3632953.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="ACF3T5_002422"
     CDS             complement(2949..3962)
                     /locus_tag="ACF3T5_002422"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFD3632954.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="ACF3T5_002423"
     CDS             4179..4913
                     /locus_tag="ACF3T5_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3632955.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="ACF3T5_002424"
     CDS             4897..5607
                     /locus_tag="ACF3T5_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3632956.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          942 g         1720 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgcttt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAXLPI010000137         5629 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXLPI010000137 DAXLPI010000000
VERSION     DAXLPI010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.141"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..694
                     /locus_tag="ACF3T5_002425"
     CDS             <1..694
                     /locus_tag="ACF3T5_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFD3632957.1"
                     /translation="TTLREMKVTKETLTTVLNEIFSTPTKEGIQFEIQGMKETREADY
                     YPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTIPLESYPTEQII
                     AEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTRRGKTDPLKIYY
                     EKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVIHVEEKERKLAY
                     QRHQQMNREMER"
     gene            697..1182
                     /gene="ssb"
                     /locus_tag="ACF3T5_002426"
                     /pseudo
     CDS             697..1182
                     /gene="ssb"
                     /locus_tag="ACF3T5_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            1227..1460
                     /locus_tag="ACF3T5_002427"
     CDS             1227..1460
                     /locus_tag="ACF3T5_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632958.1"
                     /translation="MDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVFVYLVCSYL
                     LKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(1619..1741)
                     /locus_tag="ACF3T5_002428"
     CDS             complement(1619..1741)
                     /locus_tag="ACF3T5_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HFD3632959.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            1896..2155
                     /locus_tag="ACF3T5_002429"
                     /pseudo
     CDS             1896..2155
                     /locus_tag="ACF3T5_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2603..2809
                     /locus_tag="ACF3T5_002430"
     CDS             2603..2809
                     /locus_tag="ACF3T5_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFD3632960.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            2809..3060
                     /locus_tag="ACF3T5_002431"
     CDS             2809..3060
                     /locus_tag="ACF3T5_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632961.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            3076..3513
                     /locus_tag="ACF3T5_002432"
     CDS             3076..3513
                     /locus_tag="ACF3T5_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632962.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            3506..4201
                     /locus_tag="ACF3T5_002433"
     CDS             3506..4201
                     /locus_tag="ACF3T5_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632963.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            4366..4566
                     /locus_tag="ACF3T5_002434"
     CDS             4366..4566
                     /locus_tag="ACF3T5_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632964.1"
                     /translation="MNKIIPLLLVLFVGEILMRLSAAVHLNLAVFLVILMFCLIFWRF
                     VLALVSLLFKMIAAIFFIGLFR"
     gene            4582..4761
                     /locus_tag="ACF3T5_002435"
                     /pseudo
     CDS             4582..4761
                     /locus_tag="ACF3T5_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4823..5007
                     /locus_tag="ACF3T5_002436"
                     /pseudo
     CDS             4823..5007
                     /locus_tag="ACF3T5_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            5041..5472
                     /locus_tag="ACF3T5_002437"
     CDS             5041..5472
                     /locus_tag="ACF3T5_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFD3632965.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         1995 a          950 c         1149 g         1535 t
ORIGIN      
        1 tacgacttta cgggaaatga aagtaaccaa agaaacatta acaacggttc taaacgaaat
       61 tttttcaacg ccgacgaaag aaggcattca atttgagatt caaggaatga aagaaacgag
      121 agaagccgat tactatccag gatttagctt acgtgtgctg gcacatttgg aaaacatgcg
      181 gccagatttt aaggtagatg tgactacagg tgactcgatc tatcctgcaa cgatcactca
      241 cagtcataaa ttgatgtttg aggatcgaac gattcctttg gaatcctatc ctacagaaca
      301 aattattgct gaaaaattaa gtgcaacctt tgatttttta acagataatt cgagaggaaa
      361 agatttttat gatctctata ctattcctaa aatggagaag atcgatgaga agaccttgaa
      421 agatagtgtg agaaatacat ttacacgtcg aggaaaaact gatccgttaa agatatacta
      481 tgaaaaggat atggatgtac tgaaaaatag tcacgagcta aaagatacct ggaagaaata
      541 tcaaaaaagc aatccttatg ctgcgtcgat cacgtacgat gagacaatgg attcgattag
      601 taatttgatg gaaaaagtga ttcacgtcga agaaaaagag cgaaaactag cttatcaaag
      661 acaccaacag atgaatagag aaatggagag ataaagatga ttaataatgt gacattagta
      721 ggacgcttaa ccaaagatcc tgatttacgt tataccgcaa gtggtactgc agtagcgact
      781 tttaccctag ccgtcaaccg aaatttcacg aatcaaaacg gcaattgaga agccgatttt
      841 attaactgcg tgatttggcg caaaccagca gaaacaatgg cgacattagc taaaaaaggt
      901 agcttgattg gtgtagttgg gcggattcaa acgcggactt acgacaacca gcaaggacaa
      961 cgtgtatatg tgactgaagt tgtagcagat aactttcaac tattagaaag taaagcagcg
     1021 acagagagcc gtgcgcacgc tgatcaatcg tccaccagtc caagcaccgc cacctttgaa
     1081 caacggaata cagcgacgcc taacaacaat ggattgaacg cttcacagaa tccatttggt
     1141 ggacaatcaa tcgatattag cgatgatgat ctaccgtttt agtttgagaa agaaggagta
     1201 agcatggcaa aaaaattagt ctgacgatgg atctattgat acccgtaatt gcatttatag
     1261 acgggatttc tttgggcgtc actgaaaatc ctttggtaaa tagaattgcg aaaggatcac
     1321 tatttcttgt attcgtgtat cttgtctgtt cttatctttt aaaaagagca ggaaaggata
     1381 attccttttg gcgattgact aaagtcacag tagcagcgac agctgttata cttacggcta
     1441 ttctaccgct ttatatgtag agttaacggg aaatcgttga acttcttgcg aaatattgct
     1501 ataatgaaca tgaaaaagag gagttgcgct aacaacccct cattaacagc accgattaaa
     1561 gaacggtggc agaatattag ttatttggaa ataaccgctg acctgctaaa gtagacggtt
     1621 atttcttttt gtcattttta agcatattta caatcaaact aatcaaagca atggtaaaca
     1681 taccaaaacc aagaatggtt tgaattgtct catatgcgga caaaaggctt actcctttca
     1741 tagattttag cacttacatt cataagcacc accacctttc cagaagtcgc caccgtcttc
     1801 aaactttact gtcttgacta ttatagcata tctaaaatcg aataaaaatt ctcaatttta
     1861 gctgtttttg ctataatttt agcgaggtga tcgtgatgga acaatatatt ggtttactcg
     1921 ataaattagg gtgatcaaga cacagccatt gcttgtgcga tttacattac gcacgagtca
     1981 tgaaccaatc aattgtgtag tggccaatat agaaatagcg gataaattgc tgattttgga
     2041 agaaggcaag tatactattt cagtgacagg tcacttcaat aaacgaaatc aactagtcat
     2101 tgaatcaatg caaattagaa accctgatca ttttacgaga ttaatgggca tataaataaa
     2161 agaagagctg cagtcctaaa tgaggattgc agctcttttt gttttacata gaaacaagaa
     2221 aaatgaaaag gcctttttag gaaggcgcaa gcgccttctc aattgaaaaa cctctctctc
     2281 actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa cgttgcacgt tcactaagat
     2341 aaggggtctt gcaaagcaag acaaaataga aaaaacttcc tctcaccaaa aggttcgttc
     2401 ctgttcgcta tctctttatt gatttgccag accggcaaca agttgccttt tatcaccaaa
     2461 aagcgacaag gacaagggta aatggcacgc tgcacgccct tgtccttgtc gcttttagtt
     2521 gattcattct ggccaacaaa tcaataaaga aagaactaac gaaaggaagt aaaacctttc
     2581 ctaagaggag gcagaatcaa tcatgcgaaa gaaatttgat tattggggtg ttcccttcag
     2641 cgaattattc ccaaactatc atgcaccata tactgtcgaa tgcgattgtg gagaaagagc
     2701 gaaatgtatc aaaagctatt gtttgtatca atgtccaaca tgcgggaaaa aatatacctt
     2761 gtcttatggc gattatattt tgattgatga gaaaggaaag aaacgctgat gaaaacacaa
     2821 cacaaattat tgactgtaaa agaagtcgtt caaattttgg tggacaaaga agtcatttct
     2881 gaaaaagtag cagaagcttt acttattccg cggtactgga cgatagaaac ccctttagta
     2941 gaggatgcac cgactatgct atttgaagac ctagtgaaga ttgcaggata tgaactgcat
     3001 tatcaagaaa acatggaaac ttatgaaaac gaatattggt taaccaaatt agagaactaa
     3061 aaaggagaaa aaaatatggc tcaaaattac tatgacgaat ttgtcaaatt acctttggat
     3121 aaaatggcac aaaaaatgga ggatatgaca ttcctataca atgagacacg tgttccaaaa
     3181 aaacattata aggaaagatt gtcggtagct gtggaagaaa tgattgaatc gggtgtcgaa
     3241 atgaatctca ttgcgaccta ctatcgaacg cttgaagaac tgaaaaagca aaatggacaa
     3301 tggtttttcc aagccttact atgcttggaa gcaggcgtca agccaaacac tatcaaacct
     3361 tctgaatacc aagctttaga actaacgtat gggaaatttg tcgaaacgaa aaaagccaag
     3421 actgtttcaa gtgaatggtt ggactactta gaaaatgtca acaagtatgg cgcatgttac
     3481 atcatgaaaa aggaggacaa cgaaaatgaa taaggtgatt tatcaactaa caacggaaga
     3541 tgtgtcacac tttttaacaa acaaagaaca tttgaatgaa gtaatcagac ggattcaaaa
     3601 agactttagt tgtatggaag aaatgaagta ctttattcaa agagtcgaag aacaattgta
     3661 tttggaaagt gaaacgagtc aaacgaaggt tacagtcagc aataattatt tagacgtgta
     3721 taaacgtcgt cctgacacat taaaaaaagg agcggagctt ctacgactag aaacagaaaa
     3781 atatatcatg aatgtttacc tagaaaggag tgaactggtc gaaaactttg tcagctacac
     3841 gatttgcagg aaacactatt actcagaatc agaatattat tcaatttttg agagcatgga
     3901 attaatggaa gagctaaaca gcaaagatgc gacggtagac gattattacc ttgtatttac
     3961 taaatttttg acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcgc
     4021 tgtacccgaa gtatttttga gggaatatgt agaagaagta gataacccag gcgacgactg
     4081 gtacacgact ttaactgatt ttttagatac ctacacttac gacgatacag aagaaatcca
     4141 aaatgaactt gaccgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtcaata
     4201 accatttata ccgaaaagtg agaatgtgtg aaataaaaac aaaaaacctt gaaattaagg
     4261 gaatggttct tatttcgcac caatcgctcc aaccgccccc gccgcttagg tggtgcgaaa
     4321 taagcgagca aagcccgaaa acctaagaga aagaaggaga tttatatgaa caaaattatt
     4381 cccttattat tagtcctatt tgttggagaa atacttatgc gacttagtgc agctgtacat
     4441 ttaaacctcg cagtattttt ggtaatacta atgttttgtt tgatcttttg gcgatttgta
     4501 ttagccctcg taagtctact attcaagatg attgcagcca tatttttcat aggattattt
     4561 cgatgaaagg agagagtacc aatgccaatt gcaataggaa ataaacggtt acccgtgacg
     4621 ttggatgaaa agagacaaaa agaattgcag taaccaaagc agaagtacgg caaaagtgaa
     4681 tccaggatta tgtgtattgc gttagattta ttgattgcgc aagaaaaagc aggatttgac
     4741 ataccagcac tcaaaaagtg atcgcacttt gcgcacgtgt taatacgtgt tataattatt
     4801 ttgtaaggag gtatagtgca caatgccact tacgggaaaa gaaatgctta agttattgaa
     4861 aaaaatggct gggttgagcg aaggcaagag ggatttcatc atcatcttta taaggatggt
     4921 gttagaatca ctgttcctgt tcatgccaat caagatcttg gccgaggact tgagcgaaaa
     4981 attttaaagg atgcgggatt gaaataatct cgcattcgat taaagaaagg attgactgaa
     5041 atgttaagtg agcctttagc aaaaacctat cctgctattt ttagcccaga agaaggtggt
     5101 gggtacttta ttgaatttcc tgatgttcaa ggtgcttaca caggaattaa cgaagacgat
     5161 atatcttatg gaattgctat ggcacaagag gtgttaggta tggttttagc agattatatt
     5221 gagcatgaag atctacttcc agaaccaaca ccaatcaata agatttctgt tgaagatgat
     5281 tcttttacta ctttaattcg tgtagatgtt gccaaatatt tgaaagacac agaacttgtc
     5341 aaaaaaacct taacaattcc tcaatgggcg gataagctag gcaaaagggc tggcataaat
     5401 ttttcagtcc ttctaacaga atcaattgca gataaagcag ataacattct tcgttctgga
     5461 agaaataatt agaactcaaa agggtcagaa acttaaaaat ctttggcttc tttttttact
     5521 acaaagatgg cttctttaaa ttatttgcct gattgattta aaaaaattgc gcaaagacta
     5581 cgagctaatt aagttgctcg tggtcttttt ttatgaaaac tttttagat
//
LOCUS       DAXLPI010000138         5601 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXLPI010000138 DAXLPI010000000
VERSION     DAXLPI010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.142"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            25..696
                     /locus_tag="ACF3T5_002438"
     CDS             25..696
                     /locus_tag="ACF3T5_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFD3632966.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="ACF3T5_002439"
     CDS             755..1195
                     /locus_tag="ACF3T5_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632967.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="ACF3T5_002440"
     CDS             1207..1875
                     /locus_tag="ACF3T5_002440"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFD3632968.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="ACF3T5_002441"
     CDS             1939..2562
                     /locus_tag="ACF3T5_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFD3632969.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="ACF3T5_002442"
     CDS             2618..3004
                     /locus_tag="ACF3T5_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFD3632970.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="ACF3T5_002443"
     CDS             3217..4608
                     /locus_tag="ACF3T5_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFD3632971.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5219..5587
                     /locus_tag="ACF3T5_002444"
     CDS             5219..5587
                     /locus_tag="ACF3T5_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFD3632972.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
BASE COUNT         1862 a          922 c         1186 g         1631 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga tcgtttagga ggaaacagat
     5221 gtttacgaat gaaataaaaa tcatgcttta tgtggacgat gtagaaaaaa atgcaacgtt
     5281 ttggagagca gttggctttg cggaaaaaga tcgtcaagaa atggatggga cgcttataat
     5341 tgaagtagga gttgaggaca gtcaaacctc atttctgtta tatgatcgag catttgttga
     5401 gtcccattca cctggcacag ctactagtgc cccatctttg atgttttaca gtaaagatat
     5461 ctttggacta tataaaaaaa tgcagacgca atcagtcaca ttaggtgata tggtacagct
     5521 tggagaagaa tatgtcttta actttgctga tcccgatggc aactactttg cggttacagg
     5581 taaataatag aaataaagac t
//
LOCUS       DAXLPI010000139         5332 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXLPI010000139 DAXLPI010000000
VERSION     DAXLPI010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.143"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..278)
                     /locus_tag="ACF3T5_002445"
     CDS             complement(<1..278)
                     /locus_tag="ACF3T5_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632973.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGT"
     gene            complement(576..1550)
                     /gene="sgrA"
                     /locus_tag="ACF3T5_002446"
     CDS             complement(576..1550)
                     /gene="sgrA"
                     /locus_tag="ACF3T5_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFD3632974.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1746..2585)
                     /locus_tag="ACF3T5_002447"
     CDS             complement(1746..2585)
                     /locus_tag="ACF3T5_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFD3632975.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2770..3657
                     /locus_tag="ACF3T5_002448"
     CDS             2770..3657
                     /locus_tag="ACF3T5_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFD3632976.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4251..4946)
                     /locus_tag="ACF3T5_002449"
     CDS             complement(4251..4946)
                     /locus_tag="ACF3T5_002449"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFD3632977.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4930..5328)
                     /locus_tag="ACF3T5_002450"
     CDS             complement(4930..5328)
                     /locus_tag="ACF3T5_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFD3632978.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1699 a         1076 c          782 g         1775 t
ORIGIN      
        1 gttccctcta aaagtataga ataaacttta ctcagtaaaa actgagaaga aaactcgact
       61 tttatcatct tgttttctga aagaaaagat aatttatatt ctggatacct ttcgcaaaac
      121 gattctgctt gttgaatcac cgaattcatc ttagccaggg tgtagtttag atctaaagcg
      181 atatccttca ccacatataa tttctcgtct ctttcataac aatagttcaa aaattgatat
      241 acttgccttt cattgatatc aataaattct tcaaacaaag tacatactcc tttttatcta
      301 ttatttagac ttattccgac agctacatga ataacgtaca aatagattcg ttactgattt
      361 ttcagagata ctaccatgcg cagtgtgcat tgtcaattat tcttgctctt attttccatt
      421 tgtgttcctg ttatttcctg tttctcaaat atgttagtaa acaaaaatag atctggcaaa
      481 gctacaactt cgccaggcaa tacagcactc tcgaatgaaa tattcactca aaaatctatc
      541 gttttattca attttagatc tgttgctttt attctttatt tcactcgatg gaagagagaa
      601 cgaaaatagc ttgctcctac aatcaaaatg agtaacacac ccatccatgt aattagcttg
      661 ttcaaggttc tactaccagt ttctggtaaa tcgctttttc tattatctgt ttctttcgct
      721 ttttcttgat gatcaattcc acgagaaaca aaatcaggct gttttagcac actttttagg
      781 ttgctccctg catttctctt ttgttcctta gttggtatga taggtaccaa atcaagttca
      841 ttatttactt ttcctgtgct gctttcgctg ctcgtgcttc ctgtgctact ctcactactc
      901 atgcttcctg tgctactttc actactcgta ctctccgtac tgctttcgct gctcgtgctt
      961 cctgtgctac tttcactact cgtactctcc gtactgcttt cgctgctcgt gttttctgtg
     1021 ctactttcac tgctcgtact ctctgtactg ctttcaccgc tcgtgttttc tgtactgctt
     1081 tcactacttg tgctatccgt actgcttggc ggtccaacaa ctgtccaact gcttggtttc
     1141 aaattctccg tgatcgtcat ctgtccatca taaaaaacaa tcaaatcaac tttatcttta
     1201 ctcttgatct catcaatcga tagttctgca gaaatcatta ttgtatactc aataccagcg
     1261 gtccattgta cgtcttttaa ataaacttcc ccaataactt tttctgaact tccattacca
     1321 tcccaaccat atgttagaac agcatgtaca tcactagcat tcacgctttt gtttggtttg
     1381 actggaatag taatgacttg ttgtgtcatg tcataaacaa tccgatcagt tttcacagta
     1441 aatgaaagat tctcatttgc ccgttcattt gcagaagtac tgattccaat cattgctgca
     1501 attatactca gcccaagaat aacaaataat acggtcgctg ttttcttcat ttctatcact
     1561 ctccatataa ttaccttcta tttgataaag gaattatata aaatgaaaat gatgagaaca
     1621 cgacagtcag ttgtgaaaat ctaacaaaag atgtcgtgta ttcgcttgaa tttgtttact
     1681 ttttcgttta atcaatgaca gacttaaaaa agcaagaccg atatcagcct tgctttcttc
     1741 atcttttatt gataaacagc agtgtatttc ttcatccgct ttgttttata tgcatagagt
     1801 aacaaaccaa caatcaagac taacaagaat aatgtagttg atatagatgc ttcagcccca
     1861 aagtttcccc cagtaattat tgtattttgc gattggatca gagtggagaa aaaaccttgt
     1921 tcttgcgttc cgctgactgg tatacccaaa atagctcctt gtgtccaatt ccaagtaata
     1981 tgagcaccaa tagccgtaaa taaacttcct gtactgtaga taagtgcgct gaaaaagcaa
     2041 cctgctaaaa agatattgaa aactgccaga aatgtcatgt taggattcaa tccgtgcaaa
     2101 agagcaaata atgcagcaga tccaatcatt gctattaacg gaccaaatct aagcatctga
     2161 tgctgaaaaa agccacggaa taaaagctct tctgatgccc ccactgcaat aaataaaata
     2221 aaactcgtta tcagcggcac aatgtttgcg tttcctgaat ctaaccgata cgcccccgtc
     2281 aaatataatg gactagtaat aagcagaaac actccaattc caacaacgag acctcccgct
     2341 aaataccccc agtcttttcc tcgaacagaa acaccataat cttctattct ttttccttcg
     2401 accttactag caagaaataa agctagtaaa aaaacagcta aatgtccgac cgtaccggca
     2461 attgctccaa tccattcact atttccaata aacatatccg caagtgcatt attgacaaga
     2521 attcctaaaa caaaaccaac taagactatt aaaacagatc caatcaaccg aataatcctg
     2581 atcatctttc atccctcctt tatattaagt ttaacaaccc ttttctttct tgtctcgtga
     2641 aaactatgta aatgttctcc atcatagaga gaatagattt ttacgaacta agaatatcta
     2701 cagcaacctt acgaaaatga aagactgtct ccctaattct tctgttagac tgtttttaat
     2761 aaggaaagga tgggaaattt tttgaaaaaa caactactaa ttatgtgcct ttcagcatgt
     2821 gcaattttac tattctcagg ttgtagcaag tccaatgaaa cgattgcaac gtttaaaggt
     2881 ggaacactta cagttcagga tttttatgat aacccaaaag ttaaatcaaa taatcaaaca
     2941 ctattaaaaa aaatgattgt cttcaaagca tttgatgatg tatacggaaa agagatttct
     3001 tcaaaggaag taacaaaaga atataatgaa caaatcaaaa aactcggtcc aaactacaag
     3061 gaacaattaa aagcagttgg ccaaacagaa gagacatata agaaactctt taaacagatg
     3121 ctggctttcc aatacggttt gaaagccaat gtaaaattaa cagataaaga tctagacact
     3181 gcttggaaag aattctatcc agaagttagt acacagatca ttctattttc gactaaagaa
     3241 gaagcagata aagcaaaaaa agaagcaaat gaaggagaaa atttctccaa gcttgttcaa
     3301 gcttatggaa aaaataaaac attaaaggaa actgatggaa aaatgaattt tgattcaacc
     3361 aatccagaga ttcctactga agtaaaaaaa gcagcattta aactaaaaaa cggggaagtt
     3421 tctgatatca ttcctgtaac agatccaacg acttatcagc aaagctatta tctagtaaaa
     3481 atggtaaaaa aacaggataa aggttctaat aaagacaaat acaaatctga gctagaaaaa
     3541 attgctaccg aagcaaaaac tcttgatacg gaattcatgg ataaaacaat ccgtaaagtg
     3601 atgaaaaaag acaatgtaac tattaaagat ccttatgtaa aaaatatatt caaataaaca
     3661 aatttgaccc aattggggat gtggcaaaat ttgagaagtg atttttggaa attccttctt
     3721 atttttgagg caaaccactt ttgccacaac ttcatcgggg ccttttttta tttgtaccaa
     3781 aacaaatcac tggatttgtt acaatcatga ggatataatt taaaaaagtt ttttatttct
     3841 atcacatgaa tcaatgcgcc attaagtaga ttctttcatg cttccctatt ttttcacttg
     3901 ccgtatcgct tcaaaaacaa ctatcataaa ctcaaactcg actgaaaagt tattttaaaa
     3961 aagactaaaa atatcccttc tggacttgtt cttcttattc tgttatacta gagaaacacg
     4021 aaagatattt ttatgcgaca cttttcattt gtttttatga aatttattca aaactggcgt
     4081 aacttcagag ttacaaggat gtattagagg ttagggaata catcgtttaa atgagaaata
     4141 agtaatagtt agtaatagtc acccaaactt taaattaatg aatattgaat cgtcagatag
     4201 cctcctcgag ttttcgagaa ggctatcgtt tattttatgg agaaattgat tcagaagtaa
     4261 tcagcgacct tttttagttc accaatcgtt tctgcttgga aagtttgcac ccaatcttct
     4321 tggaagcccc tcactgcttt atcaatcgag ggcatctcta atacttgatc tagtagctct
     4381 ttacctaacg ttatcgcatg agctccagat cgaatggctg tagtgacttg ttcacaattt
     4441 ttgaaactag ctgctaatat ttttgttggt ctttgcgtac gttcgatctc agtccgcaac
     4501 gaatcaatca ctttcatagc atcaatgcct tgattttgca tcctgttata ataaggtgca
     4561 atataatctg ctccattatt aatagctaaa tacccttgta attctgtgta aatagctgta
     4621 ccggtaatca aataaccttc ttttttcaac tttttgatcg cttgaaggcc tgcttgattc
     4681 accggcacct tgataaacgt atcttttccc agttcttcta tgataactcg agcatcctct
     4741 atcatttctt cttccgttct tcctattgtc tgaatatgga taggaatctg tccgattatt
     4801 tttttgattt ctttcatatg cgagaagaag tttgacgctc cttctttttt cagaattgta
     4861 ggatttgttg ttacgcctgt taaattcaaa aactgttgac aagaataaat actttttgta
     4921 tttgctgtat caagcatgat ttccatatag ctttccccca ttctgatcct cttgatcgat
     4981 tttaatgatt gtcccctttc ttaaatgcgg catttcctta tcttctacat acattgttcc
     5041 tggaagttcc ggctcatgag atccatcaaa ttttatcgtg atatgtccta attcattcaa
     5101 attagtttgg acaacatctc ctactgctgt gattacatac atttgtttat caaaacagag
     5161 cttcatatca caagttattt cttttcttac ctgattcaat tcaatcttat aacaataatc
     5221 tgttaatgtt tccggtgctt catctccaaa taaaatcatt attttttctt tcctgaatac
     5281 ttctgcttct ataccaacat ctgttacaac cgtttcaaag atcgtcatct ct
//
LOCUS       DAXLPI010000140         5270 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXLPI010000140 DAXLPI010000000
VERSION     DAXLPI010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.144"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            205..1479
                     /locus_tag="ACF3T5_002451"
     CDS             205..1479
                     /locus_tag="ACF3T5_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3632979.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            1533..2960
                     /locus_tag="ACF3T5_002452"
     CDS             1533..2960
                     /locus_tag="ACF3T5_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFD3632980.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            2973..3845
                     /locus_tag="ACF3T5_002453"
     CDS             2973..3845
                     /locus_tag="ACF3T5_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFD3632981.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            3940..4935
                     /locus_tag="ACF3T5_002454"
     CDS             3940..4935
                     /locus_tag="ACF3T5_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFD3632982.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
BASE COUNT         1821 a          733 c         1023 g         1693 t
ORIGIN      
        1 ataaaaaata gtgccattaa aaaagaaaag cgacacacca ataaaagtca tatcctgtga
       61 cattatatgg cattatccaa atagaataac cggttgacat gtaagcggat ttcttttata
      121 attttaggag tacaagtcct tttctaaaaa tgttaaccgg ttatcatgaa aggggataca
      181 aatatatcgt tggaggataa aaatatgtca aaagttaacg tatttaaaaa tcaatcttat
      241 ttacaaagtt cagctacatt attactattt tttgcttctt ggggtgtttg gtggtcattt
      301 tttcaacttt ggctaacatc tgaatcaaat ggtttagggt tatctggcag tgctgtagga
      361 acagtattct cggcaaattc gttagttacc ttaattttga tgtttattta tggaacatta
      421 caagataaat tgtatattaa acgaaattta ttaatttttg cttctgtatt agcgacactt
      481 gttggaccat tttttatatg gatatatggg ccattgctag ataacaattt taatttaggc
      541 attattatgg gagcgctatt tttgtcagct ggatatttag cttctgtagg agtttttgaa
      601 gctgtgtcag aaaggtttag tcgtttattt ggctttgaat atggacaagc aagggcgtgg
      661 ggatcatttg gttatgcctt ggtagcgctt ttggcaggat ttttatttgt aaaaaatcct
      721 catttaaact tttgggcggg atctttcttt ggttctttac tattgttaaa tttattattt
      781 tggaacccta aagttgaacg ggaagcaaat caaaatttta atcaagaaca agctgaatca
      841 aatagtattc cttctttaaa agaaatgttt gatctaatga aactgcctca attatggacg
      901 ataatcatct ttattgtttt tacatggaca ttttatacgg tattcgatca acaaatgttt
      961 ccgggatttt atactggttt gttttcaaca tcagctaatg gtgaaaaaat atatgggaca
     1021 ttgaatgcta ttcaagtatt ttgtgaagcg ttaatgatgg gaattgttcc aatcattatg
     1081 agaaaattag gggttcgaaa tactttgtta ttaggtgtaa ccattatgtg tgtacgaatt
     1141 ggattgtgcg ggtttgcctc gacaccatta tctgtttcat gcataaaaat gttgcatgct
     1201 ttagaagtac cattatttac attaccaatg tttcgctatt ttacacttca ttttgataca
     1261 aagctatcag caaccctcta tatgatagga tttcagatag ctgctcaaat tgggcaagtg
     1321 attttatcaa caccattggg aatattaaga gacaacgttg gctatcaacc aacatttaaa
     1381 attatttctc ttattgtatt actagcaggc atatatgcat tctttattct taaacaagat
     1441 gatagagatg ttcaagggga tccatttatt cgaggataat ggaaaccaac agaataaaaa
     1501 acaattttat ctagaaagtg agcgaatata ttatgacaaa aagagctata gaattaacaa
     1561 atacacgtta tcgtttggga tatcatgtgt ctgcaccatc aggatggatt aatgatccaa
     1621 atggattttg ctattttgat gggtattatc atgtgtttta tcaacatcat ccatatagtg
     1681 ctgaatgggg accaatgcac tgggcacatg ctagaagtaa ggatttagtt cattgggaat
     1741 ctttaccact tgctttgact cctggagatc aagaagatga aggtggttgt ttttcaggaa
     1801 gcgctattga aaaaaatggt gtgttgtatc tgttctatac aggacatcac tattatggag
     1861 acaatgatcc agatcatttt tggcaaaatc aaaacatggc gtatagtacg gatggcattc
     1921 actttactaa atatgaaaaa aatcctgtaa tagcaaaagc accagaagat aacacacatc
     1981 atttcagaga tccaaaagta tggaaacacg atgaaaaata ttacatggtt ttgggtagtc
     2041 aagaaaaaga tggattaggt cgtgctattg tttatcagtc gttagattta ttaaattggg
     2101 attacattgg accaatttct aaagcgaagg aagtacttac agaaggattt atgtgggaat
     2161 gcccagactt ttttgaatta aatggaaaat ctattctttt attatcccct cagggaattg
     2221 aagcaaatgg aaaagattat ttaaatctat atgaaacagg ctactttatc ggtgattatg
     2281 attatgagac agcaaaattc attagaggaa atttttatga attagataag ggacacgatt
     2341 tctatgcaac acaaacaact ttatctccag atggtagacg tattgttatg gcctggatgg
     2401 atatgtggga aagccctatg cctgaaaaag tagacggatg ggcaggagca ttaacgcttc
     2461 ctcgagaatt agagttaatt ggagatcgtt tgtatatgaa acctgtgaga gaattagaac
     2521 aacttcgagt tggcagcggc gttgaaacga cagttaattt ggcaggagaa ataataatcg
     2581 ctcaagatgc ttcattcaaa gaagctctaa ttgatgtact attgactaaa tcaaaaaatc
     2641 aagaagtcaa attttcatta aaaacaaaag atgaagagtt aatcaatatt ttttattcta
     2701 aggataaaaa tgaatgtacc ttaactcgta acggcaaaaa tgatccaagg tatggaacca
     2761 ttcagatgtg tgatagatta tcacttcgag tgtttatcga taaaagttcg gcagagattt
     2821 ttattaatga tggcgagctt gtttttacag agcgttatta tacagaaaat caaccaagta
     2881 tgcatttgag tgttagtgaa gaagcttttg tctcgagtac aatttatcat ctaaaaaaag
     2941 atgcaatttc ttacgagtaa ggaggtttta tgatgttaat tggtagcata gaagcaggtg
     3001 ggactaaatt tgtatgtgcc gtgggagacg aggattaccg agtacaagac caaataattt
     3061 gtccaacgac cacccccagg gaaacattga aaaaaactgt tgactatttt aaacagtttg
     3121 atataaatgc attaggtatt gcatcatttg gtccaataga aattcgaaaa aattcaccaa
     3181 aatcaggata tattacgtct acaccaaagg tgggatggcg aaatgttaat gtgttaggtt
     3241 atttacaaga tgagctgaat gttccaatgt cttggacaac ggatgtgaac ggatcagctt
     3301 atggagagta catcatgtcc actttatcga atgaaaaaat aaattcatta gtttattaca
     3361 ccgttggtac tggcgttggt ggtggagctg ttgttgacgg aaaactatta ggatcgtttg
     3421 gtcattcaga aatgggccat acgtttgtaa aacgccatcc tgatgatatt gattttaaag
     3481 gaatttgtcc ttatcatggt gattgtttag aaggcttagt tgcgggacca acgtttgaag
     3541 cacgttttgg aaaaaagggt gaagacgttt ctttaacgga tcctatttgg gatgtaatgg
     3601 cctattatct agcacaagct gtgattcaaa caacattgat tatacgaccg gataaaatag
     3661 tatttggtgg aagcgtagta aatgaaaaat tgttagaaaa aattcgtgtt aatgttaata
     3721 aattattgga tgattacgtg gaaataccac cgttaagtaa atatattacg cgtcctttaa
     3781 ttaaaaataa tggatcggca acactaggaa actttgcttt gggcttgaat caattacaag
     3841 aataaaacat agaaaaaata tcgattatca ggtatagtag ttctatagag ttcttttttt
     3901 cgatagttaa ttattgcatt atttaggaga gacaagagaa tgcgcccaaa attagaagat
     3961 gttgccaaaa gagcaaatgt atcaaaaaca actgtttcac gtgtattaaa caatcgtggc
     4021 tatttaagtc aaaaaacgat tgataatgtt tataaggcta tagaagaact caattatcaa
     4081 ccgaacgttg tagcgcgtca attatttcaa aagaaaacta acattgtcgg gttactattt
     4141 cctacagtag ccaatccttt ttttagtgaa ctcgttgagg cattggaaaa aaagttatat
     4201 gaaattggtt ataaggtatt aataggcaat tcgatgaata ataaagaaaa ggaaacaaat
     4261 tatttaaatc agttattgtc tgatcaagta gatggtttaa ttgttggtac acataaccaa
     4321 ggaattcagg aatataaata tcagaattta ccgattgttg caattgatcg agtgatgaat
     4381 gaagatattc cagttgttga atccgataac tacaatggtg gaaaacttgc aaccaagtta
     4441 ttaattgcac aaggtgcaaa aaatattatt catacaaacg gtccaattga cttacagact
     4501 ccggctaatc gtcggagact agcctatgaa gatactatga aagcatatca gttaattcca
     4561 cgaactgtga cattagattt caatatttct tatgtaaaga aaaaacagat ttttttccaa
     4621 atgtttgagg attatcctaa aatagatggt atttttgctt caaatgacat cgatgcggcc
     4681 ctaatattac aagtagcaaa agaaaagggg ttaaatgtac cagctgattt attagtagtc
     4741 ggatatgatg gtactttaat gactcggagt atattaccag atctgacaac agtaatacaa
     4801 ccaattaatg atatagcaga cacagctgtt gctattttga tgaaacgaat aaataaagaa
     4861 gaaacaaaaa aggaatatat tttacctgtc acattatgga tggggaagac atcagtaaag
     4921 aaaaagaaca attaaattta taaagactat caatgataat ttcattatta attcgtgata
     4981 taaaaaattg ttaaatctct tataatagac tatgatacaa aatcatgctt ttaatctgtg
     5041 tagatgttgc caaatatttg aaagacatag aacttgtcag aaaaacctta acaattcctc
     5101 aatgggcgga taagctaggc aaaagggctg gcataaattt ttcagtcctt ctaacagaat
     5161 caattgcaga taaagcagat aacattcttc gttctggaag aaataattag aactcaaaag
     5221 ggtcagaaac ttaaaagttt ttgatccctt ttttattcct catttcatag
//
LOCUS       DAXLPI010000141         5092 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXLPI010000141 DAXLPI010000000
VERSION     DAXLPI010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.145"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..3044)
                     /locus_tag="ACF3T5_002455"
     CDS             complement(<1..3044)
                     /locus_tag="ACF3T5_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFD3632983.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDIIENPGENIPAGYHKVT
                     FTAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEF
                     VSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVS
                     AAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
     gene            complement(3661..4095)
                     /locus_tag="ACF3T5_002456"
     CDS             complement(3661..4095)
                     /locus_tag="ACF3T5_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632984.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(4112..>5092)
                     /locus_tag="ACF3T5_002457"
     CDS             complement(4112..>5092)
                     /locus_tag="ACF3T5_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFD3632985.1"
                     /translation="KTRYYLFGPFRANNIDKVFFKLKMNNLNVAMADRERLYNSLQNL
                     TLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPE
                     IYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQ
                     TSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPL
                     ISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKI
                     MLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1492 a          982 c          719 g         1899 t
ORIGIN      
        1 ccttctactg ttggctcata tttatcggcg tcagaacgat tgtctgtcac tgtaacatct
       61 acttcaactt catcagatga tccatctgga tatgtcacta caacagtccc ttttttattt
      121 ccagctgcgc taacatctac tttttctttc caagtatact tagtatcttt tggtaaatct
      181 tctttgttct taattccctc aaatgcgtct ggttccttat tcaattctgt agtcactttt
      241 tgaccttcag gattatattt gtcagcgtca gatttatcat ccagttttgt tgcacttgat
      301 acaaattctg tatcatctgc tgtaattggt tgagttgctt cctctggcca tttcgcatct
      361 gtataaccat cttttgcttt caacactgga agtttatctt ctggtaagct tacgccgtct
      421 ttcactgcaa agactgttgt tccactttca atacttgttc cttctcctgc ggtgaaggtt
      481 actttgtggt agcctgcagg aatgttttct cctggatttt caatgatatc atccagtttt
      541 gttgcacttg atacaaattc tgtatcatct gctgtaattg gttgagttgc ttcttctggc
      601 catttcgcat ctgtataacc atcttttgct ttcaacactg gaagtttatc ttctggcaag
      661 cttacgccgt ctttcactgc aaagactgtt gttccacttt caatacttgt tccttcccct
      721 gcggtgaagg ttactttgtg gtagcctgca ggaatgtttt ctcctggatt ttcataaata
      781 tctttactta cagttactgc taaatcggtc gtgcctacag aaccatcttg gtaaacaatt
      841 ttaataggaa ttgttccagt aatcggctcc gattctccta cacctttagc tggagtgaat
      901 gtaacaatac cagtgtttgc atcaacactt gcattaccga agtacttatt atagacatct
      961 gaaattgaaa aggcatcttt agctaaagga acatctggtt tatcaaatgt aggttttcca
     1021 ctttgaactg tcgtttctgg agttgttgtt ataggtacgt atgttgcatc attttccata
     1081 gcttgagaag cttttacagc aaactctaca tccacgtcga aagttcgatt tccgatctta
     1141 acagttacaa tacctttcgt actatcagta acgttagaag tatcaggctc tgttttccat
     1201 gaatactctg catcttcagg taagttttta ttattccgaa ttgattcttt agcatctggt
     1261 ttaaatccaa ctaccacggt ttgtttatca accgcttttg gtgattcctt aataacggtt
     1321 gaaccttctt ctggtttatc aaaacctgga gaaacgattt ggatttttaa ttcatctttc
     1381 gcgattaatt tacttgaatc tacacccgta aattcaaatt ctacagtatc tgaaattgga
     1441 gccccatctt tttcatttgg agcgatagtt ttttctgcta atacagtacc ttctttatca
     1501 acaagttggg ctttgtgacc tgttcccgct aactcgtgga tgaatacttt ccccttaact
     1561 gttgtgtcaa catcatatac ttcttctaat tcaggtttcc cgatatatgg attcgtgtct
     1621 ccgctctctt ctttatcgtc agggtttcca tcaccatcaa tatcttgcaa ataataataa
     1681 cctgttccat aagtgttctg gattaatttt ttattcgtat ccgacaacca agaatcaaag
     1741 atgaaatctt caccaaatgg tttattttct tcaacttgtg ctgttgcttt catatcttct
     1801 aagatttcat ctaatggctt agtcaattgt aaaacatatc gaataacgac tggacgtgga
     1861 cttgcattag caaaatcgat aagtttagtt aaatcattat tagttattga acctatgcca
     1921 taattttcat gaccttcccg tcgctcgatt gctaaatcag ctacttttgt tccggattca
     1981 aacgaaacat catatgttac tttgtcagcc tgaaccatgt gcatctgtat acccgctaca
     2041 tatggaacta atcgttcatc aattttgtaa ttagcatacc aaggcgtgcc ctttgcggaa
     2101 gatgaatatg agaaattata atttttcgtt aacttatgat ctaagattat agctccatta
     2161 gcacctgtgg atcctgaacc atcttttgtt tgagtattag gtttctcata ttttgcactt
     2221 actgatccat gtttaaactg gtcaggatta ttttcagata catctaagct attatagaaa
     2281 tcatctgaat ctgttaaaaa gtagcccgta ctatcaatac ccggtacaat tttatcatcg
     2341 tcttgatgac ttaccaagta gattcgatac attaaacgat cggtcttgag attgtctatg
     2401 cctatctctt taaaaatttc atctaggctc ttttcaaacg taattacccc attaggagcg
     2461 gtttgcgtat cggttgtttc tgccccagca aataatccat cacttgcacg aatataattg
     2521 accttccaaa tatttgtagg tctacccagc gaatcattaa ttcttacaaa ggtccgtcta
     2581 gaagatccaa ttcctttctt tggatcgacc tgaatttttt ctacatattt agctaatcgt
     2641 tcatctaatt gaagacggat ttcccagttt ttagttgatg ctgcaatatt atgttttccg
     2701 taaacttcaa atccaattgt attgcctttt agtgtgtctc cagttagttc ttgaggacta
     2761 aattgtaaat ccgaaaagtt ataatttttc gtataaccag gattctcctg aaaagtattc
     2821 cccgaagcta ctttgtcacc tacttgtacg gagcttatcg ctgtttcaag tgggctgctt
     2881 ggagaaattt catcttgtgc tttaactagt tcagttgttc ccaagacaag accaacacca
     2941 atcaaaacag atgcaacccc aacacttaat tttttgatac tgtacttcaa tactcgtttc
     3001 tttgtttttt ctagaaatac tctcttatta ttcttgctaa ccattattca aatcaactcc
     3061 cttaataatt actttcctag taagaataaa agataaatct gatacgtatt attcacagaa
     3121 taggaatctt actaacctta tgttaactaa tttacttaga gaatttcatg aacattacaa
     3181 caaaaaaagt acatttcttc atgttttttt cggatatttg tgctttttaa ccattcagca
     3241 gctgaaaaca tataaaaaga tatgtatgta ttctttctga aattaagttc atttctatgc
     3301 gatgaagcat gttttcaaaa tattaccctt tcttagatta aacttcagag tattcaaata
     3361 acggaataag gagccttatc ccgcattact gaatgagact gtgacataag taaaaaggtg
     3421 acttcaaatt ccttcttcgg aatttgaaat caccttcttt ataattataa agtatcggtt
     3481 tcacagcatt tctgaatgat tcctagaatc ttaaacgaat gattggagtt atcaacattt
     3541 tttcactcgt ttttagcgaa acaaaaaatg acatttgtca cagcctcatt aaattattga
     3601 ttctttttct ttataattga attgtccata tttaaagtgt ctagtactct acatgaataa
     3661 ttaattgctt tcactcaaat acttataatc tattaacttg tagttgttta acttttctga
     3721 aaaagttaga cccacgtaat aatcttccca gctttctctt tcaattaact gatccaattt
     3781 ttgataatcc ataaattcat catgtatcaa aaagaataaa taacttgata ttttagcatc
     3841 tagcttaatc agattaaata atgtttgttt aaaattgcta ccattaatat gactgataga
     3901 ttctctagct tcttcacata aactttttac tttattaatt actactttgt catattcatt
     3961 taacacacta ttattaccat tacttagacg ctcagattct acgaaacaac tcaaatcaga
     4021 aatttcccaa aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa
     4081 atgatatagt ctcataataa aataaaacct ctcagctaat gttgttgaaa ttgtccacta
     4141 aacctaattg atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt
     4201 gtgaagaatc tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca
     4261 acattatttt agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca
     4321 tttcattctt aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt
     4381 cctttacagt aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta
     4441 agggagaaag atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag
     4501 aatccctcac ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag
     4561 catctctact ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta
     4621 acttttcaaa aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac
     4681 ttggaattat tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat
     4741 ttttaacata ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat
     4801 tttgtgataa cagattatct attttgattt gctcgatagt tttacttaga ttttctgtat
     4861 attcaattaa cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta
     4921 aaatcagaat atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat
     4981 agagtcgttc tctatcagcc attgcaacat tcaaattgtt catttttagt ttaaaaaaaa
     5041 ctttatcaat attatttgct ctaaatggac caaataagta atatctggtt tt
//
LOCUS       DAXLPI010000142         5042 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXLPI010000142 DAXLPI010000000
VERSION     DAXLPI010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.146"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..182)
                     /locus_tag="ACF3T5_002458"
     CDS             complement(<1..182)
                     /locus_tag="ACF3T5_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFD3632986.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACF3T5_002459"
     CDS             517..951
                     /locus_tag="ACF3T5_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFD3632987.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACF3T5_002460"
     CDS             974..1294
                     /locus_tag="ACF3T5_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632988.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACF3T5_002461"
     CDS             1435..1626
                     /locus_tag="ACF3T5_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632989.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACF3T5_002462"
     CDS             complement(1697..1954)
                     /locus_tag="ACF3T5_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFD3632990.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACF3T5_002463"
     CDS             2112..2375
                     /locus_tag="ACF3T5_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632991.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACF3T5_002464"
     CDS             2399..2587
                     /locus_tag="ACF3T5_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632992.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACF3T5_002465"
     CDS             complement(2670..2855)
                     /locus_tag="ACF3T5_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632993.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACF3T5_002466"
     CDS             3070..3441
                     /locus_tag="ACF3T5_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFD3632994.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACF3T5_002467"
     tRNA            complement(3591..3663)
                     /locus_tag="ACF3T5_002467"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACF3T5_002468"
     CDS             complement(3709..4137)
                     /locus_tag="ACF3T5_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3632995.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACF3T5_002469"
     CDS             complement(4197..4955)
                     /locus_tag="ACF3T5_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFD3632996.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT         1757 a          784 c          986 g         1515 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 ga
//
LOCUS       DAXLPI010000143         5016 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXLPI010000143 DAXLPI010000000
VERSION     DAXLPI010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.147"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(107..1873)
                     /locus_tag="ACF3T5_002470"
     CDS             complement(107..1873)
                     /locus_tag="ACF3T5_002470"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632997.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1833..3587)
                     /locus_tag="ACF3T5_002471"
     CDS             complement(1833..3587)
                     /locus_tag="ACF3T5_002471"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3632998.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3697..4266)
                     /locus_tag="ACF3T5_002472"
     CDS             complement(3697..4266)
                     /locus_tag="ACF3T5_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFD3632999.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4284..>5016)
                     /locus_tag="ACF3T5_002473"
     CDS             complement(4284..>5016)
                     /locus_tag="ACF3T5_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFD3633000.1"
                     /translation="TENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDI
                     NFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQ
                     FFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLS
                     KTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNG
                     GIKKDIKLRVEDSTLIEQIFNDITK"
BASE COUNT         1666 a          944 c          678 g         1728 t
ORIGIN      
        1 ttctttcgtg tattcaaaat tagatgctgt ttaataaaat tattcttttt gaaaaagaat
       61 tataactata tcccaaaaat tgtaggttaa tttaaagcct atttcatcat cgtgattgag
      121 cttctttttc atagacgaat ttctcagata gtttagtttt ctttatgaga tttttgtata
      181 tttctgacct tcgatacaac tcattatgag taccttgatc aactagtttt ccttgatcca
      241 tcactacaat tttatttacc ttttcaatag acttcaagcg atgcgatata attagtactg
      301 tcttatcttg tattaaacga tttaaacttt cctgaatacg tttttcattt tccgcatcta
      361 aactggcagt aatttcatct aaaattaaaa ttggcgcatt tttcaaaaaa gcccgtgcta
      421 tggataggcg ttgtcgctct cctccagaaa gtgaacttcc gttttctccg accaatgtat
      481 gataaccgtc aggtagtttt tcaataaagt cttcgcagtt agctaaacga gctgcttttt
      541 tcacttcctc atctgacgct tgactatttc caatacgtat attttccaaa atacttgtat
      601 taaacaaagt tacctcttga aaaacaatag cgattttgct aaacaaagaa gctggagata
      661 cacgtttaat atcataccca tcaattaaaa tacagccttc atcataatca tacagtctag
      721 acactaattt caagatacta gttttcccgg aaccactagc acctactaac gcagtcactt
      781 ctccttgttt agccgtaaaa gagatatgat ctaaaattgg ggtattgtta tcatatgaga
      841 aagagacatc tcgcaactca atgtcaaaag atttcagagg actatctgat ccctcttgga
      901 tactggtttc cttcatagct ctaattcgct gtattttagg agctaaataa aatatttcaa
      961 gcactgcttc tttcatagaa tcgaaggaat cctttatttt tattgcggct agtaagtaac
     1021 caacgacgta gagtatagtc acttcacccg ttaacagtaa gtgaacacct actagaatga
     1081 ctactgctaa actaacatat gagaaaatag aggataaggc catgactgaa aaagtactta
     1141 attctacctt taaatgaatc ctttcactct cttccatttt tttgaaaagt ctgtcttgaa
     1201 cttttttaga tagattaaag ctattaatct cttgctgcaa ttctatagtt tcttgaaatt
     1261 cttccgagtt ttctctcaaa gtatcgtaat atttagtatt ggctttcgtt tgggatttct
     1321 ttgataacaa gattaagaca aaactaaata acgttggcaa aataacagct aatcccattt
     1381 tgacattacc aaccaaaagc atcactgaaa taaaagggaa aaacagagcc ataccgccgg
     1441 ctttaggtat tgcatgactc atcgcatgct caataccttc aacgtcagac atgatagttt
     1501 gagataaatc tgataaatta tgttttgaaa aataggataa tggtaaattt gacaaatcgt
     1561 ctgcaatttg cactcttaaa tgcgcacttt ctttataagt actattatat aagttttcat
     1621 actccctatc taacaaccaa tacatgaaaa gcaaagtaac aaacgatacg gcaaaataaa
     1681 gccaacgggg ctttgtattt tctaaaacta attcgtctat cagcatcatt ataatgaatg
     1741 ctggtaccat attaatgcag tatacaaaaa aactagaaat agaagcttta gttaaagctt
     1801 ctgcgccctg atcacttaaa gcaaatttgt ttttataaaa acttttcatt gtcaagcctc
     1861 caatcatttg cattctcata attagtgacc aattgtttat acacttgatg tttagccatt
     1921 aattcattat gatttccacg ttctactatt ttcccttctg acaagacaag aatttcatct
     1981 acagatttaa tgctggataa tcgatgagct atcataataa ctgttttttc ttttatcaga
     2041 tgttgaaaag ccttttgtag ttcatactca ttatctggat caatagatgc actggcttca
     2101 tccataataa caatctttgc atcttttaaa attgctctgg caatagctat acgttgtttt
     2161 tctcctcctg acagataaac tcctttacta ccaattaagg tatgctctct ttcgggaaat
     2221 ttttctaaaa tcgattcgca accggctaat cttagcgcgt caaatacttt acttgctggc
     2281 gcttgaggat ttgctataga aacgttatcg taaatactct gattgaatag tttcgtatct
     2341 tgaaagacaa aagcaattgt gttaataatt gtgtcttttg aatagtgatt taaaggctta
     2401 ccaccaattt taatctgtcc cttatccaca ttgtagaaac ctgaaattag tttagctatc
     2461 gtactctttc cgcttcctga ttttcctact aatgcataaa ttcttttttg ttctaaaact
     2521 aagttgaaat tttctaaaac acactcttct ttgtatgaaa agctgacatt ttcaaatgca
     2581 atgtcacaat ttttaatctg tgtctcgttc ccatatttta gttggtcttg cttcatttct
     2641 aaataaaggg agtctagtgt ttcgactgca tatcttgctt caaacaagtg catacttaca
     2701 tacattattc tcatgaaaga aacaaaaata attccactta aaaaaaacaa catcaacaag
     2761 tcaacagcta atatcttggg agtggtaaga gaagttataa aaaaggttat aggaactatc
     2821 aaaatgcaaa ctatgccaaa aaatagccat tgaaaaagaa caaaaggtgt tttcccactc
     2881 aaagaatact tatatgcaaa cttagcataa tcgttaattg ctttattcaa ggctttaaat
     2941 gatgttactt tagcaccaaa tatctttata acttgaattc cgcggatata ttctaccgtt
     3001 tcagagctta acacatctaa cgattcttga tatttttgaa taaaatttgt attctctgtc
     3061 attttcttta acaagaatac accaattact aacatggcga ttagaaccaa tccaatttta
     3121 aagttaatga aaaagcctaa ggcaactgat aaaacaggtg ttactatggc ttgagcacta
     3181 tctggaatca agtgagcaat tgccatatgt gttttagccg cgttgtcatc aattgtcttt
     3241 cgaatatatc ccgaagaatt taaatcaaaa aaacgaaaac ttgcttctgt taaaccgcta
     3301 attccctttt ttctcaaatt agtctctaag cgaaacccaa ggatatgaga aaataagcca
     3361 gaaaaaatat aaaataaagc tcctaaagtt aaccaagcaa cagtttgaac cgcataactc
     3421 ttagctaaat attcatctcc tacaataatt agagactgaa gaaatttgta aatactgtaa
     3481 tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa ccatccgtag
     3541 tatttcattt tagggacata actaaataat tttttgtata ctttcatttt ttcctccccc
     3601 gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa tagcgtaaat
     3661 tagagatgta caaacagata aaaggtaaac tacgttctat tttttgtatt tttttaagac
     3721 ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca ctgaaatgat
     3781 tccccatata cctaccgcat agtggatcat tgcatccatt gttgcagcag attgtccttt
     3841 atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc actgtccggc
     3901 agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc gatcatatcc
     3961 aaatacagat agaataatat cagctaagat cgccatacta atcgaactga taaaccaagg
     4021 taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt aaatagtaaa
     4081 aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag ataataatcc
     4141 aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca atactactaa ccattattga
     4201 aaaaaagtaa attactaggg aaataactgt agctaaacca ataaaaatat aatcttttgt
     4261 tttcatatat attctcctca attctatttc gttatgtcat taaaaatctg ttctatcaaa
     4321 gttgaatctt ctactctaag ttttatatct ttttttattc caccattttt taaataaata
     4381 attctattgc atgttttata aagaaattcc ggatcatgag tagctataac tactgcaccc
     4441 ttttctttta agtctttaat aaggtcagca atcctttcca tattaatata atccaaccca
     4501 cttgtaggtt catcaaatag aaataaatta gtttttgaca atgaagctat agaaaccaat
     4561 aatctttgtt tttctccacc ggataaatca aacggtaatt gatctttcat tttataaagt
     4621 tccatttttt ttaaaatttt ttcttgctga tctttactaa ctatttcatt ccccagggct
     4681 aactcgctcc tcactgattc tgtgaaaaat tggtaatcca tttcttgcat tactaaaaac
     4741 ggcgatgaca aatttttttt attttcgatt atctttcctt tggtagggcg aataatcttc
     4801 atgagtattc ttagtaaggt tgttttcccc acaccgtttt tccctaccaa accaataaca
     4861 tctccaactt tgacatcaaa atttatgtct tttagaatat tttcataagt aatttcttca
     4921 acttttaata catctgcact taccttttcg atatagggaa cttcaatcga actattaaaa
     4981 atgtcaaatt tccttaatgc gttattgttt tcagtt
//
LOCUS       DAXLPI010000144         4858 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXLPI010000144 DAXLPI010000000
VERSION     DAXLPI010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.148"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            235..1875
                     /locus_tag="ACF3T5_002474"
     CDS             235..1875
                     /locus_tag="ACF3T5_002474"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFD3633001.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2029..3351
                     /locus_tag="ACF3T5_002475"
     CDS             2029..3351
                     /locus_tag="ACF3T5_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFD3633002.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3413..3844)
                     /locus_tag="ACF3T5_002476"
     CDS             complement(3413..3844)
                     /locus_tag="ACF3T5_002476"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3633003.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3908..4744)
                     /locus_tag="ACF3T5_002477"
     CDS             complement(3908..4744)
                     /locus_tag="ACF3T5_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFD3633004.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
BASE COUNT         1499 a          963 c          902 g         1494 t
ORIGIN      
        1 ctatttatct atctaacata tcctttattc ttctactttt ctttctttag catataagag
       61 atacgcatag taatcaagca aggattgttg atataaaaag aacaaatacc gctattttga
      121 tttcttttga ctctattact catataaata aaatttatta tacctgctct ttttgactat
      181 atcctcatct gactcctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
      241 acagggctaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      301 gaacgggcaa aatacggaat cactggtatg cttccaagta ctgtccaaac attggaacaa
      361 caatctgtcc aagcttacgg tcaatactta agcaaacaga ccgatttgga aaaacgtatc
      421 ttcttaatga atctattcaa tacgaaccgg acattgtttt ataaattgat gggcgaacat
      481 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      541 aacgaaattt tcttgaaacc acaagatgct gtatttcttt caatcgacga accagaaaat
      601 atcaaagcaa gtttgaaaaa cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca
      661 gatgcagaag gaatcttagg tatgggtgac tggggtgtga acggtgttga catcgctatc
      721 ggtaaattga tggtctacac tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta
      781 tcaatcgacg ctggtactaa taacgaagaa ttattgaata atccattata cttagggaat
      841 cgtcatgctc gtgtagaagg cgaaacttac tacgaattca ttgatcaatt cgttcaatca
      901 gctactgaat tattcccaga attgttatta cattgggaag attttggacg aggaaatgct
      961 gcaacgatct tagaaaaata cgaagataaa atcactactt tcaatgatga tatccaaggc
     1021 acaggaatcg ttgtactagc tggtgtatta ggtggattga atatatcagg tgagcaattg
     1081 aaagatcaaa cgatcttgac atttggtgct ggtactgctg gtgtaggtat cgcaaacatc
     1141 cttttagatg aaatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1201 caagtagata aacagggttt attgtttgaa gatacaaaag gattgactcc tggtcaaatc
     1261 ccattcgctc gtaaacgctc agaattcact aatgctgatg aattgacaaa cttagaagca
     1321 gttgtaaaag caatccatcc aacgatcatg atcggtactt ctacccaacc tggagcattc
     1381 actgaagaaa tcgtgaaaga aatggcggct cataccccac gtccaatcat catgcctttg
     1441 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt tgattgaatg gacggacgga
     1501 aaagctttag ttggaacagg tatccctgct gctgatgtgg aatataacgg tgtaacttac
     1561 caaatcggtc aagcaaataa tgcgttgatg tacccaggtt tagggcttgg tttgattgct
     1621 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1681 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1741 ttctctcaaa cgattgctga aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt
     1801 gaagaaatca cggatatcaa agaagctgtt gaatctgcta aatgggttcc tgaatataaa
     1861 tctttagaag attaagattg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1921 aaaagaaaaa agcgactgcg gcaaaacagc agccagccaa ctactgctct tttgccgctt
     1981 ctctttggct tttttcatac gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     2041 acaacaaccg caactgcagt tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt
     2101 accggcgtaa gtttgcccct ttatttgatc atggtccttg tcctgatcat tgcaatggct
     2161 ttaggaaaat taccgacgaa tatgattggc ccaatcgctg ttttggtcat tttcggtaat
     2221 ttatttcatt ttatcggaac aaaaatccct atagtcaaaa gttatttagg tggtggctcc
     2281 gtttttgcga tctttgcctc tgccgcgatt gctacatttg ggatcttacc tgagtatgta
     2341 gtaaaatcca cagaaaactt tgtcagcaat atgggctttt tagactttta tattgctgcc
     2401 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2461 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt
     2521 atgcttatcg gaaatggttt tgcaaattcc gttttatata tctcattgcc cgcaatggct
     2581 ggtggtgttg gagccggagc tgtaccttta tcaacgattt atgcaaatgt tttaggtacg
     2641 gacgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcctagct
     2701 atcattggtg cagcgttagt tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat
     2761 ggtaaattaa tggaaaaagg cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc
     2821 attccacaat taggtgtcgg tttgatggta tcattgactt tcttcatctt aggacaaatc
     2881 tgtaactatt ttgttcctaa agtccatgct tacgcattca tgatcattat cgttgttatc
     2941 tgtaaaatcg caaacctatt accagaatat tacgaagatg cagctatcat gttcaacaac
     3001 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttattgaat
     3061 ctaaatgtat tggcttctgc tttgacatgg caatttgttg tcttatgttt gacaagtgtt
     3121 atcgcgattt cagtagtttc aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa
     3181 tcaactatta ctgctggctt atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc
     3241 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcggcttggt
     3301 ggtgccatcg ttttgatcat ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3361 atacctgatc atcaagtatc ggcttgatga ccaggtattt tttgataaaa atttacagga
     3421 ttttcaaaaa tcttttttgc attttatcgg aatgataacc gatgcgttca taaaatcggt
     3481 gtgcttctac tctgtattct gcagaattaa gtcttattgc tgatattcct cgtcttttcg
     3541 cttcttctac gccagtcatc aaggcctttc cgattcctct tttttcagca tgtttagaca
     3601 cagcaagtgc cataatattg aacatcggat cgctgaaaat cgattcatag gactccgctt
     3661 gtacatagcc agctgctttt ttagaagtag gatcttcata gaccaagaaa aaattttttt
     3721 ctggctcctt caacagtttc ttcatttgtc tcaccgtaac ggttaatggt acatcatatc
     3781 ctaattgttc cgcattgatt tctcgtagtg cttctgcatc tcctaaacgc atttcccgca
     3841 acatccaaat caccagccaa tttttatttt tttaaatgaa tttttttatc acagtatact
     3901 ccttttttca cttaaaaaaa gacttttcag tgattttgac ttgcttgaat ccagatcctt
     3961 ctaatgtttg caagatgacg aaagcattgt aaagattgat caaaagaacc acgatgacaa
     4021 tcgcttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat cttgctcccc
     4081 acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa tcaaagcgca
     4141 taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag aaaatcgtag
     4201 atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc aacaaagaaa
     4261 cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga acccccccac
     4321 atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga
     4381 agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc acatgcttct
     4441 cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact cctccaacaa
     4501 cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg cttgatactt
     4561 gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc
     4621 ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg cctccaccca
     4681 tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa atcagaccaa
     4741 tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc ttttctttta
     4801 ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta aggaaatt
//
LOCUS       DAXLPI010000145         4816 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXLPI010000145 DAXLPI010000000
VERSION     DAXLPI010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.149"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..610)
                     /locus_tag="ACF3T5_002478"
     CDS             complement(<1..610)
                     /locus_tag="ACF3T5_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFD3633005.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKL"
     gene            837..2258
                     /gene="gndA"
                     /locus_tag="ACF3T5_002479"
     CDS             837..2258
                     /gene="gndA"
                     /locus_tag="ACF3T5_002479"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFD3633006.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            2451..3137
                     /locus_tag="ACF3T5_002480"
     CDS             2451..3137
                     /locus_tag="ACF3T5_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD3633007.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            3134..4639
                     /locus_tag="ACF3T5_002481"
     CDS             3134..4639
                     /locus_tag="ACF3T5_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFD3633008.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         1624 a          840 c          989 g         1363 t
ORIGIN      
        1 gcaatttacg cgtaggcaag acatcaatta attgtcctgt ttcgccatct gcgcaaataa
       61 agctcatttt atcttctatg gaagcgtgcg aacgaaattc atcaaccatc aatactctgg
      121 ggagaatcct cttagattgc tttggtaagt agcttttaaa ctctttcaac gtacgaataa
      181 cggtggtcaa agatacctga caatttttcg caataaaaga taaagatact ttttcagtca
      241 gtaaagaagc aattttatat ctgacatgat tcgcgattga atgtctgggt tgaacaaaat
      301 aactttgagc cgtccaatgg gttcgacagt ttttacaggt atagcgctgc ttttttaagc
      361 gcataaccaa aggcatgtga ttgtattgtt caaagcggac aatcgtttcc ttcttcccat
      421 ttttcactac aattactttc ccgtttccat ctaccacagt agaaccacaa tttctacagg
      481 tatggggagc aggtgagaaa acagcatcga cgatcaacgt ctttttcttc tgaaaggtct
      541 cgtaagagac ctctgtaatc atcaaatctt tctctattaa tcgcagcatt tttttgatag
      601 aatcattcat atagcatatc gtcctctcag ttgtttattt tgtcgtgatt taatcatact
      661 agagaacgat atgtttttta atacctaaaa taaaaatggg gctgaagaat caattctgat
      721 tcatcagtcc cataaattat agagccaaaa tatgttgttc tttagtttgc aatgtagccg
      781 tgttaagcta taatgtaaga gattagggag aaatcccgta tgtaggagga gttcatatgt
      841 caaaacaaca aatcggcgtt gtcggtatgg ccgttatggg taaaaatcta gccttgaaca
      901 tagaaagccg cggctattca gtggctttat tcaatcgtac aggggctaaa acaaccgctg
      961 tagtagaaga acacccagat aaaaatttca aagcaacata tacaatcgaa gaatttgtag
     1021 aatctatcga gaaaccacgt cggatcttgc taatggtcaa agcaggaccg gcaacagatg
     1081 caacaatcca agagttactt cctcaccttg ataaaggcga tattttgatt gacggaggaa
     1141 atacattctt taaagatacg atgcgtcgta atgaagagtt agccaactct ggcatcaact
     1201 ttatcggtac aggcgtttcc ggtggtgaag aaggagcttt aaaaggacct tcaatcatgc
     1261 ctggtggaca aaaagaagcc tatgcgctag ttgcaccaat cttagagcaa atttctgcaa
     1321 aagcagaaga cggagcacca tgtgtcacat atattggccc aaatggagcc ggacattatg
     1381 tgaaaatggt acacaacggt attgagtatg gagatatgca actgattgct gaatcttatg
     1441 acttaatgaa gaatatttta aacctttcag tagaagaaat ggctgatatc tttaaagaat
     1501 ggaatcaagg cgaattagac agctatttga tagaaatcac tgctgacatt ctgacacgta
     1561 aagacgatga aggaacaggt aaaccagtag tagatgtgat ccttgatgca gctgggaata
     1621 aaggaacagg taaatggaca agccaaagcg cgcttgactt aggtgtgcca cttccattga
     1681 tcactgaatc tgtatttgcc cgctatattt ctgcttataa agaagaacgc gtacaagcaa
     1741 gcaagatttt gtctcgtacg aacgattttg agttcacggg agacaaaaaa gaattggtcg
     1801 aaaaaatccg agaagcgttg tatttcagta aaatcatgag ttatgcccaa ggatttgctc
     1861 aattacgtgt cgcttcaaaa gaatttgatt gggacttgcc atttggtgaa atcgcaaaaa
     1921 tctggcgcgc tggatgtatc atccgagctc gattcttgca aaaaattacg gatgcctatg
     1981 acaaaaatcc tgagatcgaa aatttattgc tagatgatta cttcgttgaa atcacgaaaa
     2041 aatatcagca atcagtcaga gacgtcgttg ccttggctgt ccaagctggc gtacctgtac
     2101 cgactttctc atcagccatc gcttacttcg attcatacag agcagaacgt ctgcctgcaa
     2161 atataatcca agcccaacgc gattactttg gcgcacatac ctatgaacgt gtcgacaaag
     2221 aaggaatctt ccattactca tggtaccatg aagaataata gtctttcggc tgttaggaaa
     2281 tgagaaaata aaaagaggac gaaagagctg aagtcgattg ttatgagtag acttcagctc
     2341 ttttttaaaa ttgctatcca tgataaaatg actatagatt tttaagcata ttatcggtat
     2401 aatgatagag tttttaaaaa gccagtagaa tagaaaggat aaaataataa atgagcaata
     2461 ttctaatcat tgaagatgaa aagaacttgg cacgatttgt cgagctagag ttgaagcatg
     2521 aagggtataa cgcagaagtc cactacaacg gccgaactgg tttagatgcg gcattaaata
     2581 atgagtggga tgccatattg ttggatctga tgctcccaga attgaacggt ttagaagtct
     2641 gcagacgtgt acgccaagtc aaaaatacac caatcattat gatgactgca agagattctg
     2701 tgatcgatcg tgtatctgga cttgaccacg gagcagatga ctacatcgtc aaaccatttg
     2761 ctatcgaaga attattagca cgtcttcgtg cattgttgcg tcgaatcgat attgaaggcg
     2821 ataaaaatgt ggctaaacaa actactatta cttatcgcga tttgactatc gaaaaagaaa
     2881 accgtgtcgt acgtcgcgga aatgaagtaa ttgaactaac aaaaagagaa tacgagttgc
     2941 tgcttacttt aatggaaaac gtgaacgtgg tattagcacg tgacgtttta ctaaataaag
     3001 tgtggggata cgaaacagaa gttgaaacaa acgtagtgga cgtatatatt cgttatcttc
     3061 gcaacaaaat tgacgttccc ggggaggaaa gctatatcca aactgtacgc ggaacaggtt
     3121 atgtgatgcg ctcgtgataa agccgaaaaa atcggaaaaa agagaactga aagggccctc
     3181 cttgacaatc aagtgggcct ttgcgagttc tttcttcata ttcgttgtgt ttacaatttt
     3241 cgctgtcatt acctataaat catctatcaa tctgattgtg gcaaaagaaa gaaacaatgt
     3301 agagcgtacg atttcagaag tagcttctcg tttggctaac tcggatgaag aactgacatt
     3361 agtaaatacg tatcgtaatc tgacgaaagc gacaaatgac ggagatgagg tcgtagatca
     3421 tacttcactg gtcgaaggaa acttaatgaa gatcgattcc tttatagctg agctagggca
     3481 gccatcaatg gatctttatg tttatgattt ggacgagaac ttgattttta aaacccatga
     3541 aaacagtcga aacctgattc agactacacg taaagttcca acgatcgtta cactagaagg
     3601 aaaaactggc tttttatcta tccagcctat atattctaaa ggtacaagag aaaagatcgg
     3661 ctatgcgcaa agcttctatg aattatcttc tttctatgat attcgaaata agctattatt
     3721 gactcttatt gttttagaag ttgtttcttt gatcttaagt agtattttag gtttcttttt
     3781 atcttcttat ttcttaaaac cactcaaggt actcagagat acaatggata ctatacgtaa
     3841 agatccgcaa tcagatatcc acatgcctga aatcgacaca aatgatgaat tggcagattt
     3901 agctgaaata ttcaatgaga tgcttgaccg tatgcgtttg tatattgaac aacaagagca
     3961 attcgtggaa gacgtatctc atgagttaag gacacctgtt gcaattattg aagggcatct
     4021 tagtctgcta aatcgttggg ggaaagatga tcctgagata ttagaagaat cattaagcgc
     4081 cagcatgcaa gaaattagcc gtatgaaaag cttagttcaa gagatgctag acttatcaag
     4141 agcagaacaa gtagatgttc attatgcgaa tgaaacaact aaaggaaaag aagtcaccta
     4201 tcaagtttat aataatttca agatattata tccagactat gtgatcactt tagatgatga
     4261 tttgacaaaa gaagtgaccc tgcaaattta tcgtaatcac tttgaacaat taatcattat
     4321 catattggat aacgcagtaa agtattcaac tacacgaaaa gaagtccata tctctatttc
     4381 ttccactctg aatgaatttg agatcgcgat tcaggacttt ggcgaaggaa tacccaaaga
     4441 agaccttaat aagatcttca atcgttttta tcgagtggat aaagcaagag ctagaacgaa
     4501 gggtggaaat ggtctggggt tgtctatcgc taaacaactg gttgaaaact ataaaggacg
     4561 tatcttagca gaaagtgtag tagggcaagg gacaattttt agaatcttta ttcctatcgc
     4621 aaaggcagaa gacaagtaaa gcatgtgctt tacttgtttt tttatatatc caacaaagaa
     4681 taaggtacaa aatacaagca agttagaaat tcagggatgt cattataaaa aaaactagcg
     4741 aacaattaaa tagaataaca tccgttaatt aaacgtaagt agcttttaga tgaacgaaaa
     4801 aacaatgaaa tgctgt
//
LOCUS       DAXLPI010000146         4753 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXLPI010000146 DAXLPI010000000
VERSION     DAXLPI010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.150"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            137..481
                     /locus_tag="ACF3T5_002482"
                     /pseudo
     CDS             137..481
                     /locus_tag="ACF3T5_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            512..832
                     /locus_tag="ACF3T5_002483"
     CDS             512..832
                     /locus_tag="ACF3T5_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFD3633009.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(861..1325)
                     /locus_tag="ACF3T5_002484"
     CDS             complement(861..1325)
                     /locus_tag="ACF3T5_002484"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3633010.1"
                     /translation="MVTYNWIKLREHPELVKQSAKWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(1454..2703)
                     /locus_tag="ACF3T5_002485"
                     /pseudo
     CDS             complement(1454..2703)
                     /locus_tag="ACF3T5_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            complement(2732..3049)
                     /locus_tag="ACF3T5_002486"
     CDS             complement(2732..3049)
                     /locus_tag="ACF3T5_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFD3633011.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(3075..3395)
                     /locus_tag="ACF3T5_002487"
     CDS             complement(3075..3395)
                     /locus_tag="ACF3T5_002487"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFD3633012.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(3796..>4753)
                     /locus_tag="ACF3T5_002488"
     CDS             complement(3796..>4753)
                     /locus_tag="ACF3T5_002488"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFD3633013.1"
                     /translation="DRYGDRVKNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNY
                     HKVLATAKAVEIYRSGNYGGRIGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDP
                     MVKGYYTEEFLELCQKQSIYFDPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNE
                     ETPFHPERYYEVFDLPGKKMNNSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGR
                     EHEERYMDEKGIVQDDYRIEFIGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFK
                     NRYGLVRIELNDERSRTLKKSASWYQTLIDKRELDLPDEIYK"
BASE COUNT         1462 a          992 c          790 g         1509 t
ORIGIN      
        1 ttcgctagag tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg
       61 tgaatgtgtt gtggtaactc tattctacag aagggacttc cttttttcta tttacacaaa
      121 atattttaca ctctcagaaa aagcgaaaaa tttagtaagc ttacaaaacg gcacaatctc
      181 tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag taactggtgt
      241 ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg atatcgctgt
      301 caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgaaa tgattgcaga
      361 aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag aagcagcaca
      421 agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt tgaaaccttg
      481 aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa atacccatga
      541 gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc gcttctattt
      601 ccaaaatcgt tacacattga tctcactggc tgtcgattta ttaaccggga ttttctatat
      661 ttttggaagc cttgcttcct taactaaaat ccccgatcga tatggcacct atttttattt
      721 ggctggtgct attttcttga cgattcgtcc tattctcaga atcgtaagga acatttttat
      781 ttacgacgaa aaaaaagcaa tgaagagaac aagcaaaaaa aaagaggcat gagatttgaa
      841 aaaatctcat gcctcttttt tcaccctatc gtatcacttt gatacagacg agcggtcgtc
      901 ccactttctt ccttgaccat tgtcaaaaat gtccactcgt atcgttcata aaacgtggta
      961 tgatctgtaa ttaagtaaag tttttcgtac ccaagttgat gcatttcttt tctgatttcc
     1021 gctaatatcc gcttggctat cccctgctta cgcttttccg gttcaacaaa cagggcacaa
     1081 agatttggtg tcaaatcagg gcggttatgg aagtcgtttt caataacgcc cgctcctccg
     1141 acgatggttt ctttttcatc taagaccaca taccattgag gaatactttc cttattagcg
     1201 attccagtac gaatactttc ctcatacaca acagcaggaa ctccccattt tgatgaaaac
     1261 cacttcgcac tttgttttac taattctggg tgttctctta atttgatcca gttatatgta
     1321 accaatgttt aaacctccga ctaaaataaa acgtttacaa aaattataca agtctttatg
     1381 aaaattagca atgacaacaa tagtgatgta aaaaaaacgg aaaacaatca cttgttctcc
     1441 gttttttgat taatcaagca tctaaagctt cttttcttct ttcttggatc gtagctacct
     1501 tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt gacagcggct agtacaccac
     1561 ctgcaatact ttttgttgcc aatacaccgc caataattgg tggagttacc catggaggca
     1621 taacggtagc tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga
     1681 ccattggtgt caaaataaat ggaatgaaca tgatcgggtt caacacgata ggtaacccaa
     1741 acatcgttgg ttcattaata ttgaagacac ctggcgcaat cgacaagttt gttacaacca
     1801 tatatggttt gtttttacgt cctactaaat aaatagctaa cagtaaacct aaagttgcac
     1861 ctgttcctcc taagttgaca aagctgtcaa agaaaggttt gttcacgata aacggtgcgc
     1921 tatgtccagc tgcgattgca ttaacgtttg cttcaatggc tggcgcattg attgtttgca
     1981 tcaaaggatc gaccatattc gctccgtgta aaccaaagaa ccataagaat ggtgtgataa
     2041 atgcaagcag taaggcagat ggataagagt ttgcaagtcc cataaatggt tcttggacca
     2101 ctgtatagaa agaacctacg atatcatcaa caccgaatgc agcaaaaatt gcagcaacta
     2161 aagcaaacgc agagatagtg atcattgctg gtagtaatga agcaaatgct tttgcaactg
     2221 ccggtggcac accatcaggc attttgatga tcaatttttc atttccgctt aaacgtacaa
     2281 agatttctgt tgaaataatc ccaacaataa tagcaataaa taaaccgttt gagctcatac
     2341 ctaatgctcc gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt
     2401 cttttcggta gccgttagct aaatgataag caagaaagca atcaagacag aaagaatggc
     2461 aaatgtaccg ttccaagcag cagccccgaa tcctttccat gattcaccac caaaaattga
     2521 gttcatcaat tcttggaacc cgggaatcgg taagttattg atcattactg ctaatgctcc
     2581 taagatcatt aacggcatac tcaccataaa tgagtcccga atcgcaacta aatgccgttg
     2641 tgcaccaatt tttgatgcta caggtataaa atgtttctcc ataaatgcaa caaatttatc
     2701 cataaatctt ccctcttttc tttactgtag attacgcgtt caatcgacga tacagatcaa
     2761 tgatctcttt tgctaaatct gtaaaagcta tggatgtcat cagatgatct tgactgtgga
     2821 ctgtcaaaag cgtgacttca acgtgatttc cttgtgcttc ttgtgtcagc atctctgttt
     2881 gagagtgatg tgcttggact aaagattcct ctgcatcttt gattttttca tctgctaaag
     2941 tgaaatcacc ttttttcgca gcagcaatcg cttccattgc atcgcttttt gcatttccgc
     3001 caaacatgat cagacccatg atggcttcta gatttttttg atcttccatt tattgtatcc
     3061 tccgataatt tttattattt gtttattagt ttttctgctt ggtcgagtac tttttcacca
     3121 ttcatcatgc catagtcagc catattgatt atatctaaag gaatgccttt aggtgctagc
     3181 ttttcagcaa attgattttg cataaagcgt acttgcggtc ctaaaagtaa aacgtctaca
     3241 tttttagatt ctagttgatt atccgcatct gaagcagata cagcaaagat gtctgcttcc
     3301 attccttttg cttctgctgc cttttgcatt tttgtgacta ggagacttgt actcattcca
     3361 gctgaacata caagcatgat tgttttcttt tccattttaa acacgttcct ttctatactt
     3421 acacttgtat ataagcaagt agcgtgccaa ctttgaaagc gctttaaaaa gttgatatca
     3481 tcacatttaa ggtctatacc aagttgcata cacactaaaa aagaaagtgc acactaaaca
     3541 cacaaattaa tttttattac cttttttctt ttaacgtgca caaaaaaagt tgtgacacaa
     3601 aacttgtcac aacttttttt cgaataaacg gtattcaaaa gcttgcatct gcggtaataa
     3661 gtccaaccaa gttaaaaaca taagatgttg tttttacttt gctattctta ttacttgatg
     3721 caggacagct ttttcacaac ctcttttatt tcaaataacc agtaaataca atgaacatct
     3781 acaaaaaact tctatttatt tatagatttc gtcaggcaaa tccaattctc tcttatcaat
     3841 caaagtctga taccacgaag cagatttttt caatgttctg cttcgttcat cgttcaattc
     3901 gattcgaact aatccatatc gattcttaaa tgcattcatt ggtgacacac agtcagtgaa
     3961 tgcccataac atatagcctt cgcaatttgc gccttcttct gttgctttga tcaaataacg
     4021 caaatgctgt ccgataaatt cgatacggta atcatcttgc acgattcctt tctcatccat
     4081 ataccgttct tcatgttctc ttcccatacc gttttctgtt atcagccaag ggatattatg
     4141 ataattttct tttagataca tggctatatc atacatgatt ttcggataga tttcccatcc
     4201 cctggaatta ttcattttct tcccaggtaa atcaaagact tcataatatc gttctggatg
     4261 gaaaggtgtc tcttcattcc attgataacg aggagcgcga acgcgtttag gataatattg
     4321 attgattcca aggaaatcta ctgtatgatg ctgaatcgtt tctaaatcct ctgttgcagg
     4381 atcgaaataa atcgattgtt tttgacacaa ctccaggaac tcttctgtgt agtacccttt
     4441 taccatcgga tcaaaaaata ctcgattata aaacaaatca tacatataag cagctttttg
     4501 gtcttctgga gaattagatc gagcatagac catttcagga tttaaaatac agccaatcct
     4561 tccaccataa tttccgctgc ggtaaatctc tactgcttta gccgttgcta aaactttatg
     4621 ataattccac atcatccact tttttgtgtt ttgttcatgc ggccagcgaa cagcatccaa
     4681 atagcaacgc gtttggggaa cgattggttc gttgaatgta aaccagtttt ttaccctatc
     4741 accgtaacga tca
//
LOCUS       DAXLPI010000147         4743 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXLPI010000147 DAXLPI010000000
VERSION     DAXLPI010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.151"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(289..2955)
                     /locus_tag="ACF3T5_002489"
     CDS             complement(289..2955)
                     /locus_tag="ACF3T5_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFD3633014.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            3297..>4743
                     /gene="cls"
                     /locus_tag="ACF3T5_002490"
     CDS             3297..>4743
                     /gene="cls"
                     /locus_tag="ACF3T5_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFD3633015.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPI"
BASE COUNT         1385 a         1017 c          859 g         1482 t
ORIGIN      
        1 tcttttgatg aataataaaa tgattggctt tccgtctatc ataaataaaa aaagggactg
       61 tgaccaaagt cgtgtcaaag tccctctttc cgaataaaca gtgtccaatc agatgaccct
      121 cagtattagc tcaaaatgct gattttacaa cttcttcctt aaacggtgtt ctcctgccaa
      181 ctcttcggtc acaagtcaca atattcaaca aacatgagaa gctgtttgtt gaaattgcac
      241 cttgtgactc agaattaaac ggcaggctca caacctcgtt gattcgtttt atttatttcc
      301 ttgtactcgt acaatgatca atttagtgat ttccatcaaa acaattgtca caagtgcgat
      361 tcccatacat gtaaagactt ctgtcaatcc aaatgctgat gggatagaga agacttcttt
      421 cagtcctggg atcattgtta agcagtaaag cgcgctacat acaactacag ctagtaagac
      481 acttctgtta gagaagaacc ctgcttggat agctgtttgt gtgtttgaac gtgctgggaa
      541 tgtttgcaat gtccgacacc aaatcaatgt tgaaaatgac atggctacac cgaattcagg
      601 tgaatgctgc atgccaagcc attgagcaaa gataacagca atcccaatca gcacaccacg
      661 atacgttaca gaaatcaatg ttttgtccgt aaagattcct gaatttggat ctcttggcgg
      721 gcggttcatc gtagcaggtt cagctttttc tgtacctaat gcaattgctg gcagtgaatc
      781 attgactaaa ttgatgaaca acagttgtaa tgctgtaaat ggattggacc agtcagcaac
      841 taaggcaaag atgatcgcaa tgatcgcacc taaatttcct gcaaatagat aagaaatagc
      901 tttcttgata ttatcaaaca cattacgtcc tacttctact gcgctgacaa tagaagcaaa
      961 gttatcatcc gtcaatacca tcgctgctgc atcttttgcc acatctgtac ccgttcccat
     1021 agcaattccg atgtctgctt gtttcaatgc tggtgcgtca tttacaccat cacctgtcat
     1081 agctgagata tgacctttat tttgccaagc acgaacgata cgaattttat tctcaggtga
     1141 aacacgtgca taaacagtga ctttttctaa aacttcgttt agttgatctt ctgtcaattc
     1201 atctagttcc gtcccggtca aagccaggtt tccttcttca aaaatcccga tttgttttgc
     1261 gatagcaaca gctgttgttt tgtggtcacc agtaatcatg attgttttga ttcctgcact
     1321 tcttgcttct gttaccgcct gagtcacttc agcacgtgga ggatcgatca tagcaagcaa
     1381 accaacaagg atcaaatcgt cttcatcttc aaatgataaa tcaaaattat caagtggacg
     1441 gtaagcaaaa gctagtacac gtaatgcgcg tgttgaaaaa gcttcatttt gctcttggaa
     1501 ttttttcatc aattcatccg tcatcggaac gacttcacca tttagcaata cttttttact
     1561 gcgttggaag acgatatctg gtccgccttt gacaaacaag taattttcgt tatcgatcgt
     1621 gtgtccagta gacattaatt ttcgatcaga atcgaaagga agttctgctt gtcttgggta
     1681 gttgtttcgg atttcctgat aaggctggtt taatttttca ctaaagtcga taaacgctac
     1741 ttccgtaggg tcgcccaatt tagtaccgtc ttcactgatc gatgcatcat tcgctaaaac
     1801 actgatttcg atcaagcgac gttcgtcagc tgtccatttt gaagggtcat ttgtgaaatc
     1861 cccagtatcg ccattaccta aaaagtagtc aacaactgtc attttgtttt gtgtcaatgt
     1921 accagtctta tctgtacaga tgatacttgt cgaaccaagt gtttcaactg ctggaagctt
     1981 ccgaataatg gcatgacgtt ttgccatttt attcgttccc aaagataaca cgattgttac
     2041 gatagactgt aatgcttctg gaatagcagc tacggctacg gctacggcaa acatcactgc
     2101 gctgacgata tctgcagtca tatctcctgt accttctcca agataaatac gaaacagttg
     2161 gatacctaaa atcaataaag acaatgccaa aatagccaga cttagttttt tactgaattt
     2221 atccagacct cgttgtaacg gagtgacttg ctcagttgtt gattccaaaa ggccagcaac
     2281 ttgtccgatc tccgtattat tcccagttga agtcactaag aatttaccgc gtccgtaagt
     2341 tacgatcgtt ccactgaata ccatatttgt acgatctccg attggtaccg tacttgtgat
     2401 atccgttact tctttttctg cagaagtcga ctctcctgtc agcatacctt catctacttt
     2461 taatgatccc gcatctagta aacgtccatc agcaggtaca taatcgcctg cgtctaacaa
     2521 cacgatatct cctaccacaa gttcgtttgc cggaatcgtt ttttccttac catcacgaat
     2581 cactcgagca tcgggcgctg aaaggttttt caacgcttct aaagaacctt ctgctttttt
     2641 tgtttggaca acactgatta tagaattcaa catcaatact gcaaaaataa ctaagaattc
     2701 gatgtagtca cccatgacca tctgaatcag tgcgacaatc agcaatacga taaccattgc
     2761 atctttgaaa gtttctaaga acaatttcca agttggcttc ttcggctttt cttttagctg
     2821 gttcaagcca tctctttcta atttttcctt cgcttgcgtg gtgctcaatc catcggttga
     2881 gctctgcgtt tgctgcatca acgagtcgat cgtttcttta tagaccgctt gttgtgcttg
     2941 ttccttgttt tccaaatttc atcccccatt ttttgatctt ggccctagtc ctgtctgaaa
     3001 aaaaaagaga cctaagacaa atacgcattt gtcttaggtc tcaccatttc atacaatacc
     3061 agaaaaccat ttggttaact tactgttgat attatcgcaa caaacgttgc cggttactcc
     3121 cctaaggaga tattcaactg tactaatatc ctaccttatt aacctacaaa aagcaactac
     3181 tttagcccga tttacctaca ttttcatgaa aggagtgaaa agtatattca ccttggtagg
     3241 cttctttctt ctatcatgct atactaatgt tattgtatgt aaggaggaac atttttgtgg
     3301 tatctagtat tataaccgcc ctttatctat taaatgcact tattgctttg gtggctattt
     3361 tgatcaaacc ccgagatgta gcagccattt gggcatggct cttagtattg tttgcccttc
     3421 ctggggtggg ctttgtatta tatttatttt tcggacgtgg attaacggat aagaaaaaat
     3481 tctatctccg acaaagtgac ttgaaagaat tagaaaactt tcagtctttt agaggagata
     3541 ccattgaaca ttacgatcct gacatgggcg ataaagacaa acagcaattt gtcgacttct
     3601 tctcttcatt aaatcgtatg ccgctgacaa gaatgaattc tgtcactctt ctcacagacg
     3661 gacaagagaa attggattca ctgcttcaag atctaaaaaa agccaaacat tcgatccata
     3721 tcgaatatta cgcatttgtg acagataata tcggccagca agtcttacat gttttagaag
     3781 aaaaagccgc agaaggcgtg gaagttcgaa tattatatga tgcatttggc tctcatggta
     3841 caaaagcaaa agatttcaat cgtctaatca aaaatggtgg acatgtccat acatttgtta
     3901 cctcacaaaa ggcattactt cgtttccgat tgaattacca tgatcaccga aaaatcgttg
     3961 tgatcgatgg aaagattagt tataccggtg gtttcaatat tgccaatcaa tatgtaaata
     4021 caacaaaaaa attcggctat tggcgcgata cgcatatacg gattttcggt gccgcttctt
     4081 ctttgctcca gcttcgcttc ttaacagact ggaacgtctc ggtacctgaa gaaaaaaagg
     4141 tcggctatca tttgaattat ttctttaaaa aagcagatcg agatgaatct aagcttgctg
     4201 atacatccat ccagcttgtt tcaagcggac cgaataacga aagggaacaa atcaagcttt
     4261 catttatcaa attgattact tctgctaaaa aacgtgtttg gatacagaca ccttaccttg
     4321 ttcctgatga aagtgtcatt gctgctttaa aaatcgcaac tgcctctggt gtagatgtga
     4381 aaatcatgat tcccaacaaa ccggatcatc cttttattta tcgagcaaca caatattatg
     4441 ctcggcagct gatcaaggaa aatgtacaaa tccttgtcta tgagaacggc ttcctccatg
     4501 caaaaacatt gataatggat gatgaaatct gcatggtagg ttcagcaaat caagatattc
     4561 gtagctaccg attgaatttt gaaacaagtg ctgtcattta cgatcctgag tttttagaag
     4621 aacttgctac tcagttcaaa gaagatgaga cacattgttc atccatgaca actgaaacag
     4681 tcaaggaaat gtctaactgg ctattattca agcaacaaat ttctcgatta ttttctccaa
     4741 tcc
//
LOCUS       DAXLPI010000148         4738 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXLPI010000148 DAXLPI010000000
VERSION     DAXLPI010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.152"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..1119
                     /locus_tag="ACF3T5_002491"
     CDS             <1..1119
                     /locus_tag="ACF3T5_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFD3633016.1"
                     /translation="LFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1247..2623
                     /locus_tag="ACF3T5_002492"
     CDS             1247..2623
                     /locus_tag="ACF3T5_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFD3633017.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3024..4604
                     /locus_tag="ACF3T5_002493"
     CDS             3024..4604
                     /locus_tag="ACF3T5_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFD3633018.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
BASE COUNT         1534 a          789 c          988 g         1427 t
ORIGIN      
        1 ttgtttgaaa aacttagaca gtctaaacta atgttttggt cgcttgaatt attgatcata
       61 gctgcattga tcatgatttc atcagaaata aactttattt tcgaaccgat cgggacattt
      121 ttttccacgt tatttgcacc tgtactgatt gcaggctttc tttactattt attgaatcct
      181 attgtcaagc tgttgatgaa agcaaaaatc aaaagaatat gggctgtcgc aattgtgctg
      241 ctccttttag ttgcagcaat tatttggatt ttacttagtg tcatcccaag tctagtacag
      301 caaatttcct cattggcatc taatatgcct gatttcatca aacaggtaga aagctggtta
      361 aaagaggtag ccgaattgcc tttgttcaag gaagtagata tcaataaata ttttgagcaa
      421 ttagacattt cttacgggac aatcattcag caattcctta gtggagtatc caatagttta
      481 ggttcgatcg ttggaacgat cgcttcggca accgtagtaa tcattacagc accattcatc
      541 ttgttctata tgttgaaaga cggagaaaaa ctagtgccaa acatccaaca gtttttccca
      601 gaaaaaagaa gaaaccaaat tgtggagtta ttggggcaac tgaataaaac attggctaat
      661 tacattagcg gacaagcaat cgaatgttta ttcgttggta ccttcacatt tttaggatat
      721 atggtaattg gtgtggatta tgctttctta tttggggtca ttgcgggatt gacaaaccta
      781 attccatatt taggacctta tctaggacta gcacctgcat ttctagtaac agtcttcaat
      841 gatccggtaa aagcattact ttgttgtgtg gttgtgctaa tcgtccaaca gttggatggg
      901 aatatcattt atcctaatgt aattgggaaa tcactgaaaa tccatccatt aacgatcatt
      961 atcgttcttc tagtagctgg aaatattgcc ggattgcttg gtatcttctt aggtgttcct
     1021 ttttacgcaa tctgcaagac gatcatttcc tatataatca aaatcgtcaa agaagacaag
     1081 caaaatgaaa acaagaagaa aattgctacg acctcgtagg tagttttagt acacttgttt
     1141 ttattgcaaa gaaaattgct ttgtgatacc attttaacgt ggctatagtg ccacgttttt
     1201 ttctatcgta aactaaaaat taattttata aggagagaat gcgattatga atgctgaccc
     1261 tgagagtcag tcgctgttag cgcagatttt attattgatc gttttgacat tgatcaatgc
     1321 ttttttagca gcttccgaaa ttgctgttgt ttcgatcaat aagaatcgaa tcgagcaaaa
     1381 agctgatgaa ggagatgtga aatctcaaaa gcttttaaaa atattgcaga atccaaataa
     1441 tttcctatcg acaatccaag taggaatcac tttggtcaac attttgtctg gtgcttcact
     1501 agctaatact ttatcggaac ggttagctcc ggtattaggt ggcggcacag cggcaaaaaa
     1561 tattgcaaat atcattgtgc tggctatttt gacctatgta tcgattgttt ttggagaatt
     1621 gtaccctaaa cgtattgctt taaataaatc agaagaagtc gcaaagttca cttcagggct
     1681 gatacgcttg attggtgtag tagctaagcc cttcgtctgg ctgctttctg cttccacaag
     1741 tttattggcg cgtatcacgc cgatgacttt tgatgatgaa gattcaaaaa tgaccagaga
     1801 tgaaatgcgt tatatgcttg aaaacgaagg cgtattgaat aatgaagaat tagaaatgct
     1861 ccaaggggtt ttttctcttg atacgaaagt tgcccgagaa gtcatggtcc cacgaacgga
     1921 tgcatttatg attgatatca acgacactgt ggaagaaaat gtgaatgaag ttttatccga
     1981 aaactattca agaatccctg tttataacga agacaaagat aaagtagtcg gaatccttca
     2041 tacaaagaat ttactaaaag ctgcccataa attcggcttt gataatttag atatcaagaa
     2101 aatcatgcag gagcctttat tcgttcctga aacggtcttt attgatgatc tgttatatga
     2161 aatgaaaaag acgcagaatc agatggctat tttattagat gaatatggcg gcgtcgttgg
     2221 gttagcaaca ctagaagacc ttttagaaga aatcgtcgga gaaatcgatg atgaatcaga
     2281 tgaagttgag aatctttatg aaaaaatcga tgaacacgaa tatatcatcc aaggacgtat
     2341 gctgattgat gagttcaatg aagcttttga cagtgatctg catatgagtg atgtagatac
     2401 gatggctggt tatttgatta cagcattagg catgattccg gatgaaggcg aaaagttatc
     2461 gtttgatgtc gataatatta cgctggtttc tgaagaaatg gaaggttctc gaatccttaa
     2521 aatccgtgtc atttttcatg atccggaaga aacagaagcg gaacctgaag aagaacgaag
     2581 atacttcaga aaagaattcg aagatgacga gccaagaaga taattggaaa cttggttatg
     2641 aaagacgtgt tgcttgaata aacaagcaac acgtcttttc ttttagtcag ctgacaaaaa
     2701 aatatttgac tcttatggtt tggtggcttt ttcattcgct gaatccatgc tatactgatt
     2761 cagttgtagt agttgacaaa ggttgtgaaa aagctatcct gcattaagta ataagagcag
     2821 ccaagcaaaa acagcttttc gtatttttgg cttgggtggg cttattgctg ataggctgtc
     2881 tctagaacgc cgtttactcg gaaaaagaga ctgctacacg gcttttgtca cagccctata
     2941 tgaaattgtc tttctttaag agatcggaca atctgtgcct agagaaaata taaataattg
     3001 aatggaagaa atggagttag gttatgaata atccaaaatt aaagcaacaa gtggacaatc
     3061 gcagaacttt tgcgatcatt tcccatccgg atgctgggaa aacaacgatt acagaacaat
     3121 tactattgtt tggtggggct atccgtcaag ccgggaccgt aaaaggcaaa aaaacaggta
     3181 attttgccaa atcagactgg atggaaatcg agaaacaacg tgggatttct gttactagtt
     3241 ctgttatgca gtttgattat caagggaaac gagtaaatat cttggataca ccaggacatg
     3301 aagatttttc tgaagatact tatcgtactt tgatggccgt ggatagtgcg gtcatggtaa
     3361 tcgatagtgc aaaagggatc gaagcacaga caaagaaatt attccaagtt gtaaaaaaac
     3421 gtggcatccc gatatttact tttatcaaca aactagatcg tgatggtcgt gaaccattgg
     3481 aattgttaga agaattagaa gagctattag atatcgaatc ttatccgatg aattggccaa
     3541 tcggtatggg taaagggcta gaaggtcttt atgatatcta ccataatcgt gtagagtttt
     3601 atcgtccgga aaattatcag gatgaacgat tagtagaatt ggatgaagat ggactgcttc
     3661 ccgaaaatca tcctctaact gaaaattcct tatatgaaca agtcttagaa gaagtagaac
     3721 taatcaaaga ggctggagat gcatttaatc aagaaaaaat tgctcgtggc gatcagacac
     3781 cagtattttt tggttctgca ttgactaact tcggtgtaca gactttttta gagactttcg
     3841 ttgaattggc accagctcct tatggacaca aaacacaaga tgatcagatg gtaagtcctt
     3901 atgaggaaga attttcaggt ttcgtattca agatccaagc aaatatgaat ccagcccatc
     3961 gtgaccgaat cgcttttgtc cgaatctgtt ctggaacatt cgaacgagga atggatgtct
     4021 ggttggaacg tacaaataaa aaactgaaat taagcaatgt cacacaattt atggcagatt
     4081 cgagagaaaa cgtcgaaaaa gcagtagcag gcgatatcat cggtgtatat gatacaggca
     4141 attatcagat tggggataca ttgttcgaag gaaaactaaa agttgcttat gaagaactcc
     4201 cttcatttac tccagagctg tttatgaaag tgactgcaaa aaatgtcatg aaacaaaaat
     4261 ccttccacaa aggaatctat caattagttc aagaaggagc gattcagctt tataaaacat
     4321 atctaacgga agaatatatc attggagctg ttggtcagct acaattcgaa gtattccaat
     4381 atcgtatgtt aaatgagtat aacgccgaag taatcatgtc tccaatggga cacaaaatcg
     4441 ctcgctggat cgatccagaa gatttggatg agaagatgag ttcaagtcgc aatatcttgg
     4501 ctcgtgaccg tttcgaccaa ccgttgttct tattcgagaa ccaatttgca gaacgctggt
     4561 ttgcggataa atatcccaat gtaaaattaa aaagtttgat gtaaagaagt cttgacacaa
     4621 gctgccctgc atcaagtaat aagaatagcc aaacaaaaac agcctctcat attttgttta
     4681 gagccgtgac ggattttccg tgacggctct ttttactata ttaaaatcat agaaatgt
//
LOCUS       DAXLPI010000149         4695 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXLPI010000149 DAXLPI010000000
VERSION     DAXLPI010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.153"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(184..1503)
                     /locus_tag="ACF3T5_002494"
     CDS             complement(184..1503)
                     /locus_tag="ACF3T5_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFD3633019.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1760..2230
                     /locus_tag="ACF3T5_002495"
     CDS             1760..2230
                     /locus_tag="ACF3T5_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFD3633020.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            2452..3555
                     /locus_tag="ACF3T5_002496"
     CDS             2452..3555
                     /locus_tag="ACF3T5_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFD3633021.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            3776..>4695
                     /locus_tag="ACF3T5_002497"
     CDS             3776..>4695
                     /locus_tag="ACF3T5_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3633022.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVF"
BASE COUNT         1548 a          814 c          980 g         1353 t
ORIGIN      
        1 aattctttgg cacattttat tgaaatatgt atcactaata tccctatcaa ctgattttga
       61 ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg aggaataaat tttgggtgta
      121 aggcattttg agcatacttc tccctggata acttcccagt aaaaaaatcg ttagattgtt
      181 ggattagatc cattgactgg ctcgcctcag gcgagcaaag accctgtaga attcattctg
      241 atacacatga taactagata atttgagata gactcgtcta cccgtttgga ctaatttccc
      301 agcaactttc agaaacttca atcgaataga atgaatggtc attccctttt ggacttcttc
      361 aaagccaatc gttcttaaga agttgactaa gttgtaagct atcaagctga gcatcattcg
      421 gacatgattc tccaaaaagc gaggactgtc tgttttgtca aagtaaaagc cagctttcgc
      481 ttctttaatg aagttctcca ttgtgccacg tttggcatag atagaaaaga tggtatcagg
      541 agaaacattt tctgatagat tcgtcacgat aaattcatgt cgaaagagta gttcgcccgc
      601 ttcacgtgtt gagcgtatac acacgcgacg actttgtgac caggattgtg cttgataagt
      661 ggtcgagaag tactgaactt ctcgttcttc ccacttttga ttatcgccat aaagtactga
      721 tttctcagct atttgaccta gtctacgatt attcttcagt cgaataacat aatgactctt
      781 ttttgattca cacgaatcat acacaccagg tgtagcgaac ccgctgtctc cacgaaccaa
      841 gatgtcagtg tttggtagag agtgattata gtgctctaat aaaggtgtga gaaactcctt
      901 tacgcctttt gatgtatatt gatttcctga acgtagttca gcttttaaga agtcaccagt
      961 caatccgtca aaagctacca atggatggta accataggtt tggtagtgtg tattataatt
     1021 cgtttgttct tggtgaccaa atgtatctga atgggtcgaa tctaaatcaa tgattaattc
     1081 cgtatcatta cggatgaggc gtgctttatc gataagttct tggttcaaag cttgaagttc
     1141 atggatattc tcctcagata gtcgatctaa aaatcgggaa agtgaagatt gagaagcgag
     1201 ttctttccta tctaaaacag ctttaaacac aggatcttgt ctcaggagat tagctgcaga
     1261 atcagctgag taaccagcaa ttaattgcat aatgaactgc tcaagtatgg ataagttgtc
     1321 atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt tgcttagcca gttcagaaaa
     1381 accaaagatg ttcatcagct ctttcactag gacgagaccc gaatcactcg ataattgacc
     1441 acctgtatga gaaatagtga tatttgaatt gaattttact tggtttttgt gtaagctagt
     1501 catgaaaaga actcctttct tttggttggt ttagtcactt taaccatagc agaaaggggt
     1561 tcttttttca tcacttaacg ggtgaagatg aaaaagtaat tgtaagctgc cgtgaggcag
     1621 tttcacatgg ttttaattaa aagtatgaat tattcaggta gaatctctga agaataaatc
     1681 acgtgatcgt gacgataaaa tacacttacg tcaggtgata aaaaatacat aagtagaaaa
     1741 ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc agaaaaagcg aactatcaga
     1801 aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc tatacattaa agcttggtgc
     1861 caacaaagcg ataatctctg agttgacagt aggcggtctt gtggacagtc tggcgcttgc
     1921 tgaaggtttt attggagaag atatttatga gatgttttca gaaatccgtc agttaaaaaa
     1981 aggggaaaac tgcatagagg ttcatcatga gagcccgaat gtgagattca aacgtggtga
     2041 taatgagatg atcttcaata atcatgtgtc cgatcacacc gagctatatg gaatcgtgaa
     2101 tcaaatacta actgagcttt ttggattggg accttatgcg ttcaaggaag aaaacggtaa
     2161 tccaaaatta aacgaagaat ggcaagaaac gattgaaata caaagaaaca acccgcactt
     2221 atttcaatag gcatccacag tatttcgtaa aagggacttt gacacgactt ttgtcaaaat
     2281 cccatcgttt tttatctatt tgtgttctaa acatttcttt cagagtagta acaactggaa
     2341 tgactgaaat gtaaacagat tatttgcctg tatcaatacg gaagagtaat ataaagtgtg
     2401 taaacgctat catattattc attcaatttt ttatagaggg aaggcattga aatggaaaaa
     2461 acgtatcaag caggtacaaa tgaaggaata gtggatttta tcaatatgga agatttagaa
     2521 atagccgctt cacaagtgat tccagctggt ggttatggtt acatcagcag tggtgctggc
     2581 gacttattta cttatcaaga aaatgaacgg gcattcaatc atcagctgat tattcctcat
     2641 gtattaagag atgtagaatt acccgataca actacacatt ttgacaaaga aacgctgact
     2701 gcaccaatca tcatggcacc agttgctgct catggcttag ctcatgtgaa ggcggagaaa
     2761 gcttctgcaa aaggtgtagc agattttggg acgatctata cagcaagttc ttacgcttct
     2821 tgtacattag aagagataag agaagcaggc ggggagaaag caccgcaatg gtttcaattt
     2881 tatatgagca aagatgatgg gatcaatttg gatattcttg aagtggccaa gcgtaatgga
     2941 gcaaaagcaa tcgtattgac agcggatgca acagttggcg gaaaccgtga aacagaccgc
     3001 agaaatgggt ttaccttccc actgccaatg ccgatcgttc aggcttatca atcaggagta
     3061 ggccaaacga tggatgctgt gtacaaatct tccaaacaga agcttagtcc aaaagatgta
     3121 gaatttattg ctgctcattc tgatttgcca gtctatgtga aaggtgtcca gtcagaagaa
     3181 gatgtctatc gttcattaga atctggtgct ggcggaatct gggtatctaa tcatggtggc
     3241 cgtcaactag atggtggtcc agcagcgttt gattcgttgc aatatgtggc agaagcagta
     3301 gataaacgtg taccaatcgt gtttgacagc ggtgtacgac gcggacagca tgttttcaaa
     3361 gcaatcgcat caggtgcaga tcttgttgct attggcagac ccgttattta tggattgtct
     3421 ttaggaggaa gtacaggagt ccatcaagtg tttgatttct ttaaaacaga attggaaatg
     3481 gtcatgcaat tagctggaac acaaacagta gaagatatca aaaaaataaa attgcgtgag
     3541 aatcgtttta tttaagaaac aaatgacgaa aaaaatagga gtgcggcaga tgatgccgta
     3601 ctcctatttt tatagttcaa ggatgtcact aatagaataa tttgaaacga aaaaataaaa
     3661 ttgaaatatt ttttcatact tcgccgattg taaaattaag ctagacaact aaaaaaagtc
     3721 atttgtgcta cactcaaaat agttccgacg aaagaattat aaggagtgag tacaaatgac
     3781 ctataaccat cttacaccaa ctgaactcgt aatgatagaa gcttatttca atcaatcaca
     3841 acctgtttca aaagtagcca atctgttaca acgttctcga caaactattt ataaggtcta
     3901 tcgttttctt aaatctggtg gaagcgcaat gagctactat aaacaatata agaaaaacaa
     3961 acgtaactgc ggaagacatc cgattgtttt acctgatgag caacaggaat acattcaaaa
     4021 aaaggttgtt caagggtggg ctcctgatgt ccttattggt cgagcagagt ttcctattgc
     4081 ttgttcggta cgcacactgt atcgaatgtt taaagataaa acctttgaca ctcacgattt
     4141 accaatgaag ggaaaaagaa aaccaaacgg tcataaagaa aaaagaggaa aacaagggtt
     4201 tcgccggtca atccgagaca gaaagacaga ctatacccag tttgatcatg aatttggtca
     4261 cctggaaggt gacactatcg tgggtggaaa acataaaagc gcggtcatca cattagttga
     4321 acggctatct aaagtcatca ttaccttgaa acccgaaggc agacaggcta aagacattga
     4381 aaataggctg aatcaatggt ttcgttgtgt cccaaaaaac ttatttaaat ccatcacatt
     4441 tgattgtgga aaagaattct caaattggaa aaagattagt aataccaacg atatttcaat
     4501 ttattttgcg gatccaggaa caccttctca acgaggattg aatgaacatt caaatggctt
     4561 attacgtaaa gatggtttgt ataaagctat ggattttaat ggtgtatctg aaacctttat
     4621 ccaatcggtg gcatcgaaac gaaatcatat tcctagaaaa tcattacact atcggacacc
     4681 aatggaagta ttttt
//
LOCUS       DAXLPI010000150         4686 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXLPI010000150 DAXLPI010000000
VERSION     DAXLPI010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.154"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            1163..2086
                     /locus_tag="ACF3T5_002498"
     CDS             1163..2086
                     /locus_tag="ACF3T5_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFD3633023.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            2102..2641
                     /locus_tag="ACF3T5_002499"
     CDS             2102..2641
                     /locus_tag="ACF3T5_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFD3633024.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3326..3499
                     /locus_tag="ACF3T5_002500"
     CDS             3326..3499
                     /locus_tag="ACF3T5_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633025.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNGELKLNSNYYN
                     SPNEELFVKETDK"
     gene            3950..4483
                     /locus_tag="ACF3T5_002501"
     CDS             3950..4483
                     /locus_tag="ACF3T5_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633026.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            4495..>4686
                     /locus_tag="ACF3T5_002502"
     CDS             4495..>4686
                     /locus_tag="ACF3T5_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633027.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKS"
BASE COUNT         1724 a          703 c          841 g         1418 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagttttg tagaaagtac ctttaaaggt actttcacat tacgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagca aattaatgca aaatttggct
      361 agcaaaaaag ctaaaaaata tatgctaata atcgagcgaa ataaacactt tttcaagtga
      421 gcgaatccgc catgacaaag ctgaagcagt ttatttcagc tttgtcatgg cgtgtgccga
      481 cttttttaaa agtctaggca cacgagcaaa tatctttatc aagctctaat ttgcgtttta
      541 agacgtttga aacgcctaag tatataaata tataagaata ggtttaagag tgcttaaatc
      601 gaattttaga gggcatttag acaattttag ggttgttagg gcttgccctg accgcctgta
      661 aggcgtttta agaacaagat aagggattta tactaggact gtcttaaaat gtcttaaacg
      721 aacgctcaga gcttctcaaa gggttcctta tgaacggctt ttagcaaagg agccggaggc
      781 gactgcgcag tataatgtga gagcagagcg atcacacctt tttagcgaac cgtagggagc
      841 aaggtttgga attggcagtt ttgataaatt cgcgaacctg tttagagaaa aaaggagtga
      901 ttttttaggc gggaaaatta gcctcccctc tttttatatc tctttttata tctcttttta
      961 tatctctttt atgtcggctg tgtccttact ctcccaagca attcagagtt ttttcaagta
     1021 ggttttctac gcttttcaag taggttttct acgcttttca agtaggtttt ctacgctttt
     1081 caagtaggtt ttccgacatt attgagtatt tttataaagt atgttacttt tatttcatac
     1141 tttataaaag taggagaagc ttatggcaaa tgaaattgta aaatatgaaa atagattaaa
     1201 tcatattcct ctgagaaaat ttaatagtcg tgaaatgaac ttattttttt caattgcttc
     1261 acgtgtacgt gaaaaaggtg tagatgagat tattttttca tttcacgatt tgaaaaaact
     1321 aagcggttat acagaacgtg gggaacaatt tataaaagat cttagtagca cgtatgacaa
     1381 attattaagt attaatgctt ggaatgatga tggaaatact ttaactaaat ttgttgcttt
     1441 cacaaaatat agcattatac gagataaaca acttgttata atttcagtta atccagaatt
     1501 taaaggactt tttaatcaat taaagaactg gacaagattt aatctagagc aatttactaa
     1561 tttggatagc acatattcaa aaactatgtt tcgattatta aaacaatatc gaactcaagg
     1621 ctggactgaa ttttccaaag aaaaattttt tgatttacta gatattccta aaagttattg
     1681 gaatagtccg tcaaatattg acaaatttat cttaaggcca attaaagaag aactcactcc
     1741 cttatttaaa ggattaacta ttcgtaaaaa atatggtaaa ggtcgaggta aacctgttat
     1801 cggttatcgt tttacttgga aagctgaaat taaccatgct gacgattttt ccaaagggaa
     1861 acaagaagat ttacgtataa aattatttaa tattgatcac aatggagaat taacacgaga
     1921 agaaaaatgg cgcgctaaag atcgaatact aaatcttccc ttaggcactc atgaagctga
     1981 ttttaaacaa caacaacaaa ctgaagaaaa acaggcagaa aaacgagaac tttcaaacaa
     2041 actcaaacaa gatttactag gaaatttaca aaacttattc gattaagctt aaggagtatt
     2101 tatggcagat aaaacaatca aagaacttgc ggaagaatta ggagttagta aaacagcaat
     2161 taacaaaaaa gtttctgaca aaaatcgaaa actttggttt gcaaaaaacg gaaacaagtt
     2221 tgtgattaac gaaaccggtc aaaaagctat aaaatcaatg tttttagtcg ataacgcaaa
     2281 cttaaatcga aaactagttg gcgaaaaatc aaaaaccaaa aaccataaaa acaccaacca
     2341 agtttccgaa ttaaaactcc tttctttcct agaaaaacag ttaaatcgaa aagatataca
     2401 gctacaagaa aaagataatc aaataaaaca aatgcaaaaa ttactcgacc agcaacaaca
     2461 actcactcta caagcgaacc aacaaattga gaacctacaa gaacagctcc atttaactta
     2521 tacagaagaa actccaacaa ccgaatcaac tgccttttct gaaaaaaaag cagacaatac
     2581 acaaccactt attgaaaaaa agtggtggca aatttggaaa tctaatagaa ccaatctcta
     2641 aaattttcta gaagttagtt cggatagtag aggaaaaagt tgtactacct gatggggaga
     2701 tcactcaaat ttttatgaga aagaaaaaat agtttacaac tgtaaacaat caatgtttat
     2761 agttgtaaac tatcaaacta tatggtgact tattgcttaa aataggttga aatctcactc
     2821 aatttttggt ggaaaaacaa aataaattct aatcgatttt gttgaaaaag tcataaaaaa
     2881 acaaatgtga ttttaacaaa atttcaaaaa aatcgtttga aatttcgttt attttttttg
     2941 agaattttga tcatcgtagg gtcattgtag aatcattatg agatcatcgt gggatcatcg
     3001 aataaagtat gggaaaggct tgcggtgctt ggtattaagg gcattctccg cctcccaggt
     3061 tcttgaccat tttctctcaa cgtggtaaaa atcccactta taagagaaaa tggtcaagaa
     3121 cctaaacggt cggcgaagaa aacataccaa gcaccacaag caaaaaaagt tagagtaact
     3181 tccaaaaaag aaaaattttt tagaacaact tttgaaaatt taattttgat ttatatccgt
     3241 tcaatttaaa catttgataa actgaaggct agtatttaaa atgtgtatac aagtggtagc
     3301 gttaaaaaaa gtggaggtat aacctatgtt aaaaaaaact gtgttggtac tgggttctat
     3361 ttctctagga atattaggtt ttagccccat gctattatcc gtaattggaa aaaacaattc
     3421 caacggagaa cttaaattaa attcaaatta ttacaacagt cctaacgaag aattgtttgt
     3481 aaaagagacg gataaataat aaaaaagact gctctttcga gcagccagtt aaaaaataat
     3541 gagtgtttat tcttgaaaaa gcaacgcgct tatgaagcgc taccaaaaat ttatcatatt
     3601 ttatgaaaaa taacacccca gtaaaaaatg actggtaaaa attcacaaag aaacggaaag
     3661 aagcaactgc agaaaatcaa gccttaaaaa gagcttttga cttagaccaa tcgaaaatca
     3721 caaagtgagc ccctgtatcc cttggtagag aggctacttg gcatagtaca cattatgtca
     3781 acaagaattt aatataacat attttcctaa tgcattctaa ctataccaca ttatcaaaaa
     3841 tataaattat ctttactaaa taaaatattg acaagcctat accttttgtt ttatgatttt
     3901 cacaaagaaa acgttttcta tatatttttc tgtatcagga ggaaattcta tgaaaaaatt
     3961 tactttaggg gtagctttgt taactattgg ggcagttgtt ttaccgtcaa ctactatagt
     4021 ttatgcttca gaaaattccc aagtggtatc attaaacgaa aacaatgcta ctgaaaatgt
     4081 agttattgag tacactgaag atggtatggt cgtaactaaa tatgaggatg gttatagtgc
     4141 ggaagatttt gcaaattctt cagatatcat gacaagagct gcagttgcaa gatggggcaa
     4201 aaaggaatat acaaatattg ctgtaaccac tggagtagct gctaatgcta ttaatgctgc
     4261 cttttgggca ggagggaaag ctagcacttc tatgtttggt atcccaggtt gggcaattgg
     4321 tgctttgtta acaggtgcaa gttggactaa aaaaggtgct tctccaggga atgcagttgc
     4381 aaaagaatgg gacaaaaatg gtaatggctg gattggattt tattaccaaa ggggatatga
     4441 tgcagctgga agagttgtag ctactagata ttcaacgtta taagttaagg agtgatgttt
     4501 ttgatatcta aatcattaaa atcattaaaa acatttacta aaaagaatcc gcaaatagct
     4561 actattcgta tttttatatt tgctatagct gttgctataa ttgttaatat gctaagcgga
     4621 gggcaattat acaaatctgg tttattttta gtgttaatct tagttgtctt agacataata
     4681 aagtct
//
LOCUS       DAXLPI010000151         4454 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXLPI010000151 DAXLPI010000000
VERSION     DAXLPI010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.155"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(50..571)
                     /locus_tag="ACF3T5_002503"
     CDS             complement(50..571)
                     /locus_tag="ACF3T5_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFD3633028.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            770..>4454
                     /gene="nifJ"
                     /locus_tag="ACF3T5_002504"
     CDS             770..>4454
                     /gene="nifJ"
                     /locus_tag="ACF3T5_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFD3633029.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLHHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLNLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKK"
BASE COUNT         1408 a          835 c          984 g         1227 t
ORIGIN      
        1 agcgtgagac aaaagtgttc tttacttttg tcccacgctc cttatttttt tattttgttt
       61 ggatcaattc ttttaattcc tctattgaaa tagattcatc aaagatttct ttacccaaga
      121 tgacagttgg aacaaatcga atatgtgctt gctcagcttc gttgatgaca gcttggctca
      181 gctgttcgtc tttttgttct gtcagcttta gtttatctac tgcaaaatcc gctacttctt
      241 ggagagaaag atgtttccac tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg
      301 ctttttggcc atcgctgatt gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt
      361 ctttttcttt atcgaacaat ttgatcactc gctgcaattg cccgctttgg actgcttctt
      421 ctaatagctc ataagattct tcaaaccatt gtcggcaata cggacaagct aagttgatga
      481 attcgaccat ttttttagga gcattcgatg aaccataaag gattcctttt tgtgtatttg
      541 ttttcgttgc atcaatcact gatatatcca tgttaaaaca cctctttcgc ttatagttta
      601 tacatgtctg acaaaaaaag gcaacgattt agactgtttg tctaaaaatt agatgaccaa
      661 ttcaaataga tacatgtaaa atatttttat aaataaaaga aaaaaatggc atgttttacg
      721 ttataatgga acagataata agagagaata tgaacaagga tgatgaatca tgcgaaaaat
      781 gaaaacaatg gatggcaata cggcagccgc atatatttct tatgcattta ccgagattgc
      841 tgcaatatat ccaataacac ccagttcgac catggctgaa ttagtagatc aatggtcagc
      901 agcaggaaaa gaaaacgtat tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc
      961 aggagcagct ggtgtggtcc atggatcatt gaaaactggt gcactgacaa ctacatatac
     1021 cgcttcacaa gggctgcttt tgatgatacc gaatatgtac aagattgctg gggaactact
     1081 gccaagtgtt ttccacgttg ctagtcgggc agtgacgaca aatgcgttga atattttcgg
     1141 agatcacgga gacgtgatgg ctgcaagaca aacaggattt gctatgttag ctgaaagcag
     1201 tgtacaagaa gtgatggatt tagcacctgt tgcccatctt gcttctattg aaggagaagt
     1261 cccttttttg aatttctttg atggattccg tacgagtcac gagattcaaa aaatcgaagt
     1321 tttggaatat gaagaattag ctcctctgat caatcaggag aaattagctt catttcgtaa
     1381 acgagggatg aatcccaatc atccgactgt tagtggtacg aaccagaatc cagacatcca
     1441 ttttcaacaa cgggaaacga tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa
     1501 atatatgaaa gagatcaacc gattacgagg aacttcttat gacttggtga cttattacgg
     1561 agcagacgat gcggaagaag tcattgtttc tatgggatca gttgcacaga cgattgaaca
     1621 aacgattgat tacttgaatg caaacggacg gaaaacaggt tttttgaata tccatcttta
     1681 tcgtcctttt ccagtggaaa cattcgtgca aaagttgcat catacagtaa aaacaattgc
     1741 tgttcttgat cgaagtaaag aaccaggggc aggtggagaa cctctcttat tagacgttca
     1801 aagtgcgtta tatgattctg aacttcgccc ggctgttatt ggtggtcgct acggattggg
     1861 gtctaaagat gtgactcccg accaaatctc agcagttttt gatgaattga aaaaagaccc
     1921 aagcatacgc aaaaaacggt ttaccatagg gatcgtggat gatgtgactt atcaatcttt
     1981 ggaaccaaag gaaagtctga atttgacaga accgcaaaca ttccaggcca agttttgggg
     2041 atttggatct gacggaacag ttggagcaaa taaatcagct atcaagatta ttggagatca
     2101 caccgataaa tatgcgcaag gctattttta ttatgattcg aaaaaatctg gcggtctgac
     2161 tgtctctcat cttcgttttg gagatacgcc gatacgttca gcgtatcttg tcgagcatgc
     2221 ggaccttgtt gcctgtcaca caccagctta cctgcatagc tatgacttgg tcaaaggatt
     2281 gaagccaggt ggcatttttt tattgaatac tctttggagc gacgaacaat tagaaacgca
     2341 tctgccctta aaattaaaac gttacttggc tgaaaataac attagattct atacgatcaa
     2401 tgccatgaga ttggcccaag aagtcggttt gggacgccgg atcaatacgg caatggaaac
     2461 ggcttttttt aaattagctg acatcattcc ttttgatgag gtgcttccgc tgctaaaaga
     2521 agaagccctg aaaagctatg gacataaatc aatggctatc gtagaaaaaa acatacaagc
     2581 aatcgatcgg acgaccgaac tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt
     2641 ggagatgcct gaaaaaatca ataaaaggaa cactggttat atacaagaaa tacttgaacc
     2701 agtgaatgca caggaaggaa atcaactctc tgttggtaca cttgtagcaa atggaatgac
     2761 aactggcgaa atgcctttag gaacggcttc tatggaaaag cggggcgtcg ctttagaagt
     2821 acctgagtgg attagtgatc gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca
     2881 tgctgccatc cgtccatttt tagcagatga agaggaaatg gaagaagcgc cagaaggatt
     2941 tatcgtgaga gaaatgcgag gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga
     3001 agactgtaca ggttgtggtc tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt
     3061 gatgaagcct tatgaagaac agaaagaaca agctgtgaat tgggcttttg caatgacatt
     3121 aagacaaaaa gcaaatccag tcaaaggaaa gaatactgtc ttgtctacac aattcaatca
     3181 accgcttctt gaattctcgg gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt
     3241 attgacacaa atgttcggtg atcgaatgat gatcgcgaat gcgacaggct gttcttctat
     3301 ttggggcggt gcttctcctg cttctcctta tacgaccaat gaatgtggtc aagggcctgc
     3361 atggtcaaat tctttattgg aagataatgc tgaatttggt tatggtatgc ttctagcggc
     3421 acagactcgt cgcgaggctt tggcaagttc aatcaaagaa gtattgccaa aagtatcaga
     3481 agaactacga gaattgatgg aagactggtt agtacacatg tttgagagtg aaggaacgca
     3541 gcaaagagca gcaaaactgc aagctgcgat cgaaagcgaa cgatcaattt tccctgaact
     3601 ggaggatata tacaagagaa aggatctatt cgtaaaaaac agccaatgga tgattggtgg
     3661 agacggatgg gcttacgaca ttggttttgg cgggatcgat catgtacttg ccagcggagc
     3721 agacgtgaat atccttgttt tggataacga agtctattcc aatactggcg gtcaaacctc
     3781 aaaagcaaca ccagcttcgg caatcgctaa attttcggct agtggaaagt atgcttcaaa
     3841 gaaagatcta ggaatgatgg cgatgacata tggaaatgtt tatgtggcgc aaattgcttc
     3901 aggtgccaat caaatgcaaa cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc
     3961 ttctctgatt attgcctata cgccttgtat cacacatggc ttgcttggcg gaatgaaaga
     4021 atcgcttaaa gaagcaaaag aagctgtttc ttctggttat tggtcattat atcgatataa
     4081 ccctacaatg gtcgaaaaaa acaagaaccc aatgactttg gactacaaaa aacctgattt
     4141 ctctgctatg cctgatttca tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa
     4201 tccaaatctt gcaaaagctt tatttgaaaa gactgtccac gacgctaaaa cacgttttta
     4261 caactatgct gcactgacgg gagaattaga aaaaatcaag aaacgacttg aaccagagga
     4321 atctatcgat gtacccgtta agactagaaa agaaaaaacg acggaccttg aagcagaaga
     4381 aagaagagct gctagaagag ctgccagagc agaacgacgt aaaaaaatgg aacaagctac
     4441 aaaagaaaaa aagg
//
LOCUS       DAXLPI010000152         4417 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXLPI010000152 DAXLPI010000000
VERSION     DAXLPI010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.156"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            59..913
                     /locus_tag="ACF3T5_002505"
     CDS             59..913
                     /locus_tag="ACF3T5_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFD3633030.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="ACF3T5_002506"
     CDS             1010..3259
                     /locus_tag="ACF3T5_002506"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFD3633031.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4417
                     /locus_tag="ACF3T5_002507"
     CDS             3262..>4417
                     /locus_tag="ACF3T5_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3633032.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          625 c          807 g         1429 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga tatcggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actagag
//
LOCUS       DAXLPI010000153         4400 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXLPI010000153 DAXLPI010000000
VERSION     DAXLPI010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.157"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..365)
                     /locus_tag="ACF3T5_002508"
     CDS             complement(<1..365)
                     /locus_tag="ACF3T5_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633033.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTI"
     gene            complement(573..1499)
                     /gene="glsA"
                     /locus_tag="ACF3T5_002509"
     CDS             complement(573..1499)
                     /gene="glsA"
                     /locus_tag="ACF3T5_002509"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFD3633034.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1522..2949)
                     /locus_tag="ACF3T5_002510"
     CDS             complement(1522..2949)
                     /locus_tag="ACF3T5_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFD3633035.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2980..4194)
                     /locus_tag="ACF3T5_002511"
     CDS             complement(2980..4194)
                     /locus_tag="ACF3T5_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFD3633036.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1383 a          931 c          779 g         1307 t
ORIGIN      
        1 ttgattgtat atactttgct caaagaaata aattcaagat cagccagttc tgtactcgtg
       61 aatataccat taaaataata acttaaaaac tttaaaaaat aagattgacc gtacagaaac
      121 tgtgtgagct cagaaagagc aataccatgt ttgaatacga gtttatactt atgcttctct
      181 cttacttgtt cttttataca atattctaga cactcggcta gactttctag atcgtgaagg
      241 atcgttggtg tagtcaccct tagagaaata gctgtttgtt cgatgtctat ttcttggaat
      301 tcaaataatt gttcacacag atacactttt ctaaatatat ttcgctcgat gtaattttcc
      361 agcattcctg atacacgcct ccagtttttc ttatgatttt atttaatcat atctgtaaat
      421 cgtatttttg tttccttttt atttagaaaa gccctttcaa aaaccaataa aaaaagaaac
      481 tgggacaaaa gtcaaacaag ctcacaattt cagcctcatt gtaggtagct ttgagccgtt
      541 tggactttta ccccagcctc tttcaagaat ggttattcaa agatgtcagc gtctagttct
      601 ttgactacat ctttcaacaa ttggatacca gctgcgctgt taccaaatgg atctagtgcc
      661 gggctgaaga cacctatgcc gtaacgattt ggcacagctg ccatcaaccc gccgcctaca
      721 ccgctttttg caggtacacc aacatggacg gaaaactctc ctgattcatc atacaaacca
      781 gcagttgtca taattgattt gacgattgta gcactttctt cagtaatgat ttgtttgcca
      841 ttccaaggag caattccttt atttgccagc acagcagcta attttgctat gcctgtagcg
      901 gtgacattga gggagcattg tctgaaataa gcatcgagta tctccggtac gtctccttcg
      961 atcatttgat tacctttcat atagtaagca agagagcgat tgatatcacc tgttcttgat
     1021 tcagaatgat agatttcttc attcaatgtg atattaggat catcacagat ctctttcata
     1081 aatgacaaga ttcgctcaaa tttttcatct gcatctttac cggcaatcaa cgaggttgtt
     1141 gcaatcgctc ctgcattaac aaaaggattc attggatgtt tgcgatgatt gatttccata
     1201 ttcatgatag aattgaaggc aaaaccggtt ggttccgtgt tgattgtttg aaagactttt
     1261 tctattccgt tgtctttgat tgctaataac aatacgggaa cttttgacat gctttcgatg
     1321 gcaaagcgca catcggcgtc tcctccctca atcagttgtt ttgtatccaa atcataaatc
     1381 gccataccta attgtttggg atccacttca gctaatgctg gaatgtaatc cgccacttgt
     1441 cctgtttgat aatagtggaa attttttcca atcaattgtt ctaattcttt tacttccatt
     1501 tgaataacca gctttcattt tttatttttc tgcatgctgg aatttatgac ggcatgcgta
     1561 gatcgtaaat ggaataacta agacgataag ataagaaaca atcagaatcg tttcataaat
     1621 cccatgctgt ccagctgcga tactagcagg tggaacaaat gagatacaaa aagcaaaaag
     1681 tgaagtcaaa agacctaaac aagcaacgat tacttttaca acttttccgc ctggaacctg
     1741 ataggctctt tcaagactgt catctttaaa aaccagcttc aaatagcccg cgaaaaacat
     1801 aaagtacaac atcaggtaga ttacaacagt caatgacatc gctgcaagaa atgaaagatt
     1861 attccccccg ccgcttaatg taaagacggc tgcccagatt gttgcaacga tcccatttgc
     1921 gatcatcaaa tgagcaggaa caccgtgtgt gttttctttt gcaaaatatt caggaagtaa
     1981 tccttcttct gctgctacgt gaagtgcttt tgaaggagag gttacccaag aagcaatttc
     2041 tccgatgaca cctaaacaaa caagaatcgc aagaattttc gcaatccatg caagatttgg
     2101 atcaatgtgt cttaatagga attctaacga ttgaatcacc cctgtattaa gagacaaacc
     2161 atgttgaggt actgtagtag cgacggtcaa tccgccaaaa gtatctaaaa tgattgccag
     2221 aataaccaat acaaacattg ctaatggata atccttttta gggttttcca tttcatttgc
     2281 atgactggca gatgtttcta cgcccatata tgctaaaata aaggaaacaa aaacaaccag
     2341 cgtaggcaat ttcgaaaaat caggaatcaa tgttttcata gacagtatcg tttgtagtgg
     2401 agcgcctgaa gcgacataat ggatccctaa gccaaacaag actaatgctg gcaacaagat
     2461 accagctaca aaaccgatcc gagcaatcaa tgaagtgtat ttggttcctt ttaactgtga
     2521 gagtaaaata gcccagaaaa taatcaatag gattgccaat ttcagatatt tattgttttg
     2581 tatagcacca atacccgttg cataggataa tgcaccagtt aagaaataca gcatggtcaa
     2641 gtagccaact gttatctcaa accattggaa aaagattgcg gcaaaaccga atcgttttcc
     2701 caatgtgcga ctgacccaag tatagacacc gcctttttcc catcctttca ccgttgccat
     2761 ctctgctgcg cacaatgcca cagggataaa ccatagcaaa ccgcctaata acaagaaaaa
     2821 tactaagctg aagcctgagg tcgcgaatgt gggatattca tataatgaaa cgaccattga
     2881 gatggtcata gccaaaaagc caaagaacgt taattttttt gcaggaattt tcgttttgct
     2941 ttgttgcatg aatcttcctc ttttctattt gaatttgttt caattgagtt tgttgccaaa
     3001 gaagaaaaag tccaatcctt cttcttgttc ttcttttgta gtagtgatcg ttgtaaagtg
     3061 catcgccgat tttaaaatca ctgttgtcag cacataggca tacacggcag taatgatgac
     3121 accaatcact tgaatcccta agaagtacca gctgcttgga ttcgttagta aatggctgtt
     3181 agatgcaaag atccctatca gaatcgtacc tgtgaatccg cccatgccat gaacacccca
     3241 cacatcaagt gcatcatccc attgtttctt tttacgaaaa tcaacagcag catgacagac
     3301 gatacccgca atgatcccaa tcagcatggc actttttgct tctacataac cggcacaagg
     3361 cgtaatggct gccaatccag caacggatcc tgttagtacg tcgacaaaat caaagccatt
     3421 tccttttact tttgcaatga tcagccaagt aatcatcgca aatgcaagag cgatgaatgt
     3481 tgaaccaaat gcattgactg cttgttcatc tgctcgaagt gcaccaccgg aattaaaacc
     3541 gaaccagcca aaccaaagca gtcctgtacc gatagcagct gccataagac tgctatgctg
     3601 acttctttct cctaaaggaa tctttctttg accaagtgtg aggatacatg ctaaagagcc
     3661 gaatccagct gtcgtgtgga tgactgctcc accagcgaaa tctctaaatc ccatctgatc
     3721 caaaaatccg ccaccccata tccagtggca aacagggaaa taaatcagca gattccaaaa
     3781 aataacaagt ataatatagc cttttaaatt caatctccct gcaaaagctc ctgtcatcaa
     3841 aggaacggta atcacgataa acatcagttg gtaaagaaag aacattaaaa atggaatcgt
     3901 tgcaccatga aacatgttgg ggaagcttgt aacatgtctc atcaaaaaga aatctgccgg
     3961 gtttccaata acaccgccga tatcccgtcc aaaggccaat ccgaatccac caaataccca
     4021 cataatcgtt gttatcccaa cggagagaaa agattgagac atgatcgtta gtatattatt
     4081 cttccgtgct aatccgccgt aaaaaaaagc tagaccagga gtcatcaggc aaaccattgc
     4141 tgtacataaa atcatgaatg ctgtatctcc tgtattgata ggtaatgtat tcattttttt
     4201 cgcctccttt ctacgattac gagtataaaa gaaggtgtag ttgttttttt cttctgtttt
     4261 tttgaattaa tacttgcatt tataaaagac agacaattaa taaaagcttg atgtgttgtg
     4321 ctctttttcg tttttcacac aaataaagac aaaaaataaa cggtcttctt tttatagaaa
     4381 accgtttatt ttcgtctttt
//
LOCUS       DAXLPI010000154         4372 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXLPI010000154 DAXLPI010000000
VERSION     DAXLPI010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.158"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(83..1051)
                     /locus_tag="ACF3T5_002512"
     CDS             complement(83..1051)
                     /locus_tag="ACF3T5_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFD3633037.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1496..2902)
                     /locus_tag="ACF3T5_002513"
     CDS             complement(1496..2902)
                     /locus_tag="ACF3T5_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HFD3633038.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSENEIEW"
     gene            complement(3124..3486)
                     /locus_tag="ACF3T5_002514"
     CDS             complement(3124..3486)
                     /locus_tag="ACF3T5_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633039.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3490..3798)
                     /locus_tag="ACF3T5_002515"
     CDS             complement(3490..3798)
                     /locus_tag="ACF3T5_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633040.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3807..4226)
                     /locus_tag="ACF3T5_002516"
     CDS             complement(3807..4226)
                     /locus_tag="ACF3T5_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633041.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
BASE COUNT         1241 a          979 c          604 g         1548 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt aatttttccc
     1021 actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag aaaaaagaga
     1081 tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca tcaatctcta
     1141 gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac tagtgtggtt
     1201 agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt tgctcttttt
     1261 ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc aaataccaaa
     1321 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taagttggcc
     1381 aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg gtttagccgt
     1441 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtggctacc
     1501 actcaatctc attctcagaa cgctctaatt tatgctgtga gacgttttga cgataaaata
     1561 aggttttctc ctgctctaat acttctaatt cctcagaaag ccccatatcc ttttctgcaa
     1621 gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt aatcgctcta
     1681 aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact tgtaaatctg
     1741 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1801 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1861 tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1921 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct ttttggaaaa
     1981 tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca atcttcgctc
     2041 tctcagaagc catcagagcc ttatcacgct cgttttcttc tctttgccta taaatatacc
     2101 tcaactcatc agaaacgctc tcacgctctt ttacagccgt ttggcgcaat tcagaaattt
     2161 cactcatgat ttctttgtat tcgtgaatat ctttaatttt gttggtaccg acaatatccc
     2221 ttttccaacc gtaatcattc attaatcgtt ctatagagtc aacttcttga tttctaaaat
     2281 tattgaaaag cactcgacca ttatctttat cagcgtgtat cccttgttga accagtgctt
     2341 tattaaagct cggctgcttt tgaacacccc gtttataacc ttcagcaacc ggcacaatat
     2401 tcaagtgcag gtgcggactt atttcgtcat tatgaataac agcattataa acagccaaat
     2461 tagggtttct ctcttgaaac tccttgtaat actgaaccaa aattttattg gcaatcactc
     2521 gattttgttc agcttgttgt ttcgatatgc cagtagaaga accatctgct cgatttgtct
     2581 ggaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc aactttttat
     2641 ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt tgtttcgcat
     2701 tatatttttc taccacttcg ccaaacaatt cctcatattt atctgctaga ttcgtctgct
     2761 ctaaataaat attttcatcc gatttcgtga agtcaatgtg cttatgccaa tcattggttt
     2821 tctggcgatc agttaactca cgattattat gagaaatact tgtattcttc gttccttttt
     2881 taaatgaaat actaaacccc aagttaaaac cctccttcgc gctgtcgcta ataaaaagat
     2941 tgtaaacctt tctgttactc tttgtcaaag agtaacattt tcctaattgt gacatggatg
     3001 tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag
     3061 ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac gcaaaataat
     3121 cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac tcatctgact
     3181 tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat ctcgttcgct
     3241 tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt gttgctgatc
     3301 gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc caaaacgctt
     3361 gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt tctgttggcg
     3421 aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg ctaaagtcat
     3481 tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt ttaactgcat
     3541 aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg tttgcgctaa
     3601 atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag cattgataac
     3661 cgagttgaga acatccataa ttcgttcaag catttcacta taattatctt catccggcaa
     3721 attcaaataa tcagctaacc gttcatacat caattcaatt ctatcgactg tttcttctct
     3781 caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt tcgatttttt
     3841 aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa agtcaatttt
     3901 tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc cgctaattgt
     3961 tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc attgaccggt
     4021 aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac accaatcaat
     4081 tccttgtcta acatgataac cccattatct ttataccgag caaggtcata acctgcattg
     4141 accagttgat tgcgcactgt atttttagat atacccaact catcagcaag gttttttatg
     4201 gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta ccccataaac
     4261 atatttaaca gcatgcttag ggataatgat tggggaatca attttattat ttttcaaaat
     4321 caactcatac tgcgttacat caagtaactt accggacatt tttttgctat cc
//
LOCUS       DAXLPI010000155         4322 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXLPI010000155 DAXLPI010000000
VERSION     DAXLPI010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.159"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            287..694
                     /locus_tag="ACF3T5_002517"
     CDS             287..694
                     /locus_tag="ACF3T5_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633042.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="ACF3T5_002518"
     CDS             708..1109
                     /locus_tag="ACF3T5_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633043.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="ACF3T5_002519"
     CDS             1111..1482
                     /locus_tag="ACF3T5_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633044.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1820)
                     /locus_tag="ACF3T5_002520"
     CDS             complement(1518..1820)
                     /locus_tag="ACF3T5_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFD3633045.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2078..2191)
                     /locus_tag="ACF3T5_002521"
                     /pseudo
     CDS             complement(2078..2191)
                     /locus_tag="ACF3T5_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2574..3807)
                     /locus_tag="ACF3T5_002522"
                     /pseudo
     CDS             complement(2574..3807)
                     /locus_tag="ACF3T5_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            4064..>4322
                     /locus_tag="ACF3T5_002523"
     CDS             4064..>4322
                     /locus_tag="ACF3T5_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3633046.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFN"
BASE COUNT         1449 a          715 c          767 g         1391 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaattc
     1741 catactttat aaaaatcttt gataaatcta ttcgaagggt caaatttaaa ccataattgt
     1801 gtttttcctt ccatcagcat aaaagttcac ctcaaaaaga gtatacgaac gaatgttctt
     1861 tttgtgaagg cggaatttaa tatactgaat aatagaaaac aagtacttgt gccaaattat
     1921 gtgccaaaaa aattcgaatt caacaaaatg taaacaaata gaaaaccttc tatattaaga
     1981 tttcctgcaa taacaaacac aacagaagac gtgtaatagt tagtcaggaa cgtacaaata
     2041 acccctgtat cctttgcggt acagggggtt attttgtata agtagacatt gtagtttcta
     2101 aactgctatg tcctaaacgt ttcgatacgc taagtatatt tactccttga taaagtaaaa
     2161 tagatgcatg cgtatgtctt agtccctcaa taaacagtcg aagagaaaat aggcaaaaat
     2221 tgattactaa agaaaaactt actaggtcga cggtgtagtt gattttcata cttcaacaga
     2281 ttcgaattaa acgatttcga ttggcttata ccagtcctat ccgacgaaag atatatcgtt
     2341 cttaactcta gcggtataac ttgaagcttt acgtaaagcc aatgggaatg aactggagat
     2401 gactttttat tatttttagt aatagctgat gggttaatac ttacaacttt ccttttcaaa
     2461 gtaccatata aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt
     2521 agatccttta ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc
     2581 caatcgccat ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc
     2641 atcttgtcag atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct
     2701 gctaattctt catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct
     2761 agtgctaagt gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat
     2821 tgagaaatag gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca
     2881 tctgtatcaa gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaatgact
     2941 tttccatctt taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc
     3001 tttgtacttg aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc
     3061 gtcggcatat ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa
     3121 agatggtttt taggcaatcc gataatgata tctgtaccgg gagcagtata gtgcagggca
     3181 gaaaattgtt ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc
     3241 catgctaaga cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat
     3301 gcctcaactt gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca
     3361 gccgcagcca cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt
     3421 agtgctttgc cagccgctga ttgataagac ggctactcga tggctatcta caccagcaaa
     3481 tgcatcggga tcggcagaaa caacactgat cctgcttgct cctttttcaa gccattcatc
     3541 cgcttgatcg attttagatt gtggtacatt ctcaatacga tccgttgcag catgtaaaag
     3601 aaattctctt tggatctggt cgtcagtcca ttgaacgata acctcagcgg ctcctagttt
     3661 ataagcttct tgtgtgatca atcgagcaag gggtgcctgt tccacgctga tctgtaaaac
     3721 aactgtgtgt cctttttctg tggctacgcc agtttctgca attaagcgtg catatttttt
     3781 tagaagttca ttaaaatcag ataacataat aaaaattcct cctcaccatt ttttctggta
     3841 actattagca tgataccatt taattatgat tcaaacaatg aggcctttaa ataaaaaaaa
     3901 gaggttattt ttttaaggtt aagtttcatg atatatgtta ctacttgcta tttcagcttg
     3961 tgaagaaaat caaaaactaa ttttgcatat caaaaaaaac atgttacaat gaaattaatc
     4021 aatggtggaa gcgttataaa aaaattaagg agattaaaaa aatatggcaa gaaaaaagac
     4081 gattactaga gaacagattt tggcagctgc ttatgaagta gctgccaccg atggatttac
     4141 acgatttact gcgagaaaca tcgcgaacaa aatgaattgt tccacgcaac cgatttatct
     4201 tgagtttaaa aatatggatg acttgagaca agctcttttt gagcagatct atgatcattt
     4261 acaatatgaa gtgttcactg ttgagcatac agggaatacg attatcgatc ttgcttttaa
     4321 tt
//
LOCUS       DAXLPI010000156         4282 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXLPI010000156 DAXLPI010000000
VERSION     DAXLPI010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.160"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(37..1257)
                     /locus_tag="ACF3T5_002524"
     CDS             complement(37..1257)
                     /locus_tag="ACF3T5_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFD3633047.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="ACF3T5_002525"
     CDS             complement(1277..1888)
                     /locus_tag="ACF3T5_002525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFD3633048.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="ACF3T5_002526"
     CDS             complement(1931..2863)
                     /locus_tag="ACF3T5_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFD3633049.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="ACF3T5_002527"
     CDS             complement(3284..4144)
                     /locus_tag="ACF3T5_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD3633050.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DAXLPI010000157         4258 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXLPI010000157 DAXLPI010000000
VERSION     DAXLPI010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.161"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            223..549
                     /locus_tag="ACF3T5_002528"
     CDS             223..549
                     /locus_tag="ACF3T5_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFD3633051.1"
                     /translation="MKKNSKEFRNEYDRFVLKFLIDNYYISRIDLSKAIGLAPSYVRE
                     FYNGSRSFGNEALEKLESTIFNLYKPLLENHSFELNQVQEMIESIDSEEELELFRLKG
                     ANVLDI"
     gene            934..2427
                     /locus_tag="ACF3T5_002529"
     CDS             934..2427
                     /locus_tag="ACF3T5_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFD3633052.1"
                     /translation="MTASLDVVYSTILQNGIRKFKYKNSRLKPVSFSEQTGKGAIFAY
                     RSKEHMVEGIGLVITSEEGIIENNNAFSHWTPNVFRYGTYTDEGRMFTKGHSEDNLRQ
                     INTFFVDFDTLDPNFDYGEILLASHELGFMPTMVLRTPHGYQAFYVLDQPAFVTKKSN
                     FKVINVAKMISNNLRKTLAKDFPVDVNANHFGITRMPNEDNTLFFEPQYRYSFADWIN
                     WSMRVDADKAVTYRNVFELPSNQDFRQVDEPWFRLLMRSEKIKGEKGRYGRNNVIFTL
                     SLAYFSSGYEIEECELAMLEFNDRLAYPLTDRELTRIIRSAYSGKYQAAHREKIILLC
                     QEWIDENLSEEQLFNKKKGWWKFKKERKVRKYSHSHEWAQDLLAYLNQQSYTYKPYIV
                     STKKELREELKIPERSLDKVLKQLKNENKIYYRVRSGRNGGITLASVHGLMRSIIRVK
                     KEVQEAYFSAISEVFGLARTFIYSTLEQLIEAPKKAEQMELFEVDTG"
     gene            2585..2869
                     /locus_tag="ACF3T5_002530"
     CDS             2585..2869
                     /locus_tag="ACF3T5_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFD3633053.1"
                     /translation="MGVNTFVYPYPVSVFIIQRLGMTVEEFCELHGYRQSTVATWAYR
                     NRPVKVLPCDFLYCLSLASGLSMDKVYQDLLLLEEKFLQSLDPKKEKKSI"
     gene            2905..3030
                     /locus_tag="ACF3T5_002531"
     CDS             2905..3030
                     /locus_tag="ACF3T5_002531"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633054.1"
                     /translation="MFRVEIVEKKTIEKLEKAVNNILISKGDKVQTIEYAAVKKI"
     gene            complement(3257..3937)
                     /locus_tag="ACF3T5_002532"
     CDS             complement(3257..3937)
                     /locus_tag="ACF3T5_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFD3633055.1"
                     /translation="MNHFKGKQFQKDVIIVAVGYYLRYNLSYRDVQEILYNRGINVSH
                     TTVYRWVQEYGKLLYNIWKRKNKQSIYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPRVLVTDKAPSIASAFKKLQAHGFYQRTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYRSLRTASTTIKGMEAIRGLYKKNRKEGTLFGFSVCTEM
                     KILLGIPA"
     gene            4123..>4258
                     /locus_tag="ACF3T5_002533"
     CDS             4123..>4258
                     /locus_tag="ACF3T5_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
                     /protein_id="HFD3633056.1"
                     /translation="MSTVILAEKPSQALAYASALKQSTKKDGYFEIKDPLFTDETFIT
                     F"
BASE COUNT         1378 a          681 c          876 g         1323 t
ORIGIN      
        1 taaatcaaaa aataaaacag cagattttat attatgttac tattgataat agtaacataa
       61 tagtaagata atatattata ttactattat gttacctttt aaacactgat agatagggaa
      121 tttgaagaat tatattaaaa aaaggattgt atataaattt ttttgatggt aatattataa
      181 acgaggatgt tgctataatt aacaacgaag aggtaagatt tcatgaaaaa aaactctaaa
      241 gagtttagaa acgaatatga cagattcgtt cttaagtttt tgattgataa ttattatata
      301 agccgaattg atctatctaa agctattgga ttagctccta gttatgtgcg agaattttat
      361 aatggatctc gtagctttgg aaatgaggca ttagaaaaac tagaatcaac aatttttaac
      421 ttatataaac ctttgctaga gaatcattca tttgaattaa atcaagtaca ggaaatgata
      481 gaatcaattg attctgaaga ggaacttgag ttgttcagac ttaagggagc taacgtatta
      541 gacatttaga taatttctac ttgatttggt aaattatatt tacaactttc aacagattcg
      601 gtaagataaa aaaaatatat aaaaaagaga cggataaaca agtgaattgg acctcactca
      661 tttatctgaa tcacataaag caactatgtg ttctcgctcg tctcaaggaa attatgtaag
      721 tggtagccaa ccacttatgt gatttgaata aatctgttcg gactaccaat ccgaacctgt
      781 aggtttattt taccattttt agacgggcaa ctcaatagat aaacgcttat ttgtcatgct
      841 ttctttagat tgttttgata tttctagcga ttgagagaac acagatcgaa cgattatttg
      901 tgttctcttt ttgtttagaa aggataagta ggcatgacag cttctttaga cgtcgtttat
      961 tcaacgatct tacaaaatgg tatccgtaaa tttaaatata aaaatagccg ccttaaaccg
     1021 gtctctttta gcgaacagac ggggaaaggg gctatttttg cgtaccgttc aaaagagcat
     1081 atggttgagg gaataggctt agtgatcacg tctgaagagg gcattatcga gaataacaat
     1141 gcgtttagtc actggacccc aaacgtcttc cgctatggca cgtatactga tgaaggcaga
     1201 atgtttacaa aagggcattc tgaggacaat ttacggcaaa tcaacacttt ctttgttgat
     1261 tttgacacct tagacccaaa ttttgattat ggagagattt tgttggcaag ccatgagcta
     1321 ggatttatgc caacaatggt tttgcggact ccccatggtt accaagcgtt ttatgtactg
     1381 gatcagccag cttttgtgac aaagaaatcc aattttaaag tgatcaatgt ggccaaaatg
     1441 atttctaaca acttgcgaaa gacattagcc aaagattttc ctgtagacgt caatgccaat
     1501 cattttggaa tcactcgtat gccaaatgaa gacaatacct tattctttga gccccaatat
     1561 cgttatagct ttgcggactg gatcaactgg tcaatgcgtg tcgatgcgga taaagcagta
     1621 acctacagaa acgtttttga attaccgtca aatcaggatt ttcgccaagt ggacgaaccc
     1681 tggttccgtc tattgatgcg tagtgaaaag attaagggag agaagggaag atatggtcga
     1741 aataacgtca tatttaccct gtctttagca tacttttctt ccggatacga aatcgaagag
     1801 tgcgagttgg ccatgttaga gttcaacgat cgattagcct atcctcttac tgatcgggaa
     1861 ctgacacgaa ttatccgatc ggcttattca ggaaagtacc aggcagcgca cagagaaaaa
     1921 attattctat tatgccagga atggatcgat gaaaatttat ctgaagaaca gctgtttaat
     1981 aagaaaaaag gctggtggaa attcaaaaaa gagcgtaaag tgcgcaagta ttcgcacagc
     2041 catgagtggg cgcaagatct cttagcttat ttgaaccaac aatcatacac ttataaacct
     2101 tatatcgttt caaccaagaa agagttaaga gaagagctta aaattcctga acgctccttg
     2161 gataaagtat tgaaacagct gaaaaatgaa aataagatct actatcgtgt tcgttccggt
     2221 cgcaatgggg ggataacttt ggcgtcagtg catggcttga tgcgatcgat cattcgagtg
     2281 aagaaggaag tccaagaggc gtattttagt gctatttcag aggtttttgg attggcacgt
     2341 acatttatct actcaacgct ggaacaactc atagaggcac ctaaaaaggc tgaacaaatg
     2401 gaattgtttg aagtggatac cggataaaaa taaaacccgc aaattgcctt ttacattata
     2461 tctttgatac gctctccgag tcagaggttt tacaggtctt agggaattcc cttgattgca
     2521 atgtttggat aagtgaggta ctattatcgc atatgtgatt atatttctta gggggttacc
     2581 gtccatggga gtgaatactt ttgtttatcc ttatcccgtc agtgttttca ttatccagcg
     2641 tttaggtatg actgtggagg aattttgtga gttacatggt tataggcaaa gtaccgtagc
     2701 aacttgggct tatcggaata ggcctgtaaa agtcttaccc tgtgattttc tgtattgttt
     2761 gagcttggct tctggtttgt caatggataa ggtttatcag gatttattac tgttggagga
     2821 gaaattttta caaagtttgg atcctaaaaa ggagaaaaaa tcgatttaaa gtttagtaag
     2881 tttgaaagga agagggggat aataatgttt cgtgtagaaa ttgttgaaaa gaaaacgatt
     2941 gaaaaattgg aaaaagcagt gaataatatt cttattagta aaggggataa agtacagact
     3001 atagaatatg ctgcagtaaa aaagatttaa acgtctatta ttctgctatg attgtatata
     3061 aggcttgagg atgttttgag ttgttaggaa tctaagggaa gtaaaaatat ttctatagga
     3121 gaacagaaaa gatggataat gatcattgtg agaatgtaaa caagttagaa agtgcgggta
     3181 aaacttatta tattattggt gcggttctgt tgcaaagttc caaaatgaac aatactagtc
     3241 taacactagc tatagtttat gcaggtattc ctaataatat cttcatttcc gtgcagactg
     3301 aaaacccgaa gagagtgcct tcttttcggt tcttcttata taatcctcga atggcttcca
     3361 tgcctttaat cgtggttgag gctgttcgta agcttcgata aaatttgttt cgtctcttga
     3421 tgggacgatg atcttgttca atgaggttat ttagatattt caccgtgcga tgttctgttc
     3481 gctggtaaaa cccgtgtgcc tgtaattttt tgaaggcact ggcaatagag ggagctttat
     3541 cggtcaccag aacccttggt tcatcgaact gtttaactaa tctcttgaga aaggcgtaag
     3601 ctgcttgtgt atctcgcttt ttccgtaacc atatatccag agtgagacca tctgcatcaa
     3661 tggcgcgata aagataatgc cattttcctt tgattttaat ataggtctcg tccattttcc
     3721 acgaataaat ggactgtttg tttttcctct tccaaatgtt gtaaagtagt ttcccatatt
     3781 cctgtaccca acgatagaca gtggtgtgag acacgttaat accacggttg tacagaattt
     3841 cttggacgtc acgataactc aggttgtacc gaagatagta gcctacagcg acaataatca
     3901 catctttctg aaattgtttg cctttaaaat ggttcatctt cgttactcct cctagttcct
     3961 attaaactca gtttacccta taatggatct gaggggaaac tttgcaacag aaccagttga
     4021 agaacaaact ggaatcaatc gccgaacgtt tagaaggtat cgagcaagat ataacgtgac
     4081 agtagatcaa agaaaaaaca atgaaaagag ggatagttaa tgatgagtac ggttatttta
     4141 gctgaaaaac caagccaggc attagcctac gcaagtgctt taaaacaaag caccaaaaaa
     4201 gacggttatt ttgagatcaa agacccacta tttacagatg aaacgtttat cacctttg
//
LOCUS       DAXLPI010000158         4234 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXLPI010000158 DAXLPI010000000
VERSION     DAXLPI010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.162"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(220..699)
                     /locus_tag="ACF3T5_002534"
     CDS             complement(220..699)
                     /locus_tag="ACF3T5_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFD3633057.1"
                     /translation="MIKFLRKKKQVELFSPCSGKVIPISEVNDPVFSQKLLGEGFAVI
                     PEEQTIYAPLAGQVTTIFPTKHAISIKSNSGIDYLIHIGIDTVELEGKPFSILVDEKE
                     YITPENPLVKVDFKQLELAKKDSSVIICFTESSQIDNLDLYTGRVLHGVLCGYLNLS"
     gene            complement(711..2213)
                     /locus_tag="ACF3T5_002535"
     CDS             complement(711..2213)
                     /locus_tag="ACF3T5_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFD3633058.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPIL
                     AQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVSYLVYTATI
                     SGFLELFSVKDATLDTGVVGSIAIGGIVAFLHNRYRKIELPQFLGFFGGSRFIPIISS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEHIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTESVAGITVSGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGLAWAIIYFIVFTFFL
                     KKFKVAIPGMIEEKEVVSEINLNKNTSLHEESLMIIKALGCEENIESVEACATRLRVA
                     VKNGQNVDKQAIKQLGATAVFEVKGGIQAVFGGKADLLSQEINQILKRDN"
     gene            complement(2206..2913)
                     /locus_tag="ACF3T5_002536"
     CDS             complement(2206..2913)
                     /locus_tag="ACF3T5_002536"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFD3633059.1"
                     /translation="MKKQIFLDAISGGLIVSCQALPGEPFYKEEGGLMSYFALAAERA
                     GAVGIRANSVRDILEIKEKVSLPMIGIIKRDYPPEEPFITATMREVDELVATGVEVIA
                     LDCTLRKRHDNLSINTFITQIKEKYPEQLLMADISTFEEGKNAYEAGVDFVGTTLCGY
                     TNESRKQEGPAIDLMKQLVQAEICVIAEGKIHTPEQAKKIRELGVSGIVVGGAITRPQ
                     EIAERFVNALKEEEKNV"
     gene            3076..3894
                     /locus_tag="ACF3T5_002537"
     CDS             3076..3894
                     /locus_tag="ACF3T5_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFD3633060.1"
                     /translation="MNYIYLIKEHYPLLTKSEKKVADLILDSGKAIIYSTMSDIKEKT
                     TVGDATIIRFCQKLGFSGFSDLKIEIAKEDFSKKKEYPSSEKYYDIIATHLTEALYST
                     AHLLDEKKLDKAIQLISQAKNLYIFGVGSSGNTSLDLESMFLRVGVQAKAVLDPHFQA
                     QVASLMEEDDVIIAFSLSGKTKDTFDSVEIAKKNGAKILAITNYLHSPIGQTADVVLQ
                     TAIEEFLNGGSLAGKISQLYLCDLLVQGYEQKNNINAVELRERVLRSILDKSLD"
BASE COUNT         1504 a          791 c          634 g         1305 t
ORIGIN      
        1 tacacgtggg cgagctgact aaaatcatat aatcaaaaac ttaataaaac gaggttcact
       61 ccaatcatat ttcatttttc aaaactatca cattaatctc ttgtcaatca aaattattat
      121 acattgagaa agttacaact tatatactac ttctttaaac tctaaaactt caccaatgag
      181 tttctaataa ttagatctta ttaatctaac tattaatcat tatgataaat ttaaataccc
      241 acaaagaaca ccatgtaaaa ctcttcctgt atataaatcg agattatcaa tttgactaga
      301 ttcagtaaaa caaatgatta ctgatgagtc ttttttagcc aattcaagct gtttaaaatc
      361 aaccttaact agtggatttt caggtgttat atattctttt tcatcaacaa gaattgaaaa
      421 aggttttcct tctaattcta ctgtatctat cccaatatga atcaaataat caattcctga
      481 attactcttt atactaatag catgttttgt tggaaagata gttgtgacct gaccagctaa
      541 aggagcataa attgtctgct cttctggaat aacagcaaaa ccttccccta ataatttttg
      601 tgaaaaaact ggatcattta cttctgatat aggaataact tttcccgaac atggagaaaa
      661 tagttccact tgttttttct ttcttaaaaa cttaatcata taaattcctc ctaattatct
      721 cttttcaaaa tttgattgat ttcttgacta agtaaatcag cttttcctcc aaaaactgct
      781 tgaatcccac cttttacctc aaaaactgca gtagcaccta attgttttat agcttgtttg
      841 tcaacatttt gtccattctt cacagcaaca cgcaagcgag ttgcacaggc ttcaacagat
      901 tcaatattct cttcacagcc aagtgctttt ataatcatta aagattcttc atgcaaagaa
      961 gtatttttat tcaagttaat ttcagagact acctcttttt cttctatcat accagggatc
     1021 gctactttga atttttttaa aaagaaagta aaaacgataa agtaaataat agcccaagca
     1081 agtccaaaag gaatcaattt aagccagtta gttttgtcat ttccttgaag tacaccaaat
     1141 aatagaaaat caattagtcc acctgaaaaa gaatttccta ctcgaatatt caggatatcc
     1201 gcaaaataaa aagataggcc gtctaaaaat gcatggatga cataaagcca tggagccaca
     1261 aacagaaaca tatattctac aggctcagtg attcctgtta aaaacgaagt taatgcaccg
     1321 ctgaaataaa gaccgccatt tttcttacgg ttttttttag gaattgcacg atacatcgca
     1381 taacaagcag caggaaggcc aaacatcatc gtggcaaatc ttccagcaaa aaaacgagtc
     1441 ccataagtaa ataagcctgt gtgattcgga tcagctaatt gagcaaagaa aatattttgt
     1501 gccccagaaa ctgtaattcc cgctacactc tcagttcccc ctaacgaagt gtaccagaaa
     1561 agtggataaa ttgtatgatg aagaccaaca gcaccagtta aacgaagtaa aaatccataa
     1621 agaaatgttc ctagactccc catttgagca atatgctctc cagcaacaac taatccattc
     1681 tgaatcggtg gccaaattag ataaaagaag gaaccaataa taattgcagc caaactagaa
     1741 ataattggaa taaaacgtga accaccaaag aatcctaaaa actgcggtaa ttctattttt
     1801 ctatagcgat tgtgtaaaaa tgcgacaatt ccgccaatgg caattgaacc tacaactcca
     1861 gtatcaagag ttgcatcttt gacagaaaat aattcaagaa aaccactaat tgttgctgta
     1921 taaactaagt aagaaacacc accagctaaa ccagcagttc ctttgtcacc atttgcaagc
     1981 cccactgcga taccaatcgc aaatatcaat gctaaattag caaaaacggc gttaccagca
     2041 taactcatga tactcaaaat cgtatgaagc cacggttgag ctaatatggg ataagcattt
     2101 atggcgcttt catttgtcag tgcaccgcca agacctaaaa gtaaaccagc aacaggtaaa
     2161 atagcgatcg gcaacataaa tgcccgacct agttgtgaaa attttttaaa catttttctc
     2221 ttcctccttc aatgcattga cgaaacgttc tgcaatttct tgtggacgag tgatcgcgcc
     2281 accaactacg attccagaaa cacctaattc cctaattttt tttgcctgct ctggggtatg
     2341 gatttttcct tctgcaataa cacaaatttc tgcttgaacg agctgtttca tcaagtcaat
     2401 cgctggccct tcttgctttc tactttcatt agtatatccg cataacgtgg ttccgacaaa
     2461 atcaacgcca gcttcatagg catttttacc ttcttcaaag gtagagatgt ctgccattaa
     2521 taattgttca ggatactttt ctttgatctg agtaatgaaa gtgttgatag acaaattatc
     2581 atggcgtttg cgcagcgtac aatctagcgc aataacttct acaccagtag caactaattc
     2641 atcgacctca cgcatcgttg cggtaataaa tggttcttca ggaggataat ctcgcttaat
     2701 tatcccaatc ataggtagag aaactttttc tttaatctct aaaatatctc gaacagaatt
     2761 tgctcggata ccaactgcac cagctctttc agcagctaga gcaaaatagg acataagacc
     2821 tccttcttct ttataaaatg gttcaccagg taatgcctga caagaaacaa tcagtccacc
     2881 tgaaattgca tctaaaaata tttgtttctt cattctccta aacactccac tttaaattaa
     2941 ttttcggata ataactccaa ataaaatata gcacgcgctt tcattgatgt aaagtataaa
     3001 aatggataaa tttggagatt tttttatctt tttggagtat aatggagtaa aattttttac
     3061 tgtaaaggag ttcatatgaa ttacatctat ttaattaaag agcattatcc tttattgact
     3121 aaatctgaaa aaaaagtagc tgatttaatc ttagattctg ggaaagcaat tatttatagt
     3181 acaatgagcg atatcaaaga aaaaaccaca gttggggatg caacgattat tcgtttttgc
     3241 caaaaattag gattttctgg tttctctgat ttgaagatcg aaattgcaaa agaagatttc
     3301 agtaaaaaaa aagaatatcc gtcaagcgag aaatattacg atataattgc tactcatcta
     3361 actgaggcgc tctattctac agcacattta ctagatgaaa aaaaactaga taaggcaatc
     3421 caactcatca gtcaagcaaa aaatctttat atatttgggg tgggttctag tggaaataca
     3481 agccttgatt tagaaagtat gttcttacga gtaggagttc aagcaaaagc tgttctggat
     3541 cctcattttc aagctcaagt agcttctttg atggaagaag acgatgtgat tattgctttt
     3601 tcattatccg gaaaaacaaa agataccttt gattctgtag aaattgcaaa aaaaaatggt
     3661 gcaaagatct tagcaattac caattacctt cattctccaa taggacagac agcagatgtt
     3721 gttttacaaa cagcaatcga agaattttta aatggtggtt ctttggcagg gaaaatctct
     3781 caattgtacc tttgtgattt acttgtacaa ggttatgaac agaaaaacaa tattaatgca
     3841 gtggaattac gtgaaagagt attacgctct atcttagata aaagcttaga ttaaatagag
     3901 tattaattga agcaatttat atttaggtga aatatcaatt aattagtggg actcacaatt
     3961 tgcacaaata actttcatct tacctttaga tgaagtgtta gaaagaaaag tatcaaccat
     4021 caatactttg aaaaaattct ttttatagga atatgatgaa cagctttcaa tatacaataa
     4081 tcattatcaa agataactga caacttttag caataaaata caaaacactt ttttagtcag
     4141 tatctaaata taacaaatct atttcataaa gcaaaaaagc ggctagacaa aagagatgtc
     4201 taaccactta catttatcat ctatataatt caat
//
LOCUS       DAXLPI010000159         4210 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXLPI010000159 DAXLPI010000000
VERSION     DAXLPI010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.163"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            184..459
                     /gene="rpsP"
                     /locus_tag="ACF3T5_002538"
     CDS             184..459
                     /gene="rpsP"
                     /locus_tag="ACF3T5_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFD3633061.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            471..722
                     /locus_tag="ACF3T5_002539"
     CDS             471..722
                     /locus_tag="ACF3T5_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFD3633062.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            847..1638
                     /locus_tag="ACF3T5_002540"
     CDS             847..1638
                     /locus_tag="ACF3T5_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633063.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            1808..2329
                     /gene="rimM"
                     /locus_tag="ACF3T5_002541"
     CDS             1808..2329
                     /gene="rimM"
                     /locus_tag="ACF3T5_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFD3633064.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2319..3080
                     /gene="trmD"
                     /locus_tag="ACF3T5_002542"
     CDS             2319..3080
                     /gene="trmD"
                     /locus_tag="ACF3T5_002542"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFD3633065.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3216..3563
                     /gene="rplS"
                     /locus_tag="ACF3T5_002543"
     CDS             3216..3563
                     /gene="rplS"
                     /locus_tag="ACF3T5_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFD3633066.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            3923..>4210
                     /locus_tag="ACF3T5_002544"
     CDS             3923..>4210
                     /locus_tag="ACF3T5_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633067.1"
                     /translation="MGKDKRIVWKDQSDLKIILTISQFIETYEIKSSREYQKQLSKNP
                     HSAPSMWFIINKYGSWNNLLNSIGIDNNGSKKWARMETDELIKVAQIFIDSE"
BASE COUNT         1481 a          728 c          836 g         1165 t
ORIGIN      
        1 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
       61 aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
      121 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
      181 ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt
      241 atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact
      301 tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg
      361 ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt
      421 atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt
      481 taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt
      541 tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata
      601 ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg
      661 gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt
      721 aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga
      781 agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa
      841 cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt
      901 tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc
      961 agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca
     1021 aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg
     1081 cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa
     1141 ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg
     1201 aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa
     1261 acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt
     1321 tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa
     1381 ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta
     1441 gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt
     1501 aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa
     1561 ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt
     1621 tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca
     1681 caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa
     1741 agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg
     1801 atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg
     1861 cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt
     1921 gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa
     1981 acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa
     2041 atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga
     2101 attctattac catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa
     2161 agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa
     2221 agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg
     2281 agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt
     2341 tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata
     2401 aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga
     2461 cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct
     2521 atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc
     2581 ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac
     2641 acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga
     2701 cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca
     2761 tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa
     2821 cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata
     2881 aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc
     2941 aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg
     3001 aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa
     3061 cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt
     3121 tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag
     3181 ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa
     3241 caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc
     3301 acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca
     3361 aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg
     3421 gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct
     3481 acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc
     3541 gtatcaaaga aatcagaaga taaacacgga aaacttcctt ggaaatctga aatctgtgaa
     3601 aagtccttgt atatcaagga ctttttcttt tttctctatc acatttttaa tatgttttgg
     3661 gccttttttt ctaatgttct ctcaaattgt cctgaatctc ccaatattga ctactattat
     3721 cgtcaaaata ccgaaaaaat tagtagtcat tttgacgaca aatttaatac gaaaaatacc
     3781 taaacactta ccgaatttag caatttttga acccgagttg ctaaaaaggt tgaatgcttt
     3841 cgaaaaaagc aaaacatata ccattgtgct aaaaagttta tgtgataggt ttctatttaa
     3901 ataattttag gagttttttg taatgggaaa agataagcgt attgtttgga aggatcaaag
     3961 cgatttaaaa attatattaa caattagtca gtttattgaa acttacgaaa taaaatctag
     4021 tcgagaatat cagaaacagt tatcgaaaaa tccacattca gctcccagca tgtggtttat
     4081 tattaataaa tatggctcgt ggaataattt gctgaatagc attggaattg ataataacgg
     4141 atctaaaaaa tgggctagaa tggagaccga tgaattaata aaagtggcac aaatatttat
     4201 tgattcagaa
//
LOCUS       DAXLPI010000160         4152 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXLPI010000160 DAXLPI010000000
VERSION     DAXLPI010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.164"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(132..590)
                     /locus_tag="ACF3T5_002545"
     CDS             complement(132..590)
                     /locus_tag="ACF3T5_002545"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFD3633068.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(761..1906)
                     /locus_tag="ACF3T5_002546"
     CDS             complement(761..1906)
                     /locus_tag="ACF3T5_002546"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFD3633069.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPHHELHGVAKQQMSGFSGMISFTLKNEETAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(1919..2836)
                     /locus_tag="ACF3T5_002547"
     CDS             complement(1919..2836)
                     /locus_tag="ACF3T5_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFD3633070.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3264..3827
                     /gene="efp"
                     /locus_tag="ACF3T5_002548"
     CDS             3264..3827
                     /gene="efp"
                     /locus_tag="ACF3T5_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFD3633071.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            3928..4098
                     /locus_tag="ACF3T5_002549"
     CDS             3928..4098
                     /locus_tag="ACF3T5_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633072.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEREETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1304 a          772 c          788 g         1288 t
ORIGIN      
        1 ataaaaagag cgctaaactg aaaactagct atttgttttc ccgtttagaa ctcttttcag
       61 acaataaata tttaataaaa tacgaagata agatagctat attgcttctc atctttacgt
      121 tcttttagaa actatgcttc tacttcagac cattcatcgc gttttgctaa aaattcacga
      181 gccaaagctt gtgcaccttc tagtgtatgg ctggcagccc agccacattg tgtttcattg
      241 cttgcaggta cttcagtagc ttcaagaaca tctttcattg ttgcttcaag aacatctaaa
      301 atgtcttcgt agctttcatg attcaataca gtcaagtaaa agcctgtttg gcatcccatt
      361 ggagaccagt ctacgatgta atctgcgtga ttgcggatta gttcggcagt caagtgctca
      421 agtgaatgta gacttggcat gtccatgtgt tctttatttg gttgtttaaa gcggacgtcg
      481 tatttaatga tgacatcgcc atgcgcaccc gtttttcgat cagctacacg tacataaggt
      541 gctttgactt ttgtatggtc aagattaaaa ctttcgacat tcattctcat ttgtaagact
      601 cctatctgtt tgttcattcg ttttgatgct tttcactaaa gaattattta gtgaaattta
      661 caaaaacgtc tttctacttg attctaacac ttatcgtttc caaggtgtag caatctgaaa
      721 tattttttag atttttctgc taagtcgttt attgggactt ctagttcttg attctgtcaa
      781 atgcctgagc taaatcgttt atgagatcct gaccatactc aattcctaca gaaagacgga
      841 tcaagccgtc ctttatgcca gcctcttctc gtttttcttt tggaatagac gcatgagtca
      901 tgacagaagg aatttccact aaactttcta cgccacctaa actttctgct aaagtaaata
      961 gttggagtga ttcaacaaat ggaatagcag tttcttcatt ttttaaagta aaagaaatca
     1021 ttccgctaaa gccgctcatc tgctgcttag caactccatg gagttcatga tggggtaatc
     1081 ctggatagta gaccttttct actgctggat gagaaaatag aaaatcagct accacaaaag
     1141 catttttttg atgttcctcc atacgaacac ttagtgtctt gattccgcgc tgaagtaacc
     1201 aactatcttg gggaccaagt acggctccga tagcattttg gtaaaatccg atctgatcgg
     1261 ctagatcttt atggttagaa gtcactaaac cagctactac atcgctgtgt ccgcctagat
     1321 atttggtacc gctatggaca acgatatcag aaccgagatc taaaggtctt tgaaaataag
     1381 gagtagcaaa tgtattgtca gcaataacta tcaatccatg ttgatgagcc agagtggcac
     1441 ttttttctaa atcagtaatc tttagaagcg gattgctagg tgtttcaaga taaagtgctt
     1501 ttgtattggg tttgatggat tgttctattt tatcaagatt actcgtatct atgatcgtgt
     1561 actcgagacc atttttagta agaactttgt caaatagacg gaatgttcct ccatagacat
     1621 catctcctaa taagatatga tcgccagctt gaaaaagaga aaaaacggca tgaatcccag
     1681 aaagaccaga agaaaaagcg aatcctcgaa caccgccttc caaatcagca atcagttctt
     1741 ccaatgcaaa acgagtagga ttaccagatc tcgaatattc atattgctta ggctggccga
     1801 cgccatcttg ccgataagtg gatgtttgat aaataggtac actgacagct cctgttgtgg
     1861 gatcttcact gatgcctccg tggatcaatt ttgtttgaat gtgcatgttt ctttcctcct
     1921 ataagtaaat gttttttgat aaatagcggt ctgctgcgtc tggaaaaatc gtaacgacac
     1981 gatgaccagg tggtaaacga cgaatttctc ttaatgctgc tgcaaaagcc gcaccactcg
     2041 aacttccaac taatagtcct tgttctcgtg ctaactgcct agtatagtta aacccttcta
     2101 catcagagat tgtctcgatc tgattgattg aaaggggaga aagaaacggc ggaatgaatt
     2161 ctacaccgat accctcaatt tcgtgagggg ctggatcacc accatttaac acagagccct
     2221 ctggttctac accgattatt cgaatgtcgg gatatttttc tttcaaaaag gtagaagttc
     2281 cagcaaaggt tccaccactc ccaattcctg ctacaaaact atggatattt tctttgattt
     2341 catgaaagat ttctggccct aacgtatgat agtaagcagc aggattatct ttattttcaa
     2401 attgaagagg cagatagctg tttgatatct cctctgcaag ttttttactt ttgctgatgg
     2461 caccgatgat tccttgctca ctaggagaat ggatgatctt tgctcccaat gctttcatca
     2521 attgttgttt ttctaagcta aacttctctg gaacaacaaa aaccgttctt aatttgtatg
     2581 tgagtgcggc cagagcgatc ccaattcctg tatttccggc agttggttcg atgatcgtcg
     2641 ttttttcatt gatttttcct tgggcaaatc cttctttgat caaataactg cccaatctat
     2701 ctttgatact tcctccagga tttaaatatt ctagttttgc ataaatagca gaacctttag
     2761 gcatagaata gtgctgattg tcaaagcgat aaagcggtgt ggaaccaata gcttctgtaa
     2821 ttgtcgagat aatcatatgg gcctcctaaa aaaagataat aaaaaaagga gctgactatt
     2881 aaatcagctc cttgaaagac atagagaaaa attgagtaga taaaaagata agttacctgg
     2941 tactcattct atgaagggca attaatagac atgacaaata gtatgctgct tttatgcacc
     3001 atacaacagc aacgattcaa ttgtttttgg ttgctgactg tcatgtgtat ccctccaagt
     3061 aaaattttta ttaacgatag catgaaaaaa aataaaagtc aaaggattga aagaaaaaaa
     3121 tatttcgata gcatcgttta cccgtgttta tttttctgac aaataggcat gagaccttaa
     3181 aagatagaga atactctagt tttctggccg gttgtctgat acaataaagt tgattatgat
     3241 aaagaaaaga aggagcaaaa ataatgatct cagcaagtga tttaagagcc ggtatgactt
     3301 tcgtacaaga cggcaaactg attaaagttc tggatgcaag ccaccataaa cctggtaaag
     3361 gaaatacagt tatgcgtatg aaactgaaag atgtccgttc aggtgcaaca tacgacacaa
     3421 cgttcagacc agaagaaaaa tttgaaaaag cacacatcga cacaaaacct gtacaatatt
     3481 tgtacacgat ggatgataca gctttcttca tggatttgga aacatatgag caatatgaaa
     3541 ttcctgtaga aacagtagaa gaagaaatga aatatatcct agaaaacatg gaagtaaaaa
     3601 tccaattctt cggtagcgaa gtcatcggcg ttcaattacc aacaactgtc gtattacgtg
     3661 ttgtggaaac acaaccttcc atcaaaggtg caacagtcac tggttcaggt aaaccagcga
     3721 caatggaaac tggcttagta gtcaatgttc ctgacttcat cgaaacagat gaattgttgg
     3781 aaatcaatac agcagaaggt tcttatgtaa aacgtgcgtc aaaataattc gctaacggat
     3841 tgataagaat ttgaaaacag agcttgacct cttgaaaggc agatcatcgg atctgccttt
     3901 caagaggttt tttgtggaat cagcaaattg aaaaataaaa aagtatttga ttttatttat
     3961 attttgaaag atgaatggca aaagctagag agagaagaaa ctgcacttat ttatgaagaa
     4021 attgaaatga ttcataaaat tgaaaatatc cgaactattc cgaactatat tgaagcgaaa
     4081 atattttttc atcactaaat agtgttgaat ttatgttttt gtgtataaat agcttctaat
     4141 agttcgggta tt
//
LOCUS       DAXLPI010000161         4031 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXLPI010000161 DAXLPI010000000
VERSION     DAXLPI010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.165"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            201..1097
                     /locus_tag="ACF3T5_002550"
     CDS             201..1097
                     /locus_tag="ACF3T5_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633073.1"
                     /translation="MEINLFEAGNRIKQIREKHGYTMADLGKLVDANSPSTINNWEKG
                     NNLPNRKRLEKIALLGGTSVEWIKYGDFSDYVYRLTQNIEEKILKEYGETTLNNYREA
                     LLDELTIEKITYEEDTKILAAAEKINTQNIFQEVKYMYSPDEDYRNIEVKPDLGLTDD
                     NKNIKMIGQRIRKIRKKENITLEEFGKLFSPTADKAVISNWENGKNLPNSERIKKIAE
                     IGGVSELYLMTGVDSSVAESMYFLSEVALDALERLSIEEVNRIIESFAGYLNVISKID
                     DPDKREISLNAICQLSLLDKMG"
     gene            1146..1520
                     /locus_tag="ACF3T5_002551"
                     /pseudo
     CDS             1146..1520
                     /locus_tag="ACF3T5_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353455.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1776..2528
                     /locus_tag="ACF3T5_002552"
                     /pseudo
     CDS             1776..2528
                     /locus_tag="ACF3T5_002552"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2542..3324
                     /locus_tag="ACF3T5_002553"
     CDS             2542..3324
                     /locus_tag="ACF3T5_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633074.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSNTFSLNKDYELLNPIIKNIFTKYYSKDFEDYRDIEVKPDPKKVGRKIRSIRK
                     KLGLTMQEFGYEVSNSPRSTVSTWEHGGNLPNKAKLKKIADIANCSVEDLLFDEGFIS
                     EKSQVKLINELKEENSKLKEKIKYYECLFDGIESLLNSRKNS"
     gene            3364..3975
                     /locus_tag="ACF3T5_002554"
     CDS             3364..3975
                     /locus_tag="ACF3T5_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3633075.1"
                     /translation="MLVGYARVSSDDQNLERQLEEFRKIGVEKVFAEKKSGRNTVERP
                     EFNKMLSFVREGDTLVFESLERLGRNSDEILETVSFIDKNGMGLMVLNLPILDTKFGD
                     PNLEKLVRSLVINIFSWTAQNEREEIKRKQKQGIAIAKKKGVYKGKPYEYSSTAKNPQ
                     KRFIYNEIVRMLGEGVPIKRIAEKTGTNRNLVYRIKKRISESD"
BASE COUNT         1593 a          547 c          748 g         1143 t
ORIGIN      
        1 ggttgaattg atagatggac tcaaagaaga aaattcaaag ttaaaagaga agataaaata
       61 ctatgaatgt ttatttgatg gtatagagag tctgctgaac tcacgaaaaa atccctagtt
      121 tttatgaatg accgttttgt aaatctgatt gaaatagatt tatatgattt cggtatatga
      181 tatacattag gaggtgtcga atggaaataa atctttttga ggctggtaat cgaataaagc
      241 aaatacgaga aaaacatgga tatactatgg cagacttagg taaactggtt gatgccaata
      301 gtcctagcac aattaacaat tgggaaaagg gaaataactt acctaataga aagcgtcttg
      361 aaaaaattgc actattaggt ggtacttctg ttgaatggat aaagtatgga gatttttccg
      421 attatgttta tcgcttgaca caaaatatag aggaaaaaat tcttaaagag tacggagaga
      481 ctacactgaa caactaccgt gaagctcttc tagatgaact tacgattgaa aaaatcacat
      541 acgaagaaga tacaaagata ttagcagctg ccgaaaaaat caatacacaa aatatttttc
      601 aggaggtgaa gtacatgtat tctcctgatg aagattatcg aaatatagaa gtcaaaccag
      661 atttaggact gactgatgac aacaaaaata ttaaaatgat tggacaacgt ataaggaaaa
      721 taagaaaaaa agagaatatt acattagaag aattcggcaa actattctct cctacagctg
      781 ataaagctgt tataagcaac tgggaaaatg gtaaaaacct tcctaactca gaaagaatta
      841 aaaagattgc agaaattggc ggagtttctg aactttatct tatgacgggt gtcgattcaa
      901 gcgtagcaga atcaatgtat tttctatctg aagtagcatt ggatgctttg gaacgtctgt
      961 caattgagga agtcaataga ataattgaaa gcttcgccgg ttatcttaat gtgatttcaa
     1021 aaattgatga tccagataag cgagaaatat ctctcaatgc tatttgtcaa ctatcacttc
     1081 tcgataaaat gggttgattc acaaaaaata ttgaaagatt actttactca cgaggaggct
     1141 tagctatgaa acctaatcta tcgttaacta gtgaacgtat tagaaaaatt atcgaatcca
     1201 aaggtctgac attagaacga ttcggtgatt taattggcga cacaccaata agttctgtta
     1261 ataattggct tagaggcgtg tctttaccca ataaaaatag gcttttaaca attgcaaata
     1321 ttggaaacac tagtgtagac tggattaaat ggggaacttt ggaagaatac atagcttcat
     1381 accttataga ttcaggttat gagctatata ttaaagactt tccagagata ccttacaaag
     1441 tctttaaaga cattcaagaa aaatatagtg atacgttttc actagataaa gactatgagt
     1501 tattgaatcc tataataaaa tttactatag ggggcgttac tttgaataga ataaaatttt
     1561 atattgattc cagtaaaaag accattaaac aaattagtac agaaactggt attaactata
     1621 caactcttac gaactattac cgtggtgagc gaaatcctag aaatactgaa acatggaata
     1681 tccttgctga ttatttccat gttcctgtaa gttatttgat gggattagac gaaacaaatg
     1741 ttgaactcaa aaaccttaat attggaaaca acatcaaaaa tattagaaaa agtaaaaata
     1801 tgacacaaac tgattttgct gagttaatgg gtctatcacg atcatatata ggggatttag
     1861 aaaacaacag gagtaatccc agtataaaaa cactggaagc tattgcaaga gcattggacg
     1921 ttccgatagt agcattaatc aacgattctt ctactgataa tatagtagag ccgtatgaaa
     1981 gtttgacagc cgaaaatatc cttcttaaac aagaaaatga ggaattaaaa tatgaggtag
     2041 aaagcttacg aaaaagattg tatgaaatcc aacaagtagt aaatcaagga gctgaaaaaa
     2101 tgattcccaa tctaaaagac gtaagtaaaa gaatcaaata tatcagaaaa aaattaggta
     2161 tgactatgga gcaatttggt tctcatttaa gcaactcacc taaaagtaca attgctactt
     2221 gggaaagtgg tagaaatata ccttctcaga aaaagttaca gctgattgct atagtaggag
     2281 aaaccaccac tgactggatt aaatggggaa ctttagaaga gtatataact tcatacctta
     2341 tcggtatagg atatgagcta tatattaaag actttccaga gatacctcac aaagtcttta
     2401 aagacattca agaaaaatat agtgatacgt tttcgttaga taaagactat gaattattgg
     2461 attctataat aaaaaatata tttacaaaat attactcaaa agaatttgaa gtaacccaaa
     2521 ttccataaga gaggtgattg tatgaagcca aatccttcag aagcaggaaa acgaatcaaa
     2581 caattacgcc tttcatgtgg ttttactatg gaagaacttg gaaggaaaat agataattca
     2641 ccacgtgcga ccatatccaa ctgggaacgt gggaccaatt taccaaatcc acaaaagctg
     2701 aaattattat caactatcac taattcaaca attgactgga ttaaatgggg aactttagaa
     2761 gaatatataa cttcatacct tatcgatata gggtatgagc tatatattaa ggactttcca
     2821 gaaatacctc acaaagtctt taaagacatt caagaaagat atagtaatac gttttcgcta
     2881 aataaagact atgagttatt gaatcctata ataaaaaata tatttacaaa atattactca
     2941 aaagattttg aagattatcg agacatagaa gttaaaccag accctaaaaa ggtagggaga
     3001 aaaattcgtt ctattagaaa aaaattagga cttactatgc aggaatttgg gtacgaggtt
     3061 tctaattctc ctagaagcac tgtgtcaaca tgggaacatg gtggtaactt accgaacaaa
     3121 gccaaactaa aaaaaattgc agacattgcg aattgtagtg tagaagattt gctttttgat
     3181 gaaggcttta tttctgaaaa atcccaagtt aaattgataa atgaactcaa agaagaaaat
     3241 tcaaagctaa aagaaaaaat aaaatactat gaatgtttat ttgatggtat agagagtctg
     3301 ctgaactcac gaaaaaattc ctagttaatt aaactagagt aaaattattt gtaggtgtta
     3361 aatatgttag ttggctatgc acgagtcagt tcagatgatc aaaatttgga aagacagctt
     3421 gaagaattta gaaaaattgg tgtcgaaaaa gtatttgctg agaaaaaatc tggacgtaac
     3481 acagtagaac ggccagagtt caataaaatg ttgtcatttg tcagagaagg ggatacgctt
     3541 gtttttgagt cgcttgaacg cttaggaaga aattctgatg aaatactaga aacagtatct
     3601 tttatagata aaaatggtat gggcttaatg gttttgaatt taccaatttt agatacaaaa
     3661 tttggtgatc caaacttaga aaaacttgtt agaagcttag taattaacat tttttcttgg
     3721 acggctcaaa atgagaggga ggaaattaag agaaaacaaa aacaaggaat agcaattgcg
     3781 aagaagaaag gagtctataa aggaaaaccc tacgaatatt cttctactgc aaagaatcca
     3841 caaaagaggt ttatctataa tgaaatagta agaatgcttg gagaaggtgt tccgattaag
     3901 cgaattgcag aaaaaacagg aacaaataga aatttagtct atagaataaa gaaaagaata
     3961 agtgaatctg attgatttta agttctttga gaaatcaatg ttacgagaga aggattctaa
     4021 gcctgtttca a
//
LOCUS       DAXLPI010000162         3927 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXLPI010000162 DAXLPI010000000
VERSION     DAXLPI010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.166"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..787
                     /locus_tag="ACF3T5_002555"
     CDS             <1..787
                     /locus_tag="ACF3T5_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFD3633076.1"
                     /translation="FFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESIS
                     ELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKE
                     YKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETI
                     DLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKH
                     EHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            798..2907
                     /gene="topB"
                     /locus_tag="ACF3T5_002556"
                     /pseudo
     CDS             798..2907
                     /gene="topB"
                     /locus_tag="ACF3T5_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            2923..3072
                     /locus_tag="ACF3T5_002557"
     CDS             2923..3072
                     /locus_tag="ACF3T5_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633077.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3153..3749
                     /locus_tag="ACF3T5_002558"
     CDS             3153..3749
                     /locus_tag="ACF3T5_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFD3633078.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            3762..>3927
                     /locus_tag="ACF3T5_002559"
     CDS             3762..>3927
                     /locus_tag="ACF3T5_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFD3633079.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIG"
BASE COUNT         1434 a          681 c          849 g          963 t
ORIGIN      
        1 attctttgaa agtagtattg atctgttaag ttacgctgca ttgaatcggg agaaactaca
       61 agatgcctgg ttagtttcga tggacgggtt aaaacatcat gtaatcagtc attacgttga
      121 agagtcgatt tctgaattaa gacgaaaaca aacatttcct caatcgattg aaatatgtgt
      181 ggacaatgat cgtgcaggac atatttttta tgaaaaagaa cagatgaagg gaatcgttga
      241 tccttttacc aataagaaaa tccgttgtga gcgaggaatt ccaaatgatt ggcaagtacc
      301 taaagaatac aaagctactt atgaagcagt cgcaaaagaa atgagtgttg aacctgaagc
      361 aattatggcc atacacaaaa ctgaaactaa tttacagctg acaaatcaat tggtgtctgc
      421 tcatgatgtt caatctacgt ttggaaagat gttggcgaag ggcgaaccag tagaaacaat
      481 tgatttgaaa gaagcatgta ccacggtcgc gaaggaactg aaagtatgtg aacgcgcaga
      541 tggaacatac aactttgatc gtttttatag tagaaaagcc aacataaaag acgttaatgc
      601 aggaatcctt ctttcctata aagcagaaca atactacaaa ggctataaaa agcatgaaca
      661 tgaatttgta cctgaagtga aaaaggattg gaatgatcaa ttgaagcatg agatccagca
      721 acaagaaatc agaaagcaaa aacgtgcgat gttgtttcaa caaggcagac aacaagaaag
      781 ggagtgaagt cgtcactatg aaaaccgtca ttttagcaga aaaaccgtca caagcgaagg
      841 cttatgccga tagcttttct aaagctaccc gaaaagatgg gtattttgag atccaagatc
      901 gattgtttac aggagaaact gtgatcacgt atggttttgg tcatttagtc gagttagatt
      961 cgcccgatat gtacgatgaa aaatggaaac aatgggcatt agaacatttg cctattttcc
     1021 ccaatcaata ccactatcat gttcctaaag ataagaaaaa gcagtttagt gtcgtcaaac
     1081 gacagctgca gtcagctgat acgattgtga tcgcgactga tagcgatcgt gaaggggagc
     1141 tgattgcctg gtcaatcatt caacaagcag gcgccaatca aggaaaaacc tttaaaagac
     1201 tttggatcaa ctccttagaa aaagaagcca tttatcaggg attccaacag ttacgagacg
     1261 cagaagagac ctatccaaaa tttgaagaag tacaagcacg tcagatcgcc gattggttaa
     1321 ttgggatgaa cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa
     1381 gtttttcttt aggtagagtc caaaccccaa cactctatat gatttatcaa ttgcaggaaa
     1441 agattagaaa cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa
     1501 atggcgcatt tgatgcgaag cttgatccaa atgaaaccca agcgacacaa gaagcatttg
     1561 aagcctattt aaaagaaaaa ggtgttcaat taggaaaaca gccaggtacg atccttcaag
     1621 tcgaaacaga gaaaaaaagt gcggctagcc cacgtctttt ttctttatcg agccttcagt
     1681 ctaaaatgaa tcaactcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt
     1741 tgtatgaagg aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg
     1801 aatatgcgta tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga
     1861 ttccgactcc tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac
     1921 attacgcgat cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc
     1981 cactgcagca ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa
     2041 agtataccta tgaagaaaca acgatcttga cgcaagttca gcagcttcag ttaaaggcaa
     2101 tcgaaaaggt gccgctagat ctaggttgga agaagttatt tggaaaagag agcgacggaa
     2161 aagagaagga agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtgg
     2221 atctacaagt tcttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga
     2281 tcactgcaat gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga
     2341 aagaagtgga aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac
     2401 aaaaggaata tatcaaggta gagaaaaata agctcgtggt gacgaataaa gggattttgc
     2461 tttgccaagc cgtggaaaaa gagccgctgc ttacgagcgc cgaaatgacc gccaaatggg
     2521 aaagctactt gttaaagatc ggtgaacgaa aaggaacaca aaccactttt ctagccaata
     2581 tccaaaaatt tgtctctcac ttattggaag tcgtaccagg gcaaatccaa tccactgatt
     2641 ttggatcgac gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt
     2701 taggaatttg tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt
     2761 gtacgtctga agcctgtgat tttaaaaggt cagaagggtt catttgaagc aacgattgtt
     2821 ttaaaagaag acttcactac aggctttgaa ttttctgaaa agaaaaaaac gaacttcaag
     2881 aaaagaacac gccgcactgc aaaataatgg agggttaatc ttatgcgtaa taccacacac
     2941 aaacaacaag aaatcgaaaa tcaaaaagcc aaaattcgtt cagaacagga aaaaatgcgt
     3001 gccagcaaac gaagactgca gcaattgaag aaccagcaga ggattcaaaa agaaatcgaa
     3061 ttagatcgat aaaatcttcc taactattgt atacgtataa acttatgtta tactgttttt
     3121 ataagtttat acgtatacaa aggagggaga aaatgacaaa agcaatggta tacaagctgc
     3181 tagaagaatt taatggaact ttggctttga aggatgccaa aaaagcgggc atttctcctg
     3241 taacgatcaa acgtatggta gatcgagggg agctggatcg agagtatcca ggttttttca
     3301 ccttacctgg tcaatttcct gatgaattgt ttatggcgca aaaaaaatat gaacgcggca
     3361 ttatttcaca tattaccgcg ttagatttgt atgatttaac agatatgatc cctagacaga
     3421 tcgatctgac tgtaccttac ggttatcacg tttctgaaaa aggattgaag gaattcgcag
     3481 ttgagctaca ctatacaaaa gcggaatggt atgaattagg taagattgaa ataaaaagtc
     3541 gctatgggaa tcctattata gcttatgatc cagaaagaac tttatgtgac atttggaatc
     3601 cctggtatac cgttgaggat gaaatcaaag taaaagcaat caaaaactat atggagtcgg
     3661 acagaaaaaa tcttagaaaa ttgaatgagt atcgacgaat tttaccaaca gacaaaacga
     3721 tgcgttctta tattatggct ttaaattaaa ggagaagata catgacacct acgcagttaa
     3781 aagcaaaagt gaagaatatt tctagagaaa aagaagttga tccgcagctt gttttaagac
     3841 attttatgat ggaaaaattc ttggaaaaaa tctcagagtc tccttatcgt gaaaattttg
     3901 tgttaaaagg cggcttcttg attggct
//
LOCUS       DAXLPI010000163         3926 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXLPI010000163 DAXLPI010000000
VERSION     DAXLPI010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.167"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(112..387)
                     /gene="prgO"
                     /locus_tag="ACF3T5_002560"
     CDS             complement(112..387)
                     /gene="prgO"
                     /locus_tag="ACF3T5_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFD3633080.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(359..1312)
                     /locus_tag="ACF3T5_002561"
     CDS             complement(359..1312)
                     /locus_tag="ACF3T5_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3633081.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1924..3417
                     /locus_tag="ACF3T5_002562"
     CDS             1924..3417
                     /locus_tag="ACF3T5_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFD3633082.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3552..3848
                     /locus_tag="ACF3T5_002563"
     CDS             3552..3848
                     /locus_tag="ACF3T5_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFD3633083.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
BASE COUNT         1307 a          646 c          703 g         1270 t
ORIGIN      
        1 tgttgtatac ccccaaacaa tacacatatt ttgaaggatt aggattaata aagctaaaaa
       61 tatagtaagt atactaaata tatttactat atctagtata cttactatat tttaatcttt
      121 tgatcgtcta ttatatataa tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat
      181 tgcaagtttg actatttcat acataggaat atcttttgca taactaagat cccgaatggt
      241 ttttagtacg ggaggatcta cttgaactgg ttttctttct ttcgcagtgt actttcgttc
      301 ccctaactcg acattaatat catctttaat tgaaatatct tcagcaccaa ttttcttctt
      361 atctttgttc gttggtaata atgccattta gtttaatctc ctttccaaga ggtaggatat
      421 gattggccat tgagtcagca tatttaggtg tataatcaaa tccttcaata tcaccatgtt
      481 gttcatagta ttcaattctt tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc
      541 atcttcgatc tacataatca tttaattgga taccattctc accatacatt tctaaccgat
      601 ccgagccttt aacaatgctt ttaaaaatgt ttgattcacc atacttatca attgtttttt
      661 gatagtttgc gtgttgagag gaacgtcgca ctgagaaaag aacaggcaaa attccaatga
      721 tttgaagatg agactcttca aaattcgtga caatcggaac taaaacttta ttaataaaat
      781 cttctgttcc ttccattgcg taccgtttta attcttgcat tgcaataata taatctgcag
      841 cagttaagaa tgcacgaacc acattatctg ttgatggtgg gcagtcgata aagatataat
      901 cgaaattttt tctcaattct tctaaaggtt taatgaaggc caatgatctc ttacgacgat
      961 caggatattt ttcggacaag tattcattaa gttcttcaga tccaggagag ccagccataa
     1021 tatataaatg tggacttact tgagtaatac ctgaaaccca gtcccctttt ttgattgctg
     1081 cagacatgga tcgatcaaag ctagtaattt gatacgttga tccaatgttg gaagtagtat
     1141 ttctttgttg gtcataatca attagcaaca cgtctttttc aaaaagttga ctagaaacaa
     1201 tcgctaataa atcattgtct gtagtcttac caatgccgcc ttttctacct gtaagcatta
     1261 caacttttcc tagtttatct gatgttttaa atgcagtccg taagttttcc atagttccct
     1321 ccaatttcct atactaaata tatttagtat acttcatata cttaatatat tcgatatatc
     1381 taatatactc caaattgata agaattcaga ctttataagg aatatagtgg atatactaaa
     1441 tatatttgct atattcagta tatcagatat attcaaaatg tcaaaatata tttgtgcgtt
     1501 ttgcgttgac aatttagagt gaaaagaggt aaacttaatt ctatagaaac cacagataat
     1561 tcactattta caaagtagca aaagcagtgg tactctttaa acagaaaaat tacataaaaa
     1621 aaagagacgg aaataattga ttttagcaga atcaattatt tctgaatcac atggttagga
     1681 catgtgttca cgctcgtctc aagaatatgt aagtagctac caactactta tattaaaagt
     1741 tgatcggata ccttttccga ttgcctttat tctaccaaaa aaaggcgagc aactcaacag
     1801 aagaacgttt atttagtgta cttaaattaa atattcttaa acactgaaga gagaacacat
     1861 actatggctg agattaaagc taaactgtgt tctctttttg tttgagaaag gacgttacaa
     1921 tcgatgttga gcgaaattta ttcagctatt cttaaaaacg gcttacgtac atacaaatat
     1981 cgaaacagca ccatgaaacc tgcaggtcat attgattcca gtaggagtgg tgctatcttt
     2041 gcgtaccgta gcaagcaatt aatgaatgtt gggcgtggta tggtcattac ttcggaagaa
     2101 gctattttag agaacgaaaa gaaactaacg cattggacac ctaataccta tcgctttggt
     2161 acttatgcgg atgattatcg aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa
     2221 atcaacacat ttgtagttga tatcgattca aaagagaacc atcaaggcga aattatttta
     2281 gcctgtttag atcaagtggg ctacatgcca acgctaattt tagaatcaga tcatggttat
     2341 caagtttatt ttgtactaaa gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg
     2401 atcgatgtag caaaaaaaat ttcaaaaaca attcgtctac agttggctga aaaattgtcc
     2461 ggagtggatc taggatgtaa ccacttcgga attgctcgat tcccacaaga aaataatatt
     2521 gtttttaaag aaatgaacta tcagtattct tttgctgact ggattcaatg gtcgatgaag
     2581 cttgaatcag atcaaaaaac aaaagaagat cgagaacgaa aactaattgt ctttcctgag
     2641 aaaaaagaaa ttcgtcaagt agatgaaccg tggttcgata tgttacttca taaagcaaac
     2701 atcgtcggcg gagaaggacg tcttggtcga aataatgtga ttttcacgct ttctttagct
     2761 tattactctt caggttatgc attggatact tgcgaataca atatgttcac attcaacgaa
     2821 cggttacagg aaccgctatc tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct
     2881 ggtaattatc aagcagcaag cagagaattc attattgaat tgtgccagca atgggtttca
     2941 ccaaccttaa aaaataatga tctgttcatt caacgacgtg gctggtggaa atttaaaaag
     3001 ccacgagatc aaagaaaata tagtcataaa aatgaatgga aagaagatct attggcttat
     3061 ctaagcgaaa aaagtcttgc tagtgagcct tatttgaacc tgtcaaaaaa agaattggct
     3121 tcacaactaa cgatgcccaa gcgaacactt gataagatct taaatgagtt acgagaggag
     3181 aataaagtct tctacaaggt aacacgagga agaaatggcg gaatgatcat tgcttcagtc
     3241 aaaacgttgt tcgcgagggt tattcaacta agtaaagaaa agagactaga ttattcaaca
     3301 aggatcacag aggtatttgg actcaacgag agcttagtag aagagacatt tagacaatta
     3361 tcaaccccct tgaaccctct taaagaagtg aatctttttg aagtagatac aggttgaaaa
     3421 ataaaaggtg caaattgcct tatacaatat atctatgata cttctgtagg tgttgctccc
     3481 tttgcctagc tacgtgtagg tggtatactt ataactgcgt atgcagtctt atgtatcatg
     3541 gaggaagttg tatgtcgtta aaaaattatg tctattcaca tcctgttaat gtgtttatca
     3601 ttggtcgttt gggattatct gtggaagtgt tctgtgagct ttatggtttt aagcagggga
     3661 ctttgtcttc ttgggttacc agggaaaaaa cagttgcttc tttgccgatt gagtttcttc
     3721 atgggttgtc cttagcttct ggggagacta tggatcaggt atatgattgt ttgtgtgtgt
     3781 tagagcagga gtatattgag tataaaatag ctaacgagtt aagaaaaagg aagaagtata
     3841 tccagtagta gtttttattt actcttaatt gtcaaattaa gttagacaaa tcatcaatac
     3901 ttacgtaact caaaaatact tccaaa
//
LOCUS       DAXLPI010000164         3654 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXLPI010000164 DAXLPI010000000
VERSION     DAXLPI010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.170"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..776
                     /locus_tag="ACF3T5_002564"
     CDS             <1..776
                     /locus_tag="ACF3T5_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633084.1"
                     /translation="THEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLK
                     NAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYG
                     CEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSF
                     KIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGR
                     SVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            883..936
                     /locus_tag="ACF3T5_002565"
                     /pseudo
     CDS             883..936
                     /locus_tag="ACF3T5_002565"
                     /inference="COORDINATES: protein motif:HMM:NF019662.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            970..2346
                     /gene="tet(L)"
                     /locus_tag="ACF3T5_002566"
     CDS             970..2346
                     /gene="tet(L)"
                     /locus_tag="ACF3T5_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFD3633085.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            2910..>3654
                     /gene="mobV"
                     /locus_tag="ACF3T5_002567"
     CDS             2910..>3654
                     /gene="mobV"
                     /locus_tag="ACF3T5_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFD3633086.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGL"
BASE COUNT         1163 a          555 c          754 g         1182 t
ORIGIN      
        1 cgacacatga aattatactt tctttcttag ggaaagtaca aatggaagtg attagtgcac
       61 tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc atttatatgg
      121 agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca aatcctttct
      181 gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg cagtatgaga
      241 gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg gaagggatac
      301 gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa atctgtttta
      361 agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg cttgctccta
      421 ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca tatcttagtt
      481 ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct cctaaatatt
      541 gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt ggagaaatcc
      601 ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat ggacgtagtg
      661 tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt tgccagcccc
      721 gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata acttagtgta
      781 ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt ttttaataaa
      841 gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gaaatgaatg taacagggtt
      901 caattaaaag agggaagcgt atcattaacc ctataaacta cgtctgccct cattattgga
      961 gggtgaaatg tgaatacatc ctattcacaa tcgaatttac gacacaacca aattttaatt
     1021 tggctttgca ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta
     1081 cctgatattg caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc
     1141 tttatgttaa ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc
     1201 atcaaaaggt tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt
     1261 gttggccatt ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca
     1321 gctgcatttc cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg
     1381 ggtaaagcat ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg
     1441 attggtggaa tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata
     1501 acaattatca ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt
     1561 cattttgata tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt
     1621 acaacatcat atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta
     1681 aaacatatca ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct
     1741 tttatgattg gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct
     1801 atggttcctt atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta
     1861 attattttcc ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt
     1921 gatagaagag gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt
     1981 ttaactgctt cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt
     2041 gttttaggtg ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg
     2101 aaacagcagg aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag
     2161 ggaacaggta ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg
     2221 ttacctatgg aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca
     2281 ggaatcattg tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat
     2341 ttctaaatcg ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa
     2401 caggaattca aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga
     2461 gacctctctt gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa
     2521 gacaataaaa attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa
     2581 aataaaaatt ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac
     2641 tacctgtccc ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg
     2701 gcagagggca ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt
     2761 ttatggtttt ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag
     2821 tatagtgtgt tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt
     2881 tgtgccacct aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag
     2941 tgaaatcagc tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa
     3001 cgaatgatga tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa
     3061 atattgatta caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa
     3121 aaacgaggaa agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt
     3181 ttgagcaact ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt
     3241 ccgaacgata tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc
     3301 ctcacatgca tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt
     3361 ttaatcgtca agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg
     3421 gttttgagtt gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat
     3481 ttaaaaaaca aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa
     3541 aagatgaatg gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac
     3601 gacacatgaa aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgg
//
LOCUS       DAXLPI010000165         3652 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXLPI010000165 DAXLPI010000000
VERSION     DAXLPI010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.171"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..885
                     /locus_tag="ACF3T5_002568"
     CDS             <1..885
                     /locus_tag="ACF3T5_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFD3633087.1"
                     /translation="SLVDMYVSDYFLKAEASKCITGLYTANEFNHVTPKQLDDKSVVI
                     LCSHGGNTAETVKAGEVAQARGAVTVGLTHNKTADLLKVSDHSFLYEWGDDAKIVNNP
                     MAIILDLTVSLVNVTETFEHYGKFREGMKVIDTVIENGIKQVQDRIKVFADKYKDETM
                     FYILGSGPSYGHAYGFSICSLMEMQWLDSTSVHSGEFFHGPFEVTDKDTNFILLMSIG
                     RTRALDERAKRFLDKYAERVEIVDAKELGLDMISDEVSEFFNPILFYSILSEYRSALA
                     DVRNHDLDVRRYMGKVEY"
     gene            953..1711
                     /locus_tag="ACF3T5_002569"
     CDS             953..1711
                     /locus_tag="ACF3T5_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFD3633088.1"
                     /translation="MYPGGNSLNFAVFAKKLGHESSFMGVFGSDEEAAHVLSAIKEIG
                     LDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREYPIQLTEEDREYLNQFELIH
                     MGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAIDKVDFGFFSIEDFSDEEVKQ
                     FLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEEPVDTMAAGASFLTAFLLYF
                     LEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1779..2489
                     /locus_tag="ACF3T5_002570"
     CDS             1779..2489
                     /locus_tag="ACF3T5_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFD3633089.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2525..>3652
                     /locus_tag="ACF3T5_002571"
     CDS             2525..>3652
                     /locus_tag="ACF3T5_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFD3633090.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1208 a          548 c          814 g         1082 t
ORIGIN      
        1 tcgctggtag atatgtatgt ctcagattat tttcttaaag ccgaagcatc gaagtgtatt
       61 acaggcttat atacagcgaa cgaatttaac catgtaaccc caaagcaatt agatgataaa
      121 tctgttgtca ttctatgttc tcatggagga aatacagcag aaacggtaaa agcaggagaa
      181 gtcgctcaag ctagaggagc tgtgactgtt ggtctgactc ataataaaac tgcggattta
      241 ttaaaggttt ctgatcatag tttcctttac gaatggggag atgatgcaaa aatagtcaat
      301 aatcctatgg ccatcattct tgatttaact gtatctttgg tcaatgttac cgaaacattt
      361 gaacattacg gtaaattcag agaaggcatg aaggttatcg ataccgtaat tgaaaatggc
      421 attaagcagg tgcaggatcg tattaaagtg tttgctgata agtacaaaga tgaaacaatg
      481 ttttatatct taggtagtgg accatcatat ggacatgctt atggtttttc aatttgctct
      541 ttgatggaaa tgcaatggct tgattcaact tctgttcatt ctggagaatt cttccatgga
      601 ccttttgaag taacagacaa agatacgaat tttattctct tgatgagcat tggacgcact
      661 cgtgctttag atgaacgggc aaaacgattc cttgataaat atgcagaaag agtagaaatc
      721 gttgatgcaa aggaactagg attggatatg atatcagatg aagtttctga attcttcaat
      781 cccattttat tctacagtat tctttcagaa tatcgttcag ctttagcaga tgtacgcaat
      841 catgatttag atgttcgtcg ctatatgggg aaagtggagt actaggagga ttaaaatgaa
      901 ggtattaggc ttaggtgata acgtcgtcga taaatatgaa aatctgggaa ttatgtatcc
      961 tggaggaaat tcattgaatt ttgcagtttt tgcaaaaaaa ctgggacatg aaagtagctt
     1021 tatgggagtt tttggctcag atgaggaggc agctcatgtt ttatcagcaa taaaagaaat
     1081 aggactggac aacagtcact ctcgctttga aaaaggcgag aatggctgtg ccagagtgaa
     1141 gattgatgag ggcgaccgga ttttccttgg cagcaattcg ggaggtgtta ctcgtgaata
     1201 cccgattcag cttactgaag aagacagaga atatttaaac cagtttgaat tgattcatat
     1261 gggattatat agccatgtca atcatctatt agaagagtta caaaatgttt ctggaaagat
     1321 ttcttatgat ttttctgacg actttacgga agaagaggtt cagcgggcaa ttgataaagt
     1381 tgattttggc ttcttttcta tagaagactt ttccgatgaa gaagtaaagc agtttttgat
     1441 gaagcatttt tgtgagcgaa atgatgtatt gattgcaacc cgaggagaaa aaacggctat
     1501 tggttatgat ggagcaaagt tttatgaagt gaaccccgtg ctagaagaac cagttgatac
     1561 aatggcagcg ggagcttctt tcttaacagc tttcctactt tattttctcg aaggggacga
     1621 tatagtcacc gcgatgaaaa aggggaatga atttgctggg aagtcttgtc tggttgacgg
     1681 ttcgttcggc tttggtattc attacgattg agcttgtttt aaattcctgc atttgataac
     1741 ctaaagaaag aaatcccggt aaagtggagg ttaaaaaaat gctggataca aatgcacaat
     1801 taccattgta cttacaatta aaagaaatat taaaaaataa aatcaataca ggggagtatc
     1861 ccgagaatac gaaaattccc gctgaatcag agctgataga aagttatgag gtcagccgaa
     1921 tcacagtgag aaaagcagta gaagaactgg tagaagaagg gtacctactt aaaaaacaag
     1981 ggaaaggaac ttttgtcaac cggcataaag tttttcgtaa aattgaatat attatgggtt
     2041 tcagtgagag ttgtaaatct aatggtttta caccgactag cattgttttg gaacgcaaga
     2101 tcattccagc aactgaaggg atggcaaaaa aactcgatat cgctcaaggt gatgatgttt
     2161 tatatatcca gagaaagcga atggcggata acatccccat tatgttagaa aacaattatt
     2221 tttcttatga aagatatagt ttcttaatgg aggaagagct gactgattct ttgtatgaag
     2281 tacttaaagt ccaaggtacc ttagccatca atcctgggga aactgcactt gaaattgtca
     2341 cagctgatga ccaaattgcc aaaataatgg aagtagctgt tgggacgccc tttttcttta
     2401 tggatacttt aatcaacgat caaaacaaca taccaattca tgtaggtaat caatattacc
     2461 tgggagaagt atatcgtttt tacttatgat ttcctgtttg gatttcaagg agtagatttt
     2521 atttatgaag caaatattgc ggtcaaaaat gatttttgat ggagagcagc cacctttcaa
     2581 aggttatatt gtaatcaatg aagggatcat tgaaaaggtg gaaaacaact ggaattacag
     2641 tgatctatta aatgaaaaca cggaattaat tgcttatgat gatcaattcg ttatgccagc
     2701 aattcatgat aatcatgtat ttttcagtgg ttatttggct atgaatgctg gcatagattt
     2761 aagcgataca tcttcagcta acgaggctgt agagcgtatt aaagctgtaa tgaaccataa
     2821 aaaagcagat gaaccaattt atgctcatgg ctgggatgct gccctgtgga aaactgaccc
     2881 atcagaagag ctattgaatg aaataaacca ctttggtcca atcacagcga tcgacaaaaa
     2941 tcgcagccat tgttggatga atgctattgc taagcagact tatggtttta cagaaaaaga
     3001 aaccagtgct gaaagtcgag tattattaat taaggaaatg ctagcaaaca aaaagctttt
     3061 agcagaggtt tacgctgaat ttgaagcatt actgctttca aaaggtgtta tatcaatgaa
     3121 agaaatcgtt tttgatgacg cagaatttct ttctaatata cctcagagga aaatacttag
     3181 taacctatat gtgcaggcag tccaagaacc tctacagcat gaacttctat atgagtataa
     3241 ggaaaaagtt ttccctgaaa aggtccgctt tggtggagtg aagatcatgg tagatggtgt
     3301 tgttgcggat agaagtggag atatttacgg tgtttatcag tcaggagtag cttcacccga
     3361 gattgactat gaggcaatta aagcagaagt tgctgcattc aatgaagtag agattccttg
     3421 ctgtttgaca acggaaggag ataaagcggg ttataaagct gcgtcaattc ttgtcgaatt
     3481 tggaaagcgg ttacctgaag gcgtttttaa ttcgattagt gatttggaaa tggttactca
     3541 aaagacagca gaaaaaatga acgaaggaca agtagtagca gaggtatatc cacaaattct
     3601 cggcttgaat caaacgttag aagaggctta catgtcaact gttataaaaa at
//
LOCUS       DAXLPI010000166         3624 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXLPI010000166 DAXLPI010000000
VERSION     DAXLPI010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.172"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            718..2268
                     /locus_tag="ACF3T5_002572"
     CDS             718..2268
                     /locus_tag="ACF3T5_002572"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFD3633091.1"
                     /translation="MSDKNTYARRSQRHKQEPDSCENTNQLNEAVKPQTGNENKQPKI
                     PKKKSNYSKYIFALFTALILVIVVTGGYIVYTLKQQEAEAQAKYETAAKNLMASIQEE
                     QDQSGISTKIDTINDGENKCLVYRPVDENTVPFKNANQLLDELAQKQQKKHREKEVLT
                     VARIKATAISSKVNQYRIEADSFIWDRSKENFKKPDSISEKDIYIFKKTGKEITNKDL
                     FPDEGSLLGIQQVIQQSILDQAKEPEKIIDAVLTMDRISYDSKMTYTPDELTIVLPKN
                     STGTSKVTLKYKDIAPFIDTDLVSQESIKDTLPALDENKKYVALTFDDGPNNSSTLDL
                     LNILKTDNVKATFFMLGQMVDQNPDVAKQVHDEGHEVACHSYSHPQLNTLSTDELQSE
                     INKANKAIFKATGVLPRNIRPPYGAIDKKSAETIGMPIIQWDIDSQDWKSKNPRAINN
                     VVKQNVYNGAIILIHDIHPESVKAIPNMISMLKNKGYEFVTIDQLLSGKQKPLHQYFG
                     MNDERLVD"
     gene            2553..2772
                     /locus_tag="ACF3T5_002573"
                     /pseudo
     CDS             2553..2772
                     /locus_tag="ACF3T5_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1377 a          529 c          571 g         1147 t
ORIGIN      
        1 tactattaat tgtgattttt aaatgattga aaaatagaga ggaggaagca atacgtagaa
       61 aaaatatttt aaattggtgt aagtacaaat atgttattta cagtatttta gtttctcgta
      121 ttaagtgtta ctactatggt gaattcttta gagaattaat aaatagtctt ctttgataga
      181 ttgttaaagg gaacttaaca aagtaataag atgcataatc aaatgcacaa aaaacaaaaa
      241 aagaagtttc ttcgaataga aattctgaaa taacatcctt tcgcctaata gtgaataatt
      301 taaataaaga gaaactggct acactatcaa agcaagtgat tctgtttagc taattactaa
      361 taattaaagt attaaaatag tatgtttaat caaatagaac aagattggat aatagtttat
      421 atttttaatc ctagaccatt attggtattt caaagactac aaccatacta attaatctat
      481 cgaatggtac aatttcaaaa actaaaatca aaactagtga ttctaatgtt atcaaaactg
      541 agttgaaaat taagtagcca caatatctaa agtggtgagg aattagcgat aattactacg
      601 ccgaaagtaa aagaataata gaaaaaactt tccttaaaaa ttatctatga attcgttttc
      661 attataattg cgctgtttta ttttacttta tgatgataaa tggagggata aagtcatatg
      721 tctgacaaaa atacctatgc acgtcgtagc caacgtcaca aacaagaacc tgatagctgt
      781 gaaaatacaa atcaattgaa tgaagctgta aagccccaaa ccggaaacga aaacaaacaa
      841 ccaaaaatac cgaagaaaaa atctaattat agcaagtata tattcgcatt gtttaccgca
      901 cttattctag taattgtcgt tactggcggc tatattgttt atacattaaa acagcaagaa
      961 gcagaagctc aagccaaata tgaaactgct gcaaaaaatc tcatggcttc aatccaagaa
     1021 gagcaagacc aaagtggaat ttcaacgaaa atagatacta taaatgacgg agaaaataag
     1081 tgccttgttt accgtccagt tgatgaaaat actgttcctt tcaaaaatgc aaaccagctc
     1141 ttagacgagc ttgctcaaaa gcaacaaaaa aagcatcgtg aaaaagaagt gctcacagtt
     1201 gccagaataa aagcaacagc aatatcttct aaagttaatc aatatagaat tgaagcagat
     1261 agttttatct gggatcgcag taaggaaaat ttcaaaaagc cagatagtat ttctgagaaa
     1321 gatatctata ttttcaaaaa aactggcaaa gaaatcacaa ataaggattt gtttccagat
     1381 gaaggaagtc tcttaggtat tcaacaggtg attcagcaat ccatacttga tcaagccaaa
     1441 gaacctgaaa aaatcattga tgctgtatta acaatggata gaatttctta tgatagtaaa
     1501 atgacttata cacctgatga attgactatc gtcttaccaa aaaattcaac tggaacttct
     1561 aaagtcactc taaaatacaa agatattgcg ccattcattg atacagatct ggtttcgcaa
     1621 gaaagtataa aagatacatt acctgcatta gatgaaaata aaaagtacgt cgctcttact
     1681 tttgatgatg gtccaaataa tagctcaaca cttgatttgt tgaatatact aaaaacagat
     1741 aacgtgaaag ctactttttt catgttagga cagatggtgg atcaaaatcc agatgttgca
     1801 aaacaagtcc acgacgaggg tcatgaggta gcatgtcact cgtattctca tccacaattg
     1861 aatacacttt cgacagatga attacaaagt gaaataaata aagctaataa agcaattttc
     1921 aaggctacag gcgtcttgcc tcgcaatatc cgtccccctt atggcgctat tgacaaaaaa
     1981 agtgctgaaa caattggcat gccaattatt caatgggata ttgactcaca ggattggaaa
     2041 tcaaaaaatc ctagagcaat caataatgtt gtcaaacaga atgtttataa tggtgcaatc
     2101 atcttaatcc acgatatcca tcctgaatca gttaaagcca taccaaatat gatctccatg
     2161 ttaaaaaata aagggtacga atttgtgacg atcgatcaac tattgtcagg aaaacaaaaa
     2221 ccattgcatc aatattttgg tatgaatgat gagcgactag tagactaact gtaattagat
     2281 tgagtggtat ttgaagaaat tatatttgta gtttccttat ttgatgaaaa gtcatgtaaa
     2341 acaaattaat aaagagtatt aagtatacta actgttgtaa gtatacttaa tactctttta
     2401 gttattaact ttagttagtc tcgttagcgt agcgtacgaa acaaaaaaac acgcatttct
     2461 gtgcgtggac gaaatagctt tagcgataaa aaactccttt cgatatacta ataatggcta
     2521 ctaaccgcat atcagatcga aaggagtttc taatgtttac cgacgataaa agtgtagcac
     2581 atacgagatg tcatattttc tttacccaaa gtatcgacga aaagtgattt atggaaagtt
     2641 aagagtaggt attggtcaga tattaagaaa attgtgtgag atgaaaaata ttgaaatcat
     2701 taaagctcat gttatattca tttgttagta agaataacgt caaaaattag tatttccagt
     2761 tttatgggtt actaaaaaga cgtcactgat caatttagaa aggtaaatat attctggtaa
     2821 taaagataga ctgaaccccg taaaaacctc attgcaacga atcaaggaaa gacgcttttg
     2881 cttctccctt gattgggcac attctttgat tttgattttt gatattgatt atatctttta
     2941 attcgccata gttatttata caataaagaa ctaaattttc taagaataga ctttctagat
     3001 tctttatttt cagcttttac taaaatgaag tcatcctatc cattttattc aaaacaaatt
     3061 ttttaataag aaagatctgt tctttttctt tgaaggaaaa gaaaaagaaa acgtgaaaag
     3121 aaacttatgc aatctatact gctaataacc tctataagat cgttgtatat tgatattatt
     3181 atcatgttta ttttaggaag aatactatga gttaagtttt taataagaaa tgtcgagttt
     3241 atgaaccttt tttcaaagtt gaaaaaattt ctttatttga taatggcgat caacatcttt
     3301 tagaatggat gcaatggtta aactgattgt tgagtatata atcacttgtt tgtttttatt
     3361 tatcttttcc ccactatatg gttaatagtc agccacttta gaaagagttt tagctgtttt
     3421 ttacttgagg aacatcattt tattggcagt cttatttgac ttattaagtg aatttatatt
     3481 aaaaaaattc gtttttactc ataataatct ctactaagat tcctccttta tttgagtatc
     3541 attctcattt gattataaat taaatccttc cctttattat tatcaacgag aaaaaaggtt
     3601 aaatgaatac aaaagaaata gatc
//
LOCUS       DAXLPI010000167         3588 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXLPI010000167 DAXLPI010000000
VERSION     DAXLPI010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.173"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(65..670)
                     /gene="ytpR"
                     /locus_tag="ACF3T5_002574"
     CDS             complement(65..670)
                     /gene="ytpR"
                     /locus_tag="ACF3T5_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFD3633092.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(875..1339)
                     /locus_tag="ACF3T5_002575"
     CDS             complement(875..1339)
                     /locus_tag="ACF3T5_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFD3633093.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(1361..1681)
                     /locus_tag="ACF3T5_002576"
     CDS             complement(1361..1681)
                     /locus_tag="ACF3T5_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFD3633094.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(1824..2903)
                     /gene="pepA"
                     /locus_tag="ACF3T5_002577"
     CDS             complement(1824..2903)
                     /gene="pepA"
                     /locus_tag="ACF3T5_002577"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFD3633095.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            3103..3453
                     /locus_tag="ACF3T5_002578"
     CDS             3103..3453
                     /locus_tag="ACF3T5_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFD3633096.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
BASE COUNT          987 a          765 c          651 g         1185 t
ORIGIN      
        1 tgagaagctg ttttttgaaa ttgctccttg tgactcagag tgaaacggca gactcacaac
       61 ctctttattt tggaaatgct tctccgacta cggcatcctc aggcaatact aagattcctt
      121 tttttgctgg agcatttggc aaatgcaatt cacgagcgga acagatcatt ccatagctat
      181 ctacccctcg caaagttcct ggccagatca tcattccatc tggcatcata gctcctggtt
      241 ttgcaacgac aactttttgt ccagcttcga tattgggtgc accgcaaacg atttgcaata
      301 ctgtaccatt gtctacttct gtctcagtaa tcgataaatg gtcagaatca ggatgtggga
      361 cgcatgtttt tacgtacccg acaacgatct tcggttcatg atcagcttca agctgttctt
      421 cgaatcctgc ttctttgatc aatcgattca atttcgctaa ttcctcatct gttaagctta
      481 cttggccgtt cccttcgatt gttagatgat cggagatatg gaaaaagttc caagcaacaa
      541 cttgaccttg ttcatctttg atccgagcaa cattccattt gcgttcaaca gtgttttctg
      601 ttcctttatc gtcggctata ataaccatca aggtgtctcc gacgtgtgct ttgttataag
      661 caaaaatcat gttctttcct ccattatcct ttgctgagat ttatcttaac aaaaaattgc
      721 attcactgca tgagattttt ttcatgcttt atttccgaag aagcctcttc tggaacacca
      781 tttattcgta acaagggact gtgacaagac ttttgtcaca gtccctttac tgttttagtt
      841 aattgattaa aaaggtcgta caagttccac ttgcttaatt acttggctgt tcttcttcgt
      901 cagaaacgat caagacatca caaggtgaat gacgtatcac gtagctggct acacttcctg
      961 tcataaagcg ttcgactgca tttagacctg actgcccaac catgattaga tcgatctcat
     1021 atttttttgg caatttgact gccatggatt cacgaacaga accatatgca ataattcctt
     1081 ctatatttgt gtaatcaaca gatttcccat attctttaca ttcttcgatc atttccttgg
     1141 ctgtttcagt ttgctgatcg actagatttt gatcaatagg tgcatagccc ataaagttat
     1201 aaaattgctg atcgattacg tttgctacat atactttccc attattacgg cgagccactt
     1261 ccaccgcttt tttgaaagca gcaagtgctt gttcactacc gtcgatacct actaaaatgt
     1321 tcttatattc ttgattcatc ttgacccctc ctaacagatg tcagcctttt gtcacacttt
     1381 ctaggaacga ttcgatttcc tgttttgttt tacgatcttt attcactaga cgacctaatt
     1441 cttttccgtc ttggatcacg acaaaacttg ggataccaaa aatgttccat tcagcggcta
     1501 catctatgta cttgtcacgg tctacttcaa taaattgcca atcagaaaag tcattttcaa
     1561 tttcaggcat ttgcggtttg ataaaacggc agtctccgca ccagccggca gtaaagaaaa
     1621 atacactctt tccagtttct acgtagcctg ctaattcttc taaactattt gggataatca
     1681 ttctttgtcc tctactttct gtttatgtat ttttcaatta aattatacac ctttctttcg
     1741 aactaacaaa cattgatcct ctttttataa aaaaaatctg gcaaaagcaa tcttttgcca
     1801 gattttatag tttcactatc gcattatttt ccgtatacga tcgtattcac tgttgattta
     1861 tccaaacctt tcagtgtttg tatcaacatt tctcgtgcag cttcaaaatc gcgaatactg
     1921 aacatcgttt gatgcgtatg gatataacgt cccactacgc caatcactgt acttgggatt
     1981 ccttcgtttt gtgtatgagc agcacctgca tctgtaccgc cttttgaaac aaagtattga
     2041 taagggatat tgtttgtttc agcgatatcc agcaagtact cgcgcaaacg aggaagcatg
     2101 atcaaacctg gatcttggat acgcatcaat gtaccttctc ctaaatgtcc gaatgtgcca
     2161 tttttcgtca ctgtatcatc tgctgcagag cagtctactg caaagaacag atctggcttg
     2221 aatttattga cagaagcttt agaaccacgc aagcctactt cttcctggac attcgcgcct
     2281 gcgatcaatg tatgccctaa tgtttcattt tgcaacgctt ctaaagcttc taataccatt
     2341 gtacagccat agcggttgtc ccatgatttg ctgatgatat tctttccgtt ggctgttttg
     2401 atcgtttcag cgtaaggaac gattgtgtct cctggaagga caccgtaact cattgcctct
     2461 tctcttgatt caaacccagc atcgaacaaa atatcggtta cttctactga tttttgtccg
     2521 cttgttcctc gcaataaatg cgggggaacg gaagaagaaa tacatggata attcttacca
     2581 gtagaggttt taagtgtaaa acgctgtgca gaaacaacgt aaggattcca gccgcctaga
     2641 ggtacgactt taaaaagccc gttttcttgg atttgtgtca gcatgaatcc gacttcatcc
     2701 atatgtgctg cgaccattac acggggtgca tccgcttctt catgatggcg cagaccaaaa
     2761 atgccgccca aaccatctaa ctgtaactca tctaccagcg gttccatatg actttccata
     2821 taagcgcgaa catcttgttc aaaaccgctt gttccttgta attctgttaa ctctttgatt
     2881 cgttgaaatg ttttttcttc catatgctat tcgtctaaat ttcagactaa actcctctca
     2941 gacttatatt gatctgcgga taaaatatga ttttttctgc agaccgatcg tatttattat
     3001 agcttatttt ttctggtagc ggttatttat ttcaaagaaa atatgagagt gtggtaaaat
     3061 aagcacaagg agtaacaagt gagaagaagg aggtatgtcc acatgtatga agaaagcgaa
     3121 ctaagctatt ttaaaggcgg gttagcagtt ggaatcggtc taggattatt gagcggggta
     3181 gcttcaacga tttggtatca tcgcaaaaaa gtattgtcag cagaccaagt acttgaaaat
     3241 atcaagtcat cttttctaaa agaaggtgca atcgaaggtt cttggatctc ttttgaaaag
     3301 gaacctatta ggaaattcgc cgttcggtcg agtgcttatc ggggaggtct ttctcgaata
     3361 gaagatggcg aaactgttta ttatgaattt ttagcagatg ctcatacagg aaccgtatta
     3421 gaaatttcaa gaaaaaaagg tgcaacagat taatacgtca aaaaagtgag agaacgaata
     3481 cgcacccact attcaaaaga gaggtatcct atacttcttt tatagtgggt aatttttata
     3541 aaaatagggc tatttcctct tggaatagct agtattatag ttattata
//
LOCUS       DAXLPI010000168         3552 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXLPI010000168 DAXLPI010000000
VERSION     DAXLPI010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.174"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            111..998
                     /gene="cdaA"
                     /locus_tag="ACF3T5_002579"
     CDS             111..998
                     /gene="cdaA"
                     /locus_tag="ACF3T5_002579"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFD3633097.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            995..2164
                     /locus_tag="ACF3T5_002580"
     CDS             995..2164
                     /locus_tag="ACF3T5_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFD3633098.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            2188..3543
                     /gene="glmM"
                     /locus_tag="ACF3T5_002581"
     CDS             2188..3543
                     /gene="glmM"
                     /locus_tag="ACF3T5_002581"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFD3633099.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         1142 a          652 c          842 g          916 t
ORIGIN      
        1 ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc atatcagcct ctaaaattaa
       61 aacatatgat atactagaga ggaaatctca aaagagaaga gaggcttttt atgtcatttc
      121 aaatcgggga actttttaat ttggactatt ggcagcaaat gttttccggt gacttgttct
      181 ctctgaattt tttggtgaac atacttgata ttctggttgt ctggtatctt gtatacaaac
      241 tgatccaact gctgcgaggc acaaaagcga ttcagcttct gaaaggtgta gctattttta
      301 ttgttatccg aatcgtagcg gaaatcattg gtctgcacac cttgtcttgg ctgatgaacc
      361 aagtcattac gtacggggtg atagctgcta tcgtgatttt tcagccggaa atccggagag
      421 gactggaaca tcttggacgc agttcgcttt tcaaacaatc aaaaagcgaa cagcaggaag
      481 atgagaagat gatcttgtct tttgacaaag ccatccaata tatgtccaag cgaaaaatcg
      541 gtgcgttgat cactatcgaa cgtcatactg gattagatga gtatattgag acagggattg
      601 ctttagatgc agatatcact ggggagctgc tgatcaatat ctttattcct aatacaccgc
      661 tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc agtagcaagc gcctatcttc
      721 ctctatcaga aagtatgttg atccctaaag aattcgggac tcgacaccgg gcagctgtcg
      781 gtataagcga agtcagtgac gcgattacca tcgttgtctc tgaagaaacc ggggacgtca
      841 gcatcacgtt agataatgaa ctgatggctg gcttatccca gcaagaatat ttggcaatct
      901 tgcgtagaga actaattgct gaagagaaaa caaaaaacaa gaaaaacgtg attcaatctt
      961 tcctagacgg tatgactaag ggaggggatc gcaaatgatc tcaaaagaga aacggacaag
     1021 cattctttac agttttattg cattgctgtt cagtattgcg ctgtacttca atgcaaatgg
     1081 ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat aatcaaacag ttacgggggt
     1141 gccgatcaag atttcttatg attcaaagcg ctactatatc catggatatg aaaatgcagt
     1201 gactgtcaaa ctttccagtg caaaccgagt tcagttgaat gcagaggcaa acgaagatac
     1261 acggatgttc cgcgtcacag cagatatcac taagctgggc gaaggaacac atgaagttcc
     1321 gctaaaaatt caaaatttga gcagtgctgt cacagcaaag atcgaaccat cgactattac
     1381 agtaacggtt gagaaaaagg taataaaaga tttcgatgtg gaaacgttga ttccttcaac
     1441 gttcacacca tctggatatg aacttgatga tacttccgtt agtcctaaaa aagtctctat
     1501 cactacaggt gacaaaacat tggcagaaat caaacgagta gtagctgatg tggacgcttc
     1561 gatggtcaca gatgatggga ttaatagtga ggtcccaata caggcgctga atgcagcagg
     1621 ggaagtatta agtatcgtct ctgatccagt gcaagtaaca gtgagagctg attcgatcaa
     1681 gccaagcaag tcggtacgac tttatgggat acagcaagga acaccagctg ccggggtaga
     1741 aagctatgat ttcagttttt caaaactgga agcagaagta agcggctcta gcgatttgct
     1801 tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc tcaggtatca atcatcgaac
     1861 aactcgtaaa attgaaatac cagtagaaga ggggttaagt atcaagcccg aatcagttag
     1921 tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc tctgcttcga cgagcagtcg
     1981 gacacaagaa aatacaagta cgagtagaag tatttcatct gctgattcag gaacggaacc
     2041 atcgacaaca accatttctt ccagttcgga atctgggaca agcagctcga ccacaagtac
     2101 agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc agaacaaatt cctcagaaaa
     2161 ttaagttaaa ggagtaattt tacaactatg ggtaaatatt ttggaacaga cggcgtacgc
     2221 ggtgaagcca acaaggaact tacgccagaa cttgcattta agctgggacg gtgcggaggt
     2281 tatgtcctaa gccagcatga agaagaagga aaacgcccac gcgtgttagt agggcgggat
     2341 acaagaatct caggtcaaat gttagaagaa gcgctgattt caggactttt atcggtgggt
     2401 atcgaagtgt tccagctagg tgtgatctct acaccaggtg ttgcttatct gacacgtttg
     2461 caaaaagcaa gcgcaggggt catgatttct gcttctcata atccagcaca agataatgga
     2521 atcaaatttt tcggtgcaga tggttttaaa ctagtagatg accaagagct agaaattgaa
     2581 gcattattag atgctgaagt ggatgaattg cctcgtccat cagctgaagg attgggaact
     2641 gtagaagaat ttccagaagg cttattgaaa tattctcaat tccttgtaca aacgatcaac
     2701 ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa acggagcaac ttcaacaagt
     2761 gttaatcgtt tgtttgccga tcttgaaaca gatttttata caatgggcac aaatccagac
     2821 gggttaaata tcaatgacgg cgtaggctct acccatccag aacgtttagc tgagatggtc
     2881 gttgaaaaag gagcagatgc tggactagct ttcgatggcg atggcgatcg tattattgcg
     2941 gtggatgaac ttggaaacat tgtcgacggt gataaaatca tgtttatctg tgctaaatac
     3001 ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta cgactgtcat gagtaactta
     3061 ggattccaca aagcggtgga aggaatcggt ttgaaagatg tcgttacaca agttggtgat
     3121 cgttacgttg tagaagaaat gcgcaaaaac gattataact ttggcggcga gcagtctggg
     3181 cacatgattt tcttagattt taacacaact ggagatggga tgctttcagg aattcagtta
     3241 ctgaatatca tgaaacagac aggtaagaaa ttatcagagc ttgcagcaga ggtaacaatc
     3301 tatccgcaaa aattagtgaa tatccgcgta agtgataaaa acggtgcgat ggatgttccg
     3361 gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg gatcagaagg aagaatccta
     3421 gttcgtcctt ccggtacaga accattactt cgagtgatgg cagaagcgcc tactgatgag
     3481 aaagttaatt actatgtcga taaaatcgcg gcagtagtca aagaagaaat cggactggat
     3541 taagagatat tt
//
LOCUS       DAXLPI010000169         3524 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXLPI010000169 DAXLPI010000000
VERSION     DAXLPI010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.175"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..324
                     /locus_tag="ACF3T5_002582"
     CDS             <1..324
                     /locus_tag="ACF3T5_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFD3633100.1"
                     /translation="IDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGLHDQLEEKNE
                     ENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMIRKRLLLEAE
                     KAVKE"
     gene            321..2640
                     /locus_tag="ACF3T5_002583"
                     /pseudo
     CDS             321..2640
                     /locus_tag="ACF3T5_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
     gene            2701..3243
                     /locus_tag="ACF3T5_002584"
     CDS             2701..3243
                     /locus_tag="ACF3T5_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633101.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            3321..>3524
                     /locus_tag="ACF3T5_002585"
     CDS             3321..>3524
                     /locus_tag="ACF3T5_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633102.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWR"
BASE COUNT         1294 a          512 c          703 g         1015 t
ORIGIN      
        1 atagacatac attgtacaca tgcggacttg gatcttaagg ctttacaaat tgattatgat
       61 tatttcctac cagatgaata tgctagaaat ctctatcatg gactgcatga tcaattagaa
      121 gaaaaaaacg aagaaaatat gttttataaa atcgttcaat cttttgagaa tgagacggct
      181 aaaaaattgt taattcaatt ttacgaagaa caagcaacgg atcgtcttca agaatatgtt
      241 gaaaagttga tcaaaaatga agaaatttct gaaatgatta ggaaacggct gttattggaa
      301 gctgaaaagg cggtgaaaga atgatcattg gaggaatttt ctctggcata gccgactggt
      361 ggtcaggttt attcggagaa ggggatgcag atcccaaaac cgtaacgaaa gtcttacaag
      421 attatggtaa ttatctgcag tatcaagatg tattcagttg gatttgtaat acttttttat
      481 ggggattgat caaagggtta tataatctca atactttatt agaaaaaaca atttatcaaa
      541 gctttgattt aaaagactta ttgaacgccg ctggtgttaa tgaactattt caaagtttga
      601 ttagtaaggt attggctctt ttttgtgtcg tcacattaat ttatttaggc ttgaaatttt
      661 ttatgagtaa acacccaccg aaagtcaaaa atatcttggt taatttattt atggccatga
      721 tcttgatttt tggtggtagt tctttaatcg atgaagggtt aaatatcagt aaaaactttt
      781 atggagacgt cactgcagca aataaagcag ataaaacggg ttcacctgct tttcaaatta
      841 ttcaaaataa tgtgattgat gtcagtacat tactagcgac tgacccgaat aaagtagaat
      901 ccttaccaac agataaaaga aatgctttaa cagcgaaaaa tttcaaaaca gcgaatatca
      961 atgggattat tacacctgat caagctaaga aaatgtctga atcaagtgat attagtacag
     1021 tacaaaaaga tcggttaaag gcattacagt atcgactaga gttagacgat aatgggaaag
     1081 aaatccctgt tcaaattagt gatgaaggtt tagcaaaata tgtgtatacg tcaggctatc
     1141 gtcgatacat tagtagtcca gggattattt tggctgggga atcttctttg tcggtagcct
     1201 atttatttat tttgtttaca atcgttactt gcattattga gctagtattt aaaaaattct
     1261 atttagtaat tgccgcgtca acggactttg agacaggaca acggatgaaa agtgcgatag
     1321 aagatatttc tcaatccttc ttgttattgg cgtttacgat tttagaatta cgaatctata
     1381 cattaatgct ttcaggtatc ggtgatttgc atgcggtagg aaagatcaat ggattcttgt
     1441 atgttgttgc tttaattgtg ctaactattg cgctattcaa aggctctcaa tcggttacga
     1501 agattttcgg tgttgatacg agtctgaaaa acggcagcaa ttctcttctt agcatgtttg
     1561 ccttaggaaa tattgcgaaa aatgtgggta gcggagctaa aaaccttgca agtgctggta
     1621 agagtggttt aagccattta aatgatattc gtaagaatgg atttggccat cgaaaagata
     1681 ttacccatga taacggggaa gaacaatcag acgataaagc tacaactgta cctaatagta
     1741 agaagacagt ttccgaaatg ttcgggaaag cgaaaaatgg tgtttctaaa gccgcagaag
     1801 gtcttggtta cgtcaatgaa cgtgggttaa aaggaacagt tgaagatctt acagaaaaag
     1861 ccaaagacac cttagaagag aaattagacg gcacgagagc taaagaattt gctgaagctg
     1921 tgaagaatcc atccgaagca ctagaaaaac aaaaagaaaa agcttcagca gtcaaagaag
     1981 gactaaaaga tgcgtatggt gaaggacaag tcaatgcagc agtgaaggca aatacaacaa
     2041 gtgaaaaaga aaatcaagaa ggtgcagcga ttccttcagc caaagatcac caagatgagt
     2101 tacattctaa cgaaaattcg atcggagaaa aagattcaga agaaggacaa catggtcagt
     2161 taccaaaaac cgatccagaa caatatcgtt ataaatctaa cattaattct gaaaacgaaa
     2221 aacgcatttc agatttacca aaacgaaatc aagaagtaca aacggatgag gattccatcg
     2281 atctgccaaa tgataatggc atcgaagttg gaactgaaaa atcaacgaaa attggtttgc
     2341 ctaagacaca aaatttacca atgaaagggt actgttcaaa tgaaatcaag tcgtttgcct
     2401 gaaaatggaa atctgacaac tgtttcttac aatttagctg aaagtggtga aacagagaat
     2461 gcgtccgttc aaaatattgt gacaaaagaa gctgcaccta agccaatcaa taaagttcat
     2521 aatggataca gaccagcaaa cgtagcattt actgcaccga caaaaataaa tgtgacaaga
     2581 gaaactaaaa acaggaacaa tgaattgaaa gaacaactat tgaaggatat caaaccataa
     2641 aaggcctatt taagggcctt tttctttata aaacaagttt aaaatggaaa ggagaaaatt
     2701 gtggaggata ttttaatagc tacgtttata actgcactgg gagcattttt aacgatattt
     2761 gtggtgacta ttaaaggatt taaagatacg gatagtttac aaaaagatca tcagtctatt
     2821 ttagatggtc aaaaaacaca taccgaaagc attattgtac acctttcaga aggattcact
     2881 tatttgaaag atgctatgcc cacattcaca gtaaaattaa cggaaatatt tcattctgtt
     2941 gagcaaatat cgtcagaatt gaaagagcaa agacaacaaa aagatcgtca acttggctca
     3001 cttaatttta ggcaagaaac gatacaagaa tcattggaga atgtaaatcg attaaacgat
     3061 gaaatgcaac gagttcattt ggaaaatgaa gaattacgcg cacaaaatta tgatttaatt
     3121 caaaagaaaa gaaatctgga acaaaaaatt gttgaattaa aagaagaaaa tcgtcaattg
     3181 aattatcaat tacgacgagg aaaaaatcgt tctcgtgatt taggaaatga attagagttg
     3241 taatatttag aaaaacgctg tgagaaatca cagtgttttt tatttctaaa aatacaaaat
     3301 ataaggggta tggttatatt atggataaat acattcatat gaaaaatcct ccaagattta
     3361 gtggggaatg gaagtttttc gcctttttct ttttagatct attaacgctt ggtggctgct
     3421 ttttttgggc gaaaatagtc aataatcttg tccacttacc actctttttt gcactcttga
     3481 attatggatt atggcttgcg atagcgttag tgattgtttg gcga
//
LOCUS       DAXLPI010000170         3517 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXLPI010000170 DAXLPI010000000
VERSION     DAXLPI010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.176"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..575
                     /locus_tag="ACF3T5_002586"
     CDS             <1..575
                     /locus_tag="ACF3T5_002586"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3633103.1"
                     /translation="ENLPYYNKTTSSKKFITACRLDKQKNLPLMIDAIGKLIHEGYEC
                     ELDIYGEGGLKTELESYINHNGLTEFVHLKEFTNKIHEKMQNAAGFVISSDYEGISNS
                     MLEALAIGVPVIATDCPVGGARMYLGDRNEIGHLVECNNLDKLTDAMRRVLLDRESVV
                     RKAQKGIKLRKELTSKNISNIWYEYLNQFF"
     gene            594..2018
                     /gene="wzy"
                     /locus_tag="ACF3T5_002587"
     CDS             594..2018
                     /gene="wzy"
                     /locus_tag="ACF3T5_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy"
                     /protein_id="HFD3633104.1"
                     /translation="MKEKINILMFSLTLVAFTIFCAFSLNQNYWLNWLGILIYLYAIC
                     TWHWRKGDSIFSLYTIFFTLFLLFSYGQCIMWAFGIGTDRGIGTTVVAYGTGIIPSES
                     DMIMVKWYSCISMFVFHIGALLFTRKTTLRKVSWYESGDADADRKFLFKIGCIISIIV
                     VPIVFVSKLLEVRIALVHGYNALYYGDYATQSGYLQIILYLFFPALICLLIGSNFSKV
                     ITRFVFIIFALYSLLGIMSGDRGSWLYSSIILIWAYTQYKGTFNYKKLIKWLILAVIG
                     IYILNVITKARDGGGLSKLTLKDFTSVFDSDDSPLVDSFFEMGGTMSIITFFLISGNG
                     IYPFANTYLTSLLGSISTRMLSMFGIKFVLLADWFSQDYLGITWGTGFSMIAEAYVNG
                     GYIGGIVYIFIIGIVIGKILSNSIDNQDIENHPMKMFLAITTANIIMGFPRAASYLIV
                     KNFVYGVLIILLINFFLQQFNKRL"
     gene            2064..>3517
                     /locus_tag="ACF3T5_002588"
     CDS             2064..>3517
                     /locus_tag="ACF3T5_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFD3633105.1"
                     /translation="MERTARLKKNTIWALVFQITSIVCGMILLRLILTKYGSEVNGMI
                     SSINQFLQIISFLQLGVGAVMQSTLYKPLAEKDNLMISQILASGGKFYRQIAYILLVY
                     VGLLLAVYYVLVPNEFGRSYTMAMIATLSINSFAQYYFGIVNTLLLSADQKIYITYIA
                     QTIAFISNTVACALLISLNTEILMVKLITAIIYLACPIYYRWYVDRNYNIKRNVSYDK
                     EPIPQKWNGVAQHVASVVLDSTDVVVLSIFSNLIYVSIYNVYNLVIYGVKQLFMAMTN
                     GVQALLGELWARKEIEVLYKTFAWFEWIVHTLTVFIFGCTGILIVPFIQVYTRGVTDA
                     NYNQLSFAALLVTANALHCLRTPYHVMIKAAGKYKETQNSFIIASLLNLGLSLILVKP
                     LGLVGVALGSLISITYHTLWSANYNSKYLLKWPMKKFYKQLFVNILTVVLSFGATSQL
                     KMMSITYLDWIVLAIKCAVIVFICIILVNVLFYR"
BASE COUNT         1170 a          448 c          636 g         1263 t
ORIGIN      
        1 aggaaaattt accttattat aataaaacta caagtagtaa aaaatttatt actgcatgta
       61 gattggataa acaaaaaaat ttgcccttaa tgattgatgc aataggaaaa cttattcatg
      121 aaggatatga gtgtgaactg gatatttatg gagaaggggg attaaaaacc gagcttgaaa
      181 gttatatcaa ccataatgga ttaacagaat ttgttcatct aaaagaattt acaaataaaa
      241 tacatgaaaa gatgcaaaat gcggcaggat ttgtaatttc ttctgattat gaaggtatat
      301 ctaattctat gttagaagca ctggctattg gagtgccagt tatcgctaca gattgtccag
      361 taggtggcgc aagaatgtat ttaggtgata gaaatgaaat aggtcatctt gttgaatgta
      421 ataatctaga taagttaaca gatgcaatgc gtagggtact tttggataga gaaagtgtgg
      481 taagaaaagc gcaaaaagga attaaactaa gaaaagaact gacatctaaa aatatttcaa
      541 atatatggta cgaatattta aaccagttct tttaaccaaa aggaggatat tgtgtgaaag
      601 aaaaaataaa tatattgatg ttttcgttaa cgttagtcgc gtttacaata ttttgcgctt
      661 tttcattaaa tcagaactat tggctaaatt ggcttggaat attgatttac ttatatgcaa
      721 tctgtacatg gcattggaga aaaggagatt caatattttc attatacacg atttttttca
      781 ctcttttttt attatttagt tatgggcaat gcattatgtg ggcattcggt attggaacag
      841 atcgtggaat cggtacaaca gtagtagctt atggtacggg gattattcct agtgaatcag
      901 atatgataat ggtaaaatgg tattcttgta tttctatgtt tgtatttcac ataggagcac
      961 tcttatttac gagaaaaacc acactaagaa aagttagttg gtatgaatct ggagatgctg
     1021 atgccgatag aaagttttta tttaaaatag ggtgtatcat ttctataata gtagtaccga
     1081 tagtttttgt ttctaaatta ctagaggtac gtatagcatt agtccatggt tataatgcat
     1141 tatattatgg tgattatgct acacaaagtg gatatttaca aattattcta tatctttttt
     1201 ttccagcctt aatatgttta ttaattggta gcaacttttc aaaagtaata acgagatttg
     1261 tttttattat ttttgcttta tattctttat taggaattat gtcaggagac agaggaagtt
     1321 ggttgtatag ctctattata ctcatatggg catacactca atataaagga acctttaatt
     1381 ataaaaaatt aataaagtgg cttattttag ctgttatagg aatatatatt ctaaacgtaa
     1441 taacgaaagc acgtgatggt ggaggactaa gtaaacttac tttaaaagat tttacatctg
     1501 tatttgattc agatgattct cctttagttg attctttttt tgaaatgggg gggactatga
     1561 gtattataac ttttttccta ataagtggta atggaatata tccctttgct aatacttatt
     1621 tgacctcact cttaggctct atatcaacaa gaatgctatc aatgtttgga ataaagtttg
     1681 ttttattagc tgattggttt tctcaggatt atttaggaat cacttggggg actggattct
     1741 caatgattgc agaagcctat gtcaatggtg gatacattgg gggtatagtc tatattttta
     1801 ttattggtat agtcatcggt aaaatccttt ctaatagtat agacaaccaa gatattgaaa
     1861 atcatcctat gaagatgttt ttggctataa ctacagcaaa tattataatg ggttttccta
     1921 gagctgcttc atatttaatt gtgaaaaatt ttgtgtatgg tgttttaatt atattgttga
     1981 taaacttttt cctacagcaa tttaataaac gtctttaata tttaagctta ataaaaagat
     2041 aaaacaagtc aggagttgca aaaatggaaa gaactgctcg gctcaaaaaa aacactattt
     2101 gggctctggt gtttcaaata acttctattg tctgtggtat gatcttgcta cgtttgatat
     2161 taacaaagta cggatccgag gttaatggaa tgattagctc tattaaccaa tttctacaaa
     2221 ttatttcttt tttacaatta ggagtaggcg ctgtaatgca atcaactcta tacaaaccac
     2281 tagcagaaaa ggataattta atgataagtc aaatcttagc ttctggaggg aagttttatc
     2341 ggcaaattgc atatattcta ctagtatacg tgggtttgtt acttgcagtc tattatgttc
     2401 tcgttcctaa tgaatttggg agatcatata caatggcaat gattgcaaca ttaagtatta
     2461 attcatttgc tcaatattat tttggtattg ttaatacttt gttattaagt gctgaccaga
     2521 aaatttatat tacgtatatt gcacagacaa tagcatttat ttctaataca gttgcatgtg
     2581 ctttattaat tagtctgaac acagagatcc ttatggtaaa acttattact gctataattt
     2641 atcttgcttg tccaatatat tatagatggt atgtggatcg aaactataat ataaagagga
     2701 atgtgtcata tgataaggaa ccgatccctc aaaaatggaa tggggttgca caacacgtag
     2761 cttctgttgt actagatagt actgatgttg tagtgttatc tatattttct aacctgatat
     2821 atgtatctat atataatgtg tacaatttag taatatacgg agtaaaacag ctattcatgg
     2881 ctatgacaaa tggcgttcag gccttactag gggaactttg ggccagaaaa gaaatagaag
     2941 ttctttacaa aacatttgca tggttcgaat ggattgtaca tactttgaca gtttttattt
     3001 ttggctgtac cggtatacta atcgttccat ttattcaagt atatacaaga ggagtaacag
     3061 atgctaatta taatcaatta tcattcgcag ctttacttgt aactgctaac gcacttcact
     3121 gtctgcgtac accttatcat gtaatgataa aagcagctgg taaatataaa gaaactcaaa
     3181 atagttttat aattgcttct ttgttaaatt taggtttatc attaattttg gttaaaccac
     3241 tagggctagt tggagtagca ttaggatctc taatttcaat cacatatcat acactttgga
     3301 gtgcaaatta taattcaaaa tatttgctta aatggccgat gaaaaaattc tataaacagt
     3361 tatttgtaaa catcttaact gtagttcttt cttttggggc gacatcacaa ttgaaaatga
     3421 tgtcaatcac atatttggat tggattgttc tcgcaattaa atgtgcagtt attgtattta
     3481 tatgtataat cctagttaat gttttatttt atagaag
//
LOCUS       DAXLPI010000171         3510 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXLPI010000171 DAXLPI010000000
VERSION     DAXLPI010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.177"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..2232)
                     /locus_tag="ACF3T5_002589"
     CDS             complement(<1..2232)
                     /locus_tag="ACF3T5_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFD3633106.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTSNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="ACF3T5_002590"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="ACF3T5_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          994 a          776 c          549 g         1191 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtattgcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagttttact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac aacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata
//
LOCUS       DAXLPI010000172         3453 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXLPI010000172 DAXLPI010000000
VERSION     DAXLPI010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.178"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            674..1900
                     /locus_tag="ACF3T5_002591"
     CDS             674..1900
                     /locus_tag="ACF3T5_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3633107.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2058..2675)
                     /locus_tag="ACF3T5_002592"
     CDS             complement(2058..2675)
                     /locus_tag="ACF3T5_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3633108.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            2939..3319
                     /locus_tag="ACF3T5_002593"
                     /pseudo
     CDS             2939..3319
                     /locus_tag="ACF3T5_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3319..3453
                     /locus_tag="ACF3T5_002594"
                     /pseudo
     CDS             3319..3453
                     /locus_tag="ACF3T5_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase"
BASE COUNT         1228 a          559 c          546 g         1120 t
ORIGIN      
        1 attttaaatt tttacatacc tgtttatgaa aaactaaata atctgtcaaa aaaatatgat
       61 tgttcatttc taaatatgtt ttccaatttt aaaatctttt ttagcatttt tgtttccttt
      121 tcatatggtt tttcgttgtt aatagtgact aggatgatat tcctattttg accattgaca
      181 attaaatata atttttgaat aacgttccct gctattcgag ctaagaattc aagtacgcca
      241 tgacctattt agcgataact acttagagat ttctttttgt tttgggaaga caaaatgcca
      301 tgacaatagc agtagataat gatacttccg taaggctcgc cgtaaactgg ggagaggtga
      361 aattcctatg tgcttaccca agtcaattca aaaattttag ttcatttata aggggatgat
      421 tttattaaat caatagtcta tctaaacaaa aaacaaacct gtgagttttt aggaattgat
      481 aacaatactt tagataaatg gattcgtata tataattttc ccgtaataaa aatagacggt
      541 ataattcgct ttgatgtaga agatatcaaa ggctttatgg atacgttcaa aaagatttga
      601 attttacttt tgactgcata tactaaaagt agcgaaatat actgccttac ttttttatta
      661 aggagtgagg aagttgccta cgaagctttc aaatggaaaa tataaaacta atttgagata
      721 tccaaaaaaa tttaaagaaa ttacaggtat cagttcagaa aaattccaaa aaacatttcc
      781 aactaggcaa ttggctatta aagccgaaaa tgaaatgaaa aagaaaatcg caaaagtctt
      841 aagagaggag aatgcaaatt cattagagct caaaggtcaa ataaaattca aggagtttta
      901 taaatctaaa tggcttccta gatatgagtt agggcaaact actagatccc ataaagctcc
      961 aagcgatata accatcatta atacaaagaa tttatttcga ttgcatattc tgcctatatt
     1021 tggtgagtat gccatgagct atcttaatgc aaataccgaa ataatatcag atgaactcac
     1081 aaaaaagtcg aaggaatatg caaacataaa aatcattaaa tgctatgttc gttccatgtt
     1141 tgatattgca gaggttctaa attatattga atttaatcgt acaacaaaaa taattcaaag
     1201 tattactgca cctaagaaag ttgctctgga agaaaaaaga atacgagaag gaaaacaagc
     1261 tttatcttcg aaagaactca ctgattggtt agacgctgta agagatgatt tcaatgatca
     1321 attactttcc tttcacgatt acactctttt tatgcttact ttatatcttg gggatagaaa
     1381 gagtgagacc tatgctttgc aatggaaata cattgatttt gaaaaccaaa cagtccgtct
     1441 taaatatgcc ttggataaac acacaaaaaa gaaatttaca aaaggtcgaa aagatactat
     1501 tatccaagtt ccggaaattg taatgacact attgcaagaa tggaaatcag tgcaagctga
     1561 acaactattg aagttgaaaa ttaaacagac acctgaccaa tatcttttta gttactccaa
     1621 accttctggt gaagtaaatt gtccagtcca tactgattac ttaaattatc gaattaattc
     1681 tataaaaaaa agacatcccc atttggttca tttaactcct cataaacttc gacatactta
     1741 cgcaacttta gctagacaag gtggggctga tatgaatcaa atttctaacg ctttgacaca
     1801 ttcaggtatt tcaactacaa aaatttatgt gaatacacct gatttagttg ataaagctgt
     1861 gttcgaagcg tttcagcgag gattacaaaa atctaattaa tgtgttctag aatgtgttct
     1921 aaatataaaa aaaagacact ttccaaataa ttggaaagcg tcttgaacgt tgatatataa
     1981 aggatattaa cgttttgaga attgagatgc tttacgagct ttcttaagac ctggtttctt
     2041 gcaaaattat attagcctca actgaaagat ttattttttt gctccctaac ttcattagta
     2101 ataccatgtt tcattcgata tcttctaaac gttctacgat taatccctgt tagctcttca
     2161 acttctttat ctgtagatcc agtcaaaaac aaattaaatg ctaattgaag tcgcggatca
     2221 ttctcacgat attttagcgt acctcccttg tatttccctt ttgctttagc gagtgcaatt
     2281 ccttgttgtt gcgtttcgcg aatataccga cgttcttctt cggcctggta cttgtatagt
     2341 tcgataatta agttattaag taatcgacgt aaattatcat cagtgatacc tgttaacgct
     2401 ggcaaattca aaacttccag tgtagctttc ttcagctgaa ttaaattcat cgtttctgtt
     2461 agctctttat tatttcgtcc aagtcgtttt aattctgtca caacgacaat atcaccttca
     2521 cgtatgaatt tcatcatttc ttgaaatcct tctcgattgg tatctttccc acttgcttta
     2581 tcactaaaaa ttttatcaca ctctgataat aattctaatt gtcggtctaa attttgatct
     2641 aagctgctta ctcgagcata cccaattttc gccataataa aactccttta aaattctgaa
     2701 atccggatca tttttaaatt ctgttgccca actctgttgt catttttcta gataaatggc
     2761 ataaattttc agcgctaagt gacaaaagca actaattttt catgatagat ttttaaatat
     2821 ctcaatctat ctccaaaatt agttgctttt gtagctatag ttgactatga atatgacaaa
     2881 taattagaaa aaactgtgcc aattaaatat caaaatgaca aattgatgaa tcaaattcaa
     2941 attaaggggg gcaaccctgg aagtcttaaa tttaccctca atgaatggta ttgaagatga
     3001 aaatttaagg cgtttgatta atagccttgt cattgaattg tacaagtatc aagcagaatc
     3061 agaacgaaaa aaaattaagg aacgtcaggc acaaggaatc gaaattgcta agaaaaaagg
     3121 caaattcaaa ggtcggcaac tgaaatttaa aaaaaatgat ccacgtttaa agcatgcttt
     3181 cgatttgttt ttgaatggtt tatccgataa agaagttgaa gaacaaactg ggatcaatcg
     3241 ccgaacgttt agaaggtatc gagcaagata taacgtgaca gtagatcaaa gaaaaaacaa
     3301 tgaaaagagg gatagttaat gagtacggtt attttagctg aaaaacaagc caggcattag
     3361 cctacgcaag tgctttaaaa caaagcacca aaaaagacgg ttattttgag atcaaagacc
     3421 cactatttac agatgaaacg tttatcacct ttg
//
LOCUS       DAXLPI010000173         3421 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXLPI010000173 DAXLPI010000000
VERSION     DAXLPI010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.179"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            82..1821
                     /gene="yfcC"
                     /locus_tag="ACF3T5_002595"
     CDS             82..1821
                     /gene="yfcC"
                     /locus_tag="ACF3T5_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFD3633109.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1978..3162
                     /gene="iadA"
                     /locus_tag="ACF3T5_002596"
     CDS             1978..3162
                     /gene="iadA"
                     /locus_tag="ACF3T5_002596"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFD3633110.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3273..>3421
                     /locus_tag="ACF3T5_002597"
     CDS             3273..>3421
                     /locus_tag="ACF3T5_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFD3633111.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKAR"
BASE COUNT         1067 a          576 c          805 g          973 t
ORIGIN      
        1 tgtcattttt actgtataat tcatttgatt atccattttt tattcatcaa aattactgaa
       61 aaaagaaagg aagaaataaa tttgaagaaa acaaattctt caggaaaacc gaaacgcgac
      121 ttcactaaat tatcgacacc ccatacgtat gtcattattt ttggtgtagt catctttgca
      181 tggatactga cttttgtcgt tccagcagga aagttcagca cgcaagatat tgaatacaaa
      241 gatgcaaatg gcgaaacaag tactcgtact gttttacgtc aagattcctt ccgttatgct
      301 tacgagctgg ataagtcgta tgtgtttgat caattagaag aactgcaaga tcatccagca
      361 gaacgtgaaa aactggatgt tccggaaaaa ggattggaaa aagtcattgc tgacggagaa
      421 aaaaatctta cgcaggaaaa actagatgaa atctccttga cagatgatgt gctttacgat
      481 cagtacggcg aaaatatcta tgatacttct aaaaaattgc ataaaacagc caaaatatgg
      541 ggaacagatg attttggcgg ctttggtttc ttaaattttg tttttgaagg actagtatct
      601 ggagataagt atggatctgc tgtagggatc gctgcattga tccttgttgt tggaggtgct
      661 tttggtatta ttatgcgtac cggagcgatc gacgcaggta tctatgcgtt tatcagtaaa
      721 actaagggac tggagcgttt agctttaccg ctattattct ttgctttttc attcggtggc
      781 gctacattcg ggatggcaga agaggtaatt ccattctcaa tggtgatggt tccatttgtc
      841 attgcgttag gatatgactc gatcgtagca gtaaccgtga cttatgtcgc atctcaagtc
      901 ggaaatgcga cctcttggat gagcccgttt agcgtagcgg tggctcaagg gattgctgga
      961 atccccgtgt tgtctggtgc gacattccga ttgattatgt gggtagttgt tactgcatta
     1021 gccgctggtt atatgatgat atatgcagaa aaaatccgta aaaaaccaga aagttcgttg
     1081 acttacaaat cagatgatta tttccgttct catatcaaaa agacatctga tgaaaataaa
     1141 aaattcatgc ttgggcataa attgatctta ttagagatgt tagttgtcct tgtctggatc
     1201 gtttggggcg tgactcaaaa aggctattac attccagaaa tcgcttctca attctttgtg
     1261 atgggattag ttgctggaat cattgctgtt cttttcaaat tagatggaat gaaagtcaat
     1321 gacatcgctt cttctttcca atctggtgca gctgatttag ctggtacagc aatcgtagta
     1381 ggtatggcca aagggattct tcttgtcttg ggaggatctg atgcatctgt tccttcagct
     1441 ttgaatacga ttttgcatgg gatcgggacg gcgttgaccg gtgttccagc tgtcattggt
     1501 gcttgggcga tgtacatctt ccaaagtttg ttcaacttgg ttgttacatc aaattcagga
     1561 caagcagctt tgactatgcc aatcatggct ccactggcag atttagtagg tgtatccaga
     1621 caagtagcag ttcttgctta tcagcttggt tcaggtttta tggacgcttt tacacccgta
     1681 tcagccagct tgattggtgt gctaggtgtt gcacggatcg aatgggcaaa atgggcaaga
     1741 ttccaaatca agatgcaagg attcctgttt gttttaggaa ctattttcat catgattgcc
     1801 attgcaatag gtctgcagta gtactggaga cctgtaaata aaaaagtaat ccaaacagta
     1861 gataaggact ccaagaaccg tcattttacg gtaaaatcaa aggagtaaag atcctatcgt
     1921 ttggattatt ttttaagatc ctatcatcag ttatacttaa agaaagagga aaaatgtatg
     1981 aaaatcattc gacaaattga agtgtttgca ccagattact taggaaaaat ggatgtactg
     2041 attgccggtg ggaagatttt ggctgtagaa gaacagctag aaggcggcta cgaaggagtg
     2101 gaagtagaag aactttcagg tgagggaaaa gtactaaccc cgggtttcat tgactgtcat
     2161 ttccatattt taggtggagg aggagaaggc ggttatcaaa atcgaacgcc agaagtcaca
     2221 ttgagccaat tgactacagc tggtgtaacg acagttgtcg gttgtttggg aacagacgga
     2281 gaaggtagag atatgactgc gttgatcagc aaagcgaaag gtctggaagc agaaggaatc
     2341 tccacttatg tatacgaagg ttcgtaccga ttgccggtaa aaacagtaac agattctatc
     2401 atcaaagact ttttgacaat tgataaaatt atcggaatcg gagaaatcgc agtatccgat
     2461 caccgttctt ctcagccaag ttttgaagag ttcgctcgtg cagtagcaga tgctcgtgta
     2521 ggaggcatgt tatccggaaa agctgggatc atcaatgtcc acttaggcgg aggaaaaaga
     2581 aagctagagc tattgacacg aatcgtagaa gaaacagaaa ttccgatcac acaatttttg
     2641 cctactcatg caaatcgcac accagaatta tttgaagctt gcgtggcatt tgccaaacgt
     2701 ggtggcacga ttgacttcac tgctagcgaa gatcctgatt tttgggagaa gacagatggt
     2761 gaagttcgct tcagcaaagc actgaaacga ttgatcgagg aagatgtttc tttagataat
     2821 ttcacgatga cctcagatgg acaaggaagc ttgccttatt ttgatgaaaa caatcatttc
     2881 ctagggttag gagtaggaag tgccaaggca ttgctagtag ggatcaaaga agctgtgcaa
     2941 aaagaaagta tccctttgga aatcgcctta cgagctataa ccagtaatcc agcacgtata
     3001 ttgaaattgg ataaaaaagg aaaaatcgaa attggtgctg atgcagatct gtgtatcttg
     3061 gataaagaaa cgcttgatat tgatacagtt atcgctaaag gagaaataat ggtccaagaa
     3121 aaagaagtca aagtatgggg gactttcgaa aaaagttttt aatcaacagc tctaactatc
     3181 ttttttagaa aacgagcgtt ctgtttgtct gtatctgttg ttgatgttac cctgaaggta
     3241 gatcaataaa caaactaaga ggaggaagca caatggcaga tttaaaagga cgttctaaag
     3301 atatgaaaga taaagtagtc ggtgaaggaa aagaagcata cggcaaagca acagacgaca
     3361 agagcaaaga aacagaaggg aaagcacaat cactaggtgc tgacatcaaa gaaaaagctc
     3421 g
//
LOCUS       DAXLPI010000174         3399 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXLPI010000174 DAXLPI010000000
VERSION     DAXLPI010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.180"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..277
                     /locus_tag="ACF3T5_002598"
     CDS             <1..277
                     /locus_tag="ACF3T5_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFD3633112.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="ACF3T5_002599"
     CDS             420..641
                     /locus_tag="ACF3T5_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633113.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="ACF3T5_002600"
     CDS             657..1499
                     /locus_tag="ACF3T5_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633114.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="ACF3T5_002601"
     CDS             1562..3364
                     /locus_tag="ACF3T5_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFD3633115.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1230 a          469 c          702 g          998 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DAXLPI010000175         3381 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXLPI010000175 DAXLPI010000000
VERSION     DAXLPI010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.181"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(167..397)
                     /locus_tag="ACF3T5_002602"
     CDS             complement(167..397)
                     /locus_tag="ACF3T5_002602"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFD3633116.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(491..829)
                     /locus_tag="ACF3T5_002603"
     CDS             complement(491..829)
                     /locus_tag="ACF3T5_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633117.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(1757..1873)
                     /locus_tag="ACF3T5_002604"
     CDS             complement(1757..1873)
                     /locus_tag="ACF3T5_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFD3633118.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            complement(2349..3227)
                     /locus_tag="ACF3T5_002605"
     CDS             complement(2349..3227)
                     /locus_tag="ACF3T5_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HFD3633119.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         1082 a          615 c          492 g         1192 t
ORIGIN      
        1 tctgaattta tatagaagtg tgtagctact tactaaatat acaaaagtta aaataaaata
       61 ctctggatac tctttatact gcattaaagt tagtaatatt aacagcaaag atatcgaaaa
      121 aacaataaca taatatgtgt caaaattttt cataataaga actcccttac tgttgcatac
      181 taacataatc caaaaactct tctacttcag ctaaagacat tccactttct ttagtccaaa
      241 aaacagtttt ttctatcgat ccttctctgt aaactgtaaa tgtcggagtc gagttaattg
      301 agtagcgact cttgaattct ttccactcat tttcatcttc tctaactttt gctacattca
      361 cgtaataaat attttctttt cccttttcta ccagcatatc gaatagcttc ctgcttttga
      421 tgatcgattt cttgtgctac atctagtaaa ttttcatcaa tttctatatc ataaagtatt
      481 tcactagtgt ctacatccac aatatatact ttttatcagt agcaattttt gagtaatccg
      541 ttaaaatgat ctcagtctcg tcactttcat cagtataaat atacgttaac aatgaactca
      601 ccttttcaat cggtgttagc tctaagccta ctgtattttt tactattgag tcattattta
      661 ccaccataac ttcgtactgt atataccatc ctatttggtt tccatcaata tcagtaattg
      721 gctcactagc taatggagtc tccggaacaa tattttcctg atatttctta aactcttctg
      781 tagcacgaaa aaggttatca tgttcctcac ttgaaactgg ttgagtcata gaaaatgtta
      841 cttggctaaa cagagggaaa ataaacacta caatcaacaa agtataaaaa aaagcccttc
      901 tttttcattt tttatcttcc aatcacaaat tgaaaaactt attcctgcaa ctttgagtct
      961 agtatcacaa tcgactgact acaactattc atttacattc tactttaagt tcctccttaa
     1021 ccattaattt tattaaagat acttgtaata gtgattctca caaaatttca tgaaaaatac
     1081 tttattactg tatctaaaat tccaatacac aacttatcga ctattatctg aaaaccaaaa
     1141 cttattattc cttgatgtta ttctagcaat agtaactgct tttttatttt tttgcatatt
     1201 ttttcggcgt tactttttaa agttaacttt ttcgacaaat agaaagagat acgaatcact
     1261 gatttttttg aagaaaagaa atggagtgag ctgaacgtta gagatattgg gagctttcgt
     1321 aactgcttat taaacgataa tggttatttg caaccgtatt gctcgcttct acatgaaata
     1381 tttcatgaaa tatttcgcaa aacctttgat tatgtagtta cgtttccatt cttgactaaa
     1441 aatgtttcaa aaacgaacaa aggtgttaaa aggtaaagtg atcaatacta ctggtcaaaa
     1501 taagaggttg aaagggaatt gaaaaataac tacagataat acttttataa acgtacgtgc
     1561 tttgtggttc tgtagttaac tatataactg gaattaggga atggtatcaa tttgaaaaga
     1621 aagattctac caatttatca tatgttaaaa atggaaaatc aacagcagtt ctttcggaaa
     1681 ccttacacaa aaaccgatta ttgaaaatag ccgaaaaacg tttttcggct atttttttaa
     1741 ttaagtttcc ttcttcttac cttttcccac tattccgttt cacatctttg atgatcttca
     1801 gcgcacgttt tcgtgttttg atatttgggc ttttcaagcg gcggtatgca cttgagatat
     1861 ctgttttttc catatggacg acccactttc ttctttgatt gtagtcatgc taaaaaacac
     1921 aaatcgaaac aattacgatt tgtgttttaa atggtatcaa aagttaaaat gaaagtcaac
     1981 ttgttaaaac agtcgatcag actagctgat tgactcctat ctctggaaat aaattttcat
     2041 tattacgttt tagctaccta aaaaaggccc tcttgtccgt taactttaat atctattgtt
     2101 atagctagtt tacctattga acgtactgtg tgttcgatag aacactaaaa aagaggctgg
     2161 gacagaagcg tttaactcca agaaacaaga agaaattcac gaaaattgct tttcaaattt
     2221 ttgtgaattt cagcttattt ccgaaggagt tgcttctgct tccgccgttt atacgtgttt
     2281 agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tcttttaaac tatttcgaag
     2341 tactaaactt atgaagtaag aatacctaag ccagagccaa tgtgctcgcc tgcaagcaat
     2401 tgctcttttg cttgtcgctg taaactcgta ctttctggat aatcatgtat ttgggcatct
     2461 actcgtccaa agatccaagg gactccacta tcgtcctgtt catcaactga taataatcga
     2521 taatcggctg caaaaagaca gatttttgac tcttttttca accatttagt aagattgggt
     2581 gatagtaacc aagtatctgt gtacatcact tggtaatggt aatcaggaaa ataaagttca
     2641 aaaaaacgaa tggcactttc gtaactatct cttatcttat tctgagataa atctgcatcg
     2701 gaaggaatat gaagagaaag gcactgcttt ccttctaatc ttagatccgt gtaccctttc
     2761 ggaaaatatg tcatttcgta ctccaattca ccaatccgaa agatcaagcc actagtgtaa
     2821 tgccaaatcc aaaaaccacg gtcaaagcga tagcttcctg ttacttggaa atgttcatga
     2881 ataaatctag taaaagcatt cattgttgcc aaatagatag ttcttggaat cttcaatggt
     2941 tgataaattt ttttccaact ttgagaagct gcatatagat aaatagctaa tgctgcaaaa
     3001 ggttcctctt catagacttt gtctattttc tttgcagtcg cctttgcttt attaggatcc
     3061 attagttcat gactatattg ctcaaacacc tcatcaagga tgttttttgc ttgtacaatc
     3121 atttgctcta taacgacttt gggaagagtt aaagtttctg ccagaatttg gatttcattg
     3181 cttattttct tcatcaaatt tccccttttc atatctttga acctcattgt ttaaatgact
     3241 gattatccgg acaccatatg gtataaacat acttttattg tacactaact ataaaactta
     3301 aactttctat gaagaaagaa tatctgtaag actttcaagg aaagcattga ccatctcagg
     3361 aaagtttatt cagttaccat c
//
LOCUS       DAXLPI010000176         3355 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXLPI010000176 DAXLPI010000000
VERSION     DAXLPI010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.182"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            104..1144
                     /locus_tag="ACF3T5_002606"
     CDS             104..1144
                     /locus_tag="ACF3T5_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFD3633120.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1968..2300
                     /gene="traE"
                     /locus_tag="ACF3T5_002607"
     CDS             1968..2300
                     /gene="traE"
                     /locus_tag="ACF3T5_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFD3633121.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2919..3260
                     /locus_tag="ACF3T5_002608"
     CDS             2919..3260
                     /locus_tag="ACF3T5_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFD3633122.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1249 a          457 c          639 g         1010 t
ORIGIN      
        1 aaaacagttt taatgcgttt gcatttttta tttatctttg ctattttcga aaagagtagg
       61 taattttaca taaataaaac aacgtgaaag tagaggatct acaatgactg attttaaatt
      121 ttttaaggcg gatagagttt acaacgaatt attttatcaa tttccaaaag tctttattgt
      181 ttctgacgaa tacaaaaaaa tgaaagattc aactaagatt gcctatatgc ttttaaaagc
      241 aagattagag atcgcaatca gcaaacggca aatcgatgaa gaaggtaatg tttattttac
      301 ttatacgaca aatgaactat gtagagtatt aaactgccaa aaacaaaaag cgatagcaat
      361 caaaaaagag ttggaatcct ttggtttatt attacaaaag cagatgggat ttaacaaaca
      421 gttagggaaa aataatccta atagactata tctagcagaa ttaaaagtct cagaaaatga
      481 tatctactta ctcgaaaaat ttgatagaga gaatagggaa aacgttgata aatcagaggg
      541 tatgaaaatc atacccaccc tcgacgaaaa atcagacgct gaatcccttg gggctcaaga
      601 gggtatgaaa atcataccgt gccaaaacgt tgataaatca gagggtatga aaatcatacc
      661 agaacttaat aataatatat tagacactaa tagacacaat atagacactg aaaaagaccg
      721 cctacaagat caattgttgt tagacaattt tgagacaatt atgacaaacg acagcattgc
      781 tacgtttgtc cctgaacgag tattaaattt gataaaaaca ttttcttcaa gttacagtga
      841 agctcaaaaa accgtccaga ctattcataa tgcaaagaaa aaagctgaaa tagaaagtga
      901 tatttcgata gtttttgaag aactcgatag ttattatgtc aatgcagaac aagaattata
      961 cacgacactg ttaaaagcct atcaaaaatt aaaaaccgaa aaagtcgaaa atatccagaa
     1021 cctgattttt gtctatgtaa aaaattggtt tatcgaaaaa ccaatagctg ctaaagtatc
     1081 aagtgaaaaa cgtttgaatt atgcaagctc cccaagcact attacgaaag actggttaga
     1141 gtgaaacttt actgacaaag tgattgttgc ttcatggagt aacattaaaa ttaaaaattt
     1201 tttattattc taattatatt acgaatatta caaaataata gctattttaa tagaatttat
     1261 aagttaaaat attatgataa aaatcacaaa cgtagccttt tacgtcataa gtgttaaaag
     1321 aaataattaa tttcaaatat agaaaaggga acgtatgttt gcatactcct tttttttgag
     1381 ttataataat acgcgaagag agataatttc attatttaaa caagctaaaa atcttgatat
     1441 tacttctttt caggcaatac gaatattatt cgcttacatt ttttgtataa atttaattgt
     1501 ctgctatgct tgtgatgaat tgattttcgt tttgcacttt tggatgataa atttggacaa
     1561 ttgaaaatac aaaaaaaaga aaggcggtat gaactcggaa ggctggtaac cttcgtcagc
     1621 gttcatacac ggcattaaga tttggccctt aatgcgttaa cctttcagtg ttacggtatt
     1681 acccgcaatg gatttagtat aacagatatt caaaagtgat accacttttt ttggctgtgt
     1741 taaggttaaa aatacgtgat attacgatga atctatgcct aagcataatg ttataaaaat
     1801 gtccgtgtgg ctctttgccg tatgacgaac gctgaaatta gagggaacgc atgtttggat
     1861 gcgttccctc ttttttgttt gtcatgaacg aaaatcaatt ctctattttt gaaaaataac
     1921 aagaaaaact tgttaaaaat atttaaagaa attgggtggt ttattttatg tcatatacag
     1981 gaattttgag tctcaaagat atttgtcact acggtaaacg gtgtaccgct acagagaaaa
     2041 tcacgaagaa actatcaact ggccaaagta aaaccgttgt gcagtgtaag aaatatataa
     2101 tccagaaaga caaagtctca gaggaaatga tttattacat tgggaaacaa aagcaaataa
     2161 ttttaaaaga tcctatcccc ttaaaagagc tatatccaac aatcaaacat gtttatgacc
     2221 aaaacggtgt actgattggt cgaagaaaaa atggagtctt gcgttgtacc gcaaaaggga
     2281 tggggcgttt gatcggctga attgatcatt ctcataactt gtattttatc gtttttcgct
     2341 tgcttgaaaa tattgtcgag acttctaaag gaacttagag tcactagaaa aataaggagt
     2401 agacttgatc tatcaacaat tcccttcggg gagtgacggg gtaggtttta acgattggta
     2461 acgtaaaaaa ttggtcaatc gagtaaatgc gcttgtttgg atagtggtga aatgaatttg
     2521 tgtaaataat aagcgcagag taaacaagag ggaatttaga agttagacaa gttagaatag
     2581 cgtctagatt aatcatgagc agctactaag ggatctagac tttgacgcaa actcatggaa
     2641 taaggagtag gtcttctcgt aatggggagg acgcacaaga aattgtgtcg tgtgctgcag
     2701 tacatgtaga tacttgcgag gacgttgatt attggatcaa tcgagcattt accgctgtct
     2761 actagggttt ctcgtttcgg acgccataaa cgagtcgaaa ttttttatgt atttttaagt
     2821 aaacgaaaaa cgataaaata tgaacaagtc ctgaataaag tgaaaaaatt aatataaagc
     2881 atagagaaaa tgaaatttac gtaatggagg aatgacaaat gaaaaattat gatccgaata
     2941 tacgattggg cacacatact attaaggttt ctttccaacg ttgggattac aaaggatttg
     3001 tgacgtttag aaggggcgga aattgtaaag gattagatgt tttagcatta gacgaggatg
     3061 atttatacga tcaaacattg actgataatc caattggatt cggcttgcta cctgaagatg
     3121 atgaaggtaa tgaatggttt aaaatgacgc ttatgaatga taatggagac gaattatctg
     3181 ttgaagatac ttggtcatat ttgagtgatt atattgtcag ctttgaaatc attgaatttg
     3241 tagcggataa agaagaataa aaactggagg aataaatttt acagataaag gcttagaagg
     3301 agtcacgacg attaataaaa aggtagtatt accttaggca ttgagtaagg aattt
//
LOCUS       DAXLPI010000177         3328 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXLPI010000177 DAXLPI010000000
VERSION     DAXLPI010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.183"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..354
                     /locus_tag="ACF3T5_002609"
     CDS             <1..354
                     /locus_tag="ACF3T5_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HFD3633123.1"
                     /translation="RATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSS
                     LETPSISAKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKLESLQ
                     PPIPPIPKTPKLPGL"
     gene            791..1108
                     /locus_tag="ACF3T5_002610"
     CDS             791..1108
                     /locus_tag="ACF3T5_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633124.1"
                     /translation="MEKTKKLQLEDFTENEFFGTQEQQYLKAQVREELKEQGFIIDSS
                     FEGDFKTWIGVYARPKDKPTYLDPQNDKEAEEQEQYSINGFKQDFSEWFEWEIKNLKI
                     KEM"
     gene            1147..1644
                     /locus_tag="ACF3T5_002611"
     CDS             1147..1644
                     /locus_tag="ACF3T5_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFD3633125.1"
                     /translation="MKYIKNVETLEELKKAYKKLALKLHPDCGGSNEEMAQLNNEYDE
                     LFVKLKNTHKNKDGETYTKETTETPEQFKDIINQLFNLKMDGVSIEIVGTFIWLTGNT
                     KPYKDDIKALEFRYSPKKYAWYKAPSDYKKRSRKNYDMDTIRGMYGSQKVKEDKEEKK
                     YLQAN"
     gene            complement(1734..1925)
                     /locus_tag="ACF3T5_002612"
                     /pseudo
     CDS             complement(1734..1925)
                     /locus_tag="ACF3T5_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281642.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1849..2121
                     /locus_tag="ACF3T5_002613"
     CDS             1849..2121
                     /locus_tag="ACF3T5_002613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633126.1"
                     /translation="MLNALELNRGFLFLWSFSLKIILARKNGTLLEALRAELPAFEQT
                     IVPEFKGAVRAVLKKIHRPSIGVKMGEIANYSSTSSPMGQLKKMFG"
     gene            2292..2870
                     /locus_tag="ACF3T5_002614"
     CDS             2292..2870
                     /locus_tag="ACF3T5_002614"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3633127.1"
                     /translation="MKTKDLIIEEFIKSFGENGFEGTSVDYLAKQVGIKKGSIYTHYS
                     GKNEIFTIACEKILDKCIVAIKQLLEVERDKPLDEQLFILLKTVCLESDFLTADEIAF
                     SKRVAILSSSELLSLVDKLLAKYDNFIDDTVSRIFIDCAKQKMLAEEEITSAISVYRM
                     LLENLYIDSLANQNCNEKLNHLWRYFWKGVCS"
     gene            2902..>3328
                     /locus_tag="ACF3T5_002615"
     CDS             2902..>3328
                     /locus_tag="ACF3T5_002615"
                     /inference="COORDINATES: protein motif:HMM:NF018410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HFD3633128.1"
                     /translation="MEIIKYIGAAFSTWFIGFFPYFEIYIAIPAGFAAGLNWFDAFLW
                     ASLGNWMVIPFVDICYEWLMKFNFMKKIMEKSLNGKWENRIEKHGAWVILLLTPLAGV
                     WTIGVIAKALKFNRAKLWIYSATSVGITGLIITILIINGI"
BASE COUNT         1290 a          477 c          613 g          948 t
ORIGIN      
        1 agggcaacac caaaacaagc gcatgatatt gttgtcaaaa acttaccgac caatttagaa
       61 acccttcata aaacgggctt atttagcgat ataaggcttt acaatagaga aggagtgaaa
      121 ctctattcaa gcttagaaac gccttccatt agtgcgaaag agaccttaga aagagaatta
      181 aatcgtaaag tatcagggaa agaaattcaa ccgactttgg agagaataga gcaaaaaatg
      241 gttcaaaatc aacaccaaga aacccctgaa tttaaagcaa ttcaacaaaa attggaaagc
      301 ttgcagccac ctataccacc aatacccaaa acacctaaac ttccaggact ttaaacctta
      361 aaaaaggaaa agagtagtta ccaaaaaacg gtaactactc tttttttata aaaacctttc
      421 ctcatgtttg aaaagctgat cgagaaataa agcacacaac ggtattcttt cttttttcag
      481 ttggtacatt ttgcagtctg taacgtgtca tttactgttt gttaccttaa catatccagt
      541 cgaaaacaca agggtatata aacgagcaag ctcgcccttg tgttttcttt gtgtctatgt
      601 tgcgttctcg tcacagcaaa cgacacgaac acactggcga aaatgaacca actgaaaaaa
      661 gaaaggagca tacactgtaa tttatttcta gcttttaaaa catgaggaaa acagcttcac
      721 ttcctcaaac gcctaacaaa aatggttgtc aaaaaactaa aaatcaaaaa aaagaaaaga
      781 ggaaaagaaa atggaaaaaa caaaaaaatt acaattagaa gattttacag aaaatgagtt
      841 ttttggaaca caagaacagc aatatctaaa agctcaagta agagaagagc taaaagagca
      901 aggctttatc atagatagca gttttgaggg cgattttaaa acatggatag gtgtatacgc
      961 aagaccaaaa gataaaccga cctacttaga ccctcaaaac gataaagaag cagaagaaca
     1021 agagcaatat tctattaacg gctttaaaca agattttagc gaatggtttg agtgggaaat
     1081 aaaaaatttg aaaattaaag aaatgtaaaa aaaagccaaa aatcaaaaag aaaaggaatg
     1141 atgaaaatga aatacattaa aaacgttgaa acattagaag aattgaaaaa agcctacaaa
     1201 aagttagctt tgaaattgca ccccgattgt ggtggtagca atgaagaaat ggcgcaacta
     1261 aacaatgaat atgatgaatt gtttgtcaag ctaaaaaaca ctcataagaa caaagacggc
     1321 gaaacctata caaaagaaac gactgaaacg cccgaacaat ttaaggacat tataaatcaa
     1381 cttttcaatc tgaaaatgga cggtgtatca attgaaatcg tgggaacttt tatatggtta
     1441 acaggcaaca caaaacctta taaggacgac ataaaagccc tagaatttcg ttattcacca
     1501 aaaaaatacg catggtataa agcaccaagc gattataaaa aacgtagtcg aaaaaattat
     1561 gatatggaca caataagggg aatgtatgga agtcaaaagg tgaaggaaga taaagaagaa
     1621 aaaaaatact tacaagccaa ttaaggcttg taagtttgtc agaaaggaag ttttaaagtg
     1681 gatattaaaa taaaaaaaat aaagggtatt cggattttat ggtggaaaag ggcctataag
     1741 ttactctttt ccaatttctt aataagtgtg gaaaaatcta caccatagtt gcctgttttg
     1801 tctcggtctg ttatttgata cgaattgaaa ctatcgtata acatagcttt gctgaatgca
     1861 cttgaattaa atcgtggctt cctcttttta tggagctttt cattaaaaat tattttagct
     1921 cgtaaaaacg gaactctttt agaagctttg agagcggaac tacctgcatt tgaacaaacc
     1981 attgtccctg aatttaaagg ggctgttaga gctgttttga aaaagattca tcgtccttct
     2041 ataggggtta aaatgggtga aatagctaat tatagttcca catcaagtcc aatgggacaa
     2101 ctgaagaaaa tgtttgggta acgaaacaca cagaatgagc aattttaata ttaaaattga
     2161 agagttacca tctctagaga aaagtttccg ccaaagtatt gacacataca aatcaccact
     2221 attacttgac aaatggaatc acctagtata aaatactaac taccggtagt tagtattaag
     2281 gatggtgaat tatgaaaaca aaagatttaa ttattgaaga atttataaaa tcctttggtg
     2341 aaaatggatt tgaaggaact tctgttgact atctagcaaa gcaagttggc ataaagaaag
     2401 gttctattta tacccactat agtggaaaaa atgaaatatt tactattgcc tgtgaaaaaa
     2461 tactagataa atgtatcgtt gctataaaac aattgcttga agtagagagg gataaaccat
     2521 tagatgaaca actttttatt ttgcttaaaa ctgtctgtct agaaagtgat tttttaaccg
     2581 ctgatgagat agctttcagc aagcgcgttg caattttatc tagcagtgaa ttgttgtcgc
     2641 ttgtagataa attattagca aaatatgata attttattga cgacacagta tcacgaattt
     2701 ttattgattg tgctaaacaa aaaatgttgg cagaagaaga gataacgtct gccataagtg
     2761 tatatcgtat gttgttggaa aacttatata ttgattcatt agcaaatcaa aattgtaatg
     2821 aaaaattgaa tcatttatgg agatattttt ggaaaggtgt ttgcagttaa aaattaaata
     2881 aacttgattg gaggtttaat tttggaaatt attaagtata ttggagcagc attttcaact
     2941 tggttcatag gtttttttcc ttattttgaa atttatattg caatacctgc aggttttgca
     3001 gccgggctaa attggtttga cgctttttta tgggcttcac taggaaattg gatggttatt
     3061 ccgtttgtgg atatatgcta tgaatggtta atgaaattta atttcatgaa aaaaatcatg
     3121 gaaaaaagtc taaatggaaa atgggagaat cgcatagaaa agcatggtgc atgggttata
     3181 cttttgttaa ctcctttagc aggtgtttgg acaataggtg ttattgctaa agcactaaag
     3241 tttaatcgag caaaactttg gatatatagt gcaactagtg ttgggattac tggattaatt
     3301 attacgatat taattataaa tggaatta
//
LOCUS       DAXLPI010000178         3265 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXLPI010000178 DAXLPI010000000
VERSION     DAXLPI010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.184"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            179..775
                     /locus_tag="ACF3T5_002616"
     CDS             179..775
                     /locus_tag="ACF3T5_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFD3633129.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="ACF3T5_002617"
     CDS             899..2698
                     /locus_tag="ACF3T5_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFD3633130.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="ACF3T5_002618"
     CDS             complement(2976..3188)
                     /locus_tag="ACF3T5_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633131.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
BASE COUNT          921 a          578 c          594 g         1172 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagtg tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacg
//
LOCUS       DAXLPI010000179         3128 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXLPI010000179 DAXLPI010000000
VERSION     DAXLPI010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.185"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            189..452
                     /locus_tag="ACF3T5_002619"
     CDS             189..452
                     /locus_tag="ACF3T5_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFD3633132.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            452..724
                     /locus_tag="ACF3T5_002620"
     CDS             452..724
                     /locus_tag="ACF3T5_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFD3633133.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            801..1130
                     /locus_tag="ACF3T5_002621"
     CDS             801..1130
                     /locus_tag="ACF3T5_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFD3633134.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            1120..1407
                     /locus_tag="ACF3T5_002622"
     CDS             1120..1407
                     /locus_tag="ACF3T5_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFD3633135.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKIIPVLLCGTHEKFYEQLKKLI"
     gene            1692..2549
                     /locus_tag="ACF3T5_002623"
     CDS             1692..2549
                     /locus_tag="ACF3T5_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFD3633136.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            2550..3098
                     /locus_tag="ACF3T5_002624"
     CDS             2550..3098
                     /locus_tag="ACF3T5_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633137.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
BASE COUNT         1178 a          417 c          623 g          910 t
ORIGIN      
        1 tttttgttga tttaccaaca tgtttagaca ttttcctcta ggaaaaaatt aactagcacc
       61 acgggtataa aggaaacgga taacacttta caatgctgtt ttggtactcc aatttaaaca
      121 aaacaatcga atttacgtac ttaattaagt acgttagaat ggagatataa attagagggg
      181 gagtagatat ggcaaaaaca gtcttaaatc ctagctcagc acggaaaaat ttttataagt
      241 tgttgaaaga tgtaaatgag aatcatacgg aaattgaaat cattagtgag cgcagtgaaa
      301 ataatgcggt gcttattggt ttggatgatt ggaaagcaat caaagaaacg ctattacttg
      361 agcaaatagg cactttgggt gttgttcgta agcgagaaaa agatgatagt ggatttacta
      421 atgttgatga aattgattgg gataacttat aatgaataac tattctgtga tgattaaaaa
      481 ctcggctaaa gctgatttaa agaaaatcaa acagtctaat ttgaaacctc aatttgagaa
      541 ggttattcag actttaaagg aagatcccta tctccctacc caatcatttg agaagttaaa
      601 acctacacat gaaggtaggt attctagaag attgaataga caacaccggg ttgtttataa
      661 ggtagatgaa gatgaacacg tggttgaaat ttattcagca aggacgcatt acgagtcaca
      721 gtaattactt gtaaatacat tatctcgtgg tataatgtat ttactaagta attacatact
      781 gttagaaatg agggatttaa atgattacaa aaacaagaaa acaaggcaat tcaatcatgt
      841 tgacagtacc taaagatttt aatgttccta atggagtcga agttgaagca aagttggtag
      901 agaatggtat tctttatgaa tttgttgaac cacaaaaaga attttatgat tttagcgaag
      961 atattttgtc tgatataatt gcagaaggct acgataaaga tgagattctt gtagaattta
     1021 agaatagaaa aaacaaaatg cattcttcct ttagagatat tgctgaagat acacttacta
     1081 attctaaagt aatgactaaa gaggagttgg caacagaaat tggcttatga aatcgttcct
     1141 tcaaaacatg tcattaagta tttaaaaaag ctaaaagaga agccattgaa agaaaaattt
     1201 ttgaatttaa tttatgatga aattgcgatt gatccatata agggtgaaca aaaaactggg
     1261 gatttatcag gtatttggtc tagtgggttt aaatatgcgg gcacaacata tagagtagct
     1321 tacgagatta tagaaaacaa gattattcca gtattgttat gtggaacgca tgaaaaattt
     1381 tatgaacagt tgaaaaaatt aatctagtga tttgttgtcg ttcttaagaa ggacggcttc
     1441 ttttttatcg aaaatctgat tcgtctacat gactaaagtc gcgagtattc tctgcttctc
     1501 gataaattaa ataacgtcat tactttgagg aaagctataa atcaatatat ttcagctttt
     1561 ccttatttat gtactacctt ttccttatta attataaggt agtacatata tatctttttt
     1621 tggtagtaac taaaaataaa tatgttataa tagtatcaaa tgaatattgc taaaagaaaa
     1681 gaggtaggaa cgtggtagcg aagaaatatg taatcggtaa ttttaaaggt ggcgtaggta
     1741 aaagtacgtg cgcgcaaatg ttagggtttg aatcagcagt taatgaagga aaaagaacct
     1801 tgataattga cttggatatg caaggaaata cttctgatgt gatgaactta acgcatatga
     1861 acttcactaa cgaagaagga ggcggagaag gtgaaccgat tgtttacgaa aatactgtga
     1921 ctgacgtgtt gataaacaat cttccaccta aagaggctgt ctataaagta atcaataacc
     1981 tctatatact tccagcagac atgtcctttg aaatgtatga tgattggatc aaagaccaat
     2041 ataatgattc attatcccaa ttcaaatata tgaaagaaaa gctagaacca ttatttgatg
     2101 agtttgacgt catctattta gatgtcccac cttctatttc tgtatatagt aaatccgcga
     2161 tgtacattgc ggactgggca atcgtcgtct tacagaccca agtaaaatcg atgagaaatg
     2221 cgatgcaata tcttgagtat atggactttt ttgtgaaaga atttgataca aatttatatg
     2281 ttgctggtgt tattcctttc atgttggaaa gcggtgacgc agtagatcag gaaatgtata
     2341 aacaagccca agagatttat ggggaacatt tgattaaaaa tgttgtactc aaaaatgcgc
     2401 gcttaaaacg atacgacgga tcaggtatta cgatggagaa gacaaagaaa ggtctcttaa
     2461 aacaatggga tcgcaaaagc cacgaattat ttatcagtat tttgaaagaa ttggaagaac
     2521 acgagaattg gtatgcggag gtggagtaat tggctgacat caaaggactt gatatttttg
     2581 ggaacaataa aggactcgcc aaaataaaaa aagaagcaga agcaaagaaa aagcagcaag
     2641 caattacacc atctgaaaat aagcaattag aagtgcacca agttcaagaa ataaaaactt
     2701 cctccaatgt tgaaagacag agtagaggaa gaacaaaaaa agttggcgca ccaatcagaa
     2761 agtttgataa ggtttattcc accaagcatc ctttaaaact gtcgactttg ttaaatgcga
     2821 cttccagagt tttagtagaa aagtatgaga ctaatatgac aagagatgaa ttgcttagaa
     2881 aagctttaga tgagtatatt aaacagaatc ttacgcaaga agataagcaa gatttgttta
     2941 atgacgtggt gaaagaacta gacttgttca gagaaaaata tccaattgtt ccagaattag
     3001 atgatcgtgg agaaataata agaactacca gtcagataaa acaagatacc gaagactcac
     3061 tgcgcaaagg gtggggaatg aataataaag ttagataata atcaaagaag aactaagtaa
     3121 ctaccttt
//
LOCUS       DAXLPI010000180         3023 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXLPI010000180 DAXLPI010000000
VERSION     DAXLPI010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.186"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(287..649)
                     /locus_tag="ACF3T5_002625"
     CDS             complement(287..649)
                     /locus_tag="ACF3T5_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633138.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACF3T5_002626"
     CDS             complement(723..1103)
                     /locus_tag="ACF3T5_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFD3633139.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACF3T5_002627"
     CDS             1185..2360
                     /locus_tag="ACF3T5_002627"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFD3633140.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACF3T5_002628"
     CDS             complement(2437..3021)
                     /locus_tag="ACF3T5_002628"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFD3633141.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          578 c          498 g         1005 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tat
//
LOCUS       DAXLPI010000181         2946 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXLPI010000181 DAXLPI010000000
VERSION     DAXLPI010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.187"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..278
                     /locus_tag="ACF3T5_002629"
     CDS             <1..278
                     /locus_tag="ACF3T5_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFD3633142.1"
                     /translation="LLLNIWIFDRSRNSSIGFRTKRSLSSKKNWVYSQTIFYGGIVLI
                     SLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(369..734)
                     /locus_tag="ACF3T5_002630"
     CDS             complement(369..734)
                     /locus_tag="ACF3T5_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633143.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            942..1424
                     /locus_tag="ACF3T5_002631"
     CDS             942..1424
                     /locus_tag="ACF3T5_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633144.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1621..2511
                     /locus_tag="ACF3T5_002632"
     CDS             1621..2511
                     /locus_tag="ACF3T5_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFD3633145.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(2798..>2946)
                     /locus_tag="ACF3T5_002633"
     CDS             complement(2798..>2946)
                     /locus_tag="ACF3T5_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3633146.1"
                     /translation="AFKVSHSNGVTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGL
                     IFEN"
BASE COUNT          997 a          475 c          576 g          898 t
ORIGIN      
        1 tattacttct gaatatatgg atttttgata ggagtagaaa ttcaagtata ggttttagaa
       61 ctaaacgaag tttgtcttct aaaaaaaatt gggtatactc acaaactatt ttttatggag
      121 gaattgtttt aattagttta ctttcttcaa cactttattc tttgaatatt atagatgttt
      181 ccacctcaaa ttcaatatct attattggaa tcattattgc tgcaattatt acacaactgt
      241 ttttagtgtt tggagagaag aaaagaagta aaaaatagaa aaactagtga tagttttggt
      301 gatataagaa aaacatatat gtataaaaag aagaaagtag cttctctttg tacatatatg
      361 gtttgttttt atggatgccg agctttttta taagataagg ctaccgccca taacgaccca
      421 aggatttgtg gaactaagta aatacctgca agaagcaaga tcagctgggt cggttttaaa
      481 ggatgataga agtaacgcca ttcagtaaaa ttggcgcaaa ataaatagga agtgacagta
      541 gatataatat tggcaatgaa taaagatata atacgcctgt tcctttgaaa ataatatcca
      601 gcaaaaagag ataaaaatat caatgtgatg aacccgctta cggaatgata acgaaaatca
      661 agcagtaagg cgaaatgcac ataaggaaca aaagataaaa gcaacggaag gtttttccag
      721 caagttttta tcatctttca tcaccaccta acaagtgtta ttattttgtt agttttattg
      781 taaattgaat cctgcaataa tcaattactt tttcattatt tctagattaa atgaataaac
      841 tctcatgcca ttatctctaa aaaaaaagtt tttcccccta atttttttag atagaaaatt
      901 gtatagtatg atgagagaaa aagtagtttg ggggaacgtt aatgagaaga aaagcagtaa
      961 tcgatcggct ttatttattt ttttggtttt ttcctatcgt tttgctacct tttacatggg
     1021 taaatgctgt tgtacaaggt gcggagatat taggcctgat ccaaggaagc ggatttgtgg
     1081 tttctaggaa ttttccagct ttgattgttt tctatttagt gataagtgta ggtctcttat
     1141 taccaaaatc tctgatcaat cactacaatt ggcagctgct ttacttttct tgcttattag
     1201 tatttacatc tatgttttct ttgctgcctc gcttgattat cggccctaat ggtttgagcg
     1261 agagtggatt gaaggattta gcttattcct ccatgtattt cttgacgatc aaaccatggt
     1321 tctatttagt aattgcctca tttattcttg caacattttt ttacttctgg acagaaatca
     1381 ggataaaaag tgaacgatct gtatctataa aaaataacta ttgatggtgg atcatcagta
     1441 aaaaaacata tgagctgcgt aaaatgcatg tttctcatat gcttttttta ttattatgta
     1501 aagaatgcga aaaacgttta catgattttt acataactta actatatttt tacagcaacc
     1561 atacgaggga tttacagtcg actgataaac tttagttgta aataagaaag gagcaataaa
     1621 atgaaaaaag tattgtttac tctaggtatg atgggaattc ttttagccgg atgcggctca
     1681 ggatcaggaa atacagatgg ctctgccaca caaaataatt caagtgactc aaatgaacaa
     1741 gtaaaaatcg ttgcggtagg ttcgacagca ttacagccgt tagtcgatgc agcacaagaa
     1801 agctttgtac aagaaaatcc aaattatcaa atctctgtac aaggaggagg aagcggaact
     1861 ggtttgagcc aagtcgaagc tggagcagtt actatcggaa actctgatgt atttgcagaa
     1921 gaaagagacg gagttgatgc ttcaaaatta gtggatcata aagtagcagt ggtcgggatg
     1981 gcaccaatcg tcaataaaga tacagatgtc aaagatatca ccaaacagga attgattgat
     2041 atctttacag gaaaaatcac taactggaag gaagttggcg ggaaagatca aaaaatcaac
     2101 gtcgtgaacc gtgcaaacgg cagcggtacc cgcgcgacat tcgaaaaatg gggactggat
     2161 ggtgctaccc ctgtgcagtc ccaagaacaa gattcatcag gaacagttcg gcagctggta
     2221 agtcaaacac caggagcaat cagctattta gctttctcat atctcgatga ttccacacaa
     2281 gcactaagta tcgacggtgt agaaccaaaa gaagagaatg tagcagataa cagctgggga
     2341 atctggtcat atgaacatat gtacacgaac ggaaaacctt cccctgaagt ccaaaaattc
     2401 ttagactaca tgatgacaga agaaatccaa gaaggacctg tcaaagaatt gggctattta
     2461 ccaatcacaa tgatggaagt agaacgtgat cacgaaggaa atatcaaata actagcagat
     2521 aaacatgcca cggtagtcgt tactattaag gaaaaatcaa ctatgtttcc ccacatatac
     2581 atttttcctt taattgtgaa aaatagttcc tcatattttg ccgttttttg agctaatacc
     2641 gagggtcagc tgattgaaca ccgtttattc gaaaaaaaga gctacgacat agaactgtcg
     2701 taactttttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
     2761 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacctt
     2821 gttgtaaata gatccgtgcc cgaaagttat aaaagttgcg ataaccaaac gcaattcgtt
     2881 taatcagctt gatcttgtta ttcagcccct cagtgacgcc gtttgagtgg gacaccttaa
     2941 aggcat
//
LOCUS       DAXLPI010000182         2943 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXLPI010000182 DAXLPI010000000
VERSION     DAXLPI010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.188"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            73..768
                     /locus_tag="ACF3T5_002634"
     CDS             73..768
                     /locus_tag="ACF3T5_002634"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3633147.1"
                     /translation="MIKAVIFDLDDTLVSEYDYVKSGYKCVARRLAVQKIFPATFDEI
                     YRVLLDLFAEDSKNVFNRLYDYFEIPYSKKEIKELVNVYREHKPDINFLENADTLLTE
                     LRSRGYKLGIITDGYAITQRNKLDALKVDYLVDYICITDELGRELWKPHPISYLLLLE
                     KFNLKAQECIYIGDNELKDFITAKSIGMQTIKIERVNGIYRESSLKFKRFYQADKKIR
                     SLWEIKNFLGVIS"
     gene            765..1883
                     /locus_tag="ACF3T5_002635"
     CDS             765..1883
                     /locus_tag="ACF3T5_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HFD3633148.1"
                     /translation="MKSKRILLASPHMSEEGYEKEYVKEAFDTNWVAPLGANVNGFEK
                     ELAEYVGASHAAALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPILYQGAIPV
                     FVDSDYETWNMKPEHLRTALEKYPNTKAVLVVHLYGLSANLDEITKICKEYDVTLIED
                     AAESLGTTYKGKYTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSR
                     EKARHYQHNEIGYNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQF
                     MPENNWNEPNYWLSVITIKGINANHLIDELDKNNIEARPVWKPMHLQPVFKNYDYIGD
                     DVAETLFDLGVCLPSDTKMTDEDLKYVVSTIKRVINDA"
     gene            1873..2493
                     /locus_tag="ACF3T5_002636"
     CDS             1873..2493
                     /locus_tag="ACF3T5_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFD3633149.1"
                     /translation="MMRKKTFYQLFGKRILDILLSGIALIVLSPIILIVGFLVRIKLG
                     SPIIFKQERPGKSEKIFSMYKFRTMTDERDHNGEYLPDEIRLTKFGKMLRATSLDELP
                     ELWNILKGDMSIVGPRPLLVEYLPLYSEKQRKRHNVRPGLTGLAQVNGRNAISWEEKF
                     DLDVYYVDKISFFNDLIIIIQTCKKVIKKENINTINNNIPEKFKGS"
     gene            2689..>2943
                     /locus_tag="ACF3T5_002637"
     CDS             2689..>2943
                     /locus_tag="ACF3T5_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3633150.1"
                     /translation="MAKYYNKKILIMANNDDGLYRFRGMLIKNLVDQGNEVIVSLPKG
                     KNIPKLIELGCQIIETPIDRRGINPKTDFKLFFNYCNILKK"
BASE COUNT         1128 a          360 c          554 g          901 t
ORIGIN      
        1 attatttaga taaaatgata gcactgagat atgatcaagc gatttattta gatgaaaaag
       61 gaaatgtata taatgattaa agcggtgatc tttgatttag atgacactct tgtgtctgaa
      121 tatgattatg taaaaagtgg atacaaatgt gtagctagaa gattagcagt gcaaaaaatt
      181 ttcccggcta cttttgacga aatatataga gtccttttag atctttttgc tgaagatagt
      241 aaaaatgtat ttaatcgtct atatgactat tttgaaattc cttatagtaa aaaagaaatt
      301 aaagaactgg taaatgtata tcgagaacac aaaccagaca taaatttttt agaaaatgca
      361 gatactttat taacggaatt aagaagtaga ggatacaagc tggggattat tacagatggg
      421 tatgctataa cccaacgaaa caaattggat gctttgaaag tagactattt ggtagattat
      481 atttgtatta ctgacgaatt aggccgtgaa ttgtggaaac cacaccctat ctcatatctt
      541 ttattgttag aaaagtttaa tttaaaggca caagagtgta tatatattgg cgataatgag
      601 ttgaaagatt ttataactgc caaatcaata ggaatgcaaa caattaaaat agaaagagta
      661 aatggtattt acagagaaag cagtttgaag ttcaaaagat tttaccaagc tgataaaaaa
      721 atacgttcgt tatgggagat aaaaaatttt ttgggagtga tttcatgaaa agtaaaagaa
      781 ttctattagc ttctccacat atgagtgaag aaggatatga aaaagagtat gtaaaagaag
      841 catttgatac aaattgggtt gctcctttag gtgcgaatgt gaatggattt gagaaagaat
      901 tagcagaata cgtgggagct agtcatgctg ccgcattatc ttctggaaca gcagctatac
      961 atttagcttt gaaagcggct ggggtcggta aagatgacat agtgatttgt cagtccttga
     1021 cgttctcagc tactgctaat ccaattctct atcaaggagc tatacctgta tttgttgata
     1081 gtgattacga aacttggaat atgaaacctg aacatttgcg aacagcacta gaaaaatatc
     1141 ctaacacaaa ggctgtatta gtagtacatt tgtacggttt atcagctaat ttagacgaaa
     1201 ttactaaaat atgcaaagaa tacgatgtta ctctaattga agatgctgca gaatcattag
     1261 gaacaacata taaaggaaaa tatactggaa catttggcga ctatggaatt tattctttta
     1321 atggaaataa gataatcaca acttcaggtg gcggaatgct tgtatccaat gacaaagaaa
     1381 aggtggaaaa agtgagattt tgggctacgc aatctcgtga gaaggcacgc cattatcaac
     1441 acaatgaaat tggctataat tatcgaatga gcaatatcgt tgctggtatc ggtcgaggac
     1501 aattgaaagt attaaatgaa agaattgaga aaaaacgata tattcatagt ttttataaaa
     1561 aagaattgtc taaattgtca aatatacaat tcatgccaga aaataattgg aatgaaccga
     1621 attattggtt aagtgtcata acaataaagg gaataaatgc gaatcatctt attgatgagt
     1681 tagacaaaaa taatatagaa gcccgcccag tatggaaacc tatgcattta caacctgttt
     1741 ttaaaaatta tgattatatt ggcgatgatg tagcagaaac tttatttgac ttaggagtat
     1801 gtttaccatc agatacaaag atgacagatg aagatttaaa atatgtagta tctacgatta
     1861 aaagagtgat caatgatgcg taaaaaaact ttctatcagc tttttgggaa aagaatttta
     1921 gatatcctat tgtcaggaat tgctctcatc gttttatcgc cgataatctt aattgtaggt
     1981 tttttagtaa gaattaaact tggttctcca attatcttca aacaagaacg accaggaaaa
     2041 agtgaaaaaa ttttttccat gtataaattt cgtacaatga ctgacgaaag agaccacaat
     2101 ggagaatatt tacctgatga aattcgacta actaaatttg ggaaaatgct tagagcaaca
     2161 agtctggatg agctacccga actttggaat attttgaaag gtgatatgtc gatcgtagga
     2221 cctcgtccat tattagtgga gtatttgcca ctatatagtg agaagcaacg gaaaagacac
     2281 aacgtaagac caggattaac tggactggct caggtcaatg gaagaaatgc tattagttgg
     2341 gaagaaaagt ttgatttaga tgtttattat gttgataaaa ttagtttctt taatgatctt
     2401 attattataa ttcagacatg taaaaaagta atcaagaaag agaatattaa tacaatcaat
     2461 aacaatatcc cagaaaaatt taaagggagt taaatacagc agcttaatgc aagttttgtg
     2521 acttgcatta tatttaggta taaatcaaag cgagtatatt agaattatat aacatatgtt
     2581 aaaaaaacgt atagctaata aaagtatgat tttaaaaatt tgtgtagagt tttaaatata
     2641 ctgtttcaat acgagaaaat tataaaaaag tataaggaaa agagagttat ggcaaagtat
     2701 tataataaaa aaattcttat aatggcaaac aacgatgatg gtctttaccg ttttcgagga
     2761 atgctcataa aaaacttagt agatcaaggt aatgaagtta ttgtatctct tccaaagggc
     2821 aagaatattc ctaaattaat tgaacttgga tgtcaaatta ttgaaactcc aatagataga
     2881 agaggaatta atccaaaaac tgactttaaa ctatttttta actattgtaa tattttgaaa
     2941 aaa
//
LOCUS       DAXLPI010000183         2930 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXLPI010000183 DAXLPI010000000
VERSION     DAXLPI010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.189"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(61..1371)
                     /locus_tag="ACF3T5_002638"
     CDS             complement(61..1371)
                     /locus_tag="ACF3T5_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFD3633151.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="ACF3T5_002639"
     CDS             complement(1383..2801)
                     /locus_tag="ACF3T5_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3633152.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
BASE COUNT          924 a          607 c          453 g          946 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa
//
LOCUS       DAXLPI010000184         2759 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXLPI010000184 DAXLPI010000000
VERSION     DAXLPI010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.190"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(12..191)
                     /locus_tag="ACF3T5_002640"
     CDS             complement(12..191)
                     /locus_tag="ACF3T5_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633153.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(178..516)
                     /locus_tag="ACF3T5_002641"
     CDS             complement(178..516)
                     /locus_tag="ACF3T5_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFD3633154.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(532..735)
                     /locus_tag="ACF3T5_002642"
     CDS             complement(532..735)
                     /locus_tag="ACF3T5_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633155.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(750..1526)
                     /locus_tag="ACF3T5_002643"
     CDS             complement(750..1526)
                     /locus_tag="ACF3T5_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFD3633156.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1825..2145
                     /locus_tag="ACF3T5_002644"
     CDS             1825..2145
                     /locus_tag="ACF3T5_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD3633157.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            2163..2594
                     /locus_tag="ACF3T5_002645"
     CDS             2163..2594
                     /locus_tag="ACF3T5_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFD3633158.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDK
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT          847 a          486 c          430 g          996 t
ORIGIN      
        1 tctattcatt tttagtcagc cccctcggtt ggctttttcg ctctgtactc agcttcagct
       61 tcatcaagcc ccataaaaat ccaaaccatg taaacaatcg ttcctatcaa cgcttgcctg
      121 cttccccaaa gtcctaaagc gtagatgatt agcggtgcgc tgaacactag cgctctatta
      181 aattttccca tccgcttgcc tccttaagat ttccgaaaaa tttgtttcta aaaattcaag
      241 tgttttactt cttaaaaata gatatgtgtc tcttccttca actggataat agacaaatcc
      301 atttttgttt ttctcgatat cgataatatt tctatatctt gggttcttta aaactctaga
      361 agtaaaccaa tcatattttc tgttaatccg ttctagcact tctggcaacg tcatccatct
      421 accagtatca tcagcttttt ttaactcctc ataatccact tgggagataa ttacatagcc
      481 ttctggaatt gggatttttg cttctagata ttgcattagc tgttcctccc tttacaattc
      541 gtacatagtg ataatcgaat ctataattct gtttgcttct gcagaagtct ttttaccgtt
      601 taaaattaaa gataagtagc ttttgctaat tccaaatctt tcagcaagca tggtgtaagt
      661 taagaacttt gaactttcga catattcttt gattttttct ctatctcgtt gagtgatttc
      721 tgcaatgtca gtcatactaa aactcctttc taaccaattt cctctaaatc catttgaggg
      781 taatatcctt ctttttttag taattcgtag ataaatagac gccctttctg tgtccatttg
      841 gtattcatta caattttagt gccaccatcg gctttcggga tctcagttgt atgagatttt
      901 gtgtatcctt ggttcatgtg ttttttgcac aataaccatt ggttaccgac ttttttctga
      961 atacctagtt tatgaagtaa tttattcatc tgttgtggag acatcccata atctgctgca
     1021 atctgactaa ttgttactga atctgtagaa gataatatgc tatctaaata ggagatttta
     1081 ggttcgtact ctgcaatctt ttgttctgcg attaatcttc cagttctttc ttcttttagt
     1141 tgagttgcta attgaatgat tgtatctgga ttaagcaaag cttcttctac tttttctgga
     1201 gttagataac ctccatgttt tctaattgct ggcaacactt cacttgttac ccaacgttta
     1261 aattttttgg cagagggaag ttttgatttt aagattaaac tgtacaaacc agattcgttg
     1321 atgattgtca tctctcttga ttgacctgag gtcgtgattc gcgaccccat cttatcttcc
     1381 aaatctacat gccgcgacaa agcatcttta gtgtttgaat aacccaaaac actcgcaacg
     1441 tctttgccta caaaatatgg ttcatcattt actaaaattg tccgaacttc gttttgttcg
     1501 aaattaaaaa tttgcggtgt gttcatattt ctcattcctt tcttttatat tcgtaaacaa
     1561 atttaacaac ttttttctaa attcaattga cagtatctga gtaatactct atactaagag
     1621 cataattaaa taagacataa aaacattgat tttaaagctt tcttggcggt ctgcatttac
     1681 atatcaatag tgttttttgt tgtcttttta gttgttaaat ctgtttacaa gatatagtat
     1741 agctcattac tcagaattag tcaaattatt tctgcgtatt tttctaaact ttttttgtaa
     1801 acatttagaa aggttgtttt atcgatgaat acctatgaaa taataaaaga cttggcgaag
     1861 aggaaaaaga tatctatacg gcagttagaa attaattttg gatattctaa cggttatcta
     1921 gcaaagtgga aaaataatac tcctaattca gatgaattaa gtcggattgc tgactatttc
     1981 gatgtctctg tagactacct tctaggtaga gaagaaagag aaacccctaa acatgtggat
     2041 ttatcagaag acgatactgt tttttctttt gatggaaaag aaatatctaa ggagacaatg
     2101 cgtaaagcga ttgcaattgc taaagcttta gaggaaaatg aatagttgga gtgatggttt
     2161 gtatgtattt gaaattgaaa gaaatgttga gcgaatacaa tttgaaagta gtttatatgg
     2221 agatgaaaga gcctggtttc tactatccaa aacccagaat catttttttg aatgaaaatc
     2281 tatacggtga aactgcagaa gcctttcatt tatctcatga acttgcacat ttcagcgcct
     2341 ctcattttga attttcagtt ttgtacgata cttctacaac ttttcactca aaatttgaaa
     2401 cagaagcaga taaaatagct attctaattt tattaaatat ctatattgag aacgaactga
     2461 ctgataaatc tcagtttaat ctcgaaaaat ttatggagta ttattctatt cagaataagc
     2521 tcagatacac ttgttatgct gtctgccaat gctattttaa gaaaaaatat tcttacgcaa
     2581 ggcaatatgt atgaatacat caagaattat taaaaaaacc gatttcagca aaacattaaa
     2641 aaagcccgtg ctgcaacacg ggctctttcc tcatatatga gcttctacaa aaaaaatcat
     2701 atcacagaaa tgaggaagaa aaaatggaaa aagagatttt aataaaatct ccaggaaaa
//
LOCUS       DAXLPI010000185         2749 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXLPI010000185 DAXLPI010000000
VERSION     DAXLPI010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.191"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..962)
                     /locus_tag="ACF3T5_002646"
     CDS             complement(<1..962)
                     /locus_tag="ACF3T5_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3633159.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            1134..1268
                     /locus_tag="ACF3T5_002647"
     CDS             1134..1268
                     /locus_tag="ACF3T5_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633160.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(1261..1897)
                     /locus_tag="ACF3T5_002648"
                     /pseudo
     CDS             complement(1261..1897)
                     /locus_tag="ACF3T5_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2010..2645)
                     /locus_tag="ACF3T5_002649"
     CDS             complement(2010..2645)
                     /locus_tag="ACF3T5_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFD3633161.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(2679..>2749)
                     /locus_tag="ACF3T5_002650"
     CDS             complement(2679..>2749)
                     /locus_tag="ACF3T5_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFD3633162.1"
                     /translation="LLDEGIINQEEFDFKKKELLGL"
BASE COUNT          792 a          464 c          404 g         1089 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaaa taaagtgact
     1801 gcacaacgcc accaatataa ggaattgctc caataccgcc ttcaactatt aattcaagat
     1861 attctttttt ggttagtttt tcattgctat tatccatagt tacctcctat tttgtcattt
     1921 cctttacaag cattgtgact cctgatggca ctcttttgta taggttttat tattgtgcac
     1981 aatctaactc ttagtggggc gtggttcatt tattttttac ttactttact attaatcata
     2041 tcccattgag cataggattt ttctatgttc gatcctcttg aactaatttc tcctgtaaaa
     2101 gttactttat cgccctttgt aaaggtatca gcattgttta agtccttcgc aaaaattaca
     2161 tatgaaactt taggtgttgc agaaatatca ttccaacttt tatcatcgta ctctttgtct
     2221 gctataatgg ctattctagt agtcattgat tcgataacag ttccttcaaa agtatatgtt
     2281 ttaccactaa tatatttagt aaaagctgta ttttgctcag tagctggagt aatagaataa
     2341 taactatcta agaaagcacc ccagtcatga ttatttgtct ctaaaaattc ttcaaattga
     2401 gttaggcttt tattgctgct gttagatgta gtagtttgag tatcagatga tgagctttcc
     2461 tgtttgttat tgtcatttgc ctcgtttgat gaggaaagcg agaaacctac ggtagtaaaa
     2521 ataatcccga taataaggag aatcataggt gttttcttat ttgttttttt gataaatctt
     2581 acaattaaca tgattaaacc tataataaat aaaacaatac cagtaaaacc aaatataccg
     2641 tccattaata gatcctcttt ctcttttgat attgaatatt ataaaccaag caattctttt
     2701 ttcttaaaat cgaattcttc ttgattaata atcccttcat ctaacaact
//
LOCUS       DAXLPI010000186         2693 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXLPI010000186 DAXLPI010000000
VERSION     DAXLPI010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.192"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..679
                     /locus_tag="ACF3T5_002651"
     CDS             <1..679
                     /locus_tag="ACF3T5_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633163.1"
                     /translation="VVYGVAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQF
                     IRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYL
                     FEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTS
                     IHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPY
                     VLGKVVI"
     gene            723..992
                     /locus_tag="ACF3T5_002652"
     CDS             723..992
                     /locus_tag="ACF3T5_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633164.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1224..1520
                     /locus_tag="ACF3T5_002653"
     CDS             1224..1520
                     /locus_tag="ACF3T5_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633165.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1522..1944
                     /locus_tag="ACF3T5_002654"
     CDS             1522..1944
                     /locus_tag="ACF3T5_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633166.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1963..>2693
                     /gene="mobL"
                     /locus_tag="ACF3T5_002655"
     CDS             1963..>2693
                     /gene="mobL"
                     /locus_tag="ACF3T5_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HFD3633167.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNT"
BASE COUNT         1032 a          377 c          523 g          761 t
ORIGIN      
        1 ggtggtctat ggggtggcct attacttgtt aagtgagcca aaacagatcg cggcgtcaaa
       61 tattttcacg gcagtaatga cattaatgtg tgtcatgtgc ttttctattt ttagttactt
      121 actcgtccaa tttatcaggc aaaatttaac aatgagtaaa cttttcaaaa gtttattgga
      181 cttacttatc ttaggcagta tgttaacgac tctaacagat cggttcgaac aatctgaaaa
      241 aacggtagag gggataaaga acttcttaac gacgattgat caatttataa atggatatat
      301 gtatttgttt gaagtaggct ttattctcac tttgtttggt tgtgtattta taaaactcat
      361 taaaagatca atcgaaaaag aaatggttat tgatctaatt ccgacgctga gcggctatga
      421 gtattacgtt gattttggaa agtatgagga gttgatccat acggatatcg ttcacacgag
      481 tatccatgaa gtaaccgtta agggcatcag atctacttta aaagatggac aggagacgaa
      541 aacaatcgac cgaagaaaag aggtctacaa tttttataaa gttgaagcaa gtctacgata
      601 tggcattggg aaattgaggt tagatgggag taatattcaa caaaacaaac catatgtttt
      661 aggaaaagtg gtgatttaga acttcctatg aaagatgaat aaataaggag aatcttttga
      721 aaatgattct tctttacaga attgtgctta tagttggcac ggagttaaaa atcttaaaga
      781 aggagcagtt attcaaagtg aaagaacatc ataatcagtt actatgtatt aaaacgggta
      841 cgatgaacat tcaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
      901 cgattcgaga gcaacataag caagaaaaat taatggctac ttatagccaa ttgattgatg
      961 agtgggaaca attcaaggaa tctaaaacat aaaattccat ttaaacacct tattttttaa
     1021 agagcataag gaaacgtgac cccactttta aaaaaagtgg ttggataggc tgtgcctatc
     1081 acgtttctga aaaaaataaa tgaccccaaa ataccaatat ataggatagt acaaggatag
     1141 caaaggatag taaaaggata gaggtaggat agcgaaaagg ataggatcct tttcactatc
     1201 ctatattata ttggaggata tttatgaatg ttaaaattag agatttagat ccaaaattca
     1261 tttctgaaat tgatcgaaga tgcgttgaac taagtaatcg tacaggaaat aaatggagtc
     1321 gtaatgatta tttaaagcta ctcgttgaaa atgattttga tcgtcctttg atggaataca
     1381 aacaagaaaa gtttgaccag ttattagaaa agttttcaga aattcaaagt tataacacaa
     1441 agatattaga agaatatgtt agccaaaata atcgtattat agaaatcttg attgagcaaa
     1501 atagaggaag tgaactttga tatggcaaag gttatagaca aacacattta ctttcaccaa
     1561 acacatatcg atgtattgga tgaaactatt tctactcatg cagaggttaa aaactatagt
     1621 gaagctgttc gctttttatg tatgaactat gatccagaag tggagaaaaa agtaaattcc
     1681 aatcaagcaa agttgaatgg aatgagtaaa gaaatttcta ttttaaccga aatggtttca
     1741 gaatttgcgg atcttcattt gaaagataca ggaatattaa ttggtaaaaa ttcaactgtt
     1801 tatcaagaag caaaagaacg agtggaagga aaaattaaat ccaatcaaac aaaaaaatat
     1861 tcccctcagt tgaagaaacc tgtgaatgaa tctgttcatg ccagagtaga tccatttgat
     1921 ccaacgaaga ttctaaaaag ataaaaatga aggaggataa taatgactca atcgccttca
     1981 gttattctca cttgtcaatt tactttgccg aataaaaagt cattttctac ttatattgac
     2041 tatattacaa gggaacaggc tcttgaagaa aaggaacgac gtacgcccga agaagaacag
     2101 gaacttcaac gaatcaagaa tgctttagag gagttttata ttcctgaagg agaaactttt
     2161 tcggagagta agacgggtaa agaattcagt gaaacgtcga atgaagctca atcgttgatg
     2221 gaacatggaa tcaattttga caagttagag gaacaagatt ttaccaatta tctttcttat
     2281 atgacacgtc actatgcgtt agaacaaaag aaagaactga cgcaaagtga acaaaaagaa
     2341 taccaacgct tgaaccaagc aatgaaaaaa tatcaaacag aaccccataa aaaagcttca
     2401 ggaaatcttc ctggagtttt ttctatagct tcagacgaag tgaaaggaag tgatctgaag
     2461 gagattaagc aaatcttcca aagaggacaa caaaaaggct caatcattta tcaagatgtt
     2521 gtttctcatg ataattcgta tcttgaaaaa ataggattgt acgatcccaa aacagatact
     2581 ttagatgaag aagggttaaa agcagctgga agaaaaatga tggagacatt attcgagaaa
     2641 gaacaattga atgaaacagg ttactggatg gcaacgattc accgtaacac taa
//
LOCUS       DAXLPI010000187         2686 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXLPI010000187 DAXLPI010000000
VERSION     DAXLPI010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.193"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..205)
                     /locus_tag="ACF3T5_002656"
     CDS             complement(<1..205)
                     /locus_tag="ACF3T5_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFD3633168.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTL"
     gene            complement(202..855)
                     /locus_tag="ACF3T5_002657"
     CDS             complement(202..855)
                     /locus_tag="ACF3T5_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFD3633169.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1143..1663
                     /locus_tag="ACF3T5_002658"
                     /pseudo
     CDS             1143..1663
                     /locus_tag="ACF3T5_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(1661..2380)
                     /locus_tag="ACF3T5_002659"
                     /pseudo
     CDS             complement(1661..2380)
                     /locus_tag="ACF3T5_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010745868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
     gene            complement(2331..2540)
                     /locus_tag="ACF3T5_002660"
     CDS             complement(2331..2540)
                     /locus_tag="ACF3T5_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633170.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            complement(2522..2590)
                     /locus_tag="ACF3T5_002661"
                     /pseudo
     CDS             complement(2522..2590)
                     /locus_tag="ACF3T5_002661"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
BASE COUNT          839 a          571 c          395 g          881 t
ORIGIN      
        1 ccaaagtttg ttctatatat tttatttctt ttccattcat attactagaa atttcatttg
       61 ccatattatg aatcttttct tttctcgtct ccaaatatgt tttaggaaag ataaaaaaaa
      121 ttaatgaatg aactaatata attattattc caagaacaga aaatatttgt atgaacattt
      181 ttggaaatat ctttaatttt ttcattttct ctccaattta tatcctacat ttcttatagt
      241 agttatacag tctaattgta atttttttct aagttctttt atatatacat ctattactcg
      301 atcataagga gtttcttcgc tatctttcca tacataatca ataatttgca ttcttgttaa
      361 cacttgtcca ttattatcca ataaacattt taatacttct agttcttttg catttacatc
      421 tatttcttca ccatttattt tagctgtata gctattaaaa ttaaccgata gatccttata
      481 ttcaaacttc tctaaatgtc cataattctt cttaattaaa gaatctattc tagcttttaa
      541 gaccggcaat gaaaatggct tttctacata tccgtctact aaattagtaa atgcatcaat
      601 tttatattct tcatcactaa atgcagtcaa gattagaatt ggtaaattgc ttttctttct
      661 aatttctttc aacacttcta aaccatttat aaaaggtatc tgaatgtcta agataaccaa
      721 atttatatcg ctattaaatt ttgacaaggc ttctcttcca tctttagctt gaataacagt
      781 atatccaaat tctgaaagat attcacttat tccctctctt atcatattat catcttcaac
      841 agttaatatt ttcatatata cctccactta agcctataca ctagatatta tacagcatat
      901 tatggctcta taatatctag tgttggtgac gccttctgtt gccaatactt tttctttttt
      961 ttaataaatg caaacagaca ccttgaaatc ctttagaaaa aaggcttcta taatattttg
     1021 tgttgttgga aaatcgaagg cgattttcta ctaacacttt ttcttcgttt caactcaaaa
     1081 agagactcac ttttggtaaa atttagtcga ccaaaacatc accctaccaa aggagtcgct
     1141 ctatggataa tcatactaga aaattactcg gattaacaga taaacattta ttttttgatg
     1201 aagaatggct gattgaaaaa gaatataaag gcgttcaagc gcagtttatt aaagggagat
     1261 tggatgactc accttcccat tgtgaacact gtggatcgat tcgtattatc cgcaacggtt
     1321 cctatacgac acgtactcag atgttgaaag tcaaagaaaa gttgactatt ttagaattaa
     1381 aacgtacccg ttttctatgc tatgattgtg ggcagacctt ttctgcgaaa acggacttag
     1441 ttgatgaaca tcatcaacta acaaaggaac tgaagcaggc gattctcata gaactgtacg
     1501 aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt cggctcaagc gatattggaa
     1561 actcttactc aaagattcca cgacattgga gccgctcaaa cggcattatc atcgactgtt
     1621 taaacgacca attagtcaaa cagaaatcgt cgatgaaccg ttgtgcacaa tggtgtcctg
     1681 aacgtacagc gacaccttcc atatccagag cagtcgccac atcacgggga tggatgccat
     1741 ccaaattaaa agcaaccacg ccagtatgat gttcaggatc ttgcggtccg taaatggtga
     1801 taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac cagttcttgc tcataacgat
     1861 gaatattctc cataccaatc tcattcaagt aatcgattgc tcctgcaatg ttcggagtac
     1921 cagcttcaaa tttccatggc agctctttcc atgaactatc gtaaagattg acaaaatcta
     1981 tcatttcacc gccgaattcg actggctcca tttgttctaa caattgacgt ttgccatata
     2041 atacaccgat tcctgtcggc ccgcacattt tatgcccgct aaaggcgaaa aaatctgcat
     2101 ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg ggcaccgact ggactatcct
     2161 caactacaac agcctgttca accctcagcc gtttcaagca caacaaataa ccttctggat
     2221 ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga atcaattttt tccttaatca
     2281 tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc agaattgaga ctactccgat
     2341 tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat ccactggtct cccaaccgct
     2401 tgacttatcc cttcataaaa ggacagttct gctgccgtcc caacaccttg ttgatagtct
     2461 gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg cttcagtaaa tagtgatgaa
     2521 tactctcgta aaacatccat tcactgtcaa tcatcgtccc atcaaaatca aaaataagtg
     2581 ctttggtcat cttctttccc ccttacccaa aacaagcaaa agctgtgtcc tgctctctct
     2641 acagtatcag cataaaaact gccgtctgaa aagtcaaagg tgaaag
//
LOCUS       DAXLPI010000188         2663 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXLPI010000188 DAXLPI010000000
VERSION     DAXLPI010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.194"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            2..793
                     /locus_tag="ACF3T5_002662"
     CDS             2..793
                     /locus_tag="ACF3T5_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFD3633171.1"
                     /translation="MPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFDGFSP
                     FPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYFGSIL
                     GINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISFVLSM
                     IGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILLQLSF
                     IGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            863..1603
                     /locus_tag="ACF3T5_002663"
     CDS             863..1603
                     /locus_tag="ACF3T5_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFD3633172.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            1614..2618
                     /locus_tag="ACF3T5_002664"
     CDS             1614..2618
                     /locus_tag="ACF3T5_002664"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFD3633173.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT          839 a          438 c          544 g          842 t
ORIGIN      
        1 aatacctatt tctgaatatc ggaaaaaagc acgagtttct ttggaaaatc aatggggaat
       61 caatacttgg cttgttttct tatctataat atttacgagc gtcatccaga acttttttga
      121 cggtttcagt ccttttccgg aagaaagcat gcagcagcac ttattgagtt ttctagtcga
      181 taatttactt ttattcgcat tcaactatgc attgttttac attgcgctag tagttgtacg
      241 gggtggcaga ccaaaaagta accttttatt ttcagttttc caaagaaagt attttggttc
      301 gatacttggc atcaatttga tcaattcagt catcaactgg ctactcaaca gtttaatctt
      361 tctgcccatg tttttaatca gcggagtgag tacttatatg cagctgcttt tcagttcaaa
      421 tgcggtacct acgggtgtca tgccagaaga ttttcttgat atcagctttg ttttatcgat
      481 gatagggatg atttttgttt cagcactcat catgagtatt gttggcggtt tattccaatt
      541 tgctgcatgg acaaagttcg actatcctga tttgtcggtt attcaatgtt tgaaatatgg
      601 ctggttttta ttgaaagata gaattggtac gtatattttg ttgcagctgt cttttatcgg
      661 ctggtatttg ctaggagcaa tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt
      721 gaatgtgtca gttgcagaat tttatgaaca agcacggatt gaaaaaggag gtccagacgc
      781 ctattttact tagcatgtaa gcagtttatt catcaaaaaa ataacttttg tggtaggata
      841 aatctttgag ggggaatgta cgatgaaatt aactgtttta ggatgcttag gagcgtatcc
      901 ttataaaaat caagggacaa caggttattt gcttcaatct gacgggttca atttgttgat
      961 cgatgcaggt agtgctacgc tgatcaaatt acaagaacac cttgatccac ttgatctaga
     1021 tgctgtgatc cttagccatt atcatcatga tcatattgct gaccttggtg tattacagta
     1081 ttactggcaa cttcatccta agagaaatcc ggagacattg ctttcaattt atggacacac
     1141 aaaagattcc attcactttg aagaattgac gttggaaaat gtctcaaaag gacatcctta
     1201 ttttgaagct gaagaattaa aaattggtcc ttttctgatt acttttatgg aaacaatcca
     1261 tccagtagta tgttacgcca tgcgtatcgt agaagaaaca acggggaaag tattcgtatt
     1321 tacaggtgat tcaggttatt tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt
     1381 tctagcagat acctatcttt ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa
     1441 agaagctgga gaacttgcga aatcagctaa cgtaaagaaa cttgtactta cacacctacc
     1501 tcagtttggt gatttagaac aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat
     1561 tgatatagaa cttgctgcag ttgacaaagt gtttaacatt taggaggaag aacatgattt
     1621 ttgtaccaaa tgaaaaccac gacccaagaa tcaatttagc aattgaacaa tatttgctgc
     1681 aagaaatgcc tttagatgaa cctattttgc tcttttatat caatgaacct tcaatcatca
     1741 tcggcagaaa ccaaaatacg cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca
     1801 ttcatgttgt ccgtcgctta agtggaggcg gagctgtcta ccatgatttt ggaaacctga
     1861 actttagttt tatcatgcct gatgatgggg attcattccg tgactttgcg aaagtaacac
     1921 agccaatcat ccaagcactt catgaattgg gtgttgctgg ggcggaattg aaaggccgaa
     1981 atgatctagt cattgatggc atgaaatttt ctggcaacgc gatgtatgct accaacggtc
     2041 ggatgtttgc tcatggaaca attatgttcg actctgatat caatgaagta gtcaatgcat
     2101 taaaagtaaa aaaagacaaa atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa
     2161 cgaatatcaa accatttctt ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc
     2221 aagaaatctt gttgaaaatt ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat
     2281 tgactgatga agattggaaa aaaatcaatc gaatctctga ggaatattat cgaaattggg
     2341 attggaatta cgggaagtct ccagatttca atttctctcg ccaaaaacgt ttttctatcg
     2401 gttcgattga agtccatttg aatgtctcag aaggaatgat caaagaagca aaaatctttg
     2461 gcgatttctt cggactagga gatatctctg acgtggaaaa aaaattggtt ggacagaaat
     2521 atgataaagc atctctatca aaaatagtca gccaaattga tgtgaagaaa tattttggtg
     2581 cgatagaacc agaagaattg cttaccttac tttattgaaa ataaaaaata gcggaatcca
     2641 catatatgga ttccgctatt ttt
//
LOCUS       DAXLPI010000189         2609 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXLPI010000189 DAXLPI010000000
VERSION     DAXLPI010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.196"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..1152
                     /locus_tag="ACF3T5_002665"
     CDS             <1..1152
                     /locus_tag="ACF3T5_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HFD3633174.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="ACF3T5_002666"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="ACF3T5_002666"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFD3633175.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          865 a          493 c          624 g          627 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgt
//
LOCUS       DAXLPI010000190         2561 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXLPI010000190 DAXLPI010000000
VERSION     DAXLPI010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.198"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            46..657
                     /locus_tag="ACF3T5_002667"
     CDS             46..657
                     /locus_tag="ACF3T5_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFD3633176.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            730..1659
                     /locus_tag="ACF3T5_002668"
     CDS             730..1659
                     /locus_tag="ACF3T5_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HFD3633177.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1796..2554
                     /locus_tag="ACF3T5_002669"
                     /pseudo
     CDS             1796..2554
                     /locus_tag="ACF3T5_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
BASE COUNT          894 a          358 c          478 g          831 t
ORIGIN      
        1 atatgaagtt ataaaattta atttatattg gaaggaggaa aataaatgta ttttatacta
       61 cctacacttt atagcctttt atgtgtaatg gcaccatgta ttatttatat ggtatttttt
      121 tgcaaaaaaa ataccctgaa acacaatatc ctgattttga tttttatgct gtactgttgg
      181 aaagtttttg aattgactgg tacaggtgga ctaacagata ttttttatag acctgataca
      241 ggtaggaaat tttctctagt acagggcagt gttaatcttg taccattcag tggactaaac
      301 attagcttta tattaaatat agtaatgtgt gtgcccttag gattcttact accaatcata
      361 tggaaaagtt ataggaaaat agttcctgtt ttcttggctg gggctctatt ttcactaatt
      421 attgaagtct cacaaattat aactacaaga gcaacagata ttgatgattt aatagcaaat
      481 acctttggtg cggttatagg ctttatactg tgggctgttt taagtagagt ggtgtccttg
      541 aaggacaagt catctacagt agattattta gaaccttata tttatatatt gattggtttt
      601 accggaaaat tcttactgta ttttcctttc tggtttgctt ttgaagtatt atcctgataa
      661 ttccatagaa aataatgagt ttgagttgag aaagaatatt cttgtacaca actctggaaa
      721 ggaggaagta tgaaaagatt cttaaaatgg gggcttgttc ttttagcgtc tttgattgtg
      781 attctaatgt gtacagcggt tgttatttct tatgtgaatc ggccgtatgc aagagctgtt
      841 tatgatagga ttaaacgcat tcttgacaaa gatttttatg accatctata tctaggttct
      901 tcatttgaaa taataaacct tgatacaatg gatattcata gctatatgaa aaaagaccaa
      961 gaagaattac ccgcctcatt gagcaaatta tttacgattg attatgcgct tagtgtccta
     1021 agtttagatg aacagattac aattagtcct actgcacttg acatgcagaa agctgactca
     1081 tctactgcac atcttcaaga aggagatatt ttatctgttt atgacattat ctgtgctatg
     1141 gtcttaccct caggaaatga tgcagcttat agtttggcaa tttatacagg tggaaaactt
     1201 gatcctgatg caaagaatga tgaagaacgc tatcaaagct tcatgaaaaa cttagatcgt
     1261 tatctaagtg agcaaggcta taaacatact cattttactg acgctgctgg ctatgactcc
     1321 aatgctacaa catcagcgga agatttgaca aaagtagctg caaagctact aaagaatgat
     1381 aaatttgcag aaatagttgc caaggaaact tatagagcaa aatcaattaa gggaaatgtt
     1441 tataactgga atactacgaa tcaatttatg caacaagata atagatactt tactgaaggt
     1501 gtaaaaggtg taaaaactgg atcgtttgat gggagataca atctatgtgt gtattataca
     1561 tctagttctg ataaccacta tttacttgta agttttggtt cagagagtga tagtaggcgt
     1621 tatgtagatg ttcaaaatct attgcataat ctgccttgaa atttcgtgcc tttaagcgcg
     1681 tatctaaagt ttctttgaaa agtttgagaa tgaagaaagt gagcagtcga aaaacggttg
     1741 ctcacttttt tgccctaaaa tcgaaaaaat ataaaaaagt cctttacaac acgtctcagg
     1801 aaaaactgct aagtcaattc taatatcttg tggagcaaga cttgcacctt ttgatataag
     1861 agagcttaga gaactaatga gcgaagatga actagaactc gataaaattg gagatagaaa
     1921 aactgccttg tttgtaataa tatcagatac agatgataca tttaattttg tagtttcaat
     1981 aatgtattct caattattta atctactatg tgataaggca gatgatgtgt atggtggaag
     2041 acttccagtt catgttaggt gtctacttga tgagtttgca aatatcggtt tgattcctaa
     2101 atttgaaaaa ctaatagcga caattcgttc aagagaaata tcggcaagta taatactgca
     2161 agcacaatct caattaaaag caatttataa agaccatgca gatacaatag ttggtaactg
     2221 tgactctaca ctttttctag gaggaaaaga aaaaacaaca gtaaaagaat tatcagaaac
     2281 cttaggaaaa gaaaccatag acctatataa cacatcagaa acaagatcaa atcaaaaatc
     2341 ttttggtcta aattatcaaa agactggcaa ggaacttatg agtcaagatg aaataactgt
     2401 aatggatggt ggtaagtgta tttaccagct aagaggagca agaccttttc tatctgataa
     2461 atttgatatt acaaagcata agaattacaa attgttggaa gattatgata agaaaaactt
     2521 gtttgatgta gaagagtatt taaaacaaag atagcgtaaa a
//
LOCUS       DAXLPI010000191         2509 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXLPI010000191 DAXLPI010000000
VERSION     DAXLPI010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.200"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(489..614)
                     /locus_tag="ACF3T5_002670"
     CDS             complement(489..614)
                     /locus_tag="ACF3T5_002670"
                     /inference="COORDINATES: protein motif:HMM:NF019687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633178.1"
                     /translation="MLFPPFKSSKSRKNPSDKYSYHWDFNTKIAVYYTLIATDAL"
     gene            599..>2509
                     /gene="tet(S)"
                     /locus_tag="ACF3T5_002671"
     CDS             599..>2509
                     /gene="tet(S)"
                     /locus_tag="ACF3T5_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HFD3633179.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVR"
BASE COUNT          900 a          372 c          441 g          796 t
ORIGIN      
        1 atgatatttt ccaagtcaaa cttaatatta acaattcaaa actattggaa ccactgcaaa
       61 atattttgtt aagcagtata tatttcaaaa attatacgta atatatttgt agaaaggttg
      121 tggacagttg tcctattgac ctgaaaagta atataatagc tggatgaata aagaatccct
      181 tatgaacttg taaatcgtta ttaaatcgct gaattttgat agccattctg aagagttatc
      241 ttcaactact acactaactt ccattaattt ctcattaatt tccctttcgt tcaaaaaata
      301 tggttggctt tacaagcatt ctatcatatg atagaattaa gataacattc aaaaggagat
      361 aataaaaatg cggcaaggta ttcttaaata aactgtcaat tagatagcgg gaataattaa
      421 ttaagcatcc ttatttaaaa taaggactgg ttttttgtgc ccagtttaag aatactttta
      481 tcattttatc ataaagcatc tgtggctatc agtgtgtagt atacagctat ttttgtatta
      541 aaatcccagt gataagagta tttatcactg ggattttttc tgctttttga gcttttgaat
      601 ggaggaaata aaattgaaaa ttattaatat cggtatctta gcacatgttg atgcaggaaa
      661 aactactttg acagaaagct tactatacag tagcggagca attaaagagt taggaagtgt
      721 agatagcggt acaacgaaaa cggatactat gtttttggaa cgccagagag gtattactat
      781 tcagaccgca ataacatctt ttcaacggga aaatgttaaa gtaaatattg tagatactcc
      841 tggacacatg gattttttgg cagatgtata ccgttcatta tctgttttgg atggagctat
      901 tttgctaatc tctgcaaaag atggagtaca gtcacaaact cgtatactat tccatgcact
      961 tagaaagatg aacataccta taatattttt tattaacaaa attgatcaaa atggaataaa
     1021 tttgccagat gtttatcaag atattaagga caaactttct gacgacatca taattaagca
     1081 gactgtgaat ctaaatttga aaccttatgt aatagattat actgaaccag aacaatggga
     1141 gacagtaatt gtgggaaatg attatttatt agaaaaatat accattggga aaacattgaa
     1201 tattgcagaa cttgaaaagg aggaaaacga aagaattcaa agttgctcct tatatcctgt
     1261 ttatcacgga agtgcaaaga ataatattgg aattaaacaa cttatagagg taattactag
     1321 caaattattt tcacccacac aactcaattc agataaactt tgtggaaatg tttttaaagt
     1381 agaatattca gatgatggtc aacggcttgt ctatgtacgt ctttatagtg gaacgctaca
     1441 tttgcgagac tcagtcaata tatcagaaaa ggaaaaaata aaagttacag aaatgtatac
     1501 ttcaataaat ggagaattac gccagataga taaggcagag cctggtgaga ttattatttt
     1561 aaaaaatgag cttttaaaac taaataacgt acttggagat aaaaaaagat taccacatag
     1621 agaaattctt gagaatcctc ttcctatgtt acaaacaaca attgaaccat gtaaatcagt
     1681 acaaagagaa aagttactag atgcactttt tgaaatatcc gatagtgatc cccttctaca
     1741 atattatgta gatacagtaa ctcacgaaat tgtgctatct tttttaggtg aggtccaaat
     1801 ggaggtaact tgtactctga ttcaagaaaa atatcatatt gagatagaaa caagaaaacc
     1861 aactgtcatt tatatggaaa gaccattaaa aaaatctgaa tttaccattg atatcgaagt
     1921 acctccaaat cctttctggg cttctattgg tttatctgta acaccacttc ctttgggtag
     1981 tggcattcag tatgagagcc tggtttctct aggttattta aatcaatcat ttcaaaatgc
     2041 agttatggaa ggtatacgct atgggtgtga acaaggattg tacggttgga aattaacaga
     2101 ctgtaagatc tgttttaagt atggtctata ttacagccct gtcagtacgc cagcagattt
     2161 ccgaatgctt gcgcctattg tactagagca ggcttttaga aagagtggta cagagttatt
     2221 agagccatat cttagcttcg aaatttatgt accacaagaa tatctttcga gagcatataa
     2281 tgatgcttcc aaatattgtg caaatatttt aaatactaag ttaaaaggta acgaggtcat
     2341 tctcattggt gaaattccag cccgttgtat tcaagagtat cgaaacagtt taactttctt
     2401 tacaaatgga cgcagtgtct gtttaacaga gttaaaaggt tatcaggtta ctaacattaa
     2461 gtctgctttc caaccacgtc gtccaaataa tagaatagac aaagtaagg
//
LOCUS       DAXLPI010000192         2506 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXLPI010000192 DAXLPI010000000
VERSION     DAXLPI010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.201"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            32..1156
                     /locus_tag="ACF3T5_002672"
                     /pseudo
     CDS             32..1156
                     /locus_tag="ACF3T5_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1576..2220
                     /locus_tag="ACF3T5_002673"
     CDS             1576..2220
                     /locus_tag="ACF3T5_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3633180.1"
                     /translation="MYKTANELFNRLKSEKKELILNYILEEFGRVGYESASVRKIADK
                     ADLSAGSLYQYFNNKEGMLRVAIEHVFVILDEYINNINNAPLKLENRLEVMFETILEL
                     GRKHPELIVFYNKIPGDEQMTSEWVKQFYNNTSFFKVYWHTVNELQDEKEISDKIDPV
                     VYTFIIDTMMLSGELLQNTQYQEIKAKIFLEDSIKSSLILKENVIETLTVLARG"
     gene            2227..>2506
                     /locus_tag="ACF3T5_002674"
     CDS             2227..>2506
                     /locus_tag="ACF3T5_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3633181.1"
                     /translation="MTISNKNSMREEIIIIAIKHFSKFGYEKTRLLDIANEAKTSTTT
                     VYSFFKTKHDLYEAAVEYDLNIINLELEKIVKESSTLDEYIINIVERVR"
BASE COUNT          949 a          351 c          475 g          731 t
ORIGIN      
        1 aatataagaa aacacactgg aggaattgtt aatgtcagaa agaaaaccac gtcgaacgtt
       61 tactaaagag tttaaaaatc aaatggttca gttatatgcg tcagggaaaa agagaaccga
      121 tattatcaga gagtatgatt taaccccgtc tacttttgat aaatgggtga aacaagcaaa
      181 tacctctggc tcatttaaag aaaaagataa cttaacacct gaacaaaaag agttaattca
      241 attgcgaaag caaaataaac aactcttgat agaaaatgat attttaaagc aagcggcgct
      301 gatattcgga cgaaaagaca agtaattgat gcaaataagc acaaatactc gatatcagcc
      361 atgtgtaaag tactgggtat tgcaagagcc acctattatt atcgagaaaa agaaacgact
      421 caaggcgcag tagaagcaaa attagaagaa gcggtggaag aagtttttca acaaaataga
      481 cgagcatatg gttcacgcag aatcaagaaa cgccttgata agaaaaatct cattttgagt
      541 cgtcgaaaaa ttcgtcgcat catgaaaaga cgaggattga aatccagtta cacgaagccc
      601 aaatatcgcg tacattcgtg ccaaagcaat caaaagaaaa ttgcgaatca cttagatcga
      661 aagtttactc aaacaaaagc attgtcagcg gttgtcacgg atttaactta tgttcgggtc
      721 gggacaaatt gggcttatat ttgtttgatg attgatttat tcaatcgtga aattattggc
      781 cattcttgcg gaaataaaaa agatgcgcag cttgtaaaaa gggccattca aagcattcct
      841 tactcacttc aagaaatcga actcttccat acggatcgtg gaaaagaatt tgataatgaa
      901 ttgattgatc ttatcttaga aacatttggc attcaacgct ccttgagtaa gcctggatgt
      961 ccatatgata atgccgtttc agaagcgact tacaaagctt ttaagacgga atttgtgagt
     1021 caatatcaat ttgaaacgat ggcacactta gaattagaat gttttgatta tgtcaattgg
     1081 tggaatcatc atcggatcca tagttctctt ggttataaaa caccaattga ggtacgctat
     1141 gcggaggttg gcgtagcgaa cccttgataa tgagcaagaa tcggttacct tctgttcgag
     1201 cggcttattt tattcaaagt gagcacgccg ctaagaaaac cgatttgagg aggctcgttg
     1261 tcaagggcaa ctggatatgg tgtcataaac agaccttata atttttgtct agaaaagtgt
     1321 tgccattcca tacatctaga gttattttat atccccttga aagtcaggag tcttgtaaag
     1381 aataatgaaa atattaagac ttctgaaatt aagtcgggtc acacttctgt aaatagttca
     1441 gacgaaatta ttagtagtag aaaagtaaat ggttggaaaa tgattcaatt aggagaagct
     1501 ttaaaaaatg agcggtcgtt tttataaggt taatatggta tacttagtta taatatcttt
     1561 tttgaggaga tagctatgta taaaacagca aatgaattat ttaatcgatt aaaatcagaa
     1621 aaaaaagaac tcattttaaa ctatatctta gaagaatttg gaagagttgg ttatgaatct
     1681 gcttcagttc gtaagattgc tgataaggca gatttaagtg caggatcatt atatcagtac
     1741 tttaacaata aagaaggaat gctaagagta gcaatagaac acgtttttgt tattttagat
     1801 gaatatatta ataatataaa caatgctcct ttaaaattag aaaatcgttt agaagtaatg
     1861 ttcgaaacaa tcttagaatt aggaagaaaa catcctgaat tgattgtttt ttataataaa
     1921 attcctggtg acgaacaaat gacaagcgag tgggttaaac aattttacaa taacactagt
     1981 tttttcaaag tatattggca taccgtaaat gaattacaag atgaaaaaga aataagtgat
     2041 aaaattgatc cagttgtcta cacgttcatt attgatacga tgatgttatc cggagaatta
     2101 cttcaaaata cgcaatatca agaaataaaa gccaaaattt ttctagaaga ctcaataaaa
     2161 agttcattaa tattaaaaga aaatgttatc gagacgctaa ctgtacttgc aagggggtaa
     2221 gagtctatga caataagtaa taagaatagc atgcgagaag aaattataat tattgctata
     2281 aagcattttt ctaaatttgg ttatgagaaa acaagacttt tagatattgc taatgaagct
     2341 aaaactagta ctactactgt atatagtttt tttaaaacaa aacatgatct gtatgaagct
     2401 gcagtggagt atgatttaaa tataataaac cttgaacttg aaaaaattgt taaggaaagt
     2461 tctacactcg atgaatatat tataaatata gttgaaagag tacgat
//
LOCUS       DAXLPI010000193         2485 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXLPI010000193 DAXLPI010000000
VERSION     DAXLPI010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.202"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..301)
                     /locus_tag="ACF3T5_002675"
     CDS             complement(<1..301)
                     /locus_tag="ACF3T5_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFD3633182.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            394..743
                     /locus_tag="ACF3T5_002676"
                     /pseudo
     CDS             394..743
                     /locus_tag="ACF3T5_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1015..1305)
                     /locus_tag="ACF3T5_002677"
     CDS             complement(1015..1305)
                     /locus_tag="ACF3T5_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633183.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2205)
                     /locus_tag="ACF3T5_002678"
     CDS             complement(1513..2205)
                     /locus_tag="ACF3T5_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFD3633184.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          734 a          459 c          349 g          943 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgagttt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2341 ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat
     2401 ttatggtgaa caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag
     2461 attactgtat ttattacaaa aaacg
//
LOCUS       DAXLPI010000194         2474 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXLPI010000194 DAXLPI010000000
VERSION     DAXLPI010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.203"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(116..469)
                     /locus_tag="ACF3T5_002679"
                     /pseudo
     CDS             complement(116..469)
                     /locus_tag="ACF3T5_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(582..839)
                     /locus_tag="ACF3T5_002680"
     CDS             complement(582..839)
                     /locus_tag="ACF3T5_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633185.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(1436..1714)
                     /locus_tag="ACF3T5_002681"
     CDS             complement(1436..1714)
                     /locus_tag="ACF3T5_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633186.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(1748..1999)
                     /locus_tag="ACF3T5_002682"
     CDS             complement(1748..1999)
                     /locus_tag="ACF3T5_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633187.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT          777 a          492 c          329 g          876 t
ORIGIN      
        1 aatcatttgt ttaaaatttt cttcatatcg ggtcataaaa attcatcctc tcgtagataa
       61 gtttaattta ttcttgtctc tacgaaaagc tgtctaagat ttgatactaa caccaatcgt
      121 ccccaaaagg tagatcttct ctaccactag ctaatctact tctccatctt cgttctcttt
      181 tcatagaacc tgcgctgaac tcacacccgt taaactcagc tatgacagta tcttcaatta
      241 tgttaatctg tccatctagt tcgtattcaa tcctaaatgg aaattctcca taggtgattt
      301 ccggttcggg tattttaggt gagattaatg caaagaaagg aaatattaag cttggcaaca
      361 tcaaaaaaac tcctcctatg atcacaattc ccgatgtaat ccaaaatata atttgtacaa
      421 ttctcattga tccattgtat tccattttat tccccctatt actattcata tccgcctatc
      481 tatatcgatc ttccaatgca gagataattg cttccatacc ttgatttttt actgagttac
      541 ctatgcgaga tatcattctc cactaaggtg catcatattg tctatctaac atgattaatt
      601 ctccaatagg tgtatactcc tccaaaaaca acatttaatg catctccctc gacaacataa
      661 gctaccatgt ttgctgtata tgtgtttgag tctaattcat aagcagataa atttactgtc
      721 gctggattaa atataatcgc attcgtattt atagctactg aatttgctgc ttcagcacca
      781 ccttttgaat gtccgatcat tgtgatttca atagtaggat ttacgtggac agccctcacc
      841 gtttaccctg accattatag aaatcgctta cttttttgaa ctacgcaaca aaggatacaa
      901 tgctcctaac ttcactgtct ccatttttgt ccactaagga atataaaaaa taggcattaa
      961 ttcatttaat tcggtcacaa cacgttacct tttaaatcat tatactgtat ctcatacatt
     1021 attatcaata aactattata aaaatgcaaa aaatgcttta tcaatctatt ttgtaattgg
     1081 tcataatatc attgaaatca taccctgtat cgatttttcc tatgaatctc gtctatttgt
     1141 gattgacgaa aaagaggcaa gacagacact ggaatggtaa ttttacccac tattttgctc
     1201 caactatttg taatcgtacc cacttttgaa ataccgagat gatcatttta attgatatgc
     1261 tgattcccaa gcaaaacccc aagaaattaa atgctctatc gcatctagct tcttgggttt
     1321 tgcttatata aattccaaca tttaaatttt ttatgctgtt cggtctcaga aatgccgttt
     1381 ttgcttatat cacaatatca agatgtcaaa ttattcgtgt tgccgatgac tttgattaat
     1441 ttttttcaaa ttgtttatta agactaatgc tgtctttttg cagataccaa tgttcgaact
     1501 ttttaacatt atcatatgat cctgcaagtt ttaacttctt accattagat gttatcaaag
     1561 ttataattaa ccttgccttt ttcccattag gatcttttat tttatgaatt ttctgcaggt
     1621 aatctctcct aatggcagct atttttacct catcagtgct gataaaccat tgatcatcca
     1681 catacatact gcctgttatg ttgtgttttt ccatttcttc cgagaacgaa aagtttaagg
     1741 ctcgttctta cttccaaagt tgaatgagtc catggattgg acttagtgtg agaattgaga
     1801 acaacaataa gaatccatat cctcccgcaa acatccctaa ggctatactg tctctccatc
     1861 cataagtagg attagaagag aaaagagtca ccgtttgtaa taatatgaaa aggaataatc
     1921 cagcacaaac accaaagata acaactccaa acattgaatt tttatacaat gaccatttat
     1981 ttataggctt ttctttcatt ttacttcccc ctactgttct gttcacacgg aatgaattca
     2041 aagttcataa gccctcctct ttgaacatat gaatacgact tgctaactat actttaaaaa
     2101 agcttgtttt aagcggttat aggcgagttg taatctctat tcatctcatt agcaaataac
     2161 ttgccgaaaa gtctactgaa attaaagcag atagtggaag aattatttag ccaggataga
     2221 cactatttgt gaaacacatg tctttacttt tgaaattcta tgatgaaaag ccttggaata
     2281 cagtagctct taattggtaa tactttttcc attataaata acctcttttc attttatatt
     2341 tatcccctct aaaaataata tctaatctaa tattttaaac ttatatttcc ctcgctgtgc
     2401 taatagagca ttactaaata aatagaaact agaaaaaagc attacgcctg ccagaataaa
     2461 ccaattattt gtat
//
LOCUS       DAXLPI010000195         2452 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXLPI010000195 DAXLPI010000000
VERSION     DAXLPI010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.204"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            81..968
                     /locus_tag="ACF3T5_002683"
     CDS             81..968
                     /locus_tag="ACF3T5_002683"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFD3633188.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            1054..1284
                     /locus_tag="ACF3T5_002684"
     CDS             1054..1284
                     /locus_tag="ACF3T5_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFD3633189.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(1341..2306)
                     /locus_tag="ACF3T5_002685"
     CDS             complement(1341..2306)
                     /locus_tag="ACF3T5_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFD3633190.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT          821 a          397 c          486 g          748 t
ORIGIN      
        1 cattttttat gaaaaaatcc ttgtttctag cgtatactag catagaagca aggatttttg
       61 atttttagga ggtcgaaaaa atgaaacttg gttttatcgg aacaggcgtc atgggaagtg
      121 ctgtcgcacg ccatctacta gaagcaggac acgaagtagc tgtctataat cggacaaaag
      181 caaaagctga tcctttagta acagaaggag ctatctgggc agatacacca aaagcggtag
      241 cagaacaaag taatattctc tttacaatgg taggttaccc caaagatgta gaagaaatct
      301 actatggaca gtcggggatt ttttctgcag atatctcagg acatatttta gttgatttga
      361 ctacaagtac accaagcctg gcagaaaaaa ttgcaaaaac agcaaaagaa aaaggcgcag
      421 atgcgttaga tgcaccggta tccggtggtg acttaggagc aaagaatggt acactgacga
      481 tcatggtcgg tggagaagaa gccgtttatg atcaagtact gcctttattc aaagaatttg
      541 gtacgacatt tacattgcat ggttctgctg ggaaaggtca gcatacaaaa atggctaatc
      601 agatcatgat tgcaggtacg atgacaggaa tgacggaaat gcttgtctat gcacaaaaaa
      661 acggacttga tttaaagaaa gtgattgaga cgctttcagg tggaagtgca gctaactggt
      721 ctttaagcaa ttattctcct cgtattttaa aagaagatta cacacctggt ttttttgtca
      781 aacattttat caaagattta aagattgcat tagaagaagc agaaaaaatg gatcttgtat
      841 tgccagcaac tacccaagca ttgaaactat atgaagagtt ggcagataaa ggttttgaga
      901 atgacggaac ccaagctttg atcaaacttt ggtggcctga aggaaaaata ccagaaaaaa
      961 aatcataaaa aacgagtttt tttagagaaa atcaatgcaa gtaggctatg cttttgatac
     1021 aatataacag aaataagaaa aggaggacct ccgatgacac attcacaatt agtcgcaatc
     1081 ataaaacgac tggaggctat ggtcgagtca gcagataatg aactgcaagt tcgccgtttt
     1141 gaaaaagaag gcgtagagaa gtgtacagta acttatgata aagcgaccga tacatttgaa
     1201 ttaactgaaa cagatactca tcagcaatac gaatttgata atatcgatat cgtagcgatg
     1261 gaaatctatg acttaatcca ataaatttta ggataattga catttgaagt tgaagatgcc
     1321 taaaagaagc cgagaagcaa ctaatttagt tgcttttcag cttctttttt gtttaacagt
     1381 tcaatttcaa actgttcttt ttctaatttt ttgcggtaac tgatcaagat ttgtacagga
     1441 tcgatttctt cttttgacaa ttgacgtttc aaagataaat gagcttcttt cttttcataa
     1501 tagaagtcag gagaaacgtc accagaggta atcattgttt ctgcaagttg accataacaa
     1561 acgaggtagt gctttttctg actctcatct gctgctaatg gcatgctgaa ggtgagcgat
     1621 ggtgttctta aaaactgctt ttgttcctgg atccgtctaa taaaataagt aactaattcg
     1681 atatcttttt gaagcatacc ggctttttcg aaattcaact gatagatgaa atgttgttgt
     1741 ctttttttca agtaagtcag tagcttttta tttcggcctt gaaacagccc ttggagttcc
     1801 ttttttttct gttcaaaagt catttcctgt aggtcaggaa gctggactcg caaagctaac
     1861 aaactaattt cagacaacca aggcatttga taagtttctt ccattatacg caaaatagac
     1921 ggcatttttt tatagagacg aaaaggcccg tatgttcgag ccgtaggagt atctgttaat
     1981 acaaatccct tattggtaat atgtacataa ttataattac tgaaataatt catttgtcgg
     2041 ttataaaaag gacggtaatg ttggatcaat tgacactcga gcaataaagc atctaattcc
     2101 gtgtctacta cgacgaaatc aaaatctgtt atatgatgaa tcatacgtaa tacttttttt
     2161 gaatgctgtt tattctgatg aaaatagctg cttactctat ttttgagccg ttttgcttta
     2221 ccaacataga tgaccttacc atctcggtct ttcataagat agacacccgg cagcatgggg
     2281 agagttttca ttttttcttt taacatagga tctcctttta cgctttgaat aaaaagacta
     2341 taacaaattt tgaaataatg gacaagtttt agggaattct atagttctgt tcacagaaaa
     2401 tactttgata atcaaaatat gtgagataga tgatttttct aataaaagaa at
//
LOCUS       DAXLPI010000196         2418 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXLPI010000196 DAXLPI010000000
VERSION     DAXLPI010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.205"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(441..944)
                     /locus_tag="ACF3T5_002686"
     CDS             complement(441..944)
                     /locus_tag="ACF3T5_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFD3633191.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1303..2328
                     /locus_tag="ACF3T5_002687"
     CDS             1303..2328
                     /locus_tag="ACF3T5_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFD3633192.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
BASE COUNT          768 a          379 c          459 g          812 t
ORIGIN      
        1 tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag gatcaggcag
       61 aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc atagaaaata
      121 taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg agtagggtat
      181 aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg ttaagatttt
      241 aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag cctgtcagtt
      301 tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa atagattttt
      361 ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt gtctattacg
      421 gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa taaaaaagct
      481 acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc taacttattt
      541 gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc tcctgttgta
      601 ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg aatcaattct
      661 gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag tctcttgaaa
      721 ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt ggtctcacgt
      781 aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac attgctgtaa
      841 gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa ccataataga
      901 aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat ctcctccatt
      961 taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag tttaaggacc
     1021 aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg tttctttaac
     1081 aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca aaacgactcg
     1141 gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac taagtttatg
     1201 aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg gcatttcatc
     1261 ttggttcagc tttttttcca attactagga aggagggata gattgttcaa gacttatttt
     1321 cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct acttatcttt
     1381 attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt ttcatcatta
     1441 gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct gaaacgtatg
     1501 tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc gacttatgtt
     1561 ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt cgtgttgcga
     1621 ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc catgggaacg
     1681 agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt tatgggtgtc
     1741 gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc tgagcagaca
     1801 attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat cattgattct
     1861 gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt gaatctgcat
     1921 aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa cttggatgcg
     1981 atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg tgtgtcggca
     2041 ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac ttttctatat
     2101 ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt tcttaatccg
     2161 atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt gatcgaatat
     2221 cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg ggtaatgacg
     2281 gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa ggacaaatat
     2341 atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa ggtgttcaaa
     2401 agccagattc tcagcatt
//
LOCUS       DAXLPI010000197         2395 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXLPI010000197 DAXLPI010000000
VERSION     DAXLPI010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.206"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            316..570
                     /locus_tag="ACF3T5_002688"
     CDS             316..570
                     /locus_tag="ACF3T5_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFD3633193.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACF3T5_002689"
     CDS             571..888
                     /locus_tag="ACF3T5_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFD3633194.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACF3T5_002690"
     CDS             1447..2382
                     /locus_tag="ACF3T5_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633195.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAXLPI010000198         2275 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXLPI010000198 DAXLPI010000000
VERSION     DAXLPI010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.207"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            111..1724
                     /locus_tag="ACF3T5_002691"
     CDS             111..1724
                     /locus_tag="ACF3T5_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD3633196.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNNYTSARILAGLGIKAILIEE"
BASE COUNT          712 a          403 c          445 g          715 t
ORIGIN      
        1 atttctctat attttctgtc tattcaaaat aaacaataat tttccggcac aattatttgc
       61 tgttttttac cataacatag tacgatcaac gtagaaaagg agatgttttt atgaaagttg
      121 ttgtcattgg cggtgtagct ggcggtcctt catttgccac tcgtttccgt cgattgaatg
      181 aagcgcacga aatcattatc tatgaacgcg gagaaaatat ttcttatgca agttgtgctt
      241 tgccttatta tttaggaggt gtgatcactg accgtgactc gctgatcgaa cgtacaccag
      301 aaatattgaa aacaaaaaac aacatcgatg tctttactaa acacgaagta acagcaatcg
      361 atccttctac taagcgatta acagttaaag acctatccac aaatgaagaa ataaaaacag
      421 attacgataa gttgatcatc tcttctggtg ctagaccaga ttatccggat attcccggag
      481 tttttgaagc agaaaacggc tttgtcctcc gtagtgtgac ggatgcggat cgaatcaaat
      541 cgttccttga agaaaaaaat ccacaacatg tcgtcattct tggtgcaggt gttatgggtc
      601 tggaattagc tgagaatttc aagcatcgcg gcttagacgt gactttaatc gatcaattgc
      661 cacaagtcgc tttcccttat gatccagaaa ttgctaattt agtttatgac aaattgctga
      721 aagaaggatt agccgttcat ttagaaacaa gagttactga gatccgtgat aaaggtcgag
      781 aaatagtatt atcagatggc tccgtccttt ctgctgatat gctaattttt gctgttggtg
      841 tttctccgaa gaatgaagtg gtgaaagcaa ccggcataca attatcagat acaggtcaga
      901 tcattgttga tgaccagtta caaaccaatc tgccggacat ctatgcgatt ggcgatatta
      961 tcgaaacgac tagtgtagtg actggtcagc cgatccaaag tatgctttcc agtgcggcca
     1021 atcgtcaagg acatatgctg gcagatattt taaatggtac gcctatgcgc tatcgcggat
     1081 atattggtgc aggtgtcgca aaaatctttg atcatacagc aagttatgcg ggaatgacag
     1141 aacatgaatt aaaagcatca ggcataacaa attataaaac tgtttttatc actccctttg
     1201 accatgccta tttctatcca gaagctacaa gattaaatct aaagctgatt tttgatgcag
     1261 atagcggtcg tattttaggt ggacaagcat ttggagaaaa aggtgtcgat aaacggatgg
     1321 gagaactttc tgtagcaatc accggaaact tgacagtctt tgatttgcct gatttggagt
     1381 tgccttactc tccaccgtat tctactaccc gtgacccgtt gaatatagct ggttatgtcg
     1441 caatcaatca aatgacgaat atcgtagaaa cgatcaaagc aagtgatata cccgaaaacg
     1501 atttgaaaga agcgttcttt ttagatatac gtgaacctaa taaagcacct tctggcagta
     1561 tctcggcaac aaaaaatatt ccaatgaatg agttacgaga tcgaatcaat gaaatcccaa
     1621 aagataaaaa agtttacatt actttcagaa gaggattgaa taattatact tctgcccgaa
     1681 tcttggcagg tttgggtatc aaagcgattt tgattgaaga ataaaaaaac gaccgcggtg
     1741 ggtagccgcg gtctaggagt tatttttact tgaggagtaa aaatgaaaaa gtatataggg
     1801 ttgttggttg gtatgtatat agaatagaag ataaatgtga agaaattgtg cattatttct
     1861 tcacatttat taggttatag tttaagaaag tttaaacatt gcgcaattta attattttag
     1921 ctgtttcttt tcataaaaaa acatgttgtg actctgagac aaatcatagt ccatagtgat
     1981 gtctctagag taactacgcc taatattacg aggcatcata ttaatctatc agcactagta
     2041 cctaaactag catccctttc gaatattttt tcttatataa taaagtttgc tttcactcct
     2101 ctaatctcac caaatcgact ggcttgcgtt gtgtgatact aggaactgat tggtttagaa
     2161 cgacttttct attgtaatca tatttttcgc catcctcgtt gtcttctaat ccttcaatga
     2221 atgatcccta atgtgtgtat taaataattt aatttttttg ttcctattta tttaa
//
LOCUS       DAXLPI010000199         2273 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXLPI010000199 DAXLPI010000000
VERSION     DAXLPI010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.208"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..623)
                     /locus_tag="ACF3T5_002692"
     CDS             complement(<1..623)
                     /locus_tag="ACF3T5_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFD3633197.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKEL"
     gene            complement(634..1302)
                     /locus_tag="ACF3T5_002693"
     CDS             complement(634..1302)
                     /locus_tag="ACF3T5_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFD3633198.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1432..2016)
                     /locus_tag="ACF3T5_002694"
     CDS             complement(1432..2016)
                     /locus_tag="ACF3T5_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3633199.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          816 a          379 c          299 g          779 t
ORIGIN      
        1 attaattctt tcaaatagta aaaacgatgt tcagctataa caatagtaaa tcctttattt
       61 tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa atttgaagac
      121 ggttcatcca atataattaa tttagagttt agcgataaac tacatccaat agaaattttc
      181 tgccgttcac cacttgatag cgaaaaaatg tttttatcta atatatttga aagatctaat
      241 aattcagcta attcattgat tcttttatcc atatgctctt ttgtaaatcc aaagttttcc
      301 atcgaaaata ctaactcact cgttgtattt gttgtaaaga attgtcctct aggatcttga
      361 cttacgtagc caatattttt tgatatactt tcttgtgtta aggattgtag cgagttattt
      421 tcaaataaaa tttccccaac caattttccg tcataaagtc ttggaataag tccattcatt
      481 aactttaata gtgtggattt cccactccca gaagaaccag taatcaatgt aagactgcct
      541 ttttttattg aaaaattaag gtcaacaagc gcttgttcct taacatcctt atatgaaaaa
      601 gaaacattgt taaattcaaa caattctttc acctcaaatc aaaatactta agaaaaaaat
      661 agtaacggaa aacaggatga tatagtctaa taagctcatg tttgttttat agtaagaact
      721 tcttttcaca ggagattcta tcccctttgt tattgcatta gcggtcattt cttcagcaat
      781 catagttgca cgtataagaa cgggaactaa cagaaattcc aaatattgca gaggctgaaa
      841 aaaagacaag cgaatgtttc tcatttttaa agattcttta atcattctta tttcagaacg
      901 ataagtcgga aagaatctga tcactactgt aatacttaac attatttttt tcggtaaatg
      961 tattttttct aaagaggata ataattcatt gctagtatag acactaacca acaaccatga
     1021 gatcattacg accggcaaca ttcgaaatac aatatataag gatgtcgcaa aaaatatagt
     1081 aaaaaactct atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat
     1141 caagatgaaa taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat
     1201 aatgaaacac cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa
     1261 aatatttaaa gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa
     1321 taagatcgca ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga
     1381 tcttatcaaa aagcataact gagaacaatt ctcaatgcaa gcttttttta attaactcag
     1441 taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt
     1501 ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta
     1561 gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa
     1621 cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc
     1681 ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa
     1741 aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc
     1801 tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc
     1861 attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata
     1921 aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag tttgctgaaa
     1981 tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag ttataatata
     2041 atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat tatcttacac
     2101 aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag agctaaacta
     2161 atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa tagactcatc
     2221 taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta tat
//
LOCUS       DAXLPI010000200         2200 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXLPI010000200 DAXLPI010000000
VERSION     DAXLPI010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.209"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..503
                     /locus_tag="ACF3T5_002695"
     CDS             <1..503
                     /locus_tag="ACF3T5_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633200.1"
                     /translation="SNLIFEGKNLFLNLTISFEKDYHKLDFKEGAELTIKFTDLCSGK
                     CLVNNQSVIDFGDSKYISNFKNKIKAMKSREQSRGVFSKGGHGRKRKLDQDRWEAIKK
                     RESNFVKTYNNQCSAKIIQTAIRNKTQIICISEPDKNYGEWAYYQLKEQIINKGKRYG
                     IEVKSE"
     gene            complement(1063..1440)
                     /locus_tag="ACF3T5_002696"
     CDS             complement(1063..1440)
                     /locus_tag="ACF3T5_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFD3633201.1"
                     /translation="MKKSYPVIFSAEENGYSVVFPDFAGATQGDNFEEAMSNAKEFLD
                     GILAYYIDEDISVPSPTDIHQLNNLGNNKIVSLIQSDPSPFIQKKTVRKNVTVPEWLV
                     ARGEKDKINFSKVLTDALLKIYE"
     gene            complement(1486..1674)
                     /locus_tag="ACF3T5_002697"
     CDS             complement(1486..1674)
                     /locus_tag="ACF3T5_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFD3633202.1"
                     /translation="MPLTGKKMLKLAKKNGWKVVRITGSHHIVAKEGFPPVPIPVHSN
                     QDLKKGTEQKLLKDLGLK"
     gene            1866..>2200
                     /locus_tag="ACF3T5_002698"
     CDS             1866..>2200
                     /locus_tag="ACF3T5_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD3633203.1"
                     /translation="MNKKQDQEYYIEKLEKENLELKERIRYYETKFCKRGDCMKPNPV
                     ETGKRIKSIRSNFGMTMEQFADLIGNAQKGNINNWERGFSLPNKERLIKIAELSNKTI
                     DWIKWGTLE"
BASE COUNT          801 a          316 c          408 g          675 t
ORIGIN      
        1 agagtaatct aatctttgag gggaaaaatt tatttttgaa tttaacaatt tcttttgaaa
       61 aagattatca taagctagat ttcaaagaag gagctgaatt aaccataaag tttactgatt
      121 tgtgtagcgg taaatgttta gttaacaatc aatctgtgat tgattttggt gacagcaaat
      181 atataagtaa cttcaaaaat aaaattaaag caatgaaatc aagggagcaa agccgaggag
      241 ttttttctaa aggcggtcat ggtcgaaaaa gaaaattaga tcaagataga tgggaagcca
      301 taaagaaaag agaaagcaat tttgttaaaa cctataataa tcaatgttcg gcaaaaatta
      361 ttcaaacagc tatccgcaat aagacgcaaa taatctgtat ttctgaacca gataaaaatt
      421 atggggaatg ggcttattat cagttgaaag aacaaataat aaataaaggg aaaagatatg
      481 gaatagaagt taaaagtgag tagaataaga aatcgttgat ttggcaacga aatagtgagg
      541 ggacactcac tcactgaagg gttagctctg tcaagttgtg taaccagacg gaaggacgaa
      601 tattgttgag gttttagaac catgtaaaac aacacagttc tcaaatgtta cggataaacc
      661 gtttgcggga aatcaggttt taaaaccatg taaaacaaca cagttctcaa ataaggcttt
      721 attgaattga tcagtagttt ctgttttaga gttatataaa acgacatggc tcttaaatag
      781 gagctaacga agaaaaagaa ggtactccgt tttggagtta agtagaaata aaaaaataga
      841 atcgttgatt tggcaacgag ttgaaaaata cgcaaaacta tggtcaccaa gggataaccc
      901 tgaaactgat gatcgctata tcttactagt agttaaacag tctaaatagt actcatttat
      961 tgtattgatt ctaagggaac ctgcaataaa tgagtactat tttactttta attccgctat
     1021 ctatgtcatt aggcaactaa attgttatta atatatttac gtttactcgt atatttttag
     1081 taacgcatct gtcaaaactt tagaaaaatt tattttatct ttttcccctc tagctactaa
     1141 ccattctggg actgtgacgt ttttgcgtac ggtctttttt tggataaatg gagaaggatc
     1201 actttgaata agagaaacaa ttttgttatt gcccaaatta tttagttgat gaatatctgt
     1261 tggagaagga actgaaatat cttcatcaat atagtaagct agaatcccat caagaaattc
     1321 tttagcattg gacatagctt cttcaaaatt atctccttgt gttgctccag caaaatcagg
     1381 aaaaactact gagtagccat tttcttcagc agaaaaaatt acagggtaac tttttttcat
     1441 ttccaccact cctttcgagt taatccaatg aactaaagga ctgggctatt tcagccctaa
     1501 gtcctttagt aacttctgct ctgtcccttt ttttaaatcc tgattactat gaacaggaat
     1561 aggaacgggt gggaatcctt ctttggctac aatatgatgg ctgccagtta tcctcaccac
     1621 tttccaacca tttttcttgg ctagtttgag cattttttta ccggtaagtg gcattcgcga
     1681 ctacctcctt acaaatatta ttatacgcat tatgcgtata aaggtcaaga gtttttacat
     1741 taaaagtaga atgtaagtgt cacaataggg tataccctat tgtatcatat ggtaaagtac
     1801 agtataataa ggtttggagg tgtaacacta gggtgtaaca caatgtgaaa tttggaggtc
     1861 attttttgaa caagaaacag gatcaagaat attatataga aaaattggag aaagagaact
     1921 tagaactcaa agagcgaata agatactatg aaaccaaatt ctgtaaaaga ggtgattgta
     1981 tgaaaccaaa tcctgttgaa acaggtaaaa gaattaaatc aattcgttct aattttggaa
     2041 tgactatgga acaatttgca gatttaattg gaaatgctca aaaaggaaat attaataatt
     2101 gggaacgagg attctccctt cccaacaaag aaagattaat taaaattgct gaattaagta
     2161 acaaaactat cgactggatt aaatggggaa ctttagaaga
//
LOCUS       DAXLPI010000201         2139 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXLPI010000201 DAXLPI010000000
VERSION     DAXLPI010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.210"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..814
                     /locus_tag="ACF3T5_002699"
     CDS             <1..814
                     /locus_tag="ACF3T5_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFD3633204.1"
                     /translation="KPNIYGGLACRLRKVSYALNITGLGTAFQKKGVLKSLIVFLYRL
                     AAKKSKVIFFENFENMKTFQTLQIAKQSKYKLLNGAGVDVEHFSYLEYPKDEQVHFLF
                     IGRIMKEKGVDELFEAMKNLYSEEKNCVLDVLGYFEEDYGIKIEKYEKEGWLKYHGYQ
                     SDIRPFVERAHCFVLPSWHEGMANTNLESAASGRPIITSDIPGCKEAVIESKSGYLCE
                     RKNSCSLYNSMNKFLKLSYEQRINMGKMGRNYMIKKFNKKKVVDETIKSLFS"
     gene            821..1216
                     /locus_tag="ACF3T5_002700"
     CDS             821..1216
                     /locus_tag="ACF3T5_002700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3633205.1"
                     /translation="MFCKVCDRKEGFCGFRIDIKELAKVSDIFLFTTLQEGMPRSMME
                     AMASGLPCIASKIRGNVDLLVEGKGGYLVPAKDAELVAECLRELINNKSLRKYMGNEN
                     LSRIKEFEVSIVKKRIKEIYEEVIGESVK"
     gene            1213..>2139
                     /locus_tag="ACF3T5_002701"
     CDS             1213..>2139
                     /locus_tag="ACF3T5_002701"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3633206.1"
                     /translation="MKNPMFSIIVPIYCVEKYLKDCLDSIRNQTFKNFEAILVDDGSP
                     DECPEICDYYSQIDGRLKVIHKKNGGIIDARKIGVTSAMGEYIVFLDGDDWLANTYLE
                     KYAEVITKYNPDIICSGYFASRSNKIRKRELSCSFGYYDKNKIINLIYPKLIEDEHGE
                     YFSPQLWAKAFRTTLYKEQQQVSGQVSVGEDHACTKPCIYHANSIYMMEKCLYYYRIN
                     ENSITKKPQPYYWEGPKAIGEHFEKQIDMNKYDFQDQVYRNVTHNLFNVAVSQFNKQD
                     KYLNIKKDIIQNLSDEYYTRAICKCKYKDNWKG"
BASE COUNT          848 a          234 c          414 g          643 t
ORIGIN      
        1 caaaccaaat atatacggtg gattggcatg tcggttaaga aaggtttctt atgcattaaa
       61 tataaccgga ttaggaacgg catttcaaaa aaagggggtt ttgaaaagtc tcattgtatt
      121 tttgtataga ttagcagcta aaaaatctaa ggtcatattt ttcgaaaatt ttgaaaatat
      181 gaaaacattt cagactttac aaattgctaa acaaagtaaa tacaagctat taaacggtgc
      241 aggagtagat gtagaacact ttagttattt agaatatcct aaagatgagc aagttcattt
      301 tttatttatt ggaagaatta tgaaagagaa aggtgtagat gaactttttg aagcaatgaa
      361 aaacttatat agtgaagaga aaaattgtgt acttgatgtc cttgggtatt ttgaagaaga
      421 ttacggcata aaaattgaaa aatacgaaaa agagggatgg ttaaaatatc atggatatca
      481 atctgatatt cgtccctttg tagaaagagc acattgcttt gttttgccta gttggcacga
      541 aggaatggca aatactaacc ttgaatcagc agcatcaggg agaccaatta ttacaagtga
      601 tatcccaggg tgtaaagaag cagttattga atcgaagtct ggttatttat gtgaaagaaa
      661 aaatagttgt agtctctata attcgatgaa taaatttcta aaattatcat atgagcagcg
      721 cattaacatg ggaaaaatgg ggagaaatta tatgattaaa aaatttaata agaagaaagt
      781 tgtcgatgaa acaattaaat cattatttag ttaaaaagat atgttttgca aagtatgtga
      841 tagaaaagag ggattctgtg gcttcagaat agatatcaaa gaattggcaa aagtttctga
      901 tattttttta tttactacat tacaagaagg aatgccaaga tctatgatgg aagctatggc
      961 aagtggattg ccatgtatag caagtaaaat ccgaggtaat gtagaccttt tagtggaagg
     1021 aaaaggtgga tatcttgttc cagctaaaga tgcagaatta gtagcagagt gtttgagaga
     1081 actaatcaat aataaatctc ttagaaagta tatgggaaat gagaaccttt ccagaattaa
     1141 agaattcgag gttagtatcg taaaaaaaag aatcaaagaa atatatgaag aagttatagg
     1201 tgagagtgta aaatgaaaaa tccaatgttt tcaataatag tacctatcta ttgcgttgaa
     1261 aaatatttga aagattgctt agattcaata agaaatcaga catttaaaaa ttttgaagca
     1321 atactagttg atgacggtag tcctgacgaa tgcccagaaa tatgtgacta ttattcccaa
     1381 atagatggac gtttaaaagt aattcataag aaaaatggtg gaataataga tgcacggaaa
     1441 ataggagtca ctagtgctat gggagagtat atagtttttc tagatggtga tgattggctt
     1501 gcaaatactt atttagaaaa atatgcggaa gtaattacta agtacaatcc agatataata
     1561 tgtagtgggt attttgcatc acgttccaac aaaattagaa aaagagaatt gtcttgctcg
     1621 tttggatatt atgacaaaaa taaaattata aatttgattt atcctaaatt aattgaggat
     1681 gaacatggag aatattttag tccacaatta tgggcaaaag cttttagaac tactctgtac
     1741 aaggagcaac aacaagtatc tgggcaagtt agtgttggtg aagatcatgc atgtacaaaa
     1801 ccatgtattt accatgcaaa ttcgatatat atgatggaaa aatgtttata ttattacaga
     1861 ataaacgaaa attctataac aaaaaaacca caaccatatt attgggaagg accaaaggct
     1921 attggagagc attttgaaaa gcagattgat atgaataagt atgattttca agatcaagta
     1981 tatagaaatg ttacacacaa tttatttaat gttgctgttt cacaatttaa taaacaggac
     2041 aaatatttaa atataaaaaa agatattata caaaatctaa gtgacgaata ctatactaga
     2101 gctatttgta aatgcaaata taaagataat tggaaagga
//
LOCUS       DAXLPI010000202         2127 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXLPI010000202 DAXLPI010000000
VERSION     DAXLPI010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.211"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>2127
                     /gene="acm"
                     /locus_tag="ACF3T5_002702"
     CDS             <1..>2127
                     /gene="acm"
                     /locus_tag="ACF3T5_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFD3633207.1"
                     /translation="CRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVDPTN
                     ITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLT
                     VRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVT
                     KPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPS
                     TFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMV
                     SYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKI
                     FKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKE
                     APNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYF
                     KLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYV
                     PSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKE
                     VSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIP
                     GSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSK
                     SILPKTSSKKSSFAVIIGLLIVTICA"
BASE COUNT          830 a          346 c          413 g          538 t
ORIGIN      
        1 agtgtagaaa tattatttta tcaatgttat ttattattac taatataaca tcactgattc
       61 ctgttcatgt ctatgcggat gcaggcagag atatcagcag taatgtcact tcgttgaccg
      121 tggacccaac gaatattaca gatggaggaa acataaaagt aaaattctcg tttgacgaaa
      181 aaaaacaaaa tatccaacca ggtgattatc tttggatcaa ttggccaagt gaaggaaata
      241 ttcgcggtga aggattccaa aaagaaatcc cattaatgat tgaaaataaa aatgttggaa
      301 ctttaacggt cagaaaagat tctgcgcagg ttgtgtttaa tgaaaatatt aaaaacctag
      361 actcagtaga aggttggggg caatttgaga tacaggccag aaacgtaacc gataccggtg
      421 aagaaaatac gggaccattc accgtgacga gcggtgataa aacagctact gtaaatgtga
      481 caaagccagc ttctggttct tcttctagcg ttttttatta taaaacagga gacatgctac
      541 ctgaagatac taagcacatt cgatggtttt tgaatattaa caataacggc acttatgttg
      601 aacaaccagt taaaatcagt gatgaaatcc aaagtggaca aagattagat ccaagtacgt
      661 ttgaaattaa tcaaatacac ttaggtgaac agaaggttta tagaggagaa gagggaatcc
      721 aacaattttt acaagatttc cccagcgcta catttaattt ttcagtaact gataattaca
      781 ttgaaataac tataccaaaa aactttgtca atcttaggaa aataatggtg agttataaga
      841 cgatcataga aaacccagaa caaataaatt ttgagaatca ttcggaagct tggtttaaag
      901 aatttaataa acctgctgtt gatggagaaa gtttcaatca tacagttaaa aatatttcag
      961 cttctggtgg agtcaacggt acagttagag gcgaattgaa gattttcaaa tatatcaatg
     1021 acacagagat tggtatccca aatgtgactt tcgaattaag acgagcagat gaacagccta
     1081 ttcaaggaca gtctagtatc ctgttgactt cgaatgaaca gggagaagct agtatcaaag
     1141 gcctgcaagt aggagactat gttgtaaaag aaaaagaagc acccaattgg attgattttg
     1201 atccactaag ttcaaatgaa ctcaaattct ccataaacga aaatgataca gagggagtca
     1261 gtttacctat ttataacaag aaaaaggtta cgaacatcac agcaactaaa atttggaacg
     1321 gtggaaccac tccaagaccg agtatttatt ttaaattatt cagagcaagt acaaacaatt
     1381 gggaaccagt tcctgatgca gaaacaaaac gattagacaa tggaataact tcagtgactt
     1441 gggaagatat ccaacagtat gatgatagtg gaaacgaata tacgtttaaa gtgcaagaag
     1501 tagatcaaaa cggaaatgat tatgtccctt caggttatca aaaaattgaa aacgggctag
     1561 tcgttacaaa tgagaataaa gaagtcattt cagtctcagg acaaaaaaca tgggaagaca
     1621 atgagaacca agacggcaaa cgaccgacga cgatcacagt aaatctatta gccgacggtc
     1681 aaccgatcca acacaaagaa gtaagtgaaa aagatgattg gcgctatcgt ttcacaaatc
     1741 tgccaaaata cagagacggg caagaaatca tctatactgt aacggaagac aacgtgcccg
     1801 aatacagtac gacgattgac gggtataaca tcaaaaacag ttatacaccg gaaaaaacaa
     1861 atatatctat accaggtagc catataacac cgtggaaaaa ttttccagag aagacagaga
     1921 gagagaaaat tagaaatcta ttctctcaaa aattgcaaat atttgctcat acaggagaat
     1981 ccagcgaaca aagaagcaat tatattagta agagagctgt acctaaacag ataacaaata
     2041 aatcaaaaag cattcttcct aaaacaagta gtaaaaaatc tagttttgct gtaatcattg
     2101 gtttattgat agtaactatc tgtgctg
//
LOCUS       DAXLPI010000203         2105 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXLPI010000203 DAXLPI010000000
VERSION     DAXLPI010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.212"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            34..657
                     /locus_tag="ACF3T5_002703"
     CDS             34..657
                     /locus_tag="ACF3T5_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633208.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..>2105
                     /gene="trpX"
                     /locus_tag="ACF3T5_002704"
     CDS             1107..>2105
                     /gene="trpX"
                     /locus_tag="ACF3T5_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFD3633209.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAK"
BASE COUNT          749 a          346 c          446 g          564 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaa
//
LOCUS       DAXLPI010000204         2090 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXLPI010000204 DAXLPI010000000
VERSION     DAXLPI010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.213"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>2090
                     /locus_tag="ACF3T5_002705"
     CDS             <1..>2090
                     /locus_tag="ACF3T5_002705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633210.1"
                     /translation="DSAINKDLELASKATISESENSFGETTNNQENTNLSEDSIEVQP
                     ESIIQKEVLTDNDAIQQLEVHPHEDSDDAALVENQMTRVTEESIKSNTSQVDKDTTVL
                     SNTTRDLVNLEDTAIENVTPQKTIKKDSISLLEENNSSSASRTLRRSVRSLDVQTGEN
                     FLEDWALPDNSNISGSLITQTGITYVGVKTDDPTQLHLKMAYRAAATPASIDWIYLRV
                     DPELTKYIEEINIKNTVNIGAQVGYQRYFEKVDTNTLSNVNTFGTPTGVSDAYAQVDQ
                     KTGGIYRVLLDKFFIRIPGTNPKSRGALSGNWGVFPSVPLEAERYEANIYIKLSKDVA
                     ELQQELGRDKFGVQLRLQGQSSSGVATIAKESLNKNTIVDLSQEEPDENNSNWLNSPS
                     VHTITTTYEGKQMGSDGQFSSILEGTDKSLIRVQNTIMNNLLYGTALDLGRPVSFNIE
                     IDPQILNLVSDDDYITIWQVYRENSINHHDGGSNAPVMLKKNMFVDNKIKIEVNKDKA
                     GQYGNGVYYVNAVESFMNTLSSSPFVGANFIDIPVMPKSLIEDEDQLISAEIKTWFTD
                     KKNDQERIVRNSLASSYINLFPTTINFYDYSTKDTEQLLKLRESTYLVSTFGQEINGM
                     DMGETYEISNYEFVTADPESLIITQKDGNIINLYYKKLSSDAEFYEPEVEKEEVNYGE
                     TIDLTDNVTNLEEL"
BASE COUNT          772 a          298 c          400 g          620 t
ORIGIN      
        1 aagatagtgc tataaataaa gatctagaac tagcttcgaa agcaaccatt tctgaatcag
       61 aaaattcatt tggtgaaact actaacaatc aagaaaatac aaatttatct gaagatagta
      121 ttgaagttca gccagagagt attattcaaa aggaagtctt gactgataat gatgctattc
      181 aacaattaga agtacatcct catgaagata gcgatgatgc tgctttagtt gagaatcaga
      241 tgacaagagt tacagaagaa agtataaaaa gcaatacttc acaagtagat aaagatacga
      301 cagttttatc taatacaaca agggacttag ttaatttaga agatactgct atagaaaatg
      361 ttactccaca aaaaacgatc aaaaaagata gtatttcttt attagaggaa aataattcat
      421 catcagcatc aagaacttta cgtagaagtg ttaggagtct tgatgttcaa actggagaaa
      481 actttttaga agattgggca ctaccagaca attctaacat ttctggaagt ttgattactc
      541 agactggaat tacttatgtg ggtgtaaaaa ctgatgatcc cactcaatta catttgaaaa
      601 tggcttacag agctgcagca acgcctgctt caattgattg gatttatctt cgtgtggatc
      661 cagagcttac gaaatatatt gaagaaatta acataaaaaa taccgtaaac atcggtgcac
      721 aagttgggta ccaacgatat tttgaaaaag tagatactaa tacattgagc aatgtaaata
      781 cttttggaac tccgacaggt gtaagtgatg cttatgcaca agtagatcaa aaaactggcg
      841 gaatctatcg tgtgttatta gataaatttt tcatacgtat tccgggcacg aatcctaaga
      901 gtcgaggcgc gctatctgga aactgggggg tatttccatc tgttcctctc gaggctgaaa
      961 gatatgaagc gaatatctat ataaaattat cgaaagatgt cgcagagtta cagcaagagc
     1021 ttggaaggga taagtttggt gttcaacttc gtttacaagg tcaatcaagt tctggtgttg
     1081 caactattgc taaagaaagt ttaaataaga atactattgt tgatctttca caagaggagc
     1141 cagatgaaaa taattcaaat tggttaaact caccatctgt ccatacaatt actaccactt
     1201 atgaaggaaa gcaaatggga agcgatggtc agttcagtag tattctcgaa ggaactgata
     1261 aaagtctgat tagagttcag aatacgatca tgaataacct tttatatgga acggcacttg
     1321 atttaggtcg acctgtttca tttaatattg agattgatcc acaaatatta aatttagttt
     1381 cagatgatga ttatattacg atttggcaag tatacagaga aaatagtatc aaccatcatg
     1441 acggtggatc aaatgcacct gtcatgttga aaaagaatat gtttgtagat aataaaataa
     1501 aaattgaagt caacaaggat aaagctggtc agtatggaaa tggagtttac tacgttaatg
     1561 ctgttgagag ctttatgaat actttgagtt cttcaccatt tgttggtgca aactttattg
     1621 atattcctgt tatgcctaaa tctctaattg aagatgaaga tcaattgata tcagcagaaa
     1681 ttaaaacatg gtttacagat aaaaaaaatg atcaagagag aatagtaaga aactcacttg
     1741 cttcaagtta cattaattta ttccctacta ccattaattt ctatgattat tcaaccaagg
     1801 atacagaaca attattgaag ttacgtgaat caacctactt agtttctacg tttggtcaag
     1861 aaattaatgg aatggatatg ggggaaacat acgaaattag taattatgag tttgtgactg
     1921 ccgatcctga aagtttaata atcacacaaa aagatggaaa tatcattaat ttgtattata
     1981 agaaactttc tagtgatgct gaattttatg aaccagaagt agaaaaagaa gaagtcaact
     2041 atggagaaac gattgatttg acggataatg taactaatct ggaagagttg
//
LOCUS       DAXLPI010000205         2068 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXLPI010000205 DAXLPI010000000
VERSION     DAXLPI010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.214"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..457
                     /locus_tag="ACF3T5_002706"
     CDS             <1..457
                     /locus_tag="ACF3T5_002706"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFD3633211.1"
                     /translation="ILLVVLVPRIFGLTPYIIEDKEMSPYYAQGNLIYVRPKNTNEIL
                     VGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQI
                     PYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            585..1268
                     /locus_tag="ACF3T5_002707"
     CDS             585..1268
                     /locus_tag="ACF3T5_002707"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFD3633212.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1577..1650
                     /locus_tag="ACF3T5_002708"
     tRNA            1577..1650
                     /locus_tag="ACF3T5_002708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1611..1613,aa:Arg,seq:tct)
     gene            1809..>2068
                     /locus_tag="ACF3T5_002709"
     CDS             1809..>2068
                     /locus_tag="ACF3T5_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFD3633213.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLIS"
BASE COUNT          694 a          327 c          425 g          622 t
ORIGIN      
        1 tattcttctc gtcgtcttgg ttccccgaat ttttggtttg actccttata ttattgagga
       61 caaagaaatg agtccatatt atgcacaagg aaatctgata tatgtacgac cgaaaaatac
      121 aaatgagatt ttagtcggag atattattac atattatgaa aattcaggaa aaaaaatctc
      181 gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa aagctgatac
      241 aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta gtcccatctt
      301 tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga ttcaggggtt
      361 attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct ttgtagagtt
      421 gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat
      481 tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat gcagcttgta
      541 gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc aatgaaatga
      601 tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag caaaaaaaat
      661 taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta ccagttattc
      721 cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct gaacgaatct
      781 tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca atcggagaaa
      841 acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact
      901 atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca gcagatatct
      961 tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa tcaaaatata
     1021 ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg atggtagatg
     1081 acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt ggcaaacgag
     1141 ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc gatttgacat
     1201 cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca gaaacaatcg
     1261 agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg gctcaattcg
     1321 aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga aatctaaata
     1381 aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct tagaacagct
     1441 gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat aattcattat
     1501 ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta taattattga
     1561 tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct tctaagcgga
     1621 cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta
     1681 acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa
     1741 aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc agaaaagagg
     1801 aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt gaattttttt
     1861 atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg gtttttaaaa
     1921 tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac caaggagtgt
     1981 acaatggact attaggagtg ggactacttt atggagctta tctttcttcc gcatctaaag
     2041 aaatcacaag tatgctatta atatctat
//
LOCUS       DAXLPI010000206         2059 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXLPI010000206 DAXLPI010000000
VERSION     DAXLPI010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.215"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            79..831
                     /locus_tag="ACF3T5_002710"
     CDS             79..831
                     /locus_tag="ACF3T5_002710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3633214.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(853..2007)
                     /locus_tag="ACF3T5_002711"
     CDS             complement(853..2007)
                     /locus_tag="ACF3T5_002711"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFD3633215.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT          672 a          383 c          388 g          616 t
ORIGIN      
        1 agagatagaa tagataaaag aggagaataa tttattccga aaagtgaaaa catataacaa
       61 aaaattagag gaaatcaaat gaatgaaaaa gaatgggatg aatttgctga agaatattat
      121 gaaatccagc aagaatcgca agtaactatt gctgaagatg taaaaaatta tctgaaaacg
      181 gagaaagtcc tgcctgctga aacagcagca gacgttgctg gaggatctgg cagatttctt
      241 tccttagcaa aagaaattaa tcgctatgat ttgatcgatt tttcttcagg aatgctagaa
      301 aatgcaaaaa aagaagcaga aaaaaatgag ttgccaaatg tttcctttat caaacagtcg
      361 ttagaagaat ttatccaatc aaatgagaca tatgaaatgg tttttacagc agcaaatcca
      421 gcattggtac atcttattca attagaaaaa ttgatgaaaa agtcaagcaa atggtgcgtg
      481 ataatcaggg tagtggaatc aatcgatgat gttttttatc ctatcgaaaa gcaactagga
      541 atgatagaag aagatcctaa tacatcgcca gttgtaatgg atcaatttga aacatactta
      601 attgatgaag gattcaaagt gaagaaaaaa gattttactt attttacaca ggaatcaatt
      661 actgaagaat tgatgaagct atactatgac gcgtacgcaa aagatcctgt tgtgaccagg
      721 tacattcgca aactattcag tgaaaaacga gaaatcctta gtacgactaa gctgacctat
      781 agactgctcc tttggtccgc agataaaaaa agaacctttc acgactcgtg aagggttctt
      841 tttatgattt tattatattt tgtagtaacg aatatcattt tcgatctttg taatcgaaaa
      901 aggtacatct gaagtatatt ctgcacattc tccgtgttca ggaagcgtct cactgaaaat
      961 cgtttcatat tcgtcaacag tgatccgtgt tcgagaatct agcatctctt gatgagattg
     1021 tgcgaagaga tattcttgat agttttcttc cagataacct gtaaaaaatt cacttactgc
     1081 accagaaccg tagctgaata gaccgatgcg atctcctggc tgtagtgatt tcgaattttc
     1141 cagtagagaa gttagcccca ggtataatga accagtgtaa agattaccga ttcgtcggct
     1201 gtaacggatg ctttcttcat agcgagccat aagacgttcc tgattttctt cgtctgtttg
     1261 gtctaatacg ctttgcaatg ccttttttcc catcttagta tacggaatat ggaaagcaat
     1321 cgcctgataa tcttcgagtc ctcgacccga caattcttta tgtcgattcc aaactttttg
     1381 gaatgattcg atatacgtag aattagataa aggaccatca acaacaggaa attcgctata
     1441 atctggacgc cagaaatcat agatatcttc tgtcagaaat acgctgtcgt cttcaatcga
     1501 taaaatacgc gggttttgag tgatcatcat cgcaacagca ccgacacctt gcgtcacttc
     1561 accaccgctt gccaagccgt aacgagcaat atcacttgct atgactagta cttttcgttc
     1621 tggatgattt ttgacatatt ctttcgccat gtgcaaagct gcggtgccac cgtagcaagc
     1681 ctcttttact tcaaaagaac gagcaaaaga ttggattttc agtaaatggt ggataattac
     1741 cgcactcgct tttgaatgat caatcccgga ttctgttccg acgatgacca tgtcgattag
     1801 ctcacggtct ttttctgtta cgattttgct ggcagcattt gctcctagtg taatgatgtc
     1861 ttcatttgca cgattgactg ccatttggtc ttgaccaatc cctatatggt attttgcagg
     1921 atcatcccca cggctttctg ccaactctgt catatctaaa tataaattag gaataaaaaa
     1981 ggaaagacga tcaatcccta ttttcatttc aacacctctt tacttatgat aaaaaactta
     2041 ctttactaag gtatcataa
//
LOCUS       DAXLPI010000207         2059 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXLPI010000207 DAXLPI010000000
VERSION     DAXLPI010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.216"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(85..495)
                     /locus_tag="ACF3T5_002712"
     CDS             complement(85..495)
                     /locus_tag="ACF3T5_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFD3633216.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(676..1110)
                     /locus_tag="ACF3T5_002713"
     CDS             complement(676..1110)
                     /locus_tag="ACF3T5_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFD3633217.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTDNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(1210..2058)
                     /locus_tag="ACF3T5_002714"
     CDS             complement(1210..2058)
                     /locus_tag="ACF3T5_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFD3633218.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
BASE COUNT          694 a          412 c          324 g          629 t
ORIGIN      
        1 cttgattaac taaatataga tcttccattt ttccacctcc gctgttttac tatagtgtat
       61 catagcggat ttacacaaaa aagtctattc actaactgct ttcagagtaa cttctatatt
      121 tccagctgta gcttttgaat acggacacac tttatgcgct gcctctaata attcttgcgt
      181 ttcttccaga gaaagacctt cgatagatcc ttcaatctct acacctaaga caaagctcgt
      241 ttcactttct gaataaaggg aaacttgtgc actgacagta gaagccccag agatattttt
      301 ttgctgtttg atcaattcta atgcactgtt aaaacatgca ctgaaaccag ctgcaaacaa
      361 ttgttcaggg ttggttgcat tttcgacgcg tttaccagga gctgcaatat tcaattcgaa
      421 tgtacgatcg ggattatgaa cttctccaga gcgtccgcct gtgttgatca ttttcgtaga
      481 atacattttt ttcataaaat taccaccttt aattatattt tatacaattg aattttacac
      541 aatataattg tggaaagcaa agaaatggat tggcataaga taaataagag aaagcacatg
      601 aaacagacgc gctttctcga atagcaaaca tatacaaaaa aatatcagag acaaaaacta
      661 atctcatcat aaacattaca tatatttctc aaaaagttgt tttctttctt caaaacttac
      721 ttcacgatgg acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt
      781 agtcacacca aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa
      841 tgctttatta tctgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg
      901 atgttcagca gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct
      961 cgtgccatac aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat
     1021 cagttgtatt tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa
     1081 tacaaaatct atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag
     1141 ttactagatt cttttattat accagttaaa gaaaacgcat tcaaaaaaat agcggattta
     1201 cttaccaact cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct
     1261 gctgacaatg acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa
     1321 ttcatgatca gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt
     1381 tgcctctagc agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc
     1441 caaataatgt tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata
     1501 agtcatgaca attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc
     1561 aaaaacaaac agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt
     1621 attcaagtgg aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga
     1681 gttgttttta tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga
     1741 caacaaccga tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaaa
     1801 caagcttccg aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac
     1861 caacataacg atattcaaca ctaaaataac tatagctcgg tcaaatccta aaacagcttc
     1921 cagtaatatt cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat
     1981 attgatagcg actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa
     2041 ttgtctcgtt tctttcatt
//
LOCUS       DAXLPI010000208         2027 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXLPI010000208 DAXLPI010000000
VERSION     DAXLPI010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.217"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..582
                     /gene="mobL"
                     /locus_tag="ACF3T5_002715"
     CDS             <1..582
                     /gene="mobL"
                     /locus_tag="ACF3T5_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HFD3633219.1"
                     /translation="MNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVL
                     TKEPEYQEYIQISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQD
                     SQIKPLIESVDQMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLR
                     QLNRTINAHEEKYHASKAYEEQQRRIQMEQERQ"
     gene            603..923
                     /locus_tag="ACF3T5_002716"
                     /pseudo
     CDS             603..923
                     /locus_tag="ACF3T5_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1084..>2027)
                     /locus_tag="ACF3T5_002717"
     CDS             complement(1084..>2027)
                     /locus_tag="ACF3T5_002717"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFD3633220.1"
                     /translation="GPHAGGKHYPKKGFNHIRSKVKMSDVFEFIQSNVHDHDVKQMCT
                     VLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHKILVGRGETL
                     SLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSINQKWVTDITY
                     IHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTPSENLIIHSD
                     LGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYPSKAYENFEA
                     AYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          667 a          352 c          423 g          585 t
ORIGIN      
        1 atgaacttat taaatgaagt atataaaacc ctacctgaca aacgttcgga atggaattac
       61 ggggatgaaa aacgaaccaa actttcacca tacacaagag agaaactcga tgagttaacc
      121 aaatatgttt taacaaaaga acctgaatat caagaatata tacagatttc aaaagcattg
      181 aaagaagaag caaaaagtct ttatggggaa agcaagagag aatcaaagga tgcagagaaa
      241 aatgctctct ttgatttaaa aaagcgcaca ggaaatgcca tcctaacaga gctgaagaga
      301 caggatagtc aaataaaacc attaatagaa agtgttgatc agatgcaatc tatttacaat
      361 gaacgatcct ttcgaacgct taaacaattg ccaacgaatg aatacaaata tagttacgaa
      421 gaattcatga agcgcaggaa acaattccaa caaagagttg tcacgcagcg gagtttgcgt
      481 caattaaatc gaacgattaa tgcccatgaa gaaaaatacc acgcaagtaa agcatacgaa
      541 gagcaacaac gaagaattca aatggaacaa gaaagacagt aaagacagta ggaggaatag
      601 caatgaaaca atatttagat caatggaaag ttatagaagg atcattaaga gaagaacgaa
      661 tcgaacaact tcctgattgt ttagaaaaag agcatttgtt ccagataaga gaaatgttga
      721 gaaacgaaca gtttgatcca aatcaatttt tagtcgttga gtacccagct actggtgtgt
      781 attgttgtaa tcatgtgaat ggtgaaaaat attttatcat tcaagaatat gagggcaagt
      841 tagcacctta ttatactact tgggaaatga atgaagaggg aatcaataac tttccatgta
      901 aaagcattgg atttgagtca atacctcgga cacgatttgt gagaattttt tcgagattcc
      961 agcgggtgag tatctcatcg tttcgaccag cggtcaaacc aaaaccgttt gaccgctgat
     1021 cgaagcgatg aagttgaaac cctgtggaaa tctcgccaaa aaaatgtaag tggtagctag
     1081 gaactaagct tgcaatgcca actcctccat ctgatttggt gttaaatagt tgatactact
     1141 atggattcgc ttgcgattgt aaaatccttc aatgtattga aataacgcta aataagcagc
     1201 ttcaaagttt tcatacgctt ttgatggata gacttcttct tttttgagtg aggcgtgaaa
     1261 cgattcgata ccggcattat cgtagggaca gcccttacga ctgaaggagt gccgaatgtt
     1321 caattcattc agctttgcct cataggcttc gctagtgtac tgagaaccca aatctgagtg
     1381 gatgatcaag ttctcggagg gtgtttggtt taagagcgca ttgtcaaggg tttcgataac
     1441 caattctttc gtcatctgct tgccaaactt ccaaccgatg actttctttg tgtggaggtc
     1501 ttgaatagtg gagaggtagg tccagccatc ttttagggta tgaatatagg taatatctgt
     1561 cacccatttt tgattgatcg acgtagtggt aaaatcttgg tttaacaggt ttttacgtcc
     1621 ttcactttta actttcgctt gtttgccagg cttgtacttc ttgagggtga ctgaacgcaa
     1681 gcctaatttt ctcatcagta tttgtacttt ctttaaggac aaggtctcgc cacggccaac
     1741 aagaatctta tgaattttag gcgcaccata gatacccttg ttttcaaagt agattcctga
     1801 aatcaagcgg tttaagcgtt tgttttctga ttggcgcttg gaaggtttct ttttcttcca
     1861 gtcatagtag ctggatttcg gtgcttccaa aacggtacac atttgtttta cgtcatgatc
     1921 atgaacattt gattgaataa attcgaagac atcactcatt tttacttttg agcgaatatg
     1981 gttaaagcct tttttaggat agtgttttcc tcctgcatgc gggccat
//
LOCUS       DAXLPI010000209         2009 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXLPI010000209 DAXLPI010000000
VERSION     DAXLPI010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.218"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(22..1722)
                     /locus_tag="ACF3T5_002718"
     CDS             complement(22..1722)
                     /locus_tag="ACF3T5_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633221.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNIRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFNKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKHLRA
                     "
BASE COUNT          702 a          342 c          384 g          581 t
ORIGIN      
        1 ggatgcattt atcccttttg cctaagcacg taaatgcttg tatctatgat aaataaataa
       61 acatagcaag gtaaaccagc caatcaaagt aagagaaaga atgaaataga aatagattgg
      121 cgtttgataa tgaagcgtga gctcgttgat tcctttttgc tgcttcacga ttggggtccc
      181 gatatctgtt acttcataat cagtcaatgt ttttccattt tgctgtagca cagtccgatc
      241 gtagacgata acagggatat gaacagtttt gtccccagtg tttttccact tgattgtcag
      301 aagaccgcct ggatgtgttt ttataaaagt tgtttcgttt tctagtattt cttcgttata
      361 ccggtcgtat ttattgtctt ttgtctcctt atagattggc agatagtcag gtgtggtttt
      421 ttggaaaacg aacaagctgg tagacatatc tttgttgaag aatgttttcc ttgcttcttc
      481 cggcgtatcg aatagataag tatgtcgact gacaaatgtt tcattttccc acttttccag
      541 atgctggtat aagttctgga tcgtctgtgc tgaaaaaata agaatgccaa atatacttaa
      601 aagacggaac cacttttgtt cagaccactg gttcagtgaa agggcaaggt aaagtagaag
      661 caatagcgta aatggcacga aaaatcgaaa tggaaactgg atcaaactaa taaaaggcac
      721 atttttttcg atcaatgtcg tccatggaat caagctagta gaaagaaaaa agaaaacaag
      781 acttgtgtac aacacgattt tttggaataa ggtgttcttt tgttgaaaga aaataaaaag
      841 caaaaaacct gataatatca atataggaaa ggccttaggg taatggagcc aataactgcc
      901 atccagatct atcgcctttt gactcatttc tctgttaaca aagggaggaa gaatcttatt
      961 cccactgtaa acaatagcta atcctgccca aatatttgct gtcagaacaa aataaagcaa
     1021 gaccgactta atcagttgaa gcagtgcttt tcctttttca gtctggttaa aaaagtaata
     1081 aagataaaaa ggaatataca tcaaagctaa catgatcgaa gacagtaaat ggatctgtgt
     1141 cataagtccc actgagacag cgactttgat aacaggaaac tttttatgaa ggacgaaatc
     1201 agcaataggg atcatacata aaggataaaa acaagtcccc cagctagtga atccttggtt
     1261 gatagaccag tattgaatag aatacgtact catgaataaa acggctactg gtacacttac
     1321 ccaatttcga atattgacat atctcagtaa aatcagcata gaaaatcctg aaataagata
     1381 aagaatgaaa tttgaaagaa tctgataatg aaaccagcta cctgcaagca acacgatcaa
     1441 tccttgtaaa taagcgaaaa atgggccgta aacggcattc acgatcctgc cactttgctg
     1501 gaatccataa agggaaggaa aataatggaa atccccatat ttaatttgtt gcgcggcatc
     1561 atagaaccga ttgtaatgga atggggcatc agcacccaaa attagtttat gtgtgaataa
     1621 atgaggcatg agaaatgcca aagcaatgaa aaaaattgct aaaaatgggc catatttttt
     1681 caattgtcga actcctttgc aaactttaga taaattcatc atatatccta tgcgagaggt
     1741 aggcaagaaa ttagaaaatt catgaaaata tttatgaatt ttacaagaaa aacataagtg
     1801 aaagttctaa ggagagagga tgcgacataa gttttgttgc atctccttgt tttgaaacag
     1861 aatttttctt aatactcgaa acaaagtagc tttttcatga cctctcggta aacggtgttc
     1921 aatcagctgc ttctcggtaa taagctcact agcctagaaa atatgaaaag ctattttctg
     1981 actagcgctc ttattactca aaacataac
//
LOCUS       DAXLPI010000210         2006 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXLPI010000210 DAXLPI010000000
VERSION     DAXLPI010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.219"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            60..1361
                     /locus_tag="ACF3T5_002719"
     CDS             60..1361
                     /locus_tag="ACF3T5_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFD3633222.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(1430..>2006)
                     /locus_tag="ACF3T5_002720"
     CDS             complement(1430..>2006)
                     /locus_tag="ACF3T5_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFD3633223.1"
                     /translation="QEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVSKATIFKYF
                     GSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRVNKQFYIDL
                     MTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLILYFQAMVE
                     GISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT          661 a          388 c          351 g          606 t
ORIGIN      
        1 tcttcgttac aataaggaat gggacaagta aaaaaatgaa aaagcaaggg acgaggtata
       61 tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc tttaactatc gttattttaa
      121 ttctaagttt gttttttgtc atccgatcat ctacaaaaaa agaaccaatc attcctgaaa
      181 aaaaaataac agaaaaagca aaagaaccga cagatgataa gcaacaacta gttactcaag
      241 caaaaaatct ggctcaaagc tacgattatg accaagcgat tgcgttattg gaaaaagata
      301 gtaagaacgg atctactgaa acacaacagc ttcttcaaac actaaaaaag gagaaagaac
      361 aactgatcga ctggtctgat cctactcaga tctctcatgt ctttttccac agcttgatcg
      421 tggacccgca aaaagccttc cactctcaac aggcacaagg atacaaagat tatatggtga
      481 ctgttgaaga attcaaccgt tcgatcgaac aactttatca aaatgatttt gttttagtta
      541 atttaaatga tttgatccaa aaagacgaac aaggaaatct ttcctttaac gggcttgcgc
      601 tacctgaagg aaaaaaacca ttggttcttt cacaagatga tgtcagctat tatgaatata
      661 tggataattc cggttttcct agccgattgg tcgtatcaaa taacaatgaa atcaaaaatg
      721 tctataaaga gaaagataaa gaagaaatcg gtgactttga catggttcct ctgatcgatg
      781 cctttgtcaa gaaacatcct gatttttctt atcgaggcgc aaaaggtacc ttggccctga
      841 caggttacaa cggagtttta ggttatcgaa catcaaaatc tcaatatgga gacaatgaaa
      901 aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga acagttgaag aaagacggct
      961 ggagctttgc ttcccataca tggggtcact tgaatatgac tcaagcttca ctagaagatg
     1021 taaaaaaaga caatgaattg tggcagcaag aagttgcacc tatcctagga aaaacaaata
     1081 ttctgattta tccatttggt gcagacatca gcgactggca gccttattca gcagaaaatc
     1141 aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt ctgtaatgtc gatgcttcta
     1201 caccagcttg gggacaatta ggtacggatt tctatcgaaa tgctcggatc aatatcgatg
     1261 gcattcgttt tcaatctgat ttagacggaa aaaatcctat tcttgatcca ttcatcaatg
     1321 tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg attttacgtc atttatgaac
     1381 caaaaaagcc gaactgacat tttatcgtca gtttcggctt ttttatgctt tatttcttct
     1441 tcggtgcgat tcccttgatc aggacttctg tccattctgc tgtatatgga agcagttttg
     1501 cgtatatctc cggcttagaa attccttcta ccattgcttg aaaatagagg atcaagaact
     1561 catccgaata tttcaaatcc acttttcctt ctttccgccc tcgatggaaa agatcaagca
     1621 tgatactgaa actttcttgc gtatacgcat ccatcgcgtc agaaaattct tttttttctt
     1681 tttttgtata gtatgtcatc aaatcgatat aaaattgttt gttcactcga ttgagataac
     1741 ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa tggaagattt tccgcaatca
     1801 acgctttcgc ttcttgccct aaatcattca taaatgcaaa aaatacttca taaatcagcc
     1861 cttccttgct gccaaaatat ttaaaaatcg tcgctttact tacctgagcg ttctcagcta
     1921 ccttatccat tgtcactcca gcggttccta tgtctgtgtt catcaagtca aaagctgctt
     1981 gtaaaatctc tttctttttc tcttgt
//
LOCUS       DAXLPI010000211         2003 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXLPI010000211 DAXLPI010000000
VERSION     DAXLPI010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.220"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..378
                     /locus_tag="ACF3T5_002721"
     CDS             <1..378
                     /locus_tag="ACF3T5_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFD3633224.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /locus_tag="ACF3T5_002722"
     CDS             426..1826
                     /locus_tag="ACF3T5_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFD3633225.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DAXLPI010000212         1992 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXLPI010000212 DAXLPI010000000
VERSION     DAXLPI010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.221"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..258
                     /locus_tag="ACF3T5_002723"
     CDS             <1..258
                     /locus_tag="ACF3T5_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633226.1"
                     /translation="LKRKVTDRNKNLLNVVMICYISFLILGLPQAVFPEMFSGDWGIV
                     SYSILTLIAMVLGTSFYNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            465..653
                     /locus_tag="ACF3T5_002724"
                     /pseudo
     CDS             465..653
                     /locus_tag="ACF3T5_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1365..1970)
                     /locus_tag="ACF3T5_002725"
     CDS             complement(1365..1970)
                     /locus_tag="ACF3T5_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3633227.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
BASE COUNT          647 a          342 c          355 g          648 t
ORIGIN      
        1 ttaaaaagaa aagtgacaga taggaataaa aacttattaa atgtagttat gatctgttat
       61 atttcatttc taatactagg tcttccacaa gcagtgtttc ctgaaatgtt ttctggagac
      121 tggggtattg tcagctattc tatattaaca ttgattgcta tggttttagg tacttcattt
      181 tataatgctt ttgtaagtaa gcatgttgag gaatatgaag tattaatgta cttaaaagag
      241 aaaaaaacaa gtaattaata tgagaacacc acgcatagtt tttattttga gatattctag
      301 aatgaaacac attaagaacg aaagcaaagc aaaagacgcc agaacttcta ctaccttcta
      361 agttttttca tatttgtttg ttatgctata catagatctg cataattaga cttgcgctgc
      421 ccgttattga agtttcaggc agcgctcttt ttgggggatc gataatggaa gaagaaaaaa
      481 tatttgagaa acgctggcag ctagcatcaa gtgaacaaag agcaagatac aataatttga
      541 tcacctccta ccccactgtt gactagacat ataaggaaaa gaaatactta ctttggctgt
      601 gccagttaga tatagatacc tttgagatcc tagataaaat taaacagaat tgatcagaaa
      661 caagcgaagc cttctctcgg ttcgcttgtt ttgctattta ttagttggct cagggcaact
      721 ctaagctcgt ttgaaagaaa accgatggat aatccccttg tttgtaccac tttagaatac
      781 atggatgtac gtattgccat tgactcctat tagttactga aaaaaaattt aaacaagttg
      841 agtattgcag ataaatagct ccagtgaggt gaataactgc tccactcaag gcaatagata
      901 gctccatcca gggcaacaaa ctgctccact ttcatattta atagatgaat aaccttctgt
      961 ataatagaaa ggcatacctt tcggtatgac aacttataca gaatgttatt ttattggttc
     1021 actatcgaaa aattttggag ctttattttt ccggtatttc tcaacgaacg attagtttaa
     1081 ctgtgggaag ctccagacat acgattcgag aagttacttc tcgagcagac aggaaggggg
     1141 tgactgaact aaccgctgat atgagcgata cttggctgta ggaatatctt tttccggaaa
     1201 aacaaacaac agcacgaggt taccttcctg tagactggga atatgttcat aaagaactaa
     1261 tgaaaaagaa tatgacattg aaactccttc atagagaata tggggtacat ggaacataga
     1321 aacgaaaatg cacggtagaa aagcgaatga atatagtata aattctacaa atcattcgct
     1381 ttctttattt tatatatggt tggtcgagag agaccatttt ctttggcaat ttctgcaact
     1441 ggaactcctt gatttaacat gtccaccaca gttttaaaga ctaatcgttt ttgcggatct
     1501 gttgagttct cagaatattc aattggtcgc cccttatatc gaccagcttt tttggccaat
     1561 aatactcctt gccgctgacg ttcacggatt ttctcacgtt cgttttgtgc aatataactt
     1621 agcaatgata aaaacatatc gaacatagca gtatctagga gttcatttcc agtattaaaa
     1681 tctaaaaact gtgcatctag tatttttaat gctacttttt tctgtttcat aaaggtaact
     1741 gtatttttta tgtcttcata atctctgcct aggcgatcca gtgatgcaac aatgacacaa
     1801 tcaccttctc ggatataatt tagtaatttt tgaaactcag gtcgctggat atctttacca
     1861 ctggcttttt cttggaataa tttatcaatg ttaaggtctt ccatcattgc caattgtcga
     1921 tctaaatttt gatcttgtga agacactcta atatatccaa tttttgccat aaaaactcct
     1981 tttaaaaaat ga
//
LOCUS       DAXLPI010000213         1990 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXLPI010000213 DAXLPI010000000
VERSION     DAXLPI010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.222"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..459
                     /locus_tag="ACF3T5_002726"
     CDS             <1..459
                     /locus_tag="ACF3T5_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633228.1"
                     /translation="VYFQGGNLMIKKLFLLSILTLAMAGCSDSGLATELITKSTEATT
                     EELKCGNDHSDYRGLNRRCMGILGRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCAS
                     YTDVEDAVLLNHVRRCSFMSREVGDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(520..885)
                     /locus_tag="ACF3T5_002727"
     CDS             complement(520..885)
                     /locus_tag="ACF3T5_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFD3633229.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(961..1206)
                     /locus_tag="ACF3T5_002728"
     CDS             complement(961..1206)
                     /locus_tag="ACF3T5_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD3633230.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1276..1530)
                     /locus_tag="ACF3T5_002729"
     CDS             complement(1276..1530)
                     /locus_tag="ACF3T5_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633231.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1694..>1990)
                     /locus_tag="ACF3T5_002730"
     CDS             complement(1694..>1990)
                     /locus_tag="ACF3T5_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633232.1"
                     /translation="IAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVITLLLLVMML
                     SYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          728 a          348 c          359 g          555 t
ORIGIN      
        1 gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc aatattaacg
       61 ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac aaaatcaact
      121 gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag aggcttaaat
      181 cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag taatcgaaga
      241 aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt
      301 gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag atgttcattt
      361 atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag
      421 gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt taaagctttt
      481 taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat aaattccttt
      541 ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg gataaatcgg
      601 cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc cacgatacat
      661 aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat ccatgacaag
      721 ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca cagtagcatg
      781 tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg
      841 ttgacgagga agctttgaaa tcaatttttc atgccataat cgcatagaaa tgtctccttt
      901 atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa
      961 tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg aaatatcgta
     1021 aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt cttcgtactt
     1081 acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag ataaagatcg
     1141 gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag cttcttcgat
     1201 actcatttaa tcacctttca ttatagtaga atagttataa tgactataga aagctacaca
     1261 tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa tgacgatcaa
     1321 taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt caaagtagtt
     1381 gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag ctaaataaaa
     1441 ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac
     1501 ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata
     1561 aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata
     1621 gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct
     1681 gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat
     1741 ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg
     1801 aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga
     1861 gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa
     1921 ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata
     1981 aaatcgcaat
//
LOCUS       DAXLPI010000214         1980 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXLPI010000214 DAXLPI010000000
VERSION     DAXLPI010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.223"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..367
                     /locus_tag="ACF3T5_002731"
     CDS             <1..367
                     /locus_tag="ACF3T5_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFD3633233.1"
                     /translation="KKQIVPKEIETWWRIEMQLRRGKATDWHAMVRESLDSFASPHYL
                     PGDVKPVDRIMIKGLNQDHSEWAYISRNLKYRLRKLLKEESQNDELTNHLRETFKESA
                     NDLKIELDTWLLGLDVTEK"
     gene            1331..>1980
                     /locus_tag="ACF3T5_002732"
     CDS             1331..>1980
                     /locus_tag="ACF3T5_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFD3633234.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVDDK
                     VHPLKLSWSIDRITIVGKLNDTIAYWTQNGVVFVDFEQLMRLNENQGYLKSVGTNGWQ
                     LFDQYGEDIAYIEMLKFHPDKGRIDFNPNKIGEFLKGSMKQFIHDLFLDAHFSRADIA
                     CDILGVPDDFITQYRIVDPVSFKPIYGRSGKLETAYWGSRASERQVRMYNKKLEQER"
BASE COUNT          657 a          305 c          461 g          557 t
ORIGIN      
        1 aaaaaaacag attgtaccaa aagaaatcga aacatggtgg cgtatagaaa tgcagctaag
       61 acgtggcaaa gcaacagatt ggcatgcgat ggttcgggaa agtttagata gctttgcaag
      121 tcctcattac ttgcctggag atgtaaaacc tgttgataga attatgataa aagggctgaa
      181 tcaagatcat tcagagtggg cgtatattag cagaaatttg aaatatagat tgcgcaaatt
      241 gttgaaagaa gaaagccaaa acgatgagtt gacgaatcat ttacgagaga cgtttaagga
      301 atctgcaaac gacttgaaaa tagaattaga cacgtggctt ttgggtttgg acgtgacaga
      361 gaagtgatac cgaataagaa aagtcagatg agtgagagta aatttacgtc aatgtttaat
      421 gacgtgagtt tacgataagc gattattttg cgtgaagcaa aataaagcgt aaaaggagtt
      481 gagagcgtga cacgctttcg gctccttttt tcgttgtcac ttgtgacagc gaataccctc
      541 ggagagcatt tggaattgtg acagccatgt cacaattagg aaaatgttta ctctttgtca
      601 aagagtaaca gaaaggttta cactcttttt attagcgaca gcgcgaagga ggggtttaac
      661 ttggggttta gtatttcgtt caaaaaggga acaaagaata caagcatttc tcataataat
      721 cgtgagttaa ctgatagcca aaaaatcaat ggtttgtctg gtagaaaata attagcagat
      781 ttacaagtgg gaaataaaaa gctgaaagaa gaaaattgca tttcaaacta cattttctac
      841 aatagctcta aaatggcata ttttgcgttc tgagcggttt tagcgtttat ttcgtttagt
      901 tatcggcaca atcgttaaaa cacgttagag gagcgaaaat ggagcctgta aggcgactgg
      961 tagcggaacg atcgtgttag attgtgaaaa ccgatagctg aatgaaataa acagcgggtg
     1021 cgccctttcc atttcggcag gaggaggctc aaggggttcg gggaatgaaa ttccctgagg
     1081 tctttttatt tttttaggag gagtgctata atcaaagaaa acgagaaaaa agagcaaaat
     1141 aaaagccgag cagtgcgcta acactctcga ctatttacgg aaactacttc acctagctcc
     1201 gtaactcagg ttcattatac gaatattttt aggtttcttc aagctagaga tttttggata
     1261 tgaattttta gggttacgtg aactctggaa attctttcca aaagtctctt ttttcgtctg
     1321 gaggacgaaa atggcaaatg aaaatcagtt gagtgaaatt caatcaaaaa ttgaaggtcg
     1381 aggggcagaa aaaagaacaa cgataaatca gctcgaaaaa cttaaccgta tttctaacag
     1441 gcggttaagt gttgacgata aggtacaccc cctaaaatta agttggtcaa tcgatcgaat
     1501 tacaatcgtc ggaaaactga atgataccat agcttattgg acgcaaaatg gagttgtgtt
     1561 tgtcgatttt gagcaattaa tgcgtttgaa tgaaaatcaa gggtatttga agtcggttgg
     1621 tacaaatggt tggcaattat ttgaccaata tggagaagat atcgcctata tcgaaatgtt
     1681 aaaatttcac cctgacaaag gtcgtattga ttttaatcca aataaaattg gcgagttttt
     1741 aaaggggtcg atgaaacaat tcattcatga tttgtttctg gatgcgcatt tctcacgtgc
     1801 agatattgct tgcgatattc tcggtgttcc tgatgatttt attacccagt atcgaatcgt
     1861 tgaccctgtg tcttttaagc caatttatgg ccgttctggc aaattagaaa cagcgtactg
     1921 gggtagtcgt gccagcgagc gacaagtgcg gatgtacaac aaaaagcttg aacaggaacg
//
LOCUS       DAXLPI010000215         1971 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXLPI010000215 DAXLPI010000000
VERSION     DAXLPI010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.224"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            192..425
                     /locus_tag="ACF3T5_002733"
     CDS             192..425
                     /locus_tag="ACF3T5_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFD3633235.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            422..829
                     /locus_tag="ACF3T5_002734"
     CDS             422..829
                     /locus_tag="ACF3T5_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFD3633236.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1280..1789)
                     /locus_tag="ACF3T5_002735"
     CDS             complement(1280..1789)
                     /locus_tag="ACF3T5_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633237.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT          680 a          301 c          326 g          664 t
ORIGIN      
        1 aatttaaaat aaatggtata cataaatagg gaaggaaata gaagggagca atctaaattt
       61 tttgaatttt cttaattaat tatatagaaa gaacaatctt tctatagagt tagacatgag
      121 agggactatg cttgatttaa aatcaacaag cgtatatact gtatatacaa agaaaaagga
      181 gggagaagac tatggcaatt aaagaaagaa aatttaggga agtggggaac tcagtagtat
      241 taactatttc aaaggagttt cttaaagaaa atgggttaaa tcccggagat acggtttttt
      301 tagatgaaga aaaacttcga gcggcaatta caaaaaaaca agccttatct gatgaaattg
      361 atttagcaat atctcaagca ttaactgaat atgatactac ttttagagag cttgtcgata
      421 aatgagtatt cattacctag aagccaaaga tattataaaa atgaatgtaa tccaaataaa
      481 aaaatactct cctaatgaac cccttggtgt aaaggatgcg aatgctttag aaatgtgtgt
      541 agggcaactt cgagcctcag cttttggtga agaggtttat ccttcgattt atgaaaaagc
      601 agctatccta cttatacaat tgataaagaa acatcctttt cacaatgcaa ataaacgtac
      661 tgctttctta gctacttttg tctttttgaa aatcaatgga cagcttttag ctattgatca
      721 aaaagatgct gtaaatttag cggtatatat tgctacgtat gataaggatt tcgatcaact
      781 aaaatatgaa gtgatcaaca ctataaagac acatacgcag cccatttaag gagttactgt
      841 atgtgtcttt atagggttat tagctgctat agcgaaataa ttgaagtaaa ttacaaatag
      901 ctataattca aatccaacta tatagaaaac gtaaattttc tatatacttg gatatgctgg
      961 aatgtttaag agaataatta catttctaga aaatacaata aaaattacag gataattagc
     1021 catatcctac ctcctctagc agttgctcaa aagttagcac taagtaactt cttttttgtt
     1081 gctggtaaca atttatcaat actaaaatag cgtttaagta tctaataaat actaatttta
     1141 aagctcttaa ttattatgaa acacgatata acaatattgc tatatcgtgc tattgtaata
     1201 ttatacattt gaagacagcg tattcttgtt ctaatatcag gaatttttta taaatatcag
     1261 tcatattaag actcaaaatt tattggtatg ttcctctgta tagagtttga ccgtctacca
     1321 ttatatagca attattatca ttgttgtctt gcaccaatat accgttaagg gtagcactag
     1381 gagtagtaac aatcactgga aactcatgag aaagctcggt catttttcct gttctgctag
     1441 aatcgtgttc tattgtcaag ccatttacta taaacgtgaa tactttactt cctattgtta
     1501 tagtagcttt ttgagaaaca gtttctgtag ctggagctgt gcttctttcc gaatttgtta
     1561 gggtttcact ggattcattg ctataaacaa ttgtagagga ttctacggct tcttgagtgt
     1621 ccacgttggt gctgctagct tcatctagct tccttttgta ttgttcgtta ctgattgcta
     1681 agctttcccg ttggtcatca agcttattca tttctgtaat ggtataaaat aatcctcctg
     1741 ctagtgctat attaacaact aaactaaccg caaataatac ctttttcatt cttccttctc
     1801 ttctttctct ttttgtaatt ccttactttt ggataggcta caattaacta gacagaaaaa
     1861 ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct aagagaacac
     1921 gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta g
//
LOCUS       DAXLPI010000216         1968 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXLPI010000216 DAXLPI010000000
VERSION     DAXLPI010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.225"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            316..1476
                     /locus_tag="ACF3T5_002736"
     CDS             316..1476
                     /locus_tag="ACF3T5_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFD3633238.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTIAEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            1492..1935
                     /locus_tag="ACF3T5_002737"
     CDS             1492..1935
                     /locus_tag="ACF3T5_002737"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3633239.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
BASE COUNT          683 a          331 c          389 g          565 t
ORIGIN      
        1 ggtgttcaat cagctgcttc tcggtaataa gctcactagc ctagaaaata tgagaagcta
       61 ttttctgact agcactctta ttactcaaag cataacgcag ttttcacaac ctcttcctta
      121 aacggtgttc aaaagcttgc atctgcggta ataagcccaa cccaacaaaa aatatgagta
      181 gctatttttt gaggctgtac ttattacttg atgcaggaca gctttttcac aatctcatcg
      241 gctttcattt tctatttttt tcctgaaccc atataataaa agaaagaaca aaaaaacttg
      301 caagggagga cacatgtgaa gcagagtaag caaaaacagt ctcgtcgact tttgtttcgt
      361 ttgtttttat taatatgtat cgtcgtaggg ttactgatcg ctttatatcc attttatgtg
      421 gattctttaa atagttttat tgatcaaaaa cgtatcgaac aagtacaaaa acggaccgca
      481 gctgaaattg aagtgcagcg aaagaaaatg gaggagaaaa accaacgatt aactgaccaa
      541 ggattcaatc ctggtactga tcctttcaat gaaaagaaca gaaatgaaag tacaacatcc
      601 agccaattag aggaatggct gatcggaagt atcaatatac ctaagatcca attaaatatt
      661 tcactttatg atcggctcaa tggtaggatc cttgaaaatg gagcaggcgt cttgcaagga
      721 acctctttcc cgcttggtgg aaattcgacg cattcagtga tctctgctca cagcggttta
      781 cctaatcgac ggttatttac cgaattagat cgtttagaaa acggagacac gtttattttg
      841 actgttttgg gagagaaatt ggcttatcaa gttaaaaata tacaagtggt actgccagat
      901 gacacgtctg ttctaacgat tgcagaagga gaagacttag ttaccttgct tacatgtacg
      961 ccgtatatga tcaacacgca tcgtttgcta gtgacaggac atcggattcc ttataacgaa
     1021 tcggtaaaaa aagaagaagg aaaaggaaat caggaaagaa ccatgcggca atggtttatt
     1081 cttgcaggaa cgataatagc tgtagtcata ctgttagtgt ttatcggacg actaatctat
     1141 cagtatagat taagtaaaaa agtgcttgat ttttcattta ttattagtga ttcgtcaggg
     1201 aaaccggtaa atggtagttc attcattttg aaacataaga aaaaaacact tactagaaat
     1261 ggtgtgccgt tttcagtaca aagcgaccat catgggaaag tcaagctgga tcaattgcct
     1321 ggtgggactt atcgaatcgt ttcagctgat gatcctaaag ttgctgtttc ttttggtatt
     1381 agaaaagtaa aacaagagaa gatatacttt tttgaggggc gaaaacttgt aaaagagctt
     1441 caaaaaaatg gtttttattt taaaatcaat gaataagtaa ggagagaaaa aatgatccgc
     1501 acaacaacaa aattgtctgc caaagaactg gaatctgttc tatcgatttg gctacatgct
     1561 aacttggatt ctcatgaatt cattccatca acttattggg agaatcaagt agaatctgtt
     1621 aggaaagctt taccagatgc agtgatttac ttagcagaaa tagatcaaga aattattggt
     1681 tttgctgggg tgattgatca gtttatcgca ggaatctttg tgaaaaggga ataccggcaa
     1741 caaagaatcg gtacgcaact aatgaaaaaa ataaaagaaa ccaataaaac actgactttg
     1801 acagtctatc aaaaaaatca atcagctatt catttctatc tttcacaagg ttttacaatt
     1861 caaacacagc aaaaagatga agcagtaaat gaggaggaac ttgtgatgaa atggaatcaa
     1921 aacgctgatt tttgatgaac agaataacga aataatgctc ccagccta
//
LOCUS       DAXLPI010000217         1955 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXLPI010000217 DAXLPI010000000
VERSION     DAXLPI010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.226"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            11..1189
                     /locus_tag="ACF3T5_002738"
     CDS             11..1189
                     /locus_tag="ACF3T5_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFD3633240.1"
                     /translation="MENMSRFKKHITATGKITWYFQAFRTTRRGFNSKREAQLAYLEM
                     EKQHRNKQKRIASNDKFNVVSQKWFEYYRSLNEQKRATYDKREEQVKILNRWIGDKKL
                     SSLSSEELQKFLFILKEKGIDGIDVGYAKNSLQSLVQVLNMIFKYCKKKNLINENPME
                     FVKMPKYQLTVEDLKESVNSLEDKFLTVDELRTFLNYGIIYEELPMSVLFHVLFYTGC
                     RVSEALALQPQDIDFERNEILFYKQTAVKGKSKDFRIETTKSVSSARRVPVTPLVMEK
                     LHELIDVLNKTKRNSNFVVDETYLFVYLDPGKRGVPYRREYVNDHVKRCVERCGINKN
                     FHTHLARHTMASLVAEYCSWDVLKDRLGHTDSTTSKIYRHLTSNERIKPLLAFDSLEN
                     "
     gene            complement(1368..1553)
                     /locus_tag="ACF3T5_002739"
     CDS             complement(1368..1553)
                     /locus_tag="ACF3T5_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633241.1"
                     /translation="MLEYSGFFIKAVTLKKDIIIVLFFLLLLIGRFILIHYSPLPIIK
                     EKTGSGCSNYMLRKKNP"
     gene            1590..1883
                     /locus_tag="ACF3T5_002740"
     CDS             1590..1883
                     /locus_tag="ACF3T5_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFD3633242.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT          726 a          249 c          367 g          613 t
ORIGIN      
        1 gtaaaaacaa atggaaaata tgagtcgttt taaaaaacat attactgcaa caggaaaaat
       61 tacttggtac tttcaggctt ttcgaactac gagacgaggt tttaattcta agcgtgaggc
      121 tcagctagct tatcttgaaa tggaaaaaca acacagaaat aagcaaaaaa gaattgctag
      181 taatgataaa ttcaatgtgg ttagtcaaaa gtggtttgaa tattatcgat cactaaacga
      241 acaaaagagg gcaacatatg ataagcgaga agaacaggta aaaatactta atcgatggat
      301 tggtgataaa aaattaagca gtttatcttc agaagaacta caaaaattcc tttttatttt
      361 aaaagaaaaa ggaattgacg gcattgatgt tggttatgct aaaaatagtt tacaatctct
      421 tgttcaagtt ctaaatatga tttttaaata ttgtaagaaa aagaatttga taaatgaaaa
      481 tccaatggag tttgtaaaaa tgcctaagta tcagttaaca gttgaagatt taaaagagtc
      541 tgttaatagt ttagaagata agtttttaac agtagatgaa ttgcgaactt ttttaaatta
      601 tgggatcatt tatgaagaat tacccatgtc tgttcttttt catgttttgt tttatactgg
      661 gtgtcgtgtg agtgaagcac ttgctttaca acctcaagat atagattttg aacgaaatga
      721 aatcttattt tataagcaga cggcagtaaa aggaaaaagc aaagattttc gaattgaaac
      781 tacaaaatct gttagttctg ctagaagagt acctgtcaca ccgttagtaa tggagaaatt
      841 gcacgaatta attgatgtat taaataaaac aaaaagaaat agcaattttg ttgtagacga
      901 gacttattta tttgtttatc ttgatcctgg taagcgtgga gttccttatc gtcgtgaata
      961 tgtaaatgac cacgttaagc gctgtgtaga gagatgtgga atcaacaaaa atttccatac
     1021 tcatctggct cgtcatacga tggctagttt agtagctgag tattgcagtt gggatgtttt
     1081 aaaggatcgt ttaggtcata cagatagtac aacttcaaaa atatatcggc atttaacatc
     1141 aaacgaaaga ataaagcctt tgcttgcttt tgattcatta gaaaattaga ttttggcaga
     1201 agtgcaggta ttttagaatc taaaataggt gttgtgacta tgctttagtc aaagtgtagt
     1261 cagaaaaaga gaaaaacttt tatatcaata ctttaatgac ttttcacaag tatcaaagaa
     1321 atccgtcgtt aatcattgtt atcagactga gaaagaaccc ttggtattca agggttcttt
     1381 tttcttagca tgtaatttga acaccctgat ccagtttttt ctttaattat tggtagggga
     1441 gaataatgga ttagaataaa tctacctata agtagtagta aaaaaaagag cactatgata
     1501 atatctttct taagagtaac cgctttaata aaaaatccag aatattctaa cataaatgga
     1561 gtattcttag aatcaaaagg agaaagaaga tggtttatac ttatacggag caagaaaaga
     1621 tgaaagtttt agacacattc atgaatgatg gaaaagtaaa acaagtacca agttcaacaa
     1681 aaaagaaata catcctattg aaagaagtct tgaaacgctt tgaccatgga gtgacttata
     1741 cagaaacaga ggttaattcg atactgctga acgtattttc tagtggcgat tatgtcgaac
     1801 aaagacgtta tctaatcaca tttggctttt tcaaaagaag ttcagacggt cgggcatatc
     1861 agatgatggg aattgaaaat tagaagtttt aatttatact cattgaaaaa agttcaagta
     1921 tgcgatattg gttctacacc gcatacttga acttt
//
LOCUS       DAXLPI010000218         1915 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXLPI010000218 DAXLPI010000000
VERSION     DAXLPI010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.227"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            108..1253
                     /gene="tgt"
                     /locus_tag="ACF3T5_002741"
     CDS             108..1253
                     /gene="tgt"
                     /locus_tag="ACF3T5_002741"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFD3633243.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1350..1730
                     /gene="yajC"
                     /locus_tag="ACF3T5_002742"
     CDS             1350..1730
                     /gene="yajC"
                     /locus_tag="ACF3T5_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFD3633244.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
BASE COUNT          662 a          319 c          414 g          520 t
ORIGIN      
        1 aatattaaat aaagtagaag gaagccgagg gaacttgcaa aaagcacttg aaacgattat
       61 gatggtggga gttgtcaaga tattttatat agaaagtagg gaaatgaatg acgcaacctg
      121 ccattcgcta tcgtttaatc aagaaagaaa aacatactgg cgcgcgttta ggggaattga
      181 tcacgccaca cggaactttc cccacaccaa tgttcatgcc agtaggaaca ttagctacag
      241 taaaaacaat gtccccagaa gagttgaaag aaatgggtgc tggcgttatt ttaagtaata
      301 catatcattt atggctacgc cctggtgaag acttagtaga agaagctggc ggactgcata
      361 aatttatgaa ctgggatcaa ccgattttaa cagattcagg cggctttcaa gttttttcat
      421 taagtgatat gcggaatatc gaagaagaag gcgtgcattt caaaaatcac ttgaacggtt
      481 cgcgtatgtt tttatcacca gaaaaagcaa tcaacatcca aaataaattg ggctctgata
      541 tcatgatgag ttttgacgaa tgtccaccat tcgatgaaag ttacgactac gtgaaaaaat
      601 cagtagaacg tacgtctcga tgggcagaac gaggattgaa agctcatgct aatccagacc
      661 gtcaaggatt atttggaatc atccaaggag cagggtttga agatcttcgt cgtcaaagcg
      721 cgaaagatct agtcgggatg gatttccctg gttactcaat cggcgggtta tctgtcgggg
      781 aacctaaatc agaaatgaat cgtgtattag aattcactac accgttgatc cccgaaaaca
      841 aaccacgtta cttgatgggt gtcggtgctg ctgattcatt gatcgatggt gttatccgtg
      901 gagtagacat gtttgactgt gtcttgccta ctcgaatcgc tcgaaatggc acatgtatga
      961 cttctcaagg ccgtctagta gtgaaaaatg cgaaatatgc ccatgatttc cgtccaattg
     1021 atgagaaatg tgattgttat acatgtaaaa actatacacg tgcctatatc cgccatttga
     1081 tcaaatgtga tgaaacattt gggattcgtt tgacttctta ccacaattta tacttcttgt
     1141 tgaatctaat gaaacaagta cgtcaagcaa tcatggatga taatttgctt gaatttagag
     1201 aagccttttt tgaagaatac gggttcaata aagaaaacgc aaaaagcttc taaaggaaaa
     1261 tcttttaaaa attaagtaaa aaactagtgt tatcagcatt tttctgctaa agtgtttttt
     1321 gtagatacag aattagtgag gtgaaaaaaa tgggaacatt accaatgctt gtgatgttta
     1381 tcgcgctatt agcaatgtgg ttttttatgt cacgttcgca aaagaaacaa cagcaagaac
     1441 gtcaaaactt gttagatagc atgaaaccag gtgattcagt cgtaacgatc ggcggattac
     1501 atggagttgt ttcagaaatc gacaatgaca aacggacagt tgttttagac tgcgaaggga
     1561 tcttccttga gtacgatcgt gcagcaatca aaacggtcaa accagggact gtcgtaacaa
     1621 atgatgcaac gactactgtt gaaacaacag aagaaccagc tgagacagtt gaagtaaaaa
     1681 ctgaaacaac agaaacgctg gaagagacag aagagaagga agaaaaataa gtatcaaagc
     1741 ttatttaaag acgaggtagg gcagaaagat gtcctacctc attttttatt caaaaaagaa
     1801 agagataaga gcatgggata tattgctcaa tattgaataa tcgggtgtgt catataaata
     1861 ataataaata actttatata tttttctata caaacacctg taaataaagc aattt
//
LOCUS       DAXLPI010000219         1913 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXLPI010000219 DAXLPI010000000
VERSION     DAXLPI010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.228"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="ACF3T5_002743"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="ACF3T5_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFD3633245.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="ACF3T5_002744"
     CDS             852..1040
                     /locus_tag="ACF3T5_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFD3633246.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="ACF3T5_002745"
     CDS             1053..1610
                     /locus_tag="ACF3T5_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFD3633247.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="ACF3T5_002746"
     CDS             1642..1884
                     /locus_tag="ACF3T5_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFD3633248.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          702 a          295 c          393 g          523 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aat
//
LOCUS       DAXLPI010000220         1810 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXLPI010000220 DAXLPI010000000
VERSION     DAXLPI010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.229"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..1809
                     /locus_tag="ACF3T5_002747"
     CDS             <1..1809
                     /locus_tag="ACF3T5_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633249.1"
                     /translation="SFYLRIKWSIIIILSLLFLTGIAYFFYFIFSPADSNQMKEIKDS
                     WVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYL
                     GEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTT
                     GPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGF
                     EAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLI
                     FIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLV
                     AIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKI
                     KNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNF
                     EMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHS
                     FRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLM
                     MMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT          585 a          338 c          305 g          582 t
ORIGIN      
        1 tctttttatt tacgtataaa gtggagcata atcattatat tatcattgtt atttttaaca
       61 ggaattgctt atttctttta ttttattttt tctcctgcgg acagcaatca aatgaaagaa
      121 ataaaagatt cgtgggtttt ctatacaaaa gatgatcctg attttaagtt tgacagcaag
      181 tacactcgta cgattcctac cgtggataaa gacgaaacat tcattatgga aacgacactt
      241 gaaaaaccgt tggacgaagc aaatttacta atcaaaggaa accatcaatg gattactgta
      301 tatctaggcg aaaagatttt ataccaacgg aaagattatc aggaagaaag caatcccgga
      361 ctctcattgg ctgtgataga tttaccagca aattatatcg gaaaaaaatt gacccttaca
      421 gtctcatctc cttatcagaa ctatgctgga ctgccaccga aagtttatct aggaacaact
      481 ggaccattga ttagttttat ttattcacaa tcagtgccgc aagtattgac gatgataatc
      541 gctgttttta tcgctatagg gacctttttc tatacgatct acttgatgta taaacaaaaa
      601 cgtcttgaat actcacttat tctcttaagt tgctttgctt tggcattagg atttgaagct
      661 gtatcagaag atattttaag tggtattctt tttgaaccat tcgttcattc ttttttatct
      721 catttattta ttatcttaac gtcagctttc atcatctgct actattggag ccgaatgttt
      781 tactataaaa aatggtacgg aattttttgt acgttccaac ttttgatttt tatcggcgtt
      841 ctgctttatg caacttttac tcctgcagaa cttcctgaat taatgccgat tgctaatatt
      901 gctagtgtta taagcacttt agtcacttca cttgcatgca ttggcgaagc atataagaat
      961 aaccgttttt atgtagcttg tacaccttgg atcgtcttgg tagccattat ccattgcttg
     1021 atttacatcc aaacggcatt aggtatctat caaacaacga tcaattggtc tacgatttta
     1081 tatattatca ttctaatcgt tattatcagc tacaatctaa tcgatcacat cttaaatacc
     1141 gacaaagatc ggagacaagt agatttctta aaaatcaaaa atgatttgtt ggagcaacat
     1201 tatgagcagc ttcgacagca tatccaagaa gtaaatacac ttcgcctgga atttgtccaa
     1261 gagatggaga atctgcaaga tttaatgcat acggaccaaa caaaagctaa aaactatgtg
     1321 gaaaagatat tagaagaagc aaaaaatttc gaaatgctgt gttctttttg caaccaccag
     1381 gtgacaaatc ttatcttagc gcgttaccag aagttagctg ccaaacggaa tattcagata
     1441 accttccaag cagatcttcc cgaagaactt cctatcaacg atgacgatct ggctcaactt
     1501 ttgatccacg ctttagagca ttcctttaga gaaactcatg ccatcgaaaa tccactgtat
     1561 cgaaaaattt atttgtcgat acacgaacaa gaggaacatt tcgtcatttg ttgcgaacat
     1621 agtgcacatt acgatacaaa cctatttagt cgaggcatca cagaagaatt agatgatcag
     1681 gaacgttttg acttaatgat gatgaaagac gtagcggatc gttacacagg aacactaacg
     1741 caggaaaaag atactttcat tgatcggatc actgttcaac tctctcgaaa ttacccgaat
     1801 cctgtataaa
//
LOCUS       DAXLPI010000221         1802 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXLPI010000221 DAXLPI010000000
VERSION     DAXLPI010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.230"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            223..819
                     /locus_tag="ACF3T5_002748"
     CDS             223..819
                     /locus_tag="ACF3T5_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFD3633250.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(877..1359)
                     /locus_tag="ACF3T5_002749"
     CDS             complement(877..1359)
                     /locus_tag="ACF3T5_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633251.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1465..1662)
                     /locus_tag="ACF3T5_002750"
     CDS             complement(1465..1662)
                     /locus_tag="ACF3T5_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633252.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
BASE COUNT          606 a          364 c          273 g          559 t
ORIGIN      
        1 tatcagctgt ttttttcaaa taagcaaaaa ttcctaaaac acaacaatga aagcgataac
       61 taagaaaatc cttacataac gatttttctt attaaaattg ttttttgaac ttcgcttatt
      121 ttttgtgagc atatctgttt acttactttt tgtaaggtgc tatacttcat ttcgtaatca
      181 attaaaaaaa tgcaaaacgc aaatcaaagg agaaatttta ttatgacaac ttttactgac
      241 actttgaaaa accgccgttc aatctatcat ctaggacgta atgtttcact ttctaatgaa
      301 gaattaacaa ctttgatcaa agaagcaatt aaagaaagcc caactgcttt caatgctcaa
      361 tcaacacgcg cagttatcct tttcggagat gcacatgaaa aactttggga aatcactgaa
      421 gaagcacttc gcccattgac tccagcagaa gctttcccaa atacacaaaa caaattagct
      481 ggtttcaaaa atggttatgg tacagtatta ttcttcaaag atactgacgt ggtaaaaggt
      541 ctacaagaac aatttgaatt atatgcagac aacttcccag attggtcaga acaatcaaac
      601 ggaatcgcaa cagctaatac ttgggtagca ttagttgata aaggattggg cgcaaacttg
      661 caacattaca acccagttat tgacgaagca gttgcaaaag aatggaatat cccttcaaac
      721 tggaaactac gttcgcaatt agtattcggt tcaccagaaa caccggctgg ggaaaaagaa
      781 tacatgaacg atgccgatcg cttccgcgta tttggataat tttatttgaa ataaaaaaca
      841 gaggttgtga caaacatctt gtcgcaacct ctgtttttat tctggatcaa tcataatatc
      901 aatctctgcc tcatcgttta agcgttggga caaaaatgga actaattctt ctaatgatgc
      961 agaaaatttc agcatcggtc ctcgccatcc gtttcggaaa aaattcattt ttacgatttt
     1021 agaacctttt ggctgatata atacaatttt attgatctca gaaaaaggaa cacctttttt
     1081 gtcaaagcta ttcaaaacaa gtccttcttc tgtgattcct ctactgtcca acaaaaaact
     1141 aataaaaatc attgctgaca agatcccttt tgccaattca tctgctgtag gatgactgat
     1201 gagtgtatac caaatgacta gtgacactaa gaagggtatc accaagtaga cccatcgatt
     1261 tcgcacagtc cgataaataa tttcttttcg aaccagccaa agaaataaaa acatcacaac
     1321 aacgaataaa aacaatagaa ccgtaaagaa tgttaccatg acaactcgcc tcgctttttc
     1381 ttctccagaa aataggaaaa gaatactgat ttaccttctc aaagaaattg gacttgcagc
     1441 acgccgcacg tccaattgtt ttctttattc aaatgtatca tattttgcaa caaccatgct
     1501 tgctttttca tctaataatt cttggtcctc tgcagcacat tgggcaactt tgaccacggc
     1561 gcagtttgtc atttttgata caacttcacc aatgaccgtt tttgtaaaac caattggttt
     1621 gcatgcatat tgttctccta aattaatcat ttcttccgtc atcaaaatcc tcctaaactg
     1681 ctggaacttt tctccacatt accactgcta tttctttgga gatttaactc attgattttt
     1741 atttacagtt aaaaatcaac cttacaggca ttatagcgta ctgttttcgt tttcacaatt
     1801 aa
//
LOCUS       DAXLPI010000222         1791 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXLPI010000222 DAXLPI010000000
VERSION     DAXLPI010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.231"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            21..830
                     /locus_tag="ACF3T5_002751"
     CDS             21..830
                     /locus_tag="ACF3T5_002751"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFD3633253.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            870..1367
                     /locus_tag="ACF3T5_002752"
     CDS             870..1367
                     /locus_tag="ACF3T5_002752"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFD3633254.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT          621 a          322 c          342 g          506 t
ORIGIN      
        1 aaagaaagta ggaagataaa atgaaactag cagcgattga tttagacggt accttgcttg
       61 atcgaaatgg aaagatacca gaagaaaata tccaagcact tcggaaattt gatgaaaatg
      121 gcggaatcgt aaccgtcgct accggaagga acagcatttc tgctaaagat attttttccg
      181 aattaggaat cggtggctat ttgatttctt ccaatggttc actgattgct gaaatgaagg
      241 atggaaaaat ggcgaaagtt ctcagacgtt caaaaataga tagcgataca ctgagaaaag
      301 ccttcttcta tgctcaagaa gcgaaaatat ccatcattgc tagccgagaa acccaagatg
      361 atcagctgac attcgatgaa tcaaatctag tcaaagatga cccttattat gaacatttca
      421 atttacagaa ccattctttt gatcaaattt taaatatgtt ggaagatcct tctcttagct
      481 atcttaaatt agcattaaca gataagagtg aagaaaaact acagaagcta caaagagatc
      541 tgcaagcaga tggtatccaa tcagtattct ctgatccgca tttcttagaa atcacgccta
      601 caggtatcac taaagctcat tcacttgttt ttctggcgga ttaccttgga atcgaatcga
      661 gtgacatcat ggcttttggc gatcaagaaa acgacattgc tatgttgaat tacagcgggt
      721 tgagtattgc gatggggaat gcacagcgac aagtaaaaga aattgcaaac caagtaacac
      781 tgacgaatga agaagctggt gttgcttata ttttaaaaaa cttctattaa aaagaataaa
      841 atgacgatat gaatgtagga ggaaagaaaa tgttagttat tattgatatg caaaatcata
      901 ttcttgatcc aaacagcgaa ttttatattc cagagtcaga tcaattggca gaacgtattg
      961 ctcaaagatt agctaaagca cgccaaaata atgaatatat tctcttcaca cgggatatac
     1021 cagttgaact gaaggacaaa gaagaagaac gagaagattt gcaattgatc cctcttcttt
     1081 ctccacaaga aaatgaacga gtgattaaaa agtattactt tacgattcca cctgaaactt
     1141 tagctgaaat ccaggaaact ttatttgaaa gcaaagaaga acaaaaagag atcgaagtag
     1201 tcgggattga aacaaacttg tgtgtactct ctaatctgat aggcctgcag agtgcttttc
     1261 ctgaagcaga cttttttgtt gatccaatgt tagtctccag tagaaaacac ggagaatccg
     1321 ctttagagct gttgaaagat ttcaacgtct ctatcgtcga atcataaaaa tatctatctt
     1381 catttacgat atttaacaca caataaaaaa agcacgcaga actgttttag tcctacgtgc
     1441 tttttctatc aaagtagatt ggctttttta tgacacagag tgaaacggca ggctcacgac
     1501 ctctttctta aatggtgttc aatcagctga ccctcggtat tagcttaaca aacggcaaaa
     1561 tatgaggaac tatttttccg tttgttaagc taatactcag gtcaaaacgc tgatttcaca
     1621 acctcttcct taaacggtat tcaaaagctt gcatctgcag taataagaac agccaagtaa
     1681 aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg cagctttttc
     1741 acaacctctt atgtaccttg taggactcga acctacgacc ggacggttat g
//
LOCUS       DAXLPI010000223         1775 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXLPI010000223 DAXLPI010000000
VERSION     DAXLPI010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.232"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            125..739
                     /locus_tag="ACF3T5_002753"
     CDS             125..739
                     /locus_tag="ACF3T5_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3633255.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(904..>1775)
                     /locus_tag="ACF3T5_002754"
     CDS             complement(904..>1775)
                     /locus_tag="ACF3T5_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633256.1"
                     /translation="FYSKEKLADFSNIAEMIERLKDFMISVWDVAMLADESNYTSSIY
                     RLEDSNWIEISLELKTLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNI
                     SNTRDRFISIITACEIGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFP
                     KEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFY
                     EESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFV
                     ENIDKEPTNTVGD"
BASE COUNT          595 a          301 c          271 g          608 t
ORIGIN      
        1 ttaaattttc ttgattgaca tttatttaca aaaaatattg ccatttaaaa agaaaagtga
       61 catatggctt cctcgtgtat gattagggtg tattttaaat agttgcagaa agtaggtaaa
      121 agttttgaga gttgcataca tacgtgtctc atcgatcgat caaaatgaac aacgccagat
      181 tgaagaaatg aaaaaattcg gtgcagagag aattttcatc gagaaacaat ctggcgcaac
      241 gattactcat cgacctgttt tccaagaagc attagattac gtccgagatc aagatatttt
      301 tattgtggaa gctattgatc gacttggaag aaattatgac gagatcatcg cttcagtcaa
      361 ttacttgaag aagaaaaatg tgaagctgat cattactagt ttaccgatca tggctgaagc
      421 aattggcaat cctctcctgg ataaattcat taaggatctg atcatccaaa ttttggccat
      481 gatcgctgaa caagaacgga ctgaatcgaa acgtcgccag gcacaaggaa ttaagatcgc
      541 aaaagcaaac ggcgtctaca aagggcggcc caaattatac agtgcagacg caaaagatcc
      601 ccaacgtcgc cttgtttaca aaagcatcgt tgaagatctt aaaaatggtg ttgcgattgc
      661 taagattgct aaagactaca atgttacgag acaaaccgtg tatagaataa aaaaagacag
      721 catggtcaat gacaaatagt tgatcatgct gcttttatta tcttctcatc tttaatttcc
      781 tacatataat tactatctat aaaattaagt cttactcaga acagataaag cataaaattc
      841 caacaaacca ttttttactt aactaaataa agagtctgtt aatataatct tcacttattt
      901 acgttaatcc cctactgtat ttgttggttc cttatctata ttttctacaa aatccttatt
      961 atcttgtgat ttagcttcgt ataattcttt gtatgtcgac acattggtta tactaattcc
     1021 tatagctagt tcttgagact ctacttttct ctctagctcc tttgacaaag tgatttgaga
     1081 tttatttggt tccgttggag tcaattctat agattcttca taaaaatctc tatacataaa
     1141 gctatcatta attttataaa aaaaattaaa tgtttttagc actgccatat aacattcaaa
     1201 acattcttct atagttggtt tacttccttg aggagaatga accaatttat tacgtttttc
     1261 tatgaaattt ttaatttcct ttcttatttc tttcggaaac tcaatattaa agtacccttt
     1321 aaacatgctc cctaataatt tgtctacagg gggagattgt aaattctcaa atattagagt
     1381 taaatcaggt ttctcttgtt gataaaattc ctttacccct atttcacagg ctgtaattat
     1441 cgaaataaac ctatctctag tatttgatat atttttactt cttttaagga gtataagaga
     1501 aaaggggata ctttcgtgtc catgcatgta attaactaat tgttgagctt cagaagttat
     1561 atctctaatt atatagttaa cggaattagg aagtgtcttt aactctaatg aaatttctat
     1621 ccaattacta tcttctaatc tgtatatgct cgaagtataa tttgattcgt cagcaagcat
     1681 cgctacatcc caaacactga tcataaaatc ttttaaacgt tcaatcattt cagcaatatt
     1741 gctaaaatca gctaattttt ctttagaata aaaaa
//
LOCUS       DAXLPI010000224         1765 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXLPI010000224 DAXLPI010000000
VERSION     DAXLPI010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.233"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(430..1002)
                     /locus_tag="ACF3T5_002755"
     CDS             complement(430..1002)
                     /locus_tag="ACF3T5_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3633257.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGIKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1018..1623)
                     /gene="fic"
                     /locus_tag="ACF3T5_002756"
     CDS             complement(1018..1623)
                     /gene="fic"
                     /locus_tag="ACF3T5_002756"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFD3633258.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          517 a          361 c          259 g          628 t
ORIGIN      
        1 taatatcaca ataggaatgc tgtttattat attagcagca gtgctgattt ttatgatagt
       61 tatttggttc ttaaatgaaa gatctaaaga caatcaacac cgtagggata tggagataaa
      121 atcaatagag tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac
      181 gtgacttacg ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata
      241 tatgatggta tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta
      301 cagttaccat acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac
      361 aacatgctaa tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag
      421 acttcttttt taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa
      481 accggttata gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg
      541 cctcttcttc ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt
      601 tcttgcaaac atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc
      661 tacactcaac aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag
      721 attcaaaacc ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc
      781 tctcgtgtta cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag
      841 accagccaac agctgattga attttggccg atcccgtttt atacccgtaa atttttctga
      901 aaaaatattc ttcttctcta cgccgttctc aagcaactgt tgaatttgtt cttttgtact
      961 ttgagaagcg gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta
     1021 caaatcatct atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt
     1081 catatataac tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat
     1141 ttccaaatcg tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa
     1201 gttccaatct acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt
     1261 acttcggcca tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat
     1321 aatctcttca aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata
     1381 cataactggt gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc
     1441 aaaatcgtaa atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc
     1501 aacttcgatt tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag
     1561 tttttcttca accttagcca actcaacttg attggttaat cccaatttat tctctaaaat
     1621 catgcgaaat ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata
     1681 tcgcgatttt gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa
     1741 gtgtctctat tttgcacagt ctagt
//
LOCUS       DAXLPI010000225         1762 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXLPI010000225 DAXLPI010000000
VERSION     DAXLPI010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.234"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..313
                     /locus_tag="ACF3T5_002757"
     CDS             <1..313
                     /locus_tag="ACF3T5_002757"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3633259.1"
                     /translation="YQDDKCVGKVKLRKNWNRYAYIEDIAVCKDFRGQGIGSALINIS
                     IEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDTMLYANFENNFEKAVFWYLR
                     F"
     gene            406..1200
                     /locus_tag="ACF3T5_002758"
                     /note="aph(3')-IIIa"
     CDS             406..1200
                     /locus_tag="ACF3T5_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFD3633260.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1610..1693
                     /locus_tag="ACF3T5_002759"
                     /pseudo
     CDS             1610..1693
                     /locus_tag="ACF3T5_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MLS leader peptide"
BASE COUNT          560 a          278 c          411 g          513 t
ORIGIN      
        1 ctaccaagac gataaatgcg tcggaaaagt taaactgcga aaaaattgga accggtacgc
       61 ttatatagaa gatatcgccg tatgtaagga tttcaggggg caaggcatag gcagcgcgct
      121 tatcaatata tctatagaat gggcaaagca taaaaacttg catggactaa tgcttgaaac
      181 ccaggacaat aaccttatag cttgtaaatt ctatcataat tgtggtttca aaatcggctc
      241 cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta
      301 tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc
      361 ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga
      421 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
      481 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
      541 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
      601 tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg
      661 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
      721 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
      781 gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac
      841 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt
      901 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
      961 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
     1021 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
     1081 cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg
     1141 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
     1201 tacctagatt tagatgtcta aaaagcttta actacaagct ttttagacat ctaatctttt
     1261 ctgaagtaca tccgcaactg tccatactct gatgttttat atcttttcta aaagttcgct
     1321 agataggggt cccgagcgcc tacgaggaat ttgtatcgat aagaaataga tttaaaaatt
     1381 tcgctgttat tttgtacatt taacttgacg gtgacatctc tctattgtga gttattagtg
     1441 gtacagtttt caaccgtttt aattataaaa aagtggtgca tttttaaatt ggcacaaaca
     1501 ggtaacggtt attgcaggtg tatttcttat ctatgggttt aacatggatt ttatcattaa
     1561 aatcatgagt attgtccgag agtgattggt cttgcgtatg gttaacccta aagttatgga
     1621 aataagactt agaagcaaac ttaagagtgt gttgatagtg cattatctta aaattttgta
     1681 taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt
     1741 catgttggta ttccaaatgc gt
//
LOCUS       DAXLPI010000226         1752 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXLPI010000226 DAXLPI010000000
VERSION     DAXLPI010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.235"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            383..1633
                     /locus_tag="ACF3T5_002760"
     CDS             383..1633
                     /locus_tag="ACF3T5_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFD3633261.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     ETILEFNDSYHFDQIVIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFS
                     RMFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQH
                     FLENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGK
                     GRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQ
                     VIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQ
                     SQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARK
                     FTRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          577 a          335 c          310 g          530 t
ORIGIN      
        1 ttgtctaata gtttttattc aaaatgtttc tagattataa aatttattga ataacacatt
       61 cctaacggaa tttgtttcga tattgtgtga tttctcagag tacaatggat gtatggaaag
      121 gatccaacgg gaatttgcta tttcctcctg tagcgtttgt ctaccctgtt tagtctgtta
      181 cccgaaccac tgttatcact accaactcat tagctgtttc gataatggac tagactttca
      241 aatagtagtt tggaaggcag cttctagctc ttttatcacc gtattctatt aggcgaggtt
      301 gtggctatac agtgagtttc tgggaaatcc cacagtatcg aagcattttc caaaatttat
      361 atcgaaagga ggtccttctt ccatgaaatt atttgtcggt attgacgtta gctctgaaaa
      421 actagatgtt tgttttttaa cagacgacaa tcaattgtct atcttatctg aaatctctgt
      481 tgccaatgac atcgaaggtg cttcattcat acgagaaaca attcttgaat tcaatgacag
      541 ctaccacttt gatcaaattg tgattggcat ggagtcaacg tccatgtaca gcttccatcc
      601 ttccatgttt tttcatgaag atgagaaact aaagaagctc aacacacttg taacgattga
      661 aaatcctttt cgggtgaaac aatttagtcg catgtttgat gagaataaaa ctgatcgaaa
      721 cgatgcatta agaatcgctg atttcttgcg tattcaacga ttcacaactt cccctatcaa
      781 agaagagaaa tacatggcct tacagcgttt aacacgtact cgctatcaac ttattaagca
      841 attgattcgt acaaagcaac attttcttga aaatctaacg tacaaatgta atacgcttgc
      901 tcgagaaatg cgagacgaat cgacgagtct ttttagtgca accctcattt cactcatgac
      961 ggaagatttc acattggatg aacttgctga gcttccttta gaagctttct gtgatttact
     1021 tcaagaaaaa ggaaaaggcc gtttcaagca accagaaaaa atcgctaaag caattcaacg
     1081 tgcaattacc atgagttacc gcctaggtgc tcttgcacaa gaatcaatta atgttgtttt
     1141 aagtgttctc gtacgagaaa tacgagcact tgaaaaaaat atcaaagaat tagataaagc
     1201 catcgaacaa gtaattgttg tcattccaga atatcaatgc ttaaccagta ttccaggcgt
     1261 tggtaaagtc tatgctgccg gtttaatcgc tgaaattggt caaatcgaaa gatttgaaga
     1321 tcagacaaaa ttggcaaaat acgctggatt aagttggaaa gtgaaccaat ctggaaacta
     1381 tcagtcgcaa aatacacctc tgacaaaaca aggaaatcga tattttagat actacttagt
     1441 tgaagctgcc aactctgtaa aaaattatct tcctgaatac aaagcttttt atcaaagtaa
     1501 atataaggag gttccaaagc atcaacacaa acgtgcactc gtcctaaccg caagaaaatt
     1561 tacgcgtctg gtggatacgc tactacgtaa acaccaactc tatacgccac caaggagtgt
     1621 gatagagaaa taacaatccg ttattgacgc aaatccttga atcaacccga aaaaaatttg
     1681 attttgatcg ggtctagttt cgtgcgcttc aaaacatatt actcaataaa gaaaatctta
     1741 aatttcatct tg
//
LOCUS       DAXLPI010000227         1741 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXLPI010000227 DAXLPI010000000
VERSION     DAXLPI010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.236"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            242..1555
                     /locus_tag="ACF3T5_002761"
     CDS             242..1555
                     /locus_tag="ACF3T5_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HFD3633262.1"
                     /translation="MATLHENRLLFNSNVTVSHSGGNLSSDSGLILAKEFMNKFEFSQ
                     ILCKNIQIQDDRLYHVHENESILEQIILQLIAGYPTDSSANILATDPIFQAVLSKKRL
                     ASQSSISRFWDRISTENIVQLQKVNQILIDKARTIRNTNEMIFDLDSTHSDTFGNQEM
                     TDYNAHYQTTGYHPLVAFDGLTGDFLKAELRSGNVYTSKGVAAFTRPLFEHYHSVTPV
                     STIMVRADSGFAMPDLYELCEEYDSLYTIRLKSNRNLYRIADQFITIKDHHDWSKKEV
                     HYYNATYQAKSWGKSRRICIKSTREASELLFRHEFIITNFSKDVSPEMVFQTYSKRGT
                     MENYIKEAKNGFYLDKTDSPRFIENHARMIVSLLAYNIVNFMRTLCFTNETKGYQVST
                     IRLFLFKIAGKIVHSGRRQYLKLSSYHVYQKLFYRILQNIQVLQC"
BASE COUNT          580 a          325 c          295 g          541 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgacttga
       61 ctgcctagac ttcaatcaat attaattcct gaataattca tagtttttca aaaacgtttc
      121 aaaaaccttg ttataacagt ctattttgtt tcgtttattc gcaccctttg ggtgtacaaa
      181 aagaacccca atctgctatg gttaaagtga ctaaacctaa ccaaagaaag gggttctctc
      241 aatggcaact ttacacgaaa atcgtctact tttcaattca aatgttacag tatctcactc
      301 tggtggaaat ttgtcctcag attccggctt aatattagca aaagaattca tgaacaaatt
      361 tgagtttagc caaatcttat gtaaaaacat ccagattcaa gatgaccgac tgtatcatgt
      421 tcatgaaaat gaatctattc tggaacaaat catcttgcag ttgattgcag gctatcctac
      481 agattcttca gccaatatat tagcaacgga tcctatcttt caagctgttt taagcaaaaa
      541 gcggttggct tcacagtcat ccatttctcg cttttgggat cggatatcaa cagaaaatat
      601 cgtccagcta caaaaggtta accagatttt gattgataaa gcacggacga ttcgaaatac
      661 gaacgaaatg attttcgatt tagattctac tcacagtgat acctttggaa accaagaaat
      721 gaccgattat aatgcgcatt accagacaac cggttatcat ccactcgttg cctttgatgg
      781 tcttaccgga gactttctta aagcagaact tcgttctgga aatgtctaca cctcaaaagg
      841 cgtcgcagca tttactcgtc cattatttga gcattatcac tctgtgacac cagtaagcac
      901 tattatggta cgtgcagata gtggattcgc aatgcctgat ttgtatgagc tttgtgaaga
      961 gtacgatagt ttgtatacta tccgtttgaa atccaatcgt aatctgtaca gaatcgctga
     1021 tcaattcatt accattaagg atcatcacga ttggagtaag aaagaagttc attactataa
     1081 cgccacttat caagcaaagt catggggaaa atccagaagg atctgcataa aatcaaccag
     1141 agaagctagt gaattgcttt ttagacatga attcatcatt actaattttt caaaagacgt
     1201 ctctccagaa atggttttcc aaacgtattc aaaaagagga acgatggaaa attacattaa
     1261 ggaagcaaaa aacggctttt acttggataa aacagatagc ccacgtttta tagaaaatca
     1321 tgctcgtatg atcgtaagcc tattggctta caatatcgtg aacttcatgc ggacactgtg
     1381 ttttaccaat gaaacgaaag ggtatcaggt ttctactatt cgcttatttc tctttaaaat
     1441 tgctggaaag atagttcatt ccggaagaag acaatacttg aaattaagta gttaccatgt
     1501 ttaccagaag ctattttatc ggattctaca aaacattcag gtactgcagt gttaattgcg
     1561 tttattgcgc cattcaaaaa aattcgttaa ccatccgata ggatgagtgc gcccaaaatg
     1621 aggtctttct tgaacttaat cagttagcaa aaataaatta aaccctttag aaaaatattt
     1681 ttgagaaaaa gaaccattga gcataaaact aaacagataa ttataaaatt tggtagttta
     1741 t
//
LOCUS       DAXLPI010000228         1710 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXLPI010000228 DAXLPI010000000
VERSION     DAXLPI010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.237"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..305)
                     /locus_tag="ACF3T5_002762"
     CDS             complement(<1..305)
                     /locus_tag="ACF3T5_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFD3633263.1"
                     /translation="MSKNNNKNNSSELENCLFNPKKLEDSINKVKRINDLPLVLTNKD
                     LKEQFQISDSTLNRLIKLDNFPRCWFGIKGHYLRDDILEWIKKGKEEYFCDQFRLLR"
     gene            complement(657..902)
                     /locus_tag="ACF3T5_002763"
     CDS             complement(657..902)
                     /locus_tag="ACF3T5_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633264.1"
                     /translation="MVDKPKKWQYPLVPLSIILSALEEDPISIKMILKHYDNYIAKLC
                     LTNGFNEVGQFITYVDEYMLRQLEVKLIEAILKFKVN"
     gene            complement(896..1297)
                     /locus_tag="ACF3T5_002764"
     CDS             complement(896..1297)
                     /locus_tag="ACF3T5_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFD3633265.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNEAKSIRKRNTRIKDNEEPLN
                     DVNEGKYPQTNFDEQDVYFLFGMEILISDQKLSAAIDQLSDIRRKIILLYYFADFNDA
                     EIGRILNMSTSGIWYQRTKAVEQLKMGYDLW"
     gene            complement(1534..>1710)
                     /locus_tag="ACF3T5_002765"
     CDS             complement(1534..>1710)
                     /locus_tag="ACF3T5_002765"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFD3633266.1"
                     /translation="TTQVGKQAKKAKMLPKTGEEDTNMITIFGGLLLLGSLGLIDYKK
                     RESKKSSSRIERSK"
BASE COUNT          529 a          306 c          219 g          656 t
ORIGIN      
        1 tctcgtaata aacgaaactg atcacaaaag tattcttcct ttcctttttt tatccactcc
       61 aaaatatcat ctctaaggta atgccctttg atgccaaacc agcatcttgg aaaattatct
      121 agttttataa gacgatttaa agtactatca cttatttgaa attgttcttt tagatcttta
      181 tttgttaaaa ctaacggtag gtcatttatt ctctttactt tattgataga atcctctagc
      241 tttttgggat taaataaaca attttctaac tctgatgaat tatttttatt gttattcttt
      301 gacatgatta tctcttccct tctattatta aggattaatc tatcagctgc ctttgtcaaa
      361 agcacatagg aatttcacct ctccccaagt ttacggcgag ccttacggaa gtatcattat
      421 cccaacctca aatcatcgct ttttattgac tataaaaatt attttagaat atttatcgct
      481 ccttctttat aataagagat catggcgtaa ctaaaatatg ctccttgaag tgttaacgta
      541 ctgatagaat atatttattt ttcaatgaac aataggtttt ctccctaaaa gaatccgcta
      601 atctaaagta ttagaagact ctttttggta gaaaaagtag aaaggggagt ttttagctag
      661 ttaactttaa atttcaatat agcttctatt aatttaactt ctaattgcct tagcatatat
      721 tcatccacat aggtaataaa ttgccccacc tcattaaatc catttgttaa acataactta
      781 gctatataat tatcataatg tttgagtatc atcttgatcg aaattggatc ttcttctaga
      841 gcagatagga taatagatag tggaactaat ggatactgcc attttttggg tttatctacc
      901 ataggtcata ccccattttc aattgttcaa ctgccttcgt tcgttgatac cagataccac
      961 ttgtactcat attgagaatt ctaccgattt cagcatcatt aaaatcggca aaataataaa
     1021 gtagaataat cttccttctt atatccgata actgatcaat tgctgctgat aacttctgat
     1081 ctgaaattaa tatctccatt ccaaataaga aatagacatc ttgttcatca aaattagttt
     1141 gtggatactt cccttcattt acatcattca gtggttcctc attatctttt attctagtgt
     1201 ttcgttttcg aatagactta gcttcgtttc gtaatacttt tttgcagtag gcatcaaatt
     1261 ggtgttgtat agatttctca ttatagtttt gttgcacgat caatatgctc ctttatcccc
     1321 tttccactaa tacaacaatc agagagtgcc tttttttcga aaaaactaaa aaaaaacaaa
     1381 aaataatcat actttttagg tggaatttca ttctcaaaaa aaaaaaaaac gattagtatc
     1441 actaatcgtt tttgagtatt ccttgcaaaa gccaaattac ttttatagtt aaaatttgat
     1501 attgataatc atccttcttt cggtagtggg taatcatttt gatctttcta ttctacttga
     1561 tgatttttta gactctctct ttttataatc aattaggcct aaacttccca acaatagaag
     1621 accaccaaag atagtaatca tatttgtatc ctcttcacct gttttgggta acattttcgc
     1681 ttttttagct tgtttcccta cttgtgtagt
//
LOCUS       DAXLPI010000229         1684 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXLPI010000229 DAXLPI010000000
VERSION     DAXLPI010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.238"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            132..506
                     /locus_tag="ACF3T5_002766"
     CDS             132..506
                     /locus_tag="ACF3T5_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFD3633267.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="ACF3T5_002767"
     CDS             complement(620..1249)
                     /locus_tag="ACF3T5_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFD3633268.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="ACF3T5_002768"
     tRNA            complement(1383..1455)
                     /locus_tag="ACF3T5_002768"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
BASE COUNT          588 a          245 c          288 g          563 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc cggcatagct
     1681 cagt
//
LOCUS       DAXLPI010000230         1581 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXLPI010000230 DAXLPI010000000
VERSION     DAXLPI010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.239"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            50..1528
                     /locus_tag="ACF3T5_002769"
     CDS             50..1528
                     /locus_tag="ACF3T5_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFD3633269.1"
                     /translation="MKKLSQKRLAQIIIDSRKSLKMTQQQLAVATGINRALISRLESL
                     DFIPSIQQLESLAEVLNFEPTDLFINESNKSNRTKHQSKNIAVAGTGYVGLSIATLLA
                     QHNQVTAVDILPEKVKQINNKKSPIQDDYIEKYLAEKNLNLIATTDGAAAYREADFVV
                     IAAPTNYDSKQNYFDTSAVESVIKLVLEVNPKAIMVIKSTVPVGYTESIRKKYNTKNI
                     LFSPEFLRESKALYDNLYPSRIIVGTDQTDKTLREASHTFADLLQEGAIKKDIETLFM
                     GFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQEIIHGVCLDPRIGTHYNNPS
                     FGYGGYCLPKDTKQLLTNYNDVPQNMISAIVDSNRTRKDFIADRVLELAGTYEANSSW
                     NAEKEKEVVIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGATIVIYEPTLENNTTFFG
                     SKVINNLDDFKNISHSIIANRYDSNLDDVKEKVYTRDLFQRD"
BASE COUNT          593 a          270 c          264 g          454 t
ORIGIN      
        1 agaagaaaaa attgtttgtt attattagca acaaaaaaag gagtgcatca tgaaaaagtt
       61 atctcaaaaa cgtttagctc aaatcattat tgattctaga aaatctctga aaatgacaca
      121 acaacaactt gcagtagcta ctggtatcaa ccgtgcctta attagtcgat tagaatctct
      181 cgattttatc ccgtcaatac aacaattaga atcattagca gaagttctaa attttgaacc
      241 aacagatcta tttataaatg aatcaaataa atcaaacaga acaaaacatc agtcgaagaa
      301 tattgctgta gccggtactg gttacgtagg tctttccatt gctactcttc ttgcccagca
      361 caatcaggta acagcagtag acattcttcc tgaaaaagta aaacaaatta acaataagaa
      421 atctccaata caagacgatt acattgaaaa atatctagct gaaaaaaatc ttaatttaat
      481 tgcaacgact gatggtgcag ccgcctatag agaagccgat tttgttgtca ttgctgctcc
      541 aacaaattat gatagtaaac aaaattattt tgatacttct gctgtagagt cagtaatcaa
      601 attggtgtta gaagtcaatc caaaggcaat aatggtcatc aagtcgacgg tacccgtagg
      661 ctataccgaa agtattcgta aaaaatacaa tactaaaaat attctattca gcccagaatt
      721 tctcagagaa tctaaggcat tatatgacaa tctatatcct tcgcgaatta ttgtcggcac
      781 ggaccaaact gacaaaactt tacgagaagc ctctcacact tttgcagatt tactccaaga
      841 aggagcaatc aaaaaagata tcgaaactct tttcatggga ttcacagaag ctgaagcagt
      901 taaattattc gctaatactt accttgcact tcgagtatct tacttcaatg agctggatac
      961 ttatgcagaa atgaaaggac taaatacaca agaaatcatt cacggggttt gcttagatcc
     1021 aagaattggc acacattata ataatccatc ttttggatat ggaggctatt gtttaccgaa
     1081 agatacaaaa caacttctca ctaactataa cgatgttcct cagaatatga taagtgcaat
     1141 tgtcgatagt aatcgtacaa gaaaagactt tatagcagat cgagtgttag aacttgctgg
     1201 cacgtatgag gctaacagtt cgtggaatgc agaaaaagaa aaagaggtag tcataggtgt
     1261 ataccgtctt acaatgaaga gtaattcaga caacttccgt cagtcatcta ttcaaggagt
     1321 tatgaagcga attaaagcaa agggggcaac tattgtaatt tatgaaccaa cacttgaaaa
     1381 taacactaca ttttttggtt caaaagtaat aaataattta gatgatttta aaaatatcag
     1441 tcacagtatt attgctaatc gttacgatag taatcttgat gatgtaaaag aaaaagtata
     1501 taccagagat ttatttcaac gagattaaaa aataacacct cagatttgta gtgtctgagg
     1561 tgtttctata aacttttata a
//
LOCUS       DAXLPI010000231         1572 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXLPI010000231 DAXLPI010000000
VERSION     DAXLPI010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.240"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            84..1130
                     /locus_tag="ACF3T5_002770"
     CDS             84..1130
                     /locus_tag="ACF3T5_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD3633270.1"
                     /translation="MSVKISVIVPCYNVEKYIDECINSLINQTLKDIEIILLNDGSTD
                     NTLAILKKYEKLDSRIKVYEHKNMGLGPTRNRGIKIANGEFLAFVDSDDFVSNTMFES
                     LYNCAIAENADIAIGEVGIFSDREIERVKIRKSLTNLKTIELATYNKKEFFKQFYFNR
                     LYSHNAWDKIYRRSLVLEFNLSFGDNKIIFAEDNWFQLQVFMTNTRICFVDKLCYYYR
                     QQNNSIMHKPKSNLVFRHGNMIADYEKLIRQNGNNIIDKKACMLVAFDVLIMEIFNQK
                     NVGGTCEDYKNALVSLKHHKILRENISNIVRMKAYELEPNMRKKLALVTISIMFNFKM
                     YKTAYNMLWRIYNR"
     gene            1144..>1572
                     /locus_tag="ACF3T5_002771"
     CDS             1144..>1572
                     /locus_tag="ACF3T5_002771"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFD3633271.1"
                     /translation="MRKLTFVIGIMGKGGAERVIANLANNFIQDNWEIDIITIYGNRQ
                     DYELNRKIQVHPIICKSSFRAFRPLERFKELRKKIREIQPDLVISFLADVNIHVIISL
                     LGVKQKLIVSERNDPQRNPEEPWIRKIRDFLYKNVDGIVFQ"
BASE COUNT          618 a          146 c          295 g          513 t
ORIGIN      
        1 ggaattagtg ctaagtttgc attgaaaaac aaatgggtat ttttaattta tatttataac
       61 aaattgataa agaggtaata ataatgtcag ttaagattag tgtaattgta ccttgttata
      121 atgttgaaaa atatattgat gagtgtataa atagtttgat aaatcaaaca ttgaaagata
      181 ttgagattat tttgttaaac gatggttcaa cagataatac gttggcgata ttaaagaaat
      241 atgaaaagct tgattctaga ataaaagttt acgaacataa aaatatgggg ttagggccaa
      301 ctagaaatcg agggattaaa atagctaatg gagaattttt ggcttttgtg gattctgatg
      361 attttgtttc caatactatg tttgaaagtt tatataattg tgcaattgca gagaatgcgg
      421 acattgcaat tggagaagta ggcatttttt ctgatagaga aatagaaaga gtaaaaataa
      481 gaaaaagtct aacgaactta aaaacaattg aattagcaac atataataag aaagaatttt
      541 ttaagcaatt ctatttcaat cgtttatatt cacataatgc ttgggataag atatatagac
      601 gtagtttagt attagagttt aatttatcat ttggtgataa caaaataatt tttgcagaag
      661 ataattggtt tcaattacaa gtatttatga caaatacaag aatctgtttc gtagataaat
      721 tgtgttatta ttatcgccaa caaaataatt ctataatgca taagccaaaa agtaatttgg
      781 tttttagaca tggaaatatg attgctgatt atgaaaagtt gataaggcaa aatggaaaca
      841 acataataga taaaaaagct tgtatgttgg ttgcatttga tgtactaatt atggagattt
      901 ttaatcaaaa aaacgttggt ggaacatgtg aagattataa gaatgcatta gtttctttga
      961 aacatcataa aatattgaga gaaaatattt caaatatagt tagaatgaaa gcatatgaac
     1021 ttgaacctaa tatgagaaag aagttagccc ttgtgaccat tagtataatg tttaatttta
     1081 aaatgtataa aacagcgtat aatatgctct ggagaatata taatcgataa agaggtagga
     1141 aatatgagga aattgacctt tgtgatagga attatgggaa aaggaggggc agaacgagta
     1201 attgcgaatt tggcaaataa ttttattcaa gataactggg aaattgatat tataactata
     1261 tatgggaatc ggcaagacta tgaactgaat agaaaaatac aggtacatcc aataatatgt
     1321 aaaagttcgt ttcgtgcatt tagacctttg gaacgcttta aggaacttag gaaaaaaata
     1381 agggaaatac agcctgatct ggttatttcg ttccttgctg atgtaaatat ccatgtcatt
     1441 atttcattgt taggagtaaa acaaaaatta attgtttctg aacgaaatga tccacaacgg
     1501 aatccagaag aaccatggat taggaaaatt agagattttc tttataaaaa tgtagatgga
     1561 attgtttttc aa
//
LOCUS       DAXLPI010000232         1569 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXLPI010000232 DAXLPI010000000
VERSION     DAXLPI010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.241"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..261
                     /locus_tag="ACF3T5_002772"
     CDS             <1..261
                     /locus_tag="ACF3T5_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633272.1"
                     /translation="DLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDFI
                     LGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            440..997
                     /locus_tag="ACF3T5_002773"
     CDS             440..997
                     /locus_tag="ACF3T5_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633273.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
BASE COUNT          581 a          204 c          328 g          456 t
ORIGIN      
        1 gatctaggaa tctattttag tcgaggaagt gcgcaatatt atatggggga aaatgtacaa
       61 tcaatgtcat taaaaccaca tatagccctt agaaatctcg aggaaggttt taaaagagag
      121 gtagacttta tattaggaga aagtggatac ataagaactg tacttaattt cggagaagcg
      181 caagaagttt tgacacaata cggaattacc ttaatcaact gggaaatatc tgaaccgatt
      241 gttaacaatt ttggtgatta gaaaacaatt acgataaata gaaacaagcc aaatcatcag
      301 caagtaaaag ctgatgattt ggcctttctt atacgagtag taggaattca tagcgaatat
      361 tattacgacc agtagatatt ttgatattaa aggagtatta cgaactaata tgtgagaatg
      421 tagaggtgat gtgcgaataa tgagaaattt atcaaataga aacaaaatat taatcattat
      481 tgttgttata gctgtgtttc atctgggaac gaatgcggtt ttaagtagaa taatattggg
      541 tcctaagcca ccaagaccag aaattacccg aggggagttt gattttagat tagaatatga
      601 agtagatggg gaaaggattg tgatagaaga tacaattgtt gcattatttg atggattttc
      661 tgcagatgca ggtagcatgg catggtatcg cacgtggcgt ctacatttag caagtgatag
      721 acgatcacga aatatcttat tagatgaatt agaggatggt cgaagaatat attatgtccc
      781 agaaagtgct aactattcta tgggagatgt acagaaaaaa agagagccta atcctaattg
      841 gtatccattt aatggcgtaa caatagaata tccacgcaat aaaacacctg aaataggtgc
      901 aaaatttata agtggtttag aagatctgta cgataggttt ggaatccgtc ttattagttg
      961 ggaacacgat ccaccaatag aaaatcgctt tgaataatcg aaatctacta atccatttca
     1021 tcagccagtg aatgttgtaa tggaacaatt tggtttagtg ttcaaaacca ttgaaatact
     1081 aggctgtgat cagcaaaaat gaatctgaat aaaaaagaga gcaactatta gctgagagga
     1141 gttaatagtt gttagcttta tacgaatcaa agtggcaaat acagatgcgt atgcatttgt
     1201 gtttgccatc ttgcgtttgg tagacgagat aggtataagc taaagaaaca acttgtttaa
     1261 aataaacgac ctagctcaac aaatgaccat ctaatcaact aacagcatac ataaaagcaa
     1321 ctatttttga agtagaattg aaatcgggat aacttaaaaa gtgattggga tactagtgag
     1381 aatcaccgta cataaatatt actcgttaag ggaaattgag aaattttgta tgaaaattta
     1441 agaattagtt agtatgcgca agtcaaaaat atgaataaat tatttggtgg agataatttg
     1501 cttagcttgt cataagaaat gagataatct ggcagatgca agcttttgga caccatttat
     1561 tcggaataa
//
LOCUS       DAXLPI010000233         1553 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXLPI010000233 DAXLPI010000000
VERSION     DAXLPI010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.242"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            40..1506
                     /locus_tag="ACF3T5_002774"
     CDS             40..1506
                     /locus_tag="ACF3T5_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFD3633274.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          429 a          290 c          327 g          507 t
ORIGIN      
        1 taatattttc tgaaaattta aagaattggg gtatagatta tggaaaaaca gcaagacaag
       61 ggcttcttcg ggcagccgaa gggattgtca actttgttct tcactgagat gtgggaacga
      121 tttagttatt acggtatgcg tgcattactt atctattata tgtacgatac agttgccaac
      181 gggggacttg gattaccacg agcaacagca ttagctatca tgtcgatcta cggatctttg
      241 atctacatgt ctagtatcgt cggcggatgg gtatctgacc gagtgttagg agcaaaacgc
      301 actgtattct tcggtggagt tttcattatg cttggccaca tcgtcttggc tttaccattt
      361 ggtgtaagta cattgtttgt ttcgatggca ttgattatta tcggaaccgc ctttttgaaa
      421 ccaaatgttt ctggcatggt cggacatctt tattcaaaga ctgacttgcg aagagatgca
      481 ggattttcga ttttttacat gggtgtcaac ttaggggcat tattagcacc actaatcgtt
      541 ggtacaatcg gtcaagaata taactatcat ttagggttct ctttagctgc tattgggatg
      601 ttctttggat tattacaata catcatccaa ggacgcaaga cattagacgg agtgggtaca
      661 acaccgccga atcctttgac aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc
      721 gcacttgttg ttatcgctgc agtattcggc ggggcacaac taactggtca tctgacaatt
      781 gacttttttg tcaatacaat cagtgtattg gggattatct taccgttgta ttactttatc
      841 aaaatgctga cttcaaaaga tgtaactgct gaagaaaaac caagagtatg ggcttatatc
      901 ccattattcc ttgcagcaat cattttctgg tcattacaag aacaaggttc atctatctta
      961 gctttatttg ctagtgaacg tacacaatct acgctatttg gtgtaacgat ccaagcaagc
     1021 tggtatcaat cattgaatcc aattttcatc tttattctga caccagtatt cgtaacgatg
     1081 tggacgaaac ttggtgaacg tcaaccatct acagtagtga aattctctct tggactttta
     1141 tttgcaggat tatcgtactt gctattgatg ttgccgggta tgctttatgg tacaagtgga
     1201 aaagtaagtc cattgtggtt gttgggaagt ttcttcatcg tggttattgc tgaattgtgt
     1261 ctttcaccag ttggtttgtc tgtgacaaca aaattagcac cacgagcatt tgaatcacaa
     1321 acaatcgcta tttggttctt agctgatgct tcttcacaag caatcaatgc acaaattgca
     1381 cgcttctaca caccgggaac agaatctgct tacttcggta ttatcggtgc agtagcaatc
     1441 gtcggtggta tcttgctatt tactgtgaaa gaaccaatca aaaaactaat gggctctatt
     1501 cattaatcaa tgaacataaa aacaggaatt gcttatttgc agcagttcct gtt
//
LOCUS       DAXLPI010000234         1526 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.243, whole genome shotgun sequence.
ACCESSION   DAXLPI010000234 DAXLPI010000000
VERSION     DAXLPI010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.243"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            26..991
                     /locus_tag="ACF3T5_002775"
     CDS             26..991
                     /locus_tag="ACF3T5_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFD3633275.1"
                     /translation="MAVVLTVAANKGGVGKTLITINLAGAIRRSFPEAKILVVDTDAQ
                     GNSTKSFRVKVAKDQNTIYDVFMDNATVEETIVQTYDDHIDVLPANSDNNYLEFDKME
                     QFRDTILEWFVSLLKKFKDNLTEIMTVSGLKKKMNNIIDPSANYFNALDGKFDNVSDK
                     YDYIIFDTPPELKQVTSSVLSIADVVIVPYEPDLNGVDGVTHLISRVNTLKEKYNPNL
                     RIGGVLANKVYNTNLHAKMINAMMKYTNRNNYHYFDSELPRSITFADKLVRNGMPITM
                     SSPENKFAQHFYKLIIEMNKLGLLSKNGKELEIPHKLYVESDGEE"
     gene            991..1455
                     /locus_tag="ACF3T5_002776"
     CDS             991..1455
                     /locus_tag="ACF3T5_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633276.1"
                     /translation="MPRINRKKPTGDFESVFDEELEELETPNDTIDSIPETEVTVSNS
                     FPSDKETITENFNEANRFGLSKTSKSTSSELVARTFKYKKETIQRVESLVYKDQRHSK
                     KIPGTKGFISDFMDNAIWQHLLQLGLATEEEVQKHLNSYSKYPLNFDNYEKK"
BASE COUNT          567 a          234 c          264 g          461 t
ORIGIN      
        1 atattattta gggaggaatt gaaaaatggc agtagttttg actgttgcag caaacaaggg
       61 tggtgttggt aaaactctta ttacaatcaa tctcgcagga gctatccgta ggagttttcc
      121 tgaagcaaaa attcttgtcg ttgatactga cgctcaagga aatagtacaa agtcttttcg
      181 tgtgaaagta gctaaggatc aaaatacgat ttatgatgtc ttcatggata atgcaacagt
      241 tgaagaaact attgttcaaa catatgacga tcatatagac gttcttcctg cgaattctga
      301 taacaactat ttagagtttg ataaaatgga acaatttcgt gataccatat tagaatggtt
      361 tgtatcttta cttaaaaaat ttaaggataa cttaactgaa atcatgactg tttcaggatt
      421 aaagaaaaaa atgaataata ttattgatcc cagcgcgaac tattttaatg ctcttgatgg
      481 taaatttgat aatgtgagtg ataaatacga ttacatcatt ttcgatacac caccagagtt
      541 aaaacaagtt acctcatctg ttctttcaat tgcagacgta gttatcgtcc cttatgaacc
      601 tgatttaaat ggggttgatg gagttacaca cttaatatct cgtgtgaaca cattgaaaga
      661 aaaatacaat ccaaatttac gcattggagg cgtacttgcc aacaaagtct ataatacaaa
      721 tctacatgca aaaatgatta acgcaatgat gaaatatact aatagaaaca attatcatta
      781 ttttgattct gaactcccaa gatcaattac ctttgctgat aaattagttc gaaacggaat
      841 gccgataaca atgagttcac ctgaaaacaa atttgctcag catttttata agctgattat
      901 cgaaatgaac aagttaggtt tactttctaa aaatggtaaa gaactagaaa ttcctcataa
      961 gttatatgtt gaaagtgacg gtgaagaata atgccaagaa taaacagaaa aaaaccaaca
     1021 ggagattttg agtctgtatt cgatgaggag ctggaagaac tagaaacacc taatgacact
     1081 atcgatagca tacctgagac tgaggtaact gttagcaata gttttccctc agataaagaa
     1141 acaattaccg aaaactttaa tgaagcaaac agatttggct taagcaaaac ttcgaaatct
     1201 acaagttctg aactagttgc acgtacattt aaatataaaa aagaaactat acagcgagtt
     1261 gaaagtttag tgtacaaaga ccaaagacat tctaaaaaaa ttcctgggac taagggattt
     1321 atttcagatt ttatggacaa tgcaatttgg caacatttat tacagctggg acttgccact
     1381 gaagaagaag ttcaaaaaca tctaaacagc tatagtaagt atccacttaa cttcgataat
     1441 tatgaaaaga agtagcttat cgctatttct ttttttatag ggcgaaattt ttatatcact
     1501 atttgtatta ccacaatatt acgata
//
LOCUS       DAXLPI010000235         1521 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.244, whole genome shotgun sequence.
ACCESSION   DAXLPI010000235 DAXLPI010000000
VERSION     DAXLPI010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.244"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..381
                     /locus_tag="ACF3T5_002777"
     CDS             <1..381
                     /locus_tag="ACF3T5_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633277.1"
                     /translation="GISVTGTTIPTFADSTNEPTTVVDNNQELTPLDRDGIAIGNPTN
                     DLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSRNWKEKRNGYWYYGK
                     LYLQSYKSAGNNWIATYSGTLTRK"
     gene            391..594
                     /locus_tag="ACF3T5_002778"
     CDS             391..594
                     /locus_tag="ACF3T5_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633278.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            805..1389
                     /locus_tag="ACF3T5_002779"
     CDS             805..1389
                     /locus_tag="ACF3T5_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3633279.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          537 a          221 c          284 g          479 t
ORIGIN      
        1 ggaattagtg taactggaac tacaattcct acatttgctg atagtacaaa tgaaccaact
       61 actgtagtag ataataacca agaattaact cctttagata gggacggaat agccattggt
      121 aacccaacta acgacttaac tgttactgaa aattatttaa tagtttctcc attacttgct
      181 ctagcagctt ctaaaacgat acgagtgaca ggaacgatga tttttgataa agttggttgg
      241 ccttctagac ctcctcaatc acggaattgg aaagaaaaaa gaaatggtta ttggtactat
      301 ggaaaattgt atttacaaag ttataaatca gctggaaaca attggattgc gacttattca
      361 ggaaccttaa ctagaaagta gggatttttt atgccagaat ttgtgaacac accaaatttt
      421 tatcaagata gcgatattct aagtaaaatt aatttaccga aagaagaatc agctttacaa
      481 gttcagcgaa ctttacagga gacacaaaaa attcaaaaat caatttctaa tctagaagat
      541 tctgaagaaa aaagaaaaaa aagagaccaa aaaacatatc aaaaagaaaa ataatgattt
      601 taaaaaaaga gtgtctagtc gacattcttt ttttctaatt gaaagtgttc ataaaatcac
      661 ccatttctgg acactttctt tttttgcttt atttttcaaa aaaacaattc atttcatgtt
      721 cataaaccca taacaatatc tatgttcagt taaactatat ggataaagtt atgttacaat
      781 aaaattaatg aaggagtgat ttttttgaaa ataggatacg cccgggtatc tactggttta
      841 caaaatttgg atttacaaga agacagccta aataaatttg gttgtgaaaa aatttttact
      901 gatcatatga gtggggcaaa aagtaatcga ccaggattag aaatggccat tgattttgtt
      961 cgctctggcg atacacttgt cgtttggcgc ttagatcgtt taggtagaaa tatggaagat
     1021 ttgatttcaa tcgtcaatcg actgaatgag cgtggtgtga gttttcatag tattcaagaa
     1081 aatattacga tggacaaatc aagttctaca ggacagctca tgtttcattt gtttgcggca
     1141 tttgcagaat ttgagcgtaa tttgatcctg gaacgatccg cagcaggacg agcagctgct
     1201 cgtgcaagag gacggtttgg tggtcgtcct gaaaaattgt ctgaacaaga tttggaatta
     1261 ctgaagactt tagtggatag cggtacacct attaagacta ttgcggaacg gtggaacgtc
     1321 tcaagaacca ccatttatcg ttatttggca aaaatggagg agaaaaatgg aaagacaaat
     1381 tctcattagt ttttgtcatt taatgctgaa aatttttgcc atttatctag aaaagtgaca
     1441 atagtatgag aattttttgt aagtgtctac tgataactta ttctcaaatg ttgtaaggat
     1501 tttattcatt tgttaatatt a
//
LOCUS       DAXLPI010000236         1517 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.245, whole genome shotgun sequence.
ACCESSION   DAXLPI010000236 DAXLPI010000000
VERSION     DAXLPI010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.245"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..790)
                     /gene="mobQ"
                     /locus_tag="ACF3T5_002780"
     CDS             complement(<1..790)
                     /gene="mobQ"
                     /locus_tag="ACF3T5_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFD3633280.1"
                     /translation="MAIFHLSMTIAKREGGKRSLIAMASYRSGEKLYSELYEKTNLYN
                     HRTVKPEAFILKPDYVPNEFLDRQTLWNKMELAEKQVNSQLCREVNVALPIELNNTDQ
                     RSLIEEFVKDNFVSEGMIADVAIHRDDENNPHAHIMLTMREVDSEGNILNKRKKIPKL
                     DENGNPILLENGKIKMVSIKTNDWDRKSLVSEIRKDWADKVNQYLKDRNIDQQITEKS
                     HAELGKKELPTIHEGFYSKKLEEKGVISELKRKNLEIQSYNDILA"
     gene            1082..1381
                     /locus_tag="ACF3T5_002781"
     CDS             1082..1381
                     /locus_tag="ACF3T5_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633281.1"
                     /translation="MENQDQNINAINGNFNSKTKPKKRKKKTVSDIELQIKELQKKKE
                     ELILKSKADIGDFVLSTLSKLDISIESIEENKELFYDELETLLNANSQNFSELLK"
BASE COUNT          528 a          226 c          197 g          566 t
ORIGIN      
        1 cggctagaat atcattgtaa ctttgaattt ctaaattttt tctttttaac tcacttataa
       61 ctcctttttc ttctaatttt tttgagtaaa aaccttcatg aattgttggt agttcttttt
      121 ttccaagttc cgcatgcgat ttttctgtaa tttgttgatc gatatttcta tcttttaaat
      181 attggttgac tttatcagcc caatctttac gaatttcaga aacaagagat tttctatccc
      241 aatcatttgt tttaatagaa accattttaa ttttaccatt ttctaataaa atcgggtttc
      301 cattttcatc tagtttaggt atcttttttc gcttgttaag aatatttcct tcactatcta
      361 cttctctcat tgttagcata atgtgagcat ggggattgtt ttcatcatct ctatggatag
      421 ctacatctgc aatcattcct tcgctgacaa aattatcttt aacaaattct tctatcagtg
      481 atcgttgatc tgtattattt aattcaattg gcaatgctac atttacttct cggcaaagtt
      541 gagaattcac ctgtttttct gctaattcca ttttgttcca taaagtctgt ctatctaaaa
      601 attcattagg tacataatca ggctttaaaa taaaagcttc tggtttaaca gttctatggt
      661 tgtatagatt agttttttca tatagttcac tatacaattt ttcaccgctt cgataagaag
      721 ccattgcaat taaacttctt tttccgcctt ctctttttgc tattgtcata gataaatgga
      781 agattgccaa ttgtatcacc tctctttctt ttcaaacatg agcgtagcga attttaacaa
      841 acacgcttga cgataaaacg aaatgtaaat taagttttat agcacaatta ccactaaatg
      901 aaatttaggg gtataagtgc gcccttagta aatcatctaa gggaatcctt gataaattaa
      961 ttttaccact tatcattata aaagtaaata tgtttaatct gacattttca tttatattta
     1021 ttgattttag aaaaataata taatatgata gagttacata atatattagg aggcgtattt
     1081 catggaaaat caagatcaaa atattaatgc aattaatggt aattttaatt ctaaaactaa
     1141 gcccaaaaaa agaaagaaga aaactgtttc tgatattgaa cttcaaatta aagaattaca
     1201 aaagaaaaaa gaagaattaa ttctaaaatc aaaagcagat attggtgatt ttgtcctttc
     1261 tactctttca aaacttgata tttctattga atcgattgaa gaaaacaaag aattattcta
     1321 tgacgaatta gaaactttat taaatgcgaa tagtcaaaat ttttctgaat tacttaaata
     1381 gttatggcaa ataatgattt aaatgacatc aacaaaagaa tagaaaaact aaaaattcaa
     1441 aaaaatattt tgcgagcgaa tagcgagcaa aatattaaca gaaaaagaag aacaaaacgt
     1501 ctaattgaaa aaggtgc
//
LOCUS       DAXLPI010000237         1516 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.246, whole genome shotgun sequence.
ACCESSION   DAXLPI010000237 DAXLPI010000000
VERSION     DAXLPI010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.246"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            786..1172
                     /locus_tag="ACF3T5_002782"
     CDS             786..1172
                     /locus_tag="ACF3T5_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFD3633282.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1266..1457
                     /locus_tag="ACF3T5_002783"
     CDS             1266..1457
                     /locus_tag="ACF3T5_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633283.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
BASE COUNT          565 a          233 c          211 g          507 t
ORIGIN      
        1 tttataatac tgaattcgga attaaaaacc acgataggta actttaaaca aacatgaaga
       61 gactttaata agacttcttt atctgaaaac acttttgttt ctctctttcc tttctacatc
      121 attaaatagt attttatacg ttatctaaaa tactattcct ctgtcaaaat aagctaactt
      181 acttcattat aaaatatcca cttatcgctt tcatgtatat tccaccgtaa aaagtacatt
      241 ttatcaccga gaactcatca tttttcatca tgatgaatac tatttacata tatctagttc
      301 aaagacaata attattatga tccgttatta tctaagcact atacagataa taggaatttt
      361 tcgtttctaa atattccaat ttgggaatcc aataaaaatt ttcgttcaaa tacgtgtcta
      421 ttcgaactct tttctagttg ttaattatga agggggaaga aataaaaaat acaatctata
      481 cacgaataag aatattatcc aaaaggaggt tttagaaaag atttattata cgtaaaaagc
      541 tcaagacatt ttcttagcca taaggacatg tttgatattt aagaagatat atgaattttc
      601 ttttctaagt ataaagataa ctatccatta tcaactaaaa aaacattttt gaaaataaac
      661 atcagcagtc ctatcaatgc atgtgagctt atagaagtta ttcgcagcgc agcataattg
      721 cattagcttc ttttcgtgtt ttttgcctct tttcccttta aatcttaatg gaggaggtgt
      781 gcaatatgtc agataacgac gaacgcgtaa atgcccagtt gattcgattc tttgaaaagg
      841 tcattttgaa caccgctaat acttattata aaaaacaaga agaaattcaa aaacatgaac
      901 aacttgatca actcccacca tatttgttta ctaaagatac tataaaattt aaacaaccga
      961 ttaatgtttt aaatgtgact ctccttgttg aagatataaa attagcaaaa atccttcctt
     1021 tgttgaaaga acctgaacaa atttttctta tcgaaaaatt tatctttgat aaaactgata
     1081 aagaaattgg aaaggttctg gggattacaa gacagggtgc gacaaattta aaacatcgac
     1141 tttacaaaaa aattcaaaaa atgctggttt aatatctgca taataactaa ttttgtatgc
     1201 ttctttgctc gttctcctcc ttcccaaaga aatagattat aagtccatgg gaagaaggta
     1261 gatttatgaa ttctttaaaa gaagatttca ttttagcaaa ggctggaaat gaagaagctg
     1321 ttgaggcaat tctcaaaaga tttagctctt tgattcataa gcaatcctgg agaactggaa
     1381 aatatgatca agattgttat caagaatgta tgcttgcaat atatctagct atctcgaaat
     1441 tcgaaattaa agaataactc aactaaggga gtctgtgaca taagtaaaaa gatagtttca
     1501 aatttcgaac aataaa
//
LOCUS       DAXLPI010000238         1511 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.247, whole genome shotgun sequence.
ACCESSION   DAXLPI010000238 DAXLPI010000000
VERSION     DAXLPI010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.247"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            1..564
                     /locus_tag="ACF3T5_002784"
                     /pseudo
     CDS             1..564
                     /locus_tag="ACF3T5_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          553 a          218 c          242 g          498 t
ORIGIN      
        1 ataaatgttt gtcatactac gatttatcgt tgggtgcaag agtacagcaa agtcctctat
       61 tatctttgga agaagaaaaa tagacaatcc ttctattcat ggaaaatgga cgaaacctat
      121 atcaaaatta agggacgttg gcattatctt tatcgtgcaa ttgatgcgga cggcttaacc
      181 ttagatatct ggttacgaaa gaaacgggat acgcaagcag cctatgcttt cttaaaacga
      241 ctccataaac agtttggtga gccgaaagca attgtgaccg ataaagcacc ttctcttggc
      301 tccgccttta gaaagttaca gagtgtgggt ttatatacta agacagagca ccgaactgtg
      361 aagtatatga acaatttaat agaacaagac catcgaccta ttaaacgacg aaataaattt
      421 tatcaaagtc tccgtacagc ctcttccacg attaagggca tggagaccat tcgaggaata
      481 tataaaaaga actgaagaaa tggaacgctc ttcggctttt cggtgtctac tgaaatcaag
      541 gtattaatgg gaataacagc ctaagatatt tggagttcag agagggcgcg tttgattttc
      601 aaacttcgca acagaaccca ttagcaatat ataaaagaag gtatcaagag tcacagtgaa
      661 tttattccga aaagtgaaaa gagcaaatat aaaatttaat aaaaataaga actaaatagg
      721 aaaacgtaga cgtaattttc tacgttttcc ttttcatata tattaaatat tacggaaaat
      781 tatataaact aatttagtgt tttgtttgta attcctaatt ttgctccact tttagataat
      841 ttttaggtct atttaactca ttagacattc tatgctcatt tttctccaaa tttgtttcaa
      901 ctctttcgta cttaattttg cgtatttgaa aattcttctt ttagagtact tttattacga
      961 tactagtatg atatatatct acagcaaaca gttaaaatct tcctaaaata agcgaatagc
     1021 attcctaaaa cctataaaat ttttagatga agcgacaagt ggacaattaa taaactactt
     1081 gaacatgaaa ttatggatca cctttcaaat ttagaaaatg gaattcttat acttattgcg
     1141 caacatttat tgattgcaaa attatgtgac aaaaagaact agtagaagaa ggaataatgg
     1201 cttgataata aagtactacc tattaaaggt agtactttat ttaatttagt ataaattaac
     1261 atactactat attttaggtt tcatattata taaattaata atatactacc atttgttggt
     1321 agtataattt aaaggtaata agttattctt ttattactat gtttgggttt tatagcaaag
     1381 aaagtttaat aagagtttgg acctagcaaa caatattaat gaggtcatta aatcttatta
     1441 tctttagtga tataatgaaa tatctacacg acaaaattat gatcttgcgt aaagttataa
     1501 aatattaata a
//
LOCUS       DAXLPI010000239         1485 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.248, whole genome shotgun sequence.
ACCESSION   DAXLPI010000239 DAXLPI010000000
VERSION     DAXLPI010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.248"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            850..>1485
                     /locus_tag="ACF3T5_002785"
     CDS             850..>1485
                     /locus_tag="ACF3T5_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFD3633284.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFS"
BASE COUNT          490 a          245 c          250 g          500 t
ORIGIN      
        1 cgaaccgcca caagccacca aataaattcc agaaatctct ccatcatttt tttctacaat
       61 tgcgttaacc gtttttgcta catccatcaa catttcctcc ttaaaatttt aaaaatgtta
      121 attttatcca aaataaaagt aactgattaa atgtaaagat atcacataac gtcatataac
      181 gtcatgtgac ataattgcat tgtatccgct ttctttgtgt ttgtcaatag gaaaaattta
      241 tttctctaaa aaatataaat aatctgcagt gaaacttgag tacatatact aatttcgaaa
      301 aatgcctatg atttaaataa ttatggtgct gggatgaagg tttataagtt tgtcttgatt
      361 tgtttagaag aaatcgtttt tgcacacagt tgaattagga aactactgga gtggaaaata
      421 aagtgatttg acgggtagat gggtgcactt aaatttgaaa atggcttaaa atgctttatt
      481 ttgaattgca gaatttcttt tagtacgcaa tttatggtct taaaactgat taaaaagtga
      541 tacattgatt ttaatacaat ttatgataca aaaaatgacc tcctttctta aataaaagag
      601 gtcattttgt tttgtctcat aatcgattgt ttttgataca gtagttatat tcagaaaaaa
      661 taaagactct tctgagttta taatcctttg gattatatac ggatacagat gtagacataa
      721 aacaagctca attttcaaaa atggcattac tcaccttttt ggtgaattat ttgtataaaa
      781 aaaatataga aaagacaccc tgaaatcctt tagaattaat ttggctacca attcatagga
      841 ggattcaaga tgtctcatac tcagcttatc aaagataccc taaatattct agacctaaat
      901 atctatttcg aggaaaattg tttaactaaa gaaaaataca aagggcaaat ttgcttgatt
      961 tacaaaggaa ctcttctgta caagccagaa gagtgttccc attgtctttg tgtcgtcccc
     1021 tctaggatca ttcgttgggg gacgacaacc gttcggttgt tactgaatga tgtgtctgag
     1081 tatcgcactt atctggagct gaagaaacaa cgatttaaat gtaagtcttg ccaaagaaca
     1141 ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca ttagtcaaaa agttcgttgg
     1201 tctgtcattg ctcgcttaaa agagaatact tccatgacgg aaatcgctcg tcagaaaaat
     1261 atttccactt cttcggtcta tcgtgtcatg aagcgattct accgcccatt gaatcctttc
     1321 aaacagactt tgccaaaggt cttatgcttt gatgaattca aatcggttcg aagtgttgcc
     1381 agtgcaatga gttttatcat gatggacgga caaagccatg cattattgga tattgtcgaa
     1441 aatcgacgct tgccttattt agaacgctat ttttctcggt tttca
//
LOCUS       DAXLPI010000240         1478 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.249, whole genome shotgun sequence.
ACCESSION   DAXLPI010000240 DAXLPI010000000
VERSION     DAXLPI010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.249"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..1398
                     /locus_tag="ACF3T5_002786"
     CDS             <1..1398
                     /locus_tag="ACF3T5_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFD3633285.1"
                     /translation="GEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGN
                     YEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLP
                     ALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYN
                     PEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGS
                     SDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEK
                     VDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEP
                     NAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSS
                     NKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLG
                     TLGLLGYKNRKKENE"
BASE COUNT          582 a          230 c          324 g          342 t
ORIGIN      
        1 ggcgaaaaag tagaagttgg gggcacagta gatctaacag ataatgttac taacttgcca
       61 gcgttaccag aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac
      121 acaccaggta attacgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg
      181 gtgaaagttc cagtagaagt gacagacaat cgttctgacg ccgataaata tgaaccaaca
      241 gtagaaggcg aaaaagtaga aattggaggc acagtagatt taacagataa tgttactaac
      301 ttgccagcgt taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat
      361 accaacacac caggcaatta tgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa
      421 gatacggtga aagttccagt agaagtgaca gacaatcgtt ctaatgcaga taaatacaat
      481 cctgaaggac aaaaagtgac tacagactta aataaagagc cagatgcatc tgagggaatt
      541 aagaacaaag aagatttacc aaaaggaact acttatgctt ggaaagagaa agtagatgtt
      601 agcacagctg gaaataaaaa aggtactatt gtagtgacat atccagatgg atcatctgat
      661 gaagttgaag tagacgttac agtgacagat actcgttccg atgcagataa atataatcct
      721 gaaggacaaa aagtgactac agacttaaat aaagagccag atgcatctga gggaattaag
      781 aacaaagaag atctaccaaa aggaactact tatacttgga aagaaaaagt agatgttagt
      841 acagctggaa acaaaaaagg tactgtagta gtaacatatc cagatggatc taaagaagaa
      901 gtagaagtta ctatttctgt agtagataaa aaagcaccga ataaacctca agttgatcct
      961 attacagagg gtgaccaaat tgttactggt aaaactgaac caaatgcaga ggtgacagtt
     1021 acattaccag acggaagtca acaccatggg acagctgata aaaatggtaa ttttacagtt
     1081 aaagttccta aattagaggc agggacaaaa gtgatagtaa ctgcaactga tgaatctggt
     1141 aatactagtg aacctactaa tgtagttgtt tcttccaaca aaaaagacag tggaaaaaac
     1201 ggtagtaaag gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga caaaaataga
     1261 ggcaaaagcc agtcctcaaa agtttttcct aagacgggag agagtgacag taatatcttt
     1321 actattagcg gtggtttaat actgttagga acgttaggat tattagggta taaaaatcga
     1381 aaaaaagaga atgaatagaa aatcaatttc ttaagtcttc tctgagaaga ggaagaatct
     1441 ttttcttcct ctttttgctt ttttatgatg ggaaataa
//
LOCUS       DAXLPI010000241         1449 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.250, whole genome shotgun sequence.
ACCESSION   DAXLPI010000241 DAXLPI010000000
VERSION     DAXLPI010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.250"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>1449
                     /locus_tag="ACF3T5_002787"
     CDS             <1..>1449
                     /locus_tag="ACF3T5_002787"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HFD3633286.1"
                     /translation="IRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQ
                     QKGVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEASIKVNQGSFKGVLSPTQ
                     RFKTQEELLAFVSSKQAKIGNQEGIIADVQTKEKKTNSPSLFSLSSLQSKVNQLYKAT
                     ASQTLKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMVQTEPR
                     KRYVDGSKVQEHHAIIPTKQVPTESALAKMDDLQRKIYALVVKTTVAMFLPDYLYEET
                     KIQTKVADLLFQSIGKTPKQEGWKILFKQQTKEEKEDVQTLPLVIIGERAEVGVKSVE
                     KETQPPKAFTEGTLLTAMKTANKTVDDEEAIKILQEVEGIGTEATRASIIEALKQKEY
                     IQVIKNKLVVTEKGKLLCQAVESQHLLTSAEMTAKWETYLKKIGKREGNQENFITNIK
                     KFIVHLLEAVPNDIEKLNFSDYQEQKEKEAEKS"
BASE COUNT          549 a          243 c          293 g          364 t
ORIGIN      
        1 atccgaagcg gttttcaaaa tttgcaacct ggaatgaatt actatccctt ttatcaagaa
       61 gcgcaaacac gccaaattgc cgattggttg atcggcatga acgcaagccc tttgtatacg
      121 ttaaatttac aacagaaggg cgtacaaggt acattttcac taggacgtgt tcaaacgccc
      181 accttatacc ttatttttca gcgccaggaa gccatagaga attttaaaaa agaacctttt
      241 ttcgaggtgg aagctagtat aaaagtaaac caagggtcgt ttaagggcgt tctaagcccc
      301 acacagcgtt ttaaaaccca agaggagctt ttagcttttg tttcttctaa acaagctaaa
      361 ataggcaatc aagaggggat aattgctgat gttcaaacca aagagaagaa aacgaatagt
      421 ccgagtttgt tttctttaag tagtttgcaa tcaaaagtca atcagcttta taaagcgaca
      481 gcgagccaaa ctttaaaagc tatgcaagga ctgtatgaag caaaattatt gagttatcca
      541 agaacagata caccatttat tacagagaac gaatttgctt atttaaaagc aaattttggc
      601 aaatatagcg gttttttagg acttgatctc gaaatggttc aaacagagcc tagaaagcgt
      661 tatgtggacg gtagtaaggt acaggaacac cacgccatta tcccaacaaa acaagtacct
      721 accgaatctg cattagcaaa aatggacgat ttacaacgaa aaatatatgc tttagtcgtt
      781 aaaacgaccg ttgccatgtt tttacctgat tacttgtatg aagaaaccaa gatacaaacg
      841 aaagtagctg atttgctgtt tcaatcgatc ggaaagacac caaagcaaga agggtggaaa
      901 attcttttca aacaacaaac caaagaagaa aaagaggacg ttcaaacgtt accactcgtt
      961 atcattggcg aacgtgccga agttggtgtt aagagtgttg aaaaagaaac gcaaccgcca
     1021 aaagctttta cagagggtac attattaact gctatgaaaa cggcgaataa aacggttgat
     1081 gatgaagaag caatcaagat tttacaagaa gttgagggga ttggaacaga agcgacaaga
     1141 gcaagtatta ttgaagcgtt aaaacaaaaa gaatatatcc aagtgattaa gaataagctt
     1201 gttgtaactg aaaaaggaaa attattgtgc caggcagttg aaagtcagca ccttttaacg
     1261 agtgctgaaa tgacggctaa atgggaaacg tatttaaaaa aaatcggtaa aagagaaggc
     1321 aatcaagaga actttattac gaatatcaaa aaattcattg ttcatttact ggaagctgta
     1381 cctaacgata tagaaaaact aaatttttct gattaccagg aacagaaaga aaaagaagca
     1441 gaaaaaagt
//
LOCUS       DAXLPI010000242         1433 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.251, whole genome shotgun sequence.
ACCESSION   DAXLPI010000242 DAXLPI010000000
VERSION     DAXLPI010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.251"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..1244
                     /locus_tag="ACF3T5_002788"
     rRNA            <1..1244
                     /locus_tag="ACF3T5_002788"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1339..>1433
                     /gene="rrf"
                     /locus_tag="ACF3T5_002789"
     rRNA            1339..>1433
                     /gene="rrf"
                     /locus_tag="ACF3T5_002789"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          395 a          305 c          424 g          309 t
ORIGIN      
        1 agagaatcct aaggtgagcg agagaactct cgttaaggaa ctcggcaaaa tgaccccgta
       61 acttcgggag aaggggtgct gatcatacga tcagccgcag tgaataggcc caagcgactg
      121 tttatcaaaa acacaggtct ctgcaaaatc gtaagatgaa gtataggggc tgacgcctgc
      181 ccggtgctgg aaggttaaga ggagtgctta gcgcaagcga aggtacgaat tgaagcccca
      241 gtaaacggcg gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt
      301 ccgacccgca cgaaaggcgt aacgatttgg gcactgtctc aacgagagac tcggtgaaat
      361 tttagtacct gtgaagatgc aggttacccg cgacaggacg gaaagacccc atggagcttt
      421 actgtagttt gatattgagt gtctgtaccg catgtacagg ataggtagga gccgtagaaa
      481 tcggaacgct agtttcgatg gaggcgctgg tgggatacta cccctgcgtt atggccactc
      541 taacccgcac cactaatcgt ggtgggagac agtgtcagat gggcagtttg actggggcgg
      601 tcgcctccta aaaggtaacg gaggcgccca aaggttccct cagaatggtt ggaaatcatt
      661 cgaagagtgt aaaggcagaa gggagcttga ctgcgagacc aacaagtcga gcagggacga
      721 aagtcgggct tagtgatccg gtggttccgc atggaagggc catcgctcaa cggataaaag
      781 ctaccctggg gataacaggc ttatctcccc caagagtcca catcgacggg gaggtttggc
      841 acctcgatgt cggctcgtcg catcctgggg ctgtagtcgg tcccaagggt tgggctgttc
      901 gcccattaaa gcggcacgcg agctgggttc agaacgtcgt gagacagttc ggtccctatc
      961 cgtcgcgggc gttggaaatt tgagaggagc tgtccttagt acgagaggac cgggatggac
     1021 ttaccgctgg tgtaccagtt gttctgccaa gggcattgct gggtagctat gtagggaagg
     1081 gataaacgct gaaagcatct aagtgtgaag cccacctcaa gatgagattt cccatttctt
     1141 taagaaagta agatccctga gagatgatca ggtagatagg tcaggagtgg aagtacagtg
     1201 atgtatggag cggactgata ctaatcgatc gaggacttaa ccaataggtt aaaagaaaag
     1261 aactcggaaa aaaacttatg tgacttcaaa tccagttttg agtgaacaaa atttactcaa
     1321 taaaagataa caccacagtg tggtggcgat agcgagaagg atacacctgt aaccatgccg
     1381 aacacagaag ttaagcttct tagcgccgat tgtagtgagg ggttgcccct tgt
//
LOCUS       DAXLPI010000243         1364 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.252, whole genome shotgun sequence.
ACCESSION   DAXLPI010000243 DAXLPI010000000
VERSION     DAXLPI010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.252"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            82..>1364
                     /locus_tag="ACF3T5_002790"
     CDS             82..>1364
                     /locus_tag="ACF3T5_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFD3633287.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DAXLPI010000244         1361 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.253, whole genome shotgun sequence.
ACCESSION   DAXLPI010000244 DAXLPI010000000
VERSION     DAXLPI010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.253"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..486
                     /locus_tag="ACF3T5_002791"
     CDS             <1..486
                     /locus_tag="ACF3T5_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFD3633288.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
     gene            complement(693..1217)
                     /locus_tag="ACF3T5_002792"
                     /pseudo
     CDS             complement(693..1217)
                     /locus_tag="ACF3T5_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          445 a          254 c          260 g          402 t
ORIGIN      
        1 ttaaaggaag cgatcgatca aatcaagacg gctgtcttgt atcctcataa cggattaccg
       61 attttattga tgggtgtaag cggttcagga aaaacctttt tggcagaaaa aatttatgaa
      121 tacgccaagg aacaacaggt gatagaatca aatgctccat ttatcgccta taattgtgct
      181 caatacttta atttctcgat taacaagatc gtagaaaatt taacgatcct tgatgcaatg
      241 aaggttttga gattggtaca aaaggccgtc aaagaactcg aaaaactaat ggggatcgaa
      301 ttttcaaaca atcaattgtt cttgctttac atgcactgct gctcaatgat cgaacgtatt
      361 ttacgcaaag aaagtgtaga tgaacaagca gatatcaaag aatatattca aaaagaggga
      421 cataattatg aaatatcaaa atacccggct tacaaaacga agtttgaaag tactgtaata
      481 aagtaagatt taaataagca taaacaaggt aagaaccata ggggctgtgt caagtccaaa
      541 atcttgtgta aaaaggaaat ctcaaagtac gatgttttgc cttgttgagc ctagctacgg
      601 tccaatatgc tggtaagaag tgtcaagagc aaaaagcgaa gcgtctcttg atacttctca
      661 ccagtatatt atactattgg cggcttaatg tgctaaatct cgttacgatc aaattcaaga
      721 tattttctag gtgcagaagt ccattcgtca tccttttcca tcaataaagc accgatgata
      781 cgaataacag aatcacaatt tgggaaaatt cggacaacac gttcacgacg gcgtacttct
      841 tcatttagtc tttcaagaac attcgttgtt ctcaatcgtt gacggatatt ttctggtaaa
      901 accattactt gcatggcatc ttcgaagccc tcatccaata ttttacaggc agttgcgact
      961 ttcttgtctt ccccatattt atccataata agttgtttac gttctccagc cagctcgatg
     1021 cttgccgttt tgaagatggc tttaaagtct tctttgatat cgcttgagat tttcttggag
     1081 aatttgtcgg aaatgttgcg aaggagatgt gcttggcatc tctgctagct cgcacaaaga
     1141 aggtattctt tcacagcttt tgtgagccct atatgcgcat ccgagatgac catctttact
     1201 cctgaaagac cgcgttcttt caatccgaga gtgtaaaata ttttgtgtaa atagaaaaaa
     1261 ggaagtccct tcagtagaat agagttacca caacacattc acagaaaaga ggacttccat
     1321 atgaacgatt ttactacaga aattctaaag actctagcga a
//
LOCUS       DAXLPI010000245         1360 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.254, whole genome shotgun sequence.
ACCESSION   DAXLPI010000245 DAXLPI010000000
VERSION     DAXLPI010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.254"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            35..1308
                     /locus_tag="ACF3T5_002793"
                     /pseudo
     CDS             35..1308
                     /locus_tag="ACF3T5_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          447 a          279 c          240 g          394 t
ORIGIN      
        1 gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc ttatcaaact
       61 attactagga atcaaagatg gaaacattca aatcactcat gtcgagaaga aaaaaataca
      121 ttcaatcaac agtatcgttg tttctgccac gctcattaaa acgattcagc cctgtcctca
      181 gtgtaaacag gtgaatcaac atgggctgat cgttaagaac ggaaagaaaa aatcgctggt
      241 ccttctcaat aagtgtgcga atcaacggac ttatcttgct ttggcaaagc aacggtacct
      301 ctgtcgtggc tgtcacacct attttacggc gaatacctac attgttgaaa aaaactgttt
      361 tatcgccaaa caagtacatt ataaaatatt ggaggaattg actgaaaaac aagcaatgac
      421 gacgatttct aaacattgcg gcgtttcttg gtcaaccgtt tcgcgaacac tcgcttctct
      481 gctccccatg acaaacgtga aaataaattg gttgcctcgc tgttcattgg tagatgaatt
      541 tcgttcattg aaaaatcaag tcggtcccta ttctttttct tgtatggatg gcgatactgg
      601 gaaattactg gatattcttc cttcacgtaa gaagaaagat ctcatttctt attttatgca
      661 atttgaacga cgtgcacgtt taaacgtaaa aatactcgta acagatatga acgcctctta
      721 tgctttgctg ataaaagagt gctttcctca tgccaaactc gttgtggatc gctttcacat
      781 cgtcaagcac ctcattcgca gttttaagga tatttgacta tgagtcatga aaagcttcga
      841 ccgaaacgat ccaatacaag ccaaacacta tcgacaagtg aaggctttat ctcgacttct
      901 gatcaaacga caggacacgc ttgtttatga taaatggaca aaatggcgta attttggttg
      961 ggcctattta accgaaagtg aagtcgtaga acgtctactt tctacttctg atgaactacg
     1021 aatagcttat gcttactatc aagaaattct tcaagctttt tatgacaaag aagcggacat
     1081 cttctttcag cttgtaaaaa caatgcccaa gtcggttcca agagaactgc atcatataaa
     1141 aaaagcgttt ataaactatg aatccggcat tcgtttagcc ttagagttgc cttactcaaa
     1201 tggcaaaata gaaaatctgc atacccacat caaagcattg aaacgaatag cttacgggtt
     1261 aggatgagaa ccagaatttt cttaatcaat catctgatca cttattaaac ataaaaaaac
     1321 atctaaagca gaaaatcaat tctaccttag atgttcttat
//
LOCUS       DAXLPI010000246         1323 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.255, whole genome shotgun sequence.
ACCESSION   DAXLPI010000246 DAXLPI010000000
VERSION     DAXLPI010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.255"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            230..1321
                     /locus_tag="ACF3T5_002794"
     CDS             230..1321
                     /locus_tag="ACF3T5_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFD3633289.1"
                     /translation="MLELNTSLSNRTILITGAAGFIGSNLVLELLRTQSSIKIIGIDN
                     MNDYYDVSIKNYRLSEIETEAKNHPGSTWNFIKGSIADKATIDFIFDNYKPSIVVNLA
                     AQAGVRYSITNPDMYIESNIIGFYNILEACRHSYDNGESGVEHLVYASSSSVYGTNKK
                     IPYSTEDKVDNQVSLYAATKKSNESMAHAYSKLYNIPSTGLRFFTVYGPAGRPDMAYF
                     GFTDKLRAGDNIQIFNYGNCKRDFTYIDDIVEGVKRVMQCAPEKIDGEDGLPIPPYHI
                     YNIGNNTPENLLDFVDILQQELIAAGVLPEDYDFESHTELVPMQPGDVPVTYADTSAL
                     ERDFEFKPSTSLRDGLRKFAQWYKSFYEV"
BASE COUNT          451 a          195 c          250 g          427 t
ORIGIN      
        1 tttttctttt aaggttactt atgtgatcaa gaataatttc tattcgtttt agaataagtt
       61 aatagataat agaaagaagt gggttgctta gcaatttcct atctgtataa ttattctact
      121 acgtgcaaag attagaatga taaaagtgac aatagataag tctaaataag attttttaat
      181 atataacaaa tatgtattca taagaaaaaa agaaagtgag tgctaaaaaa tgctggaatt
      241 aaacacgtcc ttatcaaata gaacaatatt aataactggc gcagccggtt ttattggatc
      301 aaatttggtt cttgagttat tacgtactca gtcatcaata aaaatcattg gcattgacaa
      361 tatgaatgat tactatgatg tctctattaa gaactataga ttatcagaga tagaaacgga
      421 ggcaaagaac catccaggat ctacttggaa ttttatcaaa ggttctattg ctgataaagc
      481 aacaattgat ttcatctttg ataattataa accaagtatc gtggtgaatt tagcagctca
      541 ggcaggagtc cgttattcaa ttactaatcc agatatgtat atcgaatcta acataatagg
      601 attctataat attcttgaag cttgtcgtca ttcatatgat aatggagaat ctggtgtgga
      661 acatttagtg tatgcctcat cgtcttccgt ttatggtact aataagaaaa ttccttattc
      721 tacagaggat aaagtagaca atcaagtatc gttgtatgca gcaactaaga aaagtaatga
      781 atcaatggca catgcatatt ctaaacttta caatattccg tctacagggt tacgtttctt
      841 tacagtatat ggtcctgctg ggcgtccaga tatggcttat tttggtttta ctgataaact
      901 tcgtgctggt gataatatcc aaatattcaa ttatgggaat tgcaagcgtg actttaccta
      961 tatagatgat attgttgaag gagtaaagag ggttatgcag tgcgctccag agaaaattga
     1021 tggggaagat gggctaccaa tcccaccata tcatatttat aatattggta acaatacgcc
     1081 ggaaaaccta ctagattttg tagatattct acaacaagag ctaattgcag caggggtgct
     1141 accagaagat tacgactttg aatctcatac cgaactagtt ccaatgcaac caggagatgt
     1201 gccagttact tatgcagata ctagtgcgtt agaacgagat tttgaattta aaccaagtac
     1261 aagtttgcgt gacgggcttc gtaaatttgc gcagtggtat aagtcttttt atgaagtata
     1321 att
//
LOCUS       DAXLPI010000247         1296 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.256, whole genome shotgun sequence.
ACCESSION   DAXLPI010000247 DAXLPI010000000
VERSION     DAXLPI010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.256"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            74..697
                     /locus_tag="ACF3T5_002795"
     CDS             74..697
                     /locus_tag="ACF3T5_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFD3633290.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            complement(694..>1296)
                     /locus_tag="ACF3T5_002796"
     CDS             complement(694..>1296)
                     /locus_tag="ACF3T5_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633291.1"
                     /translation="ILKLISYAKKANSLSLVDNLDNLLPDKLIYDENEDNSQKQIIIF
                     ELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPFFTLINFMLYVKEHLGYDD
                     LVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYKEQNIKTLELIKKNIKYYD
                     PLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF"
BASE COUNT          464 a          198 c          198 g          436 t
ORIGIN      
        1 ctttatagtg tataaaaaaa ttagtgttga ttttgagtgt attttaaata gtttgaggaa
       61 gtaggtaaaa attttgaaaa ttgcttatat tcgagtttcg tcgattgatc aaaatgagca
      121 acgccaaatt gaagaaatga aaaagttcgg cgcagaaaaa attttcatcg agaaacaatc
      181 tggagcaacg attactcatc gaccgatttt ccaggaagct ttagagtttg tccgagacca
      241 agatattttt attgtcgaag caatcgaccg acttggaaga aattacgacg aaatcattgc
      301 ttcagtcaat tacctaaaga agaaaaacgt gcagctgatc atcaccagtc taccgatcat
      361 ggctgaagca attggcaatc ctcatttgga taaattcatt aaagatttaa tcatccaaat
      421 tttggccatg attgcagagc aagagcgtac cgaatctaaa cgacgccagg cgcaagtcat
      481 taaaattgca aaagcaaatg gcgtctataa aggtcgtcca aaactctata gtgcagatac
      541 aaaaaatcct caacgacgtt tagtttatga aaacatcgtt aaggatttaa atgagggcat
      601 agctatttca aaaattgcta aaaactacaa tattactaga caaactattt acagaattaa
      661 gaatgatatt gagtataaca gtaatcaaat gaattaaaaa agttctttat acattaaaat
      721 ttcttctttt aactcatcaa ttctaggaga tctaacatca gaatcagcaa ttgcatttaa
      781 taacttatca atattttctt tattcgctag taaaggatca tagtatttta tattcttttt
      841 aattaattcc agagttttta tattttgttc tttgtaatta gctagaattt cttctacagt
      901 ttcgcctgaa atcatagcca tatctgaacc agtattgatt cctttgaatg gataaggttc
      961 tttatcatat gcctttatca tttctactaa atcatcatat cctaagtgtt ctttaacata
     1021 cagcataaaa tttattaatg taaaaaatgg cattgaagaa tagtctggaa atataaattg
     1081 aaacagtttg ttcgcaaaat atttatcatc atcattgctt tctggtgaca ctgacaggct
     1141 gctaagccac tttaaataca attcaaatat aattatttgt ttttgtgaat tatcttcatt
     1201 ctcatcataa ataagtttat cgggtaaaag attatctaag ttatctacta aagataggct
     1261 attagctttc ttggcataag atattaattt taaaat
//
LOCUS       DAXLPI010000248         1294 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.257, whole genome shotgun sequence.
ACCESSION   DAXLPI010000248 DAXLPI010000000
VERSION     DAXLPI010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.257"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            256..1044
                     /locus_tag="ACF3T5_002797"
     CDS             256..1044
                     /locus_tag="ACF3T5_002797"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633292.1"
                     /translation="MEKINESKVSVCKEKGESYFLETLSLKQQEDKLFSEWEKEECLK
                     GYYGINQDGLADESSYLQSLPKIAFILKEAYGKENFDMRPYYYDGAHAGSKTWRTAPL
                     WIAGISKIYEENKSTIAQIKTEKRNMGWKEFNQEYIRKVAILNLNKQCTTNGKLTDRK
                     YFQSGIDNKKFINKQIEIYNPEIIICGGTFYPFYDRIYDYKNFDTKYESANQNKQYAE
                     GYKDKKGRLIINTFHPGAIKKEEPWYDSIMEIVERFKSEGLFAK"
     gene            complement(1130..1234)
                     /locus_tag="ACF3T5_002798"
                     /pseudo
     CDS             complement(1130..1234)
                     /locus_tag="ACF3T5_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          476 a          190 c          245 g          383 t
ORIGIN      
        1 ctcctggtat gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc
       61 ctatccagag tataatcatt gtaaggtcaa gtatttgact ttttcgatta tgagagcgtt
      121 accttttcta ttactgaagc ctttataaaa tggcgaaata atagaagaaa ctgaaagctc
      181 ttaataaata gacaaactac gttccaatag acgttttaaa aagggtatga aatacagcat
      241 acaaggagtg aaaaaatgga aaaaataaat gaaagtaaag tatcggtctg caaagaaaaa
      301 ggtgagtctt attttttaga aacgttatcg ttaaaacagc aagaagataa gctgttttct
      361 gaatgggaaa aggaagagtg cttgaagggg tattatggga taaatcaaga tggactggca
      421 gatgaaagta gctatttaca atcacttccg aaaattgctt tcatcttaaa ggaagcatac
      481 gggaaagaaa attttgatat gcgcccttat tattacgatg gtgctcatgc tggaagcaaa
      541 acttggcgaa ccgccccttt atggattgct gggatatcta aaatttacga agaaaataaa
      601 agcactatag ctcaaataaa aactgaaaaa agaaatatgg gttggaaaga gttcaatcaa
      661 gaatatatac gaaaggtagc tatcctaaac ctcaacaaac aatgtacgac taatggaaag
      721 ttaacggatc ggaaatattt tcaatccggt atagataata aaaaatttat aaataaacaa
      781 atagagatat acaatccaga aattattatt tgtggaggaa ccttctatcc gttttatgat
      841 cgtatttatg attataaaaa ctttgatacc aaatatgaaa gtgccaacca aaataaacag
      901 tacgcagaag gatataagga taaaaaaggc cgattgatta taaatacttt ccatcctgga
      961 gccataaaaa aagaagagcc atggtatgac tctataatgg aaatagttga aagattcaaa
     1021 agcgaaggct tgtttgctaa gtaagtgagt tccttcttcc aaattacaaa aaataatgga
     1081 taggctacac accttaattt ttctgtctag ttaattgtag cctatccagt cgcgcacgtc
     1141 ctaagcattg cgcagttttg gtaacggatt gattattttt gaaataagac tctatgacta
     1201 ctagttcgtc tgttgtaaga tgggtgtagg tcatttgtac tcactcctta ataattcttt
     1261 ggtcggaact attttgagtg tagcacaaat gatc
//
LOCUS       DAXLPI010000249         1272 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.258, whole genome shotgun sequence.
ACCESSION   DAXLPI010000249 DAXLPI010000000
VERSION     DAXLPI010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.258"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..582
                     /gene="mobL"
                     /locus_tag="ACF3T5_002799"
     CDS             <1..582
                     /gene="mobL"
                     /locus_tag="ACF3T5_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase MobL"
                     /protein_id="HFD3633293.1"
                     /translation="MNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVL
                     TKEPEYQEYIQISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQD
                     SQIKPLIESMDQMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLR
                     QLNRTINAHEEKYHASKAYEEQQRRIQMEQERQ"
     gene            603..938
                     /locus_tag="ACF3T5_002800"
     CDS             603..938
                     /locus_tag="ACF3T5_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633294.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            1084..>1272
                     /locus_tag="ACF3T5_002801"
     CDS             1084..>1272
                     /locus_tag="ACF3T5_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633295.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYPF"
BASE COUNT          502 a          192 c          264 g          314 t
ORIGIN      
        1 atgaacttat taaatgaagt atataaaacc ctacctgaca aacgttcgga atggcattat
       61 ggggatgaaa aacgaaccaa actttcacca tacacaagag agaaactcga tgagttaacc
      121 aaatatgtgt taacaaaaga acctgaatat caagaatata tacagatttc aaaatcattg
      181 aaagaagaag caaaaagtct ttatggggaa agcaagagag aatcaaagga tgcagaaaaa
      241 aatgctctct ttgatttgaa gaagcgcaca ggaaatgcca ttctaacaga actgaagaaa
      301 caggatagtc aaataaaacc tttaatagaa agtatggatc agatgcaatc tatttacaat
      361 gaacgatcct ttcgaacgct taaacagtta ccaacgaatg aatacaaata tagttacgaa
      421 gaattcatga agcgcaggaa acaattccaa caaagagttg tcacgcaacg gagtttgcgt
      481 caattaaatc gaacgattaa tgcccatgaa gaaaaatacc acgcaagtaa agcctacgaa
      541 gagcaacaac gaagaattca aatggaacaa gaaagacagt aaagacagta ggaggaatag
      601 caatgaaaca atatttagat caatggaaag ttatagaagg atcattaaga gaagaacgaa
      661 tcgaacaact tcccgattgt ttagaaaaag agcatttgtt tcagataaga gaaatgttga
      721 gaaacgaaca gtttgatcca aatcaatttt tagtcgttga gtactcagct actggtgtgt
      781 actgttgtaa tcatgtgaag ggtgaaaaat attttatcat tcaagaatat gagggcaagt
      841 tagcgcctta ttatactact tgggaaatga atgaagaggg gatcaataac ttcccgtgta
      901 aaagcattga agagtctatt tcactcacag aatgttagtt gttaagtgaa taaaagaagt
      961 aaaagagtcc tgaacgtaat tgtccaggac tctttttttc aatgaattgt tttccgccaa
     1021 ttcaatgagt ggtgcgattt gcgactacat tatatcaaaa gaaagagaag ggaggaagaa
     1081 gaaatggctt attcaacgat tggagttgtt gttgaaaaaa gcagagacaa tctcgttttt
     1141 gtgacagaaa ttcaaacggg cagagctttt gtcgtcacag acaaagcggc aaaagcgtat
     1201 caaagtggcg atattttaac gttaaatctg actacgaaga cgttcgtcga tgcggcagaa
     1261 gattatccat tt
//
LOCUS       DAXLPI010000250         1246 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.259, whole genome shotgun sequence.
ACCESSION   DAXLPI010000250 DAXLPI010000000
VERSION     DAXLPI010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.259"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            200..742
                     /locus_tag="ACF3T5_002802"
     CDS             200..742
                     /locus_tag="ACF3T5_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633296.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="ACF3T5_002803"
     CDS             898..>1246
                     /locus_tag="ACF3T5_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFD3633297.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAXLPI010000251         1235 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.260, whole genome shotgun sequence.
ACCESSION   DAXLPI010000251 DAXLPI010000000
VERSION     DAXLPI010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.260"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            35..532
                     /locus_tag="ACF3T5_002804"
     CDS             35..532
                     /locus_tag="ACF3T5_002804"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3633298.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..>1235)
                     /locus_tag="ACF3T5_002805"
     CDS             complement(569..>1235)
                     /locus_tag="ACF3T5_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFD3633299.1"
                     /translation="DLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIEIGYIAS
                     EKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIE
                     ADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLYDNLALY
                     EYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKRKRIMVP
                     LPV"
BASE COUNT          431 a          208 c          216 g          380 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatcg cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ttttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttctatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtca
//
LOCUS       DAXLPI010000252         1220 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.261, whole genome shotgun sequence.
ACCESSION   DAXLPI010000252 DAXLPI010000000
VERSION     DAXLPI010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.261"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..90)
                     /locus_tag="ACF3T5_002806"
     CDS             complement(<1..90)
                     /locus_tag="ACF3T5_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase"
                     /protein_id="HFD3633300.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQT"
     gene            89..937
                     /locus_tag="ACF3T5_002807"
     CDS             89..937
                     /locus_tag="ACF3T5_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFD3633301.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT          416 a          206 c          201 g          397 t
ORIGIN      
        1 tgtttgattt tttcctactc tccttgtatc tttaacatta atgagaacgg aaaaaatgat
       61 aatcttatta tgaaaatgag gaattctcat gagcgtctcc ataagactac aaatcaaaac
      121 aatgtacaac gacttcagcc caaaagaaca ggctatcgcc gattatattt tagaaaatcc
      181 aagcaaagta agtcatagtc ctattagcaa tttagcaagt gaactgggca ttgctgactc
      241 tacttttttt caatttacaa aaaaacttgg ttataatgga tttaaagatt ttaaaatggc
      301 catgttgatc caagaaaatg atctatctgc tatttctatc cacgaaaata ttcagaaaac
      361 agatactgag ctcactatgg cgcaaaaggt gttcgagtct agtatttcaa ctttaaatga
      421 cacaaaaaag ttgttacaac aaaaggatct aaaaaaagcg gctactatta tcaatgaatc
      481 taaacgtgtc tattttttcg gtgttggcgg ttcagagatt gtagcaactg acgcttatca
      541 taaatttttg cgttccccta tcgcaacttc tcacagcacg gattaccata ttcaattgat
      601 ggaagcttct ttattgacgg aaaaagactg tgctgttttg atttctcaca cagggcaatc
      661 aaaagaaaca atccatattg ctgaaaccgt aagaaaaact ggagcaaaaa caatcgttat
      721 tactagccaa gctaattctt cccttgctaa acttggcgat gtcgttttta tttctatttc
      781 tgaagaaaca gaattccgat ctgaagcttt agcttcccgt atttctcaac taagcatttt
      841 ggactctcta tatgtgattt tgatgtttta caacgaaaaa gaatcacgtc gttctatcgc
      901 taaagttcga catactattt ctgaaatcaa agaatagcac cataacagaa aaagcgaaaa
      961 atccataaaa caaaaaaagc cactaccaaa tatttggtag tggctttttt ggttctgcaa
     1021 gggtggtaca gaacatcaat agtcggtggg ttagaacgta tgtcatcgca tctttggaga
     1081 aggttgtatt ggtattattc gagttacttg ttagatgcga taacaaagtt agtatacagt
     1141 agtaaaaaat ttatcttttc ttttttttat tatttttgtt tcttattttc taaaaaatta
     1201 aaaaaaattt tggtgaatta
//
LOCUS       DAXLPI010000253         1198 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.262, whole genome shotgun sequence.
ACCESSION   DAXLPI010000253 DAXLPI010000000
VERSION     DAXLPI010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.262"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            357..440
                     /locus_tag="ACF3T5_002808"
     tRNA            357..440
                     /locus_tag="ACF3T5_002808"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:391..393,aa:Leu,seq:cag)
     gene            complement(764..>1198)
                     /locus_tag="ACF3T5_002809"
     CDS             complement(764..>1198)
                     /locus_tag="ACF3T5_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFD3633302.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          406 a          193 c          260 g          339 t
ORIGIN      
        1 atgatctcta gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag
       61 taatcaacag aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag
      121 aggaaaaaag aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa
      181 cattgaaatc gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga
      241 gttgctcgga tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag
      301 aacaaaaata tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg
      361 ctatggcgga attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag
      421 ttcgagtctt cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga
      481 tgagaaagaa aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc
      541 agattcaaat aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat
      601 tgattcaaat agtagattta tagattctga tacaagaaat actggcaaac gatagttaag
      661 gaataaagac taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct
      721 cttactatat tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg
      781 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
      841 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
      901 aacggccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
      961 tccattcttt tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag
     1021 caggttgtgt ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa
     1081 caggttgttc agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag
     1141 gttcagcagc tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DAXLPI010000254         1187 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.263, whole genome shotgun sequence.
ACCESSION   DAXLPI010000254 DAXLPI010000000
VERSION     DAXLPI010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.263"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(178..366)
                     /locus_tag="ACF3T5_002810"
                     /pseudo
     CDS             complement(178..366)
                     /locus_tag="ACF3T5_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            complement(528..>1187)
                     /locus_tag="ACF3T5_002811"
     CDS             complement(528..>1187)
                     /locus_tag="ACF3T5_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFD3633303.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSDGQKIDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          352 a          204 c          188 g          443 t
ORIGIN      
        1 gagagtattt tttttgattt tttattgttt caagaaaatc ggaataataa cttatgtact
       61 ttttaatttt gttaagtaat atatctagtt atatctagtt atatctagtt accttttctt
      121 tgaaaaaaac ataaatcagc taataaatat gtgagacaaa gctgccatcc ataaatacta
      181 gctgattttt tcagttaaat gtctattttt ttctctgatg atggtatttc caaaatattc
      241 ccagtcatct ggagtcggtg gatttttcca aattatctgt tctttattaa atttttcaag
      301 taaacaatca gataaataaa tattcgtacg agaagtgatt cgactctcaa tcacaatatt
      361 gtaatttgtg aactactcac cacttagcta aacctaacgg tttcttacgc ttgaagtggg
      421 agcttcttgg gaagagagca tacttgatag cgtattactt tactaacaga ggtgcctctt
      481 atttaccaag ctatccccgt agttcctacg gttcttgttt atttgatcta agccaatccc
      541 atagagtgta gccttagacc ttcggtcagg atattgatac tagcgttgat atctcggtca
      601 tgatgagtat gacaaatagg gcaagtccat tctcgaattt cgagagattt cttgccgtct
      661 ttatgtccac aattcgaaca gatttggcta gatggaaacc acttatctac tttgatgatt
      721 ttccgaccgt accagtcagc cttgtattgt aatttagaca caaaactaga ccaagaaaca
      781 tccgaaatac tttttgctag tttatgatta cgcaacatgc cttttgtgtt taagtcttca
      841 atacagatga tatcgtgatt tttgataatt tctgtactca acttattcag aaaatcagta
      901 cgttgattca ttactttttc atgcaatcgt gctactttac gtttttgtgt ttgataattt
      961 ctagcttcaa acagattgat acctttttgt ttagctaata aggcacgtct tgacaactta
     1021 cgttgttcac gtttgagttt cttttccatt ttggacgtaa atttattatt atcaattttt
     1081 tgtccatcag aaagaattgc aaagtctata atacctaggt caataccaac agcagaatta
     1141 gatttaggta gttcgcgaac ttcttctttg cataacaaag aaatata
//
LOCUS       DAXLPI010000255         1158 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.264, whole genome shotgun sequence.
ACCESSION   DAXLPI010000255 DAXLPI010000000
VERSION     DAXLPI010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.264"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..943
                     /locus_tag="ACF3T5_002812"
     CDS             <1..943
                     /locus_tag="ACF3T5_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFD3633304.1"
                     /translation="GAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAYQLVETQAP
                     TGYELDATPIEFEIERSQTAVVELTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDA
                     NGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVE
                     LTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLA
                     PGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSG
                     NKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT          420 a          198 c          288 g          252 t
ORIGIN      
        1 aggagctgtt tttgagttac agaaccgaga aggagaaacg ctacaaaccg gtctaactac
       61 aggagaagat ggaaaactgg caattgacgg tttagcacca ggagcatatc agctggtaga
      121 aactcaagcg ccaacggggt atgaattaga tgcgacaccg atcgagtttg agatcgaaag
      181 aagtcagaca gcggtggtag aattgacgaa agcaaaccga cttacaccag gcggtgtcgt
      241 gttgacaaag atcgatgacc aaagcgggga agtcctgcaa ggagctgttt ttgagttaca
      301 agatgccaat ggaaccgttc tgcagtcagg tctaaccaca ggagcagatg gaaaactggc
      361 gattgacggc ttagcaccag gagtgtatca gctggtagaa acccaagcgc caacggggta
      421 tgaattggat gcgacaccaa tcgagtttga gatcgaaaga agtcagacag cggtggtaga
      481 attgacgaaa gaaaatcgac ttacaccagg cggtgtcgtg ttgacaaaga tcgatgacca
      541 aagcggagaa gtcctgcaag gagctgtttt tgagttacaa gatgccaatg gaaccgttct
      601 gcagtcaggt ctaaccacag gagcagatgg aaaactggcg atagatggct tagcaccagg
      661 agcatatcag ctggtagaaa ctcaagcgcc aacggggtat gaattggatg cgacaccagt
      721 aactttcaag atcgaaaaag aacaagaagt ggcgatcttt ttaacaaaag agaatagaaa
      781 agttgctgat agctcagata taagaggaaa caatccaaca agcagtggaa ataagcatct
      841 tccaaaaact ggagaaacca tcattagcca gtttatattg tcaatattgg gtgtgctact
      901 tgttttcatc agtataaaat ttagaaagaa acgaaatata tgagctaaaa taaaaaattg
      961 aataagcata gtttttaaaa tcaactagta cccatcaatt gaatggaaac taccgatttt
     1021 agaaggggta tacaaacaaa tattagtatc ggagtagaga aggttgaacc caaccagtaa
     1081 ataagtaaag acaattttgt gctgcgttga gaagtgaaaa ataaaccttt gtagtaatac
     1141 tagttaataa aaaatata
//
LOCUS       DAXLPI010000256         1152 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.265, whole genome shotgun sequence.
ACCESSION   DAXLPI010000256 DAXLPI010000000
VERSION     DAXLPI010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.265"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..450
                     /locus_tag="ACF3T5_002813"
     CDS             <1..450
                     /locus_tag="ACF3T5_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HFD3633305.1"
                     /translation="KFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTTLFVSVIYF
                     ALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGLLPKFMKVA
                     DLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            459..686
                     /locus_tag="ACF3T5_002814"
     CDS             459..686
                     /locus_tag="ACF3T5_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633306.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          355 a          190 c          196 g          411 t
ORIGIN      
        1 aaatttgtcg ttttaggtat aagtgccgta tttattttgt tcggcgtact tttttatact
       61 cagatcgcta caagcgaaaa aggattcaat tttgattggc agaccacatt atttgtttct
      121 gtcatttact ttgcccttct cctagggatc gttattgctt atttcttatt gaaactattg
      181 aaaaaaatcg aagataacca agctttttcc actcaaagct taaacatact aaaaaaaatc
      241 gaacgaacca ttttgtttat tggtttcgcc ttttttggct tacttcctaa atttatgaaa
      301 gtagcggatt tagacgatgc tccaggtttg atgttggtag gaatcgctgt cgtattcatt
      361 ccttttgcag tatacacatt aacaagcgtg ctggaaaaac tgcttgaaca tgcgatttta
      421 cttaagacag atcatgattt gacagtgtga ggtgcgacat ggcaattatc gttaatttag
      481 atgtagtatt agctcaaaaa aaaatgagtg tgactgaatt gagtgagaaa gtgggcatta
      541 cgatggcaaa catctctatt ctaaaaaatg gaaaagcaaa agccatccgt ttttccactc
      601 tcgaaaaaat ctgccaagtt ttggagtgtc aaccaggtga tttgttgacc tataaaaatg
      661 actcgaatac tataccagaa gaataaaata aagtagaaca aacagtaaat aaaaaataat
      721 ctgaatgata gatgtgtgag acttttagaa gttatttacg tctattattc agatttttta
      781 tttcgattga ataccgtttt atctataaat aagatatgta ctgactcata caaagggggc
      841 cttttcttct ccatgtacta tcacttttcc tgatacaact ggtttcctgt tacgttgtag
      901 ttggatctgc ttttagtata atactttatt cattccacca gactgatagc agtttgagca
      961 gtctaaataa tcatgtagta ttacaggcca ttctgtttca taccttgaag tgcttaaaac
     1021 ttcagctaat ggcccattgt gttaactctt actccattct tttttagtaa gctcccatat
     1081 atactgtaaa gccttctata tattatgtca tgttgctttt taacatttac tatgtagttt
     1141 tgataaaatg at
//
LOCUS       DAXLPI010000257         1149 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.266, whole genome shotgun sequence.
ACCESSION   DAXLPI010000257 DAXLPI010000000
VERSION     DAXLPI010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.266"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            6..1149
                     /locus_tag="ACF3T5_002815"
                     /pseudo
     CDS             6..1149
                     /locus_tag="ACF3T5_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          484 a          184 c          256 g          225 t
ORIGIN      
        1 cggtcatggc agaagaaaaa gaaccactag acaatagtcg tttgggaaaa tccaaaagaa
       61 aattagtacg attgcaaaat gaattaaacg aacaaattga aaagatgttt gaacaccaga
      121 gaaagaccaa cggtcaacca atgaacgata agagaaatgg gcattcttgg tttaggcaac
      181 aagagcgaat cgaaaataaa gtgcatagtc tgagagaaga gatcaagcaa caagaaaaac
      241 aagtagaaaa acttgaaaga caagaagaga taaaggaaat gggctataac aagtacggcg
      301 gactggatat gacgatcgag aatattccaa gaatcaaaga agaaatcgaa cgctttgaaa
      361 aaggtgaatc gactttttca gccgctacga tccgaaaata ccaacggaaa ttggaaacat
      421 tagaacagct gaaagaaaga tcggaaaaag gaaaagaaaa ccttcttcct gaagtacaag
      481 caatcattga cagcggacga gtgacgcaat ggaaaaaaat ccgaccattt attttctgaa
      541 aggctatcga aaagtcgctt tagaactgaa tgaaaacggg gaatttgagg aatctaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctgt
      661 accaactgat tttttgaagg agaaagaaaa cgatcatttt tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacgttgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaagaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccca tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcatactc attctcctga
     1141 agacgtcaa
//
LOCUS       DAXLPI010000258         1137 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.267, whole genome shotgun sequence.
ACCESSION   DAXLPI010000258 DAXLPI010000000
VERSION     DAXLPI010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.267"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            322..591
                     /gene="rpsN"
                     /locus_tag="ACF3T5_002816"
     CDS             322..591
                     /gene="rpsN"
                     /locus_tag="ACF3T5_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFD3633307.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            606..785
                     /gene="rpmF"
                     /locus_tag="ACF3T5_002817"
     CDS             606..785
                     /gene="rpmF"
                     /locus_tag="ACF3T5_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFD3633308.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            817..>1137
                     /locus_tag="ACF3T5_002818"
     CDS             817..>1137
                     /locus_tag="ACF3T5_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633309.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLES"
BASE COUNT          410 a          153 c          235 g          339 t
ORIGIN      
        1 aagattgaat aaaaagttat agtttctttc atattgccgt tttaagtaga ggaaaagcta
       61 cttaaaacgg tatttgtgtt tgattggact aataaaagtg aattcttatt tttcagagcc
      121 taaactgcat attcttttaa atgatttgat tatctctatg taccgataaa ttcgttttca
      181 aatagttatc tattcatatg agttttattt catgttattt attgttgaca agagcaactc
      241 ttgttggtaa agtatcagtg tcaaaacgta acgattacga tttgaggtgt ctaaatcaaa
      301 ataaaaggag tataaaggta tatggcaaaa aagtcgaaaa ttgctaaagc aaagaaacaa
      361 atggcgatga ttgaaaagta tgcagataaa cgtcaggaat taaaagccgc aggagatcgc
      421 accgctctcg ctaaattgcc aagagattca aatccgaatc gtctgaggct tcgtgatcag
      481 acggatggtc gtccaagagg gtatatgcga aagttcggta tgtcccgtat taagtttaga
      541 gaacttgccc atcaagggtt gattcctgga gtaaaaaaag caagttggta aaaggaggaa
      601 atatcatggc agtaccagca agaaaaacat ctaaggcgaa gaaacggtta agaagaactc
      661 atcaaactgt agtaaaacca gaaatttcat ttgacgaagc gaatggtgat tatagacgta
      721 gccatcatgt atctttaaaa gggtactata atggaaagaa agtaattaaa ggagcttcaa
      781 aatagtttaa tatataactt ttaggaggga atcaaaatgg aaattattta cccaccttta
      841 gttgaagaag gattaaagta ttatcttgaa acgacccagc aatcgctaga taaatgcact
      901 ttttatcgtt cgatggtaga gagaggaatt attactgaaa cgggtttacc gacccaacag
      961 gcaattgaaa atggattagt caaagattac tatgaggacc aaggcttatc atttgatgat
     1021 tttttaagaa tctatccaat ttttgaagag tatgacgagg agttatttca atgtatcgat
     1081 ggttattggg agatacctgt tgatatgaaa gaaaatttag tttcacaatt ggaatct
//
LOCUS       DAXLPI010000259         1129 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.268, whole genome shotgun sequence.
ACCESSION   DAXLPI010000259 DAXLPI010000000
VERSION     DAXLPI010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.268"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            22..432
                     /locus_tag="ACF3T5_002819"
     CDS             22..432
                     /locus_tag="ACF3T5_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFD3633310.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            446..802
                     /locus_tag="ACF3T5_002820"
     CDS             446..802
                     /locus_tag="ACF3T5_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFD3633311.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          395 a          167 c          217 g          350 t
ORIGIN      
        1 taattaaaaa ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt
       61 agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt
      121 gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt
      181 caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa
      241 ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct
      301 attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt
      361 gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc
      421 ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt
      481 aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga
      541 cttagaagaa cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt
      601 gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct
      661 ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag
      721 acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc agttgatgga
      781 acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata
      841 cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat
      901 ttttctacta tagagacatg ctcaatttat agagacatgc tcaatttatg tggtcaaaat
      961 tcaaatctag gtggggatat agagttaaaa atatattgac atgtgtaaat ttacatgata
     1021 tattcatttc tgttgtttta agtagcaaag caataaaata gtattttaaa aaacatataa
     1081 aatagttgtt gacatgttgg aaatgacatg ataagataac tgagtcgtt
//
LOCUS       DAXLPI010000260         1127 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.269, whole genome shotgun sequence.
ACCESSION   DAXLPI010000260 DAXLPI010000000
VERSION     DAXLPI010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.269"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            25..900
                     /locus_tag="ACF3T5_002821"
     CDS             25..900
                     /locus_tag="ACF3T5_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFD3633312.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1045..>1127)
                     /locus_tag="ACF3T5_002822"
     CDS             complement(1045..>1127)
                     /locus_tag="ACF3T5_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HFD3633313.1"
                     /translation="NVEKYHKIYQQCRKIYPATKEICHDL"
BASE COUNT          396 a          215 c          199 g          317 t
ORIGIN      
        1 agtgtttcat gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt
       61 ttggaacgcg gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc
      121 aagtacttgt ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat
      181 gaaaagacca acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac
      241 atggctgctg tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt
      301 tccgaactct cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca
      361 gagattctta tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat
      421 gcaataaatt attcagataa attaacaact atcatccata aatacgatca tattatcaag
      481 aaaaacaaaa acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa
      541 gcagttgaat cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa
      601 gtgattgcta ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg
      661 atagaggact gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa
      721 aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa
      781 cgctactcca taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag
      841 aaaaaaatag ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag
      901 caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa
      961 acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt
     1021 tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta
     1081 gattttccgg cattgttgat agattttatg atacttttct acatttt
//
LOCUS       DAXLPI010000261         1126 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.270, whole genome shotgun sequence.
ACCESSION   DAXLPI010000261 DAXLPI010000000
VERSION     DAXLPI010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.270"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            2..244
                     /locus_tag="ACF3T5_002823"
     CDS             2..244
                     /locus_tag="ACF3T5_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633314.1"
                     /translation="MLGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHDVDRNEI
                     LEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(606..845)
                     /locus_tag="ACF3T5_002824"
     CDS             complement(606..845)
                     /locus_tag="ACF3T5_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633315.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          392 a          161 c          209 g          364 t
ORIGIN      
        1 cattttagga aaaaaatata tttgccaacc tattggcttg aaacaaaaag tggttggaga
       61 agtaatcagt aaattagaaa attgtgttgt gatctgcgta gacgaatatc atgacgtcga
      121 tcgcaatgaa atattagaaa aatgcggaaa agtagtggca aaatatactc acgtttatga
      181 gttggaaaca acagaatatc tatataacta tagcaacaaa tatgaacata ctttagtttc
      241 ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga aggcacttta
      301 tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc ctagagaatc
      361 tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc cagaactgtc
      421 ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa tcatttcaaa
      481 aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa gcatttgtaa
      541 aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg aagaagatga
      601 agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt ctttaccctg
      661 ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt tttctcattt
      721 gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat gttctttgat
      781 gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa cagaattctt
      841 ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc agtattaggc
      901 aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt aatacatata
      961 tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt attaacgatt
     1021 atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg tatgatatta
     1081 tctagaggta atttttttgg gtatttaaat agttgctagg aggata
//
LOCUS       DAXLPI010000262         1120 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.271, whole genome shotgun sequence.
ACCESSION   DAXLPI010000262 DAXLPI010000000
VERSION     DAXLPI010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.271"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..307
                     /locus_tag="ACF3T5_002825"
     CDS             <1..307
                     /locus_tag="ACF3T5_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFD3633316.1"
                     /translation="HHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGL
                     IALQEQDFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            337..459
                     /locus_tag="ACF3T5_002826"
     CDS             337..459
                     /locus_tag="ACF3T5_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFD3633317.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            477..>1120
                     /locus_tag="ACF3T5_002827"
     CDS             477..>1120
                     /locus_tag="ACF3T5_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFD3633318.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGY"
BASE COUNT          439 a          168 c          231 g          282 t
ORIGIN      
        1 acaccatcaa gtacatgagt ttaaatcaat tctcttaact tcagatcaag acataaaaaa
       61 agatttttta ctccaatgga ttgactggct gattttaacc aaccaaggga gcctgtatcg
      121 ttttaaagga ttgattgctt tacaagagca ggattttata gttgccatgc aagaggtaaa
      181 cgaacaggtt aattttcaat acaccactcg acaaaaagac caaaccacta ttattctaat
      241 cggaaaaatg ttggatgaag aaaggatcaa acagagtttt gataaattac tggcggaaag
      301 caaataatga taagtagtga ttgggggtaa tttctaatgg agaaaacgga tatatctagt
      361 gcatatcgac gacttaaaag cccgaatatt aaaactagaa aaagggcatt aaaaattatc
      421 aaagaacata aacgaaataa aatgaagaaa ttagcataat taaggaagga aaaagaatga
      481 agaaaatcag tgtaggactt attgggatag cagctctagg attattggga gcttgctcca
      541 gcactaatga tgcaaaagta tcgaatgata aagatggaaa attagaaatt gtaacaacat
      601 tctatccaat gtatgatttc accaaaaaca tcgtcggcga tgaagcgaac gtggatttaa
      661 tggttccagc gggatcagaa cctcatgact atgagccctc agctaaggat atggcaaaag
      721 ctcatgatgc tgatgttttt gtatatcata acgaaaacat ggaatcatgg gtaccaaaag
      781 ctaaagaaag ttggaagaaa gctgggccta atgtagtaga aggaacaaaa gacatgattt
      841 tattgccagg tagtgaagaa gaagatcatg atcacggaga agaagatcac catcatgaac
      901 tagatcctca tacgtgggtt tcacccaaaa tggcaattaa ggaagtttca aatatcaagg
      961 atcagttagt taaattgtat ccgaaaaaag caaaagtatt tgagacaaat gctgaaaagt
     1021 atttgactaa actgaaacgg ttggatgctg actataccac tagtcttaaa gaagcgaaac
     1081 aaaaaagttt tgtgacgcaa cacgcagcct ttggatattt
//
LOCUS       DAXLPI010000263         1106 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.272, whole genome shotgun sequence.
ACCESSION   DAXLPI010000263 DAXLPI010000000
VERSION     DAXLPI010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.272"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..330
                     /locus_tag="ACF3T5_002828"
     CDS             <1..330
                     /locus_tag="ACF3T5_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633319.1"
                     /translation="DFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLTNIENKGQQP
                     SFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVTIGKVIQAIY
                     GEKCKFN"
     gene            complement(844..1011)
                     /locus_tag="ACF3T5_002829"
     CDS             complement(844..1011)
                     /locus_tag="ACF3T5_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFD3633320.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
BASE COUNT          365 a          164 c          188 g          389 t
ORIGIN      
        1 gatttcaaac cattgggtct tgcgattaaa gaagcccgtc ttagtaaagg attgactcgt
       61 gagcaggtag gatcaatgat ccagattgcg ccacgctatt tgacgaacat tgaaaataaa
      121 ggacaacagc caagttttca tgttgtatat gatttagtga ctttgctaaa tatttctttg
      181 gatggattta ttttagggga agaaaacagt cataaaagct ccaaacgtct tcaagtagaa
      241 gcacagttgg attgtctgga tgaagatgaa ttagtgacga ttgggaaagt gattcaagca
      301 atttacggag aaaaatgtaa atttaactag ttcaataata cacaatggat taattaatac
      361 tatcaggaaa gattgattac aaagatgtaa tgaatttttt ctgatttttg ttaatcataa
      421 atagaaaatg aatttatctt tgttacactt aggaactaga gcgaatgata aatagttctt
      481 tggttactct tatcgctgca aagtacagtt cattttttgt tgttgggaaa attttctgga
      541 tttattgcat atcacttcat ccgactaaaa atttttgatt cccatagcga agttcgtttt
      601 tgttccttag gaaaaacttg actgatatac tagttttgaa atgacgagta actctgtact
      661 tcatcaataa tcaagtattg aattttaatt gtatactttt tatgcttgag ggatgtctac
      721 tgtaatacag ctactgcaaa aaaaatcagt acacttattt tcttataaag gatatcaaga
      781 actaagctca atggtatgca tagaaaagaa aaagccgata aaatgagatt ttgatatctc
      841 gaattatcgg ctctgcgtct ttgcgtctgg ctctgtaatt acagttattc taaacttcgt
      901 tatttcaatt aaaatttctt gtctgcattt aggacaaaat agagggaaat tttttaattc
      961 agtatcttcc ctcattttta aacgggtctt attgccacat atagggcaca gtatccacct
     1021 agtatttaca atatctatat cctcctgttc taattaacaa ctcaatattc attttaaaaa
     1081 tttctcttta gttcactatc aatgta
//
LOCUS       DAXLPI010000264         1089 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.273, whole genome shotgun sequence.
ACCESSION   DAXLPI010000264 DAXLPI010000000
VERSION     DAXLPI010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.273"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>1089
                     /locus_tag="ACF3T5_002830"
     CDS             <1..>1089
                     /locus_tag="ACF3T5_002830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFD3633321.1"
                     /translation="LFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIM
                     LDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEV
                     YLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQ
                     YSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSI
                     NRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTARL
                     LSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSL
                     AIEEQITLDKLKMLDYLFHPSFSQPYNFI"
BASE COUNT          416 a          132 c          184 g          357 t
ORIGIN      
        1 ttatttctag agacagaagt tgacaaaatt gatattcaaa ataaaaagat tagttgtgta
       61 aaaaaagatg gtacaagatt ctctcaagaa tatgacaaag ttatattagc tacaggttca
      121 aagcaaatta tgctagatat accggggaat aatttagcta atattttttc gataaagaat
      181 tttccaaatg caaaagagat ttttaaagct ttggatgata gtactattaa aaaagtagga
      241 attattggag caggatatat tggagttgaa attgctgaag ccataagaaa aagagggaaa
      301 gaagtatatc ttttcgatgt tgcagataga gtgttgtcga cttattacga ccgtagtttt
      361 tcagataaag ttgaagaaat actatcaaaa aatggaatac atctttgtct atcagaaagt
      421 cctaaaaaat atttaggaag aaaaaagatt gaaaaaatag tcactacaag tggtacccaa
      481 tactctatgg atatggtcgt acaagctata ggatttctat ctaatagtcc aattggggaa
      541 aaggaactct tgagagatgt tagtggagca tatctaacta atgaatacca gcaaacaaat
      601 ataaaagata tatatgctat tggggattgt gctacaactt tttttacgtc cattaataga
      661 agtagtgttg atttttctat ttctaatgct ctaagaacag cttatattgc aactcaacat
      721 attaacaatc aagattttac accttctggt tcccaattta caaatggctt ttctatattt
      781 aattacaatt tttacgcagc aggtttgaat ttaaaaacag cacgattatt aagtgagtct
      841 atagattata tagaaataga agatttcatt agacctgctt ttttgagaaa aaatagtcgt
      901 gtaaaattaa gaattagctt tgaaaaaaat acaaagagaa tagtaggtgt acaactttgt
      961 agtcaagaag atttatctgg actattatca atgttttctc ttgctataga agaacaaatt
     1021 acattagaca aattgaaaat gttagattat ttatttcatc ctagttttag ccaaccctat
     1081 aattttatt
//
LOCUS       DAXLPI010000265         1080 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.274, whole genome shotgun sequence.
ACCESSION   DAXLPI010000265 DAXLPI010000000
VERSION     DAXLPI010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.274"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(81..896)
                     /gene="thiD"
                     /locus_tag="ACF3T5_002831"
     CDS             complement(81..896)
                     /gene="thiD"
                     /locus_tag="ACF3T5_002831"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFD3633322.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT          279 a          238 c          177 g          386 t
ORIGIN      
        1 ttcaacaaac atgagaagct gttttttgaa cttgttcctt gtgactcgga gctggacggc
       61 aggctcacga cctctttctt ttactctaat tcaccagctg cccaatgatt gatgggtcca
      121 tatttgtgcc ctacatttat ttcttcacga attgcacggt ttacatagtc ttttgccaat
      181 cggatcgctt gttcaactgt ttttcctttc gctaactcag ctgcaataca tgcagatagc
      241 gaatcgccag ttccgttgat ccggtcggta ggataaaatg gtgcatttaa ccaaaacgat
      301 tttcctgatt cgagcaatac gtagtcattg actgtttctt gctgcgcatc tttagcatga
      361 tgtcctttga tcataacgtt ttttgcaccc atatcttgca gttcttttgc tgcttgcaca
      421 aaatcttctt cagctgctaa tgtccgtcca gtcagctttt ctgcttcata aaaattaggt
      481 gtcagtactt cggccaaagg gacaagcttt tctttcaagc tttgcaaagc agattcttct
      541 agtaaaagat ttccatgttt cgttacgatt acgggatcca cgactaatgg tccaaaatca
      601 tatttttggt aattttttac aactgtctca atcaactttg aatctgctag catacctgtt
      661 ttagctgcac gtatttgaaa gtccttcgca atcagttcaa attctttgtc gataaaatct
      721 gttggcaatg tcacacttgc tccgattccg taagaattac ctgcaacaca tgcggtcatg
      781 acagaaacac cgtaaacatt tcttgtaaag aatgtatgca tatctgcttg catccctgcg
      841 cttccatcgc tatctgatcc tgcgatcgtc attgcttgta catactgatc ttccatcaaa
      901 tcgattcctc catttttata aaaagatttt tcataatgct gttactgttt ctatcatatg
      961 tttttcaaag tatccctcag taaatatacc actacctttt cttggtaatc aaatttattt
     1021 gaaaaaacat ttgtaataaa tcagaaaata gttgtcattt gtttgtattc gctattctaa
//
LOCUS       DAXLPI010000266         1079 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.275, whole genome shotgun sequence.
ACCESSION   DAXLPI010000266 DAXLPI010000000
VERSION     DAXLPI010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.275"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..968
                     /gene="bsh"
                     /locus_tag="ACF3T5_002832"
     CDS             <1..968
                     /gene="bsh"
                     /locus_tag="ACF3T5_002832"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFD3633323.1"
                     /translation="SITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVNDLDT
                     HYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFIPWI
                     LGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHIYDN
                     PVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSV
                     SRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNV
                     DKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          379 a          134 c          185 g          381 t
ORIGIN      
        1 cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat tatgaaatat
       61 cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt cgaaaggtaa
      121 atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct gactaccctc
      181 tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat ttttctgggt
      241 atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt gaatttattc
      301 cttggatttt aggacaatgc tcaacagtag gagaaactaa aaaattgtta aaaaatatca
      361 atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat tggctattag
      421 ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat atatatgata
      481 accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt aatttaaaca
      541 attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa ataagtttga
      601 atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc tcagtatctc
      661 gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca gagtcagaaa
      721 gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt ttgtgtgatg
      781 ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt gacaaaggaa
      841 tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg aataaagaag
      901 acttagatag tcataagtta attagttatc caattataga aaaacaacaa attaaatata
      961 taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa aaaggcaaag
     1021 atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa tgtttttaa
//
LOCUS       DAXLPI010000267         1073 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.276, whole genome shotgun sequence.
ACCESSION   DAXLPI010000267 DAXLPI010000000
VERSION     DAXLPI010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.276"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>1073
                     /locus_tag="ACF3T5_002833"
     rRNA            <1..>1073
                     /locus_tag="ACF3T5_002833"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAXLPI010000268         1072 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.277, whole genome shotgun sequence.
ACCESSION   DAXLPI010000268 DAXLPI010000000
VERSION     DAXLPI010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.277"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..492)
                     /locus_tag="ACF3T5_002834"
     CDS             complement(<1..492)
                     /locus_tag="ACF3T5_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFD3633324.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYR"
     gene            complement(552..761)
                     /locus_tag="ACF3T5_002835"
     CDS             complement(552..761)
                     /locus_tag="ACF3T5_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633325.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(828..1067)
                     /locus_tag="ACF3T5_002836"
     CDS             complement(828..1067)
                     /locus_tag="ACF3T5_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633326.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT          294 a          241 c          140 g          397 t
ORIGIN      
        1 acgatagacg ccatcgttac taactaagtc catcacctcc cctaatgaca acttttgaat
       61 atcagaaaat aaaacacctg gcatttccat gttatgacct gctaggacat agtttccttt
      121 gcccatttcg gcatcttcat aaaaataagt agccgctcca agactcaaag tgtattgatt
      181 tgccccttta aaaattggca atgctaaacc aagtgcagga atctgaattt gtgcaaatac
      241 ttgtttattg acgcccattt cgattgcttc tttttgtatg gacaacattt gccccaaaga
      301 catatcatta ttgacgttcg ttgcatcctc cttataactc tttgcatgtg cattgatttg
      361 atctacgggc atcttttttt ctatattttg cttaaacacc tctccttcat gtttttggta
      421 gctaacatag ccaaacacac tgcaaaagac gattaaagtg actcctaaaa aaccaaataa
      481 ccattttttc atccatctgc ctcctttcag acaaaacaaa aaaacttttt ctcatttcca
      541 tagaaaaagt tttaaaaatg aattctataa gttcgtctca actgttcaaa gtttttcatt
      601 gaacctaaca aacaagcttt ctcttttcct gttaactgca tatcattgat atattgcgcg
      661 acagataatc ttttaaggtg tttttgcttc atcgttttta gtaacttttc ttccatttct
      721 ttcgacactt tttttctgtt ttcgatttct tcataattca tgagttttcc taccttcctt
      781 gttttttgca aaaataaaaa agcctatgat cacatacgct ctacacttca gtcttgggtt
      841 atctttttct ttcgtttaat tactggccgt gctggtttca aatccttgat tccattatgg
      901 gttctccaat aatcgaattc ttttcgcaat ctttcttttt ccatatctgt tcctttagat
      961 aaggctttga atacttcttt aggtgaataa ccacgagcta acagctgtac ataccttcct
     1021 tttgtcaaag tatccatatc catcgcctct aaaactggtc gcgccatcta ct
//
LOCUS       DAXLPI010000269         1068 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.278, whole genome shotgun sequence.
ACCESSION   DAXLPI010000269 DAXLPI010000000
VERSION     DAXLPI010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.278"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..294)
                     /locus_tag="ACF3T5_002837"
     CDS             complement(<1..294)
                     /locus_tag="ACF3T5_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFD3633327.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNL"
     gene            complement(294..>1068)
                     /locus_tag="ACF3T5_002838"
     CDS             complement(294..>1068)
                     /locus_tag="ACF3T5_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HFD3633328.1"
                     /translation="VIGIVSGTVGCFIILRGMSLMGDAISHAVLPGVAISYILGINFF
                     IGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSSFLALGVILIGVANSSTDLFHILF
                     GNILAVQDIDKWITIGVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMV
                     LLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIG
                     YSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
BASE COUNT          397 a          202 c          128 g          341 t
ORIGIN      
        1 caagttaaca ccgttataaa aaatcaaatc tgcttttgaa gttttttgaa catcttcagg
       61 caaaggttca tattcgtggg gatctttccc aataggtacg atactgtgta aatcaatttt
      121 atcttttgct atattttcgg taatatctgc taaaatcgaa ttagtaacta ctacttttaa
      181 tttttcgttc gaagccactt tatctttgtc tttactattt attgaacatg ccccgaatat
      241 aaaaagagca atcaaaatcc ccagacttcc cagtactgct ttccattttc tcatcataac
      301 gatctctcct tctttcttaa aaatttttgt tttggtgaga ttagaaaact aattacaaat
      361 aagaatgccg ctgttaaaac aatacttgat cctgctgcta tgttaaaact ataaccaata
      421 aacaatccaa gcaatgatga acttgctccg actgttgcag aaataaatat catagttttc
      481 aagctatttg catataaata tgctgtagct gctggtgtaa ttaacattgc aacaattaat
      541 atagttccca cactttgcat agctgttact gctactaaag ttaatagaac catcaataga
      601 taatgataaa aactgacatt catccctatt gcttttgcca ttaatggatc aaaagaagtg
      661 attaataatt ctttaaaaaa tagaactact ataaccaaca ctaaaaccga aacgccaatt
      721 gtaatccact tatcaatatc ttgtacagct aaaatatttc caaataaaat atgaaataaa
      781 tccgttgagc tatttgcaac tcctataagg ataactccta gtgctaaaaa agaactgaaa
      841 gtaataccaa ttgctgtatc tccttttatt acactattgc tttttataaa agtaataatg
      901 atagaagcta atagaccaaa tacaatcgca ccaataaaaa aattaatacc taaaatataa
      961 gaaattgcta ctcctggcaa aacagcatga gaaatagcat cacccataag agacattcct
     1021 cttaaaataa taaaacaacc tactgttcca gaaacaatcc ctataact
//
LOCUS       DAXLPI010000270         1065 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.279, whole genome shotgun sequence.
ACCESSION   DAXLPI010000270 DAXLPI010000000
VERSION     DAXLPI010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.279"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(39..>1065)
                     /locus_tag="ACF3T5_002839"
     CDS             complement(39..>1065)
                     /locus_tag="ACF3T5_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFD3633329.1"
                     /translation="LTGNPKNIRLFLYNLFNSTEISFVELYTLPIIIKTEKMLKDLKQ
                     YYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFI
                     NKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFENQFNLLREETSQLIRRELTK
                     IHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFS
                     YLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPD
                     VIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPE
                     EIIDRIE"
BASE COUNT          346 a          178 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaac
//
LOCUS       DAXLPI010000271          973 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.280, whole genome shotgun sequence.
ACCESSION   DAXLPI010000271 DAXLPI010000000
VERSION     DAXLPI010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.280"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(34..210)
                     /locus_tag="ACF3T5_002840"
     CDS             complement(34..210)
                     /locus_tag="ACF3T5_002840"
                     /inference="COORDINATES: protein motif:HMM:NF021542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFD3633330.1"
                     /translation="MKEFIEKYKYPLIFACVGLLLGLLILTLGLFKIVIITILTVCGF
                     YLGIYLETIGFFDK"
     gene            complement(220..780)
                     /gene="amaP"
                     /locus_tag="ACF3T5_002841"
     CDS             complement(220..780)
                     /gene="amaP"
                     /locus_tag="ACF3T5_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFD3633331.1"
                     /translation="MRKSLKLLLTIVLLLFFFPLAHTLITMQSVVSLPFYLLPFTSYP
                     YIGNNMAQFLFWLSAVLLTFLFIILIIVLFYPRINNYIEKKTDTGKIAIQKKAIENFV
                     LIAAKEEPFIVDPTVKVVIKKKKIKIYVRGKIRKAFQTGERQTSFIRKVNTNLNALFG
                     AEQTILTEIKFNDYHERNKETKSKVL"
BASE COUNT          333 a          147 c          113 g          380 t
ORIGIN      
        1 attttttacc gtaaaaacgg ttattaacta gttttatttg tcaaaaaatc caatagtttc
       61 taaatagata cctaaataaa aaccacatac agtaagtatt gttattatca ctattttaaa
      121 aagtcctaac gtgagaatta ataatcctaa tagtagtcct acacatgcaa aaatgagcgg
      181 atatttatat ttctctatga attctttcat tgttttctcc tataaaactt ttgatttggt
      241 ttctttattt ctttcatgat aatcattgaa ttttatttca gttagtattg tttgttctgc
      301 accaaataag gcattcaaat tagtgtttac tttccttata aaggaggttt gtcgctcccc
      361 tgtttgaaaa gctttacgta tttttccccg tacatagatt tttatttttt tcttcttaat
      421 tacaactttt accgttggat caacaataaa aggctcttct tttgctgcga ttaatacaaa
      481 attctcaatt gctttttttt gaattgctat ttttccagta tcggtctttt tttctatata
      541 attatttatc ctaggataaa ataatacaat aattaaaata ataaataaga atgttagcaa
      601 aacggcacta agccaaaaca aaaattgagc catgttatta ccaatatatg ggtaacttgt
      661 aaatggtagt aaataaaatg gtaaactaac cacactttgc attgtaatta acgtatgtgc
      721 taaaggaaaa aaaaacagca gtaaaacaat agttaatagt agtttcaagc tttttcgcat
      781 attccacctc ttagattacg ttgtgaatat aattctttat tttattattc tatggaactt
      841 tacaattaaa caaaacctat aaattaaact tgacacagat tttggcagtt tgatttctag
      901 aatattctcc tttcatttca tttatctcac taactttagt taaactaata cgataagttt
      961 aaccaattac tat
//
LOCUS       DAXLPI010000272          961 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.281, whole genome shotgun sequence.
ACCESSION   DAXLPI010000272 DAXLPI010000000
VERSION     DAXLPI010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.281"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            101..>961
                     /locus_tag="ACF3T5_002842"
     CDS             101..>961
                     /locus_tag="ACF3T5_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFD3633332.1"
                     /translation="MNVFDATLKRLEYIFEEFDKVYFSFSGGKDSGIMVQLANLVAEK
                     LDRNFDLLILNIEANYTATVDFIKKIEQLPRVKNIYHFCLPFFEDNNTSFFQPQWKMW
                     DPSEKEKWIHSLPKNAITLENIDDGLKKYYSLSNGNPDRFLRYFQNWYKEQYPQSAVS
                     CGVGIRAQESLHRHSAVTKGENKYKNRCWINITLEGNILFYPLFDWKVDDIWAATFKC
                     GLEYNYIYEKMYKIGYKYRDMRICQPFGLQQRKALNQFAELEPETWDKLVKRVSGVNF
                     GNIYGKTSLLG"
BASE COUNT          364 a          100 c          175 g          322 t
ORIGIN      
        1 tttgaatact ataagaaaca acaattgata aaattattga caaagttttt aaaggtatta
       61 ccattgatat tgaacgctta tttttttagg gggatgagaa atgaatgtct ttgatgctac
      121 tttaaagaga ttagaataca tatttgaaga atttgacaag gtgtattttt catttagtgg
      181 tggtaaggat agtgggataa tggttcagtt agctaatcta gttgcagaaa aattagatag
      241 aaattttgat ctattaatat taaatattga agcaaattac acagcaactg ttgattttat
      301 aaaaaaaatt gaacagttac ctagagtaaa aaatatttat catttttgct tacctttttt
      361 tgaggataac aacactagtt tttttcaacc tcaatggaaa atgtgggatc caagcgaaaa
      421 agaaaaatgg attcattctc ttcctaaaaa tgccataact ttggaaaata ttgatgacgg
      481 tttaaagaaa tattattcat tatcaaatgg taatcccgac agatttcttc gttattttca
      541 aaattggtat aaagaacaat atccccaaag tgctgtatca tgtggtgtag gtataagagc
      601 acaagaaagt ttacatagac atagtgctgt tacaaaaggt gaaaataaat ataagaatcg
      661 atgttggata aatataactt tagaaggaaa tattttgttt tatcctttat ttgattggaa
      721 agttgatgac atttgggctg caacttttaa atgtggacta gaatataatt atatctatga
      781 gaaaatgtat aagattggat ataagtaccg ggacatgaga atatgtcagc ctttcggatt
      841 acaacaaaga aaagcgttaa atcaatttgc tgaacttgaa cctgaaacat gggataaatt
      901 agtaaaaaga gtaagtggag ttaattttgg aaatatatat ggtaaaacta gtcttttagg
      961 a
//
LOCUS       DAXLPI010000273          927 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.282, whole genome shotgun sequence.
ACCESSION   DAXLPI010000273 DAXLPI010000000
VERSION     DAXLPI010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.282"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..645
                     /locus_tag="ACF3T5_002843"
     CDS             <1..645
                     /locus_tag="ACF3T5_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002907381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFD3633333.1"
                     /translation="GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPH
                     AWLNLENGIIYAKNIEKHLAEKDPDNKKIYKENLDKYIEKLNTLDKEAKSKFASIPND
                     KKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGTPDQIKHLVERLRTTKVPSLFVES
                     SVDDRPMKTVSKDTNIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            663..894
                     /locus_tag="ACF3T5_002844"
                     /pseudo
     CDS             663..894
                     /locus_tag="ACF3T5_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
BASE COUNT          388 a          113 c          175 g          251 t
ORIGIN      
        1 ggaaatgctt ggtttacaaa attagttaaa aatgcgaaca aagaggaaaa caaagactat
       61 tttgcagcaa gtgatggcat agatgttatt tacttagagg gtcagagtga gaaagggaag
      121 gaagatcctc atgcttggtt aaatttagaa aacggtatta tttacgctaa aaacattgaa
      181 aaacatttag cggaaaaaga tcctgataat aaaaaaatct ataaagaaaa tctagataag
      241 tatattgaaa aattgaatac actagacaaa gaagcaaaat ctaaatttgc ttcaattccg
      301 aatgataaaa aaatgattgt tacaagtgaa ggatgcttta agtatttctc gaaagcgtat
      361 aatgtgcctt ctgcttacat ttgggaaatc aacactgaag aagaaggaac gccagatcaa
      421 attaaacact tagttgaaag actacgcaca acaaaagttc cttccttatt tgtagaaagc
      481 agtgtggacg atagaccgat gaaaacagta tcgaaagata ccaatattcc tatctattca
      541 acgattttta ctgattcaat tgcagagaaa ggacaagatg gtgatagtta ctacgcgatg
      601 atgaaatgga acctggataa aattgctgaa ggtctttcaa aataaattgg aggaaaaaga
      661 gtatggtaaa aattaaacca actactatac tgatttattt attagcaatt caggaattga
      721 cgaaaagaaa aaaagttaaa aaataaagat ttagcaaaaa ttctaagcat tactccagca
      781 tctgtaagtg agatgttggc taaacttaga tatgaaggtt atttaaacat agggtgtcaa
      841 ttgacaaata gagggaaaga ctttattgac aattacaaaa agagctttat ataaaagact
      901 ttttatttag aaaggataag aaaatga
//
LOCUS       DAXLPI010000274          919 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.283, whole genome shotgun sequence.
ACCESSION   DAXLPI010000274 DAXLPI010000000
VERSION     DAXLPI010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.283"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            66..290
                     /locus_tag="ACF3T5_002845"
     CDS             66..290
                     /locus_tag="ACF3T5_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFD3633334.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(495..815)
                     /locus_tag="ACF3T5_002846"
     CDS             complement(495..815)
                     /locus_tag="ACF3T5_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFD3633335.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT          323 a          133 c          177 g          286 t
ORIGIN      
        1 gtctaatcaa aaaaatataa aaattatttt gcgtacatat gattaaagaa aggagagtgt
       61 gacacatgga aacagaatgg caaagacgtg aaagaaaaaa acagaaaaaa gaagagatgt
      121 tacgattatg tcgattatta gatgtaaaag actgggacaa aaacgagcaa attgcacaaa
      181 aagtacgatc aatcatccat aaagaggaaa aagttaaaaa taaagacacg cgtagaactg
      241 aaaatgaaaa gagaaaagta caaaagaaaa gagaaaaatc aaaaagctag aaagaaaaag
      301 aggctgagac agaagcgttt aactccaaga aataagctgg aattcacaaa aatttgaaaa
      361 gcaattttcg tgaatttcag cttatttccg aaggagttgc ttctgctttc gccgtttata
      421 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct ttaattatta
      481 tgttcaaaat ttgtttacac ggctaatgta gcgcctaatt catgttttgg aatcgagatt
      541 tcaacaaggc ctgccaattc gattactttg atttcatcct cgtggtaaac ggcgtctttg
      601 attttatcta agaaataatt tttagcatct tcgatttctt tcggctggat tttacgtttg
      661 tcattgatca gcagaatttc attatgttca ggggcttcag tgtagacaat agtggtattt
      721 ccggctgtgt agaccttgac tagatgggca tcagtgtttt caagctggtt taatacaaga
      781 cgggaatggc tgtttgttac atttaccaat ttcatggcgc tcacctcttt tatgtatttt
      841 taattgcttg tttctcttat tataatttct tttttataaa atgaacagta ttttgacttt
      901 tctgaaaaaa gaatgagac
//
LOCUS       DAXLPI010000275          912 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.284, whole genome shotgun sequence.
ACCESSION   DAXLPI010000275 DAXLPI010000000
VERSION     DAXLPI010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.284"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            136..309
                     /locus_tag="ACF3T5_002847"
     CDS             136..309
                     /locus_tag="ACF3T5_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633336.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT          304 a          147 c          110 g          351 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gc
//
LOCUS       DAXLPI010000276          908 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.285, whole genome shotgun sequence.
ACCESSION   DAXLPI010000276 DAXLPI010000000
VERSION     DAXLPI010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.285"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            40..>908
                     /locus_tag="ACF3T5_002848"
     CDS             40..>908
                     /locus_tag="ACF3T5_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFD3633337.1"
                     /translation="MIQYYYIKKEWGVVMEKEELKILEELRRILNSKNEAIVILNNYF
                     KGGVGKSKLSTMFAYLTDKFNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMLER"
BASE COUNT          337 a          129 c          159 g          283 t
ORIGIN      
        1 agtgattaat cacaaatcac ttgtgatttg tgattcctaa tgatacaata ttactatata
       61 aaaaaagaat ggggcgtagt tatggagaag gaagaactaa aaatacttga agaattaaga
      121 cgtattttaa acagtaaaaa tgaagcaatt gttatcttaa acaattattt taaaggtggt
      181 gttggaaagt caaaattatc gactatgttt gcttatttga cagacaaatt taatttaaaa
      241 gttttaatga tcgataagga cttacaagca acattgacaa aagacttagc aaaaacattt
      301 aaggtagaat tgccacgtgt caatttttat gaaggcttga aaaatggaaa cttggcttct
      361 tctattattc atttgactga taatttagac ttgatccctg gcacgtttga tttgatgtta
      421 ctgccaaaat taactcgctc atggacgttt gaaaacgaaa gtagattgct tgctactctt
      481 ttagcacctt taaaaagtga ctatgatctc attattattg atactgtacc aacgccaagc
      541 gtttatacaa ataatgcaat cgtggcaagt gattacgtta tgatcccttt acaagcagaa
      601 gaagaaagta caaacaacat tcaaaactat atttcctatt tgattgattt acaagaacag
      661 tttaaccctg gactagatat gatcggtttt gttccttatt tagttgatac ggacagcgca
      721 acgataaaat caaacctgga agaactgtac aaagaacata aagaggataa tttggttttc
      781 cgaaatatta tcaagcgaag taataaagta agtacctggt ctaaaaacgg cattacagaa
      841 cacaaaggct atgacaaaaa agttttatct atgtatgaga acgtattttt tgaaatgctt
      901 gagcgaat
//
LOCUS       DAXLPI010000277          902 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.286, whole genome shotgun sequence.
ACCESSION   DAXLPI010000277 DAXLPI010000000
VERSION     DAXLPI010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.286"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>902)
                     /locus_tag="ACF3T5_002849"
     CDS             complement(<1..>902)
                     /locus_tag="ACF3T5_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633338.1"
                     /translation="VLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNAS
                     EANIRSFFYYFYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFW
                     LAINIERSSFRKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSF
                     GVIDGNAIYENSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFH
                     ERSYLFYGNPDLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQL
                     FLDYYYVLDYYDLFLTNVKPIKIL"
BASE COUNT          327 a          149 c          112 g          314 t
ORIGIN      
        1 taaaatctta atcggtttaa catttgttaa aaataaatca taataatcta acacataata
       61 ataatccaaa aataattgtg cccagttttc taatttcttt gatacatcca aatccgcatt
      121 tgcttgtaat tcttttttga gtttttccat tatgtgataa gttcgaggtt cttcttcttt
      181 catttcttcg atatacgaac gattaaaaaa aagatctgga ttaccataaa atagataact
      241 tctttcatga aatgcaataa aattgaatat gagttctgga tcctttatat ctaaagaaaa
      301 acggaacatg ctttgtaaaa ctttctccaa attctcaact gctcgataag aacaagtatt
      361 ttgtcgctga tgtgcatatg catgactatt ttcatatatt gcatttccat caattactcc
      421 aaaagaataa attattcgat aaaggaaaca cagttcatcg ctgttaaaag aaagattgat
      481 ttgttttgac cacttcttca aaagattaaa atgcggatca tctgagataa ctgatttata
      541 ttcattatct actcgtactt ttctaaatga acttctttct atattaatgg ctaaccaaaa
      601 agaaaataca cgtttttgaa aaatagttaa cgtaatatca taaatcccct caaaagcaac
      661 tatatacttc tctatttcct tttgttcgat atgaaatggc cacttatcac tactttcacg
      721 tgtagaattc caatagaaat aataaaaaaa gctacgaatg tttgcctctg atgcattcaa
      781 ttctaactga aatggatgaa atgttatatg ataacgccta aaatatggaa ttaagagctt
      841 cgcatatctg aaaacagtag atacactagt aaattgctct ttagcaaaat cttttagaac
      901 ac
//
LOCUS       DAXLPI010000278          877 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.287, whole genome shotgun sequence.
ACCESSION   DAXLPI010000278 DAXLPI010000000
VERSION     DAXLPI010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.287"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..603
                     /locus_tag="ACF3T5_002850"
     CDS             <1..603
                     /locus_tag="ACF3T5_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFD3633339.1"
                     /translation="ITFIKSNSVIKGDTAIGITFSSFLALGVILIGVANSSTDLFHIL
                     FGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLITSFDPLMAKAMGMNVNFYHYLLM
                     VLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISAAVGASSSLLGLFI
                     GYNFNIAAGSSIVLTAASLFVISFLISPKQKFLRKKERSL"
     gene            600..>877
                     /locus_tag="ACF3T5_002851"
     CDS             600..>877
                     /locus_tag="ACF3T5_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFD3633340.1"
                     /translation="MMRKWKVVVGSLGILIALFILGACSTNSKDKVASNEKLKVVVTN
                     SILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFY"
BASE COUNT          292 a          111 c          166 g          308 t
ORIGIN      
        1 attactttta taaaaagcaa tagtgtaata aaaggagata cagcaattgg tattactttc
       61 agttcttttt tagcgctagg agttatcctt ataggagttg caaatagctc aacggattta
      121 tttcatatct tatttggaaa cattttagct gtacaagata ttgataagtg gattacgatt
      181 ggcgtttctg ttttagtgtt ggttatagta gttctatttt ttaaagaatt attaattact
      241 tcttttgatc cattaatggc aaaagcaatg gggatgaacg tcaattttta tcattatcta
      301 ctgatggttc tattaacttt agtagcagta acagctatgc aaagtgtggg gactatatta
      361 attgttgcaa tgttaatcac gccagcagct acagcatatt tatatgcaaa tagcttgaaa
      421 actatgatat ttatttctgc agcagtcggg gcaagctcat cattgcttgg attgtttatt
      481 ggttataatt ttaacatagc cgcaggatca agtattgttt taacagcggc atccttattt
      541 gtaattagtt ttctaatctc accaaaacaa aaatttttaa gaaagaagga gagatcgtta
      601 tgatgagaaa atggaaagta gtagtgggaa gtctggggat tttgattgct ctttttatat
      661 taggagcgtg ttcaacaaat agtaaagata aagtggcttc gaacgaaaaa ttaaaagtag
      721 tagttactaa ttcgatttta gcagatatta ctgaaaatat agcaaaggat aaaattgatt
      781 tacacagtat tgtacctatt gggaaagatc cccatgaata tgaacctttg cctgaagatg
      841 ttcaaaaaac ttcaaaagca gacttgattt tttataa
//
LOCUS       DAXLPI010000279          869 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.288, whole genome shotgun sequence.
ACCESSION   DAXLPI010000279 DAXLPI010000000
VERSION     DAXLPI010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.288"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>869
                     /locus_tag="ACF3T5_002852"
     CDS             <1..>869
                     /locus_tag="ACF3T5_002852"
                     /inference="COORDINATES: protein motif:HMM:NF021909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFD3633341.1"
                     /translation="NYAFKVPDSVNPKDYFYIDLSENANFYGVTEANSVTMPNLLAPD
                     NSIIATGKYDIDTNRIQYEFTDYVAVHDNVSGKISLPIFIDPEVVTNTSYQTITASIG
                     NSKKDLSLLVDYSKNVGPQYNANGAGNIFNVDLNNHNFSHTIYVNSLKQTLGNKSIEI
                     RNQRKEENGYNSEIIYDKTNTQIKVYKVKSNGRLTDSFSIATNDSLEDVTNQCYIDFY
                     DNKINISFPSNIDNETYVVQYTSPYTDKKDIATRVVMYGYSPYQLGNTSWYWDNYVKL
                     TDGNASGDGSVISP"
BASE COUNT          328 a          120 c          135 g          286 t
ORIGIN      
        1 aattatgcat tcaaagtccc tgactcagtt aatccaaaag attattttta tattgacttg
       61 agtgaaaatg ctaattttta tggagttaca gaagctaact ctgtcacaat gcctaatctc
      121 ttagctccag ataattcaat tatagcaact ggaaaatacg atattgatac taatcgtatt
      181 caatacgaat ttacggatta tgtagcagtg catgataatg ttagtggaaa aatttcttta
      241 ccgatcttta ttgatccaga agtagtaact aatacttctt atcaaactat tactgcgagt
      301 ataggcaata gtaaaaaaga tttgagtctg ttagtggatt attctaaaaa tgtaggacct
      361 caatataatg ctaatggtgc tggtaacata tttaatgtag acttgaataa ccataatttt
      421 tctcatacta tctatgtcaa ttctttaaaa caaactttag gaaataaaag tattgaaatt
      481 agaaatcaaa gaaaagaaga aaatggatat aattctgaaa ttatctacga taagacaaat
      541 actcaaataa aagtttataa agtaaaatca aacggaagac taaccgacag tttttctatt
      601 gctacaaatg attcactaga agacgtgact aatcaatgct atattgattt ttatgataat
      661 aaaattaata ttagttttcc gagcaatatt gataacgaaa cttatgtcgt tcaatatact
      721 tctccttata cagataaaaa agatattgct actagagtag ttatgtatgg ttactctcct
      781 tatcaattag gcaatactag ctggtactgg gataattatg taaaactaac agatggaaat
      841 gcaagtggtg acggtagtgt gatatcacc
//
LOCUS       DAXLPI010000280          863 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.289, whole genome shotgun sequence.
ACCESSION   DAXLPI010000280 DAXLPI010000000
VERSION     DAXLPI010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.289"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            46..195
                     /gene="rpmG"
                     /locus_tag="ACF3T5_002853"
     CDS             46..195
                     /gene="rpmG"
                     /locus_tag="ACF3T5_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFD3633342.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            219..>863
                     /locus_tag="ACF3T5_002854"
     CDS             219..>863
                     /locus_tag="ACF3T5_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFD3633343.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEEQVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSS"
BASE COUNT          321 a          138 c          158 g          246 t
ORIGIN      
        1 tcaagcatat cttgaagatc gataagtatt gaaggaggaa gatttatgcg tcaaaatatt
       61 attttggaat gtgtcgaaac tggagaacgt ctgtatctca ccagcaaaaa taaacggaat
      121 aaccctgaac ggttagaatt aaaaaagtat tcgccaaaat tgcgcagacg ggcaattttc
      181 aaagaagtga aatagctaag tggtaaaaag gtgacaaaat gggcatacca gttacaatcg
      241 tttctgggtt tttaggggct ggaaaaacaa cgctaatcaa tcaagccttg caagtctcac
      301 cctaccccag ggaagaaatt attataattg aaaatgagtt tggtgaagtt gggattgatc
      361 acaaattatt agttcatagc gaggaacagg tgttacaact aaataatgga tgtatgtgtt
      421 gcagtcttcg cagtgattta ttagcaatat tgatttcttt actggaaaca atagaagaac
      481 atcaacaacc aatttcacaa atcatcattg aaactacagg tatagcagat ccccaaccaa
      541 tcattcaaac acttttaaca gcgcctcaac taagaggaag agtatatatc gatagtttat
      601 tgactgttat tgatagtgac agttttgaac ttattgaaag agaaccacaa gcccttaagc
      661 aacttgtcat ggcagatcgc atttttgtgt ctagaaaaca aaacttgaat ttatctgcac
      721 taaaaaaatt acagagagaa atcaaaagta ttaacccttt atccgatatt cgaatgttca
      781 cacatagtga aacgattcaa aaaaaggatt tttataattt gaaaaaattt aatcatccct
      841 tagtttttga aagtgattca tca
//
LOCUS       DAXLPI010000281          847 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.290, whole genome shotgun sequence.
ACCESSION   DAXLPI010000281 DAXLPI010000000
VERSION     DAXLPI010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.290"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..106
                     /locus_tag="ACF3T5_002855"
     CDS             <1..106
                     /locus_tag="ACF3T5_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3633344.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="ACF3T5_002856"
     CDS             complement(359..508)
                     /locus_tag="ACF3T5_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633345.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT          290 a          132 c          124 g          301 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaa
//
LOCUS       DAXLPI010000282          847 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.291, whole genome shotgun sequence.
ACCESSION   DAXLPI010000282 DAXLPI010000000
VERSION     DAXLPI010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.291"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            69..644
                     /locus_tag="ACF3T5_002857"
     CDS             69..644
                     /locus_tag="ACF3T5_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFD3633346.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(713..>847)
                     /locus_tag="ACF3T5_002858"
     CDS             complement(713..>847)
                     /locus_tag="ACF3T5_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3633347.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          236 a          144 c          181 g          286 t
ORIGIN      
        1 cgttctttta aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg
       61 ctaacaaaat gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta
      121 ttgggatcaa ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg
      181 attctatcgg aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag
      241 gattgtccag cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc
      301 caatccaaat tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag
      361 ataaaaacag cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg
      421 tttcttcgat cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga
      481 tcgttcagct attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg
      541 cgggtacaga ctaccttgat cgcttgttgt ctatcgtcgt tgtttcatta gtcatcacta
      601 tgttgcccca acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat
      661 tagcaaaata atgctaatct ggctctgttt tttcatttta ttatttgtca aattaagcga
      721 gacaaaactt tccatttacg taactcaaaa aaacttctaa tggtgtttgg taatttagtg
      781 attttctagg gatatggttt cttttggacg caacggatga gatgaatcct tgattgactt
      841 ggttgaa
//
LOCUS       DAXLPI010000283          841 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.292, whole genome shotgun sequence.
ACCESSION   DAXLPI010000283 DAXLPI010000000
VERSION     DAXLPI010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.292"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            1..233
                     /locus_tag="ACF3T5_002859"
                     /pseudo
     CDS             1..233
                     /locus_tag="ACF3T5_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            282..365
                     /locus_tag="ACF3T5_002860"
     CDS             282..365
                     /locus_tag="ACF3T5_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFD3633348.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            490..>841
                     /locus_tag="ACF3T5_002861"
     CDS             490..>841
                     /locus_tag="ACF3T5_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFD3633349.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFES"
BASE COUNT          348 a          117 c          140 g          236 t
ORIGIN      
        1 ggaaatttag gcgcacaaaa agaaaaacga aatgatacac caatcagtgc aaaaaaagat
       61 ataatgggag ataagacggt tcgtgttcgt gctgacttgc accatatcat aaaaatcgaa
      121 acagcaaaga atggcggaaa cgtaaaagaa gttatggaaa taagacttag aagcaaactt
      181 aagagtgtgt tgatagtgca gtatcttaaa attttgtgta taataggaat tgaagttaaa
      241 ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta ttccaaatgc
      301 gtaatgtaga taaaacatct actgttttga aacagactaa aaacagtgat tacgcagata
      361 aataaatacg ttagattaat tcctaccagt gactaatctt atgacttttt aaacagataa
      421 ctaaaattac aaacaaatcg tttaacttct gtatttgttt atagatgtaa tcacttcagg
      481 agtgataaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
      541 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
      601 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
      661 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc
      721 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
      781 gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
      841 c
//
LOCUS       DAXLPI010000284          839 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.293, whole genome shotgun sequence.
ACCESSION   DAXLPI010000284 DAXLPI010000000
VERSION     DAXLPI010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.293"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..401
                     /locus_tag="ACF3T5_002862"
     CDS             <1..401
                     /locus_tag="ACF3T5_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFD3633350.1"
                     /translation="PTEPSKPTEPSEPTEPSTPTEPSTPTEPSTPTVPNKPVDTNPIE
                     NPVNTDTGVVIVAVEDSKPIIQLANGTTKKVEAKEIGANVQKDGTVTVKGSDGKMKVL
                     PKTGDTENIALSVLGSLMVLGSAFIFKKRI"
BASE COUNT          323 a          146 c          164 g          206 t
ORIGIN      
        1 aaccaacaga accaagcaaa ccaacagaac caagcgaacc aacagaacca agtacaccaa
       61 cggaaccaag tacaccaacg gaaccaagta caccaacagt accaaacaaa ccagtagaca
      121 caaacccaat cgaaaaccca gttaatacag atacaggcgt tgtgattgtt gcagttgaag
      181 atagtaaacc gattattcaa ttagcaaacg gaacaacaaa gaaagttgaa gctaaagaaa
      241 ttggtgcaaa cgttcaaaaa gacggaacag taacggttaa aggttcagac ggaaaaatga
      301 aagttttacc aaaaacagga gacacagaaa atattgcatt gtctgtccta gggtcattaa
      361 tggtattagg atcagcattc atttttaaaa agcgaatcta attaagtatt tataaaagcg
      421 tggtcgcaag tttcactagc agccatgctt ttattgaatc attctaggac gttttgaagg
      481 ggaatgtata ccttaaaatt aaacaagcgc ttagaatcgt tctaggcaac accaataaca
      541 tattgctagt aggtcggctt ttcttttcac ttgttgcgaa tacaagcgtg tgagttagaa
      601 agcaacttgc ttgccatcgt ctaatcatct aaccaatgat caactaatgt gattattaga
      661 accactaaaa gtggtgcgat aactaacgag ataggttctg ttgcaaagtt ttccaaaaaa
      721 tctattttag tgtaacattg agaaaaaaga cagagaggac aatatactgc aaaataatat
      781 taagtagtgg gggggactta ttgagtatgg ggattgtaat gaggcagtat aataatttt
//
LOCUS       DAXLPI010000285          834 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.294, whole genome shotgun sequence.
ACCESSION   DAXLPI010000285 DAXLPI010000000
VERSION     DAXLPI010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.294"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            3..581
                     /locus_tag="ACF3T5_002863"
     CDS             3..581
                     /locus_tag="ACF3T5_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFD3633351.1"
                     /translation="MDNKTNNTKTEIKKNDISKASEAIKGELVFEDKVIQKIIGIALD
                     EIDGLLTVDGGFFSNIAGKLVNTDNTTSGVDVEVGKKQVAVDLSIVAEYGKDVTTIYD
                     KIKQVISNEVRKMTGLDVIEINVNVVDVKTKEQYEKDSVTLQDHLTDAASATGEFASK
                     QIEKSKEALGVASEKVNDGVQNVKEATEPRVK"
BASE COUNT          335 a           95 c          151 g          253 t
ORIGIN      
        1 taatggataa taaaacaaac aatacaaaaa cagaaattaa aaagaatgat atatcaaaag
       61 cttctgaagc tattaaagga gaattggttt ttgaagataa agtaattcaa aaaataatcg
      121 gtattgcatt agatgaaatt gatgggcttt tgacagttga tggaggattc ttttcaaata
      181 tagccggaaa attagtaaat acagataaca caacttctgg agtcgatgtt gaagtaggta
      241 aaaaacaagt tgcagtagat ctttcaatag tggctgaata tggtaaagat gtaactacaa
      301 tttatgataa aattaagcaa gtaatctcaa atgaagttag gaaaatgact ggcttagatg
      361 taattgaaat taacgtaaac gtcgtagatg taaaaacaaa agaacaatac gaaaaagatt
      421 cagttaccct acaagatcat cttactgatg cagcttctgc tactggagaa tttgcttcaa
      481 aacaaattga gaaatcaaaa gaagctttag gagtagcaag tgaaaaagtt aatgatggtg
      541 tacaaaacgt aaaagaagca acagaacctc gtgtaaaata gtaacttcct ttctgtaata
      601 tacaacatat aaaattgaaa gactaaattt aaggctctta gatattttaa tgtagatata
      661 gaagaaagaa ctgaaatata ataaaaccag ttaggtatag gacttaactg gttttagttt
      721 ctttaagtgg gagatcaaca tctgaataag cattttgtga aagatgtaga aatctttata
      781 gctgttggtt actcattttg tatttcttat ttagttcttt tgtctctttc tagt
//
LOCUS       DAXLPI010000286          825 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.295, whole genome shotgun sequence.
ACCESSION   DAXLPI010000286 DAXLPI010000000
VERSION     DAXLPI010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.295"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..342
                     /locus_tag="ACF3T5_002864"
     CDS             <1..342
                     /locus_tag="ACF3T5_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633352.1"
                     /translation="RVYRRKLPYLINTPSTIWFLLMNKERIIGFSSIQISNEFIDIGD
                     VFVEDENDDTRKYLLEKVLFFIDKKCERKCVRISLKKDQYLNNLKHYCFKQYRETKNY
                     LFFRREINEQD"
     gene            329..>825
                     /locus_tag="ACF3T5_002865"
     CDS             329..>825
                     /locus_tag="ACF3T5_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFD3633353.1"
                     /translation="MNKIDMPVLNVKMVPVEKIKSNNYNPNHVAEPEMELLELSILKD
                     GYTQPIVCYYDDKKDEYIIIDGFHRYLIGKSKLDLKELPLVVIDKPLEQRMSSTIRHN
                     RARGKHEIDKMSELVLLLVNEGWDDHKIGKELGMEKEEVLRLKQVTGLKSAFLNHEFS
                     ESWDE"
BASE COUNT          330 a           84 c          155 g          256 t
ORIGIN      
        1 agagtatata gaagaaaatt accttattta ataaatacac ctagtactat ttggtttttg
       61 ttaatgaaca aggaaaggat aattggattt tcttccatcc aaatttccaa tgagtttatt
      121 gatattggag atgtttttgt tgaagatgaa aatgacgata ctcgaaagta tctattggag
      181 aaggttttat ttttcataga caagaaatgt gaaagaaagt gtgtacgaat ttctttaaaa
      241 aaagatcagt acttaaataa tctgaaacat tattgtttta aacaatatag agagacaaag
      301 aattatttat tttttaggag agaaatcaat gaacaagatt gatatgcctg ttttaaatgt
      361 aaaaatggtt ccagtagaga aaataaaaag taataattat aacccaaatc atgtcgctga
      421 acctgaaatg gagcttttag aattatcgat tttaaaagac ggatatacac aaccaattgt
      481 ttgctattat gatgataaaa aagacgaata tattattatt gatgggttcc ataggtattt
      541 aattggaaaa agcaaacttg atttaaaaga acttccttta gttgtaattg ataagccatt
      601 ggagcaaaga atgtcaagta caattagaca caatagagct agagggaaac atgaaataga
      661 taaaatgtcc gaattagttc tactgcttgt caatgaagga tgggacgatc ataaaatagg
      721 gaaagaacta ggaatggaaa aagaagaagt tcttagatta aaacaagtaa cagggttgaa
      781 aagtgccttt ttgaaccatg aatttagtga atcatgggat gaatt
//
LOCUS       DAXLPI010000287          821 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.296, whole genome shotgun sequence.
ACCESSION   DAXLPI010000287 DAXLPI010000000
VERSION     DAXLPI010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.296"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..734)
                     /locus_tag="ACF3T5_002866"
     CDS             complement(<1..734)
                     /locus_tag="ACF3T5_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFD3633354.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVR"
BASE COUNT          238 a          178 c          140 g          265 t
ORIGIN      
        1 ttgcgaacag ccttaactaa ccctttgtgt gcatcagaaa tgaccagttt tacaccgaac
       61 aaacctcgtt gaacgaacga ttgaaaaacg gtatcccaat tatcttccga ttcgccgtct
      121 tgaacatcaa aaccaagaat cgtacgtcgt ccatcagaat caatgccgat accaatatat
      181 acaccttttg agacaatacg atgattttcc cttactttca tatacagggc atcacacatg
      241 aaaaatggat attccgagcc ttctaggctc cggtttctcc actcaaaaac aaatggatcc
      301 aactgtttca tcagatttga aacgaatgat ttagaaacag tcgcaccatc cgttagcagt
      361 tcaactgttt tagttacttt gcgagtggag acaccagaaa tgaccatttc aatcatggca
      421 gcgatcaaag ctttttcgct tcgctgatat ctttcaaaga tagtaggact aaacttaccg
      481 tctctggttc gtggaacatg aagttctaac gtaccaattc gagttgtaaa cgaacgatcg
      541 tagtaaccat ttctttgtga ttgccgttct tctgaacgtt cataggcatt tgcctgaata
      601 tattgatttc gttgttcttc catcagctga ttaaacatga tagtcataag tgtttttgcg
      661 gtatcattcg caccgctttc gtttataatt gcttgaattt cttcgctttc aaaagtaaaa
      721 tgtacttgag tcattgatat cagtcctatt cgagtatgtt ttctgcaaaa aacattgtac
      781 tcctaactga gatcaatggc ttttttgatg cttcactttt a
//
LOCUS       DAXLPI010000288          793 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.299, whole genome shotgun sequence.
ACCESSION   DAXLPI010000288 DAXLPI010000000
VERSION     DAXLPI010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.299"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            42..>793
                     /locus_tag="ACF3T5_002867"
     CDS             42..>793
                     /locus_tag="ACF3T5_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633355.1"
                     /translation="MFTSCKEKRLKFAIRKKKGGGAASFIIGCLIFGNIFLNGAIANA
                     SSIENSYAISGFGNQTKGGYFLYNKGIKTESVIDNQVAFCFDKNVIAKLNDRGFSNPG
                     YQLYNEKDEQLLKVYSNEKINKVRNIMAYAWDNFITKYPYLNTYTAASNDDRASLARG
                     ISHAIWVTLGQTTEKKSRSDLGGVNTNAYKFYKEALNYKPLAKDINNYDISLLIPTDD
                     NNILQVNASQSLITVKKIDPDKPVNPDPETPVT"
BASE COUNT          295 a          106 c          144 g          248 t
ORIGIN      
        1 ataaaacatg aacgttcatt ttgcggtaag gagagatggg aatgtttact agttgtaaag
       61 aaaaaagatt gaaatttgca attagaaaga agaaaggtgg tggtgctgcg tcctttataa
      121 ttggctgttt aatttttgga aacatatttc tcaatggcgc tattgctaat gcgtctagta
      181 tagaaaatag ttatgcaata agtggttttg gtaatcagac aaaaggtggg tatttcttat
      241 ataataaggg tattaaaact gaaagtgtta ttgataacca agtagctttt tgttttgaca
      301 aaaacgttat agctaaactg aatgatcgtg ggttttcaaa ccctggttat caattataca
      361 atgaaaaaga cgaacaactc ttgaaagtat atagtaatga aaaaattaat aaagtaagaa
      421 atatcatggc ttacgcttgg gataatttta ttactaaata tccctattta aatacttata
      481 cagccgcttc taatgatgat cgtgcttctc ttgccagagg aattagtcat gctatttggg
      541 taaccttggg acaaacaaca gagaaaaaat caagaagtga tttaggtggc gtaaatacta
      601 atgcttataa attttataaa gaagctctaa attacaagcc acttgcaaaa gatattaaca
      661 attatgatat tagtttactt attccaactg acgacaataa tattttgcag gtaaatgctt
      721 ctcaatcatt aataacggtt aaaaaaatcg atccagataa accagtaaat ccagatccag
      781 aaactccggt aac
//
LOCUS       DAXLPI010000289          783 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.300, whole genome shotgun sequence.
ACCESSION   DAXLPI010000289 DAXLPI010000000
VERSION     DAXLPI010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.300"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..672
                     /locus_tag="ACF3T5_002868"
     CDS             <1..672
                     /locus_tag="ACF3T5_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFD3633356.1"
                     /translation="SGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDN
                     NKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTD
                     FLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRK
                     IIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
BASE COUNT          290 a          120 c          146 g          227 t
ORIGIN      
        1 agcggtaaat actatatttc tttgttgtgt aaagaagaaa ttatcgaatt acctaaaact
       61 aattccacaa ttggtattga cttaggtatt atggactttg cgattctttc tgatggacaa
      121 aaaattgata ataataaatt cacgtccaaa atggaaaaga aactaaagcg tgaacaacgc
      181 aaattgtcta ggcgtgcttt attggctaaa aataaaggta ccaatctatt tgaagctaaa
      241 aattatcaaa aacaaaaacg caaagtagct agattgcatg aaaaagtaat gaatcaacgt
      301 actgattttc tgaataagtt gagtacagaa atgatcaaaa atcacgatat tatctgtatt
      361 gaagacttaa acacaaaagg tatgttgcga aatcataaat tagctaaaag tatttctgat
      421 gtttcttggt caaagtttgt gactaaatta caatacaagg ctgactggta tggacgaaaa
      481 atcatcaaag tagataaatg gtttccgtct agtcagattt gttcagagtg cggacataaa
      541 gatggcaaga aatctcttga aattcgagaa tggacttgtc ctgtttgtca tactcatcat
      601 gatcgtgata tcaatgctag tatcaatatt ctgaccgaag gtctaagact acattctatg
      661 ggattggctt agatccaatc atcaagaacc gtaggaacta cggggatagc ttggtaaata
      721 agagacaccg ctgttagtaa agaaatacgc tatcaagtat gctctattcc caagaagctc
      781 cca
//
LOCUS       DAXLPI010000290          770 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.301, whole genome shotgun sequence.
ACCESSION   DAXLPI010000290 DAXLPI010000000
VERSION     DAXLPI010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.301"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            27..>770
                     /locus_tag="ACF3T5_002869"
     CDS             27..>770
                     /locus_tag="ACF3T5_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3633357.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPG"
BASE COUNT          269 a          134 c          145 g          222 t
ORIGIN      
        1 caaagaatta taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg
       61 taataataga gtcttatttc aaaatgaatc aatctgttgc taaaactgcc cattgcttga
      121 atcgttcaag acaaacgatc cataaagtat atctgttctt caagcaagga aaatcagcct
      181 tagaatatta tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt
      241 tacccgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg
      301 tgattgttgg tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt
      361 tcaaaaaggg gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg
      421 gacatcaaga aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg
      481 attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga
      541 aacataaaag tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga
      601 aaccgtgtgg tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg
      661 taccgaaaaa cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga
      721 aacagatcag taatgtcaat gatattgcca tttatttcgc tgatccagga
//
LOCUS       DAXLPI010000291          767 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.302, whole genome shotgun sequence.
ACCESSION   DAXLPI010000291 DAXLPI010000000
VERSION     DAXLPI010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.302"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..298
                     /locus_tag="ACF3T5_002870"
     CDS             <1..298
                     /locus_tag="ACF3T5_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633358.1"
                     /translation="EKKGDYDAYNYVLNLEKQTYYTLRKNDDNDFWTMLPKMLSIDSK
                     LSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGYKVDEQCPQSIIFYIE"
BASE COUNT          317 a           84 c          117 g          249 t
ORIGIN      
        1 tgaaaaaaag ggagattatg atgcatataa ttatgtatta aatctagaaa agcaaacata
       61 ttatacacta cgtaaaaacg atgataatga cttttggact atgttaccta aaatgctttc
      121 gattgactct aagctttccc ttttagagga atttattaat ttagacattg aagaattttg
      181 cgggggaaaa gagttaatta atatggtaga gagtgattat catacctatt ataaagaaag
      241 ttgtggatac aaagtggatg agcaatgtcc acaatcaatt attttttaca ttgagtaata
      301 tgaactatta agcaggaaag caactagaag ctactgattt agtatttaca aatataaact
      361 atattgctaa caattatttt ctagaaggaa taaaagaact aagaatcaag acgttatctt
      421 gattcttagt tcttttaaca tggaaaagat ggataatgca ctgttaattc atgatgcatc
      481 atcagaaaag ttggaaggag aagatagaaa tcattaaatg atttctacag atggtaaatg
      541 aaaaaacaaa atattagcaa aaatttgcct aaatagatag taacactttc taaattaaat
      601 acaagcgcat tcataaaatg tttatacaaa tatctatgtg ataaaaattg gatatgttca
      661 caataaatag gaattaaaat aacgaattta cgtttgtttt tatttaccaa tcaaattatc
      721 ttaatgcagg aataaaaaat taaattacat attgttaatc atttaca
//
LOCUS       DAXLPI010000292          762 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.303, whole genome shotgun sequence.
ACCESSION   DAXLPI010000292 DAXLPI010000000
VERSION     DAXLPI010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.303"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            251..>762
                     /locus_tag="ACF3T5_002871"
     CDS             251..>762
                     /locus_tag="ACF3T5_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFD3633359.1"
                     /translation="MVKKIDFHIHTVSSHKDYDFSYSPEWLKKYVRAAELDAIAITNH
                     DLFDESNFKQIQYDLPNTKVFPGIELSLEEGHVNIIFPEEDSPNLVHFSEWLSTQNLG
                     QNGKVSTEDFCTHMSNWENGIYIFELGKSNALSVPEKLESICSVGGVANQLKFQTFYR
                     IEDALTPVLF"
BASE COUNT          264 a          105 c          134 g          259 t
ORIGIN      
        1 tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt ctttatttaa
       61 aactttgcaa cagaaccatt tcgaagatcc ttaatttaaa taaattagag gaggatttat
      121 attgccgcaa acaagttcta aaatcaatgt cactgagtgg ttgtttgtat ggcggatatc
      181 aagcatatta gtcagcgttt acatgatata atcaagacat gagtttccaa attattatgg
      241 aggtctacat atggttaaaa aaattgattt tcatatccac acggtttcta gtcataaaga
      301 ttatgatttt tcttattctc cagagtggct aaagaaatat gttcgagcag ctgaattaga
      361 cgcaattgct attactaatc acgatttatt tgatgaaagt aattttaaac agatccagta
      421 tgatttgcca aatactaagg tttttccagg tatagaactg agtcttgaag agggacatgt
      481 aaatattatt tttccagaag aagatagtcc taatctagtt catttttctg aatggctatc
      541 tacccaaaat ttaggacaga acggaaaagt atctactgaa gatttttgta ctcacatgag
      601 taattgggaa aatggaattt atatctttga actaggaaaa agtaacgcct tatcagtacc
      661 agaaaaattg gaatctattt gttctgttgg tggagtagca aatcaattga aatttcaaac
      721 tttttataga atagaagatg ctttaacacc agtattattt ag
//
LOCUS       DAXLPI010000293          746 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.304, whole genome shotgun sequence.
ACCESSION   DAXLPI010000293 DAXLPI010000000
VERSION     DAXLPI010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.304"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>746
                     /locus_tag="ACF3T5_002872"
     CDS             <1..>746
                     /locus_tag="ACF3T5_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFD3633360.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKL
                     ALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLES
                     RFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT          251 a          134 c          135 g          226 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaatc catgaaaagc
      481 ttgcccttaa gggcattact atatccgttc cgccacgtaa aaacatggat aaatcagaaa
      541 aactagacca cagcttactt ggaaaacaaa gaaaaacagt tgagactgtt ttttcttcct
      601 tagaaaaatt aggttgtcaa aatttcaact cacgttctgt taaagggcta gagagtagat
      661 ttgaaagcat attactggct tacagtgttc tattaagtcg agcacaacga cgttttgaag
      721 gaactttgag atattcttta ggatat
//
LOCUS       DAXLPI010000294          733 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.306, whole genome shotgun sequence.
ACCESSION   DAXLPI010000294 DAXLPI010000000
VERSION     DAXLPI010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.306"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..619
                     /locus_tag="ACF3T5_002873"
     CDS             <1..619
                     /locus_tag="ACF3T5_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFD3633361.1"
                     /translation="PNGAGKSTLLKAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKA
                     DIDFTFPIKVKECVSMGTYAGMKVFQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSG
                     GQFQRVLLARCLAQNADFIFLDEPFVGIDLISERIIMDTLKELKNQGKTVLIVHHDLS
                     KVKKYFDNIILLNRNVIAHGSVESVFNEENLKKAYGDTIFIGERE"
     gene            621..>733
                     /locus_tag="ACF3T5_002874"
     CDS             621..>733
                     /locus_tag="ACF3T5_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFD3633362.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFII"
BASE COUNT          270 a           81 c          143 g          239 t
ORIGIN      
        1 tccaaatggt gctggaaaat ctacattact taaagcaatg ttaaatatta tccctcatca
       61 gggagaggtc tccattaata atgaagatat caataaaaaa ctggtaaaga ttgcatatgt
      121 agagcaaaaa gctgatattg attttacttt tccaataaaa gtaaaagaat gtgtatctat
      181 gggaacgtat gccggaatga aagtttttca aagaattaaa aatgcagaat ggcaaagagt
      241 ttcaaaggct ttggaaaagg tcgatatggg aaaatattct aatcatcaaa taggagaatt
      301 atctggagga caatttcaac gtgtactttt agcaagatgt cttgctcaaa atgcggattt
      361 catattttta gatgaaccat ttgtagggat agacttaatt agtgaacgaa taattatgga
      421 tacgttaaaa gaattgaaaa atcagggaaa aacagtgcta attgtgcatc atgatttaag
      481 caaggtaaag aagtattttg ataatattat tcttttgaat cgaaatgtaa ttgctcacgg
      541 aagtgtagaa tctgtattta atgaagagaa tttaaaaaaa gcctatggag atactatatt
      601 tattggagag agggaataac atgcttcaaa attttattaa tggtctgtat gactttcact
      661 ttttacagaa tgctctaatt acatctgtag ttatagggat tgtttctgga acagtaggtt
      721 gttttattat ttt
//
LOCUS       DAXLPI010000295          705 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.307, whole genome shotgun sequence.
ACCESSION   DAXLPI010000295 DAXLPI010000000
VERSION     DAXLPI010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.307"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..89
                     /locus_tag="ACF3T5_002875"
     CDS             <1..89
                     /locus_tag="ACF3T5_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFD3633363.1"
                     /translation="AKVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(352..>705)
                     /locus_tag="ACF3T5_002876"
     CDS             complement(352..>705)
                     /locus_tag="ACF3T5_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HFD3633364.1"
                     /translation="RATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSS
                     LETPSISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQ
                     PPTPPIPKTPKLPGI"
BASE COUNT          200 a          106 c          127 g          272 t
ORIGIN      
        1 acgccaaagt aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata
       61 gttatctatt atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag
      121 ttacacgtta ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta
      181 ccaattaatt cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc
      241 ttatcgatac aaattcctcg taggcgctcg ggaccccaac atgaggaaat gtttttataa
      301 aaaaagagta gttaccgttt tttggtaact actcttttcc ttttttaagg tttaaatacc
      361 tggaagttta ggtgttttgg gtattggtgg tgtaggtggc tgcaagcttt ccaatttttg
      421 ttgaattgct ttaaattcag gtgtctcttg gtgtttattt agaaccattt tttgctctat
      481 tcgttctaaa gtcggttgaa tttctttccc tgatacttta cgattcaatt ctttttctaa
      541 ggtttctttt ggactaatag aaggtgtttc caaacttgaa tagagtttta ctccttctct
      601 gttatatagc cttatatcgc taaataagcc cgttttatga agggtttcta aattggtcgg
      661 taagtttttg acaacaatat catgcgcttg ttttggtgtt gccct
//
LOCUS       DAXLPI010000296          699 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.308, whole genome shotgun sequence.
ACCESSION   DAXLPI010000296 DAXLPI010000000
VERSION     DAXLPI010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.308"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..151
                     /locus_tag="ACF3T5_002877"
     CDS             <1..151
                     /locus_tag="ACF3T5_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFD3633365.1"
                     /translation="DIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMDQALEEYIRKY
                     LPDKL"
     gene            169..441
                     /locus_tag="ACF3T5_002878"
     CDS             169..441
                     /locus_tag="ACF3T5_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFD3633366.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            443..>699
                     /locus_tag="ACF3T5_002879"
     CDS             443..>699
                     /locus_tag="ACF3T5_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFD3633367.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEK"
BASE COUNT          272 a           92 c          133 g          202 t
ORIGIN      
        1 agatataatg ggagataaga cggttcgtgt tcgtgctgac ttgcaccata tcataaaaat
       61 cgaaacagca aagaatggcg gaaacgtaaa agaagttatg gatcaagcct tagaagaata
      121 tattcggaaa tatttacctg ataaacttta aaaaggagtg aaaatgaaat ggcagttacg
      181 tatgaaaaaa cttttgaaat agagatcatt aacgaattat cggcaagcgt ttataatcga
      241 gtattaaact atgttttgaa tcatgaatta aataaaaatg actctcaatt attggaagtc
      301 aatttattaa accaattaaa gcttgcaaaa cgtgtaaatc tttttgatta ttctttagaa
      361 gaattacaag ccgttcatga gtattggcgg tcaatgaatc gttactcaaa acaagttttg
      421 aataaagaga aagtggctta atatggcaaa tatagtcaat tttactgaca aacaatttga
      481 gaatcgctta aatgataatt tagaagaatt gattcaagga aaaaaagcgg ttgaatcgcc
      541 aaccgctttt ttacttggtg ggcaaccagg gtcagggaaa accagtttgc gatcagcaat
      601 ttttgaagaa acacaaggga atgttattgt cattgataat gataccttta aacaacagca
      661 ccctaatttt gatgaactag tgaaacttta tgaaaaaga
//
LOCUS       DAXLPI010000297          698 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.309, whole genome shotgun sequence.
ACCESSION   DAXLPI010000297 DAXLPI010000000
VERSION     DAXLPI010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.309"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>698
                     /locus_tag="ACF3T5_002880"
     CDS             <1..>698
                     /locus_tag="ACF3T5_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFD3633368.1"
                     /translation="MYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAIYMDATYI
                     PLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLF
                     ITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKK
                     AALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIK
                     KYSHRKEQFQNEES"
BASE COUNT          214 a          151 c          152 g          181 t
ORIGIN      
        1 aaatgtatgg gcatcactac acgccccaaa ccatgtccaa tatgactaaa tcatttacgg
       61 aagaagtcac ttcatttaag gaacgtgagc ttcatgaccg ttatgccgct atttatatgg
      121 acgcaaccta tatcccttta aagcggaaaa ccgtcgccaa ggaagccatt catatcgcag
      181 ttggcattcg cccggacggt tcaaaggaag tgttgagcta tgcgattgca ccaactgaat
      241 ctatcacgat ttgggaagag attttaatag acctccagga acgtggcgtg aaaaacgtcc
      301 tccttttcat cacggatggc ttaaagggga tggcaggggc cgtgcagcgg ttctacccca
      361 aagcccgttt tcaacattgc tgtgtgcacg tttcccgtaa tatcagtcac aaagtgcgtg
      421 tcgatgatcg taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcg tcatctaaaa
      481 aggcggcatt ggaagcacgt ggtgcttttg cggaaaaatg gaaaaccagt tatccaaaaa
      541 tggtggaatc gatcctttcg aacgattacc tgctcacttt ctatgatttt ccattagcca
      601 tacgcaagac tatttactct acgaacttga ttgaatcctt taataagcaa atcaagaaat
      661 acagccacag aaaagagcag tttcaaaatg aagaatca
//
LOCUS       DAXLPI010000298          682 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.310, whole genome shotgun sequence.
ACCESSION   DAXLPI010000298 DAXLPI010000000
VERSION     DAXLPI010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.310"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            257..655
                     /gene="spxA"
                     /locus_tag="ACF3T5_002881"
     CDS             257..655
                     /gene="spxA"
                     /locus_tag="ACF3T5_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFD3633369.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          241 a          100 c          128 g          213 t
ORIGIN      
        1 gtacagtttt atacgctgtt tcatatgttt agaaaatttg aattagattt gaaaactcta
       61 atttttaaat gataaaaaaa gcgagaatgc tggttttact gtatattaac cttagataac
      121 tttcattttt ttaaaaaaat tatgaaaaaa aggtagctat ttaaaagttg attatgtata
      181 atagaaacag atgggagatt attatgatct tctgttacaa aaaagtgatt cttatcacga
      241 aaaggagtgg tgcacgttgt taacattata tacttcccca agttgtactt cctgcagaaa
      301 ggctcgtgcg tggttgcaag aacatcagat tccatttgtt gaacgtaata ttttttcaga
      361 accattgaac agttctgaat taaaagcgat tttacaaatg acagaagacg gaacagaaga
      421 aatcatctcg acacgttcca aagtattcca aaaactgaac atggatttag atgatctgcc
      481 gttacaagaa ttattggaac ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat
      541 gattgatgat aaacgtctgc aagttggttt taatgaagat gaaattcgtc ggttcctacc
      601 gcgcgatgta cgacaattag aattacgtca agcacaattg atggctggat tatagaagga
      661 taatacgaaa cttggaaaag ac
//
LOCUS       DAXLPI010000299          681 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.311, whole genome shotgun sequence.
ACCESSION   DAXLPI010000299 DAXLPI010000000
VERSION     DAXLPI010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.311"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          274 a           71 c          126 g          210 t
ORIGIN      
        1 agttaattat ttgaatgata tataaatttt aatcaaagaa agacaattta ttgttgaaat
       61 agttgttgaa gctttcgagg cgttcttgag gtacagttga aagctttata tccactttag
      121 aggtatacaa aagtttaata tgagaattct cagaagataa tctactataa aacagtagat
      181 aagtcagcgc ccaaaactaa ccattcgttg taaaagcacc gtcgtcgatg atcgcttaga
      241 ttggctatac gaaagtgagc aacaaaaaaa gtttgggaat aaaaagaaag cgtactgtaa
      301 tcttccaaaa ggttaagatg attttgataa aaaaatttaa tatatttgtt atttttataa
      361 tgtgatgata taaaatggat cgaacagttg aatgaggtgt ttatatgaaa aaaagtcact
      421 ccttatcgtc caaaacctaa aaacgttttt gaaagaggaa gcataaatat tttgaaggag
      481 aaaaggggca agagttaaaa gagaagaatc aaaaaagaag tccaagaaag aaaaaatgtg
      541 agtatatttt ttagaaagaa aggaattgtt gcgaaactag accgtaaaga tttataagta
      601 atcagtaacc catacttttt atatttaaag aggttatttt tctgtaaatc gttgtaaaaa
      661 atatctgagt tgtagtattt t
//
LOCUS       DAXLPI010000300          671 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.312, whole genome shotgun sequence.
ACCESSION   DAXLPI010000300 DAXLPI010000000
VERSION     DAXLPI010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.312"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>671
                     /gene="aac(6')-I"
                     /locus_tag="ACF3T5_002882"
     CDS             <1..>671
                     /gene="aac(6')-I"
                     /locus_tag="ACF3T5_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HFD3633370.1"
                     /translation="LTDERVLEFYDGRDKKYTLESLKKHYTEPWEDEVFRVIIEYNNV
                     PIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKK
                     ERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNAT
                     NVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTDKKKGYAKEKA
                     IYNFL"
BASE COUNT          282 a           54 c          120 g          215 t
ORIGIN      
        1 ggttaactga tgaaagagta ttagaatttt atgatggtag agataaaaaa tatacattag
       61 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
      121 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      181 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      241 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      301 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      361 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      421 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      481 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      541 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      601 tttttaaaac aaaatttagt actgataaga aaaaaggtta tgcaaaagaa aaagcaatat
      661 ataatttttt a
//
LOCUS       DAXLPI010000301          666 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.313, whole genome shotgun sequence.
ACCESSION   DAXLPI010000301 DAXLPI010000000
VERSION     DAXLPI010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.313"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>666
                     /locus_tag="ACF3T5_002883"
     CDS             <1..>666
                     /locus_tag="ACF3T5_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HFD3633371.1"
                     /translation="MYGYHYTPQTMSKMTKVLTEEVNAFKVRTLNDKYVAIFMDATYI
                     PLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRGVHDVLLF
                     ITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAVYQASSKE
                     EANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIESFNKQIK
                     KYS"
BASE COUNT          227 a          123 c          125 g          191 t
ORIGIN      
        1 aaatgtatgg gtatcactac acgccccaaa ccatgtccaa aatgactaaa gtgctgactg
       61 aagaagtaaa tgcatttaaa gttagaactc taaatgataa gtatgtcgct attttcatgg
      121 atgctactta tattccttta aaacggcaaa ccgtctccaa agaagcaatc tatattgcca
      181 ttggtatacg agaagacggc actaaagaag tactaagtta tgcgattgct ccgactgaat
      241 cgacctacgt ttggaatgag ttgttacagg acatttactc cagaggggtt catgatgtct
      301 tactgtttat caccgatggc ttaaaaggca tgaaagatac gattcaccag atttatccta
      361 aagctaaata ccaacattgc tgtgttcacg tttcccgtaa tattgctcat aaagtacgtg
      421 tcaaagatcg aaaagaaatc tgtgatgatt ttaaggctgt ttatcaagct agctcaaagg
      481 aagaggcaaa taccttttta gggagtatga ttgagaagtg gcagaaaact tatcctaaag
      541 tgacgcagtc actgataaaa aatcaagatt tattgacttt ttatgagttt ccgcctggta
      601 tccgccgaag catttactca actaatttaa tcgaatcttt caataaacaa atcaagaaat
      661 acagcc
//
LOCUS       DAXLPI010000302          658 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.314, whole genome shotgun sequence.
ACCESSION   DAXLPI010000302 DAXLPI010000000
VERSION     DAXLPI010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.314"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(375..461)
                     /locus_tag="ACF3T5_002884"
                     /pseudo
     CDS             complement(375..461)
                     /locus_tag="ACF3T5_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          234 a          113 c           94 g          217 t
ORIGIN      
        1 agaaaaatca atacataaat tatcatatct gtatatgtca tttaggtatt tcagttatgt
       61 tataatagtt ttgctgtctg ctaccccggc ctaatctggc aaggggagga ggagatataa
      121 tgaaatacct ttttgatttt gtttttgcaa tcaagccact tgcagatgta gtgtgcagtt
      181 acctctgcaa atggcttgat tgcaaaattg ccaaaaaaga cagtaagcct acccgttaat
      241 tctataatat agaaacgtaa aaaaaacccc atcagcctgc acgctgatgg ggtttggtga
      301 tataatgaaa tacctctttg ttactcataa ttataccata attagtcacg aatgcaacca
      361 atagaaaaaa ctacttagaa cggtagatca tcgctaataa caatgcattg tccaccaaat
      421 ggattttgtg aagcattaaa tacattgtta ttaattgtcg ctgcttttaa ctaggcctct
      481 ccccttctat tataaaagaa gatatatatc atttcttaat tacctactac cttaaaccaa
      541 aatttaagta ctacctaatt taagtagtac ttaaacaatc taaaaaatag ttatcccaat
      601 tgttatgctt ttataatacg attatttgta acaaaatgaa tattatataa ttaaacac
//
LOCUS       DAXLPI010000303          640 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.316, whole genome shotgun sequence.
ACCESSION   DAXLPI010000303 DAXLPI010000000
VERSION     DAXLPI010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.316"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..283
                     /locus_tag="ACF3T5_002885"
     CDS             <1..283
                     /locus_tag="ACF3T5_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFD3633372.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          214 t
ORIGIN      
        1 tttaatacaa gatgatttac accatacgca tcggttatgg ttaatgaata aaattaatct
       61 gtattttggg cattcttatg tttttgcttt ttatgattac aggacaaata taactgaagt
      121 agatttggtt atttctaatt actacatcga cactggaaaa acgccattac tgttaatgaa
      181 aaatatacct accgaaagaa actggcgttt gttcgagaaa acgatttatc aattgaaaaa
      241 agaaaaagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca aacggaaaaa
      301 tagtttttca tattttgccg tttttgagct aataccgagg gacagctgat tgaacaccat
      361 ttattgagag attgtgaaaa aactgtcctg catcaagtaa taagaacagc caagtaaaaa
      421 cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttggca agagaacacc
      481 gtttattcgg aaaaagggac tgtgacacta catgtcacag tccctttttg attgaattat
      541 ctcatttgtt ttctaatttt ttctttattt gagaaaatag ttgaactgga taaaaagtac
      601 tggacgtgtc aaaattattt tggatcaaat tagatctatt
//
LOCUS       DAXLPI010000304          628 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.317, whole genome shotgun sequence.
ACCESSION   DAXLPI010000304 DAXLPI010000000
VERSION     DAXLPI010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.317"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            30..497
                     /locus_tag="ACF3T5_002886"
     CDS             30..497
                     /locus_tag="ACF3T5_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFD3633373.1"
                     /translation="MKFCETKKVLNQLVADLSVFSVRIHQVHWYMRGERFLFLHPKMD
                     DLMEQINDQLDVISERLITLDGSPYSTLEEFFTNSKLEEEKGSWNKTIDEQIDYLLEG
                     YNYLVSTYEEGIETAGKEGDDCTEDIFIGSKSELEKEIWMLKAELGNSPELDK"
BASE COUNT          234 a           67 c          125 g          202 t
ORIGIN      
        1 ttaaaaaaat ttaaattagg agtgatatta tgaaattttg tgaaacaaaa aaagtattaa
       61 atcaattggt agccgattta agcgtatttt cagtaagaat acatcaagtt cattggtata
      121 tgcgtggaga aaggtttcta tttctacatc ctaaaatgga tgatctcatg gagcaaatta
      181 atgatcagtt agatgtaatt tcagaacgac tgattacact tgatggatct ccgtattcaa
      241 ctctagaaga gttttttaca aattccaaat tagaagaaga aaaaggctct tggaataaaa
      301 ctattgatga acaaattgat tatttattgg aaggttataa ttatcttgtt tcgacatatg
      361 aagaggggat tgaaactgca ggaaaggaag gcgatgactg tacagaggat atttttattg
      421 gttctaaatc agagctagaa aaagagattt ggatgttaaa agcagagtta ggtaactcac
      481 ctgagttaga taaatagata cagaacttca agtgaatggt tatgatattt ccttgtgaat
      541 agggagtgtt tttcagtgtt tctatgaata ctataagtga gattgataaa aaaacaaaaa
      601 catctaagga gagatttatt tctacctt
//
LOCUS       DAXLPI010000305          620 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.318, whole genome shotgun sequence.
ACCESSION   DAXLPI010000305 DAXLPI010000000
VERSION     DAXLPI010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.318"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..329
                     /locus_tag="ACF3T5_002887"
     CDS             <1..329
                     /locus_tag="ACF3T5_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFD3633374.1"
                     /translation="VTSEGCFKYFSKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRT
                     TKVPSLFVESSVDDRPMKTVSKDTNIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKI
                     AEGLSK"
     gene            344..577
                     /locus_tag="ACF3T5_002888"
     CDS             344..577
                     /locus_tag="ACF3T5_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFD3633375.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
BASE COUNT          246 a           78 c          118 g          178 t
ORIGIN      
        1 ttgttacaag tgaaggatgc tttaaatatt tctcgaaagc gtataatgtg ccttctgctt
       61 acatttggga aatcaacact gaagaagaag gaacaccaga tcaaataaaa cacttagttg
      121 aaaaattacg cacaacaaaa gttccctcct tattcgtaga aagcagtgtg gatgatagac
      181 caatgaaaac agtatcaaaa gataccaata ttcctatcta ttcaacgatt tttactgatt
      241 caattgcaga aaaaggacaa gatggtgata gttactatgc gatgatgaaa tggaacctgg
      301 ataaaattgc tgaaggcctt tcgaaataat ttggagggta ggtatggtac aaattaaaac
      361 gactactata ctaatttatt tactagcaat tcaggaatta tcaaaaagaa aaaagaaatt
      421 aaaaaataat gatttagcaa aagttctaag tgttagtcca gcatctgtaa gtgagatgct
      481 ggctaaactt agatatgagg actatttaga tataggattt caattaactg ataaagggaa
      541 aaaatttatt gacgattaca aaaaaggttt taagtaaaag actttatttt tagggaggat
      601 aagaaaatgg ctattggagt
//
LOCUS       DAXLPI010000306          619 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.319, whole genome shotgun sequence.
ACCESSION   DAXLPI010000306 DAXLPI010000000
VERSION     DAXLPI010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.319"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            290..>619
                     /locus_tag="ACF3T5_002889"
     CDS             290..>619
                     /locus_tag="ACF3T5_002889"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFD3633376.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYE"
BASE COUNT          229 a           70 c          101 g          219 t
ORIGIN      
        1 tttaaatctt ttgctaagtt gaactaatag agtcttttat gaatatcgaa gttagttttt
       61 cacctaactt cgttttttat agttggttat tataaaatag taatatagct gatttggatt
      121 caattcatat ttatttataa atcaaaaata cagagaatag tatataaatg tcctttccta
      181 ttcttaaaat gatgtttgta cttacttaaa ttaggtataa ttagtatatg ttttgtgaga
      241 aatttagact tacaataagc agtgaattta taatcaacgt ataggagaca tggatgaaat
      301 gaaactaatt ggtatagatt tagacggtac tcttttgaat tctgaccaga gcattagtca
      361 aaaaaatgca agaacaatga aacatttacc ggaagactgt tttccattca tttgttctgg
      421 aagagaagta gaagacatta ataatattct caaaaaatct ggtttaagga taccagcagt
      481 agggttaaat ggttctgttg gatttgatgg agaaaaaaag atatttgagt cttccttcga
      541 taaaaatgat gtaaaaaaaa ttgattctgt cctatcgaaa tttccaacca aaatatatac
      601 aaatctcgga agttatgaa
//
LOCUS       DAXLPI010000307          615 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.320, whole genome shotgun sequence.
ACCESSION   DAXLPI010000307 DAXLPI010000000
VERSION     DAXLPI010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.320"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(1..270)
                     /gene="rpsN"
                     /locus_tag="ACF3T5_002890"
     CDS             complement(1..270)
                     /gene="rpsN"
                     /locus_tag="ACF3T5_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFD3633377.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAVGDHTDLAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT          192 a          110 c           84 g          229 t
ORIGIN      
        1 ttaccaactt gcttttttta ctccaggaat caaaccttga tgggcaagct ctctaaactt
       61 aatacgggac ataccaaact tccgcatata ccctcttgga cggccatccg tctgatcacg
      121 aagcctcaga cgatttggat ttgaatctct tggcaattta gcaaggtcgg tgtgatctcc
      181 tacggctttt aattcttgac gtttgtctgc atatttttca atcattgcca tttgtttctt
      241 tgctttagca attttagact tttttgccat atacatttat actccttctg ttttgattta
      301 gacgtcacaa atcgtaaacg ttacgttttg acattgacac tttaccaaca aaaatttctc
      361 ttgtcaacaa taaataacat gaaataaaaa tcatatgaat agatatccat ttgaaaatga
      421 ttttatcgat acatagagat aatcagatta attaaaagaa cttgtagttt aagtgcaaaa
      481 aataatggtt catttttatt agtccattaa aacataaata ccgttttaag tagcttttct
      541 ctacttaaaa cggcattatg aaagaaacta tagttttatt caatctttta tgtttaactc
      601 gtttattaat gattt
//
LOCUS       DAXLPI010000308          604 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.321, whole genome shotgun sequence.
ACCESSION   DAXLPI010000308 DAXLPI010000000
VERSION     DAXLPI010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.321"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            51..>604
                     /locus_tag="ACF3T5_002891"
     CDS             51..>604
                     /locus_tag="ACF3T5_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HFD3633378.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRT"
BASE COUNT          221 a           97 c          119 g          167 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcac
//
LOCUS       DAXLPI010000309          600 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.322, whole genome shotgun sequence.
ACCESSION   DAXLPI010000309 DAXLPI010000000
VERSION     DAXLPI010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.322"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          257 a           71 c           62 g          210 t
ORIGIN      
        1 cattactagt aatatatact accttatttc agtagtatat attatattat tcttataaga
       61 caaggcttta ggctagaaaa caatagatta tatactacta aaaaatacaa taagtactac
      121 caatgtcaga tatatataat aattatgtac taccattaaa attaacttat ttaataagat
      181 actactgtgt taatctgaaa aaaattactt taaagtagtt acatactacc tgttaattta
      241 gatactacca ttaatactta aattagatac tacctatatt tatattaagt actaccgttc
      301 taagaataag ataataaaaa aatactacca aattgcaatt aaatactact taaaatgtaa
      361 aaaataagaa ctacccaaat taggtagttc ttaataagag aatatcagat aaatttgtac
      421 aagtcataaa aaaattaatc ttaaattttt aaatatatat acttgctaat tataaactac
      481 tttatttagg tagtacataa ttaacagata aaactacatg cttgtagttt gtctagctaa
      541 tgagttgcat atgatttata taaaggtgtg cagcataact tagttgaagt taagagaata
//
LOCUS       DAXLPI010000310          594 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.323, whole genome shotgun sequence.
ACCESSION   DAXLPI010000310 DAXLPI010000000
VERSION     DAXLPI010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.323"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            419..>594
                     /locus_tag="ACF3T5_002892"
     CDS             419..>594
                     /locus_tag="ACF3T5_002892"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3633379.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEA"
BASE COUNT          187 a          120 c          114 g          173 t
ORIGIN      
        1 cgcatcgacg aacgtcttcg tagtcagatt taacgttaaa atatcgctac tttgataagc
       61 ttttgccgct ttgtctgtga cgacaaaaga tctgcctgtt tgaatttctg tcacaaaaac
      121 gagattgtct ctgctttttt caacaacaac tccaatcgtt gaatgaacca tttctgcttc
      181 ctcccttctc tttcttttga tataatgtag tcgcaaatcg caccactcaa tgagttggcg
      241 gaaaacaatt cattgaaaaa aagagtcctg gacaatcacg ttcaggactc ttttacttct
      301 tttattcact taacaactaa cactctgtga gtgaaatgga ctcttcaatg tgatgttagg
      361 atgatttcat ggacacgatt tggtagaatc taactactaa gaaaaggacg tgtccacgat
      421 ggcaaaagcc aagaaaaaat ttgatgaaga ctttaagaag atgatcttag atttaaacca
      481 gtctggccaa tcggtcgaag aactggcagc gcaatatggt atcgctacac aaacgattta
      541 tcgttggaag aagcttcata caaagaatgt agcaaccggc atgactgagg caga
//
LOCUS       DAXLPI010000311          582 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.324, whole genome shotgun sequence.
ACCESSION   DAXLPI010000311 DAXLPI010000000
VERSION     DAXLPI010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.324"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            419..565
                     /locus_tag="ACF3T5_002893"
                     /pseudo
     CDS             419..565
                     /locus_tag="ACF3T5_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT          193 a           84 c           95 g          210 t
ORIGIN      
        1 aaaaaaaaga taaaacttga ttaattgaag tattgcttaa atttggaagc tatgagggaa
       61 gaataaaaga gttaggacgg gattatctac tatttttctt tgttttaaat atttgttatc
      121 tttgaacata aaagagaaat gtgtcgactg cagtgttgtg tccctcatca cgaataaacg
      181 gtgttcaatt agctgatctt gtattaactt aaaaaatggt aaagtacgag ttgctattcc
      241 ccattttcaa ttttctaacg ctcaggacaa aatactgttt tcacaacctc attggaataa
      301 aagcatatat attgtttcaa gaacgaaatc ttgttatctt tttaaagggc atctgtccaa
      361 aatctaattt taggaggtac gttttggtat ttattcgtac aaataaaaga gaattatctt
      421 tattcatgta acgcatccaa ttgatttttt catgtacgct cctggaacga ttttactgac
      481 gattgcttat ttaactgcga atcgctcatt agcttttatc atagctatcc atattttgaa
      541 taacgttctt ggctttgtat tatagataca gatgacaaaa aa
//
LOCUS       DAXLPI010000312          578 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.325, whole genome shotgun sequence.
ACCESSION   DAXLPI010000312 DAXLPI010000000
VERSION     DAXLPI010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.325"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>578)
                     /locus_tag="ACF3T5_002894"
     CDS             complement(<1..>578)
                     /locus_tag="ACF3T5_002894"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFD3633380.1"
                     /translation="LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGL
                     DYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCL
                     CHNDFSCSHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRM
                     YGNIDIEKAKEYQDIVEEYYPIETIVYGIKNI"
BASE COUNT          198 a          101 c           41 g          238 t
ORIGIN      
        1 ttaatatttt taattccata aacaatagtt tcaataggat aatattcttc aactatatct
       61 tgatattctt ttgctttctc aatatctata tttccataca ttcttaatat atcttctcca
      121 aaatttgttc ctatttcttc ttcactatct tcaagtaagt atataaaatc acaatattca
      181 tctataattc cagaatctcc aaaatcaatt attccagtta atctattatt gccatctaac
      241 aatagatgac tacaactaaa atcattatgg cataaacact ttttaccctc aaaaactgtt
      301 gttgcattta gtctttccat aaaactttct atataatctt tttctatatc agttaaatca
      361 ttataaatag tttcacgcaa caatatatac tcttctaata cattttgttt attatcaata
      421 gtacattcac taatatctgt ataatctaaa ccgtgcattt gtcttaaaaa actggcaata
      481 tctcgtttta acaaattttg ttcttcttct gacatagtag aataaatttc tggtgttaaa
      541 aaagttcctt taatttcttt ataacctagt atagataa
//
LOCUS       DAXLPI010000313          572 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.326, whole genome shotgun sequence.
ACCESSION   DAXLPI010000313 DAXLPI010000000
VERSION     DAXLPI010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.326"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>572
                     /locus_tag="ACF3T5_002895"
     CDS             <1..>572
                     /locus_tag="ACF3T5_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3633381.1"
                     /translation="APYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLK
                     GNSHSQRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNL
                     RQGYQLYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYS
                     YSNGPLECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          191 a          119 c          109 g          153 t
ORIGIN      
        1 atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc
       61 gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca
      121 cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct
      181 attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg
      241 ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc
      301 ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag
      361 atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata
      421 caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca
      481 gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg
      541 cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DAXLPI010000314          562 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.327, whole genome shotgun sequence.
ACCESSION   DAXLPI010000314 DAXLPI010000000
VERSION     DAXLPI010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.327"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(107..406)
                     /locus_tag="ACF3T5_002896"
     CDS             complement(107..406)
                     /locus_tag="ACF3T5_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFD3633382.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          178 a          106 c           64 g          214 t
ORIGIN      
        1 tctttattta gaataaaaaa atacagactc tgttcaaata aatgaaccta gcctgcatta
       61 tgagattgac tatacttttt aattcattga gatgaataac tcgtttttat aaatgcccaa
      121 aagtaatcgg tgccgaagcc attcctcgac ctaattcttt ttcaaatttt cctattgttg
      181 atatctccat ataaaacggt gatactggtt tagacaattc tcccctgatt gctaacggac
      241 gtaaactacg ttccaaatta tttatgactt taggataata ctgacctttt tctaagcctg
      301 tctttgcgtc tatcaaaagt atacgttcct tttctttgat ctctggattt aaaacgagat
      361 catataattt tagtaccatg ttttctattt cttcactttt agacatcacg atttcctccc
      421 aaaaatatat ttcttcccac tacattctat aaacaaagta tacgctacat ctagattttt
      481 ttaaatcatt actggattgt taaaaccttt caatacactg ttatggcaac gatttttatt
      541 ttaaaaacat tctaaattaa at
//
LOCUS       DAXLPI010000315          548 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.328, whole genome shotgun sequence.
ACCESSION   DAXLPI010000315 DAXLPI010000000
VERSION     DAXLPI010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.328"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..399
                     /locus_tag="ACF3T5_002897"
     CDS             <1..399
                     /locus_tag="ACF3T5_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3633383.1"
                     /translation="FDKLPKKVSSDVKDELKSIFKASELELTRERKEHFLEKYGCDSK
                     LSAACDILENGFEDAIQILSFPENIRRRIRTTNVLERLNEEIRRRERVIRIFPNINSI
                     TRIIGTLLMEKDTEWLASPRKYLEFNSNNI"
BASE COUNT          171 a           99 c          118 g          160 t
ORIGIN      
        1 tttgataagc tgccaaagaa agtttcttct gatgtaaaag acgagttgaa gagtatcttt
       61 aaagcctctg aacttgaatt gacacgtgaa agaaaagagc acttcttgga aaaatatggt
      121 tgtgattcaa aattaagtgc tgcttgcgac attttagaaa acggctttga agatgctatt
      181 caaattttgt cttttcctga aaatatccgc cgaaggattc gtacaaccaa tgtgttggaa
      241 cggttgaacg aagagattcg ccggagagaa cgagtgattc ggatttttcc aaatatcaat
      301 tccatcactc ggataattgg aacacttttg atggaaaaag acactgagtg gctggcttct
      361 ccacgaaaat atttagaatt taattcgaat aacatttagc accatgaacc tgtgcgagtc
      421 tcttctttct gaggagagac atcaagagtc gctgcgctac tgctcctgac ctctctcctc
      481 agaaagaaag gcatggcagt taggttcaca acgcaaaaca ttgtgctttt gatgaggttg
      541 aactttta
//
LOCUS       DAXLPI010000316          547 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.329, whole genome shotgun sequence.
ACCESSION   DAXLPI010000316 DAXLPI010000000
VERSION     DAXLPI010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.329"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(206..397)
                     /locus_tag="ACF3T5_002898"
     CDS             complement(206..397)
                     /locus_tag="ACF3T5_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633384.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT          184 a          102 c           69 g          192 t
ORIGIN      
        1 ttcgcttgtg gcgaaacccg acctggagtt tgtctctgta ttttgaaccc taaaaattaa
       61 tcaaacagac tgatattctc ataatcagcg cgaaataagc cattaagtac agttttccta
      121 cctatttttg ataacccgct ggttagttaa tattcctttt ccgagagaga taaatgattg
      181 gttaaatcta tatacatctc aacctttaaa aatgttttcg aaatacttca tcagtacatg
      241 tcatacaaat taccagtgca ttctcaaatt ctagttcttt aattgatttc agttgaggag
      301 cagttacttt tggcggaaat ttccattttt gtccaaacct cttcccccga tgaaatttct
      361 ttgttgcaat tattgcaatg aaaaattttt gtttcaaaaa tatacataat tacttcctcc
      421 aataaaaact atctacctct caaattattt ttctctaata agaaaaacag aaaaggtaat
      481 aagatacaac agtgatgatt cctaataaag aaaagactac aataatattt ttctaactta
      541 tcgcctt
//
LOCUS       DAXLPI010000317          543 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.330, whole genome shotgun sequence.
ACCESSION   DAXLPI010000317 DAXLPI010000000
VERSION     DAXLPI010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.330"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            22..492
                     /locus_tag="ACF3T5_002899"
     CDS             22..492
                     /locus_tag="ACF3T5_002899"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFD3633385.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKITAAIESVL"
BASE COUNT          199 a           99 c           80 g          165 t
ORIGIN      
        1 aaatagaaag gagaaaactt tatgtctatt tataattttg aaactacttt agaagacggc
       61 actgtctatt cattgaaaaa atatgcaggc aaaccgataa tcattgttaa cactgcaaca
      121 aaatgcggtc tagcacctca atttaaacaa ttagaagaac tttaccaagc ttatcagaca
      181 caaggtctgg ttatcttagg ctttccttcc aatcaattta aacaagagct agattcagcg
      241 actgaagcag cagaagcttg taaaaccact tatggcgtta ccttccctat gcatcaattg
      301 attgacgtaa atggagaaaa tacagatcct ctttttactt atcttaaaaa tgaagctcct
      361 ggtactgtca ctaaagcaat aaaatggaat tttactaaat ttttgattga tagaaatggc
      421 aaagtaatcg aacgatttgc accacaaact tctccattga aaataaccgc tgcaattgaa
      481 tccgttcttt aacttaataa aaataaaaaa agatgaacta tttctagttc atctttttgc
      541 ctt
//
LOCUS       DAXLPI010000318          533 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.331, whole genome shotgun sequence.
ACCESSION   DAXLPI010000318 DAXLPI010000000
VERSION     DAXLPI010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.331"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(170..>533)
                     /locus_tag="ACF3T5_002900"
     CDS             complement(170..>533)
                     /locus_tag="ACF3T5_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HFD3633386.1"
                     /translation="VEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVN
                     SVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKYAKVNDLSTVT
                     YEQVLSIFNSYLLFNGRK"
BASE COUNT          155 a           99 c          101 g          178 t
ORIGIN      
        1 gggtcccgag cgcttagtgg gaatttgtac cccttatcga tacaaattcc tcgtaggcgc
       61 tagggacctc tttagcttct tggaagctgt cagtagtata tctaataatt tatctccatt
      121 ccctttagta acgtgtaact ttccaaattt aaaaaagcga ctcatagaat tatttcctcc
      181 cgttaaataa tagataacta ttaaaaatag acaatacttg ctcataagta acggtactta
      241 aatcgtttac tttggcgtat ttcattgctt gatgaaactg atttttagta aacagttgac
      301 gatattctcg attgacccat tttgaaacaa agtacgtata tagcttccaa tatttatctg
      361 gaacatctgt ggtatggcgg gtaagtttta ttaagacact gtttactttt ggtttaggat
      421 gaaagcattc cgctggcagc ttaagcaatt gctgaatcga gacttgagtg tgcaagagca
      481 accctagtgt tcggtgaata tccaaggtac gcttgtagaa tccttcttca aca
//
LOCUS       DAXLPI010000319          521 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.332, whole genome shotgun sequence.
ACCESSION   DAXLPI010000319 DAXLPI010000000
VERSION     DAXLPI010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.332"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            94..>521
                     /locus_tag="ACF3T5_002901"
     CDS             94..>521
                     /locus_tag="ACF3T5_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFD3633387.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDR"
BASE COUNT          197 a           61 c          109 g          154 t
ORIGIN      
        1 tttttatgtt tattaacttc ggaagactaa aattgttact ggtaatgata catttgtaaa
       61 aataaaaaag atgattttta ggaagtgaag agagtgcata aaattagtgt tatagtaccc
      121 gcatataacg tagaagacta tttagaaaac tgcttagaat ctatttccaa ccagacacat
      181 tcaaatattg aaataatagt agtcaatgat ggttccccgg atcgaagtgg ggaaattgca
      241 gaagaatata aaaagaaaga tatgcgatta aaagtgatac atcaagtgaa tggtggttta
      301 agtgcggcta ggaattctgg tcttgattct gcaacaggag attttgtgtg ctttattgac
      361 agcgatgatt ggatagaacc agactatctt gagttactat attttggcac gcaaacttat
      421 ggagcagata tctcggtgct aaaaataaaa aagatttcaa gtttagaaca actaaaagaa
      481 catacaattg ctgagagaaa atgggaaata tttgatagag a
//
LOCUS       DAXLPI010000320          494 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.333, whole genome shotgun sequence.
ACCESSION   DAXLPI010000320 DAXLPI010000000
VERSION     DAXLPI010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.333"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>494)
                     /locus_tag="ACF3T5_002902"
     CDS             complement(<1..>494)
                     /locus_tag="ACF3T5_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFD3633388.1"
                     /translation="IDICENKILTSAFFEQNGFKTPEILNSSEITKFPVFIKPLNGSS
                     SVNTFIVNNEKELLFFSEYIDNPLIQEYILGEEYTIDVCLNFDSTPVSIVPRKRIATR
                     GGEILKGQIIKDRELIETTKKMLEKLNAIGHITIQCIKNEKGIFYLEINARYGGGAPI
                     SIKSG"
BASE COUNT          161 a           84 c           58 g          191 t
ORIGIN      
        1 cctgatttaa tgctaatggg ggctcctcca ccgtatcttg catttatttc taaataaaat
       61 attccctttt cgttttttat acattgaata gtaatatgtc cgattgcatt taatttttcc
      121 aacatctttt tcgtagtctc aattagttct ctatctttaa taatctgacc cttcagtatt
      181 tccccacctc tagttgctat tctttttcta ggaacaatac ttactggtgt cgaatcaaag
      241 tttaagcaaa catctatagt atattcttca cctaaaatat actcttgaat aagaggatta
      301 tctatatatt cactaaaaaa caacaattct ttttcattat ttacaataaa tgtgttaaca
      361 gatgaactac cattcagagg ttttataaat acaggaaatt ttgtaatttc agaagagttt
      421 aaaatttcag gagttttaaa accattttgt tcaaaaaaag cagaagttaa aattttgttc
      481 tcacaaatgt cgat
//
LOCUS       DAXLPI010000321          469 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.334, whole genome shotgun sequence.
ACCESSION   DAXLPI010000321 DAXLPI010000000
VERSION     DAXLPI010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.334"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          162 a           66 c           79 g          162 t
ORIGIN      
        1 gtcttcctgt gtatgtaggg ttattcacgg cgattgcaag tattataggg ctgaaaaaaa
       61 ataaatttta atgtgggaat tattattatt tctctaattc tatttgagca atttaaaata
      121 tgaaaatcaa taaagagaaa gttttaagtt gttaagtaaa ttaaatttcg attggaagca
      181 tagttgaata agcctaacat gttacgaaag ataaataacc tacttttcat ttctagaaga
      241 gaatgtcttg atgcttataa acagatttag ctccctatcc acttcaagtc taattctttt
      301 gtatgcaaat aaagtatttt taggagagtg ttctcattag agaacactct ttttttgttt
      361 tgaaaactac ttactatacg ggtagtttca aagtaagcgc cccatagaac agtaccttac
      421 taacaaatgc acctcaagat atacttgagc tatcaagaag tctggaggt
//
LOCUS       DAXLPI010000322          465 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.335, whole genome shotgun sequence.
ACCESSION   DAXLPI010000322 DAXLPI010000000
VERSION     DAXLPI010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.335"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            49..324
                     /locus_tag="ACF3T5_002903"
     CDS             49..324
                     /locus_tag="ACF3T5_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633389.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            complement(353..>465)
                     /locus_tag="ACF3T5_002904"
     CDS             complement(353..>465)
                     /locus_tag="ACF3T5_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3633390.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT          190 a           65 c           73 g          137 t
ORIGIN      
        1 aaaggcgcta tgatttccat atgaaaaaaa taaagtcagg tgaaaaaaat gaaacttgag
       61 gataaaaaag tcattttgaa gaaaatcaaa cgactaccag accaaataga tggctatacg
      121 atctgttctt tcaaaaaaga caggagtata aaagcttata cgcaaaacac agaaaaagga
      181 ataatctatt tcctacatga agaaggttac gatacccaaa cctttatgtt tactgaaaaa
      241 aaagaatttc ataaacagat gaaaaaactg attgaaaaag aatttccaag aagtcataaa
      301 ctctatgtga atcagcaatt ttaacttcta actgttcttt atttatgatt tgtcaaatta
      361 agctagaata aaatgcttct tgtacatagc tcaaaaatat ttcgattggt gttctgtaat
      421 tcaatgattt tcttggaata tgattacgtt gattgctgac actgg
//
LOCUS       DAXLPI010000323          435 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.336, whole genome shotgun sequence.
ACCESSION   DAXLPI010000323 DAXLPI010000000
VERSION     DAXLPI010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.336"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>435)
                     /locus_tag="ACF3T5_002905"
     CDS             complement(<1..>435)
                     /locus_tag="ACF3T5_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3633391.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLS
                     YNEELRTAYHFCQLLRYYFVKRGTEGFQETLKTISSTLPQSFK"
BASE COUNT          122 a           77 c           97 g          139 t
ORIGIN      
        1 tttaaacgac tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa agccttccgt
       61 acctcgttta acgaaataat agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc
      121 ttcgttgtag cttaacagct catcaacgat ttctgtttga ctaattggtc gtttaaacag
      181 tcgatgataa tgccgtttga gtggctccca tgtcgtggaa tctttgagta agagtttcca
      241 atatcgcttg agccgacggt acttcttctg attttcagag tgtgaggttc gaaaacgatt
      301 catgatctgg acacgtaatt gattgaagct gcgattgatg tgttggacaa tatgaaagcg
      361 gtcagttacg atctccgcat ttggaaagac tgttttaagc aactgacaat agctggcatt
      421 catgtccatc actag
//
LOCUS       DAXLPI010000324          416 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.337, whole genome shotgun sequence.
ACCESSION   DAXLPI010000324 DAXLPI010000000
VERSION     DAXLPI010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.337"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            131..355
                     /locus_tag="ACF3T5_002906"
                     /pseudo
     CDS             131..355
                     /locus_tag="ACF3T5_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          141 a           59 c           72 g          144 t
ORIGIN      
        1 tttttagata caatatcgat cagttttgag gaaatgaaat tctagatatt ttttgctaca
       61 tagagttact ttcaatattt tttaacgtaa ttaggcagaa gtcactcatc ctctataggt
      121 ttgtgatgaa attgccattg atccatttaa gtaagagcaa aaaactggag gtttatcagg
      181 taattggtct aatggattta agtatgcggt caccgtgtat agagttgctt atgagattca
      241 agaaaataaa gttattccag tattgctata tggaacacat aacaattttt atgaattagc
      301 cgagaacact cgcaacttta ttcgtgtaga tgaatcggct tcggttgagt tataacttta
      361 gctatatcaa tcaaccgacc gacatatgtt aaatctatat ataataaaaa taccta
//
LOCUS       DAXLPI010000325          415 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.338, whole genome shotgun sequence.
ACCESSION   DAXLPI010000325 DAXLPI010000000
VERSION     DAXLPI010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.338"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..339)
                     /locus_tag="ACF3T5_002907"
     CDS             complement(<1..339)
                     /locus_tag="ACF3T5_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633392.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DAXLPI010000326          403 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.339, whole genome shotgun sequence.
ACCESSION   DAXLPI010000326 DAXLPI010000000
VERSION     DAXLPI010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.339"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..382
                     /locus_tag="ACF3T5_002908"
     CDS             <1..382
                     /locus_tag="ACF3T5_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3633393.1"
                     /translation="KRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWL
                     KTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNR
                     NRIHSSIHYLTPQEFEELAKEKMA"
BASE COUNT          159 a           76 c           70 g           98 t
ORIGIN      
        1 aaaacgaatg actgtggatt gtgtcattca aacattgaat aaagcaaaaa tacattatca
       61 cattccagaa ggaatgattc tacacactga cttgggcagc caatacacag caagagaagt
      121 ggaacaatgg cttaaaacca acaaaataag gcattcttat agtagaaaag gaacacctta
      181 tgataatgct ggaatcgaat ctttccatgc ctcattgaaa aaagaagaag tctacacgac
      241 tagctactca gattttgaag aagcaaatcg agcgctattt agctacattg aaggatttta
      301 taaccgaaat cgaatccata gctcgattca ctatctaacc ccacaggaat ttgaagaatt
      361 agcaaaagaa aaaatggctt aaccgtctct actaaaatgt gtc
//
LOCUS       DAXLPI010000327          396 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.340, whole genome shotgun sequence.
ACCESSION   DAXLPI010000327 DAXLPI010000000
VERSION     DAXLPI010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.340"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..380
                     /locus_tag="ACF3T5_002909"
     CDS             <1..380
                     /locus_tag="ACF3T5_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFD3633394.1"
                     /translation="EYKDYYTTGYKTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKI
                     LNYEKGNRGVRFNFETDDAGAGRFKYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEL
                     HNWPTFYPASLSKHEIAQEMMAH"
BASE COUNT          140 a           59 c           69 g          128 t
ORIGIN      
        1 ccgagtacaa agattattat actactggct ataagacaga tattgatact atcaacatta
       61 aagacaacac cattgatttt gtagtcaacg gtgaacatca tcaatacaca tacaaatatg
      121 ttggatacaa aattttgaat tatgaaaaag gaaaccgtgg tgttcgtttt aattttgaaa
      181 ctgatgatgc aggtgctggt cgttttaaat atattcaatt tagtgatcat ggaattgcac
      241 caagtaaggc agagcatttc catattttct ttggtggaga aagtcaagaa aaactctata
      301 atgaattgca taattggcct actttctatc cagcttcatt gtctaaacat gaaattgctc
      361 aagaaatgat ggctcattaa aattcaattt ttggag
//
LOCUS       DAXLPI010000328          392 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.341, whole genome shotgun sequence.
ACCESSION   DAXLPI010000328 DAXLPI010000000
VERSION     DAXLPI010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.341"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            143..>392
                     /locus_tag="ACF3T5_002910"
     CDS             143..>392
                     /locus_tag="ACF3T5_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633395.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDL"
BASE COUNT          176 a           48 c           55 g          113 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt at
//
LOCUS       DAXLPI010000329          388 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.342, whole genome shotgun sequence.
ACCESSION   DAXLPI010000329 DAXLPI010000000
VERSION     DAXLPI010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.342"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          134 a           56 c           57 g          141 t
ORIGIN      
        1 ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg tacattatgc
       61 tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac ttaatccata
      121 gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca ccccaaacca
      181 cttccccata agtgtattac gctttaaact cttttatatt tgaattcact aagaagcata
      241 ccatatattt gaatttacta agaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttataatta ggatttagag taattaatta gatgtttatt ttataggact gatactatag
      361 attacaaata gtatcgtatt tcgcaatc
//
LOCUS       DAXLPI010000330          385 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.343, whole genome shotgun sequence.
ACCESSION   DAXLPI010000330 DAXLPI010000000
VERSION     DAXLPI010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.343"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          164 a           50 c           38 g          133 t
ORIGIN      
        1 aaacaaaaat aaataaagtc ggatttttct gataattcaa cgcaaaaaaa cgaacaattt
       61 ttcaataaat cagtcttcta aaatagataa cctaaaaaaa acgaacaaac aaataaaatg
      121 actgtccttc attcactaaa catattttta ttttttatat tgttaaaaat tcacaattat
      181 tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt tttaacggca
      241 tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt cataatattt
      301 cccatattgt gcaaaaaaat acaaggtgga ggctctacta tgattgaaga atatattgag
      361 aaaaatattt taagacagct gtttt
//
LOCUS       DAXLPI010000331          381 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.344, whole genome shotgun sequence.
ACCESSION   DAXLPI010000331 DAXLPI010000000
VERSION     DAXLPI010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.344"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>381)
                     /locus_tag="ACF3T5_002911"
     rRNA            complement(<1..>381)
                     /locus_tag="ACF3T5_002911"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           85 a          110 c           94 g           92 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc a
//
LOCUS       DAXLPI010000332          378 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.345, whole genome shotgun sequence.
ACCESSION   DAXLPI010000332 DAXLPI010000000
VERSION     DAXLPI010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.345"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            218..>378
                     /locus_tag="ACF3T5_002912"
     CDS             218..>378
                     /locus_tag="ACF3T5_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633396.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLF"
BASE COUNT          151 a           39 c           60 g          128 t
ORIGIN      
        1 aataaaaaca attacagcta tgatgtttaa gataaaacaa ttatgaaatc gatatccgtt
       61 tatatactta tcataagcta gaagcaaata actagcctat gtatgtaagt gagattatat
      121 ttattaaaag gcttcttttg ttcgatcagt tatagtcgaa cgagagaagt ttattctttg
      181 attaaaataa acaaaagtaa cttattgagg tgaaatgatg caaacttttt taactaaaac
      241 atctttcaaa aaagtagttt tattagatta tttgttagaa aaagacaatt ggtgtgcaat
      301 ggaagaactt agaaatttac ttaatgttac ggagaagtca gtcttacact atatagaaga
      361 actagaggat ttatttaa
//
LOCUS       DAXLPI010000333          378 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.346, whole genome shotgun sequence.
ACCESSION   DAXLPI010000333 DAXLPI010000000
VERSION     DAXLPI010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.346"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          142 a           45 c           80 g          111 t
ORIGIN      
        1 cattggtcgt tgaatgggct gaatagtagt gtataaaaca attttgtgga gcgtgaagct
       61 tttatggtag aggaaaatac gaataaagtg atgttgataa aaagattcct atcttgaata
      121 tgaatcgaga tgtcttgaaa atgaatgagt ttgaccaatc attatcctat cgaaatttac
      181 agtttcgttc gattggtctg tcaattaatg aaaaagtaaa aaagaatatg ttctaggaag
      241 agggcggcac atctaaattt ttaagtaacg gacagttcaa atatcaagca ttgaaagagg
      301 ctatcctact gtttgaagga atacgaatat ataaaaacga caaagaggaa aaagtagttc
      361 attaaatccc ttcaagtg
//
LOCUS       DAXLPI010000334          376 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.347, whole genome shotgun sequence.
ACCESSION   DAXLPI010000334 DAXLPI010000000
VERSION     DAXLPI010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.347"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(139..>376)
                     /locus_tag="ACF3T5_002913"
     CDS             complement(139..>376)
                     /locus_tag="ACF3T5_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HFD3633397.1"
                     /translation="LVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVSTWSKNG
                     ITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT           94 a           72 c           46 g          164 t
ORIGIN      
        1 gtatcatttc gttttgcttt ttgtgcgcct aaatttccca caatcactca cttctttcta
       61 tttcttctta ctcttatttt atcatcaaca atcacaaatc acaagtgatt tgtgattaat
      121 cacttgtgat ttgtgattct attctttttc gttttctaat tgaataattc gctcaagcat
      181 ttcaaaaaat acgttcttat acatggataa aacttttttg tcatagcctt tatgctctgt
      241 aatgccgttt ttagaccagg tacttacttt attacttcgc ttgataatat tttggaaaac
      301 caagttatct tctttatgtt gcttatacag ttcttccagg tttgatttta tcgttgcgct
      361 gtccgtatca actaaa
//
LOCUS       DAXLPI010000335          362 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.349, whole genome shotgun sequence.
ACCESSION   DAXLPI010000335 DAXLPI010000000
VERSION     DAXLPI010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.349"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>362)
                     /locus_tag="ACF3T5_002914"
     rRNA            complement(<1..>362)
                     /locus_tag="ACF3T5_002914"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           81 a          114 c           68 g           99 t
ORIGIN      
        1 tctcactaga agcttttctc ggcagtgtga catcaggaac ttcggtacta ttatttccct
       61 ccccatcaca gcttgtcctt aagaaaagaa gcatttgact cctcttcaga cttactgctt
      121 ggacagacat ttccgatcgt ctgcattcct tagcctcctg cgtccctcca ttgctcaaac
      181 aaaaacaacg ggtacaggaa tatcaacctg ttatccatcg cctacgcctg tcggcctcgg
      241 cttaggtccc gactaaccct gggcggacga gccttcccca ggaaacctta gtcattcggt
      301 ggacaggatt ctcacctgtc tttcgctact cataccggca ttctcacttc taagcgctcc
      361 ag
//
LOCUS       DAXLPI010000336          356 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.350, whole genome shotgun sequence.
ACCESSION   DAXLPI010000336 DAXLPI010000000
VERSION     DAXLPI010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.350"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..195
                     /locus_tag="ACF3T5_002915"
     CDS             <1..195
                     /locus_tag="ACF3T5_002915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFD3633398.1"
                     /translation="PVTPETSNYKTLQIQKSKVNYKSETNKTLPKTGEAKSSGVFVGI
                     IVLLTSIFAGFKLNLKRKKG"
BASE COUNT          148 a           36 c           61 g          111 t
ORIGIN      
        1 ccggtaacgc cagagactag taattataaa actctacaaa tacaaaagag taaggtgaat
       61 tataaatctg aaacaaataa aactcttcca aaaactggag aggcaaaatc atctggagta
      121 tttgtaggta taatagtttt attgacttct atttttgctg gatttaaatt aaatttgaaa
      181 agaaaaaaag gatagaaaat agatctgaga tagaatactt agtgttctat ctcaggttgt
      241 aattatgttt tatatcactc aagtattaag gagggattat gataacttta gaagagaaat
      301 atatataatc tttaggcaag aaatgtataa gaaaagagtc tatttaaatc ataagt
//
LOCUS       DAXLPI010000337          355 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.351, whole genome shotgun sequence.
ACCESSION   DAXLPI010000337 DAXLPI010000000
VERSION     DAXLPI010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.351"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            2..>355
                     /locus_tag="ACF3T5_002916"
     CDS             2..>355
                     /locus_tag="ACF3T5_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HFD3633399.1"
                     /translation="MESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPILIDQAS
                     EHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITKTSPYNK
                     TDEENFHLIVTTLQTE"
BASE COUNT          144 a           50 c           47 g          114 t
ORIGIN      
        1 tatcgaatct attgattcta caatcgatag attaaaaaaa gaaattcgat tttataatag
       61 agtaattggt atcggaaata ttataaaaat agtcttatca gctagtatcc caatattaat
      121 tgatcaggct tccgaacaca ggtctttact attgattgtt tcaattgctt cagcaataat
      181 tacaattata caaagtggta tgtctgcatt taattatcaa gataaagtac agacttcgac
      241 agagttatta atgaaaattg aaaaagaaaa gttgttatat ataacgaaaa catccccata
      301 taataaaaca gatgaagaaa atttccattt aatcgttaca acccttcaaa ctgaa
//
LOCUS       DAXLPI010000338          349 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.352, whole genome shotgun sequence.
ACCESSION   DAXLPI010000338 DAXLPI010000000
VERSION     DAXLPI010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.352"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(44..>349)
                     /locus_tag="ACF3T5_002917"
     CDS             complement(44..>349)
                     /locus_tag="ACF3T5_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFD3633400.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           98 a           86 c           64 g          101 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggt
//
LOCUS       DAXLPI010000339          340 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.354, whole genome shotgun sequence.
ACCESSION   DAXLPI010000339 DAXLPI010000000
VERSION     DAXLPI010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.354"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            43..165
                     /locus_tag="ACF3T5_002918"
     CDS             43..165
                     /locus_tag="ACF3T5_002918"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633401.1"
                     /translation="MHKPFPDPIQKAIDELGIKNKKRFILVIHYMTCKVRKLVG"
     gene            138..269
                     /locus_tag="ACF3T5_002919"
     CDS             138..269
                     /locus_tag="ACF3T5_002919"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633402.1"
                     /translation="MQSAKACGIAFGLAKWGAKNIENFQSVDIIIDSPQEILDLLEK"
BASE COUNT          123 a           45 c           64 g          108 t
ORIGIN      
        1 aatctatatt ttgatataca aattacagct tcagatacta agttgcataa accttttccg
       61 gaccccatac aaaaggcaat tgatgaattg ggcataaaaa ataagaaacg atttatattg
      121 gtgattcatt atatgacatg caaagtgcga aagcttgtgg gatagcattt ggtttagcta
      181 aatggggagc caaaaatata gaaaattttc aaagtgtaga tataattatt gattctcctc
      241 aagaaatact tgatttgtta gaaaaataga caaagagcgt gagataagga tagggtctca
      301 cgctctttgt ttttcgccat ttaaagattg aaatttttgc
//
LOCUS       DAXLPI010000340          335 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.355, whole genome shotgun sequence.
ACCESSION   DAXLPI010000340 DAXLPI010000000
VERSION     DAXLPI010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.355"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            4..>335
                     /locus_tag="ACF3T5_002920"
     CDS             4..>335
                     /locus_tag="ACF3T5_002920"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFD3633403.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGG"
BASE COUNT          122 a           30 c           57 g          126 t
ORIGIN      
        1 tttatggaag aagttttaat tgatgataat atggtttttg atattgataa tttaaaagga
       61 tttcttaatg ataccagttc atttgggttt atagctaaag aaaataataa aattatagga
      121 tttgcatatt gctatacact tttaagacct gatggaaaaa caatgtttta tttacactca
      181 ataggaatgt tacctaacta tcaagacaaa ggttatggtt caaaattatt atcttttatt
      241 aaggaatatt ctaaagagat tggttgttct gaaatgtttt taataactga taaaggtaat
      301 cctagagctt gccatgtata tgaaaaatta ggtgg
//
LOCUS       DAXLPI010000341          323 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.357, whole genome shotgun sequence.
ACCESSION   DAXLPI010000341 DAXLPI010000000
VERSION     DAXLPI010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.357"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>323)
                     /locus_tag="ACF3T5_002921"
     rRNA            complement(<1..>323)
                     /locus_tag="ACF3T5_002921"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           64 a           96 c           74 g           89 t
ORIGIN      
        1 ataaatccgg acaacgcttg ccacctacgt attaccgcgg ctgctggcac gtagttagcc
       61 gtggctttct ggttagatac cgtcaaggga tgaacagtta ctctcatcct tgttcttctc
      121 taacaacaga gttttacgat ccgaaaacct tcttcactca cgcggcgttg ctcggtcaga
      181 ctttcgtcca ttgccgaaga ttccctactg ctgcctcccg taggagtttg ggccgtgtct
      241 cagtcccaat gtggccgatc accctctcag gtcggctatg catcgtggcc ttggtgagcc
      301 gttacctcac caactagcta atg
//
LOCUS       DAXLPI010000342          317 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.358, whole genome shotgun sequence.
ACCESSION   DAXLPI010000342 DAXLPI010000000
VERSION     DAXLPI010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.358"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>317
                     /locus_tag="ACF3T5_002922"
     CDS             <1..>317
                     /locus_tag="ACF3T5_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFD3633404.1"
                     /translation="NTLLRTELTAFLDYEKYDRTGFNSGNSRNGSYFRSIKTEYGELT
                     LEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEKGVTMSEIADLIEKMYGHHYTPQ
                     TMS"
BASE COUNT          116 a           60 c           51 g           90 t
ORIGIN      
        1 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
       61 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      121 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      181 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      241 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      301 ctccacaaac catgtcc
//
LOCUS       DAXLPI010000343          316 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.359, whole genome shotgun sequence.
ACCESSION   DAXLPI010000343 DAXLPI010000000
VERSION     DAXLPI010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.359"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..192)
                     /locus_tag="ACF3T5_002923"
     CDS             complement(<1..192)
                     /locus_tag="ACF3T5_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFD3633405.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGK"
BASE COUNT          107 a           67 c           32 g          110 t
ORIGIN      
        1 cttccctgga attgctccac ctaaaaaacc acctatagac attcctgcaa tacaagtagt
       61 tgccttggcc caatcgaccg tacatttatt tttagtgcaa tacactccat ttccataata
      121 ttttccacta tgagtggtac ccccatagat aagttgtgtc tctttaatag acaaaatttt
      181 taaatgtttc ataatctaat cacccctaaa ttaatgtttt tagactatta ttctaacatt
      241 cattaaattt caaaaaacaa ttccttcttg aaacgtagca aaataattcc tgaataagaa
      301 tttaatcact tttaag
//
LOCUS       DAXLPI010000344          305 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.360, whole genome shotgun sequence.
ACCESSION   DAXLPI010000344 DAXLPI010000000
VERSION     DAXLPI010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.360"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACF3T5_002924"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="ACF3T5_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFD3633406.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DAXLPI010000345          280 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.361, whole genome shotgun sequence.
ACCESSION   DAXLPI010000345 DAXLPI010000000
VERSION     DAXLPI010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.361"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          100 a           24 c           45 g          111 t
ORIGIN      
        1 ttgatacagt tcgtagagtg ttaaaacaag accgggattg ttgttttatc aaggtcaata
       61 ctggaagaag tagacgttta gcaaaacgat ataatagttt gattaattag ttgtaacagt
      121 agagcagatt aattttcttt tattaggatt tgatctgctc tttattatgt ataggaatta
      181 acatattcga aaaaatatta caaaattgta atgctattat ttataattaa ttattttaca
      241 atgctaaaat tgatgataat attttattgt atctgttaat
//
LOCUS       DAXLPI010000346          278 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.362, whole genome shotgun sequence.
ACCESSION   DAXLPI010000346 DAXLPI010000000
VERSION     DAXLPI010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.362"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            67..>278
                     /locus_tag="ACF3T5_002925"
     CDS             67..>278
                     /locus_tag="ACF3T5_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010709180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633407.1"
                     /translation="MFYQRSEKKFKYAIKKKKGGGGAASFIIGAMFLGIGLISCPTVS
                     FASETTEINQSTEIQSTLNNSILKES"
BASE COUNT           99 a           32 c           52 g           95 t
ORIGIN      
        1 cttataaaat gaacgttcgt ttttaggatt tctttttgat aactttttat gttaggagga
       61 taaagtatgt tttatcaaag aagcgagaag aagtttaagt acgcaattaa aaagaaaaaa
      121 ggaggaggtg gagcggcttc tttcattatc ggtgcaatgt ttttaggaat aggattaatt
      181 tcttgtccta cagtttcttt tgctagtgaa acaacagaga taaatcaatc aactgaaatc
      241 caatctactt tgaataattc tattctaaaa gaaagtaa
//
LOCUS       DAXLPI010000347          273 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.363, whole genome shotgun sequence.
ACCESSION   DAXLPI010000347 DAXLPI010000000
VERSION     DAXLPI010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.363"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            30..>273
                     /locus_tag="ACF3T5_002926"
     CDS             30..>273
                     /locus_tag="ACF3T5_002926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFD3633408.1"
                     /translation="MPDESKRKDWIKDERLWNDWKGIAKALEKNDFVLSTRNYSLTKK
                     DEAELYEMFKEYKDFLGINQLKGKRYENIVQKIGGMK"
BASE COUNT          130 a           19 c           54 g           70 t
ORIGIN      
        1 agaataaata taatatggat atccaagata tgccagatga atcaaaaaga aaagattgga
       61 taaaagatga aagactttgg aatgactgga aagggatagc taaagcttta gaaaaaaatg
      121 actttgtttt atctactaga aattatagtt taacgaaaaa agatgaagct gaactatatg
      181 agatgtttaa agaatataaa gactttttgg gaataaatca attaaaagga aagaggtacg
      241 aaaatattgt acaaaaaata ggaggaatga aat
//
LOCUS       DAXLPI010000348          265 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.364, whole genome shotgun sequence.
ACCESSION   DAXLPI010000348 DAXLPI010000000
VERSION     DAXLPI010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.364"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>265
                     /locus_tag="ACF3T5_002927"
     CDS             <1..>265
                     /locus_tag="ACF3T5_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633409.1"
                     /translation="KLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILM
                     ELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL"
BASE COUNT           83 a           56 c           58 g           68 t
ORIGIN      
        1 aaagttaact attttagaat taaaacgtac ccgttttctc tgccatgatt gtggaaggac
       61 cttttctgcg aaaacggact tagttgatga acatcatcaa ctaacaaagg aactgaagca
      121 ggcgattctc atggaactgt acgaaaatca atcacgtaaa ctaatcgcta agaagtactt
      181 cgtctcggat gggacggtga cacggatctt gcgtgaagca acgaagcatt accagccacg
      241 tatgaacttc cttccgacgg tgttg
//
LOCUS       DAXLPI010000349          259 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.365, whole genome shotgun sequence.
ACCESSION   DAXLPI010000349 DAXLPI010000000
VERSION     DAXLPI010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.365"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          104 a           42 c           27 g           86 t
ORIGIN      
        1 ctctactaca ccatatctat tgacaatcaa ccaaataaaa tacagtattt cacgatatag
       61 tgtgataaat aacaaaatag aagcaataaa gcttcttgtg ttaaaacaat tataaaagaa
      121 ctaaataatt agcccttcta tctataaaaa agtcaatagc gtcactagga tttcctaata
      181 acgctattga ttttcttttc ctctgaaatt aaatcagaaa gatttttcaa taatgataac
      241 tgttttgatt aacagcttt
//
LOCUS       DAXLPI010000350          255 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.366, whole genome shotgun sequence.
ACCESSION   DAXLPI010000350 DAXLPI010000000
VERSION     DAXLPI010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.366"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>255)
                     /locus_tag="ACF3T5_002928"
     CDS             complement(<1..>255)
                     /locus_tag="ACF3T5_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFD3633410.1"
                     /translation="AEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVAELL
                     KKANTIIVATDSDREGENIAWSIIHKANAFSKDKTFKRLW"
BASE COUNT           67 a           51 c           38 g           99 t
ORIGIN      
        1 atccatagtc ttttaaatgt tttatctttt gaaaaggcat ttgctttatg gataatcgac
       61 caggcgatat tttcaccttc tctgtcgcta tctgttgcaa caataattgt atttgccttt
      121 ttgagaagtt ctgcaacaat tttaaactgc tttcccttat cttttgcaac ttcaaaatcg
      181 tatcgatcag gaaaaatcgg caaagattca agtttccaat tttgccactt ttcgtcataa
      241 tgacctggtt ctgct
//
LOCUS       DAXLPI010000351          252 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.367, whole genome shotgun sequence.
ACCESSION   DAXLPI010000351 DAXLPI010000000
VERSION     DAXLPI010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.367"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT           87 a           22 c           51 g           92 t
ORIGIN      
        1 tttaaacggt agttacttag ttcttctttg atttgcgtga aatagagggg atttattttt
       61 ctggttacgt gattaaaaaa tccaaaaaat gagcatagga acggttatga ttttagggtg
      121 tgttttaatg agatattgtg atttcattaa caagtgtatg ataatcggtt cttttgcaaa
      181 gttttccaaa aaatctattt tagtgtaaaa ttgagaaaaa aacagagagg acagagtaat
      241 gaatcatttt aa
//
LOCUS       DAXLPI010000352          250 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.368, whole genome shotgun sequence.
ACCESSION   DAXLPI010000352 DAXLPI010000000
VERSION     DAXLPI010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.368"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            18..>250
                     /locus_tag="ACF3T5_002929"
     CDS             18..>250
                     /locus_tag="ACF3T5_002929"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633411.1"
                     /translation="MVGKNNKKLLELKSNPIIKKWRISRLKTGVASIVIFSGVLLSST
                     IFIPLHTVSAEETVTTTYYDQSKNVSDQLTNIN"
BASE COUNT          103 a           29 c           38 g           80 t
ORIGIN      
        1 gtagaaagga taattttatg gttggaaaaa acaataaaaa gttattagaa ttaaaaagca
       61 atccaattat caaaaagtgg agaattagtc gactgaaaac aggagttgca tcaattgtca
      121 tatttagcgg ggtattatta agttctacta tttttatacc tttacatact gtttcagcag
      181 aagaaacagt aactactact tattatgacc aatctaaaaa tgttagtgat caattaacaa
      241 atattaatat
//
LOCUS       DAXLPI010000353          244 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.369, whole genome shotgun sequence.
ACCESSION   DAXLPI010000353 DAXLPI010000000
VERSION     DAXLPI010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.369"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT          100 a           26 c           48 g           70 t
ORIGIN      
        1 aaatagggtt gacgcacttt tgatgtcatg ttataataac gaagtcgttt ggatgaggta
       61 gctaataaag cttgatttta tcgaaaaaaa cttcgaaaaa gttgttgaca aataacaata
      121 aagatgatat gataatcaag tcgctgctaa tgagtgacaa agacgtttag aaaaaataaa
      181 caaagaagtt gttgacattc tcttctgaat ctgataagat taaagagttg ttgaaacaaa
      241 aaga
//
LOCUS       DAXLPI010000354          241 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.370, whole genome shotgun sequence.
ACCESSION   DAXLPI010000354 DAXLPI010000000
VERSION     DAXLPI010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.370"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            55..>241
                     /locus_tag="ACF3T5_002930"
     CDS             55..>241
                     /locus_tag="ACF3T5_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFD3633412.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQ"
BASE COUNT           96 a           23 c           44 g           78 t
ORIGIN      
        1 agtgttagtt aaagatgaat atacttttta tagaaaggaa aagaatgtaa ttaagtggaa
       61 aaaatagtaa ttattggttc aaaccattca gggctagccg cagctcgatt tctaaactta
      121 agcgattcaa attatgaagt tattctaatc gataaaaaat cagaaattag ttacttggaa
      181 tgtgggacat cgttatttat aaaaggagaa gtaagtaatt tagaagatct tttttatcaa
      241 a
//
LOCUS       DAXLPI010000355          241 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.371, whole genome shotgun sequence.
ACCESSION   DAXLPI010000355 DAXLPI010000000
VERSION     DAXLPI010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.371"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..220)
                     /locus_tag="ACF3T5_002931"
     CDS             complement(<1..220)
                     /locus_tag="ACF3T5_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFD3633413.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT           63 a           45 c           46 g           87 t
ORIGIN      
        1 caggaaaaat gatttcggtt ctcccacaac gtcgcttatt ttctttgtat tgttcaaagt
       61 attcaagtgc tgttccacca catttgagaa agttatagac attgtaaatt gtttggcgtc
      121 cacgtttaag ctgcttcgca acgatagcaa ccggagtttc ttgatgaaaa tatgcttcta
      181 tcattacaag ctcgtttggt gtaagatggg tataagtcat ttatgttcac tctccttgta
      241 t
//
LOCUS       DAXLPI010000356          236 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.373, whole genome shotgun sequence.
ACCESSION   DAXLPI010000356 DAXLPI010000000
VERSION     DAXLPI010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.373"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..>236)
                     /locus_tag="ACF3T5_002932"
     CDS             complement(<1..>236)
                     /locus_tag="ACF3T5_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633414.1"
                     /translation="VVEKENTRKPYTEVENGVSYIVPKGKRRQATLDAMKTNYVHELE
                     RFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEP"
BASE COUNT           57 a           57 c           30 g           92 t
ORIGIN      
        1 ggctctttta ccacttttgt taaggtgtta cgcaaatcac ttttccgcgt caataaattt
       61 cgtttctcca taccaatcat tcgatccttt tcataagaaa aacgttctaa ctcatgtaca
      121 taattggtct tcattgcgtc taatgttgct tgtcttctct tacctttcgg tacgatataa
      181 ctcacaccat tttcaacctc cgtgtaaggt ttacgcgtat tttctttttc aacaac
//
LOCUS       DAXLPI010000357          233 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.374, whole genome shotgun sequence.
ACCESSION   DAXLPI010000357 DAXLPI010000000
VERSION     DAXLPI010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.374"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
BASE COUNT           79 a           35 c           45 g           74 t
ORIGIN      
        1 tgagaaaaag catggaatgc gctgcatcag gtatcgaggc ttagaaaaaa atcaggtgca
       61 tacgatgctt gtttttgccg aaatgagtct gaaaaaattg gtcacttagc agtgggagca
      121 tatgtcttca tattttttat tatacaaaaa tagacaaaag tatcagtcta cactcttagt
      181 aaagaggaaa ctgatacttt tgtctacagt atgatatttc ttacatctac ttt
//
LOCUS       DAXLPI010000358          229 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.375, whole genome shotgun sequence.
ACCESSION   DAXLPI010000358 DAXLPI010000000
VERSION     DAXLPI010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.375"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..185
                     /locus_tag="ACF3T5_002933"
     CDS             <1..185
                     /locus_tag="ACF3T5_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFD3633415.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           61 a           51 c           57 g           60 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggagg
//
LOCUS       DAXLPI010000359          227 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.376, whole genome shotgun sequence.
ACCESSION   DAXLPI010000359 DAXLPI010000000
VERSION     DAXLPI010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.376"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            1..227
                     /locus_tag="ACF3T5_002934"
                     /pseudo
     CDS             1..227
                     /locus_tag="ACF3T5_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DAXLPI010000360          225 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.denovo.377, whole genome shotgun sequence.
ACCESSION   DAXLPI010000360 DAXLPI010000000
VERSION     DAXLPI010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.denovo.377"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..175)
                     /locus_tag="ACF3T5_002935"
     rRNA            complement(<1..175)
                     /locus_tag="ACF3T5_002935"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           50 a           60 c           49 g           66 t
ORIGIN      
        1 cctgtttcca agtgttatcc ccttctgatg ggcaggttac ccacgtgtta ctcacccgtt
       61 cgccactcct ctttttccgg tggagcaagc tccggtggaa aaagaagcgt tcgacttgca
      121 tgtattaggc acgccgccag cgttcgtcct gagccaggat caaactctca taaaaagttc
      181 gaacaatctt gcgattgtta agctcaattt gtttgctagc atatt
//
LOCUS       DAXLPI010000361        10334 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.091, whole genome shotgun sequence.
ACCESSION   DAXLPI010000361 DAXLPI010000000
VERSION     DAXLPI010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.091"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(<1..153)
                     /locus_tag="ACF3T5_002936"
     CDS             complement(<1..153)
                     /locus_tag="ACF3T5_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAT RNA binding domain-containing protein"
                     /protein_id="HFD3633416.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEP"
     gene            complement(495..1367)
                     /locus_tag="ACF3T5_002937"
     CDS             complement(495..1367)
                     /locus_tag="ACF3T5_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFD3633417.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            1495..2064
                     /locus_tag="ACF3T5_002938"
     CDS             1495..2064
                     /locus_tag="ACF3T5_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFD3633418.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            2131..2868
                     /locus_tag="ACF3T5_002939"
     CDS             2131..2868
                     /locus_tag="ACF3T5_002939"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFD3633419.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            3099..4367
                     /locus_tag="ACF3T5_002940"
     CDS             3099..4367
                     /locus_tag="ACF3T5_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFD3633420.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            4854..5318
                     /locus_tag="ACF3T5_002941"
     CDS             4854..5318
                     /locus_tag="ACF3T5_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFD3633421.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            5302..5931
                     /locus_tag="ACF3T5_002942"
     CDS             5302..5931
                     /locus_tag="ACF3T5_002942"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFD3633422.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            6285..7763
                     /gene="msr(C)"
                     /locus_tag="ACF3T5_002943"
     CDS             6285..7763
                     /gene="msr(C)"
                     /locus_tag="ACF3T5_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFD3633423.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            7810..8256
                     /locus_tag="ACF3T5_002944"
     CDS             7810..8256
                     /locus_tag="ACF3T5_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFD3633424.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(8455..8970)
                     /locus_tag="ACF3T5_002945"
     CDS             complement(8455..8970)
                     /locus_tag="ACF3T5_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFD3633425.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            9087..10289
                     /locus_tag="ACF3T5_002946"
     CDS             9087..10289
                     /locus_tag="ACF3T5_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFD3633426.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         3288 a         1824 c         2122 g         3100 t
ORIGIN      
        1 tggttccatg acaaatatcc gttcgacatc acgatcaaaa atcaaatcat ttctttttct
       61 gtcaaatcca atcccttttc caatagctac tttttcctgt ccaccgtcat ctaccaatac
      121 tgcattttga ttaaagatct ttttgatttt catgcgcgca ccctttttga agatttggtt
      181 ttcacgtttg ctagaccagt tgattgcggc caaacaaaaa tagacacgaa aaacctccta
      241 gggaaaccat gttcctcaga gatttttcat gcctagtatg atctagtcac atgaaacgaa
      301 ttattgttaa cgtaatcaat tttatataga cagttatgtt ttgtcaattc tttttttata
      361 attatagtaa tttactggaa ttatcagaaa taaaaggaag gaacaaatta agaaacttat
      421 gttatttaat cattggatct tttgcttaac ttattcgttg ttaaaaagct gaaagcgaag
      481 gaaaaaaact ctacttaact gtatttctcc gttaggacat ctaaaaattt acgagcagca
      541 ggagaaagag gctggtcttt tttccaaatg agattcagtg aagcttgtaa tggcggatcg
      601 agtgggatga atactttttc cgtcccataa gtattgatga tcccatctag agctaatgca
      661 ccaaaagtcc cgttatcgac catcaaagaa gcattgtaca ataagttata tgttccgacg
      721 atcgataact gatctatgct gaaacctagc cattcagcta gttgactgtc cactgacgtt
      781 tgccgggaaa tgatcaaagg aacagttttc agttcctgcg gatggatgac tgtttgcttt
      841 gctaaaggat gattccgatg tatcaaaagt ccccaacgat cttgggctgg cagtcgaaat
      901 gactggtatt tttgtttgtt tgtcggttcg attacgagtc caaaatcaag taatccgcta
      961 tccagtttgt ctatgatatc atctgcattc ccgctatata aatgaaactg gatatcagga
     1021 tgcgccttga tcaacgaatg aaggctatca gaaatgaaag agattgcttt agtttcgcct
     1081 ccgccaatgt agatttctcc agagatgatt gcgtcatttg ttaaattgtt cgccgttttt
     1141 tctaccaacg acaagatttc ttttcctttt gaataaagat atcgaccaga atccgtcaac
     1201 tgtatctctc gggagcctcg agtgaaaagt gtcacaccaa gttcttcttc taaatctttc
     1261 agttgttttg agagagtagg ttgcgtgata tgcagagatt ttgctgctcg gctgatattt
     1321 ttttctctag ctactgctaa aaaatacgtc agtatacgaa tatccatttt ttccctcctg
     1381 tttttaaaaa agcataccac taagtatagt taaaaggaat agttaagtat gttttataag
     1441 tattagacat cataagccgt ccgttataaa gtaaggataa aaggagggag caaaatgaca
     1501 agcctcatcg aaaaacaaat cgtcaataaa gagattcgag taacggatcc gctctttgaa
     1561 gtgatccatc agatccaagc ggaaaacgaa gagcctttag cagcgctgaa tactgggtac
     1621 catgaaccag cagatatccg caaacggcta gaaaatatca ttagtgacaa agtagatgat
     1681 acggtgactg ttctgctgcc tttttataca gatttcggca aacatattag tatagggaaa
     1741 aatgtcttta tcaatcgtca agtgatgttc gttgacttag gtggcatctg tttagaagat
     1801 tcagtattga ttggtcctcg tgtcaacctc atcacggtga accatctaac cgatccaaaa
     1861 gaacgaagag gcttgtcagt aaagccaatc cacatcaaga aaaatgcttg gatcggagca
     1921 ggagcaacta ttctgccagg cgtaacgatt ggcgagaatg ccattgttgc agcgaatgca
     1981 actgttacga aagatgtccc agacaatacc atcgttgcag gaatccctgc caaaatcgtc
     2041 aaacctgtcg aacggattat ttgaacagta agatgaaata agagatagtg agtataagca
     2101 agagattttt caaaacagga ggagaataga atgaatgatt tatcagaaaa agtgattgtc
     2161 atcatgggcg cttcaagcgg aatcggtgaa gcaacaacga agctattagc agaaaaaggg
     2221 gctaaactag ttatagctgc acgccgagaa gatcgcctaa aagcaataaa agaatcttta
     2281 ccagaagctg agctatatat tcaaacagca gatgtaagag attttgcaca agtccaagca
     2341 gttatcgatt tagcgatgga aaagttcggt cgaatcgatg tattgtacaa taatgcagga
     2401 atcatgccaa ccgctccatt agttgaaggt caccgtgatg aatggcaaaa tatgcttgat
     2461 atcaacatca tgggtgtgtt aaatggaatt tccgccgtac ttccaatcat ggaaaaacaa
     2521 aaatctgggc atatcatttc aactgattct gtggcgggac atgttgttta tcctgattcc
     2581 gccgtttatt gcggaacaaa atttgctgtc cgtgcaatca tggaaggatt acgtcaagag
     2641 caacggcaaa ataacatcaa atctacgatc atctctccag gagctgttca aactgaatta
     2701 tatcaaacca tcagcgacaa aaaggctgct cttgaacttc atgaagcgca aaaagaatgg
     2761 ggattaacat ctgaagatat agcttctgct gtagcatttg cgatagaaac gccagaccgg
     2821 atgtctgtca gcaacatgat tatccgtccg acgacacagg aagtataatc cataacggat
     2881 aatatccaga gagaattact tgttaaatag ttattcaaac gaccatcaat ttaaggcgtg
     2941 aatggagaag aatattcttc atttacgtct ttttatgcag aaaaagcaaa tttattaaaa
     3001 agaaacaaat gttatttaaa tatggacatt gacttgcttt tactttttta gttacactgt
     3061 tatttaggaa aagtttcata attcaaagga gaaaaaagat gaaagcaaag aatttattat
     3121 ttagcgttat tcttttagga aatctagcag ttgccaacac aacagtgcta gctgaagaat
     3181 cgacagaaac tgtcgcaact cagacatcga gttcttcaga aacaggaatt gaaaacgata
     3241 cagaaagtac tttgcagtca gctactgtct cagaaaatac aatttcaacc gaagaaccaa
     3301 caactgaaac aacagaacaa acacaaaccg aagacagtac atcagcagaa gctcagaaca
     3361 atccagagaa tgcagaagaa atcacgaacc aattgacaga tgggcaaaca ttgacacata
     3421 cccatcaagc agatttgtct gaattcgaaa actatactgg ctatgctttt tatgctcgct
     3481 catctgctaa ttctcaacaa gcgttcattg atagtattgc ctcaactgca caaagtttgg
     3541 caagtgccaa tgacttatat gcatcagtca tgattgctca agccatcgtt gaaagtggct
     3601 gggggaacag tactttggct tctgccccta attacaatct atttgggatt aaaggaagct
     3661 ataatggcca atctgttacg atgcctactt cggaatatgt gaatgggcaa tggattacgg
     3721 tgaatgccgc ttttagaaaa tatccttcgt acaaagaatc attgcaagat aacgtaacgg
     3781 tattgaagac gacttctttt cagccaggcg tttactacta ttccggtgca tggaaaagca
     3841 atacgaactc ttacaaagat gctacggctt ggctaacagg tcgttatgcc actgcgccta
     3901 attatggttc tacattgaac aatgtaatcg aaacctataa tctgacacag tatgatacag
     3961 caccaacttc aacttctccg atgtatcgtc tgtataaccg tcatacagga gaacatttat
     4021 atacattgaa tgccggagaa aaaaattatc tgccaactgt aggctgggaa tatgaaggga
     4081 ttgcttggca agcaccaaac agcggacaac ctgtctaccg tttatacaat ccttacagtg
     4141 gagaccatca ttacacaatg gcacaatctg agatcaactc tttaacaaaa atcggttggc
     4201 gttatgaagg tctaagtttt tattctggtg gttccaagcc aatttatcga ctatttaatc
     4261 caaatgaaaa aactggtacc catcactata ccttgagtgc taaggaaaga gattggctaa
     4321 caccgatggg ctggagatat gaaggaatag gattttatgg ttattaaaaa gaggtagtga
     4381 aaaagctgcc ctgcatcaag tagtaagaac agccaaacaa aaacagattt tcatgttttt
     4441 tgtttggttg aacttattac cgcagatgca agtttttgaa caccgtttac tcaaaaaagg
     4501 ttgtgacaac atcttgtcat aactttttta ttactgttta agcatactga tccaatttct
     4561 aatattcgac gggattcttg caaaaggcga tttcggaaat ttttggcggt gtaaaattat
     4621 ctactgcaga agagctatct tctatagttt gataagaatc aataatcttg gttttagcgt
     4681 tgtgctttct ttatcatttt ttccgttcat tctacaaaaa atttcttttc tctatacttt
     4741 tctattgaga gcgttttttt tattacacag ctgattcttt gacttttttc tttttttatt
     4801 ctattattga caagtcaatc gttgacgagt caattattag aaaggaggaa attatgtctt
     4861 tagaagagat tagtcacctt ttatatcaaa tcaaattagc cgatcaaaaa atcgtccaga
     4921 tctttgaaga aaatatcggt ataagtctta ctagatacga gatcatgatg agactgaaag
     4981 aagaaaatcc attgctacaa agcagtttgc aagaaaagtt gcagattgat caaggagcag
     5041 ttactcgtca tctgaaggta ttggaagcaa atggctatgt ttcgcgtcag agaaatcctc
     5101 agaacaaccg ggaagtgtat gtagcgctaa cagaaaaagc ccagaagaaa atcaagcatt
     5161 gtgaaatgga ccataaagat acaggttccg tgctatcccc ggctttcagc gatgtagaaa
     5221 tgaaacaatt tctaacactt ttagataaat tgaataccca actttgcaat ataacaacag
     5281 aaacgaaaga ggttaataat aatggaacaa aaaaatgatt tttcaacaat tatgactggt
     5341 agaagatccg ttcgcgtata tgatgaaaca tacaaaatcc cacacgaaga aatgctggaa
     5401 atgatcaaag aagcgacgac tgcaccttct tctgtcaata tgaaaccatg gcgctttgtg
     5461 gtcgctgaaa gcgaggaagc aaaagaaatc ttgcgtcctt tgatccgttt caatacgaga
     5521 caaaatgata cttcttctgc aatggtcatg atttttggcg atatgcaatg ttacgaatat
     5581 ggcgaagaaa tctacaacca agcttacgaa tcagggaaga tgccaaaaga agtcagagat
     5641 caacaattag ctgcgatcat tccttattac aagagttttt caagagaaga aatgaacgat
     5701 gtagtcaaag tcgattctag cttagcggct atgcaattca tgttagttgc gcgtgatcat
     5761 ggctatgaaa caaatccaat tggtggattc gaagcagatc agttggcaga ggcatttggt
     5821 ttggataaag accgttatgt tccagttatt attttatcaa taggaaaagc ggtagaagaa
     5881 ggttacgagt ctgttcgttt agcaccagaa aagatcacta tgttcgaata aaaggttttc
     5941 agttactatt cagtagctat tgcttttttt gaatcgtgtg tggtaaccta ataaaaaagg
     6001 aggtcggaaa aatgactgca tcgatgaaat tacgtttcga acttttgaat aacaactaaa
     6061 taaattcaaa agacatgtat aaacaacaat ttcatggatg gagtatgtcc attttttcga
     6121 acgacagtag aataggtgaa agctatgaca gatttggtga tttagtcata gttatttcgt
     6181 tcagagtagg aaaagatgtg atgcagtttt tttgcaaaca gatttttctg ctctttttta
     6241 gtatcgataa ttgttgtttt acatgaaata ggagtgtaaa acatatggaa aatttagcag
     6301 taaatataac aaatctacaa gtcagctttg gcaaccagct agaactatct attgattctc
     6361 ttcgtgtcta tcagcaagat cgtataggga tcataggaga aaatggtgtc ggtaaatcga
     6421 ctttgctcaa actaatagcc ggtgaacttt ttcccgatca tgggaaaatc caaacagaga
     6481 tcaccttcaa ctacctgcct caattaacct atctcgctga agcaaaggac ctaaatttgg
     6541 aattagccag tcatttccag ttaagactgg aagaaacttc agagcggaaa tggagcggag
     6601 gggaagaacg aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc
     6661 tagacgagcc aacaacccat ctagatagaa aaagtattga tcgactgatt gaagagcttc
     6721 gttattatta tggtacgctg gtttttgtta gtcatgatcg ctattttcta gatgagctag
     6781 catcgaaaat ctgggaagta aaagacggag aaatccgaga gttttcgggg aattatagtg
     6841 cctatctcac tcaaaaggaa ttggagaaaa agactcagct acgagaagca gagtcgatca
     6901 tgaaagagaa aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt
     6961 tggaaaaagt gtccagtaaa aagaaaaagc aacaaatcag actggatcgg ttgtcttcct
     7021 ctaaacaaaa agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa
     7081 gactccaaaa aataggagaa acaaccaagc cgcagcagat gaaacaaatc cgttttccag
     7141 taccaaaatc tcttgaactc cacagccgct atcccatcat gggacaaaat gtccaattgg
     7201 aaagaagtgg gagaacatta ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa
     7261 tcgcgattgt cggtgaaaat ggttcaggta agacaacctt attagaacat atccgcaaac
     7321 aaggagaagg aatccttctc tctccgaaag taagctttca agtatatcag caaaagggtt
     7381 atcaaatgac atctgaagaa tccatcattc gttttgtcat gagacaaaca gagttttcag
     7441 aatcgcttgt ccgtagtttg ctgaatcact tagggtttgc tcaggaaact ttgacgaaac
     7501 cgttatgtac attaagtggg ggagaagcga cccgtctgac gattgctttg ctttttacta
     7561 agccaagtaa tgtgttgctg ttagatgaac cgactaattt tattgatatg gcaacgatcg
     7621 aagctttaga gaagctaatg caaatatatc cgggaacgat tttgtttact tctcatgatt
     7681 cttactttgt cgagcgtacg gctgatgaag tttatgaaat aaaagggcag aaaataaaaa
     7741 aagtacttac gagaaatttt taaatgtgct acgatgattt tcgtgacaaa tttaaaggag
     7801 tggtacagat tgcctacaaa taaaaaagaa ggaataattt ttacaacaat gatgtgtttt
     7861 ttgatggtct tggggatgag cgcgtataat ttatggctgc atcaagatct tcatttatca
     7921 agtttgttga ttggtctagt gcctggattc gtggtggctt tcattttgga tgtcttcatc
     7981 atcggtgtgc tggcgaaaaa aattgccttt aaactaccag taaatccaga aagcaaggtc
     8041 caaatgatct taactatttc atgcttgatg gttttaggaa tggtgacatg tatgtcttta
     8101 ttcggtgtga tcatggaaca aggaatacct gatcaattat tctctgctta tatgaatgct
     8161 tggaaaatga atgtgattgc cgctttaccg ttgcagttga tcatcgttgg cccgctttca
     8221 agaagtgtgc taaaagcaat tcaaagtaat gaatgatgga acactgttat ttgaaaaaag
     8281 gccttggcat gctttttgtc atagtctctt gaatgcagtt ctagaaaaat agtgatccga
     8341 acgataggtt ttggcctgtc gttcggatcg ctttttcttt tccaactgta ttgctctttt
     8401 attgaagata atttgctctt caatagatat ttctagaaag gttctgttta tcccttactc
     8461 aaaaaactgc tgcatttgtt ctcttacttg ctgacaccat tggagttcct gctgacaaag
     8521 cataattctg cgctggatca caacataacg tccaaattct tgtttcttgt ctagcgagag
     8581 gtcattcaat tttttttgat ttttttgcat gattttgttc agctgttgtt ctctagtgaa
     8641 tagcagtcca gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt ttgctagaaa
     8701 ttcatctttt tgcgtaggag cgggagtttc ctctttgatc cattcctcta caagagcacg
     8761 tccttcatca gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt ccaaaacggt
     8821 cacataaccc tgctggtcta acttggcaag cgttgtatag atctgactgt gatgggcttc
     8881 ccaaaaaatt gtgatcagct gcttcaattc atacccagaa cgtggtttgg ataaaagcac
     8941 gcataataat atataggcta aagtattcat aaaaatccta cctctcgtct tattttcttt
     9001 attatatcac agtaaagttt ccacgtttta catgtgcata ttgacatgta aaataaatca
     9061 tgtatgatgc taatagagag aaagggatgg aaatattgga gaatcaaata gatatacaag
     9121 aacaaacaaa tgaagaaaca aaggggaaaa gttcagcgct agttgtagca gctacctgta
     9181 tgatcgcttt tatgggagtg ggtctagtag acccaatctt aaaaacgatt gctgcacagt
     9241 taaacgctac gccagctgaa acgaccctgt tatttactag ttacatgtta gtcactggtg
     9301 tggtgatgct atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa acattgatga
     9361 caggattgtt tatcatcgtt gtattcgctg gtcttggcgg attatctggg aatatcggaa
     9421 tgctgattgg gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc tctactgctt
     9481 tagcaacgat cgtcagtatt ttagttggta acacagaaaa agcaatcatg atgtatgaag
     9541 cagcacttgg ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg ggcagtggtt
     9601 catggcgtta tccatttttt ggggtagcat ttttgatgtt ttgcggtttt atagccctag
     9661 cggtactttt acctgaaact gagaaaccaa agagaaaagt aggcctttgg gaaggtgtca
     9721 aagcgttaag caatcataaa cttcgttcag ttggactaat cgcattgtta tataatttcg
     9781 gattctttac tttacttgcg tatgcgccat ttttattgat tggctattct gaattagaag
     9841 tcggttttgt attttttggc tggggtgtgc ttctggctat tgcttcgatt ttcgttgcac
     9901 cggctcttga gcggaagttt acgacttatc ggacaatttt agcagcattt gtcttattca
     9961 ttatttgttt gctgttatta ggagctggaa cttctatgcc agtccttgta ccgatcagca
    10021 ttgtgttagc aggatttttc caaggaatta tcaatacact gttgacgacg attgctatgg
    10081 aaataccagg tctgcaacga aacgttgctt cgtccagcta tagttttgtc cgctttttcg
    10141 gtggagcttt agcaccattt attgcaggtg tgattggtga acatattggt gaaagctatg
    10201 cgttttattt tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt tatcaccgta
    10261 attattttgc tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa gctgtcctgc
    10321 atcaagtcat aaga
//
LOCUS       DAXLPI010000362         7359 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.116, whole genome shotgun sequence.
ACCESSION   DAXLPI010000362 DAXLPI010000000
VERSION     DAXLPI010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.116"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            104..799
                     /gene="vanR-A"
                     /locus_tag="ACF3T5_002947"
     CDS             104..799
                     /gene="vanR-A"
                     /locus_tag="ACF3T5_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-A"
                     /protein_id="HFD3633427.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            777..1931
                     /locus_tag="ACF3T5_002948"
     CDS             777..1931
                     /locus_tag="ACF3T5_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-A"
                     /protein_id="HFD3633428.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            2146..3114
                     /locus_tag="ACF3T5_002949"
     CDS             2146..3114
                     /locus_tag="ACF3T5_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HFD3633429.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            3107..4138
                     /gene="vanA"
                     /locus_tag="ACF3T5_002950"
     CDS             3107..4138
                     /gene="vanA"
                     /locus_tag="ACF3T5_002950"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HFD3633430.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            4144..4752
                     /locus_tag="ACF3T5_002951"
     CDS             4144..4752
                     /locus_tag="ACF3T5_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HFD3633431.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            5202..6092
                     /locus_tag="ACF3T5_002952"
     CDS             5202..6092
                     /locus_tag="ACF3T5_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HFD3633432.1"
                     /translation="MLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLENSGTSE
                     NTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMS
                     KEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGL
                     SLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHS
                     AIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGN
                     NIDGVIVTVFPGSTHTNSRR"
     gene            6245..6730
                     /gene="vanZ-A"
                     /locus_tag="ACF3T5_002953"
     CDS             6245..6730
                     /gene="vanZ-A"
                     /locus_tag="ACF3T5_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HFD3633433.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            7000..7230
                     /locus_tag="ACF3T5_002954"
     CDS             7000..7230
                     /locus_tag="ACF3T5_002954"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633434.1"
                     /translation="MNKVHVYNHNKFVKEINSDTNSIAKEIASQSPFAELKMTDEYDN
                     LILTTKGFFLDDVPNQIWLKNELMPALTNLQI"
BASE COUNT         2390 a         1282 c         1607 g         2080 t
ORIGIN      
        1 taaaatttgg tagtttataa aaagtatgaa ttattcaggt ataaattcgg ccctataatc
       61 tgataaatta ttaagggcaa acttatgtga aagggtgata actatgagcg ataaaatact
      121 tattgtggat gatgaacatg aaattgccga tttggttgaa ttatacttaa aaaacgagaa
      181 ttatacggtt ttcaaatact ataccgccaa agaagcattg gaatgtatag acaagtctga
      241 gattgacctt gccatattgg acatcatgct tcccggcaca agcggcctta ctatctgtca
      301 aaaaataagg gacaagcaca cctatccgat tatcatgctg accgggaaag atacagaggt
      361 agataaaatt acagggttaa caatcggcgc ggatgattat ataacgaagc cctttcgccc
      421 actggagtta attgctcggg taaaggccca gttgcgccga tacaaaaaat tcagtggagt
      481 aaaggagcag aacgaaaatg ttatcgtcca ctccggcctt gtcattaatg ttaacaccca
      541 tgagtgttat ctgaacgaga agcagttatc ccttactccc accgagtttt caatactgcg
      601 aatcctctgt gaaaacaagg ggaatgtggt tagctccgag ctgctatttc atgagatatg
      661 gggcgacgaa tatttcagca agagcaacaa caccatcacc gtgcatatcc ggcatttgcg
      721 cgaaaaaatg aacgacacca ttgataatcc gaaatatata aaaacggtat ggggggttgg
      781 ttataaaatt gaaaaataaa aaaaacgact attccaaact agaacgaaaa ctttacatgt
      841 atatcgttgc aattgttgtg gtagcaattg tattcgtgtt gtatattcgt tcaatgatcc
      901 gagggaaact tggggattgg atcttaagta ttttggaaaa caaatatgac ttaaatcacc
      961 tggacgcgat gaaattatat caatattcca tacggaacaa tatagatatc tttatttatg
     1021 tggcgattgt cattagtatt cttattctat gtcgcgtcat gctttcaaaa ttcgcaaaat
     1081 actttgacga gataaatacc ggcattgatg tacttattca gaacgaagat aaacaaattg
     1141 agctttctgc ggaaatggat gttatggaac aaaagctcaa cacattaaaa cggactctgg
     1201 aaaagcgaga gcaggatgca aagctggccg aacaaagaaa aaatgacgtt gttatgtact
     1261 tggcgcacga tattaaaacg ccccttacat ccattatcgg ttatttgagc ctgcttgacg
     1321 aggctccaga catgccggta gatcaaaagg caaagtatgt gcatatcacg ttggacaaag
     1381 cgtatcgact cgaacagcta atcgacgagt tttttgagat tacacggtat aacctacaaa
     1441 cgataacgct aacaaaaacg cacatagacc tatactatat gctggtgcag atgaccgatg
     1501 aattttatcc tcagctttcc gcacatggaa aacaggcggt tattcacgcc cccgaggatc
     1561 tgaccgtgtc cggcgaccct gataaactcg cgagagtctt taacaacatt ttgaaaaacg
     1621 ccgctgcata cagtgaggat aacagcatca ttgacattac cgcgggcctc tccggggatg
     1681 tggtgtcaat cgaattcaag aacactggaa gcatcccaaa agataagcta gctgccatat
     1741 ttgaaaagtt ctataggctg gacaatgctc gttcttccga tacgggtggc gcgggacttg
     1801 gattggcgat tgcaaaagaa attattgttc agcatggagg gcagatttac gcggaaagca
     1861 atgataacta tacgacgttt agggtagagc ttccagcgat gccagacttg gttgataaaa
     1921 ggaggtccta agagatgtat ataatttttt aggaaaatct caaggttatc tttacttttt
     1981 cttaggaaat taacaattta atattaagaa acggctcgtt cttacacggt agacttaata
     2041 ccgtaagaac gagccgtttt cgttcttcag agaaagattt gacaagatta ccattggcat
     2101 ccccgtttta tttggtgcct ttcacagaaa gggttggtct taattatgaa taacatcggc
     2161 attactgttt atggatgtga gcaggatgag gcagatgcat tccatgctct ttcgcctcgc
     2221 tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg
     2281 cctttcaatc aatgtatcag tgtgggacat aaatcagaga tttccgcctc tattcttctt
     2341 gcgctgaaga gagccggtgt gaaatatatt tctacccgaa gcatcggctg caatcatata
     2401 gatacaactg ctgctaagag aatgggcatc actgtcgaca atgtggcgta ctcgccggat
     2461 agcgttgccg attatactat gatgctaatt cttatggcag tacgcaacgt aaaatcgatt
     2521 gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg accgtggcaa ggtactcagc
     2581 gacatgacag ttggtgtggt gggaacgggc cagataggca aagcggttat tgagcggctg
     2641 cgaggatttg gatgtaaagt gttggcttat agtcgcagcc gaagtataga ggtaaactat
     2701 gtaccgtttg atgagttgct gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat
     2761 acggatacgc actatattat cagccacgaa caaatacaga gaatgaagca aggagcattt
     2821 cttatcaata ctgggcgcgg tccacttgta gatacctatg agttggttaa agcattagaa
     2881 aacgggaaac tgggcggtgc cgcattggat gtattggaag gagaggaaga gtttttctac
     2941 tctgattgca cccaaaaacc aattgataat caatttttac ttaaacttca aagaatgcct
     3001 aacgtgataa tcacaccgca tacggcctat tataccgagc aagcgttgcg tgataccgtt
     3061 gaaaaaacca ttaaaaactg tttggatttt gaaaggagac aggagcatga atagaataaa
     3121 agttgcaata ctgtttgggg gttgctcaga ggagcatgac gtatcggtaa aatctgcaat
     3181 agagatagcc gctaacatta ataaagaaaa atacgagccg ttatacattg gaattacgaa
     3241 atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta
     3301 ttcagctgta ctctcgccgg ataaaaaaat gcacggatta cttgttaaaa agaaccatga
     3361 atatgaaatc aaccatgttg atgtagcatt ttcagctttg catggcaagt caggtgaaga
     3421 tggatccata caaggtctgt ttgaattgtc cggtatccct tttgtaggct gcgatattca
     3481 aagctcagca atttgtatgg acaaatcgtt gacatacatc gttgcgaaaa atgctgggat
     3541 agctactccc gccttttggg ttattaataa agatgatagg ccggtggcag ctacgtttac
     3601 ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa
     3661 tagcgcggac gaattggact acgcaattga atcggcaaga caatatgaca gcaaaatctt
     3721 aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc
     3781 gttagttgtt ggcgaggtgg accaaatcag gctgcagtac ggaatctttc gtattcatca
     3841 ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata accgttcccg cagacctttc
     3901 agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa atatataaag cgctcggctg
     3961 tagaggtcta gcccgtgtgg atatgttttt acaagataac ggccgcattg tactgaacga
     4021 agtcaatact ctgcccggtt tcacgtcata cagtcgttat ccccgtatga tggccgctgc
     4081 aggtattgca cttcccgaac tgattgaccg cttgatcgta ttagcgttaa aggggtgata
     4141 agcatggaaa taggatttac ttttttagat gaaatagtac acggtgttcg ttgggacgct
     4201 aaatatgcca cttgggataa tttcaccgga aaaccggttg acggttatga agtaaatcgc
     4261 attgtaggga catacgagtt ggctgaatcg cttttgaagg caaaagaact ggctgctacc
     4321 caagggtacg gattgcttct atgggacggt taccgtccta agcgtgctgt aaactgtttt
     4381 atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg aaagttatta tcccaatatt
     4441 gaccgaactg agatgatttc aaaaggatac gtggcttcaa aatcaagcca tagccgcggc
     4501 agtgccattg atcttacgct ttatcgatta gacacgggtg agcttgtacc aatggggagc
     4561 cgatttgatt ttatggatga acgctctcat catgcggcaa atggaatatc atgcaatgaa
     4621 gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca gtgggtttga agcatatagc
     4681 ctcgaatggt ggcactatgt attaagagac gaaccatacc ccaatagcta ttttgatttc
     4741 cccgttaaat aaacttttaa ccgttgcacg gacaaactat ataagctaac tctttcggca
     4801 ggaaacccga cgtatgtaac tggttcttag ggaatttata tatagtagat agtattgaag
     4861 atgtaaggca gagcgatatt gcggtcatta tctgcgtgcg ctgcggcaag atagcctgat
     4921 aataagactg atcgcataga ggggtggtat ttcacaccgc ccattgtcaa caggcagttc
     4981 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
     5041 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
     5101 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
     5161 gttaaggagg agactgaaaa tgaagaagtt gttttttttt attgttattg ttattcttaa
     5221 tatacttagg ttatgactac gttaatgaag cactgttttc tcaggaaaaa gtcgaatttc
     5281 aaaattatga tcaaaatccc aaagaacatt tagaaaatag tgggacttct gaaaataccc
     5341 aagagaaaac aattacagaa gaacaggttt atcaaggaaa tctgctatta atcaatagta
     5401 aatatcctgt tcgccaagaa agtgtgaagt cagatatcgt gaatttatct aaacatgacg
     5461 aattaataaa tggatacggg ttgcttgata gtaatattta tatgtcaaaa gaaatagcac
     5521 aaaaattttc agagatggtc aatgatgctg taaagggtgg cgttagtcat tttattatta
     5581 atagtggcta tcgagacttt gatgagcaaa gtgtgcttta ccaagaaatg ggggctgagt
     5641 atgccttacc agcaggttat agtgagcata attcaggttt atcactagat gtaggatcaa
     5701 gcttgacgaa aatggaacga gcccctgaag gaaagtggat agaagaaaat gcttggaaat
     5761 acgggttcat tttacgttat ccagaggaca aaacagagtt aacaggaatt caatatgaac
     5821 catggcatat tcgctatgtt ggtttaccac atagtgcgat tatgaaagaa aagaatttcg
     5881 ttctcgagga atatatggat tacctaaaag aagaaaaaac catttctgtt agtgtaaatg
     5941 gggaaaaata tgagatcttt tattatcctg ttactaaaaa taccaccatt catgtgccga
     6001 ctaatcttcg ttatgagata tcaggaaaca atatagacgg tgtaattgtg acagtgtttc
     6061 ccggatcaac acatactaat tcaaggaggt aaggatggcg gaatgaaacc aacgaaatta
     6121 atgaacagca ttattgtact agcacttttg gggtaacgtt agctttttaa tttaaaaccc
     6181 acgttaacta ggacattgct atactaatga tacaacttaa acaaaagaat tagaggaaat
     6241 tatattggga aaaatattat ctagaggatt gctagcttta tatttagtga cactaatctg
     6301 gttagtgtta ttcaaattac aatacaatat tttatcagta tttaattatc atcaaagaag
     6361 tcttaacttg actccattta ctgctactgg gaatttcaga gagatgatag ataatgttat
     6421 aatctttatt ccatttggct tgcttttgaa tgtcaatttt aaagaaatcg gatttttacc
     6481 taagtttgct tttgtactgg ttttaagtct tacttttgaa ataattcaat ttatcttcgc
     6541 tattggagcg acagacataa cagatgtaat tacaaatact gttggaggct ttcttggact
     6601 gaaattatat ggtttaagca ataagcatat gaatcaaaaa aaattagaca gagttattat
     6661 ttttgtaggt atacttttgc tcgtattatt gctcgtttac cgtacccatt taagaataaa
     6721 ttacgtgtaa gatgtctaaa tcaagcaatc tgatctttca tacacataaa gatattgaat
     6781 gaattggatt agatggaaaa cgggatgtgg ggaaactcgc ccgtaggtgt gaagtgaggg
     6841 gaaaaccggt gataaagtaa aaagcttacc taacactata gtaacaaaga aagcccaatt
     6901 atcaatttta gtgctgagga attggtctct ttaataaatt tccttaacgt tgtaaatccg
     6961 cattttcctg acggtacccc ataaaggaga ataaataaaa tgaataaggt acatgtatat
     7021 aatcataata aatttgtaaa agaaattaat agcgacacga atagcattgc taaagaaatt
     7081 gctagtcaat ccccatttgc tgaattaaaa atgacagatg aatacgataa cttaatttta
     7141 acaacaaaag gctttttcct agatgatgta cctaatcaga tatggctaaa aaatgagtta
     7201 atgccagctc ttacaaattt acagatttaa aaaataacct cacctgatca cattagtata
     7261 attaggtgag gttattttat atattgcgtt gaatattatg tttaatccta tatcttctaa
     7321 atgtttcatg attaatacca gttaaatttt gaacctctt
//
LOCUS       DAXLPI010000363         6169 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.130, whole genome shotgun sequence.
ACCESSION   DAXLPI010000363 DAXLPI010000000
VERSION     DAXLPI010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.130"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..2410
                     /locus_tag="ACF3T5_002955"
     CDS             <1..2410
                     /locus_tag="ACF3T5_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFD3633435.1"
                     /translation="TKKTKKQKQQQEQMLTIGVGFALILFAVFGFLKLGFLGILIANG
                     FRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFYFGILLLLHAHLFG
                     KLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYFLIAQPGSYLAAVL
                     LLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAAKEERMKQRAEAKE
                     ARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPIDSFQENIH
                     PANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPTVDLLDSIP
                     TVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVGVKVSKIVN
                     LTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNHPDKLLEVP
                     LGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEVKLMMIDPK
                     MVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNISGYNEFVQ
                     QKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGIHMILATQR
                     PSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLPMGENKPIR
                     VQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELFEEAKALVV
                     EMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQEETSETLPD
                     DNDM"
     gene            2640..3707
                     /gene="rlmN"
                     /locus_tag="ACF3T5_002956"
     CDS             2640..3707
                     /gene="rlmN"
                     /locus_tag="ACF3T5_002956"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFD3633436.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            3697..3903
                     /locus_tag="ACF3T5_002957"
     CDS             3697..3903
                     /locus_tag="ACF3T5_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633437.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(3900..5003)
                     /locus_tag="ACF3T5_002958"
     CDS             complement(3900..5003)
                     /locus_tag="ACF3T5_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFD3633438.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5556..6104
                     /gene="aac(6')"
                     /locus_tag="ACF3T5_002959"
     CDS             5556..6104
                     /gene="aac(6')"
                     /locus_tag="ACF3T5_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFD3633439.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2044 a         1066 c         1332 g         1727 t
ORIGIN      
        1 gacgaaaaaa acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg
       61 gtttgccttg attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt
      121 gattgcaaac ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt
      181 agccgtatta ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg
      241 acgctggttt ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt
      301 atttggcaaa ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc
      361 cagtgatata aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat
      421 cctttatcat tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct
      481 attactggca ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg
      541 tatccaatca attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc
      601 tcgaaagcaa gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag
      661 gcgcttagct gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc
      721 ggtgagccag caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc
      781 ggagcagcta agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct
      841 agagaaacca gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga
      901 gttgccagag gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca
      961 agactatgaa ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga
     1021 tgagtataaa aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg
     1081 tgtcgatgcc aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca
     1141 gccggctgta ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc
     1201 tttagcggcg aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat
     1261 tgaagtacca aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc
     1321 aaaccaccca gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca
     1381 aacggctgat ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa
     1441 gtcagtagca attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt
     1501 aaaactgatg atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca
     1561 tctgttgacg ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt
     1621 acaagaaatg gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg
     1681 ttacaatgaa tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc
     1741 attcatcgta gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt
     1801 tgaagatgcg attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct
     1861 agctactcaa cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc
     1921 cagaatggca tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg
     1981 ggcagaaaag ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc
     2041 gatccgtgtg caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt
     2101 tacagatcag caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac
     2161 gacaggtgct ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt
     2221 tgaaatgcaa acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg
     2281 agcagcacgt ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag
     2341 caagcctcgg aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa
     2401 tgacatgtaa aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg
     2461 catgatgata ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc
     2521 ttgtcttttt agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat
     2581 taaggaaatg aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt
     2641 tggaaaaaca gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa
     2701 acggcgagaa aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa
     2761 gtaatttcac agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt
     2821 ttatcatcaa tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat
     2881 atttgtttga actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg
     2941 gcttgtcagt ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa
     3001 gcgggctgct caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga
     3061 tggtccaaca ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa
     3121 tggggatcgg tgagccattt gataattacg acaatgtaat ggactttttg catatcatca
     3181 atgatgccaa aggattggca attggtgctc ggcacattac agtttccact agtggattag
     3241 cacacaaaat caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc
     3301 acgcgccgaa taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg
     3361 agaaattgat ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg
     3421 aatatatcat gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc
     3481 tattaaaaga taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg
     3541 aacatgatca atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga
     3601 agaaaaacgg cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt
     3661 gcggacaatt gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc
     3721 gttttttcaa ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat
     3781 aagagaaaag attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc
     3841 aagcaaagaa atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt
     3901 taactgattt tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca
     3961 agaatggcct gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt
     4021 gttgccatgg ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg
     4081 gttaacgata ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg
     4141 aactgctgat ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg
     4201 tatccctcca ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa
     4261 ttatagttat tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg
     4321 acttgttcaa ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca
     4381 taagtcaaga gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag
     4441 cctagatcat cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca
     4501 atacctgccc ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg
     4561 tgtacaggtg gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg
     4621 acacataccg cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt
     4681 ttagctagtt tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca
     4741 aacaggacgt ctgctttttg gacactttca tgctggatca actgttcaat atttccataa
     4801 gttgaattac ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact
     4861 tttattttgt tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc
     4921 ccatattctt tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt
     4981 ggtaagaaaa cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa
     5041 ttttaaagag tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag
     5101 gataagtgat gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac
     5161 gaacaagtac aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct
     5221 accgattccc tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat
     5281 gaaaacaggt tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat
     5341 atcatgatga acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt
     5401 ttgaataaag tgtttctgta taattttttt attttataag taagataaat aatgaaagaa
     5461 tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg
     5521 actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta
     5581 ataatccagt attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat
     5641 atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag
     5701 cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca
     5761 caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta
     5821 ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt
     5881 taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata
     5941 catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa
     6001 aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata
     6061 tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga
     6121 cagaagtaat cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAXLPI010000364         5854 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.137, whole genome shotgun sequence.
ACCESSION   DAXLPI010000364 DAXLPI010000000
VERSION     DAXLPI010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.137"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            218..1114
                     /locus_tag="ACF3T5_002960"
     CDS             218..1114
                     /locus_tag="ACF3T5_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFD3633440.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1264..2205)
                     /locus_tag="ACF3T5_002961"
     CDS             complement(1264..2205)
                     /locus_tag="ACF3T5_002961"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFD3633441.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2331..3452
                     /locus_tag="ACF3T5_002962"
     CDS             2331..3452
                     /locus_tag="ACF3T5_002962"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFD3633442.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3710..5212
                     /gene="eat(A)"
                     /locus_tag="ACF3T5_002963"
     CDS             3710..5212
                     /gene="eat(A)"
                     /locus_tag="ACF3T5_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFD3633443.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5279..5806
                     /locus_tag="ACF3T5_002964"
     CDS             5279..5806
                     /locus_tag="ACF3T5_002964"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFD3633444.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         1928 a         1032 c         1224 g         1670 t
ORIGIN      
        1 tagcaatatt taacaatgac taatgataac aaattttcag acaattacaa ctattcagaa
       61 aacttttagg cgaattttaa tagaagaatg atttatttca tcatattttc atacaaatgt
      121 taaattttca tacaaatata ggaaacgttt tactggcttt ttactattta aacaggctat
      181 cataggaaaa agtgacagag aaagtgaggc ggaaaagatg aacaaaaatg acgtactgct
      241 agttgcaaaa aataaatcac tttatttttt gccttccatc cgctctttag atgtttggtc
      301 cggaaaagat ttactacagt cggaattcat taaatcagct agacaacagt tggcaaatta
      361 tcagctgatc gtttttttag attatggatt tgatatggag atggctcgac gcgtgcgccc
      421 atttaccaat gcaaaaattg tgttattttt ttggaatcac ttcaaagcag aacacgaggc
      481 gatgctgaag caggcacaag atgaaccggc aatcgatgag atttatcatt ttgatttttt
      541 ggaagcaaaa gaacttgggc tgaaacataa tagttcattt tattcgaaac atatgaattt
      601 ttcttctagc gaaccaaaga cggatctatt ttttggtgca acgaataatg ggagaaaaga
      661 acgagcagaa agctataagc aggaatttat aaaaagagat gttacgacca actatttcat
      721 tcttccacat cgagggaatg aacaacctgg atatcttact tatgaagagt acctgaaaaa
      781 aacaggggaa tcgcgcggga ttctggaact gttgcgggag ggtcagcaag gagtgacttt
      841 gcggacattt gaatccatgt attttcaaaa gaaactagtg acggataatc aagcgattgc
      901 tcattatcat ttttatcgtc ctgaaaatat ttttttgctt cgagaacgtg atttagatga
      961 attaccggaa tttctgcata caccatttaa gccaatccta tcagagctat tggaatttta
     1021 tgatgcagaa aattgggcaa aacgatttat cactaacgat tccactattt atgagcaata
     1081 cgaatatgac ctctcctctg ttgaaaacaa ataaaaaacc tacattttga agagattatg
     1141 tactgaaaca aacaagcaca attcttatgg catcatgaga gttgtgtttt ctttttgatc
     1201 ttttattggg cttaaaaaaa gagaaccagc tgctgattca gctgattctc atcagagagc
     1261 cttttattct gcgccaagga ttccttcttt tgcatggact aattgggtca aaaaggcaca
     1321 atatggtgta gcaatatcat atttttgtcc ttttcgccaa atggcaccat tgatgtagtc
     1381 gatttcagtc aaacgatggt ttttgatcag gtcttggtac atagatgggt aatgcaaccc
     1441 gatgttgtca ggatcataag tagaagcaat atgttggtac acttcttctt gatctagtat
     1501 gattccttct tttgctgcga tagaagcaaa ttcagatacg attgttcgaa ccatgctctc
     1561 tgccgctttt tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc ataaaccatt
     1621 caatgttcca tttacacaag cttttcgcca aatggaataa cgaacgttat ggctatagat
     1681 cggatttaaa tttgcttctt gaaagacttc tacaactttt tttgtaaatg cttcgccttc
     1741 tggttccaag ttctctagtt cgatttctcc atcacctaac aatgtgactt ttccgggacc
     1801 agctagacca gctgtccaca tcgtgatccc aaataagata tttttctttg gcacatattt
     1861 ttccagtaca tcttcatgac ctaatccatt tagtaaacac aaaacataag tattgtcagt
     1921 aataatggat tggatcgaac aaaacatatc atctaattga ttggcttttg ttaatgctac
     1981 gatcaagtcc acgtgttcgt tgctttcgat gatttcttct ggtgaataga ttggcagttt
     2041 tgcgaccact tctttaccgt taaagtcagc aatcaaccca tttgtacgaa tggcttcaat
     2101 gtgagcaggc caacgatcga tcaagaggac ctcatttccg ctttgatgta gcatcaatcc
     2161 gattcggctg cccattgctc cggccccagc tattgcgatt ttcatgtttt ttctctcctt
     2221 tattcaatga gttccctttt agtatataga aaaatggaag aaaatagcat tgttttgttt
     2281 gaaaaaaatg aaaaaaagtg tcttacttaa gaagaaagtg agggatacag atggatattt
     2341 tggcggcaat tgattcattt aaaggaagtg cttcttcatt tgaactaaat caggcagttc
     2401 taaatggctg ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca gatggcggag
     2461 aaaatactgc agaagcaatc tattacgcgc tcaggggaac atggcgtacc attgatggga
     2521 tggatctctt attccgtcag cgaaaaatca gctatttatt gacaacttgg caaggaaaaa
     2581 acaccgcagt catcgaatct gcacaagcat tggggttaga tctacttgat aaaccaacgg
     2641 atcaaactat ccgacttgct tccagttatg ccttaggaga acttattaaa gatgcactgc
     2701 ttcaagaagt agaacaaatt attgtcactt taggaggaag cgggtgtagc gatggtggtc
     2761 taggattact ccaatccttg ggtgcttctt tagaagggta cacagaagga aatcctttat
     2821 tatctactaa aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa gtaaaattga
     2881 ttatcgctgc agatgttacc aatccatata caggtcaaca aggagctgct tggcaatttg
     2941 gaagacaaaa aggaggaata gatacgacgc tagctttctt agataaacaa gcaggaaaga
     3001 ttgctcagca aataaaagat ttctgggagg tggatttaga tgatattcca ggaagtggag
     3061 cagcaggcgg tattggtgga gccctttttt tgttgggagc gaagatggtt tctggtttcg
     3121 atttagtcag ccaaatcact ggattggaaa aaaagatcga acaagcagat gtcatcatta
     3181 caggagaagg gcggatcgat cagcaaacga tccacggaaa agttccatac ggtgtagcag
     3241 tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg cggaagtaga gaccaagagc
     3301 tagaaggact ttctgatttt ctcccaattg tcttaagtat tcatttgggt ccaataggtt
     3361 tagaagaagc aatggaacac aggcaaacat tagaaaaaac aaaaatctta ggacagacac
     3421 ttagcagatt tttcaccatc ctcccgaagt agttgacaaa atcattttct ttatgctatc
     3481 gtaatttcaa atataggaca aagtgaggga tccgtgcatt gaaaaattaa gtcgattgat
     3541 gaaagctgca ttcagactgt accttgtgga gacaaggaag cagtgtgcag attttcggga
     3601 ttggcttggt tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt
     3661 tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat
     3721 cgaaataaaa aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc
     3781 aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa
     3841 gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca
     3901 gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt
     3961 gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca
     4021 aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa
     4081 agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac
     4141 gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca
     4201 aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt
     4261 agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca
     4321 gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag
     4381 taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa
     4441 aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt
     4501 gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac
     4561 ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat
     4621 gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta
     4681 tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat
     4741 ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa
     4801 cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca
     4861 aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta
     4921 ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa
     4981 gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca
     5041 gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga
     5101 acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca
     5161 aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta
     5221 atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat
     5281 gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg
     5341 ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt
     5401 agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga
     5461 tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt
     5521 agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc
     5581 acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg
     5641 gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg
     5701 ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt
     5761 ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt
     5821 tatttaaata aaatatttca aggcactgct tttg
//
LOCUS       DAXLPI010000365         3719 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.168, whole genome shotgun sequence.
ACCESSION   DAXLPI010000365 DAXLPI010000000
VERSION     DAXLPI010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.168"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(530..655)
                     /locus_tag="ACF3T5_002965"
     CDS             complement(530..655)
                     /locus_tag="ACF3T5_002965"
                     /inference="COORDINATES: protein motif:HMM:NF019687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633445.1"
                     /translation="MLFPPFKSSKSRKNPSDKYSYHWDFNTKIAVYYTLIATDAL"
     gene            640..2580
                     /gene="tet(S)"
                     /locus_tag="ACF3T5_002966"
     CDS             640..2580
                     /gene="tet(S)"
                     /locus_tag="ACF3T5_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HFD3633446.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINLH"
     gene            2668..2835
                     /locus_tag="ACF3T5_002967"
     CDS             2668..2835
                     /locus_tag="ACF3T5_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HFD3633447.1"
                     /translation="MCPICGNKTRLKMREDTELKNFPLFCPKCRQEILIEITKFRITV
                     ITEPDAKTQSR"
     gene            complement(3349..3705)
                     /locus_tag="ACF3T5_002968"
     CDS             complement(3349..3705)
                     /locus_tag="ACF3T5_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFD3633448.1"
                     /translation="MWQTTLSNFDFKPLGLAIKEARLSKGLTREQVGSMIQIAPRYLT
                     NIENKGQQPSFHVVYDLVTLLNISLDGFILGEENSHKSSKRLQVEAQLDCLDEDELVT
                     IGKVIQAIYGEKCKFN"
BASE COUNT         1327 a          577 c          618 g         1197 t
ORIGIN      
        1 atttgatagt agatttaaaa ctttgcaaca gaaccctttt aatgatattt tccaagtcaa
       61 acttaatatt aacaattcaa aactattgga accactgcaa aatattttgt taagcagtat
      121 atatttcaaa aattatacgt aatatatttg tagaaaggtt gtggacagtt gtcctattga
      181 cctgaaaagt aatataatag ctggatgaat aaagaatccc ttatgaactt gtaaatcgtt
      241 attaaatcgc tgaattttga tagccattct gaagagttat cttcaactac tacactaact
      301 tccattaatt tctcattaat ttccctttcg ttcaaaaaat atggttggct ttacaagcat
      361 tctatcatat gatagaatta agataacatt caaaaggaga taataaaaat gcggcaaggt
      421 attcttaaat aaactgtcaa ttagatagcg ggaataatta attaagcatc cttatttaaa
      481 ataaggactg gttttttgtg cccagtttaa gaatactttt atcattttat cataaagcat
      541 ctgtggctat cagtgtgtag tatacagcta tttttgtatt aaaatcccag tgataagagt
      601 atttatcact gggatttttt ctgctttttg agcttttgaa tggaggaaat aaaattgaaa
      661 attattaata tcggtatctt agcacatgtt gatgcaggaa aaactacttt gacagaaagc
      721 ttactataca gtagcggagc aattaaagag ttaggaagtg tagatagcgg tacaacgaaa
      781 acggatacta tgtttttgga acgccagaga ggtattacta ttcagaccgc aataacatct
      841 tttcaacggg aaaatgttaa agtaaatatt gtagatactc ctggacacat ggattttttg
      901 gcagatgtat accgttcatt atctgttttg gatggagcta ttttgctaat ctctgcaaaa
      961 gatggagtac agtcacaaac tcgtatacta ttccatgcac ttagaaagat gaacatacct
     1021 ataatatttt ttattaacaa aattgatcaa aatggaataa atttgccaga tgtttatcaa
     1081 gatattaagg acaaactttc tgacgacatc ataattaagc agactgtgaa tctaaatttg
     1141 aaaccttatg taatagatta tactgaacca gaacaatggg agacagtaat tgtgggaaat
     1201 gattatttat tagaaaaata taccattggg aaaacattga atattgcaga acttgaaaag
     1261 gaggaaaacg aaagaattca aagttgctcc ttatatcctg tttatcacgg aagtgcaaag
     1321 aataatattg gaattaaaca acttatagag gtaattacta gcaaattatt ttcacccaca
     1381 caactcaatt cagataaact ttgtggaaat gtttttaaag tagaatattc agatgatggt
     1441 caacggcttg tctatgtacg tctttatagt ggaacgctac atttgcgaga ctcagtcaat
     1501 atatcagaaa aggaaaaaat aaaagttaca gaaatgtata cttcaataaa tggagaatta
     1561 cgccagatag ataaggcaga gcctggtgag attattattt taaaaaatga gcttttaaaa
     1621 ctaaataacg tacttggaga taaaaaaaga ttaccacata gagaaattct tgagaatcct
     1681 cttcctatgt tacaaacaac aattgaacca tgtaaatcag tacaaagaga aaagttacta
     1741 gatgcacttt ttgaaatatc cgatagtgat ccccttctac aatattatgt agatacagta
     1801 actcacgaaa ttgtgctatc ttttttaggt gaggtccaaa tggaggtaac ttgtactctg
     1861 attcaagaaa aatatcatat tgagatagaa acaagaaaac caactgtcat ttatatggaa
     1921 agaccattaa aaaaatctga atttaccatt gatatcgaag tacctccaaa tcctttctgg
     1981 gcttctattg gtttatctgt aacaccactt cctttgggta gtggcattca gtatgagagc
     2041 ctggtttctc taggttattt aaatcaatca tttcaaaatg cagttatgga aggtatacgc
     2101 tatgggtgtg aacaaggatt gtacggttgg aaattaacag actgtaagat ctgttttaag
     2161 tatggtctat attacagccc tgtcagtacg ccagcagatt tccgaatgct tgcgcctatt
     2221 gtactagagc aggcttttag aaagagtggt acagagttat tagagccata tcttagcttc
     2281 gaaatttatg taccacaaga atatctttcg agagcatata atgatgcttc caaatattgt
     2341 gcaaatattt taaatactaa gttaaaaggt aacgaggtca ttctcattgg tgaaattcca
     2401 gcccgttgta ttcaagagta tcgaaacagt ttaactttct ttacaaatgg acgcagtgtc
     2461 tgtttaacag agttaaaagg ttatcaggtt actaacatta agtctgcttt ccaaccacgt
     2521 cgtccaaata atagaataga caaagtaagg catatgttta ataaaatcaa cttacattga
     2581 tagtgaacta aagagaaatt tttaaaatga atattgagtt gttaattaga acaggaggat
     2641 atagatattg taaatactag gtggatactg tgccctatat gtggcaataa gacccgttta
     2701 aaaatgaggg aagatactga attaaaaaat ttccctctat tttgtcctaa atgcagacaa
     2761 gaaattttaa ttgaaataac gaagtttaga ataactgtaa ttacagagcc agacgcaaag
     2821 acgcagagcc gataattcga gatatcaaaa tctcatttta tcggcttttt cttttctatg
     2881 cataccattg agcttagttc ttgatatcct ttataagaaa ataagtgtac tgattttttt
     2941 tgcagtagct gtattacagt agacatccct caagcataaa aagtatacaa ttaaaattca
     3001 atacttgatt attgatgaag tacagagtta ctcgtcattt caaaactagt atatcagtca
     3061 agtttttcct aaggaacaaa aacgaacttc gctatgggaa tcaaaaattt ttagtcggat
     3121 gaagtgatat gcaataaatc cagaaaattt tcccaacaac aaaaaatgaa ctgtactttg
     3181 cagcgataag agtaaccaaa gaactattta tcattcgctc tagttcctaa gtgtaacaaa
     3241 gataaattca ttttctattt atgattaaca aaaatcagaa aaaattcatt acatctttgt
     3301 aatcaatctt tcctgatagt attaattaat ccattgtgta ttattgaact agttaaattt
     3361 acatttttct ccgtaaattg cttgaatcac tttcccaatc gtcactaatt catcttcatc
     3421 cagacaatcc aactgtgctt ctacttgaag acgtttggag cttttatgac tgttttcttc
     3481 ccctaaaata aatccatcca aagaaatatt tagcaaagtc actaaatcat atacaacatg
     3541 aaaacttggc tgttgtcctt tattttcaat gttcgtcaaa tagcgtggcg caatctggat
     3601 cattgatcct acctgctcac gagtcaatcc tttactaaga cgggcttctt taatcgcaag
     3661 acccaatggt ttgaaatcaa aatttgatag agtagtttgc catattcttg cacccaacg
//
LOCUS       DAXLPI010000366         3700 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.169, whole genome shotgun sequence.
ACCESSION   DAXLPI010000366 DAXLPI010000000
VERSION     DAXLPI010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.169"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..817
                     /locus_tag="ACF3T5_002969"
     CDS             <1..817
                     /locus_tag="ACF3T5_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HFD3633449.1"
                     /translation="LFDSRFKTLQQNPTTHEIILSFLGKVQMEVISALLQEKYHVEIE
                     LKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLN
                     QSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVL
                     KKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCI
                     QEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            924..977
                     /locus_tag="ACF3T5_002970"
                     /pseudo
     CDS             924..977
                     /locus_tag="ACF3T5_002970"
                     /inference="COORDINATES: protein motif:HMM:NF019662.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            1011..2387
                     /gene="tet(L)"
                     /locus_tag="ACF3T5_002971"
     CDS             1011..2387
                     /gene="tet(L)"
                     /locus_tag="ACF3T5_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HFD3633450.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            2951..>3700
                     /gene="mobV"
                     /locus_tag="ACF3T5_002972"
     CDS             2951..>3700
                     /gene="mobV"
                     /locus_tag="ACF3T5_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="HFD3633451.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGK"
BASE COUNT         1182 a          562 c          760 g         1196 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccct acgacacatg aaattatact
       61 ttctttctta gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca
      121 tgtggagata gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc
      181 agaatatacc attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc
      241 tgtatcaccg cttccgttgg gaagtggaat gcagtatgag agctcggttt ctcttggata
      301 cttaaatcaa tcatttcaaa atgcagttat ggaagggata cgctatggtt gcgaacaagg
      361 attatatggt tggaatgtga cggattgtaa aatctgtttt aagtatggct tatactatag
      421 ccctgttagt accccagcag attttcggat gcttgctcct attgtattgg aacaagtctt
      481 aaaaaaagct ggaacagaat tgttagagcc atatcttagt tttaaaattt atgcgccaca
      541 ggaatatctt tcacgagcat acaacgatgc tcctaaatat tgtgcgaaca tcgtagacac
      601 tcaattgaaa aataatgagg tcattcttag tggagaaatc cctgctcggt gtattcaaga
      661 atatcgtagt gatttaactt tctttacaaa tggacgtagt gtttgtttaa cagagttaaa
      721 agggtaccat gttactaccg gtgaacctgt ttgccagccc cgtcgtccaa atagtcggat
      781 agataaagta cgatatatgt tcaataaaat aacttagtgt attttatgtt gttatataaa
      841 tatggtttct tgttaaataa gatgaaatat tttttaataa agatttgaat taaagtgtaa
      901 aggaggaatt aattatgaag tgaaatgaat gtaacagggt tcaattaaaa gagggaagcg
      961 tatcattaac cctataaact acgtctgccc tcattattgg agggtgaaat gtgaatacat
     1021 cctattcaca atcgaattta cgacacaacc aaattttaat ttggctttgc attttatctt
     1081 tttttagcgt attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt
     1141 ttaataaacc acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca
     1201 ttggaacagc tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat
     1261 ttggaattat aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt
     1321 ccttacttat tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg
     1381 taatggttgt agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta
     1441 ttggatcgat agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc
     1501 attatattca ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt
     1561 ttcttatgaa attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa
     1621 ttatactaat gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt
     1681 cttttcttat cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa
     1741 cagatccttt tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt
     1801 gtgggggaat tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga
     1861 aagatgttca ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa
     1921 tgagtgtcat tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat
     1981 acgtgttaaa catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt
     2041 tagaaacaac atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt
     2101 tcaccaaaac agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg
     2161 ctggaatgag tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg
     2221 taggtggttt attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc
     2281 agtcaactta tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt
     2341 ggctggttac cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat
     2401 caactttggg agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt
     2461 gttccattaa agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt
     2521 ttattttgag tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc
     2581 ttgctgagtc tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt
     2641 ttaaaggtgg agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt
     2701 tttaaacgag cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt
     2761 gtttcttttt tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac
     2821 tgccgacagc ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta
     2881 cttggaagtg gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag
     2941 cgatttacat atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa
     3001 aggcatgcaa tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca
     3061 tgagcgaaca cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg
     3121 tgtcaaagaa attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt
     3181 tcttgtaaat gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg
     3241 agaacaaaaa cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca
     3301 aaatattgct tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt
     3361 tgtgcctatg cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt
     3421 atggctacaa gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg
     3481 tgaacgtggc tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga
     3541 aaaagagatt gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta
     3601 tagcgataaa gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga
     3661 agtgccaacg ggtgaaaagt ccatgtttgg tttgggaaaa
//
LOCUS       DAXLPI010000367         2610 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.195, whole genome shotgun sequence.
ACCESSION   DAXLPI010000367 DAXLPI010000000
VERSION     DAXLPI010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.195"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(530..655)
                     /locus_tag="ACF3T5_002973"
     CDS             complement(530..655)
                     /locus_tag="ACF3T5_002973"
                     /inference="COORDINATES: protein motif:HMM:NF019687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633452.1"
                     /translation="MLFPPFKSSKSRKNPSDKYSYHWDFNTKIAVYYTLIATDAL"
     gene            640..2592
                     /gene="tet(S)"
                     /locus_tag="ACF3T5_002974"
     CDS             640..2592
                     /gene="tet(S)"
                     /locus_tag="ACF3T5_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(S)"
                     /protein_id="HFD3633453.1"
                     /translation="MEEIKLKIINIGILAHVDAGKTTLTESLLYSSGAIKELGSVDSG
                     TTKTDTMFLERQRGITIQTAITSFQRENVKVNIVDTPGHMDFLADVYRSLSVLDGAIL
                     LISAKDGVQSQTRILFHALRKMNIPIIFFINKIDQNGINLPDVYQDIKDKLSDDIIIK
                     QTVNLNLKPYVIDYTEPEQWETVIVGNDYLLEKYTIGKTLNIAELEKEENERIQSCSL
                     YPVYHGSAKNNIGIKQLIEVITSKLFSPTQLNSDKLCGNVFKVEYSDDGQRLVYVRLY
                     SGTLHLRDSVNISEKEKIKVTEMYTSINGELRQIDKAEPGEIIILKNELLKLNNVLGD
                     KKRLPHREILENPLPMLQTTIEPCKSVQREKLLDALFEISDSDPLLQYYVDTVTHEIV
                     LSFLGEVQMEVTCTLIQEKYHIEIETRKPTVIYMERPLKKSEFTIDIEVPPNPFWASI
                     GLSVTPLPLGSGIQYESLVSLGYLNQSFQNAVMEGIRYGCEQGLYGWKLTDCKICFKY
                     GLYYSPVSTPADFRMLAPIVLEQAFRKSGTELLEPYLSFEIYVPQEYLSRAYNDASKY
                     CANILNTKLKGNEVILIGEIPARCIQEYRNSLTFFTNGRSVCLTELKGYQVTNIKSAF
                     QPRRPNNRIDKVRHMFNKINGSVAKF"
BASE COUNT          940 a          386 c          453 g          831 t
ORIGIN      
        1 atttgatagt agatttaaaa ctttgcaaca gaaccctttt aatgatattt tccaagtcaa
       61 acttaatatt aacaattcaa aactattgga accactgcaa aatattttgt taagcagtat
      121 atatttcaaa aattatacgt aatatatttg tagaaaggtt gtggacagtt gtcctattga
      181 cctgaaaagt aatataatag ctggatgaat aaagaatccc ttatgaactt gtaaatcgtt
      241 attaaatcgc tgaattttga tagccattct gaagagttat cttcaactac tacactaact
      301 tccattaatt tctcattaat ttccctttcg ttcaaaaaat atggttggct ttacaagcat
      361 tctatcatat gatagaatta agataacatt caaaaggaga taataaaaat gcggcaaggt
      421 attcttaaat aaactgtcaa ttagatagcg ggaataatta attaagcatc cttatttaaa
      481 ataaggactg gttttttgtg cccagtttaa gaatactttt atcattttat cataaagcat
      541 ctgtggctat cagtgtgtag tatacagcta tttttgtatt aaaatcccag tgataagagt
      601 atttatcact gggatttttt ctgctttttg agcttttgaa tggaggaaat aaaattgaaa
      661 attattaata tcggtatctt agcacatgtt gatgcaggaa aaactacttt gacagaaagc
      721 ttactataca gtagcggagc aattaaagag ttaggaagtg tagatagcgg tacaacgaaa
      781 acggatacta tgtttttgga acgccagaga ggtattacta ttcagaccgc aataacatct
      841 tttcaacggg aaaatgttaa agtaaatatt gtagatactc ctggacacat ggattttttg
      901 gcagatgtat accgttcatt atctgttttg gatggagcta ttttgctaat ctctgcaaaa
      961 gatggagtac agtcacaaac tcgtatacta ttccatgcac ttagaaagat gaacatacct
     1021 ataatatttt ttattaacaa aattgatcaa aatggaataa atttgccaga tgtttatcaa
     1081 gatattaagg acaaactttc tgacgacatc ataattaagc agactgtgaa tctaaatttg
     1141 aaaccttatg taatagatta tactgaacca gaacaatggg agacagtaat tgtgggaaat
     1201 gattatttat tagaaaaata taccattggg aaaacattga atattgcaga acttgaaaag
     1261 gaggaaaacg aaagaattca aagttgctcc ttatatcctg tttatcacgg aagtgcaaag
     1321 aataatattg gaattaaaca acttatagag gtaattacta gcaaattatt ttcacccaca
     1381 caactcaatt cagataaact ttgtggaaat gtttttaaag tagaatattc agatgatggt
     1441 caacggcttg tctatgtacg tctttatagt ggaacgctac atttgcgaga ctcagtcaat
     1501 atatcagaaa aggaaaaaat aaaagttaca gaaatgtata cttcaataaa tggagaatta
     1561 cgccagatag ataaggcaga gcctggtgag attattattt taaaaaatga gcttttaaaa
     1621 ctaaataacg tacttggaga taaaaaaaga ttaccacata gagaaattct tgagaatcct
     1681 cttcctatgt tacaaacaac aattgaacca tgtaaatcag tacaaagaga aaagttacta
     1741 gatgcacttt ttgaaatatc cgatagtgat ccccttctac aatattatgt agatacagta
     1801 actcacgaaa ttgtgctatc ttttttaggt gaggtccaaa tggaggtaac ttgtactctg
     1861 attcaagaaa aatatcatat tgagatagaa acaagaaaac caactgtcat ttatatggaa
     1921 agaccattaa aaaaatctga atttaccatt gatatcgaag tacctccaaa tcctttctgg
     1981 gcttctattg gtttatctgt aacaccactt cctttgggta gtggcattca gtatgagagc
     2041 ctggtttctc taggttattt aaatcaatca tttcaaaatg cagttatgga aggtatacgc
     2101 tatgggtgtg aacaaggatt gtacggttgg aaattaacag actgtaagat ctgttttaag
     2161 tatggtctat attacagccc tgtcagtacg ccagcagatt tccgaatgct tgcgcctatt
     2221 gtactagagc aggcttttag aaagagtggt acagagttat tagagccata tcttagcttc
     2281 gaaatttatg taccacaaga atatctttcg agagcatata atgatgcttc caaatattgt
     2341 gcaaatattt taaatactaa gttaaaaggt aacgaggtca ttctcattgg tgaaattcca
     2401 gcccgttgta ttcaagagta tcgaaacagt ttaactttct ttacaaatgg acgcagtgtc
     2461 tgtttaacag agttaaaagg ttatcaggtt actaacatta agtctgcttt ccaaccacgt
     2521 cgtccaaata atagaataga caaagtaagg catatgttta ataaaatcaa cggttctgtt
     2581 gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DAXLPI010000368         2566 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.197, whole genome shotgun sequence.
ACCESSION   DAXLPI010000368 DAXLPI010000000
VERSION     DAXLPI010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.197"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            complement(39..341)
                     /locus_tag="ACF3T5_002975"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="ACF3T5_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
     gene            434..783
                     /locus_tag="ACF3T5_002976"
                     /pseudo
     CDS             434..783
                     /locus_tag="ACF3T5_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1055..1345)
                     /locus_tag="ACF3T5_002977"
     CDS             complement(1055..1345)
                     /locus_tag="ACF3T5_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFD3633454.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1553..2245)
                     /locus_tag="ACF3T5_002978"
     CDS             complement(1553..2245)
                     /locus_tag="ACF3T5_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFD3633455.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          759 a          472 c          357 g          978 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagatta ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac
     1561 gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg
     1621 ctttttctaa cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta
     1681 atttcttttg tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg
     1741 cgatttgttg attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt
     1801 cttgtagtct tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga
     1861 tctcatctag tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga
     1921 ctaattgact ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt
     1981 catttacttt tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt
     2041 tttgtgctag tcgagcggta agatctgtaa tcgcttgctc tttatctgtc aatgtatgat
     2101 caagctgtgt cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa
     2161 tttcatttgc gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc
     2221 caccagtaat aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct
     2281 aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa attacatatg taattttaat
     2341 ataaatacta tcaaatactc atttaatcct ccttttttat ctatttaaaa aatctttttt
     2401 atagagaaaa atgcaggtta atactttctt aaataaatat ttatggtgaa caaaaacaaa
     2461 aaagcaagtt ttattaaacg taaccctttt catttcatag attactgtat ttattacaaa
     2521 aaacgcggat ttgtcataaa ttgtcatgct tcttttacct atgaaa
//
LOCUS       DAXLPI010000369         2558 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.199, whole genome shotgun sequence.
ACCESSION   DAXLPI010000369 DAXLPI010000000
VERSION     DAXLPI010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.199"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..173
                     /locus_tag="ACF3T5_002979"
     CDS             <1..173
                     /locus_tag="ACF3T5_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFD3633456.1"
                     /translation="KYHRTLTTYLNTLLSNSFIINQIVEPQPPENMMDIPGMADEMRR
                     PMMLIVSAKKKM"
     gene            206..1114
                     /locus_tag="ACF3T5_002980"
     CDS             206..1114
                     /locus_tag="ACF3T5_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HFD3633457.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1111..1653
                     /gene="sat4"
                     /locus_tag="ACF3T5_002981"
     CDS             1111..1653
                     /gene="sat4"
                     /locus_tag="ACF3T5_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HFD3633458.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            1746..2540
                     /locus_tag="ACF3T5_002982"
                     /note="aph(3')-IIIa"
     CDS             1746..2540
                     /locus_tag="ACF3T5_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HFD3633459.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          872 a          398 c          603 g          685 t
ORIGIN      
        1 caaaatatca tagaacactg accacatatc taaatacact gctttcaaat agttttataa
       61 taaatcagat tgtggagcca cagccgccag agaacatgat ggatattccg gggatggcgg
      121 atgaaatgcg acgcccaatg atgctgattg tatcggcaaa aaagaagatg taataatata
      181 gaaaaaataa acgaggagta tgtaaatgag atcagaaaaa gaaatgatgg atttagtact
      241 ttctttagca gaacaggatg aacgtattcg aattgtgacc cttgaggggt cacgcgcaaa
      301 tattaatata cctaaagatg aatttcagga ttatgatatt acatattttg taagtgatat
      361 agaaccgttt atatctaatg atgactggct taatcaattt gggaatataa taatgatgca
      421 aaagccggag gatatggaat tattcccacc tgaagaaaag ggattttcct atcttatgct
      481 atttgatgat tacaataaaa ttgatcttac cttattgccc ttggaagagt tagataatta
      541 cctaaagggc gataaattaa taaaggttct aattgataaa gattgtagaa ttaaaaggga
      601 catagttccg actgatatag attatcatgt aagaaagcca agcgcaaggg agtatgatga
      661 ttgctgcaat gaattttgga atgtaacacc ttatgttatt aaaggattgt gccgtaagga
      721 aattttattt gctattgatc attttaatca gattgttcgc catgagctgc tgagaatgat
      781 atcatggaag gtcggcatcg aaacaggctt taaattaagt gtaggcaaga actataagtt
      841 tattgaaagg tatatatccg aggatttgtg ggagaaactt ttgtccacct accggatgga
      901 ttcctatgaa aacatatggg aagcattatt tctatgccat caattgttca gggcggtatc
      961 cggtgaggtg gcggaaaggc ttcattatgc ctatccggag tatgatagga atataacaaa
     1021 atataccagg gacatgtata aaaaatacac tggtaaaacc ggctgcctgg atagcacata
     1081 tgccgctgat atagaagaga ggcgggaaca gtgattacag aaatgaaagc agggcacctg
     1141 aaagatatcg ataaacccag cgaaccattt gaggtgatag gtaagattat accgaggtat
     1201 gaaaacgaga attggacctt tacagaatta ctctatgaag cgccatattt aaaaagctac
     1261 caagacgaag aggatgaaga ggatgaggag gcagattgcc ttgaatatat tgacaatact
     1321 gataagataa tatatcttta ctaccaagac gataaatgcg tcggaaaagt taaactgcga
     1381 aaaaattgga accggtacgc ttatatagaa gatatcgccg tatgtaagga tttcaggggg
     1441 caaggcatag gcagcgcgct tatcaatata tctatagaat gggcaaagca taaaaacttg
     1501 catggactaa tgcttgaaac ccaggacaat aaccttatag cttgtaaatt ctatcataat
     1561 tgtggtttca aaatcggctc cgtcgatact atgttatacg ccaactttga aaacaacttt
     1621 gaaaaagctg ttttctggta tttaaggttt tagaatgcaa ggaacagtga attggagttc
     1681 gtcttgttat aattagcttc ttggggtatc tttaaatact gtagaaaaga ggaaggaaat
     1741 aataaatggc taaaatgaga atatcaccgg aattgaaaaa actgatcgaa aaataccgct
     1801 gcgtaaaaga tacggaagga atgtctcctg ctaaggtata taagctggtg ggagaaaatg
     1861 aaaacctata tttaaaaatg acggacagcc ggtataaagg gaccacctat gatgtggaac
     1921 gggaaaagga catgatgcta tggctggaag gaaagctgcc tgttccaaag gtcctgcact
     1981 ttgaacggca tgatggctgg agcaatctgc tcatgagtga ggccgatggc gtcctttgct
     2041 cggaagagta tgaagatgaa caaagccctg aaaagattat cgagctgtat gcggagtgca
     2101 tcaggctctt tcactccatc gacatatcgg attgtcccta tacgaatagc ttagacagcc
     2161 gcttagccga attggattac ttactgaata acgatctggc cgatgtggat tgcgaaaact
     2221 gggaagaaga cactccattt aaagatccgc gcgagctgta tgatttttta aagacggaaa
     2281 agcccgaaga ggaacttgtc ttttcccacg gcgacctggg agacagcaac atctttgtga
     2341 aagatggcaa agtaagtggc tttattgatc ttgggagaag cggcagggcg gacaagtggt
     2401 atgacattgc cttctgcgtc cggtcgatca gggaggatat cggggaagaa cagtatgtcg
     2461 agctattttt tgacttactg gggatcaagc ctgattggga gaaaataaaa tattatattt
     2521 tactggatga attgttttag tacctagatt tagatgtc
//
LOCUS       DAXLPI010000370          798 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.297, whole genome shotgun sequence.
ACCESSION   DAXLPI010000370 DAXLPI010000000
VERSION     DAXLPI010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.297"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            44..781
                     /gene="erm(B)"
                     /locus_tag="ACF3T5_002983"
     CDS             44..781
                     /gene="erm(B)"
                     /locus_tag="ACF3T5_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFD3633460.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          293 a          139 c          125 g          241 t
ORIGIN      
        1 ttctgtattt gtttatagat gtaatcactt caggagtgat aacatgaaca aaaatataaa
       61 atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa aacaattgaa
      121 tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt taacgacgaa
      181 actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc tattcaactt
      241 atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag atattctaca
      301 gtttcaattc cctaacaaac agaggtataa aattgttggg aatattcctt accatttaag
      361 cacacaaatt attaaaaaag tggtttttga aagccgtgcg tctgacatct atctgattgt
      421 tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt tgctcttgca
      481 cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc atcctaaacc
      541 aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc cagataaata
      601 ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt
      661 tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt
      721 tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg ggaggaaata
      781 attctatgag tcgctttt
//
LOCUS       DAXLPI010000371          798 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.298, whole genome shotgun sequence.
ACCESSION   DAXLPI010000371 DAXLPI010000000
VERSION     DAXLPI010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.298"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            44..781
                     /gene="erm(B)"
                     /locus_tag="ACF3T5_002984"
     CDS             44..781
                     /gene="erm(B)"
                     /locus_tag="ACF3T5_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFD3633461.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKYA
                     KVNDLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          292 a          138 c          126 g          242 t
ORIGIN      
        1 ttctgtattt gtttatagat gtaatcactt caggagtgat aacatgaaca aaaatataaa
       61 atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa aacaattgaa
      121 tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt taacgacgaa
      181 actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc tattcaactt
      241 atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag atattctaca
      301 gtttcaattc cctaacaaac agaggtataa aattgttggg aatattcctt accatttaag
      361 cacacaaatt attaaaaaag tggtttttga aagccgtgcg tctgacatct atctgattgt
      421 tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt tgctcttgca
      481 cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc atcctaaacc
      541 aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc cagataaata
      601 ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt
      661 tactaaaaat cagtttcatc aagcaatgaa atacgccaaa gtaaacgatt taagtaccgt
      721 tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg ggaggaaata
      781 attctatgag tcgctttt
//
LOCUS       DAXLPI010000372          733 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.305, whole genome shotgun sequence.
ACCESSION   DAXLPI010000372 DAXLPI010000000
VERSION     DAXLPI010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.305"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>733
                     /gene="aac(6')-I"
                     /locus_tag="ACF3T5_002985"
     CDS             <1..>733
                     /gene="aac(6')-I"
                     /locus_tag="ACF3T5_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ie family aminoglycoside
                     N-acetyltransferase"
                     /protein_id="HFD3633462.1"
                     /translation="IDDDFPLMLKWLTDERVLEFYDGRDKKYTLESLKKHYTEPWEDE
                     VFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFIGEPNYWSKGIGTR
                     YIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLPEHELHEGKKEDCY
                     LMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFST
                     DKKKGYAKEKAIYNFLNTNLETNVKI"
BASE COUNT          309 a           59 c          127 g          238 t
ORIGIN      
        1 atagatgatg attttccttt gatgttaaaa tggttaactg atgaaagagt attagaattt
       61 tatgatggta gagataaaaa atatacatta gaatcattaa aaaaacatta tacagagcct
      121 tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat tggatatgga
      181 caaatatata aaatgtatga tgagttatat actgattatc attatccaaa aactgatgag
      241 atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa aggaattggt
      301 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
      361 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      421 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      481 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      541 gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag tggttatgat
      601 agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag tactgataag
      661 aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact
      721 aatgtaaaaa ttc
//
LOCUS       DAXLPI010000373          640 bp    DNA     linear   BCT 08-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium strain JARB-OU2434
            SAMD00565623-rid20744943.guided.315, whole genome shotgun sequence.
ACCESSION   DAXLPI010000373 DAXLPI010000000
VERSION     DAXLPI010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMD00565623
            Sequence Read Archive: DRR428009
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 16-JAN-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002054648.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002054648.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/08/2024 14:39:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,986
            CDSs (total)                      :: 2,919
            Genes (coding)                    :: 2,816
            CDSs (with protein)               :: 2,816
            Genes (RNA)                       :: 67
            rRNAs                             :: 1, 3, 3 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 44 of 103
            Pseudo Genes (incomplete)         :: 52 of 103
            Pseudo Genes (internal stop)      :: 27 of 103
            Pseudo Genes (multiple problems)  :: 17 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMD00565623-rid20744943.guided.315"
                     /strain="JARB-OU2434"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Japan: Hiroshima"
                     /collection_date="2020-12-11"
                     /collected_by="Hiroshima"
     gene            <1..>640
                     /locus_tag="ACF3T5_002986"
     CDS             <1..>640
                     /locus_tag="ACF3T5_002986"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFD3633463.1"
                     /translation="NIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDI
                     ASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNA
                     TTVFEGKKCLCHNDFSCSHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIG
                     TNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKE"
BASE COUNT          266 a           44 c          113 g          217 t
ORIGIN      
        1 taatattgaa tattcgtata ttagtgatga attatctata ctaggttata aagaaattaa
       61 aggaactttt ttaacaccag aaatttattc tactatgtca gaagaagaac aaaatttgtt
      121 aaaacgagat attgccagtt ttttaagaca aatgcacggt ttagattata cagatattag
      181 tgaatgtact attgataata aacaaaatgt attagaagag tatatattgt tgcgtgaaac
      241 tatttataat gatttaactg atatagaaaa agattatata gaaagtttta tggaaagact
      301 aaatgcaaca acagtttttg agggtaaaaa gtgtttatgc cataatgatt ttagttgtag
      361 tcatctattg ttagatggca ataatagatt aactggaata attgattttg gagattctgg
      421 aattatagat gaatattgtg attttatata cttacttgaa gatagtgaag aagaaatagg
      481 aacaaatttt ggagaagata tattaagaat gtatggaaat atagatattg agaaagcaaa
      541 agaatatcaa gatatagttg aagaatatta tcctattgaa actattgttt atggaattaa
      601 aaatattaaa caggaattta tcgaaaatgg tagaaaagaa
//
